LOCUS       DAWEWP010000001        35837 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWEWP010000001 DAWEWP010000000
VERSION     DAWEWP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35837
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.001"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            81..992
                     /locus_tag="WGN53_000001"
     CDS             81..992
                     /locus_tag="WGN53_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0139054.1"
                     /translation="MLRLTALTLCTVLALGACSPQNSDSAPQAKEQAVSAAQSESASV
                     TVKTVRGDAKVPQNPERIAVYDLGMLDTLNKLGVKTGLSVDTNRLPYLEEYYKTTKPA
                     GTLFEPDYEALNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKRQVVYLVPETYLAAGGA"
     gene            1210..2178
                     /locus_tag="WGN53_000002"
     CDS             1210..2178
                     /locus_tag="WGN53_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0139055.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2168..3142
                     /locus_tag="WGN53_000003"
     CDS             2168..3142
                     /locus_tag="WGN53_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ0139056.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELAVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCIGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLRHKK"
     gene            3187..3813
                     /locus_tag="WGN53_000004"
     CDS             3187..3813
                     /locus_tag="WGN53_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139057.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGVGEINGTLTLRRKDNGKTVAVGLNAALQPFAPE
                     MRDIMPKAVSGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3896..4654
                     /locus_tag="WGN53_000005"
     CDS             3896..4654
                     /locus_tag="WGN53_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0139058.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLADTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPNDIFTAANIKTLFDMDVDVLDYEGKKLVIHHI"
     gene            4946..6163
                     /locus_tag="WGN53_000006"
     CDS             4946..6163
                     /locus_tag="WGN53_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HEZ0139059.1"
                     /translation="MKHFPSKVLTTAILATFCSGALAATNDDDVKKAATVAIAAAYNN
                     GQEINGFKAGETIYDIDEDGTITKKDATAADVEADDFKGLGLKKVVTNLTKTVNENKQ
                     NVDAKVKAAESEIEKLTTKLADTDAALADTDAALDATTNALNKLGENITTFAEETKTN
                     IVKIDEKLEAVADTVDKHAEAFNDIADSLDETNTKADEAVKTANEAKQTAEETKQNVD
                     AKVKAAETAAGKAEAAAGTANTAADKAEAVAAKVTDIKADIATNKDNIAKKANSADVY
                     TREESDSKFVRIDGLNATTEKLDTRLASAEKSITEHGTRLNGLDRTVSDLRKETRQGL
                     AEQAALSGLFQPYNVGRFNVTAAVGGYKSESAVAIGTGFRFTENFAAKAGVAVGTSSG
                     SSAAYHVGVNYEW"
     gene            complement(6229..6567)
                     /locus_tag="WGN53_000007"
     CDS             complement(6229..6567)
                     /locus_tag="WGN53_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ0139060.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            6718..10680
                     /gene="purL"
                     /locus_tag="WGN53_000008"
     CDS             6718..10680
                     /gene="purL"
                     /locus_tag="WGN53_000008"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ0139061.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPIDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLHLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            10775..11533
                     /gene="gloB"
                     /locus_tag="WGN53_000009"
     CDS             10775..11533
                     /gene="gloB"
                     /locus_tag="WGN53_000009"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ0139062.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            11847..15066
                     /locus_tag="WGN53_000010"
                     /pseudo
     CDS             11847..15066
                     /locus_tag="WGN53_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254770.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            15142..16323
                     /locus_tag="WGN53_000011"
     CDS             15142..16323
                     /locus_tag="WGN53_000011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ0139063.1"
                     /translation="MKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFE
                     TELDTYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIR
                     MPNLAELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHS
                     GTHRPYQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVK
                     GENGALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRI
                     NRSNGNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSG
                     SALSVNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYN
                     W"
     gene            16555..18009
                     /gene="mgtE"
                     /locus_tag="WGN53_000012"
     CDS             16555..18009
                     /gene="mgtE"
                     /locus_tag="WGN53_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ0139064.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(18095..19003)
                     /gene="hslO"
                     /locus_tag="WGN53_000013"
     CDS             complement(18095..19003)
                     /gene="hslO"
                     /locus_tag="WGN53_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ0139065.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENEWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIGEDESLVDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            19248..20003
                     /locus_tag="WGN53_000014"
     CDS             19248..20003
                     /locus_tag="WGN53_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0139066.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            20022..20240
                     /locus_tag="WGN53_000015"
     CDS             20022..20240
                     /locus_tag="WGN53_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139067.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            20385..20729
                     /locus_tag="WGN53_000016"
     CDS             20385..20729
                     /locus_tag="WGN53_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ0139068.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPDSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            20729..21421
                     /locus_tag="WGN53_000017"
     CDS             20729..21421
                     /locus_tag="WGN53_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ0139069.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVIVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            21488..22708
                     /gene="argJ"
                     /locus_tag="WGN53_000018"
     CDS             21488..22708
                     /gene="argJ"
                     /locus_tag="WGN53_000018"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ0139070.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVVD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQSAATVYTCD
                     LSHGYVSINADYRS"
     gene            22768..24177
                     /locus_tag="WGN53_000019"
     CDS             22768..24177
                     /locus_tag="WGN53_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0139071.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGVSIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            24366..25757
                     /gene="hrpA"
                     /locus_tag="WGN53_000020"
     CDS             24366..25757
                     /gene="hrpA"
                     /locus_tag="WGN53_000020"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ0139072.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     VAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIRETAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            25858..26985
                     /locus_tag="WGN53_000021"
     CDS             25858..26985
                     /locus_tag="WGN53_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139073.1"
                     /translation="MKHLLIDFENVQPQNLDKLPTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            27163..28740
                     /gene="lpt3"
                     /locus_tag="WGN53_000022"
     CDS             27163..28740
                     /gene="lpt3"
                     /locus_tag="WGN53_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ0139074.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKATRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGG
                     AGASMLDKLWLPALWGMLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQSAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            28842..32105
                     /locus_tag="WGN53_000023"
     CDS             28842..32105
                     /locus_tag="WGN53_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ0139075.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRLSEIKQLTSSENQGDQDLSAKRKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTKLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVPYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYATQQPTPSPAREGRGEGKTVAAQTNFSATAANPLPQERE
                     QSAAASAVSNDLHPANPQQTAPSPVGEGWGEGKTVATQTNFSATTANPLPNPLPQEGE
                     QSAAASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWLKTAERENPRLLFLSRDDL
                     MQHAAAHITEEQFPKFWQTADGKFELSYRFEPHHPLDGVTLTLPLTVLNRISPAALEW
                     LVPGMLREKIQLLIKALPKQIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTA
                     GDIRIFEQINQDEWAAFRLPEHCYFNLRIIDDGGQELAIGRDLIQIQQQLGQAAAVTF
                     RDNTQEFERDNVTTWDIGILPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTIEAA
                     EQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAF
                     IGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNSKLGKHPLTHLL
                     RQRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQM
                     WQEKTDSLIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKMWEDLN"
     gene            complement(32128..32550)
                     /locus_tag="WGN53_000024"
     CDS             complement(32128..32550)
                     /locus_tag="WGN53_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ0139076.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEILVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAIGRERLNRLVREIKDAV"
     gene            complement(32554..34200)
                     /locus_tag="WGN53_000025"
     CDS             complement(32554..34200)
                     /locus_tag="WGN53_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEZ0139077.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIKKSERIYQPEFQKESVEPNTENGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLSIDFIDNDDEDLIATDRTSLDWKHPKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIENWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFENKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(34214..35269)
                     /locus_tag="WGN53_000026"
     CDS             complement(34214..35269)
                     /locus_tag="WGN53_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0139078.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            complement(35669..>35837)
                     /locus_tag="WGN53_000027"
     CDS             complement(35669..>35837)
                     /locus_tag="WGN53_000027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139079.1"
                     /translation="VRESCTSNANPPLSELLKLINRQLTEKGLKIEKASAAVVDATII
                     QTAGSKQRQAR"
BASE COUNT         8988 a        10645 c         8523 g         7681 t
ORIGIN      
        1 ttatcattta tcctttctaa agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacacct atgttgcgtt tgaccgcatt gaccctctgc accgtcctcg
      121 ccttgggcgc gtgttcgccg caaaattccg actctgcccc acaagccaaa gaacaggcag
      181 tttccgccgc acaatccgaa agcgcgtccg ttaccgtcaa aaccgtgcgc ggcgatgcaa
      241 aagtgccgca aaaccccgaa cgcatcgccg tttacgattt gggtatgctc gacactttaa
      301 acaaattggg cgtgaaaacc ggtttgtccg tcgacacaaa ccgcctgccg tatttagagg
      361 aatattacaa aacaacgaaa cctgccggaa ctttgttcga gccggattac gaagcactca
      421 acgcctacaa gccgcagctc atcatcatcg gcagccgcgc agccaaggcg tttgacaaat
      481 tgaacgaaat cgcgccgacc atcgaaatga ccgccgatac cgccaacctc aaagaaagtg
      541 ccaaagaacg tatcgacgcg ctggcgcaaa tcttcggcaa acaggcggaa gcggacaagc
      601 tgaaggcgga aatcgacgcg tcttttgaag ccgcgaaaac tgccgcgcaa ggcaaaggca
      661 aaggtttggt gattttggtc aacggcggca agatgtccgc cttcggcccg tcttcacgac
      721 tgggcggctg gctgcacaaa gacatcggcg ttcccgctgt tgacgaagcc atcaaagaag
      781 gcagccacgg tcagcctatc agctttgaat acctgaaaga gaaaaatccc gactggctgt
      841 ttgtccttga ccgcagcgcg gccatcggcg aagagggtca ggcggcgaaa gacgtgttga
      901 ataatccgct ggttgccgaa acaaccgcct ggaaaaaaag acaagtcgtt tacctcgttc
      961 ctgaaactta tttggcggcc ggcggcgcgt aagagctgct gaatgcaagc aaacaggttg
     1021 cggatgcttt taacgccgcg aaataatgaa acggcggcat tcgatgccgt ctgaaacacg
     1081 gatgcaaacc gcttcctgtg tttcagacgg cattgcccga tacggaggct tcaaacaagg
     1141 ctttccgctc cgacggttcg gactgccttg tttgaatctt ctacgcctta acgcttttcc
     1201 cttctgttta tgactgccaa acctttttcc ctcaacctga ccaacctcct gctgctggcg
     1261 gtattgtttg ccgtcagcct gtcggtgggc gttgccgatt tccgctggtc ggatgtgttt
     1321 tcgctatccg acagccagca ggtcatgttc atcagccgcc tgccgcgcac gtttgcgatt
     1381 gtgctgacgg gcgcgtctat ggcggtggcg ggcatgatta tgcagattct gatgcgtaac
     1441 cgttttgtcg agccttctat ggcgggtgcg ggtcagagtg cggctttggg tttgcttctg
     1501 atgtccctgc tgctgcctgc cgcgccgctg ccggtcaaaa tgtcggttgc cgccgttgcc
     1561 gcgctaatcg ggatgctggt gtttatgctg ctgatccgcc gcctgccgcc gacggcgcaa
     1621 ctgatggtgc cgctggtggg gattattttc ggcggcgtgg ttgaggcggt ggcgacgttt
     1681 atcgcgtatg aaaacgaaat gctgcaaatg ctcggcgtgt ggcagcaggg cgatttttcg
     1741 agcgtgctgc tggggcggta cgagctgctt tggattacgg gcggtttggc ggtgtttgcc
     1801 tatctgattg ccgaccggct gacgattttg gggctgggcg agacggtcag cgtgaatttg
     1861 ggtttgaacc ggacggcggt gttgtggtcg gggctgatta ttgtagcact gattacatcg
     1921 ctggtcattg taacggtcgg caatattccg tttatcgggc tggtcgtgcc gaacatcatc
     1981 agccgcctga taggcgacag gctgcgccaa agcctgcctg cggtggcctt gttgggcgcg
     2041 tctttggttt tattgtgcga cattatcgga cgcgtgattg tgtttccgtt tgaaattccg
     2101 gtctctacgg tttttggtgt gttgggtacg gctttgtttt tgtggctttt gttaaggaaa
     2161 cctgctcatg ccgtctgaaa aaaatatcgg ttttatggca ggaagcagcc gtccgttgtg
     2221 ggttgccttt gcgctgttgc tggtttcctg catcctgttt atgacgctca acgtcaaagg
     2281 cgattgggac tttgtactgc acctgcgtct gaccaaactt gccgcgctgc tgatggtcgc
     2341 ctatgcggtc ggcgtgtcca cgcaactctt ccaaacgctg accaacaatc cgattctgac
     2401 cccttcaatt ttgggtttcg attcgctgta tgtgtttttg cagaccttgc tggtgtttac
     2461 gttcggcggc gtgggctatg cttccctgcc gttgacgggc aaattcggct ttgaacttgc
     2521 cgttatgatg ggcggctcgc tgctgctgtt ttacacgctc atccgtcagg gcggacgcga
     2581 tttgccgcgt atgattttaa tcggcgtgat tttcgggatt ttgttccgca gcctgtcgtc
     2641 gctgctttcg cgcatgatcg accccgaaga atttacggcg gcgcaggcga atatgtttgc
     2701 cggattcaat accgtccaca gcgagctttt gggcataggc gcgctgattc tgctcgtcag
     2761 cgcggcggtc gtttggcgcg aacgctaccg cttggacgta caccttttgg ggcgcgacca
     2821 agccgtcaat ttgggcatca gctacacgcg caacaccttg tggatactgc tttggattgc
     2881 cgcattggtg gcgacggcga ccgccgtggt cggcccggta agctttttcg ggcttctcgc
     2941 cgcctcgctt gccaaccact tttccccgtc cgtgcgccat tccgtccgcc tgccgatgac
     3001 ggtttgtatc ggcggtatcc tcttggtcgg cggacagacc gtattcgaac acttcttggg
     3061 catgaaggcg gtattaagcg tggtggtcga atttgcgggc ggactcgttt tcctctatct
     3121 cgttttaaga cacaaaaaat gacggatgcc atctgaacgg cagcccgccc cgaaaggaca
     3181 aaccatatga cgcaagaacg tttacccgaa tttttcgacc gcgccccgac gctgaccgta
     3241 caagacccgc ttgccgcatt cctcggcgcg gccgaaaacg gcatcctcac ttaccgctac
     3301 gccgatgccg tgcgcctgtg cggacattcc tgcccgaccg tcgcgggcgc gtacctgatg
     3361 gttatcaaag gtctgaaagc cctttacgga gaagagctgc ccgaacgcgg cggcatcgaa
     3421 gccgccatgc agggagcgcg cgacgaaggc acggtcggcg taaccgcgtc cgtcgtccaa
     3481 cttctcaccg gcgcagcccc cgaaaccggc ttcggaggca tcggaatgca gggacgcttc
     3541 gcccgccgca acctcttatc ctttggtgta ggcgaaatca acggcacact gaccctgcgc
     3601 cgcaaagaca acggcaaaac cgtcgccgtc ggcctcaacg ccgccctgca acccttcgca
     3661 cccgaaatgc gcgacatcat gcccaaagcc gtcagcggca gcgcaagcgc agaagaactc
     3721 gaacgcttcg gacaactctg gcaggcacgc gttaaagcat ttttaaccga atcggcggac
     3781 gacccgcagt tcgtcatcgt ccgcgaagtg tgagcgttca gacggcattc cgaatttcaa
     3841 atgccgtctg aaccccgcca aacaacaaca aacctacgcc cgacaagcat ccgccatgat
     3901 taccatccgc aacgtcagct accgcatcgg cacacgcccc atcctcgaca acgtcagcct
     3961 cgacatcccc gaaggcggca ttaccgccct cgtcggcccc aacggtgcgg gcaaatccac
     4021 cctgttttcc tttatggcgc ggctgcgacc gcttgaaagc ggcagcatcg cctaccgagg
     4081 caaaaatctt gccgataccc ccaccgccga actcgccaaa accctgtcca tcctcaccca
     4141 agaaaacagc atcatgagcc gcatcaccgt gcgcgacctg ctgatgttcg gccgttaccc
     4201 ctaccatcaa ggcagaccga ctgccgaatg ccgccgtatc gttaacggtg caatcgaaga
     4261 attccacctg caagacctct ccgaccgcta cctgaccgag ctttccggcg gccaacgcca
     4321 acgcgccatg attgcgatgg tgttctgcca aagcaccgac tacgtccttt tggacgaacc
     4381 gctgaacaac ctcgatatgt atcacgcccg ctcgctcatg caaatcctgc gccggctgac
     4441 cgacgaacac aagcgcacca ccgtcgtcgt attgcacgac atcaaccagg cagcagccta
     4501 cgccgaccac gtcgtcgcca tgaaaaacgg ccaagtcgcc atgcagggca aacccaacga
     4561 tattttcacc gccgcaaaca tcaaaaccct attcgatatg gacgtcgacg tcctcgatta
     4621 cgaaggcaaa aaattggtta tccaccatat ctaaatccga caaaaaggcc gtctgaacat
     4681 tcagacggca acccatatcc tgacaaaatt aagacacgac accggcagaa ttgacatcag
     4741 cataatatgc acatattaac agatattaat gccgaactac ctaactgcaa gaattaaata
     4801 aataaataaa taaataaatt gcgacaatgt attgtatata tgcctccttt catatatact
     4861 ttaatatgta aacaaacttg gtggggataa aatacttaca aaagatttcc gccccatttt
     4921 ttatccactc acaaaggtaa tgagcatgaa acactttcca tccaaagtac tgaccacagc
     4981 catccttgcc actttctgta gcggcgcact ggcagccaca aacgacgacg atgttaaaaa
     5041 agctgccact gtggccattg ctgctgccta caacaatggc caagaaatca acggtttcaa
     5101 agctggagag accatctacg acattgatga agacggcaca attaccaaaa aagacgcaac
     5161 tgcagccgat gttgaagccg acgactttaa aggtctgggt ctgaaaaaag tcgtgactaa
     5221 cctgaccaaa accgtcaatg aaaacaaaca aaacgtcgat gccaaagtaa aagctgcaga
     5281 atctgaaata gaaaagttaa caaccaagtt agcagacact gatgccgctt tagcagatac
     5341 tgatgccgct ctggatgcaa ccaccaacgc cttgaataaa ttgggagaaa atataacgac
     5401 atttgctgaa gagactaaga caaatatcgt aaaaattgat gaaaaattag aagccgtggc
     5461 tgataccgtc gacaagcatg ccgaagcatt caacgatatc gccgattcat tggatgaaac
     5521 caacactaag gcagacgaag ccgtcaaaac cgccaatgaa gccaaacaga cggccgaaga
     5581 aaccaaacaa aacgtcgatg ccaaagtaaa agctgcagaa actgcagcag gcaaagccga
     5641 agctgccgct ggcacagcta atactgcagc cgacaaggcc gaagctgtcg ctgcaaaagt
     5701 taccgacatc aaagctgata tcgctacgaa caaagataat attgctaaaa aagcaaacag
     5761 tgccgacgtg tacaccagag aagagtctga cagcaaattt gtcagaattg atggtctgaa
     5821 cgctactacc gaaaaattgg acacacgctt ggcttctgcc gaaaaatcca ttaccgaaca
     5881 cggtacgcgc ctgaacggtt tggatagaac agtgtcagac ctgcgtaaag aaacccgcca
     5941 aggccttgca gaacaagccg cgctctccgg tctgttccaa ccttacaacg tgggtcggtt
     6001 caatgtaacg gctgcagtcg gcggctacaa atccgaatcg gcagtcgcca tcggtaccgg
     6061 cttccgcttt accgaaaact ttgccgccaa agcaggcgtg gcagtcggca cttcgtccgg
     6121 ttcttccgca gcctaccatg tcggcgtcaa ttacgagtgg taagcagcat ctcccgataa
     6181 agaaaccgca gccctgcaag gctgcggttt ttattttcta tccggccgtc aaaccgccgc
     6241 gtccgaacgt tcgcccgtgc ggatacggat tgcctcctcg accggcagca caaaaatctt
     6301 gccgtcgccg atttttcccg aacgcgccac ctcgacaatc acgtcaatcg cgcgttccac
     6361 agcatcatcc gccaacacca gctcgatttt gaccttgggc aggaaatcga cagcgtattc
     6421 cgcgccccgg taaatctccg tatgcccctt ctgcctgccg aaccctttga cctcgctgac
     6481 ggtcataccc gtaatgccga tttccgtcaa cgcctcgcgc acgtcgtcga gtttgaacgg
     6541 tttgacaatc gcctcgattt ttttcataaa atttcctttg aacaaacaat acaaacacat
     6601 ccgaaaaacg ggaacctccc gtcagattgt caacatttta agccaaaata cccaagcaat
     6661 acagccccgt tgcgcgtata atgacagatt ttccaaccgc atttgagagc cgaatccatg
     6721 tctgtcgttt tgcccttgcg cggcgttacc gccctttccg atttccgtgt cgaaaaactc
     6781 ttgcaaaaag ccgccgcact cggactgccc gaagtcaaat taagcagcga attttggtat
     6841 ttcgccggca gcgagaaagc attggatgcc gcgactgtcg aaaaactgca agccttgttg
     6901 gcggcgcaaa gcgttgaaca aacgccaaaa gcgcgcgagg gcttgcattt gtttttggta
     6961 acgccccgtt tgggtacgat ttcgccgtgg gcttccaagg cgaccaatat cgcggaaaac
     7021 tgcggtttgg caggcatcga gcgcatcgag cgcggcatgg cggtgtggct ggaaggtgcg
     7081 cttaccgatg aacagaaaca gcaatgggcg gcgttgctgc acgaccgcat gaccgaatcc
     7141 gtgctgcccg attttcagac ggcatccaaa ttattccacc atctcgaatc cgaaaccttt
     7201 tccaccgttg atgttttagg cggcggtaaa gaggctttgg tcaaagccaa taccgaaatg
     7261 ggtttggcac tctccgccga cgaaatcgat tatctggttg aaaactatca ggctttgcaa
     7321 cgcaatccgt ccgatgtgga gctgatgatg ttcgcgcagg caaacagcga acactgccgc
     7381 cacaaaatct tcaacgccga cttcatcctc aacggcgaaa aacaaccgaa atcccttttc
     7441 ggcatgatac gcgacacgca caacgcgcat cccgaaggca cggtcgtcgc ttataaagac
     7501 aattcgtccg tgatcgaagg cgcgaaaatc gagcgtttct atccgaatgc ggcggaaaac
     7561 caaggctacc gtttccacga ggaagacacg catatcatca tgaaagtgga aacgcacaac
     7621 cacccgaccg ccatcgcgcc gtttgcaggc gcggcaacgg gcgcgggcgg cgaaatccgc
     7681 gacgaaggcg caacgggcaa aggttcgcgt ccgaaagcag gcttgaccgg ctttaccgtg
     7741 tccaacctca acatccccgg cctcaaacag ccgtgggaac aagattacgg caagccggaa
     7801 catatttcct cgccgctgga catcatgatt gaaggcccga tcggcggcgc ggcgttcaac
     7861 aacgaattcg gccgccccaa cctcttgggc tacttccgca cttttgaaga aaaatttgac
     7921 ggtcaggttc gcggctacca caaaccgatt atgattgccg gcggcttggg cagcattcag
     7981 gcgcagcaga cgcataaaga cgaaatcccc gaaggcgcgt tgctgatcca actgggcggc
     8041 ccgggcatgc ttatcggctt gggcggcggc gcggcttctt cgatggatac cggcacgaac
     8101 gacgcttctt tagacttcaa ctccgtacaa cgcggcaacc ccgaaatcga acgccgcgcg
     8161 caggaagtca tcgaccgctg ctggcagctc ggcgacaaaa acccgattat ctccatccac
     8221 gacgtgggcg caggaggttt gtccaacgcc ttccccgaac tggtcaacga tgccggacgc
     8281 ggcgcggtat tcgagctgcg cgaagtgccg ttggaagaac acggtttaac gcctttgcaa
     8341 atctggtgca acgaatcgca agagcgttat gtgttgtcga ttttggaaaa agatttggat
     8401 accttccgcg ccatctgcga acgcgaacgc tgcccgtttg ccgtggtcgg tacagcgacc
     8461 gacgacggtc atttgaaagt gcgcgacgat ttgttcgcca acaaccccat cgatttgccg
     8521 ttgaacgtct tgctcggcaa accgcccaaa accacgcgta ccgacaaaac agttacgcca
     8581 tctaaaaaac cgtttcacgc cgacgatatc gacatcaccg aagccgccta ccgcgttttg
     8641 cacctgcctg ccgtagccgc caaaaacttc ctgattacca tcggcgaccg cagcgtcggc
     8701 ggcatgaccc accgcgacca aatggtcggc aaataccaaa cccccgtagc cgactgcgcc
     8761 gttaccatga tgggcttcaa tacctatcgc ggcgaagcaa tgtctatggg cgaaaaaccg
     8821 accgtcgccc tgtttgacgc gcctgcttcg ggcagaatgt gcgtcggcga agccatcacc
     8881 aacatcgcgg cggtcaacat cggcgacatc ggcaacatca agctctccgc caactggatg
     8941 gcggcgtgcg gcaacgaagg cgaagacgaa aaactctacc gcaccgtcga agcggtttcc
     9001 aaagcctgtc aggcattgga tttgagcatt cccgtgggca aagacagcct gtcgatgaaa
     9061 accgtatggc aggatggcga agagaaaaaa tccgtcgttt cgccgttgag cctgattatc
     9121 tccgcgttcg cgccggttca agacgtacgc aaaaccgtta cgcccgaatt gaaaaacgtc
     9181 gaggacagcg tattgctgtt tgtcgatttg ggcttcggca aagcgcgtat gggcggctct
     9241 gcattcggtc aggtatacaa caacatgagc ggcgacgcgc ccgatttgga cgacacaggc
     9301 cgtctgaaag cgttttacaa cgtgattcaa cagcttgtcg ccgaagacaa actcttggca
     9361 taccacgacc gcagcgacgg cggcttgttt gccgttttgg tagaaatggc gtttgcaggg
     9421 cggtgcggct tggatataga tttaaattta ttgcttgcac aaacatttat taccaaccat
     9481 accgctctgt ctcaatcatt gcggactgaa gaggtaaaag cgttggctga atggcaagaa
     9541 accattgccc gcacattatt taatgaagag ttgggtgctg ttatccaagt tagaaaacaa
     9601 gatgttgccg atattatcaa tttattctat caacaacagc tgcatcataa tgtctttgaa
     9661 atcggtacgt taactgatga gaacacgtta atcatccgcg acgggcaaac gcaccttatt
     9721 tctgacaacc taatcaaact gcaacaaacc tggcaagaaa ccagccatca aatccaacgc
     9781 ctgcgcgaca accctgcctg cgccgacagc gagtttgccc tgattggcga caacgaccgc
     9841 agcgcattgt ttgccaacct gaaattcgac gtgaacgaag acatcgccgc gccgtttatc
     9901 aacagcggcg cgaaacccaa aatcgccatc ctgcgcgaac agggcgtgaa cgggcaaatc
     9961 gaaatggccg ccgccttcac ccgcgccgga ttcgatgctt acgacgtgca tatgtccgac
    10021 ctgatggcag gccgcttccg ccttgccgac ttcaaaatgc tggcggcgtg cggcggcttc
    10081 agctacggcg acgtactcgg cgcgggcgaa ggctgggcga aatccatcct gttccaccct
    10141 gccttgcgcg accaatttgc cgccttcttt gccgatccgg acacgctgac attgggcgtg
    10201 tgcaacggct gccagatggt cagcaacctc gccgagatta tcccgggtgc agaggcatgg
    10261 cctaaattca aacgcaacct gagcgaacag tttgaagcac gcctgagcat ggttcacgtt
    10321 ccgaaatcag cgtcgctgat tctgaacgaa atgcaaggct ccagcctgcc tgtcgtggtc
    10381 agccacggcg aaggccgcgc cgacttcgcg cttcacggcg gcaatatttc cgccgatttg
    10441 ggcatcgcgc tgcaatatgt ggacggtcaa aaccaagtga cccaaaccta cccgctcaac
    10501 cccaacggct cgccgcaagg catcgccggc gttaccaacg ccgacggccg cgtgaccatc
    10561 atgatgccgc atccggaacg cgtgtaccgt gccgcacaaa tgagctggaa accagaagac
    10621 tggacggaat tgtccggctg gtaccgcctc tttgccggcg cacgtaaagc cttgggctaa
    10681 ccgccctact caaaccaatg ccgtctgaag aatatttcag acggcgttcc ggcataccat
    10741 cctttaaacg gtatccgtcc accgaggaac actcatgaaa atcacccccg tcaaagccct
    10801 aaccgacaac tacatctgga tgatacagca cggcaaccat gccgtctgcg tcgacccttc
    10861 cgaaccctcg cccgtcttgg aattcctcgt ccgcaaccgc ctcatgcttg cccaaacatg
    10921 ggtaactcac ccccaccccg accacgaggg cggtgcggcg gcactctggc gcggctacat
    10981 ggaatcgccc gtttacggcg aatccgacat cgaagcagca acccacaccg taaccgccgg
    11041 cacccaattc accttcggcg acggacaggt taccgtttgg gcaacacccg gccacacaga
    11101 ccgccacacc agctaccttc tcgaaacttc agacggcata cacgttttct gcggcgacac
    11161 cctgttttcc gccggctgcg gacgcgtgtt taccggcaca atcgaacagc tttacgacag
    11221 cttccaacgc ttcaaccgcc tgcctgaaaa caccctgttc tacccggcac acgaatacac
    11281 cgccgccaac ctggattttg ccgcccatat cgagccggac aacgccgaca ttcagacggc
    11341 actgaaagcg gcggcgcata cgcctaccct gcccgttacc ctcgcgcacg aacgccgcgt
    11401 caatccgttt ttgcgcgtcg acctgccgca cgtcagagac cgcgccgagg cattgagcgg
    11461 gaaaacgtta aacagcagcc tcgatacctt tgtcgcgctg cgtgaactta aaaaccaata
    11521 ccggacgaaa taaaacaacg ggaaaacgca gccattccta ggatttttat taaaatctta
    11581 aataaaatca tacaatcatc gccaatagac gaaaggacac cgttgcctta taatcaaaca
    11641 aaaacaaaat atataatata gtggattgaa tttaaatcag gacaaggcga cgaagccgca
    11701 gatagtacgg caaggcgagg caacgctgta ctggtttttg ttaatccact atattgttaa
    11761 tccactatat aaatccagca caaaacggga tcggtgattc ttgtccgcaa gaatcgttga
    11821 ttttctctat tacacggata atcatcatgc gcttcacaca caccacccca ttttgttccg
    11881 tattgtccac cctcggtctt tttgccgttt cccctgctta ctcatccatt gtccgcaacg
    11941 atgtcgatta ccaatatttt cgcgactttg ccgaaaataa aggcgcgttc gccgtaggtg
    12001 caagcaatat ttccatccaa gacaagcaag gcaaaatatt aggcagggtt ctcaacggca
    12061 tccccatgcc cgacttccga gtcagcaacc ggcaaaccgc catcgccacc ctgattcacc
    12121 cccaatacgt caacagtgtc aaacacaacg tcggctacgg ttccatacaa ttcggcaacg
    12181 acacccaaaa tccagaagaa caagcctata cctaccgcct cgtatcacgc aacccgcacc
    12241 cggactacga ctaccacctt ccccgcctca acaaactggt taccgaaatc tcacctgccg
    12301 cactcagcag cgtacccttg cttggaaacg gccagccaaa ggccaatgcc tacctcgata
    12361 ccgaccgctt cccctacttt gtacgactcg gctcaggcac gcaacaagtc cgcaaagcag
    12421 acggcacgcg tacacgaacc gccccggcat accaatacct gaccggcggc acgccgctga
    12481 aagtattggg gttccaaaac cacggcttac tcgtcggcgg cagcctgacc gaccaacccc
    12541 ttaacaccta cgcaatcgcc ggagacagcg gttcccccct gtttgccttc gacaagcatg
    12601 aaaaccgctg ggtgcttgcg ggcgtactca gcacctacgc cggcttcgat aatttcttca
    12661 acaaatacat cgtcacgcaa cccgaattca tccgttccac catccgccaa tacgaaaccc
    12721 ggctggatgt cgggctgacc accaacgaac tcatatggcg cgacaacggt aatggcgaca
    12781 gcaccctgca agggctcaac gaacgcatca ccctgcccat tgcaaaccct tcgcttgccc
    12841 cacaaaacga cagcaggcac atgccgtctg aagatgccgg caaaacgctc atcctatcca
    12901 gcaggttcga caacaaaaca ctgatgctgg cagacaatat caaccaaggc gcaggcgcat
    12961 tgcagttcga cagcaacttc accgtcgtcg gtaaaaacca cacatggcaa ggtgcaggcg
    13021 ttatcgtagc cgacggcaaa cgcgtcttct ggcaagtcag caaccccaaa ggcgaccggc
    13081 tctccaaact gggcgcaggc acgcttatcg ccaacggaca aggcatcaac cagggcgaca
    13141 tcagcatcgg ggaaggcact gtcgtactcg cccaaaaagc tgcttcagac ggcagcaaac
    13201 aagcattcaa ccaagtcggc atcaccagcg gcaggggtac ggccgtcctc gccgacagcc
    13261 agcaaatcaa atccgaaaac ctctatttcg gcttcagggg cggacggctc gacctcaacg
    13321 gcaacaacct tgcctttacc catatccgcc atgcggacgg cggcgcgcag atcgtcaacc
    13381 acaaccctga ccaagccgcg acactgacgc tgaccggcaa ccccgtcctc agtcctgagc
    13441 atgtcgagtg ggtgcaatgg ggcaaccgtc cgcaaggcaa cgcggcggtt tacgaataca
    13501 tcaacccgca ccgcaaccgt cggaccgact acttcatact caaacccggc ggcaacccgc
    13561 gcgaattttt cccgttaaat atggaaaact caacaagctg gcaatttatc ggcaacaaca
    13621 ggcaacaggc cgccaaacaa gtcgcccaag ccgaaaatgc ccgccccgac ctaattacct
    13681 tcggcggata cttgggtgag aacgcgcaaa cgggcaaagc cgcaccgagt tacagcaaaa
    13741 ccaatatcga atgggtgaat ggtccaaaca ccccacacaa gccctcgccc tatacgaata
    13801 cgccaacccg cacaggggca ggcgtaccga cgattaccgc ctcaaagaag gcggcaaccc
    13861 cggcgcctac ttcccatgga atgcctacag cacccaagac tgggaatttc tcggacatga
    13921 ccggcgaagc agccatagaa aaaaaccgcc atatcgcaaa tgccgccgta tacggccggc
    13981 ccgaataccg ttacaacggc gcactcaacc tgcactatcg tcccaaacgc accgacagca
    14041 cgctgttgct caacggcggc atgaacctta acggggaagt cttgattgag ggcggcaata
    14101 tgattgtgtc aggcaggccc gtaccccatg cctacgacca ccaggccaaa cgcgaacccg
    14161 ttcttgaaaa cgaatggacc gacggcagct tcaaggctgc acggttcacc ctacgaaacc
    14221 atgcccgact gacagcaggg cgcaataccg cgcatctgga cggcgacata accgcataca
    14281 atctgtccgg catcgacctc ggctttatcc aaggcaaaac accggaatgc taccgctcct
    14341 accatagcgg cagcacccac tgcacaccca acgccgtttt aaaagccgaa aactatcgtg
    14401 cactacctgt aacgcaagta cgcggcgaca ttacccttaa cgaccgttca gagctccgcc
    14461 tgggcaaagc acacctgtac ggcagcatcc gtgccggcaa agacaccgca gtccgcatgg
    14521 aagcagacag caactggaca ctttcccagt ccagccacac cggcgcactg acgcttgacg
    14581 gcgcacaaat taccctgaac cccgatttcg ccaataatac acacaacaac cgcttcaaca
    14641 cactgaccgt caacggcaca cttgacgggt tcggcacatt ccgattcctg accggcatcg
    14701 tccgaaaaca aaatgccccc ccccctcaaa ctggaagggg acagccgcgg cgcattccaa
    14761 atccacgtca aaaacaccgg acaagaacct caaacaaccg aatcgcttgc acttgtgagc
    14821 ctcaatccga aacacagcca ccaagcccga ttcaccctcc aaaacggcta tgccgatttg
    14881 ggtgcctacc gctacatcct ccgcaaaaac aacaacggat acagcctgta caacccgctc
    14941 aaagaggccg aacttcaaat tgaagccacg cgtgcggaac atgagcgcaa ccaacaggca
    15001 tacaaccaat tacaggcaac cgacatcagc agacaggttc aacatgactc tgacgcgacc
    15061 aggtaggcac tacaggcctg gcagaacagt caaaccgaac ttgcccgcat cgacagccaa
    15121 gtccaatatc tgtccgccca attgaaacag acagacccgc tgaccggcat tctgacgcgt
    15181 gcccaaaacc tgtgtgccgc acaaggatac agtgtcgata tctgccgtca ggttgccaaa
    15241 gccgccgaca cgaacgacct gacactcttc gaaaccgaac tggatacgta tatagaacgt
    15301 gtagaaatgg ccgaatccga acttgacaaa gcacggcaag gcggcgatgc gcaagccgtc
    15361 gaaacagccc ggcacgccta cctgaacgca ctcaaccgtc tgtcccgaca aatccacagt
    15421 ttgaaaaccg gcgttgccgg catccgtatg ccgaacctgg ccgaactgat cagccggtcg
    15481 gccaacaccg ccgtttccga acaggccgcc tacaataccg gccggcaaca ggcgggacgc
    15541 cgcatcgacc gccaccttac cgatccgcag cagcaaaaca tctggctgga aaccggtacg
    15601 caacaaaccg actaccatag cggcacacac cgtccctacc aacaaactac caactatgca
    15661 catatcggca tccaaaccgg catcaccgac cgtctcagtg tcggtacgat tttaaccgat
    15721 gagcgcacaa acaaccgttt tgatgaaggc gtatccgccc gaaaccgcag caacggcgca
    15781 catctgttcg tcaaagggga aaacggcgca ctctttgccg cggcagattt aggctacagc
    15841 aacagccgta cccgatttac cgattatgac ggggctgccg tccgccgcca cgcatgggat
    15901 gcaggcatca acaccggcat caaaatcgat accggcatca acctcagacc ctatgccggc
    15961 atccgtataa accgcagcaa cggcaaccgg tacgtactcg acggcgcaga gataaacagc
    16021 ccggcgcaaa tccaaaccac atggcatgcc ggcatccgtc tcgataaaac cgtcgaactg
    16081 ggtcaagcca agctgacccc cgctttcagc agcgattact accatacccg ccaaaacagc
    16141 ggttccgccc tcagcgtcaa cgaccgtacc ttactgcagc aagccgccca cggcacactg
    16201 cataccctgc aaatcgacgc cggatacaaa ggctggaacg ccaaacttca tgccgcttac
    16261 ggcaaagaca gcaacaccgc ccgccacaaa caggcaggaa tcaaaatagg ctacaactgg
    16321 taacaagccg ataaaaatgc cgtctggaac ccgcgtttca gacggcattt gcgttaaaaa
    16381 tagtaaaccg ttccaaaagg gagtagaata gtgccgtttc caaccctgcg cctgtaccgt
    16441 caggctttta ttatggacct tcccagttcg tttttactga acgccccttc cgattccaaa
    16501 cgacaataac catcccgccg gaatgcctcc ccgcacacga cgggcggagc atttatgagc
    16561 atcgaacaca ctcctccgac acacgacggc gaaaccggtc aaaaccatgc cgaacgccct
    16621 tccgctgatt tcgaccgtgt ccactccctc tgcgaaatcc tcgaacctgc ctttgaacaa
    16681 atcgaaaacg gcacaccgct cgaagacgcg ccgctgcgcg acaagctgac cgagctgacc
    16741 gtcctcttga gcgagctgca ccctgccgac gtggcggcag tgttggaatc gctgccgccg
    16801 cgcgaacgca atatcgtctg gattctggtc aaaccggaag acgacggcga agtcttgctg
    16861 gaagtgtccg atgcggtgcg cgaaacgctg atcgagtcga tggacaaaga cgaattgttg
    16921 gcggcggtcg atgatttgga cgcggacgaa ttggcggagc tggcagacga tttgccgcac
    16981 caagtggttt atgaagcctt gcagacgcgc gacgaggaag agcgcgcaca agtcaaagcg
    17041 gcaatgtcct acgaagacaa ccaagtcggt gcgattatgg acttcgagtt ggtcagcatc
    17101 cgcgccgatg tcgcctgtga agtggtgctg cgctatctgc gccgcttcga cagcctgccc
    17161 gaccataccg acaagatttt tgtggtcgat gaaaacgacg tactgcaagg cgtgctgccc
    17221 atccgcaaac ttttggtcgc cgatcccgaa gatttggtgg aaaacgtgat ggcgaaagat
    17281 gtcgtgcgtt tccgcgccga agacgacgtg gaagaagcag cgcaggcatt cgaacgctat
    17341 gacttggtta ccgcgcccgt cgtcgatgaa aacaaaaagc tcatcggcag ggtgaccgtt
    17401 gacgaaatgg tggacgtgat ccgcgaagaa tccgaagcgg atatgttgaa catggcaggt
    17461 ttgcaggaag aggaagacct gttcgccccc gtcttggatt ccgtcaaaaa ccgctggatg
    17521 tggctcgccg tcaacctctg caccgccttc ctcgccagcc gcgtcatcgg cgcgtttgaa
    17581 ggcagcatcg aaaaaatcgt cgcactcgcc gcgctgatgc ccatcgtcgc cggcatcggc
    17641 ggcaactcgg gcaaccagac gattaccatg attgtccgcg cgatggcgat ggggcaactg
    17701 acaggcatgc aggcaggcag gctgttgaaa aaagaagtcg gtgtcgcctt ggtcaacggc
    17761 atcatttggg gaacggtcat gggcgcagta tcttggctgc tttacggcag cctcggcatc
    17821 gggctggtta tgattgccgc gatgacgctc aacctcctgc tggcggcaac cgtcggcgta
    17881 ttaattcccg tggtaatgga aaagttcgga cgcgatcccg cactgggcag ctcggtgctg
    17941 attaccgccg ttaccgactc cggcggcttc ctgattttct tggggctcgc caccctattc
    18001 ctgctttaaa tgccgtctga acccgcgcaa aaatgccgtc tgaagcggaa gctgcttcag
    18061 acggcatttg actatttatc cttgttgcac aagattattg gacggtatgc cggggcagcc
    18121 ctttggcaac gccgaccaca tcctccccga acagcgcgtt gacatcggtt tcgtcaaaca
    18181 catatttgct gtggcagaaa tcgcaatcga cttcgatgct gccttgttcc accaccacgc
    18241 cgccgacttc ttccccgccc agcatcaaca gcatatcgct gactttgccg cgcgaacagg
    18301 tgcatgaaaa ttcaaacgtt tccggctcga acacgcgcgg cggcgtttcg tggaacaagc
    18361 ggtacaaaac atgttgcgca tccaattctg ccaactcgcc cgccgtcagc gtgcgcgcca
    18421 gcgtactgac gtgttcccat gcctcttcat ccaacacctc ttcaggcaga cgctgcacca
    18481 gcagaccgcc cgccgcttca tcgcttgcag acaggacgat gtgcgtatca agctgttcgg
    18541 aacgtttcat atagttcatc aacatttgcg cgataccgtc gccttccaaa ggcactacgc
    18601 cctgccaggg ttcgccgtct ttgggctgca gcgtcagcac gaatacgccg ccctcgccca
    18661 aaagatcgac caaactttca tcttcaccga tttctgcagc ctcgtcccac cgcgcggtcg
    18721 cacggacggt acggtcggaa gtcgcttccg caaccagcat tttcagccgc ccctgcccct
    18781 gaacctgcac aatcagcgtg ccttcgtttt tgaggttgcc cgacagcaga acaccggcag
    18841 ccaacagctc gcccaaggcg cggcggatgg cggcgggata gtttttctgt tttacaatgt
    18901 gctgccactc gttttccaga cggacgtgca gcccgcgcac gggcatatcg tcgaagataa
    18961 agcgggtacg cacatcggcg cggttgatgg cggtttgatt catgattttc tctgactgat
    19021 tgttcggatg gcggctatat ggttgcggtc ggcgcgaaaa caagacggac ggcggatgcg
    19081 cttcccaaat tatcaataaa ttatataaaa atcaacatat taactcaatc taacaagccg
    19141 ttttttgcca aacagccgtt tttttatata caatcaacaa gatattttcg actgatacag
    19201 cataacatcg cacggcggca cgatgcctcc tgcgcggaaa caccgatatg gattcttttt
    19261 tcaaaccggc agtttgggcg gttttgtggc tgatgtttgc cgtccgcccc gcccttgccg
    19321 acgagttgac caacctgctc agcagccgcg agcagattct cagacagttt gccgaagacg
    19381 aacagcccgt tttacccatc aaccgagccc ccgcccggcg ggcgggcaat gccgacgaac
    19441 tcatcggcag cgcgatgggg cttaacgaac agcccgtttt acccgtcaac cgagtccccg
    19501 cccggcgggc gggcaatgcc gacgaactca tcggcaacgc gatggggctt aacgaacagc
    19561 ccgttttacc cgtcaaccga gcccccgccc ggcgggcggg caatgccgac gaactcatcg
    19621 gcaacgcgat gggacttttg ggtattgcct accgctacgg cggcacatcg atttctaccg
    19681 gttttgactg cagcggcttc atgcagcaca tcttcaaacg cgccatgggc atcaacctgc
    19741 cgcgcacgtc ggcagaacag gcgcggatgg gtacgccggt tgcccgaagc gaattgcagc
    19801 ccggggatat ggtgtttttc cgcacgctcg gcggcagccg catttcccat gtcggacttt
    19861 atatcggcaa caaccgcttc atccacgcgc cgcgcacggg gaaaaatatc gaaatcacca
    19921 gcctgagcca caaatattgg agcggcaaat acgcgttcgc ccgccgggtc aagaaaaacg
    19981 acccgtcccg ctttctgaac tgatttccca aggaatacgc aatgagtatg cccgaaatgc
    20041 ccaaatggta cgacgatgac ggacagatcg tgtcctgtac cgaaaaggtc aaagtgatgt
    20101 ccgaaaatat ggccgagctg tatcagacgg cacaagacgc gtttgaagac gcgctgctga
    20161 tgggttgcgg cgaacgtcag ttgcgcgatt acctgctcgc gctgattgaa ggtttggaaa
    20221 atccctaccg caaagtctga acacgccccg gttgctgcgg cacggtttat ccgtgccgtt
    20281 tttgcgtttg tgcgcggctt cggcttttca gacggcatat ttgacgttat gattaaacag
    20341 ttaacaagat ttatcacaac gccgtcaaaa gacagacaca caacatgaac atcatccgcg
    20401 cgctcctcat catcctcggc tgcctcgccg ccggcgaaac cgccgttttc ctagcaggca
    20461 tcaaactgcc cgacagcatc gtcggcatgg gcgtgctgtt tgcgcttttg caggcaggtt
    20521 gggtcaaaac gtcttggctg caacagctta ccgacgcgct gatggcgaat ctgacgctgt
    20581 ttctcgtgcc gccctgcgtg gcggtcatca gctatttgga tttgattgcc gacgattggt
    20641 tttcgatact ggtttccgcc tccgccagca ctttgtgcgt actgctggtt acaggcaagg
    20701 ttcaccgctg gatacggagc attatctgat gaacgaaatc ctcaggcagc ccagcgttct
    20761 gcttttcctc acgcttgccg tgtacgcgct tgcgattatc gtgcgcacgc gcacgggcaa
    20821 tatcttctgc aaccccgtac tcgtcagcac tatcgtgctg attgcctacc tgaaaatcct
    20881 cgacatcgat tatgcggtgt accacaacgc cgcgcaattc attgattttt ggctgaaacc
    20941 cgccgtcatc gtgcttgccg tgccgctcta ccaaaaccgc cgtaaaatct tcaaccagtg
    21001 gctgcccgtc atcgtttcac agcttgcggg cagcgttacg ggcattgtta cggggatgta
    21061 ttttgccaaa tggctgggcg cggaacgcga agtcgtcctc tcgctcgcgt ccaaatctgt
    21121 taccaacccc atcgccattg aaatcacccg ctccatcggc ggcattcccg ccattaccgc
    21181 cgccaccgtc atcattgccg gtctggtcgg acagattgcc ggttacaaaa tgctgaagaa
    21241 cacagtcgtc atgccctcgt ccgtgggtat gtcgctcggc acggcttcgc acgcgatggg
    21301 gattgccgcc tcgctcgaac gcagccgccg catggcggca tacgcgggac tggggctgac
    21361 gttcaacggc gtactgaccg cgctgattgc gccgctgctc atccccgttt tgggattttg
    21421 aacccgtttc agacggcatt tcagcccatg ctgtctgaac gccgacacac gcgcaaggag
    21481 aaccgttatg gctgtcaacc tgactgaaaa aaccgccgaa caactgcccg acatcgacgg
    21541 cattgccctc tacaccgccc aagcaggcgt gaagaagccc gggcataccg acctgacact
    21601 gattgccgta gccgccggca gcaccgtcgg tgcagtcttc acgaccaacc gtttctgtgc
    21661 cgcgcccgtc cacatcgcca aatcgcacct tttcgacgaa gacggcgtgc gcgccctcat
    21721 catcaacacg ggcaacgcca acgcgggtac gggcgcacag ggcagaatcg atgctttggc
    21781 agtgtgtgcc gccgccgccc ggcaaatcgg ctgcaaaccg aaccaggtgc tgcccttctc
    21841 caccggcgtg attctcgaac cgctgcccgc agacaaaatc atcgccgcgc tgcccaaaat
    21901 gcagcctgcc ttctggaacg aagcggcacg cgccatcatg accaccgaca ccgtgcccaa
    21961 agccgcctcg cgcgaaggca aggtcggcga caaacacacc gtccgcgcca cgggcatcgc
    22021 caaaggctcg ggcatgattc atcccaatat ggcgaccatg ctcggtttca tcgccaccga
    22081 tgccaaagtt tcccaacccg tcctccaact gatgacgcag gaaatcgccg acgaaacctt
    22141 caacaccatc accgttgacg gcgacaccag caccaacgac agcttcgtca tcatcgccac
    22201 cggcaaaaac agccaaagcg aaatcgacaa catcgccgac ccgcgttacg cccaactcaa
    22261 agaattgttg tgcagcctcg cgctcgaact cgcccaagcc atcgtccgcg acggcgaagg
    22321 tgcgaccaag ttcatcaccg tccgcgtcga aaacgccaaa acccgcgacg aagcccgcca
    22381 agccgcctac gccgtggcac gttcgccgct ggtcaaaacc gcctttttcg cctccgaccc
    22441 caacctcggc aggctgctcg ccgccatcgg ttatgccggc gttgtcgacc tcgatactga
    22501 cctcgtggaa atgtatctcg acgatatttt ggttgccgaa cacggtggac gcgccgcaag
    22561 ctacaccgaa gcacaagggc aggcggtgat gtcgaaggcc gaaatcaccg tccgcatcaa
    22621 gctgcatcgc ggacaaagtg ccgccaccgt ctatacctgc gacctgtcgc acggatacgt
    22681 ttccatcaac gccgactacc gttcctgacc cgacacggct tcagacggca tacataaaat
    22741 gccgtctgaa ccgccggaca acatgccatg acctccacat tcccccgccg cctcgcccgc
    22801 aaaatccgcc aaacccgccg cctgtcgcgc aaaagcatcg cctttctgtt ccttttggca
    22861 ggttcggcac tcgtcgccct gaccgcgctg ttttttgccc atcttgccga ttttgcgctg
    22921 gaactgaacg ccaaactggt tcaacaatac ccgtggttcg cgtgggtcgc gcttcctttg
    22981 ggtttgccgc ttattgcgtg gctcacacgc aaattcgccc ccttcaccgc cggcagcggc
    23041 atcccgcagg tcatcgcctc actgtcgctg ccctacggcg cacagaaaac gcggctgatc
    23101 cgcctcgggc agacgctgct gaagattccg ctgacctttt tgggtatgct gttcggcgtg
    23161 tccatcggac gcgaaggtcc gtccgtgcag gtcggcgcgg cagtgatggg cgcgtggggc
    23221 gcgtggtgca agaaacacgg cttggcattc aaagggatgc aggaaaacga tttgatggcg
    23281 gcgggcgcgg cgggcggttt ggcagccgcg ttcaacgcgc cgctggcggg cgtgattttc
    23341 gccattgagg aactcgggcg cggcatcatg ttgcgctggg agaggcaaat tcttttgggc
    23401 gtgctcgcct ccggtttcat acaggtcgcc attcagggca acaacccgta tttttccggc
    23461 ttcaacggcg gcgtattgga acatatcttt ctgtgggtcg cactgtccgg cctggtttgc
    23521 ggcgcggcgg gcggcctgtt cggacgtttg ctctatcgcg gtgcggcggc gtttgcaccg
    23581 cgcaagatac gcggcttcat ccgcaaccgt ccgctgctgc tggcggcact gatggggctg
    23641 ctgctcgccc tgctcggcac gttctaccaa ggcaaaacct acggcaccgg ctaccacgaa
    23701 gccgcccaag ccctgcacgg catctacgaa gcccccttcg gactcgccgc cgccaaatgg
    23761 ctcgccaccg tattcagcta ttgggcaggc gttccgggcg gcattttcac tccctcgctg
    23821 accataggcg cggttttggg cgagcatatc gccgccatcg ccgacatatc gcagggtgca
    23881 aacatcatcg tcctcatctg catggcggca tttctggcgg gcgcgacaca atccccgatt
    23941 acttccgccg tcgtcgtcat ggaaatgacg ggcggacaaa gcctgctgtt ttggatgcta
    24001 attgcctgca ttttcgcctc gcaggtttcg cgccagtttt cgccgcgtcc gttctaccac
    24061 gcatcgggaa tgcgcttccg ccagcgcgtg cttcaagaaa ccgccgccca aaccggcaat
    24121 gcgcccgcaa gaccgcaaac agcaaacagc aaaacgggaa tgccgtctga aaattaaaac
    24181 gcccccgatc aaacgccggc agccgccttg atttgaatac cgttccgccg ccgcttgaaa
    24241 tttcagcaac aatgccgtct gaacgacaga atgcggtttt cagacggcat ttccccatcc
    24301 cgatattgcc taaacaaaac cgaagcgttt gctataattg cactttttac cgcacacgca
    24361 cgcccatgtt tcccgatttc tcccaaaccc tctccaaaga ccgccacttc ctgcaatccg
    24421 ccttcaaaaa tcccaacaaa tacggcggct tgtccaaagt cgaagaaaaa taccgaaaat
    24481 cgcacgaaat ctttttgaaa cgtttggcag ccttgcccaa acccgaattc gacaacaccc
    24541 tgcccgttca cgaaaaactt gacgaaatca aaaaaaccgt tgccgaacat caggtaacga
    24601 ttatttgcgg cgaaaccggt tcgggcaaaa ccacgcagtt gcccaagatt tgtttggaac
    24661 tcgggcgcgg ggtagcgggt ttgatcgggc atacccagcc gcgccgtttg gcggcgcgtt
    24721 cggtggcaga gcggattgcc gaagagctga aatccgaaat cggcagcgcg gtcggctata
    24781 aagtacgctt caccgaccac acctcgcgcg atgcctgcgt caagctgatg accgacggca
    24841 tcctgctggc ggaaacgcag accgaccgtt atctcgccgc ctacgacacg attatcatcg
    24901 acgaagcgca cgagcgcagc ctgaacatcg acttcctttt gggctatttg aaacaactcc
    24961 tgccgcgccg ccccgatttg aaagtcatca tcacctcggc aacgatagac gcagaacgct
    25021 tctcccgaca cttcaacggc gcgcccgttt tagaagtgag cggacggacg tatcccgtcg
    25081 aaatcctcta ccgaccgctg accggcaaag acgaagacga cgcagaagtg gagttgaccg
    25141 acgcgattgt cgatgcggcg gacgaattag cgcgacacgg cgaaggcgat attttggtat
    25201 tcctgccggg cgagcgcgaa atccgcgaaa ctgccgaagc cttacgcaaa tctacactgc
    25261 gccgcaacga cgaaatcctt cccctgttcg cacgcctgtc gcacgccgag cagcacaaaa
    25321 tcttccaccc ttcaggcgcg aaacgccgca tcgtattggc aaccaacgtc gccgaaacct
    25381 cgcttaccgt gccgggcatc aaatacgtca tcgacaccgg cctcgcgcgc gtcaaacgct
    25441 attccgcacg ggcaaaggtg gagcagcttc atgtcgaaaa aatctctcaa gccgccgccc
    25501 gccaacgctc cggccgctgc ggacgcgtct ccgcaggcgt gtgtatcaga ctgttttcag
    25561 aagaagattt caacagccgc cccgaattta ccgacccaga aatcgtccgc agcaacctcg
    25621 ccgccgtcat cctgcgcatg gcatcgctga acctgggcga tgtggcggca ttcccatttt
    25681 tagaaatgcc cgattcgcgg tatatcaatg acggttttca ggtattgctg gaattggggg
    25741 cggtagaggc cgtctgaaaa caagtaggat atacacttct acctgacaaa gaccaacaaa
    25801 cgataaaact acggcacatc tagatgccca acctacaaca ttgcacacgg taaaaacatg
    25861 aaacacttac tcatcgattt tgaaaacgtc cagccgcaaa acttagacaa attaccaacc
    25921 gaaaataccc atatttggct atttataggt gtattacaca aaatgttacc tattagtctg
    25981 gtgcaatccc tactacgttt cggcgaacgt gtccatcttg tccagttaca aaaaacgggg
    26041 aaaaacgcat tggattttta cctgtcctat tacctcggac aaattaccgc cacagacccc
    26101 aatgcccaaa tcggcatact ctcgcgtgat ggaggatacg atgttctggt cgaacatatt
    26161 ttgaaaaacc accaggcgaa gggtatcgtg cgcctagcca atatagatga agtacaacat
    26221 cagaaaatta ctaccgaacc gccgtcagca ttgctggaaa acactcctca gcctgaaacc
    26281 accctcaaac cacagcaacc attaacttcc tatttccaag cagccctaac tgcactgcgc
    26341 cgccccgacg ctttccgccc ctgccgcctg cataacctgc gacaaaatct gcgtaagcat
    26401 attttgagtg atttgtttaa agaaaaaacc gatgaagaat gcgaaataac cactgctaac
    26461 gttatcaata aactcaaagc acaaaacttc atcagcattg atgaacagga aaccgtttcc
    26521 taccatctca gtgataatga tttgttacaa agaatccaac gccatatttt aagccaacgt
    26581 cccaaaacct acgctgattt tcaagccgtc gtgcaaaacc gagcagatgc acttcactta
    26641 acagtcggta ccaacgacat tcaatccttt gcgcgacatt tgcgcgacca aaacctgatc
    26701 cgccaaaaca atgggaaaat tgaatatgca ccgtttactg aacctaaacc acagccaacg
    26761 cccaagcagc ctaaaaaaac cgcatgggaa cctgatgaaa ttatttggaa aaaagtgatt
    26821 gccgcgttat cgttaaagaa ccgtcctaat aaaaccaaaa ctttacgcaa tacaatccag
    26881 gcactcacaa aatccaatgc acaagaaact gacaaactgc tacaacattt acaagatacc
    26941 caagtcctac gtattgacgg tactaaaatt gtgtatatca agtaggatgg gcatttatgc
    27001 ctgacctaca aaaccaaaaa caacctacca cccttaatca actccacaaa cccttttcag
    27061 acaacctcgt tttttgaaaa acaatctgta aacagatagc tgctgaagaa taccgttgcc
    27121 gagccccaaa acccgtcctg caacttttat tgtgaacttc ccattatgaa aaaatccctt
    27181 ttcgttctct ttctgtattc gtccctactt actgccagcg aaattgctta tcgctttgta
    27241 ttcggaattg aaaccttacc ggctgcaaaa atggcagaaa cgtttgcgct gacatttgtg
    27301 attgctgcgc tgtatctgtt tgcgcgttat aaggcgacgc gtttgttgat tgcggtgttt
    27361 ttcgcgttca gcattattgc caacaatgtg cattacgcgg tttatcaaag ttggatgacg
    27421 ggcatcaatt attggctgat gctgaaagag attaccgaag ttggcggcgc gggggcgtcg
    27481 atgttggata agttgtggct gcctgcgttg tggggcatgt tggaagtcat gttgttttgc
    27541 agccttgcca agttccgccg taagacgcat ttttctgccg atatactgtt tgccttccta
    27601 atgctgatga ttttcgtgcg ttcgttcgac acgaaacaag agcacggtat ttcgcccaaa
    27661 ccgacataca gccgtatcaa agccaattat ttcagcttcg gttattttgt cggacgcgta
    27721 ttgccgtatc agttgtttga tttaagcaaa atccctgtgt tcaaacagtc tgcgccaagc
    27781 aaaatcgggc agggcagtgt tcaaaatatc gtcctgatta tgggcgaaag cgaaagcgcg
    27841 gcgcatttga aattgtttgg ctacgggcgc gaaacttcgc cgtttttgac ccagctttcg
    27901 caagccgatt ttaagccgat tgtgaaacaa agttattccg caggctttat gacggcagta
    27961 tccctgccca gtttctttaa cgtcataccg catgccaacg gcttggaaca aatcagcggc
    28021 ggcgatacca atatgttccg cctcgccaaa gagcagggct atgagacgta tttttacagt
    28081 gctcaggctg aaaaccaaat ggcaattttg aacttaatcg gtaagaaatg gatagaccat
    28141 ctgattcagc cgacgcagct tggctacggc aacggcgaca atatgcccga tgagaagctg
    28201 ctgccgctgt tcgacaaaat caatttgcag cagggcaggc attttatcgt gttgcaccaa
    28261 cgcggttcgc acgccccata cggcgcattg ttgcagcctc aagataaagt attcggcgaa
    28321 gccgatattg tggataagta cgacaacacc atccacaaaa ccgaccaaat gattcaaacc
    28381 gtattcgagc agctgcaaaa gcagcctgac ggcaactggc tgtttgccta tacctccgat
    28441 catggccagt atgttcgcca agatatctac aatcaaggca cggtgcagcc cgacagctat
    28501 ctcgtgccgc tggtgttgta cagcccggat aaggccgtgc aacaggctgc caaccaggct
    28561 tttgcgcctt gcgagattgc cttccatcag cagctttcaa cgttcctgat tcacacgttg
    28621 ggctacgata tgccggtttc aggttgtcgc gaaggctcgg taacgggcaa cctgattacg
    28681 ggtgatgcag gcagcttgaa cattcgcgac ggcaaggcgg aatatgttta tccgcaatga
    28741 gtggcgtaaa aaccaataaa gacaaattta gatgatgtcg gggaagatgc ccgaccgaca
    28801 agactatgca aaatatgaaa aaccaagtac gcggatcagg catggatgca cgatccaatc
    28861 cggccaatgt ttcagacggc ctgcaaaaca gttcgggtca tatcggtacc aacacgcgtt
    28921 accgcctgac caaactcggc gaacaaatgg cgcgcctgcc catcgacccg aaaatcgcgc
    28981 gcattttgtt ggcggcgaag aagcacgact gcatggcgga aatattggtg atcgcgtccg
    29041 cgctgtcgat tcaagacccg cgcgagcggc cgctggaagc gcgcgatgcc gccgccaagg
    29101 cgcacgagcg ttttaccgac aagcaatctg actttcttgc ctatctgaac atttgggaca
    29161 gcttccagcg cgagcgcgac aagggcttgt ccaacaagca gttggtgcag tggtgccgtc
    29221 aatatttcct gtcgcacctg cggatgcgcg agtggcgcga gctgcaccac cagcttgccc
    29281 aaaccgcgat tgaaatgggt ttgaccacca aggaagcggc tttcagacga ctttccgaaa
    29341 tcaagcagct tacgtcgtct gaaaaccaag gcgatcaaga cttatccgcc aaacgcaaac
    29401 aaaaacaact ggataagaaa caacaccgcg cccaaatccg cgccgccaaa gaagcgggct
    29461 acgaacaaat ccaccgcgcc ctgctcaccg gccttatcgc caacgtcggc atgaaatcgc
    29521 ccgacggcaa cgactatacc ggcgcgcgcg gcagccgttt ccaccttttc cccgcctccg
    29581 ccctgttcaa agccaaaccc aaatgggtga tggcggcaga actcgttgaa acgacaaaac
    29641 tttatgcgcg cgacgtcgcc gttatccagc ccgaatggat tgagcaggaa gccccgcacc
    29701 tcgtccgcta ccactatttc gagccgcact gggaacaaaa acgcggcgaa gtcgtcgcca
    29761 gcgaacgcgt gacgctttac ggcctgaccg tattgccgcg ccgccccgtg ccttacggca
    29821 aagttgcgcc cgaagaagca cgtgaaatct ttatccgcag cgcgttggtg gcgcaggaat
    29881 gcgatttgaa agcggatttt tttgtccaca ataaaaagct gattaaagaa attaccgaac
    29941 tcgaacacaa atcgcgcaag caagacgtat tggtcgacga cgaagccctg tttgcgtttt
    30001 ataacgaacg cctgcccgaa atggcttgga aagacgcgca aggcagcgtt tggggaagtg
    30061 aagattccgt acggattatt gaatctgaca aagccgagag gtcgtctgaa aatgagcgca
    30121 acgagtttcg taaaaacaag cgtaatgggt ctcgccaaaa tgaaaatcac ggcaacaccg
    30181 taggttgggt tgaaaaccca acatcagccg caactgcaaa aactgttggg tttgacaacc
    30241 caacctacgc tactcagcaa cccaccccct cccccgcgag ggagggtcgg ggagagggca
    30301 aaacagttgc cgcacaaacc aacttttccg caaccgcagc aaaccctctc ccgcaggaga
    30361 gggagcagag tgccgcagct tcagcggttt caaacgacct gcatcctgca aatccccaac
    30421 aaaccgcccc ctcccccgtg ggggagggct ggggagaggg caaaacagtt gccacccaaa
    30481 ccaacttctc cgcaaccaca gcaaaccctc tccctaaccc tctcccacag gagggggaac
    30541 agagtgccgc agcttcagcg gtttcaaacg accctcaacc ccaaaagcag ccagcacccc
    30601 aaaaagaccg tctgaaaccc ctccccctcg ccgacatccg caccttccaa gcctggctca
    30661 aaaccgccga acgcgagaac ccgcgcctgc tgttcctcag ccgcgacgat ttgatgcaac
    30721 acgccgccgc acacattacc gaagaacaat tccccaaatt ctggcaaacc gcagacggca
    30781 agttcgaact cagctaccgc ttcgagccgc accacccgct cgacggcgtt accctcaccc
    30841 tgccgcttac cgtgctcaac cgcatcagcc ccgccgccct cgaatggctc gtgcccggta
    30901 tgttgcgcga gaaaatccag ctgctgatta aagcactgcc caagcaaatc cgccgcatct
    30961 gcgtgcccgt gcccgaattc atcacccaat ttttaagcca aaaccccgac cgcaacgccc
    31021 ccatcctgcc ccaactcgcc caagccatcg ccaaaaccgc aggcgacatc cgcatattcg
    31081 agcaaatcaa ccaagacgaa tgggccgcgt tcaggctgcc cgaacactgc tatttcaacc
    31141 tccgcatcat cgacgacggc ggacaagaat tagccatagg gcgcgattta atccaaatcc
    31201 aacaacaact cggccaagcc gccgccgtta ccttccgcga caacacccaa gaattcgagc
    31261 gcgacaacgt gaccacatgg gacatcggca tcctgcccga atccatcaaa ttcgcacgcg
    31321 gcaaacaaca gcttaccggc tatctcggcc tgcaaaaaga aaaagacggc cgcatcgccc
    31381 tgcgcctgtt cgacacaata gaagccgccg aacaggcaca ccgtcaaggc gtgatagaac
    31441 tcatgaaact acaactaaaa gaacaagtta aagacctgaa caaaggcatc caaggcttca
    31501 cccaagccgc catgctgctc aaacacatca acgccgacac cctgcgcgac gacctcaccc
    31561 aagccgtctg cgaccgcgcc tttatcggcg aagacgagct gccgcgtaac gaaaaagcct
    31621 ttaaagagca aatcaaacgc gcccgcagcc gcctgcctgc cgtcaaagaa gccctcagcc
    31681 gctacctgca ggaaaccgca gccgcctacg ccgaactcaa cagcaaactc ggcaaacacc
    31741 cattgaccca cctcctacgc caacgcctgc aaaccctgct cgccgccggc ttcgccaccc
    31801 gcaccccgtg ggcacaatgg ccgcgcctcc ccatctacct caaagccatg accctgcgcc
    31861 tcgaaaaata cagcagcaac cccgcccgcg acgcagcccg cgaagccgat atccaagagc
    31921 tggaacaaat gtggcaggaa aaaacagaca gcctgattaa acaaggtctc cccatttcag
    31981 acggcctcgc cgcgtttaaa tggatgattg aagaattgag ggtgtcgctg ttcgcgcagg
    32041 aattgaagac accgtatccg gtgtcggtga agcggttgtt gaagatgtgg gaggatctta
    32101 attaaaggaa gatgccgtct gattttttca gacggcatct tttatttccc tgaccaaccg
    32161 atttaatcgc tccctaccaa tagctttatt ttttaattcg cactcccaaa cgacaatcac
    32221 tttgaaaccg atcttttcca gctcggtttc atttttaata tcacgttcgc aattttttgt
    32281 gattttttcc aaccaaaaat ccgtattgct tttcgggata tgtcctttat tacaagaatg
    32341 cccgtaccaa aaacagccgt gtataaacac gactgtttta tatttcggca aaacgatatc
    32401 cggtttgcct gcatagcgtt tatcgttttt ccgataacga aaaccttggg agaaaagaaa
    32461 tttccttacc aatatttccg gtttggtttc tttgctatgg atttttgaca tcacgaaaga
    32521 acgtttggat gtagtgaaaa tatcggtcat aaattacttg gaagatacca ccttcgatcg
    32581 tttttttgtc tgttccactt ttcttaacaa atacgaaacc aagcttatta aatccaaagc
    32641 atccttatca ttaaaaatac taggagataa atttgctctt aactcatgtt gtacaggatt
    32701 tctaaatcca gtccatgttc cgcatgcaag ctgttcaaga ccatcttcta aattttgttc
    32761 cgccttcgtt ttgttattcg ttaatagaag tatagaattt ttactcccga aagcttgttc
    32821 aatcaattct cttccgtcta tgctatgaag acctgtttgc ttctgaacct tattttcgaa
    32881 aactttaact gcctcgattg cagcacttaa ataatccttc ttttcattgt aaaaatcatt
    32941 acaggcagta tgcaaatcct gatgcaaatg ccgccaatga tagtaaggat attctggaat
    33001 aatactatgt aaggcagaga ttacttccgc ttgctcttta tttgtaagct ccgaatcttc
    33061 caaacgattc aaaataactt ctactggttc cctttccttt tctggcaatt tgtttttcca
    33121 attttcgata tcaaccccgc tttcaccttt cacttcgtca tctttttctt tttttcttct
    33181 ttttctccaa tcttgcccga tggctcgaac gatttcccca aggaaaaccc gtaactttga
    33241 agttttagga tgcttccaat ccaatgatgt cctatctgta gcaattaaat cttcatcatc
    33301 attatctata aaatcaatac ttaaccatcc tgtcagatag gaatagaaat ggctggattc
    33361 gctatcagtg aaaaactcgg gcatatttac cattctgccg ttggcaaaca aggtaatacc
    33421 tttcatatta ttctttaaag gtttttccgt tgtaatgaac ttaccttgta ttaatccgct
    33481 gtattcatac tgttcgaatt ttgaagataa cgatgaaata ttggttgcta gatcctggta
    33541 ttcccattcg aattgtgggg taacaagatc atattttaga tttccatcaa tgattttggg
    33601 ttccgagccg ttcaagctta ctttgatttt aaaatcctga gccggaaaat caaacaagcg
    33661 ggaaagatgc tccacataat tatctaacgg gtatccttgt tttttcgtca gttcggttaa
    33721 agttatggtt gttccgtttt cggtattggg ttcaacagat tctttctgaa actccggttg
    33781 ataaatacgc tcactttttt taatctctgc ataatccaaa gtaaaagtaa cccgttcgtt
    33841 tccttggata gtagaaattt caattttgtt gccaagcccg aataatgcca atttaccaag
    33901 gcctttttta cccgttggaa ttcttccgca cggggaggct tgtttttctt cccttctgtt
    33961 ccgaccgatt ctcaaataaa aatcattgat ttcatcgaag ctcattccta taccattatc
    34021 tttaataacg attttatgct ccggtttatc gaataaccta acttccactt ccgtagcaca
    34081 tgcatcgtag gcattcgcta tcaactcggc aatcgcagga acagtgtgcg aatacatctt
    34141 tacacccaaa tgttcgatta cggttggctc gaaactcatt ttcaattttt cttcagacat
    34201 gttttctcct tttctaacat tcgatattat caacaattgc cctgcctatt ttttccgcat
    34261 acatcggagg aacggcatta ccaatcaaac gggctatctt gtccctgctg cccgctttaa
    34321 agacatagtt acgagggaac gattgcaatg ttgcaccttc gcgcagtgat aacgccctat
    34381 cttcttcagg atgggcaaac ctcccgttgg aaatactaaa gaatttggtc gttatggtag
    34441 gtgcgggctt atcccaccat agcctaccaa acgtgtcttt aaaactattg tcttttccga
    34501 taaaacattc cagctgtaat tcgggaatat gcgcaaaagc caaacggttt cctccgtttt
    34561 taggtatcaa agccaatcgt ttcaagttga tatcagataa tcccgcacag ctatgcataa
    34621 aatccgtttc gtcttggtgt cctgcaataa ttttgggaaa gccattttcc attcccaaaa
    34681 catcgcgtac cgtaagccgt ttgcccgaat acttgactgg ttccagcttt tctttggtta
    34741 ttctgtttgc aattaacgta aatcttttac ggctttgggg aattccgtaa tcagccgtat
    34801 tgtgaatacc gaaatggacg gtaaatcctt tttcttccaa taactttatg aaattatcaa
    34861 gcctgctttc tttcattctg ctcagaattc ccggtacgtt ctctacaaca acatatccgg
    34921 gattgaaata ttctacaaac cgctgaaact ccaaaagcaa actcttggat ttttccgatt
    34981 ttcttttgtc tgtttgaata acactccaat attgacaagg actacaacct ataagaatca
    35041 aatcatcatc attctttttt aacccgagtt ctttttccaa ggtttcaggc tgcaattcaa
    35101 aaacatcagc ctgtataaat ttggcattta tattggcttc atatgtttct ttgcagctgg
    35161 gttcgtaatc tatcccggct aaaacctgaa taccggcgga ttgcattcca tagctcattc
    35221 cacctccgcc acagaaaaaa tcaaccgcct ttaacttact gtttttcatt tctatttaaa
    35281 taattagact agtgagaact aactttatat aacatataat ttattgctta tcaaaaataa
    35341 tttgagattt tattaaaatt catgcatcta aataagcaga acttaatttc ctccaaacga
    35401 gcggatgtct tcaaatgcac acttcacaga aaggtcgtct gaaacccatt ttcagacgac
    35461 ctttttccac ttcccaaaac aagaattccc accccctccc caaacgtcgc ccgaaccagt
    35521 aaactcccca tatttcaaca acttcttgga agccaaccat gccccgtatc caccctcctc
    35581 acctgttccc gccccatatc gccgaacgcg gccttttgta ttttcagcag ggcaaggttc
    35641 gcgatgtccg aaaaacttcc gccgggcgtt atcgggcctg gcgctgtttg ctgccggcgg
    35701 tctgaataat ggtggcgtca acgacggcag cggatgcttt ctctattttt aagccttttt
    35761 cggtcagttg gcggttaatc agtttgagca attcggacag gggcggattc gcatttgaag
    35821 tgcaactttc cctaaca
//
LOCUS       DAWEWP010000002        33738 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWEWP010000002 DAWEWP010000000
VERSION     DAWEWP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.002"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..428)
                     /locus_tag="WGN53_000028"
     CDS             complement(<1..428)
                     /locus_tag="WGN53_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139080.1"
                     /translation="MDQAKRTAYVKIINALFDGAVKVQPSQITETVVDMVDKMFDEIA
                     TCHERIRPMAVLADGIAGSIVKLAPNEIPLIFGNSSFGSYLKSKTRDEVFKVLKQSKV
                     NNAGKVLEAFYESWLKYTAKDRRLKVCIVTAQSRWRSRFE"
     gene            complement(499..4764)
                     /locus_tag="WGN53_000029"
     CDS             complement(499..4764)
                     /locus_tag="WGN53_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0139081.1"
                     /translation="MGGKSGGGQRTPYEAPNSLSSAQSLRIIDAVSEGVVSGFANGDD
                     APFKSVYFDDTPVQNSDGSYNFNGVTGYFQRGEQDQSYVPGFDASERTVAVSAAVKQN
                     QPMVRAVTDELVSRLRITVAVERNAQVEDNGDTVPADTLMRVELINSNGVQASNQVRF
                     NEKSSGTYYQDVEFDVVPQVPFNIRVFRISPDSKTDKVSNNTYFSSYVEIVDAKLSYP
                     HTAFAALSIDSAQFGNQIPRRNYLMKGRLVKVPSNYNPETRQYTGGTWDGSFKTAWTN
                     NPAWVFYDVLTQPRFSTLARRLNVADIDKWSLYQIGKYCDEPVDDGFGGKEPRFVCNA
                     YITDLMQAGEFLNNLASVFTGLPVWNGQQVSVVMDADADPVAQYTNANVKDGLFNYAG
                     AALKSIHTAVHVQYIDKYDGYRAKTEYVADNEAIARYGLNIKQITAFGCDSRGQAARF
                     GAWTLQTELRQQNAVTFEIGREGLKHLPYDIVQIMDNQYAGAELSGRVAAISGNVLTL
                     DRPVSDAVGALVFYSEGGSLKSAKITAAAGNKVTLEREVPLQAGDTWVLSGKVKPRLY
                     RAIGIKENTDAGTYTVTALLHDPKKYAAVDSFAHFDHEITTLHNTAPVLTDAAVNTDG
                     GTVVVTWDNLAANGQVLTYDIKIYRNNSLYRHIPDAQTAEIRLENLPNGNYRAEIRGR
                     NARGVLSAPLVKAWSIDYTVTGLRTTPKTLSVTVDWVLPQTVVSDLVFELWYSKTNNL
                     QTATKLASLAYPQNSYTLTGVGVADVYYFWVRIRDIAGNTGEFTAAVQGRADNNPAPI
                     VQQIQGAIGKSALSRELIDTLNRDMAAAAGLKVSAEAAERAKALQEEARARTAAIQAE
                     SSARTAELARKSSEIGTRIAATEAVNEQQAQQIQTVTSAQGNTAAGLEAEKKARTDGD
                     RAEAQARETLAVRLGNAESGISALRETVTRQDAARSSEINTLTAKLENVEVGGRNYAL
                     STGTAGKVLTASENNQTKTVTIDVSSALDLKQGDSLIISCDIELVNATSPHGKPYPRI
                     GAEFSVIYADNSVGYFAAWYGEAVSGTTKTLKQRIVAKHTVAKEVKALRSFIVQARYQ
                     TSESIKVSNVKLERGTVATDWSPAPEDSNADTSALTTLSAKVQQASSAVAEIGGKIQS
                     LYTLKTEAIAGGRRAVAGVAVGADGKTGSGEILLMADKVAYVDPKDKTVTPAFVTVIE
                     NGKAKQALNGDLFATGSILAKHIAANQTIEAPTLNGATVNAGLIKGGTIRGARIEAVD
                     LEAANIIGDVVAVRTFELKQSPLGYQELICQIPDSRKTRRTVIVPPVFAVAENGAVVS
                     LQLHMDGVIQREVTIQSPEKRIARNEKFSVWVPGPPPLYIRTPQEVPLSFSVPFHLPK
                     TGSISFQKGIDGAAHSLHILCRISGNFDYSPLGDLSGIVCLIV"
     gene            complement(4900..5622)
                     /locus_tag="WGN53_000030"
     CDS             complement(4900..5622)
                     /locus_tag="WGN53_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HEZ0139082.1"
                     /translation="MITVCLYGGLREYGRRFVLHVETPAEALHALFTQIKGLRQRIRD
                     GVYQVRFDGKDQSEETIGSVFRRPADGVLHIVPRVQGAGKNGGLIQTIVGVVLIVVGA
                     YTSWASGAQLIAVGIGMVAGGVAQMLTKQPKLDADGKGVEESKNSAFSNLANTTAQGR
                     PVPLAYGLCYCGSRVVSQGVQSRRIEPSSTAASSGAVLNLGLSKTFVQGVAATAPNGQ
                     KYRTDFSDDSVRARNYKAAFSG"
     gene            complement(5619..6374)
                     /locus_tag="WGN53_000031"
     CDS             complement(5619..6374)
                     /locus_tag="WGN53_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0139083.1"
                     /translation="MMDLDLLSEEARREMLACAEEAVPSEMCGVLVFSYEGYEFLPLS
                     NCAENPHETFEISADDWMAAERVGEIVAVVHSHPRGEPFLSGADRWMQVETGLPWILV
                     TQGRLKLFRPVPHLRGRVFEYGKTDCGALVRDAFMLMGLVFPDHPRGDMDEDAAAGFW
                     EKHLENCGFSRVSDGLCAGDVVLTATGGHASHAVLYLGNDWMLHHAYNQLSCRVPYTR
                     YWADVTHSVWRHPDFEPAMMQALENDFTHMEQA"
     gene            complement(6371..7072)
                     /locus_tag="WGN53_000032"
     CDS             complement(6371..7072)
                     /locus_tag="WGN53_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HEZ0139084.1"
                     /translation="MSGVMLQALSAAQQDVLVDLWQVDLRPLGGRVFYFCNQVNERGT
                     AVVWQGQAYEPYPIKADGFESTSQGAGNRPTLTVSNLLGLITGAADQFGQLVGVLVVR
                     RQTYAKFLDAVNFQSGRNPTADPMQEVVGKYLVERMTALNAETATFELAAPSESDGSV
                     IPARIMLANTCCWQYRGEGCGYTGRAVADRFDMPTDDASKDVCSKTLTGCRARFGATA
                     VLPFGGFPSSDKVTT"
     gene            complement(7153..7422)
                     /locus_tag="WGN53_000033"
     CDS             complement(7153..7422)
                     /locus_tag="WGN53_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0139085.1"
                     /translation="MTETFRWRVASDNKAVHKFDVRSVRFGDGYEQRQPKSLKPKLRS
                     WEIKIVGQKALMGEIKAFFDARRGVEPFNWRPPDGVPVLVKVSEY"
     gene            complement(7444..10662)
                     /locus_tag="WGN53_000034"
     CDS             complement(7444..10662)
                     /locus_tag="WGN53_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HEZ0139086.1"
                     /translation="MNNETKIYITAETGGVVSGVEKAKQSIKSLGDVASSQGRRISDG
                     LVHSGDGAEKSSTVVSAASKRTERSLASLENAIRRDIAVKIAGGKANREYYEELARQR
                     GIDIARLNPLLSQLDRHNTQTNRATQSVKQFNNALRQTPAQITDIVTQLAGGQSPFLI
                     MIQQGGQLRDMYGGFGGMLKGLATVISPMRLAVAGLGGGIAALGYAMYQGAEESCEYR
                     KALILAGDAAGITADRMQEIAVSVGAATGGYADARAAITALVSSGKVATDNYEQFARS
                     ITLQSQATGQSIDDLVEKYTEIAKDPLKAVVSLSATYRTMTADVYQQVKALQAQGREQ
                     DAVALVQRKFSEESEDMAKRVLGNLGLIERAWKDIKESASEAWDAVKSIGRDKTKLDE
                     IAALDSFISQIETSKKHPVTQLFWGEEGERKLQEAYARRARLRAEIDKEAAAAETLRQ
                     KQKQNQNRTEGIAELEAVSERYATREQQRQRELLKNDKARKKALDGITDAKKRQEIIN
                     KHAANAKLINESFAEKKHERQKEKTDKTSYPTVSNGLRIKPGAEAGGKALGGTYAFAH
                     AMQQMLGDRLFRFGAFRDRYHIGKNSLHNSGLAFDATPSPGMGMKQKQEVVAQMTRYL
                     ESLGFKNGQDFNLKLELAGQRNKNGTVATGDHWHFNWRSREAAARFAGGVEGQVKNMA
                     HLGLFKGVEKEPLSGYEKWHEDFNRRAAEAGAKRVLEILNGNRAIGEQLKLLSDPTFG
                     DWTLKQQADARELAEKADAQDSLTAASKKYSDIVKQMTDDSKEKLDDRLFEISLIGKT
                     REEIEKLTLARLWDRQIAKAREEGAPLESIDLLERGKAEGMSNLSQMQKARADSDNDW
                     RGGIESGLKSYIDSFGTMRQAMENATVQTFDKMGDALADFVATGKLDFRSLTVSILQD
                     LSKMLIKMAIVNAMKSALGYADGGIVGGGSTQFDALFSGGGYTGYGGKYEPAGIVHKG
                     EVVFSQRDVRNHGGVAAVERLRLNGYAGGGAVGLPSVLTGVRSAGAGSMQVNITINRD
                     GSTESDSSADTEMAKHLAAAIPGMVEQWYVKNVYRENGTYHK"
     gene            complement(10655..10927)
                     /locus_tag="WGN53_000035"
     CDS             complement(10655..10927)
                     /locus_tag="WGN53_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1799 domain-containing protein"
                     /protein_id="HEZ0139087.1"
                     /translation="MNVFGFSADDFSEKETTFGVWPCNWQAVQLFIGVSTQWRIGMSG
                     ATGLDYSAVAAVMECGNIKSKKRKALLEKVRLMELEVLSMWAGEHE"
     gene            complement(10975..11286)
                     /locus_tag="WGN53_000036"
     CDS             complement(10975..11286)
                     /locus_tag="WGN53_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HEZ0139088.1"
                     /translation="MAKLTLKPDATFRHTVKIPVPGAEPADVEFEFKARGRKAMKEFT
                     EKHKDGWTADTVLDCVQGWDLEEAFDRQNVEILLDSYPMAVFAIVNGYVEEVFNAREG
                     N"
     gene            complement(11350..11991)
                     /locus_tag="WGN53_000037"
     CDS             complement(11350..11991)
                     /locus_tag="WGN53_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0139089.1"
                     /translation="MAVKLPNGATVHIATALAAEKKATVATNAAECVLTVAGHGFANG
                     DLVLFKSGWGKLNERVFQIGDVKADTFKLTGIDTSNADEFPAGSGIGAVQKITDWVQV
                     SQIVEFSTSGGEQQYVDFGFLEDDFDQQIPSTKSAMSMSIKIADDTSLSGYKAAAKCS
                     DKGGKWPLKVVLKGGGLICYNGYPSMNKTPELVRNQVMAVTLSYAISGEVNRY"
     gene            complement(12023..12442)
                     /locus_tag="WGN53_000038"
     CDS             complement(12023..12442)
                     /locus_tag="WGN53_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139090.1"
                     /translation="MTSRLTEIRNAATDVLKTKFQRVYSGRGFAPAQSQLPCVVVYVD
                     RRRTEQETFDFPPTYRHTVRLVTLVCVQAASGADELAEEMLSLIGQLFAEHSDLGVGD
                     LENLVPDLLNIDSDESGEAVTVYYQQGWQAIYFDTAV"
     gene            complement(12423..12725)
                     /locus_tag="WGN53_000039"
     CDS             complement(12423..12725)
                     /locus_tag="WGN53_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139091.1"
                     /translation="MFNEPLNVFTNPADFGETVMIGGKEVNAIFDREFITDSGFGVAV
                     ANADPQIIVTEDDLPEDVKSVVVTVRGKRYAVAETDFDGCGMVVVQLRAIHDKPTY"
     gene            complement(12718..12945)
                     /locus_tag="WGN53_000040"
     CDS             complement(12718..12945)
                     /locus_tag="WGN53_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139092.1"
                     /translation="MAKIKIIPTRSFFDGEQTFATGKKYSVDESAAAVYIREGWAAEA
                     PAKEAESPVADGGEAEDSGNASGEGGEDGSV"
     gene            complement(13003..14895)
                     /locus_tag="WGN53_000041"
     CDS             complement(13003..14895)
                     /locus_tag="WGN53_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEZ0139093.1"
                     /translation="MKKMKPDKAQMQQMSRFAVFQRESVDVEKRTVEVAFSSEESVRR
                     WFGDEVLSHAQGAVDLSRLNDGGAVLFNHDWDDQIGIIERAWIDADKRGRALVRFGNG
                     TKATEKFQDVQDGILRHISVGYRVEDMVLDNPDTDDEGYRYIVTRWMPYEISFVTVPA
                     DPTVGVGRSVEPFIENPVNPTPEKGNRNMDKNQTPTTVETSAAAIPAAAATDTNNTAE
                     RGMQNERARVSELLAIGRSYAAHGGIEAAEKVIKEGGSEAQLRAAIMANMQTKPTVTA
                     GEIGMTDKEQREFSLLRAMSAAATGKWDKAGLEREVSEELEKRHGRAAAGFFVPTDLI
                     ARAYSKGNAANGGNVIENDFREDLFIELLRNRLAVAQLGATVLDGLVGDITIPKHLTG
                     NTVQWVDENGSATESNATFGQMSLKPKTVTANTELSRKFILQSSLSAEQFARSELLKA
                     MMLGIDLAAINGKGTGNEPTGILNTAGIGAVEIGANGGAPEWKHIVALESAIAAANAD
                     IGDLAYITNARVRGLLKTKLKADGVSGYIWQDGATPLNGYRCAVSNQIPSNLTKGTAA
                     NKCSPLIFGNWSDLMIAHWGVLDVIVDPYTKSTTGAVRITTLQDVDIAVRHVESFAAI
                     KDIAAA"
     gene            complement(14876..16438)
                     /locus_tag="WGN53_000042"
     CDS             complement(14876..16438)
                     /locus_tag="WGN53_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEZ0139094.1"
                     /translation="MAKQSNAAPKKRGFFARLFGRREAVPKTARRNFAGARPVGSLAS
                     WQPQNWSADALARSDLDRLRTRARSLARDNDYMRKFLNMVESNVIGRDGFALQMRVLL
                     DNADKPDALANKAIEAAFSRWARRGVCDVTGQMSFTDLQRLLIRSVARDGEVLIRHIS
                     GFDNDYGYALQVLDIDRLDTGYNVPQQNGRNAVRMGVELNSYSRPVAYWLRTAHPGES
                     YGQTNTGNLRERVPAEQISHIFLHDRPEQRRGFPWVASAIIGLQNLSGYQEAAIIAAR
                     VGASKMGFFKQTEDADNFMPPIDGQEVDNGRGSIDLIDSVEPGTFHELPQGYDFTPFD
                     PDYPHANYDAFVKASLRGIASGLNVAYHSLANDLEGVNFSSIRSGTLEERDTWMTLQN
                     WFADAFLYDVFDRWIEAALLMGAIKMPSGKSLPAGKLDKFKACNWQGRRWSWVDPLKD
                     INAHKEAVALAVKSRRDICAEMGLDFEDVITQIEQENQMLAGKGIIADVKPAASAAEP
                     ESEDNPNEENEA"
     gene            complement(16447..16701)
                     /locus_tag="WGN53_000043"
     CDS             complement(16447..16701)
                     /locus_tag="WGN53_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139095.1"
                     /translation="MSGHDYAVELEEAREILNSLRKAYKEHAAGRGMTKRYKIKDREM
                     EFSSLADLLKQIRFWQQEIKRLEAAAGVSPRRSGRIITRF"
     gene            complement(16688..17074)
                     /locus_tag="WGN53_000044"
     CDS             complement(16688..17074)
                     /locus_tag="WGN53_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEZ0139096.1"
                     /translation="MEKQDNITPPHYRSRAVECIEFTERLNFCMGNAFKYVWRHREKN
                     GAEDLKKARWYLQRQLDSCAVMHLLEPEEYAELMDGLEKCELDDLMQHVLEEILYHAF
                     FESEDSLLAAMCGVSELLKGYGDERT"
     gene            complement(17077..17286)
                     /locus_tag="WGN53_000045"
     CDS             complement(17077..17286)
                     /locus_tag="WGN53_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139097.1"
                     /translation="MYDVFKTRAAELSGAFCSLDFWLCHKFVPLCHALCHFLTRRDID
                     TLIEEGLVEERFEQGVSKFYAKEAV"
     gene            complement(17303..20149)
                     /locus_tag="WGN53_000046"
     CDS             complement(17303..20149)
                     /locus_tag="WGN53_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0139098.1"
                     /translation="MNKKYATKAEINAAAHDRWREVLEACGIPSEILDKKHHPCPSCG
                     GSDRFRFTDGSGSSRGSGVWICNQCKPEGGSPFDLLMDVCGYSFNEARDKVASLLGLA
                     HGDLVDKAPKLLPPPAPVKEEERDLWKPIVPVPEYALNSMSFGNGFRKSDDLIFKSVF
                     RDSSSAVLGGVARFRKSDGGKIDLPYTFCEHKKTGEKMWRWRCWDNPRPLYGLDSLVA
                     DPSLPVLVVEGEKCKNAADAQGYNYAVLTWHGGCNNWDKSDWSAVVDRDVVLWPDCDS
                     LRQKLTKKDRDDGVDPESKPFLPRNEQGGMKAMLGIAEVLTKQNCRVWLVNIPAPGIW
                     PHGFDIADAIADGGRICDPSEVLSWMGAADWLVEYVPEAEMPSENSPAPIQDMEPESE
                     QLHESLEATGNQGGAGDVDDEYLAKLQKLKSEFGLVEGKDHGVNRKTGVTYTRKAMTV
                     HFGKDVVDAWYEWGRAPVLTMYEINRLKKDYALIQAVKDDDTQDMMERFIYLDGSASI
                     WDNKLWRVVPEKAARLSMTPDGFKIWVNSPSRIVKRFDRLVFEPGRVLSDDYINIFRG
                     MPIEPKFPIPKEDMPETNYELYRLFPEIKPILNLINHLCNGDYQQVEYILNWLAYPLQ
                     NVGAKMTTAVVMHGHIHGAGKSFFFEEIVKPMYGEYGATYGQEDLESNYTANRSGKMF
                     GIFEEVYTNQQKYNKTGSQKHMITGKTMRVERKFQDAYEEANHMNCVFLRNESHPFKI
                     EENDRRYFVVWPEKKLDKKLRDEVLDCIGNDGVRLFYSFLLCWNFYLTYGQQGAENEK
                     GYEVIKLEEPVRFDPNTPPPMTAAKQNVISYGRYAWQTFYSEWAGGGIKDLPFCCCIT
                     DDLWLVYKEWCRQNGERETGKTKFLQHIAEKMPRARRWWRTKNKDGDVSCQNWIFKPA
                     GWQAADGLPEMDALGNEVLRFRSAGWRYRGGEEV"
     gene            complement(20264..20611)
                     /locus_tag="WGN53_000047"
     CDS             complement(20264..20611)
                     /locus_tag="WGN53_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139099.1"
                     /translation="MNRTKTCKECGEIKPITAFPKNGFDRKTGLQKYAPRCRRCHNVY
                     QQDLLYKRREKEFEQKQAVMAALMDAEREKEEAAKARSIAQSLVLAKAACPILHIGLW
                     TEPLFIPQGMLQI"
     gene            21011..21727
                     /locus_tag="WGN53_000048"
     CDS             21011..21727
                     /locus_tag="WGN53_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0139100.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            21830..22255
                     /locus_tag="WGN53_000049"
     CDS             21830..22255
                     /locus_tag="WGN53_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139101.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYGPTD"
     gene            22486..22686
                     /locus_tag="WGN53_000050"
     CDS             22486..22686
                     /locus_tag="WGN53_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139102.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            22754..23113
                     /locus_tag="WGN53_000051"
     CDS             22754..23113
                     /locus_tag="WGN53_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139103.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVPNTSSTLKMVRSN"
     gene            23138..23992
                     /locus_tag="WGN53_000052"
     CDS             23138..23992
                     /locus_tag="WGN53_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HEZ0139104.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTLLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGIIA"
     gene            24064..24279
                     /locus_tag="WGN53_000053"
     CDS             24064..24279
                     /locus_tag="WGN53_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139105.1"
                     /translation="MTITEEYSIDIRVTAEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRKALICLAKDNLKNAA"
     gene            24315..24575
                     /locus_tag="WGN53_000054"
     CDS             24315..24575
                     /locus_tag="WGN53_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139106.1"
                     /translation="MKYLIRTALLAVVAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            24572..24865
                     /locus_tag="WGN53_000055"
     CDS             24572..24865
                     /locus_tag="WGN53_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139107.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            24868..25059
                     /locus_tag="WGN53_000056"
     CDS             24868..25059
                     /locus_tag="WGN53_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139108.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            25059..25199
                     /locus_tag="WGN53_000057"
     CDS             25059..25199
                     /locus_tag="WGN53_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ0139109.1"
                     /translation="MDTLLKIIIALSFSGAEALAVWLFITAADAVLRRFPKPSHYRRV
                     KK"
     gene            25196..25405
                     /locus_tag="WGN53_000058"
     CDS             25196..25405
                     /locus_tag="WGN53_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ0139110.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(25463..26380)
                     /locus_tag="WGN53_000059"
     CDS             complement(25463..26380)
                     /locus_tag="WGN53_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEZ0139111.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMSRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(27248..27727)
                     /locus_tag="WGN53_000060"
     CDS             complement(27248..27727)
                     /locus_tag="WGN53_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ0139112.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(28163..28543)
                     /locus_tag="WGN53_000061"
     CDS             complement(28163..28543)
                     /locus_tag="WGN53_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0139113.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(28698..29894)
                     /locus_tag="WGN53_000062"
     CDS             complement(28698..29894)
                     /locus_tag="WGN53_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEZ0139114.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWVYPTGGRSWK
                     LSFVQDGRQQTISLGRYPDFSLADAREWREEVRRKRAHGENVVNKKVRADFAFEKVAR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERAKTGNMAALSPSELPRLIDFLE
                     QRNEFAVYAGRVRIHPVTRLCIYWLLLTMTRIQEAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMQWVLDQARALNVNGRFVFESVNFQGHINKESPRVAMQRAGLDTTAHG
                     LRLLARTYLREVLKVDNDVAEKLLAHSLGTRTQTAYNRSELWEERKDALERWGNDVLR
                     LAKNGK"
     gene            complement(30086..30175)
                     /locus_tag="WGN53_000063"
     tRNA            complement(30086..30175)
                     /locus_tag="WGN53_000063"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30139..30141),aa:Ser,seq:cga)
     gene            complement(30253..30343)
                     /locus_tag="WGN53_000064"
     tRNA            complement(30253..30343)
                     /locus_tag="WGN53_000064"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30307..30309),aa:Ser,seq:tga)
     gene            complement(30426..31205)
                     /gene="yaaA"
                     /locus_tag="WGN53_000065"
     CDS             complement(30426..31205)
                     /gene="yaaA"
                     /locus_tag="WGN53_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ0139115.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLNTDQIQ
                     YLQGRVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(31467..32654)
                     /gene="dapC"
                     /locus_tag="WGN53_000066"
     CDS             complement(31467..32654)
                     /gene="dapC"
                     /locus_tag="WGN53_000066"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ0139116.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFEWVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            complement(32730..33182)
                     /gene="dut"
                     /locus_tag="WGN53_000067"
     CDS             complement(32730..33182)
                     /gene="dut"
                     /locus_tag="WGN53_000067"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ0139117.1"
                     /translation="MNIEVEMKVLDERMADVVPVYATKGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVVVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            33325..>33738
                     /locus_tag="WGN53_000068"
     CDS             33325..>33738
                     /locus_tag="WGN53_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ0139118.1"
                     /translation="MTHISSPRNEFIRGIKESSPMLIGLLPWALILGMQGGQKGMSWL
                     EMLLMTGMNFAGGSEFATVNLWAEPLPILLIATVTFMINSRHILMGAALAPHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
BASE COUNT         8312 a         9446 c         7703 g         8277 t
ORIGIN      
        1 atttcaaagc gcgaacgcca acgggattgc gcggttacga tgcagacttt cagacggcgg
       61 tcttttgcag tgtactttaa ccagctttcg taaaatgcct caaggacttt accggcatta
      121 ttaaccttgc tttgcttcaa cactttaaac acttcatcac gggttttact tttcagatag
      181 cttccaaacg acgaattccc aaaaataagc ggaatttcgt ttggcgcaag tttgacaata
      241 cttcctgcaa taccgtctgc caacaccgcc atcggcctga tgcgctcatg gcaggtcgca
      301 atttcgtcaa acatcttgtc caccatatca acgacggttt cggtaatttg actgggctgc
      361 accttgacag ctccgtcgaa taaagcgtta ataattttaa cataggctgt acgttttgcc
      421 tgatccataa ataaacctcc ataaaaaagc ccaccctcag gcaggcagaa aaaagccgtc
      481 ctttcggacg gctaaaattt aaacaataag acagacaata cccgataaat cgcccaaggg
      541 tgaataatca aaattgccgc taatgcggca gaggatatgc aacgaatgtg ctgcaccatc
      601 gatacccttc tgaaaagata tactgcctgt ctttggtaga tggaatggga cggaaaaact
      661 aagaggcacc tcctgcggag tccttatata cagcggcgga gggccaggaa cccatacgga
      721 aaacttttcg ttacgcgcta tcctcttttc aggtgactga atcgtaacct cccgctgaat
      781 cacaccatcc atgtgcagtt gtagactgac aactgcacca ttttctgcaa cagcaaaaac
      841 cggcggcacg ataaccgtcc ggcgtgtttt tcggctatca gggatttggc atatcaattc
      901 ctgataacct aatggcgact gcttcagctc aaacgtcctg accgccacga catctccgat
      961 gatattagcg gcctccaaat ccacggcttc aattcgcgcg ccacgtatcg taccaccctt
     1021 aattagacct gcattgacgg ttgcaccatt cagcgttggg gcttcgatag tctggttggc
     1081 agcgatgtgc tttgccagta tcgaaccagt cgcgaacagg tcgccgttca aagcctgctt
     1141 cgccttgccg ttttcgataa cggtaacgaa agcgggcgta accgtcttat ctttcggatc
     1201 gacataagca actttatctg ccatcaggag gatttcgcca cttcccgtct ttccatcggc
     1261 tcccactgca acccctgcaa ctgcccgacg accgcctgct attgcctcag tcttcagcgt
     1321 gtacaaagac tgtattttcc ctcctatctc tgcaacagca gaactggcct gctgaacttt
     1381 agccgacaag gtagtcaaag cagacgtatc ggcgttactg tcctccggtg caggactcca
     1441 gtcagttgct accgtgccgc gctccagttt tacatttgaa accttgatgg attctgatgt
     1501 ctgataccgc gcctgaacga tgaagcttcg aagtgcttta acctctttgg caaccgtgtg
     1561 tttggcgaca atacgctgtt tcagcgtttt ggtcgtaccg ctgacggctt cgccgtacca
     1621 cgcagcaaaa taacctaccg agttgtccgc atagattacg gaaaattccg caccgattcg
     1681 cgggtatggt ttgccatgag gcgatgtggc gtttaccagt tcgatatcgc acgagataat
     1741 caaactatcg ccttgcttca gatccaaagc agacgaaacg tcgatagtga cggttttggt
     1801 ctgattattc tcgctagcgg tcaacacttt gccggctgtt cctgttgata aggcataatt
     1861 ccgaccgccg acttcaacat tctccagctt cgccgtcaac gtgttgatct cagacgacct
     1921 ggcagcatcc tgacgtgtta cggtttcccg caatgccgaa ataccgcttt ctgcattacc
     1981 aagtctgaca gccagtgttt cacgcgcctg cgcctcggct ctgtcgccat cggtacgggc
     2041 ttttttttcg gcttccagcc cggcggcggt attgccttgc gcagacgtaa cagtttggat
     2101 ttgctgcgcc tgctgctcat tgacagcttc tgttgcggca atacgcgtac cgatttcgga
     2161 tgactttctg gcaagctcgg cagttcgggc agatgattcc gcctgtattg ccgccgttcg
     2221 cgcccgtgct tcctcctgta aggctttagc acgttccgct gcctctgcag acactttcaa
     2281 ccccgcagcc gcagccatat cacgattcag cgtgtctatc agctccctgc ttaatgcaga
     2341 cttcccgatt gcaccctgaa tctgctgcac tatcggcgca ggattgttat ctgccctacc
     2401 ctgtacggca gcggtaaatt cccccgtatt tcctgcaata tcacgtatgc gcacccagaa
     2461 ataataaacg tcggcaacgc cgacgccggt taaggtataa ctgttttgcg gatacgccag
     2521 cgacgcgagt ttcgttgccg tctgaagatt gttggtcttg ctataccaca gctcgaaaac
     2581 caaatcggaa acaacggttt gcggcaatac ccaatccacg gtcacagaca gtgttttcgg
     2641 cgtcgtcctc aatccggtaa cggtatagtc gatactccaa gccttaacca acggtgcgga
     2701 cagtacaccc cgtgcattac ggccgcgtat ttccgcccga taattgccgt tcggcagatt
     2761 ttccaaacga atttccgctg tctgtgcatc agggatatgg cggtataggc tgttgttacg
     2821 gtaaatctta atatcgtaag ttaaaacctg accgtttgcg gccaggttgt cccatgtcac
     2881 cacaactgtc ccaccgtcgg tattgacagc cgcatcggtc aacacagggg cagtgttgtg
     2941 cagcgtggta atttcatggt caaaatgcgc aaaactatcc acggcagcat attttttcgg
     3001 gtcatgcagc aacgcggtaa cggtataagt acccgcatcc gtattctctt tgatgccgat
     3061 ggcacggtag aggcgcggct tgactttgcc ggataaaacc cacgtatcgc cagcctgcaa
     3121 cggcacctca cgctccaaag taaccttatt gcctgctgcc gcagttatct tcgctgattt
     3181 cagactaccg ccttcagaat aaaacaccaa ggctccgacc gcatcggata caggtcgatc
     3241 cagcgtcagc acattgcccg aaatggcggc aacacgtcca gacaactctg cacctgcgta
     3301 ttgattgtcc ataatctgca cgatgtcgta aggcagatgc ttcaacccct cacgcccgat
     3361 ttcaaacgtt acggcgttct gctgccgcaa ctcagtttgc agcgtccatg cgccgaaacg
     3421 tgccgcctgc ccgcgcgaat cgcagccgaa cgcagtaatc tgcttgatat tcaaaccata
     3481 gcgggcaatg gcttcgttgt cggctacata ttctgtttta gcgcgatagc catcgtattt
     3541 gtctatatac tgcacatgaa cagcagtatg aatggacttc aacgctgcac ccgcataatt
     3601 aaacaggccg tctttaacat tggcattggt atattgcgcc accggatcgg cgtcggcatc
     3661 catgacgacg gatacttgct gtccgttcca aaccggtaaa ccggtaaaga cgctggcaag
     3721 attgttcaaa aattcacctg cctgcatcag gtcggtaatg taggcgttgc agacgaaacg
     3781 cggctctttg ccgccgaagc cgtcatcgac cggctcgtcg caatatttcc cgatttggta
     3841 caggctccat ttgtctatgt ctgccacatt cagacgacgt gccagcgtgg aaaatctcgg
     3901 ctgggtcagc acatcgtaaa aaacccatgc cgggttgttt gtccatgcgg ttttgaaact
     3961 gccatcccat gttccacccg tatattggcg ggtttcagga ttgtaattgg acggcacttt
     4021 gaccaagcgg cctttcatca aataattgcg acgcggaatc tgattcccga actgcgccga
     4081 atcaatcgac aatgccgcaa acgcggtatg cggatagctc aatttcgcat caacgatttc
     4141 aacgtaggac gaaaaatagg tattgttgct gaccttgtcg gtcttggaat caggcgaaat
     4201 tcggaaaacg cggatattga acggcacttg cggcaccaca tcaaattcga catcctgata
     4261 atatgtcccg ctggattttt cattgaaacg cacctgattt gatgcctgca caccattgct
     4321 gtttatcaac tcaacgcgca ttaaggtatc agcaggcacg gtgtcgccgt tgtcttcaac
     4381 ctgtgcattc cgctcgacag cgaccgtaat ccgcaaacgg ctgaccaatt cgtcggtaac
     4441 ggcgcgaacc atcggctgat tctgcttcac cgcagccgaa acggctacgg tgcgctcgga
     4501 agcatcgaaa cccggaacat aggactggtc ttgctcgcca cgctggaaat aacccgtaac
     4561 tccattaaaa ttgtagctgc cgtccgaatt ttggacagga gtgtcatcaa aatacacgct
     4621 tttgaacggc gcatcatcgc cgttggcaaa ccccgaaacc acaccttcgg ataccgcatc
     4681 aataatccgc agcgactggg cgctggataa actgtttggg gcttcataag gtgtccgttg
     4741 tcctccgccc gatttgccgc ccatattgtt tcctttcctg attcaaaacc ttacttcacg
     4801 gtattcagaa taccgatcaa agcccaaaaa tacacaaaca aaaacagaca cgccgcgaag
     4861 tttgctcctt cgcggcgttt cgatttttca gacgacattt cagcccgaaa acgccgcctt
     4921 ataattgcgc gcccgcacgc tgtcgtctga aaagtcggta cggtatttct gcccgttcgg
     4981 cgcggttgct gctacgcctt gaacgaatgt cttggacaaa cccaaattca acaccgcacc
     5041 tgacgaagcc gccgtcgatg acggttcgat acggcgggac tgcacaccct gcgatacaac
     5101 acggctgccg cagtagcaaa gcccgtaagc caacggcaca gggcgaccct gtgccgttgt
     5161 attggcaagg ttggaaaaag cagaattctt actctcctcc acccccttgc cgtcggcatc
     5221 caatttgggc tgtttggtca acatttgggc gacaccgcca gccaccatac cgataccgac
     5281 ggcgataagc tgcgcaccac tggcccaact cgtatacgca ccgactacaa tcaaaaccac
     5341 tccgacaatg gtttggatca acccgccatt tttacctgca ccctgtacgc gcggaacaat
     5401 gtgcaataca ccgtcagcag gtcgtctgaa aacgctacct atcgtttctt ccgactggtc
     5461 tttaccgtca aaccgaacct gatacacccc gtcccgaatc cgctgacgca agcctttgat
     5521 ttgggtaaac agagcgtgca gggcttcggc tggcgtctcg acgtgcaaaa caaaacggcg
     5581 gccgtattcg cgaaggccgc cgtacagaca aacagtaatc atgcttgctc catatgggta
     5641 aaatcgttct caagtgcctg catcatggcc ggctcgaaat cgggatgccg ccaaacgctg
     5701 tgcgtaacat ccgcccaata gcgggtataa ggtacacggc agctcaattg attgtaggca
     5761 tggtgcagca tccagtcgtt gcccaaatac aggacggcat gggacgcatg cccgcccgtt
     5821 gccgtcaaga ccacatcgcc ggcgcaaagg ccgtctgaaa cacgggaaaa cccgcagttt
     5881 tccaaatgct tttcccaaaa gcccgccgct gcgtcctcat ccatatcacc gcgtggatgg
     5941 tcgggaaaaa ccagccccat cagcatgaag gcatcgcgca ccagcgcgcc gcaatccgtc
     6001 ttgccgtatt cgaatacacg cccgcgcaaa tgcggaacgg ggcggaacag tttcagacgg
     6061 ccttgtgtaa ccaaaatcca aggcaagccc gtttcgacct gcatccatct gtctgcaccc
     6121 gataaaaacg gctcgccgcg cggatgggaa tgcacgactg ccacaatctc gccgacgcgc
     6181 tccgccgcca tccagtcgtc agcggaaatc tcaaaggttt catgcggatt ttcggcgcaa
     6241 ttggacaaag gcagaaactc atagccttca taactgaaaa ccaaaacccc gcacatttca
     6301 gacggtacgg cttcttcagc acacgccagc atttcgcggc gggcttcttc acttaacaaa
     6361 tccaaatcca tcatgtcgtt accttatcac tagatgggaa accgccgaac ggcaaaactg
     6421 ccgtcgcgcc aaaccgcgcc ctacatcctg tcagtgtttt gctgcatacg tctttggacg
     6481 catcgtccgt gggcatatcg aaacggtcgg caaccgcacg ccccgtataa ccgcaccctt
     6541 cgccgcgata ctgccagcag caggtatttg ccagcataat acgggcggga atgaccgaac
     6601 catccgattc ggacggcgcg gcaagttcga aggttgccgt ttctgcattc aatgccgtca
     6661 tccgttcaac caaatatttg ccgacgactt cctgcatcgg gtctgcggtc ggattgcgcc
     6721 cggactggaa attgaccgca tctaaaaact tggcataggt ttgacggcgc acaaccagca
     6781 cgccgacgag ctgaccgaac tgatccgccg cgcccgtaat caaacccaag agattcgata
     6841 ccgtcagcgt cggacggtta cccgcgcctt gcgaagttga ttcaaagccg tcagccttaa
     6901 tcggataagg ctcatatgcc tgtccctgcc agacaaccgc cgtaccccgc tcgttgacct
     6961 gattgcagaa atagaaaacc cgaccgccca gcggccgcaa gtccacttgc cacaaatcga
     7021 ccaaaacatc ctgctgcgcg gcagacaagg cttgcagcat cacacccgac atggccttta
     7081 tacgcggatt catgaaagca cctcctcaaa cgtacagctc aattcgtagg ctttgccacc
     7141 cttcgccgtt tcctaatatt ccgatacctt gaccaataca ggcacgccgt cgggcggtct
     7201 ccaattaaac ggctcaacac cgcgccgggc atcaaaaaag gctttgattt ctcccatcaa
     7261 agccttttgt ccgactatct taatctccca gcttcgcagc ttcggtttca aagatttagg
     7321 ctgccgttgc tcgtagccgt cgccaaagcg gacggaacgg acatcgaatt tatgtacagc
     7381 cttattgtcc gaagcaacac gccatcggaa tgtttcagtc atgatcggtt cctttcagac
     7441 gacctattta tggtaagtac cgttttcacg gtacacattc ttgacatacc actgttccac
     7501 catgcccggt atggcagcgg caagatgctt cgccatctcc gtatcggcac tgctgtccga
     7561 ttcggtgcta ccgtcccgat ttatagtgat gttgacctgc atgcttccag ccccggccga
     7621 acggactccg gtcaaaacgg acggcagtcc tactgcgccg ccaccggcat aaccattcaa
     7681 ccgcagccgc tcgacagccg caacgccgcc gtgattacgt acgtcccgct gggaaaatac
     7741 gacttcgccc ttgtgaacaa tgccggcagg ctcatacttc ccgccgtagc cggtatagcc
     7801 gccgccggaa aacaacgcgt cgaactgcgt acttcctcca ccgacaatac cgccgtcggc
     7861 ataacccaac gccgacttca ttgcattgac aatcgccatt ttaatcagca tttttgacaa
     7921 atcctgcaaa atggaaacag tcaggcttcg aaaatcaagt ttgcctgtgg caacaaagtc
     7981 tgccagcgca tcccccattt tgtcgaaggt ttgtacggtg gcattttcca tcgcctgcct
     8041 catcgttccg aatgaatcga tgtagctttt caagccggac tcgataccgc cgcgccaatc
     8101 attgtcgctg tctgcacgcg ccttctgcat ttgagaaaga ttgctcatcc cttccgcctt
     8161 gccgcgctcc aataaatcga tactttccaa cggcgcacct tcttcacggg ctttcgctat
     8221 ctgtctgtcc cacagtctcg ccagcgtcag tttctcgatt tcttcacggg ttttgccgat
     8281 aagcgaaatt tcaaacagcc tgtcatccaa cttttctttc gaatcgtcgg tcatttgctt
     8341 cactatgtcg gaatattttt tggaggcggc cgtcaaacta tcctgcgcat cggccttctc
     8401 cgccagctcg cgggcatcgg cctgctgctt cagcgtccaa tcgccgaaag tcgggtcgga
     8461 caacaacttc aactgttcgc cgatggcgcg gttgccgttt aaaatttcca gcacccgctt
     8521 cgctcccgcc tccgccgccc gacggttaaa atcctcgtgc catttttcgt agccggaaag
     8581 aggttctttc tccactccct taaacaaccc gaggtgcgcc atatttttca cctggccctc
     8641 aacaccgccg gcaaatcgcg cagccgcctc tcgagaacgc cagttgaaat gccagtggtc
     8701 gccggttgct accgtaccgt ttttattgcg ctgtccggca agttccagtt taaggttaaa
     8761 atcctgcccg tttttaaacc ccaacgactc aagataacgg gtcatttggg cgacaacttc
     8821 ctgtttctgc ttcatcccca taccgggaga aggagtggca tcaaatgcca atccactgtt
     8881 atgcaggctg tttttcccaa tgtggtatcg atctcggaag gcgccgaaac ggaacagacg
     8941 gtctcccagc atctgctgca tggcatgcgc gaaggcatat gttccgccta gcgctttgcc
     9001 gcccgcttcg gctcccggtt taatcctcaa gccgttcgaa acagtggggt agcttgtttt
     9061 atccgttttt tctttctgtc gttcgtgttt tttctcggca aaactttcat taatcagctt
     9121 ggcatttgcc gcgtgcttat taatgatttc ttgccgcttt ttcgcgtcag tgatgccgtc
     9181 caacgccttt ttccgtgctt tgtcgttttt aagcagttcg cgctgccgtt gctgctctcg
     9241 agtggcataa cgttccgaaa cagcctccaa ttcggctata ccttcagttc gattttggtt
     9301 ctgcttctgt ttctgccgca aagtttcagc agcggctgcc tccttgtcga tttcggcacg
     9361 caaccgggca cgccgcgcat aggcttcctg cagcttacgc tcaccctctt caccccaaaa
     9421 caattgtgta accggatgtt tcttgctggt ttcgatttgg gaaataaacg aatctagcgc
     9481 ggcaatttca tccagcttgg tcttgtccct acctatggat ttaacagcat cccatgcctc
     9541 cgacgcactt tctttaatat ccttccacgc ccgctcaatc aacccaagat tacccagtac
     9601 gcgttttgcc atatcctccg actcttcgga gaatttccgc tgcaccaatg caaccgcgtc
     9661 ctgttcccga ccttgtgcct gcaaagcctt gacctgctgg taaacatcgg cagtcattgt
     9721 ccggtaggtg gctgataggg ataccacagc tttcagtggg tctttggcga tttcagtata
     9781 tttctcaacc aaatcgtcaa tactttgccc cgttgcctgc gactggaggg taatactccg
     9841 ggcgaactgt tcgtaattgt ccgttgcaac cttgccgctt gagaccaagg ccgtaattgc
     9901 agcacgcgca tcagcatagc cgcccgtcgc tgccccgacc gaaacagcga tttcctgcat
     9961 cctgtctgca gtaatccctg ctgcatctcc ggcaagtatc aaggctttac ggtattcgca
    10021 cgattcctct gccccctgat acatcgcata acccaatgcc gcaatgccgc cgcccaaccc
    10081 cgcgacagca aggcgcatag gggaaatgac cgtggccagt cccttaagca taccaccgaa
    10141 gccgccatac atatcacgca actgcccacc ctgttgaatc atgatcagaa acgggctttg
    10201 cccgcctgca agctgggtaa cgatgtcggt aatttgtgcc ggggtttgcc gcaatgcgtt
    10261 attgaactgc ttgacagact gcgtagcgcg attggtctgc gtattatgcc tgtccaattg
    10321 ggacagtagc ggattaagcc gtgcaatatc gataccgcgc tggcgcgcaa gctcttcgta
    10381 atactctcgg tttgccttac cgcctgcaat cttaacggct atatcgcggc ggatggcatt
    10441 ctccagagaa gccaaagacc gttccgtccg cttcgatgcc gccgacacta ccgtcgatga
    10501 tttctctgct ccgtcgccac tatgtaccaa tccgtcagaa atacgccgcc cctgggaaga
    10561 tgccacatcc cccaaagact tgatggattg ttttgccttt tctacgccgc tgacgacacc
    10621 gccagtttcg gcggtaatat aaatcttggt ttcgttattc atgttcgcct gcccacattg
    10681 acaacacttc aagctccatc aggcggactt tctccaacag tgctttccgt ttcttcgact
    10741 tgatattgcc gcattccata actgctgcga ctgcggaata atccaacccc gtcgcacccg
    10801 acattccgat acgccactgc gtcgatactc caataaacag ttgcaccgcc tgccagttgc
    10861 acggccatac gccaaaagtg gtctcctttt ctgaaaaatc gtccgccgaa aatccgaata
    10921 cattcaattc cgccgcgtca ggctgcttct cataaagcgc acgcgcggcg gcaatcagtt
    10981 tccctcgcgg gcattgaaaa cttcctcaac atagccgttg acgatggcaa acaccgccat
    11041 cggatagcta tccagcagga tttcgacatt ctgccggtcg aatgcttctt ccaaatccca
    11101 gccttgaaca caatctaaga cggtatctgc cgtccagccg tctttatgct tttcggtaaa
    11161 ttctttcatt gccttgcggc cgcgcgcctt aaattcaaat tcgacgtccg caggttctgc
    11221 accgggaaca gggattttta cggtatgtcg gaaagttgca tcaggcttca aagtgagttt
    11281 tgccatttca atactccaaa taaataaggt tgcccgagta actcggacaa ccttgatatg
    11341 ccgataaaat caataacggt ttacttcgcc ggaaatggcg taggacaacg ttaccgccat
    11401 tacttggtta cgaaccaatt cgggggtttt attcatgctg ggataaccgt tatagcaaat
    11461 cagcccacca cctttcaaaa ccactttcaa aggccacttg ccgcctttat cgctgcattt
    11521 tgcagccgcc ttgtagccgg acaacgaagt atcgtcagcg atcttaatcg acattgacat
    11581 agctgatttc gtggatggga tttgctggtc aaaatcgtcc tccaggaaac caaaatccac
    11641 atattgctgc tcgccgccac tggtcgaaaa ttctacaatt tgcgaaactt gcacccaatc
    11701 ggtaattttc tgaacagcac caataccgct gcctgccgga aactcatctg cattggaagt
    11761 atcaatgccg gtcagcttga atgtatcggc tttgacatcg ccaatctgga aaacgcgttc
    11821 attcaacttt ccccagccgc ttttaaacaa aaccaaatcg ccgttggcaa acccgtgtcc
    11881 tgctaccgtc aggacgcatt cggcagcatt agttgccacc gtagcttttt tctccgccgc
    11941 caaagcggtc gcaatgtgaa cggtcgcacc gttcggtaat ttcactgcca tactttaact
    12001 ccaaattaaa ggcaatctga aatcagactg ccgtatcaaa ataaattgcc tgccatcctt
    12061 gctgataata gacggtaacg gcctcgccgc tttcgtcgga atcgatattc agcaaatcag
    12121 gcaccagatt ttccaaatca ccaacaccca aatcggaatg ttcggcaaac aactgcccaa
    12181 tcaaagacag catctcttcc gccagttcgt ccgcaccgga agcagcctgc acgcaaacca
    12241 acgtaaccag ccgcaccgta tgccggtagg taggcggaaa atcaaacgtt tcctgttctg
    12301 ttcggcgacg gtcaacgtac accacaacac acggcaactg cgattgcgca ggcgcgaaac
    12361 cacgcccgga atacacgcgc tggaacttcg ttttcagcac atctgtcgcc gcattgcgga
    12421 tttcagtaag tcggcttgtc atgtatcgcc ctcaattgca caacaaccat tccgcagccg
    12481 tcaaaatcgg tttccgccac cgcataacgt ttgccacgca cggtaacaac caccgacttc
    12541 acgtcttccg gcaggtcgtc ttcagtaacg atgatttgcg ggtcggcatt ggcaacggca
    12601 acgccaaacc cgctatccgt tataaattca cggtcaaata tcgcgtttac ctctttaccg
    12661 ccaatcatca cggtttcacc gaaatcggca ggattggtaa acacgttcag cggctcgtta
    12721 aacactgccg tcctcgccgc cctcaccgga tgcgttaccg ctatcttcag cttcgccgcc
    12781 atcggcaaca ggggattcgg cctctttggc aggcgcttct gccgcccaac cttcgcggat
    12841 atagacagcc gccgcagatt cgtccaccga atattttttc cctgtggcaa acgtctgttc
    12901 accatcgaaa aaactacggg tcgggataat tttgattttt gccattttta atacctcaat
    12961 taataaggtc gtctgaaatt cagacgacct tgggtttcac aatcaagcag ccgcgatgtc
    13021 tttaatggcg gcaaaggatt cgacatgacg gacggcaata tccacatctt gcaacgtggt
    13081 gatgcgtacc gcgcccgtag tagacttggt gtacggatca acaatcacat ccaaaacgcc
    13141 ccagtgcgca atcatcaaat cagaccagtt accaaaaatc agcgggctgc atttgttggc
    13201 cgccgtgcct ttggtcaggt tggacggaat ttggtttgat accgcgcaac ggtagccgtt
    13261 caacggcgtt gcgccgtcct gccagatgta gccggacacg ccgtcggcct tcagcttggt
    13321 tttcagcaga ccgcggacgc gggcattggt gatgtatgcc aaatcgccga tgtcggcatt
    13381 ggcggcggca atggcacttt ccaaagcgac gatatgcttc cattcgggcg caccgccgtt
    13441 cgcaccgatt tccaccgcgc cgatgccggc ggtattcagg atgccggtcg gttcgttgcc
    13501 ggtacctttg ccgttgatgg ccgccaaatc gatacccagc atcatcgctt tcaataattc
    13561 gctgcgggcg aactgttcgg cagacagcga ggattgcaaa atgaatttgc ggctcaattc
    13621 agtattggcg gtaacggttt tcggtttcag gctcatttgt ccgaaagtgg cattcgattc
    13681 ggtcgcgctg ccgttttcat ccacccattg aacggtgttt ccggtcaggt gtttcggaat
    13741 agtgatgtcg ccgaccaagc cgtccagtac ggtggcgccc aactgggcaa cggcaagtcg
    13801 gttgcgcagc agttcgatga acaagtcttc gcggaagtcg ttttcgatga cgttgccgcc
    13861 gtttgccgca ttgcctttgc tgtaagcgcg ggcaatcaaa tcagtcggca caaagaagcc
    13921 tgccgctgcg cgaccatgtc gtttttccaa ctcttccgac acttcgcgtt ccaagcccgc
    13981 tttgtcccat ttgccggttg cggcggcaga catggcgcgg agaagggaaa attcacgctg
    14041 ttctttatca gtcatgccga tttcaccggc ggtaacggtc ggcttcgtct gcatgtttgc
    14101 catgatggcg gcgcgtaatt gtgcttcact gccgccctct ttaataacct tttcggctgc
    14161 ttcgataccg ccgtgggcgg cgtaactgcg accaatggcc agcagttcgg aaacgcgcgc
    14221 gcgttcgttc tgcataccgc gttcggcggt gttgttggta tcggttgctg cggcggcagg
    14281 gattgcagcg gcggaagttt ccaccgtagt gggcgtttga tttttatcca tgtttcgatt
    14341 tcctttttca ggggttgggt ttacagggtt ttcaataaat ggttccaccg acctgcctac
    14401 acctactgtc gggtctgcag gaacggttac aaaactgatt tcatacggca tccagcgggt
    14461 aacgatgtaa cggtagccct catcgtcagt atcgggattg tccaatacca tatcctccac
    14521 tcggtagccg acgctgatat ggcgtaggat gccgtcttgc acgtcttgga atttttccgt
    14581 tgccttcgta ccgttgccaa aacgtaccaa ggcacggccg cgcttgtcgg catcaatcca
    14641 agcacgttcg atgatgccga tttggtcgtc ccagtcatga ttgaacaaca ccgcgccgcc
    14701 gtcgttcagg cggcttaagt caacagcacc ctgcgcatga cttaatactt catcaccgaa
    14761 ccaacggcgt accgattctt cgctggaaaa cgccacttcg accgtccgtt tttcaacatc
    14821 aacactttcg cgctgaaata cggcaaagcg gctcatttgc tgcatttgcg ccttatcagg
    14881 cttcattttc ttcattcggg ttatcctccg attccggttc tgccgccgat gcggccggtt
    14941 ttacgtcggc aatgattcct tttcctgcca acatctggtt ttcctgttcg atttgggtaa
    15001 taacatcttc aaaatccaaa cccatttccg cgcaaatatc gcggcgggat ttgactgcca
    15061 gcgccaccgc ctctttatgc gcattaatat ctttcagcgg gtcaacccac gaccagcggc
    15121 gcccctgcca gttgcaggcc ttgaacttgt ccagcttgcc ggcaggcagc gatttgccgg
    15181 acggcatctt gattgcgccc attagcaacg ccgcctcaat ccagcgatcg aatacgtcat
    15241 acaagaacgc gtcggcaaac cagttttgca acgtcatcca tgtgtcgcgt tcctccagcg
    15301 tcccgctgcg gatactggaa aagttcacgc cctcaaggtc gttcgccaag ctgtgataag
    15361 ccacgttcaa accgctggcg ataccgcgca ggctggcttt gacgaatgcg tcgtaattgg
    15421 cgtgcgggta atccggatca aacggcgtaa agtcgtaacc ctgcggcagt tcgtgaaacg
    15481 tacccggctc gaccgaatcg attaaatcga tgctgccgcg cccgttatcg acctcttgcc
    15541 cgtcaatcgg cggcatgaag ttgtcggcgt cttccgtctg tttgaaaaag cccattttcg
    15601 atgcaccgac acgggcagcg ataatggccg cttcctgata gcccgacagg ttttgcaggc
    15661 cgatgatggc cgaagccacc cacggaaagc cgcgccgctg ctctgggcgg tcatgcagga
    15721 aaatatggct gatttgctct gccggcaccc gttcgcgtaa attgcccgta ttcgtctgtc
    15781 cgtatgattc gcccggatgt gccgtccgca accagtacgc tacaggacgg gaatagctgt
    15841 ttaactcaac acccatgcgc acggcgttac gcccgttttg ctgcggaacg ttgtatcccg
    15901 tatccaaacg gtcaatatcc agcacctgca acgcatagcc gtaatcattg tcaaaccctg
    15961 aaatatgacg aatcagcact tcgccgtccc gcgccacgct gcgaatcagc agccgttgca
    16021 ggtcggtaaa agacatctgg ccggtaacgt cgcacacgcc gcgccgcgcc cagcgggaaa
    16081 acgctgcttc aatggccttg ttcgccaagg catcgggctt gtcggcgtta tccagcaaaa
    16141 cacgcatttg cagggcaaaa ccgtcgcgcc cgataacgtt gctttcgacc atattcaaaa
    16201 acttgcgcat gtagtcgttg tcgcgtgcaa ggctgcgggc gcgagtgcgc aggcggtcaa
    16261 ggtctgaccg cgccaaagcg tccgccgacc agttttgcgg ctgccacgaa gccagcgacc
    16321 cgaccggacg cgcccctgca aagttgcggc gggctgtttt cgggacggct tcccgcctgc
    16381 cgaacaaacg cgcaaaaaaa ccgcgctttt tcggcgcggc atttgattgt tttgccatag
    16441 ctcatcctaa aatcgcgtga tgatacggcc tgaacggcgc ggggaaacac ccgccgccgc
    16501 ttccagccgc ttgatttcct gctgccagaa ccgtatctgc ttcaacaaat cggcaaggct
    16561 ggaaaactcc atttcgcggt ctttgatttt gtaccgcttg gtcatgcccc tgcctgccgc
    16621 atgttccttg taggcttttc gaaggctgtt cagaatctct cgcgcttctt caagttctac
    16681 cgcatagtca tgtccgctca tcgccgtatc ccttcaacag ttcggaaaca ccgcacattg
    16741 ccgcaagcag actgtcttca gattcaaaaa atgcatgata gagaatttct tccagtacat
    16801 gctgcatcag gtcatccaat tcgcattttt ccagaccgtc cattaactcc gcatattctt
    16861 cgggttccaa cagatgcatt acggcacaag aatcaagctg ccgctgcaaa taccatcgcg
    16921 cctttttcaa atcctccgcg ccgttctttt cccgatgacg ccagacatac ttaaacgcat
    16981 tacccatgca gaaattcagc cgttcggtaa attcaatgca ttccacggcg cggcttcgat
    17041 agtgcggcgg ggtgatgttg tcttgctttt ccattttcag acggcctctt ttgcataaaa
    17101 tttactgacg ccttgctcaa aacgctcttc caccaagcct tcctcaatca gcgtatcaat
    17161 atcgcggcgg gttaaaaaat ggcacagagc atggcacaat ggcacaaatt tatggcacaa
    17221 ccaaaaatcc aaactacaaa acgcgcccga caactctgcc gcacgcgttt tgaaaacatc
    17281 atacatatgt cagactcaat cgtcaaactt cttcaccgcc gcgatacctc caaccagcgc
    17341 tgcggaatcg taaaacctca ttgcccaggg catccatttc cggcaatccg tccgccgctt
    17401 gccagccagc cggcttaaaa atccagtttt gacacgatac gtcgccatct ttatttttcg
    17461 tccgccacca gcgccgcgcc cgcggcatct tctcagcaat gtgttggaga aactttgttt
    17521 taccagtttc ccgctcgccg ttttgcctgc accactcttt gtaaaccaac caaaggtcgt
    17581 ctgtaatgca acagcaaaac ggcaggtctt taataccacc gccagcccac tccgaataaa
    17641 aggtttgcca agcatatcgg ccataggaaa tgacgttttg tttcgcggca gtcatcggcg
    17701 gcggcgtgtt tggatcgaat cttacaggct cttccagttt gattacctcg tatccttttt
    17761 cgttttctgc tccctgttgc ccgtaagtca gataaaaatt ccaacacagc aggaaactgt
    17821 aaaacaaacg gacaccatcg ttgccgatac agtctaaaac ttcatcgcgc aattttttgt
    17881 ccaacttttt ctcaggccat acaacgaaat aacgccggtc gttttcctca attttgaaag
    17941 gatgagattc attacgcaaa aacacacaat tcatatggtt tgcttcttcg taagcatcct
    18001 ggaatttccg ctcgacgcgc atcgtcttgc cggtaatcat atgtttttgg ctgcctgttt
    18061 tattgtattt ttgctgattt gtgtaaacct cttcaaaaat accgaacatt ttgcctgatc
    18121 ggtttgccgt atagttgctt tccaagtcct cttgaccata tgttgcgccg tattcgccgt
    18181 acatcggctt cacgatttcc tcgaaaaaga aagatttacc cgcaccgtgt atatgaccgt
    18241 gcatgacaac ggcggtcgtc atcttcgcgc ctacgttttg taacggataa gccagccaat
    18301 tcaaaatgta ttcgacctgt tggtaatccc cattacataa gtgattaatc aaattcagaa
    18361 tcggcttgat ttcagggaat aatctataca attcgtagtt tgtttcgggc atatcctctt
    18421 tgggaatcgg gaatttcggt tcaatcggca tgcctcggaa gatgttgata tagtcgtcag
    18481 acaacacccg accaggctca aagaccaatc ggtcgaaccg ttttacaatt cggctcgggc
    18541 tattgaccca aattttaaaa ccatcaggcg tcatcgacag acgagccgcc ttttcaggga
    18601 caacgcgcca tagtttgttg tcccaaatcg atgccgaacc atcaagataa atgaaacgct
    18661 ccatcatatc ttgcgtgtca tcatctttta ccgcctgaat caatgcgtag tcttttttca
    18721 gacggttgat ttcgtacata gtcagcaccg gcgcacggcc ccattcatac caagcatcga
    18781 caacgtcttt gccgaaatga acagtcattg cctttcgggt atatgtaacc cctgtttttc
    18841 tgttcacgcc gtggtcttta ccttcaacca gcccaaattc tgatttcagc ttttgcaatt
    18901 tggccaaata ttcgtcatcc acatcccccg cacccccttg attgcctgtt gcttcgagag
    18961 attcatgcaa ctgctcagat tccggctcca tatcttgaat gggtgcggga gagttttcag
    19021 acggcatttc cgcttccggc acatactcaa ccaaccaatc cgccgcgccc atccagctca
    19081 acacttcgga agggtcgcag atgcgcccgc cgtctgcaat cgcatcggca atatcgaaac
    19141 catgaggcca tatccctgga gcaggaatat tgaccaacca aacacggcaa ttctgctttg
    19201 tcagtacctc agcaatacca agcatcgcct tcattccgcc ctgctcattg cgcggcagaa
    19261 acggtttgga ttcagggtca acaccatcat cgcgatcttt ctttgttagc ttttggcgca
    19321 gcgaatcgca atcaggccac aagacgacat cacgatcaac gaccgccgac caatccgact
    19381 tatcccagtt attacaaccg ccatgccacg tcaggacggc atagttataa ccctgagcat
    19441 ctgccgcgtt cttacatttc tccccctcga caaccaaaac cggtaatgat gggtcagcaa
    19501 ccaaagaatc aagaccgtac aatgggcgcg ggttatccca acaacgccaa cgccacatct
    19561 tttcgcctgt tttcttatgc tcgcaaaacg tataaggcag atcgattttt ccgccgtctg
    19621 attttctaaa tcgggcaaca ccgcccaaaa ccgcgctgga gctatcgcgg aaaacagatt
    19681 taaaaatcag gtcgtctgat tttctaaatc cattcccaaa gctcatagaa ttcagcgcat
    19741 attcaggcac aggaacgatt ggcttccaca aatcacgctc ctcctccttt actggagcag
    19801 gaggaggtaa cagcttcgga gccttatcta ccaaatcccc atgcgccaac ccaagcaagg
    19861 aggcaacttt gtctctagcc tcgttaaaac tatacccgca aacatccatc aacaaatcaa
    19921 acggactgcc gccttccggc ttgcattgat tgcaaatcca aacaccactc ccgcgacttg
    19981 aaccgctacc gtccgtaaac ctgaatcggt cggagccgcc gcatgacgga caggggtgat
    20041 gttttttatc taaaatttcg gacggtatgc cgcacgcttc taaaacctca cgccaccggt
    20101 catgcgcggc ggcattaatt tcggcttttg ttgcatattt tttattcatt ttcagtcatc
    20161 ctttacgttt tttaggcgta aaaaataccc acccacgcac acaagaatca ctctggagag
    20221 ggcgggctaa acactgattg cgcgattaat cagtacaggg taattaaatc tgtaacatcc
    20281 cttgagggat aaataacggc tcagtccaca agccgatgtg cagtatcgga caggctgctt
    20341 tcgccaacac gaggctttgt gcgatgcttc gcgccttagc tgcctcctct ttttcgcgct
    20401 cggcgtccat caatgccgcc atgactgcct gcttttgctc aaattctttt tcacggcgct
    20461 tgtacagcaa atcttgctga tagacgttgt ggcatcggcg gcatcggggc gcgtattttt
    20521 gcagcccggt ttttctgtca aagccgtttt tcggaaacgc cgttatcggc ttaatctctc
    20581 cgcactcctt acacgttttt gttcgattca tcgagaactc caatattaaa aattatcgtc
    20641 atcaatgcgg cttccccagc ccacagcgag ttttccacga caggcttttt ttatttgggt
    20701 tggtcgtctg gtttctttga gttcaaaaac aggtcgggat acttaagttt tattcgtgca
    20761 ggtatcccgc gcttcgtcca attgaaaacg cattgagggc tattccctgt tattcgacca
    20821 acttccgcgt aactgccgat tgattgcaac aggcgtttgt cttcattgac tcttttatcc
    20881 ataaataaac caaatgttta aatctaatgc taatattaaa cactatgttt agataaaaat
    20941 caagtcttgt gtaacaacat tttgtttaaa tatgggagaa taattaagcc aaccgcgaat
    21001 aggattaaaa atgacaatgc acgagacaac tgacagactt tttgagatag ccaaagagca
    21061 gggagtttta aagccggctg acatagcaga gcgtctgaat atcagccaac aggctttgaa
    21121 aaactgggaa agtcgtggca tagcggcaaa ggcgctgcct gaagtagcaa aagcattcgg
    21181 tgtatctgaa acatggctga gaacaggtga aggcagccga accgcgcccg tccttattga
    21241 ccccgaccta ccccacgaag tcaaagacat ccaccgcccg atgacgtgga gcagcaacga
    21301 cccgctgccc gacgatgatt atgttttcgt cccctacctc aaagagagct gcttcaaagg
    21361 cggagtaggc acgtatgaaa tccccgatta caacggctac cgcctgccgt tcggcaaatc
    21421 cacgcttaaa cgcaaaggca tcaatcccga caacgtgttt tgctgcaccc tgaccggcga
    21481 cagtatggag gaaaaaatcg cagaagacgc ggcaattgcc gtagatacgg gcgaaaccgc
    21541 catacgcgac ggcaaaatct acgccttcgc ccaggacggt atgttccgcg tgaagtacct
    21601 gatacggcag cccggcaaca gcgttctgat acgcagccac aacagcggtt tctatggcga
    21661 cgaaaacgcc cccttggaca gcctgaccgt tatcggcagg gtattttggt ggagcgtgct
    21721 ggattgaaaa atgttgctat tacgacgaga taatattgat agggctttca agattgtcaa
    21781 aaataggcgt tttgattccc cttggtggcc tggtgagtac gatgccggta tgaatttcct
    21841 tggcgtacaa ggagagttga aggttcacga gctgcatcac agaacagcaa ccctgtgctt
    21901 tgagtggctt ggcgaagtat ctgcgccgcg cagaaaagag aactacaaag acctcaagcc
    21961 aaatgtattg tatgactttg atggttccgg taagcatttt gccaaccctg acgcaaggta
    22021 tctattgccc gtcggttcaa gcggcttaat cttaaaacat atccaaatcg atgacgaaga
    22081 cactctgttg cggctatggt gcgcacgaaa tatcccaatg ccacaccggc tatctaaaat
    22141 cccgatgctt cggcagtatt atctaagcaa ggcatggcac gaaatttatg ctatcaacca
    22201 acacctaagg aaaacgaagc tgatagttga tgtggcatac ggccccacag attaaacaac
    22261 ccgcccgcat tatgcgggct tttttcatgc cccgccgaac ctgaaaacaa cacaaaaccg
    22321 acaaagccgc cttaataggc ggtttttttg cgcctgcgaa atctcaaaaa taaattcttt
    22381 taataatcaa aatgtttata aatcttaaac agattgttgc atattattaa acgctatgtt
    22441 taaatacacc catcgaaaca aacaacagcc acatggagta ccgacatgga aaccatcacc
    22501 atccctaaag aaaatttcga cgctttgcta aatctcgcaa gaaccgccgt cgccctgaac
    22561 gaaaatttta ttccaaaaat aaattacgga gcatccacca taccagcagc cggctttgcc
    22621 gcacttaacg agtttggaat agctcgctcc accgtggaat ctttggaaat tctcaaaatt
    22681 aaataacctc ctcggtattg tcatcccgtg ataccggcaa cctaactaac ccctctttaa
    22741 aggaaaatca aaaatggaaa cccaagaaaa gaaatttgtt cctgcctcca agcaaatacc
    22801 gaccgtaaat agcggctggt tagaccgtga attaggcgaa gccatgagtg aagcggtgcg
    22861 tgcctgcctt gctcacggta aacagtcaga aattactgtc aagctcaaaa tccaacccca
    22921 aaacatccaa agcggaacgg taaaaatcag ccacgatgta gcaaccaaac tgcccaaaga
    22981 aaaacgcgaa ggcggcatcg tctttgctac acctgacggc aacatccaag ccgacgatcc
    23041 ggcacaagga aaattgaaac tcaaacaagt tcccaatacg tcaagcacgt tgaaaatggt
    23101 cagatccaac taactcaaac ccaaaaggaa atctaaaatg gaaacccaag aaaacatgat
    23161 taaaaccgcc ttacaagcag ctcaaaaacc tttttttgag tttgcaccga acaatactct
    23221 gcttgtattc acaccagacc aagacggtgg ctggagatac aaatcacacc ccgaattgat
    23281 gcaaaacccg taccgcaagt gcggcaaatt cctcatgcac gataccgcca gtcttattaa
    23341 gtttgtacaa aaacacaaac aggacggcac acaaatctac attgatgccg atttcaaatc
    23401 agggcgtatt gatgtcaccg ccgtcatcaa cggccatacg caacaagctc ctggctggct
    23461 cgatttttat gccgtataca ccccgcaaca cactacatcg gcatccaact ggctgaacaa
    23521 caatgcgcac cgcatgaatc agatggagtt ttcccatttc ctgaccaaca atgcccgcaa
    23581 catcgtatcc aaaaatcccg gtaacgaaaa ttccgtttac ccgaccgccg ccgaagtatt
    23641 ggatttcgcg ctcaatctcg agtacaccga aaaaaccacc ttcaaacaag gctatcgtga
    23701 acaggacggc agaatcaact ttaccttcca atccgaagac tcagggcaaa cagagaaaaa
    23761 cctcaaaatg tttgaacgtt tcggcatcga gttcaccccg catcaaggcg gtgcatccta
    23821 ctttgttgaa gccctgttga aattccgcat cgacaaaaac agcggcgcac tcgtcctgtg
    23881 gtacgaattg cagcaaatcg atgctgtaat cgaacaagcg gcaaaagaca tttccgaagc
    23941 cgtacaaaaa gccttccctg aaatcgacat ctatttcggc ataatcgcct aaaaaaaaag
    24001 gtcgtctgaa attgtgaacc ttcagacgat ctccacaaaa acccaactcc gaaagacccc
    24061 aaaatgacca tcacagaaga atactccatc gacatccgcg taaccgcaga acaggggaaa
    24121 aacgactacg gctaccccac cgaacgctac ggatgcgaca tcatcaacac agacggggag
    24181 ctgcttgtcg gcatagagcc agagtacaaa accccctttg ccgccgtcag aaaggcgctc
    24241 atctgtctgg caaaagacaa cctcaaaaac gctgcctgat attaagccgt tacccccaat
    24301 aacgaggaat caaaatgaaa tacttgatcc gcaccgcctt actcgcagtc gtagccgccg
    24361 gcatctacgc ctgccaaccg caatcagaag ccgcagtgca agtcaaggct gaaaacagcc
    24421 tgaccgctat gcgcttagcc gtcgccgaca aacaggcaga gattgacggg ttgaacgccc
    24481 aaatcgacgc cgaaatcaga caacgcgaag ccgaagaatt gaaagactac cgatggatac
    24541 acggcgacgc ggaagtgccg gagctggaaa aatgaacttt caggaagaaa acgagaacat
    24601 caagaatgac atagagatta atctcttgca ttccgctgaa gccgacagcg tggaggcaat
    24661 gatggacttg gcggtttacg ggctggcggc gatggttgcc aaacacaaca aaacgcgcgg
    24721 cgagcagctt gtgttacagg cgattgccgg tcatatgcgg aagcttttgg ccgcgcttcc
    24781 cgaaaccgag tcattggtgc agaccgggaa ggtttaccgc cgacttgaag atttgatttt
    24841 taaaacgtat ttgccggagc agtaaagatg aacacgaatc aagatacgat tccatttggc
    24901 ggcaaccttg tgatttgctg ttcgacggga aacggcgggg acgggcggtt ttcttgtctg
    24961 attaccgatc agataccgga tttaaatctt gtcagaagcg ggcaggcgtt cccgatgttc
    25021 atttatgagt acgaaactag agaaaaaagg aaagcctgat ggacacccta ttaaaaatca
    25081 tcatcgcgct gtcattttcc ggcgctgagg cattggcggt atggcttttc atcacggccg
    25141 ccgatgccgt attacgccgc ttcccgaaac cgagtcatta caggagagta aaaaaatgaa
    25201 catcaacgaa ctgggcgcaa ggatagaccg cccgaccatc cgcgaactga ttgcctacgc
    25261 aacctgccgc aaccgcccca tttcaaactc gacactgctg cgtatggaaa aagacggacg
    25321 gataccgtgc cgtctgaaaa ccccactcac atcccccgta tgggacaccc gcgaagtttt
    25381 ggaagccctg gggttacagc aataaaaaaa cgcccgatta gggcgttttc acattcagac
    25441 ggcttttgta ttccctactg catcaaaccg acgacaggtt gcggatttcg ggcagcatcg
    25501 ggcggatttt tgccgcgtgt tccgcgtcgg cgtgtgcgtt taaggcttcg agggcgtttg
    25561 cggcggcttt gaggcggctg cgtgtttccg cccagaccgt ccacatcgtt accgcctgtt
    25621 tgcagccgag ctgcttcagc ggcagggaaa cgtctctgcc catttggatg gcccacgcgc
    25681 cgtatctgac ggcaacggcg aggtcgtaca gggcgttgcc gctgatgggc agggcaggtt
    25741 gcggtgcggg taacggttcg cggtcgagga cttcgccgta cagcacgccg ttttcaactg
    25801 ccacgcggct catcacgctt aaaacttcgg ggaactgttc ggcaggtact tctttgtagc
    25861 tgcatccgaa cttgcttttg acggcagacc aaagggtaat ggcgatacgc gcctgcgctt
    25921 ctttgggtgc ggatttggtc agggcgttgt gcagtttttt gacggcttcg atttgttcgc
    25981 cggtcaagcc gtccggaagg gcgcgggtgg gtttgggggt aactgttttt atgccgtctg
    26041 aaattttgcc gttaagtaaa atttcaatct gctcatcgca ccaaaccgca aatttcggat
    26101 taagccaacg ggcaaagtga atagcgagtt tgggatgcag ccatgtgcct tgctcactgc
    26161 caccacgctt cacgataact atttgatttg cttccgttag gatttttctc ctaacgctta
    26221 aattctcagc aagtgcagag atatattgtt gagtttgttc actttttagg tagtctttag
    26281 gtaacttgcc aaagtgagat gcaatggcgg ttgcatttaa aaaaccgtct tgacggaaag
    26341 atacaggggt gttaccaaaa ttgagaacag atacgttcat gatagtttcc tagtttagtt
    26401 tcgaaagacc caaatgggtg gtcgggaggt tcgaaaacct actagaaaag gccggcgtta
    26461 ttccccttgc gggtattgta ttccgcaccc tcccgaccat aggaaactag ttcggatata
    26521 tcgaaacaaa caaccaaaca agaaaactta tggacgtaaa aaattcacgc tgacggggtg
    26581 aatgccgttc tagtagaggt tttcgacgcc tcgtgtttcg taatatatcc aatctttcta
    26641 ttttctgcaa gcataaaaaa gccgccatgt ggcggatagg gtcgcggtct tcaacagcat
    26701 gtaggattta ggaatgccca aattgggcgc aggggtgttg aaaacacgca aacagacgcg
    26761 ccgccagcct tacgggtagg cgcaccccca cataggagta aatcgatttg atacgtatag
    26821 acgtaaaaaa ttccactcta tcgtgttgga tatacgctgt ttgtcaggtg ttttcaagca
    26881 ccgtgggaaa tattatatag agttatggaa atgtgtcaag agaattagcc caaatgggcg
    26941 gacgcgcggt taagaaccca cataggaggg cggacttatt cccctttcgg gtcttgtatt
    27001 cgtcgcccac gcggtcatag aaacttcctg ctatcgaaac aaacaacaag gaaagaaaac
    27061 tatagacatg aaaaaatcac attgacggag tgattgccgc tgtagtgtgg tttctgacgc
    27121 cacgaacagg aatataaaac aaaacccctg cacatgcaag gggtttccca aaaaaccgta
    27181 ggcggcaaac tgaaaggccg tctgaatttc agacggccta tgttgcggcg gatgttaagc
    27241 tattaagcta tttggttttc agaggctttt tcttccattt tcgggctacg gcttcaaatt
    27301 cttgaaaggc cgtcggtgcg ctcctgatac ggcgtatttc aaaaatgaag ctgcttaggt
    27361 tgtcccagtc acgtaacagc atggagtatt cgaaatcttt atacattttc tcgtgcagtg
    27421 ctccgcccaa tacgcccgca cagacaaatt cgcgctggtt taaaactgtc agtatggctt
    27481 cacggtcttt cttcttgtcc ggggtatccg atgtataggt ggcgagtatg catccgtctg
    27541 tttttgccag cccgtttact atggttatgg cttcttgaag ggcggcattg ttacgttcgg
    27601 ccatgatcat gtcgatggtg gcgcgctttt tggcgttttc tgtattcctc atgatgccgt
    27661 aagcggcaac aaaaacgccg ataacagtca atatcggcgt tgctatctgt attaggttgt
    27721 cagtcattgc cgtcccagcc gtctgaaagg cggaaatcac ggctttccga ataaatatgt
    27781 cccattgtca tccccttaag ctgatgttga aaataaatcc ttgcgtcgga atatacggca
    27841 ttgcttgact attttcaaga ttctgtacct cgtgaaccgg actatacaaa aaaaccgcct
    27901 tcgtgtaaag cggttcttaa atcgcccgtt aaggcggaca caacgatagc caaactggaa
    27961 caggaggcgg caaaggcggg tttgtccaaa acagccttga ttgaaaggct gattggggat
    28021 ttgtaggcgt ttgcagatac gacaaggggc gcattccgcg ccccatcaca cgcacggacg
    28081 gcttgtcatc gcctgtcgcc cgtgcgcggc tgcccatatt tcagaacagt cttgcaagcc
    28141 cctttaaggg aacggtttta ttctagtaca gtttgaatgc cttggcaacg gctgttttaa
    28201 tcgcctgaaa atcctcttca ctgattattg gaatgcagcg gtcgcgccct ttgggtttgt
    28261 atcggtctaa tcgtgccaat ccgactgttg ccgtcatgtc gcattttgcc caacattgga
    28321 tgtgcggctt gtccggtaag gggtttccac tcattttgtg gtggtagtcc gccaaaggga
    28381 caggttctgt gctgcttaag ggtacgaccg ttaccagttt gccgttgtgc ctgtttcgcg
    28441 ctatgacgac gacagggcgt ttcttgacca tttccggttc ttcataaccg cgaaagtcgc
    28501 acatgataac cgaacgttcc cttggttgga attttaaagg cattagccgc tcctgacgat
    28561 gacaggctgc tattatagca gttcttacac aaaaaaccgc cttcgtgtaa agcggttgca
    28621 aaaaagcctt ccaataaaaa tgcggaaacg gttttttatt tgcgtttccg cataatacaa
    28681 acacacacac aaaaaaatca ttttccgttt ttggcaagcc tcaaaacatc attcccccac
    28741 cgttccagcg catccttacg ctcctcccaa agctccgagc ggttgtaagc cgtttgcgtc
    28801 ctcgtcccca acgaatgggc aagcagcttt tccgccacat cattatccac cttcagaacc
    28861 tcgcgcaaat aagtacgcgc aagcaagcgc aaaccgtgcg ccgtcgtatc cagccccgcc
    28921 cgctgcatcg ccacgcgcgg actttcctta ttgatatgcc cttgaaaatt cacactttca
    28981 aacacaaacc gcccgttcac attcaacgcc cgcgcctgat ccaacaccca ctgcatcgcc
    29041 cgcgacagcg gcacatcatg cccccgccgc tcctttttcc gttcggcggg gatacgccaa
    29101 acctccccgt ccaactccga ccactccatc aacgccgcct cctgaatccg cgtcattgtc
    29161 aacagcagcc aatagataca aagccgcgtt acaggatgga tacgcaccct gcccgcataa
    29221 accgcaaatt cattgcgctg ctccaaaaaa tcaatcaggc gcggcaattc agacggactc
    29281 aacgccgcca tattccccgt tttcgcccgt tcaaacacac ccgaaccgat ttgcgcgaca
    29341 gggttgattt ccatcattcc cgaaccgacc gcaaacgcaa acaccatctt cagactgttt
    29401 ttcgttttgc gcaacgtatc aacgataccg cgcgcctcca tcacacgcag acagccgacc
    29461 acatccgccg tcctgacttg acgaatatca agattgccga tagccggaaa aacccaccgc
    29521 tcaaaattcc gcataacctg tccggcatac ttttcagacc gccccttcga ccaacgcaca
    29581 aaccaatcac gcgccacctt ctcaaaagca aaatccgccc gcaccttctt attgacgaca
    29641 ttttccccgt gcgcccgttt tcggcgcacc tcctcccgcc attcccgcgc atcggccagc
    29701 gaaaaatcag gataccgccc cagcgaaatt gtctgctgcc ttccatcctg cacaaacgac
    29761 agcttccaac tccgcccgcc cgtcgggtag acccacaaag ccagcccgcc cccgtccgac
    29821 aacttataca gcttatcgcg cggcttcgca tttttaacct gatttgccga cagcggcgta
    29881 atgattttcg ccattatggt aatttcccat ttatccaaaa attaccatac acattaccat
    29941 aaaaaaaatg caataagaag atgccgcaag attgcaaatg atttactttg acgcgttttg
    30001 atagggcaaa ccgatgattt ttatatggta attgacacaa cttgaacaaa aaagaaccgc
    30061 cctgaatcag ggcggttttg ttttctggcg gaaacggtgg gattcgaacc cacggaggat
    30121 ttgcaccctc agcggatttc gagtccgctg cattcagcct ctctgccacg tttccgataa
    30181 tgaagtaaaa ccaaataaaa atgccgatgc tttatgctac ttcattttta tttggttttt
    30241 gacctcaagg attggcggaa gcggtgagat tcgaactcac ggagggctat caaccctcga
    30301 cggttttcaa gaccgttgca ttaaaccgct ctgccacgct tccatcttct tgaagaagac
    30361 gaataataat gaagtttata cattttgcca agcacttttt tcggaaaata cttaatttat
    30421 tgttttcact ttatttgttc cgaacgcata aacacccatt cactttcatt ggaagccgcg
    30481 tcattgaatg cgtagccttc gtaattaaaa tttttcagca tttcaggatc ggtaatattg
    30541 tgttctgccg catagcgcac cattaatccg cgcgcgcgct tggcatagaa actgatgatt
    30601 ttatatttac cgtttttttc gtctttaaat atcggggtaa tcagccgcgc ccgaagtgtt
    30661 ttcgtgttga cggacttgaa atattcctgt gaggcaaggt tgacaagcgt attgctgcct
    30721 gcttgggcaa gcgtgtcatt taaaaggttg gtaatgatgt ctccccaaaa ctcatacaaa
    30781 ttcttcccgc gcaaattggc aaatgccgtc cccatttcca aacggtaagg ctgtatcagg
    30841 tccagtggac gcagaaggcc gtacaggccg gacagcaggc gtacgcgccc ttgcagatat
    30901 tgtatttgat cggtattcaa tgtgtttgca tccatacctt cgtaaacatc gccgttgaac
    30961 ataaagaccg cctgtttggc gttttccggc gtaaacggcg tgttccattc tgcattgcgc
    31021 tgcgcgttta agagggcaat tttgtcggaa acgtgcatca gttcggcaat ctgttgcggc
    31081 gcaagctcgc gcaactgctg cattagaatg tcggactctg ccagcaggtc gggttgggta
    31141 aactcgctga caggggcagg gtctttttca ttaaggttct tcgcagggga aaggacaaaa
    31201 aacataatct gctcggtatt gtgtcaaaca cggggatttt aagtttaaac gtgtttgcgg
    31261 gcaagccgat taaaagtgaa gtatagtgga ttaaatttaa accagtacag cgttgcctcg
    31321 ccttgccgta ctatctgtac tgtcttcggc ttcgtcgcct tgtcctgatt tttgttaatc
    31381 cactataaat accgttacag caaattcaac cgtagaatat tgaaaaatgc cgtctgaaca
    31441 aaagttcaga cggcatttcc gattccttac cgatacagtg aaactatgtc ttccgcagct
    31501 ttgacacaag ttgcgacatc ggcaaccaaa gcgatacgga cataaccttc cccgggattg
    31561 ccctgttcgg tatcccgtgc caaaaagcgt ccgggtaata cttggatagc ggctttttgc
    31621 cataaattgc gtgcaaatgc caaatcgtcg ccatcgggga ctttcaacca gatgtaaaac
    31681 gaggcatccg gtaatttgac gtcaaatacc tgttgcagga taggaataac ccattcaaac
    31741 ttttcctgat acaggcggcg gttgtcaata acgtgctgtt cgtcattcca ggcggcaata
    31801 ctggcacgct gtacgggaat gctcattgcg ctgccgtgat aggttctgta aagcagaaaa
    31861 tttttaagca gttcggcatc gccggcgaca aaaccggaac gcaggcctgg aacattggac
    31921 cgcttggaca aactggtgaa cataagcagt ttttgcctgc ttcgccccaa ctgtgcagcg
    31981 gcctgcaggc agcccaaagg cttgttgccg tcgaaataga tttcggaata gcattcgtcc
    32041 gaggcaataa tgaaaccata tttgtcctgc aaatcaaaaa cttctttcca tccgtccaaa
    32101 tccagcacgc tgccgctggg gttgttgggc gagcagacga acaccagttt ggtgcgtttc
    32161 caaacctctt cggaaatact acgccaatcg gggttgaaag acggcgcggg gcaattggca
    32221 aaatggattt caccgccgcc caaaagtgtc gcaccttcat aaatctgata aaagggattc
    32281 gggctgacga ttacgggttt gaggccgtct gaaacagggt tcaacacggt ttggacaaaa
    32341 gaaaacaacg cctccctact tcccaaaacc ggcagaattt cggtgtcgga attcagcgtc
    32401 aacccatcat aacggcgttt taaccagttc gcacacgcct gacgcagttc aggcaaaccg
    32461 gccgtcagcg gatatttttc caactcgtgc aatgaggcgg tcagcgcatc cgtaatgact
    32521 ttcggtgtcg gatgtttcgg ttcgccgata tgcagtggga cggcttccag accttcgggg
    32581 gcggaaatgc cctgcatcgc ttcacgcagt cgggcaaagg gatagggttt gagttggttg
    32641 agtaaggtat tcatgatttt ctccggttgt cggtatatcg gaagatatgc cgtctgaacc
    32701 tgacgtgccc ttcagacggc attttatatt caatgcaaac ccgtactgcc gaagccgccc
    32761 tcaccccggc tgcttccgac aaattcctcg acacgtttga aacgcgcctg cacgattggc
    32821 acgacaacca tctgcgcgat acgctcaaac ggtttgacag taaacggttc gctgcttctg
    32881 ttccataacg acaccttcaa ttccccttga taatcggagt caatcaaacc gaccaaattg
    32941 cccaaaacaa tgccgtgttt atgccccaag ccggaacggg gcagcaaaac ggcggcatat
    33001 gcgggattcg ccaaataaat tgccaaaccc gtcggcacaa gaaacgtttc acccggctgc
    33061 aaaacgactt cctcatccaa acaggcgcgc aaatctaaac ctgcagaacc cttcgttgca
    33121 tagacaggga caacatccgc catccgttcg tccaatactt tcatttctac ttcaatattc
    33181 atcgtcttgc tcctgcatac ggccggtttc aaagatggga gattatacac aagccgtgtt
    33241 tacgctgaaa cacagcaaat aggttttgtt ttgaaatact ccgtctgtgt ataatgcccg
    33301 accttcttac atatcaatat taatatgaca cacataagct cgccccgtaa cgaatttata
    33361 cgcggcatca aagaaagttc gcccatgctg atcgggcttt tgccttgggc attaatactc
    33421 ggtatgcagg gtggacaaaa aggcatgagc tggctggaaa tgttgttgat gaccggtatg
    33481 aacttcgccg gcggctccga gtttgccacg gtcaacctgt gggcggaacc tctgccgata
    33541 ctgcttatcg ccaccgtaac ctttatgatt aattctcggc atatcctgat gggggcggca
    33601 cttgccccgc acctgaaaga aataccgctg aaaaaagccg tgcccgcact gttttttatg
    33661 tgtgatgaaa gctgggcgat ggcattctcc gaaatccaaa aacggaaagc agccggtttg
    33721 cccgcattca atatgcct
//
LOCUS       DAWEWP010000003        32086 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWEWP010000003 DAWEWP010000000
VERSION     DAWEWP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32086
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.003"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(226..549)
                     /locus_tag="WGN53_000069"
     CDS             complement(226..549)
                     /locus_tag="WGN53_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ0139119.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(629..1285)
                     /locus_tag="WGN53_000070"
     CDS             complement(629..1285)
                     /locus_tag="WGN53_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ0139120.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1387..1890)
                     /locus_tag="WGN53_000071"
     CDS             complement(1387..1890)
                     /locus_tag="WGN53_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ0139121.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            2074..2847
                     /locus_tag="WGN53_000072"
     CDS             2074..2847
                     /locus_tag="WGN53_000072"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ0139122.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2854..3204
                     /gene="secG"
                     /locus_tag="WGN53_000073"
     CDS             2854..3204
                     /gene="secG"
                     /locus_tag="WGN53_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ0139123.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKYGLDFSNVQQTQQAPKPI
                     SNTEPSAPVPQQQK"
     gene            3218..3303
                     /locus_tag="WGN53_000074"
     tRNA            3218..3303
                     /locus_tag="WGN53_000074"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3252..3254,aa:Leu,seq:gag)
     gene            4104..4472
                     /locus_tag="WGN53_000075"
     CDS             4104..4472
                     /locus_tag="WGN53_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0139124.1"
                     /translation="MRIFKNQWIVKFAKKHKISDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4469..4780
                     /locus_tag="WGN53_000076"
     CDS             4469..4780
                     /locus_tag="WGN53_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ0139125.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5550..6104)
                     /locus_tag="WGN53_000077"
     CDS             complement(5550..6104)
                     /locus_tag="WGN53_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139126.1"
                     /translation="MIIGGIGGARTQTGLRFEERTDLRTLFEEIPGYDLRKTYDNAGY
                     EVWFNGELKAYCFKKYEFYRFLKRPGYNINWKDHLSKRLLPDNGLFIIIRDTLFIIEI
                     KFQQTPGSVDEKLQTCDFKRKQYTKLVHSLGWRVEYVYVLNDWFTKPEYKDVLDYIIS
                     VNCHYQFNTIPLRWFGLPDGETNE"
     gene            complement(6101..6910)
                     /locus_tag="WGN53_000078"
     CDS             complement(6101..6910)
                     /locus_tag="WGN53_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0139127.1"
                     /translation="MIDFDKPAEEDAIYQSRLKKIVSDDLVLYNENSLNVMRKILEKY
                     PNGCFDMIFSDPPYFLSNDGFSCQNGQMVSVNKGNWDKSKGMAADLEFYEEWLRLCYA
                     LLKPNGTIWVCGTFHNIYLIGYLMQTIGYHILNNITWEKPNPPPNLSCRFFTHSTETI
                     LWAKKNKKAKHTFHYEMMKAQNDGKQMKCVWTFPPPNKAEKTFGKHPTQKPLPLLERC
                     ILSASNIGDLIFDPFMGSGTTGVAALKHGRKFCGCELEEDFFELAKKRLEK"
     gene            complement(6903..7715)
                     /locus_tag="WGN53_000079"
     CDS             complement(6903..7715)
                     /locus_tag="WGN53_000079"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEZ0139128.1"
                     /translation="MASKPFLKWAGGKHKLVPFIEHNLPTPTRKRLIEPFCGSAALSL
                     ALDFEHYLLNDINADLIGLFRILKEEKSGFIDYARSFFISENNSDSRFYELREQFNFS
                     KNLHERSALFIYLNRHAFNGLCRYNSKGVFNVPFGRYKSPYFPQQEMEGFIQKSDRVE
                     LMCGDFQMILSQTDNTDTVYCDPPYAPLSPTASFTTYAKESFNLDNQIRLAQSAQQIA
                     PDSQGVLISNHDTEFTRDIYSFAILKTIEVQRNIAAKGSSRKKVGELLAIYD"
     gene            complement(7953..10583)
                     /locus_tag="WGN53_000080"
     CDS             complement(7953..10583)
                     /locus_tag="WGN53_000080"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ0139129.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKLLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYADKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10850..11365
                     /locus_tag="WGN53_000081"
     CDS             10850..11365
                     /locus_tag="WGN53_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0139130.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNNRGTFPTYDELDLMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEGITTCQYKIIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            11372..11815
                     /locus_tag="WGN53_000082"
     CDS             11372..11815
                     /locus_tag="WGN53_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ0139131.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11984..14041
                     /gene="ppk1"
                     /locus_tag="WGN53_000083"
     CDS             11984..14041
                     /gene="ppk1"
                     /locus_tag="WGN53_000083"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ0139132.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPIAAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            14066..14335
                     /locus_tag="WGN53_000084"
     CDS             14066..14335
                     /locus_tag="WGN53_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139133.1"
                     /translation="MPSETFPFQTAWFYSNLNRADWNQKHTFAIPAQALPLYAPNPNP
                     PHSRRHCLSDSRTKPRIRLHGSGHISNTGIVLRKTVKSASASQYI"
     gene            complement(14304..15407)
                     /locus_tag="WGN53_000085"
     CDS             complement(14304..15407)
                     /locus_tag="WGN53_000085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ0139134.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(15487..17043)
                     /gene="dnaA"
                     /locus_tag="WGN53_000086"
     CDS             complement(15487..17043)
                     /gene="dnaA"
                     /locus_tag="WGN53_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ0139135.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRHTAEPASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFLWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            17336..17470
                     /gene="rpmH"
                     /locus_tag="WGN53_000087"
     CDS             17336..17470
                     /gene="rpmH"
                     /locus_tag="WGN53_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ0139136.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            17473..17838
                     /locus_tag="WGN53_000088"
     CDS             17473..17838
                     /locus_tag="WGN53_000088"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ0139137.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRSTAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            17903..18124
                     /gene="yidD"
                     /locus_tag="WGN53_000089"
     CDS             17903..18124
                     /gene="yidD"
                     /locus_tag="WGN53_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ0139138.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPLGGHGHDPVP"
     gene            18296..19933
                     /gene="yidC"
                     /locus_tag="WGN53_000090"
     CDS             18296..19933
                     /gene="yidC"
                     /locus_tag="WGN53_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ0139139.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGRQSVCAAGECNIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(20014..20724)
                     /locus_tag="WGN53_000091"
     CDS             complement(20014..20724)
                     /locus_tag="WGN53_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ0139140.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTARA
                     HLKHLGVTTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQAFRALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQEIISQTIVQWRKR
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(20786..21376)
                     /gene="maf"
                     /locus_tag="WGN53_000092"
     CDS             complement(20786..21376)
                     /gene="maf"
                     /locus_tag="WGN53_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ0139141.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAIVLLNTVTGRMRRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            21524..22027
                     /locus_tag="WGN53_000093"
     CDS             21524..22027
                     /locus_tag="WGN53_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ0139142.1"
                     /translation="MSDPNLIDLEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            22061..22240
                     /gene="rpmF"
                     /locus_tag="WGN53_000094"
     CDS             22061..22240
                     /gene="rpmF"
                     /locus_tag="WGN53_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ0139143.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            22415..22942
                     /locus_tag="WGN53_000095"
     CDS             22415..22942
                     /locus_tag="WGN53_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ0139144.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            23030..24085
                     /gene="plsX"
                     /locus_tag="WGN53_000096"
     CDS             23030..24085
                     /gene="plsX"
                     /locus_tag="WGN53_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ0139145.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            24221..24472
                     /locus_tag="WGN53_000097"
     CDS             24221..24472
                     /locus_tag="WGN53_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0139146.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            24499..24915
                     /locus_tag="WGN53_000098"
     CDS             24499..24915
                     /locus_tag="WGN53_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139147.1"
                     /translation="MKTSTVVFGGFFMADNGERIQIPVLENPDIREINHFFSVSNFEK
                     KTGVLVFRIIPEPEFGNTELTVYFEKGYYLPMIQTILEGGDIEVKNLKTENYSGNTME
                     IWGDSYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            24935..25138
                     /locus_tag="WGN53_000099"
     CDS             24935..25138
                     /locus_tag="WGN53_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139148.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            25165..26127
                     /locus_tag="WGN53_000100"
     CDS             25165..26127
                     /locus_tag="WGN53_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ0139149.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            26174..26545
                     /locus_tag="WGN53_000101"
     CDS             26174..26545
                     /locus_tag="WGN53_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ0139150.1"
                     /translation="MKRQAFFKPIACAAFLSAISLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLILIDYFVPPELFVKLGQHL"
     gene            26571..27497
                     /gene="fabD"
                     /locus_tag="WGN53_000102"
     CDS             26571..27497
                     /gene="fabD"
                     /locus_tag="WGN53_000102"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ0139151.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAEAAQGEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIEAH"
     gene            27636..29501
                     /gene="msbA"
                     /locus_tag="WGN53_000103"
     CDS             27636..29501
                     /gene="msbA"
                     /locus_tag="WGN53_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ0139152.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            29601..31166
                     /gene="guaA"
                     /locus_tag="WGN53_000104"
     CDS             29601..31166
                     /gene="guaA"
                     /locus_tag="WGN53_000104"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ0139153.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPILGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDDTPNTLDVWMSHGDKVSKLPTGFTVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPSWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMEKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            31228..31974
                     /gene="fabG"
                     /locus_tag="WGN53_000105"
     CDS             31228..31974
                     /gene="fabG"
                     /locus_tag="WGN53_000105"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ0139154.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         8078 a         9325 c         7726 g         6957 t
ORIGIN      
        1 gcctgcacat tgaaaatgcc gcccaagcaa accttcagtt tgccagcctc gtcctagccc
       61 tctcccacgg ggagagggga ttggggtgca ggctgccttt aaggttcagg caaattttaa
      121 cttcgttgat accgcgcttt agcttcgcag aagctgcact ttcagacgac cttttggaac
      181 accacaggta cacgcattta aggaatgccg tctgaaatgc ctgcctcaat aacgcatcat
      241 gttgccgtca atctcggccg cccatgcatc gatgccgccc tgaaggttgt acaggttttc
      301 aaaacccgcg tccgccaaat acatcgccgt atgcaggctg cggataccgt ggtggcaata
      361 caccacaagc ggcacatcat ccggcagctc gttctgccgc agcggaatca gattcatcgg
      421 gatatgcagc gcatttggca gcgaacaaac cgccgtttct tcatccgtac gcacgtccaa
      481 caaacaaaac atccgccctt cgtccatcca cgccttcaat tccgccgacc caagttgcac
      541 aatatccatc gcacccccca aaaaaaaacc aagcaaaatg ccgtctgaag ccccaaaccc
      601 gctttcagac ggcatcgcaa tccgaacatc aaaactgaaa accgccaaac ggatcggcat
      661 ccttatcatc caaatgcgcc accaaggtat cgaacagcac cttctcttca aacacatcgc
      721 ccttgcgcgt aatcaaaagc gcgcgctgaa caggcttgcg gcctacgata accaccatgc
      781 gtccgccatc tttcaactgt tctttcaaca cttcaggcac aaggtttacc gcaccgccga
      841 cataaaccgc atcaaacggc gcacctgcgg aaagttcggt caacccgttg ttttgcacat
      901 aatcgatatt gtccaaaccc aagccgtcca acaccgcttt ggcgcggttt tgctgttcgg
      961 catcgatgtc gtccgacacc acacgaccag ccaattttgc caacagcgcg gtcgcatagc
     1021 ccgaacccgt gccgatttcc aaaaccgtat cgtttttcgt cagcttcaag ccctgcgcca
     1081 gccgcgccac gactttcggc tcgagcatct tatgaccgtt ggcaagcggc agcgccatat
     1141 ccgcatacgc caaaccctgc aagtcctcat cgacaaaaag ctcgcgcgga atctccgcca
     1201 aagcgtccaa aacgtcaaaa tccaatacat cccaaggacg gatttgctgt tcgaccatat
     1261 tgaaccgcgc tttttcaaaa tccatttagt gctccgtcaa ataattgttc caacaatagc
     1321 aggcattata aaaccacact cccgccgcgc caacttcggc acgcgccgcc cgcccctttc
     1381 cctcactcaa accgcctgac gcgaagcctc aaaccgcttc tccaaaatct tcgccagctc
     1441 gcccaaatac aaagcatctg tctcatcaaa ctgcgccaaa tgttcgctgt ccgcgtccaa
     1501 cacgccgata cagcggccgt ctgaaaacag cggcacgaca atctccgaac gcgacaaaga
     1561 cgaacaggca atatggtcgg gatgcgcgtc cacatccccg acgaccaccg tcccgccctt
     1621 cgcccaagcc tgaccgcaca ccccgcgacc gaacggaatc cgcgtacacg ccaaaggccc
     1681 ctgaaacggt gccaaaacca attcgtccga acgcgtatcg accaaataaa aacccaccca
     1741 aaaccaaccg aacgcctcct tcaaaaccgc cgccgtgttc gccaaattcg ccacccaatc
     1801 cgtctcgtcc gccaccacag actcaatctg cggcaacacc tcccgataaa gcgcggcctt
     1861 gtccgaagcc gaaaaatgaa gcgcgtgcat cacatctcct atagttgcat acatatcagg
     1921 cggccattat aaaacaacct gcccgaaaca acattccaaa ccgcccccgc agccgcccgc
     1981 cccaagttgc gaacccgccg catatccact aaacttcaca ttgcaacgcg ccacacgcgg
     2041 cagacagaaa aaacacgaca cggagcaaaa aagatgtatc accaaatcgg aatgtgggat
     2101 caaaaatggg tcatcggcaa ctggaaaatg aacggccggc tccagaacaa caattcgctc
     2161 atgcaccgct tccgcatcct ccccaccgcc gaacgcgtcc tcatcggact cgccgccccg
     2221 accgtttacc tgctgcaact gcacaacgcc atgcaaatcg tcttaaacaa ccgcatcctc
     2281 acctgcgccc aagacgtgag ccgcttcccc gacaacggcg cgtacaccgg cgaagtgtcc
     2341 gccgaaatgc tcgccgacac cggcacagac atcgtcctca tcggacactc cgaacgcagc
     2401 ctttatttcg gcgaaaaaaa cgaaatccaa cgccgcaaaa tggaaaacgt cctcaacgtc
     2461 ggactcatcc cgttattgtg cgtcggcgaa agcctcgaag agcgcgaagc cggcaaagaa
     2521 cacgaagtca tcgcccatca gctttccatc ctgcaagggc tggataccaa aaacatcgcc
     2581 gtcgcctacg aacccgtctg ggcgatcggc accggcaaag tcgccaccgt cgaacagatt
     2641 gccgatatgc acgcattcat ctacaaagaa atcttgtctt tgtgcggaag cgatgttaaa
     2701 atccgcgtcc tttacggcgg aagtgtgaaa gcggacaacg cggccgacat cttcgcagta
     2761 ccttatgtgg acggcgcact cgtcggcggc gcgtcattgt cgtacgactc ctttaccgcc
     2821 atcatcagtg ccgcacaaaa tgcgtagaaa aatatggaag ccttcaaaac cctaatttgg
     2881 attgttaata taatttccgc tttggccgtc atcgtgttag tattgctcca acacggcaaa
     2941 ggcgcggatg ccggcgcgac cttcggatcg ggaagcggca gcgcgcaagg cgtattcggc
     3001 tctgccggca acgctaactt cctcagccgt tcgaccgccg ttgcagcaac atttttcttt
     3061 gcaacctgca tggctatggt gtatattcac acccacacga caaaatacgg tttggacttc
     3121 agcaacgtac aacaaactca gcaagcaccc aaacccataa gcaataccga accttctgcc
     3181 cctgttcctc agcagcagaa ataacagttt ttcaaatgcc gacatggtga aattggtaga
     3241 cacgctatct tgagggggta gtggccgtag gctgtgcgag ttcaaatctc gctgtcggca
     3301 ccaacacaca aaacgcttag aaattctaag cgtttttttc gtttaatcct gtctaatgcc
     3361 gtcaaccaat cccaatcaat acaaggattt caggctgatt gttatcctgc cgtccccctt
     3421 cctgacagtg caatcccgtc caatccgccc taattgaact aacctgattt tttacagcat
     3481 ccgaaaatac cgcgagaaaa taccgtaaaa ataaagctga acgaccacca aatcaggaat
     3541 gccaagcggg aaatagcttg cggggaatac tgacgcaccg tagggaatga gggggaaacc
     3601 gtccaagatg cagagcggtt tttttttggg tttttggaaa aaacctatac tatagtggat
     3661 taaatttaaa ccggtacggc gttgcctcgc cttgccgtac cggtttaaat ttaattcact
     3721 ataaaataag aaaaagataa aaaattggta acaaatgcgg taacaaatgg taacgaatcg
     3781 gtaacaactt ttggggtttt ccggtttttc accgtcttgg cagtgggagc gtagcggaat
     3841 gaaaagccaa aacgcacgga accgcgccta ttttgagcag gaatggcggt taaaccgctt
     3901 ggttatatac ggggaatagg aagacagcga aacgcgctaa aaacgcaatt tgagccatta
     3961 aaagcgattg attaaaaaat aatcaggtta gccgccagtg tttcaggcgg cataaacgga
     4021 gaaattgcag ggtataaaaa ggcggcttgc cgtgttgtct gtctctggta taattccaag
     4081 tatcactaat caacggctac acaatgcgga tattcaaaaa ccaatggata gtgaaatttg
     4141 ccaagaaaca taaaatcagt gattccgagc tgctggaagc ggtagagcgg gcggataacg
     4201 ggctgataga cgcagattta ggcggcggtg tgattaagca gcgcatagca aggcaaggac
     4261 aaggcagaag cggcggctat cgcagcctga tattgttcaa acaggcagat agggcatttt
     4321 tcgtttacgc ctttgccaag aacgacaggg aaaacatttc ggataaagaa ctggacgttt
     4381 accgaaaagc cgccgcatat tatctgaaat acacgcgggc agagctggcg gctttgaaag
     4441 aagacggcat tatcacggag atagaatcat gaaatacaaa agcgaggcat tagccgccat
     4501 tcatgaaatg atggaagggg cttacaacat cggcgcaatc gacaaaaaga ctatgcgcgg
     4561 ctttgacaag tcctgcctga ccgaaatcaa accgttgagc ggcggagaca tcaaggcaat
     4621 cagggagaag gaggcactat cgcaagccgc tttcgccatc tatctcaacg tgggaaaaaa
     4681 tcacgtttcg gcttgggagc ggggcgttaa aaagccgagc ggcgcggcgt tgaagctgct
     4741 gaccatcgtc aaaaacaagg gtatcgaagc cattgcgtag ccgacttggc aaacggcaaa
     4801 atcagcaagt tcacaataga cacgctgctg aatatgcctg ccaaaacagg caagaccgcc
     4861 gaactgaata tcagggcgta gccgcataaa tgcccgaccg catcaaacca agccgaaacg
     4921 gcgacggtgc agacgacata gcccgacagc aaggcacggc gcagacgggc gcgaaacccg
     4981 aaacatcccc gaccgcgagg cacggggatt ttttgagccc gttgcagggg gattggattt
     5041 aagcggcgcg ggcttgaaag caaaacgggc ggggcgcaga actgtttaaa tgccgtctga
     5101 atctcaaacg atttcagacg gcattttgaa acaacggctc aaattctcga tccccttccc
     5161 ttaacgccga cgttttttat taacgcgccc cttatttctg acactttgct cataaaccgg
     5221 cataacggtc ggcaacaacc gttttagatt ttctatacgg gcattgttta cagcatcagc
     5281 ataagacacc gtactctgga ataccgccta aaccctgcgg atagttttgg atgtggtatc
     5341 gacattgtcg gcagacttgt ctaactcgac cgttttcaaa taatctttga cagatgctgc
     5401 attcagtgtc gcggaatccg cgtattttga acaaaaggtc gggctacttt atcacaatag
     5461 cagaaagatg gaaaacggcg gggattggtg aaaggtcgtc tgaaatgata ttcagacgac
     5521 cttgaacggc tgtgttttaa atatggtatt tattcatttg tttcaccatc aggcagtcca
     5581 aaccacctta agggaatcgt gttaaattgg taatggcagt taacactgat aatataatct
     5641 aaaacatctt tatattccgg ctttgtaaac caatcattca aaacatagac atattcaacc
     5701 cgccaaccta aagagtgaac caattttgta tattgttttc tcttaaaatc gcaagtttgt
     5761 aatttctcat ctactgaacc gggagtttgt tggaatttga tctcaataat aaataaggta
     5821 tcacggatga tgataaataa gccattgtcg ggtagtaatc ttttagacag atggtctttc
     5881 caattaatat tgtatcccgg tcttttcaaa aatcggtaaa actcatattt tttaaaacaa
     5941 taagccttca attctccatt gaaccaaact tcataacccg cattatcata tgtttttctt
     6001 aagtcgtacc cgggaatttc ttcaaacaac gttcgtaagt ctgtacgttc ttcgaatctg
     6061 agtcctgttt gtgtccttgc accaccgatt ccgccaataa tcatttttct aacctttttt
     6121 ttgctaattc aaaaaaatct tcttccagtt cgcaaccaca aaatttccga ccatgtttta
     6181 aggcggcaac gcctgttgtg ccgctgccca tgaaagggtc aaaaattaaa tctccgatgt
     6241 ttgaagctga gagtatgcag cgttcaagca aggggagtgg tttttgtgtc ggatgtttgc
     6301 cgaatgtttt ttcggctttg tttggaggag gaaatgtcca aacacatttc atctgtttgc
     6361 catcattttg tgctttcatc atttcataat gaaacgtgtg tttggctttt ttatttttct
     6421 ttgcccataa gattgtttct gtcgaatggg taaaaaaacg gcaggacaaa ttagggggag
     6481 gattgggttt ttcccaagta atattgttca aaatatggta tccgatagtt tgcatcaggt
     6541 agccgattaa ataaatatta tggaatgtgc cgcaaaccca aattgtgccg tttggtttta
     6601 ataaggcgta acacagtcgg agccattctt cgtagaattc caaatctgcc gccattcctt
     6661 tggatttatc ccagttgccc ttattgacgg aaaccatttg tccgttttga caactgaagc
     6721 cgtcattgga aagaaagtaa ggcgggtctg agaaaatcat atcaaaacag ccgtttggat
     6781 atttttccaa tatcttccgc atgacgttaa gggaattttc gttgtagagg acaaggtcgt
     6841 ctgaaacgat ttttttcaaa cgactttgat aaatggcgtc ttcttcagcc ggtttgtcaa
     6901 aatcaatcat aaattgccaa taattctccg acttttttac ggctgctgcc ttttgcggca
     6961 atattgcgtt gtacttcaat tgttttcaga atggcaaaag aataaatatc ccttgtgaat
     7021 tcagtatcat gatttgaaat caaaacgcct tgggaatcgg gcgcaatttg ttgggctgac
     7081 tgggcaagac ggatttgatt gtctaaattg aaactttctt tggcataagt ggtaaatgaa
     7141 gcggttgggc ttaaaggcgc gtaaggggga tcgcaataaa ccgtatcggt attgtctgtc
     7201 tgagatagaa tcatttgaaa atctccgcac attaattcaa ctctgtcaga tttctgaatg
     7261 aaaccttcca tttcttgttg gggaaagtac ggcgatttgt agcgtccgaa cggcacattg
     7321 aatacccctt tgctgttgta acggcataaa ccgttaaaag catgacggtt caaataaata
     7381 aatagtgctg agcgttcgtg cagatttttg ctgaagttga attgttcccg caattcatag
     7441 aatcttgaat cgctattgtt ttcggaaata aagaaagagc gggcgtagtc gatgaatccg
     7501 gatttttctt cttttaggat acggaataag ccgattaagt cagcgttaat atcattgagc
     7561 agataatgtt cgaagtccaa tgcaagagac aaagcggcag aaccgcaaaa gggttcaatc
     7621 aggcgttttc gtgtaggagt gggtagatta tgttcgataa aggggacaag tttatgtttg
     7681 ccgcctgccc attttaggaa gggttttgaa gccattagga tgcagagtat aagcgtggtt
     7741 gattggcttg gaattgtgcc aaaacatcgt gaggaataca acggatttat attttgggga
     7801 cgtgtgtaga gtgagtctaa atccaataga acctttccat tccaatatgt tattggactt
     7861 atcaactcca tctgccttaa aaaggccgtc tgaaaccctg tttcccagtt ttcagacggc
     7921 ctttatccgt atggctaaac cttaagagcg gtttagacga cgatattcac caagcgtccg
     7981 ggaacgacga tgattttctt cgccggtttg ccttccatga atttcaccgc gccttcggtg
     8041 gcgagtgcgg cggcttcgag gtcggctttg gatgcgtcag cggcaacagt gattttgccg
     8101 cgcagtttgc cgttgacttg aaccatcact tcgatttcgg atttgaccaa ggcggcttcg
     8161 tcgactgtcg gccagcctgc ttcccacagt ttcgcgccgt tcaattcgct ccacagggtt
     8221 tcgcagatgt gcggcacgat gggccacaac aggcgtacgg cggtttccaa tacttcttgg
     8281 gcgacggcgc gtccttgttc gccgccggtg tcggttttgt cgtattggtt gagcaattcc
     8341 atcacggcgg cgatggcggt gttgaactgc tggcggcggc cgtagtcgtc gctgactttg
     8401 gcagtggtcg cgtgcagttt gtggcgcagg tctttgagtt ctttagacaa accgtcttgg
     8461 ctgcctgcga acgctttgac cgcttcgcct tgcttcaagt attcgtaaac ggtacgccac
     8521 aggcggcgca ggaagcggtg tgcgccttcg acgccgctgt cgctccattc gagggactgt
     8581 tcgggcggtg cggcgaacat cataaacagg cgggcggtgt ccgcgccgta ggcgttaatc
     8641 agttcttgcg gatcgacgcc gttgtttttg gacttggaca ttttttccgt gccgctgatg
     8701 acgacgggca gcccgtcggc tttgaggacg gcggaaatgg ggcggccttt gtcgtcgaac
     8761 gtcagctcga catcggcggg gttgatccaa tctttgccgc ctttgtcgtt ttcgcggtag
     8821 taggtttcgc aaacgaccat gccttgcgtc agcaggcgtt caaacggttc gtcaacattg
     8881 actagacctt cgtcgcgcat cagtttggtg aagaaacgcg cgtacaagag gtgcaaaatc
     8941 gcgtgttcga tgccgccgat gtattggtcg accgcgcccc agtatttcgc ggcggcagga
     9001 tcgaccatgc cgtctgaaaa ttttggcgac atatagcgga agaaatacca gctcgattcc
     9061 atgaaggtgt ccatggtgtc ggtttcgcgt tttgccgcgc cgccgcagca tgggcaggca
     9121 gtttcgtaaa actcgggcat ttttgccagc ggcgaaccca tgccgtcggg tacgacgttt
     9181 tcaggcaaaa cgaccggcaa ttggtcggca gggacgggta cgtcgccgca ttgttcgcaa
     9241 tggacgatgg gaatcgggca gccccagtag cgttggcgcg aaatgcccca gtcgcgcagg
     9301 cggtattggg ttttcggttc gcccgcgcct tggctttgca gcttggcggc aacggcatcg
     9361 aatgccgtct gaaaatccaa gccgtccaag tcgccgctgt tgaccaatac gccgttttct
     9421 ttgtcggcat accattcttg ccatcggttt gcgtcgaatg cgttgtcgcc gacggcaatg
     9481 acttgttttt taggcagatt gtatttggcg gcgaactcaa aatcgcgttc gtcgtgcgcc
     9541 ggaaccgcca tcactgcgcc gtcgccgtag ccccacaata catagttggc aatccacact
     9601 tccagcttgt cgccgttgag cgggttgacg acgtagcgac cggtcggcac gccttttttc
     9661 tccatcgtcg ccatatcggc ttcggcaacc gaaccggctt tgcattcggc aataaatgcc
     9721 tgcaattcgg gtttgtcggc ggctgcggcg gctgccagcg gatgctcggc ggcaacggca
     9781 acataagtcg cacccatcag cgtgtcgggg cgggtggtat aaacttgcag caatttcgcg
     9841 taatcgcctt ccaagccttg tttgctgtcg tctgaaacgg cgaagcgcac ggtcataccg
     9901 cgcgatttgc cgatccagtt gcgctgcatg gttttgactt gttccggcca gtgttccagc
     9961 ttgtccaagt cgttgagcag ctcttcggcg taatccgtga ttttgaagta atacatcggg
    10021 atttcgcgtt tttcgatcaa cgcgcccgaa cgccagccgc gtccgtcgat gacttgctcg
    10081 ttggcaagga cggtttggtc gaccgggtcc cagttcaccg tgccgttttt gcgatagacg
    10141 atgccttttt caaacagctt ggtaaacagc cattgttccc agcggtagta ttcgggtttg
    10201 caggttgcgg tttcgcgcgc ccagtcaatc gcaaaaccca ggcttttgag ctgggttttc
    10261 atgtattcga tgttatcgta cgtccaagcg gcaggggcga cgttgttttt catcgccgcg
    10321 ttttccgccg gcatgccgaa cgcgtcccaa cccataggct gcatgacgtt gaagccgttt
    10381 aaaagtttga agcggctcaa tacatcgccg atggtgtagt tgcgcacatg ccccatgtgc
    10441 agcttgccgc tgggataggg gaacatggag aggcaataat atttgggttt ggaagcgtct
    10501 tcggagacgt tgaaaatacg ggcgtcgtcc cattttttct gcgccgcagg ctcgatggcg
    10561 gcgggttggt aatgttcttg catggtcatt ctgtttttcg cttgaaaacg ttaggaattt
    10621 aaaggaggaa ttataacagg ttgccgatgt cggcgaatcg gcaaattgcc ggcaggatgc
    10681 gtaaattcgc acgcgcatca ttccgtatgc cgtacaaata caccgcgttt actgatacgc
    10741 acgtttttta tgctaatatt acaaaccaaa atcaaatgtt taaaactctc ctgatgcggc
    10801 tcttccgaac aaaaggcaga cgggcatcgg gtaaaagagg attctgcata tgaaaatcaa
    10861 acaaatcgtc aaaccgggct tggcagtatt ggcggcgggc gttctgtctg cctgcgcaac
    10921 caaaagcaac gtcaaagccg acggaacgac cgacaatccg gttttcccga aaccctattc
    10981 cgtaacgctc gacaacaatc gcggtacatt cccgacctat gacgaattgg acttgatgcg
    11041 tcccggtctg accaaagacg acatctacaa aatcctgggt cgtccgcatt acgacgaagg
    11101 tatgtacggc gtgcgcgaat gggattatct gttccacttc cacaccccgg gcgtaggcat
    11161 cgaccctgaa aacacttccg gcgtagaagg cattaccacc tgtcaataca aaattatttt
    11221 cgataaagac aaatttgccc gcagcttcta ctggaacccc gtcttcccga aagatgccgc
    11281 ctgtccgccg cccgcaccca aagccgagcc gcaagtcatc atccgcgaaa tcgtgccggc
    11341 aaaaccgaaa cgtatccgcc aataatccga catgccgttc cgcctgtttt tagggatatt
    11401 atgcggcctg tcaatggttg cccccgtata tgcacagggg caaccggatg cggtcaacgc
    11461 ttatattcaa aagaaaaaag tcatcgtaaa cacatccaaa gcggaactct gttttgccga
    11521 tgacaggcag tgccaccccg tcctcatcgg tgttgccacg cccaagggga cgttcgggct
    11581 gacgctgaac agtaccgaca agcctggata cggcggcgaa gtcatcggtt tcaagcagga
    11641 gggtgatttt cttttcgccc tgcaccgcgt ttggaatcag ataccgtcgg aaaggcggaa
    11701 cgaacgcatc gcctccccgt ccgtgtccga caggattatg accaacggct gcatcaacgt
    11761 cagcgatgcg gtgtacgaaa aactgcgtca ttattttgtg ttggaagtga tttgaaacag
    11821 acggataccg cacgcgccgg tatctgtttt cacattgccc cgatgcctga aacagactgt
    11881 ccgccacgtc atgccgtctg aaaccggcgc agatgccgcc aagccttcag acggcattgc
    11941 ctgcccgctc cgaccgaaca acaaccatct ttgggagaac cttatgcccg aacaaaaccg
    12001 catcctctgc cgcgaactaa gcctgctggc attcaaccgc cgcgtgttgg cacaggcgga
    12061 agacaaaaac gtcccccttt tggaacgcct gcgcttcctg tgcatcgtgt cgtccaatct
    12121 tgacgagttt ttcgaagtcc gcatggcgtg gctcaagcgt gaaaacaagc tgcacccccg
    12181 gcgcaggctg gacaacggca aaatgccgtc tgaaaccatc gccgacgtta ccgaagcggc
    12241 gcgctccctg atacggcatc agtacgacct gttcaacaac gtccttcagc ccgagttggc
    12301 gcgggaaagc atccattttt accgccgccg caactggacg ggcacgcaga aaaaatggat
    12361 cgaagactat ttcgaccgcg aactgctgcc gatcctgacc cccatcggac tcgacccgtc
    12421 ccaccccttc ccgcgaccgc tgaacaaatc gctcaacttc gccgtcgaac tcgacggcac
    12481 ggacgcattc ggcaggcctt cggggatggc gattgtgcag gcaccgcgca tcctgccgcg
    12541 cgtcgttccc ctgccgtccg aactgtgtgg cggcggacac ggcttcgtct tcctgtcgtc
    12601 catcctccac gcccacgtcg gcaaactctt cccgggcatg aacgtcaaag gctgccacca
    12661 gttccgcctg acgcgcgaca gcgacttgac cgttgacgaa gaagacctgc aaaacctccg
    12721 cgccgccatc caaaacgaac tgcacgaccg cgaatacggc gacggcgtgc ggctcgaagt
    12781 cgccgacacc tgtcccgcct acatccgcga ctttctgctc gcgcaattca aactgaccgc
    12841 cgccgaactc tatcaggtca aaggcccggt caacctcgtg cgcctcaacg ccgtccccga
    12901 cctagtcaac cgccccgatt tgaaatttcc cacacacacg ccgggcagac tgaaagcctt
    12961 gggcaaaacc gcgtccatat tcgatttggt gcgccaatcg cccatcctgc tgcaccaccc
    13021 ctaccaatcg ttcgaccccg ttgtcgaaat gatgcgcgaa gctgccgccg accccgacgt
    13081 gcttgccgtc aaaatgacga tttaccgcac cggcacgcgt tccgaactcg tccgcgccct
    13141 gatgaaggcg gcactcgccg gcaaacaagt aaccgtcgtc gtcgaactga tggcgcgttt
    13201 tgacgaagcc aacaacgtca actgggcgaa gcagctcgaa gaggcgggcg cgcacgtcgt
    13261 gtacggcgtg ttcggctaca aagtccacgc caagatggca ctggtcatcc gccgcgaaga
    13321 cggcgtactc aagcgttacg cccacctcgg cacgggtaac taccaccaag gcacatcgcg
    13381 catctacacc gacttcggca tcattaccgc cgacgaacaa atcaccgccg atgtgaacac
    13441 attgtttatg gaaatcacag gcttgggcaa acccgggcgg ctgaacaaac tctaccaaag
    13501 cccgtttacc ctgcacaaaa tggttatcga ccgcatcgca cgcgaaaccg aacacgcaaa
    13561 agccggcaaa ccggcgcgga ttaccgccaa gatgaattcg ctcatcgaac cgaccgtcat
    13621 cgaagccctg tatcgggcaa gcgcggcagg cgtacaaatc gatttgattg tgcgcggtat
    13681 gtgcaccttg cgcccgggtg taaaaggctt gtccgaaaac atccgcgtcc gctccatcat
    13741 cggcaggcag ctcgaacacg cgcgcgtgta ttgcttccac aacaacggcg cggacgatac
    13801 ctttatctcc agcgcggatt ggatggggcg caacttcttc cgccgcatcg aaaccgccgc
    13861 gccgattgcc gcgcccgaac tcaaaaaacg cgtcatccgc gaaggtttgg aaatggcact
    13921 ggcagacaat acgcacgcgt ggctgatgca gcccgacggc ggctatatcc gcgccgcgcc
    13981 tgccgagggc gaaagcgaag ccgacctgca aaacgatttg tggacactgc tcggaggctg
    14041 acccgcaccg ccccaatcaa aaaccatgcc gtctgaaacc tttccgtttc agacggcatg
    14101 gttttacagc aatctaaaca gggcggactg gaatcaaaaa cacaccttcg ccattcccgc
    14161 acaagcactt cccctatacg ctcccaaccc caatccgccg cattccagac ggcattgcct
    14221 ttccgacagc cgaacaaaac cccgaatccg tctgcacggt tcggggcata tctccaatac
    14281 gggcatcgtg ctccggaaaa ccgtcaaatc cgcatcggca tcacaatata tttgaaattc
    14341 ggattgttcg gcacggtaaa cagcgtcgag cggttggcat cgccgaaggc aagctgcata
    14401 tcgtcggaat ggatgttgcg caacacgtcc atcagatagc cgatattgaa accgacttcg
    14461 agttcgccgc cctgataggc aatctcgatt tcttcgcgcg cttcttcctg ctcgttgttg
    14521 ctgcacacga cgctcaacag gccggattgc aggaacaggc gcgcgccacg gaatttttcg
    14581 ttggcaagaa tggacacacg ttccagcgcg cccaaaagtt cggcacggga caaaacgaaa
    14641 atcttgtcgt tgtccaaagg gatgacgcgg ttgaaatcgg ggaatttgcc gtcgataacc
    14701 ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct gcttgtccag cagctcgatt
    14761 tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc
    14821 aaaatcactt cggcgcgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg
    14881 tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg
    14941 agataatagc ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg
    15001 aaacgctcct gctccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga
    15061 aaatcggcgg caggcagggt ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga
    15121 cggctgtcgt cccaatccag cgacaccagc gcaccggcag gcagcgcgcg caaaatgtcc
    15181 tgaaatttct tggcattggt ggtgatgcgg aagtcgtccg cgccgccctc gggacccgca
    15241 gtgtcgattt ggatttccaa atcggttgcc aagagtttgg tctgaccgcc ttttccctca
    15301 atcaggacgt tggacaggat gggcagggtg tggcggcgtt cgacgatgcc ggtaacggct
    15361 tgcagcggct tgagcaggct gtcgcgttcg gcttgtaaaa tcaacatgtt cgctccttta
    15421 aatcggttgg attttttacc cttcggacgg catgggcaat atcatgccgt ctgaaaacgt
    15481 tttccatcag ttttgaatca gaatcagcag cttttcataa tcctgagcca attccggatc
    15541 ttcttcgcgc agtttcgcca ctgccctgat gccgtgcata acggtcgtat ggtcgcgccc
    15601 accgaacgaa tcgccgatag acggcaggct caaagtggtc agttctttgg tcaggctcat
    15661 cgccacctgg cgcggacggg caatgttgcg cgtgcgtttt ttgccgagta cgtcgctgat
    15721 tttgatgcgg taatatttgg caacggcatc aataatgata tcggcggtaa tgaccttatg
    15781 cttctcggca ataatgtcct gcaaagcggt acgcgccaaa tcgatgtcga tgacgggacg
    15841 gttcataaag cggctgctcg ctccgacacg gttaaacgcg ccttcaagct cgcgcacgtt
    15901 ggaacggatc agattggcaa tgaacagcgc ggcttcgtct tcaatactga tgcccgccgc
    15961 ttccgccttt ttctgcaaaa tggcgatgcg catttccaat tcgggcggct cgagttccaa
    16021 agtcagtccc cataaaaaac gggatttgag gcggtcgtcc atgccttcga ttttggcggg
    16081 taaaacatcg caagtgagga tcagctgttt tttctcgttg tggaaatggt tgtacagata
    16141 gaaaaactct tccatcgtac ggtcttttcc tttaataaac tgaatatcgt cgataatcag
    16201 caggtcgtat tgcttgtatt gctgcttgaa tacgtcatag gtattgttgc gaaccgcctt
    16261 cataaagctg cggatatagt cgtccgaatg catatagcgc actttggcat cgggacggtt
    16321 tttcaaaagc tcgttgccga ccgcctgtac caggtgggtt ttgcccaaac ccgtgctgcc
    16381 gtacaggaaa aacgggttgt agctctgccc cgggctttcc gcaatcgcct gcgccgcagc
    16441 cgccgcaagg cggttgccct tgccttcgac caatgtgtcg aacgtatagt ccggcgacag
    16501 attggtctgc tcgtaacgtg cctcttccgc atcgcgctgc gcatccgtcc gcgctttcgc
    16561 aagcacctcc gattccggac gggaagcagg ttcggcagta tggcgcggct catgcgggag
    16621 gtttttcata cgttccgcca gaatatccgc cgccgttttc gacgcagcgg gtttgacagg
    16681 ctcttcagac ggcagctcgt ccaacagaac ctcctgcaca ggcatctcct ccgaccccgc
    16741 gtgcaaggac ggctcggcag gttcgacagc accttcaacc gccgccatct cataacgcac
    16801 gccttctcct ggtttgaata cgaaggcgga acggccagca gccaactctt ccctcaccgc
    16861 ttctattttc ccggcaaact ggctcttaag catattgcag gcaaactggt tcttgccgta
    16921 caccacccat acgccaccct cctcaccgac ggtaaggggc gcaatccatt gcgcaaactg
    16981 cccttgaggc aacatatcgt gaagacggcg gaggcacagc ggccaaaact ctgctaatgt
    17041 catggataag ctcgaatcgg taaaaatgaa atcgaaaaca aagaaaatat aatattttca
    17101 aaaagaaaac aaatctgttg aacacacatc ggttcaaaat gcagcgaacc gaattatacc
    17161 gactcgccga tattttatcc acaacccgtg caaaatttat ccacagaaag gcggcagaaa
    17221 tccgcaggca atcgggcaat cctcctgcaa agtttctata ttgattgaca aaagcggcaa
    17281 attggagtgt aattcacggt ttaattatct acccattcta tttttaggaa acatcatgaa
    17341 acgcacttat caaccttccg ttaccaaacg caaacgcacc cacggcttcc tggtgcgctc
    17401 caaaacacgc ggcggccgcg cagtattggc tgcacgccgc gccaaaggcc gcaaacgcct
    17461 ggcggtataa ttttggacta ccgcttcgga aggcagtacc gcttgttgaa aacggatgat
    17521 ttttcatccg tttttgcatt cagaaaccgc cgcagccgcg acctgctgca agtttcgcgt
    17581 tcaaacggca acgggctgga ccatccccgc atcggtctgg tggtcggcaa aaaaaccgcc
    17641 aaacgcgcca acgaacgaaa ttatatgaag cgcgtcatcc gcgactggtt cagattgaac
    17701 aaaaaccggc tgccgccgca ggatttcgtc gtgcgcgtcc accgtaaatt cgacaggtct
    17761 accgcaaaac aggcaagggc ggaactggca caactcatgt tcggcaaccc cgcaaccgga
    17821 tgcaggaaac aggcatgatc agaacggtac tctgcaggca aggttcagac agcaacgggt
    17881 ttcccataca aggaacatcc cgatgaactt cctattgtcc aaactcctgc tgggactgat
    17941 acggttctac caatattgca tcagcccgct gattccgccg cgctgccgct atacgccgac
    18001 ctgttcgcaa tacgccgtcg aagcagtcaa aaaatacggc gcattcaaag gcggccggct
    18061 cgccatcaag cgcattgcac gctgccaccc cctcggcgga cacggacacg accccgttcc
    18121 ctgacccgac gcaatattca aattgcacgc tttcctttta tttcccatcg gtttctatat
    18181 aatgccgtct gaagcttcgg gcaggcggca cgaccgtcgg gtatgaaacc cgcccttatt
    18241 ccccgtctat cggaacacgc aacctgcggc atttccgacc attcaggaaa ctcttatgga
    18301 ttttaaaaga ctcacggcgt ttttcgccat cgcgctggtg attatgatcg gctgggaaaa
    18361 gatgttcccc actccgaagc ccgtccccgc gccccaacag gcagcacaac aacaggccgt
    18421 aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac
    18481 ggttcaagcc gtcattgatg aaaaaagcgg cgacctgcgc cggctgaccc tgctcaaata
    18541 caaagcaacc ggcgacgaaa ataaaccgtt catcctgttt ggcgacggca aagaatacac
    18601 ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctaa aaggcatcgg
    18661 ctttagcgca ccgaaaaaac agtacagctt ggaaggcgac aaagttgaag tccgcctgag
    18721 cgcgcctgaa acacgcggtc tgaaaatcga caaagtttat actttcacca aaggcagcta
    18781 tctggtcaac gtccgcttcg acatcgccaa cggcagcggt caaaccgcca acctgagcgc
    18841 ggactaccgc atcgtccgcg accacagcga acccgagggt caaggttact ttacccactc
    18901 ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca gcttttccga
    18961 cttggacgac gatgccaaat ccggcaaatc cgaggccgaa tacatccgca aaaccccgac
    19021 cggctggctc ggcatgattg aacaccactt catgtccacc tggattctcc aacctaaagg
    19081 cagacaaagc gtttgcgccg caggcgagtg caacatcgac atcaaacgcc gcaacgacaa
    19141 gctgtacagc accagcgtca gcgtgccttt agccgccatc caaaacggcg cgaaagccga
    19201 agcctccatc aacctctacg ccggcccgca gaccacatcc gtcatcgcaa acatcgccga
    19261 caacctgcaa ctggccaaag actacggcaa agtacactgg ttcgcctccc cgctcttctg
    19321 gctcctgaac caactgcaca acatcatcgg caactggggc tgggcgatta tcgttttaac
    19381 catcatcgtc aaagccgtac tgtatccatt gaccaacgcc tcttaccgct ctatggcgaa
    19441 aatgcgtgcc gccgcaccca aactgcaagc catcaaagag aaatacggcg acgaccgtat
    19501 ggcgcaacaa caggcgatga tgcagcttta cacagacgag aaaatcaacc cgctgggcgg
    19561 ctgcctgcct atgctgttgc aaatccccgt cttcatcgga ttgtattggg cattgttcgc
    19621 ctccgtagaa ttgcgccagg caccttggct gggttggatt accgacctca gccgcgccga
    19681 cccctactac atcctgccca tcattatggc ggcaacgatg ttcgcccaaa cttatctgaa
    19741 cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc
    19801 cgtcatgttc ttcttcttcc ctgccggtct ggtattgtac tgggtagtca acaacctcct
    19861 gaccatcgcc cagcaatggc acatcaaccg cagcatcgaa aaacaacgcg cccaaggcga
    19921 agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc agacggcatt
    19981 tttattgccc accccctatc ggggcggaaa tcttcaaccc gcatacatca caaaaatcgt
    20041 cgggcgtttt ttcagattgg gcatttcttt tctttttcgc cactgcacga ttgtttgact
    20101 gatgatttcc tgtgtcggca aggtcaaatc cgtagccgtg cataaacgcg tttcaggatg
    20161 caggttttcc accgcatcgg caagcagcgc atcattgcgg taaggcgttt caataaaaat
    20221 ctgcgtctcg ccgcactggc gcgaacgctg ttccaaagcc cgaaaagcct gaatccgctc
    20281 gtttttttca gacggcagat agcctttaaa cgcaaaactc tgcccgttcg cacccgaagc
    20341 catcaaagcc agcagcaggc tggaaggccc gaccagcgga cgcacttcaa aaccgtgttt
    20401 atgcgccaat gccaccaaat tcgcacccgg atcggccaca gccgggcaac ccgcctcact
    20461 gacaatgccc atactgcgcc cttcttgcaa aggtttcagc aattccggca aagtcttcaa
    20521 atccgtatgt tcattcaacg tttgcagatt cagctcgcgg ataggcgtag tcacgcccaa
    20581 atgtttcaaa tgcgcacgcg ccgttttttc cgcctccacg acaaaatccg tcagcccgac
    20641 aatcgcctgt tgttcatgcg gcaacaggca cggcgtgtca ggcgtaccca aaggcgtagg
    20701 aatcaaatac aaaacaggag acatcattcc ctcactcatc ggttaaaaat gccgtctgag
    20761 cctttcagac ggcataaacg ggcagttaca gaacctccac gccctcattt ttcaagaaat
    20821 cgaccagacg gaaaaccggc aaaccgatta aagcattcgg atcggtactc tcaatccttt
    20881 caatcagcaa tgcacccaaa tcctcactct tcagcgcaca cgaacaataa accgcatcag
    20941 gctcgcgctc caaatagcgg aggatatgca actcgtccaa ctgcctcatc acgaccaccg
    21001 tcttatcgat atgccgccgc atcctgcccg taaccgtatt caacagcacg atcgcgctgt
    21061 aaaactcaat ctccctgccg ctcaagtgca tcagcatctt ttgcgcattg gcaaggttca
    21121 tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg
    21181 cctctgggaa acgcccggtc aacgaccgcg ccttaccctc ggcaaggcgc aatgccgtct
    21241 gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggacgcc gcctgaaagg
    21301 caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc aaaatcaaag
    21361 gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag gggcagtaaa
    21421 atccagccat gcgccgaaac acggataccc gccttcggcg taccgcaaca tttttcttaa
    21481 aaatattgac gttagaacat ctaaattata tcatatcccg tttatgtcag accctaattt
    21541 gattgacttg gaaatttttg ccgccgaagg gcagaacctg caaggcagtt ttctgctgga
    21601 agaattggat gaacgcgtca gttcgcacga ttatcccgcc gacaggcaga ccaaaatatc
    21661 gtttacactg accggcggtc gcgaccggct gcaacgcctg ttcctcgacc tgaacgtcaa
    21721 agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca tgcttgatga
    21781 aagcagccgt atcgtcctgt tttccaacga agagtccttg gacgaatcca tgcttgccga
    21841 cgaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcacat tggtagaaga
    21901 ccaaatcctg atgtccctgc ccttttcgcc gcgacacgaa gactgcggcg acaatgggac
    21961 actggaagaa gtcaatcggg acaaacccaa cccctttgct gttttggcag gtttgaaaag
    22021 caattgatta ggacacagtt tatttatcta ggagcttgaa atggccgttc aacaaaacaa
    22081 aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcgctgaccg cgcctgcact
    22141 gtctgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatctccc ccaacggtat
    22201 gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg actgactgaa
    22261 aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca ccatccgtcc
    22321 aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataatac cccgtgaaaa
    22381 tattccaaaa gccccaacca ccaaggaaat tccgatgaaa cagaaaatct ggtacaccta
    22441 cgatgacatc caccgcgtca tcaaagcatt ggcagaaaaa atccggaacg ccgacatcaa
    22501 atacgatgcc atgattgcca tcggcggcgg cggctttatt ccggcacgta tgctgcgctg
    22561 ttttctggaa attccgattt atgccgtaac caccgcctat tacgacagcg acaacgaagg
    22621 acaggttacc gaagaagtca aaaaagtcca atggctagac cccgtccccg aagccttacg
    22681 gggtaaaaac gtccttgttg tcgacgaagt ggacgacagc cgcgtaacca tggaattttg
    22741 cctgacagag ctgctcaaag aagacttcgg caccatcggc gtcgccgtac tgcacgaaaa
    22801 aatcaaagcc aaagcaggta aaatccccga aggcattccc tatttcagcg gcatcaccgt
    22861 agaagactgg tggatcaact atccgtggga cgcactcgac atcgacgaac acaaccgcct
    22921 tgccgaggcc ggccgaggct gaccctttca gacggcatat tttccgaacc gatgccgtct
    22981 gaagcccgca cgacccctgc cgcagaccga aaacctaccg gagaacccta tgattacact
    23041 tgccgtagat gccatgggcg gcgaccaagg acttgccgtt accgtacccg gcgcaaccgc
    23101 attcctccaa gcacaccccg atgtccgcct gattatgacc ggcgacgaaa cgcaactgcg
    23161 ccaagccctg accgcggcag gcgcaccgat ggaacgcatc gacatctgcc ataccaccca
    23221 agttgtcggc atggatgaat cccctcagtc cgccctaaag aacaaaaaag actcctccat
    23281 gcgcgttgcc atcaaccaag ttaaagaagg caaagcccaa gccgccgtat ccgcaggcaa
    23341 cacgggcgca ctcatggcaa ccgcacgttt cgtcctcaag accatacccg gtatcgaacg
    23401 ccccgccatc gccaaattcc tcccgtccga caccgaccat gttaccctcg ccctcgacct
    23461 tggcgcgaac gtcgactgca cgcccgaaca gctcgcccaa tttgccgtta tcggcagcga
    23521 actcgtccac gcactccatc ctcaaaaagg acagccgcgc gtcgggctgg tcaacgtcgg
    23581 cacggaagac atcaaaggta cggacaccgt caaacaaacc tacaaactgc tgcaaaacag
    23641 caaactcaac tttatcggca acatcgaaag caacggcatc ctgtacggcg aagcagatgt
    23701 cgtcgtcgcc gacggctttg tcggcaacgt catgctcaaa accatcgaag gcgcggtcaa
    23761 attcatgagc ggagccatcc gccgcgaatt ccaaagcaac ctgttcaaca aacttgccgc
    23821 cgttgccgcc ctacccgccc tcaaagggct gaaaaacaaa ctcgatccgc gcaaattcaa
    23881 cggggccatc ctgctcgggc tgcgcggcat cgtcatcaaa agccacggcg gcacagacga
    23941 aaccggtttc cgctacgcac tcgaagaagc ctaccacgaa gccaagtccg ccggcctttc
    24001 caaaatcgaa caaggcgttg ccgaacaact cgccgcactc gaaactgcca aagccgtcca
    24061 aaacgaaaat gccgacggtc tgtaacacac acgatgccgt ctgaacgccc ccgccccttt
    24121 cagacggcat ccgcccgcac caaacctgcg ggcgcggacg gcgatgcgcc tgtccggcac
    24181 ttcccaaata cttccctgta aaataaggag tatttgaaaa atgaagacat tagaaaaacg
    24241 gatgaaagct ctagacaaac agatgatgaa gttcggaaaa tcccttgaag gcaggcttga
    24301 tgcccgtctg attgaatccg cattggatta tattcattat tcggaacgtt ttttggcttt
    24361 tgaaatcctg tgtacttata tcgaagattt cgatgtccgg ctgacggaac aagaatcccg
    24421 ggaaatttct tttatcaaca aggaatttga gatagaaagc acgtccgatt aaccaataaa
    24481 gccaatgggt tgataaacat gaaaacatcg acggtcgttt ttggcggatt ttttatggca
    24541 gacaacggag agcgaatcca aatccccgtt ttggaaaatc ctgacattag ggaaatcaat
    24601 cacttttttt ccgtatcaaa ttttgagaaa aaaaccggcg tccttgtttt cagaatcatc
    24661 cccgagccgg aatttggcaa taccgaatta actgtctatt ttgaaaaagg atattatttg
    24721 ccaatgattc aaacaatttt agaaggcggg gatattgaag tcaaaaacct taaaacggaa
    24781 aattatagtg gaaatacaat ggaaatttgg ggagattcct accctattga gcatatctct
    24841 aaaaatattt ctacaattca aaatataatt tctgaattta tgatacaaaa acaaacaccg
    24901 gcaccaataa tttgatggaa aaccaacaat caaaatgccg tctgaagcat gtttggtttt
    24961 cagacggcat ttccttcgct taaaacagcg tatcggcaac cccgccctgc ctgtccacgg
    25021 caatctgcat ctgaaaacca tctgtatccc aaaccacacc cccatccctg tttccgtcat
    25081 ttgcaccctg cccgtattgg gcaatcatcg gtttttcgct tacaatagcc gaatctgaac
    25141 caactatcca aaaaggccgt tcccatgcag tatgccaaaa tttccggcac gggcagctat
    25201 cttcccgcca accgcgtcag caatgacgac cttgcccaaa aagtggatac ctcggacgag
    25261 tggattaccg cgcgtacggg catcaagttc cgccatattg cagccgaaaa tgagaaaacc
    25321 agcgatcttg ccgccgaagc agcccgccgc gcactggatg cagccggatt agacagcggc
    25381 gaaatcgatt tgattatcgt ggcgacggca acgccggata tgcagtttcc ttctaccgcc
    25441 accatcgtgc agcaaaaatt gggcatcacc aacggctgtc ccgcgtttga cgtacaggca
    25501 gtgtgcgcag gctttatgta cgcgctgacc actgcaaacg cctacatcaa aagcggcatg
    25561 gcaaaaaacg cgctggtcat cggcgcggaa accttcagtc gcattgtaga ctggaacgac
    25621 cgcacaacct gcgtattgtt cggcgacggc gcgggcgcgg tggttttgag cgcggcggac
    25681 aaaccgggca tcatccacag caaactcaaa gccgacggca attatctgaa actcttaaac
    25741 gtccccgggc aaatcgcctg cggcaaagtt tccggttcgc cgtacatttc gatggacggt
    25801 cccggcgtgt tcaagtttgc cgtcaaaatg ctgtccaaaa tcgccgatga cgttatcgaa
    25861 gaagccggtt ataccgccgc tcaaatcgac tggatcgtgc cgcatcaggc aaaccgccgc
    25921 attatcgaat cgaccgcgaa acatttaggt ttgagtatgg acaaagtcgt cctgaccgtc
    25981 caagaccacg gcaacacatc cgccgcatcg attccgctgg ctttggatac gggcatccgc
    26041 agcggacaaa tcaaacgcgg tcaaaacctg ctgctcgaag gcatcggcgg cggtttcgcg
    26101 tggggcgcgg tattgctgca atattgaata ccatgccgtc tgaagcaggt tttcagacgg
    26161 tatttcccat atcatgaagc ggcaggcttt cttcaaacca attgcgtgtg cggcatttct
    26221 gtctgccatt tcgctgcgcc tccccgtatt gggcgcgtgt tacgcaatat tgtccctcta
    26281 tgcgtttgca ctttacggca tcgacaaacg gcgtgccgtg cggggaaaac gccgcattcc
    26341 cgaacaccgc ctgctgctgc ctgccttgtt cggcggttgg gcgggcgcat acttgggcag
    26401 caggatattc aggcataaaa cggcgaaaaa gcgttttgtt gtgctgttcc gtctgactgt
    26461 ttcaggtaat gtcctggcga ccctcatcct gattgattat ttcgttccgc ccgaactttt
    26521 tgtcaaactc gggcaacatc tctgacaatc caaccaaaca aaggaatacc atgtcttttg
    26581 cattcttctt tcccggacaa ggttctcaaa gcctcggcat gatgaacggc tttacagggc
    26641 aggccgtcgt caaaaacaca tttgacgaag catccgccat attgggtcaa gacttgtggg
    26701 cgatgattaa cggtagcgat gccgaaatca tcggtcaaac cgtcaatacc caacccatca
    26761 tgctcgccgc cggcgttgcc gtttaccgcg cctatttgga agcgggcggc aaaacgcctg
    26821 ccgccgttgc cggacacagc ctcggcgaat ataccgcact cgttgccgcc ggcgcattgg
    26881 attttgccga cgcggtcaaa ctcgtgcgcc tgcgcgccga actgatgcag tctgccgtac
    26941 cgcaaggcgt gggcgcaatg gcggcgattc tcggcttgga agatgagcag gtaaaacaaa
    27001 tttgcgccga agccgcccaa ggcgaagtgg tcgaagccgt caacttcaac tcacccggac
    27061 aaatcgtgat tgcaggcaat gccgccgccg tcggacgcgc catggctgcc gccaaagaag
    27121 ccggtgccaa acgcgccctg ccgctgcccg tgtccgtacc ttcccattgc agcctgatga
    27181 aacccgccgc cgacaaactc gccgaagccc tcaaaaccgt cgaaatcaag cagccgcaaa
    27241 tccgcgtcat ccacaacgcc gacgttgccg cctacgatga tgccgacaaa atcaaagacg
    27301 cgctcgtccg ccagctttac agccccgtac gctggacaga aaccgtcaac gcccttgttt
    27361 cagacggcat tgccgaatcc gccgaatgcg gcccgggcaa agtgttggcg ggcttggcaa
    27421 aacgcatcaa caaggccgcc gcgtgcagcg cactgaccga tgccggacag gttgccgcct
    27481 ttatcgaagc gcactgactt cgttccgcaa aaagcagcct gccctcttca ggctgctttt
    27541 cacgtccgaa cggcaaacag ccgcatattt gcgctataat tgatgacgac caaaccaccg
    27601 acagcggctg ccgttgcagt tcccgcccta ccgatatgat agaaaaactg actttcggac
    27661 tgtttaaaaa agaagacgcg cgcagcttta tgcgcctgat ggcgtacgtc cgcccctaca
    27721 aaatccgcat cgttgccgcc ctgattgcca ttttcggcgt tgccgccacc gaaagctacc
    27781 ttgccgcctt catcgccccc ctgattaacc acggcttttc cgcacctgcc gcgccgcccg
    27841 agctgtctgc cgccgccggc atcatttcca ccctgcaaaa ctggcgcgaa cagtttacct
    27901 atatggtttg ggggacggaa aacaaaatct ggaccgtccc cctcttcctc atcatcctcg
    27961 tcgtcatccg tggcatctgc cgctttacca gcacctatct gatgacttgg gtctccgtga
    28021 tgaccatcag caaaatccgc aaagatatgt ttgccaaaat gctgaccctt tcctcccgct
    28081 accatcagga aacgccgtcc ggcaccgtac tgatgaatat gctcaacctg accgaacagt
    28141 cggtcagtaa cgccagcgac atcttcaccg tcctcacgcg cgacacgatg atcgttaccg
    28201 gcctgaccat cgtcctgctt tacctcaact ggcagctcag cctcatcgtt gtcctgatgt
    28261 tccccctgct ctccctgctc tcgcgctact accgcgaccg tctgaagcac gtcatttccg
    28321 actcgcaaaa aagcataggc acgatgaaca acgtgattgc cgaaacccat cagggacacc
    28381 gcgtcgtcaa gctgttcaac gggcaggcgc aggcggcaaa ccggtttgac gcggtcaacc
    28441 gcaccatcgt ccgcctcagc aaaaaaatca cgcaggcaac ggcggcacat tccccgttca
    28501 gcgaactgat tgcctcgatt gccctcgccg ttgtcatctt catcgccctg tggcaaagcc
    28561 aaaacggcta caccaccatc ggcgaattta tggcattcat cgtcgcgatg ctgcaaatgt
    28621 acgcacccat caaaagcctt gccaacatca gcatccctat gcagacgatg ttcctcgccg
    28681 ccgacggcgt atgtgcattt ctcgacaccc cgcccgaaca ggacaaaggc acgctcgcac
    28741 cgcagcgtgt cgaagggcgc atcagcttcc gcaacgtcga tgtcgaatac cgttcggacg
    28801 gcatcaaagc cctcgacggc ttcaacctcg acatcagaca aggcgaacgc gtcgccctgg
    28861 tcggacgttc cggcagcggc aaatccaccg tcgtcaacct gctgccccgc tttgtcgaac
    28921 cgtctgccgg caacatctgc atagacggta tcgacatcgc cgacatcaaa ctcgactgcc
    28981 tgcgcgccca atttgccctc gtctcccaag acgtattcct gtttgacgac accctgtttg
    29041 aaaacgtccg atatagccgt cccgacgcgg gcgaagccga agtcctatcc gccctccaag
    29101 ccgctaacct gcaaagcctg atcgacgcat caccgctcgg actgcaccag cccatcggat
    29161 cgaacggcag caacttatcc ggcggacagc ggcaacgcgt tgccattgcc cgcgccattt
    29221 tgaaagacgc gccgatatta ttattggacg aagccaccag cgcattggac aacgaatccg
    29281 aacgcctcgt ccaacaggcg ctcgaacgcc tgatggaaaa ccgcaccggc atcatcgtcg
    29341 cccaccgcct gaccaccatc gaaggggccg accgcatcat cgtaatggac gacggcaaaa
    29401 tcatcgaaca aggcacacac gaacaactga tgtcccaaaa cggctactac acgatgttac
    29461 gcaatatctc aaacaaagat gccgccgtcc ggacggcata aacaaaatgc cgtctgaaat
    29521 ggtacaatcg ccccgaccct ttcagacggc atcatatccg ccgacccatc cgattatctt
    29581 caatcactgt aaaacccatt atgacccaag acaaaatcct catccttgac ttcggttcgc
    29641 aagttaccca gctcatcgcc cgccgcgtgc gcgaagccca cgtttactgc gagctgcatt
    29701 ccttcgatat gcctttggac gaaatcaaag ccttcaaccc caaaggcatc atcctttccg
    29761 gcggccctaa ttccgtttac gaatccgact atcaagccga taccggtatc tttgacttgg
    29821 gcattccgat tttgggcatt tgctacggca tgcaatttat ggcgcaccac ttgggtggtg
    29881 aagtgcaacc cggcaaccag cgcgaattcg gttacgcgca agtcaaaacc atcgacagcg
    29941 agctgacacg cggcattcaa gacgatacgc caaacacgct cgacgtatgg atgagccacg
    30001 gcgataaagt ttccaaactg cctaccggtt tcaccgtcat cggcgacacg ccgtcctgcc
    30061 cgattgccat gatggaaaac gccgaaaaac aattctacgg catccaattc caccccgaag
    30121 taacccacac caaacaaggc cgcgccctgt tgaaccgctt tgtcttggat atttgcggcg
    30181 cgcaacctag ctggacgatg ccgaactaca tcgaagaagc cgttgccaaa atccgcgaac
    30241 aggtcggcag cgacgaagtg attttgggtt tgtccggcgg cgtggactct tccgtagccg
    30301 ccgcgctgat tcaccgcgcc atcggagacc aactgacctg cgtgttcgtc gatcacggcc
    30361 tcttgcgctt gaacgaaggc aaaatggtga tggatatgtt cgcccgcaat ttgggtgtga
    30421 aagtgattca cgtcgatgcc gaagggcagt ttatggagaa actcgccggc gttaccgatc
    30481 ccgagaaaaa acgcaaaatc atcggtgcgg aatttatcga agtatttgat gctgaagaaa
    30541 aaaaactcac caacgccaaa tggctggcgc aaggcacgat ttaccctgac gtaatcgaat
    30601 ccgcaggcgc gaaaaccaaa aaagcccacg ccatcaaatc ccaccacaat gtcggcggcc
    30661 tgcctgaaaa catgaagctc aagctgcttg agccgttgcg cgatttgttc aaagacgaag
    30721 tgcgcgaact gggcgtggct ttgggtctgc cgcgcgaaat ggtgtaccgc catcccttcc
    30781 ccggtcccgg tttgggcgtg cgcatcttgg gcgaagtgaa aaaagaatac gccgacttat
    30841 tgcgtcaagc agacgatatt tttattcaag aattacgcaa caccaccgac gaaaacggca
    30901 cgtcttggta cgacctgacc agccaagcct tcgccgtgtt cctgcctgtg aaatccgtcg
    30961 gcgtaatggg cgacggacgc acttacgact acgtcgtcgc actgcgcgca gtcatcacca
    31021 gcgactttat gaccgcacat tgggctgagt tgccttactc actgctcgga cgcgtgtcca
    31081 accgcatcat caacgaagtc aaaggcatca accgcgtggt gtacgatgtc agcggcaaac
    31141 cgcctgccac catcgagtgg gaataaccag caaacagggc tgcaccgtcc ggcgcagtct
    31201 ttcgattatc ggaaaaaagg aaaaaatatg agcacacaag atttaagcgg caaaatcgct
    31261 ttggtaacag gcgcctcgcg cggtatcggt gcagcaattg ccgacacact ggcggcagcc
    31321 ggtgccaaag tcatcggtac ggcgaccagt gagagcggtg cggcggcgat tagcgagcgg
    31381 ttggcgcaat ggggcggcga aggccgcgta ttaaattccg ccgaacccga aaccgtcgaa
    31441 aacctgattg ccgacatcga aaaaacgttc ggcaaactcg atattctggt caacaacgcc
    31501 ggcatcaccc gcgacaacct cctgatgcgt atgaaagaag aagagtggga cgacatcatg
    31561 caggtcaacc tcaaatccgt ctttcgcgcc tccaaagccg tcttgcgcgg catgatgaag
    31621 cagcgcgccg gccgtatcat caacatcaca tccgtcgtcg gcgtgatggg caatgccggt
    31681 caaaccaact atgccgcggc aaaagcaggc ttaatcggtt tctccaaatc catggcgcgc
    31741 gaagtcggca gccggggcat taccgtcaac tgcgtcgccc ccggctttat cgataccgac
    31801 atgacccgcg ccctgccgga agaaacccgc caaaccttta ccgcccaaac cgccttgggc
    31861 agattcggcg acgcgcaaga cattgccgat gcagtcttgt tcttggcttc cgatcaggca
    31921 aaatacatta ccggtcaaac gctgcacgtc aacggcggca tgctgatgcc ctgacgggca
    31981 gccgtaccga caatgccgtc tgaagccctt tcagacggca tttgcattct caggcaaaat
    32041 gaacacacgc cacaccccgc cctgcccctg cggatcaggc acaagc
//
LOCUS       DAWEWP010000004        31465 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWEWP010000004 DAWEWP010000000
VERSION     DAWEWP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.004"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(55..333)
                     /locus_tag="WGN53_000106"
     CDS             complement(55..333)
                     /locus_tag="WGN53_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139155.1"
                     /translation="MRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVGKQLTDSVGLE
                     FDPYYRHKTICKPREIVLDGDRTKMGRSKSNEYGFRVTATFYSQLKSK"
     gene            complement(422..627)
                     /locus_tag="WGN53_000107"
                     /pseudo
     CDS             complement(422..627)
                     /locus_tag="WGN53_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            979..2034
                     /gene="aroG"
                     /locus_tag="WGN53_000108"
     CDS             979..2034
                     /gene="aroG"
                     /locus_tag="WGN53_000108"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ0139156.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            2102..2590
                     /locus_tag="WGN53_000109"
     CDS             2102..2590
                     /locus_tag="WGN53_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ0139157.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(2940..3416)
                     /locus_tag="WGN53_000110"
     CDS             complement(2940..3416)
                     /locus_tag="WGN53_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ0139158.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(3428..3856)
                     /locus_tag="WGN53_000111"
     CDS             complement(3428..3856)
                     /locus_tag="WGN53_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ0139159.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSDMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLILLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            3968..4213
                     /locus_tag="WGN53_000112"
     CDS             3968..4213
                     /locus_tag="WGN53_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139160.1"
                     /translation="MLKSIEFNSHIRNRLAAYLKGRGMDFQTAMREEKGNKEIASIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            5406..7508
                     /locus_tag="WGN53_000113"
                     /pseudo
     CDS             5406..7508
                     /locus_tag="WGN53_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            7750..9216
                     /locus_tag="WGN53_000114"
     CDS             7750..9216
                     /locus_tag="WGN53_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0139161.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(9417..10385)
                     /locus_tag="WGN53_000115"
     CDS             complement(9417..10385)
                     /locus_tag="WGN53_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139162.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAYLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            10462..11754
                     /locus_tag="WGN53_000116"
     CDS             10462..11754
                     /locus_tag="WGN53_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ0139163.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            complement(12257..12943)
                     /locus_tag="WGN53_000117"
     CDS             complement(12257..12943)
                     /locus_tag="WGN53_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ0139164.1"
                     /translation="MYAFTAAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(13018..13491)
                     /gene="queF"
                     /locus_tag="WGN53_000118"
     CDS             complement(13018..13491)
                     /gene="queF"
                     /locus_tag="WGN53_000118"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ0139165.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFETLARKRLFEHDSQ"
     regulatory      complement(13495..13539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            13768..14940
                     /gene="farA"
                     /locus_tag="WGN53_000119"
     CDS             13768..14940
                     /gene="farA"
                     /locus_tag="WGN53_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ0139166.1"
                     /translation="MATHGDEVKIQNRQEADSAKAKRKRCLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELIQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     gene            14964..16490
                     /gene="farB"
                     /locus_tag="WGN53_000120"
     CDS             14964..16490
                     /gene="farB"
                     /locus_tag="WGN53_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ0139167.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            16740..16973
                     /gene="rpmB"
                     /locus_tag="WGN53_000121"
     CDS             16740..16973
                     /gene="rpmB"
                     /locus_tag="WGN53_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ0139168.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            17005..17160
                     /gene="rpmG"
                     /locus_tag="WGN53_000122"
     CDS             17005..17160
                     /gene="rpmG"
                     /locus_tag="WGN53_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ0139169.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(17439..17747)
                     /locus_tag="WGN53_000123"
     CDS             complement(17439..17747)
                     /locus_tag="WGN53_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139170.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDYGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(17731..18033)
                     /locus_tag="WGN53_000124"
     CDS             complement(17731..18033)
                     /locus_tag="WGN53_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ0139171.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(18107..20470)
                     /locus_tag="WGN53_000125"
     CDS             complement(18107..20470)
                     /locus_tag="WGN53_000125"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ0139172.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAETAAPAFAG
                     VVVAEVKSVEKHPDADRLNVTRVDAGTGELVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVNGLHILPEDAPVGINIREYLDLDDTLFT
                     LKITPNRADCLSVKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISVLVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRSRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGYPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGM
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(20525..21550)
                     /locus_tag="WGN53_000126"
     CDS             complement(20525..21550)
                     /locus_tag="WGN53_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0139173.1"
                     /translation="MVSAIDSLKKNVNYNYVNSSNTHTIQIVHVEKPEGPIKIKRGDT
                     ETSISKQAIWRLADALSTGEPVNVDRVFGASYNFRSALEALLAHTPEIYWCKLQRIQP
                     GLNKSEVVEGHKHIIWLPDRPHEKGVMKEYETDVVISEIPSNNVVYDALSLPSTHSEI
                     DIDVKRRHVQIQIALSAIGFQLGFRTWIARNDQGISYGDKKIADLDGIVQKIESEKLI
                     SSFDGAVQAAMNIDCIWFRNGKLMPAVMEVEHSTGVRSGLARMKQLKDLLPPYANTRW
                     VIVAPDEDRDKVFKEANVPMFKNLDTQYFPYSAVEELYSLCIRRKLTNKAVNEEFLDC
                     FMEKCVM"
     gene            complement(21552..22646)
                     /locus_tag="WGN53_000127"
     CDS             complement(21552..22646)
                     /locus_tag="WGN53_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0139174.1"
                     /translation="MIFSFFSGAGFLDLGFELSGFDIAFVNEVHPPFLEAYKYSRSRM
                     DIPKPKYGYFKGSIDECLYAEKAKDLAGWVKKEKQNGIIVGFIGGPPCPDFSIAGKNK
                     GKDGENGKLSQSYVDLICKNQPDFFVFENVKGLYRTAKHREFFNALKRQLSDFGYVCT
                     EKLINAIEYGVPQDRERIILVGFLSQHVDALQKFDWDAHISFPDALEKDWPTTEEVGR
                     VVSQPANIYPELTVQYWFNRNGVDTHPNASKHFQPRAGLEKFQTISEGDDKKKSYKRL
                     HRWRYSPTAAYGNNEVHIHPYLPRRISAAEALAIQSLPKEFELPDNMTLSNMFKTIGN
                     GVPFLAAKGIAMTLKSYLENHYERTKTDGC"
     gene            complement(22717..23163)
                     /locus_tag="WGN53_000128"
     CDS             complement(22717..23163)
                     /locus_tag="WGN53_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ0139175.1"
                     /translation="MDRLTPEQRKKCMQSNKSKGTKPELALAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQRKAEIKGNREFWIAKIERNIRRDMEVTG
                     RLKVEGWAVLRFWSNDVVKNTTCCAEKVRQAVRDKQGLSKFQTREK"
     gene            complement(23487..24479)
                     /gene="pheS"
                     /locus_tag="WGN53_000129"
     CDS             complement(23487..24479)
                     /gene="pheS"
                     /locus_tag="WGN53_000129"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0139176.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSGEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFAK"
     gene            complement(24825..25184)
                     /gene="rplT"
                     /locus_tag="WGN53_000130"
     CDS             complement(24825..25184)
                     /gene="rplT"
                     /locus_tag="WGN53_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ0139177.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKSALAA"
     gene            complement(25197..25394)
                     /gene="rpmI"
                     /locus_tag="WGN53_000131"
     CDS             complement(25197..25394)
                     /gene="rpmI"
                     /locus_tag="WGN53_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ0139178.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(25541..26062)
                     /gene="infC"
                     /locus_tag="WGN53_000132"
     CDS             complement(25541..26062)
                     /gene="infC"
                     /locus_tag="WGN53_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ0139179.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(26080..27993)
                     /gene="thrS"
                     /locus_tag="WGN53_000133"
     CDS             complement(26080..27993)
                     /gene="thrS"
                     /locus_tag="WGN53_000133"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ0139180.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVAVKLSLRPEQRAGSDEVWDKAEQGLRDALTACGVE
                     WEELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(28046..28122)
                     /locus_tag="WGN53_000134"
     tRNA            complement(28046..28122)
                     /locus_tag="WGN53_000134"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28086..28088),aa:Val,seq:gac)
     gene            28301..29416
                     /gene="tgt"
                     /locus_tag="WGN53_000135"
     CDS             28301..29416
                     /gene="tgt"
                     /locus_tag="WGN53_000135"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ0139181.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQAKKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRTGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQARFHENRARGTD"
     gene            29426..30508
                     /locus_tag="WGN53_000136"
     CDS             29426..30508
                     /locus_tag="WGN53_000136"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ0139182.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            30700..31101
                     /locus_tag="WGN53_000137"
     CDS             30700..31101
                     /locus_tag="WGN53_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0139183.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(31164..31454)
                     /locus_tag="WGN53_000138"
     CDS             complement(31164..31454)
                     /locus_tag="WGN53_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139184.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         7728 a         8037 c         8016 g         7684 t
ORIGIN      
        1 gtatctgcga accgttttgt ctgattacgc ccgatttgac gatgcactac tgtcctactt
       61 tgattttaat tgactataga acgttgcggt tacgcggaag ccgtactcgt tggatttgga
      121 gcggcccatt ttggttctgt cgccgtccaa gacaatctca cggggtttgc agattgtttt
      181 gtgacggtag tatggatcaa actcgagacc gacgctgtcg gtcaactgtt tgcctacatt
      241 cagaccgata ccgacactcc aacctttggc gcttttgctg acatcgcggg aagcacacat
      301 ctgggtcgtc atcactttgg ttttgccgcg caaatctgca tatgcatccg cccaaggggt
      361 tagggatcat ccgtccccca aatcttggcg gatttcgcca tggactttca aagcaaggtt
      421 ttcatgcttg gtaacggtgt ttttccttat cgccgatgat ggctttgcct ttgccgttag
      481 actcgggaat atcggctacc gtaacggcgg acacggctgc aagtgagagt gcaagcaggg
      541 ttttttcatg tttttcttcc tataatgagg ataaataaat ggaaaaagtg tgggaaatac
      601 ccgcattccc attaaatctt ttttcaagca atgagttctt tttgttttca acattttcct
      661 tgtgtatgaa attttgccgg atgtgcaggc ggaatcggca gcggggtgtt ctgtaccgga
      721 ttgtcgtgga aatgggaaaa cggatgttcc gtgcggtttt gtccgaatga atggcgggta
      781 ttgtttttat caatctgttt ctttttcttt ggaataaaat tcctaaaata ttaaaaatat
      841 gaaatttaaa atctatgaaa aaagatatat cagttatttt gaaataaaat agctttgtag
      901 taatatgttg cacttgtttg tgcaaggtaa acgatgtaac ctaagccgcg tataaaaacc
      961 catcaggaaa gatgcaagat gacacaccat taccccacag acgatattaa gattaaagaa
     1021 gttaaagagt tgttgccgcc gattgcccat ctttacgagc tgccgatttc caaagaggct
     1081 tcgggcttgg ttcaccgcac ccgtcaggaa atttccgatt tggttcacgg cagggacaag
     1141 cggctgttgg ttattatcgg gccgtgttcg attcacgatc cgaaagcggc gttggaatat
     1201 gcggagcgtt tgttgaaact ccgcaagcag tatgaaaacg agcttttgat tgtgatgcgc
     1261 gtttatttcg agaagccgag gacgacggtg ggttggaaag gtttgattaa cgacccgcat
     1321 ttggacggta cgtttgacat caatttcggt ttgcgtcagg cgcgcagcct gttgttgtcg
     1381 ctgaacaata tgggtatgcc tgcctctacc gagtttttgg atatgattac gccgcaatat
     1441 tatgcggact tgatttcttg gggggcaatc ggtgcgcgga cgaccgaaag ccaagttcac
     1501 cgcgaattgg caagcgggct gtcctgcccc gtcggcttta aaaacggtac ggacggcaat
     1561 ttgaagattg ccatcgacgc aatcggtgcg gcgagccatt cgcatcattt cttgtctgta
     1621 accaaggccg gacattccgc cattgtccat accggcggca atcccgactg tcatgtcatt
     1681 ttgcgcggcg gaaaagagcc gaattatgat gcgggacacg tcagcgaggc ggcggaacaa
     1741 ctgcgtgcgg caggggtaac cgataagctg atgatagatt gcagccacgc caacagccgc
     1801 aaggattaca cccggcagat ggaagtggca caagatattg ctgcccaatt ggaacaggac
     1861 gggagcaata ttatgggcgt gatggtggaa agccatttgg tcgaaggcag acaggataag
     1921 ccggaagtgt acggcaaaag cattaccgat gcgtgtatcg gttggggcgc gactgaagaa
     1981 ctgttggcat tgttggcagg tgcaaacaaa aaacgtatgg cgcgcgccgg ttgagatttt
     2041 tgacacggaa tgtcataaaa tgccgtctga agcgttcaga cggcattttt gtggaggaaa
     2101 tatgctcaaa ataaccctaa ttgcggcgtg tgcggaaaac ctgtgcatcg gggcgggcaa
     2161 tgctatgcct tggcacatcc ccgaagattt cgcatttttc aaagcctata ccttgggcaa
     2221 acccgtcatt atggggcgga aaacgtggga atccctgccc gtcaaacccc tgcccggacg
     2281 gaggaacatc gtcatcagcc ggcaggcgga ttattgcgcg gcaggcgcgg aaacggcggc
     2341 aagtttggag gcggcattgg cattgtgcgc aggcgcggaa gaagccgtca ttatgggcgg
     2401 cgcgcagata tacggacaag cgatgccatt ggcgaccgat ttgcggataa ccgaagtgga
     2461 tttgtctgtg gaaggagatg catttttccc cgcaatagac cggacgcatt ggaaagaagc
     2521 agagcggacg gaacgccgtg tcagcagcaa aggcacgagc tatgcttttg tgcattattt
     2581 gagatattga aatataaact ctctataaaa tcccccgcaa atgatgggct gaaatagaaa
     2641 atattgttat tcccccgaag atgggaatcc gggattttaa agttagggta atttatccga
     2701 aataacaaca atcttccatc gtcattcccg caaaagcggg aatccggaaa cgaaaagcta
     2761 aagcaattta tcggaaaaaa ccgaagttta aagaaccgga ttcccgcctg cgcgggaatg
     2821 acgagatttt aggttatggg gatttattgg gaataatgga acaaagaaag cagaaataag
     2881 gatatagagg ctgtctttgg atttgcgatg gttgtcggag aatgccgtct gaagccgttt
     2941 cagacggcat ttttccagct tgagaacgga tgcctgctca aataagcatt ggtaaacata
     3001 ccgtcggcag tgatttcccg tcccagccag tccggacggt caaaatcggc attctcgtcg
     3061 ggcaactcga tttccgcgac gaccaaaggc gcattatcgc caagaaaaac atcgatttca
     3121 aacaggctgc cgccccatct gaccggataa cgccattttt ccattttaaa cgggcacatc
     3181 gtttccatca tcttttccgc atcggcaagc gggatttcgt attcaaactc actgcggctg
     3241 atttccgaaa tatagccttt cagcgtcagc cacgcccgtt gtccggcaat gcggacacgg
     3301 acggtgcgtt ctttttcaac agacagataa ccctgcctca acagcagcgg ttcgtcggcg
     3361 tattgccgcc agttgtcgtt tccaatcaaa aaacggcgtt cgatttctat cggcataaga
     3421 tgctccgtca aaacggtttg aacacgacca gatacagcgc ggcaaccatc agcagcacgg
     3481 ggatttcgtt gaacacgcgg taccagcggt gtgaaaaagc attgctgtaa tcctgaaaac
     3541 ggcgcagcag cacgccgcaa tacaactggt aagccaagag tatcaagccc aaacacagtt
     3601 tgacgtgtac ccagccgctg ccccaccagc cggcggcaaa cggtatcgcc gcgccgaaca
     3661 cgaccgcgcc gaagcccaac ggcgacataa aacggtacag ccgcaccgcc atatccgaca
     3721 ggcgcacata ctcgggattg ccgcgcggtg catcaatcat cgccatattg acgaaaatcc
     3781 tcggcaggta aaacagccct gcaaaccacg aaatgacaaa aaacaagtga aacagcttga
     3841 accaagaaaa catcatcgcc cacaccctgc cgaaaagcgg tattgtacag gcaaaccgct
     3901 tgggaaacgt gataaaatca ggcggataaa caaatcgaat aaatccttac cgcaaaacgg
     3961 aggcaaaatg ctcaaatcca tcgaattcaa ttcccacatc cgcaaccgcc ttgcagcata
     4021 tctgaaaggc aggggtatgg attttcagac ggcgatgcgg gaggaaaaag gcaacaaaga
     4081 aatcgcctcc atcgtccaca gcggtctgcc cactctggtc cgcaaactgt attccgaaca
     4141 aaaaatgcag aagttttttt gggaaaagcg ggatttgatt gccgactaca tcagccgccg
     4201 gatgcaggga taggcggctg aaatctgttt tcaggcaagt gaaaagacaa tatggcagat
     4261 tgaaattatg cttatcgtca ttcccgcccg cgcgggaatc cgacttgttt ggtttcggtt
     4321 atttttcgtt tcgtaacttt tgagccgtca ttcccgcgca ggcggtaatc cggcttgttc
     4381 ggtttcggtt ctttttctcg tttcgggtga tttctaaacc gtcattcccg cgcaggcggg
     4441 aatctaggtc tttaaacttc ggttttttcc gataaatttt tgccgcatta aaattctaga
     4501 ttcccacttt cgcgggaatg acggcggagg gtttttagtt ttcccgaaaa tgcacatcat
     4561 ccaaaatccc gttattccca caaaacagaa aatcaaaaac agtaacctga aatcccgtcc
     4621 ttccctcccg tgcgggaatc cgacttatct ggtttcggtt ctttttctcg tttcaggtga
     4681 tttctaaacc gtcattcccg cgcaggcggg aatccagacc ttcaaacccc ggacatcccc
     4741 gataaattct tgcggcattg aaattctaga ttcccgcttt cacgggaatg acggcggagg
     4801 gtttttagtt ttcccgataa atgcacatca tccaaaatcc cgttattccc acaaaacaga
     4861 aaatcaaaaa caacaatctg aaattccgtc cttcccgcgc aggcggtaat ctgaacacgt
     4921 ccgtagtgaa acctatatcc cgtcattcgc acgaaagtgg gaatccagga tgcagggaaa
     4981 accgttttat ccgataagtt tccgcaccga aaggtctaga ttcccgcttt cgcgggaatg
     5041 acggcggagg gtttttagtt ttctcgataa atgcacatca tccaaaatcc cgttatttcc
     5101 acaaaacaga aaatcaaaaa cagtaacctg aaatcccgtc attcccgcgc aggcgggaat
     5161 ccggcttgtt cggtttcggt tctttttctt gtttcgggtg atttctaaac cgtcattccc
     5221 gcgcaggcgg gaatccagac ctttaaaccc cgaccatcct tgataaattc ttgcggcatt
     5281 aaaattctag attcccgctt tcgcgggaat gacggcggag ggttttttgc ttttcctgat
     5341 ttttcattgc gatgtagtat aatgtagtat ataatcatta taattttaac acttgacaaa
     5401 ggaaaatttc tcatgacact gaaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa
     5461 gcggtcgggt taatctatta acattatctg ttttatcgct gttttgcacg ccatatgttt
     5521 gaggttcgga tgcgtacgat cccgtcaaag aagccgagat taaaaacaaa tttattttag
     5581 aagcggcgga agacagaaat tcccacgttt ggcgcggccc gtgcagcata tcttttgatt
     5641 gcttcggtat gttcagagct cagcttggtt caaatactcg ttctaccaaa atcggcgacg
     5701 atgccgattt ttcattttca gacaagccga aacccggcac ttcccattat ttttccagcg
     5761 gtaaaaccga tcaaaattca tccgaatatg ggtatgacga aatcaatatc caaggtaaaa
     5821 actacaatag cggcatactc gccgtcgata atatgcccgt tgttaagaaa tatattacag
     5881 atacttacgg ggataattta aaggatgcgg ttaagaagca attacaggat ttatacaaaa
     5941 caagacccga agcttgggaa gaaaataaaa aacggactga ggaggcgtat atagaacagc
     6001 ttggaccaaa atttagtata ctcaaacaga aaaaccccga tttaattaat aaattggtag
     6061 aagattccgt actcactcct catagtaata catcacagac tagtctcaac aacatcttca
     6121 ataaaaaatt acacgtcaaa atcgaaaaca aatcccacgt cgccggacag gtgttggaac
     6181 tgaccaagat gacgctgaaa gattcccttt gggaaccgcg ccgccattcc gacatccata
     6241 cgctggaaac ttccgataat gcccgcatcc gcctaaacac gaaagatgaa aaactgaccg
     6301 tccataaagc gtatcagggc ggcgcggatt tcctgttcgg ctacgacgtg cgggagtcgg
     6361 acgaacccgc cctgaccttt gaacaaaacg tcagcggaaa atccaatgtg gttttggaac
     6421 gccggccgga aaatctgaaa acgctcgacg ggcgcaaact gattgcggcg gaaaaggcag
     6481 accctaattc gtttgcgttt aaacaaaatt accggcaggg actgtacgaa ttattgctca
     6541 agcaatgcga aggcggattt tgcttgggcg tgcagcgttt ggctatccct gaggcggaag
     6601 cggttttata tgcccaacag gcttatgcgg caaatacttt gtttgggttg cgtgccgccg
     6661 acaggggcgg cgacgtgtat gccgccgatc cgtcccgtca aaaattgtgg ctgcgcttca
     6721 tcggcggtcg gtcgcatcaa aatatacggg gcggcgcggc tgcggacggg cggcgcaaag
     6781 gcgtgcaaat cggcggcgag gtgtttgtac ggcaaaatga aggcagccgg ctggcaatcg
     6841 gcgtgatggg cggcagggcc ggccagcacg catcagtcaa cggcaaaggc ggtgcggcag
     6901 gcagttattt gcatggttat ggcgggggtg tttatgctgc gtggcatcag ttgcgcgata
     6961 aacaaacggg tgcgtatttg gacggctggt tgcaatacca acgtttcaaa caccgcatca
     7021 atgatgaaaa ccgtgtggaa cgctacaaaa ccaaaggttg gacggcttct gtcgaaggcg
     7081 gctacaacgc gcttgtggcg gaaggcgttg tcggaaaagg caataatgtg cggttttacc
     7141 tgcaaccgca ggctcagttt acctacttgg gcgtaaacgg cggctttacc gacagcgagg
     7201 ggacggcggt cggactgctc ggcagcggtc agtggcaaag ccgcgccggt attcgggcaa
     7261 aaacccgttt tgctttgcgt aacggtgtca atcttcagcc tttcgccgct tttaatgttt
     7321 tgcacaggtc aaaatctttc ggcgtggaaa tggacggcga aaaacagacg ctggcaggca
     7381 ggacggcgct cgaagggcgg ttcggcattg aagccggttg gaaaggccat atgtccgcac
     7441 gcatcggata cggcaaaagg acggacggcg acaaagaagc cgcattgtcg ctcaaatggc
     7501 tgttttgatg cgtcgggaaa tgttttgacg cacaggcggc acaccggcac ggccccgcgc
     7561 gccgccccgc aaaccaatcc gaaccctgcc gccccgaagg gcggggcatc atgatgaaac
     7621 cggcggaaaa ccgccggttt tttgccgccg tttgaaaccc gattctggct tcagacggca
     7681 ttgtcgcggc atcgggcggc agggtttgga acagcggcat aaaaaactga tacaatccgc
     7741 cgattgataa tggttatttt ttatttttgt gggaagacat ttatgcctgc acgaaacaga
     7801 tggatgctgc tgctgccttt attggcaagc gcggcatatg ccgaagaaac accgcgcgaa
     7861 ccggatttga gaagccgtcc cgagttcagg cttcatgaag cggaggtcaa accaatcgac
     7921 agggagaagg taccggggca ggtgcgggaa aaaggaaaag ttttgcagat tgacggagaa
     7981 accctgctga aaaatcccga attgttgtcc cgcgcgatgt attccgcagt ggtctcaaac
     8041 aatattgccg gtatccgcgt tattttgccg atttacctac aacaggcgca gcaggataag
     8101 atgttggcac tttatgcaca agggattttg gcgcaggcag acggtagggt gaaggaggcg
     8161 atttcccatt accgggaatt gattgccgcc caacccgacg cgcccgccgt ccgtatgcgt
     8221 ttggcggcgg cattgtttga aaacaggcag aacgaggcgg cggcagacca gttcgaccgc
     8281 ctgaaggcgg aaaacctgcc gccgcagctg atggagcagg tcgagctgta ccgcaaggca
     8341 ttgcgcgaac gcgatgcgtg gaaggtaaat ggcggcttca gcgtcacccg cgaacacaat
     8401 atcaaccaag ccccgaaacg gcagcagtac ggcaaatgga ctttcccgaa acaggtggac
     8461 ggcacggcgg tcaattaccg gctcggcgcg gagaaaaaat ggtcgctgaa aaacggctgg
     8521 tacacgacgg ctggcggcga cgtgtccggc agggtttatc cggggaataa gaaattcaac
     8581 gatatgacgg caggcgtttc cggcggcatc ggttttgccg accggcgcaa agatgccggg
     8641 ctggcagtgt tccacgaacg ccgcacctac ggcaacgacg cttattctta caccaacggc
     8701 gcacgccttt atttcaaccg ttggcaaacc ccgaaatggc aaacgttgtc ttcggcggag
     8761 tgggggcgtt tgaagaatac gcgccgggcg cgttccgaca atacccattt gcaaatttcc
     8821 aattcgctgg tgttttaccg gaatgcgcgc caatattgga tgggcggttt ggatttttac
     8881 cgcgagcgca accccgccga ccggggcgac aatttcaacc gttacggcct gcgctttgcc
     8941 tgggggcagg aatggggcgg cagcggcctg tcttcgctgt tgcgcctcgg cgcggcgaaa
     9001 cggcattatg aaaaacccgg ctttttcagc ggttttaaag gggaaaggcg cagggataaa
     9061 gaattgaaca catccttgag cctttggcac cgggcattgc atttcaaagg catcacgccg
     9121 cgcctgacgt tgtcgcaccg cgaaacgcgg agtaacgatg tgttcaacga atacgagaaa
     9181 aatcgggcgt ttgtcgagtt taataaaacg ttctgattgc tgttcctttt cggaggaaac
     9241 cctgccggcg gcggtatcac ggcgggcatc ggcggctttc gggcggtgct ttgcgtgccg
     9301 ccgcgtgtgc ggaaacgcat tccggttttt ccggcataac ggcgatgcga ggtaaaatgc
     9361 cgtctgaaac ccgattcggg cttcagacgg cattgtcgcg gttgcggcgg gcgggttcac
     9421 cagattccgt caaaggtttt cgcgccgcgc caaaatttcc acctgtcggc gggtttgaag
     9481 gtcagcgtac cgccgtgttg tccgtccgtg gcgatgtcca gccgtttgat tttgccgatg
     9541 cggacggctt cgtaaagcgg tgcgaaccag cgttcttccc actgctgcaa tattgccgca
     9601 taccgctccc tgtcccctgt cagggcggtc aggcgcaaat cgtccataaa caggatatgg
     9661 tgcgtgtcgg gtaggtatgc cgccgtttct tcataggcgc ggaagttgtc gggcaatgcg
     9721 cggcggtcgg ggtggaaacg gctccaaacc gtatcggcga aaagcgtgcc gccttgactg
     9781 cttccgtgca gactgtccca aagccataag ccgttcaact cgggcagccc gcgtttcttg
     9841 cggttatggt tgacggggtg cgccgccagc cacatttgga tttcggtttg gacgcgcagc
     9901 cattccaacg cgtcttcccc gtccggctga tcgtccgcgc ccaacaatcc gcccaagtcc
     9961 aaaacgggct tcgcgcccca gcggtacgcg caaggaaggg aaaccagcca taattcgggc
    10021 aggacgggaa cgaaacgcca tggaatgtcg ccgtaaaacg ccgacaggtc gcggcagagc
    10081 cgttccgctt catccgtacc gacgttcaga tattccgccg ttagcacatt tgcctgatgc
    10141 atccccatct tttgccagac gggcgtggcg agcgcgacgg tttcagacgg catattcagg
    10201 ctttgcgccg cgcgttccgc cagtctgccg caccacaaat aacgcgcgta aaatgccgaa
    10261 gccgtgcagc tttggcggtg cagcgagccg tattgcagga ttttgttgaa ggcgggcagg
    10321 catagaggta ttcggatttc gtcttcatcc aaattgagcg aggggagggc gagggtgagt
    10381 ttcatcgttt gatgtctgac aaatgcggat cgggcggata ttatagagga ttcggcgcaa
    10441 acgccgtcaa aaaggaacaa tatggctgtc ttcccacttt cggcaaaaca tcggaaatac
    10501 gcgctgcgcg cgcttgccgt ttcgattatt ttggtgtcgg cggcatacat tgctttgaca
    10561 gagaggacgg aacgcgtcag accgcagcgc gtggaacaaa atctgccgcc gctgtcttgg
    10621 ggcggcagcg gcgttcagac ggcatattgg gtgcaggagg cggtgcagcc gggcgactcg
    10681 ctggcggacg tgctggcgcg ttcgggtatg gcgcgggacg agattgcccg aatcacggaa
    10741 aaatatggcg gcgaagccga tttgcggcat ttgcgtgccg accagtcggt tcatgttttg
    10801 gtcggcggcg acggcggcgc gcgcgaagta cagtttttta ccgacgaaga cggcgagcgc
    10861 aatctggtcg ctttggaaaa gaaaggcggc atatggcggc ggtcggcttc tgaggcggat
    10921 atgaaggttt tgccgacgct gcgttcggtc gtggtcaaaa cgtcggcgcg cggttcgctg
    10981 gcgcgggcgg aagtgcccgt cgaaatccgc gaatccttaa gcgggatttt cgccggccgc
    11041 ttcagccttg acggtttgaa ggaaggcgat gccgtgcgcc tgatttacga cagcctgtat
    11101 ttccacgggc agcaggtggc ggcgggcgat attttggcgg ctgaagtcgt taagggcggc
    11161 acaaggcatc aggcgttcta ttaccgttcg gacaaggaag gcggaggggg cggcaattat
    11221 tatgatgaag acggcagggt gttgcaggaa aaaggcggct tcaacatcga gccgctggtc
    11281 tatacgcgca tttcttcgcc gttcggctac cgtatgcacc ccatcctgca cacttggcgg
    11341 ctgcacacgg gcatcgatta tgccgcaccg cagggaacgc cggtcagggc ttccgccgac
    11401 ggcgtgatta cctttaaagg ccggaagggc ggatacggca acgcggtgat gatacgccac
    11461 gccaacggtg tggaaacgct gtacgcgcac ttgagcgcgt tttcgcaggc acaaggcaat
    11521 gtgcgcggcg gcgaggtcat cggttttgtc ggttcgaccg ggcgttcgac ggggccgcac
    11581 ctgcattacg aggcgcgcat caatgggcag cccgttaatc ctgtttcggt cgcattgccg
    11641 acacccgaat tgacgcaggc ggacaaggcg gcgtttgccg cgcagaaaca gaaagcggac
    11701 gcgctgcttg cgcgcttgcg cggcataccg gttaccgtgt cgcaatcgga ttgaagtttg
    11761 aaccggcgac gaaaacaatg ccgtctgaaa acctgcaaac aggttttcag acggcattta
    11821 tagtggatta acaaaaacca gtacgtcgtt gcctcgcctt agctcaaaga gaacgattct
    11881 ctaagatgct gaagcaccaa gtgaatcggt ttcgtactat ttgtactgtc tgcggcttcg
    11941 tcgctttgtc ctgatttttg ttaatccacc atacagtcga ttaaaataaa actaagttaa
    12001 ggaagcactg ccgtcattcc cgtaccgtcg ggaatccgga caccacggca cggaaaccca
    12061 tccgccgtca ttcccgcgaa agcgggaatc tagaaataca atgcggtagg agtttatcgg
    12121 aaatgactga aacccaacgt accggattcc cgctttcgcg ggaatgacga agtgggcggg
    12181 aatccggatt tatccgttcc gacagtgttt gcaaataaaa gaaaacccaa ccgtcccgat
    12241 tcccggcagg gctgttttac ggattttgca gcgagggcgc ggggcggtct tgcgcctgtt
    12301 tggtttgcag ggttgtcagt tttttcgtca gcagattcag aatcacgccg taggcgggca
    12361 ggaaaaacag accgcagacg gtaagtttga acaggtaatc gacaaaagcg atgccctgcc
    12421 agtttgccgc cataaattca tcgctgctgg cgtaaaaggc aacggcaaaa aacactaacg
    12481 tgtccagtgc attgccgatg acggttgatg cggtcggtgc aatccaccac gctttcagac
    12541 gacgtaattt gttgaacaca aaaatatcaa ggatttgtcc gagcgcgtag gcggcaaagc
    12601 tggccaaggc gatgcgtccg acaaaggtgt tgaattcgga tagtgcgccc aagcctgtcc
    12661 aactgccgtt gtggaacaaa acggaaaaga cgtaggaaag caaaagggcg gggaacatca
    12721 cccaaaagat aatccgccgt gccaagtgcg aaccgaaaat gcggacggtc aggtcggtgg
    12781 cgaggaagat gaagggaaag gaaaacgcac cccaagtggt gtggatgccg aaaatttgga
    12841 aggggaactg caccagatag ttgctggcgg cgatgatgag gatatggaaa agcaccagcc
    12901 agaagagtgc cttctgttgc tgtgcggcag taaatgcgta cataaaaatc tttcggaaag
    12961 gcgttcagac ggcatatcgt atcgaaggaa tgccgtctga aatatgggaa ggatggttta
    13021 ttggctgtcg tgttcgaaca agcgtttacg cgccaatgtt tcgaactcgg tgcctgcttt
    13081 gccgtaattg gcgaaaggat gaatggcgat gccgccgcgc ggtgtgaact cgccgaatac
    13141 ttcgatgtat ttcggatcca tcagggcaat gaggtctttc atgatgatgt tgacgcagtc
    13201 ttcatgaaaa tcgccgtggt tgcggaagct gaagaggtag agtttcaggg atttgctttc
    13261 caccattttg atgtgcggaa tgtagcggat gtagatggtg gcgaagtcgg gctgcccggt
    13321 catggggcag aggctggtga actctgggca gacgaatttg acgaaatagt cgttgtcggg
    13381 atgtttgttg tcgaatgctt cgagaatttc aggcgcgtag ccggtcggat atcgggtttt
    13441 ttgattaccc aaaagcgaga tgccttgcag ctcttcggta ttgcgggaca tagggtttcc
    13501 ttagtttttt agtgtgggag gttttcgaac cacggggcga cgattgtaat ataagcggcg
    13561 gtatctgtgt agcgtccccg cccggtacgg cgcgcttatc cgattatttt tgaacataaa
    13621 ttaattttgc ggaacgcggg tgtggcggtt tatttgaata ttcattctaa atcattaact
    13681 tgattgggtt attgccaggg tatagaatcc gagctgcctt tgatcggctt tatttatatc
    13741 gtattaaaaa tattcggaat tggaataatg gcaacacacg gagacgaagt gaaaattcaa
    13801 aacagacaag aagccgatag cgcaaaagcc aaacgcaaac gctgcctgac ggcattgacg
    13861 ctgctgttcg cgcttgccgc cgcagccgcc gggtcggcgt tttttttatg gtggcagcac
    13921 gaagaggaaa cggaagacgc ttatgttgcc ggacgcgtgg ttcaggttac gccgcaaaag
    13981 ggcggtacgg tgcggaaggt tttgcacgac gatacggatg ccgtgaaaaa aggcgacgtg
    14041 ctggcggtat tggacgacga taatgatgtg ctggcttacg agcgggcaaa aaacgagctg
    14101 attcaggcgg tgcggcaaaa ccgccggcaa aatgccgcca cttcgcaggc gggggcgcag
    14161 gttgccttgc gccgggcgga tttggcacgc gcgcaggatg atttgcgccg ccggtctgct
    14221 ttggcggaat cgggcgcggt gtccgccgaa gagctggcac atgcccgtgc ggcagtgtct
    14281 caggctcagg cggcagttaa ggcggctttg gcggaagaat cttcggcgcg tgcggctttg
    14341 ggcggtcagg tttctttgcg cgaacagccg gcggttcaga cggcaatcgg caggttgaaa
    14401 gatgcgtggt tgaaccttca gcggacgcaa atccgcgcgc cggcggacgg tcaggtggcg
    14461 aagcgttcgg tgcaggtcgg gcagcaggtg gcggcaggcg cgccgctgat ggcggtggtg
    14521 ccgctgtcgg atgtgtgggt ggatgctaat tttaaagaga cgcagttgcg gcatatgaaa
    14581 atcggacagc ctgccgagct ggtgtccgat ttgtacggca aacaaattgt ttatcgcggc
    14641 agggtggcag gtttttcggc aggtacgggc agcgcgtttt cgctgattcc ggcgcaaaac
    14701 gcaacgggca actggattaa agtggtgcag cgcgtccccg tccgtatcgt gctgaaccgc
    14761 gaagatgtgg acaggcatcc gttgcgtatc ggtttgtcga tgacggttaa agtggatact
    14821 tccgccgcag gcgcgcctgt ttcaaaaacg ccgggtgcgg cattgccgga aatggaaagt
    14881 accgactggt cggaagtcga tcggacggtc gatgaaatcc tcgggcaatc cgcgccctga
    14941 tgccgtctga aacggaggac acaatggatt atccaccgct taagggtgcg gcattggcgt
    15001 gggttacgct gtctttgggg cttgccgtat ttatggaagt tttagatacg actatcgcca
    15061 atgtcgccgt tcccgtcatc gccggcaacc tcggtgcggc aaccacgcag gggacgtggg
    15121 tcattacttc ttttgctgtg gcaaacgccg tttccgtgcc gttaacgggc tttttggcaa
    15181 aacgtatcgg cgaggtcaaa ttgtttaccg ccgccgctgt cggtttcgtc atcacatcgt
    15241 ggctgtgcgg tattgccccc aaccttcagt cgctggttgt tttccgcatc ttgcagggct
    15301 ttatcgccgg gccgctgatt cccctgtcgc aaagcctgtt aatggcatcc tatccgcccg
    15361 caaaacggac gctggcactg gcattgtggg caatgaccgt cgttgtcgcc cctgttctcg
    15421 ggccgatact cggcggttgg atttccggaa actggcattg gggttggatt ttcttcatta
    15481 atatccctat cggtatcata tcggcatgga ttacatggaa acatttgaaa tatcgggaaa
    15541 cggaaaccgt taaaatgccg accgactatg tcgggcttac attgatggta gtcggtatcg
    15601 gcgcgttaca gatgatgctg gacaggggta aggaactcga ctggttcgcc tctggagaaa
    15661 tcattacctt gggcgtagtc gcactggtgt gtttgtcgta ttttattgtt tgggaattgg
    15721 gagaaaaata tccgattgtc gatttatcgc tgtttaaaga tcggaatttt accgtcggcg
    15781 taattgccac atcattgggt tttatggtgt atatggggac gctgaccttg ctgccgttag
    15841 tgttgcagac caacctgggc tatacctcca cgtgggcggg gcttgccgcc gcacccgtcg
    15901 gcatcctgcc cgtcttcctg tctccgttaa tcggcaggtt cggcaataaa atcgatatgc
    15961 gcctgctcgt aaccgccagc ttcctgacct ttgcctttac tttctattgg cgcaccgatt
    16021 tttatgccga catggatatc ggcaacgtca tctggccgca gttttggcag ggggcaggcg
    16081 ttgccatgtt cttcctgccg ctgaccacca tcacactgtc gcacatgaag ggcggacaga
    16141 ttgccgccgc aggcagcctg tcgaatttct tgcgcgtgct gatgggcggt gtcggcgtat
    16201 ccgtcgtcag caccctgtgg gaacggcgcg aagcgttgca ccatacacgc tttgccgaac
    16261 acatcacgcc ctattccgca acattgcacg aaacggccgc tcatttgtcc cagcacggcg
    16321 tttccgacat tcaaacccta ggcatcatca acaataccat tacccagcag ggttttatta
    16381 tcggctcgaa cgaaatcttt atggcgggca gcttgttatt cattatcatg atacccgtca
    16441 tatggctggc aaaaccgccg ttccacaacg gcggcggcgg tggacattga gggatttgaa
    16501 aacttgaaat gccgtctgaa aatactggaa atatgttcgg acggcatttt gaatgcagca
    16561 gttcccgaaa tccgctataa tcgcgcccca tctgtttcgc acctgcaaac gttccacaga
    16621 tgcgacaatc ggaaggatta tccgcgcaaa acagccgttt ttctctaaaa cacttgaact
    16681 aacactgttt ttcgtggtat aaatcgcgtt ttactatttt agaagtttgg agactgatta
    16741 tggcacgagt ttgcaaagtg accggcaaac gcccgatgtc cggcaacaac gtatcgcacg
    16801 ccaacaacaa aaccaaacgc cgttttttgc ccaacttgca atcacgtcgt ttttgggtag
    16861 aaagtgaaaa ccgctgggtt cgcctgcgcg tttccaacgc tgcactgcgt accatcgaca
    16921 aagtaggcat tgatgtcgta ttggctgatt tgcgtgctcg cggcgaagct taatttaaac
    16981 actatttaat taaggattac tgcaatgcgc gataaaatca aactggaatc cagtgcaggt
    17041 actggtcact tctacaccac taccaaaaac aaacgcacta tgcccggcaa actggaaatc
    17101 aaaaaattcg atccggttgc ccgcaaacac gtagtgtaca aagaaaccaa actgaaataa
    17161 tttcagtttg aaagcaaagc ctccgactgt tcggaggctt ttttattttt atcgtgtttc
    17221 ttttccgctt gaaacatctg ctgtatgcga atctgctgca aaccgtctgc caaggataga
    17281 gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat ttgtactgtc
    17341 tgcagcttcg tcgccttgtc ctgatttttg ttaatccact ataattattt tttagcgtgt
    17401 aaaacaaaac cggctgcata cctgcaaccg gcctcaaatc agcacaattc cttatccaaa
    17461 tccgccaaca gctctttcag accgtctccg atttcctgaa gcgtaaccgg acggttgccg
    17521 tccgaacccg actccgcacc ttctgcatac ggtgcaaacg tgctctgcaa tttcaacagt
    17581 ttcaccacat ccaaacgggt gtagcgttcg ccgccgtaac cgacaaccac accataatca
    17641 tgctgccatt gcgcaaaacc ataggggctg atttgcaaca gtccgcacaa ctcgtccagc
    17701 gtgaaatagc gttttgcggg aaccttcaaa tcaacgttgt ttgtcatagt agtgttccac
    17761 catgcttttg agtttctggc tggcatggaa agttaccacg cggcgggcgg taatcggcac
    17821 ttcctcgccg gttttaggat tacgacccgg gcgttgcggc ttgtcgcgca actggaaatt
    17881 tccgaaaccg gaaattttga tttcttcgcc gcttgccaaa gtgctgcgga tttcttcaaa
    17941 aaagagttcg acgatttctt tggcatcgtt tttggtgacg ttgctgactt tgtctaccaa
    18001 aatatcggcc agttctgctt tagtgagagt catatgatta ccttctgttg taacagttgt
    18061 ggattattac caatttttat ttaaaattca agcgaatatt tattttttaa ctgcgaagcc
    18121 gcgcccctgc cgccgttgcc gcgccaatca gttttccgat aagcggctcg accgcctcat
    18181 ccgtcagcgt gttttccata tcctgcaaaa tcactttgac cgccacgctc ttcatccctt
    18241 cgggcagtcc catgccgcga tacacgtcaa acacgctgat ttcctgtacc aacttgtttg
    18301 ccgcgccttt caagacaagc agcaaatcat catggctcat agcttccggc atcacaaacg
    18361 ccaaatcgcg gcgcaccggc tggaatttcg atacggcccg atagcgcgtt ttcccgcatt
    18421 ccaacacggc cgccatatcg atttcaaata ccagcggcgc ttgcggcagg tcgtattttt
    18481 gcagccattt cggatgcagt tcgccgacaa agccgatgac tttgccgtct gaaacgatat
    18541 tggcggcgcg tccgggatgc agggcgggat atccggtttt aacgaactcg accgctttgt
    18601 ttttcaacag attttccaca tctgccttga tgtcgtaaaa atccgcattg cgcgttttcc
    18661 cgccccattg ttcgggcatt gccgcgccgt accacaagcc gccgatgcgt tcgttttgga
    18721 caaaccggcc gtctgaacct ttgccgaaca cgcgggcgat ttcaaacacg cgcacgcggt
    18781 tttgtttgcg gttcaggttg ttttgcaaaa tttccaccaa gccgccgatg agtgtggaac
    18841 gcatcacggc atattgcgcc gccagcgggt tttgcaggcg gatggggtcg gcgttggcgg
    18901 caaaatcttg ttcccactgc tcgtcaacga aggcatagct gaccacttcg cggtagccac
    18961 gcgccgccat ttcgttgtaa acggcaaaac gcgagcgacg tgtttcgggc agttccagca
    19021 ttttcagacg gcctgacgtg taatcgtcgg ggatgttttc atagccgtaa acgcgtccga
    19081 tttcttcaat caaatcagcc tcaatttcga tgtcaaaacg gaagctcggc gcggtaacgc
    19141 ggaagccttc cgccgttttc tcgggctgca gacccaagtg ttgcaaaatg gtttccacct
    19201 gttcggcagg aatgtccacg cccaacacgg ttttcagacg gtccaaacgc aatccaacct
    19261 gcttcgcttc aggcaattcg ccttgcgctt ccaccatctc gcctgccgca ccaccgcaaa
    19321 tctgcaacac caattcggta gcacgttcaa tggcatccgc ctgcaaacgg taatccacgc
    19381 cgcgctcgaa gcggaacgac gaatccgaac cgaaaccgta ttggcgcgat ttgccggcga
    19441 tgatttcggg cgcaaaccaa gccgcttcca gcacgatatt ttgcgtgccg tctgaaaccg
    19501 cgctcgccgc gccgcccatt aagcccgcca aactcaacgc gcctttttcg tctgccacga
    19561 ccagcgtatt ttcagacagg gaaacggttt tctcgttcag gcattccagc gtttcccctt
    19621 cgcgcgcgcg gcggatgtgc aggctgccgg aaagtttgtc ggcatcgaaa acgtgcatcg
    19681 gctggccgat ttccagcatt acataattgc cgatgtccac cagcacggaa atactgcgga
    19741 tgccgctgcg ctccaaacgt tgtttcatcc aatcgggagt agccgctttc gcattgacat
    19801 tttcaataac gcggctgata aaacggccgc aatcggcagg cgcgttaatc tgcacgggct
    19861 gttttcgact gcccgtgatc ggcgcggtat ggatttcggg ctgcctgaac gcgcaccccg
    19921 tcaatgcgga cacttcgcgc gcaatgccct taacactcaa gcaatcagcg cggttaggcg
    19981 taattttcaa cgtaaacagc gtatcgtcca aatccaagta ttcacggata ttgataccga
    20041 caggcgcgtc ttcaggcaaa atgtgcaggc cgttcacacc atcatcaggc aaacctagtt
    20101 cattggtcga acacagcata ccgtttgatg gcacgccgcg cattttggtc ggcttgattt
    20161 tgaaattacc cggcaaaacg gcacccggca acgaacacgg cactttgatg cccgctttca
    20221 cattcggcgc accgcacacg atctgaacca actctcccgt acccgcatca actcgggtaa
    20281 cgttcaaacg gtctgcatcc ggatgttttt caacggattt cacttcggca acaaccacgc
    20341 ccgcaaaggc aggtgcggca gtttcagcct cttccacttc caagccggac atcgttaaca
    20401 gatgttccag cttatcggcg gaaagttcgg tatcggcttg ggttttcagc catgagtagg
    20461 agaattgcat atttttccta aggattaaat ttgattggac aacttaaggg taaccgagtg
    20521 taaattacat tacacatttt tccataaagc agtctaaaaa ttcttcattg acagctttat
    20581 ttgtcaattt ccggcgaatg cacaaggaat acaactcttc aactgcggaa taaggaaaat
    20641 attgggtatc taaattttta aacatcggaa cattcgcttc tttaaaaact ttatccctat
    20701 cttcatcagg agcaacgata acccagcgcg tattagcata tggtggcaac aggtctttca
    20761 actgcttcat ccgtgcaagg ccggaacgta cacccgtact atgttccact tccatcaccg
    20821 ccggcatcag ttttccattc ctgaaccaga tgcaatcaat attcattgcc gcctgaaccg
    20881 cgccgtcaaa cgaactgatc agtttctcgc tttctatctt ttgtacgata ccatccaaat
    20941 cagcaatttt cttatcccca taactaatac cttggtcgtt tctcgcaatc cacgtcctga
    21001 aaccaagttg aaaacctatt gctgaaaggg caatctggat ttgcacgtgg cggcgtttta
    21061 catctatatc tatttctgaa tgggttgaag gaagggataa agcatcgtaa accacattgt
    21121 tggaggggat ttccgaaata acgacatctg tctcatattc cttcattacg cctttttcat
    21181 gtggcctatc gggaagccaa ataatgtgct tgtgcccttc caccacctcg cttttattca
    21241 gacccggttg tatccgctgc aatttgcacc agtaaatttc aggtgtatgg gcaagcaatg
    21301 cctccagtgc ggaacgaaag ttataacttg cgccaaacac cctgtccaca ttgacaggct
    21361 cccccgtgga caacgcatct gccaaccgcc aaattgcttg tttcgatatc gaagtttccg
    21421 tatctcccct tttaatctta atcggacctt ccggtttttc aacatggacg atttgtatgg
    21481 tatgcgtatt tgagctgttc acatagttat aattgacatt ttttttcaag ctatcaatcg
    21541 cactgaccaa attagcagcc gtcagttttt gtccgctcat agtgattctc caaataagat
    21601 tttaatgtca tcgctatccc ttttgctgcc aaaaacggta caccgttgcc tatagttttg
    21661 aacatattgc tcaatgtcat gttatcaggc agctcaaact ccttcggcaa agactgtatt
    21721 gccaatgcct ccgcagcgga aatccttctt ggcagatagg gatgaatgtg gacttcgttg
    21781 tttccgtatg cagcagtcgg agaataacgc cagcggtgta ggcgtttata ggacttcttt
    21841 ttatcatccc cttcgcttat ggtttgaaat ttttctaaac ccgctcttgg ttggaagtgt
    21901 ttgctcgcat tcgggtgtgt atccacccca ttgcgattga accaatactg aacggtcaac
    21961 tcagggtata tgtttgcagg ctgactgacc actcttccta cttcttctgt cgtaggccaa
    22021 tctttttcca aggcatcagg gaatgaaata tgagcatccc aatcaaactt ctgcaaagca
    22081 tcaacatgct gactcaaaaa acccactagg ataatccttt ccctgtcttg cggtacgccg
    22141 tattcgatgg cattgatgag tttttcagtg catacatatc caaaatcaga aagttgccgt
    22201 ttcaacgcat tgaaaaactc cctatgcttg gcagtcctat atagtccttt cacattctca
    22261 aatacaaaaa aatcaggctg gtttttacaa attaaatcca cataactttg tgataactta
    22321 ccattctccc catccttacc tttatttttc cctgcaatag aaaaatccgg acatggggga
    22381 cctccaataa atccgacaat aatgccattt tgtttttcct ttttaaccca gccagccaaa
    22441 tcttttgctt tctcagcata aagacattcg tcaatactac ccttaaaata tccgtattta
    22501 ggcttcggga tatccattct gcttcgagag tatttatatg cttccaaaaa tgggggatgg
    22561 acttcattaa cgaaggcaat atcaaatcct gacaactcga agcccaaatc caaaaagccc
    22621 gcaccagaga aaaaagaaaa aatcaaagtt ttttttcttt cctgccgtat gcttatatcc
    22681 aaattgcagc ttttatccgt caactggaag gggagattat ttttctctcg tttgaaattt
    22741 tgacaatccc tgtttgtcgc ggacggcttg tctgactttt tcggcacagc aggttgtatt
    22801 tttaacgaca tcattactcc aaaaacgtaa aaccgcccag ccctcgacct tcagacggcc
    22861 tgttacttcc atatcccttc ggatattgcg ttctatcttg gcaatccaaa attcgcggtt
    22921 gccctttatt tccgcctttc tctgttccca atccttgccg tgccaaaact cgccatcaac
    22981 aaacacggca actttatatt ttttaaatga aaaatcaggc ttcccaaaaa tgcttccgct
    23041 atttttccga tacctgagcc ccaaagccca catcgccttc gccaacgcaa gctcgggctt
    23101 cgtccccttg cttttgttgg actgcataca ttttttgcgc tgttcggggg ttaatctgtc
    23161 catttcgtga ggcctgaaac gcgtttatac gaaagcgcat tcattttacg cggcatcgtt
    23221 gcggattccg gcagttttgc accccttgca aggaaacaac aaaacccaat cgcgcaggaa
    23281 gccacctgtt tcgtcaggtt ttccctgcaa agccgcaagg ttcgcagccg gacggactca
    23341 aaattaccaa gcatcccatc cgcacaaatc ttcagaacgc atcaattcat ctgccgaaat
    23401 cccgccgaaa tcttgccgca ggctttttca gcccgtccgg ccggacaata tgcgggttgc
    23461 actccgcttg caggctgccc gcaccattat ttcgcaaact gcttcaaaaa attcaaatcg
    23521 ttatcaaaaa acaagcgcaa atcgttcacg ttgtaacgca gcatcgcgaa acggtcgaga
    23581 ccgataccga aagcgaaacc ggtgtatttt tcgggatcga tattcacatt tctcaacacg
    23641 ttgggatgca ccatgccgca gccgccgact tccagccatt tgccgttttc gcccataatg
    23701 tcgatttcgc cagacggttc ggtgaacggg aagaaagacg ggcggaaacg tacttgcaaa
    23761 tcatcgcgtt caaagaagcg acggataaaa tccgtgaaca ctgcttttaa gtcggcaaaa
    23821 gttacaccct cttctaccca caaaccttcc gcctgatgga acataggcga gtgcgtggca
    23881 tcgctgtcca cacggtaaac gcggccgggg gcgataatgc ggatgggcgg ctctttttta
    23941 tcgagcatat agcggatttg aatcggggaa gtgtgcgtac gcaaaacatc accgttttca
    24001 acgtaaaacg tatcctgcat cgcacgggca ggatggtttg cagggatgtt cagggcttgg
    24061 aaattgtgaa aatcgtcttc gatttcaggc ccgtccgcca cttcgaaacc cattccgtga
    24121 aagagttcga ccacacgttg caaggtcagg gttacgggat gcaggctgcc gccttcctga
    24181 gcgcgtccgg gcagggtaat gtcgagggct tcggcggcaa gtcgggcttg cagcttggct
    24241 tcgttgaggg catcgcgttt ggcattaaaa gccgtctgaa accggttttt gcattcattg
    24301 atatgcgcac ctatggtttt gcgctcttca ggcgacattt gccccaaagt tttcagaagt
    24361 ccggtcaact cgccggtttt gccaagataa cgggctttga tttgttctag agcgttgaag
    24421 tcttgcgcag cttctactgc ggcaatgcct tctgcaacga tgcggtttac attttccata
    24481 atatcaagtc tgtcaattaa tgatatgcca cctgaaacaa cgaggccgcc tgtatagcct
    24541 gacatatcag actcttcaga cgaccttatt ttcttcacgg tcaatccatt ggataaattt
    24601 tctgtatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgcc gtactatccg
    24661 tactgtctgc ggcttcgtcg ctttgtcctg atttaaattt aatccactat attttaacgc
    24721 aaatttattt aaaaaaatag actttaaaga aagaaaatca atcacataga atttctgcaa
    24781 acaaagaaaa aaggaagccg cagcttcctt ttcaattttt tggattaagc agccaaagca
    24841 gatttggctt tttcaaccaa ttgtgcaaaa gcggctttat cgaacacggc caaatcagcc
    24901 aatactttgc ggtcgatttc aatagaggcg cgtttcagac cgttcataaa tttgctgtaa
    24961 gacaacccgt tttcacgcgc acctgcattg atacggacaa tccacaattg acggaattgg
    25021 cgtttgcgtt ggcggcggtc gcggtatgcg tattggccgg ctttcattac cgcctgcttg
    25081 gcaacgcggt aaacgttttt acgacgaccg cggtagcctt tggctaacgc gaagattttt
    25141 tggtgacggg cacgggcggt aacaccgcgt tttacgcgtg gcatattcta aactccttaa
    25201 gcgtagggta acattttagc aacagaagcc aaatcgcgat catttaccat agaggtaccg
    25261 cgcagttggc gtttgttttt ggtggtcttt ttagtcaaga tgtggcgttt gaacgcatga
    25321 gcgcgtttca caccgccgtt acccagtact ttaaagcgtt ttttcgcgct agacttggtt
    25381 ttcattttag gcatgggaaa actccattcg ttatcggata aggcattagg gggttttaaa
    25441 acatcggttt caaacacttg aacccacaat gccacggttt tttgccgcaa ttaaaaactt
    25501 actccgaagc ggcgatcgga gtaagcggag aattatagct ttattttttc ttcggcgcaa
    25561 tcatcatcac catttggcga ccttccattt tgggaaagga ctcgatttgc gccacttcag
    25621 ccaaatcttc ttttacacgt tccaaaagtt gcgcgccgag ttgctggtga gccatttcac
    25681 ggccgcgaaa acgcaatgtc actttgactt tatcgccgtc ggcaaggaag cggttaatgt
    25741 tgcgcatctt gatttgataa tcgccctcat ccgtacccgg acggaatttg atttccttga
    25801 tttgcacctg cttttgattt ttcttggctt cgtcgcgctt cttggcttgt tggtatttgt
    25861 atttaccgta atccatcagt ttgcacacag gcggtttagc agttggggaa atctctacca
    25921 aatcgacatc ctgcccttcg gccatagcca aagcttcacg aactgaaacg acaccaagct
    25981 gttcgcctga ctcactgatt aaacgcactt ctttggcggt aatttcgccg ttgattcgtg
    26041 cttcgcgttc ttgagcgatg atgaatactc ctataaaaat taatgattga cgagggcatc
    26101 agtgatttct tgctgcaatt gcgcaatgaa atcatccaaa tccaaagaac ccaaatcttc
    26161 tgctttgcgg cgtaccgcca ctttgttttc ctgcttttct ttatcgccga caacgatttg
    26221 ataaggaaaa cggtattggc tgttgtcgcg gattttgtaa ccgatttttt cgttacgcaa
    26281 atccaactcg gcgcggaatc ctgccgcctg caatttggca gccacttcac ggcagtaatc
    26341 cgcttgattt tcggtgatat tcataatcac catttgaacc ggcgccagcc ataacgggaa
    26401 agagcctgca tggttctcaa tcagaatgcc gataaaccgc tccaaagaac ccaaaatggc
    26461 gcggtgcaac attacagggc gagcgcggtc attgttttcg gttacatatt ctgcatccaa
    26521 gcgttccggt aagacgaaat ctagttgcaa tgtaccgcat tgccaagaac gacccaaggc
    26581 atctttgacg tgatattcga ttttaggccc gtaaaacgca ccttcgccag gcaactcttc
    26641 ccactctacg ccacaagcgg tcaatgcatc acgcaagcct tgttcggcct tatcccaaac
    26701 ttcgtcagaa cctgcacgtt gttcagggcg gagggacaac ttcacagcaa catcatggaa
    26761 accgaactgt ttgtagatgc gaaccaacaa ttcattgaat gcgcgcgctt cgctgacgat
    26821 ttggtcttcg gtacagaaaa tatgcgcatc atcttgcaca aaaccgcgaa cacgcatcag
    26881 accatgcaat gcaccgcttg gttcattgcg gtggcaagaa ccgaactcgg ccaaacgcat
    26941 cggcaaatcg cgatatgagc gcaaaccgtt attaaaaatt tgcacatgac ccggacagtt
    27001 catcggtttc accgcgtatt cgcgtttttc tgaactggtc acgaacatat tgtctttgta
    27061 gttgtcccaa tggccggatt tttcccaaaa ggttttatcc ataatttgag gcgttttgac
    27121 ctctttataa ccggcggcgt tcagctcttt acgcatatgc tgttcaatca cttgccacaa
    27181 agcccagcct ttaggatgcc aaaacaccat gcccggcgct tcgtcttgca gatggaacag
    27241 atccaattgc ttgccaagtt tgcggtggtc gcgcttttcg gcttcttcga tacgttgaat
    27301 ataggctttt aattcgtctt ttgtcgccca agccgtaccg tatatgcgtt gcagcatttc
    27361 attattgctg tcgccgcgcc agtatgcgcc cgccaacttg gtcagtttga agtttttcag
    27421 gaaacgggta ttcggaacgt gcggtccgcg gcacatatcg acatattcct gatgatgata
    27481 catccccatc gcttccactt cgggcatatc gtcaatcagg cgcagtttgt attcttcgcc
    27541 gcgctcttga aaaattttaa tcgcctccgc acgcggagtc atgattttga ccacatcata
    27601 gtcttgggca atcaattctt tcatacgcgc ttcaatggcg gcaacatctt ccggtgtaaa
    27661 cggtttttcc gtggcgatgt cgtaataaaa gccctcttca atgacggggc cgataaccat
    27721 ttttgcatta ggatagagtt gcttgacggc atgcccgaca agatgggcgc aggaatggcg
    27781 gatgatttcg atgccttcct gatctttcgg agtaatgatt tgaacagcag aattttcaac
    27841 aatcgggtcg cacgcatcga ccaatttgcc gtttaccctg cctgccaccg ccgccctcgc
    27901 caaaccgaca ccgatagacg cagcaatttg agccacggta acgggggatt cgtattggcg
    27961 gactgagccg tccggcaagg taatgttcaa catcaaacgc tccgaaagat taaaaaataa
    28021 cggcataaat gccgttatgg gaatttggta ggcacgattg gattcgaacc aacgaccccc
    28081 accatgtcaa ggtggtgctc taaccaactg agctacgtgc cttcaaagaa tttttgcatt
    28141 ttatcgggtc gttttaattt ttgcaagagg tatgtgcatt tctttatgca acattgtggc
    28201 aagatttggg gatgtcgtct gaaaaaccgt gggaattcat ttataatgcc gtcttttaaa
    28261 acatatttcg acccctgtcg cccaactgac attcaatctt atgcttaaat ttaccttaca
    28321 caaaaaagac ggatacgccc gacgcggcac gctggagttg aaccacggca aaatcgaaac
    28381 gccggtattt atgcctgtcg gtacttacgg ctcggttaag gcgatgaacc cgcaaaacct
    28441 gcacgacatc aaggcgcaaa ttattttggg caacacttat catttgtggc tgcgtccggg
    28501 cttggaggtc gtcgaacagt ttggagggct tcacggcttt atcggctggg acaaaccgat
    28561 tctgaccgac tcgggcggtt ttcaggtttt ttctttgtcg gatatgcgca agctgacgga
    28621 agaaggctgt acgttcaaaa gcccgatcaa cggcgacaag ctgtttttat cgcctgaaat
    28681 ttccatgaaa atccaaacgg ttttgaactc ggacatcgcg atgcagctgg acgaatgcac
    28741 gccgggcgaa gcaacgcgcg aacaggcgaa aaaatcgctg caaatgagcc tgcgctgggc
    28801 ggaacggagc aaaaaagcct ttgaagattt gaaaaacccg aacgcgctgt tcggcatcgt
    28861 gcaaggcgcg atgtatgagg atttgcgcga agaatcgctg cgcggtttgg aacagtttga
    28921 ttttcccggc cttgccgtcg gcggtttgtc cgtcggcgaa cccaagcccg aaatgtaccg
    28981 tatgctgcgc gccgtcggtc cgattctgcc ggaacacaag ccgcattacc tgatgggcgt
    29041 aggcacgccg gaagacctcg tgtacggcgt ggcacacggc atcgatatgt tcgactgcgt
    29101 gatgcccacc cgcaatgcac gcaacggctg gctgtttacc cgtttcggcg atttgaaaat
    29161 caaaaacgcc aaacacaagc tcgacaagcg tccgatagac gaaagctgca cctgctacgc
    29221 ctgccaaaac ttcagccgcg cctacctgca ccatctgcac cgcaccggcg aaatcttggg
    29281 cgcacagttg aacaccatcc acaatctgca tttctaccaa gtcattatgg cggaaatgcg
    29341 cgaagccgtc gaacaaggca aatttgccga ctggcaggcg cgattccacg aaaaccgcgc
    29401 acgcggcaca gactgattcc ctgcaatgcc gtctgaagcc gcttcagacg gcattttgcc
    29461 gctttaccct acccgcacta tttttcagaa accgatcatg cttccattct ttccagaacc
    29521 ctccctttcc tatacgcaac aaaaccgtac cgccgttttg ctgttgaacc tcggcacgcc
    29581 cgacgcgccg accgcgcagg cggtacgacc ttatctgaag tcctttctga ccgaccggcg
    29641 cgtcgtcgaa ctgcccaaat ggctgtggta tcccattctg catggtctgg tactgacgct
    29701 ccgccccaaa aaaagcgcgc acgcctatga aaaaatctgg tttaaggaag gttcgccctt
    29761 agaagtttac acagcacgcc aagctgccgc gcttgccaaa cgtatgcccg acctcatcgt
    29821 ccgccacgcc atgacctacg gcaatccttc ggtggcagac gtattgtcgg aactgaaggc
    29881 gcaaggcgcg ggcagactgc tggtcatccc gatgtacccg caatatgccg cttccagcag
    29941 cggagcggca gtagataagg tgtgcgaaca attactgctg caacggaacc aaatgagcgt
    30001 ccgcaccgtt tcgcgctttt atgacgatac cggctacatt gatgcgatga aaaaccacat
    30061 cctccgatat tgggcggaac acgggcgcgg gaaaaaactg atgttgagtt ttcacggcgt
    30121 accgcagaag caccacgacc tcggcgaccc ctatcccgac gagtgccgcc acaccgccaa
    30181 actgcttgcc gaagcactgg aactgaccga agaccaatat gtcgtatcgt tccaaagcca
    30241 attcggcagg gcaaaatggg ttacaccgag cacgcaggat ttgttcggca aactgcccaa
    30301 acagggggta accgagctgg acgtattttg cccgggcttt ttggcagact gtttggaaac
    30361 catggaagaa atcgccctga tggggcggga acagttttac gaagcaggcg gcaaaagcta
    30421 ccgctacatc ccctgcctca acgacaaccc cgactggata gacgcactcg tcgcacttgc
    30481 cgaagaaaac cttggcggct ggcgttgaaa ctgtttgacc catgtctaaa atgccgtctg
    30541 aacgccgtgt tcagacggca ttttcccgct actttagcgt atgatggcat ctatgccgga
    30601 caacaacatc aagtgcaacc ttactaccga ttcggcaaaa aaatctttca cccgctacgc
    30661 aaaatcaaca acaaggtttc aaacattaag gaaacaccaa tgaacacaac ccgactgccg
    30721 accgccctcg tcttgggctg cctctgcgcc gccgcttctg ccgccgacaa cagcatcatg
    30781 acaaaagggc aaaaagtgta cgaatccaac tgcgtcgcct gccacggcaa aaagggcgaa
    30841 ggccgcggaa ccatgtttcc gccgctctac cgctccgact tcatcatgaa aaaaccgcag
    30901 gtgctgctgc acagcatggt caaaggcatc aacggtacaa tcaaagtcaa cggcaaaacc
    30961 tacaacggat tcatgcccgc aaccgccatc agcgatgcgg acattgccgc cgtcgccact
    31021 tatatcatga acgcctttga caacggcggc ggaagcgtta ccgaaaaaga cgtaaaacag
    31081 gcaaaaggca aaaaaaacta aacagacaaa atgccgtctg aaaccggcaa tccggcttca
    31141 gacggcattc aaatcaaacc tttctaacgc ggtactttca gccccataga cccgatattg
    31201 ccgtccccgt caacctcctt cgcggtcagc ataaagccgc cgatgtcaat acggccgcac
    31261 tcgaccggct cctgactgtc ggaacgctta tcgaaaagct cggcaaggct caaaccctct
    31321 tcgcccgctt ccagcctcaa accgtaagca agtgccaaat cacccgaacg tgctgccggc
    31381 aaaacgacaa actcaccgaa gaaatcaaaa tactcacgga cgctaatacc cgtctcggta
    31441 aagtatttcg ccattttata gtgaa
//
LOCUS       DAWEWP010000005        28554 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWEWP010000005 DAWEWP010000000
VERSION     DAWEWP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28554
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.005"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(287..4456)
                     /locus_tag="WGN53_000139"
     CDS             complement(287..4456)
                     /locus_tag="WGN53_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ0139185.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTLWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNINRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGRRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
     gene            complement(4511..6358)
                     /locus_tag="WGN53_000140"
     CDS             complement(4511..6358)
                     /locus_tag="WGN53_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ0139186.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLHSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6770..7999
                     /gene="sstT"
                     /locus_tag="WGN53_000141"
     CDS             6770..7999
                     /gene="sstT"
                     /locus_tag="WGN53_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ0139187.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAYTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8111..9604
                     /locus_tag="WGN53_000142"
     CDS             8111..9604
                     /locus_tag="WGN53_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0139188.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTE
                     DVGEEVLPKEKKDEEAVSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPEN
                     KDEGPQNDMPQNAADTDSSTPNHPPAPNMPTRDMGNQAPDAGESAQPANQPDMANAAD
                     GMQGDDPSAGENAGNTADQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANG
                     IKLDSGSENVTLTHCKDKVCDRDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVG
                     LVADRVEKNGTNKYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVD
                     GEAVSLTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGE
                     VLHFHMENGRPSPSGGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGT
                     WTENGGGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9680..9895)
                     /locus_tag="WGN53_000143"
     CDS             complement(9680..9895)
                     /locus_tag="WGN53_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139189.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9888..10103)
                     /locus_tag="WGN53_000144"
     CDS             complement(9888..10103)
                     /locus_tag="WGN53_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ0139190.1"
                     /translation="MKEYKVVIYQESQLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10178..11521)
                     /gene="argG"
                     /locus_tag="WGN53_000145"
     CDS             complement(10178..11521)
                     /gene="argG"
                     /locus_tag="WGN53_000145"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ0139191.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKK"
     gene            11646..12446
                     /locus_tag="WGN53_000146"
     CDS             11646..12446
                     /locus_tag="WGN53_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ0139192.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALELPVVRHPEAAGFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYAGRFNQTQRGSRSVYVFEQPESRITPIMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12486..13472
                     /locus_tag="WGN53_000147"
     CDS             12486..13472
                     /locus_tag="WGN53_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ0139193.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDMMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFQKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPDIPVYLVAEDMCASG
                     CYYIAAAADKIYADSSSIVGSIGVIGSSFDATELMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKAGVREALQAVR"
     gene            14073..14363
                     /locus_tag="WGN53_000148"
                     /pseudo
     CDS             14073..14363
                     /locus_tag="WGN53_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14430..14804)
                     /locus_tag="WGN53_000149"
                     /pseudo
     CDS             complement(14430..14804)
                     /locus_tag="WGN53_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14977..15333)
                     /locus_tag="WGN53_000150"
     CDS             complement(14977..15333)
                     /locus_tag="WGN53_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ0139194.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(15395..15511)
                     /locus_tag="WGN53_000151"
                     /pseudo
     CDS             complement(15395..15511)
                     /locus_tag="WGN53_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(15598..15810)
                     /locus_tag="WGN53_000152"
     CDS             complement(15598..15810)
                     /locus_tag="WGN53_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139195.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(15886..16245)
                     /locus_tag="WGN53_000153"
     CDS             complement(15886..16245)
                     /locus_tag="WGN53_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0139196.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(16242..16460)
                     /locus_tag="WGN53_000154"
     CDS             complement(16242..16460)
                     /locus_tag="WGN53_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139197.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNIL"
     gene            complement(16481..16687)
                     /locus_tag="WGN53_000155"
     CDS             complement(16481..16687)
                     /locus_tag="WGN53_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139198.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKDLPIR"
     gene            complement(16653..16853)
                     /locus_tag="WGN53_000156"
     CDS             complement(16653..16853)
                     /locus_tag="WGN53_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139199.1"
                     /translation="MASDLLSKQTSDKILGYQTEHRRVRYDINNNIYVLANPKTFKIK
                     TMFKPNLGKKYYDGEFKKDMGN"
     gene            complement(17213..17557)
                     /locus_tag="WGN53_000157"
     CDS             complement(17213..17557)
                     /locus_tag="WGN53_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139200.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(17595..17918)
                     /locus_tag="WGN53_000158"
     CDS             complement(17595..17918)
                     /locus_tag="WGN53_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139201.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(17921..18151)
                     /locus_tag="WGN53_000159"
     CDS             complement(17921..18151)
                     /locus_tag="WGN53_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ0139202.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENTRTGEKTDFKFIENTRTGEKTDFKFKD"
     gene            complement(18221..19336)
                     /gene="mafB"
                     /locus_tag="WGN53_000160"
                     /pseudo
     CDS             complement(18221..19336)
                     /gene="mafB"
                     /locus_tag="WGN53_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(19376..19501)
                     /locus_tag="WGN53_000161"
                     /pseudo
     CDS             complement(19376..19501)
                     /locus_tag="WGN53_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            complement(19587..19958)
                     /locus_tag="WGN53_000162"
     CDS             complement(19587..19958)
                     /locus_tag="WGN53_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0139203.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(19955..20206)
                     /locus_tag="WGN53_000163"
     CDS             complement(19955..20206)
                     /locus_tag="WGN53_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139204.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(20338..20544)
                     /locus_tag="WGN53_000164"
     CDS             complement(20338..20544)
                     /locus_tag="WGN53_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139205.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(20554..21222)
                     /gene="mafB"
                     /locus_tag="WGN53_000165"
                     /pseudo
     CDS             complement(20554..21222)
                     /gene="mafB"
                     /locus_tag="WGN53_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(21292..21609)
                     /locus_tag="WGN53_000166"
     CDS             complement(21292..21609)
                     /locus_tag="WGN53_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139206.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(21606..22988)
                     /gene="mafB"
                     /locus_tag="WGN53_000167"
     CDS             complement(21606..22988)
                     /gene="mafB"
                     /locus_tag="WGN53_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0139207.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDNHDSRSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDVRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDGINSAKQWADAHPNITATAQTALSVAEAAGTVWGGKKV
                     ELNPTKWDWVKNTDYKTPAARPMQTLDGEMAGGNKPVVKSIRPTTRDELRQALQEQGF
                     RRTGSDAAQYETWKGPDGVKIDIRPNGEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPN
                     NHHHSGYFVK"
     gene            complement(23028..23990)
                     /gene="mafA"
                     /locus_tag="WGN53_000168"
     CDS             complement(23028..23990)
                     /gene="mafA"
                     /locus_tag="WGN53_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0139208.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(24174..24893)
                     /locus_tag="WGN53_000169"
     CDS             complement(24174..24893)
                     /locus_tag="WGN53_000169"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ0139209.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(25110..25964)
                     /locus_tag="WGN53_000170"
     CDS             complement(25110..25964)
                     /locus_tag="WGN53_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ0139210.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDVGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(26093..26821)
                     /gene="rpsB"
                     /locus_tag="WGN53_000171"
     CDS             complement(26093..26821)
                     /gene="rpsB"
                     /locus_tag="WGN53_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ0139211.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(27022..27231)
                     /locus_tag="WGN53_000172"
     CDS             complement(27022..27231)
                     /locus_tag="WGN53_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139212.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARTLIYLVFWAVLPTVFTVFV
                     VTIRRRNRLMKQQEQAEFEQQRAQR"
     gene            27309..27890
                     /locus_tag="WGN53_000173"
     CDS             27309..27890
                     /locus_tag="WGN53_000173"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ0139213.1"
                     /translation="MRNEEKHALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            27951..28541
                     /locus_tag="WGN53_000174"
     CDS             27951..28541
                     /locus_tag="WGN53_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0139214.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHEMGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         6677 a         6668 c         7333 g         7876 t
ORIGIN      
        1 acctgtgtat cgctgtaaat cttagagatg gcggaatata gcggattaac aaaaaccggt
       61 acggcgttgc ctcgccttgg ctcaaagaga acgattctct aaggtgctca agcaccgagt
      121 gaatcggttc cgtactattt gtactgtctg cggcttcgtc gccttatcct gatttttgtt
      181 aattcactat atcgattctg ttcccttgca ggcggacggc ggcggcggac ggtggaaaag
      241 caggttttca aatgtccggt ttggcggcgc gccgtctgaa aaccgcttat tttcctttgc
      301 cgtttccggc ggagtctttt ttgtccgaac cggagaagcg gtcgaaacgt atggtgtatg
      361 tcagctcgcc gcccgacgaa cgtctgccga tacgggcaac cgcctgtatg gcgcgggtca
      421 gccggtaaat cagtttgacg gactgttccg cgctggagat gctgtattcg tagccgatgt
      481 agagtttgcc ggtcagttgt ttgccgacgg tcagcacctg ttcggcgggg ttgagttcgc
      541 cggtttgcgc gttgcggctg cgcttgctgg taaagcccaa atcatccacc agcccgatgc
      601 ggtcgttgat ttgcccggca agcagcgcgc ctgcggctgc ggacagggcg gcattgtcgc
      661 cgctgctgcc actgccggcg cggttgagga tgagccagga gagcttgtct ttttcactca
      721 tcggttcgtt tgccgtcagc gtaatgcgcg gactgttgag gctgcccaat atttccacgc
      781 ccgcaccgac gggggaaagg cggcgttcgg cgcggatgtt gaggttgggg tcgttgagcg
      841 ggccgacaaa ggagaccgtg cctttggtaa tgtccaaatc ctgcccgtat gccttataac
      901 gccctttgat gacgcggacc gtgcccacgc cccgcacgct tccgcccgat tgggcggtca
      961 gggtcagttt gccgcctatg gtaacgtccg cgccgtagcc ggcgaagcgg atgccgtcat
     1021 tgaggtctaa agtcaggttc atattgacgg ggagcggtgc cgccgcctct tttttaactt
     1081 cgcctaatac gacgacatcg tcgccgacgg acggcatcga ggatttttgc gaaccgaaca
     1141 gcccctgatc cgttttaatc atcccggtaa cggatatgcc tttttgcggc gaatagcgca
     1201 ggcgggtgtt gccggaaacc gtcaggcggc ggttagggcg ggacaggatg cggtatttgt
     1261 cgaacaccgc gccgatatcg acatcgggtc cgctgttttc cataccgacc gtgccggaga
     1321 gttccgccgt tccttcgtgc cggaatttca ggctgtcgat tacccatttt ctgcccgcga
     1381 tatgcgaacg cagcgagccg ttgtccaaga tgatgccttg ggtttggttg cggtaataga
     1441 gcttgtcgcc gttgatgctg ccgcccaagt gcgggtcggc gatgctgccg ccgagggtta
     1501 cggcggcatt caggctgcct ttgacggttt gtccgacggg caggaagttg cggaatactt
     1561 cggcatcggc aacggtcagg ttgagcctgc cgcccaaagg cgcggtatcg aaagagcggc
     1621 tttgcccgac ggtgatgttg ccgttgattt tcccgtagtt gctgctgccg ttgacggcgg
     1681 cattgacgga cggagagccg acccgtccgc cgatttgcgc ggcggcattc aggctgccgg
     1741 taatgttttg cgcggcggcg ggcagaaagg gcttcaatgt gcccaagtcg ggaagggagg
     1801 cggtaatcct gccaccgagc ggtgcatttg ccatattgcc gccgaaggcg ttgccgatgt
     1861 ccaaatcggc gttaatccgc ccgaaacgtg cgccgccgtc aagcaggatt ccgatacggt
     1921 cgttttgaaa gcgcgttttc agggaaaatg cgttcaaacc caaagcctgc ccgccgggca
     1981 atacggcatc gccgctttgc cgattgatat tgaggtagcc gcgcgcgttg cgcccgtagg
     2041 cgacatccca gtcgccgttt aaaaccagat tgtgttcgaa gggcggtttg aagaaattgt
     2101 gcaactcggc gatatgcaga cctcgtgcgc cgccttttgc cgatatgccg gttttcctgt
     2161 cccaggaaaa gtgttgcagg ttgaggctgc cgcccatagc ctgccaattt gccgcacttg
     2221 ccgccacacg ttccgcaccg gcttcgagcg tcatacggtt ttgcagtttt aggttgaatg
     2281 cgccgccgat gtcgaggatg ccgatgctgc ccttccatag ggtaagttcc ctgttgatgc
     2341 cgcctgaagc gtccaaatcg aatttgaacg gtttgccatc cagcgtcatg gcggcgtgtg
     2401 tgcggatgcg gtgctgcacg cccgtgccgt ccagcatcag gtcggcggta tcgacaaccg
     2461 ccgctccgcc cgacagcgaa aggcggctgc ctttgatgtc ggcgcgtatc gggcggcttg
     2521 tgtcgggcga acctttgagc gtgaaatcga gcgaacggat gtctgccgcc ttgccgatgt
     2581 gcaggttgcg cgccgcgccg gaaaggtcgg tttcaaaggt tcggatgccg ccgtccaaat
     2641 cgccggaaag gtgtccgcgt acatttaaag accccgcgag tccgaaaccg aaacgggata
     2701 aatcgggtgc ggtgatgttg aggttgagcc ggtcgccttt tttgccgaag ccgccgtctg
     2761 ttttaataat gttccgcccc agccgcaaat cgacggcggc acgcggaagg tggcgggact
     2821 cgtaaacaat gtcggcactg ccggcaatcg gtacgccgtt gaacgtgccg ggtaaaaacc
     2881 gcattttgcc tgtgaatttc tcttttgcca gttcgccggc aaggtttatt gagccgttga
     2941 tattgcctgc cggaagttgc ggattgatgc gcgaagggtc gaatgcgcgg gaacggatgt
     3001 ccagcttgag caggcggtct ttaaacagct cgagatagcc ttgcgcggtc aggctgcctt
     3061 gcccggcggc gatgttgacg gtgtcgagca ccagtttccg ctgtccgttt gcggggtcgc
     3121 ttgcaatggc gaggctgccg tccgtgcgcg ccgtgccgat gccgagttgc caagagattt
     3181 tgggcgaggc ggtcgtgcca ccgatgccga tgctgccgtc caacctgcct ttgaacgcgg
     3241 tttgcagtac gtcttccgcg ccgacggagt tgatgcctat atttaaatcg aggatgtctt
     3301 tttcggtgtc gattttgccc gacagcctga tgccgccccg tccgagcagg gcggcggacg
     3361 tattgccgat atgtaccgtg ccgtcctgcc ggatgacaaa gccgcctaaa acctgacgga
     3421 cggggatgcc gttgcggtcg gcaaagccgg ctttggtgtt ttccaaatcg agcgaacctt
     3481 ccagcgcgat gccgtctgaa aacgacggga tggcggtcag gtcgaaattc agcccggcat
     3541 cgggcaggga gggcacgaag gcggccgggt tgatgttgaa ccctttgacc agcacttctt
     3601 ccaatgtttt atccaatgat tcggcaaacg ggtggatgac ggattttccc gagaggcgga
     3661 tattgccgcc gtcgatcgcc agttcggcgc gcacatcctt caggctgccg ctcagccgag
     3721 ccgtactgtg tatggttttg ccttcgagtc cgcctttggt gtaaatggcg gtatcgaggg
     3781 caaacggttt tttcaagccg accgaggctg accccgacga actgctccac ggcgtgtcgg
     3841 cagccttcag gtcgaggcgg tgtcctttgc ggtcgtaacg gtatgaagca tccagccgtt
     3901 cgagatagac ggtttgtttg tcaaaggctt tgcccatgct gattttgccc gtctcgaagc
     3961 ggtcgagata gacggcggca ggcaggtcta tgctgtcggg aaggctgagc ggcgggcgtt
     4021 cttctttagg cggagtcggt ttggtaacga tggcgatgtc gccggcggaa atttcggtaa
     4081 tgtgcaggct gcggcgcatc agttcggacg gtttccacgc gaagcggaag cggctgattt
     4141 taaggtctgc cccctcggtt tctatcgacc agttgtcgcc gtcgaagccg tcgagcagcg
     4201 tgcctttgag gttttgggag gaaatgttta cgccgaacca agacgggatt tggtacagcc
     4261 cgaagcgcaa acccgcttcc gtgccggcga gccagccgag gaaacatact gccaaaatca
     4321 gaacagacag cagtgccgcc gacagcttca gcagcgggcg gcgttttttt gccggcgggg
     4381 tagggcggtg ttcagacggc attttgcgcg tgccgttttc ggtcggatcg gtatctgtcg
     4441 gtgcggtatc ggtcataatg tctgtttcaa aatggtttgc tgcaatgccg tctgaagcgg
     4501 ccgtatcggt ttagaagcgc gttcccaagc tgatgtgcca gcggattttc ttgtcgctgt
     4561 gcccgtaggc gatgtcgaag gaaaacggcg cgagcgggct gaaccagcgc acgcccagtc
     4621 ccgaaccgtg tttcagcttc atacgtttga aattggcggc ggcgtcgccc atatcgtgga
     4681 acaccgcgcc ggaaagggtg cgcgtaaacg gcagttggta ttccaggctg cccaccaaga
     4741 gggcgcgttc gggcaggacc gatccgttcg ggccggcaag cccgatgctg tcgagttcgt
     4801 aaccgcgcac ggaagacgcg ccgccgctgc ggaacatcag ccctgaagga acgttggcat
     4861 tgtcgcgggc aacggtgtaa cccgcttgtc cgcgtatgat gaacgtgccg agttttttgt
     4921 tttcgggcgt gaagaaataa cctgcgcggg cagaggtgcg gatcagcgcg gtggaggaca
     4981 ggaatgcgcc caaagtcgta ccgattttgc cgtcgaggta atggccgttt tcgggatgca
     5041 gcacgttgtt gagcagctgg cgtttccaag aggcggtcag catcgtggcg tggctgttgc
     5101 ccaaatcgat atccgagccg gggattttcc ggccttctgc gagaaactcc gcccccagcc
     5161 tggcatcgat gcccgcgcgg tcgcgcacat accagatgcc gccggagaag gcgcgttttt
     5221 cgaggttttg ggtggtcgaa cggttgtagg aaacgttgct tgtccagtag ttgccccgat
     5281 agttgcgcgg ctggctgatg ccggcggcaa gcgtggtttc gtatttgtcc atatcccaga
     5341 cgaccgaacc gatatagcct ttgttgaaga ggttgtaata gtcgtaggcg attttgccgc
     5401 ccaaaccgta ttccgaatcg aggcggatgc cggtttcgag cttgtggcgt ttgacctcgg
     5461 ttacgctgac tttgacgggg acgcggtcgc cttggaggcg gtcgaagtcg gcttgtacgg
     5521 acgcgccgga ataatgcccg ttttgttcga gcgcctgttg gaagtcgagc agcaggtcga
     5581 ggtcgtaggg cgtgcccggt tggaagcgcg ccaagccgga gacgatttgt tcggggtaac
     5641 gctgcgtgcc ggtaatttca aagtcgccga aggcgatggg gcggccgctg tccacgacga
     5701 cgttcaaatc ggcggtggcg gtatcggggt tgacggccgc ccgggtgttg ccgagcttgg
     5761 caagcgggta ggctttgcgc gttaccgcgc cgaggacgga agttttgctg ttttcccaac
     5821 tgtcctgatc gaaatcactg cctaccggct gctgccagtt ttccagcgcg ttgcggtagt
     5881 attcggcgag gttgccgtct gaaaggatgt cgccgaggat ggcgacgccg acgttggcga
     5941 ttttggtgcg cgggcccggt gtgatgtgta ccgtataagc tccgtctttt tccgtcaggc
     6001 tgactttgct gctgaaatag cctttgctgt ggagcattgt tttaacgttg tccggtgctt
     6061 cttcggcgag gaagcccgtc tgttccttgt ccaatacttc ttcctgctgc tgcgtgatga
     6121 gcggcaggtg ttcttcgacc atatctttga tttcactatc ctgcgtgtcg atgcggacgg
     6181 ggaatttggg ttttaattta actgattcgg tgtcggggct tttgtttttg aacaatgcga
     6241 aacccgccgc cttgttttcg gaaaggtcgg cggcaggcgc gtatgcgtgc ggaaagaaaa
     6301 ataaagccgg caggagcagg gcggtcggtt taatcatcat ggtacgggtg tcgtgcatcg
     6361 gtgtggcggt tttcgattct aacactgttc ggacagggtt tcgaacggcc gtttttgtgt
     6421 tgttcgtacg gggcgggacg cttcagacgg catactgccg ccggtatttc ggaggttgcg
     6481 gcccgccgga aatgtatctg cttgttttaa ggtattttgt ttctaatccg catatggaaa
     6541 acaggctgcg tttcggaata gtgccggaaa ataggcagga ttaaataaat ttattttcgt
     6601 tcaataaatt aaaccgttgc gccgaatgat aaagcaaagc ctgtccggca ctgcataggg
     6661 caaatcaacg caaccttaaa acacagccga aaaataaaaa aaaacacaga gaatgagttt
     6721 tcttttaaaa tacaacattt tttaacatat cacaacataa ggaagaatta tggcgttcgg
     6781 caaatcctta tttcatgcga tcggccgcgt cagcctggtc aggcagattg ccgccggttt
     6841 ggcgttgggc atcgtaatcg gttcggtttc cccgcaactg ggcttggcgg caggcttgtt
     6901 cggcagcctg tttgtcggcg cgctcaaagc ggtcgcgccg gttttggtat ttattttggt
     6961 ggcggccaca atcgcgcagc accaaaaagg caacaaggcg catatcaggc cgattatcgt
     7021 cctttacctc atcggtacgt tttccgcagc cctgaccgcc gtcatcgccg gtatggtttt
     7081 cccgacgcac attgttttgg cgggcgcggg cgatgtgtcc gccgcgccgc cttccggcat
     7141 tgtggaagta ttgaaatcgc tgctgatgaa cctggtcgcc aacccgatta acgcgattgc
     7201 caatgccaac tatatcggca ttttggcttg ggctttggtt ttgggcgcgg cgttgcggaa
     7261 tcacggttcg gacgttacgc ggcaggtcgt tgccgatttg gcggaagcgg tttccaccgt
     7321 cgtgaaatgg attatccgtt ttgccccttt gggcattttc gggctggtgt cgtccacaat
     7381 cgcggagacg ggtttcggcg cgctggcggg ttatgcgaag ctgcttgcgg tactgttggg
     7441 ctgtatggcg tttatcgcgc tggtggtcaa tcccgccatc gtgtggtgga aaatccgccg
     7501 caacccttat ccgctggtgt ttacctgcct gcgcgaaagc ggcgtgtatg ccttctttac
     7561 ccgttcttcc gccgccaata tccccgtgaa tatggctttg gcgaaaaaac tggggctgca
     7621 cgaagacact tattcgattt ccatcccgtt gggggcgacc gtcaatatgg gcggcgcggc
     7681 gattacgatt accgttttgg ctatggcggc ggcgtacacc caaggcatac aggttgattt
     7741 tgccaccgcg ctgctgttga gcctggtggc gacggtcagc gcgtgcggcg cgtccggtgt
     7801 ggcgggcggt tcgctgctgc tgattccgct ggcgtgcagc ctgttcggca tcagcaacga
     7861 tgtcgctatg caggtggtcg ccgtcggctt tatcatcggc gtgattcagg attcggcgga
     7921 aacggcgttg aattcttcaa ccgatgtttt gtttaccgcc gccgccgatt tgggcagaca
     7981 gagaaaccgg gcggaataaa ccaagctatg ccgtctgaag cccttttggc gttcagacgg
     8041 catattttta caattttatg ggcatatggc ggaaacaata ccggctgcct tgaatatgaa
     8101 ggagatgatg atgtttaaac gcagtgtgat tgcaatggct tgtattgttg ccctttcagc
     8161 ctgtgggggc ggcggtggcg gatcgcccga tgttaagtcg gcggacacgc tgtcaaaacc
     8221 tgccgcccct gttgttactg aagatgtcgg ggaagaggtg ctgccgaaag aaaagaaaga
     8281 tgaggaggcg gtgagtggtg cgccgcaagc cgatacgcag gacgcaaccg ccggaaaagg
     8341 cggtcaagat atggcggcag tttcggcaga aaatacaggc aatggcggtg cggcaacaac
     8401 ggataatccc gaaaataaag acgagggacc gcaaaatgat atgccgcaaa atgccgccga
     8461 tacagatagt tcgacaccga atcacccccc tgcaccgaat atgccaacca gagatatggg
     8521 aaaccaagca ccggatgccg gggaatcggc acaaccggca aaccaaccgg atatggcaaa
     8581 tgcggcggac ggaatgcagg gggacgatcc gtcggcaggg gaaaatgccg gcaatacggc
     8641 agatcaagct gcaaatcaag ctgaaaacaa tcaagtcggc ggctctcaaa atcctgcctc
     8701 ttcaaccaat cctaacgcca cgaatggcgg cagcgatttt ggaaggataa atgtagctaa
     8761 tggcatcaag cttgacagcg gttcggaaaa tgtaacgttg acacattgta aagacaaagt
     8821 atgcgataga gatttcttag atgaagaagc accaccaaaa tcagaatttg aaaaattaag
     8881 tgatgaagaa aaaattaata aatataaaaa agacgagcaa cgagagaatt ttgtcggttt
     8941 ggttgctgac agggtagaaa agaatggaac taacaaatat gtcatcattt ataaagacaa
     9001 gtccgcttca tcttcatctg cgcgattcag gcgttctgca cggtcgaggc ggtcgcttcc
     9061 ggccgagatg ccgctgattc ccgtcaatca ggcggatacg ctgattgtcg atggggaagc
     9121 ggtcagcctg acggggcatt ccggcaatat cttcgcgccc gaagggaatt accggtatct
     9181 gacttacggg gcggaaaaat tgtccggcgg atcgtatgcc ctcagtgtgc aaggcgaacc
     9241 ggcaaaaggc gaaatgcttg cgggcacggc cgtgtacaac ggcgaagtgc tgcatttcca
     9301 tatggaaaac ggccgtccgt ccccgtccgg aggcaggttt gccgcaaaag tcgatttcgg
     9361 cagcaaatct gtggacggca ttatcgacag cggcgatgat ttgcatatgg gtacgcaaaa
     9421 attcaaagcc gttatcgatg gaaacggctt taaggggact tggacggaaa atggcggcgg
     9481 ggatgtttcc ggaaggtttt acggcccggc cggcgaagaa gtggcgggaa aatacagcta
     9541 tcgcccgaca gatgcggaaa agggcggatt cggcgtgttt gccggcaaaa aagagcagga
     9601 ttgatgctga tgccgcctga agcgggggcg ttcagacggc attgtgatgt ttgggttgcg
     9661 ggtccggttc aagtccgcat cagttgataa acccgtcgtc tttcattttc ttttcggaaa
     9721 aatactggcc gaataccagc aaggcggcga ggatgatgac aaacagcccc gccatagtca
     9781 gtgtgatttt gaagaacaat tccgcttcga gaaaagaaaa ccaaagttgc acaatcagca
     9841 gcagcaccca gccggcaaac agtccgagac aggcataaac gccaagctta aacacggtcc
     9901 cgctccaaaa tgacgacgta ggcctcgcgt ttgaaaaaca acagcatacg gcgcaaatct
     9961 ttttccatcg ttacaacccg ccagccttcg ggggtttgtt tgttgaggaa cgcgctgaaa
    10021 ttgacggggt tgacctttgc cgcgccgaaa aacaggctgg acaactggct ttcctgataa
    10081 atgacgactt tgtattcttt cataggattc cttttaacat aagggatcat cttttcagac
    10141 gaccccttaa gttgctaatg atgcaaaaca gcaaacctta tttcttattg cccaactgcg
    10201 gtaataccga gccttcgccc agcgacagca aaccgctttg cgagtagatg cccagtttgg
    10261 cgcgggtgtc ggtgatgtcg aggttgcgca tcgtgagctg tccgatgcgg tcgagcgggg
    10321 taaacgccgc accttcgact ttttccatac tcaggcgttc gggttggtag gtcaggttgg
    10381 gcgattcggt gttcagaatc gagtagtcgt tgccgcgccg cagttcgagg gtaacttcgc
    10441 cggtaacggc tttggcgacc cagcgttggg cggtttcgcg caacatcaag gcttggctgt
    10501 cgaaccagcg gccttggtag agcaaacggc cgaggcgcag gccgttgatg cggtattgtt
    10561 cgatggtgtc ttcgttgtgg atgccggtca ccaagcgttc gtaggcgatg tggaacaacg
    10621 ccatacccgg ggcttcgtag atgccgcgcg atttggcttc gatgatgcgg ttttcgattt
    10681 ggtcgctcat acccaagccg tggcggccgc cgatgcggtt ggcttcgagg aagagttcga
    10741 cggggtcggc gtattctttg ccgttcagtg caaccggcac gccttcttca aagcgcacgc
    10801 tgacttcttc ggggctgact tcaacgtttt cgtcccaaaa ggcaacgccc ataatgggtt
    10861 tgacgatttt gatgcccgag ttcaaaaatt ccaagtcttt ggcttcgtgg gtcgcgccga
    10921 gcatattgga atcggtggag taggcttttt caaccgacat tttgtagttg aagccgttgg
    10981 caatcagaaa ttcgctcatt tcgtgacggc cgccgagttc gtcgataaat tgctggtcga
    11041 gccagggttt gtagattttc agcgcgggat tggtgagcaa accgtagcgg tagaaacgct
    11101 cgatgtcgtt gcctttgtag gtgctgccgt cgccccagat gtttacgtcg tcttctttca
    11161 ttgcggaaac aagcatcgtg ccggttacgg cgcggcccag aggcgtggtg ttgaaatagg
    11221 cgatgccgcc ggtggaaacg tggaacgcgc cgcattggat ggcggcgatg ccttcgtgtg
    11281 ccaactgcgc gcggcagtcg attaagcggg cgttttccgc accgtattcc atcgcttttt
    11341 tgggaatggc gttgtagtcg tcttcatcgg gctgaccgag gttggcagtg taggcataag
    11401 gcagtgcgcc tttgagtttc atccacaaca gcgcggcgga ggtatcaagt ccgccggaga
    11461 aggcgatgcc gactttctga ccgacgggga ggttttgtaa aatagtatgg ttttggctca
    11521 tgaaaaaatc ctcggtagct tgaaaaatca aagccattat cttataaata ggaaatattg
    11581 tcagccatca tgccgtctga acgggtattc cgccgtttca ggcggtattt aaaaccgaaa
    11641 acattatgaa tgcgttcaac ctcatcatca tcggcgacga aatcttacac ggcagccgcc
    11701 aagacaagca tttcgccttt ttcaaatccc tgctcgaatc caaagggctg aagctcaatc
    11761 aggtgcaata tctgcccgac gaacccgatt tgctggtcag gcaactgcgc cgcagctttt
    11821 cagacggcat accgacgttt gttaccggcg gcatcggcgc cacgcccgac gatcataccc
    11881 gccaagcggc cgccgccgct ttggagttgc ccgtcgtccg ccatcctgaa gccgccgggt
    11941 ttatcgaagg cataacccag aaacgcggcg agccgcttga ttcgccggaa cacgcccaac
    12001 gcctgaaaat ggcagatttc cccgaaggcg cggaattggt gcccaatccg tttaataata
    12061 tcgccggatt ttccattcgc gagcattatt tcttccctgg ttttcctgtg atggcgcacc
    12121 cgatggcgga atgggttttg gatacttatt acgccggccg cttcaaccaa acccaacgcg
    12181 gcagccgcag cgtgtatgtg ttcgagcagc ccgaatcgcg cattacgccg attatggagc
    12241 atatcgagca aacttatccc ggcgtgcgtt cgtacagcct gcccagcgtc ggttggacgc
    12301 attcagacgg cacgcaggtc aaaccgcaca ttgagttcgg catcaaagcc gaaggagaag
    12361 ccgtcaacct tttggacgcg gcgtgggcgg aagtgttgca cagcttggac ggattgggag
    12421 cagagctgaa aaatcgggta aactgaaaac cgtttgccca aatcggacga tatttaagga
    12481 aaaccatgca ataccgaatc agaagagaaa acgaagcacc cgaagcaaaa aatgcggggg
    12541 aaaccttatg ggagcgcgat atgatgcgcg aagtgctgtt gtccgcctat caggacaggc
    12601 gcagggagcg gatgtggaaa aacatctggc gcgccgtcag caccctgatt ctggttgccc
    12661 tgattgcagg cattttccaa aaagacgaag ccgcattgca gttggcgggc aacacgccgc
    12721 ataccgcagt cgtcaacctg tacggcgaaa tcggcaacgg tgtagaagac caggtcaaaa
    12781 aactcaaaga cggtatggag gccgcctaca aaaacccgca ggcaaaagcc atcgtcatcc
    12841 gcgccaacag ccccggcggt tcgcccgtcg tctccaacac cgcttttgaa gaaatacgcc
    12901 gtctgaaagc gcaacatccc gacattcccg tttatctcgt ggcggaggat atgtgtgcat
    12961 ccggctgcta ctatattgcg gcggcggcag acaaaattta tgcggactcg tccagcatcg
    13021 tcggcagcat cggcgttatc ggcagcagtt ttgatgctac tgaattaatg gaaaaaatcg
    13081 gcgtgaaacg cagggttaaa attgcaggca gcaacaaagg catgggcgat ccgttttcgc
    13141 ccgaaacgcc cgaacagtcg aaaatctggg aagaaatgct gaccggcata cacggcgagt
    13201 tcatcaaagc cgtcaaaacc ggacgcggcg gccgcctcaa attccgacaa tatcccgacg
    13261 tgttcagcgg tcgcgtgtac acaggcgcgg atgcgctgaa agtcgggctg gtggacggac
    13321 tcggcaatat ttacagcgtg gcgcgagacg tggtcaaagc ccccgatgtg gtggattaca
    13381 ctccgaagga cgatttcggc agaatcttgg gcagacgctt cggcgcagaa ctcaaagccg
    13441 gcgtgcgcga agccctgcag gccgtccggt agcgttgttt acagtatttc taagggaata
    13501 ccaaaataaa aatatacaca aaaacactca cctccccagt atgctgccac taagtcagcc
    13561 gcgtcactat ttaccgtgca cttaaagccg taaaaggcag gttgtccccg ggaggttttg
    13621 tgcatcctga taccgatcgg ttgttgttgc tcaaaggaca gaaggccgct gataaacgag
    13681 attacctgtt tgtcgctatt gacgattttt atactctgcc actttgccgg acaaaaccgc
    13741 agacagtgct gccaagtttc tgaccgaaca tctggccgac ccctgcttgt acctgattga
    13801 gtacgtttac tctgacaatg ataggtaata taaagagccg tccaacatgc tttcggtgca
    13861 gcttgttatg ataacgggat tggttggagg cttgcccgat ttgcttgtcc gcagaccaac
    13921 ggtaaggcgg agcgggttat ccgtaccttg atggagatgt ggcatgagga acagtcgttt
    13981 gacagtccgg aacatcggca aaaggagttg tgctgcttta ttaatttagg ggctgcccta
    14041 gatcactagg gaaattcaaa ttaagttaga attatcccta tgagagaaag tcgtctaagc
    14101 cggtaataaa caaaataaac tcattttgtt atcgtggcta tgatgtattg gatgcgggcg
    14161 aatttagcca tttccgtatc aatcacagca cacattttgc cgaacgacaa aaccatatta
    14221 atggaactga gaacttttgg aatcaagcaa aatgtcattt acgcaagttt aacggcattc
    14281 ccaaagcgca ttttgagctg tatttaaagg agtgcgaatg gcgttttaac aacagtgaaa
    14341 taaaagttct tgttccattt taaaacaatt agtaaaatcg agtttatcct agttgtccag
    14401 ggcagcctca tctctttata gatagctgct taccaagtat ccttaattgc taattcaagc
    14461 caatcttcaa aactattaaa aaattcatgg ttttctatat tatctaaatt aatgacaata
    14521 accctagaat ttcctgttac atcattacca tcaaaataag cttcccattc tccattacta
    14581 gcgaatggga taagatttaa atcaggcagg atagaatttc taaatatata agctacttct
    14641 gccccttctg ttccataatt ctcaaactac cagtgaaaat tatgtggctg aatataaggt
    14701 attaaagatg tatcgttaga gatacgaaca tacttttcag gataacaaaa cctttcagga
    14761 agcatattac taggatataa attaaattca ttttgaataa tcatttcgtt accttaggat
    14821 cataaatact accattagga aactgggtag accaatttgg tggtgctagt tcattttcaa
    14881 tttgtttttt aattctaaat tgaatagctt ctccccatgt ttgcggcatc taatgctgct
    14941 tgctctgcat caatattact aaaaattgga ttagtctcaa aaatgaaaaa ttactgtttt
    15001 ttcatttttt atagcatatt caagtacatg aataatattt ttcactaagg gctctgtaga
    15061 aaaatctgaa ttttgtaatt tttcttttac agccaaaata ctttccttat ctattagcca
    15121 atccccctca cttggtccaa ataaccaatt atcggtaatt tcagaaacaa gtctccataa
    15181 tccacgattt tgatattcaa taatttgact ttccgtaatt tctacaactg tttcagattg
    15241 aatgttttta taagcccaat caatcatttc agtatattca ctaattgaaa cattgtcttt
    15301 taaacagaaa attaacaggt tgtgttcatt catttgtcta agttcttaat atgagtttgt
    15361 tgttgtaaat aatcttttgc ttgcttacct tgtaaattaa aggattaacc gcaccttgga
    15421 tgatgccgcg aatatcatcc atacggttag cccaccaatt tttattgggg tcgttttccc
    15481 atatcagttt tcctgcttca tcagcacaat tagggttggc attgatgccc acccagttcc
    15541 agcctgaaaa ggatttttaa tatacggcaa aaggtgggca acaagtgccc accttaccta
    15601 gctaacattt atcccaagta tcatatattt ttttaagttc tttatccaaa gaaagaactt
    15661 tattttttca acaccatgaa ttatctcaaa atctctacaa tccacgcata cgcctgaata
    15721 aatttctcct acaattgctt gaataatata caactcatct ttatcgagac taattttaac
    15781 tgaactctct tcaaaattaa ttaatttcat gaataattcc ttttatttta attcgtcatc
    15841 aaaataccgt ttgcctgttt ttggtctaaa tgctgtccac catctttagt cttggatatt
    15901 tagattataa tcgtcaaatt cccagtcaaa atattttgcc aaatttgata tagacttatc
    15961 ttcagacgtt gaattacata tttccaatat atcatttatc aattttaata tgtgttcaat
    16021 attaaaacgg tttgtaacaa ttgagttttt taatgacatc ggtctatgta tatttgaaag
    16081 taaccattcg gtgctatata tataaacata aaaataatca gcaccattac tttcatcaaa
    16141 acctattgat aatgtcaaaa gtatacaaaa attttctaac ttgtcttgtg ggaaatcttc
    16201 caaatcaaag tcagaacaat cataattttt taattttaat ctcataatat attgagatag
    16261 cttaggaatt ttattttctc agattgaggc aactcatcaa aaccaagcca acaataaaaa
    16321 ttgttatatg attttaaaaa atcaacaatt tttatttttc ctaaaaccaa atcttcgact
    16381 aactgggttg cataaataat atcatttatg attttaacag gtaaattatt tgataggtaa
    16441 ccatgggatt ccgtcatcat tgatttctct aggtgcttat ttatctgatt ggtaagtctt
    16501 ttgcgtaagc ttctttcgcc tccgcaagtg tcatttcatt agggccacca tctatattag
    16561 tttctcctgt attttgccac tgacaaacgt cacagatatc atagtccata acttcagttc
    16621 cgcaaacagg acaatgtagc catatttctc cgtcaatttc ccatgtcttt tttgaattct
    16681 ccatcataat acttctttcc taagtttggt ttaaacattg ttttgatttt gaatgttttt
    16741 ggattggcca aaacatagat attgttattg atatcatagc gcactcgtct atgttccgtc
    16801 tgataaccta atatcttgtc cgatgtctgt tttgataaca aatcagacgc cattttttgg
    16861 tagtcttcaa tggttatatc gccaaattct tttccatgag ctttgaaatg tccatttaaa
    16921 gatttctcag taggaaactt ggactttgtc catctgatgc ggtcttgaat tgttcctgtc
    16981 ggagaaatag atgtatcagg tttaacatat gaggcaggta agccgatatt gttatcagag
    17041 tgtgtagggt gttgctgcgt acttggtttt ggtggcttat tcccacctgc catctcccca
    17101 tctaaagtct gcatagggcg ggcagtaggt gttttatagc cggtattttt aacccaatcc
    17161 catttggtcg ggttaagttc tactttttta ccgccccaaa ccgtacctcc agtcaggtgt
    17221 agttggatgg ggattcattt ttaataatat cttcaagcaa gtgaaatgta agaaaagcgt
    17281 agatacttga aggccggcta tctgattggc aaaaagaaat ttcggagata aagaatttcc
    17341 gtttatattt ttcataaatt aaatcaacac cttgttttac ataaaaacta acttcctcag
    17401 gaaggatatt tataacattc tctcctttta cttttatagt taatggtttg atttttatgt
    17461 tttcatgaat atctgatagg attatttcta aaaaattatt tttatttcca gtcattctgg
    17521 ctattttatg tatatttcca ttgtgttcga ttatcatttt attttttcca aggatttcct
    17581 tctatattga tagtttatct aaagatttca tttaaaatat cctgactaat ttcttgttta
    17641 tataattcta caattttctg tttctgtgta tttgtataaa atttttcgtt ggtatctgct
    17701 tgatatttta attgtctcaa tatttccatg atttcatatt ctgatttttc atcatattct
    17761 tcaatcatgt aatcaacaaa ttgttttaga atatcaaagg cttcgtaact ttccaaaatt
    17821 ttaatatgtt tatcggcaaa attggacata taccattcgt cagtgattgg attagtattt
    17881 aaatcatcta aaaaaacatt tacatcatct aaaaacatga ctaatcctta aacttaaaat
    17941 ctgttttttc accggttctt gtgttttcaa taaacttaaa atctgttttt tcaccggttc
    18001 ttgtgttttc aatgtaatgt atttcaattt tgtttacgtt ttgaacacgc ttaacccaac
    18061 cgtcttttgc taaccaacca tttttagtat cggacatagg aggtattctc gcaggagttt
    18121 tgccctgtgg gtttgcctta acttcttcca ttgctaattg ttcctttaaa ttattaggta
    18181 ttgtacgacc ggttgatttt tcaacagatt gtgtccctat ttttaatgat ttgtttcccc
    18241 cagccatttc cccgtctata gtctgcatat ggcgaacagc aggtttttta tagccggtat
    18301 ttttaaccca atcccacttg gtcgggttaa ggtttacttt tttaccgcct caaaccgtag
    18361 ttgcggcctc tgctatggca agggcggttt gggcagtagc tgtaatgttc ggatgggcat
    18421 cagcccattg tttggcagag ttgataccgt cttttaccgc aaaagcactg tcttgtaatg
    18481 cggttgcggc cgcaagttgt gcttccggac ttaaatttcc taaattgtga ataccctgta
    18541 aagttttccg tgctgccgca taggttaccg ggtttaccgc actgtctgta attgccccaa
    18601 ctcccaatcc ctgaaaaccc gttagaaaag gattggctgc accttggatg atgccgcgaa
    18661 catcatccat acggttaccc caccaatttt gattggggtc gttttcccat atcagttttc
    18721 ctgcttcatc cgcacggctg agaaaaccgg aagcggcacc ggcattttct tttagccagc
    18781 ggtctgaaaa cggggcttgc ggaacagtgt ttatctttgt ttttgttgaa gttcctttga
    18841 tatggtaact gtatatatcc cttgctcctc cggggggcgg ataatcgctg ccttgcggtc
    18901 cgtcatatcc atcctccgga tgaatttccg accctgttcg atgaagttgg taaacagtaa
    18961 aaccgccgtc tacaccgccg ctgaaatcag aagtgctttt tgaatcatga tgatcgaacg
    19021 gactgtgtac ttcatgtccg tgccctgaaa aatgggtttc ataaccgata acaccttcaa
    19081 accctgtccg ttcgtgtgta ataggcagta caggactgac cgcagttgca tcaaatgtct
    19141 ggacggcgta aacccgcttt ttaacactgc cgcgagcatt accaaacaga tggtatttac
    19201 cacccggttc gtagtgtttt gcctgcatat catcgcgcaa acgggcatcc aaaccgttcg
    19261 catgactaat ggggatttgc agcaacgata ttgctgctgc aaacagcatc atgaattttt
    19321 gaataggcaa attcattttg ttttccttgg tagcaagcgg ttatggtagt gtgaatcaag
    19381 gttgtccttg tctatgttgt cgcactactt catcgctgta tccatacccc tcatgactgt
    19441 tatcagcctc tacggatggg gcagagttac ccgtatgatt gccgtatggt cggatacttg
    19501 tatccgacaa aaatatttga cgcgtctatc gtttccgaaa tgccgctgtt ggaaaatgtc
    19561 ggattcgaga atccgaccta cggctgctac caaccatcat tttcagcttc ttttagccaa
    19621 gtatcaaaat tttcaaaagt ctcgtaatat tttgtattat ctaaattaac tactataatt
    19681 ttaggattac ctgatttatc tgaaatatca aaacaggcag cccactcttg gtttctagca
    19741 aaaggtaata ggtttggaat gccagttatt tcaaaataaa tgtcaattac ttctgatata
    19801 ttgctttctg cattttcaaa ccaccaagga aaagaatatt gttcatcgta gttaatggca
    19861 tgagtggatt gagctaattt taaataactt tgcggatatt taaatccttt aggaagcatt
    19921 gcttcaggat acggaacaaa actattttgt tttatcattt cctaagtacc tttacatcat
    19981 aaatacttcc gttaggaaat cttttgctcc acccttctgg aactcctttt tgtgtttctt
    20041 gctttctaat tcgtagctgg atagcttctc cccaagtttg aggctcaata ccatcatgct
    20101 taaacctgat tctctctttt gatgataatc ggtcattaaa attatgaaca ttcgcttcca
    20161 cttttacccc tgcttcacgt gctgccataa tacgcgtgtt atccatacta gtaggtgctc
    20221 catcaggcat attaacaaca tcaaccctgt cgccaaccca tgattttttt ctttcatttc
    20281 ggctacaata gaatcatagt tgtaatttgc actaggctct gacaaaaatt tgggttgtta
    20341 agttgtcatt tttttataaa ttccagcaaa ctgttgttgt aaattatcta cttcatgttt
    20401 tctaacacca gatacatttt caaattctct tgaatctaca catacgcccg catatatctc
    20461 attcaagata gctcgtaaaa caaaaacttc atcttgagtt agtgaaatag ttgagttttg
    20521 attatttatg gatagtatat tcatggcttt ttcctataaa tcatcataat attttttacc
    20581 tgatgttggt ctaaatgctg tacctccatc gtcagaattt ttatctcgga taactattgt
    20641 ccctgtttta tcatcccaat acgcagttct accgcgaggt aactctttca tatttgttgg
    20701 atggctaaca atattttcaa tatgccgagc aaaatctgct ggtgaattga tatttaaatc
    20761 cgtaaattct tgttgtctta tgacatgctt gttataagca tgcccactac taatttgttc
    20821 tccaattagt tgcgcttgta aagacggttg tgtggaagca tctgctttgc tgttggacgt
    20881 tatagattta ggcggtctat tcccccctgc catctcccca tctacagtct gcatatggcg
    20941 ggcagcaggt tttttatagc cggtattttt aacccaatcc catttggtcg ggttaagttc
    21001 tactttttta ccgccccaaa ccgtagttgc ggcctctgct acggcaaggg cagtttgggc
    21061 tgttgcagtt atattcggat gggcatcagc ccattgtctg gcggaattaa tgccgtcttt
    21121 taccgcaaaa gcactatcct gtaataggct cgcggcagca agttgtgttt ctggacttaa
    21181 atttcctaaa ttattaatac cttgtagagt ctgctgcgca gagatgtagc aactgtattt
    21241 ttaccccgtc gggtaaaaat acagttgcta cggctgctga gaaagcagtt actattttcc
    21301 cagtggctca aaattaattg tttcagcaga tatttttata ataatcccta catcaataat
    21361 aatttcacta taaaattggt atttatatag ctctatttcg ggtataaaca agggaaacaa
    21421 gctatcttcc ttatcctcat aatctacgaa attatttgta tctaatttaa aattcaaaat
    21481 attactgcat ttgatatatc cgaaataatt accttcaatt gtatctgtaa aagataatgt
    21541 aagggtattt ccattatcgg aaaagattat ttcataaagg cttacattgt gaaaaatatt
    21601 ttttttcatt tgacaaaata tccagaatga tgatgattat ttggcaatgg attgccttca
    21661 taatcttgtc gttgcggata ttttccctgt acaccatcgg ttcgcggcac tctttgggtt
    21721 cttataacct ctccatttgg acgaatatct attttcacgc catcaggacc tttccatgtt
    21781 tcatattgag ccgcatctga accagtacgt ctaaaacctt gttcttgcaa tgcttgacgt
    21841 aattcatctc gcgtagttgg tctgatagat ttaacaactg gcttgtttcc accagccatt
    21901 tccccatcta aagtctgcat aggtcgggca gcaggtgttt tataatcggt atttttaacc
    21961 caatcccatt tagtcgggtt aagttctact tttttaccgc cccaaaccgt acctgcggcc
    22021 tctgctacgg aaagggcagt ttgggctgtt gctgttatat tcggatgggc atcagcccat
    22081 tgtttggcag agttgatacc gtcttttacc gcaaaagcac tgtcttgtaa tgcggttgca
    22141 gccgcaagtt gtgcttccgg acttaaattt cctaaattgt gaataccctg taaagttttc
    22201 cgtgctgccg cataggttac cgggtttacc gcactgtctg taattgcccc aactcccaat
    22261 ccctgaaaac ccattaaaaa aggattaacc gcaccttgga cgatgccgcg aacatcatcc
    22321 atacggttag cccaccaatt tttattgggg tcgctttccc atatcagttt tcctgcttca
    22381 tccgcacggc tgaaaaaacc agaggcggca ccggcatttt cttttagcca acggtctgaa
    22441 aatggggctt gagggacaat attagtcttt gtttttgttg aagttccttt gacataatag
    22501 ctgtatatat cccttgctcc tccggggggc ggataatcgc tgccttgcgg tccgtcatat
    22561 ccatcctccg gatggatttc cgaccctgtc cgatgaagtt ggtaaacagt aaaaccaccg
    22621 tctacaccgc cgctgaaatc agaagtgctt cttgaatcat gattatcgaa cggactgtgc
    22681 acttcatgtc catgtcctga aaaatgggtt tcatagccga taacaccttc aaatcctgtc
    22741 cgttcgtgtg taataggcag tatggggccg accgcagttg catcaaatgt ttggacggcg
    22801 taaacccgat ttttaacact gccgcgacca tcgccgaaca gatggtattt gccacccggt
    22861 tcgtagtgtt ttgcctgcat atcatcgcgc aaacgggcat ccaaaccgtt cgcatgacta
    22921 atggggattt gcagcaacga tattgctgct gcaaacagca tcatgaattt ttgaataggc
    22981 aaattcattt tgttttcctt ggtagcaagc ggttatggta gtgtgaatca aggttgccct
    23041 tgtctatgtt gtcgcactac ttcatcgctg tatccatacc cctcatgact gttatcagtc
    23101 tctacggatg gggcggagtt acccgtatga ttgccgtatg gtcggatatc ggagaaatcg
    23161 accattaatc cttccgtcgg tttaattcct ttgcttactt tatacggccc catccacaat
    23221 gcgtaatttt ctttataggc agcttcaaac gcattggttt tgggtttgat gagcaatttt
    23281 ttattggttc tgtctactgc gaaatattcc agttttgttt gggctttcag tgtttcggca
    23341 ttgtataggt gcatttcggt tctgttgcgt atcgttccga atacgtcgat gttaataaac
    23401 acatctgtat cggcattggc aggagaaaca acgtctatgc cgcgcaggaa aaatacggtc
    23461 tgtaccaagt gggaaagaaa ggcagtgtcg cgcgggttag tcgtcaaggt ttcatttcga
    23521 taatccccca tcccgccaat atttaagccc agactgcttt tacttccgct accgtctgat
    23581 tgggtgcgag agagtgcagg ggcattaagt gtagataaag aagtggttaa acctgtcaaa
    23641 ccgcctgatg ttgtttcagc ggtggtttcg taacgtggat aggtgtaatc ggtacggacg
    23701 gcagggctgt ttatgtattc gccacgaatc agtgcatcaa tggagtagcg accccctgtc
    23761 aaactgcctg aaccttggtc gcccatagtt gcaatgtaca atgcaacttt tcgtccgtgt
    23821 aatgcctgta aatccatgtc tttaacggca gctctggcag aagcggccac aagttcttgt
    23881 tcgaccgcaa agcgtttacc tccgccatgc gatggaatac ctgtcagtgt cccgcaggcg
    23941 gataaaataa aaactgaaaa aagaataggt atcagcagcc gtgcttgcat agattttctc
    24001 ctttgatgaa aaacaaattg tatcaaaatt gtaaatatag tggattaaat ttaaaccagt
    24061 acggcaaggc gaggcaacgc cgtactggtt taaatttaat ccactataat ctccatataa
    24121 taaatggctg tctgaagccc atatgcgact atatctgccg acactatggt caatcagcag
    24181 tgaaccagcg taccttcgtt ttcaccggtt actacacgtt tgagcgagcc ttctttagca
    24241 atgccgaata cgacaatatt gagcttgcgt tcgcggcaga gggcgaaagc ggtggcatcc
    24301 atcactttga ggtttttcaa caaggcttcg tcaaaagtaa tggtttcgta gcgcgtggcg
    24361 gatgagtctt ttttggggtc tgcggtgtac acgccgtcaa cgttggtggc tttgagcatc
    24421 acgtcgcagt tcatttccgc accgcgcaat gcggcggcag tgtcggtcgt gaagaacggg
    24481 ttaccggtac cggcggcaaa aatcacgact ttgccttctt ccaaatattg gatggctttg
    24541 gggcgggcgt aggtttcagc gatttgctgc atagacagtg cggattgtac gcgcgctttg
    24601 atgcctaaag tttcaaatgc gtctttgagt gccaacgcgt tcatcacggt cgccatcatg
    24661 cccatgtagt cggcggtggc gcgatccatg ctgcctgctt gggcggatac gccccggaaa
    24721 atattgccgc cgccgacaac aataccgact tgcacgccca ttttaacgac ttcggcaatt
    24781 tcgccgacag tttgaacgat ggtatcgtga ttgatgccga acggatcgga acccatcagg
    24841 gattcgccgg agagtttcag taatacgcgt ttgtatttga tttgctgtgt catggatacc
    24901 ttgctttctt gaatgtcgtc ggtcggagtt gggtcgtctg aatttcaaac ggtcggaata
    24961 ttaatgatag ggtgtgtcgg tttattattg ttttgtttta agacggtctg aatattcggt
    25021 cgtcttaaaa taggtaccgc aaacggtttt ctcaaaaaaa agcaccctga ttcgtttgga
    25081 atccaggtgc tttctttttc ataagtgcct tacactttgg cggctgcggc tacttcggct
    25141 gcgtagtcga caacggcttt ctcgataccg tcgcctactt tgtagcggac gaagctgacc
    25201 acttcagtgc cgttttcttt ggcgaattgg gcaacagttt gatcggggtt catcacgaat
    25261 gcttggccgt tcagagtgat ttcagccaag aatttgcgaa tacggccttc aaccatttta
    25321 gcggcgatgt cggcaggttt gccggaagcg atggcttgtt cggtgtagat gtggcgttct
    25381 ttttcaacgg tttcggcatc tacttcggct tcgcttacgc attgtggttt agcggcaaca
    25441 atgtgcatac cgattttgcg tgctacgtct tcagagcctt tgtactcaac caatacgcct
    25501 tcggtcgcca atgcgccgtg gatgtaggca accagttggt tggcagtgtc gatcacttgg
    25561 aagcgacgga cagacatgtt ctcgcccaat ttggcgatga tggctttgcg ttctgcttca
    25621 accagttcgc tcagttcttc aacagaagcc ggttttttct cggcggcagt tttcgcaacg
    25681 aagttggcaa attctacgaa gcccacgtct ttagcaacga agtcggtttc gcagtttact
    25741 tcgaccaatg cgccgacatt gccgttgatc gcgtaagcca atacgccttc ggcagcagta
    25801 cggccagcca gtttaccggc tttcgcacca gatttaatac gcaggatttc ttcggctttg
    25861 tcgaagttgc cttcggcttc aaccaaggct tttttgcatt ccatcatgcc caggccggta
    25921 gcagcgcgca ggtcggcaac catttttgca gtaatttctg ccattttgaa tctcctagat
    25981 ttttgggaaa tacggaaagg attttccgca ttcgggaatc gtgtgccgcg aacggacgtt
    26041 cagacggcat atttgagaaa aggggcataa cgcccctctt cggtttgccg gattactcgg
    26101 cagcggcttc ttgggcagcg gctacggttt cttgcagcgc ttggtttttg ccttccaaaa
    26161 ctgcgtcagc gatgccgcgg cagtacaggc ggatggcttt ggcggagtcg tcgttgccgg
    26221 ggatgacgta tttcacgccg tcagggctgt tgttggtatc gactacggcg ataacgggga
    26281 tgcccagttt ttcagcctcg accagagtac ctttttggta gccggtatcg ataacgaaaa
    26341 tcgcgtcggg caggcctttc atgtttttga taccgcccaa agaacgttcc agtttttcaa
    26401 catcgcgttg catttccaga atttcttttt tgctgaaacc gctttcggca gcattttcca
    26461 aggctgcggt tttttcttcc aggcgtttga tggattgctt aacggttttg tagttggtca
    26521 gcataccgcc caaccaacgg tgatcgacga aaggcatgcc ggcgcgggtc gcttcttcgc
    26581 ggatgatgtc gcgggcttgg cgtttggtac ctacgaacaa tactgtacct ttgttggcaa
    26641 ccagacgacg tacggcttct tgcgcgtctt ggaacatcgg cagggttttt tccaggttga
    26701 cgatatggat tttgttgcgc gcaccgaaaa tgtattgagc cattttcggg ttccagaaac
    26761 gggtttggtg gccgaagtgg acaccggctt caatcatctg acgcatagta atttgagaca
    26821 ttttgatttc cttgaaaggg ttaagggcac acattcaatc gcatagaact tgatagaccg
    26881 cgcctattgt caagcaccct tcggcgaaag tgggcataag ttttaaaaaa taaaagagtg
    26941 tttccgaaat ggaaaactga cggattatag cggataagag gtcgtctgaa aagggattca
    27001 gggttttgtg ccgctgtctt tttaccgttg tgcacgctgc tgttcgaatt ctgcctgttc
    27061 ctgttgcttc atcaggcggt tgcggcggcg gatggtcacg acaaagaccg tgaacacggt
    27121 gggcagcacc gcccaaaaaa ccaaataaat caaggtgcgc gcaatactcg gctgcgcggc
    27181 ggaaaacatc acggcaacaa aaagatagcc gatggcgaca atgtgccaca ttgtgtgcgt
    27241 cctgtttgaa tgttaacaaa atcgttataa tcgcaacatt ctaccgcaat cctgaaaggc
    27301 cgtctgaaat gaggaacgag gaaaaacacg ccctgcgccg cgaattacgc gggcggcgtt
    27361 cgcaaatggg gtgggacgtg cgggcggcgg caacggtaaa aattaaccgc ctgctcaaac
    27421 gttatatcaa gcacggtcgg aaaatcggcg tgtattggcc gatgggcaag gaattgcgtt
    27481 tggacggctt tgtccgcgcg gcgcaaaaac gcggcgcaaa actttatctg ccttatatcg
    27541 aaccgcgttc gcggcggatg tggtttacgc cgtatcctga aagcggaatg gaacgggagc
    27601 gcatacgggg cagggcgagg ttgaacgtgc cgcagtttgc agggcgcaaa atccgcgtgc
    27661 acggtttgtc ggtattgctc gttccgcttg tcggcataga ccgcgaaggc taccgtttgg
    27721 ggcaggcagg cggctattac gatgcgacgc ttgcagcgat gaaataccgt ttgcaggcaa
    27781 aaaccgtggg cgtgggcttt gcctgccagt tggtggacag actgtcggtc gaggcgcacg
    27841 acctgccgct ggacggtttt gtgtcggagg cggggatatt gtgtttttag ccgttcagac
    27901 ggcattttga acggaaatgc cgtctgaagg cggaatattg ggagaaaagg atgtcggaaa
    27961 ggataatact gccggtcttg tcgttgggcg gcgttcgtct cgaaccgttg gatgtgcatc
    28021 atgaaatggg tttgcgcgag gcggtttgcg atggggaagt ttggaagctg ggagtaacgt
    28081 ccgcgcccca tccggatagg gtggcagact atatcgggac agcattggca acgcgtttgg
    28141 cgtttgcggt tgtcgatgaa gaggcgggca gggtggtcgg gacaacggcg tattatcatt
    28201 ttgaaccgca gattccgcgt ttggatatcg gattcacatg gtatgcggca tcggcacggc
    28261 ggacacggat aaacacctgt tgcaaaatca tgctgttgga ttacgcattt gatgttttgg
    28321 cttgccgttg cgtgggatgg cgcacggata ttctgaacct tgcctcgcaa cgtgccatcg
    28381 aacggctggg tgcggaaaaa gacggcgtgc tgcgtatgca tatgctccgt aaagacggca
    28441 gtgtgcgcga tacggtcgtg tacagtatgc tgagggaaga ttggtgcaaa aacagggaaa
    28501 tcctgaccgg caggttggca gggtatggcg tgcaggtgtg aggcagtatt ttca
//
LOCUS       DAWEWP010000006        28454 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWEWP010000006 DAWEWP010000000
VERSION     DAWEWP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.006"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            24..1106
                     /locus_tag="WGN53_000175"
     CDS             24..1106
                     /locus_tag="WGN53_000175"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ0139215.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            1221..1493
                     /locus_tag="WGN53_000176"
     CDS             1221..1493
                     /locus_tag="WGN53_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ0139216.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWFGKACQNGDQDGCDIYQRLKAGY"
     gene            1583..2920
                     /locus_tag="WGN53_000177"
     CDS             1583..2920
                     /locus_tag="WGN53_000177"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ0139217.1"
                     /translation="MTLENKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATKDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGKKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            3046..4371
                     /locus_tag="WGN53_000178"
     CDS             3046..4371
                     /locus_tag="WGN53_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ0139218.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            4375..5442
                     /gene="murG"
                     /locus_tag="WGN53_000179"
     CDS             4375..5442
                     /gene="murG"
                     /locus_tag="WGN53_000179"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ0139219.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDDARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            5596..7005
                     /locus_tag="WGN53_000180"
     CDS             5596..7005
                     /locus_tag="WGN53_000180"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ0139220.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            7118..8032
                     /locus_tag="WGN53_000181"
     CDS             7118..8032
                     /locus_tag="WGN53_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ0139221.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAESCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            8022..8750
                     /locus_tag="WGN53_000182"
     CDS             8022..8750
                     /locus_tag="WGN53_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ0139222.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            8836..10080
                     /gene="ftsA"
                     /locus_tag="WGN53_000183"
     CDS             8836..10080
                     /gene="ftsA"
                     /locus_tag="WGN53_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ0139223.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            10199..11377
                     /gene="ftsZ"
                     /locus_tag="WGN53_000184"
     CDS             10199..11377
                     /gene="ftsZ"
                     /locus_tag="WGN53_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ0139224.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            11516..12826
                     /locus_tag="WGN53_000185"
     CDS             11516..12826
                     /locus_tag="WGN53_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ0139225.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            13452..14327
                     /gene="prpB"
                     /locus_tag="WGN53_000186"
     CDS             13452..14327
                     /gene="prpB"
                     /locus_tag="WGN53_000186"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ0139226.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            14413..15567
                     /gene="prpC"
                     /locus_tag="WGN53_000187"
     CDS             14413..15567
                     /gene="prpC"
                     /locus_tag="WGN53_000187"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ0139227.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            15660..16382
                     /locus_tag="WGN53_000188"
     CDS             15660..16382
                     /locus_tag="WGN53_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEZ0139228.1"
                     /translation="MDKDKGSYAGIPVQNTKELVWQKIQDIPISECGEPLVSVGNTGR
                     IRVNPVYFSQGIEGAINSVLLRRSVADRLTQVVSLLPEVYGLEIFDGWRPVIVQRALR
                     ENMKQEIEKRHPEYDEVQVREALDCFAADPDRAGMPLPHLTGGSVDVGLFDIKSGKSL
                     DMGSEFDATGHLSYSDALEKEGEYFSSARNLRRILIHAMKEAGFSNLPTEWWHFDYGN
                     QNWAYFTQQSNAVFGAVEEQDT"
     gene            16408..17613
                     /locus_tag="WGN53_000189"
     CDS             16408..17613
                     /locus_tag="WGN53_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HEZ0139229.1"
                     /translation="MQTLISADNHLLLWAFVMLAAAAAIFIEQNSRLAGKVPGAVLAL
                     LIALIASNLGVIPTDAPVFDAVWSYIVPLAIPLLLFQLDLHALFKESGRMLFIFLISS
                     LGTVIGTLVSFALLKNYIPELDKVAGMISASYTGGGVNFAAMSAKLNPAKDVIAATIV
                     ADNLMMACYFFILIGISSSYWVRKVWGSPYQDKLEQEYHDPSVNKAAEYWKPKSISLQ
                     SIALSLAASILLVAVSFSLSSFLQNHFQSVDGVALKMLTGLISDKYLLLTTFTLAVVL
                     FFRKGIQKLDGSQELGTYCIYLFFVVIGVPASIPLIIKTAPLLFVFTLIIAVLNLLLT
                     LVLGKLFKFSIEEIVLACNANIGGPTTAAALAISKGWRDLVGAILLIGTVGYIIGNYI
                     GSFVYALLS"
     gene            17716..20322
                     /gene="acnD"
                     /locus_tag="WGN53_000190"
     CDS             17716..20322
                     /gene="acnD"
                     /locus_tag="WGN53_000190"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ0139230.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRKAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATVDLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLSGMRPLAILPDNITTDHLSPSNAILASSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVRNEDGSVRQGSLARVEPEGQTMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVVGERKPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            20675..21844
                     /gene="prpF"
                     /locus_tag="WGN53_000191"
     CDS             20675..21844
                     /gene="prpF"
                     /locus_tag="WGN53_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ0139231.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            22259..23458
                     /locus_tag="WGN53_000192"
     CDS             22259..23458
                     /locus_tag="WGN53_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0139232.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSIVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            23565..24185
                     /locus_tag="WGN53_000193"
     CDS             23565..24185
                     /locus_tag="WGN53_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ0139233.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(24312..24755)
                     /locus_tag="WGN53_000194"
     CDS             complement(24312..24755)
                     /locus_tag="WGN53_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ0139234.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            24929..25297
                     /locus_tag="WGN53_000195"
     CDS             24929..25297
                     /locus_tag="WGN53_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0139235.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            25342..26496
                     /locus_tag="WGN53_000196"
     CDS             25342..26496
                     /locus_tag="WGN53_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0139236.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(26553..27425)
                     /locus_tag="WGN53_000197"
     CDS             complement(26553..27425)
                     /locus_tag="WGN53_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ0139237.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            27510..28322
                     /locus_tag="WGN53_000198"
     CDS             27510..28322
                     /locus_tag="WGN53_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ0139238.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTISAGRAVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRTRAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         6560 a         7232 c         7904 g         6758 t
ORIGIN      
        1 acaaaacaaa aaaaggaagc cccatgtttt tatggctcgc acatttcagc aactggttaa
       61 ccggtctgaa tatttttcaa tacaccacat tccgcgccgt catggcggcg ttgaccgcct
      121 tagcgttttc cctgatgttc ggcccgtgga cgatacgcag gctgaccgcg ctcaaatgcg
      181 ggcaggcagt gcgtaccgac ggtccgcaaa cccacctcgt caaaaacggc acgccgacga
      241 tgggcggttc gctgattctg accgccatta ccgtgtccac cctgttgtgg ggcaactggg
      301 caaacccgta tatctggatt ctcttgggcg tattgctcgc cacgggcgca ctcggttttt
      361 acgacgactg gcgcaaagtc gtctataaag accccaacgg cgtgtccgcc aaattcaaaa
      421 tggtgtggca gtcaagcgtt gccattatcg ccagtttggc attgttttac cttgccgcca
      481 attccgccaa caatattttg attgtcccgt tcttcaaaca aatcgccctg ccgctgggcg
      541 tggtcggctt tttggtgttg tcttacctga ccatcgtcgg cacatccaat gccgtcaacc
      601 tcaccgacgg cttggacggc cttgcgacct tccccgtcgt cctcgttgcc gccggcctcg
      661 ccatcttcgc ctatgccagc ggccactcac aatttgccca atacctgcaa ttaccttacg
      721 ttgccggcgc aaacgaagtg gtgattttct gtaccgccat gtgcggcgcg tgcctcggtt
      781 tcttgtggtt taacgcctat cctgcgcaag tctttatggg cgatgtcggt gcattggcat
      841 tgggtgccgc gctcggtacc gtcgccgtca tcgtccgcca agagtttgtc ctcgtcatta
      901 tgggcggatt atttgtcgta gaagccgtat ccgttatgct tcaggttggc tggtataaga
      961 aaaccaaaaa acgcatcttc ctgatggcgc ccatccatca ccactacgaa caaaaaggct
     1021 ggaaagaaac ccaagtcgtc gtccgctttt ggattattac catcgtcttg gtgttgatcg
     1081 gtttgagtac cctcaaaatc cgctgaacct atgccgtctg aacacctttc agacggcatt
     1141 tgaacgcgca ataaacctgc ggcgacaatc cgcccagccc tatcgttaac ggcggcttat
     1201 actaaaacag aagtaaaacc atgaaacaga cagtcaaatg gcttgccgcc gccctgattg
     1261 ccttgggctt gaaccaagcg gtgtgggcgg atgacgtatc ggattttcgg gaaaacttgc
     1321 aggcggcagc acagggaaat gcagcagccc aatacaattt gggcgcaatg tattacaaag
     1381 gacgcggcgt gcgccaagac cttgtgctcg ctcaagaatg gtttggtaag gcttgtcaaa
     1441 acggagacca agacggctgc gacatttacc aacgcctgaa ggcaggttat tgaacagccc
     1501 gcgatgccgt ctgaaagcgg cttgggcagg ggcggacatc cccccacctg cccaatatga
     1561 tttgttttag gacaaaacca aaatgacgct tgaaaacaaa aaaatcctcg tcgccggact
     1621 cggcggtacg ggtatttcca tgattgccta tctgcgcaaa aacggcgcgg aggtggccgc
     1681 ttatgatgcg gagttgaagc ccgagcgcgt gtcgcaaatc ggtaagatgt ttgacgggct
     1741 ggtgttttac acaggccgtc tgaaagatgc gctggacaac ggtttcgata ttttggcact
     1801 cagccccggc atcagcgagc gtcagccgga tattgaggcg ttcaagcaaa acggcggacg
     1861 cgtgttgggc gacatcgaat tgctggcgga cattgtgaac cgccggggcg acaaggtaat
     1921 tgcgattacc ggcagcaacg gcaaaaccac ggtaacgagc ctggtcggct atctctgtat
     1981 caagtgcggg ctggataccg ttatcgcggg caatatcggc acgccggttt tggaggcgga
     2041 atggcagcgc gaaggcaaaa aggcggacgt gtgggtgttg gagctttcca gcttccaact
     2101 ggaaaacacc gaaagcctgc gcccgacggc ggcgacggta ctgaatattt ccgaagacca
     2161 tctcgaccgc tacgacgact tgctcgacta cgcgcatacc aaagccaaga ttttccgtgg
     2221 cgacggtgtg caggttttga atgcggacga tgcgttctgc cgtgccatga aacgtgccgg
     2281 gcgcgaggta aaatggtttt cgttggaaca cgaagccgat ttttggttgg aacgtgagac
     2341 aggccgcctg aaacaaggca atgaagattt gattgccacg aaagacattc cgttgcaagg
     2401 cttgcacaac gccgccaacg tcatggctgc cgtggctttg tgcgaagccg tcggtttgcc
     2461 gcgcgaagca ttgcttgaac acgtcaaaac cttccaaggc ctgccgcacc gcgtggaaaa
     2521 aatcggcaag aaaaacggcg tggtattcat tgatgacagc aaaggcacga atgtcggcgc
     2581 gaccgccgcc gcgattgccg gtttgcaaaa tccgctcttc gtgattttag gcggcatggg
     2641 caaagggcag gacttcacgc ctctgcgcga cgcgcttgcc ggcaaggcaa aaggcgtgtt
     2701 cctgatcggc gttgatgcgc cgcaaatccg ccgcgatttg gacggctgcg gtctgaatat
     2761 gaccgactgc gccactttgg aagaagccgt tcagacggca tatgcccaag ccgaagcgtg
     2821 cgatatcgtg ctgctcagcc cggcttgtgc gagttttgat atgtttaaag gctacgcgca
     2881 ccgttcggaa gtgtttatcg aagcgtttaa ggctttgtga tgccgtctga aatgcaaacg
     2941 ccgtcattgt tgggcggcaa gtaaagattt agaataccga tttgggatgt atcgtatgtt
     3001 cggacggcat tgtctgccgt ctgaaatttt tgccctttgc ggcaggtgca aacagactgg
     3061 caggtggttt ttttgaagat ttcggaagta ttggtaaaag tgggcgacgg tgtccacact
     3121 ctgctgctcg acaggccgat tgtgcgcgac ggcaggaaat tcgacgcgcc gcttttgtgg
     3181 atggtggtgc tgatgacggc gttcagcctg ctgatgattt attcggcttc tgtgtatttg
     3241 gcatcaaaag aaggcggcga tcagtttttc tatttgacca gacaggcggg gttcgtcgtt
     3301 gccggcttga tagcgagcgg tttgttatgg tttctttgca ggatgaggac atggcggcgg
     3361 cttgtgccgt ggatttttgc cctatccggc ctgttgctgg tagtcgtatt gattgccggg
     3421 cgcgaaatca atggcgcgac ccgttggata cctttgggtc cgttgaattt ccagccgacc
     3481 gagctgttca agctggcggt catcctttat ttggcaagcc tgttcacgcg ccgtgaagaa
     3541 gtgttgcgca gcatggaaag tttgggttgg cagtcgattt ggcgggggac ggccaatctg
     3601 atcatgtccg ccaccaatcc gcaggcacgt cgtgaaacat tagaaatgta cggccgtttc
     3661 cgggcgatca tcctgccgat tatgctggtg gcgttcggtt tggtgctgat aatggtacag
     3721 ccggatttcg gttcgtttgt cgtcattacc gtcattgccg ttggaatgct gtttttggca
     3781 ggattgccgt ggaaatattt tttcgtcctg gtaggcagcg tcttgggcgg gatggtgctg
     3841 atgattaccg ccgctcccta ccgtgtgcag cgggtagtgg catttttgga cccgtggaaa
     3901 gacccgcagg gtgccggcta ccagcttacc cactctctga tggcaatcgg gcgcggagag
     3961 tggttcggta tgggtttggg tgcgagtttg agcaaacgcg gctttctgcc ggaagcgcat
     4021 accgatttta tttttgccat catcgccgaa gaattcggtt tcttcggtat gtgcgtgctg
     4081 atattctgtt acggctggct ggtggtgcgg gcgttttcca tcggcaagca gtcgcgcgat
     4141 ttgggtttga ctttcaacgc ctatatcgct tcgggtatcg gcatttggat cggtatccaa
     4201 agtttcttca atatcggtgt gaacatcggt gctttgccga ccaaaggtct gacgctgccg
     4261 ttgatgtcct atggcggttc gtcagtcttt ttcatgctga tcagcatgat gctgctgttg
     4321 cgtatagatt atgaaaaccg ccggaaaatg cgcggttatc gggtggagta aatcatgggc
     4381 ggtaaaacct ttatgctgat ggcgggcgga acgggcggac atattttccc cgcgctggcg
     4441 gtggcggatt cattgcgcgc gcgcggccat catgtgattt ggctgggcag caaggattcg
     4501 atggaagagc gtatcgtgcc gcaatacggc atacgcttgg aaacgctggc gattaaaggc
     4561 gtgcgcggca acggcatcaa acgcaaactg atgctgccgg ttactttgta tcaaaccgtc
     4621 cgcgaagcgc agcggattat ccgcaaacac cgtgtcgagt gcgtcatcgg cttcggcggc
     4681 ttcgttacct ttcccggcgg tctggcagcg aaactcttgg gcgtgccgat tgtgattcac
     4741 gagcaaaacg ccgtggcagg cttgtccaac cgccacctgt cgcgctgggc gaagcgggtg
     4801 ttgtacgctt ttccgaaagc gttcagccac gaaggcggct tggtcggcaa ccccgtccgc
     4861 gccgatatta gcaacctgcc cgtgcctgcc gaacgcttcc aagggcgtga aggccgtctg
     4921 aaaattttgg tggtcggcgg cagtttgggc gcggacgttt tgaacaaaac cgtaccgcag
     4981 gcattggctt tgctgcccga cgatgcgcgt ccgcagatgt accaccaatc gggacggggc
     5041 aagctgggca gcttgcaggc ggattacgac gcgctgggcg tgaaagccga atgcgtggaa
     5101 tttattaccg acatggtgtc cgcctaccgc gatgccgatt tggtgatttg ccgtgccggc
     5161 gcgctgacga ttgccgagtt gacggcggcg ggattgggtg cgttgctcgt tccgtacccc
     5221 cacgcggttg acgatcacca aaccgccaac gcgcgtttta tggtgcaggc agaagcagga
     5281 ctgctgttgc cgcaaaccca gttgacggcg gaaaaactcg ccgaaatcct cggcagcctg
     5341 aaccgcgaaa aatgtctcaa atgggcagaa aacgcccgta ctttggcatt gccgcacagc
     5401 gcggacgacg ttgccgaagc cgcgattgcg tgtgcggcgt aaactgccga accatgccgt
     5461 ctgaaaagcc gttcagacgg catggatgtt ttttatttca atccgctata tatttgtcag
     5521 aaaactatgg cgcgcaaacg gtcagccctt taaaataacg cctttacgca tcgaaatcca
     5581 ccggaacgca acattatgat gaaaaatcga gtgaccaaca tccattttgt cggtatcggc
     5641 ggcgtcggca tgagcggtat cgccgaagtc ttgcacaatt tgggttttaa agtttccggt
     5701 tcggatcagg cgcgaaatgc cgctaccgag catttgggca gcctgggcat tcaagtttat
     5761 cccggccata ccgcagaaca cgttaacggt gcggatgtcg tcgttacctc taccgccgtc
     5821 aaaaaagaaa atcccgaagt tgtcgctgcg ttggagcagc aaattcccgt tattccgcgc
     5881 gccctgatgt tggcggagtt gatgcgcttc cgtgacggca tcgccattgc cggcacgcac
     5941 ggcaaaacca cgaccaccag cctgaccgcc tccatcctcg gcgcggcagg acttgacccg
     6001 actttcgtta tcggcggcaa actcaacgcc gcaggcacca acgcccgctt gggcaaaggc
     6061 gaatacatcg ttgccgaagc cgacgagtcg gatgcatcct ttctgcacct gacaccgatt
     6121 atgtccgtcg ttaccaatat cgacgaagac catatggata cctacgggca cagcgtcgaa
     6181 aaactgcatc aggcgtttat cgatttcatc caccgtatgc ccttctacgg caaagccttt
     6241 ttgtgtattg acagcgaaca cgtccgcgcg attttgccca aagtgagcaa accttatgct
     6301 acttacggtt tggacgatac cgccgacatc tacgccaccg acatcgaaaa cgtcggcgcg
     6361 caaatgaaat tcaccgtcca tgttcaaatg aaaggacatg agcaggggtc gtttgaagtc
     6421 gtgctgaata tgcccggcag acacaacgtg ctgaacgcat tggcagccat cggcgtggcg
     6481 ctggaagtcg gcgcatcggt tgaagcgatc caaaaaggct tgctcggctt tgaaggtgtc
     6541 ggccgccgct tccaaaaata cggcgacatc aagttgccaa acggcgggac cgcgctcttg
     6601 gtggacgact acggacacca ccccgtcgaa atggcggcga cccttgccgc cgcacgcggc
     6661 gcgtatccgg aaaaacgttt ggtactcgcc ttccagccgc accgctatac ccgcacgcgc
     6721 gatttgtttg aagactttac caaagtcctc aataccgttg acgcgctggt gctgaccgaa
     6781 gtttatgccg ccggtgaaga gccgattgcc gccgccgatt cccgcgctct tgcccgcgcc
     6841 atccgcgtgt tgggcaaact cgagccgatt tactgcgaaa acgttgccga tctgcccgaa
     6901 atgctgttga acgttttgca ggacggcgac atcgtgttga atatgggtgc gggaagcatc
     6961 aaccgcgtcc ccgccgcgct gctggaattg tcgaaacaga tttgaggcac acccgcctga
     7021 cagacggaac atcatataaa ggtcgtctga aaccgcaaat caggtttcag acgacctctg
     7081 gcaacaagca taaagcaatc aggaaagaac aaaaacaatg cagaattttg gcaaagtggc
     7141 cgtattgatg ggcggttttt ccagcgaacg agaaatctcg ctggacagcg gcaccgccat
     7201 tttgaatgct ttaaaaagca aaggcataga cgcatatgcc ttcgatccta aagaaacccc
     7261 attgtctgaa ttgaaggcac aaggttttca gacggcattc aacatccttc acggtactta
     7321 cggcgaagac ggggcggttc agggtgcatt ggaactgttg ggcattccct ataccggcag
     7381 cggtgtcgcc gcatccgcca tcggcatgga caaataccgc tgcaaactga tttggcaggc
     7441 attgggactg cccgttcccg agttcgccgt cctgcacgac gacactgatt tcgatgccgt
     7501 cgaagaaaaa ttgggcctgc cgatgtttgt gaaaccggcg gccgaaggca gcagcgtagg
     7561 cgtggtaaaa gtcaaaggaa aaggccgtct gaaaagcgtt tacgaagaat tgaaacacct
     7621 tcagggcgaa atcattgccg aacggtttat cggcggcggc gaatattcct gccccgtctt
     7681 gaacggcaaa ggcctgcccg gcatacacat catccccgcg accgagtttt atgactacga
     7741 agccaagtac aaccgcaacg acaccattta tcaatgtcct tcggaagatc tgaccgaagc
     7801 cgaagaaagc ctgatgcgcg aactggcggt tcgcggcgcg caggcaatcg gtgcggaaag
     7861 ctgcgtgcgc gtcgatttcc tcaaagatac cgacggcaaa ctctatctgt tggaaatcaa
     7921 caccctgccc ggtatgacga gccatagttt agtaccgaaa tccgctgccg ttacgggcgt
     7981 gggttttgcc gatttatgta ttgaaatttt gaagaccgca catgtgggat aatgccgaag
     8041 cgatggaacg gctgacgcgc tggctgcttg tcatgatggc gatgctgctt gctgcgtccg
     8101 ggctggtttg gttttacaat tcgaatcatc tgcccgtcaa gcaggtgtcg ctgaagggca
     8161 acctggttta ttccgataag aaggcattgg gcagtttggc gaaagaatac atccatggga
     8221 atattttgag gacggacatc aatggcgcac aggaggccta ccgccggtat ccgtggattg
     8281 cgtcggtcat ggtgcgccgc cgttttcccg acacggttga ggtcgtcctg accgagcgca
     8341 agccggtcgc gcgttggggc gaccatgcct tggtggacgg cgaaggcaat gtttttgaag
     8401 cccgcttgga cagacccgga atgccggtat tcagaggcgc ggaaggaacg tctgccgaaa
     8461 tgctccgccg ttatgacgaa ttttcgactg ttttggcaaa acagggtttg ggcatcaaag
     8521 agatgaccta tacggcacgt tcggcgtgga ttgtcgtttt ggacaacggc atcaccgtca
     8581 ggctcggacg ggaaaacgag atgaaacgcc tccggctttt taccgaagcg tggcagcatc
     8641 tgttgcgtaa aaataaaaat cggttatcct atgtggatat gaggtataag gacggatttt
     8701 cagtccgcta tgcttccgac ggtttacccg aaaaagaatc cgaagaatag tgggaacagg
     8761 tatcggacag attacgaccg tgccgtctga aacggtgcga cgcaaatttc aatcagtttt
     8821 aagagcagac gaacaatgga acagcagcaa agatacatca gcgtactgga tatcggtacg
     8881 tctaaagtcc tcgcactgat cggggaagtt caagatgacg acaaaatcaa catcgtcggt
     8941 ttggggcagg ctccttcacg gggcttgcgc gcgggcatgg taaccaatat cgatgccacc
     9001 gtccaagcca tcaggcaggc ggtcaatgat gccgagctga tggcggatac caaaattacc
     9061 cacgttacca caggtatcgc aggcaaccac atccgcagtc tcaattcgca aggtgtggtt
     9121 aaaattaaag acggggaagt cacgcaggca gacatcgacc gcgccattga aacggcaaag
     9181 gcaatcaata tcccgcccga tcaaaaaatt ctcgatgccg tggttcaaga ctacattatc
     9241 gacacccaac ttggcgtgag ggagcccatc ggtatgagcg gtgtgcgtct ggatacgcgg
     9301 gtgcacatca ttaccggtgc aagtacggca gtgcagaatg tccaaaaatg tatcgagcgg
     9361 tgcggtttga aaagcgatca gatcatgctt cagccgttgg caagcgggca ggcggtgctg
     9421 actgaagatg aaaaagacct cggcgtatgc gtcatcgaca ttggtggcgg aacgaccgat
     9481 attgccgttt atatgaacgg tgccatccgc catacgtccg tcattccggc cggtggtaat
     9541 ctgattacca aagatttgtc caaatcgttg agaacacctc tcgatgccgc cgagtacatt
     9601 aaaatccatt atggcgtggc atcatgcgat acggaaggct tgggtgagat gattgaagtt
     9661 ccgggcgtgg gtgaccggac atcgcgtcag gtttccagta aggttttggc agcaatcatc
     9721 agcgcacgta ttcaggagat ttttggcgta gtgctgggcg agctgcaaaa atcgggtttc
     9781 cccaaagaag tgctgaatgc gggtatcgtt ctgaccggcg gtgtgtccat gatgaccggg
     9841 attgtggaat ttgccgaaaa aatcttcgat ttgcctgtac gcaccggtgc gccccaagaa
     9901 atgggcggtt tgtccgaccg cgtccgcaca ccgcgctttt ctaccgccat cgggctgctt
     9961 catgcagcat gcaagctgga aggaaacttg ccgcagccgg aaaacggtgc agtgcaagag
    10021 agggaagggg gcggcggttt gttggcaaga ttgaaacggt ggattgaaaa cagcttctga
    10081 acaggtggat tgccgtttga caggtgagaa gtattttgcc agcagcaaga tacttcttat
    10141 ataatgaata ataatttatt taaaccgtcc tctgaatggg gcgagcagga gtttttgaat
    10201 ggaatttgtt tacgacgtgg cagaatcggc agtcagccct gcggtgatta aagtaatcgg
    10261 cttgggcggc ggcggttgca atgcaatcaa taacatggtt gccaacaatg tgcgcggtgt
    10321 ggagtttatc agtgccaata cggatgcgca gtctttggca aaaaaccatg cggcgaagag
    10381 aatccagttg ggtacgaatc tgacacgcgg tttgggcgcg ggcgcgaatc ccgatatcgg
    10441 ccgtgcggca gcccaggaag accgggaagc cattgaagaa gccattcgcg gtgcgaatat
    10501 gctgtttatc acgaccggta tgggcggcgg taccggtacc ggttccgcgc cggttgttgc
    10561 tgagattgcc aagtctttgg gcattctgac cgttgccgtg gttacccgac cgttcgcata
    10621 tgaaggtaag cgcgtccatg tcgcacaggc agggttggaa cagttgaaag aacacgtcga
    10681 ttcgctgatc atcatcccga acgacaaact gatgactgca ttgggtgaag acgtaacgat
    10741 gcgcgaagcc ttccgtgccg ccgacaatgt attgcgcgat gcggtcgcag gcatttccga
    10801 agtggtaact tgcccgagcg aaatcatcaa cctcgacttt gccgacgtga aaaccgtgat
    10861 gagcaaccgc ggtatcgcta tgatgggttc gggttatgcc caaggtatcg accgtgcgcg
    10921 tatggcgacc gaccaggcca tttccagtcc gctgctggac gatgtaacct tggacggagc
    10981 gcgcggtgtg ctggtcaata ttacgactgc tccgggttgc ttgaaaatgt ccgagttgtc
    11041 cgaagtcatg aaaatcgtca accaaagcgc gcatcccgat ttggaatgca aattcggtgc
    11101 ggctgaagac gagaccatga gcgaagatgc catccggatt accattatcg ctaccggtct
    11161 gaaagaaaaa ggcgcggtcg attttgttcc ggcaagggag gtagaagcgg ttgctccgtc
    11221 caaacaggag caaagccaca atgtcgaagg tatgatccgc accaatcgcg gtatccgcac
    11281 gatgaacctt accgctgcgg atttcgacaa tcagtccgta cttgacgact ttgaaatccc
    11341 tgcgattttg cgtcgtcaac acaattcaga caaataatgt gctgtttgcc cgtaaacctg
    11401 ctgcctcccg aatcggtttg tccggtttgg gaggtatgtt tttcaagatg ttgcaatttc
    11461 gtacggtttg cggtcggcgg attcagattt ttccacttga tacagacttt cagatatgga
    11521 cacttcaaaa caaacactgt tggacgggat ttttaagctg aaggcaaacg gtacgacggt
    11581 gcgtaccgag ttgatggcgg gtttgacaac ttttttgacg atgtgctaca tcgttatcgt
    11641 caaccctctg attttgggcg agaccggcat ggatatgggg gcggtattcg tcgctacctg
    11701 tatcgcgtct gccatcggct gttttgttat gggttttgtc ggcaactatc cgattgcact
    11761 cgcaccgggg atggggctga atgcctattt cacctttgcc gtcgttaagg gtatgggcgt
    11821 gccttggcag gttgcgttgg gtgcggtgtt catctccggt ctgattttta tcctgttcag
    11881 cttttttaaa gtcagggaaa tgctggtcaa cgcactgcct atgggtttga aaatgtcgat
    11941 tgctgccggt atcggtttgt ttttggcact gatttccctg aaaggcgcag gcattatcgt
    12001 tgccaatccg gcaaccttgg tcggtttggg cgatattcat cagccgtccg cgttgttggc
    12061 actgttcggt tttgctatgg tggtcgtatt gggacatttc cgcgttcaag gcgcaatcat
    12121 catcaccatc ttgaccatta ccgtcattgc cagcctgatg ggtttgaatg aatttcacgg
    12181 catcatcggc gaagtaccga gcattgcgcc gacttttatg cagatggatt ttgaaggcct
    12241 gtttaccgtc agcatggtca gtgtgatttt cgtcttcttc ttggtcgatc tatttgacag
    12301 taccggaacg ctggtcggca tatcccaccg tgccgggctg ctggtggacg gtaagctgcc
    12361 ccgcctgaaa cgcgcactgc ttgcagactc taccgccatt gtggcaggtg cggctttggg
    12421 tacttcttcc accacgcctt atgtggaaag cgcggcgggc gtatcggcag gcggacggac
    12481 cggcctgacg gcggttacag tcggcgtatt gatgctcgcc tgcctgatgt tttcaccttt
    12541 ggcgaaaagt gttcccgctt ttgccaccgc gcccgccctg ctttatgtcg gcacgcagat
    12601 gctccgcagt gcgagggata ttgattggga cgatatgacg gaagccgcac ccgcattcct
    12661 gaccattgtc ttcatgccgt ttacctattc gattgcagac ggcatcgcct tcggcttcat
    12721 cagctatgcc gtggttaaac ttttatgccg ccgcaccaaa gacgttccgc ctatggtatg
    12781 gattgttgcc gtattgtggg cactgaaatt ctggtatttg ggctgattga ttcgatatta
    12841 aaaatgccgt ctgaaaggtt ttcagacggc attttgtttg ccgatatatt taatttttat
    12901 taaattatat aaaaatcaaa tacataataa aatacatcgg attgcttaaa aataatacat
    12961 tgtttttatg tataaaatat tttataagtt ttcaggattt tgattatcga aaatttttct
    13021 tgatttcctg acaattttat tgaaacaaat aattcaaaat taatctagtt taatcatgga
    13081 attaaaataa aatattaaaa ttatgtaatg agtctcctta aaaatgtttg acattttcag
    13141 tcttgtgttt tagattatcg aaaaataaaa ctacataaca ctacaaagga acattactat
    13201 gaaaccaatt cagatgtttt ccccttttct gaataatccc cttgttttct tcttgtctgc
    13261 ggttttgccg cataattccg aacggtctgc tgtttttctt tgattcgttt taaatatcaa
    13321 taagataatt tttcccatat atttttaatg attggattgg gatgcccgac gcgtcggatg
    13381 gctgtgtttt gccgtccgaa tgtgatggaa gcctgtccat actgaaaaaa agtctataaa
    13441 ggagaaatat gatgagtcaa cactctgccg gagcacgttt ccgccaagcc gtgaaagaat
    13501 cgaatccgct tgccgtcgcc ggttgcgtca atgcttattt tgcacgattg gccacccaaa
    13561 gcggtttcaa agccatctat ctgtccggcg gcggcgtggc agcctgttct tgcggtatcc
    13621 ctgatttggg cattaccaca atggaagatg tgctgatcga cgcacgacgc attacggaca
    13681 acgtggatac gcctctgctg gtggacatcg atgtgggttg gggcggtgca ttcaatattg
    13741 cccgtaccat tcgcaacttt gaacgcgccg gtgttgcagc ggttcacatc gaagatcagg
    13801 tagcgcaaaa acgctgcggc caccgtccga acaaagccat tgtatctaaa gatgaaatgg
    13861 tcgaccgtat caaagctgcc gtagatgcgc gcgttgatga gaacttcgtg attatggcgc
    13921 gtaccgatgc gctggcggta gaaggtttgg atgccgctat cgaacgcgcc caagcttgtg
    13981 tcgaagccgg tgcggacatg attttccctg aagccatgac cgatttgaac atgtaccgcc
    14041 aatttgcaga tgcggtgaaa gtgcccgtgt tggcgaacat taccgagttt ggttccactc
    14101 cgctttatac ccaaagcgag ctggctgaaa acggcgtgtc gctggtgctg tatccgctgt
    14161 catcgttccg tgcagcaagc aaagccgctc tgaatgttta cgaagcgatt atgcgcgatg
    14221 gcactcaggc ggcggtggtg gacagtatgc aaacccgtgc cgagctgtac gagcatctga
    14281 actatcatgc cttcgagcaa aaactggata aattgtttca aaaatgattt accgctttca
    14341 gacggtcttt caacaaatcc gcatcggtcg tctgaaaacc cgaaacccat aaaaacacaa
    14401 aggagaaata ccatgactga aactactcaa accccgacct tcaaacctaa gaaatccgtt
    14461 gcgctttcag gcgttgcggc cggtaatacc gctttgtgta ccgttggccg taccggcaac
    14521 gatttgagct atcgcggtta cgacatcttg gatttggcac aaaaatgcga gtttgaagaa
    14581 gtcgcccacc tgctgattca cggtcatctg cccaacaaat tcgagctggc cgcttataaa
    14641 accaagctca aatccatgcg cggcctgcct atccgtgtga ttaaagtttt ggaaagcctg
    14701 cctgcacata cccatccgat ggacgtaatg cgtaccggcg tatccatgct gggctgcgtt
    14761 catcctgaac gtgaaagcca tccggaaagt gaagcgcgcg acatcgccga caaactgatc
    14821 gccagcctcg gcagcatcct cttgtactgg tatcaatatt cgcacaacgg caaacgcatt
    14881 gaggttgaaa gcgacgaaga gaccatcggc ggtcatttcc tgcaactgtt gcacggcaaa
    14941 cgcccaagcg aatcacacat caaagccatg cacgtttcac tgattctgta tgccgaacac
    15001 gagttcaacg cttctacctt taccgcccgc gtgatcgccg gtacaggctc tgatatgtac
    15061 tccagcatta ccggagcaat cggcgcgttg aaaggtccga aacacggcgg cgcgaacgaa
    15121 gtggcttacg atattcaaaa acgctaccgc aatgccgacg aagctgaagc cgacatccgc
    15181 gaacgcatcg gccgcaaaga aatcgtgatc ggtttcggtc atccggtgta taccatttcc
    15241 gacccccgca acgttgtcat taaagaagtg gcacgcggtt tgagcaaaga aaccggcgat
    15301 atgcgcctct ttgacattgc cgaacgtttg gaaagcgtga tgtgggaaga gaaaaaaatg
    15361 ttcccgaatc tggactggtt ctctgccgtt tcctaccaaa aattgggcgt accgaccgct
    15421 atgttcacac cgctgttcgt aatttcccgt acaaccggtt ggagcgcaca cgttcttgag
    15481 caacgcaaag acggcaaaat catccgtccg agcgcaaact acacgggccc tgaagatttg
    15541 gcgtttgtgg agattgaaga acgctgatga ttttcagacg accttgtttg tacaaggtcg
    15601 tctgaaaaaa gaaatttaat gaatttattg gcacactaat tattaatgcg gaacatttga
    15661 tggataagga caaggggtct tatgcgggta ttcctgtaca aaataccaaa gagttagttt
    15721 ggcagaaaat acaagacata cctatttctg aatgtgggga accgttggtc agtgttggta
    15781 ataccggacg cattcgtgtc aatcctgttt atttttctca gggtattgaa ggggcgataa
    15841 atagtgtttt attgcgtaga agtgttgctg ataggctgac tcaggttgtc tctttgctgc
    15901 cggaagtgta cgggttggaa atttttgatg gttggcgtcc tgtcatagtg caaagagcat
    15961 tacgagaaaa tatgaagcaa gaaattgaaa aacgccatcc ggaatatgat gaagttcaag
    16021 ttagagaagc actggactgt tttgctgctg atccagatcg ggcaggaatg cctctgcccc
    16081 atttgacagg cggatctgtt gatgtcggat tgtttgatat aaaaagtggc aaaagtttgg
    16141 atatgggaag tgagtttgat gcaactggac atttatctta tagcgatgct ttagagaaag
    16201 aaggtgagta tttctcctct gctcgtaatt tacgccggat tttgattcat gcgatgaaag
    16261 aggcaggatt tagtaatttg ccaaccgaat ggtggcattt cgattatggc aaccaaaatt
    16321 gggcttattt tacccaacaa agcaacgcag tttttggggc ggtagaagag caggatactt
    16381 aaattaaaat aaaaattggg aggaaaaatg caaactttaa tttcagcaga taatcatttg
    16441 ctgctgtggg cttttgttat gttagctgcc gctgctgcga tttttattga gcaaaacagt
    16501 cgcttggctg ggaaagtccc tggtgcagta ttagctttat tgattgcgtt gattgcttct
    16561 aatttgggtg ttattccgac ggatgctcca gtatttgatg ctgtatggtc ttatattgta
    16621 cctcttgcta ttccgttgtt attgtttcaa ttagatcttc atgctctatt taaggaaagt
    16681 ggacgtatgc tgtttatttt cctgattagt tcactaggga cggtcattgg aacgcttgtc
    16741 tcatttgcac tattgaaaaa ctatattcct gaactagata aggtagctgg catgatttca
    16801 gcatcttata cgggaggtgg tgttaatttt gcagctatgt cagctaagct taaccctgct
    16861 aaagatgtta ttgcagcaac aattgttgct gataatttga tgatggcttg ttactttttt
    16921 attttgattg gtatctcttc ctcatattgg gtcagaaaag tttggggaag tccatatcag
    16981 gataaattgg agcaggaata tcatgatccg agtgtgaata aggctgccga gtattggaaa
    17041 cctaaatcca tttcgctaca gagtatagcc ttatctttgg ctgcctcgat tttgttggtc
    17101 gcagtttcat tctcattatc cagttttttg caaaatcatt ttcaaagtgt tgatggtgtt
    17161 gcattaaaaa tgctgacagg tttaattagt gacaaatatt tgctgttaac gacatttaca
    17221 ttggcagttg ttttattttt ccgtaaaggc attcaaaaac tagatggcag tcaagaatta
    17281 gggacatatt gtatttatct gttttttgtt gtgattggtg ttcctgcctc tataccgtta
    17341 attattaaaa cagcaccatt actctttgtt tttactttga ttattgcggt attgaatttg
    17401 ttactgacgt tagtgttagg caagctgttc aaattcagta ttgaagaaat tgtattggct
    17461 tgtaatgcca atattggagg gccgactact gcagcagcct tagccatcag taaaggttgg
    17521 cgtgatttag tcggtgcaat tttgttaatt ggtactgtag gctacattat cggtaactat
    17581 ataggttcgt ttgtatatgc cctattgagc tgaagactaa tctaacaatc catgaacatt
    17641 ttcagacgac ccccttccaa aaacgtcggc tgaaacctca aaccacaaga aaaacagatc
    17701 cccaggagaa ccgacatggc tgccaaccaa cgttaccgca aaccgctgcc cggtacggat
    17761 ttggaatact acgacgcgcg tgcggcgtgt gaggacatca agcccggctc ttacgacaag
    17821 ctgccttata caagccgtat cttggctgag aacttggtca accgcgcgga caaagtcgat
    17881 ttgccgacgc tgcaaagctg gctgggccag ttgatagaag ggaagcagga aatcgacttt
    17941 ccgtggtatc ccgcgcgggt ggtgtgccac gatattctgg ggcagaccgc gctggtggat
    18001 ttggcgggct tgcgcgatgc gattgccgaa aaaggcggcg atcctgccaa agtgaatccg
    18061 gtggtgcaaa cccagcttat cgtcgaccac tctctggcgg tggagtgcgg cggttacgat
    18121 cctgatgcct tccgcaaaaa ccgcgaaatc gaagatcgac gcaacgaaga ccgtttccac
    18181 ttcatcaact ggacaaaaac cgcgtttgaa aatgtggacg tgattccggc gggcaacggc
    18241 atcatgcacc aaatcaatct agaaaaaatg tcgccggttg ttcaagttaa aaacggcgtg
    18301 gctttccccg atacctgcgt cggcacggat tcgcacacgc cgcacgtcga tgcgctgggc
    18361 gtgatttccg tgggcgtggg cggattggaa gcggaaaccg taatgctggg acgcgcgtcc
    18421 atgatgcgcc tgcccgatat tgtcggcgtt gagctgaacg gcaaacggaa ggcgggcatt
    18481 acggcgacgg atattgtgtt ggcactgacc gagtttctgc gcaaagaacg cgtggtcggg
    18541 gcgtttgtcg aattcttcgg cgagggcgcg agaagcctgt ctatcggcga ccgcgcgacc
    18601 atttccaaca tgacgccgga gttcggcgcg actgccgcga tgttcgctat tgatgagcaa
    18661 accattgatt atttgaaact gaccggacgc gacgacgcgc aggtgaaatt ggtggaaacc
    18721 tacgccaaaa ccgcaggctt gtgggcagat gccttgaaaa ccgccgttta tcctcgcgtt
    18781 ttgaaatttg atttgagcag cgtaacgcgc aatatggcag gtccaagtaa cccgcatgcc
    18841 cgttttgcca ccgtcgattt ggcggcgaaa gggctggcga agccttacga agagccttca
    18901 gacggacaaa tgcccgacgg cgcggtcatc atcgccgcga ttaccagttg caccaacact
    18961 tccaacccgc gcaacgtcgt tgccgccgcg ctcttggcgc gcaacgccaa ccgcttcggg
    19021 ctgaaacgca aaccgtgggt caaaacctcg tttgcccccg gttcgaaagt ggcggaaatt
    19081 tatttgaaag aagcaggcct gctgcccgaa atggaaaaac tcggcttcgg tatcgtcgcc
    19141 ttcgcctgca ccacctgcaa cggcatgagt ggcgcgctgg atccgaaaat ccagaaagaa
    19201 atcatcgacc gcgatttgta cgccaccgcc gtattatcag gcaaccgcaa cttcgacggc
    19261 cgtgtccatc cgtatgcgaa acaggctttc ctcgcttcgc ctccgttggt cgttgcctac
    19321 gcgctggcag gcagtatccg tttcgatatt gaaaacgacg tactcggcgt tgcagacggc
    19381 aaggaaatcc gcctgaaaga catttggcct gccgatgaag aaatcgatgc cgtcgttgcc
    19441 gaatatgtga aaccgcagca gttccgcgat gtgtatgtac cgatgttcga caccggcaca
    19501 gcgcaaaaag cacctagtcc gctgtacgat tggcgtccaa tgtccaccta tatccgccgc
    19561 ccaccttact gggaaggcgc attggcaggg gagcgcacat tgagcggtat gcgtccgctg
    19621 gcgattttgc ccgacaacat caccaccgac cacctctcgc catccaatgc gattttggca
    19681 agcagtgccg caggcgaata tttggcgaaa atgggtttgc ctgaagaaga cttcaactct
    19741 tacgcaaccc accgcggcga ccacttgacc gcccaacgcg caacctttgc caatccgaaa
    19801 ctgtttaacg aaatggtgag aaacgaagat ggcagtgtac gccaaggttc gctggcacgc
    19861 gttgaacccg aaggccaaac catgcgcatg tgggaagcca tcgaaaccta tatgaaccgc
    19921 aaacagccgc tcatcatcat tgccggtgcg gactacggtc aaggctcaag ccgcgactgg
    19981 gctgcaaaag gcgtacgcct cgccggtgtg gaagcgattg ttgccgaagg cttcgagcgt
    20041 atccaccgta ccaacttgat cggtatgggc gtattgccgc tgcagttcaa accgggtacc
    20101 aaccgccaca ccttgcaact ggacggtacg gaaacctacg acgtggtcgg tgaacgcaaa
    20161 ccgcgctgcg atctgaccct cgtgattcac cgtaaaaacg gcgaaaccgt cgaagttccc
    20221 gttacctgcc gcctcgatac tgcagaagaa gtattggtat atgaagccgg cggcgtgttg
    20281 caacggtttg cacaggattt tttggaaggg aacgcggctt agaggtcgtc tgaaaagcaa
    20341 gacgtagcgt gggtcgggtt caacattttg ctcattcacg taattctcga tatggcaggc
    20401 atctactgta aatcgtcatt cccgcgcagg cgggaatcca gaaagtggaa ttgaggaaac
    20461 cttatttatc cgatgagttt ctgtgcggac aaatttggat tcccgcctgc gcgggaatga
    20521 cggggtttaa taatctgccg tatcacaaca cagtagccgt agattgtggc gaaccccgac
    20581 agtttgcgga atcaaacggc tttggtcgga gtggcagcct aatccactat aaaaatcgtg
    20641 ggcagagccc acgctacata aggagaatct agaaatgccg caaattaaaa ttcccgccgt
    20701 ttactaccgt ggcggtacat caaaaggcgt gtttttcaaa cgttccgacc tgcccgaggc
    20761 ggcgcgggaa gcgggcagcg cacgcgacaa aatcctcttg cgcgtactcg gcagcccgga
    20821 tccctacggc aagcagatag acggtttggg caacgccagc tcgtccacca gcaaggcggt
    20881 gattttggac aagtccgaac gcgccgatca cgatgtcgat taccttttcg ggcaagtttc
    20941 catcgacaaa ccttttgtcg attggagtgg caactgcggc aacctcaccg ccgccgtggg
    21001 cgcatttgcc atcgagcaag gcttggtcga taaaggcaag attccttcag acggcatctg
    21061 cacagtcaaa atctggcaga aaaacatcgg caaaaccatt attgcccatg taccgatgca
    21121 aaacggcgca gttttggaaa caggcgattt tgagctcgac ggcgtaacgt tcccggcagc
    21181 cgaagtacaa atcgaatttc ttgatccagc cgacggcgaa ggcagtatgt tcccaaccgg
    21241 caatttggtc gatgaaattg atgtgccgaa tataggccgt ttgaaagcca cgctcatcaa
    21301 cgcgggcatt ccgaccgttt tcctgaatgc cgccgacttg ggctacacgg gcaaagagtt
    21361 gcaagacgac atcaacaacg atgccgcagc tttggaaaaa ttcgagaaaa tccgcgctta
    21421 cggtgcgctg aaaatgggtc taatcagcga cgtatccgaa gctgccgccc gcgcgcacac
    21481 gccgaaagtc gccttcgtcg cgcccgccgc cgattacacc gcctccagtg gcaaaaccgt
    21541 gaatgccgcc gacatcgatt tgctggtacg cgccctgagc atgggcaaat tgcaccacgc
    21601 gatgatgggt accgcctctg ttgccattgc gaccgccgcc gccgtgcccg gtacgctggt
    21661 caaccttgcc gcaggcggcg gaacgcgtaa agaagtgcgc ttcgggcatc cttccggcac
    21721 attgcgcgtc ggtgcagccg ccgaatgtca ggacggacaa tggacggcca ccaaagcggt
    21781 tatgagccgc agcgcacgcg tgatgatgga aggttgggtc agggtgccgg aagattgttt
    21841 ttaaattgac gtagcatggg tttgcccgcg agccataaac aggtcgtctg aaaaacaagt
    21901 aaacatcaaa tcactgacca ttcctttccc ttgccctgtg gcggaaggcg gcaaatcaca
    21961 aggaagaaca cggaaacccc gataaaagac agcttcccgt attaccgtca ttcccgcgca
    22021 ggcgggaatc cagacctgtc aatatggagg attggcaggg gaaaacaggt ttcgtgagtt
    22081 ctacattctg gattcccgcc acagcctgtc ctcgcgtagg cggggacgga ataacgatag
    22141 aaaatgcggc atatgctttg cccaaagagg ccgtctgaaa cacactccgc ccaacgccca
    22201 tcctttttca gacgacccca caccaaaaaa acaaccacaa actacaagga gaaacatcat
    22261 gtccgaccaa ctcattcttg ttctgaactg cggcagttca tcgctcaaag gcgccgttat
    22321 cgaccgcaaa agcggcagca tcgtcctaag ctgcctcggc gaacgcctga ccacgcccga
    22381 agccgtcatt acgttcagca aagacggcaa caaacgccaa gttcccctga gcggccgaaa
    22441 ttgccacgcc ggcgcggtgg gtatgctttt gaacgaactg gaaaaacacg gtctgcacga
    22501 ccgcatcaaa gccatcggcc accgcatcgc ccacggcggc gaaaaataca gcgagtctgt
    22561 tttgatcgac caggccgtaa tggacgaact caatgcctgc attccgcttg cgccgctgca
    22621 caaccccgcc aacatcagcg gcatcctcgc cgcgcaggaa catttccccg gtctgcccaa
    22681 tgtcggcgtg atggatactt cgttccacca aaccatgccg gagcgtgcct acacttatgc
    22741 cgtgccgcgc gagttgcgta aaaaatacgc tttccgccgc tacggtttcc acggcaccag
    22801 tatgcgttac gttgcccctg aagccgcacg catcttgggc aaacctctgg aagacatccg
    22861 catgattatt gcccacttag gcaacggcgc atccattacc gccatcaaaa acggcaaatc
    22921 cgtcgatacc agtatgggtt tcacgccgat cgaaggtttg gtaatgggta cacgttgcgg
    22981 cgacatcgat ccgggcgtat acagctatct gacttcccac gccgggatgg atgttgccca
    23041 agtggatgaa atgctgaaca aaaaatcagg tttgctcggt atttccgaac tttccaacga
    23101 ctgccgcacc ctcgaaatcg ccgccgacga aggccacgaa ggcgcgcgcc tcgccctcga
    23161 agtcatgacc taccgcctcg ccaaatacat cgcttcgatg gctgtggcct gcggcggcgt
    23221 tgacgcactc gtgttcaccg gcggtattgg cgaaaactca cgtaatatcc gagccaaaac
    23281 cgtttcctat cttgatttct tgggtctgca catcgacact aaagccaata tggacaaacg
    23341 ctacggcaat tcgggtatta tcagcccgac cgattcttct ccggctgttt tggttgtccc
    23401 gaccaatgaa gaactgatga ttgcctgcga cactgccgaa cttgccggca tcttgtagcc
    23461 aaaaaaggga cgagtccgca caatgccgtc tgaaattttc agacggcatt ttgtttggat
    23521 tttgggcgta caatacggaa aacatgacga taaggacaca aaccatggca cagtttttcg
    23581 ctattcatcc cgacaatccc caagaacgcc tcatcaagca ggcggttgaa atcgtcaatc
    23641 aaggcggcgt ggtggtttat ccgaccgatt cgtgttacgc cttgggttgc aaactcggcg
    23701 ataaggcggc gatggaacgc atactctcca tccgcaaaat cgatttgaaa caccacctga
    23761 ccctgatgtg cgccgatttg agcgagttgg gcacgtacgc caaaattgac aacgtacagt
    23821 ttcgtcagct taaagccgcc acgcccgggc cttatacctt tatcttgcag gcgacgaagg
    23881 aagtgccggc gcgcacgctg cacccgaaac gcaaaaccat cgggctgcgt attcccgata
    23941 atgccattgc acaagccctg ctgggggaat tgggcgagcc gcttttaagc tgcaccctga
    24001 tgctgcccga agacggcgaa ccattgaccg atccttatga aatccgcgag cgtttggaac
    24061 acgccgtcga tttggtgatt gacggcggct ggtgcggaac cgagccgacc accgtcgtcg
    24121 atatgaccga cggcacggaa ctggtgcgcc ggggttgtgg cgatacggcg gtgttcggtt
    24181 tgtagggaaa ccgatgccgt ctgaagcatc ggctgttcag acggcattgc gcgccttgcc
    24241 ggcggcagtc cgaaatgccg gcgcgtatcg cgctcggtcg gaatatccgt ttgaaacggc
    24301 attttgatgc attactgcac cgcaatcgga attctcggtt cgtagagcag gtcgtaggtc
    24361 ggcttgttga gcaggtcttg gagcgtgaaa ccgtccagat acgtgaaaaa cgacttcatt
    24421 gcaccgccga gtatgcctgt cagacggcag gacggtgtaa tcaggcattc gttgttcgaa
    24481 cccatacatt cgaccagctg catcggttcg aggtggcgga cgaccgagcc gatgttgatg
    24541 cggtcgggcg gtgtggcaag ccgcagaccg ccgccttttc cgcgcacgct gtggaggaag
    24601 ccgcctttga ccagcgcggt aacgaccttc atcagatggc ttttggaaat gccgtaggtt
    24661 acggcgatgg tactgatgtt gaccagcgca tcgtcgttga tggcagtgta gataaggacg
    24721 cgcagcccgt agtccgtatg ttgtgtcaaa tacatgattt tctcggtatg gattgttatt
    24781 cttatcggta cggtttaagg ttcacggaca ataccttaat ggttgaaacc ctgtccgtcg
    24841 gggcggtaga atgcagcctg tctgcggcgg tatgccgtct gaaacatccg cgctaccgtt
    24901 tgagaatttg ttattgtaac tcaaaatcat gaaaccgttg aaacgacatc ccgcccttat
    24961 cgggctttcg cgtgaccacc accattcgct ttccctgtgc gtgcgtctgt tgcggacgcc
    25021 ggaagaaagg catcgggacg aactcgaacc gcatttttcc gaattggaaa cccattttcg
    25081 cgaagaagaa accaagtttg ccccaatttg gcagaatgtc gcccccgaat tgaaacaacg
    25141 tttcgagaaa gaccacgccc gactgcggca gatgatggca agccccgaat acggtaacgc
    25201 ggcgtggaat accgcttttg ccacaaccct gcgcgaccac gcgcgctttg aagaacgcga
    25261 gctgtttccc gccgccgaac cgtttttgcc ggcatgattc cgttttgcgg caaatatatt
    25321 aatgataaac aaggaacaca catgaaattt accaagcacc ccgtctgggc aatggcgttc
    25381 cgcccgtttt attcgctggc ggctctgtac ggcgcattgt ccgtattgct gtggggtttc
    25441 ggctacacgg gaacgcacga gctgtccggt ttctattggc acgcgcatga gatgatttgg
    25501 ggttatgccg gactggtcgt cgtcgccttc ctgctgaccg ccgtcgccac ttggacgggg
    25561 cagccgccca cgcggggcgg cgttctggtc ggcttgacta tcttttggct ggctgcgcgg
    25621 attgccgcct ttatcccggg ttggggtgcg tcggcaagcg gcatactcgg tacgctgttt
    25681 ttctggtacg gcgcggtgtg catggctttg cccgttatcc gttcacagaa tcaacgcaac
    25741 tatgttgccg tgttcgcgct gttcgtcttg ggcggcacgc atgcggcgtt ccacgtccag
    25801 ctgcacaacg gcaacctagg cggactcttg agcggattac agtcgggctt ggtgatggtg
    25861 tcgggtttta tcggtctgat tggtacgcgg attatttcgt tttttacgtc caaacggttg
    25921 aatgtgccgc agattcccag tccgaaatgg gtggcgcagg cttcgctgtg gctgcccatg
    25981 ctgaccgcca tgctgatggc gcacggcgtg atgccttggc tgtcggcggc tttcgcgttt
    26041 gcggcaggcg tgatctttac cgtgcaggtg taccgctggt ggtataagcc tgtgttgaaa
    26101 gagccgatgc tgtggattct gtttgccggc tatctgttta ccggattggg gctgattgcg
    26161 gtcggcgcgt cttatttcaa acccgctttc ctcaatctgg gtgtgcatct gatcggggtc
    26221 ggcggtatcg gcgtgctgac tttgggcatg atggcgcgta ccgcgctcgg tcatacgggc
    26281 aatccgattt atccgccgcc caaagccgtt cccgttgcgt tttggctgat gatggcggca
    26341 accgccgtcc gtatggttgc cgtattttct tccggcactg cctacacgca cagcatacgc
    26401 acctcttcgg ttttgtttgc actcgcgctt ttggtgtatg cgtggaagta tattccttgg
    26461 ctgattcgtc cgcgttcgga cggcaggccc ggttgagaca aaccgccgca gatttcgggt
    26521 ctgggcggtt tgcttttcag acggcagggc ggtcagttgc cgtccagcca gcggtcgcgt
    26581 gtggttttgg cttcttcaaa atagcggtac agggcttcgc ggtcgtcggt ggtcaggatg
    26641 tttgccaaaa cgtccaactg tttgcccaag ccttgaacca gttgcagcag gctgtctttg
    26701 ttggcaaggc agatgtccgc ccacacggcg ggatggccgg aggcgatgcg ggtgaagtcc
    26761 cgaaagcccg tggcggcgaa tttcagatat tcctgtccgt cggggtggtc gagaatctgg
    26821 tggacatagg cgaaggcggt caggtggggc atatgggaga cggcggcgaa aaccgcgtcg
    26881 tggcgttgcg cgtccatcgt ataaatttcc gcaccgaccg cgtgccacag ttcttctacc
    26941 aaggcaatgc cgtctgaatg ttcgccgccg tgtggcgtga tgatgagttt tctgtggcgg
    27001 aacagcccga actgcgcggc ttgcgcaccg cttctgtccg aaccggcaat cgggtgggcg
    27061 gcgatgcagt ggtgcagacg gtcgggcaga cagcggcgga aggcttcgat gaccgaagat
    27121 ttggtgctgc cgacatcgga aatccaagtg tgttccggca aaacggggcg cagcgcggtc
    27181 aaaatggcgg gaacggtggc gacgggcgtg gcaatcagta ccaagtccgc accgccgatg
    27241 ctgtccgcgt cgatggcaac ggaagcccgg tcaatcacgc cgcgttccaa tgcacgttcg
    27301 aggttgtcgc ggtcggtgtc gataccggta acggtgcgga cgagtccctg ccttttgagg
    27361 tcgagaacga acgaaccgcc gatcagccct acaccgatga gggcaatatg gttcaaaatg
    27421 ggcatttgtg taaacggttt tcgcaaagta ccgtcatggt agcctatcgg cggaatatgc
    27481 cgcaaggtcg gcaggaaaaa ggagaagaaa tggacaaaat cagagttgcc gccgtgcaga
    27541 tggtgtcggg cgtgtcgccg gaaaccaacg tcgctgccat gaaacgcctg gtcgcacggg
    27601 cggcggagca gggggcggat tgggtgctgc tgcccgaata ttgggtgctg atgggcgcaa
    27661 acgataccga caaactcgcg cttgccgagc ctttgggcgg cggacgcttt cagacggcat
    27721 tgagcgaaac ggcgaaagaa tgcggcgtgg tgctgttcgg cggaaccgtg ccgctgcaaa
    27781 gccccgaggc gggcaaggtc atgaatactt tattggtgta cgggcgcgac ggcaaacgga
    27841 cggggctgta ccacaaaatg cacctcttcg gcttttccgg tttgggcgaa cgctatgccg
    27901 aagccgatac catcagcgca gggcgggcag tgccgcactt gtcggcagag ggcgtgccgg
    27961 tggcggcagg tatttgttac gatgtccgct ttcccgaatt tttccgccgc cagctgccgt
    28021 ttgacgtatt gatgcttccg gcggcattta cccatacgac gggcaaggcg cactgggagc
    28081 tgcttttgcg tacgcgtgcc gtcgaaaacc aatgttatgt tgtggcggcg gcacagggcg
    28141 gattgcacga aaacggcagg cgcacgttcg gacacagtat gattgtcgat ccgtggggcg
    28201 acgtgttgga cgtattgccc gaaggcgaag gcatcgttac ggcagacatt gatgccaacc
    28261 gcctgaacag cgtccgcaac cgcctgcccg ccctgaaata ccgggttttg gatgccgtct
    28321 gaaggttcag acggcatcgg tgccggggaa tcagcgggct gtaggatggg cttcagcctg
    28381 ccatttccat caatcccaca tttctaccgt tttcatcgaa tccatcgaat ccgccccttt
    28441 cgacgaccca gtcc
//
LOCUS       DAWEWP010000007        26525 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWEWP010000007 DAWEWP010000000
VERSION     DAWEWP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26525
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.007"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            124..2151
                     /gene="uvrB"
                     /locus_tag="WGN53_000199"
     CDS             124..2151
                     /gene="uvrB"
                     /locus_tag="WGN53_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ0139239.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDGGKGRRQGKNKVKV
                     SEIHNEEDAVKEIAKLEKAMQQAARDLQFEEAAVLRDRIRGIKEGLLFGSE"
     gene            2263..2550
                     /locus_tag="WGN53_000200"
     CDS             2263..2550
                     /locus_tag="WGN53_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139240.1"
                     /translation="MTYSFRVENINDISDIPENHLEIVRAALPSNRSNALSREPDGAQ
                     AVFVYEIDGEFRAKLFDRIKDTDSYLQATEDNYSNDPRSIGVVYVLEQQQG"
     gene            complement(2731..3447)
                     /gene="trmB"
                     /locus_tag="WGN53_000201"
     CDS             complement(2731..3447)
                     /gene="trmB"
                     /locus_tag="WGN53_000201"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ0139241.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVIENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYARQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRMG"
     gene            complement(3569..4633)
                     /locus_tag="WGN53_000202"
     CDS             complement(3569..4633)
                     /locus_tag="WGN53_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0139242.1"
                     /translation="MYLLYASRHFEEALEWAKTSAKNNNPHGQYLLAQYCRYGTPPDF
                     ETAHLLYRKAAAQGLPEAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPL
                     AELILPTAPDEAVYRFQQAAQENDPDAHAALADIYLQGKYQERNHKLALHHAEAAAAE
                     RHPEGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPDQYG
                     GIKDSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMY
                     YFGQGIAADYGKARKWFEQAAAQKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAIN
                     NGYGQKSVLQELLQQWQNAV"
     gene            complement(4698..4973)
                     /locus_tag="WGN53_000203"
     CDS             complement(4698..4973)
                     /locus_tag="WGN53_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139243.1"
                     /translation="MNNPDLPYRQTLECLSQKQYNFTEVRRLLTEAALAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPP"
     gene            complement(5090..6976)
                     /gene="uvrC"
                     /locus_tag="WGN53_000204"
     CDS             complement(5090..6976)
                     /gene="uvrC"
                     /locus_tag="WGN53_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ0139244.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPDPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPHSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(7031..9193)
                     /locus_tag="WGN53_000205"
     CDS             complement(7031..9193)
                     /locus_tag="WGN53_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ0139245.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQAEAEHHIGWRLWLLSAINIPFLVG
                     MAGMMIGRHDWMIPPLWQFALASIVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRDGEWKQLPIDQVQIGDLIRANHGERIAADGVIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDKDDLYRI
                     AAAVEQNAAHPLARAIVSATQARGLEIPAAQNAQTVVGAGIAAEVEGAGLVKAGKAEF
                     AELTLPEFSDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAQQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            9332..10282
                     /gene="trxB"
                     /locus_tag="WGN53_000206"
     CDS             9332..10282
                     /gene="trxB"
                     /locus_tag="WGN53_000206"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ0139246.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            10437..10805
                     /gene="rpsF"
                     /locus_tag="WGN53_000207"
     CDS             10437..10805
                     /gene="rpsF"
                     /locus_tag="WGN53_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ0139247.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            10806..11108
                     /gene="priB"
                     /locus_tag="WGN53_000208"
     CDS             10806..11108
                     /gene="priB"
                     /locus_tag="WGN53_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ0139248.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            11115..11345
                     /locus_tag="WGN53_000209"
     CDS             11115..11345
                     /locus_tag="WGN53_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ0139249.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            11362..11814
                     /locus_tag="WGN53_000210"
     CDS             11362..11814
                     /locus_tag="WGN53_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ0139250.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEVRRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            11828..11935
                     /locus_tag="WGN53_000211"
     CDS             11828..11935
                     /locus_tag="WGN53_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ0139251.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKVGMVVFIVD"
     gene            complement(12602..13408)
                     /locus_tag="WGN53_000212"
     CDS             complement(12602..13408)
                     /locus_tag="WGN53_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0139252.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKRAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPKLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(13409..14155)
                     /gene="pssA"
                     /locus_tag="WGN53_000213"
     CDS             complement(13409..14155)
                     /gene="pssA"
                     /locus_tag="WGN53_000213"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ0139253.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            14738..15220
                     /locus_tag="WGN53_000214"
     CDS             14738..15220
                     /locus_tag="WGN53_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ0139254.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(15272..16483)
                     /locus_tag="WGN53_000215"
     CDS             complement(15272..16483)
                     /locus_tag="WGN53_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ0139255.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            16706..19144
                     /locus_tag="WGN53_000216"
     CDS             16706..19144
                     /locus_tag="WGN53_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ0139256.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDSPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            19353..20663
                     /locus_tag="WGN53_000217"
     CDS             19353..20663
                     /locus_tag="WGN53_000217"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ0139257.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NSDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDIFKEQAERRVSLGLILAKLVDENKLEPTEEQVKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            20759..21373
                     /gene="clpP"
                     /locus_tag="WGN53_000218"
     CDS             20759..21373
                     /gene="clpP"
                     /locus_tag="WGN53_000218"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ0139258.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(21430..22695)
                     /gene="ispG"
                     /locus_tag="WGN53_000219"
                     /gene_synonym="gcpE"
     CDS             complement(21430..22695)
                     /gene="ispG"
                     /locus_tag="WGN53_000219"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ0139259.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKITLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(22711..23472)
                     /gene="pilW"
                     /locus_tag="WGN53_000220"
     CDS             complement(22711..23472)
                     /gene="pilW"
                     /locus_tag="WGN53_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ0139260.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(23475..24569)
                     /gene="rlmN"
                     /locus_tag="WGN53_000221"
     CDS             complement(23475..24569)
                     /gene="rlmN"
                     /locus_tag="WGN53_000221"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ0139261.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(24712..25137)
                     /gene="ndk"
                     /locus_tag="WGN53_000222"
     CDS             complement(24712..25137)
                     /gene="ndk"
                     /locus_tag="WGN53_000222"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ0139262.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(25196..26347)
                     /locus_tag="WGN53_000223"
     CDS             complement(25196..26347)
                     /locus_tag="WGN53_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ0139263.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         6292 a         6629 c         7222 g         6382 t
ORIGIN      
        1 ttgggtagcg attgggtttc agacggcata cattcaggat ttcaccgcct tatgccgtct
       61 gaaagacgtt tccgacccat ccctactaca cgctttgtcc gcaaacgcat cacaggcaag
      121 attatggaag tcatccgata ccccgattcc cctttcaaac tccaccaacc cttcccgccc
      181 gcaggcgacc agcctaccgc tattgccggc ttgctcgaag ggctttcaga cggccttgcc
      241 taccaaaccc tgctcggcgt aaccggttcg ggcaaaacct acaccatggc gaacgtcatc
      301 gcccaaagcg gccgacccgc catcatcatg gcgcacaaca aaacccttgc cgcccagctt
      361 tatgccgaaa tgcgcgagtt tttccctgaa aacgcggtgg aatatttcgt ctcctactac
      421 gactattacc aacccgaagc ctatgtgccc agccgcgacc tgttcattga aaaagacagc
      481 gcgatcaacg aacatatcga gcagatgcgc ctttccgcca ccaagaacct gatgacgcgc
      541 gacgacgtga ttatcgtcgc caccgtgtcc gccatctacg gtatcggcga cccgaccgag
      601 tatcagcaaa tggtgttgtc cgttaaagaa ggcgacacca ttgagcagcg cgacatcatc
      661 gccacgctcg tttccatgca gtacgagcgc ggcgatttgg actttaaacg cggcagcttc
      721 cgcgtgcgtg gcgacgtgat tgacgtgtac cccgccgaaa gctccgaaaa cgccttgcgc
      781 atcagcctgt ttgacgacga aatcgaccgc ctcgatatgt tcgacccgct ttcaggcagc
      841 cttatccagc gcgtcggccg ctacaccgtc ttcccgtcca gccattacgt taccccgcgc
      901 gataccgtct tgcgcgcctg cgagtccatc aaagaagaat tacgtgaacg cattgatttc
      961 tttaccaaag aaaaccgtcc cgtcgaacaa caacgcatcg aacagcgcac ccgctttgac
     1021 ctcgaaatgc tctacgaaat gggcttctgc aaaggcatcg aaaattactc ccggcacttc
     1081 tccggcaaaa aagaaggcga accgccgccc acgctgatgg actacctgcc tgacaacgcc
     1141 atcatgttca tcgacgaaag ccacgttacc gtcacccaaa tcggcggcat gtacaaaggc
     1201 gacgcatcgc gcaagcaaaa cctcgtcgac tacggcttcc gcctgccttc cgcccgcgac
     1261 aaccgcccgc tcaaattcca tgaatttgaa aaagtcatgc cgcaaactgt cttcgtttcc
     1321 gccactccag ccaaatacga agaagaacac gccggacaag tggtcgaaca agtcgtccgc
     1381 cccacagggc tggtcgaccc ccaaatcatc atccgccccg tcgccaccca agtcgacgac
     1441 ttaatgagcg aaatcaacga ccgcatccaa aaaggcgaac gcgttctcgt taccaccctc
     1501 accaaacgca tggcggagca actcaccgac tattacagcg aactcggcat caaagtgcgc
     1561 tacctgcaca gcgacattga taccgtagag cgcgttgaaa tcattagaga tttacggctc
     1621 ggcctgtttg acgtactcgt cggcatcaac ctcttgcgcg aaggcctcga tatccccgaa
     1681 gtctccctcg tcgccatcct cgacgccgac aaagaaggct tcctgcgctc acaccgcagc
     1741 ctgattcaaa ccataggccg cgccgcgcgc aacgtgaacg gcgtcgcgat tctgtacgcc
     1801 gacaaaatca ccgactctat gaaagccgcc atcgacgaaa ccgaacgccg ccgtgaaaaa
     1861 caaattaaat tcaacgaaga acacggcatc gtgccgcaac agattaaaaa acaggtcaaa
     1921 gacatcattg acggcgtgta ccacgaagaa gacggtggta aaggccgtag acaaggcaaa
     1981 aacaaggtta aagtcagcga aattcacaac gaagaagacg cggttaaaga aatcgccaaa
     2041 ctggaaaaag ccatgcagca ggcagctagg gatttgcagt ttgaagaggc ggctgtactg
     2101 agggatagga tacgggggat taaggaaggg ttgttgtttg ggagtgagta ataaaagcac
     2161 ttgaaagttt agttgatgac cacaatgttc ttaaaagatc tggagaaaca aaagatgtgg
     2221 agaaacatcg taacttgatc aattacattc tgaggactga aaatgacata ttcttttcgt
     2281 gtagaaaata taaatgatat atctgatatt cctgaaaatc atttggaaat agttagagct
     2341 gctctgccta gtaatagaag taatgcctta tctcgtgagc cagatggggc acaagctgtt
     2401 tttgtttatg aaattgatgg tgaatttaga gctaagttat ttgaccgcat aaaagataca
     2461 gacagttatt tacaagcaac agaggataat tactctaatg accctcgcag tataggagta
     2521 gtatacgttt tggagcaaca gcaagggtag cctgagggcc gcacaaactc tcaagctgac
     2581 gtgcagtatg tgtgatacgc acgcatgcgt tttttcgttc aaaccattgt tttaaacctt
     2641 gtatcgaaac atcactcgct gctttatctt gaaaaaatgc cgtctgtaag caaacatgct
     2701 tcaaacagca tttttttatc cggcggtttg ttagcccatc cgtttgaata ccaagtccca
     2761 aacgccgtgc ccgaggcgtt tgccgcgcgc ttcgaatttg gtttcggggc ggtagtccgg
     2821 ggtgggggcg tagtctgccg ccgtattttg caggctgtcg aagctactga ggacttcaag
     2881 catctgccgt gcatattctt cccagtctgt cgccaggtgg atatagccgc cggttttgag
     2941 tttgggcagc agtttggcga tgaagggggc ttgtatcaga cggcgtttgt ggtggcgttt
     3001 tttgtgccac gggtcgggga agaatatgtg gatgccgtcg agcgagccgt cttgcagcat
     3061 attttcgata acttctacgg catcgtgccg catcacgcgg atgttttcca aatggttttc
     3121 gttgatgagt ttgagcagat tgcctacgcc gggaccgtgt acgtcgatgg cgaggaagtc
     3181 ggtttcgggc aggcggcggg cgatttctgc ggttgccgtc cccataccga agcctatttc
     3241 gaggattttg ggtcggttgc ttccgaaatt ggcatcaaga tcggccggtg ttgcttggta
     3301 atcgatgccg aagctgtccc ataaggtatc gatggcgcgt tgctgtgcgg cggtcatatg
     3361 gctttggcgg aggacgaagc tgcggatgct gcggggatgt tcggcttgcc agttgttttg
     3421 tgtttggttt tcggcggtgt ctgtcataat gcttccgtgc gcttgtgcaa tcaggatgcc
     3481 gtctgaatgt gaaaggcctg ttcggacggc gggaagggaa aggggcgatt atacctgttt
     3541 cgtttcggca gggtaggtgt aacgctgttc agacggcatt ttgccattgt tgcagcagtt
     3601 cttgcaggac gcttttttgc ccgtatccgt tgtttatggc ttcttgcagg tagcggcagg
     3661 ctttttcttt gtccggcccg acaccgtgtc cgctgtaatg gatgcaggcg aggttgtaga
     3721 acgccatact gtctttttgc gcggcggctt gttcaaacca tttgcgggct ttgccgtagt
     3781 cggcggctat gccctgtccg aagtagtaca tactgcccag attggtttgg gctttgctgt
     3841 gtccgagttc tgcggcttct gtgtagagtt tgagcgcggc tgcgtattcg ggcgcacatt
     3901 gtaatccgta atgcagggct tgggcttttt gataaagctg ttctgccctt tgccgcctga
     3961 tggcggaatc tttgatgccg ccgtattggt cgggatggtt taacgcgctg tcggatatga
     4021 gtttctgata ggcggaaagg ctgccggctt cggctgcctg ccggtaataa tgccgggctt
     4081 tttccgtatc gggggctatg cccaaaccgt agcggcagat gtcgcccagt atccgcaaac
     4141 cttcgggatg gcgttctgct gcggctgctt cggcatgatg cagggcaagt ttgtggtttc
     4201 tttcctgata tttgccttgt aggtagatgt cggccagtgc ggcatgggca tcggggtcat
     4261 tttcctgtgc cgcctgttgg aaccggtaaa cggcttcatc gggagccgta ggtaggatga
     4321 gttcggcaag cggggtgtag gcgggaatgt atccttgttg cgcggcggcg cgcaaatgat
     4381 tgacggcctg tgccgtgtcg actttcgtcc cttgcccgaa acggtattgc agcccgagct
     4441 gccaatgtgc ttccggcaag ccttgtgccg ccgcttttcg gtagagcagg tgcgccgttt
     4501 caaaatccgg cggtgtgccg taccggcagt attgggcgag cagatattgt ccgtgggggt
     4561 tgttgttttt tgcgctggtt tttgcccatt cgagtgcttc ttcaaaatgc cgggacgcgt
     4621 acagcaggta catcaggcgg acttgcgctt cgggcactcc tgcttctgcc ggaggcctgt
     4681 aaagcgatat cagggtttca ggggggcgcg ccttccaatt cttggatata tgccagattg
     4741 taacgcgcgt agggatgccc ctgttcggcg gcgatgcgga gcatttccat accttgttcg
     4801 cggtcttggt aggggctgtc cgcatccatc aggtgttttg ccaactcgaa tgcggcggcg
     4861 ggatgacctg ccaaggccgc ttctgtcagc agtcggcgga cttcggtaaa gttatattgt
     4921 ttttgagaaa ggcattctaa ggtctgccga tagggtaagt cgggattgtt catatcagat
     4981 cgttttccgt tttgtcgaat catcgttata tgttatgaag ttaggtttat atcggcacgc
     5041 gggcctgctg tatcatattt ttacagacgg tttttgtttt ggcggcatac taatgcagat
     5101 gattataaat ggtttccgcc aatgccttgc tgatgccttc cactttttcc aagtcctcgc
     5161 ggctggcggc aatcacgccg cgcaggccgc cgaagcgggt gagtagggct tggcggcgtt
     5221 tgctgcccac gccggggatg tcgctgaggg acgaggtaac gcgtgctttg tcgcgttttt
     5281 tgcggtggcc tgtaatggca aagcggtgtg attcgtcgcg cacggtttgc agaaggtgca
     5341 gggcggggct gtgcgggggc aggcggaaga cttcgccggt aaaaggcagt atgagctcct
     5401 ccataccggc tttgcgctcc gggcctttgg cgataccgac caaaggaatg tgcagcccga
     5461 gttcttccca taccgatacg gctacgccga tttgcccttt gccgccgtca attaacacgg
     5521 catccggcca tttgacggtc tcgccgttgg cttcggcctc ctgcattttg ccgtaacggc
     5581 gcgtcaacac ttcgcgcatg gcggcgtagt cgtcgccggg tttggcggtc gtgatgttgt
     5641 agcggcggta ttgcgaaggc tggatgtttt gctcatcgta cacaacgcag gacgcaatag
     5701 tggcttcgcc ttgtgtgtgg ctgatgtcga aacattcgag gcggttgagg ccgtctgaat
     5761 ccataccgag gattttggcg agttcgtcga tgcggtgttg ctggctgctt tgttgcaggc
     5821 ggcgttgtgc aatcgccatt tgtgcgtttt gttccgccat tttcaaccag actttgcgtt
     5881 cgcctatggt tttggtgaca aattgcatct gcttgccgtg ttcgccttcc aaagcctctt
     5941 tcagcgcatc gggaacggaa aagttgctaa taatgatgtc gggtttgctt ttgcccaaat
     6001 aatgttgggc gacgaaggct tcggcgtaat cttgtccgtt tggatcggga tcgtttttgg
     6061 tgtcggggaa aaagcttttg tcgccgacgt gccgcccacc gcgtatgctg acccagtgta
     6121 cgcagacgat gccgtctgaa accgccagcg cgagcaaatc gatatcgttg gggttgttcg
     6181 ggtttttgct gtcgatgaac tggttgctct gcataatgcc gagtgcttgg atttggtcgc
     6241 ggtagcgggc ggcttcttca aattgcagat tcgcggcggc agtttgcatt ttgtgttgca
     6301 gggtgcgggt cagttcgtcg gttttgccgt tgagaaaagt ggcggcttga cgcacgctgt
     6361 cgcggtagtc ttcttcgctg atgtgtccga cgcagggcgc ggtacagcgt ttgatttggt
     6421 acagcaggca agggcggtcg cggtgttcga acacgctgtc ttcgcaggta cgcagcataa
     6481 agactttttg caggacttgg atgctgtcgc gcacggcgtt gctgttggga tacgggccga
     6541 agtattggtt gggctttttc agcgtgccgc ggtaatacgc catttgcgga tattgatggc
     6601 cgctgagcat cagataagga tagcttttgt cgtcgcggaa aagaatattg tatttgggcg
     6661 acagggcttt gatgaagttg ttttcgagaa tcagggcttc ggattcggag cgggtgatgg
     6721 tggtttcgat gtggcgaacc tgttttacca tcaatgcgat gcgcggcgaa tggtcgtttt
     6781 tttggaaata gccggacacg cgccgcttga ggttgaccgc tttgccgacg tataagactt
     6841 tgccgccttc gtcaaaaaaa cggtacacgc ccggcagctt gggcaggttt ttgaggaaga
     6901 gcgggatgtc gaattgttcc ggagaacggt tttctgtatt catatcaatc atttgaaaaa
     6961 cggcttcaga cggcatatcg gcagcggcag ccttatccgt tcggaaaagc tgtcgggtgg
     7021 ttatatgcgg tcaatcgatt tttacccgtt tcaggcgcaa ggcattgctc aacacggaaa
     7081 ccgagcttgc cgccatggcc gcgcctgcga tgacgggatt taaaaagcca agcgcggcga
     7141 gcggaatgcc caagatattg tagaagaagg cgaaaaacag gttttgcttg atgtttttca
     7201 aagtggcttg cgacaccagc agggcatcgg cgagctgatt aaccgaatgc tgcatcagcg
     7261 tggcggacgc ggtgtgttcg gcaacgtccg caccgccttt catggcgaag ctgacgtttg
     7321 cggcggcgag cgcgggcgcg tcgttgatgc cgtcgccgac catcgccacg gttttgccgg
     7381 cggctttgag tttctgcact tcggcggctt tgtcgcgcgg actcatattg ccgaaggcgt
     7441 gagcgatacc cagttgttgg gcgacgtatt cgaccgtgcc ttggttgtcg ccgctcatga
     7501 tgtaaacatc gatattgtgt tttttcagac ggcctatggc ttcggcggta tcggctttca
     7561 acgcgtcggc aagtgcgaat gcaccgatag gtttgttatc gactgagact gcaacaatgc
     7621 ttgcaatgtc ccaaacgccg tctgagaact cgggcaaggt cagttcggca aattcggctt
     7681 tgcccgcttt caccaatccc gcaccttcca cttcggcggc aatgcctgcg ccgacaacgg
     7741 tttgcgcgtt ttgtgcggcg ggaatctcca aaccgcgcgc ttgggtggcg gaaacgatgg
     7801 ctcgggcaag cggatgggcg gcgttttgtt cgacggcagc ggcgatgcgg tacaaatcgt
     7861 ctttgtcaaa gccgctgtcg ggaacacaat aaacggcggc aacctgcggc ctgccttcgg
     7921 tcagcgtgcc agttttgtcc aacacaacgg catcgacgtg ggcggcttct tccattgccg
     7981 ctgcgtcttt aaaccaaatg ccgtgtttca ctgctttacc catgccgacc ataatcgcgg
     8041 cgggtgtagc cagaccgagc gcacacgggc aggcaatcac caaaacggcg acggcgtgca
     8101 tcagcgcaac cgtccaatcg cccttaatca gccaagtaac aataaaagtc aacaacgcga
     8161 tgcccacgac ggcaggcacg aacaccgccg ccgccttgtc cgccacgcgc gcaatcggtg
     8221 ctttactgcc ttgcgcttca gagagtgcgt tcatcatgtc gccgagcagg gtttggctgc
     8281 cgagctgcgc ggcgcggtac accacgctgc cttcggtcat cagcgcgccc gccaacactt
     8341 tgccgcccgc ctttttctct tcgggattgg attcgccggt aagatggctc tcgtccgccc
     8401 agccgctgcc gctttcgata acgccgtcgg cggcaatgcg ttcgccgtgg ttggcgcgga
     8461 taaggtcgcc gatttgcact tggtcgatgg gaagctgttt ccattcgcca tcgcgctgca
     8521 cgttgacttg ggtcggcgtg agtttgagca gcaagcccaa gctgttcagg ctggattttt
     8581 tggtacggtg ttccaaaaat ttgcccagcg acacaaaacc aatcaccatc acgcccgctt
     8641 caaaatacac gtgcgccatt ccgtgtgccg cgtgcggact gaaaaacaac atataaacgg
     8701 aatacaggta aatcgacacc gtgccgatag taaccagcac gtccatattc gccagcccgc
     8761 ctttaatgct cgcccacgcg cttttgtaaa acgggattgc cagccaaagt tgcacaatgc
     8821 ttgccagcgc aaactgccac aacggcggaa tcatccaatc gtgcctaccg atcatcatcc
     8881 ccgccatacc gacaaggaac ggaatattga tggcggacag cagccacagc cgccagccga
     8941 tatggtgttc tgcttcagct tgcggcaatg tatcttccgt tttttccttc gcgccgtaac
     9001 cggttttctc aatgattttg gcaatgtcgg ctactgaggt tttgctgtcg tcaaacacta
     9061 cctgcgcctc ttcgctggcg aagtttaccc ccgccgattc gacaaaatct tttttgttca
     9121 acactttttc aatgcgcgaa gcgcaggcct ggcaggtcat gccttcgatt tggaaacgga
     9181 ttttttgttg cataagcgtg attcctgctt tgatattcag acggcagtta aggctgcggg
     9241 atgccgtctg aacgccggta tggtcgaaat ttctcaaacg cgccgataat tcgagtagaa
     9301 tccgaaaact tgacacagca aaggaaaaac aatgtcccaa caccgcaaac tgattatttt
     9361 gggttccggc cccgccggat acaccgccgc cgtctatgcc gcgcgcgcca atttaaaccc
     9421 cgtcattatt acaggtatcg cgcaaggcgg gcaactgatg accaccaccg aagtggacaa
     9481 ctggcctgcc gatgccgacg gcgtgcaagg gccggaattg atggcgcggt ttctcgccca
     9541 cgccgaacgt ttcggaacgg aaatcatttt tgaccaaatc aacgccgtcg atctgcaaaa
     9601 acgcccgttt acactcaaag gcgatatggg cgagtacact tgcgatgccc tgattgtcgc
     9661 aacaggcgcg tccgccaaat acctcggttt gccgagtgag gaagcgtttg caggaaaagg
     9721 cgtttccgct tgtgccacct gcgacggttt cttctacaaa aaccaagatg ttgccgtagt
     9781 tggcggcggc aatacggcag ttgaggaagc gctctacctt gccaatatcg ccaaaaccgt
     9841 taccctgatc caccgccgca gcgagttccg tgccgaaaaa atcatgatcg acaaactgat
     9901 gaaacgcgtg gaagagggca aaatcatcct caagctggaa agcaacctgc aagaagtact
     9961 gggcgacgat cggggcgtaa acggcgcatt attaaaaaac aacgacggtt ctgagcaaca
    10021 aattgccgtc agcggcattt ttatcgccat cgggcacaag ccgaataccg atattttcaa
    10081 aggtcagttg gaaatggacg aagccggtta cctgaaaacc aaaggcggca cggcggacaa
    10141 tgtcggcgca accaatatcg aaggcgtatg ggcggcgggc gacgtaaaag accataccta
    10201 ccgtcaggcg attaccagcg cggcttccgg ctgtcaggcg gctttggacg cggaacgctg
    10261 gctgggcagc caaaacattt gaatgccgtc tgaaaccgaa attttcggcg gaatgcaaat
    10321 ttttcgcacc aaatccttga ttccgtcggc aaaccccaat ataatgcctg ctctttgaac
    10381 cttgccttct gctttcgcat ggcagaaggc tgttttacca tccataagga gataacatgc
    10441 gtcattacga gatcgtgttt atcgttcatc ctgatcaaag cgagcaagtg cctgctatgg
    10501 ttgaacgtta caaaaccatg attgccgaag caaacggtaa gattcaccgt ttggaagatt
    10561 ggggtcgccg ccaactggct tacccgatta acaaaatcca taaagcccat tacgttttga
    10621 tgaacatcga aaccactccc gaagtggttg aagagctgga aaccgcattc cgcttcaatg
    10681 atgccgtatt gcgccatcta accatcaaaa ccaaacacgc cgttactgaa gcatccccta
    10741 tgttgggtgg tgaaaaggct aagaacctgt tgagcggtgc gtctgaagaa gcggtcgccc
    10801 aataattggg attcaataat cttgtttcgc ttgccgcgtt aattgaaaag gttttcccta
    10861 ttcgatatac gcctgccgga atccctgttt tagatattat tttaaagcac gaatcgtggc
    10921 aggaggaaaa cgggcagcaa tgccttgtcc aattggaaat tccggcacgg attttaggca
    10981 ggcaggcgga agagtggcag tatcggcaag gtgtatatgt tcacgtcgaa ggttttttag
    11041 ctcaaaaaag cagacgttcc cttatgccga tgctcaggat acaaaatatt caagaatata
    11101 aaggttaaac gacaatggct cgtcaatcat tcaaacgtag aaaattctgc cgtttcacgg
    11161 ctgaaaaaat ccaagaagtc gattacaaac aagttgattt gctgaaagac tttatctctg
    11221 aaaacggtaa aatcattcct gcacgcatca caggaacgaa ggcattctac caacgccaat
    11281 tggctgttgc cgtaaaacgc gcacgcttcc tggctctgct gccttacacc gaccaacaca
    11341 aataattttg gagattgaat catgcaaatt attctgttag aaaaaatcgg cggtctgggc
    11401 aacttgggcg atatcgtaac cgttaaaaac ggctacgccc gcaactttct aattcccgca
    11461 ggtaaggcaa aacgtgcgac cgaagcgaat atgaaagagt ttgaagtacg ccgcgccgaa
    11521 ttggaagcca aacaggctga aattttggca gatgcccgag tccgtcagga aaaattggac
    11581 ggtcaaaccg ttaccgttgc tcaaaaagcc ggtgtggacg gtcgcctgtt cggttccgtt
    11641 accaatgccg acattgctgc tgcaatcgtt gctgccggca tcgaagccgt gaaagcaaat
    11701 gtacgtctgc cgaacggtcc tctgaaagcc gttggcgagt acgaagtgga agtggctttg
    11761 cacactgatg ccgttgctaa aattaccgtt gccgtcgttg ccgcaaccga gtaattgaaa
    11821 ttatcagatg ccgtctgaaa ccctgccgat tgtcggaatg tggtttcaga cggtatttgt
    11881 tttgttttcc gtatattctg caaaagtcgg gatggttgtt tttatagtgg attaaattta
    11941 aaccagtaca gcgtacctgg cttcaatata ctcaatagga taggtctctt cctaaccgaa
    12001 aaaataattg tcttttccct tgcaaataat tgaaatcaac agattactaa aacacagcca
    12061 aaatattcaa ttgacaaaaa cttttctgat tttgagaaat ctgtgaaaat caggggatta
    12121 tttattttga aaatggtatc gggcataaaa tttggctgtg tttcaatatg gtgctgataa
    12181 catactttgt ttcaaaatgt tcaataggat aacttcctga ttgaccgaaa aataatggcc
    12241 ttttcccttg caagcagttg aaatcaacag attgtcaaaa tacatcctaa aaatttaata
    12301 acttatatat atgattgcaa aggttgcggc ttgtatgccg gtcattatgg cggaaatacc
    12361 gtttgggagg cagccgcatc gcgtggaatc ggcacggcag gcgatgcgcc gatgccttcg
    12421 ggcgtgaagg cggggcaatc ggcagtttgg aaaaaggcaa tggaatctgt gggcagaaga
    12481 agttaagggt aaaagtcggg cgcacggtca ggttgtctgt ccttttgtcg gaacaggaga
    12541 ctgggcagct ttgtcctgat taattgcttg tgcaaacggt aaaaaggttt tttcgtgtgt
    12601 tttaaagcag gttgtacagc atttttccgg caatcaaaag cagcataatg ccgaaggatt
    12661 ttttgagttt ggcagaagaa agtttgtggg cggttttgac accgagcggg gcaaaggcaa
    12721 tggttgccgc gctgaggacg gcgacggcgg gcaggtaaag gaagcccagt gacccttcgg
    12781 gcaatcctgc aatattcagg ccgttgagca gatacgatat tgcgccggag agtgcaatcg
    12841 gccaggcaag gccggatgat gtgccgatgg ctttatgggc ggggaagccg cagtggatta
    12901 agaaggggac ggaaagtgaa ccgccgccta tgccgaccca gctcgacatt gtgccgaaca
    12961 gtgtggaaac cgcagtcagt ccgggcagct tgggcagcgg gcgggatgcc gtctgacgac
    13021 cggtatgcag tgttttgaat gcgacggcgg ttaaaaacag gatgaagaaa atttgaagcc
    13081 cgaacgcggg gatatatttt gcggagagtg cgcccgtgaa tacgccgaat atcatacccg
    13141 gcatcatcgc aaatacggtt ttccagtcga ccgcccgttt tttgtgctgc cccaacatac
    13201 tggaaaaggc ggtgaagacc atgacggcga aggatgtgcc gacggcgagg tgttgcgcgt
    13261 aaggatgttg tgccaaaccc tgcaaatcaa gcacccataa aacgacaggg acaatcagcg
    13321 tgccgccgcc tacgccgaac agaccggcaa taaaacctgc cgcactgcct acggcaagca
    13381 ggattaagat aatgtcccaa tgccacattt aatccgcctt tctgtatttt ttccaaaatc
    13441 ggcgtgccgc cataatgtag ccggacaggc tgtatccgag aaagaacagg aagaggacga
    13501 gcgacggttc ccaagtgacc agaagcagca gtaagacggc aagcagcatt ccgacaaagg
    13561 ggacttgtct gcggatgttg atttctttaa aactccaaaa agggatttgg acaatcatcg
    13621 acaggccggc aaacagtgtg atgcccaatg cccaccagtg gacggcgggg aatttttcga
    13681 cgctgtggtt gacccaaatc agcccgacaa tcagcgcggc ggcagtcgga ctgggcacgc
    13741 cgataaacca gcgtttgtcc accttgccga tgagtgtgtt gaacagggcg aggcgcaggg
    13801 cggcgcaggc gcagtagatg aaggcgacgg aataaccgat tttgccgaac tgccaaagct
    13861 gccatttgta ggcaatcaga gcgggagcaa cgccgaagct gaccatatcg gcaaggctgt
    13921 cgagctgctc cccgaacgcg ctttggctgt tggtcagccg cgccacgcgc ccgtccatac
    13981 cgtccagcaa catagagatg aataccgcga tggcggcggt ttcataacgt ccgtgcatgg
    14041 attgggtgat tgcataaaat gcggaaaaca gcgcggcgat ggtaaaggag ttgggcagca
    14101 ggtagatact gttttgacgc agggagcgtt gcggggtgtt ggtcggggtt tccatagctg
    14161 aagtgtctca cggacgtgga gaatcgatag gggattataa cccaaaccat tatcgaacaa
    14221 tacgcccttt aggatgaagc cctcggaggc ggtttgtttt tctgatggcg gatgctttgc
    14281 ggggcggttt gactctgcct gccaccgggt gtgttgtttc ggatttttgc cggatggata
    14341 ttgttgccgg gcatttctgg gcttgccgga aggtatcggg gggagggcat cggaatatgc
    14401 ggcgcaccgt tcaaaccggg gcggcgtttc cctattttca gggggttgca ggtttcaaca
    14461 aattgaaaga tattttttgc atttccgacc gtatgctgat tttgaagcgg gataaattat
    14521 aggacaaggt ttttcgcccg ccgcaaaggt tggccgcaat gccgtctgaa aacaacggct
    14581 tgccgctaag gcggcggtat gcggatttgc gctgccgaaa tcggtttgcc atacccggcg
    14641 gtgcaatctg cggggaggga taagccgata aggcgggact tatcctgttt tttgtttata
    14701 atgcaacaga atttggacga accgtcggga atttatcatg atgaaaaaga tactggccgt
    14761 atcggcacta tgcctgatga ctgcggcggc acgggctgcc gatacttacg gctatctcgc
    14821 cgtttggcag aatccgcaga atgcggacga tgttttgcag gttaaaacca caaaagaaga
    14881 ttcgacgaaa agcgaagcgt ttgccgagtt ggaagccttc tgcaaaggtc aggacacgct
    14941 tgcgggcatt gccgaagacg agccgaccgg atgccggtcg gtcgtgtcgc tgaacaatac
    15001 ctgtgtcgcg ctggcatacc cgaaagcctt gggcgcgatg cgcgttgaaa acgccgtcgt
    15061 gattacttct ccgcgtttta cgagcgttca tcaggtcgca ctcaaccagt gcatcaaaaa
    15121 atacggcgta cagggacaat gcggcttgga aacagtgtat tgcacatctt cttcttatta
    15181 cggcggaact gtgcgctctt tgattcaaaa tctcaaataa aacggaaaat gccgtctgaa
    15241 agatgttcag acggcatttc tatatcgacg gtcaggattc tttcggatcg ggcagcaggc
    15301 tgttcaacat aatggcaagt acggcgcaca agcccacgcc ggcaaagctg aagctgccca
    15361 atttgagcgt catgccgccg atgcccgtgg tcagtaccga gctgacgatg accaggtttt
    15421 tcggcagcat caaatcgact ttggcatcaa tcaacgtttt cacgcccaaa gaagcaatcg
    15481 tgccgaacag cagcagcata atgccgccca ttactggcat cggaatggaa gccaaaaacg
    15541 cattgaattt gccgaaaaac gccatgcaga cggcaaaaac cgccgcccaa gtcatgatga
    15601 cggggttgct gtttttggta atcatcaccg cacccgttac ttcgccgtag gtcgtaaccg
    15661 gcgggccgcc gatcagaccc gcaacgcata cgcccaaacc gtcgcctgca agggttttgt
    15721 ccaagcccgg gtctttcgta tagtctttcc ccgtcacatt gccgattgcc atgatgccgc
    15781 cgatgtgttc gatggcgggg gcgacggcaa cgggcagcat aaacagtgca gcctgccagt
    15841 tgacctgagg cgtttcaaaa tggggaacgg cgaaccaggg cgcgtgtgca atgcttgccg
    15901 tgtccaccag tcccatcagc agtgccaaaa cataacccga agcgacaccg atcaagatgg
    15961 gaatcagctt catcatcctg ctgccgaaaa ccgatacgat ggcggtaacg gcaaaggtaa
    16021 agccggaaag aatcagcgaa tcggtatagt cgatgacctg tttgccgtcc gcctgaccca
    16081 ttgccatgct gcttgccgcc acggcaacag acagaccgat gaccatgatg acggggccga
    16141 tgacgaccgg aggcagcagt ttgtgtaccg ctgccagtcc gcgccaacgg atcagcgcgg
    16201 caaacacaaa atacataaag ccggcggcaa acagtccgaa catggtggaa ggcagccccc
    16261 attcgccgac ggagtagata atcggtgcga taaaggcaaa cgaagaacca agaaaaatcg
    16321 gcactttgcg tttggttgtg atttggaaca gcagcgttcc caagcctgcg cccaaaagcg
    16381 caagagccgg attcagaccg gtcagcaggg gaaccagcac cattgcgccg aatgccacaa
    16441 ataaaatctg cgcgccggaa acggcaagtt tcagttggtt cataccatct tctttcaatc
    16501 ggttcaaacg gcacggatta tactggtttg ccgagggtct gcgtaaaaat acatatggcg
    16561 ggcaacagat atgccgtctg aaaggcaatg tctgtctgtt gcaggatgaa agcaggagaa
    16621 atggagtata atccgacact ttccatattc aatccgcaag ccgtccggct tccttatttc
    16681 agacggcatt ttccggtaat acggcatgac agaaaaatcc cataaaaaaa cagcaaaagg
    16741 cagggcggga agcccgtccc caacgtccgc acgcaacaag aaagccgaca acggcgcacg
    16801 gggcaataaa gtttccgagc ggctcaaggc ggtcaaagag ttgcagaaaa ccgaaaccaa
    16861 aaaggcgcgt cccgaacatg tcgtcaacct tatcggcgac gcactgtggc tgatgggttt
    16921 ggcggcaacc ttgtatttgg cgatttccct gattagtttc gatatgggcg atccgtcttg
    16981 gtcgcacagt tcgccggttg tggaagatgt cgccaattgg ggcggactgt tcggcgcgta
    17041 tgttgccgat gtcggctatt atcttttcgg ctggtcgttc tggtggtgga tagcggctgc
    17101 ctgcgtcatg ctgtataaaa attttcgtct gcacgcaaaa cagacggaaa acgaggcata
    17161 caaccacaaa atcgctgccg ccgcgctgtt tgtcctgacg gtcttcagcc ccgtcttgga
    17221 gtattttgtg ctgggcggaa aatatgccga ctccctgcct gtcggagcag gcggtatggt
    17281 cggcatacgc gtcggcgcag tgtttgcgtg gctgctgggg aaatcgggca gcctgctgat
    17341 tatcttggtt gttctgctgt tgtcgttgtc cctgctggtg cagatttcat ggctggaatt
    17401 tttgaacggt gcgggcaggg cggttcaaaa ccgcctgagt gccttatccg gcaaggtcat
    17461 ggctttggga aaacgccggc cgaataccaa aacagacggt gtcgataccc aaaatacacg
    17521 gcgcatggta aaagaagcca agaatattac ggccaaaccc gttgccttgc ccgaaggcag
    17581 cagcagcaac cgcaaatccg tcgcggtttc cgtcgcgccg tcgcccaaaa ttcaggtttc
    17641 tctgtttgaa gatgacgaac ctcggcaggc gggcgaatac cacaagccta cattgaacct
    17701 attgcggatt cctgacagcg aacccgtcag catcaatccc gccgaattgg agcgcactgc
    17761 cgaactgatc gaatccaaac tggcagaatt cggcatcggc gtacaagtcg tatccgccac
    17821 atccggtccc gtcatcacgc gctacgaaat cgaacccgcg caaggtgtta aaggcagcca
    17881 aattgttgcc ttgtcgaaag atttggcacg ctctatgtcg ctgcaatccg tgcgtatcgt
    17941 cgaaaccatc gcaggtaaaa acacgatggg catcgagttg cccaacgaca aacgccaaga
    18001 cgtgatgttg agcgaaatct tgtcctcgcc cgtgtttgcc gaagccaaat ccaagctgac
    18061 cgtcgcgctg ggcaaagaca ttgccggtac ccccgttgtc ggcgacttgg cgaaaatgcc
    18121 gcaccttttg gtcgccggta tgactggttc gggcaagtcc gtcggcgtga acggcatgat
    18181 tatgtctatg cttttcaaag ctacgcccga cgaagtccgc tttatcatga tagacccgaa
    18241 aatgcttgag ttgagcattt acgacggcat tccgcacctg ctctgtcccg tcgtgaccga
    18301 tatgcgcgaa gcggggcagg cgttgaactg gtgcgtcgcc gaaatggaaa aacgctaccg
    18361 cctgctttcc cacgccggtg tacgcaatct ggaaggcttc aaccaaaaag tcgaagccgc
    18421 aaaagcggca ggcaagccgc tgctcaatcc gttcagtctg aaccccgaca gccccgagcc
    18481 tttggaaaaa ctgccgatga ttgtggtcgt tatcgacgaa cttgccgacc tgatgatgac
    18541 cgaacgcaaa gccgtcgagc agcaaatcgc ccgtctcgcc caaaaagcgc gcgccgccgg
    18601 tatccatatg attgtcgcca cccaacgtcc cagtgtcgat gtcgttactg gcctgattaa
    18661 agccaacatc ccgacgcgta tggcgtttac cgtgcaaagc aaaatcgaca gccgtaccat
    18721 cctcgaccaa atgggcgcgg acgaactgct caaatatggc gattcgctgt tcctccagcc
    18781 cggcagtgcc gaaccgaccc gcctgcaagg cgcgtttgtt tcagacgacg aagtacatca
    18841 agtcgtcaac tacgtcaaat cgcaagcccc agccgactat attgaaggtc tgctcagcgg
    18901 cgaggccgcg ctggaaactg ccaatatcgt taatccgaat gcagacagcg acgaattgtt
    18961 cgatcaggca gtcgcctatg ttttggaaag caaaaaaacc tccatttcgt ctttgcagcg
    19021 gcagttgcgt atcggctata accgcgcggc aaacctgatg gaggcactgg aaaatgcggg
    19081 tgtcgtttct cccaccgacc tcaacggcag ccgtaaaatt ttggcgcaca aggaccattt
    19141 gtagctcgta ttgcaaaatg ccgtctgaac ggcggaattg gcgtttcaga cggcatatta
    19201 tgtttcaggc ggaacattgt gatatacttg ccagctaaaa tctccccttt gcggcaatgc
    19261 ggttcaaata tccgtaccgt tgcgctgttt gcttccccat gcagggaaga aagtttacca
    19321 ttttatcaac acaacaaatt taagggctta tgatgagcgt aactgttgaa actttagaaa
    19381 atctggaacg caaagtagtg ttgtccctgc cttggtccga aatcaacgca gaaaccgata
    19441 aaaaactgaa acaaacccag cgccgtgcaa aaatcgacgg tttccgcccg ggtaaagcac
    19501 ctttaaaaat gattgcccaa atgtacggtg cgagcgcgca aaacgacgtg atcaacgagc
    19561 tggtgcaacg ccgcttccac gatgttgccg ttgcccaaga gttgaaagtg gcaggcttcc
    19621 cccgttttga aggcgttgaa gaacaagacg ataaagaatc tttcaaagtt gccgccattt
    19681 ttgaagtgtt ccccgaagtc gttatcggcg atttgtctgc acaagaagtt gaaaaagtaa
    19741 ccgcttccgt cggtgatgcc gaagtggacc aaaccgtaga aatcctgcgc aaacaacgca
    19801 cccgcttcaa ccatgtcgaa cgcgaagccc gaaacagcga ccgcgtcatc attgactttg
    19861 aaggcaaaat cgacggcgaa ccttttgcag gcggcgcatc caaaaactac gccttcgtat
    19921 tgggcgcagg tcaaatgctg cctgaatttg aagccggcgt agtcggcatg aaggctggcg
    19981 aaagtaaaga cgttaccgtc aacttccctg aagaatacca cggcaaagac gttgccggta
    20041 aaactgccgt gttcaccatt acgctgaaca acgtttccga agcgactctg cctgaagtcg
    20101 atgcagattt tgcaaaagcc ttgggtattg cggatggcga cgttgccaaa atgcgcgaag
    20161 aagtgcagaa aaacgtaagc cgcgaagtgg aacgccgcgt aaacgaacaa accaaagaat
    20221 ccgtaatgaa cgcgctgctc aaagccgtag agctgaaagc acctgttgct ttggtcaatg
    20281 aagaagccgc acgcttggca aacgaaatga aacaaaattt tgttaaccaa ggtatggctg
    20341 atgctgccaa cttggatctg cctttggata tattcaaaga acaagccgaa cgccgcgtat
    20401 ctttgggtct gattttggcc aaactggttg atgaaaacaa actggaaccg actgaggagc
    20461 aagtcaaagc cgttgtcgcc aacttcgcag aaagctacga agatcctcaa gaagtaatcg
    20521 actggtacta cgcagagcct tcccgcctgc aagccccgac ttctttggcg atagaaagca
    20581 acgtcgttga tttcgttttg ggtaaagcca aagtaaacga aaaagctttg tcttttgacg
    20641 aagtgatggg cgcgcaagcc tgattatcct gaaaatgccg tcttagggca gtttttgaaa
    20701 gcaccgaagc cgcttttaaa gcccgctttg gtgcttttac atcatgaaag gagacgaaat
    20761 gtcttttgat aactatcttg tccctaccgt tatcgagcag agcggtcgcg gtgagcgtgc
    20821 attcgatatc tattctcggc ttttgaaaga gcgcatcgta ttcttggtcg gcccggtaac
    20881 cgacgagtcc gccaatctgg tggttgccca actgttgttt ttggaaagtg agaatccgga
    20941 taaggatatt ttcttctata ttaactcgcc aggcggttcg gtaacggccg gtatgtcgat
    21001 ttacgacacc atgaatttta tcaagcccga tgtatcgact ttgtgcttgg ggcaggcagc
    21061 aagtatgggc gcgttcttat tgtcggcagg cgaaaaaggc aaacgtttcg ccctgcccaa
    21121 cagccggatt atgattcacc agcctttaat cagcggcggc ttgggcggtc aggcatccga
    21181 tattgaaatt cacgcacgcg aactgttgaa aatcaaagaa aaactcaacc gcctgatggc
    21241 gaaacattgc ggccgcgatt tggcagattt ggagcgcgac accgaccgtg ataatttcat
    21301 gtccgccgaa gaggctaaag aatatggtct gattgatcaa atcttggaga accgtgcttc
    21361 tttacagttt taataagaaa tctgagagaa acaaatgtcg tctgaaaaca gttcaggacg
    21421 gcatttgtct tatagggact gtatagggat gattttgttc tggttgcgtt tggagccgtt
    21481 ttcaccatag ttggttttga catattcctc tacaatagct aggaattcag ttgcaatatt
    21541 atcacctttc agtgttattt tgcgctcccc gtccacataa accggcgcga cgggtgtttc
    21601 tcccgtaccg ggaaggctga tgccgatgtc ggccaatttg ctttctccgg gcccattgac
    21661 aacgcagccc attacggcaa cgttcaggga ttcaacccca ggataaaggg tacgccatat
    21721 agacattttt tggcgcaggt aattttgaac atcttgtgcc agctcttgaa atacggtact
    21781 ggtggtacgc ccgcagccgg ggcaggcggt aaccatcggc gtaaacgaac gcaatcccat
    21841 agtttgtaaa atctcttgcc cgacgacgac ctcctgagta cgcgggctgc caggttccgg
    21901 agtcagtgaa atgcggatgg tgtcgccgat tccttcttga agcaagacgg ataatgccgc
    21961 cgttgatgcg acaatgcctt tgctgcccat accggcttcg gtcaaaccca aatgcagcgg
    22021 ataggcgcaa cggctgccca gttcgcggta aacctgaatc aaatcctgaa ccgcgctgac
    22081 tttgcacgac aggatgattt tgtcttcggg cagtcccaat agaacggctt tttcggcaga
    22141 ttccaaagcg gagacaatca gtgcttcctt cgtcacttct tcgggcggtt tcggcgcgga
    22201 agaagcgagg ttggcatcca tcatacgttt ggcgaggctt tgatccaaag aaccccagtt
    22261 tacgccgatg cggacggctt tatcgttttc agcagcagtc cgaatcataa aggcaaattt
    22321 ttcatcgcct tttacgcctt tgccgacatt gccgggattg atgcggtatt tggacaatgc
    22381 tttgccgcat tcgggaaatt ccgccaacag gcgttcgccg ttgaagtgga aatcgccgat
    22441 aagtggtgtg gcatagccca tatcgtccaa gcggcggcgg atttcggcaa ctttggacgc
    22501 ggcttcgggg ctgttgacgg taatacgcac catttcggat ccggcatcgc tcaattcctt
    22561 aatctgcaat gcggtggctt ttgcatcggc agtgtcggtg ttggtcatag attggataac
    22621 gacgggtgct tctgaaccga cggtaatatg atcgatgcgg acttgatgcg tcttgcggcg
    22681 ttggagtgtg ttcatatggt ttgaatctgt ttattgaccg atgaggacgg tttgcaattc
    22741 ttccgagtag gggaaattcg cctgcaattg tgcttcatat tcgtatgccg cctgtgcgtt
    22801 gccgagggct ttggcaattt tccagcctag cagcaaatca tcggcctgaa ggacttctac
    22861 cctgctttgg tattttttaa agtagtaatc ggcatcgccc aactgcccgg ccagcatttt
    22921 ggtgcgcgcc agttctttaa atgcgggtgg gaactgcggc tgggcggcga gggaacgttt
    22981 caaataggct tccgccaatc cgaattgccc ctgttttgcg ctgcatatgc ctttattcag
    23041 gttggcaata taaggggtcg ggtaggtggg gtcggccagg gctttgtcga aatatgccat
    23101 agattcggca gggcggttga gcctgccgca caggaaccag ccgtagttgt tgttgatttc
    23161 ggcactgtcg ggtttgatgg agagggcttg ccggaaactt tcctgcgcct tgtcgttaac
    23221 tttcaggtat tgatagattt cggcacggac cagccaggca agctcgtttt tagggtccga
    23281 tttcagggcg tcttcaatac ttgccgtcgc ctgacggtag tcctgaccgc gcatatattc
    23341 cattgccaac tgggttttga tattggaaac ctgattggct ttttctgccc gcgaggggcg
    23401 gtaggaagtg ctgcacgcgc ccaaggcaag gacaagtaat aaagagattc gtttggatgg
    23461 cttaaaaggc ataattaccc ctgttgtccg attaaaatct gctgccattt ttgttggcgg
    23521 cgcgttttat cctgaacctg ccccgccaac tgtccgcagg cggcatcgat gtcgtcgccg
    23581 cgcgtttttc gtacggtaac gacaaatcct gcctgctgca aaatatcgcg gaacacacgg
    23641 atgttctcat tgctggagcg ttcgtatcca gagtttggaa agggattgaa cggaatcaga
    23701 ttgaacttgc agggaacatc tttgaccagt tcgatcagtt cgcgcgcatg ttgcgcctta
    23761 tcgtttattc cgtccaacat gacgtattcg aaagtgatga aatccctggg tgctttgact
    23821 agatagcgtt ggcatgcagc catcaattct ttcaagggat attttttgtt caacggtacg
    23881 atttggttgc ggacttcgtc attggaagcg tggagggaaa ccgccaaagc caccggcatg
    23941 acatcgcgca acctgtccat ttggggaacc atacccgaag tggaaacggt tacgcggcgg
    24001 cggctcaaac cgtagccgtg gtcgtccagc atgatgctta aggcggtaac gacattgtcg
    24061 aagttcgcca tcggctcgcc catacccatc atcactacat tggaaattac gcgttcgttt
    24121 ttcggtgtaa cacccatcgc tttgtttgcc caccacaatt gcccgatgat ttcggcagca
    24181 gtcaaattgc ggttgaagcc ctgccggccg gtcgaacaaa atgtacattc caaagcgcag
    24241 ccgacttgtg aggaaatgca gagcgtgccg cgatccgatt cggggatgaa gacggtttcc
    24301 accccgttgc ccgtaccgac atccaaaagc cattttcgag tgccgtctga agatttttga
    24361 gacatcatca gcttgggaat ttcgatgcct gcctgttcgt tcagtttatg gcgcaacgat
    24421 tttgccaaat cggtcatttc gttaaaattt tgcgcgccgg attggtgcat ccaacgcata
    24481 acctgtttgg cacggaaggg tttttcgccc atatcggcaa aatgtcgggt cagcccttga
    24541 aggtcgtagt tgagcagatt ggttttcatg tgtttgtgtt tttcttaaat taaggcttca
    24601 aataaaaaat tgcagggcag gcaagtttga tttgaagcct tttgcgacag agttttcaga
    24661 cggcatggat atatttatgc cgtctgaaga ggagttgtgc ggtgtattgt atcaacgggg
    24721 gcagatttcg gtttggctga aaaagtaggc aatttccaaa gcggcatttt ccacgctgtc
    24781 ggaaccgtgt acggcattaa tgctgaccga agtggcaaag tccgcgcgta tcgtgccttc
    24841 ggcggcttcg gaaggattag ttgcacccat cagttcgcgg tttttcagga cggcgttttc
    24901 accctctaat acctgaatca taaccggacc gccggtcata aattcaacca atccggcgta
    24961 gaaggggcgg tctttatgaa ccgcataaaa ttcttgcgcc tctttgagag taagctgctt
    25021 cattttggcg gcaacgattt tcagaccgtt ctcctcaaag cggctgtata ttttgccgat
    25081 aacatttttg ccgacggcat cgggtttgat gatggagatg gtacgttcaa tcgccatgct
    25141 atatccttat ttttacttaa gaagaatcaa atcggtattc tatcaaaaaa ataacttatc
    25201 cgcctgaagc cttgggagat agggtcaggt gcggctgttc caaataaact tcggactgca
    25261 tttcgaccat ccggctggcg gtgcgggtaa attcttcggc aaaatcgcct tccgtataga
    25321 tatggttgac atctaccgcg ccggtggcac acagtttgac ccggaaatcg tagagtacgt
    25381 caatcagcca agtcagccgc cgcgcctccg ccttttcttg cggtgagagt tgttccaaac
    25441 ctgaaataaa aaccatttca taatgttcgg ccaaatacag atagtcggac tgtgagcggg
    25501 ggccgaagca cagtgcgcgg aaatcaaacc atatggcacg gccggactcg gctttgtggg
    25561 gaatctcccg accatggatg gtgctgatgc cggggttcaa atcggtaatg cctgtcattt
    25621 ctttgaacag ttttgccagt tttgcctcat tttcttcatt ggcaggcgta aagaaaatct
    25681 cggcggggcg gagggtacgc agtcggtagt cttcaccgcc gtcaacgttt aagacggtca
    25741 ggctggactc gatgagcgcg attgtgggaa gaaaactgct ccggttttga ccttgcgggt
    25801 agagttcgga aggcgcgtag tttgaagtcg ccaccaaaac aacgccctcg ttaagcaggt
    25861 tttccagcag acggcctaaa atcattgcat ccgcaatatc gctgacatga aattcgtcaa
    25921 aacataatac gcgggtttct ttggcaatct cggcggcaac ggatttcaac gggttgcttt
    25981 cgcttttcag ggttttcagc ctctggtgga tttctgccat aaaggcatga aagtggacgc
    26041 ggcgtttgcg gcggtaaggg aggcagccga aaaaagcgtc catcagaaag cttttgccgc
    26101 gtccgacccc gccatagaaa taaagccctt tggggacttg cggggaacgc aaactcctgc
    26161 ctaaaaaacg gtttcttttg cgtttgaaca tcatcaattc ggtccaaagc cgatcgaggt
    26221 gttcgatggc ggctgcctgc gcgtcgtcgc ggatgaagtt gggcagttgt gaggcagcct
    26281 gataccaggt cagcgggctg tggttttcaa acggcggggc tttaaaaagt tggtctctat
    26341 tactcatatt taaccttgtt atacttcttg cggcggagaa agaaaatgct taccgtcggt
    26401 attataaatg atttccttca aacttggcat tttccgtgga aaagaataat gacgcgcaca
    26461 agggggtata agcaaaggcg gcgtttcaat atggtgcaga tatgagctca aatgcctttt
    26521 atagt
//
LOCUS       DAWEWP010000008        26473 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWEWP010000008 DAWEWP010000000
VERSION     DAWEWP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26473
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.008"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1183..2631
                     /gene="gnd"
                     /locus_tag="WGN53_000224"
     CDS             1183..2631
                     /gene="gnd"
                     /locus_tag="WGN53_000224"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ0139264.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTGIIGAYSLQDLVDKLEKPRKIMMMVRAGSVVDDFIEQLLPLLEKGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGMGVSGGEEGARHGPSIMPGGDERAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2694..3965
                     /gene="waaA"
                     /locus_tag="WGN53_000225"
     CDS             2694..3965
                     /gene="waaA"
                     /locus_tag="WGN53_000225"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ0139265.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRGERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGVLMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRSLIRPAVASLTGCLVQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTAFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSYEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            4006..4455
                     /locus_tag="WGN53_000226"
     CDS             4006..4455
                     /locus_tag="WGN53_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ0139266.1"
                     /translation="MSIYALAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLPILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4488..5462
                     /locus_tag="WGN53_000227"
     CDS             4488..5462
                     /locus_tag="WGN53_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0139267.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5531..5606
                     /locus_tag="WGN53_000228"
     tRNA            5531..5606
                     /locus_tag="WGN53_000228"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5564..5566,aa:Lys,seq:ttt)
     gene            5826..7079
                     /gene="murA"
                     /locus_tag="WGN53_000229"
     CDS             5826..7079
                     /gene="murA"
                     /locus_tag="WGN53_000229"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ0139268.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            7160..8338
                     /locus_tag="WGN53_000230"
     CDS             7160..8338
                     /locus_tag="WGN53_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ0139269.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFTADAEAV
                     VKDIADVAEDDMILDIGPKSAAELADLLKAACTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(8405..8653)
                     /locus_tag="WGN53_000231"
     CDS             complement(8405..8653)
                     /locus_tag="WGN53_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0139270.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8744..9661)
                     /gene="ftsX"
                     /locus_tag="WGN53_000232"
     CDS             complement(8744..9661)
                     /gene="ftsX"
                     /locus_tag="WGN53_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ0139271.1"
                     /translation="MSIIHYASLHIESARTALKELLRQPFGTLLTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRIANVRFIGKEDG
                     LAELQSNLDQNLVSMLDGNPLPDAFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSTVRPLVDAIFKPYGLNIGWRFFYPEEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9658..10308)
                     /gene="ftsE"
                     /locus_tag="WGN53_000233"
     CDS             complement(9658..10308)
                     /gene="ftsE"
                     /locus_tag="WGN53_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ0139272.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10502..10984)
                     /locus_tag="WGN53_000234"
     CDS             complement(10502..10984)
                     /locus_tag="WGN53_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0139273.1"
                     /translation="MKYLNLAAIALAATFAAHTASADELAGWKDGTAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            11108..11461
                     /gene="arsC"
                     /locus_tag="WGN53_000235"
     CDS             11108..11461
                     /gene="arsC"
                     /locus_tag="WGN53_000235"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ0139274.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIAEHPALLERPIVLANG
                     KAAVGRPLENIEAIL"
     gene            11458..12114
                     /locus_tag="WGN53_000236"
     CDS             11458..12114
                     /locus_tag="WGN53_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ0139275.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIDSVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            12230..13624
                     /locus_tag="WGN53_000237"
     CDS             12230..13624
                     /locus_tag="WGN53_000237"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ0139276.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITTNEKLAEMVKPRLA
                     LRDIHETSKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEASAR
                     MLRFAERLEGLEDWNTEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13645..14040
                     /locus_tag="WGN53_000238"
     CDS             13645..14040
                     /locus_tag="WGN53_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139277.1"
                     /translation="MPSDVGIRLQTAFKRKLKMKKIFYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            14044..14535
                     /locus_tag="WGN53_000239"
     CDS             14044..14535
                     /locus_tag="WGN53_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0139278.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14658..17252)
                     /gene="mutS"
                     /locus_tag="WGN53_000240"
     CDS             complement(14658..17252)
                     /gene="mutS"
                     /locus_tag="WGN53_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ0139279.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17696..18700
                     /gene="gap"
                     /locus_tag="WGN53_000241"
     CDS             17696..18700
                     /gene="gap"
                     /locus_tag="WGN53_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0139280.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPSGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18770..18889)
                     /locus_tag="WGN53_000242"
                     /pseudo
     CDS             complement(18770..18889)
                     /locus_tag="WGN53_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18958..19638)
                     /gene="tsaA"
                     /locus_tag="WGN53_000243"
     CDS             complement(18958..19638)
                     /gene="tsaA"
                     /locus_tag="WGN53_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ0139281.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLNRIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEIRFQIEENRATV
                     IDLSPTPL"
     gene            19753..20388
                     /locus_tag="WGN53_000244"
                     /pseudo
     CDS             19753..20388
                     /locus_tag="WGN53_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
     gene            20426..21394
                     /gene="waaC"
                     /locus_tag="WGN53_000245"
     CDS             20426..21394
                     /gene="waaC"
                     /locus_tag="WGN53_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ0139282.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEVGRLKNLAQPYYAALHATSRDSKLWSMENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQTLMDCVAADKG"
     gene            21620..22369
                     /locus_tag="WGN53_000246"
     CDS             21620..22369
                     /locus_tag="WGN53_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ0139283.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIECGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22380..23315
                     /locus_tag="WGN53_000247"
     CDS             22380..23315
                     /locus_tag="WGN53_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ0139284.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAVDYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            23378..23989
                     /locus_tag="WGN53_000248"
     CDS             23378..23989
                     /locus_tag="WGN53_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ0139285.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFTYPIRTLADVKKYLDLLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(24041..24358)
                     /locus_tag="WGN53_000249"
     CDS             complement(24041..24358)
                     /locus_tag="WGN53_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ0139286.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLSTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24516..25787
                     /gene="purD"
                     /locus_tag="WGN53_000250"
     CDS             24516..25787
                     /gene="purD"
                     /locus_tag="WGN53_000250"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ0139287.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDIISGLDAANQIGKVFHAGTTAKEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25821..26423
                     /locus_tag="WGN53_000251"
     CDS             25821..26423
                     /locus_tag="WGN53_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ0139288.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         6364 a         6077 c         7448 g         6584 t
ORIGIN      
        1 ttggttttct gtttttgtgg gaatgacggg atgtaggttc gtaggaatga cgtggtgcag
       61 gtttccgtat ggatggattc gtcattcccg cgcaggcggg aatctagaac gtaaaatcta
      121 aagaaaccgt gctgtaaacg gcagactgat gccgtcattc ccacgaaagt gggaatccag
      181 aacgtaaaat ctaaagaaac cgttttcccg ataagtttcc gtaccgacaa ggctggattc
      241 ccgcctgcgc gggaatgacg gcggagcggt ttctgttgct cccgataaat tcctaaaact
      301 taaattttca tcattccctc aaaaacagaa aaccaaaaac agaaacctaa aatcccgtca
      361 ttcccacgaa agtgggaatc tagaacgtaa aatctcaaga aaccgttttc ccgataagtt
      421 tccgtaccga caaggctgga ttcccgcctg cgcgggaatg acggcagagc ggtttctgtt
      481 ttttccgata aatgccgcaa tctcaaatcc cgtcattccc actttcgtgg gaatgacgga
      541 atgtaggttc gtgggaatga cgcggtacag gtttccgtta cgtatcccca tccgaaagtt
      601 tatttataca caagcaatcc aaagttatct gactgaaata ttgtgagatt tacccgcaaa
      661 tctcattttt tccaccactt aatacacaaa ttcggatgcg tactctgcca atatgcagaa
      721 aaaagatatt tgtatcgaca cattctcatt cctacaagtt ccaaacacac ttccctctca
      781 aatctccaca acattcccgt tcttaaaggc atcggcagcc attcggtaaa gaaaagcgga
      841 atgccatctg cacaacaagc aaactgtcca caaaaactat tcttcatgaa aaacaatcca
      901 caggtatgca caaaagggaa tccgccttac cgacaccctt ttaaaacgga aatcccaatg
      961 atttttaaaa taaaaaaaag ttacccacag aaaatttctc ctatcaatat aatcatcgtt
     1021 tttttattta atttttatat tttatttata agccttcagg gaaacgcacg gtaaagcgta
     1081 aaggcatctt ttcaaaaaat taaaaagtta aggattgtta aacaagtgta aatcaggtag
     1141 agttgcggtc gataaattaa atctataacg gagcttcctg caatgaaagg cgatattggc
     1201 gtaatcggtt tggcggttat ggggcaaaac ctgattttga atatgaacga ttgcggattt
     1261 aaggttgtcg cctacaaccg aacaatcggt aaggtggacg aatttttaaa cggcgcggca
     1321 aaagggacgg gcattatcgg cgcgtattcc ctgcaagact tggtcgataa gctggaaaaa
     1381 ccgagaaaaa tcatgatgat ggttcgcgca ggttcggtgg ttgacgactt tatcgaacaa
     1441 ctgcttccgt tgttggaaaa aggcgacatc attatcgacg gtggcaatgc caattacccc
     1501 gacacgacac ggcggacaca ttaccttgcc gaaaaaggga ttttgtttgt cggcatgggg
     1561 gtctccggcg gagaggaagg tgcgcggcac ggcccgtcta tcatgccggg cggagatgag
     1621 cgcgcatggg aagctgttaa accgattttc caggcgattg ctgcgaaaac ccctcaaggt
     1681 gagccgtgtt gcgattgggt cggcagggat ggtgcgggac atttcgtcaa aatggtgcat
     1741 aacggcatcg aatacggcga tatgcagttg atttgcgaag cgtaccagtt catgaaagac
     1801 ggtttgggac tgtcctacga cgaaatgcac cgcgtgtttg ccgagtggaa caaaaccgag
     1861 ctggattctt atctgattga aatcacagcg gcaattttgg gctataagga cgaaggcggc
     1921 gaaccgctgg tcgagaaaat cctcgatacg gcagggcaaa aaggcacggg caaatggacg
     1981 ggcatcaatg ctttggattt gggtattccg ttgacgctga tttccgaagc ggtattcgcc
     2041 cgctgcgttt cgtcgttcaa agaacagcgc gtgcagaccg gcaagctgtt cgcccgaacc
     2101 gttacgcccg tcgaaggcgg caaacaagaa tgggttgagg cgttgagaca ggccctgctt
     2161 gcatccaaaa tcatttccta cgcacaaggc tttatgctga tccgcgaagc gggcgaaagc
     2221 tacggctggg atttggacta cggcaacact gcgctgctgt ggcgcgaggg gtgcatcatt
     2281 cgcagcgcat tcttaagcaa tatccgcgac gcgtatgaaa acaatcccga tttggtgttc
     2341 ttgggtgcgg atccgtattt caaaaatatt ttggaaaact gcctgccggc gtggcgcaag
     2401 gtggttgcta aggcggtcga atgcggcatc cctatgcctt gcatggcttc ggcgattacg
     2461 ttcttggacg gctacacgac cgaacgtctg cctgccaatc tcttgcaggc acagcgcgac
     2521 tacttcggcg cgcacactta tgaacgcacc gacaaaccgc gcggcgagtt tttccatacc
     2581 aactggacgg gcaagggcgg agataccgct tcgacaacct acgatatttg attccgttgc
     2641 cgaaatgccg tccgaaccgt tcggacggca tttgttttgc aggaaataga aagatgttcc
     2701 aatggcttta tgatgtattg tggctgcttg cgccgatatg gatacggcgt tatttggaca
     2761 aacgctccgg aagtgccccg gcatatcggg cgcatcgggg cgagcgtttc ggcaagccgt
     2821 atccgaatcc cgttaccggc gcggtttgga tacacgccgt ttcggtcggg gaaacgcgtg
     2881 ccgcccaacc tttgatacgc gagttgcggc agcgttttcc cgatgcgccg ctgctgatga
     2941 cgcagatgac cccgacggga cgggaaaccg cgcaagttct gtttcccgat gcgcaatgcc
     3001 gctatcttcc gtatgacaaa aaaacgtggg tacggcagtt tttgcgcgaa caccgcccga
     3061 tgttcggcgt attgatggaa acggaaatct ggcccaacct aatgaaggaa tgccggcgcg
     3121 cgggtgtgcc gctgtttttg gcgaatgcgc ggctgtcgga aaaatcgttg aacggttatc
     3181 tgaaagtccg cagcctgatc cgtcctgctg tcgcttcgct gacgggctgt ctggtacaga
     3241 cggaggcgga tgcggcgcgg ttggcgaaat tgggcgcggc atccgtgcag gtgtgcggca
     3301 ataccaaata cgacctgatg ccgtcggaac agatgaaaac gctggcgggg cagtttgaaa
     3361 aacgcatcgg agaccggccg gttgccgtgt gcggcagcac gcgcgtttat cggggtgaag
     3421 acgaggcgga gaaactgctg gcggcgtggc aacaatatcg cggcgatgcc ctgctggtcg
     3481 tcgtgccgcg ccatcccgag cattttcaga cggcatttga aacggcaaaa cgcttcgggt
     3541 ttaaggttca gcggcgcagc gacggtttgc cggtcgaacc cgatacgcag gtgtggatag
     3601 gcgacagtat gggcgagctg tatgcgtatt acctgtgcgc cgatgttgct tttgtcggcg
     3661 gcagtctggt cgattcgggc tgtcagaaca tcatcgaacc gctttcctgc ggcgttccga
     3721 cgatattcgg cttttcgacc tacaattttt ccgaagcctg ccggcacgcc ttggcatcgg
     3781 gtgcggcggt tcaagtcgaa tcggcggacg cgtggcggga agccgttgaa aaaaccttat
     3841 cgtacgaggg gggcggaatg cagatgcagg cgcgcgtgga cggctttatc gcacaacatc
     3901 gcggagcggg cgcgagaatc gccgaggcgg tgcgggaagc ggtatgcgga catcgggggc
     3961 gatagtgata caatccgcat ccgagttttc cggttggagt accgtatgag tatttacgca
     4021 cttgcacaca tcgtccatct gtattgcgcc attgcctttg tcggcggcgt gttttttgaa
     4081 gtgctggttt tgtccgtcct gcatacggga cgggtgtcgt gcgaggcgcg gcgcgaagtg
     4141 gaaaaggcaa tgtcttaccg cgccgtcagg gtgatgccgt ttgtggtcgg actgctgttc
     4201 gccagcggcg tcgtgatggc ggcaaaccgc taccttccta tattgggcga accgtttgcc
     4261 acttccttcg gtacgatgct gacgctgaaa atcctgttgg cgttcagcgt gttggcgcac
     4321 ttcgccatcg ccgtcgtcaa aatggcgcgt tccacactga cggtcggctg gtcgaaatac
     4381 atacacgccg tcgtctttac ccatatgctg ctgattgtct ttttggcaaa agcgatgttt
     4441 tatatcagct ggtaacacaa ctacatttca aatcaggaga ttgaaccatg acagaatatc
     4501 ccggcatcgg gtcgccgttg ttttacggcg ttttttttgc ggcagtgctg gtcatgattg
     4561 ccttggatat gttttcgctg aagaaaaacg gcagccataa agtcggcgtc aaagaagcct
     4621 tggcatggag cggcttgtgg gttgccgtat cctgcctgtt cgcgggctgg ctgtatttcg
     4681 aactcgccgg caatctcggc tacggcgcgg cagtcgcaaa agaaaaagta ttggaattct
     4741 ttaccggcta cattttggaa aaatcgttgg cagtcgacaa tattttcgtg ttcctgatga
     4801 tattcggcta cttcaaagtc gcaccacagt ttcagcaccg cgtgctgctg tacggcgtat
     4861 tgggcgcatt ggtattgcgc actgtcatga ttttcgtcgg cgcggcactg gttcggcagt
     4921 tcgagtggat tctgtatctg ttcggcgcgt tcctgctcta taccggcata cacatgatga
     4981 agcccgaagg cgatgaggaa gaggatttgg caaacagcag gctgctgaat gccgtcaaga
     5041 aagtcgttcc ggtcggcacg gaatttcacg gcgagaaatt ttttaccgtc gaaaacggca
     5101 aaaaaatcgc cacgccgctg tttttggtgc tagtcatgat tgaattgagc gatgtcgtgt
     5161 ttgccgtgga cagcatcccc gccgtctttg ccgttaccac cgatccgttt atcgtgctga
     5221 cctccaatat tttcgccatt ttgggtttgc gggcgatgta tttcctgctg gcggatgtgg
     5281 cggaacgctt tatcttcctg aaatacggct tggcattcgt gttgggtttt atcggtgtga
     5341 aaatgctggt aatgcattgg gtgcatatcc cgatttccgt ttcactgtcg gtcgtgttcg
     5401 gcgcgttggg tgcatcgata ctgacctcgt taatttatac gaaaaaacag cctgataaat
     5461 aaaatgccgt ctcaacgccg acaaaatggt tgacagcatc aggcttgatg tatagaattt
     5521 cgagcttgtc gggtcgttag ctcagtcggt agagcagcgg acttttaatc cgttggtcga
     5581 gcgttcgaat cgctcacgac ccaccagata aaacaaaaaa agctgagttt tcaaaactcg
     5641 gctttttttg ttttccgaca atccttgttc ggaaagcgcg gcatttcgtc aaattctttg
     5701 gtgcggcttc cgtatttttc ccgaaaatcc cttataattc cgcctctgca accttcatcg
     5761 ggtgatgctt tatgcgttgt cccgagaatt gaaatattac aagtacccac caggaaagaa
     5821 caaacgtgga caaactgaaa atctccgcaa acggcccact caacggggaa ataacggtct
     5881 cgggcgcgaa aaacgcggca ttgccgctga tgtgcgcggg tttgctgaca tcgggtacgt
     5941 tgcgcctgaa aaacgtccct atgctggcag atgtgaaaac cacgcaaaag ctgcttcagg
     6001 ggatgggcgc gcgcgtcctg accgacaata tcagcgaatt tgaaattaac ggcggcacgg
     6061 taaacaatac ctgcgcccct tacgaattgg tacggacgat gcgcgcctcg attttggtgc
     6121 tgggcccgac gctggcgcgt ttcggcgagg cgcaagtcag cctgccgggc ggctgcgcca
     6181 tcggttcgcg ccccgtcgat cagcatttga agggcttgga agcgatgggg gcggaaatcg
     6241 tcatcgaaca cggttacgtc aaggccaaag gcaaactcaa aggtacgcgc gtggcgatgg
     6301 atgtcgttac cgtcggcggc acggaaaacc tgctgatggc ggcgactctg gcggaaggta
     6361 cgacggtttt ggaaaactgc gccatcgagc ccgaggtggt cgatttggcg gaatgcctgg
     6421 ttaaaatggg cgcgaaaatc agcggcatcg gaacatcgac catgatcgtg gaaggtgtgg
     6481 acgagctgca cggctgcgaa cacagcgtcg tccccgaccg gatcgaggcg gggacgttcc
     6541 tgtgcgcggt ggcgataacc ggtggcaggg tggttttgcg gaatgccgcg ccgaaaacga
     6601 tggaagtggt gttggacaaa ctggttgagg caggtgcggt gattgaggcg ggcgacgatt
     6661 ggatcgccat cgatatgcgg cagcgtccga aggcggtgga catccgcacg gtcgtccacc
     6721 ccggcttccc cacggatatg caggcgcagt ttatggcatt gaatgccgtg gcggagggaa
     6781 gctgccgcgt ggtggaaacg atttttgaaa accgctttat gcacgtcccc gagttgaacc
     6841 ggatgggggc gaacattaca accgagggca atacggcatt tgtgcagggt gtggaacggc
     6901 tttccggcgc agtcgtcaag gcgacggatt tgcgtgcgtc cgcaagcctc gttatcgccg
     6961 gtttggcggc gcgcggcgaa accgtggtcg aacagattta ccacttggat cgcggttatg
     7021 aaaatattga aaaaaaactc ggcagcgtcg gcgcgaaaat cgagcgcgta tccggctgat
     7081 cgggttgcga tgccgtctga agccttcaga cggcatcaat tgtttctgta tcgcaaacac
     7141 tgattgaaaa ggactgatta tggcattttt aaaactgacc gaacaaaacg tgcagggcaa
     7201 aaccgtcctc atccgcgccg atatgaacgt gccgttcaaa gacggcaaaa tcagcgacga
     7261 cacccgtatc cgcgcctcgc tcgcgtccat ccaatactgc atggacaacg gcgcatccgt
     7321 tatcgtgatg acccacttgg gccgcccgac cgaaggcgag ttccaccccg aagacgacgt
     7381 tgcgcccgtt gccgcgcatt tgggcggcct gttgggcaaa aacgtgaaag tattgaacga
     7441 ctggcgcgaa aacaaaccgg ctttgaacgc gggcgatgtc gtgatgctgc aaaacgtgcg
     7501 tatcaacaaa ggcgagaaga aaaacgattt ggaactgggc aaagcctacg cttctttgtg
     7561 cgacgtgttc gtcaatgacg cgttcggcac cgcgcaccgc gcccaagcct cgaccgaagc
     7621 cgtcgcccaa gccgctcccg ttgcctgcgc cggcgtattg atggcgggcg aactcgacgc
     7681 tttgggcaaa gccttgaaac agcccgcgcg cccgatggtt gccatcgttg ccggcagcaa
     7741 agtgtccacc aagctgacca tcctcgaatc gctggcggac aaagtcgacc aactcatcgt
     7801 cggcggcggc attgccaaca ccttcctgtt ggcggaaggc aaagccatcg gcaaatcttt
     7861 ggcggaacac gatttggtgg aagaatccaa aaaaattatg gcaaaaatgg cggccaaagg
     7921 cggttctgtg ccgctgccga ccgatgtcgt cgttgccaaa gcctttaccg ccgatgccga
     7981 agcggtcgtg aaagacattg ccgacgttgc cgaagacgat atgattttgg acatcggccc
     8041 gaaatccgcc gccgagcttg ccgatttgct caaagctgcc tgtacggttg tatggaacgg
     8101 ccctgtcggc gtgtttgagt tcgatcaatt cgcaggcggc acgaaagctc ttgccgaagc
     8161 cattgctcaa agcaaagcat tctcgattgc aggcggtggc gacacgctgg cggcgattgc
     8221 taaattcggc gttaccgacc aaatcggcta tatttctacc ggcggaggcg cgtttcttga
     8281 gttcttggaa ggtaaagagt tgcctgccgt agccgctttg gaaaaacgcg gcgcgtaagc
     8341 ggtttgatat aaaaaatgcc gtctgaaacg ttgtgctgtt tctgacggca tttttgtgtg
     8401 gcgattattg tgctttggct gcccattccg ccggagtggc ggcaaccgaa atggaaagtg
     8461 cgtgcagttc gttactttcc agttgggctt tgagtccgtc tttaatcagg cggtggcgcg
     8521 cgaggcgtgc cttgccttca aattctgatg aaacgatgac ggcgaaaaaa tggtgtccgt
     8581 cgccttctac ttcgatatgt tcgcattttg ccacgccttc aatcatggct tttacttgtt
     8641 ccgaggtcag catggtaggt tccttgttgg aaaaatgcgc cattatactg gcaaattgaa
     8701 atgccgtctg aaaacgggtt cggacggcat ttttgacggt gttttatttt ttggctttga
     8761 agccgagcag gtgctgggtg gtggcaagcc acgcgccgaa tacgcccaac gcgatgacga
     8821 agccgaacac cagcgcgatt tcttcaggat aaaagaaacg ccagccgata ttgagcccgt
     8881 agggtttgaa gatggcatcg accaaggggc gcactgtgga aagcagccaa ccgcaaagcc
     8941 ccaggctgac ggcggcggaa aggatgctct gccacattgc ctgataaagg aaggggcggc
     9001 ggatgaacga tgcgggcgcg cccaagagtt tggtgatttc gatttcttct ttgcggctga
     9061 ggatttgcag gcggatggtg ttgtgtgcga caaggacgaa cgccataccc agcgtcaggg
     9121 aaagaaacca taaaatttta cggataaatt cgttgatttg gtacagcgtt tgcacccatt
     9181 cggtatccat agacgcggat tcgaccatag gcagtttggt aatgtcccgg tagattgcct
     9241 gcatttgcgc gggcgttgtc gcggggtcgg gggtaacgat aaatgcgtcg ggcagcgggt
     9301 tgccgtcgag catcgaaacc aaattttggt cgaggttgga ctgtaattcc gccaagccgt
     9361 cttccttgcc gatgaagcgg acgttggcga tccgtttgtc gcgcgccagc aggctgcgga
     9421 cggtatcgct gtcgctttgt gcggcggcgg tttccatata gacggtgatt tgcggcgact
     9481 cgttgagttt gcccaacacg ctttgcccgc tttgaatgcc caaatacata aacagcggca
     9541 gggtcatcgc gacggcgagc atcaggaggg tgagcagcgt gccgaagggt tggcgcagaa
     9601 gctctttgag cgcggtgcgg gcggattcga tgtgcagcga ggcgtagtgg atgatgctca
     9661 tgcgagtcgt cctttcgaga ggcgcaggat gcggtgtccg tagtccgcca tcagggtttc
     9721 gtcatgggcg gcaacgatga cggttgttcc cgcttcgtgg aaggttttga acaattccat
     9781 gatgtcgagc gcgtaggcgc ggtcgaggtt ggcggagggt tcgtcggcaa tcagcaggcc
     9841 gggctggtga acgacggcgc gggcgatgca caggcgttgt tgttcaccgc cggagagggt
     9901 tacgggatcg tccaattctc gtcctttcag gccgactttt tcgatggcga tgcgggcgcg
     9961 ttcttcggct ttgcgcggcg gatagccgat aatccgaagc ggcaggatga cgttttgcag
    10021 gacgttgcgg tcgtagagga ttttgtggtc ttggaacacg atgccgatgt gttggcgcat
    10081 aaagccgatt tggttgtcgg tcagtgtgcc gaggtcttgg ttgttgaagt ggactttgcc
    10141 cctgcttggt ttggtaatgc ccgaaatcag cttgaggacg gtggatttgc ctgaaccgga
    10201 gtgtcctgcg ataaaaatca tctcgccttt gttgatttgg aagctgacgt ttttcagggc
    10261 ttcaaaaccg ccgggatagg ttttggaaac ttgttcgaaa cggatcatag gaggtcctgt
    10321 aaggaagggc ggcatatcgt ttcgggcggt tgcgtccgca gggatatgcc gtctgaacaa
    10381 taggtaagac agtgccgatt ataaagattc cgcctgcttt tagatagtgg ataagtttgg
    10441 cggatatgaa ggtcgggcgg gaaaaatgcc gtctgaagcg gcttcagacg gcatccggcg
    10501 tttaacggca ttttgaatgg gcgagtttga cggcttcggt caggcttttt tcgttcactt
    10561 ccccggtaat ggtctgcctg tatccgcatt tcggtgcttc gacgacggta aagggcagta
    10621 cgccgacagt gtttccgtag gttttcataa agtttcggct gttcgccccg gtgtaacgcc
    10681 aaatcgggta ggaaacagga gtttgtttga ggaagttgcc gatattgtcg gatgtgtcga
    10741 gcgcgatgcc gaccatatcg acgctgcctt ttttctgcgc tttgtaccat ttggacatgg
    10801 cgggcatctc ttttcggcac gggccgcacc aagtcgccca caggttgacg atgcgtacgg
    10861 gggctttgag cgattgcagg ctttgcgcgg taccgtcttt ccatccggca agttcgtctg
    10921 ccgaggcggt atgtgcggca aatgtggcgg caagggcgat tgcggcaaga ttcaagtatt
    10981 tcatcgtttt gtcctttgta tgttgattcg ggtattgtaa accgtttggt acggttgggg
    11041 gcgggatatt gagtaaaacc ctgcgtaagc cgataataag gggattgttg agaagcaagg
    11101 aaatgtcatg cctgaaatca aaattttcca caatccgcgt tgcagcaaat cgcgtgccgc
    11161 cttgtcccta ttggaagaac gcggcattgc tgccgaagtg gtcaaatatt tggatacgcc
    11221 gcccgacttg tccgaattga aggatatttt caacaaattg ggcttggcat cggcgcgcgg
    11281 gatgatgcgt gtgaaagatg atttgtacaa ggaattgggt ttggacaacc ccgatttgga
    11341 caatgacgcg ctgctgcgtg ccatcgccga gcatcccgcc ctgttggagc gtccgattgt
    11401 tttggcgaac ggtaaggcgg ctgtcggcag gcctctggaa aatatcgaag cgatattgtg
    11461 aacaaacggc tgttttgcag ccgcaacggc ttgcgctatt acctgttggg cggtttctgt
    11521 ttgagtgttt ttccgctttt gctggtgttt gcctcatccg tttgggcggt ttatcggacg
    11581 ggcgggcagg ttttgccgcc gtatgtccgg gcggatgccg cgctggtgtt gggcgccgcc
    11641 gcgtgggaca aacgcccttc cccggttttc cgcgaacgca tcaaccacgc catcgcgctt
    11701 tatcagagcc gcagggtagg caaaatcgtg tttacgggag ggcgaaccaa aaaaggctat
    11761 atgaccgaag ccgaggtcgg gcggcggtac gcgctcaaac agggggttcc ggcgcgcaac
    11821 atcctgtttg aaaacacttc gcgcaacact tatgaaaacc tgaacaacat ccgtccggtt
    11881 ttgcgtgcca acggtattga cagcgtggtg attgtcagcg atccctacca tttggcgcgg
    11941 gcggcggaaa tggcggagga tttgggtgtc cgcgtttata tgtctgccac cccgaccacg
    12001 cggtttgatg cgggaaataa gaaaaaaatc tttatgctgc aagagggata tgcgttatcc
    12061 ctctaccgtt tggaaaaatg gggcagccgc tttttgggat ggctgtctga ttgaagcggt
    12121 agaaaatgcc gtctgaaact tatccgcagg cagggttcgg acggcatttt tgatgcccaa
    12181 gcacggtaaa accagtaaaa taaacgcttt caaagatttc ggagccatca tgaccgtcaa
    12241 aacccgtttc gcccccagcc ccaccggcta cctgcacatt ggcggcgttc gcaccgcctt
    12301 gttttcgtgg gcgtttgccc gccatcataa aggcgaattt ttattgcgca tcgaagacac
    12361 cgacttggcg cgttctaccg ccgaatccgt caacatcatc ctcgacggca tgaaatgggt
    12421 cggcctgaac tacgacaacg ccgacaatgt cgtgtaccaa acccgccgtt tcgaccgcta
    12481 caaagaagtc attgccgaac ttttagaaaa aggccatgcc tactactgct attgcagcaa
    12541 agaagagctg gaagccatgc gcgagaaagc cgaaaaagaa ggcacggcga cttacgaccg
    12601 ccgctggcgg ccggaagcgg gcaaaaccct gcccgaaatc cctgccggcg tgcaacccgt
    12661 cgtccgcttc aaaacgcctt tggatggcgt aaccaagtgg gcggacttgg tcaaaggcga
    12721 aatttccatc cccaacgaag ccctcgacga cctgattatc gcacgcgccg acggcacacc
    12781 gacctacaac ttctgcgtcg tggtggacga ctacgacatg ggcgttaccc acgttatccg
    12841 gggcgacgac catgtgaaca acaccccgaa acaaatcaac atcttaaaag ccatcggcgc
    12901 aaacctgccc gaatacggac acctgcccat gattctcaac gaacaaggca aaaaaatctc
    12961 caaacgcagc ggcgataccg tcgccattac cgatttcggc gcaatgggca tcctgcccga
    13021 agccatgctc aactatctgg cgcgcttggg ctgggcgcac ggcgacgacg agttctttac
    13081 gatggaacaa ttcatcgaat ggtttgattt gaaagacgtt tccccgtctc caagccgtat
    13141 ggacttgaaa aaactctact ggatcaacgg cgaacacatc aaaatcacca ccaacgaaaa
    13201 actggccgaa atggtgaaac cgcgcctcgc cctgcgcgat atccatgaaa caagcaagcc
    13261 tgctttggaa gacgtgttgg cattggtcaa agaccgcgcc caagatttga acgccttggc
    13321 agacgaatgc ctgtatttct acaaaaaaca agtccctgcc gaagccgatg ttgccaaaca
    13381 ctgggacgac gaagcctccg cccgtatgct gcgctttgcc gaacgcctcg agggtttgga
    13441 agactggaat accgaagcca tccacgacct gttcaaacct ttctgtgacg aagaaggtat
    13501 taaaatgggc aaactcggca tgcctttgcg cctcgctgtc tgcggcacgg cgaagacccc
    13561 aagcgtcgat gccgtgttgg cattaatcgg caaagaagaa gtgttgaaac gcatccgcgc
    13621 ttaagttatc cacaggcgtg ggtgatgccg tctgatgttg gaatacggct tcagacggca
    13681 tttaaacgga agttaaaaat gaaaaaaata ttttattttc tgatggttgt tttttctaca
    13741 agcgtatggg ccggggatgc ggaagacaat ctgctcagca tccaatccgg ttatcgtgcc
    13801 ttactgcaaa aacaaaacaa tctggacgga aaaatcatcg ggatgcagtc ggatttggaa
    13861 gatgcgcgca gacggttgca ggcggctcag gcagacatcg cccgtttgga agcggaaatt
    13921 cctgcagcaa tggcgcaaaa agcccggcag gctgaagagc tgaggcaaat cggagtgcgt
    13981 ttggaccatg cttggaatgc ggtttacggc gcagggggaa cgaaagcgtc ggggaattga
    14041 ccgatgaatt ccctctttgt ggacaatact gttttcatta cacggctgaa agccgggcat
    14101 atcggcaggt tggttcaggc gttgtttgag gagtggcacg gatttgaacc gtggtcttct
    14161 gtggataaga ttcatgccta ttacggcagg tgtttgaagg atgacgaact gccgctggca
    14221 tttgcggctg tggatgattc cggaatcctg ttgggttcgg ctacggtcaa gcggcatgat
    14281 atggaaagtt ttccacagta tgaatattgg ttgggtgatg tctttgtttt acctgaatat
    14341 cgcggaaaag gcattggcag gaggctggtc gcccattgca taggcgcagc gcgttcgctg
    14401 gggataaagt tcttgtatct ttatacgcct gatgtgcaaa tattttatga atcattcggc
    14461 tgggcggttg tcgggcgaca tttccataac ggtgaatggg ttacggttat gcgtttggat
    14521 atggataagg tttaagccga atggacaaat gccgtctgag ctgttcggac ggcattttta
    14581 tgtttggaaa cttcttgtca gataatgttt atccacagat ttttccatat ggtttgatgc
    14641 tgttccgcca acggaaatta agatacggat ttgcacaaat ctttcagacg gtacagttct
    14701 gacaatgctt cgcgcggggt caggctgtcg ggatcgagtt tttccaaggc tgctgccaat
    14761 ataccttcaa aatgtttttc ctctgctttg tccacaaagt tgcccacatt cggttcatct
    14821 cctttttcag acggcatggt actgaaaata tccagttggg gacggtttgc ggcggcttgg
    14881 ttttccagtc cgttcaaatg cttttgggcg gatttcaatg cgcgtacagg caggccggcg
    14941 agtttggcaa cggcgatgcc gtagcttttg ccggcgggac ccggttggat ttggtgcagg
    15001 aaaacgatgt cctgtccctg ttcgagcgcg gaaaggtgca tattgacggc ggcggcgtgg
    15061 gcttcgggca ggtaggtcag ctcgaaatag tgggtagcaa acaggctgaa ggatttgttt
    15121 ttttgcagca ggtgttcggc aacggcgtgc gcgagggcga ggccgtcgaa agtggaagta
    15181 ccacgtccga cttcgtccat taaaacaagg ctttgttcgg tggcgtgatg caggatgtag
    15241 gcggtttcgc tcatttcgac catgaaagtg gagcggttgg aggcgaggtc gtccgatgcg
    15301 ccgatgcggg tgaagatttg atcgatgggc ccgattgtgg cggcatcggc aggcacaaaa
    15361 cagccggtgt gtgccaataa aacaatcagc gcgacttggc gcatgtaggt ggatttgccg
    15421 cccatattgg ggccggtgag cagcatgagg cggtgtttgt ggtcaaggtc ggtgtggttg
    15481 gcggtgaagt ggcgtacctg ctgttcgaca acgggatggc ggccgttttc gatgtggata
    15541 accggatagt cggcaaactc ggggcggacg aagttccgct cttttgccaa ggctgaaaat
    15601 gtggacaaca cgtccagcgc ggcggcggct ttggcggctt tttgaagctg cggcaatgcc
    15661 gtctgaaggt ttttcaatac gccgtcaaag agttgttttt ctaaggcgag ggcttgctct
    15721 tgagcagtca gcactttgtc ttcaaaggct ttcaactccg gcgtgatgaa gcgttcggcg
    15781 tttttgaggg tttgccggcg ttggtagtcg gcaggtgctt gttcggcttg ggttttggac
    15841 aattcaatgt aaaagccgtg aacgcggttg aactcgactt tgagtgtgga caaaccggta
    15901 cgttcgcgtt ccttggcttc caaatccagc aaaaattcgt cgccatggtt ttgaatgcgg
    15961 cgcaattcgt ccagttcggg atgaaaaccg tggttgatga cattgccgtc tttcagccag
    16021 acggaaggtt cgggcaaaat ggcttggcgg agctgttcgg ctgtggatag gttttcaggg
    16081 aaaacggctt tgagggtttc caacaggctg ctgccgtttg cggacaagtc gatttgtgcc
    16141 aactcgaaca gactgtcgcg taaagatgcc aaatcgcgcg ggcgggcgtt gccgacggcg
    16201 atgcgggcag caatgcgttc aatgtccgca atgtttttca gacggcattg gaggggtttg
    16261 tattggcttt ccagcgcggc aacggcttct tggcgcgctc ggatgtgggc gcggttgcgt
    16321 aaagggtggt gcagccagag agccaagagg cggctgccca tatgggtagc gcaaaggtcg
    16381 agcgtggaca tcagggtcgg cgattttttg ccggagaggg tttgcgtgat ttcgagattg
    16441 cggcgcgtgg cggcatccat accgatatat tggctgtcgg tttcgagcga caggccgtcc
    16501 aaatgttgcg gcatcaggtt ttgcgtcaga cggatatagt tcaacagtgc acctgccgcg
    16561 ccaatcgcaa cggcgtgttc tttgccgtcc aaaccgaagc cgcgcaaatc ctggcagccg
    16621 aaatattccg tcagcagttt ttcccccgcg tcggcggcaa actgccacgc gttcaggcgc
    16681 gtaacacccg atgccgtctg aagttgcggt gcgtttttac tgtcaggcaa cagaatttcc
    16741 gccgcctgca ggcgcgccag ttcgtcgtcc aatttatcca cagttgtcag cttggttttg
    16801 aattcgccgc tttgcagcga tgcccacgcc aaaccgatgt attttttgtc gggggacacg
    16861 gcaacgatgc ggttggtttc cttgtcttcc agcaatgcgg aatcggtcag cgtgccgggc
    16921 gttacgatgc gcacgacttt gcgctccacc ggtcctttgc ctgcgccgac ttcgccgacc
    16981 tgttcgcaaa tcgccacgct tttgcccaac ttgaccaggc gcgccagata ttgttcggcg
    17041 gcgtgaaacg gcacgcctgc cattttgacc ggctcgccat ccacctgtcc gcgcgtggtc
    17101 agggtaatat ccaaaagttt tgccgcttct accgcatcgt cgaaaaacat ctcgtaaaaa
    17161 tcgcccatac ggtaaaacac cagtttgtcg gtatgttgcg ctttgatgcc gaggtattgc
    17221 tgcatcattg gggaaacggc ggatttggac atggggctgc cttatcatta ggaaaacggt
    17281 attgtagcaa aaggttttgt cttcgacgga atgagactga ccgtaagcct gtcaataagg
    17341 ttgtggagac aactaggaaa acagcaaagt tatccacaga aatattatta agtcaaaaaa
    17401 catccacgct tatctgtctt atacagcaag tcttaaactc aaacttattt gacaaaaagg
    17461 taatgaaaat attggatatt tgctacttac gcacaagcag gttggcaatc atcaaaatca
    17521 tcattaaaat tttaaattca aatattgatg gtaaaagttt tggaaatctg tgcaaacagt
    17581 aaaattcatc tatatcaaat aagcatcaag tagttagatg taatagtgga atgtaacttt
    17641 atttcagaat acaatacagg tattgaaatt tcgtttcaca gcttggagta tatacatgag
    17701 cattaaagta gcgattaacg gtttcggccg catcggccgc ctcgcattgc gtcaaattga
    17761 aaaagcccac gacatcgaag tcgtcgccgt caacgacctg acccctgccg aaatgctgct
    17821 gcaccttttc aaatacgaca gcacgcaagg ccgtttccaa ggtactgccg aattaaaaga
    17881 cgatgccatc gtggtaaacg gcaaagaaat caaagttttc gccaatccga atcccgaaga
    17941 attgccttgg ggcgagttgg gtgtggatgt cgtcctcgaa tgtaccggct tctttaccaa
    18001 taaaaccaaa gccgaagccc acatccgtgc cggcgcgcgc aaagtcgtga tttccgcacc
    18061 tagcggcaat gatgtgaaaa ccgtcgtata tggcgtaaac caagacattt tggacggcag
    18121 cgaaaccgtt atctccgctg cttcctgcac caccaactgc cttgccccga tggcggcagt
    18181 cctgcaaaag gaattcggcg tagtcgaagg cctgatgacc accatccacg cctacaccgg
    18241 cgaccaaaac acccttgacg cgccgcaccg caaaggcgat ttgcgccgcg cccgcgccgc
    18301 cgcgctcaac atcgtgccca acagcaccgg tgccgccaag gccatcggtc tggttatccc
    18361 tgaattgaac ggcaaactcg acggctccgc ccaacgcgtc cctgttgcca ccggctcgct
    18421 gaccgaattg gtttccgtcc tcgaacgccc tgtaaccaaa gaagaaatca acgccgcgat
    18481 gaaagccgcc gccagcgaat cttacggcta caacgaagat caaattgttt cttccgacgt
    18541 tgtcggcatc gaatacggtt cgctcttcga tgcgacccaa acccgcgtga tgaccgtggg
    18601 cggcaaacaa ttggtgaaaa ctgttgcctg gtacgacaac gaaatgtcct acacctgcca
    18661 actcgtccgc actttggaat acttcgcagg caaaatctaa ggcttgaaca aacctgtgga
    18721 taagtaaatg ccgtctgaac acattgcgtt cagacggcat tgtttatctg tatagtggat
    18781 taaatttaaa ccagtacggc gttaccttgc cttgccgtac tatttgtact gtctgcggct
    18841 tcgttgcctt gtcctgattt aaattcaatc cgctataact tgatattgcg gcttatggcg
    18901 aacaaacaac agccgtttgt tcaactgcta tgcggcaaaa ccggattttt gcccgattta
    18961 aagcggggtt ggggaaagat caataacggt tgcacggttt tcctcgattt gaaatctgat
    19021 ttcgtaatcc gcaatattca tcacataaat ccgttcggga atattctgat aggcggggcg
    19081 cggatcttgg gcaatgcttt ggctgataag gtttttggtg tttgcagata aattttccgc
    19141 gccgatgttt tcctgccaaa cgacttccaa ctctacgggt ttgccgctga cgaaaccaga
    19201 tgcggcatcg ggtttggatt cgacaaaggg gatataaggc ttgatgtcca caatcggcgt
    19261 gccgtcgagc aggtctgcgc cgctgcaata aaggcggacg ggtttgccgg tttcgatgcg
    19321 gttgagtttt aagagtgata gtccgagatg gttggggcgg tgggggctgc gcgtggcgaa
    19381 tacgcccatt ttttgtttgc cgccgagccg tggtgggcgc accatttgtg cccagccttc
    19441 atccaatacg ccgtgaaaaa taaaacttat ccacacataa tcgaaatctt ccagcccgcg
    19501 cacgctgtct gcggtaaatt cgggattcag ctcgatgcat acttctgcgg cggagactaa
    19561 accgggctgg cgggcgatgc cgaatttctg tttgtagggc gatcgggcgg tgccgatggg
    19621 ggtaatggta taagtcataa ggttgtggat aagttttttt ggaaaaacct tatcttatca
    19681 caatcggttt taccgttttt gggcagtaat ttgttataat acagggcatt ttaatcgcgt
    19741 tgaaataatg agatgattgt ttccattgat gttgatgcac aaaaaacgtt tacgccactg
    19801 tgtcctgacg aactgcccgt gaacgagggg catttgattg tcgaggagtt gaatgctcaa
    19861 gccgctttgg cggatttgcg cgtgatgacg aaagatgcgc atcatatggt agcgaaatgg
    19921 cttgtggata accctgttga tatgttgaag ccgacaggtt tgcctgatgc ggatttgact
    19981 tgggtggctc atgcgatggt cggtacgcgc ggctatgaat tattggacgg actgccttct
    20041 gctaaagaat atgattattg cgtttggaaa ggtgttgatc ctgaattgca tccttacggc
    20101 gcgtgttttc acgatattga ggaaaaacta agcacagggc tgattgaatg gctgcgttgt
    20161 taaaatacgg atacggttat tatcggtggg ttagctacgg attattgtgt taaaacaacg
    20221 gttttgcagt tacttaaagg tggtaattgg caggttatcg tcaatgaagc ggcttgtcgg
    20281 ggcattgcgc cggataccat tgaaacggca tggcaggaaa tgagttctgc cggtgcagtc
    20341 atcttaaaaa acgctgaaga aattaaaaaa tatattaata atcaataagt taaaattgtt
    20401 tcacgtgaaa catacttctt aaaatatgaa aattttgctt gtccgtttgt ctagtatggg
    20461 cgatttaatt cacactttgc ccgcaattga ggatttggcg cgacaatgcc ctgatgtaga
    20521 actccattgg ctgtgtgaag ctggatttgc agatattgcg cgcctgcatc cgtttgtgaa
    20581 aaaaatccat gtgatgaaat ggcggcaatg gcgcaaacat ctctttcagg ctgaaacttg
    20641 gcgggaaatg ggtcatctga aacagacttt gcggcaggaa gcatttaatt ttgtattgga
    20701 cagtcaaggt ctgattaaaa gcgcgtgttt cgctaaaatg gcgaaatccc ctatttatgg
    20761 tttggataaa aatagcgcgc gcgagggact ggctgcgctg gcgtatgcga aaacatacgc
    20821 tgtaccgaaa gggaaaaatg ctgtttggcg caatcgtgag ctgttcgccc aagtatttgg
    20881 gtatgcgatg cctgaaacgc aggtatttgg cttgactgtt cctgaggtag gccgtctgaa
    20941 aaatttagcg cagccgtatt atgcggcttt gcatgcgacc agtcgggaca gtaagttatg
    21001 gtctatggaa aactggcggt cgttgctgca aaagttgaat gaagaacagc aatgcaatat
    21061 ttacctgcct tggggaaatg aagatgaaaa aacgcgtgcc aaacagattg cagacggact
    21121 gccgtttgct attgtgtgcg acaaaataaa tttattgcag gcagcgtatc tgctgaaata
    21181 cgcggtcgga attgtcggcg tggataccgg tttgctgcat ttggcaaatg ccttggaaaa
    21241 acctgtggtc ggcatttata ccgataccga tccgattaaa acaggcgttc aggtttcgcc
    21301 aattgcaaaa aatttgggca atatcgggca gattccgacc gcagatttgg tttatcaaac
    21361 gttgatggat tgtgttgcag cagacaaagg ctaaagggtt gtctgaaact gatatagcac
    21421 ctgatatttt tcagtttgtt aagcataaga atgtatatct accagcctga gtaaatgctt
    21481 taaaaatggt ataatcggca agcattatcc gaaagatttt tgaatgaatt cgtttttaag
    21541 gaaaacggat ttggttttat agggaagaag gtttggtttc gctggatggt gaaagcagcg
    21601 ttctttttag gagggttcaa tgaaagcact ggtcgcagta aagcgcgtag tggactacaa
    21661 cgtcaaagtt cgtgtaaaag ccgatggttc ggatgtggat atcggcaatg tcaaaatgtc
    21721 gatgaatccg tttgacgaaa tcgctgtgga agaagctgtc cgtttgaaag aagccggaaa
    21781 agtaagcgaa atcgtagcgg tttctttggg tgagaaaaaa tgcgaagaaa ccttgcgtac
    21841 agctttggcg atgggtgccg accgtgccat tcatgttgaa accgatacta aactggagtc
    21901 tctggccgtt gctaagttgc tgaaagccgt tgcggacaaa gaaaatccgc aaattttctt
    21961 tttgggcaaa caagcgattg atgatgatgc caatcaagtg gcgcaaatgc tggcagcttt
    22021 gctgaatgcg gcgcaaggta cgtttgcttc aaaagtacac attgaatgtg gtgaagtaca
    22081 gattgtgcgc gaaattgatg gcggcgaaga aaccatagca ttaaaactgc ctgctgttat
    22141 cagtgctgat ttgcgtttga acgagccgcg ctttgtcaaa ctccccaata ttatggcggc
    22201 aaagaaaaaa cctttggaaa aactgactcc tgacgatttg gttgccgaca tttcacctcg
    22261 tttgaaaacg gtgaaatttg ccgaacctaa ggcgcgtcag gcaggcgtaa aagtagcaag
    22321 cgttgccgaa ttggttgaaa aattgaaaaa cgaagccaaa gtgatttgag gaggctgaaa
    22381 tgagtgtatt gattattgtc gaacacgaca acaaacagtt gaatcctacc actttgcatg
    22441 ctgttaccgc tgccgctaag ctgggtaaaa tcgatttatt ggttgccgga agcggtgcat
    22501 ctgctgtggt agagttcgca aaacaagtgg cgggtgtgga aaaagttttg gttgcagatg
    22561 ctgcccatta tgccgaaggt ttggctgaag agttggctcc gctggttgtt aaattggcag
    22621 tggattactg ctatgttgca gcaacggcaa ctacattcgg taaaaacctt ttgcctcgcg
    22681 tagcagcctt attagacgta ccgcaaattt ctgatttgac cgaaattgtg gataacacga
    22741 cttttgtgcg ccccatttat gcgggtaatg catttgaaac cgtgcaagct gattcagaaa
    22801 aattggtgct gaccttccgt gcgacggttt ttgacgcagt ggcggcgcaa ggtggtaatg
    22861 ctgaggtaat taatgttgaa gcaacccctg cccaaaacct gagtcgtttt gttaaccgtc
    22921 agctttccca ttccgatcgt cctgaactga ctcaggcaaa agtgattgtt tccggtggtc
    22981 gtgcgttggg tagtgcggaa aaattcaatg aagtgctgac accgttggcg gatgttttag
    23041 gtgcggcaat cggtgcatcc cgtgcagcag ttgatgccga atatgcgcca aacgatgctc
    23101 aagtcggaca aaccggtaaa gtggttgcgc cgcaactcta ttttgcaatc ggtatttcag
    23161 gtgcaattca acacgttgcc ggtatgcaag acagtaaggt gattgttgca atcaataaag
    23221 atgctgatgc gccgattttc aatgtagccg attacggatt ggttggcgat ttatttgaaa
    23281 tcgttccaca attaacagaa gtattgaaaa attgatgttt cacgtgaaac tttcagacga
    23341 ccttgttatt aatgaggtcg tctgaaaaca tttcagcatg attacgactt ataaaaccat
    23401 tacttcctcg acgcaggctg agtttaaaga taaaggcagc cgttttattg catttaccta
    23461 tccgattcgg acattggctg atgtgaaaaa atacctcgat ctgttaaagg aagagcatca
    23521 taaagcacga cactggtgct atgcctatcg tttgggtgtg gatggtgtgc aatttcgtgc
    23581 caacgatgat ggagagccat caggaagtgc tggacgacca attttgggac agattgattc
    23641 ggtgggtatt accgacgttt tggttgtggt cgtccgctat ttcggtggta ctttgttagg
    23701 tgttccaggt ctgatacatg cgtacaaaga ggcgacggct caagcgttgg cagttgcaga
    23761 agtagttgaa aagaatattg aaaaaactgt ttggctgaaa tgtgaatatc cgtttttgaa
    23821 tgaagcgata cgcattgcca aacaatatca ggcggatata ttggagcggg atttgcagtt
    23881 ggattgccga ttgacggtca gtttgtcgtt ggtaaattat gaggcctgtg ttgcggcttg
    23941 gaagaatact cggcagattg aagtaaatac ggaaaagcct tttgaatgaa aggtcgtctg
    24001 aaatgctttg tgatggtatt tcagacgacc tcttcgctga ctaatcttct tgtgtatatt
    24061 tgtctaaggc atcgacgctg gagcgcaaat ataaccaagc caattgatgg aattcgccca
    24121 gcgcgttcca tagcgcatct tcactcataa tgctgtattc gccttccgta ctcaaatcca
    24181 gttctacccc gtcttcaata gcctgatgac acgcctgata ctcgtgggta tagaaattgt
    24241 ccatgtcgcg aattaaagtg acgacatatt tccaacggcg gatgtcttct tccagttgcg
    24301 gtagtaaatg acaccattcg cggtatttgc tttggatttc atcaatacgt ttttgcatgg
    24361 ttgcttgtcc ttttttaatt tatggataag ttgaataggt tgcagagtgg aatgaagcgg
    24421 tcttttgttt gattctacta aaatttcttc agatgggata aaagtccctt tgtatcgagt
    24481 tttcgttaaa agtacatatt tgtcaaggag atggtatgaa actgctggtt atcggtaatg
    24541 gcggtcgcga acacgcgctg gcttggaaat tggcgcagtc gcctaaagtg gaaacagtat
    24601 ttgttgcgcc tggtaatgcc ggtacagcga ttgaacccaa gttgcaaaac atcgacttga
    24661 ctgcgcatca ggatttgatt gaattctgtc gtaaagaaaa tattgttttt accgtcgtcg
    24721 gccctgaagc gcctttggcg gcaggtattg tggatgattt ccgtgctgca ggactaaaaa
    24781 tattcggtcc gacacaatat gcggcgcagt tggaaagttc caaagatttc gccaaagcat
    24841 ttatggcgaa atacaatatt ccgaccgcgc aatatcaaac ctttgaaaac gccgatgccg
    24901 cacatgatta cgtcaatcag aaaggtgcgc ctatcgttat caaagccgat ggtttggcgg
    24961 caggtaaagg cgtgattgtg gcgatgactt tggatgaagc gcatgctgcg attgatgata
    25021 tgctgctgga caacaaaatg ggcaatgccg gcgcgcgtgt tgtaattgaa gatttcctgc
    25081 aaggcgaaga agcgagtttt atcgtcatgg ttgatggcaa taatgtactg cctatggcaa
    25141 ccagccaaga ccacaagcgt cttttagacg gcgacaaagg tctgaatacg ggcggtatgg
    25201 gtgcgtacag ccctgcgcct gtggtaacgc ctgttgtgta cgaacgcgcg atgaatgaaa
    25261 ttattttgcc gaccgtagca gggatgaaag cggaggggca tgagttcacc ggtttcctgt
    25321 acgcaggttt gatgattgat caaagcggtg caccctatac gattgagttt aactgccgtt
    25381 tcggcgatcc tgaaacccag ccgattatga gccgactgaa cagcgactta tcggatttgg
    25441 ttgaagcggc aatagacggc aaactcgata gcgtaaccgc ggaatggagc ccgcaaactg
    25501 ccgtgggcgt ggtactggcg gcgcaaaatt accccgaaac gcccaaaaaa ggcgatatta
    25561 tttccggtct ggatgctgcc aaccaaatcg gcaaagtttt ccatgcaggt acaacggcaa
    25621 aagagaaagg tgacgtattg accaacggcg gacgcgtatt gtgtgttgtg ggattgggcg
    25681 acaatgtggc gcaagcgaaa gctaaggcat atggcgcgtt ggaaaaaatc agtttcgacg
    25741 ggatgcaata ccgtaaagac attgccgata aagcgattaa ccgttaacta gaaagaggat
    25801 gatgccgtct gaagccaagt atgactgatg tgcttcagac ggtattttat attatgttat
    25861 ttccgagaat tatcgcagca tcagcgcaac gtaagttaag cgtttatttg aaaaaaggcg
    25921 gtttggtcgc ttatccgacc gaatcttgtt atggcttggg gtgtcttccg acattagcca
    25981 aagcactcgg taagttggcg catttgaaaa aaagaccgca acacaagggt atgattgtca
    26041 tcgggaatca atttgagcaa ttgcagcctt tacttcaaat gccgtctgaa aatctgcagg
    26101 atatgcttag aaaagaatgg cccgccccta aaacgttcct attatccgcc aaatcctgtg
    26161 tcttacctga gttgcgcggg aaacagcgca gcaagttggc ggtcagggtt cccgcccatg
    26221 taggtgcgcg ccgcttgtgt caggctttgc aaacgccttt ggtctcgact tcgtgcaacc
    26281 gcgcaggcaa acgcgcgtgt cggacggaga gggaagtcag aaggcaattc ggacgcgatg
    26341 tgtggattgt tggtgggaga atcggcaggc agaaatcgcc gagtcagatt attgacgggg
    26401 aaacaggtaa acgtttgcgt tagatatggg tatttgaatg aaaacttttt ttgcatacac
    26461 aaagaaaaaa ttt
//
LOCUS       DAWEWP010000009        26289 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWEWP010000009 DAWEWP010000000
VERSION     DAWEWP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.009"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            182..538
                     /locus_tag="WGN53_000252"
     CDS             182..538
                     /locus_tag="WGN53_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ0139289.1"
                     /translation="MLSAYFPVFVFILVGLAASVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            529..1011
                     /locus_tag="WGN53_000253"
     CDS             529..1011
                     /locus_tag="WGN53_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ0139290.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1024..1617
                     /locus_tag="WGN53_000254"
     CDS             1024..1617
                     /locus_tag="WGN53_000254"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ0139291.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1607..2863
                     /gene="nuoD"
                     /locus_tag="WGN53_000255"
     CDS             1607..2863
                     /gene="nuoD"
                     /locus_tag="WGN53_000255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ0139292.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2863..3336
                     /gene="nuoE"
                     /locus_tag="WGN53_000256"
     CDS             2863..3336
                     /gene="nuoE"
                     /locus_tag="WGN53_000256"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ0139293.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3726..5027
                     /gene="nuoF"
                     /locus_tag="WGN53_000257"
     CDS             3726..5027
                     /gene="nuoF"
                     /locus_tag="WGN53_000257"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ0139294.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5027..5464
                     /locus_tag="WGN53_000258"
     CDS             5027..5464
                     /locus_tag="WGN53_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139295.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINKL"
     gene            5754..8015
                     /locus_tag="WGN53_000259"
     CDS             5754..8015
                     /locus_tag="WGN53_000259"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ0139296.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAN
                     GLGVKNFATRLRQQDKRLSDDLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKKLSGNAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTADGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLQGFEYHDTAAI
                     LKDALDAESLPSKLDNRVSSTQKDFQTASSRLVRVGGVGIYHTDAIVRRSAPLQATVH
                     AAVPAARVNPNTLARLSLQDGQIAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8018..9094
                     /gene="nuoH"
                     /locus_tag="WGN53_000260"
     CDS             8018..9094
                     /gene="nuoH"
                     /locus_tag="WGN53_000260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ0139297.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGVWMISPLNLWK"
     gene            9175..9654
                     /gene="nuoI"
                     /locus_tag="WGN53_000261"
     CDS             9175..9654
                     /gene="nuoI"
                     /locus_tag="WGN53_000261"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ0139298.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            9666..10337
                     /locus_tag="WGN53_000262"
     CDS             9666..10337
                     /locus_tag="WGN53_000262"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ0139299.1"
                     /translation="MTFSAILFYILAAIVLYGALRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMEPADQVKVRADQGRMRLVKMEAVKPQTESAEESEVSDDLKPK
                     EEGKA"
     gene            10334..10639
                     /gene="nuoK"
                     /locus_tag="WGN53_000263"
     CDS             10334..10639
                     /gene="nuoK"
                     /locus_tag="WGN53_000263"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ0139300.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            10673..11248
                     /locus_tag="WGN53_000264"
     CDS             10673..11248
                     /locus_tag="WGN53_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ0139301.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            11372..11713
                     /locus_tag="WGN53_000265"
     CDS             11372..11713
                     /locus_tag="WGN53_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139302.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVSPK"
     gene            11762..13786
                     /gene="nuoL"
                     /locus_tag="WGN53_000266"
     CDS             11762..13786
                     /gene="nuoL"
                     /locus_tag="WGN53_000266"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ0139303.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKIGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQTATIQ
                     LFPSVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMMIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPVTWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQAFRPIYVLFESKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGVIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            13875..15371
                     /locus_tag="WGN53_000267"
     CDS             13875..15371
                     /locus_tag="WGN53_000267"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ0139304.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAILAVVVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            15381..16826
                     /gene="nuoN"
                     /locus_tag="WGN53_000268"
     CDS             15381..16826
                     /gene="nuoN"
                     /locus_tag="WGN53_000268"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ0139305.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRAHPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            16938..17237
                     /locus_tag="WGN53_000269"
     CDS             16938..17237
                     /locus_tag="WGN53_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ0139306.1"
                     /translation="MTASMYILLLLALIFANAPFLTTRLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(17346..18242)
                     /locus_tag="WGN53_000270"
     CDS             complement(17346..18242)
                     /locus_tag="WGN53_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ0139307.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEANALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSRGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGATACPDLSDAELSVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(18226..18450)
                     /locus_tag="WGN53_000271"
     CDS             complement(18226..18450)
                     /locus_tag="WGN53_000271"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ0139308.1"
                     /translation="MKKITPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            complement(18561..19484)
                     /gene="rsgA"
                     /locus_tag="WGN53_000272"
     CDS             complement(18561..19484)
                     /gene="rsgA"
                     /locus_tag="WGN53_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ0139309.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSMRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENADLLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELPG"
     gene            complement(19489..21345)
                     /locus_tag="WGN53_000273"
     CDS             complement(19489..21345)
                     /locus_tag="WGN53_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0139310.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPRALPLNVPQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMNGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(21371..21508)
                     /locus_tag="WGN53_000274"
     CDS             complement(21371..21508)
                     /locus_tag="WGN53_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139311.1"
                     /translation="MPSEGGATFALTKPCVSASRCLPDGFSRKMALLSFLSDGIMFDA
                     V"
     gene            complement(21508..22092)
                     /gene="ruvA"
                     /locus_tag="WGN53_000275"
     CDS             complement(21508..22092)
                     /gene="ruvA"
                     /locus_tag="WGN53_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ0139312.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVSGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHAVTDGLFAAAPAADETEDIVGTLLA
                     LGYSEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(22174..22500)
                     /locus_tag="WGN53_000276"
     CDS             complement(22174..22500)
                     /locus_tag="WGN53_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0139313.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(22554..23273)
                     /locus_tag="WGN53_000277"
     CDS             complement(22554..23273)
                     /locus_tag="WGN53_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ0139314.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVQTVLTAG"
     gene            complement(23270..23392)
                     /locus_tag="WGN53_000278"
     CDS             complement(23270..23392)
                     /locus_tag="WGN53_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0139315.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(23403..23867)
                     /gene="trmL"
                     /locus_tag="WGN53_000279"
     CDS             complement(23403..23867)
                     /gene="trmL"
                     /locus_tag="WGN53_000279"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ0139316.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(23932..24429)
                     /locus_tag="WGN53_000280"
     CDS             complement(23932..24429)
                     /locus_tag="WGN53_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0139317.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     SERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            24485..25399
                     /gene="bioH"
                     /locus_tag="WGN53_000281"
     CDS             24485..25399
                     /gene="bioH"
                     /locus_tag="WGN53_000281"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ0139318.1"
                     /translation="MCLKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQRERKSMPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TAAEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            25401..26189
                     /locus_tag="WGN53_000282"
     CDS             25401..26189
                     /locus_tag="WGN53_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0139319.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWACALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         5687 a         6699 c         7395 g         6508 t
ORIGIN      
        1 cgaattacgg tgtaaaaatt tatatgacct tataaaatca aataagaatc gttatcataa
       61 catgattgta tttattgggt ttttttgggc gttttgccga tatttacctt ttaatggttt
      121 ttgaaattcg ctaaaatacg aaattattgt agaaattttg ttaacggatt tgggtgtaac
      181 catgttgtcc gcttactttc ccgtctttgt ctttatcctc gtcggcctcg cggccagcgt
      241 gctgtttatc ctgctcggca cgattttagg cccgaaacgc cactacgccg aaaaagacgc
      301 gccttacgaa tgcggttttg aagcctttga aaacgccagg atgaagttcg acgtgcgcta
      361 ttacctcgtc gccatcctct ttatcttgtt tgatttggag gtcgcgttca tgttgccgtg
      421 ggcagtcgtg ttcaaagatt tgggcgcgta cggcttctgg tctatgctgg tgtttatcgt
      481 cgttctgacg gtaggctttg tttacgaatg gaaaaaaggt gcgctggaat gggaatagaa
      541 ggcgttttga aaaaaggttt catcaccacc agcgcggata cggtgttgaa ctatatgcgt
      601 accggttcgt tgtggccggt tactttcggc ttggcctgct gcgccgtgga aatgatgcac
      661 gcgggtatgg cgcgttatga ccttgaccgt ttcggcatca ttttccgtcc gtccccacgt
      721 caggccgacc tgatgattgt ggcgggtacg ctgaccaaca aaatggcacc cgccctgcgc
      781 cgcgtgtatg accagctcgc cgagccgcgc tgggtattgt ctatgggctc gtgtgccaac
      841 ggcggcggct attatcacta ttcttattcc gttgtgcgcg gtgccgaccg cgtcgtgccg
      901 gtagacgttt atgtgccggg ttgtccgccg actgcggaag ccctgattta cggcctgatt
      961 cagctccaac aaaaaatcaa gcgcacttcc accatcgcgc gtgacgagta agaggaggaa
     1021 gatatggcaa gcattcaaaa cttatacgaa accgtcagcc gcgttttggg caatcaggca
     1081 ggcaaagtca tttcagcttt gggcgagatt accgtcgagt gtctgcccga acactatatt
     1141 tcagtcatga ccgcattgcg cgatcatgaa gagctgcatt tcgagcttct ggtcgatttg
     1201 tgcggtgtcg attacagcac ttacaaaaac gaagcatggc agggcaaacg ctttgccgtc
     1261 gtcagtcagc tgctttccgt taaaaacaat caacgcatcc gcgtacgcgt ctgggtttca
     1321 gacgacgact tccccgtagt tgaatccgta gtcgatattt acaacagcgc ggattggtac
     1381 gaacgcgaag ccttcgattt gtacggcatc atgttcaaca accacccgga cttgcgccgc
     1441 atcctgaccg attacggctt tgtcggacat ccgttccgca aagacttccc gatttccggc
     1501 tatgtggaaa tgcgttacga cgaagagcaa aaacgcgtga tttaccaacc cgttaccatc
     1561 gagccgcgcg agatcacgcc gcgtatcgtc cgtgaggaga actacggtgg ccaataaatt
     1621 aagaaactac accatcaact tcggcccgca acaccctgcg gcgcacggcg tattgcgtat
     1681 gattttggag ttggagggcg aaaccatcgt ccgtgccgat ccgcacatcg gcctcctgca
     1741 ccgaggtact gaaaaactgg cggaaaccaa aacctatctg caagccctgc cctatatgga
     1801 tcgcttggac tatgtttcca tgatggtcaa cgagcaggcg tattgcttgg cagtagaaaa
     1861 acttgccggt atcgatgtac ctatccgcgc ccaatatatc cgcgtgatgt ttgccgaagt
     1921 aacgcgcatc ctcaatcact tgatgggcat cggttcgcac gccttcgaca tcggcgcgat
     1981 gaccgccatc ctttacgcct tccgcgaccg cgaagagctg atggacttgt acgaagccgt
     2041 gtccggcgca cgtatgcacg cagcctactt ccgtcccggc ggcgtttacc gcgacctgcc
     2101 cgacttcatg cccaaatacg agagcagcaa attccgcaat gccaaagtat tgaagcagct
     2161 caacgaatcc cgtgaaggca ctatgctcga ctttatcgac gccttctgcg agcgtttccc
     2221 gaaaaacatc gacacactcg aaaccctctt gaccgacaac cgtatttgga aacagcgtac
     2281 cgtcggcatc ggcgtcgtct cccccgaacg tgccatgcaa aaaggcttta ccggcgtgat
     2341 gttgcgcggt tcgggcgtgg aatgggacgt gcgtaagaca cagccttacg aagtgtacga
     2401 caaaatggat ttcgacatcc ctgtcggcgt gaacggcgac tgctacgacc gctacctctg
     2461 ccgtatggaa gaaatgcgtc aatccgtacg catcatcaaa caatgttccg agtggttgcg
     2521 tgtcaatccg ggtccggtca ttaccacaaa ccacaaattc gctccgccca aacgtaccga
     2581 aatgaaaaca ggtatggaag acctgattca ccatttcaaa ctctttaccg agggtatgca
     2641 cgttcccgag ggcgagacct acaccgctgt cgaacatccg aaaggcgagt tcggcgttta
     2701 catcatttca gacggcgcaa acaaacccta ccgcctgaaa atccgcgcac ccggcttcgc
     2761 ccatctgcaa ggcatggacg aaatggcaaa aggccacatg ctcgccgacg tcgttgccat
     2821 catcggtacg caggacatcg tattcgggga ggttgaccga taatgttatc cgcagagtcc
     2881 ttaaaacaaa tcgacacaga actggcgaaa tatcccgccg accaacgccg ttccgccatc
     2941 atgggtgcgt tgcgtatcgc ccaaaccgaa aaaggctggc tcgcccccga aaccatcgct
     3001 tttgttgccg actatatcgg catcacgcct gcacaagcct acgaagtcgc cactttctac
     3061 aatatgtacg accttgagcc tgtcggcaaa tacaaactga ccgtttgtac caacctgccc
     3121 tgcgccctgc gcggcggtat ggctaccggc gaatacctca aacaaaaact cggtatcggc
     3181 tacggcgaaa ctacccctga cggcaagttt acccttgtcg aaggcgaatg catgggcgca
     3241 tgcggtgatg cccccgttat gctggtcaac aaccacagca tgtgcagctt tatgaccgaa
     3301 gaagcgattg agaagaaact ggcggagttg gagtaggtcg tctgaaacga cgatttaaac
     3361 gtaggtcgga tacttgtatc cgacagagtg ggtaaaaagg caaaatgtcg gatttaagaa
     3421 tccgccctac tgaaataccg aaatgccgtc attcccgcgc aggcgggaat ccaccggtaa
     3481 gattcggttt ctgaatttaa taagacattg cttaccattg aggatggatt ctcgcctgcg
     3541 cgggaatgac gacagacaag caagtggtcg agatccaaca aaaacgatta aaggtcgtct
     3601 gaaaatatcg atttgataaa ctagatttta tttcagacga cgttacaagc cggtacacaa
     3661 agacatctta aggtcgtctg aaacagcggc cgcaaccgat acgaaaacaa acaggcacac
     3721 caaaaatggc tatttaccaa tcaggcgtga tttttgacca agtggatacc gccaatcccg
     3781 attgctggac attggacgaa tacgtcaaac gtggcggtta taccgccctg cgtaaaatcc
     3841 tgtccgaaaa catctcgcaa accgatgtga ttgacgaagt caaaacctcc ggcttgcgcg
     3901 gtcgtggcgg tgcgggcttc ccgaccggtt tgaaatggag ctttatgccc cgttctttcc
     3961 ccggcgaaaa gtacgtcgtt tgcaacaccg acgaaggcga gccgggtaca tttaaagacc
     4021 gcgacattat tatgttcaac ccccatgccc tgattgaagg catgattatc gccggttacg
     4081 cgatgggcgc gaaagccggt tacaactata tccacggcga aatttttgaa ggctaccaac
     4141 gctttgaggc cgctttggag caggcgcgtg ccgcaggctt tttgggtaaa aatattttgg
     4201 gttcggattt tgaattcgaa ctcttcgccc accacggcta cggtgcatac atctgcggcg
     4261 aagaaaccgc attgcttgaa tcgctggaag gcaaaaaagg ccagccgcgc tttaaaccgc
     4321 cattccctgc ttcgttcggc ctgtacggca aaccgactac catcaacaat accgaaactt
     4381 tctcctccgt ttcattcatt atccgtgacg gcggacaggc atttgccgat aaaggtattc
     4441 cgaatgcagg cggtaccaaa ttattctgta tttccggcca tgtcgagcgt ccgggcaact
     4501 atgaagtgcc attgggcaca ccgtttgccg aagtcttgaa aatggcaggc ggtatgcgcg
     4561 gcggcaaaaa actcaaagcc gtcattcccg gcggttcgtc cgcgcccgta ttgcctgccg
     4621 acatcatgat gcagacaaat atggactacg actcgatttc caaagcaggc tcgatgctgg
     4681 gttctggcgc gattatcgtc atggacgaag acgtgtgcat ggtgaaagcc cttgagcgcc
     4741 tgagctactt ctactacgac gagtcttgcg gccaatgtac gccttgccga gaaggcaccg
     4801 gctggcttta ccgcatcgtc caccgcatcg tagaaggcaa aggtaaaatg gaagatttgg
     4861 atttgctgga ttctgtcggc aaccaaatgg caggccgcac catctgcgcc cttgccgatg
     4921 ccgccgtctt ccccgtccga agctttacca agcatttccg tgatgagttt gtgcattaca
     4981 tcgaacacgg cggaccgatg aaagagcata agtggggagg gtggtaatgg tggaagctaa
     5041 aatttttatt ctatacggtg cagccaacaa aggtaagagt acgacactca atacgctttt
     5101 taatcagatt tgtcggaaat tttctaaatt tctagtcttt tttgaaagac atggaaacgg
     5161 cttagatttt gttgcagtat ttgatcatga aggtcagagg attggttttt attcatctgg
     5221 tgataatgaa tacgaggtta ggagaaattt atacaaactt tattcgcata attgtgattt
     5281 tatttttggc acgtcaagga cacggggtgg tagttgcgat gcagtaggat gttatgcaga
     5341 gttattgcat ggcgatgtaa atataattaa ttggtgtgaa aagtttgagc ctacagatga
     5401 agacaatgag cgtgctgtta aagagttatt taagtcattt aaaaatataa taaataagtt
     5461 atagttttag ttggttttat attggttaaa agcaaaatgc taaaaattta actttgccgt
     5521 cattcccgcg taggcgggaa tccatagtgg aatttacaga acccgatatt tgaaaagcag
     5581 ttaccgaaaa cccaagaatg gattcccgcc tacgcgggaa tgacggcaag gtggcagtaa
     5641 acgttttaaa cagtattgat tgtcaatgaa actcaaaagg ccgtctgaaa cccatttttc
     5701 agacgatctc cataaaaaat tatttatcaa atacccgtaa ctaggaacga accatgttac
     5761 aaatcgaaat cgacggcaaa caagtatctg tggagcaggg cgcgacggtg attgaagccg
     5821 cgcacaagct cggtacttat attccgcatt tctgttacca caaaaaactt tccatcgccg
     5881 ccaactgccg tatgtgtctg gtgaacgtag aaaaagcccc aaaacccctg cctgcctgtg
     5941 ccacgccggt tacagacggc atgattgtgc gtacgcattc ggcaaaagcc cgagaggcgc
     6001 aggaaggcgt gatggagttc ctgctcatca accatccgct tgattgtccg acctgcgacc
     6061 aaggcgggga atgccagttg caggatttgg cggtgggcta cggaaaaacc accagccgct
     6121 acaccgaaga aaaacgttcc gtcgtcggca aagacatggg gcctttggtt tccgccgagg
     6181 aaatgagccg ctgtatccac tgcacccgct gcgttcgttt cactgaagaa atcgccggtt
     6241 tgcaagaaat tgcgatggtg aatcgcggcg aacactccga aatcatgccc tttatcggca
     6301 aaacggtgga aaccgagctg tcgggcaacg tcatcgattt gtgtcctgtc ggcgcattga
     6361 ccagcaaacc gttccgcttc aacgcgcgta cttgggaatt gaaccgccgc aaatccgttt
     6421 ccgcccatga cgctttgggc agcaacctga ttatacaaac caaagaccat accgtccgcc
     6481 gcgtgttgcc gctggaaaac gaagcgatta acgaatgttg gctgtctgac cgcgaccgtt
     6541 tcgcctacga aggcctgtat cacgaaagcc gtctgaaaaa cccgaaaatc aaacagggcg
     6601 gcgagtggat ggacgtggat tggaaaaccg cgttggaata tgtccgcagc gcgattgaat
     6661 gtatcgccaa agacggcaac caaaaccaag tcggcatttg ggccaatccg atgaatacgg
     6721 ttgaagagct gtatctggcc aaaaagcttg ccaatggttt gggtgttaaa aactttgcca
     6781 cccgtttgcg ccaacaagac aaacgtcttt cagacgacct taaaggtgca caatggttgg
     6841 gacaaagcat tgaatctttg gctgacaacg atgccgtatt ggtagtcggc gcgaacttgc
     6901 gcaaagaaca gccgctcctg accgcccgcc tgcgccgcgc cgccaaagac cgtatggcct
     6961 tgagcgtatt ggccggcagt aaagaagaat tgtttatgcc gcttctgtct caagaagccg
     7021 cacatcccga cgaatgggca ggccgtctga aaaaactgtc tggcaatgcg gaacacgcag
     7081 ttaccgccag cctgaaaaac gctgaaaaag cagcggtgat tttgggtgcg gaagtgcaaa
     7141 accatcctga ttacgccgcg atttatgccg ccgctcaaga gctggcagat gcgaccggcg
     7201 cagtgctggg tattttgccg caagccgcca acagcgttgg tgcggatgtc ttgggcgtga
     7261 actcgggcga gagcgttgcc gaaatggcaa acgcgccgaa acaggcggtc ttgctactca
     7321 acgtcgaacc ggaaatcgat acggcggacg gtgcgaaagc cgtggccgcg ttgaaacagg
     7381 cgaaaagcgt gatggcgttt acaccgtttg tcagcgaaac gctgctggat gtgtgcgacg
     7441 tgttgttgcc gattgcgccg tttaccgaaa catcgggcag cttcatcaat atggaaggcc
     7501 gtctgcaatc ctttcacggc gtggtacaag gcttcggcga ctcgcgtccg ctgtggaaag
     7561 tgttgcgcgt attgggcaac ctgtttgacc tgcaaggttt tgaataccac gataccgccg
     7621 cgattctgaa agacgcgctg gacgcggaaa gcctgccgtc caaactggac aaccgcgtgt
     7681 cttcgactca aaaagatttt cagacggcat caagccgcct cgtgcgcgtg ggcggagtgg
     7741 gcatctatca caccgatgcc atcgtgcgcc gttccgcacc gttgcaggca accgtccatg
     7801 ccgccgtgcc tgccgcgcgt gtgaatccaa acacattggc acgcttgagc ctgcaagacg
     7861 gacaaattgc tgtcgccaaa caaaacggcg caagcgtatc ggttgctgtc aaagccgatg
     7921 ccggtttgcc tgaaaacgtg gtgcatctgc cgctgcatac cgaaaatgcc gcgctgggtg
     7981 cgttgatgga cactattgaa ctggcgggag cttgatcatg caagaatggt tccaaaacct
     8041 ctttgccgca acgctcggtc tgggcgattt gggtatcacc gtgggcttgg tggtatccgt
     8101 catcgtcaaa atcgtgatta tcctgattcc gctgattctg accgtcgcct acctgactta
     8161 tttcgaacgt aaagtcatcg gcttcatgca gcttcgcgtc ggcccgaacg taaccggccc
     8221 gtggggcctg atccagccgt ttgccgatgt gttcaaactc ttgtttaaag aggtaacccg
     8281 tccgaagctg tcaaacaaag ccctgttcta catcggcccg attatgtcgc ttgctccgtc
     8341 tttcgcggca tgggcagtga ttccgttcaa cgaagaatgg gtgctgacca acatcaatat
     8401 cggtcttttg tacattctga tgattacctc gctgtcggtt tacggcgtaa tcatcgcggg
     8461 ctgggcttcc aactccaaat attcgttctt gggtgcaatg cgtgcttccg cacaaagcat
     8521 ttcctacgag attgccatga gtgccgcgct ggtgtgtgtc gtgatggtgt cgggcagcat
     8581 gaacttctcc gacatcgttg ccgcgcaggc aaaaggtatc gcgggcggtt ccgtattctc
     8641 gtggaactgg ctgccgctgt tcccaatctt catcgtctat ctgatttccg ccgttgccga
     8701 aaccaaccgc gcaccgtttg acgtggcaga gggcgagtcc gaaatcgttg ccggacacca
     8761 cgttgaatat tccggcttcg cattcgcgct gttcttcctt gccgaataca ttttcatgat
     8821 tctgattgcc gcgctgacat cgctgatgtt tctcggcggc tggttgtctc cgttcccgca
     8881 aagctggggc tttatcggta cgccttccgc attctggatg tttgtgaaaa tggcggcagt
     8941 gttgtactgg tacctgtgga tccgtgcaac cttcccgcgc taccgttacg accaaatcat
     9001 gcgtttgggc tggaaagtgc tgattccgat cggcttcgcc tacatcgtga ttttgggcgt
     9061 gtggatgatt tcaccgctga atttgtggaa ataagtttca gacggcatct tgaggccgtc
     9121 tgaacaaagc gattttgaat acctaacgaa atccctgttt tgagggaaca taatatggct
     9181 aacttagtaa aaacctttct gctcggcgag ttggtaaaag gcatgggcgt aacgctcaaa
     9241 aacttttttg cccgcaaaga cacaatttat ttccccgaag agaaaacgcc gcaatccgtg
     9301 cgtttccgtg gcctgcacgc gcagcggcgg tatccgaacg gcgaagagcg ctgtatcgcg
     9361 tgtaaattgt gcgaggcagt ttgtccggca atggcgatta acattgaatc ggaagaacgc
     9421 gaagacggca cccgccgcac caagcgttac gatatcgacc tgaccaagtg catcttctgc
     9481 ggtttctgcg aagaggcctg cccgaccgat gcgattgtgg aaacgcatat ttttgaatac
     9541 cacggcgaga aaaaaggcga cttgcacatg accaagccga tcctcttggc aatcggcgac
     9601 aaatacgaag ctgaaatcgc caaacgcaaa gccgctgatg cgccgtatcg ttaaggagca
     9661 gacgaatgac tttttccgcg attctgttct atatccttgc cgccatcgtc ctatacggcg
     9721 cgctgcgtac cgttacagcg aaaaaccctg tccacgccgc tttgcatctg gtgttgacct
     9781 tctgcgtgag cgcgatgctt tggatgctga tgcaggctga gtttttgggc gtgacgctgg
     9841 tggtggttta cgtcggcgcc gtgatggtgt tgttcctgtt cgtcgtgatg atgttgaaca
     9901 tcgacattga agaaatgcgt gccggtttct ggcggcacgc gcctgttgcc ggtgtggtcg
     9961 gcacattgtt ggcagttgcc ctgattctga ttctggtcaa tccgaaaacc gaccttgccg
    10021 catttgggct gatgaaagac attcccgccg attacaacaa tatccgcgat ttgggcagcc
    10081 gtatttatac cgattatctg ctgccgtttg aattggcggc ggtattgctg ctgttgggta
    10141 tggtggccgc gattgcgctg gttcaccgta aaacggttaa tccgaaacgt atggaacctg
    10201 ccgaccaagt taaagtacgc gccgaccaag gccgtatgcg cttggtgaaa atggaagccg
    10261 tgaaaccgca aaccgaatct gccgaagaaa gcgaagtttc agacgacctc aagccgaaag
    10321 aggagggcaa agcatgatta ccttgacgca ttatctggta ttgggtgcgc tcctgttcgg
    10381 tatcagcgcg atgggtatct tcatgaaccg caaaaacgtg ctggtattgt tgatgtccat
    10441 tgagctgatg cttttggcgg tgaacttcaa ctttatcgcc ttttcgcaac atttgggcga
    10501 tactgccgga caaattttcg tatttttcgt actgaccgtt gctgctgccg aatctgccat
    10561 cggtttggca attatggtgc tggtgtaccg caaccgacaa acgattaacg ttgccgattt
    10621 ggacgagttg aaagggtaaa ggtaggttgg gtcgagacct gacaagacac cgatgccgtc
    10681 tgaaaacccg ataggaaaaa cgatgaaatc catagacgaa caaagcctgc ataatgcccg
    10741 ccgcctgttt gaaagcggcg acatcgaccg tatcgaagtc ggtaccaccg cgggcctgca
    10801 acagattcac cgttacctgt tcggcggctt atatgatttt gcgggtcaaa tcagggaaga
    10861 caacatttcc aaaggcggtt ttcgttttgc caacgccatg tatttaaaag aggctttggt
    10921 taaaatcgag cagatgcccg agcggacttt tgaagaaatc atcgccaaat atgttgaaat
    10981 gaacattgcc catccgtttt tggagggtaa tggcagaagt acccgcatct ggctggattt
    11041 ggtgctgaaa aaaaacctga aaaaagtcgt gaactggcaa aatgtaagta aaaccctgta
    11101 tttgcaggca atggaacgca gccccgtcaa cgatttagaa ctgcgctttc tgttaaagga
    11161 caacctgact gacgatgtgg acaaccgtga aatcatcttt aaaggtatcg agcagtcgta
    11221 ttattacgaa gggtatgaaa aaggctgagg gtcgtctgaa aagcgatttc agactgtttc
    11281 agacgacctg attcggtagg tgatcagacg ggagcggatg agaaaagaaa ttctgggtaa
    11341 gaataatccg gtctgaaata ttggaagaag aatgatggat aaaaatcagt tagaacaaga
    11401 atttcataaa gccatgttaa atatttatca ggaggctttg aatttgccgc aaccttacaa
    11461 ggcgacacga tttttacaaa ttgtaaatga atttggtggt aaagaggcgg cggataaatt
    11521 attaagtacg ggggaaaaga agactcagac cggttttaca gagctgattt tgagtggtgg
    11581 cggagtccac gccttgaaat acagtatgga atatctggtg ttacaaaagc cgtggtgtga
    11641 tttatttact gaagagcaat tagctgtggc acgcaaacga ttggagcgtg ttggatttgt
    11701 ttctccgaag taattttgta cgaaacaaac atagattttt aaatcaatcg gattcaatca
    11761 aatgaacgac atgactttat atctcatcat tgcccttgtg cctttggcag gctcgctgat
    11821 cgcgggtttg ttcggcaaca aaatcggacg tgccggtgcg catacggtta cgatactcgg
    11881 cgtggcggtg tccgccgtgc tgtcggctta tgtgttgtgg ggctttattg acggcagccg
    11941 cgccaagttt gacgaaaacg tctatacctg gctgacaatg ggcggcttgg atttctccgt
    12001 cggctttttg gtcgatacga tgacggcgat gatgatggtc gtggtcaccg gcgtatcgct
    12061 gatggtacat atctatacca tcggttatat gcacgatgaa aaaatcggct accaacgctt
    12121 cttcagctat atttctttgt ttactttcag catgttgatg ctgattatga gcaacaactt
    12181 catccagctc ttcttcggtt gggaagcggt gggcttggtg tcgtatctct tgatcggttt
    12241 ctatttcaaa cgtccgagcg cgacatttgc caacctgaaa gcctttttga tcaaccgtgt
    12301 cggcgacttc ggctttttgc tcggtatcgg cttggtgctt gcctatttcg gcggcagcct
    12361 gcgctatcaa gatgtattcg cttatctgcc taatgttcag acggccacca ttcaattgtt
    12421 ccccagtgtg gaatggtctt tgattactgt aacctgtttg ctcctgtttg tcggtgcgat
    12481 gggtaaatcg gcacaattcc cgctgcacgt ctggctgcct gattcgatgg aaggcccgac
    12541 cccgatttct gcattgattc acgccgcaac catggttacc gccggtctgt ttatggtatc
    12601 gcgtatgtcg ccgatttatg agatgagtag cactgcgctg tctgtcatca tgatgattgg
    12661 tgcgattacc gccctgttta tgggtttctt gggcgtgatt caaaacgata tcaagcgcgt
    12721 ggttgcgtat tccaccctgt cgcaactggg ctatatgacc gtggctttgg gcgcgtccgc
    12781 ctattccgtg gcgatgttcc acgtcatgac ccacgccttc tttaaagcct tgctgttctt
    12841 ggcggcaggt agcgcgatta tcggtatgca ccacgaccaa gatatgcgcc atatgggcaa
    12901 cctgaaaaaa tatatgccgg ttacttggct gactatgctg atcggcaatt tgtcgctgat
    12961 cggtacgccg ttcttctccg gcttctattc taaagattcg attatcgaag cagcgaaata
    13021 cagcaccctg ccgggcagcg gctttgctta ttttgccgtc cttgccagcg tgttcgttac
    13081 cgcgttttac gcgttccgcc aatactttat ggtgttccac ggcgaagaga aatggcgcag
    13141 cctgcctgaa catcatgacg atcatcatgg tgaagaacat cacggcttgg gcaaaaacga
    13201 caatccgcac gaaagcccgc tggttgttac cctgccgctg attttgcttg ccatcccgtc
    13261 cgtcatcatc ggctatgttg ccatcgagcc tatgctttac ggcgatttct ttaaagacgt
    13321 gattttcgtc aataccgacg cgcatccgac catgcacatc atgaaggaag agttccacgg
    13381 cgcattggca atggtgtcgc acagcctgca ttcgcctgta ctctaccttg ctattgcagg
    13441 tgtcttaagc gcatggcttt tgtacgtcaa actgccgcac ctgcctgcga aaattgcaca
    13501 ggcgttccgt ccgatttacg ttttgtttga aagcaaatac tacctcgacg ccctgtattt
    13561 caacgttttc gccaaaggca cacgcgcatt gggtaccttc ttctggaaag tcggcgatac
    13621 cgccattatc gacaacggca tcgtcaacgg ctctgccaaa ctggtcggcg taattgccgc
    13681 gcaagtgcgt aaagcccaaa ccggctttat ctacacctac gccgccgcta tggtattcgg
    13741 cgtattggta ctgctcggca tgaccttctg gggattgttc agataaggat gaggtttcag
    13801 acggtctctt aaaacgtcgt ctgacaaacc aacccgtaca acgatatttt tattttaacc
    13861 acaggttaac cactatgttt tccaactacc tactcagctt ggcaatatgg atacccatcg
    13921 ccgcaggtgt gctggttttg tcgaccggta aggacagccg tgcgccgctg gcgcgtgtgc
    13981 tcgccttcat gggtgcgctt gccggtttct tggtaacgct gcccctgttt accggtttcg
    14041 accgtttgag cggcggctat caatttaccg agttccacga gtggattccg ctgctgaaaa
    14101 tcaactatgc attgggcgtg gacggtattt cagtgctctt tatcatcttg aatgcgttta
    14161 ttacgctgtt ggtggtgctg gcgggttggg aagttattca gaaacgcccg gcgcagtata
    14221 tggcggcatt cctgatgatg tcgggtttga ttaacggtgc gtttgccgcg caggatgcga
    14281 ttctgtttta tgtgttcttc gagggtatgc tgattccgct gtacctgatt atcggtgtat
    14341 ggggtggccc gcgccgcgtt tatgcgtcgg tcaagctgtt cctctatacg ctgatgggtt
    14401 cgcttttgat gctggttgcc atggtttacc tgtactatca gacaggcagc ttctctattg
    14461 tcgatttcca aaacatcaag cagattccat tgggcgtaca acagcttttg tttgtggcat
    14521 tcttcctgtc atttgccgta aaagtgccga tgttccctgt gcacacttgg ttgccggatg
    14581 cccacgttga agcgccgacc ggcggttcga tggtgttggc ggcaattacg ctgaaactgg
    14641 gtgcgtatgg tttcttgcgt tttatcctgc cgattatgcc ggatgcggca cgctattttg
    14701 cccctgtgat catcgtattg agcctgattg ccgtgattta tatcggtatg gtggctttgg
    14761 tgcaaaccga tatgaaaaaa ctggtggcgt attcgtctat cagccatatg ggttttgtaa
    14821 cgcttgggat gtttttgttt gttgacgggc agttggacga ctgggcgttg aaaggcgcaa
    14881 tcattcaaat gatttcgcac ggtttcgtgt cggcggcgat gtttatgtgt atcggcgtga
    14941 tgtacgaccg cctgcatacc cgcaatattg ctgactatgg cggcgtggtc aatgtgatgc
    15001 ctaagtttgc ggcgtttatg atgttgttcg gtatggccaa tgccggtctg cctgcaactt
    15061 ccggcttcgt gggcgagttt atggtgatta tgggcgcggt taaagtgaat ttctgggtgg
    15121 gcgcgttggc ggccatgacc ctgatttacg gtgcgtctta taccctgtgg atgtacaagc
    15181 gcgtgatttt cggcgcgatc cacaatccgc acgttgccga aatgaaagac atcaactgcc
    15241 gcgaatttgc gattctggcg attttggcgg ttgtggtatt gggtatgggt ctgtatccga
    15301 acgcatttat cgaagtggtg catcaggcgg caaacgattt gattgcccat gtggcacaaa
    15361 gcaagatttg aggtgtgtaa atgaactggt ctgatttgaa tttgatgccc gccatgcccg
    15421 aaatcgtgct gcttgcgctg ctggtgttat tgttgctggc ggacttgtgg gtcagtgatg
    15481 acaaacgccg gtggacgcat tacggcgcgt tggcaacggt ggcggttacg gctgtggtgc
    15541 agcttgccgt ttgggaacag ggcagcacgt cttcgttcaa cggcatgtat attgcagacg
    15601 gtatgtcgcg tttggcaaaa atggttttat atgccttgac gtttgccctg tttgtttatg
    15661 ccaagcccta caaccaagtg cgcggtattt tcaaaggcga gttttacacc ctgtcgctgt
    15721 ttgccctctt gggcatgagc gtgatggtga gcgcggggca ttttttaact gcctatatcg
    15781 gtttggaact cttgtcgctc gccctttacg ccctgattgc cctgcgccgc gattccggct
    15841 ttgccgccga agccgccttg aaatattttg ttctgggcgc gctggcatcc ggcctgctgc
    15901 tctacggtat ttctatggtt tacggcgcaa ccggttcgct ggaatttgcc ggcgtgctcg
    15961 cctcttcctt caatgaagaa gcaaacgaat ggctgttgaa actgggtctg gtgtttatcg
    16021 tcgtcgccgt tgcgttcaaa ctcggcgcgg tgccgttcca tatgtgggtg cccgacgtgt
    16081 atcacggcgc gcccacttct gttaccgccc tagtcggcac tgccccgaaa gtcgccgccg
    16141 tcgttttcgc tttccgcatc ctcgttaccg ggttgggaac cgtgcatcat gactggtctc
    16201 tgatgtttgc cctgcttgcc gccgcctcgc tgctggtcgg caaccttgcc gccatcatgc
    16261 agaccaatat caaacgtatg ctcgcctatt ccaccgtatc gcatatgggc tttatcttgt
    16321 tggcgtttat ggcaggcgcg gtcggctttg cggcgggtct ctattacgcc attacctacg
    16381 cgctgatggc ggcggcaggg ttcggcgtgt tgatggtgtt gtcggatggg gacaacgagt
    16441 gcgaaaacat cagcgatttg gcagggctga accaacaccg cgtatggctt gcctttttga
    16501 tgctgctggt tatgttctcc atggcaggca ttccgccgct gatgggtttt tacgccaaat
    16561 tcggcgtgat tatggcactc ttgaaacaag gctatgtttg gttgtccgta tttgccgtcg
    16621 tgatgtcgct gattggtgcg ttctactacc tgcgcgtggt caaagtcatg tattttgacg
    16681 aatccggccg cgcccatccc accgtcggcg gcaacaatgc cgcaaaattc cttttgagcg
    16741 tcaacgcgct gctgttggtg ctgtggggca ttatgccgca aaccgttatc gactggtgcg
    16801 ccaaggcgtt ggaaaacacg ctgtaagccg ccgcaacggc agccgtgtca gaggctgccg
    16861 tttttgttaa gatatgccgt tccgcaatgc agttcagacg gcatcgccgc cgacaatgcc
    16921 caaacagaaa gcccgccatg accgcatcca tgtacatcct tttgctgctt gccttgattt
    16981 ttgccaacgc ccccttcctc acgaccaggc tgttcggcat cgtaccgctc aagcgcaaac
    17041 atttcgggca ccacttgatc gagctggcgg caggtttcgc gctgaccgcc gttcttgcct
    17101 acatcctcga atcccgtgcg ggagcggtac acgatcaggg ttgggagttt tacgccaccg
    17161 tcgtctgcct gtacctgatt tttgcgtttc cctgtttcgt gtggcggtat ttttggcaca
    17221 cgcgcaacag ggaataggca ggcataggaa tgccgtctga aacctgttca gacggcattt
    17281 gtttcattca agtgcaggcc ggcatcgctg tgccggcacg tttcagccgg cgatatacgc
    17341 cggttttaat atttgcgggc gactgcaaat tctgccaact gccgcaggcg cagggctttg
    17401 tcgccgaagg gttcgagcag cgcgaccgct tcggcaacca gtttgtgtgc gtatgagcgc
    17461 gccgcttcca agcccatcag tttcacataa gtcggcttgt cgttgtctgc gtctttgccc
    17521 gccgttttgc ccaaagtcgc cgtgtccgct tcacaatcca acacatcgtc aatgacttgg
    17581 aacgccagcc ccagttttgc cgcgtaagcg tccaatacgg aaagttccgc atctgacaga
    17641 tcaggacacg ccgtcgcccc caataaaacc gccgcacgga ttagcgcacc cgttttcagg
    17701 ctgtgcatct gttccaaatc ggcttgaacc atttgtttgc cgacattcgc caaatcgatt
    17761 gcctgaccgc ccgccatacc cctgctgccg cccgctttcg ccaacaccga caacattgcc
    17821 aactggcgtg cggcgggcag ttctgtcgga cgacttaaca cgtcgaaggc ttgggtttgc
    17881 aaagcatcgc cggtcagcag tgcgtttgct tcgccatatt tgatgtggca ggtcggtttg
    17941 ccccggcgca ggctgtcgtt gtccatcgcc ggcatatcgt cgtgaaccaa agaatagacg
    18001 tggatcattt cgattgccgc cattgcctgt tctactgctt catgcacggc ttcgcctaat
    18061 tccgaagctg ccagaaccag catcggacgc agacgcttgc cgccgtccaa agccgcatag
    18121 cgcatcgctt cgtgcagcgt gtgcgggatt tcgttttcag acggcaaaaa gcgttccagc
    18181 agcagctctg tctgtgcctg cgccctctgt tgccacgctt tcaaatcatt cgtcggattc
    18241 aaggttcaac tccttcggcc cgtccgcatc caaaacctgt aatttctgtt cgacttgtgc
    18301 cagcttggtt tggcagtacc tgaccagctc gttgccttcc tgataggcgg caagcgcgtc
    18361 ttccaagggc atttcaccct gcatagactg cgtcagcgat tcaaggcgcg acaaggcttc
    18421 ttcaaacgat ttcggggtaa tttttttcat ggtgtcggga gtttctgtaa aaacggaatt
    18481 gtagcatttc tgaaaaccgg atgccgtctg aaacagcagg atttcagacg gcattgtttt
    18541 gtcctgcaaa cggcaaggcg ttacccgggc agttcgtcgg tgatgccctg caaaaaggcg
    18601 aggcgttcgg ggcttgccgc cccggtttcg gcggcggctt tgaaggcgca gccgggttcg
    18661 gcgcggtggg tgcagttgtg gaagcggcat tgcccgacaa ggtggcggaa atcggggaaa
    18721 tagcgcggca aatcggcggc ttggaggtgg tgtaaaccaa attcttgcaa acccggggag
    18781 tcgatgagtt gggtttcgcc gttcaaatca taaagccggg cgtgggtggt ggtgtgtttt
    18841 cccgagtcga gtgcggcgga aatgtcgccg gtgcgggcgg tttggctgcc taaaagggcg
    18901 ttggtcaggg tggatttgcc cataccgctc tgcccgagca ggatgttgct gtgcccttgc
    18961 aggacggggc gcaggaggtc ggcgttttcc agcgcacggg tttcgatgac gggataaccc
    19021 agcgtttcgt agaatttgag tttttcgcgc caaagggcgg tttcgggcag gtcggctttg
    19081 ttcaggacga tgacggcttc aataccggcg gcttcggcgg caagcagggc gcgttgcagc
    19141 agccgcatgc tcggactcgg gacggcggcg gtaacgataa ggagttgggt aacgttggcg
    19201 gcgatgagtt tggttttcca cgcgtcttgg cggtagagca ggctttggcg cggtaaaaaa
    19261 tcttcaatca caacttgttc ggcgttgacg gggctgatgc ggacacggtc gccgcaggcg
    19321 aaatcgacgc gttttttgcg cgtgctggct tcgtaggttg tgccgtcggg cgtgcgcacg
    19381 atgtagcggc ggccgtagct ggcggtaatt tgggcggtgt cgttcatggt ttctttgggg
    19441 ttgggtgtgg aaatgccgtc tgaaaacggg tgttcggacg gcatcggttc agtcgtgctg
    19501 ccactcgacg tgttcgttga ggaagccgcc gctctggtgc gcccagagtt tggcgtaaag
    19561 cccgcgtttt tcgaggagtt cggcgtgtgt gccttcttcg atgatgcggc ctttgtcgag
    19621 gacgacgagc ctgtccattg cggcgatggt ggaaaggcgg tgggcgatgg cgatgacggt
    19681 tttgccgttc atcattttgt cgaggctttc ttggatggcg gcttcgactt cggaatcgag
    19741 cgcgctggtg gcttcgtcca aaagaagaat cggtgcgtct ttgagcatca cgcgggcgat
    19801 ggcgatacgc tggcgttgac cgccggagag tttcacgccg cgttcgccga cgtgcgcgtc
    19861 atagccgcgc cgccctttgg catcggaaag ttcggggatg aagccggcgg cttcggcgcg
    19921 ttcggcggcg gaaaccattt cggcatcggt cgcgtcgggg cggccgtaaa taatgttgtc
    19981 gcgcacggaa cggtgcagca gcgaggtatc ttgcgtgacc aaaccgattt gggcgcgtaa
    20041 agattcttgg gtaacgccgc ttatgtcctg cccgtcgatc gaaaccgtgc cgctttgcgg
    20101 ttcgtagaag cgcaaaagca ggttgacgat ggtggatttg cccgcgccgc tgcgtccgat
    20161 caagccgact ttttcgcccg ggcggatggt gaggttgaag ccgttgagca gcggtttgcc
    20221 ggcttcgtag cagaaatcga tgtgttcaaa tttgattgcg ccttgcggca cgttcagcgg
    20281 cagtgcccgg ggcttgtcga ggatggtgtg cggtttggac agggttgcca tgccgtcgcc
    20341 gacggtgccg atgttttcaa acagccgcgc ggattcccac ataatgtatt gcgacaaacc
    20401 gttgacgcgc aacgccatgg cggtggctgt agcaaccgcg cccacgccga cctgcccgtt
    20461 gtgccagagc cagatgccca gtgcggcggt ggagagggtc agggaggtgt tgacgatgaa
    20521 gctgcacgaa tgcagcagcg tcgccagccg catttgggcg cgcaccgtaa ccataaattc
    20581 ttccatcgac tgcttggcat aggcggcttc acgcgcgccg tgggagaaga gtttgacggt
    20641 ggcgatattg gaataggcat cggtaatgcg gccggtcatc agcgagcggg catccgcctg
    20701 ccatgcggcg gtttgcccca atttgggaat cagcaggcgc atcaccgaag cgaaaccgac
    20761 aatccagccg ataaagggca gcagcagcca tgagtcgagc gaggcgagaa tcacgccgga
    20821 ggtaatgaaa tacaccgaca cataaacgac catatcggca accgtcatca ccgcgtcgcg
    20881 caacgccagc gcggtctgca tgactttggc ggacacgcgt ccggcaaatt cgtcctgata
    20941 aaaaccgagg ctttggttca gcatcaggcg gtggaagttc cagcgcaggc gcatggggaa
    21001 cacgccctga agggtttgca ggcgcacgtt ggacgcggca aacgcccaca cgaccgaaaa
    21061 caccatcatc gccgccattg ccgccagttc ccaacctttt tcggcaaaca attcggcggg
    21121 cgcgtatttg ccgagccact ccacgatttt gcccataaat tgaaaaatca gggcttccat
    21181 aatgccgatg ccggcggtca gcgcagccag ggcggctatc catttccgca cgccggccat
    21241 gctgctccag acaaaccgcc acaagccttt ttctggcgtt ttcggggcgg cttcgggata
    21301 agggtcgatt cgggactcga accaggaaaa gattttgttc aacattgttt tcgatttcgg
    21361 taaaacagtt tcagacggca tcaaacataa tgccgtctga aaggaaggac aataacgcca
    21421 ttttacggga aaagccgtcg ggaagacagc gcgaggcgga aacgcagggt ttcgtcaggg
    21481 caaacgtcgc gccgccttca gacggcatta tttcagcagg tttttcaaag caaggcgcac
    21541 gccttcgccc acgtccgtcc ctttcggaac gcctttgacc gccgcttttg cctcgcgttc
    21601 gctgtaaccg agggcgagga gcgtgccgac gatgtcttcc gtttcgtcgg cggcgggtgc
    21661 ggcggcaaac agcccgtccg ttaccgcatg cgcgaccagc ttgccgcgca gttccaaaac
    21721 catgcgttcg gcggtttttt tgccgattcc cggggcggag gagaggcgtt tgacatcttc
    21781 ttctgcaacc gcctgcgcca gctcgtctgc cgtcatcgcc gacaaaatgc ccaaagccgt
    21841 tttcgcgccg atgccgctga ccttaatcag ttggcggaat gttttgcgtt cttccgcagt
    21901 ggcaaaacca aataaaagat gtgcgtcttc ccgaatgata agctgggtaa acagttgtac
    21961 gctttcaccc acgggcggca ggttgtagaa ggtctgcatc gatacgtcgg cctcatagcc
    22021 gacaccgttg acatcgatga cgatttgcgg agggtttttt tcaaccagtt tgccggtcag
    22081 tctgctgatc atgtatgccg aatcctgaag tgtcgggtgc aaaatgccgt ctgaaaccgg
    22141 ttcgggcttc agacggcacg gattgtatca aattcagtcg tcgcggcggg aggaaatcac
    22201 gcggccggta cgggcatcga caacgacttt gtattcctgt ccgtttttga cgatttcgac
    22261 atcatagtgc ggacggccgt tgtcgtgttc gagatcgatg tcggtgattt tgccgccgac
    22321 acgcgccaac gctgcttttt cggcttgggc gcggctgatg attttgtctt gtttgctgtg
    22381 ttggtgtgcg gcgtgtccgt ggtcgtcatc gccgtgtccg tcgtggtggg cgagtgcggg
    22441 ggcggaaatg cccatcagtg cggttgcggc ggcggtcaag agaaggcgtt tgaagttcat
    22501 gttttgctcc tgcgaagtat ctggtggtgt ttgaaagacg taaaggcggg acatcaaccg
    22561 gcggtcaata ccgtctgaac cataccgcgc gcctgagctt cggcctcggc ggcgcgttcc
    22621 tgcgaggcaa acggacccat tttgacgacg tattcgtaac ggcgtttttc aatggcgagg
    22681 ttcgggcttg atgtcgaagc ggcgaagttt tgggcggctt ggttcagata ggcttgtgct
    22741 tcgtgttccg taccgaaaga tttcaagtcg ataaagatgt ctttgttttc ggcaaccggt
    22801 gcggatttgc ccgggacgat ttgttcgatt ttgacgtgcg ccgtcccttg gctgacaaag
    22861 cccaattttt gcgcggcggc tttggatacg tcgatgatgc ggttgccgtg gaaggggccg
    22921 cggtcgttga cgcggacgat gacgcttttg ccgtttttgg tattggttac gcgcacatag
    22981 ctggggatgg gcagggtttt gtgggcggcg gtaaaggcgt tcatatcgta tcgttctccg
    23041 ccggaagttt tgcgcccgtg aaacctgccg ccgtaccacg aggcgttgcc ggtttgcgtg
    23101 aattcggcga cttggttttt cggcgtgtag cgttttccgg cgactttgta gctgcggttg
    23161 gcggaggcgt gcagtttttc tgccttgacc actgcgtcgg cggatgccgt ctgaagggag
    23221 tgtgtgccga atgcggcggt gagaaggaaa agggtttttc gggttaaagt caaaacgtgt
    23281 tccgttcttg agttgaagac gaatgggcat catgcccgcc ggatacgttc cgaaccgccg
    23341 tacagtgcgg acggcggttc ggaatgtgtc cggataggtt ttcagacggc atgaacttgc
    23401 gttcaaacgc cgcctgcgta accgtgttgc cgccacgctt caaagagaat cacggcaacg
    23461 gtattggaaa ggttcatact ccggctgccg ggctgcatgg gcaggcggat tttttgcgcg
    23521 gcgggcaggc tgtcgaggat gtcggcaggc agtccgcgcg tttccggccc gaacagtaaa
    23581 acgtcgcctt tttgaaacgc ggtttcatcg gggcgcgccg tgcctttggt ggtcagggcg
    23641 aaaatgcgcc tgcctgcgag tgccttgagg cagtcgtcga agttttcgta caccgtcagg
    23701 ctggcgaatt cgtggtagtc gagcccggcg cgtttcattt tggcggaatc caatgggaag
    23761 ccgagcggtt tgacaaggtg caaatccgcg ccggtattgg cgcacaggcg gatgatgttg
    23821 cccgtgttcg gcgggatttc cggctggtat aaaacgatgg taaacataaa tatcaatcac
    23881 ttataggcgc gtaaccttgc cacaaggcgg atggggtgtc aaaaaattta gttatttttt
    23941 cattggcgtg cgtgccagcg tccagcagca gatgcggttc gcgcccgatt ttttcagcgt
    24001 ctttgccaat tcgtccagcg tcgcgccggt ggtaaagaca tcgtcgatta acagaatatt
    24061 acagttttcc ggtatcggtg tgcggatttc aaaggcgttt ttgatgtttc gccgccgttc
    24121 gctgcctttg agcgtgcttt gcggcgggcg gtggtgtcgg aatacggtgt gtcggggcag
    24181 tatctgccag ccgtagcgtt gtgccaacaa cccgacgatg ctttcacttt ggttgaaccc
    24241 gcgttgcagc agccgctccc tgcttagcgg tacgggcagg acgaaatcga aacattcgtc
    24301 tgcaagccgg tcgggcggat tctgcatcat caggtctgcc agcggctgca ccatgctcaa
    24361 atcagccaag tgcttcagcg cgtgtatcat attgctgacg ggcggttcgt aatgcagcga
    24421 agcccacatc cggtcgaatg cgggcggttt tttctgacag ccgccgcaca ccgcgccgcc
    24481 ttggatgtgc ctgaaacaca gggggcagct gtttgccgcg tcggtgcggt atgccgccaa
    24541 atcgtcgcgg cagccggcgc agatgccgtc tgaaacgccg gacgaaccgt ggcataatac
    24601 gcaacgcctg atagtgggcg cgtctgcgat gcgccgccaa cgagagagaa aatccatgcc
    24661 tgatgccgtc aaaaaagttt acctgataca cggttggggg gcgaaccgcc acatgttcga
    24721 cgatttgatg ccgcgcctgc ctgcaacgtg gccggtgtcc gccgtcgatt tgcccggaca
    24781 cggcgacgcg cccttcgcgc aacccttcga tattgaggca gccgccgacg ctgttgccgc
    24841 acaaatcgat acgcccgccg acattctcgg ctggtcgctc ggcggattgg tcgcgctgta
    24901 tctggcggcg cgccatcccg acaaagtccg ttcgctctgc ctgacggcga gtttcgcacg
    24961 gctgacggct gccgaagatt atcccgaagg gcttgccgcg cctgcactgg gcaaaatggt
    25021 cggcgcgttc cgttcggatt atgccaaaca tatcaaacag tttctacaac tccagcttct
    25081 gcacacgcct gatgcggacg gaatcatagg cagaatcctg cccgatttgg cacggtgcgg
    25141 cacgccctcc gccttacagg aagctttgga cgcggcggaa agggcggatg cgcggcattt
    25201 gttggacaag atagatgttc cggtactgct ggtgttcggc ggcaaagacg cgattacgcc
    25261 gccgcgtatg ggcgagtatc tgcaccggca tttgaaaggc agcaggctgg ttgtgatgga
    25321 aaaggcggca cacgcgccgt ttttgagcca tgcggaagcg tttgccgcgc tgtaccgcga
    25381 ctttgttgaa ggggtttgac atgaatccgc aggacaaatg ctggcaggtt caccgccatc
    25441 tcgccgaata tgccgaccaa cgcctgacac tcgtccgcaa cgcgcccaag tatatcctgc
    25501 ttgccggtgc ggatgcggac atcagccgca gcctgctggc gaaacgctat ccgcaggcgg
    25561 tatttgaaga atacgattcc cgtgcggact ttttggcggc tgccgctgcc gcccgcaaag
    25621 gcggtttttg gcaaaggttt acgggtaagg gcgtggtgca acactgccaa tccccgatcg
    25681 cgccgctgcc cgaagcgtgt gctgatatgt tgtggtcgaa tctcggactg ttggcggcgg
    25741 aacaaatcct tcctgtgctg cacaactggg cgtgcgcctt gaagacggac gggctgctgt
    25801 tttttacctg cttcgggcgc gacactttta ccgaactgaa atgccgtctg aaagaaaacg
    25861 gcattgaaag ccgcagcgtg atgtttcccg atatgcacga cttgggcgat atgcttgctg
    25921 aaaacggctt ttacgacccc gttaccgata cggcgaagct ggtgttggat tacaaaaagg
    25981 cggaaacgtt ttgggcggat atggacacgc tgggcgtttg gcgggcgatg gcgtggaacg
    26041 atgaaaacgc cgcgcgttcg tgtgtcggga caatatttga gcgggaaggc ggtttgggca
    26101 ttacgctgga aacggtgtac ggacacgccg tgaaaaaact gatgctgccg caaggggaga
    26161 acgtggtgca gttttttccg aagagatgat gtgcagatgc cgtctgaagc cgtttccagg
    26221 tttcagacgg catttgtctg tgaaaaccga cagaaataaa ggaaatgccg atgtatagtg
    26281 aattaaatt
//
LOCUS       DAWEWP010000010        25474 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWEWP010000010 DAWEWP010000000
VERSION     DAWEWP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.010"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..617
                     /locus_tag="WGN53_000283"
     CDS             <1..617
                     /locus_tag="WGN53_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abi-like family protein"
                     /protein_id="HEZ0139320.1"
                     /translation="LSRELFHVVSIFEIVLRNKIDICLQQAFKDRNWLYNSIQPQTNP
                     ALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFDHNQLVAGLGFGFWRYLFAGGKD
                     AQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELSNINKFRNRLAHHEPICFKGAIK
                     DTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIAVCDEIDKL"
     gene            660..1436
                     /gene="lpxA"
                     /locus_tag="WGN53_000284"
     CDS             660..1436
                     /gene="lpxA"
                     /locus_tag="WGN53_000284"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ0139321.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRHAETQAELAVFRDFFAQSARGIIR"
     gene            1794..3185
                     /locus_tag="WGN53_000285"
     CDS             1794..3185
                     /locus_tag="WGN53_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0139322.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPVMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFVPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMALA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTDITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            3264..4520
                     /locus_tag="WGN53_000286"
     CDS             3264..4520
                     /locus_tag="WGN53_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ0139323.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETETGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQALFWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            4701..5510
                     /gene="zupT"
                     /locus_tag="WGN53_000287"
     CDS             4701..5510
                     /gene="zupT"
                     /locus_tag="WGN53_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ0139324.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            5607..8444
                     /locus_tag="WGN53_000288"
     CDS             5607..8444
                     /locus_tag="WGN53_000288"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ0139325.1"
                     /translation="MLDKYSPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLEKLLVKRTTGLRKPETAPKVEEASRKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVDRL
                     NQTIEQVTQAYETYRFDLAAETLYSFMWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESTDDLADYLKYLPMMTRLIEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            8586..8777
                     /locus_tag="WGN53_000289"
     CDS             8586..8777
                     /locus_tag="WGN53_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139326.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(8774..9694)
                     /gene="oxyR"
                     /locus_tag="WGN53_000290"
     CDS             complement(8774..9694)
                     /gene="oxyR"
                     /locus_tag="WGN53_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ0139327.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(9698..9961)
                     /gene="minE"
                     /locus_tag="WGN53_000291"
     CDS             complement(9698..9961)
                     /gene="minE"
                     /locus_tag="WGN53_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ0139328.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            complement(9965..10780)
                     /gene="minD"
                     /locus_tag="WGN53_000292"
     CDS             complement(9965..10780)
                     /gene="minD"
                     /locus_tag="WGN53_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ0139329.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            complement(10808..11521)
                     /gene="minC"
                     /locus_tag="WGN53_000293"
     CDS             complement(10808..11521)
                     /gene="minC"
                     /locus_tag="WGN53_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ0139330.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            complement(11668..12036)
                     /gene="rplQ"
                     /locus_tag="WGN53_000294"
     CDS             complement(11668..12036)
                     /gene="rplQ"
                     /locus_tag="WGN53_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ0139331.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            complement(12060..13046)
                     /gene="rpoA"
                     /locus_tag="WGN53_000295"
     CDS             complement(12060..13046)
                     /gene="rpoA"
                     /locus_tag="WGN53_000295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ0139332.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            complement(13072..13692)
                     /gene="rpsD"
                     /locus_tag="WGN53_000296"
     CDS             complement(13072..13692)
                     /gene="rpsD"
                     /locus_tag="WGN53_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ0139333.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            complement(13712..14107)
                     /gene="rpsK"
                     /locus_tag="WGN53_000297"
     CDS             complement(13712..14107)
                     /gene="rpsK"
                     /locus_tag="WGN53_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ0139334.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            complement(14127..14489)
                     /gene="rpsM"
                     /locus_tag="WGN53_000298"
     CDS             complement(14127..14489)
                     /gene="rpsM"
                     /locus_tag="WGN53_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ0139335.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            complement(14555..14668)
                     /gene="rpmJ"
                     /locus_tag="WGN53_000299"
     CDS             complement(14555..14668)
                     /gene="rpmJ"
                     /locus_tag="WGN53_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ0139336.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            complement(14689..14907)
                     /gene="infA"
                     /locus_tag="WGN53_000300"
     CDS             complement(14689..14907)
                     /gene="infA"
                     /locus_tag="WGN53_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ0139337.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            complement(14912..16222)
                     /gene="secY"
                     /locus_tag="WGN53_000301"
     CDS             complement(14912..16222)
                     /gene="secY"
                     /locus_tag="WGN53_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ0139338.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            complement(16234..16668)
                     /gene="rplO"
                     /locus_tag="WGN53_000302"
     CDS             complement(16234..16668)
                     /gene="rplO"
                     /locus_tag="WGN53_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ0139339.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            complement(16670..16855)
                     /gene="rpmD"
                     /locus_tag="WGN53_000303"
     CDS             complement(16670..16855)
                     /gene="rpmD"
                     /locus_tag="WGN53_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ0139340.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            complement(16848..17366)
                     /gene="rpsE"
                     /locus_tag="WGN53_000304"
     CDS             complement(16848..17366)
                     /gene="rpsE"
                     /locus_tag="WGN53_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ0139341.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            complement(17385..17738)
                     /gene="rplR"
                     /locus_tag="WGN53_000305"
     CDS             complement(17385..17738)
                     /gene="rplR"
                     /locus_tag="WGN53_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ0139342.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            complement(17752..18285)
                     /gene="rplF"
                     /locus_tag="WGN53_000306"
     CDS             complement(17752..18285)
                     /gene="rplF"
                     /locus_tag="WGN53_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ0139343.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            complement(18299..18691)
                     /gene="rpsH"
                     /locus_tag="WGN53_000307"
     CDS             complement(18299..18691)
                     /gene="rpsH"
                     /locus_tag="WGN53_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ0139344.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            complement(18707..19012)
                     /gene="rpsN"
                     /locus_tag="WGN53_000308"
     CDS             complement(18707..19012)
                     /gene="rpsN"
                     /locus_tag="WGN53_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ0139345.1"
                     /translation="MAKKALINRNLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            complement(19015..19554)
                     /gene="rplE"
                     /locus_tag="WGN53_000309"
     CDS             complement(19015..19554)
                     /gene="rplE"
                     /locus_tag="WGN53_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ0139346.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            complement(19564..19887)
                     /gene="rplX"
                     /locus_tag="WGN53_000310"
     CDS             complement(19564..19887)
                     /gene="rplX"
                     /locus_tag="WGN53_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ0139347.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            complement(19899..20267)
                     /gene="rplN"
                     /locus_tag="WGN53_000311"
     CDS             complement(19899..20267)
                     /gene="rplN"
                     /locus_tag="WGN53_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ0139348.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(20489..20752)
                     /gene="rpsQ"
                     /locus_tag="WGN53_000312"
     CDS             complement(20489..20752)
                     /gene="rpsQ"
                     /locus_tag="WGN53_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ0139349.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            complement(20752..20943)
                     /gene="rpmC"
                     /locus_tag="WGN53_000313"
     CDS             complement(20752..20943)
                     /gene="rpmC"
                     /locus_tag="WGN53_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ0139350.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            complement(20943..21359)
                     /gene="rplP"
                     /locus_tag="WGN53_000314"
     CDS             complement(20943..21359)
                     /gene="rplP"
                     /locus_tag="WGN53_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ0139351.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            complement(21343..22035)
                     /gene="rpsC"
                     /locus_tag="WGN53_000315"
     CDS             complement(21343..22035)
                     /gene="rpsC"
                     /locus_tag="WGN53_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ0139352.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            complement(22045..22374)
                     /gene="rplV"
                     /locus_tag="WGN53_000316"
     CDS             complement(22045..22374)
                     /gene="rplV"
                     /locus_tag="WGN53_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ0139353.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            complement(22383..22661)
                     /gene="rpsS"
                     /locus_tag="WGN53_000317"
     CDS             complement(22383..22661)
                     /gene="rpsS"
                     /locus_tag="WGN53_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ0139354.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            complement(22668..23501)
                     /gene="rplB"
                     /locus_tag="WGN53_000318"
     CDS             complement(22668..23501)
                     /gene="rplB"
                     /locus_tag="WGN53_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ0139355.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            complement(23507..23821)
                     /gene="rplW"
                     /locus_tag="WGN53_000319"
     CDS             complement(23507..23821)
                     /gene="rplW"
                     /locus_tag="WGN53_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ0139356.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANAAKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            complement(23818..24438)
                     /gene="rplD"
                     /locus_tag="WGN53_000320"
     CDS             complement(23818..24438)
                     /gene="rplD"
                     /locus_tag="WGN53_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ0139357.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            complement(24438..25082)
                     /gene="rplC"
                     /locus_tag="WGN53_000321"
     CDS             complement(24438..25082)
                     /gene="rplC"
                     /locus_tag="WGN53_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ0139358.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
BASE COUNT         7003 a         6480 c         5373 g         6618 t
ORIGIN      
        1 ctttatcaag agaacttttt catgttgttt caatatttga gattgtttta agaaacaaaa
       61 ttgacatttg ccttcagcag gcgtttaaag acagaaactg gttatataac agcatacagc
      121 cacagacgaa tcccgcatta aaatatcaag gttgcttttt aagaaatggc acgaaagaat
      181 ctgctgaatt aatcaaggtt gcattatcta aaatacaaaa taatagcggg ggaaagtttg
      241 accacaacca attggttgca ggactaggat tcggtttttg gcggtatctg tttgcaggag
      301 ggaaagatgc ccaatttgat gctacaggga aagtattgat gaaagttttt ccaaaaaagc
      361 ccaaatctac accaagtgtg caatataacc aaaaatggat ttttcgagaa ctttccaata
      421 ttaacaaatt ccgtaatcgt ttggcacatc acgagccgat ttgttttaaa ggtgcaataa
      481 aagataccaa ctacgctaga aatattcacc aatctatttt tgaactgctt aattacatgg
      541 atgtagatac tgcttcagta tttagccatt ttagcgatca agtgattgcg gtctgcgatg
      601 aaatcgacaa actgtaattt tctattattt gtcttaacaa gcagcggagt attcaaaaca
      661 tgaccctcat ccacccgacc gccgtcatcg accccaaagc cgaactcgac tccagcgtca
      721 aagtcggcgc gtacaccgtc atcggcccca acgtccaaat cggcgcgaat accgaaatcg
      781 gcccgcacgc cgtcatcaac ggccacacca gcatcggcga aaacaaccgc attttccaat
      841 ttgccagcct cggcgaaatc ccgcaggaca aaaaataccg cgacgagccg accaagctga
      901 ttatcggcaa cggcaacacc atccgcgaat tcaccacctt taatttaggc acggtaaccg
      961 gcatcggcga aacccgtatc ggcgacgaca actggattat ggcgtactgc cacctcgcgc
     1021 acgactgcgt ggtcggcaac cacaccatct tcgccaacaa cgcctcgctt gccggacacg
     1081 ttaccatcgg cgactacgtc gttttgggcg gctacacgct ggttttccaa ttctgccgca
     1141 tcggcgacta cgccatgacc gcgtttgccg caggcgtaca caaagacgtg ccgccctact
     1201 ttatggcatc gggctaccgc gccgaaccgg cggggctcaa cagcgaaggc atgcgccgca
     1261 acggttttac cgcagagcag atttccgccg tcaaagacgt gtacaaaacc ctctaccatc
     1321 gcggcattcc gtttgaagaa gccaaggcag acattctccg ccacgccgaa acccaagccg
     1381 agcttgccgt attccgagac tttttcgcac aatcggcacg cggcatcatc cgctgaccgt
     1441 acccttttga tgccgtctga aaccccgaca gcgtttcaga cggcatcggt ttttttaaag
     1501 cggtatattg tttgatcgat gcatcatttt gtagatttca gaagtatggc agtcggcagc
     1561 attgatgatt tctgccgccc gccacatccc cattcggaca tttcattgtg acctccgccg
     1621 ctgcttttaa agaattgcaa acaatttccg cagcttgaac cggcctgttt tcggcgaaaa
     1681 aaaccaaaag tatttttcaa aagaacatat tgtcttattg gatattctgt gctgaaatta
     1741 ctggacacta caaaagtggt gttttaaaaa taaaacagac aaaggagaaa gccatgcaag
     1801 tgtttttaga caatcccaaa gccttttttg aaaccgtcag cggctgggtt tggggacctg
     1861 ttatgttgat gctgctggtc ggcacgggta ttttgctgac tgttttatta aaaggtttgc
     1921 agttcacgat gttgggttat gcgctgaaac aggcgtttgt gccgccaaag aagcataaaa
     1981 gcggcgaagg ccacgaaggc gatatttccc atttcgcggc gttgatgacc gcgctgtccg
     2041 ccaccatcgg tacgggtaac atcgccggcg tggcgactgc ggtggtaacc ggcggcccgg
     2101 gcgcggtatt ttggatgtgg atgaccgcca ttttcggcat ggccaccaaa tacggcgaag
     2161 gcgtgttggc ggtgaaatac cgcgtcaaca attccaaagg cgaaatgtcc ggcggcccga
     2221 tgtattacat cgaaaaaggc ttgggcaaaa actggaaatg gatggcgttg gcgttcgctt
     2281 tattcggcac attcgcttcc ttcggtatcg gcagctcggt gcagtccaac tcggttgcac
     2341 aggcggtgca aaccagcttc ggtatcgaac ctgcctatac cgacattacg ttgaccgttc
     2401 tgactgccgt tgtcgtttta ggtggtatta aaggcatcgc caaagccgct tctttcatcg
     2461 tgcctgctat ggcggtgttt tatgtgttgg gcggtctttc cattatcgcg attaattccg
     2521 atgcactgat gcctgccgtc aagctaattt tctccgatgc gttcagcgcg caggcagtgg
     2581 cgggcggcgc tatcggtacg gtcatccgct acggcgtggc gcgcggcgtg ttctccaatg
     2641 aggcgggtat gggttctgcg cctattgccg ccgcagccgc gaaaaccgac catcccgtcc
     2701 gtcaggcctt ggtttctatg accggtacgt ttttggatac catcgtcgtt tgctcgatta
     2761 ccggcatcgt gttggtaatg ggactgctcg gcgcgggcgg cgagtttgtc aaacctgaag
     2821 tgagcggcgc ggcactgaca accgtcacat tccaaaaaat gctgcccggc atcggcggct
     2881 ggattgttac catcggcctg attttctttg cctactcaac cattttgggc tggtgctatt
     2941 acggcgaaaa atgcgcggtg tatgttttcg gcgagaaatt tgccggtttg taccgcgtgg
     3001 gttatgtttc ttccgttatg ctgggtaccg tgttgagcct tgatttggtg tggctggctt
     3061 cggacacatt caacggcttg atggcattgc ccaacctgat tgcactttta ttgatggcga
     3121 aagtcatcgt caatgaaacg cgcgacttca aacaaaaaat cacaaacggc gaattgccgc
     3181 attaatatcc gaggccgtct gaatccgaat attcagacgg cacggcatat taagtctgtt
     3241 ttgaaaaagt cggagggaga aacatgaaag tgcttgtttt aggtgcgggt gttgccggcg
     3301 tgtcttccgc gtggtatctg gcagaggccg gacatgaagt aacggtcatc gaccgcgcca
     3361 agggtgtggc gatggaaacc agttttgcca atgcaggcca gctttcttac ggctatacca
     3421 cgccttgggc tgcacccggt attccgacca aagcactgaa atggctgttt aaaagccatc
     3481 cgcctttgct gtttcgcccc gacggcagcc tgtatcaaat cgaatggctg tggcggatgc
     3541 tgcaaaactg cacggcagcg cactatcaaa ccaataaaga gcgcatggtc aggatttccg
     3601 aatatagccg tgaaatgttc cgccgttttg aagcgcaaac cggcatgaat tttgaggaac
     3661 gcaaaaaagg gacgttgcag attttccgcc aaaccaaaga agtcgaagcg gcagaacaag
     3721 acattgccgt tttggaacgc tacggcgtgc cgtaccgccg tctgaaaccc gaagaatgtg
     3781 cagaatttga gcctgcgctg gcacgcgtta ccgccaaaat tgccggcggc ctgcacctgc
     3841 ctgcagatgc gaccggcgac tgccacctct tcactgaaaa cctatacaaa ttgtgtcagg
     3901 aaaagggcgt acagtttcat ttcaaccaaa ccatcagccg catcgaccat aacgggctgc
     3961 gcatcaaagc cgttgaaacc gaaacagggc ggtttgaagc agatgccgtt gtctgtgcgc
     4021 tcggctgctt cagtaggact gtgttggcgc agttagacct cgatctgccg atttatcccg
     4081 tcaaaggcta ttccttgacc ctgccggtca ccaattcaga cggcgcgccg gtgtccaccg
     4141 ttttggatga aagctacaaa gtcgccatca cgcgctttga caacagaatc cgcgtcggcg
     4201 gcatggcaga attgtcgggc tacgaaatca aactgcccga aaaacgccgc gaaaccttgg
     4261 ctttggtcgt caacgatttg ttcccggaag gcggcgattt gagccaagca ttgttctgga
     4321 gcggcctcag accgatgacg cccgacagca cgccgttaat cggccgcacc cgctttgaaa
     4381 acttattctt gaatacaggc cacggcactt tgggctggac catgtcgctg ggttcggcaa
     4441 aattaaccgc cgatatcgtg agcggcaaag acaccgaaat ccgaagcgac gatttgagcc
     4501 tgtcgcgcta tcaggcttaa gctggaaagc gataaggttt agcgacattt caaatttaaa
     4561 ggccgtctga aaatcatttt tcagacggcc ttttgtttca aaattcgggt atccaatcaa
     4621 taaaataaat aataactatt atcatacgca ttaaaaagtg ttagtctgaa cctgtcccag
     4681 cttttagcgc aggttttccc atgcccgatt tttcgatgtc caatttggcc gttgcctttt
     4741 ccattacgtt ggctgccggt ttgtttaccg tattaggcag cggcttggtg atgttttcca
     4801 aaacgcccaa tccgcgcgtg ttgtcgtttg gtttggcatt tgccggcggt gcgatggtgt
     4861 atgtttccct gacggagatt ttcagtaagt ccagcgaggc gttcgctgaa atttatgata
     4921 aagaccacgc gtttgcggcg gcgaccatgg catttttggc agggatgggc ggcattgcgc
     4981 tgattgaccg tctggtgccg aacccgcatg aaactttaga cgcgcaagac ccgtcgtttc
     5041 aagaaagcaa acgccgccat atcgcgcgag tcggcatgat ggcggcgttt gcgattactg
     5101 cgcacaattt ccccgaaggc ttggcgacgt tttttgccac attggaaaat ccagcagtcg
     5161 ggatgccttt ggccttggcg attgccatcc ataatattcc ggagggcatt tccatcgccg
     5221 cgccggttta ttttgccacc cgcagccgta agaaaacggt gtgggcgtgt ctgctatccg
     5281 gcttggccga gccgttgggg gcggctttgg gctatttggt tttgcagccg tttttgtcgc
     5341 ctgccgtgtt tggttcggta ttcggcgtga tagccggtgt gatggtgttt ttggcgttgg
     5401 acgagctgct gccggctgcc aaacgctatt cagacggcca tgaaaccgtt tacggcctga
     5461 caatgggcat ggcggtgatt gccgtcagcc tggtattgtt ccatttttaa aacctgtatg
     5521 ccaaaggccg tctgaaatga ttcagacggc ctttttgcgt tataatccgc acatcaaaaa
     5581 taaccttaat ttcacaaagt tccatcatgt tagacaaata ctcccccgcc gaaatcgaat
     5641 ccaaacatta tcaaaactgg gaagagcagg gctatttcca acctgatatg gatttgacca
     5701 aaccgtcttt ttccatccaa ctgccgccgc ccaatgtaac cggcacgctg cacatgggtc
     5761 atgccttcaa ccaaaccatt atggacggcc tgacccgcta ctaccgtatg aaaggctgca
     5821 acaccgcctg gattcccggc accgaccacg cgggcatcgc cacgcaaatc gtggtcgagc
     5881 gtcagcttgc cgcgcaaaac gtgtcccgtc atgacttggg tcgtgaaaaa ttcttggaaa
     5941 aagtgtggga atggaaagaa gtttccggcg gcacgattac gcagcagatg cgccgtgtgg
     6001 gctgttctgc cgactggacg cgcgagtatt tcacaatgga cgatgtacgc gccgaaaccg
     6061 taaccgaagt gttcgtacgc ctgtatgaac agggcttgat ttatcgcggc aaacgcttgg
     6121 tgaactggga tccggtattg ggtaccgcag tgtctgattt ggaagtggaa agcgtggaag
     6181 aacaaggctc tatgtggcac atccgctatc cgctggcgga caatcctgcc gaagccgtca
     6241 tcgtcgccac cacccgtccc gaaacgctgt tgggcgacgt tgccgttgcc gtcaatcccg
     6301 aagacgaacg ttatacccac ttaatcggca aagaattaat cctgccgctg accggccgca
     6361 ccattcccgt gattgccgac gaatacgttg aaaaagactt cggcaccggc tgcgtgaaaa
     6421 tcacgcctgc gcacgacttc aacgactacg aagtcggcaa acgccacgat acgcgcttgg
     6481 ttaatgtgtt cgacttggaa gccaaagtat tggcaaacgc cgaagtgttc aacttcaaag
     6541 gcgaagcgca accaagcttt gccctgcctg aaaaatacgc aggcttggac cgctttgccg
     6601 cgcgcaaaca aatggttgcc gatttgcagg aacaaggttt cttggtcgaa atcaaagcgc
     6661 acacgctgat gacgccgaaa ggcgaccgta ccggttccgt gattgagccg atgctgacca
     6721 gccaatggtt tgtcgccatg tccgccacgc caaacggcgg cgagcctgac agcgaattca
     6781 aaggcttaag ccttgctgac aaagccaaaa aagccgtcga cagcggcgcg gtgcgcttta
     6841 tccccgaaaa ctgggtcaac acctacaacc aatggatgaa caacatccaa gactggtgca
     6901 tctcgcgcca actgtggtgg gggcatcaaa tccctgcatg gtacgacaac gaaggcaatg
     6961 tgtacgttgc ccgcaatcag gaagaagccg aaaaacaagc cggcaaaaca ggcttgaccc
     7021 gagaagaaga cgtattggac acatggttct cctccgcact cgtgccgttc tccacgctcg
     7081 gctggccgtc tgaaaccgac gaactcaaag ccttcctgcc gtccaacgtc ttggttaccg
     7141 gctacgaaat catcttcttc tgggtggcgc gcatgattat gatgaccacc cactttaccg
     7201 gcaaagtacc gtttaaagac gtgtacatcc acggcatcgt gcgcgaccac gaaggcaaaa
     7261 aaatgtccaa atccgaaggc aacgtcattg accctgtgga tttgatcgac ggcatcgact
     7321 tggaaaaact gctggtgaaa cgtaccaccg gtctgcgcaa acccgaaacc gcgccgaaag
     7381 tggaagaagc ctcgagaaaa ctcttccccg aaggcattcc gagtatgggc gcggacgctt
     7441 tgcgcttcac catggcgagc tacgccagct tggggcgcag cgtgaacttc gacttcaaac
     7501 gcgccgaagg ctaccgcaac ttctgcaaca aaatctggaa cgccaccaac ttcgtcttga
     7561 tgaacaccga aaaccaagac tgcggctacg gcgcaaccgc cactgagccg cgcggctatt
     7621 ccttccccga tatgtggatt gtagaccgtc tgaatcagac catcgagcaa gtaacgcaag
     7681 cctacgaaac ctaccgcttt gatttggcag cggaaaccct gtacagcttc atgtggaacg
     7741 actattgcga ctggtatttg gaacttgcca aagtgcagct tcaaaccggc tgcgccagcc
     7801 gccaacgcgc cacacgccat accttgttgc gcgtactcga agccgccctg cgcctgctgc
     7861 acccgattat cccgttcatc accgaagaat tgtggcaaac cgtcgctcct atgtgcgacg
     7921 cgaaaaccgc cgacagcatc atgctcgccc gcttccccga agccgacggc ggcgatattg
     7981 ttcagacggc attcgagcaa atgaccgtgt tgcaggattt gattggcgca gtccgcaacc
     8041 tgcgcggcga aatgggcatc cagcccaatg tgaaagcccc gctgtttgtc gaaagcacgg
     8101 acgacttggc ggactacctc aaatacctgc cgatgatgac ccgcctgatc gaagcacagc
     8161 aagtcgccgc cctacccgaa agcgaagacg cgcccgtcgc cgtctgcaac ggcgcgcgcc
     8221 tgatgttgaa agtcgaaatc gacaaagctg cggaaaccgc ccgtttgagc aaagaagccg
     8281 agaagctgca aaaagccttg gacaaactca acgccaaact ctccaaaccc ggctacaccg
     8341 aaaaagcccc tgcgcatctg gtggaaaaag acaaagccga tttggcggaa ttggaagaca
     8401 aaatggcaaa agttcaaaat cagttggcga agttgaaaga ctgatttgat tgaataaaca
     8461 aggccgtctg aaaaatgttt ttcagacggc ctcttttata gtggattaac aaaaatcagg
     8521 acaaggcgac gaagccgcag acagtataaa tagtacggca aggcgaggca acgccgtact
     8581 ggtttttgtt aatccactat acatggcatt gtatcggcac tgatgaaaac attcacagtg
     8641 atgcaaaagt cgaatatctt caggacggca gtttccgtgg cgatgctata ctcaaaatcg
     8701 atgatgacgg caatatcttg gcataccgtg ttgtcggctc agggaaatgg cgttttgaaa
     8761 acaatgcctg cgcctagtcg cggataaaac ttaccccgtg aagctgcgac tgcataatcg
     8821 ccgccttcat cgccgacaac gccttcggac ggacaaaatt gcggcggtac gccaatacga
     8881 cccgtcggct tggcggcgta ccctcaaacg gaataatgct gaacagcata tgatcgttct
     8941 cggtcagtgc ggttgccggc aacacgctga ttgccaaacc gctggcgacc atatggcgga
     9001 ttgtattgat cgagctgccc tgcaatgtat tggtcaaccc ctgtatgcgt tgtttcgccg
     9061 ccaattcgga acagcttgag agtacctgat cccgcataca gttgccttcc gtcagcagca
     9121 aaacctgctc ctcacccagc atccggggcg aaacggcatc cagttcctca aatgaatgcc
     9181 ctttcgggac aatcacgaaa aacggttcgt catacaaggg ttcggtaaca atgcccggct
     9241 cttgaaacgg ttcggcaacg ataatcgcat caacgtcccc gcgtttgagc gactcggtca
     9301 aagtatgcgt gtaattctct tccaacatca aaggcatttt cggtgcagta cggcgcaacg
     9361 agacaatcag tttcggcagc aggtacggcg caaccgtaaa aatcagcccg agtttgaacg
     9421 caccctccag ctcgttttgt tcttcatttg ccaaatgcct gataagctct gcctcttcca
     9481 acaccttacg cgcctgtgca acgatacgtt cccccgcctc ggtcgtaata atatcgttgc
     9541 tgctccggtc aaacaaagag acggcaagct cttcttccaa tttcttaatg gcaatagaca
     9601 aagtgggctg actgacaaaa caacgccgcg ccgccctgcc gaaatgacgt tcttgggcga
     9661 ctgcgacgat gtaccgcaat tcggttaagg tcatgtccta tacctttttc tgttccggca
     9721 aagtaatgtt caactcaagc acatccatac catcctgttt ttcctgggaa atacggatat
     9781 tgtctaatga aacattcaca tatttggaca ggacttccat caactcttta cgtaaagtcg
     9841 gcaggtaatc cggagcctga ccttcttggg cgcgctcttg ggcaatgatg atttgaaggc
     9901 ggtcgcgggc aacggttgcc gttttctgct ttctgccgaa taaaaattcg attaatgaca
     9961 tatcttatcc tccaaacaga cgtttgaaga agcttttttt ctcagcttcc aagaaacgca
    10021 tttcacggtt ctcgcccaaa agacgggcaa taacgtcctt atatgcctcg gaagccgcca
    10081 cgctgtcctg atggatgacc ggttctccgg aattggatgc ctgcaagacg ttttgggatt
    10141 caggaatcac acccagcaaa ggaatatgca gaatatcgca aatatcctgt acagacagca
    10201 tttcgccttt tgccacacgt tcgggagaat aacgcgtaat caacagatgt tctttaaccg
    10261 aaccgccttg ctctgcctta tgggatttgc tttgcaaaat tcccaaaatc ctgtcggagt
    10321 cacgcacact ggaaacctca ggattggtcg ttacaatggc ttcatcagca aaatacaacg
    10381 ccatcaatgc accctgctca ataccggcag gagagtcgca aataatatac tcaaagccca
    10441 ttttcttgcc ggacagctcc tgcatcactt tttctacgcc ctcgcgtgtc aaagcgtctt
    10501 tatcccgagt ctgggaagcc ggcaaaataa acaggttttc acaattttta tctttaatca
    10561 aagcttggtt gagcgtcgcc tccccctgaa tgacattgat caggtcataa acgacacgac
    10621 gctcgcaacc cataatgagg tcgaggttac gcaaacccac atcaaaatca attaccgcag
    10681 ttttatatcc gcgtaatgcc aaacctgtcg caatactggc actggtagtc gttttaccga
    10741 caccgccctt acctgaagtt actacaataa tttttgccac aatatttcct ttttaaatat
    10801 caaacaatta ctctgagcca attgcactga taaccaatcg gttatcctgc aacaatatct
    10861 gtaccggctg cttgtgcaga tggttcggca aatcctgttc aaaattacgg taaatacccg
    10921 ccacagaaac cagttctgcc tgcatggagt ggataaatat gcgggcagaa gtatcaccct
    10981 tggcaccggc caaagcacgc cccctcatcg gcgcataaat atgtatattg ccatccgcaa
    11041 tcaattccgc cccctggctg accgcccccg taacaatcaa atcgccatct tcggcataaa
    11101 cctgctgacc ggtacggaca ggggatgtaa tcaatactgt tttccttgct ttctgaccat
    11161 cctccgtgtt ctgcacctca acctgaccca gttctttaac attttccgaa tgagacagac
    11221 aaaacagcaa atgatacttc atagccgcag cagcccaacg ttcattagaa tgcttcagac
    11281 ccaaaatttg cataccatgc cttgaaaaca acgaaaccaa tgcagcaaga tccaaagact
    11341 cgggataatc aaactcttga acatccagca caaatggaac aacaccagac tcttgaaact
    11401 tcttgcccaa tttgaccagc acatcttcca aatcaaacaa gtctgacgta tgcaaagata
    11461 tagacaatac gtccatcttt gtcgacttta tatcgaaagc attcattata taaaccatca
    11521 tagaattttt taatgatgta agtttaccgc gcaaacctta gctattcaat taatatttta
    11581 aacatgatag ccaataccct attacctatc agatattgca aaaataacgc cacctgcttt
    11641 tcggcagatg gcgttatatg actaaaatta ctcagcagcc ggtttgtcaa ccaattcaac
    11701 cagtgccaga ggtgcattat cacctttacg gaatccgtat ttcaacaccc gaacataacc
    11761 accgttacga gcagtaaaac gaggacccaa atcgccaaac agttttacta caacatcacg
    11821 gtcgcgagta cggtcaaatg ccaaacggcg gtttgccaat gacggctttt tacccaatgt
    11881 aatcaacggc tctactacac ggcgcaattc cttggcctta ggcagagttg ttacaatagt
    11941 ttcgtgagtc aataatgaat tcgccatatt acgcagcatt gcagcacgat gactgctggt
    12001 acggtttaat ttgcgattgc cattacgatg acgcatatca attatccttt aattcttcat
    12061 taaggctttt ccaagcctac aggtggccat gcttccaact tagaacccag tgtcaaacct
    12121 ttagatgcca atacttcctt aatctcattc aaagatttac gtcccaaatt cggcgtttta
    12181 agaagctcgg tttcagtgcg ttgaatcaaa tcgccaatat aataaatatc ctcagctttc
    12241 aaacaattag ctgaacgtac tgtcaattcc agatcatcca ccggacgcaa aagaacaggg
    12301 tcgataggag gtgctttttc ttcaacctcc tccacaggcg taccctgcaa atcagcaaaa
    12361 atagacatct gatcaatcaa aatacgtgcc gcactgcgta cagcttcctc aggatcaata
    12421 gaaccgtcgg tttcgatatc caaaaccaac ttatcaagat ccgtccgctg ctctacacgt
    12481 gcaggttcaa cctcaaagct aacacggctg atgggcgaaa agctcgcatc caactggatt
    12541 gcaccaatct gacggttctc atcacgaact acctgacgac ctgaaacaga ttgataacca
    12601 cgaccttgct ctactttaat ttccatctca atttgaccgt tatcagccaa atgacaaatg
    12661 acatgaccag gattcagaat ttctacatca tgcggcaact caatatcacc ggcagatacg
    12721 acacctgaac ctgatttctt caacacaagt tgaacttggc tacgaccatg gagtttaaac
    12781 acaatacctt taatattcag caaaatgtca acaacatctt cctgaatacc atcaacagta
    12841 gaatattcgt gtaatacacc ggcaatagct acttcagtag gagcaaaacc attcatggat
    12901 gacagtaaga tacggcgcaa agcattacct aaggtatgac cgaaaccacg ttcaaatggc
    12961 tgcatagata cttttgcacg agttgcagaa aaagtattta catcaatttg acgaggtttc
    13021 aaaaattcgg ttgtgctatt ctgcatttaa ctgtccctca ctgagctagc attatttaga
    13081 gtagaactct accaccagct gttcattaat atcaccggtc aattccgagc gatccggcat
    13141 gtttttgaac acaccctcaa gtttatccgc atctacagaa acccaacccg gcaagccgat
    13201 ttgagttgcc aaacccaatg cttcttgaat acgtacctgt tttttggctt tttcacgaac
    13261 tgagacaaca tcaccagctt tcacttggaa agaaggaata ttgacaactt gtccattcac
    13321 aactatcgcc ttatgagaaa caagctgtct tgcttcagct cgggtagaac cgaaacccat
    13381 acgataaacg acattatcca aacgagattc cagcaactgc aacagcaact cgccggtaga
    13441 acctttacga cgatcagctt ctgcgaaata acgacggaac tgacgttcta atacgccata
    13501 aatacggcgg attttttgtt tttcacgcaa ctgcaaacca tagtctgaca aacgcggttt
    13561 ttttgcacca tgctgaccag gagcggaatc aattttacat ttagaatcca aagagcggcg
    13621 cgcactcttc aaaaacaaat ccgtaccttc gcgacgtgcc aacttacatt tagggccaat
    13681 ataacgtgcc atgtttcaaa tcactccaat attaaatacg acgtttttta ggcggacggc
    13741 aaccgttatg aggcaacggg gtaacgtcag taatgctggt aatcttgaaa ccaagagcat
    13801 tcaaagcacg tacagaggat tcgcgacctg gacctggacc tttaatacga acctctaaat
    13861 ttttaacgcc atactcttgg gcaactttac cagctgcttc tgctgcaact tgtgctgcaa
    13921 atggtgtact tttacgagaa cctttaaaac cagcgccgcc agaggtagcc caagacaacg
    13981 cattgccttg acggtcagtg attgtaatga tggtattgtt gaaagatgca tgaacgtgca
    14041 caataccctc actcacggtt ttacgtactt ttttacgtac acgtgaagct gtgtttgctt
    14101 tagccattaa taaaattcct taaaatttat ttcttaccag caatcgcttt acgcggacct
    14161 ttgcgggtac gcgcatttgt acgagtgcgt tgaccgcggc atggtaagcc gcgacgatga
    14221 cggaagccac gatagcagcc catgtccatc aatcgcttga tactcatagt tacctcacga
    14281 cgcaaatcac cttctacttc atacttggca acttggtcac gcaaagcatc taattgagtc
    14341 tcgtccaaat cttttgcttt agtatcaggc gcaatatttg cagcctcaca aatcaattta
    14401 gcacgagtag caccaatacc gtaaatagcc tgaagaccaa ttacgatgtg tgcgttatta
    14461 gggatattta cccctgcaat acgagccata ttttttcctt tttagggcaa agtgtgtcac
    14521 tatatcacag aatcacatta aaagaaatat tccattaacc ttgacgctgt ttgtgacgaa
    14581 gatcagtaca aattacacga actacacgat ttcgacgaat aatcttgcaa tttcggcaaa
    14641 ttttcttaac agatggttgt acacgcattt tattttcctt ttattggttt atcttgctct
    14701 gaaaacgatt cgagccctag ttagatcata aggtgtcagc tctactgtga ccttatctcc
    14761 cggagaaata cgaatgtaat gcatccgcat cttcccagaa atatgaccca atacaatatg
    14821 gtcattctca agttttactt taaatgttgc attaggtaaa gtttcaagaa tttcaccttg
    14881 catttggata gtatcttctt tcgccataat tctatttccg agaaaatgat ttcatttctg
    14941 aacgagtcat taacttatca tactgttgag taagcctata cgaatttatt tgtgtactaa
    15001 aatccatcgt tacaacaact agaatcaaca aagacgtgcc acccaaataa aaaggtacat
    15061 ttaaaaccgt agttaagaac tctggaatta aacaaatagt tgtaatataa agagctccaa
    15121 acaatgtcaa acgtaataca actttttcta aatacctaga ggtctgctca ccaggtctaa
    15181 tcccaggaac aaaagcacca ctctttttta aattctctgc catttcttta gggctaaaaa
    15241 ccaaagccgt ataaaaataa caaaagaaaa taactgtcgc tgcaaataaa gccatataca
    15301 gcaattgacc gtgttgtaac aatccagcta ttttgtgcaa aacactattt gtatcagccg
    15361 aaccaaacca acctaaaaga gtagatggaa atagaataat actggaagca aaaattgggg
    15421 gaataacacc agccatattc aacttgaaag gcatatgcgt attttgacta cccgccctac
    15481 cattaaactg gcgttttgca taatgaatag gaatcttccg ctgtgcactt tcaaagtata
    15541 caaccaaata aattaataat aaggcaccaa atacaataaa caacgccgta agcatgctca
    15601 tagaaccttg gttcgtcagt gtaaccagct ttgcaatacc cgaaggaata cctgaagcaa
    15661 tacctgccgt aatgattaaa gaaataccgt tcccgatacc cctttcagta atttgctccc
    15721 caagccacat aagaaacatg gttcccgtta ccaaagaaac taccgtggaa acatgaaact
    15781 caaatgaact tgttacaaca attccttgct gaaatacgaa agatgcaaca cctagacttt
    15841 gaagaattgc taacaaaaca gtaccatacc tagtatattt cgtaattacc tttctaccag
    15901 cctccccttc tttttttaaa gccttcaatg atggcaaaat ttcagaagcg agctgtacaa
    15961 taatagaagc tgaaatatat ggcataattc ctattgcaaa tatactaaag cgctctaacg
    16021 acccaccgga aaacatattc aatattccca ggatgccgtt tccagcgctt tcgtataatt
    16081 tagctaaagc aacagcatca actccaggta cgggtatatg ggcaccaatt cgaaaaacaa
    16141 tcaatgctcc aaatagaaac aaaagacgtt tcttaagatc tccaaatttg gatgaacctg
    16201 atgacgtttg ttgattagcc acaatattaa accttacatt tcaatcttac caccaacagc
    16261 ctcgatagca gctctcgcac ctttggtaac tttaataccc ttcaaagcaa ctgccttaga
    16321 aatttcacca gaagcaataa ctttaacatt agagactgta gatgcaatca gacccgcttg
    16381 ctttaagacc aaaatatcaa tctcattaac agcaattgat tccagttcac ttaaacgaag
    16441 ctgtgcatta gctgatgctg ttaaagattt aaaacctctt ttagggaggc gtcgttgcaa
    16501 gggcatttga ccaccctcga aacccacctt atgaaaccca ccagaccgac tcttttgacc
    16561 tttatgacca cgaccacccg ttttgccaag accactacca ataccgcgtc caacacgacg
    16621 accagcatgc gtagcaccaa cagcaggttg aattgtattc aaaaacatat caagactcca
    16681 ctttcaacaa gtagctgatt ttattaatca taccacggtt ttcaggggta tctaaaacct
    16741 ctaccgtatg ctcgcgacga cgcaaaccta aaccgcgtgc acatgcacga tgagattcaa
    16801 ttgtaccaat caggctttta accaatgtaa ccctaatctt tttttgttca gccatggtta
    16861 actcccaaaa tgtcttccac tgtcaagcca cgtttggctg cgatatcagc aggagtatgc
    16921 aacttagaca aaccatctaa tgttgcacgt acgatattat atgggttagt agatccgtgc
    16981 actttggcgg agatattatg aatgcccata gcatcaaaaa ccaaacgcat aggtccaccg
    17041 gcttttacgc cactaccctc tttagcaggc tgcataaata ctttagtagc accatgtcga
    17101 ccaataacct catgatgaat cgtaccattt tttaatggta ccttaatcat agagcgtcgt
    17161 gcttgatcca tcgccttttg gactgcaaca ggcacttctt ttgacttacc tttacccata
    17221 ccaatacgac catctccatc accaacaaca gttagcgcag agaaagccat aatgcgacca
    17281 cctttaacta ctttggttac acggttaact gcgaccatct tttcaatcag accgtcaccg
    17341 cgttcttcaa tttcatgttt tgccatctga aagtctccaa atatttagaa gcttaaacca
    17401 ttttcacgag cagcttcagc caaagccttc acacgaccgt gatattggaa acctgaacga
    17461 tcaaaagcaa ccttttctac acctgctgct ttagccttct cagcgatacg tttaccaact
    17521 attgcagctg cttcaacatt gcttccagat ttcagactac cgcgcacctc agcttccaat
    17581 gtagaggctt gagccaatac tttatcacct tcagcactaa ttacttgagc ataaatatga
    17641 ttattgcttc ggaacacaca taatcttacc attttcaagt ccgcaatacg agcacgggtt
    17701 ttgcgtgcac gacggagtcg ggttgtatgt ttatccatta gtgaacctca attatttttt
    17761 cttggcttct ttcattacca ctacttctcc tacatagcga acacctttac ctttataagg
    17821 ctcaggagca cggaacgcac gaatctcagc agcaacttga ccaacaactt gtttatccga
    17881 gccggttaaa acaatctctg tttggctagg agtttgaacg gagacacctt caggcatttc
    17941 atatacgatc ggatgagaaa aacccaaaga cagattcaag attttacctt gtgcttgagc
    18001 acgataaccc acacctatca attgcaatct tttctcaaaa ccttctgaaa cacctttaac
    18061 catattgctg actaatgcgc gagcagtacc agacattgca tttgcttgtt tactgctgtt
    18121 attcgcaaca aaagccaatt tgccatcatt aaattcaatg gctacatcag aatgcaaagg
    18181 aaaagacaat tcaccgttct tacccttaat aactaatgcc tctgttccaa attttacttc
    18241 tacaccagcg ggaacagtca ctgggttttt tgcgacacgt gacatttctt ttcctccact
    18301 aggctacaat gcataacaac tcaccaccaa caccttgaga acgtgcttta cgatcagtca
    18361 ttacaccttt agaagtacta acaatagcaa tacccaagcc attcataaca cttggaatct
    18421 cactagacgc tttataaata cgcaaaccgg ggcgagatac acgcttgatt tgttcaatta
    18481 caggacgacc tgcatagtat tttaattgaa tttccaatat agactttacg tcagatgaaa
    18541 ctgcgaagtc ctcaatatat ccttcttctt tcaatacctt tgcaatagca cactttaatt
    18601 ttgaagaagg cattgcaacc gctgctttat tagcacgttg cgcattgcgg atacgagtca
    18661 acatatcgga aataggatca tgcatactca ttcttaatta ctcctattac cagctggctt
    18721 taacaacacc cggaatttcg ccacgcatgg cgatttcacg gattttaata cgacccaaac
    18781 caaatttacg gaaagtacca cgagggcgac ctgtcaaagc acaacgacga cgttgacgca
    18841 caggtgccgc attacgagga atggattgaa acctcaaacg agcctcaaaa cgctcttcct
    18901 cagttgcatt cgaatcattg attaccgctt taattgccgc gcgtttagcc gcatattttt
    18961 tagccaaagc ttgacgtttc agattacgat taataagtgc tttcttagcc atgattatcc
    19021 tttgaacgga aatttaaaca atgacaataa agctttcgct tcctcatcgg tttttgctgt
    19081 agtagtaata gtaatattca aaccacgcaa agcatcaatt ttatcgtatt caatttccgg
    19141 aaaaataatt tgctcacgaa cacccatatt gtaattgcca cggccatcaa atgatttacc
    19201 gctcacacca cggaagtcac gtacgcgagg taatgcaata gtaatcaaac gatccaagaa
    19261 ttcaaacatt tgatcacgac gcaatgttac tttgcaacca accggatagt tatcacggat
    19321 tttaaaacct gcgatagatt tacgggcaac agtaacaacc ggtttttgac cggcaatttt
    19381 ctctaaatcg gaaacagcat gttccataac ttttttgtca gcaacagcct cacccacacc
    19441 catattcaag gtgatttttt caatacgcgg gacttccatt actgatttgt aaccaaattg
    19501 tttaaccaat tcaggaacaa ctgtctcttt ataaaactct ctcaaccgag ccatgttatc
    19561 tccttatgcc ccaatgatag agccatttga tttgaagaaa cgaacgcgtt taactttgcc
    19621 ttcattttca atcagcttaa taccaacacg gtccgcttta ttagtttccg gattcaggat
    19681 tgcgatatta gaaatatcca aaggcatttc tttagtaata ataccgccct caatgccacg
    19741 cattggatta ggtttttgat ggcgttttac aacattaacg ccctcaacaa caactttatc
    19801 acccaacact cgaactactt gaccctgctt acctttatcc ttaccagcaa ttactacaac
    19861 cctatcgcct ttaatgattt tattcatcgt gccattcctt ataatacttc aggtgccaat
    19921 gaaacgattt tcataaatcg ctcagtacgc aattcacggg ttaccggacc aaagatacga
    19981 gtacccaaag gttcaagttt attattcagt aacacggcgg cattgttatc gaatttaatt
    20041 aacgcaccat caggacgacg tacaccctta gcagtacgaa caactaccgc attatataca
    20101 tcgccttttt tgacacggcc acgcggagcc gcatctttaa ctgccacttt aataatatcg
    20161 ccaacagaag cgtagcgacg cttagatccg cccaatacct tgatacacat tacgcgacgc
    20221 gcaccagagt tatcagccac atctaagatg gtctgcattt gaatcatgtt attaccttta
    20281 aattaaccaa cttaatttac cactttcaga taacttacag caaagccgct gcttattaaa
    20341 tgaaaccttg cggttcaagt aaaccagtct tggaccccga aagggaagaa acttccagaa
    20401 gaaactgaaa gacaggaaac gaagtttaca cgcaaattaa atttgctgca atacttcgtt
    20461 tcccattcca agcacgttgc tttaattctt aaatagaacg tgctttctca accagctcac
    20521 tgacaaccca agatttagtt tttgacaatg gacgggattc cgatataaca accacatcac
    20581 caattccata ttgattattt tcatcatggg catggatttt agtagataac cgaataatct
    20641 taccatacag cggatgtttt actttacgct caaccaatac tgttacggtt ttatccattt
    20701 tgtcgcttac tactttgcct tgcaaagtac gaacattttt agtttcgctc attacttagc
    20761 acctttttca gttaaaacgg ttttaatacg agcaatatcg cgacgtacac gtttcaattc
    20821 acttggtttg cctaattgac cggtagcgtt ttgcatacgt aagccaaact gagctttcaa
    20881 caagtccaac aaatctgcat tcaactgctc aacggatttg tctttcaatt catttgcttt
    20941 cattattgac ccacctgtct tactacaaag gttgtaggaa taggcaattt ggcagcagcc
    21001 aactcgaatg cttcacgagc cagttcctct ggaacgccat ccatttcata caacacttta
    21061 cctggtttaa tttcggcaat gtaatattcc acgttacctt taccgccacc catacgaact
    21121 tgaataggct tttcagtaat cggtttatca gggaatacac gaatccaaat acgaccacca
    21181 cgtttgatat gacgggtcat tgcacgacga gcagcttcga tttgacgggc agtcaaacga
    21241 ccacgaccta cggctttcaa gccgaactca ccgaaactta ccttattacc gcgagtagcg
    21301 atgccagtat tgcgaccctt ttgttgctta cggtatttca gtctagttgg ctgcagcatt
    21361 acgtctacct gcctttcttt gtttactctc atgttcaggt ttggaagatt taatattgcc
    21421 ttccgtataa acccaaactt tcagacccaa tacaccatat gtggtgtgcg cttcgctggt
    21481 tgcataatct acatttgcac gtaaagtatg caatggcacg cgaccttcac gataccattc
    21541 gctacgggca atatccgcac cattcagacg gcctgaagtc ataatcttaa tgcctttagc
    21601 accagaacgc attgcatttt gcattgctcg tttcatagca cgacggaatt gaacgcgctt
    21661 ttccaactgc tgggcaatac cgtcagcaat aatttgagca tccaactcag gacggcggat
    21721 ctcttcaata tttacatgaa caggtacacc catcaagact tgcaagtcac gtttcaaaac
    21781 ctcgatatcc tcaccttttt taccgataac cacacccgga cgagcggagt gaatggtaat
    21841 gcgtgcagat tttgcagggc gttcaataac cactcgacca accgaagcat tggccaattt
    21901 ttgacgcaaa taattgcgaa catcgatatc ctgcttcaaa acagtagaaa agtcggtgct
    21961 tttagcaaac cattttgaag cccagtcttt agttaccgcc aggcgaaagc ctgtagggtt
    22021 aatcttttgt cccatagctt ttccttagtt acccactgtc acattgatat gacaagtttg
    22081 tttttcgatg cggttaccgc gacctttggc gcgagcttga aaacgtttca agcttgggcc
    22141 tttgtcaaca aagatagtta ccactttcag ttcatcaatg tccgcaccgt tattgtgctc
    22201 ggcattagca atagctgact ccaatacttt tttaatcagc tcggcacctt ttttaggact
    22261 gaaagccaaa atattcaaag cttgggcaac gtctttacca cgaatcaaat cagctaccaa
    22321 acgagccttt tgagcagaga tacgggcatt tttatgttgt gcatttactc tcatgattca
    22381 ccttatttct ttttagcctt tttatcggcc aagtggcctt taaaggtacg ggtcaatgag
    22441 aattcgccta atttatgacc aaccatattg tcgctgataa acacaggcac atgggtgcgg
    22501 ccgttgtgca cagcaatggt cagaccgata aaatcaggca gaatggtaga acgacgagac
    22561 caggttttaa tcgggcgttt gtcgttgctt gcgcgagcag catctacttt tttcagcaaa
    22621 tgcaggtcta catatgggcc ttttttcaat gaacgagcca tactaaatta acctttattt
    22681 gagtaacggc gacgaacaat catgttatcc gtgcgtttgt tattacgagt gcggtagcct
    22741 ttagcaggag taccccatgg gctgaccggt tcgcgggcct cgcccgtacg gccttcacca
    22801 ccaccatgcg ggtgatcgac aggattcatg acaacaccgc gtacggtcgg acgaataccg
    22861 cgccaacggt tggcaccggc tttaccgatt tttttcaggc tttgctcttc gttaccaact
    22921 tcaccgatgg tcgcacggca atttacgttg attttacgga cttcgccaga gcgcagacgg
    22981 acttgagcgt acgcgccttc tttagccagc aataccgcag aagcaccggc agaacgtgcg
    23041 atttgagcac ctttaccggg tttcatttcg atacagtgga tagtcgtacc aacgggaatg
    23101 ttgcggatcg gcagggtgtt gcctactttg atggcagctt cagcgccgga aactaataca
    23161 gcaccggctt gaataccgcg aggagcaatg atgtaacgac gctcgccgtc tgcatagcac
    23221 aacagtgcga tgaaggcagt acggttagga tcgtattcga tacgctctac tttagcagga
    23281 atgccgtctt tgttacgttt aaagtctaca acacggtaat ggtgtttatg accgccgcct
    23341 ttgtgacggg tggtgatatg accattattg ttacggccgg cagtagaatt tttcttttca
    23401 agcaagggtg cataaggtgc acctttgtgc aaaccttctg ttaccacgcg aaccatgccg
    23461 cgacggcctg cagaggtcgg cttcatttta acgattgcca ttttgtttat tccttatctg
    23521 cagctgcagc agcggcttcc aaatccaact cttgaccggc agccaagctt acataagctt
    23581 ttttaacatc gctgcggcgg cccaaagtac gaccaaaacg cttagttttg cctttagttg
    23641 taacggtagt tacagaagca acttgaacac cgaacagcag ctcgacagcc gctttgattt
    23701 caggtttggc tgcatttgcc aaaactttaa acgtcatttg gttgcgtttt tcagccaata
    23761 cgttgctttt ttcagaaacg ataggtgcca aaatcacttg agtcaaacgt tgttgattca
    23821 tacccattgc tcctctaatt gtgcaactgc atctttagtg atgattactt ttttgtaacg
    23881 cagcaagctg taaggatcaa cttgttgagc ttccaaaacc aacacgtttg gcaagttgcg
    23941 tgaagccaag taaacattct cgtcgagctg tttggttaca aacaacactt gctccagacc
    24001 cagatttttc acttgttcgg caaaaacttt ggttttagga gtttcggcag tcaacgcctc
    24061 aatcgcaaac aaacgctcgt cacgagtcaa ttgggacaga atagtcgcca taccggcacg
    24121 gtacattttg cggtttactt tttgagtgaa gttttcgtcg ggtttgttcg ggaacgcgcg
    24181 accaccttta cgccacagcg gagaagaagt cataccggaa cgggcacggc cggtaccttt
    24241 ttgacgccat ggttttttgg ttgagtgttt tacttcggca cgggtttttt gagcgcgatt
    24301 gccggagcgg gcgtttgcca aataggcatt taccagctga tgaaccaacg cttcattgta
    24361 ttcgcgggcg aacaaagcat cagaaacaga cagactgcct gaaacttgtc ctttagcgtc
    24421 aattactttc aattccatta cgcacctact ttcacgctgg gacgaactac aacatcgctg
    24481 ttgaccgcac ctggaacagc acccttaacc aacagcagtt ggcgttctgc atcaacacgg
    24541 acaacttcca atttttgaac agttgctttg gtgttgccgt attggccggc catgcgttta
    24601 ccggggaaca cgcgacccgg gtcttgcgcc ataccgatag agcctggaac gcggtgagag
    24661 cgggagttac cgtgggaagt acgttgggca ccgaagttat gacgtttgat cgtaccggag
    24721 aaacctttac ctttagaggt accggttaca tcgaccagct ggccgacttc aaacatagaa
    24781 acggtgattt cgtcaccggc tttcaattca gccagttttt cttcagtcaa agcaaactca
    24841 atcaaaccgc gaccggcttc aacacctgct tttgcaaagt gcccggcttc ggctttattg
    24901 acacgattgg cttttttctg accaaaggta acttgaacgg cagtatagcc gtcagtatct
    24961 ttggatttta cttgtgtaac gcggttggca gacatatcca aaacggttac cggaacagaa
    25021 acaccctgtt cgtcgaacac gcgggtcata ccaactttgc gtccaaccag acctaaagtc
    25081 atgattattt tccttttaaa gtaaagggac aggctacgat tggcctgtct ttcagacaaa
    25141 gttaaacttg gcacaaatgt accaagctcg gcattatatc tgacaagtca aagaaatatc
    25201 aacagaaata tcagaaatat cttcaatatg ccgtctgaag cgttttgaat tttcagacgg
    25261 catagggtcg gactctttta aagggtcggt atccggtcat cacagcgtat gcatctttct
    25321 ccgacaaacc ggaatatggt tgatgactgc atccattatt tctttgagat ggtagttttc
    25381 gtatgctggg acctttgcaa aattcctttc cctcccgaca gccgaaaccc caacacaggt
    25441 tttcggctgt tttcgcccca aatacctcct aatt
//
LOCUS       DAWEWP010000011        22889 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWEWP010000011 DAWEWP010000000
VERSION     DAWEWP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.011"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(65..904)
                     /locus_tag="WGN53_000322"
     CDS             complement(65..904)
                     /locus_tag="WGN53_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0139359.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1189..1264
                     /locus_tag="WGN53_000323"
     tRNA            1189..1264
                     /locus_tag="WGN53_000323"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Arg,seq:ccg)
     gene            complement(1329..2522)
                     /locus_tag="WGN53_000324"
     CDS             complement(1329..2522)
                     /locus_tag="WGN53_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0139360.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAATPVPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFESAGFEVGTYPYYDPAAVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2528..3631)
                     /gene="trmA"
                     /locus_tag="WGN53_000325"
     CDS             complement(2528..3631)
                     /gene="trmA"
                     /locus_tag="WGN53_000325"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ0139361.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMLELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     TVERAALFDQFPFTHHIESGVLLKKKILGKSKR"
     gene            3774..4874
                     /gene="aroC"
                     /locus_tag="WGN53_000326"
     CDS             3774..4874
                     /gene="aroC"
                     /locus_tag="WGN53_000326"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ0139362.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRRETDQVEILSGVFKGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4965..5390
                     /locus_tag="WGN53_000327"
     CDS             4965..5390
                     /locus_tag="WGN53_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ0139363.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5464..7449)
                     /gene="parE"
                     /locus_tag="WGN53_000328"
     CDS             complement(5464..7449)
                     /gene="parE"
                     /locus_tag="WGN53_000328"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ0139364.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7516..8040)
                     /locus_tag="WGN53_000329"
     CDS             complement(7516..8040)
                     /locus_tag="WGN53_000329"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ0139365.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLSQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQLSGNR"
     gene            8205..9500
                     /gene="serS"
                     /locus_tag="WGN53_000330"
     CDS             8205..9500
                     /gene="serS"
                     /locus_tag="WGN53_000330"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ0139366.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLEAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPDKSYEALEEMVSHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPTALQPYMGGVAKLEVK"
     gene            complement(9573..10571)
                     /locus_tag="WGN53_000331"
     CDS             complement(9573..10571)
                     /locus_tag="WGN53_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0139367.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRLVLEKLAQIGVKTVALRCAGFNNVDLKTAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGRYVDLDELYARSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSSAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRVDG"
     gene            complement(10696..11772)
                     /gene="prfA"
                     /locus_tag="WGN53_000332"
     CDS             complement(10696..11772)
                     /gene="prfA"
                     /locus_tag="WGN53_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ0139368.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11879..12649)
                     /locus_tag="WGN53_000333"
     CDS             complement(11879..12649)
                     /locus_tag="WGN53_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0139369.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLETMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLEIAQTLRRRVIIHNLKATADIAAAVKRTGFTRGGIVHA
                     FSGSIEEARVLAKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANIPGIAKIAAEIRETCVEEIAKVTERNADALLGR"
     gene            complement(12764..13753)
                     /locus_tag="WGN53_000334"
     CDS             complement(12764..13753)
                     /locus_tag="WGN53_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ0139370.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAELPKYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTHAGEGFIRAVRDFTQQGRLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13820..14503)
                     /locus_tag="WGN53_000335"
     CDS             complement(13820..14503)
                     /locus_tag="WGN53_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0139371.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14692..16026)
                     /gene="glmM"
                     /locus_tag="WGN53_000336"
     CDS             complement(14692..16026)
                     /gene="glmM"
                     /locus_tag="WGN53_000336"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ0139372.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEVELEIEAKIDGEMKTLPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLAQLNQRSWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16156..17007)
                     /gene="folP"
                     /locus_tag="WGN53_000337"
     CDS             complement(16156..17007)
                     /gene="folP"
                     /locus_tag="WGN53_000337"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ0139373.1"
                     /translation="MVGCVWQAGRFEIGLDKPKIMGIVNLTPDSLSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSPEEEWARVESVLAEVAGWGVPVSLDTRH
                     TVVMEKALALGGIDIINDVAALTDEGALELLACQADTGICLMHMQGLPKNMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFCFGKTLQHNITLMRHLPELMAETGY
                     PLLIGVSRKSMIGELTGETDAAARGHGSVAAALAAVARGAKIVRVHDVKATADALKAW
                     EALGINL"
     gene            17282..19396
                     /locus_tag="WGN53_000338"
     CDS             17282..19396
                     /locus_tag="WGN53_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ0139374.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAIGLHASVYRIFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(19461..20939)
                     /gene="ubiD"
                     /locus_tag="WGN53_000339"
     CDS             complement(19461..20939)
                     /gene="ubiD"
                     /locus_tag="WGN53_000339"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ0139375.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(20959..21474)
                     /locus_tag="WGN53_000340"
     CDS             complement(20959..21474)
                     /locus_tag="WGN53_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ0139376.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPYPYNQLSEEERIAYQAECGGDF
                     CIIRYPDQTDRFIRAVLLIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNRLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(21481..21981)
                     /locus_tag="WGN53_000341"
     CDS             complement(21481..21981)
                     /locus_tag="WGN53_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139377.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEK
                     AVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMPFAGEWLYR
                     KLVLKV"
     gene            complement(22213..22782)
                     /locus_tag="WGN53_000342"
                     /pseudo
     CDS             complement(22213..22782)
                     /locus_tag="WGN53_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            22822..>22889
                     /locus_tag="WGN53_000343"
     CDS             22822..>22889
                     /locus_tag="WGN53_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0139378.1"
                     /translation="MLPSKYEDNTLQIKEKSTERTAP"
BASE COUNT         5240 a         6493 c         5975 g         5181 t
ORIGIN      
        1 tcccataaaa aaacggagca gatacctgcc ccgtttttat ttaatccgaa attttaatct
       61 aaatttagaa ttttgcaccg gattggtttg ccatatagtc aacagccgct ttgacttcgt
      121 catcgctcaa accggcattg ccgcctttgg ccggcatcgc gttaaagcct tcaagggcgt
      181 gtttgtgcaa ggtttctttg ccttttttga tacgcggtgc ccaatcgtct tttttgccta
      241 tgccgggaat accgggaatc gaaccgccgt ggcacacctg acaggttgct tcgaagactt
      301 ttttaccgtc aacgccgacc gcaggggctg ccgcaccctt gtcttctgcc ttcgcttctg
      361 ccggagctgc actatcggca ggagcagaag ctgttcctga agcggcattg tcggcaggcg
      421 cagcctcatc aggattcggg aaagaaccgc cgcttttgtt cgccatgtaa gtaatcgccc
      481 gtttgagttc ctgatcggtc aggtctgccg caccgccttt ggcaggcatg gcgttaaagc
      541 cgttcagcgc gtgttggaac aaggtatcga agccttgcgc gatacgcggt gcccaatcgc
      601 cgttgtgttc cagtttcgga gcgttcggca cattgctgtc cgccgcgtgg cattggatac
      661 agattttgcc gaaaatctgt tcgccttggc gttcgccgac ggggatgccg tcgcccatcg
      721 tcaattgtcc gacaggctgg atacgggtct gcgttgctgc ttccgtagtg gcatcgacat
      781 cgccgaacga gccgctgccc gccagcttaa tcaggaaata aaggactgca ataacaataa
      841 cgataccgct cacaagggta aacagtgcag agccttgggc tttgttgtcg cggagttgtt
      901 tcatttggta ggcctcgccg tcaggttagg ttgtgctgta aattatagtt tggtgtgtta
      961 aacgcagtta acaatatttt gctggattat actgaattca cagggtcttt ccaatcgcta
     1021 tcattgaaaa tatgaaaaaa tttgccaacg gtatctgtat aaaacaaata atcctttgaa
     1081 aataatggtt tatcctcaag aaaactctcc ttatgccgcc atacgccgcc tgccggcgca
     1141 agataacctt tgccaatttg cagaatttac gttaacctta cgttttacgc acccatagct
     1201 cagttggaag agtgtcagtt tccgaagctg gaggtcacag gttcgatccc tgttgggtgc
     1261 gccaattata aagagaccgt ctgaaagata aatatttttc agacggtctt ttgacttact
     1321 tcaaactctt atttcaagac ttccgcaaat gcgcgggcaa catagtcggt attcgacgta
     1381 ttcagtccgg cgacgcacat cctgccggaa tccagcaggt aaacggcaaa ttcgtcgcgc
     1441 agcctgcgga cttgttccac gctcaatcct gtgtagccga acatgccgcg ctgtttgatg
     1501 aaataagtga aatcgcgatc ggggatttgc gcagttaaga catcataaag tttctgccgc
     1561 atcgcacgga tgcggtcgcg catcatataa acctcgtttt gccacaaggc gtaaagttcg
     1621 gggctgttca tcacgtcggc ggcgatatac gcgccgtgcg cgggcgggct ggagtagatg
     1681 cggcggacgg tgaatttgag ctgtccgaac accaaatccg cttcttcctt attcgggcaa
     1741 accacgctca ggccgccgac gcgctcgccg tagagcgaca ggttttttga gaaggaattg
     1801 ctgacgaaca agggcaattc catttccacc gctttgcgga cggcgtaggc atcgctgtcc
     1861 aaatcgccgc cgaagccttg gtaggcgatg tccataaacg gaatcagttt gcgcgttttg
     1921 atgatgtgca acacttcgtc ccattgttgt tccgacatat ccacgccggt cgggttgtgg
     1981 cagcagggat ggaggatcag gacgctgttt tcgggcaggg tgttgaaaaa cgcggtcatt
     2041 tcgtcgaatt tcacgccgac ggcagcaggg tcgtaatatg ggtaagtgcc gacctcgaaa
     2101 cctgcgcttt caaaaatgcc gcgatggttg tcccaagtcg ggtcgctgac gtaggcgcgc
     2161 gcttcgggaa accagcggtg cagaaagtcc gccccgactt tgagcgcgcc tgagccgccc
     2221 aaggtttgta cggtaacaat acgtccttgc gcgagcgcgg ggttgtcttt gccgaacaac
     2281 aaatgctgca ccgcgctgcg gtaagtgtct aagccttcca tcggcaggta gggcgacggc
     2341 acaggagtgg cggcgcgggc ggtttcggcg cggctcacgg attccaacac gggcattttg
     2401 ccttcgtcgt cgaaataaat gcctatgctc aaattgactt tttcggggcg cgggtcgttt
     2461 ttgaaggttt cgaccaaact caaaatcgga tcgccgggat agtattcgat gtgtcggtac
     2521 atagtcctta cctcttgctt tttccaagga ttttcttttt caacagcaca ccgctttcaa
     2581 tatggtgcgt aaacggaaat tgatcgaaca gggcggcacg ttcaaccgta tgggtttccg
     2641 ccaaggtgtc caaattggta cgcagcgttt cgggattgca ggaaatgtag atgatgttgt
     2701 caaactgcga caccagcttc aaagtttcct catcgatacc ggcacgcggc ggatcgacga
     2761 aaatagtgga aaatgcgtaa tccgtcaaag caataccgcc atccttaagg cgtttaaact
     2821 cacgttttcc ggtataggct tcggtaaatt cttcagcaga cagacgggcg attttgatgt
     2881 tgccgatgcg gttggcttcg atattccatt gcgccgcgct gacggaggtt ttggagattt
     2941 cggttgccaa aacacgttca aacttttcag ataaaggaag cgtaaagttg ccgtttccgc
     3001 aatacagttc gagcaaatcc ccatccaatc cttctgctgc accgcaggcc cattcaagca
     3061 ttttctgaca cacggcggca ttcggttggg taaaactgcc ttcaatttgc cgataacgga
     3121 aatcccggtt gccgaccttc aaagtttccg ttacatagtc ctgttttaag actattttct
     3181 gtcccctgct ccgcccgatg acggaaatat ccaactgttg ctgtaacgct tgcgccgcct
     3241 gcatccactc attatcaagc cttttgtggt aaatcatggt aaccagcatt tccccgctga
     3301 gcgtagacaa aaattcgacg gcataccaac ggtttctcag ttcggcagat tgggcggcaa
     3361 cggcaatcaa ttctagcatc aggcggttga cggcttcgga agccgcttca aaacggtcgc
     3421 aacggatcag gcttgccccg ccagcctttt gtcctttttc aaacatggca taaaacattt
     3481 caccgccttc gtgccaaata cggaactcgg cacgcatacg gtaatgtttg tccggagatt
     3541 cgtacacttc ccactcagga acatccaaac ctgcaaaaag ggttttaagg tggtcttttt
     3601 tatcttgaag ctgctgcgtg tagtcgctca tatcgctatc ctgaaaaatc agaccggatt
     3661 ataagccgat tcactgcaaa acacgcaacc cctttaccgg catataaccc gttttcctcc
     3721 aacttttatg ctaaaatccg ccgtctccaa cataccgccg acagaaggaa aatatggcag
     3781 gcaacacttt cggacaactc ttcaccgtga ccacgttcgg cgaaagccac ggcgcaggtt
     3841 tgggctgcat catcgacggc tgcccgcccg gcttggaatt aagcgaagcg gatatccaat
     3901 ttgacctcga ccgacgcaaa cccggcacca gccgccacgt tacccaacgc cgcgaaaccg
     3961 accaagtaga aatcctctcc ggcgtattca aaggcaaaac caccggcacg cccatcgccc
     4021 tcttaatccg caataccgac cagcgcagca aagactacgg caacatcgcc caaagtttcc
     4081 gccccggcca tgccgactat acctattggc acaaatacgg cacgcgcgac taccgcggcg
     4141 gcggcaggag ctccgcccgt gaaaccgccg cccgcgttgc tgccggagcc gttgccaaaa
     4201 aatggttgaa agaaaaattc ggcacggaaa tcaccgccta cgttacccaa gtcggcgaaa
     4261 aagaaatccg gtttgaaggc tgcgaacaca tttcccaaaa tccttttttt gccgccaacc
     4321 atagccaaat tgccgagctg gaaaactata tggacagcgt gcgcaaatcg ttggattccg
     4381 tcggcgcgaa gctgcatatt gaagccgcca atgtccccgt cggcttgggc gaacccgttt
     4441 tcgaccgcct cgatgccgaa atcgcctacg cgatgatggg catcaacgcc gtaaaaggcg
     4501 tggaaatcgg cgcaggtttt gacagcgtaa cgcaacgcgg cagcgaacac ggcgacgaac
     4561 tgaccccgca aggcttcctg tccaaccatt cgggcggcat cctcggcggc atcagtaccg
     4621 ggcaagacat ctgcgtcaat atcgccatca aacccaccag ctccatcgcc accccgcgcc
     4681 gcagtatcga catcaacggc aaccccatcg aactcgccac gcacggcagg cacgacccct
     4741 gcgtcggctt gcgcgccgcg ccgattgccg aagctatgct cgccttggtt ttaatcgacc
     4801 acgccctgcg ccatcgtgcg caaaatgcgg atgttgccgt ggatacgccc gatatcgccc
     4861 gctcggacaa gtaaaaagat ttagccaaaa cacgggcttt atactaaaat accgaccatt
     4921 gccgtgccgt ctgaacgcac gattcttaga ggaatacaac cgaaatgaca caagaaaccg
     4981 ctttgggtgc ggcactgaaa tccgccgtcc aaactatgag caaaaagaaa cagaccgaaa
     5041 tgatcgccga ccacatctac ggcaaatacg atgtgttcaa acgcttcaaa ccgttggcac
     5101 tcggcatcga tcaggatttg attgccgctt tgccgcaata cgatgccgca ctgattgcac
     5161 gcgtcctcgc caaccactgc cgccgtccgc gctatctgaa agccttggcg cgcggcggca
     5221 aacgtttcga tttgaacaac cgtttcaaag gcgaagttac ccccgaagaa caggcgatcg
     5281 cgcaaaacca tcctttcgtg cagcaggctt tgcaacagca gtccgcccaa gctgccgccg
     5341 aaacgccgtc tgttgaagcc gaagcagccg aatcttccgc agcagaataa atccccaaat
     5401 gaaatgccgt ctgaaaaccg atttggtttc agacggcatt tttccgtatg cggcaatcac
     5461 ggttcaaata tcgagttccg ccgtatcgcc ttcgcgctcc atccaggcac ggcgggcggc
     5521 ggcttcgcct ttgcccatca gtttgacgaa gatgtcgcgc gtctcgtcat ccgcgccttc
     5581 ggggatttgt acctgcaaca ggcggcgggt gtcggggtgc atggtagtat ctttgagctg
     5641 gtctgggttc atctcaccca aacctttgaa acggctgatg gaataagcag tttctttaac
     5701 gccttctttt tgcagccgct ctaaaatgcc gtcaagttcg ttttggtcga gggcgtagaa
     5761 tttgcgggcg ggtttgcttt taccttgtgc gttgacatct acgcggaaca gcggcggctg
     5821 ggcgacgtag atgtgtccgt cagcgaccag tttcgggaag tggcggtaga acagggtcag
     5881 cagcaaaact tgaatatgcg agccgtccac gtcggcatcg gacaggatgg cgattttgcc
     5941 gtaacgcagg ccgcttaaat cgggatggtc gttgatgccg tgcggatcga cgccgatggc
     6001 aactgaaatg tcgtggattt cggcgttgcc gaagagctgg tcggggtgga cttcaaagct
     6061 gttgagcact ttgccgcgca ggggcaggat ggcttgggtg gctttgtcgc gggcgagttt
     6121 ggcggagccg ccggcggaat cgccttcgac gaggaagagt tcgttttcgc ggatgtcttc
     6181 gctttcgcag tcggtcagct taccgggcag gacggcgacg ccgctgcctt ttttcttttc
     6241 aatcttttta accgaacgca tccgcgcctg tgcctgacgg atggcgagtt cggcgatttt
     6301 tttgccgaag tccacgtttt ggttcagcca caattccaaa gggtcgcccg atacggtagc
     6361 aacgagtttc agcgcgtcgc ggttggtcag tttgtctttg gtctgacctt ggaactgcgg
     6421 gtcgaggaca cgggcagaga ggacaaaggc ggttttgccg aacacgtcgt cgctttgcac
     6481 tttcacgccg cgtggtaaaa ggttgtgcag attgatgaag ttgctgacgg cgttgaacac
     6541 ggcttgtttc aagcccgctt cgtgcgtgcc gcccagcggg gtggggatga ggttgacgta
     6601 gctttcgttg gcgcacgagc cttcttccag ccaggtcagg gcaaacgcgg cgccttcgcc
     6661 gatgctgaaa tcgccgttgt ggccgtctga aatgtagttt tcgcaagaga agataggcac
     6721 ggcttcctgc gcgtcggcaa tcaggtcggt cagatagctt ttcagaccgt cggggtaatg
     6781 ccaggtttgg gtgtgcgctt cgtcttcgcc tttgaccgga cgggtcaggg aaacgcgcac
     6841 gcccggcagc agcacggctt tggcgcgcag caggcgttcg agttcgggga tgctgtaatt
     6901 cgggctttca aaatatttgc cgtccggcca gacgcgcact tcggtgccgc tgtctttgac
     6961 ggcgcatttg cccacttgtg ccaacggttc gaccacgtcg ccgccggcaa acacgatgcg
     7021 gtggattttg ccttcgcgtt tgaccgttac ctcaaggcgg gtggaaaggg cgttggtcac
     7081 ggatacgccc acgccgtgca ggccgcctga aaaggcatac gcgctgccgc cgtctttttt
     7141 gttgaacttg ccgcccgcgt gcagacgggt gaacacgagt tcgactacgg gtacgccttc
     7201 ttcagggtgc agaccgacgg gaataccgcg gccgttgtcg cgtacggaaa gcgaaccgtc
     7261 ttcatgaatt tgcacgtcga ttgccgtagc gaaaccgccc aacgcctcgt ccgccgcgtt
     7321 gtcgatgact tcttggcaga tatgggtcgg gctgtcggtg cgggtgtaca taccgggacg
     7381 ttctttgacc ggttccaaac ctttgaggac ggtgatgctg gattcgctgt attggttgtt
     7441 tttagccata ggaataatct gaaagtaaga aaaacaacgc tttcagacgg cctgaaagcg
     7501 ttgcgttccg ttgttttacc ggttgccgga aagttggcgg gcggcaaagt cttcataact
     7561 ttccataccg cgcaggaagc gggaagaaag ttctttcaat gcccccaaat aaacgtcgcg
     7621 tttgaaatca atcacttcgt caacgggcgc ccaatattgg tgccaacgcc agccgtcaaa
     7681 ttcggggtgg cgggtggcgc gcaggttgac atcgcaatcc cggccggtca ggcgcaggag
     7741 ataccaaatc tgcttctgcc cgcgataaga gccgcgccat tcgcggcgta cccagttgtt
     7801 cggcacgtcg taacgcagcc agtcgcgcgt ccgcccgacg attttgacgt gttgcgacag
     7861 aagtccgact tcttcgtaaa gctcgcggta catcgcggtt tcggggcttt cgcccggctt
     7921 gatgccgcct tgcggaaact gccaagaatg ttcgcgcacg cgcttacccc aaaagacttc
     7981 gtcgcggttg ttggttaaga tgataccgac attggggcga tagccttccc tgtccaacac
     8041 ggtgtcgccc tccgttaaat tcaatcttgg gattttccca caaatcgaac ggttttgaca
     8101 aatcagacgg tatggttcaa gcatgtcgaa acacagacgg actcgggaaa atatcttaaa
     8161 tttggtttac aataatgtgt ttcaaattat tcgggaatca gaccatgtta gacatccaat
     8221 tgctccgcag caacaccgcc gccgttgccg aacggcttgc acggcgcggt tatgactttg
     8281 ataccgcacg ttttgacgca ctggaagaac gacgcaagtc cgttcaggtg aaaaccgaag
     8341 aattacaagc ctcgcgtaac agcatttcca agcaaatcgg cgcactgaaa ggtcagggca
     8401 aacacgaaga ggcacaggcg gccatggatc aagtcgccca aatcaaaacc gatttggaac
     8461 aggcagccgc cgatttggaa gccgtgcaaa aggaactgga cgcatggctg ttgagcattc
     8521 cgaacctgcc gcatgaggac gtgcctttcg gtaaggatga aaccgaaaac gtcgaagtcc
     8581 gcaaagtcgg caccccgcgc gaatttgact ttgaaatcaa agaccatgtc gatttgggcg
     8641 aacctttggg tttggatttc gaaggtggcg cgaaactttc cggcgcacga tttaccgtga
     8701 tgcgcggaca aatcgcccgt ctgcaccgcg ccttggcaca gttcatgctg gatacgcaca
     8761 cgctgcaaca cggctacacc gagcattaca cgccttatat cgttgacgat acgacgctgc
     8821 aaggtacggg ccaactacca aaatttgcgg aagatctgtt ccacgttacc cgtggcggcg
     8881 acgaaaccaa aaccacccaa tacctgattc cgacagccga agttaccctg accaataccg
     8941 ttgccgacag cattatcccg tccgaacaac tgccgctgaa gctgaccgcg cattcgccct
     9001 gtttccgcag cgaggcggga tcatacggca aagacacgcg cggcctgatc cgccagcacc
     9061 agttcgacaa agtggaaatg gttcaaatcg ttcatcccga caaatcatac gaagcactgg
     9121 aagaaatggt cagccatgct gaaaatattt taaaagcgtt ggaactgcca taccgcgtga
     9181 tcactttgtg taccggcgat atgggctttg gcgcggcaaa aacgtatgac ttggaagttt
     9241 gggtgccggc gcaaaatacc taccgcgaaa tctcaagctg ctccaactgc gaagatttcc
     9301 aagcccgccg catgaaggcg cgtttcaaag acgaaaacgg caaaaaccgc ttggtacata
     9361 ccttgaacgg ttccggcttg gcggtcggca gaacgctggt cgccgtattg gaaaaccatc
     9421 aaaacgccga cggcagtatc aacatcccca ccgcactgca accgtatatg ggcggtgttg
     9481 ccaagttgga agtgaaataa gccttaaccc tgaacataaa tgccgtctga aacctgtttc
     9541 agacggcatt tcctttaaac ttttaaaaca cgtcaaccgt cgacacgaac cgcattgccg
     9601 caatcgccgg tctgtccgac ctcgcggata ttggacaacg taacttccga aatattacct
     9661 aacgcctctt ccgtcaaaaa cgcctgatgt ccggtaaaca gcacgttatg gcaggacgac
     9721 agtcggcgga acacgtcgtc ggtaatcaca tcgttggatt tgtcttcaaa aaacaattcg
     9781 cgttcgtttt catacacgtc catacccaat gcgccgattt tccggcgttt caacgcctca
     9841 atcgcggcag aactgtcaat cagaccgcca cggctggtgt tgataatcat cacaccgtct
     9901 ttcattttgt cgaacgccgc ttcgttcagc atatagtggt tttccggcgt ggaggggcaa
     9961 tgcagcgtga tgatgtccga tcgggcatac agctcgtcta aatccacata cctgccgccg
    10021 agtttttcca cttcggggtt gcaaaacgga tcgtaagcca gcaggttcat gccgaaaccc
    10081 tttaaaatcc gcatggttgc aataccgatt ttccccgtgc cgataacgcc cgccgttttg
    10141 ccgtacatat tgaaaccggt cagaccttcc agcgaaaaat tcgcatcgcg agtacgctga
    10201 taggctttgt ggatacggcg gttcaaagtc agcatcagac cgaccgtatg ttccgcaacc
    10261 gactcgggtg aataggcggg cacgcgcacg actttcaagc ccaactcttc agccgtcttc
    10321 aaatccacat tattgaagcc ggcacaacgc aacgccacag ttttcacgcc aatttgcgcc
    10381 aatttttcca acacgagccg gctgccgtcg tcgttcacaa aaatacagac cgcttccgcg
    10441 ccttccgcca ttttcgccgt tttcgcatcc agcatgaaat caaaaaactc cagctcgaag
    10501 ccgaaatgcc ggttggcgcg ggtaaaatgt tcgcggtcat agcttttcgt accgtaaatc
    10561 gcaatcttca tcaatatgtc cagttgtcgt ctatggttga gaaacggcat aatacactga
    10621 attcaaacaa atcagtagaa tatggcagat taaaattgat tgcatgcacg gcatttccat
    10681 ttcaaaacac aaaactcaat cgcccattgc cgccagaagc tccgcctgat gctcggcaat
    10741 cagggcatca gtgatttctg ccaaatcgcc gtccatgaca aaatccagct tgtgcagggt
    10801 aaggttgatg cggtggtctg ttaccctgcc ttggggatag ttgtaggtgc ggatgcgctc
    10861 gctgcggtcg ccgctgccga tgagggattt gcgctcggcg gcttctttgg cttgggcttc
    10921 gcgtttttgc gcgtcgttca ggcgggcggc gaggactttc attgcctgcg ctttgttggc
    10981 gtgttggctg cgtccatctt ggcattcgac caccatgcct gtgggcaggt gggtgatgcg
    11041 gacggcggaa tcggttttgt tgatgtgctg accgcctgcg ccggatgcgc ggaaggtgtc
    11101 gatgcgcagg tcggcggggt tcaactcgat gtcttcgagt tcgtccgctt cgggcatgac
    11161 ggcaacggtg caggcggagg tgtggatgcg gccttggctt tcggtggcgg ggacgcgctg
    11221 cacgcggtgt ccgcccgatt caaatttcaa acggctgtac gccccgagtc cgacaatacg
    11281 ggcgatgact tctttatagc cgcccaactc gctttcgttg gcggacacga tttcgacctg
    11341 ccagcggttg cgttcggcgt agcggctgta catacgcagc aaatcgccgg caaacagcgc
    11401 ggcttcgtcg ccgcccgttc cggcgcgtat ttcgatgaag atgtttttgt cgtcgtcggc
    11461 atctttgggc agcagcagtt tttgcaattc ggtatcgagt gcgccgattt tggctttcgc
    11521 cgcttcgatt tcttcggcgg caaagtcttt catttcgggg tcggacaaca tttcttcggc
    11581 atccgccaaa tcgctttggg caagccgata gttttggaat acttcgacga cgggggtcag
    11641 ttcggcgtgt tcgcgcgtga gcttgcggta gttgtccata tcggacgtgg cttcgggttg
    11701 ccctaaaagg tgggtaactt cttccaaccg gtcgccgagt tgttgcagtt tttctaagat
    11761 agacggcttc ataattcttc cataacaaac gccgcctgaa tttcagacgg catcaacact
    11821 gaattattat tataataggt tttccggaca ttcaaaaaga taatctcaga tggataacct
    11881 accgtcccaa cagggcatcg gcattgcgct ccgttacctt ggcaatctct tccacacagg
    11941 tttcgcggat ttccgcagca atctttgcaa tacccggaat attggcaggc gtattaatct
    12001 cttttttcag cataaacggg ctgtcggttt ccaatacgaa atccccgtcg ttcaaggctt
    12061 taagcgtatc gcgcacttta cgcgcgttcg gattgagcag cagcgaaccg atgccgattt
    12121 tgaaacccag tttcgccaac acgcgcgctt cttcaatact gccggaaaag gcgtggacaa
    12181 tgccgcctcg ggtaaagccc gtccgtttga cggcggcggc gatgtcggcg gtggctttca
    12241 gattatggat aatcacgcgg cggcgcaggg tttgcgcgat ttcaagctgg cggacgaaaa
    12301 cttggatttg ccgttcgcgc tgcggcggcg tttgggtttt gtcgtaaaaa tccaagccga
    12361 tttcgccgac ccatgcctgc ggataacgtg ccaacatcgt ttccaggcgg acgcaatcac
    12421 gctcggcaat gccgtctgaa aaccaaggat ggatgccgag cgcaatacgg atttgaccgt
    12481 gttcggacgg catttccgac agttccgcca cgctttgcca atcctgcgga caggtggcgg
    12541 gaacgataaa ccgcttcacc ccaactttcc gcgcggcggt caggatgtgc ggcaggtttt
    12601 cgcgcagggc ggcatcggca aggtggcagt gggtgtcggt aaagttcatt tcgattccgc
    12661 aatgcggcag gttcacataa aaataaggca gggcggcaag ggatgcagtg tacgggcggc
    12721 gcataaggcg cggtcaacgc cccgtcatta ccatttcaat ccgctataac ttcagtcttg
    12781 ccaactcttt ttccacctct tccttacccc agccttgcga tacggcgagg gtcatgagtg
    12841 cggtggcggt ttcgaggttg catttgccgc cgttgatgat gcccgagttg cggaacgcat
    12901 tgccttgcgc gtaaacggcg gcggttttgc cttgtcggac ttggctgatg ttgagcagca
    12961 gcctgccttg ctgcgtgaag tctcggacag cgcggatgaa gccttcgccg gcgtgggtgt
    13021 tgccgtgtcc gtaactttgc aggataaggg cgtgggcggg aagctgtccg agtccgtcgg
    13081 caagttcttg gacggcaaag ccgggaataa gcgtgcggac ggcgattttt gcctgcgggt
    13141 caggatagcg gattttgagg ccgtctgaaa catctgccgc gtcctgattc gggatgcgga
    13201 cattgtgcca gccctgggtt tcgtcccact ccgccagtgc gccgaaatgc ggattgtcga
    13261 aaccggcggc ggtttcggtg ctgactttgc tgctgccgac ggcgggatac agtttgccgt
    13321 caaaggcaat aagcgtctgc ttcaatccga gtgggaatgc ggcaacggca gtcgccagat
    13381 tgcgcggcgc gtcgctgccc acggcgttgt acggccattg ggaaccggtc aggacgatcg
    13441 gtttgtccaa accttgcagg gcgagggcga acagattggc ggtataggcg agcgtgtccg
    13501 tgccgtgcag caccaaaacg ccgtcgtatt tcggcagctc ggcggcaatg atgtccagcc
    13561 aagcgcacca gttttgcagt tggacggcgg aagaatcaat cagcgtccca caaacgtgcc
    13621 aatcgaaatc catgccgttt gaaaacggcg cgagtgcctg cccgaccagc gcggtatcgg
    13681 ggcgcaggcc ttcgctgctt tgcgtcatgc cgatggtgcc gccggtataa aggacaaaaa
    13741 ttttctgttt catggctcat cctgaaaatg caaagaccgc ctgaaacgcg aaatgcgtcc
    13801 agacggtcga aaatctgatt tactgctgct tctgtgccgc ttttttcgct gccttttccg
    13861 ccttgcgttt ggcgcgcttt tcgctcaatt gtgcgcggta aagctgataa tgtcggcgtt
    13921 tgcgccacca gaaccacgcc agcgccgccg ccagcacgcc caatgcgatg aagatgccgg
    13981 attgcaggct gtgcattttc gccatcagcc aatcgatgtt gtgcgcgccg tactcgccca
    14041 agtaaatcca aacgggcacg gaaatcagcg cggcaagccc gtccataatc agaaagcgca
    14101 gatacgatac tttgcggctg atgccggcgg taacgaaaac ggcagtccgc aaacccggca
    14161 ggaaacgagc gacaaacaga acccagttgc cgtatttgtc gaatttttcc tgaacctgtg
    14221 cgtaacgttt cggcgtcatg atgcgcgcaa tcggtttgaa cttgaggatt ttctgccccc
    14281 agatgcgtcc ggcggcgaac atgatgccgt ccccgaccaa tacgccgagc ataccgactg
    14341 caaacataat atgcggattg gtataaccca tacccgaaat cacgccgcct gttaccaagg
    14401 tcaaatcctc gggaatcggc acgccgaaac cgcagatgac caaaacgaaa aacacggccg
    14461 catagccgta ttcgacaaaa aaggcttcca aaagggcaaa catagcggat attccattgt
    14521 cggagataaa aagtcagaac aagccgaaac attttctgcg cgaaacggca ttctatcaaa
    14581 gattatgccg tctgaaagcg gcaaggggca gattcgcgcc ctctgccgga cgttcagacg
    14641 gcatttaaac aggaaaaccg ccgctgttga aacggttttg cctgattttg cctaaaggct
    14701 gccgatggcg gaagcaatac gttccgcacc gtcgcgcgcc caatccgcct gccgcgcctc
    14761 caccatcaca cgcacgacgg gttcggttcc cgaagcgcgc aacacgacac gccctttgcc
    14821 ttcgagttct ttttccactt ccgccaacac gtcttttgag gcttcctgcc atttctgacc
    14881 tttttggatg cgcacgttaa tcatcgtttg cggatacggc tgccaatcgg cgcaaacggt
    14941 ggcgaggtct tggttcagcg tttgcagtgc cgccaaaact tgcagcgcgg aaataatgcc
    15001 gtcgccggtg ttgtgtttgt ccatgcacaa aatatggccg ctggcttcgc cgccgatgag
    15061 ccagctgcgt tggttcagct gtgccaacac atagcggtcg ccgactttgg cgcggcagaa
    15121 atccacgccc tgctctttca gggcgatttc catcgccata ttggtcatga ccgtgccgac
    15181 cacgccgccg atgttgatac cttctcgggc gcgggcttta gcaatgacgt aaatcaggct
    15241 gtcgccgtcg taaaccttac cgtttttatc gaccatcatc aggcggtctc cgtcgccgtc
    15301 taaggcgatg ccgtagtcgg cttcatgctg taaaacggct gcctggagtg tcttggtata
    15361 agtcgcaccg catttttcat tgatattgta gccgttgggt tcgtcgccga tgctgacgac
    15421 ttgtgcgccc agttcgtgaa acaccttggg tgcgacaccg taccccgcgc cgttggcagt
    15481 atcgataacc aacttcaaac cccgaaggtc ggaatggctg ggaaaggtgg atttgcaaaa
    15541 ttcgatatag cggtcgtccg caccgctgat gcggcgtgcg cgaccgaggc gggcggacgg
    15601 gagggttttc atttcgccgt cgattttggc ttcgatttcc aactcgactt catcggaaag
    15661 tttcacgccg ccttcggcga agaatttgat gccgttgtcg gaataggcat tgtgcgacgc
    15721 ggaaatcatc acgccggcgg acaggcgcaa cgcgcgggtc agataagcca cgccgggcgt
    15781 gggcagcggt ccggtctgta ccacattcac acccgccgcc gtaaaacctg ccaccaaagc
    15841 ggcttccaac atataaccgg aaatacgcgt atccttgccg attaaaacgg tcggtttctg
    15901 gtcggtgtcg tgctgaacca aaacctgacc cgccgcatag ccgagtttca atacaaaatc
    15961 gggcgtaatc ggaaactgcc cgacttcgcc gcgcacgccg tccgtgccga aatatttttt
    16021 tgccatgtgt tgctccgaga atgtgaaccg ttgtccgaga ttatacagtc tgtttgtacc
    16081 ttgctgtctg caccgttgat gccgtctgaa gccgctccgc ccttttcaga cggcatgaag
    16141 tatgtgaacc gctgtttaca gattgatgcc caacgcttcc cacgccttca aagcatccgc
    16201 cgtcgccttc acatcgtgca cgcgcacgat tttcgcgccg cgtgccacag ccgccaaagc
    16261 cgccgccacg ctgccgtgtc cgcgcgccgc cgcgtcggtt tcgcccgtca gttcgcctat
    16321 catgcttttg cgcgacacgc cgatcagcag cggatagccc gtttccgcca tcaattcggg
    16381 cagatgccgc atcagtgtga tattgtgttg caaggttttg ccgaagcaga aaccggggtc
    16441 gagtgtaatg cgttgcgggg cgatgcctgc cgcgatgcat tccgccgccc gcgctttcaa
    16501 ataccgcgcc acttcaccga caacatcctg atatttcgga ttaatctgca tattcttagg
    16561 caagccctgc atatgcatca ggcaaatgcc cgtatccgcc tgacacgcca gcaattcgag
    16621 cgcgccttcg tcggtcaacg ccgccacatc gttaataata tcgatgccgc cgagtgccaa
    16681 cgctttttcc ataaccaccg tgtggcgcgt gtccaaactg acgggaacgc cccaccctgc
    16741 cacttccgcc aatacagact caacccgcgc ccattcttct tcaggcgaaa cataatccgc
    16801 acccggccgc gtcgattcgc cgccgatgtc gagaatgtcc gcgccctctt tcaaaagccg
    16861 ttcggcatgc gccaaagctg tttgggcgtt ttgcgaatac gcgccgccgt cggacaaaga
    16921 atcgggcgtg agattcacga tacccatgat tttcggtttg tccaaaccga tttcaaaccg
    16981 tcctgcctgc caaacgcatc cgaccatatc cgtgcctccg gaataaaaag caaattatat
    17041 accgtctgaa caaaacttgt ccgtttcaga cggcatcagt atttcaaaaa agtatcttgc
    17101 agaatccgcc gccgtatgaa acatacactg cccctgcaaa cgcggcaacg cccacgatac
    17161 ctgatccggc ggtatgcctg cttatgggca ttaccccttt atacaagata atccgcccac
    17221 acaaaatagg atttctacaa gccgtgttat actgtggcgt gttttacaga cttttcaggc
    17281 tatggattta ttatcggtct tccacaaata ccgtctgaaa tatgcggtag ccgtgctgac
    17341 gatactgctt ttggcggcaa tcgggctgca cgcttccgta tatcgcatct tcacacctga
    17401 aaacatccga agccgcctcc aacaaagcat tgcccatacg caccggaaaa tctcgtttga
    17461 tgcggatata cagcgcaggc ttctgccccg gccgaccgtc atcctgaaaa acctgaccat
    17521 taccgaaccc ggcggcgacc ggactgccgt ttccgtccaa gaaaccaaaa tcggattgag
    17581 ctggaaaaac ctgtggtcgg atcagataca gattgaaaaa tgggtggttt cgagtgcgga
    17641 acttgccctg acgcgcgacg ggaaaggtgt ttggaacatc caagacctga tcgacagcca
    17701 aaaacgccaa gcctcagtca accgcattat cgtcgaaaac agcaccgtcc gcctcaattt
    17761 cctgcaggaa cagcttatcc tgaaggaaat caacctcaac ctgcaatccc ccgattcgtc
    17821 ggggcagccg tttgaaagtt cgggcatact ggtttgggga aagctgtccg tcccgtggaa
    17881 aagcaggggg ctgttccttt cagacggcat cggcacgccc aaaatctcac cgttccattt
    17941 tgaagcttcc acttcgctgg acggacacgg cattaccatt tccaccaccg gcagcccttc
    18001 tgtccgcttc aacgccggcg gagcggatgc cgccggcctc ggcctgcgtg cagacacttc
    18061 cttccgcaac ctccacctga ccgcccaaat ccctacgctg gcactcagga acaacagcat
    18121 taaaattgaa accgtcaacg gcgcatttac cgccggcggc gaatatgccc aatgggacgg
    18181 ttcgttcaaa ctcgacaaag ccaacctgca ctccggcatc gccaacatcg gcaacgccga
    18241 aatctccggc agcttcaaaa caccgcgcca ccagaccaac ttctccctca attcgccgct
    18301 cgtatggacg gaaaacaaag ggctggacgc gccgcgcctg tatgtatcga cccttcagga
    18361 taccgtcaac cgcctgccgc aaccccgttt catcagccgg ctcgacggtt cgctgtccgt
    18421 accgaatctg caaaattgga atgccgaatt aaacggcaca ttcgaccgcc aaaccgttgc
    18481 cgcgaaattc agatacacac atgaagacgc accgcatctg gaagccgccg tcgcactgca
    18541 aaaattgaac ctgaccccct atcttgacga cgtgcggcaa caaaacggca aaatatttcc
    18601 cgacaccctc gccaagctgt ccggcgacat cgaggcgcac ctgaaaatcg gaaaagtcca
    18661 acttcccggc ctgcaactgg acgatatgga aacctacctc cacgccgaca aaggccatat
    18721 cgcgctcagc cgtttcaagt cagggcttta cggcggccat accgaaggcg gcatcagcat
    18781 cgccaacacc cgtcccgcca cttaccgcct gcaacagaat gcaagcaaca tccaaatcca
    18841 accgctgctg caagacctgt tcggcttcca cagcttcagc ggcaacggcg acgcggtcat
    18901 cgacctgacc gcgggcggcg aaacccgaaa agagcttatc cgctcgcttc aaggcagcct
    18961 gtcgctgaat atttccaatg gcgcatggca cggtatcgac atggacaata tcctgaaaaa
    19021 cggcatttcg ggcaaaactg ccgacaatgc cgcacccagc acacccttcc accgattcac
    19081 gctcaacagc gaaatttcag acggcatcag ccgccacatc gataccgaac tcttctccga
    19141 cagcctctat gttaccagca acggctatac caatctggat acgcaggaat tgtctgaaga
    19201 tgtccttatc cgcaacgccg tccatccgaa aaacaaaccg attcccctga aaatcaccgg
    19261 tacggtggac aagccgtcca ttaccgtcga ttacggcagg ctgaccggcg gcatcaattc
    19321 gcgcaaagag aaacagaaaa tcctcgaaga caccctgctg gaacaatggc agtggctcaa
    19381 acctaaagaa ccgtaaacat cctgcgtaca aaaatgccgt ctgaaacacc cccgcgcttc
    19441 agacggcatg accgtaaaac ctacaacccc aattcctccc aaatcccatc aatcttagcc
    19501 gtaaccgcag ggtctttttt gatgacgcgt ccccattcgc ggtcggtttc gcccggccat
    19561 ttgttggtcg catccaaacc cattttgccg ccgagtccgc tgacggggct ggcgaagtcg
    19621 agataatcga tgggcgtgtt ttctaccaaa acagtgtcgc gcacggggtc catgcgcgtg
    19681 gtgaccgccc agatgacttc tttccagtcg cgtacgttca catcgtcatc caccacgatg
    19741 atgaatttgg tgtacataaa ctggcgcagg aacgaccagc agcccatcat cacgcgcttg
    19801 gcgtgtccgg cgtactgttt tttcatgctc accaccgcca tgcggtagga gcagccttcg
    19861 ggcggcaggt aaaaatcggt gatttcgggg aactgctttt gcaagagcgg tacgaacact
    19921 tcgttcaatg ccacgcccaa aacagcgggt tcatcgggag gtttgcctgt gtaggtcgaa
    19981 tggtaaatcg ggttttcgcg catggtgatg cgctcgaccg taaatacagg gaaatgatcc
    20041 tgctcgttgt aatagccggt gtggtcgccg tatgggcctt ccaacgcggt ttcgttcgga
    20101 tgaatcacgc cttccaatac aatttcagca cgggcaggca cttgcaaatc gttgccgata
    20161 catttcacca gctccgtccg cgaaccgcgc agcagtccgg caaactggta ttcgctcaaa
    20221 gtatcgggaa caggcgttac cgctccgaga atagtggcag ggtcgcaacc gagcacgacg
    20281 gcgacgggat acggcgtatc gggattgagt ttgcggaact cctgataatc caacgcgccg
    20341 ccgcgatgcg acagccagcg cataatcagc ttgtttttgc cgatgagttg ttggcggtaa
    20401 atgccgagat tttggcgttt tttgtgcggc ccgcgcgtta cggtcaagcc ccatgttacc
    20461 agcggcgcaa cgtcttccgg ccagcaatgc tgaatcggaa gtttatacaa atcaacgttt
    20521 tcgccttccc acacgatttc ctgacacggc gcgtttttca ccacgttcgg cgccatgctc
    20581 caaatgtctt tcagtagcgg cagtttggaa aacgcatctt tgatgccttt gggcggttcg
    20641 ggttctttca aatacgccag cgtctgcccg atttcacgca gcttggacac gctgtccgcg
    20701 cccatgccca tcgccacacg ttcgggcgtg ccgaacaggt ttgccaacac aggataaccg
    20761 tagcgcgtac cgtcgggctt aaccgggttt tcaaacagca acgccggccc ttcggcgcgc
    20821 agcacgcggt cggcgatttc ggtcatttcc aaatgcgggg aaacggggtg cgcgatgcgt
    20881 ttgagtttgc cctgctgctc gagcatggcg atgaagtcgc gcaggtcttt gtatttcata
    20941 ttcatccttt ttgtcctttt atcctgagca atccgattcg gataccgccc ctatccttgc
    21001 ctgcgcttcg gcatattcta tgccgtgata aaagtcgcgt accagcggat gttcgctgcc
    21061 ttgatggagt tgcaacaaag gacgttgacc atcgggttgg gtaacgacat tgcaatgcaa
    21121 accgaaggtg tcggtttcat aagggggcag ccggttgcag atcatcccga aataaacgac
    21181 attttccgga ttgtcgtgaa agtggctttg atagtcgtta aaactctttt cgctgacgga
    21241 cacccacacg ccgtattcca gcgtttcctc atgcccgata atcggaataa gcagcaccgc
    21301 gcggataaaa cggtcggttt ggtcgggata gcggatgatg cagaaatcgc cgccgcattc
    21361 agcttggtaa gcaatgcgtt cttcttcact gagttgatta tagggatagg gggcggtaaa
    21421 gccgattgcg ggcatatctt catgtttttc gccgcaggaa gtgcaagtgt acataaggtt
    21481 tcagactttc aaaacgagtt tgcggtaaag ccattcgccg gcaaacggca tccccatcaa
    21541 tacataggca atcacgccgg tataaaccgc ccaccaatca tatcgcccca accgtgccaa
    21601 caaagcggca agcgtcccgt tggcaataaa gaatacgcac caaacctgcg ttacccggcg
    21661 ggtatagcgc acagcttttt caggcaggtc gggctgttgc agccgcgcaa gtttttcaat
    21721 caccgtctgc ccggcaaaca ggctgccgcc gaacaccgcc aacatcatca gattgacgag
    21781 gacgggatac caatacatcg aatcatgccg cccgaacacc aatactgcgg caaaaaacag
    21841 tgtaataaac aaagccgcat aacgctgttg gggcagtttg gcagtcaggg cgcgcagcag
    21901 ccacaacccg cacatcgccg ccgccagcca gacaaaccag cccgcctccc tgccgtaata
    21961 tagtggatta acaaaaacca gtacagcgtt gcctcgcctt gccgtactgt ctgcggcttc
    22021 gtcgccttgt cctgattttt gttaatccac tataccacaa agcgggatag gcaatgctta
    22081 atacggtcag aaaaatatgt ccgaaaaaac cgggtttcat tttgaatccg cacaaatgtt
    22141 ttcagacggc atccgataaa aacatgccgt ctgaaaaata attagaaata cccgattagc
    22201 ccgcctgaat cttcaatacc gcctgtacca cgtcgttgac ggtgcggaca ttgcggaaat
    22261 cttcagcctg cagcttgcgg gctgccacag ccattcacga ttgcgatata cccggcggca
    22321 ttcccgactg ccgttcagca gaaagcgcac ccattccaaa ccgctccaat atgcctcggg
    22381 cagcataccg gcttcagacg gcatatcgtc cgaagcagat aaagtcaggc tgtaacgcgt
    22441 ccctttggtc agaaccatcg ccaaagcata agcaaacggc gcgcgcgttg ccgatacggc
    22501 atatccttcc ggcagcggat cgtccgccgc caaaaccaaa accgacccgc atccctcttc
    22561 caacagtgat gccgcttccg ccaatgccgt ttccacaccg tcggcacaca cggccaatgc
    22621 cgtctgctcg ctcatatccc gcctcaatat cgaccattac cccgccgttg cattgtgcac
    22681 cgacaaaccg aacgaagtcg gcgacacggt ttgcgatttc aacagttcca accacaaatc
    22741 gaaactgcgc gccatttccc cgtcgtgcga ggcataaact atagtggatt aaattttagg
    22801 ggctgtacta gattagcaga tatgttaccc tcgaaatatg aagataacac actgcaaatt
    22861 aaagaaaaaa gtacagaaag aactgctcc
//
LOCUS       DAWEWP010000012        22450 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWEWP010000012 DAWEWP010000000
VERSION     DAWEWP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.012"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            43..606
                     /locus_tag="WGN53_000344"
     CDS             43..606
                     /locus_tag="WGN53_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0139379.1"
                     /translation="MRHLENNGYANVAGLERILAVKTDNYKEKENLLHEIFSKSRIGD
                     TELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSVHERRQEGNAGSGRKQLLDLV
                     RRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGKSGTRLTDEIADGIHIYTCFS
                     RADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            643..1404
                     /locus_tag="WGN53_000345"
     CDS             643..1404
                     /locus_tag="WGN53_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ0139380.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            1459..1800
                     /gene="fdx"
                     /locus_tag="WGN53_000346"
     CDS             1459..1800
                     /gene="fdx"
                     /locus_tag="WGN53_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ0139381.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(2087..2674)
                     /locus_tag="WGN53_000347"
     CDS             complement(2087..2674)
                     /locus_tag="WGN53_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ0139382.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRTATSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            2887..3084
                     /gene="iscX"
                     /locus_tag="WGN53_000348"
     CDS             2887..3084
                     /gene="iscX"
                     /locus_tag="WGN53_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ0139383.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(3152..3553)
                     /locus_tag="WGN53_000349"
     CDS             complement(3152..3553)
                     /locus_tag="WGN53_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0139384.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            3764..4723
                     /locus_tag="WGN53_000350"
     CDS             3764..4723
                     /locus_tag="WGN53_000350"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ0139385.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQRLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            4791..6101
                     /gene="tilS"
                     /locus_tag="WGN53_000351"
     CDS             4791..6101
                     /gene="tilS"
                     /locus_tag="WGN53_000351"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ0139386.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKRAGFVPEALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDILALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRQEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAASDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            6175..6960
                     /locus_tag="WGN53_000352"
     CDS             6175..6960
                     /locus_tag="WGN53_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0139387.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEGKMPSEEIRRLRAVVPEGRIFSVSDGILKKISSLSCADDILALIDI
                     PLGGTLPDKGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLSGIYPQADLEIWLASYKGRVFATALREEKQVVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            6970..7167
                     /locus_tag="WGN53_000353"
     CDS             6970..7167
                     /locus_tag="WGN53_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139388.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLFWMWWKWF"
     gene            7429..7782
                     /locus_tag="WGN53_000354"
     CDS             7429..7782
                     /locus_tag="WGN53_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139389.1"
                     /translation="MKNCKFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            7794..8099
                     /locus_tag="WGN53_000355"
     CDS             7794..8099
                     /locus_tag="WGN53_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139390.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDLVYSSVRPAVKDRAEDAGADDHLDSVRDEHVQNPRENTHKDASNTGAKTH"
     gene            8161..9537
                     /gene="mpl"
                     /locus_tag="WGN53_000356"
     CDS             8161..9537
                     /gene="mpl"
                     /locus_tag="WGN53_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ0139391.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHYL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHAGVGIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRIGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     GKLLDALR"
     gene            9744..10796
                     /gene="bioB"
                     /locus_tag="WGN53_000357"
     CDS             9744..10796
                     /gene="bioB"
                     /locus_tag="WGN53_000357"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ0139392.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVHQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(10875..11508)
                     /locus_tag="WGN53_000358"
                     /pseudo
     CDS             complement(10875..11508)
                     /locus_tag="WGN53_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            complement(11632..11871)
                     /locus_tag="WGN53_000359"
     CDS             complement(11632..11871)
                     /locus_tag="WGN53_000359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139393.1"
                     /translation="MGGCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            11831..12091
                     /locus_tag="WGN53_000360"
     CDS             11831..12091
                     /locus_tag="WGN53_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0139394.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(12154..14013)
                     /gene="ilvD"
                     /locus_tag="WGN53_000361"
     CDS             complement(12154..14013)
                     /gene="ilvD"
                     /locus_tag="WGN53_000361"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ0139395.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHVGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDITNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(14186..15955)
                     /gene="cysI"
                     /locus_tag="WGN53_000362"
     CDS             complement(14186..15955)
                     /gene="cysI"
                     /locus_tag="WGN53_000362"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ0139396.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILSELDDLIGKWAAGRDTDEGFGDFAIRTGI
                     VKPVLDAPVDFWDASKAVPIARA"
     gene            complement(15982..17796)
                     /locus_tag="WGN53_000363"
     CDS             complement(15982..17796)
                     /locus_tag="WGN53_000363"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ0139397.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKASDSLEAAGIQVRRAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDRRFEELGAKRLLERVDADLDFAAAADGWTGRIVARLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(17793..19079)
                     /locus_tag="WGN53_000364"
     CDS             complement(17793..19079)
                     /locus_tag="WGN53_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ0139398.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNLVYPGGNMPWYRGEPLLVIL
                     ENLPGADETGRSAADFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(19118..20041)
                     /gene="cysD"
                     /locus_tag="WGN53_000365"
     CDS             complement(19118..20041)
                     /gene="cysD"
                     /locus_tag="WGN53_000365"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ0139399.1"
                     /translation="MTKTEPNNVQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPAEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(20079..20813)
                     /locus_tag="WGN53_000366"
     CDS             complement(20079..20813)
                     /locus_tag="WGN53_000366"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ0139400.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLDRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIDKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            complement(20829..22280)
                     /gene="cobA"
                     /locus_tag="WGN53_000367"
     CDS             complement(20829..22280)
                     /gene="cobA"
                     /locus_tag="WGN53_000367"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0139401.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGGRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLSELAETAPNPALIVIGEVVGLHEKL
                     AWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
BASE COUNT         4854 a         5821 c         6457 g         5318 t
ORIGIN      
        1 tataaaatcg gcatttcgga tgtaagcaat tttgaaggca gaatgcgcca tttggaaaac
       61 aacggctatg cgaacgttgc cggattggaa cgcatcctcg ccgtcaaaac cgacaattac
      121 aaagaaaaag aaaacctgct ccatgaaatt ttcagcaaaa gcaggatagg cgataccgaa
      181 ttgttcgccg tggacgaaaa ccttgtaaaa cgtttgtttt tatcgcttcg cggcgaaatc
      241 gtgtttccga aaaacgaaac ggcggaatcg gaatttgaaa aaagcgtcca cgaacgcagg
      301 caggaaggga atgccgggtc cggccgcaaa caactgcttg atttggtacg gcgcggacac
      361 cgggaatacc cttacgcgct gccccggctt ttggcgggcg cggcatccta caagccgaaa
      421 aaatcgaaaa tccgcctttt taaagaagca tatttcggca aaagcggcac gaggctgacc
      481 gacgaaattg cagacggcat ccatatttac acctgttttt cgcgggcgga tttggaaaaa
      541 gcctattccg aatatttgga acttttcaaa tccgaatcgg atgccgaagg cagaaagccg
      601 cagtaaggtg caaacagata ccgtacacgt tgaggagcag atatgatggg cgattccgtc
      661 atttattatg tagaacaggc agacaaaccg gtaaaccgtg ccggcgaacg cgcccgcaaa
      721 acattcaaat atttttggcg cgagcttttt tgggaacgcc gccgcatcat tcccgcctta
      781 gattttgcca tggtcaaagc cccttttttc caagacggcg aagacggcga aatttgcgaa
      841 catatgtgga tcgacgatat ttattttgac ggtctttaca tttatggcgt gctgaacaat
      901 gaacccggcg aactgaccaa tgtcgaacaa ggcgaaagcg tttgcgttcc ggttgacgac
      961 atcagcgact ggatgttcgt gtgcaacggc attccctacg gcggctttac catacaggca
     1021 atgcgtggac agatgacgga agaggagcgt accgaacacg atgccgcatg gggaatcgat
     1081 ttcggcgatc ccgggcaggt attgctggtg tatgaagaaa aagaacatcc cgaaaatctg
     1141 gaagagcatc cgatgtgccg gaactgtatt gacgattttc ggcaacagtt gtcccaaaat
     1201 ccggattttc tgcatgagca ggacgaagac ggctatacgc cgcttcatca tgaagccatc
     1261 gcaggaaatg cacttatggt tcaagccatg cttgaatacg gcgcaaatcc tgcctcaaag
     1321 acatcggaag gctataccgc cctcgatttt gcccgcctgg cgggctggca aaatgttgcc
     1381 gacctgctcg aaccgcgaca ttaggcggac agttttccga aaacgaacac aaacactttt
     1441 tacagaaaga caataaaaat gcccaaaatc accgtacttc cacacacgac attatgcccc
     1501 gaaggcgcgg ttatcgaaga cgcgcccgaa ggcaaaaccg tccttgacgt gctgctcgac
     1561 catgatatcg aagtcgatca cgcctgcgaa aaatcctgcg cctgcaccac ctgccacgtc
     1621 atcatccgca aaggtttcga cagtttggaa gaaccgaccg aattggaaga agacctgctc
     1681 gaccaggctt ggggtttgga agccgattca cgcctgagtt gtcaggcggt tgtcgccggc
     1741 gaggatttga ttgtggaaat ccccaaatac accatcaacc acgcgcgcga agaacactga
     1801 aaacaggccg tctgaagccg gcacgcttca gacggcattg ttgcgcggat aaggcgcaat
     1861 cgcccgaaaa caggcgttcg tacaggcgga actttcgatt ctatagtgaa ttaacaaaaa
     1921 tcaggacaag gcggcgagcc gcagacagta ccaatagtac ggcaaggcga ggcaacgctg
     1981 taccggttta aatttaattc actatatatt gatttttatc ggttttctga cggaacggat
     2041 tggtgcggca tccgaggcgg attactcggg cgcgatgcac gggtatttat cggttttgca
     2101 gccggaaaaa ccgccggcgg gttactttca gggcggcggt cgggcggtat gccggatagc
     2161 gttccacttt cgcttctata ttgattttga ttggttttct gacggaatga tccgatgcgg
     2221 catccgggac ggcttgtgtt tcttcctgcc cgcctgccgg atattcctgt acttgcacga
     2281 aaccgaaaga ggtggcggtg cggcggacaa gctcgagata gcgtccttca agctccggac
     2341 aggcggcgga cacgctttct gccgtaaccg tgaagccgcc gcccgacccg gcaaggcgtt
     2401 ccgcaatctg cgtgtagatg cgccaacgtt gggcgcgcaa cattgccgca tcgcaaccgg
     2461 cagccgcttt atccaaggca atcagaccgc gcctgctccg gtagtgaagg caaaccgtta
     2521 ctctggagag gatatgttgc ccgtccaata tttgatacag tttgcgtatc agcagaatca
     2581 ggcggtcaaa ctcctccatc tccgacaggg aatcaggatg gtcggaagcg gtataaacca
     2641 gtttggacaa ttttgcggca aacatattgt tcatcaatct tccttgtcgg ttgaaacccc
     2701 gctcttcggg gcggtagaat cagatttgtt tgggagggac aactcctccc agatcaggac
     2761 gacacatagg ctggtgcttg atgtgttgtc cggcgagttg aaacattcag ccatcctcaa
     2821 ggtgcggcag ttttgccgaa acatattcta cacggcttca atgccggacg ataaaaggaa
     2881 attcatatga aatggaccga cacccggcgt atcgccgaag aactctatga cctgcacggc
     2941 gaaaccatcg atcccagaac cgtgcgcttt acccaactgc gcgacctgat tatggcattg
     3001 cccgaatttg acgacgaccc cgcccgttgc ggcgaacgca tcctcgaagc cgtgcagcag
     3061 gcatggatag acgaggcgga ataagtttcg ggaatgccgt ctgaaatgcg gcggtacgcg
     3121 gttcgtgctt ctgtttgcag cgggaatggt tttaccagtc tccttttttc agcctgtcca
     3181 gttggcggcg gtcgcgcttg gtcggtctgc cgtcgggata ggcggaagtg atgcggctga
     3241 attggtcgag ctgtttgcgc tcttccctca atgttgccgt tttcgcgtcc tcttcataca
     3301 gaagccgcgc ctcggatgcc gggcggcgtt ggtggttcaa acctttaacc ttgattttat
     3361 agggaaggga attgagcgtc aggtcgataa tatcgccgat gtctatggtt ttactgtttt
     3421 tgaccttcga gccgtttact tgaaccctac ccagttcgat gtgcttttgc gcaagggaac
     3481 gggtcttgaa aaaacgtgcc gcccaaagcc atttgtccag ccgcatggcg gaagaatcgt
     3541 gcttgtcttt catacgattt tgtttgaaat aattgaattt gtttcaagtt tagcataaga
     3601 tacgccgcct ataactagta tatatgcact aatccactgt tttccatgct gtccgaacac
     3661 aaaaagaggg tatggaaaag ccgttttgga caataaatta actgcggaat atgcacaaat
     3721 agcgtatgat agcggcagaa tctgttgatg agagcttcat tctatgaaac ctgttttttt
     3781 ggattttgaa caacccatag ccgaactgac caacaaaatc gatgagctgc gtttcgtcca
     3841 agacgagtct gccgtcgata tttcggacga aatacaccgt ttgcagaaaa aaagcaacga
     3901 tttgaccaag tctattttca gtaaacttac acccgcccaa atttcacagg tttcccggca
     3961 tccgcagcgt ccctatactt tggattacat tgaggcactg tttaccgatt ttgaagaact
     4021 gcacggcgac cgccactttg ccgacgatta tgcgattgtc ggcggattgg cgcgtttcaa
     4081 cggacaaagc gtgatggtcg tcgggcatca gaaagggcgc gacaccaaag aaaaaatccg
     4141 ccgcaacttc ggtatgcccc gtcctgaagg ttaccgcaaa gccctgcgcc tgatgaagac
     4201 ggcagaaaaa ttcggcttgc ccgtcatgac ctttatcgat acgccgggcg cgtatcccgg
     4261 catcggcgcg gaagaacgcg ggcagtcgga agccatcggc aaaaacctgt acgaactgac
     4321 acgcctgcgc gttcctgttt tgtgtaccgt catcggcgaa ggcggttcgg gcggtgcgtt
     4381 ggcggtcgcc gtaggcgatt acgtcaatat gctgcaatat tcgacctatt ccgttatttc
     4441 ccccgaaggc tgcgcgtcta ttttgtggaa aacggcggaa aaggcggcgg atgcggctca
     4501 ggctttgggc attaccgctg accggctgca aaggctggac ttggtcgata ccgtcatcaa
     4561 agaaccattg ggcggtgcgc atcgggattt cgggcaaacc atgaaaaacg taaaagccgt
     4621 tttggaaaaa caactgcacg aagcgcaaag catcccgctt gccgatttgc tttcgcgccg
     4681 tttcgaccgc attatggctt acggcaaatt ttcggaacaa taattcaggt agaacaagca
     4741 gcaagcagtt tgtctgaaac tgcttgcttt ttctttatcg ggacggaacc gtgctgactt
     4801 tagatgcgtt tgagcaatgc ttgaaggatt gttttcctca aggtctgaat ggaaaaaaaa
     4861 cagcggtggc attaagcggc ggcttggatt ccgtggtctt gctgcacctg cttgtccgcg
     4921 ctgggaaacg ggcaggattt gtgccggagg cattgcatat ccatcacggc ttgagccccc
     4981 gtgccgacga ttgggcggat ttctgtcaaa actattgcga tatgctaggg gtggggctgg
     5041 aaacggttaa ggtctgcgtg gaaaaaaacg gtttgggcat cgaagcggcg gcgcggcaaa
     5101 agcgttatgc cgcgtttgcc gaaaaaggct ttgacatttt ggcgttggcg caccacaggg
     5161 acgatcaaat cgaaaccttt atgctggcgg tcgcgcgcgg cggcggtttg cgcgctttgg
     5221 cggctatgcc cgccgtccgc ccttttgggg aaaaaggcat catttggcgg cccttgctgc
     5281 ctttttcgcg ccaagatata tgggattatg cccaaaaaca cggtttgccg aatatcgagg
     5341 atgaaagcaa taccgatacg gcttatttgc gaaaccgctt ccggcaccgt attttgcccg
     5401 aactttcggc gcagattccc catttcgggc gacatgtgct gaacaatgtc cgcgctttgc
     5461 aggaagattt ggctttgttg gacgaggtcg tcgttcagga ctgccgttgg gtttgcgggg
     5521 ccggttattt cgatacggcg cggtggctga cgttttcccc gcgccggaaa acccatattt
     5581 tgcggcattt tctgaaggaa aacggcattc ccgtgccgaa tcagaatgcc cttgccgaca
     5641 ttgcccgggt tttgacggag gcaaaaaccg gacgttggaa cttgcaaggc tttgaattgc
     5701 atcattatgc aggcaggctg tttgtgttcc gacaggaaaa aacggataaa ctgcggtttt
     5761 tgaaagacag gcagataagc ggaaatttaa gggaaatatt gacggggcag ggatttgttt
     5821 taaaacgtca tccgtttggg cttcctgagc atcttttgga gcaggacgga attttgagga
     5881 cggtagcggc atcggatacg ttggctgtcg gcggcatcca taaggatgtg aaaaaaatcc
     5941 ttcaggggaa acgggttttg cctgtcctgc gcccaatttg gccgcttgtt gccgacagcg
     6001 gaaaccgtcc attggcgttg gcaaactgtt gtgcggattt ccaatattcg gtttcagacg
     6061 gcattttgcc cgtccatcct gactttccca ttttattttg ataatatcgc aaacagattt
     6121 cggcggcatt cagtcgggta ttgtccggtt gcatatttct aaaaggcttg tgaagtgaaa
     6181 cacatcagtt cgaccaataa tgaacacatc cggcacctgc accgcctgtt gtcgcaagga
     6241 aagttcagac ggcagtatgc ccaaaccgtt ttggagggcg tgcacctgct tcaggttttc
     6301 ctgcaatccg gcgggatgcc ggtcggggta tatattcccg agggcaaaat gccgtctgaa
     6361 gaaatccgca ggctgagggc ggttgtgccg gaaggcagga ttttttccgt ttcagacggc
     6421 atattgaaaa aaatcagcag cttgagttgt gcagacgata tacttgcact gattgatatc
     6481 ccattgggcg gaaccttgcc ggacaaaggc gactgtgtgg ttttggacgg cgtgcaagat
     6541 ccgggcaatg tcggcaccgt cttacggagt gcggcggcgg caggggtagg tacggtcgtt
     6601 ttgggcaggg gttgcgcgga cgcgtggtcg cccaaggtat tgcgcgcggg gatgggcgcg
     6661 catttcttgt cgggcattta tccgcaggcg gatttggaaa tatggttggc gagctataaa
     6721 ggccgtgtgt ttgccaccgc cttgcgcgag gaaaagcagg tggttttgta cggcgaagat
     6781 ttgtgcgaac cgacagcctg ggtgtttggc aacgaaggcg cgggggtcgg taaagcggtt
     6841 ttggacaggg cggacaagtg tgtcaggatt ccgatgcacg atgcaaccga gtctttaaat
     6901 gtcgcgatgg cggcgacaat ctgcctgttt gaacagatgc ggcagcggtc ggcgtattga
     6961 ggaaaagaaa tgccgtctga aaaaatctat tacggcgtat tgattttctt atgtatcgct
     7021 tctatgctgc tgtcgccgtt tttttatgcg ggtgctttga agcccaagaa ggcggcattg
     7081 cggaaggacg ggcagtggaa actcatctta ttgtccaata ccgtggcggc ggcggttttg
     7141 ttttggatgt ggtggaaatg gttttgacag atattgctca aaatcgtgct aatggaatcc
     7201 gaacaaataa agaatttggt aaaaaatttg ttaaatcaac gaattaaagt tttgtggaaa
     7261 acaaaacagc tctaagcaaa tagggcgttt gtcggtaaat acggaagagt tgcggcatta
     7321 tcgggcatct ttaacaagta gtgccggctt ggcaggcaat tggtttttat gggcagtttg
     7381 caaaattgca gatataaaat tgcgaatcgg ttaaagcgtg aggataccat gaaaaattgc
     7441 aaattttttt atgatccgac aagggcaatc tatgatagcg gtgcagatta cttaactagg
     7501 gaaaaacata gattagtcgt tatcgcaaat agtgcttggg ggctattgct taatttatct
     7561 tgttattatg acgaggtttt ggaaaagcgg aaaataccgt tcggcaaaca ggaaattgat
     7621 gacgatatgg acaaagtgtc cgcccataag cggaagttta aagatatttc tgaaatcaaa
     7681 gtaggggatg gttgggaata cccgttcaat tatgagcagg gaatgaaaga attagatgaa
     7741 gtgctattga aatacattcc cttttttgaa gaagaacgat aaaggaggtt gatatgcgcg
     7801 tatctaaaat aattggaagt atgttgcttg ttacagcggt tcagaccgta ttttcggcaa
     7861 atgtttacgc gtgccgccat aatggtaaaa ccagttacag ccaaactccg ggaaaacatt
     7921 gtaccaacgc gggtttgggg cgggacctgg tgtacagttc ggtcaggcct gccgtgaaag
     7981 acagggcgga agacgcaggg gctgacgatc atttggattc ggtgagggac gaacacgtcc
     8041 aaaatccgag agagaatacg cataaggatg cttcgaatac cggtgcaaag acgcattgat
     8101 tgaaaccgaa tcagccatga cggcgttgaa taatagaatt tgaacgatta gggaactctg
     8161 atgaaacaca tccacattat cggtatcggc ggcacgttta tgggtgggat tgccgccatt
     8221 gccaaagaag cagggtttga agtcagcggt tgcgatgcga agatgtatcc gccgatgagc
     8281 acccagctcg aagccttggg cataggcgta cacgaaggct tcgacgcggc gcagttggag
     8341 gaatttaaag ccgacgttta cgtcatcggc aatgtcgcca agcgcgggat ggatgtggtt
     8401 gaggcgattt tgaaccgcgg gctgccttat atttccggtc cgcaatggct ggctgaaaac
     8461 gtgctgcacc atcattgggt actcggcgtg gcggggacgc acggcaaaac gaccaccgcg
     8521 tctatgctcg cgtgggtttt ggaatatgcc ggactcgcac cgggcttcct tattggcggc
     8581 gtaccggaaa atttcggcgt ttccgcccgc ctgccgcaaa cgccgcgcca agacccgaac
     8641 agccaatcgc cgtttttcgt tatcgaagcc gacgaatacg acaccgcgtt tttcgacaaa
     8701 cgctccaaat tcgtgcatta ccgtccgcgt accgccgtgt tgaacaatct ggaattcgac
     8761 cacgccgaca tctttgccga cttgggcgcg atacagaccc agttccacta cctcgtgcgt
     8821 accgtgccgt ctgaaggctt aatcgtctgc aacggacggc agcaaagcct gcaagatact
     8881 ttggacaaag gctgctggac gccggtggaa aaattcggca cggaacacgg ctggcaggcc
     8941 ggcgaagcca atgccgacgg ctcgttcgac gtgttgctcg acggcaaaac ggccggacgc
     9001 gtcaaatggg atttgatggg caggcacaac cgcatgaacg cgctcgccgt catcgccgcc
     9061 gcgcgtcatg ccggtgtcgg cattcagacg gcctgcgaag ccttgggcgc gtttaaaaac
     9121 gtcaaacgcc gcatggaaat caaaggcacg gcaaacggca tcaccgttta cgacgacttc
     9181 gcccaccacc cgaccgccat cgaaaccacg attcaaggtt tgcgccaacg tatcggcggc
     9241 gcgcgtatcc tcgccgtcct cgagccgcgc tccaacacca tgaaactcgg cacgatgaag
     9301 tccgcactgc ctgtaagcct caaagaagcc gaccaagtgt tctgctacgc cggtggcgtg
     9361 gactggaacg tcgccgaagc cctcgcgcct ttgggcggca ggctgcacgt cggcaaagac
     9421 ttcgatgcct tcgttgccga aatcgtgaaa aacgccgaag caggcgacca tattttggtg
     9481 atgagcaacg gcggtttcgg cggaatacac ggaaagctgc tggacgcttt gagatagccc
     9541 gggcgatgcc gtctgaaagc ccttcagacg gcatcgtccg gctgcgcggc acaaaggcgg
     9601 aaaaaccgtt tgccccgtat tttcaaacgc gttacacttg ccgccgctgt tttcagccat
     9661 ttgattaccc gcaaccgccg tcattgcgcc ggcggtttgc ctgtcagcgt cattgcgccg
     9721 ctgtaaatac gaaagaacac attatgaccg tatcccccgt cgccttgcgc cgtaagaccg
     9781 agtgcaagcc tcatcccacc gcgcgctatt ggaaaaaatg cgatgtcgag gcacttttcg
     9841 gactgccgtt tttagaactt gtccatcagg cggcagaagt ccaccgccaa aatttcaacc
     9901 cgcgcgaaat ccagctttcc acgctgttgt ccatcaaaac cggcggttgt cccgaagatt
     9961 gcgcctactg tccgcaatcg gcgcaccaca ataccaatct gggcaaagag cagatgatgg
    10021 atgtggatga aatcgtcgaa aaagccaaaa tcgccaaatc gcgcggcgca agccggtttt
    10081 gtatgggcgc ggcatggcgc ggccccaaac ccaaagacgt ggagacggtt tccgcaatca
    10141 tcaaagccgt caagggcttg ggtatggaaa cctgcggcac gttcggtatg ctcgaagaag
    10201 gtatggcgga agacttgaaa gaggcgggct tggattatta caaccacaac ctcgacaccg
    10261 accccgaccg ctacaacgac atcatccata cacgccggca tgaagaccgt atggacacct
    10321 tgggcaaagt ccgcaacgcc ggtttgaaag tctgctgcgg cggcatcgtc gggatgaacg
    10381 aaacccgcgc cgaacgcgca gggctgattg ccagcctcgc caacctcgac ccgcagcccg
    10441 aaagcgtgcc gattaaccgg ttggtcaaag tggaaggtac gccgcttgcc gatgccgagg
    10501 atttggactg gacggaattt gtccgcacca tcgccgtggc gcggattacg atgccgcaaa
    10561 gttatgtccg actgtcggca gggcgcagca atatgccgga agcgatgcag gcgatgtgct
    10621 ttatggcggg cgcgaactcg attttttacg gcgacaagct gctgaccacg ggcaatccgg
    10681 atgaagacgg cgacagaatc ctgatggaaa agctcaacct gtatccctta cagtttgagc
    10741 tggaaggcga ggtcgccgaa gtggaaaaag cctctgggat taaagtggat tattgacgat
    10801 tgcaaaaatg ccgtctgaaa cccgaaaaaa ggctttcaga cgacatttgt tcggacggta
    10861 tttccaatat ctttttaccg gcgcgtgatg ttgccgtcgg gcgagacatc cagcccgttc
    10921 ccgttgggga agtcgctgcg gttcagataa ataatccgcc cgataacggt ttttcagggt
    10981 cgattttggg gattttatcg tcggcttggg taatagggat gatagttccg gaaataaatt
    11041 taccctgttt gttggtgata attttgaaaa tcggcgcaat gccgctgatg ccggaggtgt
    11101 tgattgcgcc gtaggtggca aagttgccgc cgctgtaaga gatgaagcgg tcgcggtaaa
    11161 gttcgacggc gcgtgtaacg tgcggcccct gcccgaatac gacatccgcg ccggaatcga
    11221 cggcaagccg tgcaaactcg acgacgttgc ccctgttttc cccatagaag atttcggtat
    11281 cgaacggcag gtgttccgcc tgtttccctt cccgccgccg tggaacatta caatgacgat
    11341 gtcggctttc tgtttggttt tggtaatccg ttttctaact ttggcataat cgttcagttt
    11401 gacggcggca aggttggggg cgaaggagac gaagccggat cttacgccgt ttttcttcag
    11461 gatggcggtt tcaaacctgt tttcgatgcc cgaatatttg atgttcaatt cgtcaaggtt
    11521 cgccctttat gccgtttccg caccgcaaac cgccggggtc aagccctcgg cgcatcctgc
    11581 cggaacggaa tccccgtgct gccgattgac ggttcaaacc ccgcgcccgt ttcaaatacc
    11641 ggcgatgtgg acggacagga tgcgcctgac gaaaagacag ccgataccgt ttccattatc
    11701 ggcgtgggcg acattatgct cggcagcaat tatccggtcg attacctgcc cgataccaat
    11761 attctgaaaa acgtcgaatc tgccttgcaa gacgcggaca ttaccgtcgg caacctcgaa
    11821 ggcacgctgt ttgacgaagg cggtacgccg aaaaaatgtg caacccccca aaatatgcta
    11881 tgcattccga acgccctccg catacgggca ataccttgcc gacgcgggat tcgactacct
    11941 cagcttcgcc aacaaccaca gcaacgactt cggcgcgcaa ggcatcacgg caacggcggc
    12001 gagcggcagc tcttttacat actcgatcgc gctaaagccg ctgccgataa cgaggccaaa
    12061 attgcggaaa ataccgccat cgcccagata aatttgtcca tcatcagacc tttactgttc
    12121 agacgagaca gcatttgccg cacgttttgg ggcttatctt tcgatttgcg ctacgtcgcg
    12181 caccgcgcct ttgtcggcgg aagtcgccat cgcgccgtaa gcacgcaggg ctgcggagac
    12241 gtagcggtcg cggttttccg gtttccacgc tttgctgccg cgcgcttcca tttcggcacg
    12301 gcgggcggca agctcttcat cggaaatggc aaggtggatg ctgcggttgg ggatgtcgat
    12361 ttcgacggta tcgccttcgt gtaccaaacc gatcgcgcca ccttccgccg cttcgggcga
    12421 ggcgtgtccg atggacaaac ctgatgtgcc gccggagaag cgtccgtcgg ttaagagagc
    12481 gcaggctttg ccgaggcctt tagatttcag gtaggaagtc ggatacagca tttcctgcat
    12541 gcccgggccg cctttcgggc cttcgtagcg gatgatgacg atgtcgccgg cgacgatttg
    12601 gttgcccaaa atgccttcta ctgcgtcttc ttggctttca aacacgcggg cgcggccggt
    12661 gaatttgagg atgctctcgt ccacgcctgc ggtttttacc acgcagccgc gctcggcgat
    12721 gttgccgaac aagaccgcca aaccgccgtc ttgcgagtag gcgtgtgcca cgtcgcggat
    12781 acagcctttt tcgcggtcga ggtcgagggt tttccacata cggttttgcg agaacgcttg
    12841 ggtggtgcgt acgccgccgg gcgcggcttt gaagcgttcg atggcgcggg tgttttcagg
    12901 gttggttatg tcccattttt caatcgcgtc tttcagcgtc ggtgcgtgga tagtgtgcac
    12961 gtcggtgtgc agcttgcccg ctttgtccaa ttctttcagg atggcgaaaa tgccgccggc
    13021 gcggtgcacg tcttccatat agtagtcgtg gttgttgggt gcggttttgc agatgcaggg
    13081 cacaacgcgg cttaagcggt caatgtcggc cattttgaaa tcgacaccgg cttcgttggc
    13141 aacggccaac aggtgcaaaa tggtattggt gctgccgccc atcgcaatat ccatcgtcat
    13201 agcgttttca aacgcttttt tggtggcaat gctgcgcggt aacacggttt catcgttttg
    13261 ctcgtaatag cgtttggtga tttcgacaat catacggccg gcttcgagga acagttcttt
    13321 gcggccgacg tgggtcgcca aatacgaacc gttgccgggt agggacagac cgagtgcttc
    13381 ggtcaggcag ttcatcgagt ttgccgtaaa catacccgaa cacgagccgc aggtcgggca
    13441 ggcgttttgt tcgacttcct cgacttgccg gttgctgaca ttgtcgtccg ccgattcaat
    13501 catcgcgtca atcaagtcca aacggcgttc gggttggatg tttgccacgc cgataacttt
    13561 acctgcttcc atcggaccgc cggaaacgaa gatggtcggg atgttcaggc gcatcgcggc
    13621 aatcagcatg cccggggtga ttttgtcgca gttggaaatg cacaccagcg cgtcggcgca
    13681 gtgggcattg accatatatt cgatggagtc ggcaatcaaa tcgcggctgg gcagggaata
    13741 cagcataccg ctgtgtccca tagcgatgcc gtcgtcgatg gcgatggtgt tgaattcttt
    13801 ggcgatggcg cccgcctttt cgatttcgcg ggcaaccagc tggcccatat tgtgcaggtg
    13861 gacatggccg ggcacgaatt gggtgaagga gttggcaacg gcgatgatgg gcttgccgaa
    13921 gtcggtttcc atcacgccgg tggcgcgcca caatgcacgt gcgcccgcca tattgcggcc
    13981 gtgggtggag gttttggagc ggtattctgg catagtgtgt ttccttgtct gctatgccgt
    14041 ctgaacggca aaaggctttc agacggcatt tttgttgtgt tttgaaatga aaaaaaaggt
    14101 attttatacc aagtatcgga attttgcgga atggaaacgc gtgcggcaaa aaaggacatc
    14161 ccccgcagga atgcggggga cggtttcagg cgcgcgcaat cgggacggct ttggatgcgt
    14221 cccaaaaatc gacgggcgca tccaacacgg gtttgacgat gcccgtccgt atggcgaaat
    14281 cgccgaaacc ttcgtcggta tcgcgtcccg ccgcccattt gccgatcagg tcgtctaatt
    14341 cggaaaggat ttccggcagg gtaatgtttt ctttgtagag tcgggggatg cgtacgcctt
    14401 cgcggtcgcc gccgatgtgg aggttgtagc gtccgacggc tttgccgacc agtccgattt
    14461 ccgccaacat cgcccgtccg cagccgttcg ggcagccggt aatgcgggta acgatgtagt
    14521 cgtccgacgt gccgtgtttc gccataatct tatccagctc gccgatgaag tccggcagca
    14581 cgcgttcggc ttccgccatt gccagcgggc aggtcgggaa ggaaacgcac gacatcgcat
    14641 tttcacgcag tttactgaca tcgttgcgga ttaatccgta tgttcgggca agttcttcga
    14701 tttttgcttt gtctgcttcg gcgacatttg ccacgatgag gttttggttg gcggtgatgc
    14761 ggaaatcgcc tttgtggatt ttggcgattt ccaacacgcc ggtcagcagc tgtttcccgc
    14821 cttcgtcaac caaacgcccg ctttcgatga aaagggtcaa atgccagttg ccgtctatgc
    14881 ctttcaccca gccgatacgg tcgccgcgcc cggtaaattt gaacgggcgt atgggttcga
    14941 acggcatacc catacggcgt tcgacttccg cgcggaagtt gtccaagccc atattttgaa
    15001 tggtgtagcg ggtgcgggcg tttttgcggt cgctgcggtt gccgaagtcg cgctgcgtgg
    15061 ttaccaccgc ttcggcggcc ttcagcgcgt gttccggagg cacgaaaccc agttccagtg
    15121 aaatgttcgg ataggttttg gtgttgccgt gttccatcga aagcccgccg cctgccaaaa
    15181 cattgaagcc ggcaagctgt ccgttaccgt ctgaaacggc gacgaaatcc aaatcgttgc
    15241 cgtagcagtc cacatcgttc aagggcggga tgacgactgc ggttttgaat ttgcgcggca
    15301 gataggtttt gcccaaaatc ggctcgtctt cttgaaggaa gtcgtcggaa ctttgaactt
    15361 ttttgccgtc cacccacaca tccagataac cgcgcgtgcg cggcagcaag tgttcggaaa
    15421 tctttttcgc gtattcgtaa gcctgccggt gcagttcgga ctcgatcggg ttggacgtgc
    15481 aaagcacgtt gcggttcata tccgccgccg tggcgatgga atccaaaccc agtttgtgca
    15541 agaggcggtg catcgtctgc aacttggctt tcggcacgcc gtgaaattgg aaggtttgcc
    15601 ggttggtcag ccggatggag cggtaatgac tgttttcccg ggcaaatttg tccagttcta
    15661 tccattggga cggtttgatg atcccgcccg gcagccggca gcgcaaaagc ataaatttca
    15721 agggctcgag ttttgcctcg gcgcgttcgg cgcggatgtc gcggtcgtcc tgctcataca
    15781 taccgtggaa gcggatgagt tggaagttgt cgcctttgaa gccgcccgtg agcgggtctt
    15841 tcaaatcgtc caaaatcgtg ccgcgtaaaa aattgctttc ggttttcaga cgttcgttgt
    15901 cggatagcgg tttttcttgc cacgccaaac cttttgtctt ggtctgtacg gtcattttgt
    15961 gttcctcccg attatattta atcaataaac atcacgctga tagcgttttt cttcgcgcag
    16021 catatccaaa tattcttctg cgccctcttc gtccaaatgt cctgccccga taatcacatc
    16081 cagcaaggcg gcttccacgt cttttgccat ttttgccgca tcgccgcaca catagatatg
    16141 cgcgccttcc tgcagccatt gccaaagtcc ttccgcctgt tcgcggattt tgtcctgcac
    16201 atagattttt tcttcctgat cgcgggacca ggcgaaatcg tacctgtgca ggaaaccgtc
    16261 tttggcaaac tgctgccatt cggtttgata gagaaaatca cgggcaaaat gcggattgcc
    16321 gaaaatcagc cagtttttgc cttccgcatt ttctgcggca cgttgttgga cgaaggcgcg
    16381 gaacggtgcg acgccggtgc ccgagccgat catcacaatc ggcttgcggc tgtcttcggg
    16441 cagcctgaag ccgtcgttgc gttccacaaa cacgcgcacc gtgccgtcct cttccagccg
    16501 gtcggcaagg aaacccgatg cgccgcccgc cctggcgcgc ccttcgtgtt cgaaacgcac
    16561 cgcgccgacg gtcaggtgca cttcgtcccc cacttccgcc tgcgacgagg aaatcgaata
    16621 caggcgcggc gcaagcgggc gcagtaggcc ggcgaattgt tccgccgtca gttttgccgg
    16681 gaagcggtgc agcacatcgg caatcggcgt gctttgcaca aagccttgca aaacggcgtt
    16741 gtcggcagca atacggtcga gttcgtcatc atcggcgaac ggggcatagc ctttgacaaa
    16801 ggcgggggtg ttttgcgtga gttcaaaatg ggataacagt gcggaggcaa ccggcagggt
    16861 ttttccgccc gcctgtattt ccgttgccgg atcgatgccg agcaggtcta ggatttccct
    16921 gaccagtgcc ggatcgttgt caaaccaaac gccgagcgcg tcgcccggga ggtagtgcaa
    16981 atccgaaccg ctcaaatcga tttcgatgtg gcgcacgtct ttatcggatt ggcgggcggt
    17041 gattttctga ttggccagca gggcggcggg aaaggggtct gccttgcagt acctgccatc
    17101 cggtgccgtc tgaaggccgg cggggggcgt tgtctgcggc gcgggcgttg cccggttttt
    17161 tgcggcttct tcttttaagc gcgcgacaat gcggcctgtc catccgtctg cggcggcggc
    17221 aaagtccaaa tccgcatcaa cgcgttcgag caggcgtttt gcgcccaatt cttcaaaacg
    17281 ccggtcgaaa tctttgcccg cccggcagaa attcggatag gaactgtcgc ccaaacccag
    17341 tacggcaaat tggagtttgt ccaatttcgg ggcttttttg ccgttcagca gtttgtgcag
    17401 cacgacggct tcttccggcg gttcgccttc gccctgggtg gacgtaacca gcagcaggcg
    17461 gcgttcgtcg gcgatgtttt tcgccttata gtctttcagt tcggcgcgcc tgacttggat
    17521 gccggcggct tccaggctgt ccgacgcttt gtcggcaacg gatttcgcat tgccggtttg
    17581 cgaggcggaa aggacggtta cggaaaaagg ttctgccgcc ggcaatgccg tctgaagcgc
    17641 gggcagccct gcggatgccc cgtttcctgc ttttgcccaa gcgtagccgg acagccacgc
    17701 ccattgtgcc gcgtccagcc ccgacaggag ctgcgtgatt tcgggcggca gaggcggtaa
    17761 tggcggattt gtgttctgca tatcgtgttc actcataaaa tcatacctgc cgcaacagtg
    17821 ccgtatgtcg cttcgtctat caggataaac gaaccggcgg cggtgttttc cgcataaggc
    17881 gttgccgtaa cgggtttttg aaggttgatg cggactttgg cgatgtcgtt catcttcaag
    17941 gattccgcgc cggcctcttg ttccagcgtg cggacatcta aaacgctttc aatttccccg
    18001 acttttgccg gcacggtttg cgtgccgtgc ttgagcaggt atttgcgcgc ggtgttgagc
    18061 ggacgttcgt caaaccagca aagcgtggct tccagatgtt tttgcggggc gagcggggaa
    18121 tttttatcga caaaaaggtc gccgcgcgaa acatcgatgt cgcggtccag ccggagggtt
    18181 gccgcctcgc cggcaaaagc ctgcgccact tcccctttcg gcgtgatgat ttcggacact
    18241 tcggcggtca gcccgttcgg ttcgatgcgg acggtttgcc cgacggcgac cgaaccgcgt
    18301 tcgatgcgcc cctgatagcc tcggaaatca tcggccttgt cggcatcttg gcggacgacc
    18361 agttggacgg ggaaataaaa atcggcagcg gaacgaccgg tttcgtccgc ccccggcagg
    18421 ttttccaaga tgaccagcag cggttcgccc cgataccacg gcatattgcc gccggggtaa
    18481 acgaggttgt cgcccaagag tgcggacacc ggtacgaaat gcacgtcttt caaaccgagc
    18541 tgttcggcaa gtcggcggta tgcctccaca atggcgttga atttgtcttc gctgtaatcc
    18601 agcaggtcca ttttgttgac cgccaccaca atatgcgggc agttgagttg gcggaggatg
    18661 gcggaatggc gtttggtctg cggcagaagc tgcaagggct gcgcgccgaa atccagttgg
    18721 gatgcgtcaa ccagcacgac tgccgccgaa gcggtgcttg cgcccgtaac catattgcgc
    18781 gtgtattgtt cgtgccccgg cgtgtcggcg atgatgaatt tccgtttcgc cgtggaaaaa
    18841 tagcggtatg ccacatcgat cgtaatgccc tgttcgcgtt cggcttccag tccgtcggtc
    18901 aggatggaga agtctatggt ttctttcaaa cctttgcttt tgccggattc caaggttttg
    18961 atttggtcgg acagcagggc tttgctgtcg tagagcagtc gtccgatcag ggtgcttttg
    19021 ccgtcatcga cgctgccggc ggtaatgaag cggagcgggg cctggtgttg tgccgtcata
    19081 ttttcttcct catatctgct taaagggttt ttgaaattta gaaatagcct tcttttttgc
    19141 gtttttccat tgccgcctcg cttgcctgat cgtctagccg ggtcgcgctg cgttcggaaa
    19201 tgtcggcaac cgccgtttcc ctgataatct ccgccggcgt ggacgcggtg ctttctaccg
    19261 ggcaggtgca gctgatgtcg ccgacggtgc ggaagcggac atcaaggatt tcggaggttt
    19321 cagacggcat tttcggggtg agcggcgtta cagggaccag cagccccctg cgtctgacca
    19381 cttcgcgcct gtggctgtaa taaatcggcg gcagctcgag gttttcgcgg gcgatgtatt
    19441 gccagatgtc gagttccgtc cagttggaaa tcgggaagac gcgcatattt tcgcctttgt
    19501 gcagcctggt gttgtacagc gaccacagct cggggcgttg cgccttcgga tcccattgtc
    19561 cgaactcgtc gcggaacgag aaaatccgtt ctttggcgcg ggctttttct tcgtcgcgcc
    19621 gcgcgccgcc catcagcgcg tcaaagccgt ttgcctcgat ggtttccaac aaggtaaccg
    19681 cctgtgccgc attgcgcgaa tcggtttctt tgcgtaagac caccgtgcct ttagcgatgg
    19741 agtcttccac gcgccccact atcaggcggg cattgagttt tgccgcctgc gcgtcgcgga
    19801 aggcaatcac ttcggggtag ttgtgccccg tgtcgatatg caccagcggg aagggcagtt
    19861 ttaccggcct gctgcccagc cggaaggctt tgcaggcgag ggcgagcagg accacggaat
    19921 ctttgccgcc ggaaaagagc agggcggggt tttcgcattc tgccgccact tcgcggataa
    19981 tgtggatgga ttcggattcc aaccagtcga gttggacgtt gttcggttcg gttttcgtca
    20041 tacgatattc cttatttctt ctgtttgatc cttatgaatt atttgtgcag cccgcattct
    20101 ttgctgtttt tatcttccca ccaccaccgc ccggcgcgga tgtcttcgcc ggccttgacg
    20161 gggcgggtgc aggggtcgca gcctatactg ggaaagcctt gccggtacaa atcgttgtaa
    20221 ggcacattgt tggcaaggat gtatgcccac acgtcgtgtt ccgaccagtc gaaaatcggg
    20281 ttgtatttat cgatgccgcg cccggcatca tattcggcaa acggcagttc cgtgcgtgtg
    20341 gcggactgtt cgcggcgttg cccggtaagc caagcatccg ccccggcaat ggcgcggtcg
    20401 agcggttcgg tttttcggat gcggcagcat tcgcggcgcg cttcaacgct gtcgtaaaag
    20461 gcaaacctgc ctttgctttc cacataatgc agggcatctt cccgtatcgg ttggaaacgc
    20521 ttgatttgca tatgcggata aacacgttca atcctatcca acaggtttaa agtttccgcg
    20581 tgcagcagcc ccgtatccaa agtaaaaata ccgatattga ggttttctcc ggcgataagg
    20641 tcggtaatta ccatatcttc cgcagcaagg ctgctggcaa accgtgcatc ccggtggctg
    20701 ccggcaatcc ggtgcaggcg ttgtttgagg gtttccgttt tttccgccaa agccgtttca
    20761 ctgccatttt caatcggcgg aattttccat aaagcaggtt tgaaaatttt cattttgtcc
    20821 gccttgcttt atgcggcttg ttgttcttgt ccggtgccca gtccgtccaa tgcaaaatac
    20881 gcgtgttcgg ccggattgct ttcttttttg gcgttttcgc cgaaccaagc cagtttttcg
    20941 tgcagcccca ccacttcgcc tatgacaatc aatgccggat tcggcgcggt ttcggcgagt
    21001 tcggaaaggt tggcgagcgt gccggttgcg gttttttgag ccggcagcgt gccttggctg
    21061 ataacggctg ccggcgtgtc gggcgagcgt ccgtgctgtt gcagccgttc ggcaatcagg
    21121 gcggctttga gcgcacccat ataaatcacc aaggtctggc ggctgcgggc gagggtctgc
    21181 cattcgatgt cgggcgcatc cgccttgcgg tggccggtta cgaaaaccgc actttgggca
    21241 taatcgcggt gcgtgagcgg gatgccggca taggcggtcg cgccgacggc ggcggtaatg
    21301 ccggggacga ccgaaaacgg gatctgatgg cgtgccaagg tttccaattc ttcgccgccg
    21361 cgtccgaaca cgaaagggtc tccgcctttt aaacgcacca cgcgcctgcc ttcgcgggcc
    21421 agcctgacca taagcgcatt ggtgtcctct tgcggggtgc ggccgccccg ggcgcgcttg
    21481 ccgacaaaaa tccgttccgc atcgcggcgg acgagggaca gtatgccgtc tgaaaccagc
    21541 gcgtcgtaaa gcaccacgtc tgcctgctgg atttcctgca gccctttgag cgtcagcagc
    21601 cccgcatcgc cgggacccgc accgaccaaa gacacaaaac cgccttgata attttgttcc
    21661 aactgctttg ccaactcctg ttcggcttgg gcggtttgtt gatttttaac gagggcggca
    21721 aaacggccgt tgaactgctt ttcccaaaaa cggcggcgtt cggtaacgga tttcagtttg
    21781 gctttgaccg catcgcgcca tcttccggag atttccgcca tatcgcccaa agacggcggc
    21841 agcagggctt ccagcctttc acgcagcagt cgggcgagga cgggcgcgct gccggagctg
    21901 gaaacggcaa tctgaaccgg gttgcggtcg ataaccgacg ggaagatgaa gctgcaatgg
    21961 tcgcggtcgt ccaccacgtt gaccggcttt tggcagcttt cggcaagatg gaaaacgcgc
    22021 cggttgaggg cttggtcgct gcttgccgca atgatgagga aaaccgtgcg gatgtgttcg
    22081 gcacgaaatt cttcggcaag ccacaggatt ttgttttccg ccgccaacgc ggagagttcg
    22141 gcattcaggt gttttgcggc aaccctgacc tctgcgcccg ccttcagcag caggctgatt
    22201 ttgcgtgcgg cgaccgcgcc gccgcctacg accaatacgg ggcggccggc gaggttggcg
    22261 aaaataggga aataattcac tggctgactc ctttgctgtt tgcccgcacc ttgtttccga
    22321 tacggtgcgt cgcggcattt ttgtcggaat gcgggtcatt ttagacaaaa ggattttccc
    22381 ggttaaataa taaaaaggca tttgttagaa gctgaaagct atatgagggc ggctgcggat
    22441 gcggcggttt
//
LOCUS       DAWEWP010000013        22287 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWEWP010000013 DAWEWP010000000
VERSION     DAWEWP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.013"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..335
                     /locus_tag="WGN53_000368"
     CDS             <1..335
                     /locus_tag="WGN53_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0139402.1"
                     /translation="EYDENSFIGKWYDDGVWDDEEYWKLENALIEVRRKYPYPMDIPR
                     DIVIGIGTIIEFLMVPNWKLFTIKSSPWLPKSVKIDERYERFRVMLRYIFTEIDIVNV
                     RFDYYNKK"
     gene            419..913
                     /locus_tag="WGN53_000369"
     CDS             419..913
                     /locus_tag="WGN53_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139403.1"
                     /translation="MENRFKKIVKDILSFAQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMEMFKMSVLEDWERSGKPLDWKKWQRQYRSEAFELVKKLNR
                     SKQE"
     gene            1033..2166
                     /locus_tag="WGN53_000370"
     CDS             1033..2166
                     /locus_tag="WGN53_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139404.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHRENYETTGEYRVVWQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLENLEKEVRRYAEAAARRSGGRRDLSH"
     gene            complement(2253..2528)
                     /locus_tag="WGN53_000371"
     CDS             complement(2253..2528)
                     /locus_tag="WGN53_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139405.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLMCISVFWIVVFNL"
     gene            complement(2545..3312)
                     /locus_tag="WGN53_000372"
     CDS             complement(2545..3312)
                     /locus_tag="WGN53_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0139406.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNLEPMTGFE
                     HTVTFDFQGIKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPE
                     YEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNYHRYGENDYSTSVGSCINGFTVR
                     YYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(3724..3999)
                     /locus_tag="WGN53_000373"
     CDS             complement(3724..3999)
                     /locus_tag="WGN53_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0139407.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(3996..5279)
                     /locus_tag="WGN53_000374"
     CDS             complement(3996..5279)
                     /locus_tag="WGN53_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0139408.1"
                     /translation="MTVSKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(5433..6851)
                     /gene="glnA"
                     /locus_tag="WGN53_000375"
     CDS             complement(5433..6851)
                     /gene="glnA"
                     /locus_tag="WGN53_000375"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ0139409.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(7125..7502)
                     /locus_tag="WGN53_000376"
     CDS             complement(7125..7502)
                     /locus_tag="WGN53_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139410.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVLVDSNTATFGDADSRTDNSA
                     WFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPSYAE
                     WNYVIPGTLGEAQYKFVCNGYPR"
     gene            7673..8482
                     /gene="aroE"
                     /locus_tag="WGN53_000377"
     CDS             7673..8482
                     /gene="aroE"
                     /locus_tag="WGN53_000377"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ0139411.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGIGLTNDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            8484..9185
                     /gene="mtgA"
                     /locus_tag="WGN53_000378"
     CDS             8484..9185
                     /gene="mtgA"
                     /locus_tag="WGN53_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ0139412.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDAHFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(9388..9463)
                     /locus_tag="WGN53_000379"
     tRNA            complement(9388..9463)
                     /locus_tag="WGN53_000379"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9428..9430),aa:Phe,seq:gaa)
     gene            complement(9654..10388)
                     /gene="lptB"
                     /locus_tag="WGN53_000380"
     CDS             complement(9654..10388)
                     /gene="lptB"
                     /locus_tag="WGN53_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ0139413.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(10462..10992)
                     /gene="lptA"
                     /locus_tag="WGN53_000381"
     CDS             complement(10462..10992)
                     /gene="lptA"
                     /locus_tag="WGN53_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ0139414.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(10973..11554)
                     /gene="lptC"
                     /locus_tag="WGN53_000382"
     CDS             complement(10973..11554)
                     /gene="lptC"
                     /locus_tag="WGN53_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ0139415.1"
                     /translation="MKIRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(11551..12087)
                     /locus_tag="WGN53_000383"
     CDS             complement(11551..12087)
                     /locus_tag="WGN53_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0139416.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPMMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(12152..13126)
                     /locus_tag="WGN53_000384"
     CDS             complement(12152..13126)
                     /locus_tag="WGN53_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ0139417.1"
                     /translation="MAENGKYLDWAREVLHTEEEGLREIATELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            13223..14278
                     /gene="tal"
                     /locus_tag="WGN53_000385"
     CDS             13223..14278
                     /gene="tal"
                     /locus_tag="WGN53_000385"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ0139418.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVQAACDVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRVQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALETQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGADEAQAQLAETAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            14432..14722
                     /locus_tag="WGN53_000386"
     CDS             14432..14722
                     /locus_tag="WGN53_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139419.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            14739..15626
                     /gene="gluQRS"
                     /locus_tag="WGN53_000387"
     CDS             14739..15626
                     /gene="gluQRS"
                     /locus_tag="WGN53_000387"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ0139420.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQAAAVHGADGFVYNGRCRNPQQRPALQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMGKVPKHAITTP"
     gene            complement(15696..16706)
                     /gene="dusA"
                     /locus_tag="WGN53_000388"
     CDS             complement(15696..16706)
                     /gene="dusA"
                     /locus_tag="WGN53_000388"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ0139421.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQCLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(16737..17228)
                     /locus_tag="WGN53_000389"
     CDS             complement(16737..17228)
                     /locus_tag="WGN53_000389"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ0139422.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(17302..18060)
                     /locus_tag="WGN53_000390"
     CDS             complement(17302..18060)
                     /locus_tag="WGN53_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0139423.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRNPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(18131..18997)
                     /locus_tag="WGN53_000391"
     CDS             complement(18131..18997)
                     /locus_tag="WGN53_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0139424.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(19046..19324)
                     /locus_tag="WGN53_000392"
     CDS             complement(19046..19324)
                     /locus_tag="WGN53_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0139425.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(19324..19614)
                     /locus_tag="WGN53_000393"
     CDS             complement(19324..19614)
                     /locus_tag="WGN53_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ0139426.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(19617..20147)
                     /locus_tag="WGN53_000394"
     CDS             complement(19617..20147)
                     /locus_tag="WGN53_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ0139427.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            20455..>22287
                     /locus_tag="WGN53_000395"
     CDS             20455..>22287
                     /locus_tag="WGN53_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ0139428.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPS"
BASE COUNT         5500 a         6021 c         5528 g         5238 t
ORIGIN      
        1 aagagtatga cgaaaattca tttattggca aatggtatga tgacggggtg tgggatgatg
       61 aagaatattg gaaattggag aatgctttaa tcgaggttag gagaaaatat ccttatccga
      121 tggatatacc aagggatatc gtgattggaa tcggtaccat tattgagttt ttaatggttc
      181 caaattggaa actttttaca attaaatctt ccccttggtt gcctaaaagt gtcaaaattg
      241 atgagcgtta tgaaaggttc agagtaatgc tccgttatat ttttaccgag atagacatag
      301 tcaacgtgcg atttgattat tacaacaaaa aatagcaaaa cccggcaatc aaaatgccgt
      361 ctgaaaccgt gtttgatttt tgacgacatt ttcctcattg gaggggcggc aattatttat
      421 ggaaaataga tttaaaaaaa tcgttaagga tattcttagt tttgcccaat ttgtttccga
      481 agcggaaaat aaaattccta accaatcctc tcgttattac gattgctggt gggaattgga
      541 aatcttgaat gcttccgcat tggaagattg ggaaatatcg ggaaagcctg aaaaatggga
      601 gaaatggaca gaaaaatatc agaccgaagc gttacagtca gtcaagcatc tatttgccga
      661 agaagttcct gaaaataata tagtttgcga tatttctcat tttttgcatt ttgccgattg
      721 ggcagaagcc gacattccca atcaatcacg tttatatgcc gaatattggt gggaaatgga
      781 aatgttcaaa atgtcggttt tagaagattg ggaacgctcg ggaaaaccgt tagattggaa
      841 aaaatggcaa aggcaatatc gatctgaagc atttgaatta gttaaaaaat tgaaccgaag
      901 caaacaagag taacaagccg cagcctgcat aagggttttt aaagtacggc agcaaccgta
      961 gattaaatta aaaatcaaac aatcaaagct cgtctgatat aatcgatata ttctgttaaa
     1021 cgaaaaataa atatgtttaa aaaattcaaa ccggtactgt tgtcattttt tgcacttgta
     1081 tttgcctttt ggctgggaac gggtattgcc tatgagatta atccgcgttg gtttttgagc
     1141 gatacggcaa ctgaagtacc tgaaaatccg aatgcttttg tggcgaaact tgcccgcctg
     1201 ttccgaaatg ccgacagggc ggttgtcatc gtgaaggaat cgatgaggac ggaggaaagt
     1261 cttgccgaaa ctgtggatga cggtccgttg cagtcggaga aggattatct cgcgctcgct
     1321 atccggctca gtcgtttgaa agaaaaggcg aaatggtttc acgtaacgga gcaggaacat
     1381 ggggaagagg tttggctgga ttactatatc ggcgagggcg gtttggttgc ggtttcgctt
     1441 ttgcaacgct cgccggaagc gtttgttaat gccgaatatc tgtatcggaa cgatcgtccg
     1501 ttttctgtaa atgtgtacgg cggaacggct cacagggaaa attatgaaac gacaggagaa
     1561 tatcgggttg tttggcaacc ggacggttcg gtatttgatg cggcagggcg cgggaaaatc
     1621 ggggaagatg tttatgagca ttgcctcggg tgttatcaga tggcccaggt atatttggcg
     1681 aaataccggg atgtcgcgaa tgatgagcag aaggtttggg acttccgcga agagagcaac
     1741 cggattgcat cggactcgcg cgattatgtg ttttatcaga atatgcggga attgatgccc
     1801 cgggggatga aggcaaacag tcttgtggtc ggctatgatg cggatggtct gccgcagaaa
     1861 gtctattgga gtttcgacaa tggaaaaaaa cgccagagtt tcgaatatta tttgaaaaac
     1921 ggaaatcttt ttattgcaca atcttcgacg gtagcattga aagcggatgg cgtaacggcg
     1981 gatatacaga cctatcatgc gcaacagacg tggtatttgg atggcgggcg gattatccgc
     2041 gaagagaaac agggagacag actgcctgat tttcctttga acttggaaaa tttggaaaaa
     2101 gaggtgcgcc gttatgcaga ggctgcggcg agacgttcgg gcggcaggcg cgacctttct
     2161 cactgaacgc aaatgccgtc tgaaaccggt tttcagacgg cattggagag tcaaacgctt
     2221 tttgcaatgc cgtatatgcc gaatccgatt cgctacaaat tgaaaacaac aatccaaaaa
     2281 acggaaatac acattaaacg ggtcttttta ccgaccgtat catcccatag gttttccaca
     2341 ccttttttca tccataaatt caaaccattt aagtttgcat ccttaccgac aattgcagca
     2401 gcatcatccg cataacggtc acccaaatta gccaaataac gccaaccgcc ataggtatcg
     2461 ccacggaaag cataaccacg caaggtttgg atttgttctg gtgttaaaac aacttcaccc
     2521 tcattcataa taagatcctt taaattattt tttacttgaa ttgtttacaa aactctgtac
     2581 caattgctct acttgttggt gataacctac caactcctgc tgtgtgagct gctgcttttc
     2641 ccgaataaac gggtaatacc gtaccgtgaa accgttaata caggaaccta cgctagtgct
     2701 gtaatcgttc tctccatacc tatgatagtt ccgatgagta ataatcgccg gattttctcc
     2761 ttttttaata tagtaaaatt catgaaaata tggattttca ggaatatgtc ttttatcttc
     2821 ataagcctcg tattcaggat aggcagatgg cattttttta accccgtaag taagcaatct
     2881 ttctcccata gtcgtgctta aactacacgc atcttttcgg ataacaatat cgtccacgct
     2941 atccaaaccg tcgcgtagaa attggataaa gtgcgctttg ttttgctggt tgtatggctc
     3001 aagaacccag ccttggtcgg tttttttgta atagacgcta atctctatca aattaatgga
     3061 gtaagcatcc tgcccgggcg tgtcggaaag ccattttgtg gcattgtctt gcgtataccg
     3121 tgcaagatag ccataaggga taaccatttt gatgccctga aaatcaaatg taaccgtatg
     3181 ttcaaatcct gtcattggct cgagattgtt gaaactggtt tgttcattaa caggttcaca
     3241 tgaggtcata gtaaaaacac tcccaataaa caaaataaaa agttgataaa tggtagtagc
     3301 atatggtctc ataatttcaa gcttagaaat tagttaaaga atatattcat ggtagatact
     3361 aactctttaa gttgacatat tgtgcgatgt cgagggctgt accgtatttt cccatcgtat
     3421 tttcccatcg tattttccca tcgtattttc ccgttcttta tatgagcggc aggcatcctc
     3481 ataaaagaag ggataccgat aaggaaaaat accccagcat aaaaaatata ctgattaatc
     3541 aatacaaatc caacaaacag ttcatcgtat tcttttctgt tggcgtataa aaatactgat
     3601 atattccttc actttcatag ggcgacgctt tatgtaccgt cctgtgtgtt gaaacgtcgg
     3661 ttttccttac cgcctgcccc gcttttccct gattgctgcc aactgccgtc cgaacttgaa
     3721 ccgtcaggtt tcagacggca ttttttctgc ctgctcccac aattgccgtt tttgtgcggc
     3781 ggtcagtttt ttgacggcga tttcctgcct gagtgccgtg cctttatcca tcccgcctgc
     3841 aacgatacgc atcgccaccg gtttgaatac gcgggtatat ttcgcgcctt taccggttgt
     3901 gtgggcggca agccgctgtt gcggattcgg gctgatgccg caatagaacg cgctgttttc
     3961 acacaatatc aggtaaacgc tccagtttga cgcgttcatc tgcctgcatc ctgagttttc
     4021 tccccgttcc aaggcgcaac tttcagcagc agcagcatac ccggtagggc gagtgcgaaa
     4081 cacagttgga aaaacggtac ataaccgagc cattcaatca gataaccggc aaaggaattg
     4141 atgaccgtgc gcgggacggc ggacaggctg gtaaacagcg caagctgcgt tgcggtaaat
     4201 gcgggattgg tttcacgcgc catatacgat acgaacgccg ccgtccccaa ccccacgccg
     4261 accgcttccg cgccgataac tgccgccagc atcagcctct cgcctgtgcc gaccgtgtcg
     4321 aaaggtccga accctgccag ccatacaaac cccaaaacgg ttacagcctg caccgcgcca
     4381 aatagccaca aggctttgtt tacgccgatt ttcagcatcc acacgccgcc caagatacct
     4441 gccgccactg ccggccacag tcctgcattt ttcgcaatca aaccgatgtc tgtcttgctg
     4501 aaacccatat ccagataaaa cggcgttgcc aacgcggttg ccatactgtc gccgagtttg
     4561 taaaggaaga taaacagcag cacgcacacc gccgaagcga tgcccttgcg cgtaaaaaat
     4621 tctttaaacg gctctaccac ggtctgcttc aacgttttag gaacggcagg cggcaacacg
     4681 ggttcgcgcg caagaaacag cgtcatcaga aggccgggca gcataaataa tgaagtgata
     4741 acaaatactt ctgaccacgg catcctgtct gccaacacca aactcaatga accgggaatc
     4801 agggcggcaa cccgataggc gttcacatga accgagttgc ccaaacccaa ttcttcgtcc
     4861 gacaaaatct cgcgcctgaa cgcatccaat acaatgtcct gactggcgga aaaaaaagcg
     4921 acaagcaccg acaagccggc aatcagcggc agatgattac gggggtttaa aaaggcatag
     4981 gccgccaaag ccgccagcaa ccctgcctgc gtcagcagca tccacccgcg ccgccgtccc
     5041 aaaacgggca gcctgaccgc gtccatcagc ggcgaccaca aaaatttcca agtaaacggc
     5101 agaccgatta acgccatcag cccgatgctc ttcaaatcca cctgctcgct gcgcaaccac
     5161 gccggaatca ggttaatcag aaagtacagc ggcagccccg aggtaaaccc cgtaaaaata
     5221 caaacaagca tatttcggga aaagatttgc ccgataaaac ctgatttcga tacagtcatg
     5281 gttcaaacag ccaagaaata aaagcctatt gtagcaaata tcgcccaacc gctgaaaaac
     5341 accgcccctg ctccgccgtt ttgccgttca aatgcaaaat gccgtctgaa actgtgttca
     5401 gacggcattg cttttctgaa aacccgacgt gcttacaggc tgtaatacat ttcaaattcc
     5461 agcggatgcg gtgccatgcg gatgcggcgg acatcttcct ctttaaaggc gatatagctg
     5521 tcgatccagt ctttgctgaa cacgccgccg cgcaggagga attcgtggtc ggctttgagg
     5581 gcggcgaggg cttcttccaa agaggcgcaa acggtcggga cgagcgcgtc ttcttccggc
     5641 ggcaggtcgt acaggttttt atcggcagga tcgcccggat ggattttgtt ttgaataccg
     5701 tccaaacccg ccatcaacag ggcggcaaat gccaaatacg ggttggcggt cggatcgggg
     5761 aaacgcgctt cgatgcggcg cgccttgctg ctgttcacgg acggaatgcg gatggaagcg
     5821 gaacggtttt tggcggaata tgccagtttg gtcggtgctt caaagtgcgg cacgaggcgt
     5881 ttgtaggagt tggtggacgg attggtaatt gcgttcaggg ctttggcgtg tttgatgatg
     5941 ccgccgatgt agtagagcgc ggtgtcgctc aagccggcat agccgtcgcc tgcgaacagg
     6001 ttttgaccgt ctttccaaat ggattggtga acgtgcatac cgctgccgtt gtcgcccata
     6061 atcggtttgg gcataaaggt ggctgttttg ccgaagttgt gggcaacgtt ttgaatcaca
     6121 tatttcatgt cttgggtttg gtcggcgcgt ttgaccaagg tggcgaaacg cgtgccgatt
     6181 tccatttggc tgccggtacc gacttcactg tggtggactt cgacttcgat gccgagtcct
     6241 tccaaaatgt ttaccatcgc ggaacgcaaa tcctgaccgc agtcaatcgg cgcgacgggc
     6301 gcgtagccgc ctttgacggc gggacggtgg ccggtgtttt gaccgtccat atgcaggccg
     6361 cttgcccacg cgccgctttc ggacgtgatt tcgtaacggg ttttgtgcat gtcggtttca
     6421 aattctacgc cgtcgaagac gaagaattcg ggttcggggc cgaagtatgc cgtgtcgccg
     6481 atgccggaag atttcaaata ggcttcggcg cggcgggcga tggagcgcgg gtcgcggtcg
     6541 taaccctgac cgtcggcagg gtcgatgacg tcgcaggtaa tgacgacggt aacatcatca
     6601 taaaaaggat cgacgaaggc tgtagacgca tcgggacgca actgcatatc ggaagcctga
     6661 atgcctttcc agccgccgat agacgaaccg tcaaacgcct gaccgttttc gaaccactct
     6721 tcggggtctt ccaacacgat gcgcgcaggc acggtaaagt ggtgctgctt gcctttggta
     6781 tcggtaaagc gcaaatcgac aaagcgggct tcgctttctt caatcaattt tacggcgttt
     6841 ttaatagaca tttttcagct cctggaaaag ggaaatagca aaatatgcgg aacgcggcag
     6901 attagctcaa agccagcatg attctaccac gtccatgccg tctgaaactg gccctgcgtc
     6961 aaattccaag ctgtaaaata tagtttcaga cggcaatatt gtcaacaata tgcttcggtt
     7021 tgcccgttcc caagcagaat gcccgccctt ttatccgaat gccgacaaaa cagatgccgt
     7081 ctgaaagcag ttcagacggc atctgttgtt tggtttgaaa acggtcagcg aggatagccg
     7141 ttgcagacaa atttgtactg agcctctcca agcgtaccgg gaataacata attccattcg
     7201 gcataggatg gtgtataaga agcgatagag tttcccgatt tggaatacca cgttgccgtc
     7261 aatatcctaa aacgtttacc actgcaatcc atttcttcca acgttttagc tgtattaaaa
     7321 aattttccat tgccatactt ttgcggcttg gcgaaccttg tttcaaacca tgcactgttg
     7381 tccgtacggc tgtctgcatc accaaacgtg gcagtattac tatctaccaa tacccaatcc
     7441 gccgccaccg ccggtgttgc caaccccaat gcaaatactg ccaagcacaa catctttttc
     7501 atgtccgacc ctctcgtcct tgtcaacaaa ataaagttaa attgtaaaaa aaaacgactt
     7561 ttttcaagag aaaaatattc cgtcatgcta tctgacaacc gtttcagacg gcatcgtttc
     7621 catctgtacg ccgccgttat aaaatagcct tcccgtcttt tggaacacga ccatgaccgc
     7681 cctcccccgt tactccgttt ttggcaaccc cgttgcacac agtaaatcgc cgcaaatcca
     7741 tcagcaattt gcccttcagg aaggcgttga cattgaatac gaacgcattt gcgccgacat
     7801 cggcggtttt gcacaggcgg tttcgacatt ttttgaaaca ggcggttgcg gggcaaacgt
     7861 taccgtaccg ttcaaacagg aagcgtttca tctggcggac gagcattccg aacgcgcatt
     7921 ggcggcaggt gcggtcaata cgctgattcc gttgaaaaac ggcaagctgc gtggcgacaa
     7981 caccgacggt atcggtttga ccaacgacat cacgcaggtc aaaaatattg ccatcgaggg
     8041 caaaaccatt ttgcttttgg gcgcaggcgg cgcggtgcgc ggcgtgattc cggttttgaa
     8101 agaacaccgc cctgcccgta tcgtcattgc caaccgtacc cgcgccaaag ccgaggaatt
     8161 ggcgcagctt ttcggcattg aagccgtccc gatggcggat gtgaacggcg gttttgatat
     8221 catcatcaac ggcacgtccg gcggtctaaa cggtcagatt cccgatattc cgcccgatat
     8281 ttttcaaaac tgcgcgcttg cctacgatat ggtgtacggc tgcgcggcaa aaccgttttt
     8341 agattttgca cgacaatcgg gtgcgaaaaa aactgccgac ggactgggta tgctagtcgg
     8401 tcaagcggcg gcttcctacg ccctctggcg cggatttacg cccgatatcc gccccgttat
     8461 cgaatacatg aaagccatgt aatatgttcc gcatcgtcaa atggctgatt gccctgcccg
     8521 tcggcatctt tatctttttc aatgcctatg tgtacggcaa catcattacc taccgcgccg
     8581 tcgcgcccca tcggactgcc tttatgtcga tgcggatgaa gcagtttgaa caggaaggtc
     8641 gcgatgtcgc actggattac cgctgggtgc cctacaaccg catctcggtc aacctgaaaa
     8701 aagccctgat tgcttccgaa gatgcccatt ttgccgggca cggcggcttc gattggggcg
     8761 gcattcaaaa cgccatcagg cgcaaccgga acagcggcaa agtgaaggcg ggcggctcga
     8821 ccatcagcca gcagcttgcc aaaaacctgt ttttaaacga aagccgcagc tatatccgca
     8881 aaggcgaaga agcggcgatt accgcgatga tggaagccgt taccgacaaa gacaggattt
     8941 ttgaactgta tttaaactca atcgaatggc actacggcgt tttcggcgcg gaagccgcgt
     9001 cccggtattt ttatcaaata cccgccgcca agctgaccaa acagcaggcg gcgaaactga
     9061 ctgcccgcgt ccccgccccg ctctactacg ccgaccatcc gaaaagcaaa cggctccgca
     9121 acaaaaccaa tatcgtgctc agacgcatgg gttcggcaga gttgcctgaa agcgacacgg
     9181 actgagtgcc gccgcaatat aaaatgccgt ctgaaatatc attcagacgg catatgcttt
     9241 ctgcctttac ccttttctct aaaacgcaac ggggcggtca ttatcgcttt tgtccttcta
     9301 cataacggtc agacacaaaa cgtgtccgac acggacaact tcccaaaaac ggcaggcaaa
     9361 aaaaataacc gcttataagc ggttttttgg tggccagagg cggaatcgaa ccgccgacac
     9421 acggattttc aatccgttgc tctaccaact gagctatctg gcctttgtct gtctcgttaa
     9481 gagatgcgta ttaaaccaaa ttacgggacg acatgcaagc atatcgggca atattctttg
     9541 aaaacaggtt taagtcaatg ttttaaattt gaattaattt atgtaaaaac aacataaatc
     9601 cgtatttttg ccgcctgccc gacgatatta ggtcgcccgt ctgaaaaata ttttcaatat
     9661 ttgaagttct tacccagata aacagaacga acctgttcgt ttccgaccaa atcatcaggt
     9721 tttcccgatg ccaacaccgt gccgtctgaa ataatgtagg cccgatcgca gatgctgagg
     9781 gtttcgcgta cgttgtggtc ggtaattaat acgccgatac cgcgcgactt gagaaatccg
     9841 atgatttttt ggatgtcgat gacggcgatg ggatcgacgc cggcaaaagg ctcgtccaac
     9901 aaaataaaat gcggtttcat ggcaagtacg cgggcgattt cgacgcgtcg gcgttcgccg
     9961 cccgacaaag atggggcagg attattgcgc aaacgctcga tattcaggtc ggcaagcagt
    10021 ttttcaactt cgccgtcgat gcggcttttg tctttggtac tgatttctaa aatggcgcgg
    10081 atgttttgtt cgacggtcat tttacggaaa atagaggctt cctgcggtag gtagccgaca
    10141 cccaggcggg cgcgttcgtg tatgggcagg tgacgcaatt cctgcccgtc cagcatcacg
    10201 ctgcccgcat cggcggcaat gagtccaaca atcatgtaga agctggtggt tttacccgca
    10261 ccgttgggcc cgagcaggcc gacgacttcc ccgctttcga tttcaaggga gaagctttta
    10321 acgacttggc gttttttgaa acttttttgc aggttttgaa caacaaggcg gctgacgttt
    10381 gcgctcataa atatctcttc aatatcttcg gtcggcaaat gccgtccgaa ccaggtttat
    10441 gtttcagacg gcattttggg attattcgga tttttgcgta ctcgaaggct ggataacgac
    10501 gctgaccctg ccggatttgg aagcggattt tgcgccggat tttgtgctgc cgctgatggt
    10561 atagacttcg gttttggtgt tgtatgtaat caccgcacct tcggcgacat cgccgccgcg
    10621 ctgtactttg gcattaccgg ttaagactac ggtgctgcct gcagatgaat aagcaacgtt
    10681 gtttgcctgt ccgcgcaccg tgcctttgcc gccgtccaat gtctggctga agcggactgg
    10741 cgaaccttcc gccctcacgg attcgccgcc tttgccgccg cgtgtaacat tgacgcgggc
    10801 ggcggaaata ttgagcgtac cctgtctgat gacgacgttt ccgctgaatg tggtgctttg
    10861 gttggcttga tcgagcgaac cttggtcggc ctcaatctga ataggctgcc tgctgtcgct
    10921 ttgaagggca aaagcggggg acgccgaaaa aaatgctatt aaaacaaata gcttacatat
    10981 cttttgtatc ataaatcgtg gctttcactt tagatgagaa gttcaacatg cctgttttgt
    11041 ggtcgtaagt catgccgccc gcctgaccgt gcgatgcacc atattggaaa ctgacaggcg
    11101 tatcggtttg ggcatattga gattcggtat cgacgtgcag cttttcggct tcaactttac
    11161 ccgcctgccg tttgccgtca gcggttttgg tcagcacaac gttgttttta aaaagaacct
    11221 gtttgttttc ggtatggtaa acggcttcat cgctgccgac ttcgtacaac aacctgcctt
    11281 cttggaagaa cacgagatgc ggcgaatcaa aatggatgtc gctgctttcg ggaaactgtt
    11341 tcgcgccttt cgcgctcaaa tgttctttta agtatccctg ttcgtcaaac cgcctgccat
    11401 ccaagccgtc cattgtgtat tgcggttcgt cgggattgag cctgacttct tcaatctcga
    11461 cttcgctgat gcgtcccaac catgccgaca gaccgcccaa ggcaaccgcc aatatcaatg
    11521 ggaacgcaat tccgtaccgc catcttattt tcatttgatg tactcgttca aagccgcgcc
    11581 caaagtccct tgcgcctgca taatcaggtc gcacacttcg cgcaccgcgc ccgcaccgcc
    11641 ggggcgtttg gcaatatagg cggcgtgttg cagcgtaaac caatgcgcgt cgggaacggc
    11701 aaccggcaat ccgcaacgca ccatcatcgg caaatcgacc acgtcgtcgc cgacaaaggc
    11761 acactcggct tcttccacgc cgacctgagc gcgcagctct tcatacgcgg cgcgtttgtc
    11821 cgaaatgcct ttgaagtagt aatttatgcc caactgtttg acgcggatgc cgacggaggg
    11881 cgcgtcccga cctgtgataa tcgcagtctg cacgccgctt gcctgaagca ttttcagacc
    11941 gtgtccgtcc agtgtgtgaa acgatttgat ttcttcgccg ttatcgcgga taaagatgcg
    12001 cccgtcggtc aaaacgccgt ccacatccag gatcaacagt ttgattttgg cggcgcgcgc
    12061 ctgtaattcg ggagaaattg cctgcatggg atttccttgt tttcagacgg catatcttac
    12121 cacaaatgcc gtctgaaacg gctgcaacgc cttatacaat ccgtgccgcc agcaggtcgt
    12181 gcatattcag cgcgccgatc agcacgccat ctgcatcggt aaccagaagc ccgttcacat
    12241 ggtttgcctg catgactttc agggcttcgg tggcgagacg ttcggcggag atggttttag
    12301 gatgcgtatg catgacttcg tctatcgaaa gaccggtaaa attgtcgcat tcttgaaaca
    12361 ggcggcgcaa atcgccgtcg gtgaatacgc ctttcagacg gccttgcccg tccgttaccg
    12421 ccaacatgcc cagccctttt tcactcatgc tgacgatggc ttctttcaag ggcgtgccga
    12481 gtcggacggc aggcaggccg ccgcctttgt gcataatgtc ggcaacgcgc aaaagtaggc
    12541 gtttgccgag gctgccggca ggatggctca aggcgaaatc gtcgggcgtg aacgcgcgtg
    12601 cgcgcagcag gacgaccgcc aacgcatcgc ccaaagccat gacggcggtg gtgctggtgg
    12661 tcggggcaag ccccagcggg caggcttctt tggaaaccga cgccgtgatg tggatgtcgg
    12721 catggcgcgc catggttgaa tcggggcggg cggtgatgca gacaagcgtg atgtctttgc
    12781 gtttgagcgc ggggatgatg gcggcgattt cgtcgctttc gccggaattg gaaatcgcga
    12841 cgaccacgtc gttgtccaca atcatgccca aatcgccgtg tgccgcttcc gcagggtgga
    12901 caaaaaacgc gggcgtgccg gtcgaggcca tcgttgccgc cattttgcgc ccgatatgtc
    12961 ccgacttgcc catgcccgtg ataacgaccc tgcccttgca gtgcaacaac gcgtctgccg
    13021 caaggacgaa gttttcgtcc aattccgttg caatttcgcg caagccttcc tcttcggtgt
    13081 gcaacacttc gcgcgcccag tcgagatatt ttccgttttc tgccattgcc atccgccttt
    13141 gctgtaataa aactacccaa aatttccgaa tcggggtagc ataagccttt gaaacataca
    13201 catccatcca aaggaatcag aaatgactat tttatcggac gttaaagcat taggacaaca
    13261 aatctggctg gacaaccttt cccgctcgct cgtgcaaagc ggcgaattgg cgcaaatgtt
    13321 gaaacaaggc gtgtgcggcg taacttccaa tcccgccatt ttccaaaaag ccttcgccgg
    13381 cgatgccctg tacgccgatg aagtcgccgc gctcaaacag caagatctga ccccgaaaca
    13441 acgctacgaa accatggcgg ttgccgatgt gcaagccgcc tgtgacgtgt gccttgccga
    13501 acacgaatcc accggcggca aaaccggttt cgtcagcctc gaagtttcgc ccgaattgtc
    13561 caaagacgcg caaggcacgg tagaagaagc gcgccgcctc tacgccgcca tcgggtgcaa
    13621 aaacgcgatg attaaagtgc ctgccaccga cgcaggcatc gatgcgctcg aaaccctcgt
    13681 ttcagacggc atcagcgtaa acctgaccct gctgttctca cgcgtccaaa ccctcaaagc
    13741 ctacgccgcc tacgcgcgcg gcattgccaa acgcttggca gccggacaaa gcgttgccca
    13801 tatccacgtc gtcgccagct tcttcatctc ccgtgtggac ggcgcgctgg acacaacact
    13861 gcccgaccac ctcaaaggca aaatcgccat cgcccttgcc aaagccgcct accaagactg
    13921 ggcacaatac ttcggcagcc cggaatttgc cgcactggaa acccaaggcg caaaccgcgt
    13981 gcagctttta tgggcatcta ccggcgtgaa aaacccggcc tatcccgaca cactctacgt
    14041 tgacagcctg atcggcgcgc acaccgtcaa caccgttccc gatgccacgc tcaaagcctt
    14101 catcgaccac ggcacagcca aagctacgct gaccgaaggc gcggacgaag cacaagctca
    14161 gctcgccgaa accgccgcgc tcggcatcga tgtcgaaacc ttggcggcac gtttgcagga
    14221 agacggtttg aaacagtttg aagaagcgtt tgaaaaactg ctcgcaccgt tggtttaaac
    14281 ttttcatccc cgatgccgtc tgaaaggctt tcagacggca tcgcccgttt gaccggacac
    14341 agtgcggcat atcaaggatt caccctaaaa atattaatgg gcaagaagtt aatgcacgac
    14401 attacccacc aacacaaaag gaaatagaat catggcaaaa gcactcgaaa tcatttcacc
    14461 caatgaaatt tattcagatc tgatttttaa ggatccggta cctccccata caatatatac
    14521 agaattgatg aaactggtta gtagaaatgc aagtaatgag agaatcgcag atagagcctt
    14581 cgattttttc tcgtccggcc ttatggatgg ttcagctacc gaagagcaat ataatgccct
    14641 atatgattta accttacttg aagaacccgg gatggaactg aataaagatg agataatggc
    14701 tttgattaat agtcttaaat aaaggggccg taccggatat gtacacagga cgtttcgccc
    14761 ccagcccgac cgggctgctc cacatcggct cgctgctgac cgccgccgct tcctatgccg
    14821 atgcacgctc aaacggcggc aaatggctgg tccgcatgga agacctcgat ccgccgcgcg
    14881 aaatgccggg ggcggcaagc catatcctgc acacgcttga ggcattcgga ttcgagtggg
    14941 acggagaagt cgcctatcag agccgccgtt acgccctgta tgaagaaaca ctatgccgtc
    15001 tgcaaaccgc cggactggtc tatccctgcc attgcagccg caaagactgg caggctgcgg
    15061 cagtccacgg cgcagacgga ttcgtctata acggacgttg ccgcaatccg cagcaacgcc
    15121 ctgcactgca aggcaaacag cctgcgtggc gcatccgcgt ccccgaccgc gacatcggct
    15181 tttcagacgg catcgtcggc ggttacgccc aaaacctcgc ccgcgacatc ggcgatttcg
    15241 tcctgcttcg tgcagacggt tactgggcat accagctcgc cgtcgttgcc gacgatgccg
    15301 aacagggcgt tacccacatc gtccgcggac aagacctgct cgtttccacg ccgcgccaaa
    15361 tctatttgca gcagtgtttg ggcgttccga caccgcaata tgcccacctg ccgctgctga
    15421 ccaacgcaca agggcagaag tggtcgaaac agacgctcgc ccccgcattg gatttaaacc
    15481 gccgcgaaca actcctccgc caagtgttcc gttacctcaa gctgcccgaa gcaccggaaa
    15541 ccgaccgccc tgccgaactg ctcgactggg cggtggcaca ctgggatatg ggcaaagtgc
    15601 cgaaacacgc cattaccacc ccctaaacac cgcccgacaa acgcacaaat gccgtctgaa
    15661 gccttcagac ggcatttcac acatacagcc ccgccctatt cgcgcgtatt tgcccgctca
    15721 acctctttcc acgcttcgag aatcaggctg ccgtcgttgt ctttcaagag cgttgcgtcg
    15781 gaaagcatac gccgccaagt ccgcgcacct ttcaaaccgt gcatcagccc gaggctgtgg
    15841 cggacgatgt ggcgcaagat tgtgccgcgt ccggcttgga tctgggcttg gctgtatgtg
    15901 tagaggcact gcaccaaatc ggcgtattcg atcgggctgc gagtatcgcc gtaaaacaat
    15961 ctgtcccatt cgcgcatcac catcgggttg tggtacgcct cgcgcccgac catcacgccg
    16021 tcaacgtgtt gcaggtgtcc ggcgattgct tcgttggtgg tgatgccgcc gttgatgatg
    16081 atttccagcc cgggaaactc ctgcttgagg cggtaaacgt aatcgtattt caacggggga
    16141 acgtcgcggt tttctttggg ggaaagaccg tccagccaag cgttgcgggc gtggacgatg
    16201 aaggttttgc aggcggtttt gtcgcgcagc gtgccgacga aatcggcaac ggtttggtat
    16261 tcggtctgcc tgtccacacc gatacggtgt ttgacggtaa cgggaatacc gaccgcgtcc
    16321 tgcatggcgt tgaggcagtc ggcaaccagc ccgacttcgt tcatcagaca cgcgccgaac
    16381 gagcctttct gcacgcgcgg actggggcag ccgcagttga ggttgacctc gttgtaaccg
    16441 tatgcctcgg cggctttggc ggctttcgcc aaatcggacg gatcgctgcc gcccagttgc
    16501 agggcgacgg gctgctcgcc ttcgttgaac atcaaaaagc ggtctttgtc gccgtaaaca
    16561 atcgcgccgg cgttgaccat ttcgctgtac agccaagtat tgcgggtaat ctggcgcgcc
    16621 atataacggt agtgcctgtc cgtccagtcc aacatcgggg cgacggacag gtggcgcggg
    16681 ggaagggtgt gtgtattgtc gttcattgtc gatattttat cattgcggat tcaatttcaa
    16741 tccggaacaa ccttcatttt tggggaaatc cgcgtcatga aagagtccag cagtatggtt
    16801 accggcccgt cgttgcagag cgacacctgc atatgcgtgc ggaaacgccc tgtttcgaca
    16861 tgaatcccgt gtccgcgcaa cagttccgcc gttcgcaggt aaagctgctg cgcctgttct
    16921 gcaggtgcgg cttgggaaaa cgaaggccgc cgcccgcttg ccgcatcggc ataaagtgta
    16981 aactgcgaca ccagcagcac cgcgccgccg acatctttca aagacaggtt caacttgccc
    17041 gcttcgtctt caaacacgcg caaatgggcg attttgtcgg cgatatagcg tgcatctttt
    17101 tctgtgtcgc tatgcgttac accgagtagc acgacgaatc cgccgtcgat tttgccgcag
    17161 gtttccgtgc cggcttcgga cacgacatcc acctttgcac ctaccgtttt ctgtatgacc
    17221 gcacgcataa gccgtcctcc ttacctgaac gtgcaatgcc gtctgaaaag gcttcagacg
    17281 gcatcggtat cggaaatgcc attacggttt gacaccgttt tcttccaaaa gggcatcgat
    17341 tttcaagcgg gctttttcct gtctcaaacc ctgctccaat tggtttctga ccaactcgaa
    17401 aggttgcgcg tcggggtttt tcccgacctc gctgagtttg aacagataat agcgttcgcc
    17461 caatttgacc ggattgcggg taacgtcgcc ccgattcatc gctgcaaact gcgaagccag
    17521 cggctcggga agctgctgcg ccataatgaa accgtcaaaa gcctgctcgt cgttcggata
    17581 acgcttcatc agcccttcaa aagacagccc tttgagcagg agctgctgcg cctgacgcgc
    17641 ctcctcttcg gttgcgaagc tgacctgctg caatttgatc atgcggattt gccgctcata
    17701 aaactgacgc agtgcgcttt cggaaaccgt ttccgaacgt tccagaaaac ggacgtactc
    17761 ctcggcataa aaagacgctt cggcgatttt aaagcggttt tggacatcct tatccttatc
    17821 caaaccttcc ttcaatgccc tgtttttcaa aacttccaaa gtttgcagcc ggcggacggc
    17881 atcgtttcgg attgcctgcc cgtccggttt ttgggactgc tccgcatgcc ggtctgcctg
    17941 ctgcatgatc tgcgccacca gcgtatccac caaagccggg tcgatttcgg gtgctttggc
    18001 tgccgcaaaa cctgccaaca ttgcagcaat aactgcggca gcggtttttt tctgtttcat
    18061 ggtattccct gtcctgcgcg gcggcaaaac gcctgtctga acgtcttgcc gccatatccc
    18121 gttttcagaa ttattttgca ggtttgatgt ttgccttgcc caacagtgca ccgacggcac
    18181 ggtcaatccg ttccgcctga aggttgcccg caatctgtcc tttcatttca tcaaaagaag
    18241 gcacttttac ctcgcggctg tcgttgacat aataaacgcc gtagaaatcg ccgtttttca
    18301 gcggcgttgc cgtaaattcg ccttttttca agtccttaat tgcctgataa agcggcggaa
    18361 caccctgttc caaatctttc agcggcacat atccgaccgg cgcaccggtc tgtttggtac
    18421 ggtcgttgag ggaatattgt ttcaagacgg catcgaaacc ttttttcgcc ttcaagtcgg
    18481 caaccgcttt ttttgcattt tcttccttgt cggtcaggat ttcgcccaac tggacttcct
    18541 gcgtaccttt gtaaaaaccg ctgatattgt catatgcggc ttttacttcc tgctcggaaa
    18601 ccggttgggt tttggcgata tgcaatgcgt atgcctcgcc gttcaagcca tattttaccg
    18661 cctgccaaac ggttttgaag gacggtttct tgtcgtcgcc cgactttttc gcttcggcac
    18721 gcaatttggc aagcgcattt ttaaactctg ccgaccggtc gagtttcagg cgtttcactt
    18781 cctgtgcgac cacggtgttg accacttcgt tttccagcag ggattggcgc agttgcggcg
    18841 tgtcttcggc acggctgttt tccgcacgga atgcggcaac ctgcgcatca atgacggaac
    18901 tgtcgatttt ctgaccgttg acggttgcca gcgtttgggc aaacaggctg ccggaacagg
    18961 caagcagtgc aacggaagtc aggatttttg ctttcataat cttctctctt taaattttaa
    19021 attgaaatgt tcgggcagac ttcattcagt ccgccgtatg gaaatactcg tcaggggtag
    19081 ccgcccggat gccgagcgcg tgaatcatgc cgcctgaaaa caaatctttg agcagcgatt
    19141 tgaccgtttt ctggcggttc aggcggctta cgccttcaaa acggccgctg acgaccaaaa
    19201 cggcataatg tccgccgccg gtattgcccg cgtgtccttt gtgcagatgg ctctcatcgc
    19261 cgatttccaa caccagcgga tcgagcgtct gcaggcgttc gcggatcaaa tcgacggccg
    19321 gcattatttg aacaccgctt taaacggctt gatcagcact tcgctgtaca cgcctgcatg
    19381 aacataggga tcgtcttccg cccacgcctc tgccgcatcc aaagactcga actgcgccac
    19441 aatcaaactg cccgaaacgc ggttggagtc ctccggcaaa ggattcgggc ctgccgtcaa
    19501 cagccggcct tccgacttca gcgtctccag ccgtttgagg tgttcgggac gtgccgccat
    19561 acgcgcttca tacacattct ccccgtctgt tgccagcaac ataaaatatt ccacagtcaa
    19621 tcctcctttt tcagacaggt actcagataa ataccctgaa taataaagaa aacaagcatc
    19681 agtgcagtcg agccgaacat tttatagttg acccattgcg actcgaaccg ggtaaacaca
    19741 aaccagttgg cgatacccat aaaaatcagg aaaccgaccc acatatacgt caatttcgcc
    19801 catacggcat ccggaagctg aatctccctg ccgatactcg ccttcaagcc gtttttaccg
    19861 gcgaggtggc tgccccacag gaacagcgca cccagccaaa acaaaacgct cggcttccac
    19921 ataatgaagc ggctgtcgcc caaaacaatg gttgcgccgc cgaatacgac aatcagcacc
    19981 agtccgaccc actgcatcgt atccagcttt ttatatttcc aatacagaaa agccgcctga
    20041 accacaccgg caaccaaggc aaccgccgta gcggcaatca tatttttggt aacggtataa
    20101 gtagcaaaaa acaagatgac agacaaaagg tcgctgacga atttcatatc caaatgagaa
    20161 agcaaatcaa ataaacgcta atcatacagg cagaaccctg tttttgcacc tgcaaaacca
    20221 acaaattttg ttttgattga tgcaaatatg cacagatgtt tttgaaaaaa gatggagata
    20281 tgtcataatt tgtaaaaacg gcgaatgatt gccgtttaaa atgtggcggc ggtcggtaca
    20341 ttcacatatt aaatgcccgc cggccgactg ccccgccgat gcccgatgat gacgaaattt
    20401 acagactgta cgcggtcaaa ccgtattcag ccgccaaccc acaggggata catcttgaaa
    20461 aacaacagac aaatcaaact gattgccgcc tccgtcgcag ttgccgcatc ctttcaggca
    20521 catgctggac tgggcggact gaatatccag tccaaccttg acgaaccctt ttccggcagc
    20581 attaccgtaa ccggcgaaga agccaaagcc ctgctaggcg gcggcagcgt taccgtttcc
    20641 gaaaaaggcc tgaccgccaa agtccacaag ttgggcgaca aagccgtcat tgccgtttct
    20701 tccgaacagg cagtccgcga tcccgtcctg gtattccgca tcggcgcagg cgcacaggta
    20761 cgcgaataca ccgccatcct cgatcctgtc ggctactcgc ccaaaaccaa atctgcactt
    20821 tcagacggca agacacaccg caaaaccgct ccgacagcag agtcccaaga aaatcaaaac
    20881 gccaaagccc tccgcaaaac cgataaaaaa gacagcgcga acgcagccgt caaaccggcg
    20941 tacaacggca aaacccatac cgtccgcaaa ggcgaaacgg tcaaacagat tgccgccgcc
    21001 atccgtccga aacacctgac gctcgaacag gttgccgatg cgctgctgaa ggcaaaccca
    21061 aatgtttccg cacacggcag actgcgtgcg ggcagcgtgc ttcacattcc gaatctgaac
    21121 aggatcaaag cggaacaacc caaaccgcaa acggcgaaac ccaaagccga aaccgcatcc
    21181 atgccgtccg aaccgtccaa acaggcaacg gtagagaaac cggttgaaaa acctgaagca
    21241 aaagttgccg cgcccgaagc aaaagcggaa aaaccggccg ttcgacccga acctgtaccc
    21301 gctgcaaata ctgccgcatc ggaaaccgct gccgaatccg ccccccaaga agccgccgct
    21361 tctgccatcg acacgccgac cgacgaaacc ggtaacgccg tttccgaacc tgtcgaacag
    21421 gtttctgccg aagaagaaac cgaaagcgga ctgtttgacg gtctgttcgg cggttcgtac
    21481 accttgctgc ttgccggcgg aggcgcggca ttaatcgccc tgctgctgct tttgcgcctt
    21541 gcccaatcca aacgcgcgcg ccgtaccgaa gaatccgtcc ctgaggaaga gcctgacctt
    21601 gacgacgcgg cagacgacgg catagaaatc acctttgccg aagtcgaaac tccggcaacg
    21661 cccgaacccg ctccgaaaaa cgatgtaaac gacacacttg ccttagatgg ggaatctgaa
    21721 gaagagttat cggcaaaaca aacgttcgat gtcgaaaccg atacgccttc caaccgcatc
    21781 gacttggatt tcgacagcct ggcagccgcg caaaacggca ttttatccgg cgcacttacg
    21841 caggatgaag aaacccaaaa acgcgcggat gccgattgga acgccatcga atccacagac
    21901 agcgtgtacg agcccgagac cttcaacccg tacaaccctg tcgaaatcgt catcgacacg
    21961 cccgaaccgg aatctgtcgc ccaaactgcc gaaaacaaac cggaaaccgt cgataccgat
    22021 ttctccgaca acctgccctc aaacaaccat atcggcacag aagaaacagc ttccgcaaaa
    22081 cctgcctcac cctccggact ggcaggcttc ctgaaggctt cctcgcccga aaccatcttg
    22141 gaaaaaacag ttgccgaagt ccaaacaccg gaagagttgc acgatttcct gaaagtgtac
    22201 gaaaccgatg ccgtcgcgga aactgcgcct gaaacgcccg atttcaacgc cgccgcagac
    22261 gatttgtccg cattacttca accttct
//
LOCUS       DAWEWP010000014        22266 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWEWP010000014 DAWEWP010000000
VERSION     DAWEWP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.014"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(120..1034)
                     /locus_tag="WGN53_000396"
     CDS             complement(120..1034)
                     /locus_tag="WGN53_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0139429.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WGN53_000397"
     CDS             1290..2759
                     /locus_tag="WGN53_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ0139430.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WGN53_000398"
     CDS             3011..3382
                     /locus_tag="WGN53_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139431.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WGN53_000399"
     CDS             3455..4864
                     /locus_tag="WGN53_000399"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ0139432.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWTEVAAGKGFDLQFALDKVGGKYGFTSSKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="WGN53_000400"
     CDS             4910..5704
                     /locus_tag="WGN53_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139433.1"
                     /translation="MTLPMQETRFSILLDELAAKQKAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAARIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="WGN53_000401"
     CDS             5892..6668
                     /locus_tag="WGN53_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0139434.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7336..9807)
                     /locus_tag="WGN53_000402"
     CDS             complement(7336..9807)
                     /locus_tag="WGN53_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ0139435.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNLRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVREAAVVKLQ
                     AGDVVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9804..10982)
                     /gene="hflX"
                     /locus_tag="WGN53_000403"
     CDS             complement(9804..10982)
                     /gene="hflX"
                     /locus_tag="WGN53_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ0139436.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIATVRISVAENTGIDALREAIAEYCATAPNTDETEMP
                     "
     gene            complement(10987..12267)
                     /locus_tag="WGN53_000404"
     CDS             complement(10987..12267)
                     /locus_tag="WGN53_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ0139437.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNSRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12420..13145)
                     /locus_tag="WGN53_000405"
     CDS             complement(12420..13145)
                     /locus_tag="WGN53_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ0139438.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13218..13895)
                     /gene="radC"
                     /locus_tag="WGN53_000406"
     CDS             complement(13218..13895)
                     /gene="radC"
                     /locus_tag="WGN53_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ0139439.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQSVRSF
                     RQLGLMP"
     gene            complement(14018..15367)
                     /gene="gshA"
                     /locus_tag="WGN53_000407"
     CDS             complement(14018..15367)
                     /gene="gshA"
                     /locus_tag="WGN53_000407"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ0139440.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNVYALGEILCSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNSAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15627..17036
                     /gene="leuC"
                     /locus_tag="WGN53_000408"
     CDS             15627..17036
                     /gene="leuC"
                     /locus_tag="WGN53_000408"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ0139441.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17133..17387
                     /locus_tag="WGN53_000409"
     CDS             17133..17387
                     /locus_tag="WGN53_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139442.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17449..18090
                     /gene="leuD"
                     /locus_tag="WGN53_000410"
     CDS             17449..18090
                     /gene="leuD"
                     /locus_tag="WGN53_000410"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ0139443.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18260..18632
                     /gene="dcm"
                     /locus_tag="WGN53_000411"
                     /pseudo
     CDS             18260..18632
                     /gene="dcm"
                     /locus_tag="WGN53_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            18685..19080
                     /locus_tag="WGN53_000412"
     CDS             18685..19080
                     /locus_tag="WGN53_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0139444.1"
                     /translation="METLEALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKSKPDLSF
                     ETSPSPKLKNILESGLPTESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSWDIEL
                     KGAVTEEEKQLLNILLKERRKKNGLQKSA"
     gene            19095..19529
                     /locus_tag="WGN53_000413"
     CDS             19095..19529
                     /locus_tag="WGN53_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0139445.1"
                     /translation="MPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            19545..19706
                     /locus_tag="WGN53_000414"
     CDS             19545..19706
                     /locus_tag="WGN53_000414"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0139446.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            19707..20276
                     /locus_tag="WGN53_000415"
     CDS             19707..20276
                     /locus_tag="WGN53_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0139447.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNTGPG
                     FDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKK
                     KFEEAYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20310..21380
                     /gene="leuB"
                     /locus_tag="WGN53_000416"
     CDS             20310..21380
                     /gene="leuB"
                     /locus_tag="WGN53_000416"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ0139448.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21543..22106
                     /locus_tag="WGN53_000417"
     CDS             21543..22106
                     /locus_tag="WGN53_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ0139449.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQRPMEKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5364 a         5852 c         5793 g         5257 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctagggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcag aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ctttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccgga ggcggtaaca cattcggcag cttagacggt ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggtcg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccgaacg gatgaccggc aacgatgtga tgcagatgct
     3301 ggctccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgggaaacc ttggacaaat ggcgcggttt
     3601 ggcttatccc gaattggcgt ttgaaattat gcgcctgttc gttaccgaca ttccggagga
     3661 cgatttgcgc gatattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg cgatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgtg cgcagatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggtatgtttg acgactgtca ggatattgtg aaggcggtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg attaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgccggcta tttcaaagcg acgcaaagca atgacgagca
     4201 agtcagcttt tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac atattgccaa
     4261 acagatgggc ttgcccatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cgtaccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccatct atggacattt ccaaagcgtc caatttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtggacggaa gtcgcggcgg gcaagggctt
     4501 tgacttgcag tttgccttag acaaagtcgg cggcaaatac ggctttacct ccagcaagtc
     4561 cacccacgcc gaccgcctcg ccaccatcaa acaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggtggtttgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtacaggggc actccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ccatactgct agacgaattg gcggcaaaac agaaggcagc
     4981 catcgccccc cacctgcttg ccgacgggag caaggtatgg atacgcaaag caggcaggca
     5041 caatgcgcga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgcgcggcga ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggcaatttgg aaggcatccc gctcgatacg caaatccgcc aagaagccga
     5281 agccggaaag gcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcgcg gaatatgatg tcggacggaa aaaacatcag
     5401 ctttctagat tttgaagacg acccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacatcc attcgaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtatt gtcggatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cacgctgggg
     5641 acgggacgca ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 ataagccgcc gcccgccccg cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta tcccgaatcg
     5821 ggcagacatt tccactctgc cgcacctccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgcgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaactctggt tgccggcgac ctgaatccgg gcaaacacct ttacttgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagaccccga gatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gcgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcccgagaa gaattcgaac accacggccg tctgaccgac ctgatggtaa
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa cccgcaagac gaccgcgcga
     6541 tgctgtgcgg cagccccgcg atgctgaaag atacctgcaa agttttggac gatttcggtc
     6601 tgaccgtctc tccgaaaacc ggcgtgcgcg gcgattacct gattgagcgc gcgtttgtgg
     6661 atcaataagc tgcttgaaaa tgccgtctga acggcatcag cgataaaaaa acccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg ggtaaaaatt agaaatgcac atttccgtca
     6781 ttccaacaca ggtgggaatc cggacttacc tgtttcatta tcgtttgaaa ataaaagaga
     6841 aaccaaacat atagattccc gcttgcgcgg gactgacgac ggtgttctgc gtagccaaag
     6901 cagaaaaaca tccttattgt catttcatca tgatataaca ataaggatgt taaaaattag
     6961 ttatcattat atgcatgata tataagaaaa attgtcccaa taagaaatgt tatgcagaaa
     7021 ataaaatgaa aaggatggtt ttcattgaaa atggttgttc tgccatcgtc atacacagaa
     7081 ccggtatttg gaaaaaaata atccatattg acatcattat tccatttatc gcataaatcc
     7141 actttttaat ttttttccac attaatataa tctcttattt aataaaatca aaataaaaat
     7201 caaatgccta acaattcgat ctcaaatttt gaacgccaac gtgtttgagc ggtaacaata
     7261 catactttaa gtttgcaatc cttcatcgtc ttatttttgt tttcatcaac tatacgtcta
     7321 aagcaacgcc gtccgtcatt gttcggacgg cattttttca gactgcattt tcccccgttt
     7381 gtgaaggcgc agggcgttgc ccaaaaccgc cagcgaactg aagctcatac ccagtgcggc
     7441 tatccacggt tggacatagc ccaaaacggc aagcggtacg gcaatgatat tgtacgcgcc
     7501 cgcccatatc aggttttgcc ggataatatg gcgggtgcgc cgcgcctgat cgagcaggtg
     7561 ggcgacggta cgcaaatctt cgttcaataa cacaatgtcc gcgccgtccc tcgcaatatc
     7621 cgtcccgccc gctgcggcgg cggatacgtc tgcctgcgcc aaaacgggcg cgtcgttgat
     7681 gccgtcgcct atcatcagca cttttttccc ttctttttgc aaggctttga cgtattccag
     7741 tttgtcctcg ggcatggctt gggcgcggta gtgcgcgaca cccagggcac gggcggtttc
     7801 ggcaacggcg gtttcgcggt cgccgctgag gatgtgcagg gtcaggtttt tgcctgccaa
     7861 ctgccgcacc gcctccgccg cgctgtcttt caaggggtcg gtcaggtaga acacggcttg
     7921 gaaaccgctt tgactgccga ggtaaaccgc gctgccgccg ccttctgttt gcggttcttt
     7981 acctgaaatt tcggcgacat aggatgccct gcccaatgcc caaacctgtg tttcgccgtt
     8041 gacggtcagt tgcgcgccca cgccttcgcc gatgcggttg aggcgttgtt taatagcgat
     8101 gtcggggacg ctgccgtctg aaatgcggca gttgaggatg gcgcgggcaa ggggatgttc
     8161 ggactgttgt tctaaagcct gcgccaccgc gagaacaaag gcttcgtctg tgcctctcaa
     8221 caatgagata cggcggacgg cgggtttgcc ttgggtcagc gtgccggttt tgtcgaagat
     8281 gatgtcggtg gtttgggcga gggtttcgat tgcctgcttt ccgccgatta aaataccttc
     8341 gcgcgccagc gtaccggtag aagctgccag cgcggtcggc gtggcaagcg ataaggcgca
     8401 ggggcaggta atgaccagca gggcgacggt aatccacaat gcggtgtgcg cgtcggcgta
     8461 cagcgtccag ccgatgaaga cggggacggc aagcaggagt tcgccgaata tgaaagacga
     8521 ggcgtattgt tccgccaact cggcagtgcg cggtttttgc gctaaggcgc ggtcgagcag
     8581 gcggacgatg tgcgacagtc gcgtgccgcc gccggtgcgg tcggtgcgta taatcagggg
     8641 gctttgcgtg ttgagtgtgc cggcggttac tttttcagac ggcattttgg cgacgggcag
     8701 gctctcgccg gtcagcatag attcgttgac ggcactgctt ccttccagca ccgtgccgtc
     8761 aacggggatg gtttcgcccg gtttgaccag taccacatcg cctgcttgga gcttgacgac
     8821 ggcggcttcg cggacatcct gtacggcagg atagccgggc atccggtggc agaaggcggg
     8881 tatcagtttc accagccttt cggcagcatc gcctgccttg cgccgggcaa tgtgttccat
     8941 aaagcgtccg cccagcagga aaaacagcag catggcgatg gattcgaaat acatcccctg
     9001 ccccgcattg gtcgcaaggc tgtaaacgcc ggcgataaag gtcatgatga tggcgacggc
     9061 aatcggcgta tccatgccgg cgcggaggtt tttcaggtcg cgcaacgcgc cttgataaaa
     9121 cgggacggcg caatagaata cgacgggcag caccattaaa aagccgcccc aatggaggat
     9181 ttgcaggaaa tcgggttcga tgtcgccgcc gtaaaggtag gtcggcagcg cgaacatcat
     9241 cgtctgcatc atccccagcc cggcaacggc gaggcggacg atgtattgtt ttcgttcttt
     9301 ttggttggcg gcttcgattt tttgcgcgtc atagggtgcg gcggtgtagc ctatctgcct
     9361 gattttcaac agaatgtcgg aaaggcggat tttgccgtcg tcccagacga cgcggcagcg
     9421 gtgcgtgctg taattgaggt cgatgcggac gatgccgtct gtacgcaaaa gctgctgttc
     9481 gatcagccag acgcaggcgg cgcaggtgat gccgccgagc attaaaaccg cctcgcgcgt
     9541 gccgccgtgg gtttccacaa agtcggactg gacttcgggc aggtcgtaca ggcggatttg
     9601 gtcgaggatt tcttggggcg gcagctcggt tttttgcgcg tcggcggtgc gttgtttgta
     9661 ataactgccc aagcccgcgt caataatgct ttgtgcgacc gcctgacaac cggcgcagca
     9721 ggtttcgcgg tcttcgtttt cgtaacggac agtcaggtgg aggtgttcgg gaacatccag
     9781 accgcagtgg aaacaggttt ttttcatggc atttcggttt cgtctgtgtt tggtgcggtg
     9841 gcacaatact cggcaatggc ttcgcgcagt gcgtctatac cggtattttc agcaacggaa
     9901 atgcggacgg tggcaatttt tcccgcagcg tcgcgccata tgcccgtgtt ttgttcttca
     9961 gacggcagca ggtcggtttt gttgtacacc ttgatgcacg gaatatcgcc ggcatggatt
    10021 tcttgcagta cgttttccac gtcttcaatc tgctgtccgc tgttcggggc ggcggcatcg
    10081 acgacgtgca gcagcacatc ggcttgcgcg gtttcttcca gcgtggcgga aaatgccgaa
    10141 atcagtttgt gcggcaaatc actgacgaat ccgacggtat cggtcaggat aatgctgcat
    10201 tcgggactga tgtacagccg ccgcgccgtg gtgtcgagtg tggcgaaaag ctggtctttc
    10261 gcatatatgc ccgacttggt cagccggttg aacaggctgg atttgccgac attggtatag
    10321 ccgaccagcg caaacgtttt gattgtgccc gattcgcggg gcttgcggcg cagggcgcgc
    10381 tgttttttga ggttgacaag ctgttttttc aaggcattga tccgatgggc gatcaatcgg
    10441 cggtcggttt ccagtttggt ttcgccgggg cctttcatgc cgataccgcc gcgctggctc
    10501 tgcagatggc cgtaaccgcg tatcaagcgt cccgccaaat ggctcaattg cgccaactcg
    10561 acttgcagcc tgccttcctg cgtgcgggcg cggcgggcga aaatcgccag aatcagccct
    10621 accctgtcca atacgcggca tttcagttct ttttcaaggt tgcgttcctg cgtgggcgtg
    10681 agttcgtggt tgaatacgac caaatcgatg ccgtctgcgg caactgcttc tgacagctcc
    10741 gccgccttgc ccgtgccgac aaacagcgcg gtgtgcggac ggtcgcgttt ggcagtctcc
    10801 acgcgcacgg aatcgccgcc cgccgctttg accagctcga cagcttccgc caatgccgtc
    10861 tgaaaaccgt tcagacgggt ggcactgctg cccgtaccat ctttgtccaa cattacgccc
    10921 accagcatga cgcgttcggg ttgcgcttgg gtactgccgt tgccgcctgt tctgcctgtc
    10981 atgtattcaa tcctgtaaat aaagggttac gcctcgttcc gtttctcctt ccggtgcaag
    11041 cgtgatgcgg ctgcccgcca ttccgtcaac gctgaagacg atgccgcccg cctcgtgcct
    11101 tcctatggac aggcggctgc cgggtcgctg ccggtaggta taacgggtac acgcttccaa
    11161 acaccagcgt ccgcgccagt ggatgaaata tttttcgcct tttctaaccg caagtgtctc
    11221 gccgccgttt acctcaaaat aagggtaatc gggttttcgg gttctaaact gccatttcgc
    11281 ctgcgcgcgc cgtccgttgc ggacatagtc gaataccgcc gtataaagcg tgccgtattc
    11341 caaaggcttg agcggaaaaa gcgcgaattg gtacgcggtc agcctgccgt tggggtcgtt
    11401 gccggcggtt aaaaccctga cggggcggat ttcgttttta ccctgataca gcttgaaact
    11461 tttcatcgta attttgcctg ccgcctcgga aaaatcaatg ctggcaggat tgcccgtgat
    11521 ttcatattcc ggcacggggt ctggacgctc gccgtggaaa taaggcagtg cgccgctgcc
    11581 gacgggatag gctgtataga gcagctcctg tgcgggcatg gcttcgtcgg tgtacacgac
    11641 cgcaccgtta tggcaggcgt tgcggtaata tttccgtcct gcttccggct gatttctgcc
    11701 ttgggcgcaa tgccgctcaa acctgctgtt gccctgattg aatacgagaa cggttttacc
    11761 gttttcgcgc acaaatgccg ctcctgcctc atccgtatgg cggtcaagta gggaaaggcg
    11821 gtggtagatt gcgctcatta atccgtccac ttggcgttgc tgcgtgcgga tgtcgctgtc
    11881 ggacgattcg gcggcttcct cttccgtgct gatgttttca tgcacgccgt tgtagagata
    11941 cccggcaagg cgtgtgcgtt cggtcagctt ttgtgcggtg tagtgcggat tgtcgggatg
    12001 gtgttcgccg tgtccgtctt cgggattgag cgtgagatag cgtgcgtgcc tgcgggcgga
    12061 attttccaaa accggcgcgt gtgccagctt gtgcaaaccg atttgggcgc ggatgcggtt
    12121 gaggtagtgc agcgcatcgc cgccgtccct gatttgcggt gcggacggat agacaagttc
    12181 gcccgcgggc agggattggt tttgggtatg atagaaaacg cctgccgccg aacccaacag
    12241 gagcagccga ataaaaaggg atttcataat gccgtcctcc cgacgcggca acggggtgtt
    12301 ttatcaaaac ggcaaacttg ccaatgcggt ttccgattgc agattgccaa accggcaaac
    12361 ctttccccgt gccgatgccg aacctgatgc cgtctgaacc ttcagacggc atcggatgtt
    12421 tatttgtcca cccggatggg tgcaggttca tattgtgtcg attcgtcgcc gtaatacagc
    12481 acgccgagtt tgatggggat tctgccctgc gatttgcggt gggcattgga atcgcgcaag
    12541 gaataggcgc aaccgcaata ttcctgctgg tagaagtttt cacgtttgct gatttcaatc
    12601 atgcgcgcgc tgccgccgcc tttgcgccag ttgaaatccc aatacaccac atcatcgtaa
    12661 ggcgcggcgg cgcggtgtcc gcagtcgttg atttgcgcca tatttttcca gcgtgaaatg
    12721 cccagcgaac tggtaaagac ggggaacccg tgttcatgcg cgtattgcgc cgccttttca
    12781 aaacgcatat cgaaacacat ggtgcagcgg atgccgcgtt ccggctcaaa ctccatgcct
    12841 ttggctttgg caaaccattc tttgcggtcg ttttcgtagt cgtcgtcttt atcgatgaaa
    12901 ggaatgccga acttttccgc aaagcgcacg ttttcctctt ttcggagcat atactctttg
    12961 tgcggatgga tattgggatt gtaaaaataa atggtgtagt cgatgccgga ggcaagcatc
    13021 gcctccatca cttcgccgct gcacggggcg cagcaggaat gcagcaagac ttttttatgt
    13081 ccgccgggcg ggacgagtat ggggcggtca atgtcggtaa cggtaggttt gttttgtgtt
    13141 tccattaact taacaatatc tgttgaatac ggttgaagcg gctattttat cagagccgcc
    13201 gcatgtaaaa cagagtgtca gggcatcaac ccgagctgtc tgaacgaacg gacgctttgc
    13261 gaggtaacga taaaatggtc gagcagcgac acatcgacca gcgacattgc ctgtgccagc
    13321 cgccttgtga acatgatgtc ttcctgcgaa ggttcgggcg agccgcccgg atggttgtgc
    13381 gcgataatca ggctgtcggc atattcgtcc aatgccagtt tgacgatttc gcggatgtaa
    13441 atcgtgtttt ccgcaaccgt accgcgcgac agctctctga ccgcaatcag ttggttttgg
    13501 cggttcagca gcagcgcgac gctgacttcg actttttcct gccccaaatg aaagcgtaaa
    13561 taatcggcca ccgtatccgg atcggacagg acgatgctct cctgcaattc ttccgccaat
    13621 atccgccgcc cgatttccct gaccacggca aactgtgtga aacttgccgt ccccatccct
    13681 ttgtatgccg acagtttgcc gacctccgcg ctcatcagcc tccccaaact gccgaactcc
    13741 tgcagcaaat aacgcgccaa atcaaccgca ctcattccgc gcgtgccgac gcgcagcagg
    13801 attgccaaaa gttcggcatc actcaaagcc gccgccccgc gttccaacag cttttccctg
    13861 ggcctttccc cctccggcca ttgcttgatg ctcatgtttt ccctttttta aatagtctga
    13921 gatgccgtct gaaacaaatg ttcccacacg ccaaatatcc gaaatgccgt ctgaaccgga
    13981 tttccgcttc agacggcatc tgtatttcaa aacagtctta cgccgtttct tccaactcga
    14041 ttgaagccgc cagaagcgac aggcgtgcca ttacgccgta aacgtagagg cggttgtgtt
    14101 cgttgtccac gtcgcagtct ttttcagagc gcggcatacc cagcgagtcc cattgttcga
    14161 atacgcgctt gcaggcttcg ggcgcttctt gggaattatc gccgttaccg gtaggaatgc
    14221 tgttagacag cggaacaaac accataccgc cggcgttgag gttttcgtcc gcaccgcgcc
    14281 cttcgtgtac gcggaaaaag ccgccgatga cgaagcggtc catcatatac acgacgggtt
    14341 cgcacaccgc gctgtttaag gtttcataag tataaatccc ttcttggaca atcacttcgc
    14401 tgacttccaa accttctttg actttcgcca ttttattgcg gtttttacgg ttcaatccgc
    14461 gcacttcgtc ggcggatttg acgctcatca cgcccatgcc gtaagtgccg gcatcggctt
    14521 tgacgatgac gaaaggtttg tcggtaatgc ccgattcgtc gtatttggct tgaattttcg
    14581 ccagcacacg ttctaccgcc tccgccaacg cgtcttcgcc ttcacgccct tggaagtcca
    14641 aaccgccgat tttttcaaaa taagggttaa tttgccattc gtcgatgccg attaacttgg
    14701 caaattcggc ggcaacttgg ttgtacgcgc cgaaatgatt tgttttgcgg cgcgtcgtcc
    14761 aaccgccgtg caacggcggc aaaacggttt ggctgatgcc tttgaggatg tcgggaatac
    14821 cggcggacaa atcgttgttc aacaaaacca cgcaaggcga aaagccgtct gcaagatgga
    14881 cgcggtcgcg ggtacgcaat aaaggttcca acaggatttt gtcgcccaat gcggtttcaa
    14941 attcggtcgg ttcggttact tccggattca ggctgcccaa gcgcacttca taccctgccg
    15001 aacacaaaat ctcgccgagg gcgtaaacgt tttgcaggta aaacgtattg cgcgtgtggt
    15061 tttcgggaat aatcaatacg gatttcgccg tttcacaggc gcgttgcacc gcatcttgcg
    15121 cggcaaccgc cgccagcggg ataaagttgg gattcagatt attgaagccg ccggggaaca
    15181 aattcatatc gatagacgaa attttgtaac cggcattgcg tatatcgacc gaaccgtaaa
    15241 acggcgggcg gtgtacgctc cactgcgtgc ggaaccacgc ctcgattttg gcgtgattgg
    15301 acaggatttt tgcctcaaac gcctgaagtt gcgccgaatg ttcgggcgac atcaccggta
    15361 atttcatctt tcgtctgcct ctgtgtgttt tttgacaaga tgcagatgat aatgcctttg
    15421 aaagaatgac gacaagtatt ctttcgtatc gaatcatgct ttagatataa ctgtttactg
    15481 aaatggacaa tatgtctaat tttttcgtta aaattgtttg caaaataaca ccggctcaaa
    15541 aattagacaa aatttgttgc gcggtataaa gaatacgtct aaaatccgcc caatccgcat
    15601 taccatttat ccaaaagaac aacatcatga cagcacaaac cctctacgac aaactttgga
    15661 acagccacgt cgtccgcgaa gaagaagacg gcaccgtcct gctctacatc gaccgccatt
    15721 tggtgcacga agttaccagc cctcaggcat ttgaaggctt gaaaatggcg gggcgcaagc
    15781 tgtggcgcat cgacagcgtc gtctccaccg ccgaccacaa caccccgacc ggcgattggg
    15841 acaaaggcat ccaagacccg atttccaagc tgcaagtcga tactttggac aaaaacatta
    15901 aagagtttgg cgcactcgcc tattttccgt ttatggacaa aggtcagggc atcgtacacg
    15961 ttatgggccc cgaacaaggc gcgaccctgc ccggtatgac cgtcgtctgc ggcgactcgc
    16021 acacttccac ccacggcgca ttcggcgcac tggcgcacgg catcggcact tccgaagtcg
    16081 agcacacgat ggcgacccag tgcattaccg cgaaaaaatc caaatccatg ctgattgccg
    16141 ttgacggcaa attaaaagcg ggcgttaccg ccaaagacgt ggcactttac atcatcgggc
    16201 aaatcggcac ggcgggcggt acaggctacg ccgtcgagtt tggcggcgaa gccatccgca
    16261 gcctttctat ggaaggccgc atgactttat gcaatatggc gatcgaagcc ggtgcgcgct
    16321 caggcatggt tgccgtcgac caaaccacca tcgactatgt aaaagataaa cccttcgcac
    16381 ccgaaggcga agcgtgggac aaagccgtcg agtactggcg cacgctggtg tctgacgaag
    16441 gtgcggtatt cgacaaagaa taccgtttca acgccgaaga catcgaaccg caagtcactt
    16501 ggggtacctc gcctgaaatg gttttagaca tcagcagcaa agtgccgaat cctgccgaag
    16561 aaaccgatcc ggtcaaacgc agcggtatgg aacgggccct tgaatacatg ggcttggaag
    16621 ccggtacgcc attaaacgaa atccccgtcg acatcgtatt catcggctct tgcaccaaca
    16681 gccgcatcga agacttgcgc gaagccgccg ccatcgccaa agaccgcaaa aaagccgcca
    16741 acgtacagcg cgtgttaatc gtccccggct ccggtttggt taaagaacaa gccgaaaaag
    16801 aaggcttgga caaaattttc attgaagccg gttttgaatg gcgcgaaccg ggctgttcaa
    16861 tgtgtctcgc catgaacgcc gaccgcctga ccccggggca acgctgcgcc tccacctcca
    16921 accgcaattt tgaaggccgt caaggcaacg gcggacgtac ccacctcgtc agccccgcta
    16981 tggcagccgc cgccgccgtt accggccgct ttaccgacat ccgcatgatg gcgtaagtat
    17041 tttcaggcgg tctttaagaa tttgacgcgg ccgtctgaaa tatgtcccgt aaggcagaaa
    17101 cacctttaat attcaaacca aggagtttaa ccatgaacaa acttttcatt accgccctgt
    17161 ccgccctcgc cttgtccgcc tgcgccggca cttgggaggg cgcgaaacaa gacaccgccc
    17221 gcaaccttga caaaacacag gccgccgccg aacgcgccgc cgaacaaaca ggcaacgccg
    17281 tcgaaaaagg ctgggacaaa accaaagaag ccgtcaaaaa aggcggcaat gccgtcggac
    17341 gcggcatttc ccatctcggc ggaaaaatcg aaaacgccac cgaataaccg attcgcgatg
    17401 ccgtctgaaa ccgcttcaga cggcatcgtc agcacaagac gcaaacccat gaaagccttt
    17461 accaaaatta ccgccatcgt cgccccgctc gaccgcagca acgtcgatac cgacgccatc
    17521 atccccaaac aatttctgaa atccatcaaa cgcagcggtt tcggccccaa tgcctttgac
    17581 gaatggcgtt acctcgacca cggcgaaccg ggcatggaca acagcaaacg cccgttgaac
    17641 cctgatttct cgctgaacca gccgcgttac caaggcgcgc aaatcctgtt gacgcgtaaa
    17701 aacttcggtt gcggctcttc acgcgaacac gccccttggg cattggacga ctacggcttc
    17761 cgcgccgtca tcgcccccag cttcgccgac atcttcttca acaactgcta caaaaacggc
    17821 cttttaccta tcgtgttgac cgaagaacaa gtcgatcggc ttttcaaaga agttgaagcc
    17881 aacgaaggct atcagctctc catcgacctt gccgaacaaa ccctgaccac ccctggcggc
    17941 gaaacattca cattcgacat taccgaacac cgcaaacact gcctcttaaa cggcttggac
    18001 gaaatcggac tgaccctgca acatgccgat gaaattcacg cctttgaaga aaaacgccgc
    18061 caaagccagc cttggctgtt taacggttaa aacctatgcc gtctgaaggc cgttcagacg
    18121 gcatttctga taacatataa ttgctgcaac tgatgtaaac agagggatta tcaattgctt
    18181 tcatctttca agcgggctta gcaacgctct aaattaatgc aatacaactc tatttcaaat
    18241 aaatttttag atttttaata tgcaacaaat taaatttatt gacttatttt ccggaatgag
    18301 tggcatcagg aaaggattcg aacaagcctg tcggaaacag tcggttgctt gcgaatgtgt
    18361 ttttacttca gaaatcaaac cggcagcttt ggaagtattg aagcaaaact accctgatga
    18421 agtgccgtat ggagacataa cgaaaattga aacaggggat attcccgatt ttgacattct
    18481 gttggcaggc ttcccttgtc aggctttttc tttcgccgga aaaagattgg gctttgaaga
    18541 tacacgggga acgcttttct ttgatgtggc aaggatttta aaagcaaaaa aaccaaaagg
    18601 ttttattttg gaaaatgtgg aaggattggt gacacacgat agaaaagatt cgacacaaaa
    18661 atagggcgca ccctgaccgt tattttggaa accttggaag cattgggcta ctatgtttct
    18721 tggaaagttt taaatgcaaa agatttcggc attccccaaa acaggaagcg catttatctg
    18781 acaggcagtt tgaaatccaa acccgactta tctttcgaaa caagcccaag tccgaaatta
    18841 aaaaatattt tggaatcggg actgcctact gaaagcagcc ctttcatcaa gaaattgcta
    18901 aaaaaatttc ccccgtccga actgtacgga aaatcagtaa aagacaaacg gggagggaaa
    18961 aacaatattc acagttggga tattgaatta aaaggcgcag taaccgaaga agagaagcaa
    19021 ttgttaaata tccttctaaa agaacggagg aaaaaaaatg ggcttcagaa atcggcatag
    19081 attggatgga tgggatgcct ttgacaaaag cgcaaatttc aactttctat aaacaccccg
    19141 atctccaaaa tattttggac agcctgacag ataaaggcta tttggtttta gagcatccga
    19201 aacaaaaaat tggcgggcaa agaatcaaag atgaatccct gcccaaaggc tacaatattg
    19261 tttcaggtaa aaaatctttt gaaatcaata aaatattaga tccaaacgat gttgcgccaa
    19321 ccttggttgc aatggatatg gagcaccttt tcgtcgttga caacggcggt ttgcgtacac
    19381 ttaccggaaa agaagggttg cgcttattcg gctatccgga cgattattcg tttgatattc
    19441 ccaaaaaaga cagatgcgat ttattgggta ataccgttgc cgtccctgtg attaaggcgg
    19501 tatctgaaag acttctgcat actttataaa ccgacaggga taaaatgata aaacttactg
    19561 cacaacaaat atttgataag ttgctggatg aagaaaaaat cttatcggcc aatggtcaaa
    19621 ttagattttt cttgggcgat gtggatatta tcgtcaaaca aaaagatgtt gtcggcaaca
    19681 tcattcagga atggctcggc ggatgattga gaaaaaggga aattgaattt gatgtttcaa
    19741 ccaataccca aatgcctccc gatttctttt taaataaaaa agaccgcagc agagaattgt
    19801 tggaagtaaa ggcgttcaac cgaaataccg gcccgggttt tgatattgca gattttaaaa
    19861 tgtattctga tgaaatcatt cataagccct atatgctgga tgtagactat ttaatattcg
    19921 gttacgatat ggacgacaac ggcaatgtaa ccatcaagga tttatggctt aaaaaagtat
    19981 ggcaaattac gagaagcatg gatgggtggg caattaatct tcaagtcaaa aaaggcgtgg
    20041 tgcataaaat ccgcccgggt gtttggtaca gcataaataa aaagaatatg cctatgtttg
    20101 aatgcttgga agattttgtt tccgcaattg aagaaaccgt ttatcaaaac ccggcaacac
    20161 ggcataacgc atccttatgg aaaaagaaat ttgaggaagc ctataaaaaa cactacaacc
    20221 gatcgatttc catacctcgt tggcacgaaa ttgcacacaa atacaaaaag aaataaccct
    20281 tcaaccatca acccataagg aaacttgaaa tgaccaaaca tatcgccatc ctccgaggtg
    20341 acggcatcgg tcccgaaatc gtcgccgaaa ccgtccgcgt actcgacaaa ctcatcgccc
    20401 aaggtttgga tgccggctac gaatacgccc cattgggcgg cgaagcctat gacgaatacg
    20461 gtcatcctta tcccgaattt acgcaaaacc tctgccgcaa agccgatgcc gtcctgcttg
    20521 gtgcagtcgg ttctcctcaa tacgacaacc tcgaccgtcc gttgcgccct gagcgcggcc
    20581 tgttggcaat ccgtaaggat ttgaacctgt ttgccaattt gcgtcctgct gttttatatc
    20641 ccgaattagc caacgcctcc acgctgaagc ctgaaatcgt tgccggtttg gatattttga
    20701 ttgtgcgcga actcaccggc gacatctatt tcggcgaacc gcgcggcatc cgcgttctgg
    20761 aaaacggcga acgcgaaggc tacaacacca tgaaatacag cgaaagcgaa atccgccgta
    20821 tcgcccacgt cgctttccaa tccgcccaaa aacgcagcaa aaaagtctgc tccgtcggca
    20881 aagccaacgt tttggaaacc accgaactgt ggcgcgaaat ttttgaagaa atcggcaaac
    20941 aataccctga tgtcgagctt tcccatatgt atgtggacaa tgccgctatg cagttggtac
    21001 gcgcgcccaa acaatttgac gtgattgcca ccggcaacat cttcggcgat attttatcgg
    21061 acgaggcctc tatgctgacc ggctccatcg gtatgctgcc ttctgcttct ttggacgaaa
    21121 acggcaaagg cctgtacgaa ccgtctcacg gctccgctcc cgacatcgcc ggacaaaaca
    21181 aagccaaccc gctggccacc atcctctcgc tcgcaatgtt gctgcgttac agcctgaacg
    21241 acgaagcgcg cgcgcaacaa gttgaaaacg ccgtccaaaa agtgctgcaa caaggcttgc
    21301 gtaccagcga catttacgaa gaaggcacaa aactcgtttc ctgctccgaa atgggcgatg
    21361 cggtactcgc cgccttgtaa aaaaacaaag cgcggatcac accgcgcttt ttcatctgcc
    21421 cttttccgct cgccccgttc gtcaaccgcc tgcaaagcca ccgtttaccg gcaggaaatg
    21481 ccataaatcc ccgcctatgt taggataaac gcctttactg gctcacacaa aggattatca
    21541 tcatgaaaaa aatcatcttc gccgcactcg cagcggcagc cgttggcact gcctccgccg
    21601 ccacctacaa agtggacgaa tatcacgcca acgcccgttt cgccatcgac catttcaaca
    21661 ccagcaccaa cgtcggcggt ttttacggtc tgaccggttc cgtcgagttc gaccaagcaa
    21721 aacgcgacgg taaaatcgac atcaccatcc ccgttgccaa cctgcaaagc ggttcgcaac
    21781 actttaccga ccacctgaaa tcagccgaca tcttcgatgc cgcccaatat ccggacatcc
    21841 gctttgtttc caccaaattc aacttcaacg gcaaaaaact ggtttccgtt gacggcaacc
    21901 tgaccatgca cggcaaaacc gcccccgtca aactcaaagc cgaaaaattc aactgctacc
    21961 aacgcccgat ggagaaaacc gaagtttgcg gcggcgactt cagcaccacc atcgaccgca
    22021 ccaaatgggg cgtggactac ctcgttaacg ttggtatgac caaaagcgtc cgcatcgaca
    22081 tccaaatcga ggcagccaaa caataaatct tgatgcgaat cagcacaaat gccgtctgaa
    22141 cgtccttcaa acggcatttg tgccgtcttc atcaaaccgc cccgccaacg gtaagccgat
    22201 atggcggagc acaatccgcc gaaagatatt tcagcggcag gcagggcttg ccggaaagtc
    22261 cggaaa
//
LOCUS       DAWEWP010000015        22220 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWEWP010000015 DAWEWP010000000
VERSION     DAWEWP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22220
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.015"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(22..1617)
                     /locus_tag="WGN53_000418"
     CDS             complement(22..1617)
                     /locus_tag="WGN53_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ0139450.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(1724..2119)
                     /gene="hisI"
                     /locus_tag="WGN53_000419"
     CDS             complement(1724..2119)
                     /gene="hisI"
                     /locus_tag="WGN53_000419"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ0139451.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRILMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(2150..2917)
                     /gene="hisF"
                     /locus_tag="WGN53_000420"
     CDS             complement(2150..2917)
                     /gene="hisF"
                     /locus_tag="WGN53_000420"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ0139452.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTCPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(2930..3667)
                     /gene="hisA"
                     /locus_tag="WGN53_000421"
     CDS             complement(2930..3667)
                     /gene="hisA"
                     /locus_tag="WGN53_000421"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ0139453.1"
                     /translation="MLLIPAIDLKEGRCVRLRQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(3700..4338)
                     /gene="hisH"
                     /locus_tag="WGN53_000422"
     CDS             complement(3700..4338)
                     /gene="hisH"
                     /locus_tag="WGN53_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ0139454.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(4469..5971)
                     /gene="pta"
                     /locus_tag="WGN53_000423"
     CDS             complement(4469..5971)
                     /gene="pta"
                     /locus_tag="WGN53_000423"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ0139455.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(6156..7214)
                     /gene="fbpC"
                     /locus_tag="WGN53_000424"
     CDS             complement(6156..7214)
                     /gene="fbpC"
                     /locus_tag="WGN53_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ0139456.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(7235..8752)
                     /gene="fbpB"
                     /locus_tag="WGN53_000425"
     CDS             complement(7235..8752)
                     /gene="fbpB"
                     /locus_tag="WGN53_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ0139457.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(8820..9815)
                     /gene="fbpA"
                     /locus_tag="WGN53_000426"
     CDS             complement(8820..9815)
                     /gene="fbpA"
                     /locus_tag="WGN53_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ0139458.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(10066..10536)
                     /locus_tag="WGN53_000427"
     CDS             complement(10066..10536)
                     /locus_tag="WGN53_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ0139459.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGSILQFVCNWSFPAAEKTCSVLKKIGAVHSAMLIHRAADALGKEIRHLQS
                     EGKNLKEMWDITQHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(10594..11970)
                     /gene="argH"
                     /locus_tag="WGN53_000428"
     CDS             complement(10594..11970)
                     /gene="argH"
                     /locus_tag="WGN53_000428"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ0139460.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSLDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDFERMTDCRKRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELMRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(11989..12858)
                     /gene="galU"
                     /locus_tag="WGN53_000429"
     CDS             complement(11989..12858)
                     /gene="galU"
                     /locus_tag="WGN53_000429"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0139461.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYERSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTK"
     gene            complement(12886..13485)
                     /gene="rdgB"
                     /locus_tag="WGN53_000430"
     CDS             complement(12886..13485)
                     /gene="rdgB"
                     /locus_tag="WGN53_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ0139462.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFEPYGITVLPQSEFGIPECP
                     EPYPTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCSYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPEHGKTAAELDAEVKNRESHRAQALADLIRKLAL"
     gene            complement(13478..13711)
                     /locus_tag="WGN53_000431"
     CDS             complement(13478..13711)
                     /locus_tag="WGN53_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139463.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAIASFLFA
                     FGALFGQIGALALYLRHKSLRAAQSAAQENRYV"
     gene            complement(13819..14352)
                     /locus_tag="WGN53_000432"
     CDS             complement(13819..14352)
                     /locus_tag="WGN53_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ0139464.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGGVE
                     EAKKVIKESIERWNKQA"
     gene            complement(14454..14912)
                     /gene="nudB"
                     /locus_tag="WGN53_000433"
     CDS             complement(14454..14912)
                     /gene="nudB"
                     /locus_tag="WGN53_000433"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ0139465.1"
                     /translation="MPMAKPLKYPVSALVVLHDGDGSILLIERTHPQGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDRHDSTVYEIYHHWRHRYPKGVFENREHVFW
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            15121..15197
                     /locus_tag="WGN53_000434"
     tRNA            15121..15197
                     /locus_tag="WGN53_000434"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15155..15157,aa:Pro,seq:ggg)
     gene            15355..17181
                     /gene="mafB"
                     /locus_tag="WGN53_000435"
     CDS             15355..17181
                     /gene="mafB"
                     /locus_tag="WGN53_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ0139466.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VSDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            17184..17648
                     /locus_tag="WGN53_000436"
     CDS             17184..17648
                     /locus_tag="WGN53_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139467.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            17837..18019
                     /locus_tag="WGN53_000437"
                     /pseudo
     CDS             17837..18019
                     /locus_tag="WGN53_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
     gene            18031..18318
                     /locus_tag="WGN53_000438"
     CDS             18031..18318
                     /locus_tag="WGN53_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ0139468.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            18814..20070
                     /locus_tag="WGN53_000439"
     CDS             18814..20070
                     /locus_tag="WGN53_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0139469.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNK
                     WQGTSKSGIKIEGFTEPNRTAYPIYE"
     gene            20101..20520
                     /locus_tag="WGN53_000440"
     CDS             20101..20520
                     /locus_tag="WGN53_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139470.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            20598..20681
                     /locus_tag="WGN53_000441"
                     /pseudo
     CDS             20598..20681
                     /locus_tag="WGN53_000441"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20678..20920
                     /locus_tag="WGN53_000442"
     CDS             20678..20920
                     /locus_tag="WGN53_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ0139471.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            20998..21243
                     /locus_tag="WGN53_000443"
     CDS             20998..21243
                     /locus_tag="WGN53_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0139472.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            21246..21635
                     /locus_tag="WGN53_000444"
     CDS             21246..21635
                     /locus_tag="WGN53_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139473.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            21853..22125
                     /locus_tag="WGN53_000445"
     CDS             21853..22125
                     /locus_tag="WGN53_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139474.1"
                     /translation="MPIDNIPFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALT
                     GIAFLRGIDVYDPPVSKEKALKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT         5277 a         5029 c         6324 g         5590 t
ORIGIN      
        1 cattttattg ccgattgttt tttaaagttt gaccgaatgt tcgcgcgttt cgtggaacac
       61 gatgtccggc caacgttctt gcgtcaaccc caaattcacg cggttggggg cgaggtaggc
      121 gaggttgccg cccgcgtcga tggcgaggtt gcccgcgttg gctttttcaa actccgccag
      181 ttttttcttg tcgtcgcacg atacccagcg cgccgaccag atggatgcgc tgtcgaacac
      241 ggcttctacg ccgtattcgt tggcgaggcg cgaggttacg acttcaaact gcaacacgcc
      301 gaccgcaccc aaaatcaaat ccgcgccgct catcggtttg aacacctgta ccgcgccttc
      361 ttcgccaagc tgttgcaaac ctttttgcag ttgcttgatt ttcagcgggt ttttgatgcg
      421 gacgctgcgg aacagttcgg gcgcgaagaa gggaatgcct gtaaatgcca gttgttcgcc
      481 ttcggagaag ctgtcgccga tttggatgtt gccgtggttc gggatgccga taatgtcgcc
      541 ggcgtaggct tcttcaacca gctcgcggtc gtgggacatg aaggttacca cgctggaggc
      601 ggcgatttcg cggttgatac gcaggtgttt cattttcatg ccgcgctcga atttgccgga
      661 gcagacgcgc aagaaggcaa tacggtcgcg gtgtttcggg tccatattgg cttggatttt
      721 gaagataaat ccggaaaact tcggctcgtc cggctcgacc atacgcacgg tcgcatcgcg
      781 tggtttcggc gcgggcgccc attcaatcaa tgaattgagg atttcctgaa taccgaagtt
      841 gttaatcgca gagccgaaga atacgggcgt gagttcgccg gcgaggaatt cgtcgagatt
      901 gaactcgttg gaagccgcct gcaccaattc gatttcgtcg cgcaactgtt ggatttccaa
      961 tggaaagcgt tgttccaatt cgggattgtc tatgcctttg atgatgtcga actcgtgcgg
     1021 caggcgttcg ccgcccgctt caaagagata gatttcgtcg ttcaggatgt ggtatacgcc
     1081 tttgaagttt ttgcccatac cgatcggcca agtaacgggc gcgcagcgga tttgcaggat
     1141 gttttccact tcgtccaaga gttccagcga atcgcgcact tcgcggtcgt atttgttcat
     1201 aaacgtaaca atcggcgtat cgcgcaggcg gcagacgttt aagagcttga tggtttgcgc
     1261 ttccacacct tttgccgcgt caatcaccat taatgcgctg tccacggcgg ttaaaacgcg
     1321 gtaggtgtct tcggagaagt cttggtgtcc cggcgtgtcc aagaggttga cggtgtggtc
     1381 tttatagtcg aactgcatca cgcttgatgc cacggaaatg ccgcgctgct tctcgatttc
     1441 catccagtcg gaagtggcga atttgccggt ttttttgcct tttaccgtac ccgcgctttg
     1501 aatcgcgcct gaaaacagca agagtttttc ggttagcgtg gttttacccg cgtcagggtg
     1561 ggagatgatg gcaaacgtgc ggcggcggcg cacttggtcg aggatttctt gggacatggt
     1621 tttctttgca aaaaggttca ggccgctttt cagacggccc ggacagtgtt tgagacggcg
     1681 aaattgtaca aaaaaatgcc tgataattca atgttggagg cggtcagtgc gtgctgccgt
     1741 aaatctcttt ttcgtctttc aggacggcat cggcggtttc ccacgcgctg ccacgccaga
     1801 ctttgtaaaa gcagctttct cgcccggtgt ggcaggcgat gccgccgttt tgggcgatga
     1861 gcatcacaat ggcgtcgccg tcgcagtcga ggcgcagttc gcggactttt tgcgtgtgtc
     1921 ccgactcttc gcccttcatc cattgttttt ggcgcgaacg gctgtaatag tgggcaaagc
     1981 cggtttcgac ggttttttgc agggcttcgg cgttcatcca cgccaccatt aaaatacgtt
     2041 tggtttcggc atcttgggcg atggcgcaaa ccaaaccttt ttcgtcaaat ttgacggctt
     2101 caagcaggtt tttatccata tttcctttca gacggcatgg tcggggcggt cagaggcgca
     2161 cttcgatgcc ggcttcgcgc atagcgcgtt tggcttcgcg gatggcgatt tccccgaaat
     2221 ggaaaacgcc ggcggcaagt acggcatcgg ctttgccttc ggttatgcct tcaatcaggt
     2281 gccggacatt gccgaccccg ccggaggcga tgacggggat gtcgacggct tcggcaacgg
     2341 cgcgggtcag cggcaggttg aaaccctgtt tcgtaccgtc cctgtccata ccggtaagca
     2401 ggatttcgcc cgcgccgcgt ttttgcattt cgatcgccca ttccaccgca tccaaaccgg
     2461 tcggattgcg cccgccgtgg gtaaagattt cccagcgtgt gttttcgggg ttgacggctt
     2521 tggcatcgac ggcggcgacg atggcttgcg aaccgaaaaa tccggcggct tcgttaatta
     2581 aatcgggaca ggtaacggcg gcggtgttga tgctgacttt gtccgcgccg gcattgagca
     2641 ggcggcggat gtcggcaacg gtgcgtacgc cgccgccgac ggtcagcggg atgaagactt
     2701 gtccggcaac ctcttcgatg atgtgcagga tggtgtcgcg gttgtcggat gaggcggtga
     2761 tgtcgaggaa ggtcaattcg tccgcgcctt cgccgttgta gcgtttggcg gcttcgacgg
     2821 ggtcgcccgc gtcgcgcaaa ccgatgaagt tcacgccttt gacgacgcgt ccgtctttta
     2881 cgtcgagaca ggggatgatg cgttttgcca gtgccataat cggatgcctt tagtcgaggg
     2941 aatctgccag ttgctgcgct tgggcaaaat cgatgctgcc ctcgtaaatc gcgcggccgg
     3001 taatcgcgcc tgctacgcca tgtttttcgg cggcacacag ggcgcggatg tcgtccaagc
     3061 cggtcagtcc gccggaggcg atgacgggaa tgcggacggc ttgggcgagt ttgaccgtcg
     3121 cgtcgatgtt cacgccgctc atcataccgt cgcgcccgat gtcggtgtag atgatgctgt
     3181 tgacgccgtc gtcttcaaag cgttttgcca aatcaattac atgatgcccg gttacggttg
     3241 cccagccgtc gatggcggcc ataccgtctt tggcatccag cccgacaata atcctgccgg
     3301 ggaaggcttt gcacgcctcg cgcacgaagt cggggttttt gaccgccgcc gtgccgataa
     3361 tcacgtcgtt cagacccaaa tccaaatatt gtccgatggt tttcaaatcg cgtatgccgc
     3421 cgccgagctg tacggggatg tctttggcga cggcggcaag gatgtctttg atggcgggca
     3481 ggttttgcgg aacgccggca aacgcgccgt tcaaatctac cagatgcagg cggcgcgcgc
     3541 cttgtttgaa ccagtgcagt gcggtttcgg cgggcgaatc ggaaaagacg gtcgcctctt
     3601 ccatcagccc ttgtctcagg cggacgcagc gtccttcttt caaatcgatg gcgggtatca
     3661 gcagcataat ttttctcctt gtgcggggcc gtgtccggct taccagttta aaaagttttt
     3721 caacatcgtc agcccggcat cgtggctttt ttcggtgtga aattgcgtgg cgaatacgtt
     3781 gtctttgccg acgatgcagg caaacgggga cgggtagtcg ctttcgccca atatggtttc
     3841 gggattttcg ggggcgaaat agtagctgtg gacgaagtaa aaacgcgtgt cttggggaat
     3901 atctttaaac agcgggtggt tttgggtttg gcgcacggtg ttccagccca tatgcgggac
     3961 tttcagacgg catccctgcg ggtcgcggag gtcgcgctca aagcgtctga ctttgccgcc
     4021 gaaccagccc aagccgtcgg tgtttccttc ttcactgtgg tcgaataaaa gttgcgcgcc
     4081 gacgcagatt ccgaaaaagg gtttgttttt taaggcatct ttgactgcct cgtccaggcc
     4141 gccgcgcgtc agtgccgcca tacagtcggg catcgcgccc tgcccgggaa aaatgacttt
     4201 gtcggcgcgg gacacgcgtt cggggtcgcc gcttaaaaag atttcggtat tttttccggc
     4261 aagctgcccc gccgtccgga cggatttcaa tacggaatgc aggttgccca taccgtaatc
     4321 gataatggcg atttgcatgg cttcctcctc tttttttgca atatggctgc gattttaaca
     4381 aacaaatgtg ccgtgctgat aaaaatgccg tctgaaaacg ggagtctgtc ttcagacggc
     4441 atagggttta aacccggaaa gccgtttgtc agccttccat ttgttttgcc tgaacggcag
     4501 tcagggcgat ggtgaacacg atgtcttcca ccagcgcgcc gcgggagagg tcgttgaccg
     4561 gtttgcgcag gccttgcagc agcgggccga cgcttaagac gttggcactg cgttggacgg
     4621 ctttataggt gcagttgccg gtgttcaggt cggggaagac caaaacggtt gcctgtcctg
     4681 ccaccgggct gcccggagct ttggatttgc ccacacccgg cacggttgcc gcatcatatt
     4741 gcagcgggcc gtcgatggcg aggtcggggc gtttttcccg ggcaagtttg gttgcttcga
     4801 tgacggtatc gacatcgggg ccgctgccgg agttgacggt ggagtaggaa atcatcgcca
     4861 ctttcgggtc gatgccgaag gcttttgcgg aatcggcaga ctggatggcg atgtcggcaa
     4921 gctgttgcgc ggtcgggttc ggattaaccg cgcagtcgcc gaagacgagg acttggttgg
     4981 gcagcagcat aaagaatacg ctggacacga ggcttgcgcc cggtgcggtt ttaatcagtt
     5041 gcaaagcggg gcggatggtg ttggcggtgg tgtgaaccgc accggatacc aaaccgtcca
     5101 catcattttg cgccatcatc atcgtaccga gtaccacggt gtcttgcagt tgcttgcgcg
     5161 cgtcttcggg tgtcaggcct ttggatttgc gcagttcgca catcggctcg acgtattgtt
     5221 cgaccaatga ggcgggatcg atgatttcca aagagtcggg caggctgatg ccgcgttctt
     5281 tggcaacggc ttcgacttct tcgcgtttgg caagcaggac gcagcgggca atgccttttt
     5341 cgtggcagat ggcggcggct tggacggtgc ggggttctgc gccctcaggc aggacgatgc
     5401 gtttgtcggc ttggcgggcg aagtcgatca ggttgtagcg gaattgcgcc ggcgacaggc
     5461 gttttgcttc gcggcctgcc aatacggata cgtctttcag cgcgtcgctc gaaccgaaga
     5521 aggtcaggcc ggttttttcg gctgccgctt cggcaacgga ggctgccgcg ccgtccacga
     5581 caaaaccttc caatacgccc ggcgcggcgg cgaagaactg tttggcaagg ttcagccgat
     5641 ttgccagttc gtcggcatcg gcgttgtcgg aacggacggc gaagacggct gccgcgtcaa
     5701 gggacaatgc cagttcgacg tttttgcctg cgaggtagat tttgtcggca tcgggcgcga
     5761 tgccttcgat gacgaggttg gcggcatcga gtgcggcaac tttgccgacc agtgcgtcga
     5821 accagtcgtc gcttttgcct tgcgcgagca gggtttcggc ggttgcgtca acggcttgga
     5881 aaatttgtgc gtccagtgct tttgcaaagg cttgtgcggc ggcggaggcg tccagtccgg
     5941 cagatacggg tacgatgagt acttttgcca tgatatatcc tttcgtatgc tgcggtgtgc
     6001 ggcatatgtg gttggaaggg gcggcatata ggcagaaacg gctgcctgcg tgccgtgcgt
     6061 gccgtgtttg gcttgaggcg cgcaggttga atatagcaaa caaattctgt ttccaacaag
     6121 ataaatatcc gcaggcttgt ggatgctgcc gcctttcaga gggtatttcc ggggaagaac
     6181 agggcgggac cgtccaaatg gaggacggcg gaaatgccgt ctgacagggt gggggcggaa
     6241 gggaggttga gcgtgaggac ggtttgtccg acccggagac tgatttcggt atgccgcgct
     6301 ttgggcgtgg ttttgagaac cacggcgtga atggaggcgg tgggtgcgga atgggggtga
     6361 aggctgaact gttccggacg gatgagcagt gtgccgcgtg tgcctgcggg tgcgccgctt
     6421 tgaacgggca ggcggcccaa tccgcaatcg gcggtgccgt cggcgttgag cgcggcgggg
     6481 aacacgatgc cttcgccgat aaacagggcg gcatcaaggt cggcaggttg tcggtacaat
     6541 tcgtgagggc ttgcagtttg gaggatgcgc ccctgtttca tcacggcaat ccggtcggcg
     6601 tattgcaggg cttcttcgcg gtcgtggctg acgaaaacgg cagatttgcc gttggcgcgc
     6661 agggcggcaa tcatgtcttc gcgaatctgg cggcgcaact gttcgtccag cgcgctgaag
     6721 ggttcgtcca acaaaatcag ttcgggatcg ggtgcgaggg cgcgggcgag ggcgacgcgc
     6781 tgttgctgtc cgcccgaaag ttcgtgcgga tagcgtccgg caagttcgga aatgccggtc
     6841 aattccaaca tagcttcgat gcgctgccgc tcttgcgccg tcttgccttt gccgttgccc
     6901 agcccgtagg cggtgttgcg gtaaacggtc aggtggggga acagtacgcc ttcctgtacg
     6961 acataaccca aacggcgttc gcggacgggg aggttggtat ttttcgagaa gatggttctg
     7021 ccggaaagcg aaatttcgcc aaaatcgggt tgttcaaaac cggcaaggca gcgtaaaagg
     7081 gtggttttgc cgcagccgga cgcgccgacg ataaagagga tttcgcccgg gtcgaggctg
     7141 agcgaaatgt cgtttaaaac cggggtgttt tgaaaacttt tggacaggtg tccgatgtgc
     7201 agggcggcgg tcatggcggt acttcctcaa gctgttattt gaaggcgtat ttcttcagca
     7261 ggaatacggg gatgccggaa aataatacca gcatcagcgc gtaaggggtg gcggcggcgt
     7321 attgtgcgtc cgatgtgtat tcccaaacgg cggtggagag tgtgtggaca tcgtcggtgg
     7381 tcagcagcag ggtggcggtc agctctttca tcagtttgag gaagacgagt gcgaatgcgg
     7441 cggtaatgcc gggcaggatg gacggcagta ccaacgtcct gaaaataaag aagtgtccgc
     7501 gccccaatgt tgcgccgacc tgttccatcc ctttggggag ttgttccaag gaagtcctca
     7561 gggtggtttg cgccatcggc aggtaaagca tgaaataggc aaggatgacg acgataaagg
     7621 tttggtaaac ggcaggggtg tagttgatgc tgaaataaac caaggatagg gcgataacca
     7681 aaccggggac ggcgtgcagt aaaaacggca gcctgtctat ccaaacggtt aaaaaattgc
     7741 gatagcgaac cgatgcccaa acaaggggca aggcacataa tatagtcaaa atcgcaccta
     7801 aagccgatac gcttaaggaa cggataaagg catcaaatac ggatacgagc gcgaatgtgc
     7861 cggaagtgcc gaccatcatc caatgtatca atacgccaaa ggggataata atgcccaaag
     7921 tcaacaagct gcttaaaaaa acaatcgcgc caatctgacc gggcagtttg agggttttga
     7981 cgggataagg acgggcaacg cctttgccgc tgtggtaaat cttggctttg ccgcgaaata
     8041 tgctttctcc aaatacgacg atgccgcaca ccgccattaa aacagcggaa agcagggcgg
     8101 cggtattgtt gttgtaggac atttcgtatt cttggaaaat ggcggtggta aaagtggggt
     8161 agttcaaaat agataccgcg ccaaattcga ccagcatatg cagggcaatc agtaacacgc
     8221 tgctgccgat ggcgggtttg agctggggga ggatggcgga aaaaaaggtt tgcaggcggc
     8281 ttttgcccaa ggacaggctg acttcttcgt aagacaggct gatgcgtttg agtgccgcct
     8341 cgacgggcag gtaggcgagc gggaacgagg acaggctcat aatcatcact gtcccccaaa
     8401 agccttcgac acggaaggtc aggctgatcc aggtgaaaca gctgacaaat gcggggatgc
     8461 acaaaggcag ggtgattgcc gtctgaaaaa aggttttgcc gaagaagcgg taacgttgga
     8521 acaaaagggc gcaggcaatg cccaaaacaa tggaaatcag ggtaacgccc gccatcatcg
     8581 tcaaggtgtt ggagagcaaa tcccacatac gcgggcggaa caacagttcg acggcgcggt
     8641 tgatgccgac ctgccacgaa cgcatagcga catataaaaa aggcagggta agcggtaggg
     8701 caatcagtag gatgaggccg gtaagccaaa tgggtatttt tttaggagac atagtgtttt
     8761 ttatcggcaa aacgggcgga cagtataaat gtccacccgt ttgacaatcc gaaaacggct
     8821 tatttcatac cggcttgctc aagcagccgg gtggcgtgtt ctttttcgga aacagtggtg
     8881 gcggacactt ggggtgcttc caacttggcg atgggttcca aattgaaagt ggataccacg
     8941 tgcggattca aaggatattc ggcacggacg gcggtcaggg cgcgctgtcc ttccttgctg
     9001 gcgaggaagg cgacgaattt tttcgcctca tccttgtttt gggaggattt taacacggct
     9061 gcgccggaat aggtaacgag tgcgccggga tctctgtggc ggacgaaatt caggcgggtg
     9121 tggacatttt gtacgccttt ttcacgcgca aaagcgtgcc agtagtagtt gttgatgagg
     9181 gcggcatcga tttcgccgtt ttcaaccgct tgaagggcga cggagttttt agcgtaaggc
     9241 ttgccgtatt ctttcagacc tttgagccat ttcaatgcgg ccgcttcgcc tttcagtttg
     9301 acgatggcga caacctgttc caagaacgcg ccggaagtgg gggcgtaacc gatgcggttt
     9361 ttccatttcg gcgtggcgta attcaggacg gatttttcca aatctttttc agacagtttg
     9421 cgggtgtcgt aaacgacgac gcgcgaacgt ccgctcagtg ccacccagtc ttttttggcg
     9481 gcaaccggca cgcccttgcc gcgtgtttcg ttgatggtgg aggcgggcag gggctctagg
     9541 aggttggctg cggaaagggt ggcgagtgcc gggatttgtt cggaatagaa tacgtcggcg
     9601 gggcttcggc tgccttcttc tttgatttga ccggcaagct ggtcgccttt ggcgctgttg
     9661 agtttgactt tgatgccggt agcccgggta aaggcatctg caacggcttg tgccgcttct
     9721 ttgtgttggc cgttgtacac ggtaatgtct gccagcgcgg gggttgcggc ggtcagggct
     9781 gcggcaagca gtgcgtatcg gatagatgtt ttcatatcga ttttctccta atgaatgaga
     9841 gtgtacacct tgttaagaca taacggcgtg tagtgtattc cttcttcttt ataaatgcaa
     9901 ataattattt tttaaatttg ttattatccg atccggttat ggtttgttct gacttgtatt
     9961 ttttccgtgc atcgcgcccg taaggcggaa gcggcgggca atgcgtggcg gaatgtgggt
    10021 aaaggcggca ttttgatttg tcggaatgct tgagaacccc tctctttaaa acagcgtctg
    10081 aacagggttg ccggaatagt attgccatcc cggcagatac agtttgtcgg ggtcttgcca
    10141 atattgttca tccagactgt tcggcagtga ggcggttttg ttgtcgagat gttgcgttat
    10201 atcccacatt tctttcaggt ttttaccttc cgattggagg tggcggattt cttttcccaa
    10261 tgcgtctgcc gcccgatgga tcagcattgc gctgtgtacc gcgccgattt ttttcagaac
    10321 ggaacaagtc ttttcggcgg cggggaaact ccagttgcag acaaattgca gtatgctgcc
    10381 gttaccgata tcggcttctg tccgccaaat caaaaccagc tcctgttccc gcccgtccat
    10441 gctttcgagt ttgccgtcat gctgttcaaa aagtttgtcc accgcctgcc acatcatctg
    10501 ttcgaagcgg tcggcttggg tatccgtatc ggtcatcgtg ttcttgcctt ttaaaaatgc
    10561 cgtctgaaca tttcttcaga cggcatttgg gggttaagcc aacatttccc gccagcgttt
    10621 cacttggaag cggacttgtt ccggcgcggt accgcccaag tggttgcggg cgtttaagct
    10681 gccttcgggt gtcagcacgc cgtaaacgtc gtcggcaatc aaatcgctga aaccttgtaa
    10741 gacttcgagc ggcagttcgc tcaaatcgac gcccgcttgg tcggcgtggc gcacggcttg
    10801 ggcgacgact tcgtgggcat cgcggaaagg catgcctttt ttgaccagat aatccgccaa
    10861 gtcggtggcg gtagcgaagc cctgcatcac ggcggcgcgc atattgtcgg gtttgacggt
    10921 tacgccgcgc atcatatcgg cgtaaatccg caacgtgtcg ataagcgtgt cggcggtgtc
    10981 gaacaagggt tctttgtctt cctgattgtc tttgttgtac gccaagggtt gggatttcat
    11041 cagggtaatc agaccgataa ggtgtccgat gacgcggccg gatttgccgc gcatgagttc
    11101 gggcacgtcg gggtttttct tctgcggcat gatggacgaa cctgtgcaga aacggtcggc
    11161 gatgtcgata aagccgaaac gcgggctcat ccacaaaatc aattcttcag acaggcggct
    11221 caggtgaacc ataaccagcg aggcggcggc tgtgaactca atggcgaaat cgcggtcgga
    11281 tacggcatcg agcgagttct ggcagatttg ttcaaagccc aatagctcgg cggtgatttc
    11341 gcgctgaatc gggtaggtcg tcccggcaag ggcggctgcg ccgagcggca tacggttgac
    11401 gcgtttgcga cagtcggtca tgcgttcgaa atcgcgtccg agcatttcga cgtaggcaag
    11461 catatggtgc ccgaagctga cgggctgggc gacttgcaga tgggtaaaac cgggcataac
    11521 ggtttcggcg ttttgttccg ccaaatccag caatgccgtc tgaaggtttt gaatcaggtt
    11581 ttggataacg gtaatctggt cgcgcagcca caggcggatg tcggtggcga cttggtcgtt
    11641 gcggctgcgg ccggtgtgta ggcgtttgcc cgcgtcgccg attttgtcgg tcaggcggcg
    11701 ttcgatgttc atgtggacat cttccaaatc gagcgaccat tcgattttgc cgctgcggat
    11761 ttcttcgagg atttccgcca tgccccggcg gatgtccgcc aaatcgcctt cgtccaacac
    11821 gccggtttct ttcaacattt gcgcgtgggc gagcgagcct tggatgtccc attcggcaag
    11881 ccgtcggtcg aaaccgatgg aggcggtgta ttgtttgacg agttcggaaa cgggttcgtt
    11941 gaaacgtccg gaccaggttt tgtcgtgcat aaggattcct tgatggggtt atttggtacg
    12001 gtatttttcc aaaaggcggc ggaagggttc gccggtttcg gggtgtttta aaccgtaggc
    12061 gacggtggct tcgaggtagc ctagtttgct gccgcagtcg tagcgcgtgc cttcaaaggg
    12121 gtgcgccagg acaaattcgt gatcgagcag cttggcgatg ccgtctgtaa gctggatttc
    12181 gttgcccgcg ccgcgcggca gtccggtcag caagtcgaaa atgcgcgggg tgaggatgta
    12241 gcgtccaaca acggcaaggt tggagggcgc gtcttcgggc ttgggttttt cgacaatgcc
    12301 ggtaatgcgt tggaactgtt tgagctgttc ggtttcgacg atgccgtatg agccggtttg
    12361 cgacggttcg acggtttcta cgcccaaaat gctgttgccg ctgcgttcgt acacttcgac
    12421 catttgtttg agcgcgcctt ttggggcatc gattaagtcg tcagccaaaa tcacggcgaa
    12481 gggttcgtcg ccgatggcgg cgcgggcgca caagacggcg tgtccgagcc ccagtgcttc
    12541 ggcctggcgg atgtagaggc aggtaatgtt cggcggcagg atgttgcgga cgtgttccag
    12601 aagtttgtct ttatggcgca tttccaactc ggtttcgagt tcgtatgcct tgtcgaaatg
    12661 gtcttcgatg ctgcgtttgt tgcgtccggt tacaaacacc atttccgtgc agccggcttc
    12721 cacggcttct tctacggcgt attggatcag cggcttgtcg acgatgggca gcatttcttt
    12781 cgggctggcc ttggtggcgg gcaggaagcg ggttcccatc cctgcgacgg ggaaaacggc
    12841 tttttttatc ggtttcattc tttttccttt gtattgtttt gatgtttaaa gggcgagttt
    12901 gcggattaaa tcggcgagtg cctgcgcgcg gtggctttcg cggtttttga cctccgcatc
    12961 caattcggcg gcggttttgc cgtgttcggg caggtaaaaa tacgggtcgt aaccgaaacc
    13021 gttttcaccg gcgggaacgc cgttccactg cccgtgccat acgccttcgg caataatcgg
    13081 gcgcgggtcg tctttatggc ggacaaaaac caatacgcag acataagaac agcttttgtc
    13141 ggctttgccg gcaagttcgg cggcaagttt caggttgttg gcggtatcgg atttgggatt
    13201 gctgcccgcg taacgtgcgg aatggatgcc cggcgcgccg tttaaggcgg cggcacagat
    13261 gccgctgtcg tcggcaagcg cgggcagtcc gctgtattcg gcggcatggc gtgcttttgc
    13321 cagcgcgttt tcgacaaagg tgggataggg ctccggacat tcgggtatgc cgaattcgga
    13381 ttgcggcaat acggtaatgc cgtatggttc gaataggttg ccgaattctt tgagtttacc
    13441 tgcattgccg cttgccaaaa cgattttttc cggtttttca gacatagcgg ttttcctgtg
    13501 cggcggattg ggcggcgcgt agggatttgt gccgcaggta gagggcgagt gcgccgattt
    13561 gcccgaacag cgcgccgaat gcgaaaagaa aggaggcgat ggcgaaggct ttgctgccga
    13621 cggtcagcag ataagcaccc aagaggacga gcagcataaa tgccagtgtg aagagggcaa
    13681 cagacaatag atagattttt cggcggttca tggcgttcgg tcggaagcgg tatgttcgga
    13741 ttatatccga ttgggacggt attccctagg gcttggaaaa atgccgtctg aagcggcgtt
    13801 caaacggcag ggagggcgtt atgcctgttt gttccaacgt tcgatggatt ctttgatgac
    13861 ttttttcgct tcttccacac cgccccagga ttcgactttg gtcgtacctg cttttttcag
    13921 gtctttgtag tggttgaagt ggaactcgat ttgtttgatg agctgttgcg gcaggtcgga
    13981 caaagttttg taggcgttgc cgttgttgcg gtcgtcggca ggtacgcaga cgattttgtc
    14041 gtccacttcg ccgtcatcga cgaatttcat cacgccgata acgcgcgctt ccaagaatac
    14101 gccggttgcc agaggctgtt cggtaacgag caatacgtcc aattcgtcac cgtcttcgtc
    14161 taatgtttgg ggaatgaagc cgtagttggt cggtttggcg aagatggcgg gttcgacgcg
    14221 gtcgagttgg aatgcggcga gtttgcggtt ccactcgatt ttgtggttgc tgccggcggg
    14281 gatttcgttg acaacgttga tgatgccgcc gtccacgtcg ccgggggtca ggatttggtt
    14341 gaagtctgcc atttggtttc ctttatttgg gaaagtttga agtttgaaag tatagcacaa
    14401 acgtccggct gaaaatgcgc ccgatgccgt ctgaaagggt gtccgggcgc gtgttaccgt
    14461 ttgcccaaaa acctgcccag ttccagaatc gcgcgcctgt tggacgggga aaacactttt
    14521 tccgccgctt cttccaagcc gaaccagccg taggagacgt gttcctcggg ttgcaggacg
    14581 acgggcgtat cacgcgggat ttcggcccag aagacgtgtt cgcggttttc aaacacgcct
    14641 ttggggtagc ggtgccgcca gtggtggtag atttcgtaaa ccgtgctgtc gtgccggtct
    14701 tggagctgcc cgtccgccag caggatgccg gtttcttccc aaacttcgcg ccttgccgtt
    14761 tgtgcgacgg tttcgcccgg ttcgaggctg ccggttaccg actgccaaaa cccttgcgga
    14821 tgcgtgcgtt cgatgaggag gatactgccg tccccgtcat ggagtacgac cagtgcggag
    14881 acggggtatt tgagcggttt tgccatcggc atctttcggc gggctgaggt aatgaagggg
    14941 cggattatag caaacgccgc acgttatggc gtttatcctt ttccgcatcc ttttccgccc
    15001 gggcgggacg cgctggcgtt tgccggtaaa ttttccgttg tgtcaaaaag ataagggcgg
    15061 ttgtgatttt aatgcttgcc aaagcgtcgg gcggaaacta taatccgcaa cttatcgagt
    15121 cggagtgtgg cgcagtctgg tagcgcactt gcatggggtg caaggggtcg aaggttcgaa
    15181 tcctttcact ccgaccaaaa attccgaaag ccgctttcaa aagcggcttt tttgccgtcc
    15241 gtatgattat gatgtagagt acgcggcgac agacattcaa atgccgtctg aaaaccgttc
    15301 agatggcatc tctttatctt agtttcattc cgtaccatct taaggaacat caaattgggc
    15361 atttcccgca aaatatccct tattctgtcc atactggcag tgtgcctgcc gatgcatgca
    15421 cacgcctcag atttggcaaa cgattctttt atccggcagg ttctcgaccg tcagcatttc
    15481 gaacccgacg ggaaatacca cctattcggc agcagggggg aacttgccga gcgcagcggt
    15541 catatcggat tgggaaaaat acaaagccat cagttgggca acctgatgat tcaacaggcg
    15601 gccattaaag gaaatatcgg ctacattgtc cgcttttccg atcacgggca cgaagtccat
    15661 tcccccttcg acaaccatgc ctcacattcc gattctgacg aagccggtag tcccgttgac
    15721 ggatttagcc tttaccgcat ccattgggac ggatacgaac accatcccgc tgacggctat
    15781 gacgggccac agggcggcgg ctatcccgct cccaaaggcg cgagggatat atacagctac
    15841 gacataaaag gcgttgccca aaatatccgc ctcaacctga ccgacaaccg cagcaccgga
    15901 caacggcttg ccgaccgttt ccacaatgcc ggtagtatgc tgacgcaagg agtaggcgac
    15961 ggattcaaac gcgccacccg atacagcccc gagctggaca gatcgggcaa tgccgccgaa
    16021 gccttcaacg gcactgcaga tatcgtcaaa aacatcatcg gcgcggcagg agaaattgtc
    16081 ggcgcaggcg atgccgtgca gggtataagc gaaggctcaa acattgctgt catgcacggc
    16141 ttgggtctgc tttccaccga aaacaagatg gcgcgcatca acgatttggc agatatggcg
    16201 caactcaaag actatgccgc agcagccatc cgcgattggg cagtccaaaa ccccaatgcc
    16261 gcacaaggca tagaagccgt cagcaatatc tttacggcag tcatccccgt caaagggatt
    16321 ggagctgttc ggggaaaata cggcttgggc ggcatcacgg cacatcctgt caagcggtcg
    16381 cagatgggcg cgatcgcatt gccgaaaggg aaatccgccg tcagcgacaa ttttgccgat
    16441 gcggcatacg ccaaataccc gtccccttac cattcccgaa atatccgttc aaacttggag
    16501 cagcgttacg gcaaagaaaa catcacctcc tcaaccgtgc cgccgtcaaa cggcaaaaat
    16561 gtcaaactgg cagaccaacg ccacccgaag acaggcgtac cgtttgacgg taaagggttt
    16621 ccgaattttg agaagcacgt gaaatatgat acgaagctcg atattcaaga attatcgggg
    16681 ggcggtatac ctaaggctaa gcctgtgtct gatgcaaaac cgagatggga ggttgatagg
    16741 aagcttaata aattgacaac tcgtgagcag gtggagaaaa atgttcagga aataaggaac
    16801 ggtaataaaa acagtaactt tagccaacat gctcaactag agagggaaat taataaacta
    16861 aaatctgccg atgaaattaa ttttgcagat ggaatgggaa aatttaccga tagcatgaat
    16921 gacaaggctt ttagtaggct tgtgaaatca gttaaagaga atggcttcac aaatccagtt
    16981 gtggagtacg ttgaaataaa tggaaaagca tatatcgtaa gaggaaataa tagggttttt
    17041 gctgcagaat accttggcag gatacatgaa ttaaaattta aaaaagttga ctttcctgtt
    17101 cctaatacta gttggaaaaa tcctactgat gtcttgaatg aatcaggtaa tgttaagaga
    17161 cctcgttata ggagtaaata aaaatggcaa tttggaatta tcggtactac cttatcccat
    17221 cagctgctat cagaaataaa tttaatgcaa ataaagatat catacttgat gagtatcgat
    17281 ctaatggttt tcagaatttt aatgagaata aaagttttga aaattacttt atcgataatg
    17341 atgttatatt attatcaata ataaatgaag caaaaaaaca gcttaaattg aaagaatctt
    17401 gggataaaga cgcaatcatg ttttgtgata attttggtaa tagtcttacc gtttggccag
    17461 atgatataga gtgcgaactt gatttaagat ttgattatac taaatttatt cagaaaacca
    17521 ttgattgggc aataaaatat aattgtctac ttgtaataga aaaaacagga aatgtagttt
    17581 cccctaatat aaataatctg atgtatgaaa taaaagcata tttggaaagc aagccgtggc
    17641 ccatatgaaa cctaaactca acaagtagga tgtgtgcgga acgcacgtat gcggttctca
    17701 aggtttgagc taagaggccg tctgaaaaca gaaaaactgt ttcagacgac ctttctttta
    17761 accagttgcc acagcaaccg gacaaaagca gcctacctcc acatccatat aggcaataca
    17821 ggggagatat tttgtaaatt ctacgaatat tttacctgct aaacagggta ggatatggta
    17881 tgaagcgaac attggcttaa taaacactat gtcaagatcg aatcaggctg gtactagatt
    17941 gttgtattcc aattatggat tgctatatat aacaactgat cattatatct ctgcaacacg
    18001 gtttgtagct tggaaatagg agtataactt atgcaattag agattatcgg tagtaaaatt
    18061 tatacggaac aagattttca taatcaaatt tcaaaaatat tttctataca agattattat
    18121 gggaacaatc ttgatgcttt atgggattta ttaagcacaa atgtagaacg accgattact
    18181 ttggtatgga aagatgctat gttctcaaaa aatcaattag aaaatatatt tattgaaatc
    18241 gtaaatgttc tagaaagagt taagaaacaa gatgaggatt atggattcga agaaaaattt
    18301 aattatattt tagagtaagt aaaccctaat tacattacgt acacaggctt aaaactcccc
    18361 agagccaatt aagcaagccg taacccatat aaaacttaaa ctcaacaagt agcatgtgtg
    18421 cggaacgtac gcatgcggtt cttaaagttt gagctaagag gccgtctaaa aacagaaaaa
    18481 ccgtttcaga cggtctttgt ttaacgccac cgatccagcg ggttacaaag cgcagtcaat
    18541 gccgctgcgc cttatgcctc cgaagcaata ggcagaacat ttggacacgg tgaaaacaaa
    18601 aacgaaaccg cccaagccgt cggacatttc cttttaggag cagctattgc ccgcgtcaac
    18661 ggtggtaatt ttgctgccgg cggctcggca gcagttgcag ctgaaaaggc ggcggaacat
    18721 cttgcccaac agtataacga cggtaaaacc gcaatcgatc cgcaaacagg cgagttcaat
    18781 gccaacctgc tgccggaaca tatcaaagag gaaatcaaat caaagagcgg ggtgattgca
    18841 tcgctgacgg gcgcggccgt gggcggcacg ccggtagatg cgcaaaccgg aggtgcggtc
    18901 ggacagaatg cggtggaaaa caacctctat ctgacatcgg aagccttaaa gaaggacaag
    18961 cagacagctc gtaaaattta ttccgtcata aaagagcaag tcaagcatga atgcagttcc
    19021 acaggaagaa ttaccgaatg tcgtcaaaat ataggacgca ttatcgaatt tacccaagac
    19081 aaacgctttg acagtaggtt taaggactta aaaaaagaat ccttatatta cctaaataaa
    19141 catcctgatt tagtagcctc ttatttgaag gctgaatacg aaaagctgga tagggaagac
    19201 aaaagtatcc tgcaccgcta catctcaccc ggggctgaaa tcgtttcggg cagtttgggg
    19261 gttgttcttt caggagtagc cggaggcgga tcttgtgccg agactttcgg cttaggctgt
    19321 gccgccgctt tggttggtgt aacgtcttcc tacgatcatg tcattactgg gacgaaaaac
    19381 ttcggaaaaa aagccagcga gcaacgaccg acgattgcgg ttcaggcctt gaagcagttg
    19441 gggctgtcgg agcaggctgc ggaatatgtt cagttctcta tagatttgtt cagtgtgggt
    19501 aaatcggggg gcggtatacc taaggctaag cctgtgtttg atgcgaaacc gagatgggag
    19561 gttgatagga agcttaataa attgacaact cgtgagcagg tggagaaaaa tgttcaggaa
    19621 acgagaagaa ggagtcagag tagtcagttt aaagcccatg cgcaacgaga atgggaaaat
    19681 aaaacagggt tagattttaa tcattttata ggtggtgata tcaataagaa aggcacagta
    19741 acaggagggc atagtctaac ccgtggtgat gtacgggtga tacaacaaac ctcggcacct
    19801 gataaacatg gggtttatca agcgacagtg gaaattaaaa agcctgatgg aagttgggag
    19861 gtgaaaacga aaaaaggtgg gaaagtgatg accaagcaca ccatgttccc aaaagattgg
    19921 gatgaggcta gaattagggc tgaagttact tcggcttggg aaagtagaat aatgcttaag
    19981 gataataaat ggcagggtac aagtaaatcg ggtattaaaa tagaaggatt taccgaacct
    20041 aatagaacag catatcccat ttatgaatag taatatttat gaaaaattag gagattaatg
    20101 atgaaaagaa ttaagtgctt ttgtgataaa tttccatcag gagatacatt tagaatgtgt
    20161 atcattctgg atgactatga taatagggtt gattattatg taggaatata tgattacatt
    20221 acgtctacct taatgagcga tatttactat cgatccacga ttgatgagca tttcaagatt
    20281 atagaattaa tagaaaataa tccaaatgaa atttatgatg atggcggtgg tcaacaattt
    20341 tgcctagaat ttcatcatga taaggtcatt ttttaccaca atgaatttga tgaagaagat
    20401 ggttatccag tattaagctg ttcgctgcat acttttaaaa ctgctttaat tgcttggaat
    20461 gcttttttgc aattgcctaa aagtattcat tcggtggtgg agactgtgat tgaggaataa
    20521 gcataattag cttaatgaat agaatcagcg atatagattg gactgcaaat ccacgcttat
    20581 acgctgtgcc atgattaaca ggaagagagc aacttggatg ggctagtagg gatggtaagc
    20641 atttaaatat atcaattgat ggaaagatta cacacaaatg actaatttaa aattagattt
    20701 ctactctgaa gttattataa aagattcttg ccctaatgat ttgttagaaa atggtgagac
    20761 tattaaagga aaaaaaggag tggtattagg tataagcgaa gaagatggta taatctatgg
    20821 ctatactatt ttactttttg atataaaata ttgtatatat atagataaga aatatattat
    20881 accgactgga aaaaaattct ctcgcgatga tttttattag ctaatttaag taatttaagc
    20941 aagtttttta gatactgaaa ctggtaatta tgtttatgta agcataagga gttcagattg
    21001 aaggattcta cagggcaaat gaggctggca accaaggatt tggcggaagc cattaaacga
    21061 ggagaagtac gtagttctgc ttttacaaca aagcaactaa aggcaatcga aaaaggagaa
    21121 tctaaaatac cggattacac ttggcatcat catcaagaca ctggaaggat gcaattgatt
    21181 aatgaagact tgcatcatga taccggccat atcggttgga gagcgatgag taaaggaaag
    21241 taactatgtg gaaaatcata aaagaggata gtgatgattt aggatttgca attaaatgct
    21301 tattctctca atctattgat ttaaatgaat tcaagttatg gattgaacaa gtaatacgcg
    21361 atatgcccat cgaggacatc cctttttata tttttgactt ggcggatttt gatgggggaa
    21421 ttgccgatat tgacaatatt gtaggttttg tttcgagtta cagcctatca aaatcgaaaa
    21481 aaaatgcctt gacaggcatt gccttcttaa gggggataga tgtctatgat ccgcctattt
    21541 caaaagaaaa agcattaaaa gccttaaaga aacatcctga aatttatcag aagtttcagc
    21601 atttctttcc gtttgtagaa ctgcccccgc tttaaacagt caaaatgccg tctgaaatga
    21661 tattcggctt tcagacggca tttttgacac aaagcaggta accaaaagag cgtttgacgg
    21721 caaaggaaga taattatgtg aaaaattacg aaagagcaag gtcaagattt aagttttgca
    21781 acatattgct tattatcggc agcaattaat ttgcaagaat ttaaattatg gttggagaag
    21841 gttgtcttag atatgcctat tgataatata cctttttata tttttgattt aattgatctt
    21901 aaagaaggag taggagatat ttataatatt ttggattttg ttccgaattc agacttatct
    21961 aaagaccaag atgacgcatt aacaggcatt gccttcttaa gggggataga tgtctatgac
    22021 cctcccgttt caaaagaaaa agcattaaaa gccttaaaga aacatcctga aatttatcag
    22081 aggtttcagc atttctttcc gtttgtagaa ctgcccccgc tttaaacagt caaaataccg
    22141 tctgaaagcc atttccgccg ctcagacggc attttcgccc cttttgttta caaaccctta
    22201 aaatccattt acactcaaac
//
LOCUS       DAWEWP010000016        21704 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWEWP010000016 DAWEWP010000000
VERSION     DAWEWP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.016"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            61..1101
                     /gene="murB"
                     /locus_tag="WGN53_000446"
     CDS             61..1101
                     /gene="murB"
                     /locus_tag="WGN53_000446"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ0139475.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            1113..1763
                     /locus_tag="WGN53_000447"
     CDS             1113..1763
                     /locus_tag="WGN53_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ0139476.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            1818..2594
                     /locus_tag="WGN53_000448"
     CDS             1818..2594
                     /locus_tag="WGN53_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0139477.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSMGKIIRTVCDNSLSL"
     gene            2645..3211
                     /locus_tag="WGN53_000449"
     CDS             2645..3211
                     /locus_tag="WGN53_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ0139478.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            3231..4121
                     /locus_tag="WGN53_000450"
     CDS             3231..4121
                     /locus_tag="WGN53_000450"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ0139479.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            4146..4904
                     /locus_tag="WGN53_000451"
     CDS             4146..4904
                     /locus_tag="WGN53_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0139480.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NRWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(4959..5624)
                     /locus_tag="WGN53_000452"
     CDS             complement(4959..5624)
                     /locus_tag="WGN53_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0139481.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(5737..6633)
                     /locus_tag="WGN53_000453"
     CDS             complement(5737..6633)
                     /locus_tag="WGN53_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ0139482.1"
                     /translation="MQPPKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIQSLLD
                     YDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            6701..7858
                     /locus_tag="WGN53_000454"
     CDS             6701..7858
                     /locus_tag="WGN53_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ0139483.1"
                     /translation="MKFATQAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQVLEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNLDNLDKLLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPMDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(7921..8922)
                     /gene="hemB"
                     /locus_tag="WGN53_000455"
     CDS             complement(7921..8922)
                     /gene="hemB"
                     /locus_tag="WGN53_000455"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ0139484.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGDKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(9105..9389)
                     /gene="yhbY"
                     /locus_tag="WGN53_000456"
     CDS             complement(9105..9389)
                     /gene="yhbY"
                     /locus_tag="WGN53_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ0139485.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            9499..10119
                     /locus_tag="WGN53_000457"
     CDS             9499..10119
                     /locus_tag="WGN53_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ0139486.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFEGLLNNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            10183..12150
                     /gene="ftsH"
                     /locus_tag="WGN53_000458"
     CDS             10183..12150
                     /gene="ftsH"
                     /locus_tag="WGN53_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ0139487.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQIA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            12371..12802
                     /locus_tag="WGN53_000459"
     CDS             12371..12802
                     /locus_tag="WGN53_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ0139488.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSVTLVEAFVKEADRRYA"
     gene            12795..13073
                     /locus_tag="WGN53_000460"
     CDS             12795..13073
                     /locus_tag="WGN53_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ0139489.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            13126..13704
                     /gene="pth"
                     /locus_tag="WGN53_000461"
     CDS             13126..13704
                     /gene="pth"
                     /locus_tag="WGN53_000461"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ0139490.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            13765..13971
                     /locus_tag="WGN53_000462"
     CDS             13765..13971
                     /locus_tag="WGN53_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ0139491.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            13968..14330
                     /locus_tag="WGN53_000463"
     CDS             13968..14330
                     /locus_tag="WGN53_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ0139492.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     LTGALLALFAARSADRPD"
     gene            14561..15976
                     /locus_tag="WGN53_000464"
     CDS             14561..15976
                     /locus_tag="WGN53_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ0139493.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVALLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSSAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            16095..16604
                     /locus_tag="WGN53_000465"
     CDS             16095..16604
                     /locus_tag="WGN53_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0139494.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            16767..18149
                     /locus_tag="WGN53_000466"
     CDS             16767..18149
                     /locus_tag="WGN53_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ0139495.1"
                     /translation="MANIAHDIFKAYDIRGIVGKTLTDEAAYLIGKAIATKASEKGIT
                     RIALGRDGRLSGPGLMAQIQHGFTDSSIDVLNVGMIATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGEYHNNI
                     VGHIKLKRPMKIVIDAGNGVGGAFAGKLYKGLGNEVAELFCEVDGNFPNHHPDPSKPK
                     NLQDLIAALKNSDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLTPWIKEHGGEPVMEKTGHSFIKSAMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(18169..18924)
                     /locus_tag="WGN53_000467"
     CDS             complement(18169..18924)
                     /locus_tag="WGN53_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0139496.1"
                     /translation="MIKIRNIHKTFGESTILRGINLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(18934..19650)
                     /locus_tag="WGN53_000468"
     CDS             complement(18934..19650)
                     /locus_tag="WGN53_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0139497.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(19640..20467)
                     /locus_tag="WGN53_000469"
     CDS             complement(19640..20467)
                     /locus_tag="WGN53_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0139498.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            20752..21660
                     /locus_tag="WGN53_000470"
     CDS             20752..21660
                     /locus_tag="WGN53_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ0139499.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT         5351 a         6388 c         5356 g         4609 t
ORIGIN      
        1 actatagaaa cgatgggctg aagcccgcca ctggcaatac tcattttaaa gttcaaaccc
       61 atgcaaccca tccgataccg aaccgacctt accccctaca acactttcgg cctgcgcgcc
      121 caagcccgag cctttattgc gcttgaacac gccgacgggt tgcgcgacat cgtccggcta
      181 cccgagttca accgagatac tgttttatgg ctgggcggcg gcagcaatat ccttttgatg
      241 gaggactacg ccggtctggt cgtacatatg gaaaacaaag gcatacgcga aattgctcgt
      301 tcagacggca tggttctgat tgaagcgcag gcgggcgaaa tctggcacga ttttgtcctg
      361 cacgccgtcg cgctgggttt gaacggtttg gaaaacctga gcctgattcc gggtacggtc
      421 ggcgcgtcgc ccgtgcagaa catcggcgca tacggcgtag aggcgaaaga tgtgattcac
      481 agcgtgcgct gcttcgattt ggatacggaa acctttgtaa ccctttccaa tgccgactgc
      541 cgcttcgcct accgcgaaag cctgttcaag caggaaggta aagggcgtta tgtgattgtt
      601 tcggtcgtat ttgcattaaa aacgcatttt gtgccgaact tgggttacgg cgatttggcg
      661 gccgccgttg ccgaactgag cgcgggcagg gtgccgacgg cgaaagatgt ttccgatgca
      721 gtgtgtgcaa tccgcaacag taaacttcct aatcctaacg tgcttggcaa tgtcggcagt
      781 ttctttaaaa accccgtcgt cagcgcagaa aaagccgcca ccttgttgca gcggcatcct
      841 gatatgccgc gctatccgca gcccgacggt tcggtcaaac tcgccgccgg ctggctgatc
      901 gaccaatgtc gtctgaaagg ctttcaaatc ggcggcgcgg cggtacatga caggcaggct
      961 ttggtcttag tgaacaaaaa caatgcctcg gcaaacgatg tccggcagtt ggcgcaacac
     1021 atcaaattta cagtatttgc tcggtttcag gtagaattgc acgccgaacc caattggctg
     1081 cctgcttcgt tcagcctgta aatccaagga gtatgcccgt gacccgcccc gccaaaatca
     1141 atacttacac acgcatcatc gacgccagcc ttgcgctttt caacgaggaa ggcgagcgca
     1201 acatcagcac caaccatatt gccgcccact tgggcatcag tccgggcaac ctctattacc
     1261 acttccgcaa caaagacgaa atcatcgtcc aactgttcaa acgttacagc gaagccctgc
     1321 tggaatacct caacgaagcc gtgttgccgt ctgatgtgga agactccatc aattatatgg
     1381 cgggcattta cgacgtgatg tgggaatacc gcttcctatt cagcgacgtg aacaccctgc
     1441 ttgcacgcag tgccgaacta ttgggcgaac acaatacctt cacccaagcc aaagtctccc
     1501 cgctcttggt caacctgctg acccagctca acggtctgaa catcatccaa gccgaccaaa
     1561 ccgccatgaa cgacctcgcc gtcaatatgt ggatgatcac gaaatactgg ttcgacttcg
     1621 acagctccct gcgcggccgc accaagctga ccgaagactc caaagcacgc ggcatccgcc
     1681 gcaccttaag cctccttcgt ccttatcttt tgcccgaaca ccgcgagaaa tacgaccgga
     1741 aaatcggcaa cggcaacccg taaacccaca gcgtccgaat cagccttcag acggcactta
     1801 cccgacggta tcagcgaatg cgcgcagtcc ccccgcccca tatctccatc acaggcctag
     1861 gttacctcgg cctgccgctg gcacaaaagt tttaccaaca cggcagccgc gtcgccgcca
     1921 taaaacgcag cctgacttcg gacgacatca acctgcccat acatctcgac accttcgacc
     1981 tcggcagcac cgatgccttt cagacggcat tgtggcggca ccacgccgac aaacccgtct
     2041 ggttcttcct gctaccgccc tcctcgctga cgcattacgc cgacaccgtc aaacaatggg
     2101 cagaacttgc ccgggcgtgc aacgtgcaac acctgatttt cacaagcagc accagcgttt
     2161 acggcgatac agcgcgcgaa tgcgacgaaa ccgccacacc cgatccgcaa accgagtccg
     2221 cccgccaaat cctcgccgcc gaacaacacc tgctcgacag cggcatctcc aacatcgaca
     2281 tcctgcggct gggcgggctt tattgcgccg aacgccatcc cgtcagccgc cttgttcaaa
     2341 agcaaaacat cccgggcggc aaccgcccca tcaacatcgt ccaccgtaat atcgccgtcg
     2401 aaagcctgtt tcagacggca tttaaccccg gcggcaggcg gctgaaaaac attatcgaac
     2461 cgcgccaccc gacacgacgc gaattctaca cggaagaagc cgccaaactc ggtttgccgg
     2521 ctcctgattt cgcacccgac gacagcatgg gcaaaatcat ccgtaccgtt tgcgataaca
     2581 gcttaagcct gtaaaataag tgtcaacagc aacaaacagg cattctccgc cccgatcaga
     2641 aaatatgtcc gccctcctcc ccatcatcaa ccgcctgatt ctgcaaagcc cggacagccg
     2701 ctcggaactt gccgcctttg caggcaaaac actgaccctg aacattgccg ggctgaaact
     2761 ggcgggacgc atcacggaag acggtttgct ctcggcggga aacggctttg cagacaccga
     2821 aattaccttc cgcaacagcg cggtacagaa aatcctccaa ggcggcgaac ccggggcggg
     2881 cgacatcggg ctcgaaggcg acctcatcct cggcatcgcg gtactgtccc tgctcggcag
     2941 cctgcgttcc cgcgcatcgg acgaattggc acggattttc ggcacgcagg cagacatcgg
     3001 cagccgtgcc gccgacatcg gacacggcat caaacaaatc ggcaggaaca tcgccgaaca
     3061 aatcggcgga ttttcccgcg aatccgagtc cgcaaacatc ggcaacgaag cccttgccga
     3121 ctgcctcgac gaaataagca gactgcgcga cggcgtggaa cgcctcaacg aacgcctcga
     3181 ccggctcgaa cgcgacattt ggatagacta accttcagac ggcatccgac atgaacagcc
     3241 cttttcacaa catcggcatc gtaacccgcc ccaacacgcc cgacatccaa gacaccgcac
     3301 acacgctgat tacctttttg aagcagcacg gctttaccgt ctatctcgac gaagtcggca
     3361 taaaggaagg ctgcatctat acccaagaca ccgtcggctg ccatatcgtc aacaagaccg
     3421 aactggggca atactgcgac ctggtcgccg ttttaggcgg agacggcacc tttctctccg
     3481 tcgcccgcga aatcgccctg cgcgccgttc cgattatcgg catcaaccaa gggcatttgg
     3541 gcttcctgac ccaaattccc cgcgaatata tgacggacaa gctattgccc gttttagaag
     3601 ggaaatacct tgccgaagag cgcatcctga ttgaggccgc actcatccgc gaaggcaaaa
     3661 ccgccgaacg cgccatcgcc ctcaacgatg ccgtcctctc ccgtggcggt gccggacaga
     3721 tgattgagtt tgaagtcttc gtcaatcggg aattcgtcta tacccagcgt tcggacgggc
     3781 tgattgtctc cacccccacc ggatcgaccg cctattcgct tgccgccggc ggccccatca
     3841 tgcaggcagg attacacgcc ttcacgctcg tccccatctg cccgcaatcc atgaccaacc
     3901 gccccatcgc cattccagac acgtccgaaa tcgaaatcct cgttacccaa ggcggcgacg
     3961 cgcgcgtcca tttcgacggt caaacccata tcgacgtgca aaacctcgac cgcatcacca
     4021 tccgccgcta ccgaaatccc ttacgcattt tgcacccgac cgactaccaa tatttcaaaa
     4081 ccctgcgcca aaaactgcac tggggcgagc aattagtcta agccggcctt tacccgaaag
     4141 tatccatgaa tcccaaaatt tcctccgacc atacagaaga tgccgtgcgc ctctccaaac
     4201 gcatggcgca actggggctt tgttcgcgcc gcgaagccga cggctacatc gaacagggct
     4261 gggtaacggt caacggcaaa accgccgtac tcggtcagaa agtttcaccg gccgaccgta
     4321 tcgaactgaa caagaaagcc cacgaacagc aggcggcacg cattaccatc ctgctgaaca
     4381 aacccgtcgg ctatgtcagc gcacaagcgg aaaaaggcta taaatccgcc gccgaactga
     4441 ttacccctga aaaccgctgg gaaggcgaca ccggccgcat ccgtttcgat ccgaaacaca
     4501 aaatcggcct cgcccccgcc ggcaggctcg atatcgattc agtcggatta ctggtattga
     4561 cgcaggacgg ccgtatcgcc aagcagctta tcggcgaaaa cagcggcagt gaaaaagaat
     4621 atttggtgcg cgtgcgcggc aaattggacg aaaaaggact tgccttactg aatcacggat
     4681 tgagtttgga cggcgagaaa ctgcgtcccg cccaagtaga atggcaaaac gaagaccaac
     4741 tgcgcttcgt gttgaaacag ggtaaaaagc ggcaaatccg ccgtatgtgc gaactggtcg
     4801 gactgcgcgt cgtcgggctg aaacgcatcc gcatgggcaa ggtcaaactc ggcaggctgc
     4861 cgcccggcaa atggcgttat cttgctcccg gcgaatcgtt ttaaataagc attaaaaaat
     4921 gccgtctgaa aatccttgcg ttttcagacg gcattgcctt atgcccaaat aacggtttct
     4981 tccaagggcc tgcgcgcctt cgtggcgatt tcctgaacgc ggtaaccgaa tgtcgcggcg
     5041 acggacacgc cccattctgc cgcatcgaac aaaccgaact gcccggacaa tacgcgttcc
     5101 atatcggcat agttgaaacc ttccaccggg caggaatcga tacctgccat cgccgcaccc
     5161 gtcatcatgt tgcccaacgc gatataggtc tggcggcaac accagtcaaa caaggcgcgc
     5221 gaatcgtcca aaatcttgat gtcgtcggct tgaaacgcct gatacctcgc caaagatttt
     5281 gccatggcat ccggttcggt aacgccgcgc cgtttgaggc tttccaacat aaacgggctg
     5341 tcgaagcggg catttttctt tgccaaaaac accaccaaat gactggcggt atccaaagca
     5401 tccgccatac cccaagaaaa cggcttgatt gcctgtcgga tttcagggtt ttgaaccaca
     5461 acaaactgcc aaggctccga accgaccgaa ctgggcgaca aacgcccgag ttctaaaata
     5521 aactgaaaat cctcggcact gattttacgt gccgcatcgt aatgccggca tgatttgcgg
     5581 tttttaaatg cggatagaac ctgctctctg tctaataccg tcatagtgca tcctttatta
     5641 cttttattgc aaattgattt caaaccgata cggcggcaga ttcgccaata acgcccaaac
     5701 gcctttcaga cggcatttgg ttcatcgcaa acacgcctac gggtaggcga tataggcata
     5761 agccgaatgg ttgtggatag attcgaaatt ctcactctcg acgacgaaac tcttgatgcg
     5821 tttgtcggca atcagcgaag tagcgacatc gcgcaccata tcttccacga atttcgggtt
     5881 ttcgtaggcc ttttcggtaa cgtatttttc atcggggcgt ttgagcaggc cgtagagttg
     5941 gcagctcgcc tgcgcctcca cataatcgat gacttcctcg ataccgactt cggcatcggc
     6001 agtcaggctg acggtaacgt gcgaacgctg gttgtgcgcg ccgtattggg aaatttcttt
     6061 ggaacacggg caaagcgagg ttacgggaat catgaccttc atactgtggc cgtatgcacc
     6121 gtctttcatt tcgcccgtga ggctgacatc ataatccagt aaagactgaa taccggaaac
     6181 cggggcggtt ttcttgcgga aaaatgggaa agaaacgctg attttgccgg cgcgggaatc
     6241 caaaagcgcg accatttcag cagtcagcct gcgcaattgt gcaaaatcca aggcttcggc
     6301 atgttgctcc atcaatgcga caaagcgcga catatgcgta cccttctgct cggcgggcag
     6361 gtaaaccgtc atggtcagac gggcaactgt ggactggatg ccttctgcag tttgcagggt
     6421 aatcggaaag cgcaggtctt tgataccgac ctgattgatc ggcagattgc gcaaatctcg
     6481 gctggattgc acgtctgcaa tagtgttcat gagttgtttt tccttaatat tcatgtaatt
     6541 gttgagatgt gtgcatggta tggcgtttcc gccggggaat ataccgtcaa tctgcaaatg
     6601 cgagattata tcacttgctt ttggcggctg cattgattgt ttctattttc gggatagtga
     6661 taaaatcccg acttattcat atcttacaag gcagaaacag atgaaattcg ccacccaagc
     6721 catccattcc ggctacgatt gcgacgaaca caaccgcgcg ctgatgccgc cgatttatca
     6781 aaacagtatg tttgcgctgc acgaaatcgg cgaaaatgtg ccttaccgct attcacgtct
     6841 gagcaacccg acccgtcagg tattggaaga taccgttgcc gatttggaac acggtgcggc
     6901 aggttttgcg ttttccagcg gtatggcagg gattgatgcc gtatggcgca ccttcctgca
     6961 accgggcgat accattgtcg ccgtcgccga tatttacggc ggcgcgtatg atttattggt
     7021 cgatgtttat caaaaatggg gggtgaacgt tgtttttgcc gatttgggca atctggataa
     7081 tttggacaag ctgcttaaag cgcacaaggt caaactggtt tggctggaaa cgccgtccaa
     7141 tccgcttttg cgcttggtag acatcaaagc gcttgccgcg aaagccaaag cggccggcgc
     7201 actggtcggc atcgacaaca cctttgccac gccgtatctg caacagccct tggatatggg
     7261 ttgcgatttt gtgttccatt ccgctaccaa atatttgtgc ggccattccg acgtgttgat
     7321 gggcatcgtc gttgccaaaa ccaaagagct ggcgcagcct ttgcacgata tgatggtgca
     7381 taccggcgcg gttgccggcc cgatggactg ctggctggtg ttgcgcggca tcaaaacact
     7441 ggctctgcgc atgaacgccc attgccaaaa cgcactcgaa atcgcgcgcc gtttggaagc
     7501 ccatcctgcc attgaaaaag tgttccatcc cggcctgccg tctcacgaac attatgaact
     7561 cgcccaagcg caaatgccta aaggcatcgg cggcgtggtg acggtttatc tcaaaaacga
     7621 cacgcgcgaa gcggcaaaca gcgtgattaa aaacatgaaa ctggtcaaaa tggcttccag
     7681 cctcggcggc gtggaaagtt tggtcaacca ttgctattcc cagtcccaca gcggcgtgcc
     7741 gcataatgtg aaaatggaaa tgggcatcaa agtcggcctg ctgcgtttct ccatcggcat
     7801 cgaagacgcg gacgatattt ggaacgatat ttccgccgca ctcgatacaa ctttgtaaac
     7861 cgtaaaaatg ccgtctgaaa ccatagtttc agacagcatt tcaatattgc gaatcaaata
     7921 tcaacgcttc agcatcttgg ccgcctcaat ggcataatag gtcaaaatcc catccgcgcc
     7981 cgcacgtttg aatgccagca ggctttccaa aaccacttta tcgccgtcca gccatccgtt
     8041 ggcaaccgcc gcctgcagca tcgcatattc gcccgaaacc tgataggcat aagtcggcac
     8101 accgaactcg tccttcacgc ggcggacaac gtccaaatac ggcaaaccgg gcttcaccat
     8161 caccatatcc gcaccttcct gaatatcgag cgctacttca tgcagcgcct catcggtgtt
     8221 tgcgggatcc atctgatagg tctttttatc tgccttgccc aaattgcccg aactgcctac
     8281 cgcatcacgg aaagggccat agaatgcaga agcatatttg gcggaatatg ccataatccg
     8341 cgtgtggata tgtccggcat cctccaacgc ctcgcgaatc gcaccgatac gcccgtccat
     8401 catatccgac ggagcaacca cctgcgcgcc cgcttcagcg tggcacaaag cctgcttgac
     8461 caaaacctct acggtttcat cgttcatcac ataaccgttt tcgtccgtca gcccgtcctg
     8521 accgtgaacc gtataaggat cgagcgcgac atccgtcata atgcccagtt cgggaaacct
     8581 ctcgcgcaag gcgcggacag ttgacggcac gagtccttcg ggattgtacg cctcctgcgc
     8641 acgctcggtt ttgtttgccg taaccacggg gaacagtgcc aacatcggaa taccgagctt
     8701 taccgcctct tccgccgtaa acagcagcct gtccaaactt tgacgcttca cacccggcat
     8761 agaaggcaca tcctcctcgc gcgccgaccc ctccaatacg aacaccggat aaatcaaatc
     8821 atcggcggtc agcgtgtgtt cgcgcatcag gcggcgtgaa aaatcgtccc tgcgcatacg
     8881 gcgcatacgc gaagccggaa cattgcggta aggaaactgc ataagccctc caatcatcct
     8941 gtcaacaatt caaaccatac ggaagccgcc gcaacttgca gacagcatcg ggcggcaatc
     9001 aaaatccgtt ttcaccggaa agggtttcgg cccgccgaaa tgccgcgccg aaggacggcg
     9061 tcccgcccat taaagaacaa tagcaaaaca acagcttcag gctgtcagcc ttcgatattc
     9121 ttacgccaca ataccaaaag tttcccgata tgctgaacag gttgcgcgtc aaccgcctca
     9181 cataaggcag tgcagatttc gatacgttcg gcacggtcgt cgccgaatac gcgcacttta
     9241 atcagctcat gcgccgtcaa tgccgcatcg gtttccttga tgaccgcgtc cgtcagaccc
     9301 tgctgaccga ccatcacaac aggatggaga tggtgcgcgc gcgctttcag ttccaaaatt
     9361 tctttggtgt tcaatttggt atccgtcatt ttcctactgc ttgaataaaa agaataatgc
     9421 ggcattgtac gcgatttggc acgacatcgg caaaattaac gtacaatacg cggtttccat
     9481 tccgacccgc gaaccactat ggctgtacgt tccaaatcct caaaagcgtg gctgcacgaa
     9541 cacgtcaacg accactacgt ccatatggcg caaaaagacg gctaccgcgc ccgtgccgca
     9601 tacaaacttt tggaaatcaa cgaaaaagac aaattaatca aacccggcac ggtacttgcc
     9661 gacttgggca gcgcaccggg aagctggtcg caggttgccg ccaagctgac gggtacttcc
     9721 ggagcagttt tcgccttgga catcctgcct atggaagcca tagggggcgt ctccttcatt
     9781 cagggcgact tcagggaaaa cgacgttttg gcgcaatttg aaggcttatt aaacaaccgc
     9841 cccttagacc ttgtaatctg cgatatggcg cccaatatgt cgggaaacgc cgtaagcgat
     9901 caggcgcgca gcttttattt gtgcgaactg gctttggact tcgcctcgca acacctgaaa
     9961 accggcggca gctttttggt caaagtcttt cagggtgcag gctatcagga atacatggca
    10021 gccatgcgcg aaattttcgg cacggtgcag acgcgcaaac ccgaagcctc gcgcaatcgc
    10081 tccagtgaga tttatttatt gggcaaaaat aaacgctgac aatacagacg gcgtgcttta
    10141 caatcatttc cgttttacac ctcaattatg gagccttgct aagtggggaa cacctttaaa
    10201 tcaatccttg tctgggtcgc cttgggtatc ggcctgatgg ctgcgttcaa cgctttagac
    10261 ggtaaaaaag aagacaacgg gcaaatcgaa tattctcagt tcatccaaca ggtcaacaac
    10321 ggcgaagtat ccggcgtcaa catcgaagga tccgtcgtca gcggctacct gattaagggc
    10381 gagcgcaccg acaaaagcac cttcttcacc aacgcgcctt tggacgacaa cctgattaaa
    10441 acactgctcg acaaaaacgt ccgtgtaaaa gtaacgccgg aagaaaaacc gagcgcgctg
    10501 gctgccctgt tttacagcct gctgcccgtc ctgctgctga ttggcgcgtg gttctacttt
    10561 atgcgtatgc agacgggcgg cggcggaaaa ggcggcgcat tctcattcgg caaaagccgc
    10621 gcccgcctac tggacaaaga tgccaacaaa gtgacctttg ccgatgtcgc cggctgcgac
    10681 gaagccaaag aagaagtgca ggaaatcgtc gattacctca aagcgccgaa ccgctatcaa
    10741 agcctgggcg ggcgcgtgcc gcgcggcatc ctgctggcgg gcagcccggg tacgggtaag
    10801 acgcttttgg cgaaagcgat tgcaggcgaa gccggcgtgc cgttcttcag catttcaggt
    10861 tccgactttg tcgaaatgtt cgtcggtgtc ggtgcaagcc gcgtccgcga tatgttcgag
    10921 caggcgaaga aaaacgcccc ctgcatcatc tttatcgacg agattgacgc agtcggccgc
    10981 caacgcggcg caggtttggg cggcggcaat gatgagcgcg agcaaacatt aaaccaattg
    11041 ttggttgaaa tggacggttt tgagagcaat cagactgtaa ttgtgattgc ggcaaccaac
    11101 cgccccgacg tactcgatcc tgcgctgcaa cgccccggcc gtttcgaccg ccaagtcgtc
    11161 gtccccctgc cggacatccg ggggcgcgaa cagattttga acgtccattc taaaaaagtg
    11221 cctttggacg aatctgtgga tttattgtcc ctcgcgcgcg gcacgccggg tttttccggt
    11281 gcggatttgg caaacctggt taacgaagcc gccctgtttg ccggccgccg caacaaagtc
    11341 aaagtcgatc aaagcgattt tgaagacgca aaagacaaaa tctatatggg tccggaacgc
    11401 cgcagtatgg tgatgcacga agacgaaaaa cgtgcgacgg cgtatcatga gtccggacac
    11461 gcgattgttg ccgaaagcct gccctttacc gaccccgtcc acaaagtaac cattatgccg
    11521 cgcggacgtg cgctgggtct gacttggcag cttccggagc gcgaccgcat cagtatgtat
    11581 aaagatcaga tgttgagcca gctctccatc ctgttcggcg gacggattgc cgaagacatc
    11641 ttcgtcggac gcatctccac cggcgcatca aacgactttg aacgcgcaac ccaaatggcg
    11701 cgcgaaatgg taacgcgcta cggcatgagc gacaaaatgg gcgtgatggt ttatgcggaa
    11761 aacgaaggcg aagtcttctt aggacgcagc gtaacccgtt cgcaaaacat ttccgagaaa
    11821 acccagcagg acatcgatgc ggaaatccgc cggattttgg acgagcagta tcagattgcc
    11881 tacaaaatcc tcgatgaaaa ccgcgacaag atggaaacca tgtgcaaagc cctgatggaa
    11941 tgggaaacca tcgaccgcga tcaggtactg gaaatcatgg cgggcaaaca acccagtccg
    12001 cccaaggatt acagccacaa cctgcgcgag aatgcggacg cggcggaaga taacgcgccg
    12061 cacgctccga ctcgggaaga aaccgaagca cctgccccgg cagacaccgc ttcgacagag
    12121 tccgagcagc agcctgaaaa caaggcttaa cttcccgaac aaacggcagc ccgcaagctg
    12181 ccgttttccc atcctgtttt cagacggcat tttccgctca atgccgtctg aaacgccaaa
    12241 gccgtatcgg tttttgcgga tctgcgatat ggcggtttaa attgaaatca gacgaggcaa
    12301 ggctgtaccg gtttaaaatt aaaccgctat aataccgccc ttcgaataca cgcccgcatt
    12361 ggtgatgcct gtgaaaaaag ttgaaaaaaa catcttggtg ctgcacggcg cggacaaaat
    12421 gttcgagctg gtcgataagg ttgaagacta tccgcacttt ctgccgtggt acagcaagac
    12481 cgaggtcatc gggcgtagcg gcaacgaact gaaggcgcgg ctgtttatgg actatatgca
    12541 cgttcgccaa tcattcgcca cgcacaaccg caacattccg ggcagggaaa tccgtatgga
    12601 actgctcgag ggtccgttca aaaccttacg tggaacgtgg aaatttatcg atttgggcga
    12661 cgatatgtgc aaaatcgaat tcaatttgga atacgatttt tccaatgccg ttttgtccgc
    12721 cttaatttcc cccgtcttca accacctttc cgtcacgctg gtcgaagcgt tcgtcaaaga
    12781 ggcagaccgc cgttatgctt gaaattgaga ttgtgtacgg gctgcccgac cgacaggttt
    12841 tgaaaaccat gcagcttgcc gaaggaacaa ccgtccgcgc cgccgcactg caaagcggtt
    12901 tggacggcat atttgaagat ttaaacctgc attccgcgcc tttgggcatt ttcggcaaag
    12961 ccgtcaaaga cgacacgccg ctgcgcgacg gcgaccgcat cgaagtgtac cgcccgctgt
    13021 tgatcgaccc caaagaagcg cgccgcaaac gcgttcaaaa tcaagaagaa taaccatgcc
    13081 gtctgaagcc ttcagacagc attcgacaga aacacggaaa atatcatgtc aaacacaatc
    13141 aaaatggttg tcggcttggg caacccgggc aaagaatacg aacagacacg ccacaatgcg
    13201 ggtttttggt tcctcgacga actggcgtgg aaatggaagg cttcatttaa agaagaaaaa
    13261 aaattcttcg gcgaagtcgc ccgtgccgcc ctgcccgacg gcgacgtttg gctgctcaaa
    13321 cctgccacgt tcatgaaccg ttccggacag gcagttgccg cccttgcgca gttttataaa
    13381 atcaaacccg aagaaatcct cgtcgtccac gacgaactcg acattccctg cggacggatc
    13441 aaattcaaac tcggcggcgg caacggcgga cacaacggct tgaaagacat tcaggcaaaa
    13501 ctcggcacgg cagactatta ccgtctgcgc ctcggcatcg gccacccggg cgaccgcaac
    13561 ctcgtcgtcg gctatgtcct gaacaaaccc agtacggaac accgccgaca gattgacgat
    13621 gccgtcgcca aatccctgca ggccataccc gacatccttg ccggcaaatg ggaagaggca
    13681 acccgcttcc tgcacagcaa atgacccgat gccgtctgaa gccctttcag acggcatgtt
    13741 cccgatttcc atatccgaac agtcatgacc gaactcaagc agcttatcca aaccgaatcc
    13801 atccccgtca tcgaagaaac cctcgatttc ctgctgtacg aatgcagcat cgacgacgca
    13861 ccatccgccg aagaagtggc acaatggcgc gacatacttg ccgcacgcgg cggcaaattc
    13921 ctgcgcctgt ccaaaatctg ccaaacgtgg ctggacgagg aggcggcatg aagctgccgc
    13981 gcaaccgctt cagcctgctt tccgcattgt ggtttgccgg cggcatctat tcgctgctct
    14041 tcaaagctgc cgacaccgcg ccgccgccgt ttccgcattt cgacaaagca gcacaccttg
    14101 ccctgttttt cgcacaaatc tggcttttga ccaaagcatt caaaaccgga aaacttccca
    14161 tcccctaccg cagcctgatg gtctttgccc tctgtttcgc cctcttcagc gaatgcgcgc
    14221 aggcatggtt taccgcaacg agaaccggca gtttgggcga tgtccttgcc gacctgacgg
    14281 gtgcattgct cgcactcttt gccgcacgtt ccgccgaccg cccggactga atcggtttat
    14341 tttccccaaa caggatgcac ctcttcccga tacatcctat ttttcccctt acatatcaat
    14401 ctcatctcat aaacaaaacc gacaaatcgt ttacaatata tttacactac atcagattac
    14461 aaatatactc gaaccagttg cagaagcggc gattccacaa accgtttcgg attgcacgac
    14521 acaaaagaaa aaccgatttt gttgccttaa aggagtattc atgaacctgc atgcaaagga
    14581 caaaacccag catcccgaaa acgtcgagct gctcagtgcg cagaagccga ttaccgactt
    14641 taagggcctg ctgaccacca ttatttccgc cgtcgtctgt ttcggcattt accacatcct
    14701 gccttacagc cccgatgcca ataaaggtat cgcgttgctg attttcgttg ccgcactttg
    14761 gtttaccgaa gccgtccaca ttaccgtaac cgcactgatg gtgccgattc tcgccgtcgt
    14821 actcggtttc cccgacatgg acatcaaaaa ggcgatggct gatttttcca acccgattat
    14881 ctacattttt ttcggcggct tcgcgcttgc caccgccctg catatgcagc ggctggaccg
    14941 taaaatcgcc gtcgccctgc tgcggctgtc gcgcggcagt atgaaggccg ccgtgctgat
    15001 gttgttcctc gttaccgcct ttctgtccat gtggatcagc aacaccgcca ccgccgcgat
    15061 gatgctgcct ctggcaatgg gtatgctgag ccacctcgac cgggaaaaag aacgcaaaac
    15121 ctacgtcttc gtcctgctcg gcatcgccta ttgcgccagc atcggcggct tgggcacgct
    15181 cgtcggctcg ccgcccaacc tgattgccgc caaagcccta aatctggact tcgtcggctg
    15241 gatgaagctc ggcctgccga tgatgctgtt gattctgccc ttgatgctgc tctccctgta
    15301 cgtcatcctc aaacctaatt tgaacgaacg cgtggaaatc aaagccgaat ccatcccttg
    15361 gacgctgcac cgcgtgatcg cgctgttgat tttccttgcc acagccgccg cgtggatatt
    15421 cagctccaaa atcaaaaccg ccttcggcat ttccaatccc gacaccgtta tcgccctgag
    15481 tgccgccgtc gccgtcgtcg tcttcggcgt ggcgcaatgg aaggaagtcg cccgcaatac
    15541 cgactggggc gtgttgatgc tcttcggcgg cggcatcagc ctgagcacgc tgttgaaaac
    15601 atccggcgcg tccgaagcct tgggacagca ggttgccgcc accttttcca gcgcgcccgc
    15661 atttttggtg atactcatcg tcgccgcctt cattattttt ctgaccgagt tcaccagcaa
    15721 caccgcctcc gccgcattgc ttgtaccgat tttctccggc atcgctatgc agatggggct
    15781 gcccgaacaa gtcttggtat tcgtcatcgg catcggcgca tcttgtgcct tcatgctgcc
    15841 ggttgccaca ccgcctaacg cgattgtgtt cggcacgggc ttaatcaagc aacgcgaaat
    15901 gatgaatgtc ggcatactgc tgaacatcct ctgcgtagta ttggttgctc tgtgggctta
    15961 tgctgtactg atgtaaacca tcgacctaaa caacaagacc gtctgaaaga atatttttca
    16021 gacggccttg aagtttgtcg ttcagacaca atttgtcgaa tcattcaaaa ccggattcta
    16081 acgaaaggaa acccatgatt atcctgcaca ccaacaaagg cgacatcaaa atcgaactcg
    16141 atttcgacaa agcccccgtt accgccaaaa acttcgagca atacgtcaaa gacggcttct
    16201 acgacggcgt aatcttccac cgcgtcatca aaggcttcat gattcaaggc ggcggcatgg
    16261 atgaaaacat gaacgaaaaa gaaacccgcg atccgattca aaacgaagcg tccaacggcc
    16321 tgcccaacga taaatacacc atcgccatgg cacgcacttc cgacccccat tccgccagcg
    16381 cgcaattctt catcaacact gccgacaacg ctttcctgaa cttccgttct aaagagctgt
    16441 acggcaaaac cgtcgtccaa gactggggct atgccgtatt cggtaaagtc gttgacggtt
    16501 ttgacgttgt cgatgccatc gaaggcgtat ccaccaaacg ccacggctac catgacgacg
    16561 taccgagcga acctgtcgtc atcattaaag ccgaagcggt ataaaccggc aatccgaaag
    16621 cagcctgcac aaaggctgct ttttttgttt caggcggcat aaaatccaag cacatatgcc
    16681 gtctgaacac ctcccgcaag ccttcggaca atgttgcaac gtgttaaaat agcctccttt
    16741 cctgaaaccg aaacgaggga acccctatgg caaatatcgc ccacgacatt ttcaaagcct
    16801 acgacatccg aggcatcgtc ggtaaaaccc tgaccgacga agctgcctat ttaatcggca
    16861 aagccattgc caccaaagcc tccgaaaaag gcattacccg catcgccctc ggccgcgacg
    16921 gccgtttgag cggccccggc ctgatggcac aaattcaaca cggttttaca gacagcagca
    16981 tcgacgtcct caacgtcggc atgattgcca ctcctatgct ctacttcgca gccatcaacg
    17041 aatgcggcgg cagcggcgtg atgattaccg gcagccacaa tccgcccgat tacaacggtt
    17101 tcaaaatgat gctcggcggc gacacgctcg caggcgaagc cattcaagaa cttttagcta
    17161 ttgttgagaa agacggtttt gttgccgccg acaaacaagg cagcgtaacc gaaaaagaca
    17221 tctccggcga ataccacaac aacatcgtcg gccacattaa gctcaaacgt ccgatgaaaa
    17281 tcgtgattga cgcgggcaac ggtgtcggcg gcgcgtttgc cggcaaactc tacaaaggtt
    17341 tgggcaatga agtggccgaa cttttctgcg aagtggacgg caattttcct aatcaccacc
    17401 ctgatccttc caaacccaaa aacctgcaag atttgattgc cgcgctgaaa aacagcgatg
    17461 ccgaaatcgg cttggctttt gacggcgatg ccgaccgctt gggcgtggtc accaaagacg
    17521 gcaacattat ttatcccgac cgccaattaa tgctgttcgc ccaagacgtg ttgagccgca
    17581 atcctaaagc aaaagtgatt ttcgatgtca aatccacccg cctgctgacc ccgtggatta
    17641 aagaacacgg cggcgaacct gtgatggaaa aaaccggcca cagctttatc aaatccgcca
    17701 tgaaaaaaac cggcgcgctg gttgccggcg aaatgagcgg acacatcttc ttcaaagaac
    17761 gctggttcgg cttcgacgac ggtctgtacg ccggcgcacg cctcttggaa atcctgtctg
    17821 cctccgataa tccgtccgaa gtgttaaaca acctgccgca aagcatttcc acgcccgaac
    17881 tcaacatcgc cctgcccgaa ggcagcaacg gccatcaggt tatcgacgaa ctcgccgcca
    17941 aagccgaatt tgaaggcgca accgaaatca tcaccatcga cggtctgcgc gttgaatttc
    18001 ccgacggctt cggtctgatg cgtgcttcca ataccacgcc gattctggtg ttgcgttttg
    18061 aggcggatac gcaagaagcc atcgagcgga ttcaaaacca attcaaagcc gtcatcgaaa
    18121 gcaatccgaa tctaatctgg cctctgtaaa cacaggaaaa atgcttaatc aaatcttggt
    18181 agattggatt tggcttaaaa atctccgcgt ccgttcgtgt ttggggtggt cgaacaactc
    18241 tttcgggctg ccctgctcta cgataacgcc gccgtccatc acgacaacgg tcgtggcaac
    18301 ttccagcgcg aacttgattt cgtgggtaac gacgaccatc gtccaacctt cccgcgccaa
    18361 ttccttcatg gcgttcaaca cgtcttgcac caattcaggg tcgagtgcgg aagtgggttc
    18421 gtcaaacaac atcagctcgg gctgaatcgc caatgcgcgg gcaatgccga cgcgctgctg
    18481 ctgaccgccg gaaagctggt agggatacaa atccactttg tcgcccaagc cgactttttc
    18541 cagcagtttc agagcctctt cgcgcgcttg ggcggcaggc ttgccctgta cggcaaccgg
    18601 tccttccatt acgttttcca aggcggtttt gtgcggaaag aggttgtatt gttgaaacac
    18661 catgcctgat ttgcggcgca gtgccaaaat atcgtgtttg cttggttttt tagaaaaatc
    18721 gattttcagc ggtcgctcgt tgtcgaactc gatttgtccg tcttcgggca tttccaacgc
    18781 gtttaggcat cgcagaaacg tcgttttgcc tgagccggaa ggcccgagga taacgaccac
    18841 ctgccctttg cccacatcca aattgatgcc gcgcaaaata gtgctttcgc cgaaggtctt
    18901 atggatattg cggattttaa tcataacaac tccttatttg gcgacataac ggtcgaaacg
    18961 tttttccaaa cgtgcctgaa tcaggaacag aaccttgcaa aaacaccagt aaaccaaagc
    19021 ggcttcgata tagacgggca aaaagtcata agtgcggttt gccgtttcct gcgcgacgcg
    19081 gaataattcc gttaccgtca cgactgccgc gagcgaggtg tttttaaaca aaccgataaa
    19141 ctcgttgctc aaaggcggca cggcaacgcg aaatgcctgc ggcgcgacga tgcggcggaa
    19201 cgtctgcata taggtcatac cgatggagaa acctgcttcc cattggcctt taggtacgga
    19261 caaaattgcc gcgcgtatgg tttcggaagc gtatgcaccg acattgagcg agaagccgat
    19321 aatggcggcg ggaatcggat cgatatagat accgacggaa ggcagcccgt aaaacacaat
    19381 cacaagctga accaacagcg gcgtaccgcg aatgacggaa atataaaatt ccaccaattt
    19441 cagcaggatt ttccgcacga tgccgccggc gggcataatc cgcaccaaag ccacggctac
    19501 cgcaatcatc ataccgataa cgaaagaagc tgccgccaaa ggcagagaga ccgcgaagcc
    19561 ggctttgacc ataggcaaaa acgcgctgac aatcatatcg gcgcgtgttt ccgtcataaa
    19621 cggcagcgaa gcaaggaaat tattgaacac tgatgtcttt tccgaagaat tgttcgccca
    19681 gttttttcag cgtaccgtcg gctttcagct cgttgattgc cgtactgaat ttcgccacgg
    19741 cttcgtcatt gcccttgttg acaatcaggc cggaaccgac tttttcatcg gcaggtgcgg
    19801 accaaacgat tttcacgccc gcattcgggt ttttcttcag atagtccaaa accgccaatt
    19861 cgtcgttcag ggttgcatcg gcacgttttt gttcaatcag ggtcagcgat tgcgccaaac
    19921 cgtccactgc caccaaatct gcgcctgcag ctttggcttt ttcgccgtag ttgctggtca
    19981 gggattgtgc ggttttcacg cctttgatgt cgtcgataga tttgatgttg ctgtcgttac
    20041 gggcaaccaa tacggcaccg ctccagctgt aaggatcgga tttgtcgaat gtcgcttggc
    20101 gttcggggct ggtcagaccg acttggtttg ccaccacgtc gaaacgcccc gccttcaaac
    20161 ccgccatcat cgaatcccat tgcgtttctt taaactcgac tttcacgccc agtttttccg
    20221 ccacggcgcg ggttacttcc acatcgtaac cggtcagttt gccgtccttg tcgtggtagg
    20281 taaacggtgc gtaagtgcct tccgtgccga cggtaaccgt gcctttattg ttgatgcgct
    20341 cgattaaaga accggaaact gccgattgtg caggcgcgga agatgctccg ctgccgcttt
    20401 ccgaaccgcc gcaggccgcc aaaaccaatg cggcaatgcc gccgagtacg aattttttca
    20461 acatcattct ctcctgtaaa aagtgtgcaa gtgcggcatt ctaagtgaaa ttgcggcaaa
    20521 cacaaagaat acttttttta tatggttatt ccaaatcgga atggaaaaga tatgccgaaa
    20581 ccgccggaag cggaaataaa gggggcggga aaggcgacgg gcggcacgaa accgaaaagc
    20641 ggcatcaggt atgcaaacgg cggattcacg cacgccgttc cggcaaaaat ccgcctgccg
    20701 attcggtgct ataatccatc cgaaacagat gacaccaaaa aggaacatct catgaacacg
    20761 ccccaatccg ccatcattcc cgaccacgcc caagccggca tctttatcga agccgacttc
    20821 gccgccaacc gcctcaacga tattaaagcc gcctgccgcg cttcgctcga cgcgttaact
    20881 gccttgaaag cccgctttcc ggacgatatt ttgggtctga ccatcgcctt cggcagcaaa
    20941 gcctgggcaa cattcggaca tacggacgaa ggcagtgaaa tcaaaccctt ctccgaaatg
    21001 ggcaacgggc ttgcgccgtc cacgcagcac gatatgtcca tccacatcca atccttccgc
    21061 caaaacgccg cctacgcgct tgcccaatcc gttttaggcg cgttcggcga cagcatatgc
    21121 gtcgcgtccg aagaacacgg tttgcgcctg tatcaggatc gcggactgga cggtttcgtc
    21181 gacggcacgg aaaacccgca gggcgatgaa aatgtccgcg aagtcgccat catccccgaa
    21241 ggaaaacccg atgcgggcgg cagctatgtc ctgctgcaaa aatacctgca cgatttgaaa
    21301 aaatgggatg ccgtccccgt cgccgaacag gaggcctcgg tcggacgcgg caaggaaacc
    21361 gacgacgaat tcggccgcga tgtccgcctg cccgattcgc acctcggccg cgtcaacctg
    21421 aaagaaaacg gagtcggcct gaaaatcgtc cgccgcagcc tgcccttcgg caaaatcagc
    21481 ggcgaacacg gtttgatgtt taccgcatac tgccgcacgc tgcacaatat cgaagcgcaa
    21541 ttattgagta tgttcggcga tacggacggc aaaaccgacc tgctgctccg acacctctcc
    21601 gccgccgttt cgggcggata ctactacgcg ccgtctgtcg aacgcctgca aaacctctaa
    21661 gccggacgat gccgtctgaa accatttttc agacggcatt ttcc
//
LOCUS       DAWEWP010000017        21064 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWEWP010000017 DAWEWP010000000
VERSION     DAWEWP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21064
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.017"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..176
                     /locus_tag="WGN53_000471"
     CDS             <1..176
                     /locus_tag="WGN53_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139500.1"
                     /translation="LVLIFVNPLYNVGIGSGALLGHWVTQYSGISCIGIAGMLVSAAG
                     LWVCLNLNRHIRT"
     gene            305..1741
                     /locus_tag="WGN53_000472"
     CDS             305..1741
                     /locus_tag="WGN53_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ0139501.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSAQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDRENTRRFWLGCGIAASLTVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFNGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLSFVVFNTASPD
                     DAGAVFNALFANANGWNAPQQANMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPISIILLLIIVLAPSGIPGFIYANF"
     gene            1752..2738
                     /locus_tag="WGN53_000473"
     CDS             1752..2738
                     /locus_tag="WGN53_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ0139502.1"
                     /translation="MKHFLSLFASILMSALIAAWFAQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYTLSDGIKAFLSGETPPTAQDGGSADMPPEAAASEAAPQTGEI
                     QEWKQDTDTAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYP
                     SFFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDR
                     ILEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTAHTLSGGKD
                     RYTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2735..3928
                     /locus_tag="WGN53_000474"
     CDS             2735..3928
                     /locus_tag="WGN53_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ0139503.1"
                     /translation="MNSKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDG
                     IASKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILAYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRIA
                     RQGQTMFWSWQNAMGGVCSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            4177..5730
                     /locus_tag="WGN53_000475"
     CDS             4177..5730
                     /locus_tag="WGN53_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ0139504.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(5820..7847)
                     /locus_tag="WGN53_000476"
     CDS             complement(5820..7847)
                     /locus_tag="WGN53_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ0139505.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQAQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLENLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVIFLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGNLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            8136..10142
                     /locus_tag="WGN53_000477"
     CDS             8136..10142
                     /locus_tag="WGN53_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ0139506.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGVIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            complement(10265..11356)
                     /gene="mltB"
                     /locus_tag="WGN53_000478"
     CDS             complement(10265..11356)
                     /gene="mltB"
                     /locus_tag="WGN53_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ0139507.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRVNEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAVIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(11505..12932)
                     /locus_tag="WGN53_000479"
     CDS             complement(11505..12932)
                     /locus_tag="WGN53_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0139508.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKRHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            13117..17244
                     /gene="mfd"
                     /locus_tag="WGN53_000480"
     CDS             13117..17244
                     /gene="mfd"
                     /locus_tag="WGN53_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0139509.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHALPDLAVNRQSDEPLQALKDFQTAFEGRILLCAESLGRRETMLGFLQQNGL
                     KAKSVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDSLKAAAVSTESSLPLGTSNLHGQIRQQPAPSP
                     VGEGWGEGKAVAAQTEFPASATNPLPSPLPQEREQSAAAVSDDLKTKSSLHPVANNLH
                     GQIRQQPTPSPVGEGWGEGKAVAAQTEFSAAATKPLPSPLPQEREQSAAVVSDSLKAA
                     AVSTESSLPPGKSNLHGQIQQQPAPSPVGEGWGEGKAVAAQSAIAVITESDLYQYVAR
                     SRVHNRRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLITMDLGGETNEMMLL
                     EYAGEAQLYVPVSQLHLISRYSGQAHENVALHKLGSGAWNKAKRKAAEKARDTAAELL
                     NLYAQRAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVC
                     GDVGFGKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSR
                     FNNSKATKAALEGLADGTVDIVIGTHKLVQDNIRFKSLGLVIIDEEHRFGVRQKEQLK
                     RLRANVDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREA
                     VLRELKRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQ
                     QRFNVLLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTP
                     EYITKDAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTE
                     MLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCET
                     VQQINAIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNN
                     NVDPTEIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            17393..17776
                     /locus_tag="WGN53_000481"
     CDS             17393..17776
                     /locus_tag="WGN53_000481"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ0139510.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVVCLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            17798..18640
                     /gene="kdsA"
                     /locus_tag="WGN53_000482"
     CDS             17798..18640
                     /gene="kdsA"
                     /locus_tag="WGN53_000482"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ0139511.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            18655..19095
                     /locus_tag="WGN53_000483"
     CDS             18655..19095
                     /locus_tag="WGN53_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ0139512.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     SVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKEAV"
     gene            19142..20428
                     /gene="eno"
                     /locus_tag="WGN53_000484"
     CDS             19142..20428
                     /gene="eno"
                     /locus_tag="WGN53_000484"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ0139513.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDASEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDAGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            20442..20720
                     /gene="ftsB"
                     /locus_tag="WGN53_000485"
     CDS             20442..20720
                     /gene="ftsB"
                     /locus_tag="WGN53_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ0139514.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(20761..21051)
                     /locus_tag="WGN53_000486"
     CDS             complement(20761..21051)
                     /locus_tag="WGN53_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ0139515.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYRKPPLAFLGRDEILPCCCYVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         5023 a         6398 c         5251 g         4392 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat acaacgtcgg tatcggcagc ggcgcactgt
       61 tgggacattg ggtaacgcaa tactcgggca tttcctgcat cggcattgcc ggtatgctgg
      121 tatcggcggc aggtttgtgg gtctgcctga atttgaaccg ccatattcga acctgaccgc
      181 gcgatgccgt ctgaagcccc tcccgccctt cagacggcat tttgattgaa gaaatatccg
      241 cccctgctta aaataacagg ctttgccgtg ttttaacgcc tattttttgt tccaaggatg
      301 gtttatgccg ttgctgtctg tcgagttcgc actgttcttt ctcgctttcc tgccgattta
      361 ctggggcttg gcgaaatacc cgtccgccca aaacctgctg cttctggctg ccggtatggg
      421 ctggctctac catatcggcc ccgtatttgc ggcaatcatc gtcctttatt cctcctgcgt
      481 gtaccttttg ggcgaactgc tccgttccga tcgcgaaaat acgcgccgct tttggctggg
      541 gtgcggcatt gccgcctcgc tgaccgtctt gggctttttc aaatatttcg actttttccg
      601 gccgctgatt gcccaatatg ccggaaaagg cggcgcaatc gacatcctga tgccgctggg
      661 gctttcgtat tacaccttcc agtcgctcgc ctatcttgtt tactgcttcc gcgccccgca
      721 cgccgcgcgt ttcagctggc acgagctgct gctgcacctg agttttttcc ccaccgttac
      781 ctccggcccg attatccgcg ccgccgcatt caaaagcgca gacggcgagc aggcaggcgc
      841 attggcgcaa atccgtaccc gccaaccgcg ctcacccgtc cgccccgcac tcgccgtttc
      901 cctgattctg ctgggtattg ccaaaaaatg gtggctggcg gggatgctcg cagaaaactg
      961 ggtgtcgccc gtgtttgaaa accccgccca attcaacggc tggggcgtgt tggcgggcgt
     1021 gtacggctat accttccaac tcttcctgga cttttccgga tattccgatt tggttatcgg
     1081 catggcgatg ctgctgggct tccgactgcc caaaaacttc tccgcaccgc ttcgtgccgc
     1141 gaatatccgt gcattttggg acaaatggca catcagcctt tccacctgga tacgcgacta
     1201 catctacatc cccttgggcg gcagcaaaaa aggctttttg cggacacagc tcaacctgat
     1261 ggcggcaatg gtactctcag gcatctggca cggctacggc tggaacttcc tcatttgggg
     1321 cgcgctgcac ggcacggcgc tggtgctgct caacacgggc gatcgctatt tcggacgcaa
     1381 cgcgctatgc cgtctgaaat accttgcgcc gctctcgtgg tttgttacct tccatttcgt
     1441 ctgccttagc tttgttgtct tcaataccgc aagccccgac gatgcaggcg cagttttcaa
     1501 tgccctcttt gccaatgcca acggctggaa tgcgccgcaa caggcaaaca tgctgctgct
     1561 tgcctcgttt gcatccgtga tgctgctcta cccttacctg caacgcgctt tcgacggcgc
     1621 ggtcaaaggt ttggaaaaaa tcccgatgtg gctgtggttt atcccgattt ccatcatcct
     1681 gctgctgatt atcgtccttg ccccctcggg gatacccggt tttatttatg ccaattttta
     1741 agggtttgga catgaaacac tttctttccc ttttcgcttc catattgatg tctgccctga
     1801 ttgccgcgtg gtttgcccaa aacccgatca atgcctactg gcagcagacc taccaccgca
     1861 acagcccgct cgaaccgctt gccgcctacg gatggtggcg gagcggtgcg gcgttgcaag
     1921 aaaacgccta caccctttca gacggcatca aagccttcct gtccggcgaa acgccgccga
     1981 cggctcaaga cggcggttcg gcagatatgc cgcctgaagc cgccgcatcc gaagccgccc
     2041 ctcaaaccgg cgaaatccaa gaatggaaac aggacaccga caccgccgcc gtccgcagcg
     2101 gcgacaaagt ctttttcgcc ggcgactcgc tgatgcaggg cgttgcaccc ttcgtgcaaa
     2161 aaagcctgaa acagcaatac ggcatcgaat ccgtcaacct cagcaaacaa agcacggggc
     2221 tgtcctaccc ctcattcttc gactggccga aaacgattga agaaaccctg aaaaaacatc
     2281 ccgaaatcag cgtgctcgcc gtcttcctcg gcccgaacga cccgtgggat ttccccgtcg
     2341 gcaaacgcta cctcaaattc gcttccgacg aatgggcgca agaatacctg aaacgcgtcg
     2401 accgcatcct tgaagccgca cacacgcacc gcgtccaagt cgtctggctc ggcatcccct
     2461 acatgaaaaa agccaagctc gacgggcaga tgcgctacct cgacaaactg ctttcggaac
     2521 atttgaaagg caaaatcatc ctgattccca ccgcgcacac cctgagcggc gggaaagacc
     2581 gctacacgga ctccgtcaac gtcaacggca aacccgtccg ctaccgcagc aaggacggca
     2641 tacactttac cgccgaagga caaaaactgc tggcggcaaa aataatggaa aaaatcgttt
     2701 ttgaaccaag tacgcaacca tcaagtacac agccatgaat tccaaacacc tcatcgcatt
     2761 ttccgccctg ttcgccgcca cgcaggcaga agccctgccc gtcgcctccg tcagcctcga
     2821 caccgttacc gtttccccgt ccgcccccta caccgataca aacgggctct tgaccgacta
     2881 cggcaatgcc gccgcctcgc cttggatgaa aaaactccaa tccgtcgcac aaggcagcgg
     2941 cgagaccttc cgtatcctgc aaatcggcga ctcgcatacc gccggcgact tctttaccga
     3001 cagcctgcgc aaacgcctgc aaaaaacttg gggcgacggc ggcataggct gggtttaccc
     3061 cgccaacgtc aaagggcagc gcatggcggc cgtccggcac aacggtaact ggcaaagcct
     3121 caccagcagg aacaacaccg gagacttccc gctcggcggc atcctcgccc acaccggcag
     3181 cggcggcagc atgaccctga ccgcatcgga cggcatagca agcaagcagc gcgtttccct
     3241 gtttgccaaa cccctgcttg ccgaacaaac cctgaccgtc aacggcaaca ccgtctccgc
     3301 caacggcggc ggctggcagg tactggatac gggcgcggca ctgcccctga ccatacacac
     3361 cgaaatgccg tgggacatcg gcttcatcaa catcgaaaat cccgccggcg gcattaccgt
     3421 ttccgcgatg ggcatcaacg gcgcacaatt aacccagtgg tcgaaatggc gtgccgaccg
     3481 tatgaacgac cttgcccaaa ccggcgccga tctagtcatc cttgcctacg gtaccaacga
     3541 agccttcggc gacaacatcg acattgccga cactgaacaa aaatggctgg ataccgtccg
     3601 ccaaatccac gacagcctgc ctgccgccgg catcctcatc atcggcgcgc ccgaatccct
     3661 gaaaaacacg ctcggcgtat gcggcacacg ccccgtccgc ctgaccgaag tccaacagat
     3721 gcagcggcgc atcgcccgtc aggggcagac gatgttctgg tcttggcaaa acgcgatggg
     3781 cggcgtttgc agcatgaaaa actggctcaa ccaaggatgg gccgccaaag acggtgtaca
     3841 cttctccgcc aaaggctacc ggcgcgcggc ggaaatgctc gccgacagcc tcgaagaact
     3901 cgtccgcgcc gccgcaatca ggcaataatc ggacaggagg cggacggtat ttccgcaaca
     3961 gggggatgcc gtctgaaacg cataccttca tattgcttca gacggcattc gcctcagaca
     4021 ttggaaagac gcaggcgaac ctccggcata cggcgcaaag gcggcgtttg atatgccgtc
     4081 tgaaggcaaa gatgataaac tgccgccttc cgttttcata cggcatattg ttttcaaatg
     4141 agggcgttcg ccgtccgcaa ccataaagga agtttcatga accggactta tgccaatttc
     4201 tacgaaatgc tcgccgccgc ctgccgcaaa aacggcaacg gcacggcagt gttcgacggc
     4261 aaggaaaaaa ccgcctaccg cgcgctcaag caggaggccg aagccgtcgc ggcgtatctg
     4321 caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg cggtttccaa ttccacagaa
     4381 tttattaccg cctatttcgc cgtctccgcc atcggcgcgg tcgccgtacc gatgaacaca
     4441 tttttgaaaa acagcgaata cgcgtatatc ctgaacgact gcaaggcgcg cttcctgttc
     4501 gcctcggcgg gcctgtcaaa agagttggcg ggcttgaagg cgcaaacgcc cgtcgaaaaa
     4561 atcatttgga cggacaaaag ccgtccggcc ggcgaaatgg cggaaggcga tgcctttttt
     4621 gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc gccaaccccg gataaatgat
     4681 ttggcacaca tcatctacac ctccggcacg acggggcatc ccaaaggcgc gctaatcagt
     4741 tacgccaacc tgttcgccaa cctgaacggc atcgaacgca tctttaaaat ctccaagcgc
     4801 gaccgcttta tcgttttcct gccgatgttc cacagcttca cgctgacggc tatggtgctg
     4861 ctgccgattt atatggcgtg ttcgattatt ttggtcaaat ccgttttccc gttttccaac
     4921 gttttgaaac aggccctgct caaacgcgcg accgtgtttt tgggcatacc cgcgatttac
     4981 accgcaatga gcaaggcgaa aatcccttgg tatttcagat ggttcaaccg cattcgcctg
     5041 tttatcagcg gcggtgcgcc tttggcggaa caaaccatcc tcgatttcaa agccaagttc
     5101 ccccgcgcca aattgctgga aggctacgga ctgagcgaag cctctcccgt cgtcgccgtc
     5161 aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc ccctgcccgg tttggaagcc
     5221 aaagccgtcg atgaagaatt ggtcgaagtg ccgcgcggcg aagtgggcga actgatcgtc
     5281 aggggcggtt cggtgatgcg gggctacctc aatatgcctg ccgccaccga tgaaaccatc
     5341 gtcaacggct ggttgaaaac gggcgatttc gttaccatag acgaagacgg ctttatcttt
     5401 atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc aaaacgtcta tccgcgcgaa
     5461 atcgaagaag aaatctacaa actcgatgcc gtcgaagccg ctgccgtcat cggcgtgaaa
     5521 gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat tgaaggaagg tatggatttg
     5581 ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg caaatttcaa aatccccaaa
     5641 caaatccact ttaaagacgg gctgccgcgc aacgctacgg gcaaggtatt gaaacgggtg
     5701 ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc gtccgaagcc ccgtccggca
     5761 aaaaaaatgc ggtgaatctg cttcaccgca ttttttggca aacgccggag cgaggatatt
     5821 tactctgcca attccacctg ctcgtgcgcc atcagttcct gaccgacatc atccaacaac
     5881 atcaaataac gttcgtagtt tttcaaaatg tcggcaatca gctcgtcctt gttcatatac
     5941 tgcacatcgt acccgacgcg cccgtcgaaa aaataagcgt agggtttgta agttgtctga
     6001 tgccggatat gcggcaggtt gccgtcgtta atcaactggt cggatacatc ctgcccgaca
     6061 gacttaatcc cgtacataaa atcgcgcatc gtctctttcc gaatgacgaa ctcgattgcg
     6121 ggctcgtcct gatgaaacat cttatcgacc cggacgctca agccgtattc ttccgaaagc
     6181 tcccgttgca actcgtgcat agcgggcgat gcggtatgtt tgaggaattt taaaatatcc
     6241 tgctcctgcg tctggctcat tatctgcacc agccgttctt tccacttgcc gcccgtccaa
     6301 aatacactgg tagggttaac ccgggtctca aaatatttct tatccgcact caatcctttc
     6361 cacaggctga aacacattat cagcatcagc agggcaaacg gcagggaaac aatcagggtc
     6421 atagactgca ggttgcctag cccgcccgag cgcatcagca aaacggcaac ggcagacatc
     6481 agcacgcccc acataaccgc ctgccaccgt ggtgcgctca agcccttgtc ccgagaggta
     6541 atattgttca ggacataaat cccggaatcg gcagaagtta caaaaaacag gaaaatgacc
     6601 agcaggctga cgatgctcgt caattcgggc agggggaggt aattaaagaa tttaaaaagc
     6661 agcgtttccg gagaggaggt catcttttcg agcattcccc ccgcaacccc gtcattcagc
     6721 caaatcgccg tattgccgaa gacggtaaac cacaaaacgc cgaacaggcc ggggatgagc
     6781 aaaaccccga agacaaactc gcggatggtg cgcccctttg aaatgcgcgc gataaacaaa
     6841 cccacaaacg gcgcccaaga acaccaccac gcccaataaa gcaccgtcca agattcaaac
     6901 cacggcttgt gttcccgttc gtacgcataa gttttaaaac tgaggcgcac cagattttcg
     6961 aggtagttcc ctatgttgtc gccgaatgcc gacaacaggt aaacagtggg gcccgccgcc
     7021 aaaacaaaaa acagcagcaa aaacgcaagg ctcaggttca actcgctcaa caccttcacg
     7081 cccttcccca cgccggatat tgccgaaacg acggcgaggg acatgacggc ggcgataatc
     7141 aaaacctgca cgctgaagct gttttcggca atccagccca tttcctgcaa tccggcgccc
     7201 agttgcgaag ccccgaaccc caatgtggtg atgatgccga aaaaagtagc aagcaacgcc
     7261 ataatatcaa tggcatcgcc gaaccttccg gaaatttttt ctttcaacag ggggtaaaaa
     7321 caagaacgca gggcaagcgg cagcttgtag cggaaaccga aataagccaa agccaatgca
     7381 atcgtaccgt acaccgacca agcgtgaacg ccccaatgga acaccgtgtg cagcaatgcc
     7441 tgctgctgcc tgtgttccgg cgtgccggcc gtaatgtccg aaaaataatg catcaacggc
     7501 tctgccacgc cgaaaaacat cagacccacg cccatcccgg ccgcaaacag catcgccagc
     7561 cacgacagga agccgaattc cggcacatct tcatcccgtc cgagcctgat gtttcccaaa
     7621 ctgctgaccg agagtatcag caggaaaccc agaaaaatgg aaaacgttaa aacataaaac
     7681 cagctgaact cggtaaaaat gacttctttt gcccgatcga gccacatctg cgcctgatcc
     7741 ggcacggtta aaaccaatac caccaaaaca cacacaaaaa acaaagtcgt caaaataacc
     7801 atcggattaa atgacgttcg gcgttctata aattcagaca gggacaaacc ttctcactcc
     7861 tttgttaaaa acagacaaac ccggtcatcg ggcaaagcgg tcaaaacctg ccggtaaaat
     7921 accggcttcc ggatgacgaa atgcacaaac cggccgaaat tgttataatc gaaaaaatta
     7981 aaaatcaata cggattattc tatcagattg atttatcgat atttattata tcattaatat
     8041 agtttgattt caaaccggcg gaaaatgcgc cgcccaccgc aggcgcgccc tccccgctat
     8101 cggggcgttt aacgccggat tgccgatgcg gtacaatggc tgatatgaag aaaattaccc
     8161 ctcaaaacct gcgccccctg ctttcggaaa gcttgggaca taccgatttt gtcaacgtcc
     8221 tcaacgcact gattaaattt ttgcgccgtg gcggcaaaaa atgtgcgggg gaacgtttcg
     8281 acctgattat cgacacattc aaacaagaca gggaattgct gtcccgcttc agccggtgtt
     8341 tttacatctg gctcgcgcaa atacacattt atccggcact catcaaactc ggcatcttct
     8401 cgcgccacag ctttgcccgg gaaatgggca tacgcatcta cgaacgcttc agcccgtcat
     8461 ataaagattt tgccaacttg ggcgaagtct tcctttatct tttccattcc gaaaacgacg
     8521 acaaatggct gcaaacgctc aatatccgcc aatggctggt tttatacgaa ctcatccgga
     8581 gccacgccga accgtccaaa ttgcagacgg cgggcatccg ccttgccgat gcgcgtttgc
     8641 gcgccatcga aatgctgtct gtctggacgg catccgaagc catcgaaccc gacctcatcc
     8701 gcatcgcccc gcgcctgctg gaagccgatt cttccttcgt cgccctccaa cgcgaaaccg
     8761 ccaaactggt cgaacactac cgcaacggca ccgcgcctta cgacaccgcc cacctcgaag
     8821 tgatgttcga ccaatgtttc agccagattg actatttgcg ccgcaaaggg acgggcgccg
     8881 gctccggttc gtcggtcaaa gtcgcccacc tgctcgaacg gctccggcag accgtagacc
     8941 gtctgaagct gctcaccgac atccaaaccg gcgccggcaa cagcaaccgc ctgaccatcg
     9001 cgctgatgaa ctccctcatc tacgcggcgg tcgaacaata cagcacccgc cacctgcgcc
     9061 gcagcagcat ccgtatgctc gcccgcagca ttaccgaaaa caaaagccac cacggcgaac
     9121 actacatcac ccgcaaccgc aaagaatatt tcaaaatgtt ctactcggcg gcaggcggcg
     9181 gcgtcatcat cgccctgatg gcgctgctca aaatccgcat cggcacactc ggcctcagcc
     9241 ccttcctcac ttccttgtcg gccgggttca actacggcat cggctttatg atcatccata
     9301 tgctgcactg caccgtcgcc accaagcagc ccgcgatgac tgccgccagc tttgccgaac
     9361 aggtcgatct caacgaaggc ggcaaagcgg tggacaacaa actcgccaag ctcctcatcg
     9421 acgtatgccg ctcccaaagc gtcgccgtct tcggcaacgt ttccatcgcc atccttttgg
     9481 cgtgcgccat atcgttcggc tatgcccatc tgtaccggca gcccatactc gatgcccaca
     9541 ccgccgccta ccagttcaaa tccatagaca tcatcgctta cccgacgctg tggtatgccg
     9601 ccattgcagg tctgtggctg ttctgctccg gcatcatcgc aggttttttc gacaaccgcg
     9661 ccgactacct caacctgcgc caacgcctgc ctttcaaccc cttgctgcgt aaaatcatgc
     9721 gccccgggcc ccgccgcgtc ctcgccgcct acatccacaa acactacggc tcgttggtag
     9781 gcaacttcat cttcgggatg ctcttgggta tgaccggcta tttcggacac ctcctcgggc
     9841 tgccgctgga catccgccac gtcgcctttt cctccgccaa cctcggctat gccgccgtca
     9901 gcggcaacgt cggtttgggc acgttcgtac tcggcatttt cagcgtcctc gccatcggcc
     9961 tggtcaacct ctgcgtcagt ttcagcctcg ccctcttcgt cgccctgcgc tcgcgcggca
    10021 cgaaaatcgg cagcatccgc aatctgatta aaagtttttg gaatcagatt aaaagcaatc
    10081 cctgcatact tttcctcccg cccgccaaag aacagggaca tcctccttcg gacaagcctt
    10141 gaccggcaat gccgtctgaa gcgggattcg ccccgaatac cgccctgatg cgggaaatcc
    10201 ccataaaagg atgcaaaaat gccgtctgaa tcgaaacgtg gttcagacgg cattttaaaa
    10261 aacattacaa tcccgggccg ccaagcgaat tggcaatgtc cctgaccgcc gttacataca
    10321 tccgactgtg attgtactgc cataccgtat aaaaattgtt caagcccaaa taatattcaa
    10381 acacgccggg tgcggtttcc agtttgaaca aaaccgcctt ttcatcatcg gcaagctctt
    10441 cgccggggat gatgccgtac gccttcaaat ccgccaccgt ccgcgtcagg gcggtttttt
    10501 cgccaatgat tgcctgaaca tccgcacccg gcgccaatgt tgcagacacc agtattttcc
    10561 cgcccgtgcg ccaaccgtgc tgcttcatat aattggcaac cgatgccgcg acatcgccga
    10621 tgttgcccca tatgtcccga tgtccgtccc cgtcataatc caccgcccat ttccggtagc
    10681 tcgaaggcat aaattgcggc atccccattg cgcccgcata gctgccttta aaggcgaaaa
    10741 catcgccgcc ttcttctttt gccagcttta aaagctcgac caattctttt tggaaaaacc
    10801 cggcgcggcg ggggtaatca aagcctaagg tcgccaatgc gtccgccaca cggaaactgc
    10861 ccgtattttt gccgtaattc gtttcaatcc cgataaccgc cacgataagt tcggcaggca
    10921 cgccgtattt ttgcgccaca tcatcgataa gcgcgcggtt ttccgcataa aaccggcgcg
    10981 cgccgcgaaa tttcgccttg cccgaatttc ccgtgcggaa cacataccac ggacgcgatg
    11041 tggagggtcg gtgcataatc ttgacgatgt ccgccttgta agccgctttg tcaaaaaaat
    11101 cctgccattc cgcccgggaa aaatcccctt tcccgacttc atcgtccaca aaacggcgga
    11161 catttgcatt ggcggcaaac ccgctgtcgg ataccggtac ggctgccgcg tcaaacgcgg
    11221 ggcggctttc ttttttcatt tcatccgcgc ggggggcttg ggcttcattt acccggggcg
    11281 ggcgtgcctc catcgccgta caggcagaca aagccgccaa acaaattgcc agcggcagta
    11341 tttttctctt tttcataaat atgttccgaa caaatagggt aagtgggaaa gcggcgcaag
    11401 gggcgcggtg cgaaatgcgg catccgccgc aatcggcggc tttgtggaat accgcgcgtt
    11461 gccttttgca ccgcctgaaa tccgtatgcc gccgtatttg cccgctatcc gcaatatcgg
    11521 cagtccaata tatctttgcg gatgtcgttc agcaggaagg ctgcggtgtc ttcgtgttcc
    11581 gcctgtacga agacaaagag tcgggcgatg attttgccga tgaagacttt ttgcagggcg
    11641 caggcgtcgg tcagggtgtg ttcctgcagg aagctgcgga tgtcttcggg caaagtgtag
    11701 tcgcgggcga cggcggcaaa gcgggcatcg gtttgcaggc ggtcgagcag ggtttggttt
    11761 ttgtccaact tcatgcctgc ttctttttct tcggcggtgg cgcggacgaa ctggtcgtaa
    11821 atttcggcat aaagcatatc gttcgggcct tcaaaaatcg tgaagggacg gatgtcgata
    11881 gcgatattgc cggcggtgtg tccgcgttca aaacccttcg cgcccaaaag tttttgcaac
    11941 atttgcgcgg cggcgtaagt gtattccgtg gcgagggttt tgacgatgtt cgcctccatc
    12001 agctgatggg cgacgggtgc aacgggcgaa acggaatggc agacgtagcg gtaaagaatc
    12061 tcggaaacct gatggcggcg ccggatttcg cggcgttcgt aatcgacgaa tctgatgtcg
    12121 ttgcggacgt atcgttccag gttttcaagg atgtattcca taatgccgtg cgtcatgccg
    12181 atcagttgca ggcggctgcg gataaagatg ttttggaacg cgcgcaaacc tgcggcgtcg
    12241 ctctgggaga gtttcatcac ggcggttgca ggcatttcgg catcgatgcg gttgacggcg
    12301 taacggacgg cgcgcaagcc ttcggatgcg agggtttcgc agcggatgta tgttttgggg
    12361 acgagcagca ggtcgatgac tttggcgagt ttgccgtttt tgcgttcttt ggcggcaacg
    12421 aggaggaagt cgctttgcga gttgccctgc cagtattttg cggcgttgac gtaaatggtt
    12481 tgtccgtcgg tatattcgta gtaagactgc atttctcggg caatcgccgc gccggaggtt
    12541 tcgggttcgg taacgcccaa accgccgccc tcgcctttga aaaccatgtc caaaccctgt
    12601 gcgatttgcg cttcatcgcc gaactcttgc agtggctgca acaccagcgc gccttcgatg
    12661 ccggtacgca gcgtaacggg tacgccgtaa tgccccgcaa tccgcaatac ttcctgaatt
    12721 tcaaactggc tgcccttgcg gccgccgtgt cttttgtcga ggaagggcaa cagcaaaccc
    12781 gcctgcttca aggcaagcca tttgtcttcg ggcaggtatc gcatcaggtc gataccgtct
    12841 gaaaaaatgc ggcggaatgc ggattcgatg tgctttaaaa aagcagccgt gtccatagtt
    12901 gacggctgcg cgctcggttc ggtgtgtatc atcggcttcc tctgtcggtt cccattaatc
    12961 ggcggccggt caaaccgcct gccacagttt agagttgatt ttctaaactt taccacaaag
    13021 tgcgccgggc aacaatccgc cggcttttca gacggcatcg cgcaccctcc cgtgctaaaa
    13081 tgaccgtttg catcactgtc cgccgattgc cgcactatga cctaccccat ccccaaaccc
    13141 cgtgaaaaat cccgttggcc caatctttcg caaggctcgc tgcccttggc tttggcgcgt
    13201 tatctgccgc acaagcggct caaggtcgtg ctgacccaag atgcggaaca ggcgttgcgc
    13261 cttcagacgg catggcggtt tttccgtccg cacgacacgg cggtgttctt gccggactgg
    13321 gaaacgctgc cttacgagcg tttttctccg catcaggatt tggtgtcgga gcggctctcg
    13381 gcgttgtggc agattaagag cggcgcggcg gacgtgttgt ttgtgccggt tgccacggcg
    13441 atgcagaagc tgccgcccgt gccgtttttg gcggggcgca cgttttggct gaaaacagga
    13501 cagactttgg atataggccg tctgaaaacc gatttggtag atgcgggcta caaccatgtt
    13561 tcccatgttg ttgcggcggg cgagtttgcc gtgcgcggcg gcattgtcga tttgttcccc
    13621 atgggcagcg aaatgcccta ccgcatcgat ttgtttgacg atgaaatcga cagcatcaaa
    13681 acctttgata cggacacgca gcgcaccatc tcccccgttt ccgaaatccg cctgctgccg
    13741 gcgcacgaat tccccaccga cagcgaggcg caaaaaatct tccgcagccg cttccgcgag
    13801 gaagtcgatg gtaatccgaa cgatgcggct gtatacaaag cagtaagcaa cggccacttc
    13861 ggtgcgggcg tggaatatta cctgccgctg ttttttgaaa acgagctgga aacgctgttt
    13921 gactatatcg gcgaagacgc gctgtttgtc tctttgggcg atgttcacgc cgaggcaaac
    13981 cgcttttgga gcgacgtcaa atcgcgttac gccatggcgc agggcgacga aacctatccg
    14041 cctttgcttc cacagcattt gtatctctct gccgatgttt ttgcaggccg tctgaaaaac
    14101 tacggacagg tgctgcccga tgtttccggc aaggaacacg ccctccccga ccttgccgtc
    14161 aaccgacaat ccgacgagcc tttgcaggca ttgaaggatt ttcagacggc gtttgaagga
    14221 cggattttgc tgtgcgccga aagtttggga cggcgcgaaa ccatgctcgg cttcttgcag
    14281 caaaacggtt taaaagccaa aagcgtgtct gattggcaag gcttcttgtc ggcacatgag
    14341 ccgctgatga ttacagtggc accgttggct tacggattca aactgggcgg attgcaatcc
    14401 ccgaaccagc agcaaactac ttccgcctcc gaaggagaag gcgatgcggt taccgaccaa
    14461 accgaatttt ccgcatccgc aacaaaccct ctccctagcc ctctcccaca ggagagggaa
    14521 caaagtgcag ccgccgtttc agacagtctg aaagcagccg ccgtttcaac cgaaagcagc
    14581 ctgcccctcg gtacaagtaa tctgcacggg caaatccgac agcaacctgc cccttccccc
    14641 gtgggggagg gttggggaga gggcaaagca gttgccgctc aaaccgaatt tcccgcatcc
    14701 gcaacaaacc ctctccctag ccctctccca caggagaggg aacaaagtgc agccgccgtt
    14761 tcagacgacc tgaaaaccaa aagcagcctg catcccgtcg caaataatct gcacgggcaa
    14821 atccgacagc aacctactcc ctcccccgtg ggggagggtt ggggagaggg caaagcagtt
    14881 gccgctcaaa ccgaattttc cgcagccgca acaaaacctc tccctagccc tctcccacag
    14941 gagagggaac aaagtgcagc cgtcgtttca gacagtctga aagcagccgc cgtttcaacc
    15001 gaaagcagcc tgccccccgg taaaagtaat ctgcacgggc aaatccaaca gcaacctgcc
    15061 ccctcccccg tgggggaggg ttggggagag ggcaaagcag ttgccgctca aagtgccatc
    15121 gccgtcatca ccgaatccga tctctaccaa tacgtcgccc gttcgcgcgt ccacaaccgc
    15181 cgcaagaaac acgccgccgt ttcagacggt ctgttgcgcg accttgccga aatcaacatc
    15241 ggcgaccccg tcgttcacga agaacacggc atcgggcggt atatgggctt gatcacaatg
    15301 gatttgggcg gcgaaaccaa cgaaatgatg ttgcttgaat atgcaggcga agcgcagctt
    15361 tatgtgcctg tttcgcaact gcatttaatc agccgctact ccggtcaggc gcatgaaaac
    15421 gtcgccctgc acaagctcgg cagcggcgcg tggaacaaag cgaaacgcaa agccgccgaa
    15481 aaagcgcgcg ataccgccgc cgagttgctc aacctctacg cccaacgcgc tgcccaatcg
    15541 ggacacaagt ttgaaatcaa cgagttggac tatcaggcgt ttgccgacgg tttcggctac
    15601 gaggaaaccg aagaccaggc cgccgccatc gccgccgtga ttaaagacct gacacaggcg
    15661 aagccgatgg accgactcgt gtgcggcgat gtcggctttg gcaaaaccga agtcgccctg
    15721 cgcgccgcgt ttgtggcggt gatgggcggc aaacaggtcg ccgtactcgc cccaaccacg
    15781 cttctagtcg agcagcacgc gcaaaacttt gccgaccgtt tcgccgattt ccccgtgaaa
    15841 gtcgccagcc tttcgcgttt caacaacagc aaagccacca aagccgcact ggaaggcttg
    15901 gcggacggca cggtcgacat cgttatcggc acgcacaaat tggtgcagga caacatcaga
    15961 ttcaaaagct taggtttagt gattatcgat gaagaacacc gcttcggcgt gcgccagaaa
    16021 gaacagctca aacgcctgcg cgccaatgtc gatatcctta ccatgaccgc cacgccgatt
    16081 ccgcgcaccc tcagcatggc actcgaaggc ctgcgcgact tctcgctgat taccaccgcg
    16141 cccagccgcc gcctcgccgt gaaaaccttt gtcaaaccgt tcagcgaagg cagcgtgcgc
    16201 gaagccgtgt tgcgcgaact caaacgcgga ggacaggtat ttttcctgca caatgaagta
    16261 gatacgattg aaaatatgcg cgagcggctg gaaaccctgc tgcccgaagc ccgcatcggc
    16321 gtggcgcacg gacaactgcg cgagcgcgag ctggaacaag tcatgcgcga ctttttacag
    16381 caacgattca acgtgttgct ctgttccacc atcatcgaaa ccggtatcga cattcccaac
    16441 gccaacacca tcatcatcaa ccgcgccgac aaattcggac tggcgcaact gcaccagctt
    16501 cgcgggcgcg tcggacgcag ccaccaccaa gcctacgcct acctgctcac gcccgaatac
    16561 atcaccaaag acgcagaaaa acgcctcgac gccatcgcgg cagcagacga actcggcgca
    16621 ggtttcaccc tcgccatgca ggatttggaa atccgcggcg caggcgaaat cctcggcgaa
    16681 gggcagtccg gcgaaatgat acaggtcggc ttcacgctct acaccgaaat gctcaaacaa
    16741 gccgtgcgcg atctcaaaaa aggccgccaa cccgacctcg acgcgccgct gggcatcacc
    16801 accgaaatca aactgcacag ccccgcccta ctgcccgaaa gctactgccc cgacatccac
    16861 gagcgtctcg tcctctacaa acgcctcgcc gtctgcgaaa ccgtgcagca aatcaacgcc
    16921 atacacgaag aactcgtcga ccgcttcggc ctgcccgaac aacccgtcaa aacccttatc
    16981 gaaagccacc acctgcggct tatggcaaaa gaattgggta tcgatgccat tgatgcggcc
    17041 ggcgaagcgg taacggtaac cttcggtaaa aacaataatg tcgatccaac cgaaatcatc
    17101 ctgctgattc agaacgacaa aaaataccgc cttgccggcg ccgataagct gcggtttacc
    17161 gcagagatgg aaaatatcga ggtcagaatc aacaccgtaa aaaacgtttt aaaaaccttg
    17221 caaaacagat gcctgcccaa ataaagccga caccgcaatg ccgtctgaaa caccgttttc
    17281 cttgtccgaa agccgccatt atgaatttga aggaaactcc actataatac ggcattcaga
    17341 tctccagacg gcatcgcgcc cgtcagaccg cacacaaacc aaaaggaaat acatgttccg
    17401 tactatgctt ggcggaaaaa tccaccgcgc caccgttacc gaagccgatt tgaactacgt
    17461 cggcagtatt accgtcgatc aagacctgtt agacgcagca ggcatctacc ccaacgaaaa
    17521 agtcgccatc gtcaacaaca acaacggcga acgttttgaa acctatacca tttcaggcaa
    17581 acgcggcagc ggcgtggtct gcctgaacgg ggcggcggcc aggctggtac agaaaggcga
    17641 tatcgtcatc atcatgtctt acgtccaact ctccgaaccc gaaatcgccg cacacgaacc
    17701 caaagtcgtc ttggtagacg gaaacaacaa aatccgcgac atcatctcct acgagccgcc
    17761 gcacaccgtg ctgtaaccct gaaaacggac attgaccatg gatattaaaa tcaacggcat
    17821 taccgtcggt aacgatgctc ctttcgtact gttcggcggc atcaacgttt tagaagattt
    17881 ggattccacc ctccaaacct gtgcgcatta cgtcgaagtt acccgaaaac tcggtattcc
    17941 ctatatcttt aaagcctctt tcgacaaggc aaaccgttcc tccatccatt cttatcgcgg
    18001 cgtaggcttg gaagaaggct taaagatttt tgaaaaagtc aaagcagagt tcggcatccc
    18061 cgtcattacc gacgtacacg aaccccatca gtgccaaccc gtcgccgaag tgtgcgatgt
    18121 catccagctt cccgcctttc ttgcgcggca gaccgatttg gtggccgcaa tggcggaaac
    18181 gggcaatgtc atcaacatca aaaaacccca gttcctcagc ccttcgcaaa tgaaaaacat
    18241 cgtggaaaaa ttccgcgaag ccggcaacgg gaaactgatt ttatgcgaac gcggcagcag
    18301 cttcggctac gacaacctcg ttgtcgatat gctcggtttc ggcgtgatga aacaaacctg
    18361 cggcaacctg ccggttattt tcgacgttac ccattccctg caaacccgcg atgccggttc
    18421 tgccgcatcc ggcggtcgtc gcgcacaggc tttggatttg gcacttgcag gcatggcaac
    18481 ccgccttgcc ggcctgttcc tcgaatcgca ccccgatccg aaactggcaa aatgcgacgg
    18541 ccccagcgcg ctgccgctgc accttttaga agatttttta atccgcatca aagcattgga
    18601 cgatttaatc aaatcacaac cgattttaac aatcgagtaa cacggtttcg ccttatgatg
    18661 cagactttcc gaaaaatcag cctgtatgcc gcaaccttgt ggctcggtat gcagattatg
    18721 gcgggttata tcgtcgcgcc ggtgctgttc aaaatgctgc ccaaaatgca ggcgggcgaa
    18781 attgccagcg tattgttcga catcctctct tggagcgggc ttgccgtttg gggcgcggta
    18841 ctggctgccg cctttgccgc cctaacccgg cggcaaaccg ccctgctgct ttttttattg
    18901 tccgcccttg ccgccaaccg attcttgatt acacccgtta tcgaggcgct gaaatacggg
    18961 catgagaatt ggctgttgtc gtttgtaggc ggatccttcg gaatgtggca cggcatttcc
    19021 agtattgttt ttatggcaac cgccctactt tcagcagttt taagttggcg gctttccggc
    19081 aaagaggccg tctgaagccc tcccattttt tacctccctt cacttcactt ggagaacatt
    19141 catgagcgca atcgttgata ttttcgcccg cgaaattttg gactcacgcg gcaaccccac
    19201 agtcgagtgt gatgtattgc tcgaatctgg cgtaatggga cgtgcagccg taccgagcgg
    19261 tgcgtccacc ggtcaaaaag aggctttgga actgcgcgac ggcgacaaat cccgttattc
    19321 gggcaagggc gtattgaagg cggtcgaaca cgtcaacaac caaatcgccc aagccctcat
    19381 tggtatcgat gccagcgaac aatcttatat cgaccaaatc atgatcgaat tggacggtac
    19441 tgaaaacaaa ggtaacttgg gtgccaacgc gactttggcc gtttctatgg ccgtagcacg
    19501 cgcagccgct gaagatgcag ggctgccgct ttaccgctac ttgggcggcg caggtccgat
    19561 gtccctgccc gtaccgatga tgaacgtcat caacggcggc gaacacgcca acaacagcct
    19621 gaacatccaa gagtttatga ttatgcccgt cggcgcaaaa tctttccgcg aagcgttgcg
    19681 ctgcggtgcg gaaattttcc acgccttgaa aaaactgtgc gacagcaaag gcttcccgac
    19741 cacagtcggc gacgaaggcg gtttcgcccc caacctgaac agccacaaag aagccctgca
    19801 actgatggtc gaggcgaccg aagccgccgg ctacaaagcg ggcgaagacg tattattcgc
    19861 attggactgc gcctccagcg agttctacaa agacggcaaa taccacttgg aagccgaagg
    19921 ccgctcctac accaacgcgg aatttgccga atatctggaa agtctggtca acgagttccc
    19981 catcatctcc atcgaagacg gcatggatga aaacgactgg gaaggctgga aactgctgac
    20041 cgaaaaactg ggcggcaaag tccaactcgt tggcgacgac ctcttcgtta ccaacccgaa
    20101 aatccttgcc gaaggcattg aaaaaggcgt ggcaaacgca ctattggtca aagtcaacca
    20161 aatcggtact ttgagtgaaa ccctgaaagc cgtcgactta gccaaacgca accgctacgc
    20221 cagcgtaatg agccaccgct ccggcgaaac cgaagacagc accattgccg acttggcagt
    20281 cgccaccaac tgtatgcaga tcaaaaccgg ttctttgagc cgttccgacc gcatggcgaa
    20341 atacaaccaa ctgctgcgta tcgaggaaga attggcggaa gccgccgact accccggcaa
    20401 agccgcattc taccaactgg gcaaataaaa aaggttaagg tatgaagtgg gtaactgtcg
    20461 ttttatcctt cgcacttgtc tgttgccaat acagcctctg gttcggcaaa ggcagcatcg
    20521 gacgcaacag cagtctgaga gaacagattg ccgttcaaga agaaaaaaac cagacactcg
    20581 ccctacgcaa tcattccctt gccgccgaag tctatgattt ggaaaacggt caagaagcca
    20641 tttcggaaat cgcccgggta gaactgggtt atatccaaga cggtgaaacc ttttaccgac
    20701 tcatcaggca taaccggtaa taccgtcaaa aagccgtccg aaccaatgtt cggacggctt
    20761 ttatttcaac aaactgtcag acagcccctc atcctccccc gacaaaccgc aatccagcct
    20821 gacatccccc tcgacgtaac agcagcacgg cagtatctcg tcccgcccca aaaaagccaa
    20881 aggcggcttc cgataagtaa cgcttccctc caaaatcttc acccggcacg atccgcaata
    20941 tccgcttcgg cactgatatt ccaccatatg ccccgtccgt tccaagcctt ccaacagggt
    21001 ctcgccctcc aagagctcaa aaaaaccctt attcgtacca atgcgcgcca tttccgacca
    21061 atca
//
LOCUS       DAWEWP010000018        20934 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWEWP010000018 DAWEWP010000000
VERSION     DAWEWP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20934
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.018"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(79..771)
                     /locus_tag="WGN53_000487"
     CDS             complement(79..771)
                     /locus_tag="WGN53_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0139516.1"
                     /translation="MFHAFILRIYTMIKNISKLINHAVLIGFIPNIVFAYPIFAENPA
                     SYQNIHVRSWKARRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKTQM
                     RASFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGETV
                     LLADPSLGHVSMSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQTR
                     FTVEQIQMRQKR"
     gene            complement(1077..1634)
                     /locus_tag="WGN53_000488"
     CDS             complement(1077..1634)
                     /locus_tag="WGN53_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139517.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLEIWHGDRVFMH"
     gene            complement(1879..2037)
                     /locus_tag="WGN53_000489"
     CDS             complement(1879..2037)
                     /locus_tag="WGN53_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139518.1"
                     /translation="MKPPLPICTLSALAACTLSGQGGTQVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2034..2876)
                     /locus_tag="WGN53_000490"
     CDS             complement(2034..2876)
                     /locus_tag="WGN53_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0139519.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3115..4035
                     /gene="pyrB"
                     /locus_tag="WGN53_000491"
     CDS             3115..4035
                     /gene="pyrB"
                     /locus_tag="WGN53_000491"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ0139520.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4045..4503
                     /locus_tag="WGN53_000492"
     CDS             4045..4503
                     /locus_tag="WGN53_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0139521.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4568..5233
                     /locus_tag="WGN53_000493"
     CDS             4568..5233
                     /locus_tag="WGN53_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139522.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALDLGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5296..6513)
                     /locus_tag="WGN53_000494"
     CDS             complement(5296..6513)
                     /locus_tag="WGN53_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0139523.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFDRSE"
     gene            6655..7200
                     /gene="def"
                     /locus_tag="WGN53_000495"
     CDS             6655..7200
                     /gene="def"
                     /locus_tag="WGN53_000495"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ0139524.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7287..8213
                     /locus_tag="WGN53_000496"
     CDS             7287..8213
                     /locus_tag="WGN53_000496"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ0139525.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8292..9551
                     /gene="rsmB"
                     /locus_tag="WGN53_000497"
     CDS             8292..9551
                     /gene="rsmB"
                     /locus_tag="WGN53_000497"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ0139526.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAILRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIPARALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9517..10116
                     /locus_tag="WGN53_000498"
     CDS             9517..10116
                     /locus_tag="WGN53_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ0139527.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10116..12233
                     /locus_tag="WGN53_000499"
     CDS             10116..12233
                     /locus_tag="WGN53_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ0139528.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLISTASLSGNMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRQREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVKTYA"
     gene            12226..13503
                     /locus_tag="WGN53_000500"
     CDS             12226..13503
                     /locus_tag="WGN53_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ0139529.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QVRPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKHGAAKIETGPVFDKLGNSAAIQEMNCEVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGIAFIIGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13594..14787
                     /gene="dprA"
                     /locus_tag="WGN53_000501"
     CDS             13594..14787
                     /gene="dprA"
                     /locus_tag="WGN53_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ0139530.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRASARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAERGLIVSEFPLDTRPFAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPRLLQNEGASSYSINKGMPEKRITAVQTASDQLSLPEGKMPS
                     EKTENRPVGGSILDRMGFDPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRY
                     QRIRT"
     gene            14813..15274
                     /locus_tag="WGN53_000502"
     CDS             14813..15274
                     /locus_tag="WGN53_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ0139531.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15346..17652
                     /gene="topA"
                     /locus_tag="WGN53_000503"
     CDS             15346..17652
                     /gene="topA"
                     /locus_tag="WGN53_000503"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ0139532.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17725..18420)
                     /locus_tag="WGN53_000504"
     CDS             complement(17725..18420)
                     /locus_tag="WGN53_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139533.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            19111..19488
                     /locus_tag="WGN53_000505"
     CDS             19111..19488
                     /locus_tag="WGN53_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0139534.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNV
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19577..20146
                     /gene="rsmD"
                     /locus_tag="WGN53_000506"
     CDS             19577..20146
                     /gene="rsmD"
                     /locus_tag="WGN53_000506"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ0139535.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20269..20520
                     /locus_tag="WGN53_000507"
     CDS             20269..20520
                     /locus_tag="WGN53_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ0139536.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            20617..20700
                     /locus_tag="WGN53_000508"
     tRNA            20617..20700
                     /locus_tag="WGN53_000508"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20651..20653,aa:Tyr,seq:gta)
     gene            20731..20804
                     /locus_tag="WGN53_000509"
     tRNA            20731..20804
                     /locus_tag="WGN53_000509"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20763..20765,aa:Gly,seq:tcc)
     gene            20814..20888
                     /locus_tag="WGN53_000510"
     tRNA            20814..20888
                     /locus_tag="WGN53_000510"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20846..20848,aa:Thr,seq:ggt)
BASE COUNT         5261 a         5299 c         5683 g         4691 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat ataaaataaa
       61 tgggcaaaaa tcggtttatt atcgtttttg ccgcatttgg atttgttcta ccgtaaaacg
      121 tgtttgacga acgggattct tattaaaaaa catctgattt ctaacaaaat cagtattttt
      181 tggcacgatg gctaaaattt ttccttccat ttcgccatca cgtgttttcc atgcgctcaa
      241 gaattgtgat ttgctcattg agacgtgccc cagcgatgga tcagccagca aaacagtttc
      301 tccgttaata ccgttcaata ccgaaaaatg gttgttttta cggtatttta aatacacaat
      361 tacaggaatt tttagttgta ccaactgttc aaatggcaaa gcataacctt gtgcttcaaa
      421 acccagttcg ggcattatgc gttgcatatc atcaaaagaa gcacgcattt gggttttatc
      481 cattttgtcc aagatttccg cttcagaata atgtctgcca taaaaattat tcagtaacgt
      541 ggcaatcgaa gccgcgccgc aagaaaaatc caaatcttgt tttactatgc cggaatctcg
      601 ccgtgctttc caactccgta catggatgtt ttggtaagaa gcggggtttt cagcaaatat
      661 agggtaagca aaaacaatat ttgggataaa gccaattaaa accgcatggt ttattaattt
      721 agaaatattt tttatcatag tatatatgcg tagaataaac gcatgaaaca taaaaaaata
      781 aaatcatatg caattttatt gcatgaataa atatgaataa aatagataat gatgggagta
      841 aatacgccat gtattttgga agtttaaatt tattaataat aaaataattt atcgtagcgc
      901 aaataaatcc caaaattgga acgattagaa aaaaaattaa tacacccatc atcatgcaaa
      961 ctctatatta ataaatagct aactaattct attgtggaaa ttagttagct aactaaaagt
     1021 tattaatgat tatttccgag aattgactgc attgttggca gcattggccc caaaacctag
     1081 tgcatgaata cccggtctcc atgccaaatt tccagccaat ccgcctccgg cagcagcagc
     1141 cagccctgtt ttgccgctac tcctgttgcc gcaccgattc ctgccgcagt agccgcgcct
     1201 tgcgcagttc ctaatttacc atgattatac aaattagcac catgatactc ccatgcacct
     1261 aatgcaccgc caaaagcagc ggctgcaata atgggaacaa attcaccttg tgtttctttc
     1321 atttcagcct gtgataattg aattgctttc acattttggc tgtcaaaaac ttggctgtct
     1381 aaattttgcg ccattacagg tgtaatcatc atagccatta cagttgcaat tttcgttgcg
     1441 ctggtttgca cataaatagg attagcaaat tcgctttgat tgcgttcagt gttgatgtag
     1501 ctaatactgc tttctagttt gaatttaccc ttgtcagtca ataattcttc caaacttaaa
     1561 ggtaagtcag cataagcaat ttggctggca acagtcaaaa taaaatctat tagacatttg
     1621 tgtttttgca tcatttcgtt tgattttcta gtttttgaga atgatacaaa gtttttttac
     1681 aaagtaaaga gtcactctga aaaacatttt ttcattataa atcaaaatat tgatagaata
     1741 aatagcgagc atcgattcac ggtgtacttt agtgcaaagc gtacaacgcg agcctgaacc
     1801 accagccgca acaggaaaag aaagccgata gagtgcaatg cttgccaacg tgcaagcgag
     1861 cttgcaagaa cgcttggctc aacgagagca ggcaagacag aaagcagaaa gcaggatagg
     1921 agcggtaacg caaaggtctc gggctttgat ttcgccgtaa acctgagtgc cgccttgtcc
     1981 ggaaagggtg caggcggcga gtgccgacag ggtgcagatg gggagggggg gtttcatttg
     2041 gggtcgcaac ggaagtggta tgcgcagatt tcaaaaccgt ttttgaaata caggcggtgc
     2101 gcgtcggcac ggtcgtggtt gacgtggacg ttgaggtgga ttttggttac ccctgtttcc
     2161 gcgccgattt tgcggacttc ttccaaaagg cgcgaggcgt agcctttgcg gcggctttgc
     2221 ggcagggtaa cgatgtcatc gatgtggatg tggcggccgc tggcgagggt gcaggcttcg
     2281 cggaagccgc agacggcgac ggcattgtgt ttgccttctt caaaaatacc cagcaggcgg
     2341 tagccttggg ggcgttggac tttgttgatc tgttcggtaa agcggttgat gtcggtcagg
     2401 gcggaacgca aaacgctcaa ggctgcaaag gcggtggcgg tgtcgtccgc gccgatttcg
     2461 cgcaaaacgt aggatgcgcc cgaggcggtc tgttcctgtg ctttctcggc ggcgtgtttt
     2521 tcttcgattg cctgtgccag catgacgtgt tcgtcggcag ggttgttttg tccgccctgt
     2581 tcgcgttctt cgagcagggc tttgcagtcg atgacgcgca ggtcgttgtc ggcggcaaag
     2641 tccatcagga agcggaacat ttgggggttg tcggtttcca gttttttatc gacggcgacg
     2701 cagcggatgt tgtccaccag catggggcgc acccatttcg aataggaaag cctgtggtct
     2761 ttggtgaacg aggacagaat gccgcacagg cgttccgccc agtcgctggg acggaaaatc
     2821 ttgccggaac tcgttgtgcc gtggatgacg acttcgtagg ggttgcagac taacatggcg
     2881 gcttcctgaa aagaaatgtc tggcgcgatt ataccttatg cttatgcggg cgtgtttgga
     2941 tatgccgtct gaaaagtacg ggattcgtgc ggtaaaactt tgcggcggca aatgtgcgat
     3001 aatacgcgcc gtattgccgc ttttgcgaag ctgttccgca aacatacggg cggcgtggac
     3061 gacgtataac cggatacccg cctgacgcgg gttttttacg gaaggggggc aaaaatgcct
     3121 aatccgcttt acagacagca tatcatctcc atttcggatt tgtcgcgcga acagttggaa
     3181 tgcctgcttc agacggcatt gaagctgaag gcgcatccgc gcggcgattt gttggaaggc
     3241 aaacttatcg gttcgtgctt ctttgaaccg tccacgcgca cgaggctgtc gtttgaaacg
     3301 gcggtgcagc ggctgggcgg caaggtcatc ggcttttcgg acggcgcgaa taccagtgcc
     3361 aaaaaaggcg agacgcttgc cgataccgcc cgcatcattt ccggctacac cgacgcaatc
     3421 atccaacgcc accccaaaga cggcgcggcg cgcgtggcag cggagttttc gcgcgtcccc
     3481 gtcatcaacg ccggcgacgg caccaaccag caccccagcc agaccttgtt ggatctggtt
     3541 accatttatg aaacgcaggg acgtttggac aagctcaaaa tcgccatggc gggcgacttg
     3601 aaatacggac gtaccgtgca ttcgctttgt caggcgttga aacgctgggg ctgcgaattt
     3661 gcctttgttt cgccgcccag cctcgcgatg cccgactaca ttaccgaaga gttggacgaa
     3721 gccggctgcc gataccgtat cctcggtagt ttggaagaag cggcggaatg ggcggacatc
     3781 ctgtatatga cccgtgttca acgcgaacgt ttcgacgaac aggaatttgc caaaatccaa
     3841 ggcaaattca atctcgacgc atccatgctt gcccgcgcca aaccgaacct gcgcgtgctg
     3901 caccctttgc cgcgcgtgga cgaaatccat cccgatgtcg atgctacgcc gcatgcctat
     3961 tatttcgagc aggcgaccaa cggcgtttat gcgcgtatgg cgatattgtc gctggtgttg
     4021 aacgaagaag tgtgaggaac cgatatggaa accccgaaac tcagtgtcga agccattgaa
     4081 aaaggtacgg ttatcgacca tattcccgcc ggcagggggc tgaccatcct gcgccagttc
     4141 aaacttttgc actacggcaa cgcggtaacc gtgggcttca acctgcccag caaaacccaa
     4201 ggcagcaaag acatcatcaa aatcaaaggc gtgtgcttgg acgacaaagc cgccgaccgc
     4261 ctcgccctgt tcgcccccga agcggtggtc aataccatcg acaatttcaa ggtcgtgcag
     4321 aagcggcatt tgaacctgcc cgatgaaatt gccgaagtgt tccgttgccc gaacacgaat
     4381 tgcgccggcc acggcgagcc ggtcaaaagc cggttttatg ttaaaaaaca caacgggcag
     4441 acgcggctga aatgccatta ctgcgaaaaa acctacagcc gggattcggt ggcggaagcc
     4501 tgacggattc ccttaaaccg agcgggcggc atttcgtctg ccgcttgttt tgccaatctg
     4561 aaatggaatg atgatgcacg cttctgtcca aagccgtttc gcaccgatac tttatgtttt
     4621 gattttcttt gccggttttt tgaccgcgca aatttggttc aatcagaaag cctatactga
     4681 agagctgcct ccgcttctgt ccgcattgtc cgccgtcgcg ctggtgtggc tggcgtgggc
     4741 gttcgtgtcg gcgcgttcaa aggctaaggc ggaaaagttc taccgcgaaa aaatgataca
     4801 gaacgaaagc atacaccccg tcctgcacgc ttctttgcaa cacttggaac acaagccgca
     4861 aatgctcgcc ctgctggtca aaaaccacgg caaagggatg gcggaacagg tcaggttcaa
     4921 ggcggaagtg ctgcccgacg acgaagacgc gcgcacgatt gccgccgagt tggcaaaaat
     4981 ggatatgttt gcattgggga cggacgcggt cgcctcgggc gaaacctatg gacgcgtgtt
     5041 cgccgatatt ttcgagttgt cggcggcttt ggaagggcgc gcgttcaaag gaatgttgaa
     5101 actgacggcg gaatataaaa acatcttcgg cgatgcctgc cgttcggaaa cggcgttgga
     5161 tttgggcgcg ctcaatcagg cgttgcagga gatttcaaaa acatcggaaa agtccaaacg
     5221 gatattttat tgaagatgga aaaatgccgt ctgaaacgga aggtgtttca gacggcattt
     5281 ttgtcggatg attaattatt cggagcggtc gaagccaaac ttcacgcggc tgcggccctg
     5341 atccggtata ttgtccaaat cgcgtcccgg attggcggcg gtgtcgccta cggaaatatc
     5401 ggagatgttt tccaaaatga tggcggacga caggtgttcg gaggtgcggt aaaccattgc
     5461 caagcccact tcttcggcag gagtggaaat cagctcgacg gtatccctgc ttttgaaatt
     5521 gttggagagg tcgacctgca tcgttttctt gcgtttgtag aggctcaaaa ccgtgccttt
     5581 gtccaaaccg tccgcctcgc ctttgtcgat ggtgatggtt tgaaactggc cggcaatcct
     5641 tgtaccttca aacacggaaa cgattttagc ctgaaccggg cgggacggtt cgtgcggcat
     5701 catgttgaag cggtcggtgt cttccggcat tttcatcagg tagtcgccct gctgtatttc
     5761 ggaaatggcg gtttcgacca ccagcggctg tatcgaaggg gtgcgcagcg gggtaatcaa
     5821 aggatgggtg cgggtatggt attcgttgtc tttcggccgt tctccagcct gtttcgagcg
     5881 ttgttcgagg acagagtcgg tatagtcgag ggagcgcacg atgccgctga atgcgacttc
     5941 ctgcccgagg aatttacccg tatccggatc ggtgatgttt ttattgattc ggtaggtcag
     6001 gtagcggccc ggctctttca ggcctttggt gtaaaccctg gtgcctttgg tgtacagcag
     6061 cctgccttcc gggcccgaga gcaggcgcgg cgcggcagcg gtttctttgc gggaaacgat
     6121 ttgcgggtgc cgcataaaga tgcggtagaa gttgacatcg atggcgggaa taccgtatcc
     6181 ggacacttcc ttatccggac tcattttgac gacggggatg ccgtctgtct gttccaagcc
     6241 gaggcgcggt tcgccgtcaa cgtggcgcaa caccaatacc tggtccggat aaatcaggtc
     6301 gggattgtgg atttgatccc ggttcgcgtc ccacaggcgg ccccattgcc acgggctgta
     6361 caggtatttg cccgaaatgc cccacagggt gtcgccctgt ttgaccgtgt agcgttccgg
     6421 cgcgttcggg cgcacctcca aatttgccgc caaagtttgt gttgagaatg ccatacctgc
     6481 cgcgcagagc agggttataa tacgacgttg cataaccgtt ccccttatct gataaatttc
     6541 ggtttgtctt gcttgattgg gttggaaaaa gcggcggcag cccctcggga tgtgccgcgt
     6601 gataaaaaat gttccgcatt ttaacatcga attatccgca ccatcacggt aattatgaaa
     6661 aacaggcggc gtatccgccg aaggaaagag aaaattatgg ctttattgaa tatcttgcaa
     6721 tatcccgacg agcgtctgca cacggtggca aagcctgtcg aacaagtcga cgagcgcatc
     6781 cggaagctga ttgccgatat gtttgaaacg atgtacgaat cgcgcggcat cgggctggcg
     6841 gcgacgcagg tcgatgtgca cgagcgcgtg gtcgtgatgg atttgaccga agaccgcagc
     6901 gaaccgcgcg tgttcatcaa ccccgtcatc gttgaaaaag acggcgaaac cacttacgaa
     6961 gagggctgcc tgtccgtgcc gggcatttac gacaccgtaa cccgcgccga acgcgtcaag
     7021 gtcgaggctt tgaacgaaaa aggcgaaaag ttcacgctgg aggcggacgg cttgctggcg
     7081 atttgcgtgc agcacgagtt ggaccacctg atgggcatcg tgtttgtcga acgcctgtcg
     7141 cagctcaagc agggacgcat caaaaccaag ctgaaaaaac gtcagaaaca tacgatttga
     7201 cccttttgcc gtgccgtctg aacgctgcaa agttttcaga cggcacggtc ttgtccgaca
     7261 accttacgca tacgcaggaa tatgctatga aagtcatctt tgccggcacg cccgattttg
     7321 ccgccgccgc cttaaaagcc gttgccgccg ccggttttga aatcccgctg gtgctgaccc
     7381 agcccgaccg tccgaaaggg cgcggtatgc aactgaccgc cccgcccgtc aaacaagccg
     7441 cgctggaact cggtttgcgc gtggcacagc ccgaaaaact gcgtaacaac gccgaagccc
     7501 tgcaaatgct caaagaggtc gaggcagacg taatggtggt tgccgcctac ggtttgattc
     7561 tgccgcagga agtgttggat acgccgaaac acggctgcct caacatccac gcttcgctgt
     7621 taccccgttg gcgtggcgcg gcgccgattc aacgcgcgat tgaagccggc gatgccgaga
     7681 caggcgtgtg tattatgcag atggacatcg gtttggacac cggcgatgtg gtcagcgaac
     7741 accgctacgc catccaaccg accgataccg ccaacgaagt ccacgacgcg ctgatggaaa
     7801 tcggtgcggc ggcggttgtt gccgatttgc aacagcttca aagcaaaggc cgtctgaacg
     7861 cggtcaaaca gcccgaagaa ggtgttactt acgcccaaaa actaagcaaa gaagaggcgc
     7921 gtatcgattg gagcgaaagc gcggcggtta tcgaacgcaa aatccgcgcc ttcaaccccg
     7981 tgcctgccgc gtgggttgag tatcagggca agccgatgaa aatccggcgg gcggaagtgg
     8041 tggcgcaaca aggcgcggca ggcgaagtgt tgtcctgttc ggcggacggt ttggtcgttg
     8101 cctgcggcga aaacgcgctg aagattaccg aattgcagcc tgccggcggc aggcgtatga
     8161 atatcgcggc gtttgcagca ggacggcata tcgaagcagg gacgaagctg taaatccctt
     8221 cagacggcat tccgatccgc aaacgggaat gccgcctgaa accatcagtt gaagaaagcg
     8281 aatcacataa tatgagtatg gcacttgccc aaaaacttgc cgccgacagc attgcggcgg
     8341 ttgccgaagg acgcaacctt caggacgtgt tggcgcaaat ccgcaccgcg catcccgacc
     8401 ttacggcaca ggaaaacggc gcgttgcagg acatcgccta cggctgccag cgttatttgg
     8461 gcagtttgaa acatatgctc gcgcagatgc tgaaaaagcc gattgacaac ccgcagctcg
     8521 aaagcctgct tttggcggcg ttgtaccaac tgcattacac gcgcaacgcg ccgcacgccg
     8581 tggtcaacga agcggtggaa agcatagcca aaatcggacg cgggcagtac cgttcgtttg
     8641 ccaacgcgat tttgcgccgc tttttgcgcg aacgcgacaa gcttgcggct tcctgtaaaa
     8701 aagacgatgt ggcgaaacac aacctgccgc tgtggtgggt ggcttacctg aaaaaccatt
     8761 atccgaaaca ctggcacaac atcaccgccg cgctacaatc ccatccgccg atgacgttgc
     8821 gcgtcaaccg ccgacacggc aatgccgaaa gctatttgga aaaactggct gcggaaggta
     8881 ttccggccag ggcgttggac gaatatgcgg ttacgttgga agaagccgtg ccggtaaacc
     8941 gtctgcccgg tttttcagac ggcattgttt cggtacagga cttcggtgcg cagcaggcgg
     9001 cgtatcttct taacccgaaa gacggcgaac ggattttgga cgcgtgcgcc gcgccgggcg
     9061 gcaagacggg gcatatcttg gaactggcgg attgccgcgt taccgccttg gacatcgatg
     9121 caggccgtct gaagcgtgtg gaggacaata tcgcccgttt gggctttcag acggcatcgg
     9181 cggcgtgtgc cgatgcgcgg gatttggcgg cgtggtatga tggaaagcct ttcgacgcaa
     9241 tccttgccga cgtgccatgc accgcttccg gcgtggcgcg gcgcaatccc gacgtgaaat
     9301 ggctgcgccg tccgaccgac gcgctcaaaa ccgcccgcca gcaggaagcc ctgctggacg
     9361 cactttggca gacgctgaaa cagggcggca gaatgctgct tgccacctgt tccgtgttcg
     9421 tcgaagaaaa cgacgggcaa ttgcaaaaat tcctcaaccg ccatgccgat gccgaaccga
     9481 tcgaatcgcg ggtgctttta ccgaacaaac accaagatgg cttttattac gcgcttattc
     9541 aaaagcatta aacaatggct tgtgctgctg ccgatgcttt ccgttttgcc ggacgcggcg
     9601 gcggagggga tagatgtgag ccgcgccgaa gcgaggataa ccgacggcgg gcagctttcc
     9661 atcagcagcc gcttccaaac cgagctgccc gaccagctcc aacaggcgtt gcgccggggc
     9721 gtgccgctca actttacctt aagctggcag ctttccgccc cgataatcgc ttcttatcgg
     9781 tttaaattgg ggcaactgat tggcgatgac gacaatattg actacaaact gagtttccat
     9841 ccgctgacca accgctaccg cgttaccgtc ggcgcgtttt cgacagacta cgacaccttg
     9901 gatgcggcat tgcgcgcgac cggcgcggtt gccaactgga aagtcctgaa caaaggcgcg
     9961 ctgtccggtg cggaagcagg ggaaaccaag gcggaaatcc gcctgacgct gtccacttca
    10021 aaactgccca agccatttca aatcaatgca ttgacttctc aaaactggca tttggattcg
    10081 ggttggaaac ctctaaacat catcgggaac aaataatgcg ccgttttcta ccgatcgcag
    10141 ccatatgcgc cgtcgtcctg ttgtacggac tgacggcggc aaccggcagc accagttcgc
    10201 tggcggatta tttctggtgg atagttgcgt tcagcgcaat gctgctgctg gtgttgtccg
    10261 ccgttttggc acgttatgtc atattgctgt tgaaagacag gcgcgacggc gtattcggtt
    10321 cgcagattgc caaacgcctt tctgggatgt ttacgctggt tgccgtactg cccggcgtgt
    10381 ttctgttcgg cgtttccgca cagttcatca acggtacgat taattcgtgg ttcggcaacg
    10441 atacccacga ggcgcttgaa cgcagcctca atttgagcaa gtccgcattg aatctggcgg
    10501 cagacaacgc ccttggcaac gccatccccg tgcagataga cctcatcagc accgcctccc
    10561 tatccggcaa tatgggcagg gtgctggaac attatgccaa cgggtttaca cagttgtccc
    10621 tttacaatgc cgcaagcggc aaaatcgaaa aaagcatcaa cccgcacaag ctcgatcagc
    10681 cgtttccgga taaggcgcgt tgggaaaaaa tccaacaggc gggttcggtc agggatttgg
    10741 aaagcatagg cggcgtattg tacgcgcagg gctggctgtc ggcgggtacg cacaacgggc
    10801 gcgattacgc cttgtttttc cgtcagccgg ttcccaaagg cgtggcagag gatgccgtct
    10861 taatcgaaaa ggcaagggcg aaatatgctg agttgagtta cagcaaaaaa ggtttgcaga
    10921 cctttttcct ggcaaccctg ctgattgcct cgctgctgtc gatttttctt gcactggtca
    10981 tggcactgta tttcgcccgc cgtttcgtcg aacccgtcct atcgcttgcc gagggggcaa
    11041 aggcggtggc gcaaggcgat ttcagccaga cgcgccccgt gttgcgcaac gacgagttcg
    11101 gacgcttgac caagttgttc aaccacatga ccgagcagct ttccatcgcc aaagaagcag
    11161 acgagcgcaa ccgccagcgc gaggaagccg ccagacatta tcttgaatgc gtgttggagg
    11221 ggctgaccac gggcgtggtg gtgtttgacg aacaaggctg tctgaaaacc ttcaacaaag
    11281 cggcggaaca gattttgggg atgccgctta cccccctgtg gggcagcagc cggcacggtt
    11341 ggcacggcgt ttcggcgcag cagtccctgc ttgccgaagt gtttgccgcc atcggcgcgg
    11401 cggcaggtac ggacaaaccg gtccatgtga aatatgccgc gccggacgat gccaaaatcc
    11461 tgctgggcaa ggcaaccgtc ctgcccgaag acaacggcaa cggcgtggta atggtgattg
    11521 acgacatcac cgttttgata cacgcgcaaa aagaagccgc gtggggcgaa gtggccaaac
    11581 ggctggcaca cgaaatccgc aatccgctca cgcccatcca gctttccgcc gaacggctgg
    11641 cgtggaaatt gggcgggaag ctggatgagc aggatgcgca aatcctgacg cgttcgaccg
    11701 acaccatcgt caaacaggtg gcggcattga aggaaatggt cgaagcattc cgcaattatg
    11761 cgcgttcccc ttcgctcaaa ttggaaaatc aggatttgaa cgccttaatc ggcgatgtgt
    11821 tggcattgta tgaagccggt ccgtgccggt ttgcggcgga gcttgccggc gaaccgctga
    11881 tggtggcggc ggatacgacc gccatgcggc aggtgctgca caatattttc aaaaatgccg
    11941 ccgaagcggc ggaagaagcc gatgtacccg aagtcagggt aaaatcggaa gcggggcagg
    12001 acggtcggat tgtcctgacg gtttgcgaca acggcaaagg gttcggcaag gaaatgctgc
    12061 acaacgcctt cgagccgtat gtaacggaca aaccggcggg aacgggattg ggtctgcctg
    12121 tggtgaaaaa aatcattgaa gaacacggcg gccgcatcag cctgagcaat caggatgcgg
    12181 gtggcgcgtg tgtcagaatc atcttgccaa aaacggtaaa aacttatgcg tagcagcgat
    12241 attttaattg tagacgacga aatcggcatc cgcgacctgc tgtcggaaat cctgcaggac
    12301 gaaggttatt cggtcgcatt ggcggaaaac gccgaagagg cgcgcaagct gcgccatcag
    12361 gtacgcccgg cgatggtgtt gctggatatt tggatgcccg attgcgacgg catcaccctt
    12421 ttgaaggagt gggcgaaaaa cgggcagctc aatatgccgg tggtgatgat gagcgggcat
    12481 gccagcatcg ataccgccgt agaagcgacc aaaatcggcg cgctcgactt tttggaaaaa
    12541 ccgatttccc tgcaaaagct gctgtctgcc gtcgaaaacg cgttgaaaca cggtgcggcg
    12601 aaaatcgaaa cggggcctgt attcgacaag ctgggcaaca gtgcggcgat tcaggagatg
    12661 aactgcgaag tcgaagcggc ggcaaagcgc acgtcccctg tatttttgac gggcgaagcg
    12721 ggttcgccgt ttgaaaccgt ggcgcgctat ttccataaaa acggtacgcc gtgggtcagt
    12781 ccggcaaggg tcgaatatct gatcgatatg ccgatggaac tgttgcagaa ggcggagggc
    12841 ggcgttttgt atgtcggcga catcgcccaa tacagccgca atatccaaac cggcattgcc
    12901 tttatcatcg gcaaggcaga acgctgccgc gtcagggtgg tcgcatcggg cagcagggcg
    12961 gcaggttcag acggcattgc ctgcgaggaa aagctggcgg aactgctgtc ggaatcggtc
    13021 gtccgtattc cgccgctgcg tatgcagcat gaagatattc ccttcctgat acaggggatt
    13081 gcctgcaatg tggcggaaag ccaaaagatt gcgcctgcct cattcagtga agaggcactt
    13141 gccgcattga cccgttacga ctggccggga aatttcgacc aactgcaaag cgtcgttgca
    13201 acgctgttgt tggaggcgga cggacaggaa atcggcgcgg cggcggtttc ttcccttttg
    13261 gggcagaatg tgcctgccga gggagcggaa gatatggtgg gcgggtttaa tttcaacctg
    13321 cccctgcgcg aattgaggga ggaggtggag cggcgttatt tcgagtacca catcgcccaa
    13381 gaaggtcaga atatgagcaa agtggcgcag aaggtcggtt tggaacgcac gcacctttac
    13441 cgcaaactca aacagctcgg catcggcgtt tcgcgccggg cgggggaaaa aaccgaagaa
    13501 taggcccgga cggccggttt tccggctgcg ggcttttgtt ttcagacggc atttggtgca
    13561 aatgccgtct gaaatcgtaa ggggacggat tttatgacag aggacgaacg tttcgcgtgg
    13621 ctgcaattgg cgtttacgcc ctatatcggc gcggaaagct tcctgctgct gctgcgccgt
    13681 ttcggcagcg cgtcaaacgc cctgtccgca ccggcggaac aggtcgcccc ggcggtgcgg
    13741 cacaagcagg cggccgaggc ttggcgcaat gcggaaaaac gcgcctcggc gcggcaggcg
    13801 gcagaagcgg cgttggaatg ggaaatgcgg gacggatgcc gtctgatgct gcttcaggat
    13861 gaagatttcc ccgaaatgct gacgcagggg ctgaccgcgc cgccggtttt gtttttgcgc
    13921 ggcaacgtgc ggctgctgca caagccttcc gccgccatcg tcggcagccg ccatgccacg
    13981 ccgcaggcga tgcggattgc caaagatttc ggcagggcgt tgggtgggaa aggcattccc
    14041 gtcgtgtcgg gtatggcttc gggcatcgac accgccgccc atcagggcgc gttgcaggca
    14101 gaaggcggca ccatcgccgt gtgggggacg ggcatagacc gcatttatcc gccgtccaat
    14161 aaaaaccttg cttatgaaat tgccgaaagg ggattgattg tcagcgagtt ccctttggac
    14221 acgcgtccgt ttgcaggcaa ctttccgcgc cgcaaccgcc tgattgccgc actgtcgcaa
    14281 gtaacgctgg tggtcgaggc cgccttggaa tccggttcgc tgattaccgc cggtttggcg
    14341 gcagaaatgg ggcgcgaggt aatggcggta cccggttcga tagacaaccc gcacagcaaa
    14401 ggctgccaca aactgattaa agacggcgcg aaactgacgg aatgcctgga cgacatcctg
    14461 aacgaatgcc cgcggctctt gcaaaatgag ggtgcttcgt catattctat aaataaggga
    14521 atgcctgaaa agcgcatcac tgccgttcag acggcatccg accagctgtc tctgcctgaa
    14581 ggcaaaatgc cgtctgaaaa gacggagaac cgacccgtcg gcggcagtat cttggacagg
    14641 atgggtttcg acccagttca tcccgacgtg cttgccggac agttggctat gcctgccgca
    14701 gatttgtatg ccgcactgtt ggaattggaa ttggacggca gcgttgccgc aatgcccggc
    14761 ggcagatacc agcgtatccg aacttgaacg cactttatat taaggaacac gaatgaccga
    14821 agtcatcgcc tacctcatcg aacatttcca agatttcgat acctgtccgc cgcccgaaga
    14881 cttgggtatg ctgcttgaag aagcgggttt cgatacgatg gaaatcggca acaccctgat
    14941 gatgatggaa gtattgctca acagctccga attttccgcc gaacccgccg acagcggcgc
    15001 attgcgcgtg tacagcaaag aagaaaccga caacctgccg caggaagtga tggggctgat
    15061 gcagtatctg attgaagaaa aagccgtcag ctgcgaacag cgggaaatca tcatccacgc
    15121 gctcatgcac attccgggcg acgaaattac cgtagatacc gccaaagtgc tgaccctgct
    15181 gcttttatgg gcaaacaaga gcgagctgcc cgtgttggtc ggcgacgagc tgatgagcgc
    15241 gcttttactc gacaacaaac ccacgatgaa ctgaagcggc ttcagacggc ccgcccgagg
    15301 ccgtctgaaa cgccggcatc aaaaccacca tccagagaac gacaaatggc gaaaaaccta
    15361 ttaatcgtcg aatccccgtc caaagccaaa accctgaaaa aatatttggg cggcgatttt
    15421 gaaatccttg catcctacgg acacgtccgc gacctcgtcc ccaaaagcgg cgcggtcgat
    15481 cccgacaacg gctttgcgat gaaataccaa ctcatcagcc gcaacggcaa acacgtcgat
    15541 gccatcgtcg ccggtgccaa agaagctgaa aacatctacc tcgccaccga cccggatagg
    15601 gaaggcgaag ccatttcctg gcatcttttg gaaatcctca aatccaaacg cggcttgaaa
    15661 aacatcaagc cgcagcgtgt cgtgttccac gaaatcacca aaaacgccgt gctcgatgcc
    15721 gttgcccatc cgcgcgaaat cgaaatggac ttggtcgatg cgcaacaagc ccgccgtgct
    15781 ttggactatt tggtcggctt taacctttcg ccattgttat ggaaaaaaat ccgccgaggt
    15841 ttgagcgcgg ggcgtgtgca aagccctgct ttgcgcctga tttgtgagcg cgaaaacgag
    15901 atccgcgcgt ttgaagcgca ggaatattgg acggtacatc ttgacagtca caaaggacgc
    15961 agcaagttca ccgccaaact cgcccaatac aacggcgcga aactcgaaca attcgacctg
    16021 ccgaacgaag ccgcgcaagc cgacgtgttg aaagaactcg aaggcaaaga ggccgtcgtt
    16081 accgccattg aaaagaaaaa gcgcagccgc aatcctgccg cgccgttcac cacttctacc
    16141 atgcagcagg atgccgtgcg caaactcggc ttcaccaccg accgcaccat gcgtaccgcc
    16201 caacagcttt atgaaggtat agacgtaggg cagggcgcga tcggtctgat tacctatatg
    16261 cgtactgaca gcgtgaattt ggcggatgaa gcgttaaccg aaatccgcca ttacatcgaa
    16321 aacaaaatcg gcaaagaata tctgccgagt gccgccaaac aatacaaaac caaatccaaa
    16381 aacgcccaag aagcgcacga agccatccgt ccgacttccg tgtaccgcac gcccgaaagc
    16441 gtcaaaccct tcctgagcgc agaccagttc aaactctatc aaatgatttg gcagcgtacc
    16501 gtcgcctgtc agatgacgcc tgccaaattc gaccaaacca ccgtcgatat taccgtcggc
    16561 aaaggcgtat tccgcgtaac cggacaagtg caaaccttcg caggcttctt gagcgtttat
    16621 gaagaaagca gcgacgatga agaaggcgaa gacagcaaaa aactgcccga aatgagcgaa
    16681 ggcgataaat tgcccgtgga caaactctac ggcgaacaac actttaccac tccgccgcca
    16741 cgctacaacg aagccacgct ggttaaagcc ctcgaagaat acggcatcgg ccgcccctcg
    16801 acctacgcca gcatcatctc cacgctcaaa gaccgcgaat acgttaccct tgagcaaaaa
    16861 cgcttcatgc ccaccgacac aggcgacatc gtcaacaaat tcctgaccga acacttcgcc
    16921 caatacgtcg attaccactt taccgccaaa ctcgaagacc agcttgacga aatcgccggc
    16981 ggcaaacgcc aatggattcc cttgatggac aaattctgga aaccgttcat caaacaagtg
    17041 gaagaaaaag aaggcatcga acgcgccaaa tttaccacgc aggaacttga tgaaacctgc
    17101 ccgaaatgcg gcgaacacaa actgcaaatc aagttcggca aaatgggccg tttcgttgcg
    17161 tgtgccggct atcccgagtg cagctacacg cgcaatgtca atgaaaccgc cgaagaagcc
    17221 gcagagcgca tcgccaaagc cgaagccgaa caggcagaac tcgacggacg cgaatgtcct
    17281 aaatgtggtg gacggctggt gtacaaatac agccgtaccg gcagtaaatt catcggttgc
    17341 gccaactacc ccaaatgcaa acacgtcgag ccgctggaaa aaccgaaaga taccggcgtc
    17401 cagtgtccgc aatgtaaaaa aggcaacctc gtcgagcgta aatcccgcta cggcaaactg
    17461 ttttacagtt gcagcaccta tcccgactgt aactacgcca catggaaccc acccgttgca
    17521 gaaagctgtc caaaatgcgc atggccggtt ttgaccatca aaaccaccaa acgctggggt
    17581 gtagaaaaag tctgcccaca aaaagaatgc ggctggaaag aacagattga accgcccgcg
    17641 ccgaaggagt aagattaggt tggtttgaaa gagaaaaggt cgtctgaaaa attttcagac
    17701 gacctttgct tttctgtgat tggtttattt gaatccgcgt gttgttttaa agtccgataa
    17761 aatccggttc atttcaggcg caaacaaggc gatgtaatcg taagatagac cgcgactggc
    17821 actgggatgg ggaaagcaga cgacttcgca atcttcaaac gattggaatt tgacattgaa
    17881 acgtgtaccg tcaaattctt tttgcaccgt ctccagcggt ttggtctgct taccgaccaa
    17941 ctgctcgaag cgtggcagta cattttggtt gttcagaaaa tccgccaacc tgctgcccat
    18001 gaagaggatg actttcggac gcagtttttc gatgtggtag agaaaattat cgatgtgctc
    18061 gggttgtgtg aacttgtctg gattgtcgat agtgttgccc tgtgtagcag cccagttggt
    18121 ttgaaccagg gatttttcaa atgcaccgcc caatccattt tcgtctaagg ggtgtcccca
    18181 catttcaaac caatttttta tcgtattatc gtaacgccac ttttttgcct gctctccgaa
    18241 atagagggat ttgtttgcaa atgtatggtc gattttgttt tcagggagtt tgtattcacc
    18301 tgctacataa gcagcctcat cggctttact ccaaccccat tcatagccac aaatcattaa
    18361 gccatgtttg tcgttgtagc ctttgaacag gctgttgctc aaattcaaat ccttcatcat
    18421 gaactcttcc ttttaaaatt taagagcgat tgacttcaat gtttttagat ggggtggaaa
    18481 aatccttgtg taggcaacat aaattcaata aatttcttga taattcgaaa cctactaata
    18541 gcgcacctat aaaagctttt tcattacgtt cagcatgacg gtcacgtcgt tcatattttt
    18601 tacgcttgct gttccctgtt attacagcta agccaagtga tatggcgaga attgcccaaa
    18661 caatagtact taaataacaa ttttccccat actatcaata aggaaagaaa aaacctttta
    18721 gtattaggtc gatagattat aatccatgcc catgaaaatg caataagagt tatacataag
    18781 atacaagctg acaggatttc tttattttta attaaataac ttagagcgat gaggatgaca
    18841 ggtgtcaaga aaataatagt tacatcccga tagctataaa agaaaactgc cctattttga
    18901 atgtggagat gtgcacagaa tccaatatag ctaaggataa tagttaatat aataaaaaaa
    18961 gaccaccaag ggtgaagaga taggaattcc atgttttccc tttttttgta aaaaggaaaa
    19021 aatcttatct aatagttaat tgcctaacca gccagaagaa gtttaaaatc tatcccaata
    19081 attcaaccat ctatacagaa agttcagctt atggaaaccc acgaaaaaat ccgcctgatg
    19141 cgcgaattga ataaatggtc ccaggaggat atggcggaaa agctggcgat gtcggcagga
    19201 gggtatgcca aaatcgaacg gggcgaaacg cagttgaatg tcccgcgttt ggagcagttg
    19261 gctcagattt tcaaaatcga tatgtgggac ttgctcaaat cgggcggtgg tgggatggtg
    19321 tttcagatta atgaaggtga tagtggtggc gatattgcgt tgtatgcgtc gggtgatgtt
    19381 tcgatgaaaa tagaattttt aaaaatggag ttgaaacact gcaaagaaat gttggaacaa
    19441 aaagacaaag aaatcgagct gctccgcaag ctgaccgaaa ccgtttaaac agatatgccg
    19501 tctgaaaaaa gttttcagac ggcatattct ttgacaggtc ttgtataata ccgtttgaac
    19561 ttacaggttt ttgattatgg cggcaggcaa acataccaaa cacagcaacc gggtacgcat
    19621 tatcggcggg caatgccggg gcaggaaatt gagtttcaca tccgccgacg gactgcgtcc
    19681 gacacccgac agcgtgcgtg aaaagctgtt taactggctg ggacaggatt tgacgggtaa
    19741 aacggttttg gatctcttcg gaggcagcgg cgcactcggt atagaagccg cttcgcgcaa
    19801 cgccaaacgc gtgctgattt cggataacaa ccgccaaacc gtgcagacct tgcagaaaaa
    19861 cagtcgcgaa ctgggtttgg ggcaggtgca aatcgtctgt tcagacggca tcgcatattt
    19921 gaagaccgta tccgaacagt ttgatgttgt ctttctcgac ccgccgtttg catggcagga
    19981 ctggcaaatc ctgtttgatg ccttgaagcc gtgcctgaac ccccgagcat tcgtctatct
    20041 cgaggcgggt acgctgccgg atattcccga ttggctgacg gaatatagag aagggaaatc
    20101 ggggcagagt acattcgaat taagggtttt ccaagtggct gaataatatg cgctttgata
    20161 atcatttccg agttgtaaac attcgtttgc aaccgtccgg ttcaaaaaaa ccttgtgcta
    20221 taatccgcgc ccgcccggtt ttgataattt agtggaaaag gaaaagaaat gtcgcttttt
    20281 attaccgacg agtgcatcaa ctgcgacgta tgcgaacccg aatgccccaa tgatgccatt
    20341 tcccaaggcg aggaaattta cgaaatcaac cccaacctct gcacgcagtg cgtcggacac
    20401 tacgatgagc cgcagtgcca gcaggtttgc ccggtggact gcatcctgat tgacgaagaa
    20461 catcccgaaa cccatgacga gttgatggcg aaatacgaaa agattatcca gtttaaataa
    20521 attcttttta aaacatcaaa ttatgtctgt tttgaaataa aatcaaaaaa aaacttgacg
    20581 gaaaagcaag ccgctaataa actaacgttc tcttttggag ggattcccga gcggtcaaag
    20641 ggggcagact gtaaatctgt tgcgaaagct tcgaaggttc gaatccttct ccctccacca
    20701 aaattcttac ttggggcagt agcgagtaat gcgggtgtag ctcaatggta gagcagaagc
    20761 cttccaagct tacggtgagg gttcgattcc cttcacccgc tccaaacaat taggcccatg
    20821 tagctcaggg gtagagcact cccttggtaa gggagaggtc ggcagttcaa atctgcccat
    20881 gggcaccatc tctcgattat tcatttcttt aaggcttaga tatataggat attg
//
LOCUS       DAWEWP010000019        20679 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWEWP010000019 DAWEWP010000000
VERSION     DAWEWP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.019"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(63..3668)
                     /gene="putA"
                     /locus_tag="WGN53_000511"
     CDS             complement(63..3668)
                     /gene="putA"
                     /locus_tag="WGN53_000511"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ0139537.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHCRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDCLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDTKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRNDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     SVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
     gene            3660..3827
                     /locus_tag="WGN53_000512"
     CDS             3660..3827
                     /locus_tag="WGN53_000512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139538.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            complement(3917..5443)
                     /gene="putP"
                     /locus_tag="WGN53_000513"
     CDS             complement(3917..5443)
                     /gene="putP"
                     /locus_tag="WGN53_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ0139539.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            5926..6246
                     /locus_tag="WGN53_000514"
     CDS             5926..6246
                     /locus_tag="WGN53_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ0139540.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            6260..7756
                     /locus_tag="WGN53_000515"
     CDS             6260..7756
                     /locus_tag="WGN53_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ0139541.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            7862..8707
                     /gene="ftsN"
                     /locus_tag="WGN53_000516"
     CDS             7862..8707
                     /gene="ftsN"
                     /locus_tag="WGN53_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0139542.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREKSDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKAPKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            8710..9354
                     /locus_tag="WGN53_000517"
     CDS             8710..9354
                     /locus_tag="WGN53_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0139543.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQKQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVKLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAEKWALSQKGFDGKKLMRAYDSPAAAAAASKMQQ
                     LTEQYRIDSTPTVVVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            9387..10208
                     /locus_tag="WGN53_000518"
     CDS             9387..10208
                     /locus_tag="WGN53_000518"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ0139544.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEITIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIVDVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(10490..10565)
                     /locus_tag="WGN53_000519"
     tRNA            complement(10490..10565)
                     /locus_tag="WGN53_000519"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10530..10532),aa:Ala,seq:ggc)
     gene            complement(10573..10649)
                     /locus_tag="WGN53_000520"
     tRNA            complement(10573..10649)
                     /locus_tag="WGN53_000520"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10613..10615),aa:Met,seq:cat)
     gene            complement(10781..11998)
                     /locus_tag="WGN53_000521"
     CDS             complement(10781..11998)
                     /locus_tag="WGN53_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0139545.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEEIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYL
                     PQSHFLLNLGITELLAQVGKTDSPAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVD
                     WAGFRFGDICHKL"
     gene            12277..12732
                     /gene="mraZ"
                     /locus_tag="WGN53_000522"
     CDS             12277..12732
                     /gene="mraZ"
                     /locus_tag="WGN53_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ0139546.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            12729..13715
                     /gene="rsmH"
                     /locus_tag="WGN53_000523"
     CDS             12729..13715
                     /gene="rsmH"
                     /locus_tag="WGN53_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ0139547.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQAALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            13712..13975
                     /gene="ftsL"
                     /locus_tag="WGN53_000524"
     CDS             13712..13975
                     /gene="ftsL"
                     /locus_tag="WGN53_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ0139548.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            14036..15781
                     /locus_tag="WGN53_000525"
     CDS             14036..15781
                     /locus_tag="WGN53_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ0139549.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            15806..17284
                     /locus_tag="WGN53_000526"
     CDS             15806..17284
                     /locus_tag="WGN53_000526"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ0139550.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDGYGAELIGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFRTPWGEGKCRTRLLGRFNAQNLAACIALLCANDYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAVRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            17307..18953
                     /locus_tag="WGN53_000527"
     CDS             17307..18953
                     /locus_tag="WGN53_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ0139551.1"
                     /translation="MKQSARIKNMNQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            19079..20437
                     /locus_tag="WGN53_000528"
     CDS             19079..20437
                     /locus_tag="WGN53_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ0139552.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDRVAMDGALKVDDTLAALQTLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGVDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            20440..20616
                     /locus_tag="WGN53_000529"
     CDS             20440..20616
                     /locus_tag="WGN53_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139553.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
BASE COUNT         4793 a         5058 c         5996 g         4832 t
ORIGIN      
        1 gccgtctgaa aggttttcag acggcattgg ttcacggccc gcgcccgggg atttcggtaa
       61 aatcagtcgg cgaccgccat caggctggcg ttgccgccgg cggctgtggt gttgacgctg
      121 caagagattt cttcaaacac ttgcaggatg tcgagtccgt tttccgaagg gaggatgcgg
      181 atgagtgcgc cgtcgtgggc ggcaagttcc tgtttgcgcg cgctgtccaa aggcgacagg
      241 gcggcaacgt ggctgatgtc ggcggtttcg ggtttgccgt tgaccagcag cagaccttcc
      301 aagtcggcgg tgtaggaagc cagcgggctg tcgggttcga ccactgcctg tacgccggag
      361 gcggcaagtt cggttagggc gcagaaggct tgcaggaggc tgccgccgtg tatccaaacg
      421 cgtttgggcg cgtgccatga gatgctgttg cgctcgccgg tcggtccggt aaggacggtt
      481 tcggcacggc gcagggtgcg gatgcgggcg tgtcccaaag cggccgctgc ggcttttttc
      541 tcttcggcgt tgaacggtag tttgtgaacc agtgcttcga ggcgtttgag tgcggcttcg
      601 tccgcctgtc cgatttggct cagggtcggg gcaacccatt cgccggcgcg ggtcagtttt
      661 tgcaggtaga atgaaccgcc tgctttggga cctgtgccgg acagaccgtg tccgccgaag
      721 ggctgtacgc cgacgactgc gccgacgatg ttgcggttga cgtaaacgtt gccggcttcg
      781 atgcggctgc ggatgtggcg taccgtgcct tcgatgcggc tgtgtacgcc gtgggtcagg
      841 gcgtagcctt tgctgttgat ttggtcgatg acgctgtcga gttcgtcggc gcggtagcgg
      901 acgacgtgca ggacgggacc gaagacttcg cgttgcagtt cgttgaggtt gttcaattca
      961 aacaggatgg ggcggacgaa cgtggatttt tcggaatcga catcggcagc ggttttgact
     1021 tcgtggtaag acttggcaac gcctttcatt ttgttgatgt ggttcaacag gttttgctgc
     1081 gcttcggcat cgatgacgga gccgacatcg gtggtgagtt gaatcggttt gccgacgacg
     1141 agttcgtcca tcgcgccttt gatcatgtcg agcatacggt cggcaacgtc ttcttggacg
     1201 cacaagatac gcagggcgga gcagcgttgt cccgcgctgt cgaaggcgga gttcaatacg
     1261 tcggcgcaga cttgttcggc aagtgcggtg gaatcgacaa tcatggcgtt ttgtccgccg
     1321 gtttcggcaa tcaggacggg attgtcgttg cgtttggcaa gggctttgtt gatcaggcgc
     1381 gccacttcgg tcgagccggt gaaaattacg ccgccgatgc gggcatcgtt ggtcaatgcc
     1441 gcacccacgt cgcctgcgcc gaggacgagt tgcagggcgg aagtcgggat gccggcttcg
     1501 tgcatgaggg aaacggcata accggcaatc aggctggttt gttcggcggg tttggcgata
     1561 acggtgttgc ctgccgccaa tgcggacacg acttcgccgg taaagatggc gagcgggaag
     1621 ttccacgggc tgatggcgac aatcgcgccg acggcttttg tgtcttgagg cagggtgtgt
     1681 tcggcttcgt ttgcgtagta gcggcagaaa tcgacggctt cgcgcacttc ggcaatggcg
     1741 ttgttcagcg ttttgcccgc ttcgcgcacg gcaagcatca ttagcgcggg ggtgtgctgt
     1801 tcgagcaggt cggcgaaacg gcgcaggcag gcggcgcgtt cggcggcagg tgtcgcactc
     1861 cattcgggga acgcggcaac ggctgcgcca accgcttctt gggcaagcgc ggcatcggca
     1921 aagctgactg tgccgacgat gtcgtcgtgg tcggcaggat ttttaatcgg ttgcgcttcg
     1981 ccgacatcgc gggctttgcc gttgacgatg gatgcggcgt ggaagtcttg tgcggcggct
     2041 ttgttcatct gttcttgaag ctgctgcaat acgttttcgt tgctcaagtc cacgccttgc
     2101 gagttcagac ggcatttgcc gtacaaatcg cgcggcagcg gcagggcgtt gtgcaggtgg
     2161 atgccttgtt cggcgatggt gtcgaacggg ctcttaatca ggcagtcgat gctgatgttt
     2221 tcatcgacga tttggttgac gaaagacgag ttcgcgccgt tttccaacag gcggcgcacc
     2281 aagtaggcga gcagggtttc gtgtgtgccg accggggcgt acacgcgcac gcggcggcct
     2341 aagttttgcg ggccgacgac ttggtcgtac agggtttcgc ccatgccgtg caggcattgg
     2401 tgttcaaagt ctttgccttt gcccatttgg tagattgcgc ctaaggtgta ggcgttgtgc
     2461 gtggcaaatt gcgggaatac cgcgtcttgc gcggaaagca gtttgcgcgc gcaggcgagg
     2521 taggagatgt cggtgtggac tttgcgggtg taggtcggat agccgttcaa gccgtccact
     2581 tgcgcccatt tgatttcgct gtcccaatac gcgcctttga cgaggcggat catcagtttt
     2641 tggttgttgc ggcgggctag gtcaatcagg tagtcgataa cgaacggaca acgtttttgg
     2701 taggcttgga caacgaaacc gatacctttg tagccagcca agtcagggtc tgaaaccaaa
     2761 gcctccatca aatccaaaga cagctccaag cggttggctt cttcggcatc gatgttgata
     2821 ccgatatcgt attttttgcc caaaaggaac agctctttca ggcgcggcaa cagttcgccc
     2881 atcacgcggc agtgttgggc gcgcgagtag cgcggatgga tggcggagag tttgacggaa
     2941 atgccgttgc cttcgtaaac gccttgtcct gccgcatctt tgccgatggc gtggatggct
     3001 tcgacatagt cgcggtagta gcggtcggca tcggcttggg tgtaggcggc ttcgcccaac
     3061 atatcgaagg agaagcggta gcccattttt tcgcgttctt tgccgttttg cagggcttct
     3121 tcaatggtct gtccggtgac gaactgtttg cccaacaggc gcatggcgta attcacgcct
     3181 tggcggatga gcggtgcgcc gcctttgctg atcaggcggc tgagtgcgga actcatttgt
     3241 ttgtcgtttg tggcggtcag tttgccggta atcagcaggc cccaggcggc agcattgacg
     3301 aagagggaag ggctgttgtt caaatggctt ttccagttgc cgtctgaaat cttgtcggca
     3361 atcaggcggt cgcgcgtggc gttgtcgggg atacgcagca gggcttccgc cagacacatc
     3421 agtgcgatgc cttcttcgct ggagagtgag aattcgtgca tcagcgcatc cacgccgccg
     3481 gctttggtgc ggccggcgcg gacttgggta accaaacggc gggcaagctc ggaggcggcg
     3541 ttgcgctctt cgtcgctcat ctgtgcacgt tgcaacatat cctgtacggc ttcgatttca
     3601 ttacggcggt aggcatcggt tatcgcttgg cgcagggcag tttgtgccgg aaatgcaaaa
     3661 tgaaacattt tttggattct ccaaagtttt tcggggggca ggcggcatcg gtgcggcctg
     3721 aatacggtaa tatcgtaata aatccgcaga tgaaatacaa ggcttcaaat gcgggcaggg
     3781 taggtgcttc cgtttctttg aaaatgaaac gggtaaaaca caaataaggc ctgtatgcag
     3841 gcaaggttta tttgtgtttg acccggaaac gggttcagac ggcacgaacc gggatgccgt
     3901 gccgtctgaa aggggtttat cgggcggcgc ggtaatctgc gtcggctttt tcaaagcgtt
     3961 cttgggtttc gcgcgaaggt tctttgttga acagggaaac caatacggcg acgattaagc
     4021 aaacaataaa gcccgggacg atttcgtaca tcgtcaacaa gccgctttcg cctgccgctt
     4081 gagccggttt ttttacccat tccgcccata cgactacggt taaggcacct gcaaccatac
     4141 ccgacaacgc gccgtaggca gtgatgcgtt tccacaatac ggacagaatc acaatcgggc
     4201 cgaacgccgc accgaaacct gcccacgcat aagacaccag tcccaatact ttgctgttcg
     4261 gatcggaagc aatcaggatg gaaatcacgg caatcgccaa gaccatcagg cggccgaccc
     4321 ataccagttc cgactgttgc gcgtttttac gcaaaaagcc tttgtagaag tcttcggtaa
     4381 tcgcgctgga gcaaaccaaa agctggcagg acagggtgga catcaccgcc gccaaaatcg
     4441 cgctcaaaat gatgccggcg atccaagggt tgaacagcag ggtggaaagc gcgatgaaga
     4501 tgcgttcgtg gttgccgctc atagaagaaa ctttgtcggg atttgcaccg aaatacgcaa
     4561 tgccgaaata accgaccgcc accgcgcccg caaggcacaa cgccatccaa gtcatgccga
     4621 tgcggcgtgc ggataccagc gatttcgcgc tttcggccgc cataaagcgc gccaaaatgt
     4681 gcggctgtcc gaaatagccc aagccccatg cggcggtgga aatgatgccg atgacggtcg
     4741 taccggcaaa caggctgccg tattctttgc ccgtgcctgc ggcgacgctt tgaatcgcgg
     4801 cagacatctg ttccgcgccg cccaagccca gatagaccat caccggcgtt aaaatcaggg
     4861 cgaaaatcat caaagaagcc tgcagcgtat ccgtccagct gaccgccaag aagccgccca
     4921 agaaggtata ggcgatggtc gcgcccgcgc ccagccacat tgcctgattg taagtcatac
     4981 cttcaaacag gctttggaac agggttgcgc ccgccacaat gcccgaggcg caataaatcg
     5041 tgaagaagaa caggataatc agtgcggaaa ccactttcat caagtgtccg cccgcgccga
     5101 agcggtggaa gaaataatcc ggcagcgtca gcgcattgtt ggcgtattcg gtatgcacgc
     5161 gcaggcggcc cgccaccaag agccagttga aatacgcgcc gaccaagagg ccgatggcaa
     5221 tccaagcctc attcaaaccg ctcaggtaaa tcgcgcccgg cagacccatc agcagccagc
     5281 cggacatatc ggacgcgcct gccgacatcg cggtaacaaa cgggcctagg ctgcgcccgc
     5341 ccaaaatata atcgtcgaaa ttgcgcgtag aaaaataggc ggcaagcccg atgagaagga
     5401 ctgcaaccag atagattgca aaagtaatgt acatgggatt catgtgctat tcctcgtcta
     5461 aaacttcaga attacaggct ttgaaattgc aagcaacttg cgcctgaaat gtttttctaa
     5521 taaaagtaca acggaaaatc cggatacccg aaagggggat tcggataaat tatcttcaat
     5581 cacaataaga tatgtaataa aactatatga aattgtaaat aatccgtttc agcataaccc
     5641 aatttctgtt gtttgcaaag cacttaatgg cttaaaaagc cgagtttgaa acgatgcgcg
     5701 tcggaaaaat catttaaaac agcatattgt tttgtagtgt cttgtaatca ggcgttgcgc
     5761 ggaatatgaa atccgttttc aggcggcagg tgttttgagg tgtaatttag caaccgcaaa
     5821 ggaggcgcgg tatgttttgc cgattatccg ccgcccgttt tcagacggca tttttcctta
     5881 tacaatagcc gattgaattt gatatgttca ggaaggatac agattatgtt cggcaagcag
     5941 ctttttgagg aagtcggctc gaaaatcagc gaaaccatcg ccaacagccc tgccaaagat
     6001 gtggaaaaaa atattaaggc gatgctgggc ggcgcgttca accgtatgga tctggttacg
     6061 cgcgaagaat tcgacatcca gcagcaggtt ttaatcaaaa cccgtaccaa actggcggct
     6121 ttggaagcgc gtttggcaaa actcgaagcc gcgcaaaatc ccgaacgggc agcattggaa
     6181 gcggctgaag ccgctgccga agaagccgtc gccgaaatca ggcagcaaac cgaagccggc
     6241 gaataaggcc gtctgaaata tgtcgcttgc cttggtttac agccgcgcct tgagcggtat
     6301 gaatgcgccg ttggtcgaag tggaagccca ccttgccaac ggtctgccgc atttcaacat
     6361 cgtcgggctg cccgatacgg aagtcaaaga aagtcgcgac cgcgtccgtg ccgccattat
     6421 tcaaagcggt tttgaattcc ccgccaaaaa aatcaccgtc aacctcgccc ccgccgacct
     6481 gcccaaagag tcggggcgtt tcgatttgcc gattgcaatc ggcatccttg ccgcatcggg
     6541 gcaggttgcg cccgaaaaac tggcggaata cgagtttgcg ggggaattgg cactgtcggg
     6601 gctgttgcgc cccgtgcgcg gcgcgttggc gatggcgtgg cagggtatgc aggcaaaacg
     6661 tgcatttgtt ttgcctgaag aaaatgcagg acaagccgcc gtgatgcgcg gcattaccgt
     6721 ttacggcgcg cgctctttgg gcgaagtcgc cgcccatttg aacggcatcg aacctttggc
     6781 gcaaaccgaa tgccaagttc ctcagatgcc gtttgaacat ggcggacaac ctgatttgtg
     6841 cgatgtgaaa ggtcagcaca ccgcgcgcct tgctttggaa atcgctgccg caggcggaca
     6901 cagcctcttg atgatgggtc cgccgggaac gggcaagtct atgctctccc aacggctgcc
     6961 cggcatcctg ccgccgctga ccgaagacga attggtagaa gtttgggcat tgcgttcgct
     7021 cctgcccaac caccagcaac aactcgacag caaccgtcct ttccgcagtc cgcatcacag
     7081 cgccagcgcg gcggctatgg tcggcggcgg ttccgacccg cgtccgggcg agatttcatt
     7141 ggcgcaccac ggcgtattgt ttttggacga gctgcccgag ttcgaccgca aagttttgga
     7201 agttttgcgc gaaccgttgg aaaacggcga aatccacatt tcccgcgcgg cgcgccaagc
     7261 cgtctatcct gccaaattcc aacttgttgc cgccatgaac ccctgcccgt gcggttatct
     7321 cgggcatccc gtcaaaccct gccgctgcac gcccgaaagc gtcgcgcgtt accgcagcaa
     7381 gatttccggg ccgctgctcg accgcatcga tttgaccatc gaagtcccca gcctgtccgc
     7441 cgccgaactg atgcagcagg aagcgggcga aagcagcgcg tccgttttgg aacgcgttat
     7501 cgccgcccgc gacaaacaat acgcacggca gggcaaagtc aacgccgctt tgagcgtcag
     7561 cgaactcgat tcccaagcct gcatccaaaa agaagcgcag gaagcattgg gcggcctgct
     7621 ggaaaaactc tccctttccg cccgcagctt ccaccgcatc atgcgcgtgg cgcgtacatt
     7681 ggcggatttg gctggcgacg aagaagtcgg cagaagccac gtcatgaaag ccataggttt
     7741 ccgtcgtgct ttataggaat gggaatggaa gcaggttttg cccaaatatg gcgatattgt
     7801 tagaatatcc gcccgtaagc aaacggcgtt aatgccgtct gaaacacatt aaggtatgtt
     7861 tatgaacaaa ttttcccaat ccggaaaagg tctgtccggt tttttcttcg gtttgatact
     7921 ggcgacggtc attattgccg gtattttgtt ttatctgaac cagagcggtc aaaatgcgtt
     7981 caaaatcccg gcttcgtcga agcagcctgc agaaacggaa atcctgaaac cgaaaaacca
     8041 gcctaaggaa gacatccaac ctgaaccggc cgatcaaaac gccttgtccg aaccggatgc
     8101 tgcgacagag gcagagcagt cggatgcgga aaaagctgcc gataaagccg acgaggttga
     8161 agaaaaggcg ggcgagccgg agcgggaaaa gtcggacgga caggcagtgc gtaagaaagt
     8221 gctgacggaa gagcgtgaac aaaccgtcag agaaaaagcg cagaagaaag atgccgaaac
     8281 ggttaaaaaa caagcggtaa aaccgtctaa agaaacagag aaaaaagctc caaaagaaga
     8341 gaaaaaggcg gaaaaggaaa aagttgcacc caaaccgacc ccggaacaaa tcctcaacag
     8401 cggcagcatc gaaaaagcgc gcagtgccgc cgccaaagaa gtgcagaaaa tgaaaacgtc
     8461 cgacaaggcg gaagcaacgc attatctgca aatgggcgcg tatgccgacc gccggagcgc
     8521 ggaagggcag cgtgccaaac tggcaatctt gggcatatct tccaaggtgg tcggctatca
     8581 ggcgggacat aaaacgcttt accgggtgca aagcggcaat atgtctgccg atgcggtgaa
     8641 aaaaatgcag gacgagttga aaaaacatga agtcgccagc ctgatccgtt ctatcgaaag
     8701 taaataatta tgaagctcaa acatctgttg ccgctgctgc tgtcggcagt gttgtccgcg
     8761 caggcatatg ccctgacgga aggggaagac tatcttgtgt tggataaacc cattcctcaa
     8821 aaacagtcgg gcaaaattga ggttttggaa tttttcggct atttctgcgt acattgccat
     8881 catttcgatc ctttgttatt gaaattgggc aaggcattgc cgtctgatgc ctatttaagg
     8941 acggagcacg tggtctggca gcctgaaatg ctcggtctgg caagaatggc tgctgcggtc
     9001 aagctgtcag gtttgaaata tcaggcaaac cctgccgtgt ttaaagcagt ttacgaacaa
     9061 aaaatccgct tggaaaacag gtcggttgcc gaaaaatggg ctttgtctca aaaaggcttt
     9121 gacggcaaaa aactgatgcg cgcctacgac tctcctgcgg cagcggctgc tgcatcaaaa
     9181 atgcagcaat tgacggaaca gtaccgcatc gacagtacgc cgaccgttgt cgtcggcgga
     9241 aaataccgcg ttatcttcaa taatggcttt gacggcggtg ttcatacgat taaagaattg
     9301 gttgccaaag tcagggaaga acgcaagcgt cagacccctg ctgtacagaa atagccgaac
     9361 tcccgtatcc gaaagaagcg caagcaatgg attttctgat tgtcctgaaa gccctgatga
     9421 tgggcttggt agaaggtttt accgaatttt taccgatttc cagcaccgga catttgattg
     9481 tgttcggcaa tctgattgat tttcacagca atcacaaggt ttttgaaatt accatccagc
     9541 tcggtgcggt tttggcggta gtgtttgaat accggcagcg tttcagcaat gtgttgcatg
     9601 gcgtgggaaa agaccggaaa gccaaccgtt tcgtccttaa tcttgccatt gcttttatac
     9661 ctgccgccgt gatggggctg ttgttcggca aacaaatcaa agagtatctg tttaacccct
     9721 tgagtgttgc agtcatgctg gttttgggcg gtttttttat tttgtgggtg gagaaacgcc
     9781 aaagccgggc agagcctaaa attgtcgatg ttgatgcatt gcgtccgatt gatgcgttga
     9841 tgatcggcgt tgcccaagtg tttgcactgg ttccgggtac gtcccgttcg ggcagtacga
     9901 ttatgggcgg gatgctttgg ggaatcgagc ggaaaacggc aacggagttt tcatttttct
     9961 tggccgttcc gatgatggtt gcagcaacgg cttatgatgt cctgaagcat taccggtttt
    10021 tcaccctgca tgatgtcggt ttgattctga ttggctttgt tgccgctttt gtttccggtt
    10081 tggtggcggt aaaagcgttg ctgaggtttg tttccaagaa aaattatatt ccttttgcct
    10141 attaccgcat tgtttttggc attgccatca ttatattgtg gctgtcaggc tggataagtt
    10201 gggaatgaaa ccataaaccc gacctgaaga cattattcgg gtcgggtttg tttggcgggc
    10261 tgatatagtg aattaacaaa aatcaggaca aggcgacgaa gccgcagata gtacggcaag
    10321 gcgagccaac gctgtaccgg tttaaattta attcactata aaatcaggac aggcggggcg
    10381 ataggtttaa agtcgattgc ctgttttgaa ggcagtggtt tattctttat ttgctggcaa
    10441 tcaggcaata aaaaagcaca taccttttta cggtctgtgc ttttttatct ggtggaggta
    10501 agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc gctctaccaa ctgagctata
    10561 cccccgaaaa tttggtggcg aatcagggac tcgaaccccg gacacaagga ttatgattcc
    10621 tctgctctaa ccgactgagc taattcgccg tttcgtgaag acgctattat atgtttttct
    10681 gtttttttga caagccgtat tttttaattt tgaattagtt gactgttttt aaatgttaaa
    10741 aagtttatgt cgtctgaagc ggattcaggc ggcatgaggg ttagagtttg tggcagatgt
    10801 cgccgaagcg gaatcctgcc cagtcgacgc cgatattttt accgaatgcg atgactttaa
    10861 acaattctcc catttcatgc tggtcaatca gtttctgaac ggcagcagct tcacggatgt
    10921 aggcgggcga gtctgttttt cccacttgtg ccaacagttc ggttatgccc agattcagca
    10981 gaaaatggga ctggggcaga tagcctgtca aatccagtcc tgcatccgta ccggcttggg
    11041 caatgtcggt aaagttgaca tgcgcggtca ggtcggccaa cccgatgaag tcaaaaggat
    11101 tgtggataac gtgatgtcgg tagtgtccga tcagagtacc ttgattgcgt tgagggtggt
    11161 aatactgcgc tgcatcaaaa ccgtagtcga tgaatatcat acccccgcgt tcaagccttg
    11221 aggcaagggt gcggataaag gcatattgtt gcggatgtag ttcgctggtg tagggataat
    11281 ctgtttgagg aaaatagagg gaagccaagg cagatagctg taagtcgtgc agcggtcgtg
    11341 ccgaataggt aaaacggtca ttatctaggc aaacgccgac atgctcgaat gagccgcctt
    11401 catttttacg gacgatttcg acaggcatgg catcgagtac ttcgttgccg atgatgatgc
    11461 cgtcaaacgc ttcgggaagt gcggtcaagt ggacaacttt ttgagatgct tccggtgcgc
    11521 gtgcttgaat caggtttttc tgacgtgctg ccagctccgg cgatatttca ataatatagt
    11581 aacggctgat gccgtccgaa atgctgccca acaaatcggc ggcaagctgt ccggttcccg
    11641 cgccgaattc atagatattg cccgccgttt gggatagaag ttcttgaagt tggcgtgcca
    11701 gtgtctgtgc aaacagagag gtgagggtcg gtgcggtaat aaaatccccg gtattgccga
    11761 ttttatggct gccgccggtg tagtagccgt attgcggagc gtataaaacc aattccataa
    11821 aacgtgaaaa tggaatccag ttgccgtgtt tgccgatttc ttcggcaatg agggtttgca
    11881 gtttgagcga gaattgccgt gcttcgggag aggggagggg gaggggcatg ataagtgtta
    11941 gcttgtgtaa atttattgga tttcccgaca tattacacgt tggtacgggt gctgtcatgg
    12001 ctttatctta atactatata ttgtgtttat attattaaat taatcatata tagttgttta
    12061 ttggttcgat tattctgtac cgcacccgcc gtgccgttgt cgtcattttt tatcttattg
    12121 tttttaaaag gaataaaaat ttcagatatg ttaatgagtt ttcatgccct gatttgaccg
    12181 agtgtttaaa atttcttata gtgtcgattg gtggggaatt gtggggcaaa gtgtctcttt
    12241 tacccttgtg attttgattt cggcttggga catgtcatgt tcggcggcgc acacgaatta
    12301 agcatcgaca gtaaggggcg gttggctgtt cctgccaaat tccgtgacat tctgtcgcgc
    12361 ctctatacgc ctgccgtagt ggtaacgctc gagtcgaaac acaagctgtt gatgtaccct
    12421 gttgcggagt gggaaaaggt tgcggcgcaa cttttaaact taaaagtggc ggataaccct
    12481 gttttgcggc ggtttcaaaa tcttttgctg cataacgcgg aaattttgga atgggacagc
    12541 gccggccggg tgctgcttcc tgccggactg aggaaaaggg tggatttcga ccgtgaagtc
    12601 gttttggtcg gtcgtgccaa ccgtttggag ctttggggtc gcgagcagtg ggaggctgag
    12661 atggttcagg ctttggatga cgatcctgac gaacttgcct tccagttggg tcagacggat
    12721 ttgcaattgt gagtggagca gaaagttacc ggcatatcac ggtcttgctg aatgaggcgg
    12781 tggatgcgct tgccgtgcgc gaagacggtg tctatgtgga cggtacgttc ggcaggggag
    12841 ggcattcccg gctgattttg tcgcgtttgg gcgatgcggg gcggttgatt gttttcgaca
    12901 aagaccctca ggcgattgct gtggcagaag agctggcgcg ttcggacaaa cgggtcggtg
    12961 tcgtgcatgg cggttttgct tcgtttcagg cggcattgga cggtttgggt atcggcaagg
    13021 tggacggtgc gctgtttgat ttggggattt cgtctccgca aatcgatgac ggcagccgcg
    13081 gtttcagctt ccgtttcgat gcccctttgg atatgcgtat ggatacgacg cgcggtatgt
    13141 ctgccgcaga gtggatagcg gttgcgtcgg aacaggattt gcacgaggta atcaagaatt
    13201 atggtgaaga gcggtttagc cgccagattg cgcgcgccat tgttgcgcaa cgggcggaaa
    13261 gtccaatcga tacaacccgc aagctggcgc agatcgtggc acaaaacgtc cgtactcgcg
    13321 agcgggggca ggatcctgcg acgcgcacct tccaggcggt ccgcatcttt attaaccgcg
    13381 agcttgaaga agtaggggca gtattgccgc aggtcatgtg tcgtctgaaa gagggcggac
    13441 gtttggcggt cattgctttc cattcgttgg aagaccgcat tgtgaagcag tttgtcaaaa
    13501 aatattcgca acacgcgccc ctgccgcgct gggcggcggt cagggaagcg gatttgcccg
    13561 agctgcccct gaaaatcgtg ggcagggcat taaagccggg tgaggcggaa attgccgcca
    13621 atccgagggc gagaagtgcg gttttgcgtg tggcggagcg gactgccggt ccgataccgg
    13681 aacaatcaca gagaaaaacg tctgaatggc aatgaacaaa ttgaatttcc ttctgctgct
    13741 tgcggtgtgc gtttccgctt tttccgttgt gatgcagcaa aaccagtaca ggctcaactt
    13801 cacagctttg gataaggcga aaaaacagga aatcgccttg gagcaggatt atgcgcaaat
    13861 gaggctgcaa caggcgcgtt tggcgaacca cgaagcgatc agggcggcgg cagaaaaaca
    13921 aaacctccat ccgccggttt cgggcaatac ctttatggtg gagcatcaaa gatagaagca
    13981 gcctgtgtgc cggaatcgga ttcctgcgtc aggataataa taacgagaag taaaaatgtt
    14041 gattaagagc gaatataagc ctcggatgct gcccaaagaa gagcaggtca aaaagccgat
    14101 gaccagtaac ggacggatca gcttcgtcct gatggcaata gcggtcttgt ttgccggtct
    14161 gattgcccgc ggactgtatc tgcagacggt aacgtataac tttttgaaag aacagggcga
    14221 caaccggatt gtgcggactc aaacattgcc ggctacacgc ggtacggttt cggaccggaa
    14281 cggtgcggtt ttggcgttga gtgcgccgac ggagtccctg tttgccgtgc ctaaagagat
    14341 gaaggaaatg ccgtctgccg cacaattgga acgcctgtcc gagcttgtcg atgtgccggt
    14401 tgatgttttg aggaacaagc tcgaacagaa aggcaagtcg tttatctgga ttaagcggca
    14461 gctcgatccc aaggttgccg aagaggtcaa agccttgggt ttggaaaact ttgtatttga
    14521 aaaagaatta aaacgccatt acccgatggg caacctgttt gcacacgtca tcggatttac
    14581 cgatattgac ggcaaaggtc aggaaggttt ggaactttcg cttgaagaca gcctgcatgg
    14641 cgaagacggc gcggaagtcg ttttgcggga ccggcagggc aatattgtgg acagcttgga
    14701 ctccccgcgc aataaagccc cgaaaaacgg caaagacatc atcctttccc tcgatcagag
    14761 gattcagacc ttggcctatg aagagttgaa caaggcggtc gaataccatc aggcaaaagc
    14821 cggaacggtg gtggttttgg atgcccgcac gggggaaatc ctcgccttgg ctaacacgcc
    14881 cgcctacgat cccaacaggc ccggccgggc agacagcgaa cagcggcgca accgtgccgt
    14941 aaccgatatg atcgaacccg gttcggcaat caaaccgttt gtgattgcga aagcattgga
    15001 tgcgggcaaa accgatttga acgaacggct gaatacgcag ccttataaaa tcggaccgtc
    15061 tcccgtgcgc gatacccatg tttacccctc tttggatgtg cgcggcatca tgcagaaatc
    15121 gtccaacgtc ggcacaagca aactgtctgc gcgtttcggc gccgaagaaa tgtatgactt
    15181 ctatcatgag ttgggcatcg gtgtgcgtat gcactcgggc tttccgggcg aaactgcagg
    15241 tttgttgaga aattggcgca ggtggcggcc tatcgaacag gcgacgatgt ctttcggtta
    15301 cggcctgcaa ttgagcctgc tgcaattggc gcgcgcctat accgcactga cgcacgacgg
    15361 cgttttgctg ccggtcagct ttgaaaaaca ggcggttgcg ccgcaaggca aacgcatatt
    15421 caaagaatcg accgcgcgcg aggtacgcaa tctgatggtt tccgtgaccg agccgggcgg
    15481 caccggtacg gcgggtgcgg tggacggttt cgatgtcggc gcgaaaaccg gcacggcgcg
    15541 caagttcgtc aacgggcgtt atgccgacaa caaacacatc gctaccttta tcggttttgc
    15601 ccccgccaaa aacccccgtg tgattgtggc ggtaaccatt gacgaaccga ctgcccacgg
    15661 ttattacggc ggcgtagtgg cagggccgcc cttcaaaaaa attatgggcg gcagcctgaa
    15721 catcttgggc atttccccga ccaagccact gaccgccgca gccgtcaaaa caccgtctta
    15781 atccgagtat caacgagatt gttttatgtt cagcaagtta acccctttgg ctgaaaccgg
    15841 catcccgacc ctgtcgtgtg caaacgcagc agggcgtttg ttgcattcag acagccgcca
    15901 aatcaaacaa ggcgatattt tcgttgcctg tccgggcgaa tatgccgacg gccgcagtta
    15961 tatccccgcc gccgttgcca acggcgcggc ttttgttttt tgggacgacg acggcaaatt
    16021 cgcgtggaat cccgaatgga aagtccccaa tcaaggcatc aaagatttga aacaccgtgc
    16081 cggcatattg gcggcgcaag tttacggcaa cgtttcagac ggtctcaaat tctggggcgt
    16141 aaccggaacc aacggcaaaa cctccatcac gcaatggctg gcgcaagctg ccgatttgtt
    16201 gggcgaaaaa accgccattg tcggcacggt cggcaacggc ttttggggtg cattggaaga
    16261 aaccacgcat accacacccg cccccgtcga tgtccaaacc ctgctctacc gtttccgtca
    16321 acaaggcgca acagccgccg cgatggaagt ctccagccac ggtcttgacc agtcgcgcgt
    16381 caacggcgtg ccattccgca gcgcaatctt taccaatctc acccgagacc acctcgacta
    16441 ccacggcacg atggaagcct atggcgccat caagtcgcgc ctgttttact ggcatggctt
    16501 gaaacacgcc gttatcaatg tagatgacgg atacggcgca gaactcatag gccgtctgaa
    16561 aaaagactgc cccgatttgg ccgtttatag ctacggcttc agcgaacacg ccgacatccg
    16621 cattaccgac tttaccgcct cttcagacgg catagaagcc gtattccgaa ccccgtgggg
    16681 tgaagggaaa tgccgcacac gcctgctcgg acggttcaac gcgcaaaacc tcgccgcctg
    16741 catcgccttg ctgtgcgcca acgactatcc gttggataag gtattggatg tgctggcaaa
    16801 aatccgtccc gcttcagggc gcatggactg catcatgaac agcggcaagc ccttggtcgt
    16861 cgtcgattac gcccacacgc ccgacgcatt ggaaaaagca ctcgccacct tgcaggaaat
    16921 caaaccgcag ggtgcggctt tatggtgcgt attcggttgc ggcggcaacc gcgatcgcgg
    16981 caaacgcccg ctgatgggcg cggcagccgt acagggcgcg gataaagtcg tcgtcaccag
    17041 cgacaacccg cgtttggaaa atccgcacga catcatcaac gacatcctgc ctgccgttcc
    17101 cgcgcccgaa tgcgtcgaag ccgaccgtgc cgccgccgtc cgttatgcgg ttgaacaagc
    17161 cgccgcaaac gacatcatcc tgattgccgg caaagggcat gaaaactatc aggatgtaca
    17221 aggcgtgaag caccgttttt ccgatcttga aatcgtcgga caggctttgt taactcgtaa
    17281 ataatgggat attcggacgg catcgtatga aacaatccgc ccgaataaaa aatatgaatc
    17341 agacattaaa aaatacattg ggcatttgcg cgcttttagc cttttgtttt ggcgcggcca
    17401 tcgcatcagg ttatcacttg gaatatgaat acggctaccg ttattctgcc gtgggtgctt
    17461 tggcttcggt tgtattttta ttattattgg cacgcggttt cccgcgcgtt tcttcagttg
    17521 ttttactgat ttacgtcggc acaaccgccc tatatttgcc ggtcggctgg ctgtatggtg
    17581 cgccgtctta tcagatagtc ggttcgatat tggaaagcaa tcctgccgag gcgcgtgaat
    17641 ttgtcggcaa tcttcccggg tcgctttatt ttgtgcaggc attatttttc atttttggct
    17701 tgacagtttg gagatattgt gtatcggggg gggtatttgc tgacgtaaaa aactataaac
    17761 gccgcagcaa aatatggctg actatattat tgactttgat tttgtcctgc gcggtgatgg
    17821 ataaaatcgc cagcgataaa gatttgcgag aacctgatgc cggcctgttg ttgaatattt
    17881 tcgacctgta ttacgatttg gcttccgcgc cggcacaata tgccgccaag cgtgcccaca
    17941 ttttggaagc agcaaaaaaa gcgtcaacat ggcatatccg tcatgttgcg cccaagtata
    18001 aaaattatgt tgtggttatc ggtgagagcg cgcgttcgga ttatatgaat gtttacggtt
    18061 tcccattgcc cgatacgcct tttttgagtc agaccaaagg gctgttgata aacggttacc
    18121 aatcgaccgc ccacgcgacg aatctttcgc tgccgcagac tttggggctg ccgggagaac
    18181 cgaacaataa catcgtcagc ttggcgaagc aggcgggttt tcggacggcg tggctgtcta
    18241 atcaaggaat gttggggcat tttgccaacg aaatttccac ctatgcccta cgcagcgatt
    18301 atccgtggtt tacccaaagg ggtgattatg gcaaaagcgc ggggttgagc gaccgccttt
    18361 tgttgccggc gttcaaacgg gttttgatag gaaatgcagg cacgaagcct cggctgattg
    18421 tgatgcacct gatgggttcg cacagtgatt tttgcacacg tttggataag gatgcgcggc
    18481 ggtttcagta tcaaactgaa aaaatatcct gctatgtttc caccatcgcg caaaccgata
    18541 aatttttaga agatacagtt aagatattga atgaaaataa agaaagctgg tctttggttt
    18601 acttttccga ccacggtttg atgcatgtcg gtaaaggcgg cgagcgaacg ttgacacatg
    18661 gtgcgtggaa gcgtcaaagc tacggcgtgc cgctggttaa aatttcgtcc gatgacacgc
    18721 ggcgcgaaat gattaaagtg aggcgcagcg cgtttaattt tttacgcgga ttcggcagtt
    18781 ggacgggtat cgaaaccgac gagttgcccg atgacggcta tgatttttgg gggaatgttc
    18841 ccgatgtgca gggcgaaggc aataaccttg cctttatcga cggactgccc gacgaccccg
    18901 cgccgtggta tgcgggaaaa ggcaaatcga ctaaaaatac gtctaaaaaa tgatacgtac
    18961 agaaaaaatg ccgaatgaga atgggaaaat aatctgtgtt ttaccacagc aaaacaggcg
    19021 ataaaaaaaa tcagccgcta ccgatgtgtc cgccgcccga atattaacga aagtaaatat
    19081 gaaaccactg gacctaaatt tcatctgcca agccctcaag cttccgatgc cgtctgaaag
    19141 caaacccgtg tcgcgcatcg taaccgacag ccgcgacatc cgcgcgggcg atgtgttttt
    19201 cgcattggcg ggcgggcggt ttgatgcgca tgattttgtt gaagacgtat tggctgctgg
    19261 tgcggcggcg gttgtggttt cgcgcgaaga tcgcgttgca atggatggcg cgttgaaagt
    19321 cgatgacacg cttgccgcat tgcaaacgct ggcaaaggcg tggcgcgaga atgtgaaccc
    19381 gtttgtgttc ggtattaccg gctcgggcgg taagacgacg gtgaaggaaa tgttggctgc
    19441 ggtattgcgc cgccgtttcg gcgataatgc cgttttggcg acggcaggca acttcaacaa
    19501 ccacatcgga ttgccgttga ctttgttgaa attaaacgaa aaacaccgct atgccgtgat
    19561 tgaaatgggt atgaatcatt ttggcgaact ggcggttttg acgcaaatcg ccaaacctga
    19621 tgccgccttg gttaacaacg ccctgcgtgc ccatgtcggc tgcggtttcg acggagtggg
    19681 cgatattgcc aaagcgaaaa gcgagattta tcaaggctta tgttcagacg gcatggcact
    19741 gattcctcaa gaagatgcca atatggctgt cttcaaaacg gcaacgctta atttgaatac
    19801 gcgcactttc ggcgtcgata gcggcgatgt ccacgcggaa aatatcgtgc tgaaaccgtt
    19861 gtcgtgcgaa tttgatttgg tgtgcggcga tgagcgcgcc gccgtggtgc tgcctgttcc
    19921 cggccgccac aatgtccaca acgccgccgc tgccgccgcg ctggctttgg ctgcgggttt
    19981 gagtttgaac gatgtggcgg aaggtttgaa aggcttcagc aatatcaaag gccgtctgaa
    20041 cgtcaaatcc ggaatcaagg gcgcaaccct gattgacgat acttataatg cgaaccctga
    20101 cagcatgaaa gctgcgattg acgtgttggc gcgtatgcct gcgccgcgta ttttcgtgat
    20161 gggcgatatg ggcgaactgg gtgaggacga agccgccgcc atgcacgccg aagtcggcgc
    20221 gtatgctcgc gaccaaggca tcgaagcggc ttattttgtc ggcgacaaca gcgtcgaagc
    20281 ggcggaaaaa tttggcgcgg acggtttgtg gttcgccgcc aaagacccgt tgattcaagt
    20341 gttgcgccac gatttgcccg aacgcgccac cgtgttggtg aaaggttcgc gctttatgca
    20401 gatggaagaa gtggtcgagg cattggagga taagtgaaaa tgaaaagtcg acgttttttt
    20461 aaagccttat tgttgattgc cgcgctggtc ggcgcgtttt atgccggaat gcagacgcag
    20521 gcgtatcttt atgaagattt atgtttagac ttgggtggcg gcaaaaatcc ggggagttac
    20581 ccgatttgcg tgattgagaa agtccctgca cgttaatctg caaaagccgt ccgaaacctt
    20641 gccgggcggc aagccaacct caaacgggcg caggcccga
//
LOCUS       DAWEWP010000020        20614 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWEWP010000020 DAWEWP010000000
VERSION     DAWEWP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20614
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.020"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..231)
                     /locus_tag="WGN53_000530"
     CDS             complement(<1..231)
                     /locus_tag="WGN53_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide succinyltransferase"
                     /protein_id="HEZ0139554.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARID"
     gene            complement(331..3159)
                     /locus_tag="WGN53_000531"
     CDS             complement(331..3159)
                     /locus_tag="WGN53_000531"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ0139555.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVALCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(3364..4647)
                     /gene="gltA"
                     /locus_tag="WGN53_000532"
     CDS             complement(3364..4647)
                     /gene="gltA"
                     /locus_tag="WGN53_000532"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0139556.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(4759..5007)
                     /locus_tag="WGN53_000533"
     CDS             complement(4759..5007)
                     /locus_tag="WGN53_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ0139557.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(5011..5718)
                     /locus_tag="WGN53_000534"
     CDS             complement(5011..5718)
                     /locus_tag="WGN53_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ0139558.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            complement(5839..7602)
                     /gene="sdhA"
                     /locus_tag="WGN53_000535"
     CDS             complement(5839..7602)
                     /gene="sdhA"
                     /locus_tag="WGN53_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ0139559.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPTKRVY"
     gene            complement(7605..7946)
                     /gene="sdhD"
                     /locus_tag="WGN53_000536"
     CDS             complement(7605..7946)
                     /gene="sdhD"
                     /locus_tag="WGN53_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ0139560.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(7940..8317)
                     /gene="sdhC"
                     /locus_tag="WGN53_000537"
     CDS             complement(7940..8317)
                     /gene="sdhC"
                     /locus_tag="WGN53_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ0139561.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(8596..9999)
                     /locus_tag="WGN53_000538"
     CDS             complement(8596..9999)
                     /locus_tag="WGN53_000538"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0139562.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(10248..10985)
                     /locus_tag="WGN53_000539"
     CDS             complement(10248..10985)
                     /locus_tag="WGN53_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ0139563.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(11088..11252)
                     /locus_tag="WGN53_000540"
     CDS             complement(11088..11252)
                     /locus_tag="WGN53_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139564.1"
                     /translation="MNKYRLKAFQTVFCPDLRRKGAVAGKSFHCSLFFSNSALYVGNR
                     QIGVVFDSFK"
     gene            complement(11264..13540)
                     /gene="metE"
                     /locus_tag="WGN53_000541"
     CDS             complement(11264..13540)
                     /gene="metE"
                     /locus_tag="WGN53_000541"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ0139565.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGTVEFNRLSLLQKLLPVYVEILNALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGIIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(13678..14556)
                     /gene="metF"
                     /locus_tag="WGN53_000542"
     CDS             complement(13678..14556)
                     /gene="metF"
                     /locus_tag="WGN53_000542"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ0139566.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDELPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            14756..15031
                     /locus_tag="WGN53_000543"
     CDS             14756..15031
                     /locus_tag="WGN53_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ0139567.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            15031..15156
                     /gene="ykgO"
                     /locus_tag="WGN53_000544"
     CDS             15031..15156
                     /gene="ykgO"
                     /locus_tag="WGN53_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ0139568.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(15262..15462)
                     /locus_tag="WGN53_000545"
     CDS             complement(15262..15462)
                     /locus_tag="WGN53_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139569.1"
                     /translation="MIGRLFRIFFFFALAALIMNRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            15605..16744
                     /locus_tag="WGN53_000546"
     CDS             15605..16744
                     /locus_tag="WGN53_000546"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ0139570.1"
                     /translation="MSQNASVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHTLVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            16741..17322
                     /gene="metW"
                     /locus_tag="WGN53_000547"
     CDS             16741..17322
                     /gene="metW"
                     /locus_tag="WGN53_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ0139571.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            17439..18284
                     /locus_tag="WGN53_000548"
     CDS             17439..18284
                     /locus_tag="WGN53_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ0139572.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(18334..18894)
                     /gene="efp"
                     /locus_tag="WGN53_000549"
     CDS             complement(18334..18894)
                     /gene="efp"
                     /locus_tag="WGN53_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ0139573.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(18937..20085)
                     /gene="earP"
                     /locus_tag="WGN53_000550"
     CDS             complement(18937..20085)
                     /gene="earP"
                     /locus_tag="WGN53_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ0139574.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTALVPDIVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSLQESVQKYFWFMGFSEKSGGLIRERDYRNA
                     VRFDTEALRERLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(20104..20382)
                     /locus_tag="WGN53_000551"
     CDS             complement(20104..20382)
                     /locus_tag="WGN53_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139575.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKLQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
BASE COUNT         5121 a         5224 c         5169 g         5100 t
ORIGIN      
        1 atcgatacgt gccaaaactt ggtcggcaac aacggtttca ccgtcttgcg ctacgatttc
       61 aaccaatacg ccggcttgtg gagaaggtac ttccaaaacc actttgtccg tttcgatatc
      121 gatcaggatt tcgtcacggg caactgcttc gccaactttt ttcttccatt ccaagagcgt
      181 gccttcagat acgctttcag acagcatagg tacttttaca tcaataatca ttttgtgtct
      241 ccaatggccc tttcagacgg cctgttgtgt gtttttcatt ctgcatatgg gtgttccgcc
      301 tgagcagcct cagacggaat gctacttggc ttacaatgcc aaagcgtctt caaccaattg
      361 tttcaattga gcaatgtgtt tgcttgagta gcccactgca ggcgatgcgc tgcttggacg
      421 accggcataa gacagttttt gctcttcgct gataacgtct tcgatgcggt ggcggatttg
      481 gtagaacgcg ccttggtttt tcggctcttc ttgtgcccaa accacagatt ttgcgttcgg
      541 atatttcgcc agttcggctt taacctcgtc gtatgggaac ggatacagct gctcaacgcg
      601 gacaatagcg acatcatctt ccagtttacg ttcggctcga ccggcttcca agtcatagta
      661 aacctgaccg gcacacaatg ccacgcgttt cacgctgtcg ttgcttgcgc gttcggcggt
      721 atcgccgata accggacgga aagtcgaacc ttcggtgaag ttttccagcg ggctcattgc
      781 ccctttgaat cgcaacaggc gtttggacat gaaaatcact agcggtttgc ggtatgaacc
      841 taagacttga cgttgcaaga ggtggaacat ttgcgacgct tcagacggca tgatgacttg
      901 catattgttc tcagaacaca gttgcaacca acgttctacg cgtgcagaag agtgctcggg
      961 accttgaccg tcgtagccgt gcggaaggat ggtggtcaga ccgcacaaac gaccccattt
     1021 ggtttcgcct gaagacagga attggtcaat agtcacttgc gcgccgttgg cgaagtcacc
     1081 aaattgagct tcccaaatgg tcagcttgtc aggcgcggag caggcaaagc cgtactcgaa
     1141 cgccatcacg gcttcttcgt tcaaaatgga gtcgataacc aagaactcgc ccataccttc
     1201 gcccatatgg cgcagaggaa cataagtacc gtcatcccat ttttcacgtt tttgatcgtg
     1261 caatactgca tgacggtgtg agaatgtacc gcgcccggag tcctcaccgg agatacgcac
     1321 gccatgacct ttggttacga ggctggcgta tgccaaggtt tcggccatac cccaatctat
     1381 ggcctgtttg ccggatgcca tggctttacg cgcttcaatc acacgttttg cagtcggatg
     1441 cagggcaaag ccttccggta cggcggtaaa cttctcagtg agacgctcaa tatctgcggc
     1501 aggcaaaccg gtttcgatgt gttcgcgcca atctttgcct tggtatttgc tccagtcgat
     1561 ttgcgtgcgt tgaaagttgc tcaacgttgt ttgctcgacg tgttcgcctt tgtccaaagc
     1621 atcacggtag gcttggatgt aaccgtctgc ctcggcttgg gttaccacgc cttcggcaat
     1681 cagttgctcg gtgtacaaag cacgcgcacc ggggtgttgc gatacttttt tgtacatcat
     1741 cggttgggtc aaggtcggat catcgccctc gttgtgaccc catttacggt agcagacaac
     1801 gtcaatcacg atgtctttat ggaatttttt gcggtaatcc aaagcggctt ggatggcaaa
     1861 gcaaacacgc tcaggatcat cgccgttcac atggataacc ggagcggaaa ccatttttgc
     1921 gatatcggta cagtgtacgg ttgaacgggt atcgcggata tcggaagtgg taaagccgat
     1981 ttgattgttg atgacgatat gaaccgtacc gccggtggta taaccgcgcg ttttagacag
     2041 gttgaatgtc gcttggttga cacccaaacc gataaatgcg gagtcgccgt gaatcaatac
     2101 gggcaagact ttatcgcggc cgttttcgcc caaacgtttt tgcttggcgc gtgcagaacc
     2161 ttccactacc ggattgacga tttccaagtg tgacgggttg aacgccaaag aaacgtgcat
     2221 cggaccatgt ggagtggcga tgtcggagct gaagcccatg tggtatttca cgtcgccgct
     2281 aggcaatttg atttcggcac ggccttcaaa ctcagcgaac aaatctccgg gttttttacc
     2341 caaaatgttc accaaaacat tcagacggcc acggtgcgcc ataccgatga tgacttcttc
     2401 cacgccgtct ttaccggcgt tttgaatcag gtagttcaaa ccggcaatcg cgctttcgcc
     2461 gccttcgaca ccgaaacgtt tctgaccgac atatttggta tgcagataac gttccaaagt
     2521 ctcggcagca gtcatctctt tcaagatacg gcgtttttga tcggcattgt aatgcggtgt
     2581 ggacaatacg ctttcaaaat aattgcgtac ccagcggcgc tcttcggtat tgggaatata
     2641 gatatattcc aatgcgatgt ggccgcagta ggtttgtttg aggttgctga tgatttggga
     2701 caaaggcagt ttgccgcgat tggcaaaatc gccctcgccc atattgaatt gaagcgccat
     2761 atcggcatca gacagaccgt ggaatttcgg atcgagggct tcaatatcgc gcggggggat
     2821 acgtttgagc ggatcaagtt gggctgcacc cacgccttgg atacgatagg cagaaatcag
     2881 ccgtaaaacg ctgacttgct tcttcagcat tgcctcatcc gcaccgcccg caacggcaga
     2941 tgcaattttc tttttcgcca aagtaacaaa tgattcgcga atcggtgtgt gtgcgacatc
     3001 gacagcaacc gtccccggct gtttgctcaa atcggtgaaa tactgcttcc atttttcatc
     3061 aaccgcatcg gggttttcca aaaaagcctc gtacaattcc tcaatgtagg gtgcgttcga
     3121 accgaacagg taagagaaat tgagtttttc gtccatcatg gcgtgcgctc ttttctttaa
     3181 aatataaaca aagggtagaa gtccgcgtcc tgacggctga actgatgccg tctgaaaaga
     3241 cattttattc taccctattt taaaccggat tgcctgtatt gatttcatcc gactacaatc
     3301 aaatgtatac agaaagcaat ccgattctct aatattgcct gtttgacaat atattaatgt
     3361 atgttacctc tctcctgccg gcacataatc gcggcgttcc gaaccggtat aaagctggcg
     3421 cgggcggccg attttcagcg aaggatcgct gatcatctcg tgccagtgcg aaatccagcc
     3481 cacgctgcgc gacagggcga agatgacggt aaacatttcg gtcgggatgc ccagcgcgga
     3541 caggacgatg ccggaataga aatcgacgtt tggatacagc ttgcgttcga taaagaacgg
     3601 gtctttcagc gcaatctgtt ccaattccat cgccagtttg aatttcggac tgtcttccaa
     3661 gcccaattcc ttcaaaactt catagcaggt ttcgcgcata atgctggcac gcggatccat
     3721 attgcggtac acgcggtgac cgaagcccat cagacggtat ttgcgttgtt tcacaccttc
     3781 catgtatgcg gcaacattag acacatcgcc gatttcgtcc aacattttca acacggcttc
     3841 gttcgcaccg ccgtgcgaag caccccacag gcaggcgata ccggcagcaa tacaggcaaa
     3901 cgggttcgca cccgaagacc ctgccagacg gacggttgaa gttgaggcgt tttgctcgtg
     3961 gtcggcatgc aaaataaaga tgcggtcgag cgcgcgtgcc aaaacgggat tgggtttgta
     4021 gtcttcacac ggcgtggcga acatcatatg aaggaagttt tcggaataag aaagattatt
     4081 cttcggataa ttgaacggca gaccgtttga atagcggtag cacattgccg caatggtcgg
     4141 gattttggaa atcaggcggt aaatcgcgat tttgcggtgt tcgggattgc taatgtccaa
     4201 gctgtcttgg tagaacgcag acagcgcacc gaccacgccg accatcatcg ccatcggatg
     4261 cgcgtcgcgg cggaaccccc ggaagaacca agtcagctgt tcatgcacca tcgtgtggcg
     4321 gcggacggta ttgtcaaatt ctgccttttg ctcgggagtc ggcagttcgc cgtaaatcaa
     4381 caggtagcag acttccaaat aatcggactt ttcggccagc tgctcgatgg ggtatccgcg
     4441 ataataaagc aagccttgat cgccgtcgat gtaagtaatt ttagactcac agcttgcggt
     4501 tgaaacaaat ccggggtcga aagaaaacaa acctgtattt tttgtcagcc cccgaatgtc
     4561 aaccacatcg tgcccgatgc tggcttccaa taccggcagc tccaaacctg cctgacccgg
     4621 tacgttgagt ttgattgatt tggacatatt attgctcctt tgcaagttat aacccacgtt
     4681 actgcatccg cagcacgttc tttgccttca gacggctttt gcattttgaa atctgcctgc
     4741 aggcagattt cagatgcctc atgcccgtct gattttttca agcatcggga taaggtgccc
     4801 tttgtccgtt tccgaatgcc cgttaatcaa ggcaagcaat tcttgatctt gaaattcaag
     4861 gatttcggaa aactcggaca gctctttatc gctcaaatgc tcgaattctt tttccataaa
     4921 cctgccgaag attaaatcta attccaacaa tccccggcgg gtttgaaaac ggattttccg
     4981 tttggcaata tcgtcaaaaa ccatcatttc ttaaacgacc cgtttcaaca taatctcttt
     5041 aatcttaccg atggctcggg tcggattcaa gtgtttagga catacgtcta cgcagttcat
     5101 aatggtgtgg caacggaaca aacggtatgg gtcgttcaga ttatccaaac gctcattagt
     5161 gatggtatca cggctgtccg caatgaaacg gtaagcattc agcaaaccgg acggaccaac
     5221 gaatttatca gggttccacc aaaatgacgg gcaggcagtc gaacagcagg cgcacaaaat
     5281 acactcgtac aaaccgtcca actctttacg ctcttcctga gtttgcagac gctctttgtc
     5341 cgcatcaatc ggattatcgt tgacaacata aggtttgacg gaatggtatt gtttgaagaa
     5401 ctgggtcata tccacaatca ggtcgcggat aacaggcaga cctggcagag gacggatttt
     5461 aaccggctgt ttcaagccac gcagatcggt caaacacgcc aagccgtttt tgccgttgat
     5521 gttcataccg tccgatccgc aaatgccttc gcggcaggag cggcggaaag acaaggtatc
     5581 gtcttgtgct ttcaggcgta ccaaagcatc caaaagtttc acgtcggtcg gttccaattc
     5641 caactcgtaa cgctgcatat aaggcttggc atcaacatcc gggttgtaac ggtaaatttc
     5701 aaaactcatt ttttccatga gaagtgttcc ttttctcatc aactgcccaa tgattttgta
     5761 ttataaattc aagcagttca atgtgggtat ggttagattt cagacgagct tatgaggcga
     5821 agaccgtctg aaaacgcatc aataaacgcg cttggtcggt ttgatgtatt ccacgctcaa
     5881 aggcttggta tgtaccggtt tgtaagacaa agtattggta tctgaatggt acagcgtatg
     5941 tttcatccag ttttcatcat cgcgctcagg atggtcgtct gaagcgtgcg caccgcgtga
     6001 ttctttacgt gcttcggcag acaccaaagt cgctttcgcc acttcaatta ggttatccaa
     6061 ttccaaagcc tcgatacgcg cggtattcca cactttgctc ttgtctttga tttcggtacg
     6121 tttcacacgc tcggcaatcg ccatgacttc tcgaacgcct ttgctcagaa tctcatcagt
     6181 acggaacacg ccggcgtgca attgtacgga gcgttgcagt tcgcggcgca atgcatcaac
     6241 gttttcacca tcggtttggc tgtccaaacg ctcgatacgt tggcgggtca actcaccagc
     6301 attagcaggc aaaggtttcc agtcgctttg ctctttgatg aatttaatca tgctgtcgcc
     6361 ggcagcttta ccgaatacca ccaagtccaa cagggagttg gtacccaagc ggttcgcacc
     6421 gtgtacggaa gcacaagcgc actcacctgc cgcatacaga ccttttacag gcacttcgta
     6481 ttcgtcgcct tgaggaacga caacttcgcc atggtagttg gtcggaatac cgcccatcat
     6541 atagtgggta gtcggcacaa cgggaatcgg gtctttaatc ggatcgatac cggcgaactg
     6601 aatggaaatc tcgcggatgc ccggcagttt ttccataatt ttttctgcgc cgatatggtc
     6661 gattttcagt aagacatggt ctttgttttt accgcagccg cgaccttcgt agatttccat
     6721 cgccatcgcg cgggaaacaa cgtcgcgaga agccaagtct tttacggtcg gcgcatagcg
     6781 ttccataaag cgttcgccgt cggcattcaa cagaataccg ccctcgccgc gtacgccttc
     6841 ggtaatcaac acgcccgcac ctgccacgcc ggtcgggtgg aattgccaga attccatatc
     6901 ttccaacgga atacccgcac gcgcgcaaat gcccaaacca tcgccggtat tcatataggc
     6961 attggtagaa gaagcataaa tacgaccgcc accgccggta gcaaacatca cagctttagc
     7021 gtggaaaata taaacttcgc cggtttccat ttccatggcg gttacgccga cgacatcgcc
     7081 gttttcatca cgaatcaaat cttgtgccgt ccattccaca aagaattgcg tattggcacg
     7141 gacgttttgt tggtacaaag tatgcagcat cgcatgacct gtacggtcgg caaccgcaca
     7201 ggcgcgttct accgcgcgtt taccgtgttc ggcagtatgg ccgccgaaag gacgctgata
     7261 aattttaccg ctttccacac ggtcaaaagg catacccatg tgttccaact caattacggc
     7321 ttcaggcgcg gcgcggcaca taaactcaat cgcatcttgg tcgcccaacc agtcggaacc
     7381 tttcacggta tcgtacatgt gccagtccca acggtcttcc tgcacattac ccagagaggc
     7441 ggaaataccg ccctgcgccg ctacggtatg cgaacgggtc gggaacactt tagacaaaac
     7501 ggcacaattc agaccggatt tggataattg gagggctgcg cgtaaacctg caccaccacc
     7561 gccgacaatc acggcatcaa acttgcgaac aggaaaaccc atacttaccc ccaaattact
     7621 ttaattgaat acaccaagca gccgaccagc cagacgatgg tggcaacctg caaaaacaaa
     7681 cgcacgccga agggtttgat atagtccatc cacaaatcgc ggatacccac ccaagcgtgc
     7741 aagaatacgg cgatgaagct cacttgggta aatactttta cccaagtttg actaaaaaat
     7801 gcctgccatg ccgaatattc tttaggcagg gaaaatagaa ccactaaaag tgcaacggta
     7861 taaatcaaca taataaccgc agtcgcacgt tgcatcgccc aatcgcgcaa accgtaatgg
     7921 gcaccggtca atttacgttc taccataaca acgctcccaa aacgacagtc aaaaccaatg
     7981 cagaagcaaa tacggcttta gcggtattgc gcgcagtatt cagctcaagg cctttgtgcg
     8041 catccaaaaa taaaaagcgg ataccggcga gagaatggtg cagataagcc cacaatacac
     8101 cgattaaaac cagcttgacc aaaggatggg aaacaatggc acggtaagtt tcaaatgcag
     8161 actcttgact cagggtaccg gacaggaaat acagcaggaa aggcagcata ataaacagcc
     8221 cgaccccgct gatgcggtga aggatggaaa ctatcccggg tatcggcaga cggatgttcg
     8281 gcaaatccag ataaacagga cgcggtttgg cagacatagt caattcctct gtagttatat
     8341 atcgactcaa aaaccgtaaa cccattgcta cactgggtta cataaacgtt aatttacctg
     8401 ttttatccca ttttgaacag tgtattttaa aaacaaactg atagattttg acaaacacgc
     8461 gtggaaacag cagattaaaa gaaagatttt tagttttgcc gcccccttcc cgacacggta
     8521 tcagcatccg accccatccc caaaaacaaa atgccgtctg aaaaaatttc agacggcatt
     8581 gttttgccat atcggtcagg ctttcaattt cgcatcggca tcgcgcaacg cggtacgcag
     8641 accttcctcg ataacgggat ggtagaacgg catatccagc atttgcggaa cggtcatctt
     8701 catttgatgc gcccatgcca gcaagtgcgc caaatgttcg gcggcagggc ctacgatttc
     8761 cgcgccgata aagcggccgg tggctttttc ggcatacagg cgcatatggc ctttgttcac
     8821 cagcatcacg cggctgcggc cttggttttt gaacgatact tcgccgatga caaattcgtc
     8881 ggcttggtat tgcgcggcaa cctgcgcgta tttcagaccg acaaagccga tttgcggact
     8941 ggtaaacacc acgccgatgg tgctgcgccg caaaccgccg ccgatattcg ggtagcggcc
     9001 cgcgttatcg ccggcaatct tgccttggtc ggcagcttca tgcagcagag gcagttggtt
     9061 ggacgcgtcg cccgcaataa agatatgcgg aatgctggtc tgcatggtca gtgggtcggc
     9121 aacgggtacg ccgcgcgcgt ctttttcgat attgatgttt tccaaaccga tattgtcaac
     9181 gttcggacgg cggcccacgg ctgccagcat atattcggca acaaatacgc ctttttcgcc
     9241 gtcctgctcc caatggactt ctacattgcc gtctgcatct agtttgacct cggttttagc
     9301 atccagatgc agtttcaatt cttcgccgaa cacggctttc gcctcgtctg aaacgacggg
     9361 gtcggaaatg ccgccgatga ttccgcccaa accgaaaatt tcaactttca cgcccaaacg
     9421 gtgcaatgcc tgacccagtt ccaaaccgat aacacccggc ccgaacacgg caacgcgctt
     9481 aggcagcgta tcccatgaga aaacgtcatc gttgataatc aaacgattgc ccaaagactg
     9541 ccattgcggc aggatgacgg gacgcgaacc ggtagcaatc acgaaacttt ttgccgtaat
     9601 ttgagtatgc tcgtcgattt ggacggtatg ctcgtcgata aatttagccg aacccatgat
     9661 gcgcttgtcg gcaggccact cttccacatc ggcaacgaca aagccgacaa aacggtcacg
     9721 ctcggattta acgcgctgca tgacctcttc accgttgacg acgatgctgt ctttgtccaa
     9781 atgcacgccg aacgggtcgg tatgcaatgc gtggtggcgc gcttctgcgg cggcaatcaa
     9841 gagtttggaa ggcatacaac ccacgcgcgc gcaggttgtg ccgaacacat tgttttcaat
     9901 caggtaaaca ttatccgaat gtaaacgggc attgcgaaac gcacccatac cggcagtacc
     9961 gccgccgatt acgacgacat ccgcttgaat ttttttcata atctttgtcc ttccgttttc
    10021 gtctgaaaca ttgccgacac agaacatatc ggtttcagac ggcatttaaa tattttccca
    10081 atcaaaaaca tcattcagat aaataaaaac aaaatttcga ttgagtgcaa caatatccaa
    10141 attctcttat aaatatagag ttattcggat cggcagtgta gaaccgcccg aataactcta
    10201 tccggtatat cagacagttt aatcaaaccg ccttaagcaa acagctatca gtttttagcc
    10261 aagtaagctt ccaaatcttc gctgccgccg atgtatttgc cgccgatgaa gacttgaggg
    10321 gcagtcatct tgccggtaat ggcgcgaacg gaagtaacgg ttgcatcttt gcccaatacg
    10381 atttcttcgt aagacaaacc tttgtcttgc aaagcttgtt tggctttggc gcagaattgg
    10441 caacctggtt tagtgaaaat tgccacagac tcttgagctt tccaatcagg agcaacgaat
    10501 ttcagcatag tatctgcatc ggatactttg aacggatcgc ctggttcttc aggctcgatg
    10561 aacatttttt caaccacgcc gtcgttaacc agcatggagt aacgccaaga gcgtttaccg
    10621 aagcccaagt cttctttacc gaccagcata cccatacctt cggtaaattc gccgttgcca
    10681 tcaggaatca tgtagatgtt gtcggattct tcttcggcag cccaagcgtt cattacgaac
    10741 gtatcgttta cagatacgca gtagattgcg tcaacgccgt tttctttgaa cgcgccgaac
    10801 aattcgttgt aacgcggcag gtgtgaagaa gaacaagtcg gggtaaatgc accgggcagg
    10861 gagaatacga ctactttttt gcctttgaac aaatcatcgg tagacacatc tttccaagtg
    10921 tcgccgacgc gggtgcggaa tactacggaa ggtacttttt gaccggtacg atcttgcaaa
    10981 gccattttaa gctccttaat agattggttt gagaaataat tgtgaatcgc tgggcattct
    11041 accccatgca aaatgatttg tataatttat agttcaaata atataaatta tttaaaacta
    11101 tcaaacacaa ctccgatttg cctgtttccc acatataaag ccgaattaga aaaaaacaag
    11161 ctgcaatgaa aagattttcc ggcaactgcg cccttgcgcc gcaaatcagg acaaaatacc
    11221 gtctgaaagg ctttcagacg gtatttattc atacggtctc ggcttatttc gccaattcgg
    11281 cacgcaattt gtgggttacg ttcatcatca cttggagttg ttccagagtt tctttccagc
    11341 cgcgtgtttt caggccgcag tccgggttaa cccacagacg ttcaaccggt acaacctcga
    11401 tggctttgcg caacaggtgc tccacttcgg cttctgtcgg tacgcgcggg ctgtggatgt
    11461 cgtaaacccc cgggccgatg tcgttcgggt atttgaattc gccgaacgcg gtcaagagtt
    11521 ccatgtcgga acgtgaagtc tcgatggtga tcacgtccgc atccattgca gcaatcgcag
    11581 gcaggatatc gttgaactcg gagtagcaca tatgagtgtg gatttgtgtg ctgtcttcgc
    11641 aaccggcaga ggacaggcgg aaggattcgc ccgcccagtt caggtaggca tcccaatcgg
    11701 cgcgtttcag cggcaagcct tcgcggatgg caggttcgtc aatttggatg actttgatgc
    11761 ctgctttttc cagatccaat acttcgtcgt tcagagccag tgcgatttgt ttgcacacgg
    11821 tagagcgagg aatatcgttg cggacgaaag accattgcag aatggttaca gggccggtca
    11881 acataccttt catcggacgt ttggtcaggc tttgagcgta agtagaccaa gcgacagtca
    11941 tggcttcagg acggcttacg tcaccgaaga taatcggtgg tttaacacaa cgtgagccgt
    12001 agctttgtac ccagccgtat tgggtgaatg caaaaccgct caacaattcg ccgaagtatt
    12061 cgaccatgtc gttacgctcg gcttcgccgt gtaccagtac gtccaaatcc agtttttctt
    12121 gttcttcaac caccaaggcg atttcttttt tcatcgcggc ttcgtaatcg gcggcagaca
    12181 gttcgccttt tttgaaggct gcgcgtgctt ggcggatttc ggtagtttgc gggaaagaac
    12241 cgatgttggt cgtcggcagc agaggcaggt tcaaccatgc ttgttgcgct ttgatacggt
    12301 cggcaaatgg agatttgcgt tggtctgcgt tggcaggcaa atcggccagg cgtttggcaa
    12361 cgtctgcacg gtggatttcg ctgctgttgg cgcgtgaatc ggcagcagct tggctggcgg
    12421 ccagttcttc ggcaacggaa tcgcgacctt cgttcaatgc ggctttcaga acgcgcaatt
    12481 cttgggtttt ttgcagggta aatgccaacc aagagtacag gtccggtttg ttggctttca
    12541 gtttttcttc aactgacaag tcaaatggag tgtgcagcag agagcaagag ctggaaatcc
    12601 acaaacggtc gcccagtttg gcttgcagag gctcaacagt ttccaaaact ttgttcaggt
    12661 tggcgcgcca gatgttgcgg ccgtcgatga tgcctgcaga cagaactttg tcgtagtcgg
    12721 caaatgcgtc cagttgctcg ggagcgcgta ccaagtcgat gtgcaggccg tcaacaggca
    12781 gggatttcaa caatgcggcg tgttcggcaa cagaaccgaa gtaagtgctc agcaggattt
    12841 tggcacttac tttgctcaaa gtagcgtaaa cgtctttgta ggcttctacc cattctttag
    12901 gcagatcgac agtcaaagca ggctcgtcga tttgaatcca ttctgcaccg gcttcaacca
    12961 aagcgtttag gatttcaacg taaacaggca gcagtttttg caacaggctc aggcggttga
    13021 attcgactgt gcctttttct ttacccaccc acaggaaagt caacggacct acaacggtcg
    13081 gtttggcttt cagaccgagg gcttgggctt cttgcagttg ttgaacatag tgtttggcat
    13141 tggctttgaa ttcggtatcg gcgtggaatt caggcaccaa gtagtggtag ttggtgtcga
    13201 accatttggt catttcgata gcgaattggt ctttgttgcc gcgcgccagt tggaagaatt
    13261 gttccaaaga caggttttgg ctgtcgaaac cgaagcgggc gggaatcgcg ccggtggcga
    13321 cttgcaggtc gaggatgtgg tcgtagaaag tgaaatcgcc tacggcaacg aaatcggcgt
    13381 tggcagcggc ctggtgtttc cagtttttct cgcgcaagtc tttagcaaca gccagcaatt
    13441 cttgctcgct gatttctttg cgccagtatt tttcttgtgc gaatttcaat tcgcggaagg
    13501 cgccgacacg cgggaagcct gagaaatgta atgttgtcat gttgaactct ccagttggat
    13561 tttgtaagga ccggttcgat ccaaatcggc cggtattgta aatcagaatg gctcggatgg
    13621 ggcggtgttt tgctttttag acaccttaga tgccgtctga aactgtttga tacggcttta
    13681 agggcgcacg cctaaaatat ggcagatggc gtaagtcagc tcgctgcggt taagcgtata
    13741 gaagtggaaa tctttcacgc cttcgcggga caggactttg accatatcga tggcgatact
    13801 tgccgccacc agattgcgcg taccttggtc gtcgtccaaa ccttcataca tttgcgacag
    13861 ccagcttggg attttgacgt tggttacttg cgccattttg ccgagctgct tgaagttggt
    13921 aacaggcaaa ataccgggga cgatttccac atcgataccc aacatcacgc agcggtcgcg
    13981 gaagcgcagg tagcgttcca catcgaagaa gaattgggtg atgacgtggt tcgcgcccgc
    14041 atcgattttg cgtttcagat tgatcaagtc ggcttgagcg gatttggctt ccgggtgtac
    14101 ctcaggatag gccgctacgg aaatgtcgaa gtcggcaacg gagcgcaaca gtttgaccaa
    14161 atcttcggca tagaaaggtt ttttctcgta gccgggcagc tcgtcgccgc gcagggcgac
    14221 aatgcggcgg atgccgctgt cccaatagtc tttggcgatt tggcgcaatt cgtcgggaga
    14281 agcgtcaata ccggtcaggt gcggggcggc ttccaagccg gtttcctgtt tgatgcgttt
    14341 gacgatgccg tgtgtgcggt cgcgctcgcc tgagtttgca ccgtaagtta cggaaacaaa
    14401 tttcgggtgc aaggtttgca ggcgatggat ggaatcccac agcatggttt ccatttgttc
    14461 gtttttcggc gggaaaaatt cgaatgaaac gttgatgtcg cctttcaaat cggaaaggct
    14521 gttatttaac gcattgattt cttttgcgta attcatggct gcgtaccttt tattatcttt
    14581 tatatgatgt ttcaggcaac ataataaagt tgcgccaaac atgagtcaat ttcaaaattt
    14641 tcagatttat catgaaaatt tctaatatat tacaaaaata gttaaatttc cttgtacagc
    14701 ccttctaaac tatgctagag tttgttatgt tataacactg aaagcaaggc aatatatgaa
    14761 accgaatatt catcccgaca attaccgaac cgtccttttt ttcgatagca gcgccaatga
    14821 aggctggtta atccgctcct gtgccgaaac acacggaaaa acgatggttt ggacagacgg
    14881 caaagaatat ccgctgtttt cgctggatac ttcttccgca tcccatcccg tctatactgg
    14941 caaacagcgc aacgtcaaca ccgaaggccg tgccagcaaa ttcaaccaac gtttccaatc
    15001 cgtaatgtca tcgtttagaa aggacaaata atgcaggttc tgtcttcact caaaaccgcc
    15061 aaacaacgcc accgcgactg ccaaatcgtc aggcgcaggg gcaaagttta cgtcatctgc
    15121 aaaagcaatc cgcgttttaa agcgcgccaa cgctgacccc ctgcccttcc tgcttcggtt
    15181 gtaaatcccc tcctaccgca agcccttttc aaaaaatgcc gtctgaaaca gaaaaatgcg
    15241 tttcagacgg cattttttcc gtcaatacga atccggaatg tgtttgaaat acagcataaa
    15301 cagataccaa aacagcgtcg cggcggccgc accgagcagc acccgggcac tgatttcggc
    15361 aacttcgcgc agggcgcgtt tctgcctgcg gctgaagagg cggttcataa tcagcgcggc
    15421 aagtgcgaaa aagaaaaaaa tacggaaaag tctgcctatc atgtcgggaa gcgaaaagta
    15481 tggttcgggc gcggcggcat ggggaaggat gtttcagcgc gtcaggctgc ttgtccgacc
    15541 gtgtcaaaaa gtcatgccgt ttgcgatata ataacgcttt ttgccgtatt tgttggaaca
    15601 ggatatgagt caaaatgcct cggtgggcat tgtaacgccc caaaaaattc cgtttgaaat
    15661 gccgctggtt ttggaaaacg gtaaaacttt gccgcgtttc gatctgatga ttgaaaccta
    15721 cggcgagctg aatgccgaaa aaaataatgc ggttttaatc tgtcatgcgc tgtcaggcaa
    15781 ccatcatgtt gcgggcaggc attcggcgga ggataaatat acgggctggt gggacaatat
    15841 ggtaggaccc ggcaaaccga ttgatacgga acgttttttc gtggtcggtt tgaacaatct
    15901 gggcggctgt gacggcagca gcggaccttt gtcgatcaat cctgaaacgg gtagggaata
    15961 cggcgcggat tttccggtgg ttacggtgaa ggactgggta aaatcacaag ccgcgcttgc
    16021 cgactatctc ggcatcgggc aatgggcggc ggttgtcggc ggcagtttgg gcggtatgca
    16081 ggctttgcag tgggcgattt cctatcccga acgcgtgcgc cataccttag tgattgcgtc
    16141 tgcgccgaaa ctgtccacgc aaaatatcgc gttcaacgat gtggcgcgtc aggcgatttt
    16201 gaccgacccc gatttcaacg aaggacatta ccgcagccat aataccgttc ctgcacgggg
    16261 cttgcggatt gcccgcatga tggggcacat tacctatctt gccgaagacg gtttgggcaa
    16321 aaaattcgga cgcgatttgc gttccaacgg ctatcaatac ggttatggcg ttgaatttga
    16381 agtagaatcc tatctgcgct atcagggcga caaattcgtc gggcggtttg atgccaacac
    16441 ctatttgctg atgaccaagg ctttggacta tttcgatccg gcggcggatt tcggcaacag
    16501 cctgacccgc gccgtgcagg atgttcaggc aaaattcttt gtcgccagct tcagcaccga
    16561 ctggcgtttc gcgcccgaac gttcgcacga actggtcaag gccctgattg ccgcccaaaa
    16621 atccgtgcag tatatcgaag tcaaatccgc acacgggcac gatgcctttt taatggaaga
    16681 cgaagcctat atgcgtgcgg tcgccgccta tatgaacaac gtttataagg aatgtcagca
    16741 atgaacctgc gcgatgattt gcaactgatt tacgaccgga tacccgaagg cagccgcgtc
    16801 ttggacttgg gctgcggcga cggcgaactg cttgccgcct tggtcgaaca caaaaaatgc
    16861 agcggctacg gcatcgaaat cgacaccgac agcgtgattg ccgcgatgtc gcgcggcgtg
    16921 aatgttatcc aagccgattt ggaagaaggt ttaaccgcat tcaacgatca aagttttgat
    16981 gtgattgtgt tgagccagac catccaagcg atgcagaata ccgaaaaaat cctgcgctgc
    17041 ctgatgcgcg tggcaaaaca ggcaatcgtc agcttcccga atttcggcta ctggcgcaac
    17101 cgcgtccaaa tcgcactcgg cggacatatg ccggtttccg aacgtatgcc ctaccattgg
    17161 tatgatacgc cgaacatcca ttggtgtacg ctcaaagact ttgatttgtt gtgcgccaaa
    17221 aacaacatac gcgtcctcga gcgcgccgtg atgacgggca acagacaggt caaacatttc
    17281 cccaaccttt tgggcagcct tgcgttttac cgcgtcgggt aagacagatt gagaaccttg
    17341 tcggcaataa caggttcttc ggttctatta acaacaggcc gtctgaaaaa tgaaatggca
    17401 ggttttcaga cggcctcttt aatattggaa acaatagaat gaaaaacaaa acctcatcac
    17461 ttctcttatg gcttgccgca atgatgctga ccgcgtgttc cccgagcaaa gaagataaaa
    17521 cgaaagaaaa cggcgcatcc gccgcctcgt ccacggcatc cgccgcttcg tcttctgcgc
    17581 cccaaaccga tttgcaaccg gccgcatccg cccctgataa cgtcaagcag gcagaaagcg
    17641 cgccgccgtc aaattgcacc gacctgcacc ccgccaccgg cattgacgat ctcatgcagc
    17701 aaatcgccga acacatcgac tcggactgtc tgtttgccct ttcccatcac gaactggaaa
    17761 cccgtttcgg cttacccggc ggcggctatg acaacataca gcggctgctg tttcccgaca
    17821 tccgccctga agatcccgac taccatcaga aaatcatact ggcaattgaa gacttgcgtt
    17881 acggaaagcg cacgatcagc cggcaggcac aagatgcctt gatggaacag gaacgccgcc
    17941 tccgagaagc gacgctgttg ctgatacagg gcagtcaaga aacccgcgga caaggcgagg
    18001 agccgaaacg cacgcgttat tttgaagttt cggcaacccc tgcctattcg agccggcaca
    18061 acaacggact tggcggcaat ttccaataca tcggccaatt gcccggctat ctgaaaatac
    18121 acggagaaat gcttgaaaac caatcactct tccggctgtc caaccgtgaa cgcaatcccg
    18181 acaaaccgtt tttagacatc cattttgacg aaaatggcaa aatcacgcgt attgtcgttt
    18241 acgaaaaaaa catctacttc aatccaaact tggggcgaag ataaaaaaat gccgtctgaa
    18301 acgttttcag acggcatttt tcaatgcaac aaatcaagcg cgtttgcgga attcgccggt
    18361 gcgggtatcg atttcgattt tgtcgccgtt ttcgatgtaa gacatcactt ggatttcggt
    18421 gcccccgacc aggcgcgcgg ttttcatcac tttgcctgaa gtatcgcctt tgacggcagg
    18481 ctcggtatac tcgacttcgc gtacgatgat ggtgggcagt tctacggaga tggggttgcc
    18541 ttcgtagaag gtaacttcgc attggtcttc cataccgtca acgatgaatt tcaacgcgtc
    18601 gccgatgttg tcggcttcga tttcgtattg gttgaattct tcgtccataa agacgtacat
    18661 cggatcggca aagtagctgt acgtacagtt tttgcgggac aggatgacca cgtcgaattt
    18721 gtcgtcggct ttgtaaatgg tttcggaagc cgcgccggtc agcaggtttt tcagtttcat
    18781 gctgactttg gcggaagagc ggccgccttt gatgtattcg gttttttgaa cgaccatagg
    18841 atcgttgccg accataaata cattgccggc gcgcagttct tgtgctgttt tcattgtgat
    18901 tttccaatca aatcggttgt cgtaaaacgc actattctag cgtatttttt gatgctttga
    18961 aacaaaggcg gcgagttttt cggatgcgga aggctgcccg aaaagataac ggctccaatc
    19021 ctccgcgcct tgccgccagc cgttttgatg ttgttgcagg atttgccaac attcgaggcg
    19081 ttgcgtggcg gataaagcct ctccgccgtt gaggtcgtct gaaaggcggc ggtgtaccga
    19141 tgcggtttcg ggcgagtaaa atccatgcgc cttatcccaa aaggcgtgga gtttatcgag
    19201 atggacattc tcgtcttgcg ggtagatgtg ccaaaagaag ggtttgccgg caagctgggc
    19261 gcgcacgaaa ctgtcttcgc cgcgtatcac ggcgcagtcg gcgaggtgca gcagtttgtc
    19321 gaagtcctgt tgcggcacga aagggatttt gacgaggcgg acggatgccg tctgaaaaac
    19381 atcgccgtcg ttttgcaggg catcttgcgg aataacgccg ctttgtttga ggctgtcgat
    19441 gatttgcgtc cccgccagca acagtgtcat cgggctgcct gcctgtcgcc acatttccag
    19501 ccactttgcc caaacatcgc tccgatagcc gaaaagcagc cattcggggg cgtttttttc
    19561 gggaagcatc agccgctctc gcaaggcttc ggtatcgaaa cggacggcat ttcggtaatc
    19621 gcgttcgcgt atcaacccgc cgcttttttc gctgaaaccc ataaaccaaa aatatttttg
    19681 aacactctcc tgcagcgaag gcatcagatg cagcctttca ttgctttctt ccgcgctcaa
    19741 atattcccaa ttcagccaaa gcggcttgtg tcggcggatg atgtgcagca cattttcggg
    19801 caggtcgcag gcaaaagttt cgataacgat atcgggaaca agggcagtat cgatatccgc
    19861 cgcatcggaa tgccaagtgc ggacatgaat atcctgatga acgcagggaa catcgggcaa
    19921 atcagggcaa agcgcacgca aggcggacac atcgtccgtc cacaaatgca cctgccaacc
    19981 gagttcgcgg tgcaaaacac gggcgagccg ccacgaaacg ccgatgtcgc cgaaattgtc
    20041 gatgaccttg caaaaaatcc aacagacaaa aggaggagta ttcatagaaa taacggaaac
    20101 aagctaaaac gcgccgaaat agtggaacaa atacacaatc agcaaaataa tcggaataaa
    20161 cagcaacata gtttgacgtt tcaaatcaaa ctcttgtttc aaatgatgct tgatggtgtc
    20221 gaaaaggacg aatttaaaca aaaacgtcgc ttcgcaggca aggatgatgc cgttgatcag
    20281 atgctggagt ttgtcttcgg gcggattggc ggcgaaaacg gcaagcgtcg ccaacgggat
    20341 gaggcagacg gcaagccaac ggacaagcag gtttcgagac attgcaaggc tttcaaaaaa
    20401 agcaaaacgc cattatcgcc ccaagcgcac gccttgtcta acggacgcac cggagatgcc
    20461 gtctgaagcc ttgccgcagg aaaacaccgc accgccgaac ctttaaatac gcccgagacg
    20521 ggcgttcccc gaaaaatcgg acgctgccgc cgatgcctga ggacaaggcg gcagcaggca
    20581 cgggcatatt gaaaacccgg cgcgtcggtt tgaa
//
LOCUS       DAWEWP010000021        20410 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWEWP010000021 DAWEWP010000000
VERSION     DAWEWP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20410
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.021"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            135..971
                     /gene="cysT"
                     /locus_tag="WGN53_000552"
     CDS             135..971
                     /gene="cysT"
                     /locus_tag="WGN53_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ0139576.1"
                     /translation="MLALKTPDVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     SGFWNTITEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGSGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            1226..2086
                     /gene="cysW"
                     /locus_tag="WGN53_000553"
     CDS             1226..2086
                     /gene="cysW"
                     /locus_tag="WGN53_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ0139577.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            2083..3156
                     /locus_tag="WGN53_000554"
     CDS             2083..3156
                     /locus_tag="WGN53_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ0139578.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGNILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKEQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(3213..4739)
                     /gene="ilvA"
                     /locus_tag="WGN53_000555"
     CDS             complement(3213..4739)
                     /gene="ilvA"
                     /locus_tag="WGN53_000555"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ0139579.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSHGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGDETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            4887..6056
                     /locus_tag="WGN53_000556"
     CDS             4887..6056
                     /locus_tag="WGN53_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ0139580.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWARLTGQ"
     gene            complement(6181..6753)
                     /locus_tag="WGN53_000557"
     CDS             complement(6181..6753)
                     /locus_tag="WGN53_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ0139581.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(6820..7803)
                     /locus_tag="WGN53_000558"
     CDS             complement(6820..7803)
                     /locus_tag="WGN53_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ0139582.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(7970..8045)
                     /locus_tag="WGN53_000559"
     tRNA            complement(7970..8045)
                     /locus_tag="WGN53_000559"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8010..8012),aa:Gln,seq:ttg)
     gene            complement(8054..8129)
                     /locus_tag="WGN53_000560"
     tRNA            complement(8054..8129)
                     /locus_tag="WGN53_000560"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8094..8096),aa:Gln,seq:ttg)
     gene            complement(8142..8217)
                     /locus_tag="WGN53_000561"
     tRNA            complement(8142..8217)
                     /locus_tag="WGN53_000561"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8182..8184),aa:Gln,seq:ttg)
     gene            complement(8225..9070)
                     /gene="ispE"
                     /locus_tag="WGN53_000562"
     CDS             complement(8225..9070)
                     /gene="ispE"
                     /locus_tag="WGN53_000562"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ0139583.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            complement(9080..9661)
                     /locus_tag="WGN53_000563"
     CDS             complement(9080..9661)
                     /locus_tag="WGN53_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ0139584.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPLVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGIPSETETPERCAARTR"
     gene            complement(9801..11639)
                     /locus_tag="WGN53_000564"
     CDS             complement(9801..11639)
                     /locus_tag="WGN53_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139585.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            complement(11804..12640)
                     /locus_tag="WGN53_000565"
     CDS             complement(11804..12640)
                     /locus_tag="WGN53_000565"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ0139586.1"
                     /translation="MQIIHTIQELRAWRKNAGTVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            complement(12597..12773)
                     /locus_tag="WGN53_000566"
     CDS             complement(12597..12773)
                     /locus_tag="WGN53_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139587.1"
                     /translation="MQTEQGLLPMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            complement(12763..13554)
                     /gene="panB"
                     /locus_tag="WGN53_000567"
     CDS             complement(12763..13554)
                     /gene="panB"
                     /locus_tag="WGN53_000567"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ0139588.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTVKFVKNFMQGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            complement(13696..13771)
                     /locus_tag="WGN53_000568"
     tRNA            complement(13696..13771)
                     /locus_tag="WGN53_000568"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13736..13738),aa:Thr,seq:tgt)
     gene            complement(13806..13881)
                     /locus_tag="WGN53_000569"
     tRNA            complement(13806..13881)
                     /locus_tag="WGN53_000569"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13846..13848),aa:Thr,seq:tgt)
     gene            14047..14838
                     /locus_tag="WGN53_000570"
     CDS             14047..14838
                     /locus_tag="WGN53_000570"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ0139589.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            14893..15534
                     /locus_tag="WGN53_000571"
     CDS             14893..15534
                     /locus_tag="WGN53_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0139590.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(15612..16535)
                     /locus_tag="WGN53_000572"
     CDS             complement(15612..16535)
                     /locus_tag="WGN53_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0139591.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGATLDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            16699..17568
                     /locus_tag="WGN53_000573"
     CDS             16699..17568
                     /locus_tag="WGN53_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ0139592.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDISAGISHTFLKDDKNPALIGFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPVVLSLTAAYRINGSKTLSSNTKYKAGNYWMLNPNISFAAN
                     DRISLTGGIQWLGKQPDRLDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            17611..18084
                     /locus_tag="WGN53_000574"
     CDS             17611..18084
                     /locus_tag="WGN53_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139593.1"
                     /translation="MKKQITSAVMMLSMIAPAMANGLDNQAFEKYVFHTQADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
     gene            18646..18918
                     /locus_tag="WGN53_000575"
     CDS             18646..18918
                     /locus_tag="WGN53_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139594.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIG
                     YVLIKNILVSVSDEDGTKYLAIYLSNLFFGTVLMYVLVKRLSKKPS"
     gene            19081..19626
                     /locus_tag="WGN53_000576"
     CDS             19081..19626
                     /locus_tag="WGN53_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139595.1"
                     /translation="MKKQITAAMMILTMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            19608..19973
                     /locus_tag="WGN53_000577"
     CDS             19608..19973
                     /locus_tag="WGN53_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139596.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            20031..>20410
                     /locus_tag="WGN53_000578"
     CDS             20031..>20410
                     /locus_tag="WGN53_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139597.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
BASE COUNT         5151 a         5505 c         4827 g         4927 t
ORIGIN      
        1 acatcgcgcc cgcttgagaa actgccaacc gctttataac aaattcgtct ttgcaccaaa
       61 ctttccattc tttccgtttt tcggacggca tcgttaaagt agtccttcct tttccttatt
      121 ttcagcattg ttttatgtta gcgctcaaaa cgcccgacgt actgcccggc ttcaaactca
      181 gccttggtct gaccgtattg tgcctgtcgc tgcttgtggt cttgccgttt gcgatgatgg
      241 cggcgaaggc ggcggaaatc ggctggagcg gattttggaa cacgattacc gaacccaatg
      301 tcttggcggc cgtctggctg agtttgcgga tgtcgtttta tgcgatgctg accaatgtcg
      361 tgttcggcac gctggtggcg tgggtattgg tgcgttatga attcccgggc aagggtctgg
      421 cgaacgcgct ggtcgatttg ccgtttgcgc tgccgacggc ggttacgggt atcgcgttgg
      481 cgaccctgta tgcgcccaac ggttggatag gccgtttttt cgagcctttg ggcatcaaaa
      541 tcgcgtttac gcccgtcggc atttggattg cgctggtcgt cgtcagcctg ccctttatcg
      601 tccgcgccgt gcagccggtg ttggaggaat tatcgggcga atatgaggaa gcggcggcga
      661 ctttgggcgc aaaccgcttt acgacgttcc gccgcgtgct gctgccggaa atcacgccgg
      721 cactcttgac cggctcggga atgatgttcg cgcgggcaac gggggaatac ggctcggtga
      781 tttttatcgc gggcaatatt ccgatggttt ctgaaatcct gccgctgatt attacgggca
      841 agctggaaca gttcgacgtg cagggcgcgt cggcggtggc gttgtttatg ctgctggttt
      901 cgtttgtgat tctgtttgcg ctgaacgtga tgcagtgggc gttgggcagg cgttcgggcg
      961 cgaagggttg aggtcgtctg aaatacctgt taccgtcatt cccgcgcagg cgggaatcca
     1021 ttggtgaatt tcggctgcct tatttatttt ctgttttcct gttgccctgc ggtggattcc
     1081 cgcctgcgcg ggaatgacgg tagctagacg tttttattcc cttaatcaat aaaaggttgt
     1141 ctgaaaacga atccgcccca caaaaaacgg tttttcagac ggcatccgaa caccttaaaa
     1201 caaccaaccg gggaacacca ccgccatgaa accctattcc gccaatccca acctgaccga
     1261 accgcgccgg ctgcgcgtgt tgctgattgc cgccgcgctg ggctttctgc tgctgatgct
     1321 ggtcgtgccg ctcgtcgccg tgttttacga agccttaaaa ggtggttggg atttgtacct
     1381 gaaatcctta aacgatcccg aagcgtggtc tgccatcaaa ttgacgctga ttaccgcgct
     1441 gattgtcgtt cccgtcaatg ctgtattggg tgtggcgatg gcgtggctgc tgacccgttt
     1501 tgattttcgc ggcaagcagt tgctgaccac cctgctcgat ttgccgtttt ccgtatcgcc
     1561 cgtggtggcc ggtttaatgt tcgtcttatt gttcggcgcg cacacggcat tgggtggctg
     1621 gctcgaagcg caaggcatac agattatctt cgccatcccc ggcattattc tggcgacgct
     1681 gttcgttact ttcccctttg tcgcacgcga aatcatcccg ctgatgcagg ctcagggcga
     1741 cagcgaagaa caggcggcat tgatactcgg cgcaagcggc tggcagatgt tttggcgcgt
     1801 taccctgccc aacatcaaat gggcgttact ctacggcatc atcctcacca acgcccgcgc
     1861 gatgggcgag ttcggcgcgg tcagcgtggt atcgggacac atacgcggcg aaaccaacac
     1921 cgtcccgctt ttggtcgaaa tcttctacaa cgaatacaac ttcaccggcg cattcgccct
     1981 ctccggcgta ttggcacttt tggcactggc gacgctggcg gtgcagaaca tcattaccaa
     2041 attacaagac aaaaaactcg ccgccgccga aaggaacgca gtatgagtat caccatccaa
     2101 aacttaaaca aacacttcgg caattttcac gcgctgaaaa acatcaacct caacgtcccc
     2161 accggcaaac tcgtttccct gctcggcccg tccggctgcg gcaaaaccac acttttacgc
     2221 attatcgccg gactggaaaa cgccgacggc ggcaatatcc tgtttgacgg gcaagacgta
     2281 accgccaaac atgtgcgcga gcgcaaagtc ggcttcgtgt tccaacacta cgcccttttc
     2341 cgccatatga acgtgtttga caacgtcgct ttcggtttga ccgtattgcc caagtccgaa
     2401 cgcccgtcca aagaacaaat ctgcactaaa gtcgaagaat tgctgaaact cgtacaactc
     2461 tcgcatttgg caaaatccta tccgcaccaa ctctccggcg ggcaacgcca gcgcatcgcc
     2521 ctcgcccgtg cgcttgcggt cgaaccgaaa ctcctgcttt tggacgaacc cttcggcgcg
     2581 ttggacgcca aagtacgcaa agaattacgc acctggctgc gcgatatcca tcacaacctg
     2641 ggtgtaacca gcattctggt tacgcacgac caagaagaag ccctcgaagt ttccgacgaa
     2701 atcgtcgtga tgaaccacgg caaaatcgaa caaaccggca gcgccgaagc tatttaccgc
     2761 aaacccgaaa atgccttcgt taccgagttc ctcggcgaaa ccgacgcttt tgaaggacgc
     2821 atcgaaaaag gcttctggca ttataacggc ttcgcgtgga aattggacgc acaatacaaa
     2881 tggcaggaac aaaccgccac cggctatatc cgcccgcacg aatggcagat cgccgccgaa
     2941 cacgaaacac cgatgatttg tgccgaaatc gaaaaagtcc acgccgtcgg ggcattgacg
     3001 catattttgg tgaaacacgg caaacaagac gtgcatatca cgctggctgg cagcgatgcc
     3061 gcgcgttacc caatcgccga aggcaaagaa ttgaagctga ttccgaaaca ggtttatgtc
     3121 ttctctcaaa acgaactgat tgaatattcg atttaaccat gaaagcgcaa tgccgtctga
     3181 aaggctttca gacggcattg tgctttcaag cgtcaggcaa gaaacagctt gtacgcggca
     3241 ttttgcgttt cctcgtgata gctgtatccc agactttcca agaaaccgtc aaatgcggcg
     3301 gcatcgtgcg gcggcacatc gataccgacc aaaatccgcc cgtaatccgc accgtggttg
     3361 cggtaatgga agagcgtaat gttccaaccg ccctgcatat ggttcaaaaa acgcgccaac
     3421 gcccccggac gctccggaaa ctcaaaactg accaaacgct cgttttctac tttgtccgtc
     3481 cgccctccaa ccatataacg gatatggatt ttggcaatct catcatcagt caggtcgaca
     3541 ttgggcaatc ccgcctcatc caaccggctg ccgataaccg ccaaatcctg cgggcctgcc
     3601 gcttgaagtc cgacaaagat atgcgctttt tcatcgtctc cgtagcggta gttgaactcg
     3661 gtaatattcc tatttcccaa tatattgaca aacttaagga agctgccgcg ttcttcgggg
     3721 atggtaacgg caaaaatacc ttcgttgccc tcgcccaatt cactacgctc cgacacatgg
     3781 cgcaaacggt gaaaattcat attcgcaccg ctggtaacgg caatcagggt ttggttttcc
     3841 gcgccttctc gggcgatata ggctttcaga cccgccaacg ccaacgcgcc cgccggctcg
     3901 gtaatgctgc gcgtgtcatc gaaaatatcc ttgaccgcgc cgcaaaccgc atcggtatcg
     3961 actgtaatga tttcatccaa aagttctttg cagaggcgga aggtttcgtc tccgacaact
     4021 ttgaccgcag tgccgtctga aaacagcccg acatctttca aatggacgat ttcacccgct
     4081 tcgaccgact gcttcataca gcaggaatcg ttggtctgaa cgccgataac tttgatttcg
     4141 gggcggacct gcttgataaa tgccgccacg cccgccgcca aaccgccacc gcctatcggt
     4201 acgaatacgg cgcggattgg atcgggatgc tggctgacaa tttccatccc caccgtcccc
     4261 tgtcccgcaa tcacatcagg atcatcaaac ggcgcgatat aggttaaccc ttctttttcc
     4321 gccaactcca tcgcataatc gtaggcatcg ttgtatgaaa cgccccgcaa aaccacctcg
     4381 ccgccatggc ttttaaccgc atccactttg attttcggcg tagtttccgg cataacgata
     4441 acggcacggc aacccaaacg ctgtgcggac aatgccacgc cttgagcatg attgcccgcg
     4501 cttgccgcaa tcacgccgca agcgagcgca tctttcggca acttggacat tttgttgtac
     4561 gcgccgcgta ttttgaacga aaaaaccggt tgcaaatctt cgcgtttcaa aaggatgttg
     4621 tttttcaaac gtacagaaag gctgcgtgcc ggttccaaag gcgtttcgac cgcgacatca
     4681 tagacagatg ccgtcaggat gcggatgagg taattggaat aaggaagggg cgtgttcata
     4741 attcaatatg ggataatcgg tttattaaaa tcgcaaaacc caaaaccata cgcccaagac
     4801 ggcgcgaaag caagaaaaat ccgcccgatc agacacccta agcgtataat cggcagactg
     4861 aaacacgcac acaattagaa tatttcatga cagcacataa aatcctgccc gtcctgcttt
     4921 ccatcatctt aggcgtttct cacgcaacgg ccgcatcgcc cgcgcccaac aaaccggcgg
     4981 tacacgccgc ccccacgttc caaacacccg aaaccctcac agcggcacac atcgttatcg
     5041 accttcaaag caaacagatt ttatccgcca aaaacatcaa tacccctgtc gaaccggcgg
     5101 cactaaccca actgatgacc gcatatctgg ttttcaaaaa catgaaatcg ggaaatatcc
     5161 gatctgaaga aaacttaaaa atacccgaat ccgcatgggc ttcagaagga agcagaatgt
     5221 ttgtacgtcc cggcgatacg gtcagcaccg acaaactctt aaaaggcatg attgcactat
     5281 ccgcaaacga tgccgcccta acccttgccg gccggctggg caacggctcg attgaaaatt
     5341 ttgtgcaaca aatgaacaaa gaagcccgac gcttgggcat gaagaacact gtattcaaaa
     5401 atccgacagg cttgagtaga gaaggacagg tttccaccgc caaagacctc gcccagctgt
     5461 ctgaagcatt gatgcgcgac tttccggaat attacccgct gttttccatc aaatctttca
     5521 aattcaaaaa tatagagcaa aacaaccgca atatcctttt atatagggac aacaatgtaa
     5581 acggtctgaa agccggacac acagaaagcg gcggctacaa ccttgccgtg tcatactccg
     5641 gcaacggcag gcacatcctt gtcatcacat tgggttcgga atcggcggaa acacgcgcat
     5701 cagacaacag caagctgctg aactgggcat tgcaggcctt cgatacgccc aaaatatatc
     5761 cgaaaggcaa aaccgttgcc caaatccaaa tttccggagg cagcaaaaaa accgtccgcg
     5821 caggcttcct caaagaagcc tacatcactc tgccacataa ggaagcgaaa atggcagaac
     5881 agattttgga aaccatacag ccgattcccg ccccggtaaa aaaagggcaa attttaggaa
     5941 aaatcaaaat cagacaaaac ggatacacca ttgccgaaaa agaaatcgtc gcactggaaa
     6001 atgtaaaaaa aagaagccgg tggcaaaggc tttgggcgcg tctgacaggg cagtaatctg
     6061 ctatttcaaa tatcccgttt ttccaacaaa taaagaaatg ccgtctgaaa cacggttcag
     6121 acggcataaa acaacagggc ggtacgtatt gcatacgcgc cgccctgctg ctgaaatcaa
     6181 ttagcgtttc ttaccggtaa cggtagcaac agccagattt tcgttacgtt tcagggaaac
     6241 gctttctaca ccttcaggca gtttgatgtc tgacaagtgc agaatgtcgc cggcaaccac
     6301 ttcagcacaa tccaaatcca agaaagcagg gatgttggca ggcaaagcaa ctacttcaac
     6361 agaagtgttt aacagagata cgcggccgcc ttgcagtttg accgcttggg aattttcagc
     6421 gttaacgatg tgcaggggaa cgcggatgcg tacgggttgg tctgctttca cagcttggaa
     6481 gtcgatgtgt tgaacttcgc ggcggaacgg gtgcatttgg aaatcgcgga cgataacgtc
     6541 tttggtttca ccgttcagag acaacttaat caacgcagta tggaaagatt ctttttccaa
     6601 tgcgtagaat acggttttgt gatccacagc gattgcaaca ggctcttgac cttcaccgta
     6661 cagaatgccg gggatttggc cttcgcgacg caggcggcgg ctcgcaccag tgccttgtgc
     6721 ttcacgaaca gaggcttgaa tttcataagt catgttaaat actccaagtt aggtaaaatc
     6781 gccgtcatcg gccgcgacca gcttaagacg gcttcgggct tatggcagca acatgctgcc
     6841 tgtcatcact tcttcattga aaagatatga gacggattct tcattgctaa tgcggcggac
     6901 ggtttcggcc aacagaccgg caatcgttac ctgacggata cggtcgcagt ttttagccgc
     6961 ttcagacaaa ggaatggtat cggttacgac cacctggtcg atttcggatg aggcgatacg
     7021 gctgaccgcc tctccggaga atacggcgtg gctggcatag gctagaacac gttcagcccc
     7081 ccgctctttc agggcgacgg cggctttgca cagcgtattt gcagtgtcaa tcatatcgtc
     7141 cacaatcaga caggttctac cttgaatatc gccgatgatg ttcatgactt ccgccacatt
     7201 ggctttcggg cggcgtttgt cgatgattgc caagtcggca ttcagggatt ttgccacggc
     7261 gcgggcgcgg acgacaccgc cgatgtccgg gctgacgacg gtcagatttt caatccgctg
     7321 ttgtttgatg tcgttcaaca gaatcggggt ggcataaata ttgtccaccg gaatatcgaa
     7381 gaaaccttga atctggtcgg catgcaaatc gacagtcaaa acacggtcga tccctgccga
     7441 atacagcata tttgccacca gtttggcaga aatcggaacg cggacggaac gcggacggcg
     7501 gtcttggcgc gcatagccga aatacggaat ggctgtggta atacgacctg ccgaagcacg
     7561 cttcagtgca tccgccatcg tcaggatttc catcaggttg tcattggtcg gcgcacaggt
     7621 cggctgaagg atgaaaacat cgcgcccgcg tacgttttcc aacagttcga cggcaacttc
     7681 gccgtctgaa aacttggata cggaagcatt gcccaaagaa atgtccaaat gcctgacaac
     7741 acgttgtgcc aattcgggat tggcattgcc tgtaaatacc atcaaactgt cgtacgcagc
     7801 catattctca cctgatttta tgtttaactt ccgctcagaa aacacaatgc ttccctctgc
     7861 ttgtaccaag cccgacatta tacctgcaac atccgacaca aacaaaggca tttcaatatt
     7921 tttatttcta tgaaataaaa gcgtgtaagc aggcttacac gcttttattt ggctggggag
     7981 gaaggattcg aaccttcgca tgctggaatc aaaatccagt gtcttaaccg cttgacgact
     8041 ccccaaaaag ggctggctgg ggaggaagga ttcgaacctt cgcatgctgg aatcaaaatc
     8101 cagtgtctta accgcttgac gactccccaa ctcgtttggc ttggctgggg aggaaggatt
     8161 cgaaccttcg catgctggaa tcaaaatcca gtgtcttaac cgcttgacga ctccccaaca
     8221 atgtttatac ggacaacaaa ggatgttttg aaagaccctc tgccaaatat gcctcgtaca
     8281 aacctgaaac ttgtcggtat atattgtatg cgctatccct atcttgacac gccgtgaata
     8341 cacacgcacc agaacctgtc attaaggcaa atccatatcg ggacaattcg gaataggctt
     8401 tccaaacttc agggtattct ttaaatacaa ctgcctgcat atcatttcta aacggttgca
     8461 ggttttggaa agtcggcatt atgcttgagg cggaatttcg tgtcaagcct tcgtgtgtga
     8521 aaatttttgc agtggaaacg tggacgggcg gtttgacgat gacataccac tgtttcggaa
     8581 tatccattcc tatcagtttt tcacctatcc cgcttgcaaa agcatttttt ccgaaaataa
     8641 aaaatggtac gtccgcaccc aaagttgcgc ccaagtcaat cagctgctgc gtcgttcggg
     8701 aacactgcca ccaacggttc aacaccagca aaacggttgc cgcatccgag cttccgccgc
     8761 ccaaacccgc ccctgtcggg atttttttgt ccagccatat ttccacgccg gcggggttgc
     8821 gcgcatattt ttgcagcaac gatgcggcac ggtagctcaa atcggcttcc tgcggcatgc
     8881 catcaaccgg attgtgcagg atgattttgc cgtcgtccct cggtttcaaa tatacggtat
     8941 cctgcaaatc tatcaagcag aatatgcttt cgatattgtg ataaccgtct tcccgcctgc
     9001 cggtaatcct caaatcgaga ttcagttttg caggtgcggg aaacgcctgc cgtccgtccg
     9061 caatattcat ctgtccgcct tatctcgtgc gcgccgcaca gcgttccggg gtttcggttt
     9121 cagacggaat accgatttcg gtgaaaacca gcctgatgtt caaatttccg ttattcagtt
     9181 gcaacgttcg gacttgcccc ccactgtcgg cggttctgcc gacagtccaa ccgtattgtt
     9241 ccaatatgcc gtccggcagg atgcggtaag gcgcgcccgc cacacgcctg ccatctgccc
     9301 agatatgcag atattggatt ggcagtttga aaccgaccag ctgcctgctc aattcttccg
     9361 cactttccgc ctgatagaca tttcctttgc cgtccactgc caatgcgccg tccctgtctt
     9421 gacacaactg cccgagcgta ctgcccaaag gggtattgat attgatggtt tccacgagcg
     9481 gttggtatgt ccaatcgaaa tttgcatacg aacctttccc ttccgctttc actgccaacc
     9541 gcccttctgc tgcaaaactg ctgatgtgtt cggacggctg ccacaggttt tcgttatttt
     9601 gaggtaattg cgcacaagcg gtcaaaagca ggatgaccga tgcggatacg gtgtgtttca
     9661 tcagaatttc cttaacgggt gccatcctgc cggccggcgg gttcggcgtg ccgtgcaaaa
     9721 cgcgtgcgaa ctgcgtgcgg tttgcccgaa tgccgcagct tcccggcaaa ccttatgccg
     9781 tctgaaagga tggacctgca ttatttccgg ggtttgcggg aaggttgggg caatgcgatg
     9841 ccgtgacgtt tgagcgtttc ccgccatatt ttcttgtctc ccgtaaggtg tgccgcctgc
     9901 gtccatacgt caaccgcctg atcgcgttcg cccaatgccc acaacacttc gcccaaatgg
     9961 gcggcaactt cgggctcggg gtcgttttca aacgaatacc gcagatacgg cagcgcgctt
    10021 tccgcgtcgc ctttcaggta atacgcccag cctatgctgt cgttgacagc ggtatcgtcc
    10081 gggttgattt ggtatgccgt ctgaagcagg gcgaagcctt cgtccaaacg tttggaatcg
    10141 gaaagcaggc tgtagcccag attattcata atctgagcgt tatcgggtgc aagcctgaac
    10201 gccctttcaa gatctgaaat catttttttc cgcttgccaa gccgatcgta aacaactgac
    10261 cgctgtacca atgcctctgc ctgtaactct gtattactgc cggcaggcgg tttttcgata
    10321 atcttgtcca accccctcaa agcctcccgt ttgtcgggca gcttcgacag ggcgaacatc
    10381 tgtattttgg acaaattgtc tgccgtaaaa taccgcccct gctgttcggg aagtttccgc
    10441 accctgccga tctgccgcaa agccgccctg ccgccgtcca actcgacagc cgccgcagcc
    10501 gccagcacac ctttgtcgaa caggtattcc ggagcggaca cttttttcaa ccactgcctg
    10561 actttggtgt aatcccttcg gtcggcatat atcatcgccg ccgtcattgc cgccctgccc
    10621 cgctgttccc ccgtccccct gccgtatgcc ttttcggcgt agccgtcgat aacggaagca
    10681 ccttcttttc ggtttgccgc caatatcgct gcctgaatat acaggtctgc attcggattg
    10741 cgttccaaca gcacgttcaa acgcgcatag gcatcatcca gcctgtgcag ggaaaccaga
    10801 ttcataattt ccatttcctg ccagacggcc gaaaggtttt gggtgtctgt ctgctcgaaa
    10861 aagccgtcga gtatttcggg atatttgcgt gcagtcagac gcaacgtcat taaagtgggg
    10921 ggcaatattt ccgtatcgag cttcgccaaa cgctgcaaag ctccgattgc cttttccttt
    10981 tcgcgtccct gtacgctgaa caccacatcg gcaaccgccg cttcgggcag atgttcatat
    11041 ttcaacgccg cacggcgaac cgctttcgat gctttttgcg ccaacccgtc ctgttgcacg
    11101 gcggcttgtg ccaacaataa aaacaccctg cggttctgtc cttcgtccgc ctgagccagc
    11161 acttcttcca gtccgtctag atgctgattt cctctttccc tcagcacgtt ccgcagccac
    11221 cccgcccgtt tttgcgcctt acccggtata ggctcaatct gccgccattt ctgataaatc
    11281 atttccgcct gttcaaacgc gttcagcgac acggccattt ccaaggcgcg ttcggcgact
    11341 tcgggggatt ttgtgcgttc caacatcagc atataggttg ccagagccgt tcccgcctgc
    11401 cccttttgca aggcggtttc ccctcccagc aacgtaaata tctgattaac ccgctcgccc
    11461 actgccgcaa gccgtgcgcg ttcgtttttg atttcttcct cgctgtaacg ctgctgcttt
    11521 ctgaaaacct ttccgacttc cttcggcggc ttcgcatccg ccgcgccggc ggcatacgcc
    11581 tgcccggcaa gcagggctgc cgcgagcaca gataaaatgg tgaaacgggc gggtaacata
    11641 atcttccttt atccgacagc cgtccgaacc ttcagacggc atataatcag gcgtaacttt
    11701 atcacaagca gaatcgtttg tagatttgcg aaaccccgtt caaaaaaata aatgccgtct
    11761 gaaacgcaaa tccgcttcag acggcattcc cgacgcttgc ggtttaaggg agttttattt
    11821 ccaaattgtc aatcaggcgc gtcgtcccca gacgggcggc ggccaagacc accagtttct
    11881 tatctcccgc ccgcgccact tcgagcgtat cggcgcggcg gatttcgaca taatcgacca
    11941 cccagcccgc agcggtcaaa tggtcggcag cctgccgctc caaacctgca taatccaaac
    12001 tgccctgcgc caaggattcg gcaacagcct ttaattcgcg gtacaggcgc ggtgcttcgt
    12061 cgcgttccgc cgcactcaaa tactggttgc ggctggagag tgccaaaccg tcttccgcgc
    12121 gccccgtatc gacaggcact atttcaacat caaaattcaa atcctcggtc aaacctttaa
    12181 tcacggcaag ctgctgatag tcttttttgc cgaaacaggc aacatccggc gcgacaatat
    12241 tgaacagctt ggacaccacc gtcgccaccc cgcggaaatg tcccggacga aacttgccgc
    12301 acaactcatt ttgcagattg ggcggttcga cgttgtaacg ctgttccacg ttcggataga
    12361 gttctttctc atcgggcgcg aaaacaacgg caacaccctc ggcttccagc ttgtccgcat
    12421 cctgttgcaa agtacgcgga tatttgtcga aatcctcgcc ctgaccgaat tgcaggcggt
    12481 tgacgaatat gctgacaaca acgttgtcgg cgcgtttttt ggcttcgcga acgagggcaa
    12541 gatgcccttc gtgcagattg cccatggtcg gcacaaatgc caccgttccc gcatttttac
    12601 gccacgcgcg cagttcttga atggtatgta tgatttgcat aacggtaatc cttatccttc
    12661 gggcttcctg cccgacaaaa cgcgccgatt atacgcgccg cgcacggcaa aacaaaatgc
    12721 cgtctgaaac ggctttcaga cggcatcggc agcaaacctt gttcagtctg caaaaatatg
    12781 ttccgcagca gggaaggttt tggctttgac ttcggcaaca tacgcccgaa ccgccgcctg
    12841 aatgctgctt tgcccctgca taaagttttt gacgaatttc accgtcttac ccgggaaaat
    12901 gccgagcata tcgtgcatca ccaaaacctg cccgtcgcaa tcgacacccg cgccgatgcc
    12961 gatggtcgga caggaaacgg attcagttac cttttttgcc agttccgcag gaacacactc
    13021 catcagcacg accgccgccc ccgcatcatc atgcgccttg gcatcgttaa gcaacgcctg
    13081 cgccttgccg ccgcgcccct gaaccttgta tccgccgaag gcaaacacgg attgcggggt
    13141 cagaccgatg tgggcacaga ccggaatacc gcgcatttgc agaaattcgg tcgtttccgc
    13201 catccacaca ccgccttcga gtttgaccat atgcgcgccg gcagccatca gttcggcggc
    13261 ggcggcaaac gcctgctcct tactctgctg atatgcacca aacggcaaat cgctgacaat
    13321 catcgcattt tttgcaccgc gtgctacaca ttcggtgtga taacacatat cgcgcaggct
    13381 gacgggcagc gtcgactgcc gcccttgaac cgccattccc aaagaatccc caaccagcag
    13441 cacatccacg ccggcatcgt ccatcagcgc ggcaaaactg gattcgtaag cggtcagcat
    13501 agcgattttc tcgcccgccg ccttcatttt ttgcagtgtg ttcacagtaa tcatcagata
    13561 ttgttacccc gcccttttca gacggccttt tcagtttgaa acgggctatt atagtgaaat
    13621 gcccgcagcc ctgccaccga ccgtgccgca aatgcaaaac agcccgactg ttttacacaa
    13681 tcggactgtc aaatctggtg ccggcaccaa gagtcgaact cgggaccccc tgattacaag
    13741 tcaggtgctc taccaactga gctataccgg cttactgaaa tgttttgcaa caccatcagc
    13801 agaaatggtg ccggcaccaa gagtcgaact cgggaccccc tgattacaag tcaggtgctc
    13861 taccaactga gctataccgg caaagaaagc gaattatgca ggcaacttca cactttggca
    13921 aggaatttat ccgaaagccc cggcaaatcc gaataagtaa tttatttgaa aagaaattaa
    13981 tttgaagcag gacaagcatc ggcattctga taaaatatcc gcctttcaaa ccgaccgttc
    14041 acaccgatgg caagacatcc ctaccgccgc ctgcgtcctg caaaatccgg ctttcccgaa
    14101 gtcggcattt ccgaagaagg caatatccgt tcgcttcact tgggcagcga caccgttcaa
    14161 agctcaatga acctcgacca cccgtccgag ctggtactct cttacagccg cgcgatgatg
    14221 ggctggctgc tgtttaccga cgcacttccg cagcatatca cccaaatcgg cttgggcggc
    14281 ggctcgtttg cacgctggat agacacctac ctgcccgaca cgcgccaaac cgccgtggac
    14341 atcaatcctc aggtcatcgc cattgcccgc aacctgtttg agttgccttt cgagggcgag
    14401 aaatttgaaa ttattgaagc agacggtgca gaatatatca aagtcttccg ccacaacacc
    14461 gatgtgattt tggtggacgg gttcgacggc gaacaaatca tcgatgcgct ggttgaagaa
    14521 ccgtttttcc gagactgccg aaacgcactc tcttcagacg gcatattcgt aaccaactgg
    14581 tggagcggcg acaaacgcta ccaacgcttc atcgaacggt tgttgagcgt ttttgaaggg
    14641 cgcgtcttgg aacttcctgc cgaaagccac ggcaatgtcg cggtaatggc cttccaaagc
    14701 agccccaaag agcaaaacat agacaaactc aaaaaacgtg ccgacaaact gagcaacgca
    14761 tacggattgg acttccaccg tatgcttgcc ggcctgaaag cgtccaaccc caacaacggc
    14821 aagcatttcc acctttaagc aactatcttc acggctgcat tggaacatgc ggaaaatcaa
    14881 gaggaaatca ttatgctgaa acccgaaacc atacacatat ccggtcctgc cggcatttta
    14941 gaaaccatcc atatcccgtc cgaacaagta ccggcacgcg gtgttgccgt catcaatcat
    15001 cccaaccccc tccagggcgg aacaaacacc aacaaagtta tccaaactgc cgccaaagcc
    15061 ttaagcaaac tcggcttcca ctgctaccta cccaaccttc gcggcgtagg cggcagcgaa
    15121 ggcacacacg attacggacg cggcgaaacg caagactgcc tcgccgtcat cgactatgcc
    15181 cgtgcccaac atcccgaagc ccccgaattt gccttatccg gcttctcctt cggcggttat
    15241 gtcgccacat ttgccgcaca agcgcgcata cccgatttat tgctgctcat cggcgcggca
    15301 gtctgccact ataccggccg ccccgagccg tccgccgttc ccaatgttgc caaaacgctg
    15361 atgatacacg gcgcggaaga cgaagtcgtc gaaatcggga aagcactgaa atgggcggaa
    15421 ccgcaagatt tgcccgtcat taccatcgcc ggttccacgc atttctttca cggcaaactc
    15481 atcgtcctgc gcgacaccat cctccgcttc gcgcccgtat gcctgaacgg ataaaacccc
    15541 tttcagacgg catcgataat gtaaaacccg tatcggcggc atccgccgat acgggtttgc
    15601 gttccctctc attataggca aaatccgctg caaaacaccc tttcccgacc cgacaaagga
    15661 tctgcaaacg caatcttttt tgccaatagc ttcaaaggtt tccgataatc ctcgctgccc
    15721 gcttcagacg gcacgggata gagcgcgtca ttcaacagcg gcatacccaa acccatcata
    15781 tgcacgcgca actggtgttt cttgcccgta tgcggcgtaa ggcggtaaag gctgaattcc
    15841 cccctgtttt caatcagttc gaccgtcgta tgtgcgtttg gttcgccttc cgcctcttgc
    15901 gtcgtaaaaa atttctcacc cctcaccaaa cgcgaaacca catcgagcgg atacggcaaa
    15961 tccgtccttg tcggcgcaag cgcctcatac gttttccata ccgttttgtt ttgaaacatc
    16021 gtctgatagg ctccgcgcgt ggcaggatta tgcgacagca gcatcacgcc tgccgtatcc
    16081 ttgtccaagc ggtgcagcgg cgtaatgtcc tcaacattca aatgctgcaa ttcaggccgc
    16141 aaacgcaggc gcgtgagcag ggtttcccgc aaaaacctgc cgctggggat gacgggcaga
    16201 aaatgcggct tgtccaccac aatcaaatgc tcatcaatat gcaaaatctt ttcttcaaac
    16261 ggaatacgcg gctcatcctc actgctggtt tcccgataat aaaacatcac cttacccggc
    16321 tcgaacaaag tatgttcgtc caacgtcgca ccatccgaac cgaccacaaa accgctgttc
    16381 aaccgcctgc gccaatcgtc cgcgcccaca aaaggaaagc ggatgcacag aaaatgcagc
    16441 agcggcagcc cgtaaaactg cttttcatgc ggcagcacca aataactggg tttgacaccg
    16501 ttcaacagag gaagaggatt attgcgtttt ttcatgcggc ggattgtaac cgtttttcac
    16561 aaacttttca gacggcatat cgaaacataa ttgacaaaaa caaacatatt gttttttatg
    16621 atttaaaaat ttatttagac aagattatcg gatattcatt attatcagta aataagaatt
    16681 aatatcaata ggagaaatat gaagcgcatc tttttgcccg ccttgcccgc catcctgcct
    16741 ttatccactt atgccgacct gcccttgacg attgaagaca taatgaccga caagggcaaa
    16801 tggaaactgg aaacttccct tacctacctg aacagcgaaa acagccgcgc cgaacttgcc
    16861 gcaccggttt acattcaaac cggcgcaacc tcgtttatcc ccattccgac cgaaattcaa
    16921 gaaaacggca gcaataccga tatgctcgcc ggcacgctcg gtttgcgcta cggactgacc
    16981 ggcaataccg acatttacgg cagcggcagc tatctgtggc acgaagaacg caaactcgac
    17041 ggcaacggca aaacccgcaa caaacggatg tccgacatat ccgccggcat cagccacacc
    17101 ttcctcaaag acgacaaaaa ccccgcccta atcggctttc ttgaaagcac ggtttacgaa
    17161 aaatcgcgca acaaagcctc gtcgggaaaa tcgtggctca tcggtgccac cacctacaaa
    17221 gccatcgacc ccgtcgtcct ctcattgact gctgcctacc gtatcaacgg cagcaaaacc
    17281 ctttcaagca acaccaaata caaagcaggc aattactgga tgctgaatcc caatatatcc
    17341 ttcgccgcca acgacagaat cagcctcacg ggcggcatcc aatggctggg caagcagccc
    17401 gaccgtctgg acggcaaaaa agaatccgca agaaacacat ccacctatgc ccatttcggc
    17461 gcaggtttcg gtttcaccaa aaccacggct ttaaacgcat ccgcacgttt caacgtttca
    17521 gggcaaagca gttccgaact gaaatttggc gtacagcata cattttaagc aggttttgat
    17581 tttccaacct tgtaatccaa aggagttatt atgaaaaaac aaatcacctc agccgtaatg
    17641 atgctgtcta tgattgcccc cgcaatggca aacggattgg acaatcaggc atttgaaaaa
    17701 tacgtattcc acacgcaggc agatgcgccg atgcagttgg cggagctttc tcaaaaggag
    17761 atgaaggaga ctgagggggc gtttcttcct attgtggcag gtgctttgat gggaggggca
    17821 attagtgctt gggctaatca cggcatttct aaaattaaaa cagggcaatt tgcttcaaca
    17881 agaagtacct tggctgcaac cgctggtggt atgataggag gcggatatag cagtgcgatg
    17941 ttgcgcggtg cagggattac tactagtgtt tttgcccgtt ctgcttggaa agggggaaat
    18001 gctattccta atgcagttat tcgtgcaaat ggtgcagcat taaatcagtc ttctagcgcc
    18061 gcaatgggaa aacatagaag ataaacgaaa tggatgatct aatactatat tttttatcag
    18121 gtatattcgg aaatcaagtt gctgaatata ttataaaaaa caaccgtgag ataaaagttc
    18181 cctttattgt gctttacgca atatttttta cgttgattta tactgtggct ttgttgtttc
    18241 tcagtctgat ctattgggtc aatggtgcgg agattgcatg gaaggggata ggtattttca
    18301 gtatgtcggt cagtttttgt atagtcttct gtctttattt gattgacaag gcaggaagat
    18361 gtaaggataa gaaacaatag ggatcagatg cggcggcaaa cgtagattgg gtctaggctc
    18421 aacctgcgtt tgctgcatct ttggaagaaa tagtggattc gaaaaaatag gaagaattct
    18481 gattaatggt atgcgaggtg tagcaatggg ggctgttgct ggtggcataa atggttatgc
    18541 aggttctacg ggtaagaaca ctgacattcg ccgttaaata ataaataaac caacttccaa
    18601 ccaaacctgc tatcggtttg gttggaagtg agaggaggta aggtagtgtt tgctgttatt
    18661 ttcttttcga cattgggctg tattttagca tggataagag atattcctaa aataaaatca
    18721 aaaaaaattt tggcaagatc cttatatatt ataggaataa taaatgtaat aatcggctat
    18781 gtattaatta aaaatatatt ggtttctgtt tcggatgaag atggaacaaa atatcttgca
    18841 atatatttat caaatctttt ttttgggaca gtattgatgt atgttcttgt gaaaagatta
    18901 tctaaaaagc caagctgaaa cccagtacac aactcatcaa taaaaatccc ttttgcacgg
    18961 aacaatgaca atattcaaat tgctggattt gcaatctgat ttacctattg aaattgggtg
    19021 tgcaacatac gttctaagca gggctttgat tttccaacct tgtaatccaa aggagttatt
    19081 atgaaaaaac aaattaccgc agcgatgatg attctgacaa tgattgcccc cgcaatggca
    19141 aacagcatgg acaatcagat atttgaaaac caagtattcc atacgcgggc agatgcgccg
    19201 atgcagttgg cggaactttc tcaaaaggag atgaaggaga ctgagagggc gtttcttcca
    19261 ttggctatct tgggtggtgc tgccattggt atgtggacac agcatggttt tagttatgca
    19321 acgacaggca gaccagcttc tgttagagat gttgctattg ctggcggatt aggcgcaatt
    19381 cctggtggtg taggtgccgc aggaaaggtt gtttcctttg ctaaatatgg acgtgagatt
    19441 aaaatcggca ataatatgcg gatagcccct ttcggtaata gaacaggtca tcctattgga
    19501 aaatttcccc attatcatcg tcgagttacg gataatacgg gcaagacttt gcctggacag
    19561 ggaattggtc gtcatcgccc ttgggaatca aaatctacgg acagatcatg gaaaaaccgc
    19621 ttctaactcc tccttttttt ctatttgaag atgtgtcttt ggatatattt caaggattgt
    19681 cggaattgga aaagaaaata gagccgcagg atttgatgga tgatgtttac cgtgcttttg
    19741 attcggttgg aaatattctg aatttccgta tagtagaaaa agagcaaaaa gggttttggg
    19801 taagtaccaa aatcaaaaca gttgtatttg attcggcaga tatgtcttct gatgatcttt
    19861 ttctgaaatg cttacagtct tcatataagg cttattttga aactgaacct gccggtttag
    19921 ataaacggca gctgatgaaa acgttaattc aaaaatgcgg attcagttgt tgacgaaaaa
    19981 atggattctg aactaacaga aactgatttc gaccatgtat gaggtaaaaa atgtctttcg
    20041 gctatttgat tgcaacatcg caaccttgcg aattgttgac aaaatctcga ggtgaaactt
    20101 tttctttaat tatggataag atggatttat ggatttattt cagattttgt gaaggaaata
    20161 tttatacaat aaggaagaat gaaactgaat cttgtcttac agaaagagga ggcaaatggt
    20221 tgaaacatat ttatgaattt aatcggggaa gttttatttt ttcttatgtg cttctaaaaa
    20281 agagagaaag cgaagagaat tttgcagaaa ttgttttaaa atcaataaaa aataataaaa
    20341 ttttgacagt taaggtacgg tcaggcttgc attttgactt aagaaatatt tatcgtattg
    20401 agatgtatag
//
LOCUS       DAWEWP010000022        20386 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWEWP010000022 DAWEWP010000000
VERSION     DAWEWP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.022"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            238..354
                     /locus_tag="WGN53_000579"
     CDS             238..354
                     /locus_tag="WGN53_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ0139598.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELGV"
     gene            complement(697..1212)
                     /locus_tag="WGN53_000580"
     CDS             complement(697..1212)
                     /locus_tag="WGN53_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139599.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(1278..1691)
                     /locus_tag="WGN53_000581"
     CDS             complement(1278..1691)
                     /locus_tag="WGN53_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0139600.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRALSPPFTGINIRLFAALGLLSL
                     SGAAAQASVYHCNSNGKSVYTSAPAGSCADADLPKISSHQGGGYRLKIKKLSQEAKIH
                     IESKKKNKKPAGKKNRQAAKAPKENQKQPTEKKSP"
     gene            complement(1678..3600)
                     /locus_tag="WGN53_000582"
     CDS             complement(1678..3600)
                     /locus_tag="WGN53_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0139601.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPKVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMVQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(3677..4072)
                     /locus_tag="WGN53_000583"
     CDS             complement(3677..4072)
                     /locus_tag="WGN53_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139602.1"
                     /translation="MKRQQDKKMLFEENPIDEHFAEYDRRTGEIRLSGRHFNKPVLLH
                     RDSAGLSKWQSLSELTPDNLLSDVKADDYPEILIIGTGAAQEFIHPKIMADFSRIGIG
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            4246..5553
                     /locus_tag="WGN53_000584"
     CDS             4246..5553
                     /locus_tag="WGN53_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ0139603.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREVRISAVCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGHDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            5546..5980
                     /locus_tag="WGN53_000585"
     CDS             5546..5980
                     /locus_tag="WGN53_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139604.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLTVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(6039..6308)
                     /locus_tag="WGN53_000586"
     CDS             complement(6039..6308)
                     /locus_tag="WGN53_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0139605.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(6490..8940)
                     /gene="lon"
                     /locus_tag="WGN53_000587"
     CDS             complement(6490..8940)
                     /gene="lon"
                     /locus_tag="WGN53_000587"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ0139606.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTGAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHIHVP
                     EGATPKDGPSAGIAMTLAAVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            9191..10294
                     /locus_tag="WGN53_000588"
     CDS             9191..10294
                     /locus_tag="WGN53_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0139607.1"
                     /translation="MSSFIFPDNGAEWRNESVQKPPKGWHYVRESGADGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRARKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLGLRPADFLEKAFIRAGLRVADMM
                     KTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
     gene            complement(10322..12067)
                     /gene="recD"
                     /locus_tag="WGN53_000589"
     CDS             complement(10322..12067)
                     /gene="recD"
                     /locus_tag="WGN53_000589"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ0139608.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSGSEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQPFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGEAHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNKAYCSHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPDFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(12135..12830)
                     /gene="lolD"
                     /locus_tag="WGN53_000590"
     CDS             complement(12135..12830)
                     /gene="lolD"
                     /locus_tag="WGN53_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ0139609.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGILRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKKREEAAETAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(12823..14070)
                     /locus_tag="WGN53_000591"
     CDS             complement(12823..14070)
                     /locus_tag="WGN53_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ0139610.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            14275..14553
                     /locus_tag="WGN53_000592"
     CDS             14275..14553
                     /locus_tag="WGN53_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139611.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(14609..15226)
                     /gene="recR"
                     /locus_tag="WGN53_000593"
     CDS             complement(14609..15226)
                     /gene="recR"
                     /locus_tag="WGN53_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ0139612.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(15293..16831)
                     /locus_tag="WGN53_000594"
     CDS             complement(15293..16831)
                     /locus_tag="WGN53_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ0139613.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAMQNEQADGGSPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(16886..17260)
                     /locus_tag="WGN53_000595"
     CDS             complement(16886..17260)
                     /locus_tag="WGN53_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ0139614.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAGTQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGRV
                     YVGFSEETYDGIFNRQALCRQG"
     gene            17410..19038
                     /locus_tag="WGN53_000596"
     CDS             17410..19038
                     /locus_tag="WGN53_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ0139615.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            19110..20363
                     /locus_tag="WGN53_000597"
     CDS             19110..20363
                     /locus_tag="WGN53_000597"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ0139616.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYTLARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWFALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKTFRAAQDKTEKH"
BASE COUNT         4555 a         5492 c         5365 g         4974 t
ORIGIN      
        1 atgaagcaaa aacgccttaa agtccaactt aagctgctca ttgatgaaaa agggctggat
       61 attgatgcag tgtttgagct gattatcagg cacgcggaat acggagctta atatgaaaat
      121 cggcaatcta aatgtagaaa tccgcaggaa agcgattaaa aacctgcaca tcagcgtgtt
      181 gccgccaagt ggcgcggttc gggtgtccgc accgcttaat acgtcgtcgt ccacgaactg
      241 acccatttgc tcgaacgcca tcgcaacgcc cgttttatgg cgcatatgga caactttctc
      301 ccaaactggc aaagcatcaa acaacagctt aatgccttgg agttaggggt gtaacgcaag
      361 gacgaatccg aagcagaccg tttgcacaaa tatataattt aacataatat acattatgcg
      421 aactatcgga aacagttgta cgtgtccctg tggtctttcc aagtaggaaa attaaagtat
      481 ggccaatgcg gctgaatgta tagcccggag catccgcgta tccgaaggta ctgaacgaca
      541 tgccctgcat ccctttcgac gcttccatcc agccggtatt gggaaatacg ttgaccttca
      601 atataatata caggcagccg acacacagga tatgccgctg ctttttcatc atttcttctg
      661 tcaaatcctt ggaacggtcg atttgaaaac acggctttac atacgcccca gttccctttg
      721 cagggcttga atattttgct gcctgtccaa tacattgctt tgtaatgcat ttatttcttg
      781 atggttgatg ttgccgccct ttgccagacg tgcttgtgat aacatttttt gggcttcaac
      841 caatgctttg cgttcgttgc tcaattctgt ttcgagaatg gagcgtctgc tgttgtttga
      901 gggtgcttgt tgcggcggcg gcgtattgga ttttgccggc ttggatactg ttttgaccgg
      961 ggctttatat ttgacaacct gtccgccgtt tgacggtgat gataccggtt cgggcgtttg
     1021 gggcgggata tagcgttcgc tgctgtagtt gccgattggg ggcaaatcgg ttgagtggca
     1081 gcttttggac ggcttggtgg tgtaaacggt ttctccgttg attgtgcagg tgtagatttt
     1141 ggccgcattc gcacccaatg ggcttgaaat cagggaaaag ttgattagga ttaaggggag
     1201 ttttgatttc ataatgtgct tgattttcgg ataatcattt gattttttgg tattttttat
     1261 aatgctttct tggcgtttta cgggcttttc ttttcggttg gttgcttttg attttctttc
     1321 ggggctttgg cagcctgcct gttctttttc ccggcaggtt ttttgttttt ctttttactt
     1381 tctatgtgta ttttggcttc ttgactgagt tttttaattt tcaggcggta tccgcctccc
     1441 tgatggctgc tgattttagg taaatccgca tcggcgcacg atcctgctgg ggctgatgta
     1501 tatacgcttt tgccatttga gttgcaatgg tatacggagg cttgcgccgc cgcgccggaa
     1561 agggacagga gacccaaggc ggcaaaaagt ctgatgttta taccggtaaa aggcggtgat
     1621 aacgcccgaa ttataccgtt attggcaggc aaagataagc accctgcccg cgcttcttta
     1681 tcgctcggca aactgtttct cgatttcagt ttcaatccgt tccaattctt cttgacaagc
     1741 cagccaaacc tcttcgattt gggcaagttg tgttttgact tttgccagct gggataaggt
     1801 attttgcaat ttttctttgt ttgcttcaag gtaagcgtct tcttgtgcta aaaatgcttc
     1861 acatgccgtc tgaatttcgg aaagctgcac catttctttt tcggcacggt ctattttctg
     1921 ctgtatcggc ttgccgcgtc gggctttttc ctgacggatt tgcgcttcga tgcgcttggt
     1981 gtctttgcgg ctttggcttt gtgcggatgc tgcgggtgcg gcggcggcgt tttcctgtgc
     2041 caaacgccat tggcggtaat cgttcaaatc gccgtcgaag ttcttcagac ggcctttgtc
     2101 gatcaggagg aagctgtcgg tagtcgcttc aagcaggctg cggtcgtgcg atacgatgat
     2161 taaggcgcct tggaaacttt gcagagcgag cgtcagggcg tggcgcatat ccaagtccag
     2221 atggttggtt ggttcgtcaa gcagcaacag gttcggcttt tgccagataa tcatggcaag
     2281 ggcaaggcgg gctttttctc cgccggaaaa aggttcggtt ttctgtaacg ccatatcgcc
     2341 aacaaaattg aagccgccga ggaaatttcg gatttcttgt tcgcgtactt tgggagaaag
     2401 ctgctgaata tgccaaacag ggttttggtc ggcgcggatg gtgtcgagtt ggtgttgggc
     2461 aaaatagccg atattgagtt tttcggaacg gacgatgctg ccggaaagca aatcgatttt
     2521 gcccgccaag gctttgataa aggtagattt accgctgccg ttgacaccca atagcccgta
     2581 gcgtgcgccg ctttccagcg acagggtaat gtcatgcaga acggttttgc cttcgtaacc
     2641 caaatcagcg tgttctagct ttaacaaagg attgggcaga tggtcgggat ggtaaaactc
     2701 aaaggaaaac tcgctgtcca gatgcgcggg cgcaacgcgt tctagctttg ccaaagcctt
     2761 catgcggctt tgcgcttgaa cggctttggt ggctttggct ttgaagcggt cgataaagga
     2821 ttgcagatgt ttgatttgcg cctgctgttt gacataggca gcttgttgtt gcgcgagacg
     2881 ctgcgcgcgt tcgttttggt aaaaatcgta attgccgccg tattgcgtga gtttttgctg
     2941 cgataattca atggtttggg tagttgccgc gttgagaaaa tcgcggtcgt gggaaatgat
     3001 gatttgcgtg cagggtaaag aggcaaggtg gttttccagc cacaagacgg tttctaaatc
     3061 caagtggttg gtcggttcgt caagcaagag caaatcggcg cggcaaatca gggcttgcgc
     3121 aagattcaag cgcatacgcc agccgccgga aaaggatttg acggggcggc tgtgttcttc
     3181 ttgcgaaaaa cccagcccgt tcaacaattt tgccgcgcgg gccggcgcgg tataagcgtc
     3241 gatttcttcc aatttggcat gatattccgc ctgcttcatg ccgtcatttt gcgcttccgc
     3301 ctgcctcaat gccgtctgaa aagcctgcaa ctcggcatcg ccctgcaaaa cgtaatccaa
     3361 agcggaaata tccaaatcgg gcgtttcttg ggaaacggaa gcgagccgcc agtttttcgg
     3421 aatcgagaca tcgccgccgt cctgagtgat tttacccttg attaaggcaa acaggctcga
     3481 tttgcccgtt ccgtttttgc cgatcaaacc gacgcgctga ccgggattga cggtagcgtt
     3541 ggctttgtcg agcaggactt tcaaaccgcg ttgcagggtg aggtttttga tttcaatcat
     3601 aacggaaaca tcgtcgggcg ggaaaagccc gtattttacc tgaaagtcag tgccgatgcc
     3661 gtctgaaacg ggaaatttac ggctgaagcc aagcccaagc cctgcgccct tccgagtgca
     3721 ggaaaaccaa tgtcctgaat gccgaatcgg tattcataca ttccacgccg attccgattc
     3781 gggaaaaatc cgccatgatt ttgggatgga taaactcctg agccgcgccc gtcccgataa
     3841 tcaatatttc cggatagtca tccgctttga catcagacaa cagattatcc ggagtcagtt
     3901 ctgacaatga ctgccatttc gagaggccgg cagaatccct atgcagcagc acaggcttat
     3961 tgaaatgtct gccggacagc cggatttcac ccgtgcggcg gtcatactct gcaaaatgtt
     4021 cgtctatcgg attttcttca aataacattt ttttatcctg ttgccgcttc atctgtatca
     4081 ggaggggtgc aaaatcagac aaattcatgt aggattagcg gatttcatct gacctctcct
     4141 gctgatttca gacggcattt gattcaaagt gcggcacaat tatatcggca gcggatattt
     4201 tcgtctttca atatttacat ttcagtcggc ttacaaggag acacaatgaa gccagtaaac
     4261 atcggtcttt taggtttggg tacggtcggc ggcggtacgg ctgccgtgtt gcgggacaac
     4321 gcggaggaaa tttcccgccg cttggggcgc gaagtgcgca tcagtgccgt atgcgacttg
     4381 agcgaagaaa aagcccgcca aatctgcccg tccgcagcct ttgtcaaaga tccgttcgaa
     4441 ctggtcgcgc gtaaagacgt cgatgtcgtc gtcgaattgt tcggcggcac gggcattgcc
     4501 aaagatgcgg tgttgaaagc cattgaaaac ggcaaacaca tcgttaccgc caacaaaaaa
     4561 ctgctcgccg aatacggcaa cgaaatcttc ccgctggcgg aagaaaaaaa tgtcatggtg
     4621 caatttgaag cggcagtagc gggcggtatc ccaatcatca aagccctgcg cgaaggtttg
     4681 gcggcaaaca ggattaaatc catcgccggc attattaacg gcaccagcaa cttcatcctc
     4741 tccgaaatgc gtgaaaaagg cagcgcgttt gccgatgtac tgaaagaagc gcaggcactg
     4801 ggctatgccg aagccgaccc gaccttcgac atcgaaggac atgacgcggg ccataaaatc
     4861 accatcatga gcgcgctggc attcggcacg ccgatgaact tttccgcctg ctacctcgaa
     4921 ggcatcagca aactcgacag ccgcgacatc aaatacgccg aagaacttgg ctaccgcatc
     4981 aaactgttgg gcatcacccg caaaaccggc aaaggcatcg agctgcgcgt ccaccctacc
     5041 ctgattcccg aaagccgcct cttggcaaac gtcaacggcg tgatgaacgc cgtgcgcgtc
     5101 aacgccgata tggttggcga aaccttatat tacggcgcgg gcgcgggcgc attgccgacc
     5161 gcttccgccg tggttgccga tatcatcgac atcgcccgcc tggttgaagc cgataccgcc
     5221 caccgcgtac cgcatctggc gttccaaccc gcgcaagtcc aagcgcaaac catcctgcct
     5281 atggacgaaa ttaccagcag ctactacctg cgcgtccaag ccaaagacga accgggcacg
     5341 ctggggcaaa tcgccgcgct gttggcacaa gaaaacgtgt ccatcgaagc actgattcaa
     5401 aaaggcgtga ttgatcagac cactgccgaa atcgtgattc tgacccacag cacggtcgaa
     5461 aaacacatca agtcggcaat cgcagccatc gaagcactgg attgcgtgga aaaaccgatt
     5521 accatgatcc gcatggaaag cctgcatgac tgagccgaaa cacgaaatgc cgacggaaga
     5581 gcaggttgcc gcgcgcaaaa aagcaaaagc caaaatccgc accatccgca tttgggcatg
     5641 ggtcattttg gcgttgctcg cttcaaccgc cctgctctcc caatgcgcga tgtccaaacc
     5701 gcaggcaaaa cagaaaattg tcgagtcttg cgtgaagaat attccgtttg ccgaaaaatg
     5761 gcaaaacgat ttacgggccc gcggtttaga ttcaaacaat acccgcctta ccgtcgacta
     5821 ctgcaaatgt atgtgggagc agcctttgga cagattgagc gagaaacaga ttagatcctt
     5881 cggcaaactc ggcgcacaag aacagcttga cctgctcggc ggcgcaaatg cctttgaagc
     5941 acgtgacaag cagtgtgttg ccgatttgaa atcagaataa tgtggaccga taaaaaagcc
     6001 gattctttaa agaatcggct tttttcataa aaaacggctt acagtgcgtc tttcaaagct
     6061 ttgccggcgc ggaatttagg cgttttggcg gcggcaatgg tcagaggctc gccggttttg
     6121 gggttgcggc cttggcgttc cgcacgttcg cccacgtaga aagtaccgaa accgaccaaa
     6181 gtaacggtgt cgccttgttt cagggcggtg gttactgcat tggtagtggc atccaaagct
     6241 ttttgtgcgg cggctttgga aatgtcggct tcttgagcaa tcgcttcgat caattcagac
     6301 ttgttcacaa tcagtccctt cctgtcttaa aaaatgatga aatgcccgaa tactcggggt
     6361 ttgtactgct tgagcaactt tcgctttata gcaattctga aattgccgtg tcaagcaaaa
     6421 aatacggaat caccctattt gacaggcttt caggacgaaa ccgcattttt acaacacatt
     6481 tcctgcgttt caatgtttgg ttgccctgct gcggggtttt ggttttgaag cggattccgc
     6541 cgccgcttcc gcaccagaag gttctgccca aggctcaggc tggctttcca aacccagagc
     6601 caatacctcg tctatccatt tgaccggatg gatggtcagg ccggttttca cgttttcagg
     6661 gatttcttcc aagtctttga ggttgtcttt tggaattaaa acgtgtttga tgccgccgcg
     6721 aagcgcagcc aacagttttt ccttcagacc gccaatcggc aggacttcgc cgcgcagggt
     6781 aatttcgccc gtcatcgcca catcggcgcg taccgggatt ttggtaaagg cagataccgc
     6841 cgccaaggtc atcgcaatac ccgcactagg gccgtctttc ggcgtcgcgc cttcgggaac
     6901 gtggatgtgg atgtcttttt tctcgtaaaa atcaggagcc aaacccactg attccgcacg
     6961 ggagcggaca accgaccacg ctgcggacac ggattccttc atcacatcgc ccaactggcc
     7021 ggtgcactga atcacgccct tacccggcaa tgctgcggct tcgacggtca gcaattcgcc
     7081 gccgacttcc gtccacgcca aaccggttac ttgtccgata cggttttcgc tttcggcaac
     7141 gccgtaatcg aagcggcgca cacccaaata gtcgtgcaga tttttctcat ttactttaac
     7201 cgccctgggc ttggcttttc cggttttttt ggtttttgag tcttcgtcca aggtaatctg
     7261 catcaccacc ttgcggcaga ttttggcaat ctcgcggtcg agcgaacgca cgcccgcctc
     7321 gcgggtgtag taacggataa tatcgcgcac cgcgctttct tcgattgcca attccccttc
     7381 ttttacgccg ttgcgcttca tttgcttcgg cacgaggtac tgcatcgcga tattgatttt
     7441 ttcgtcttcg gtatagccgg acagacggat gatttccata cggtcgagca gcggtgtcgg
     7501 gatattcagg ctgtttgaag tcgcaataaa catcacatcg ctcaaatcgt aatccacttc
     7561 ggcataatga tcggcgaact tgttgttttg ctcgggatca agcacttcga gcaacgcgct
     7621 ggcgggatcg ccccggaagt cgctacccaa tttgtcgatt tcgtcgagca ggaacaatgg
     7681 gttttttacg ccggcttttg ccatattctg caaaatctta ccgggcatgg agccgatata
     7741 ggtgcggcgg tgtccccgga tttcgctttc gtcgcgcacg ccgcccaaag ccatgcggac
     7801 atatttccgc cccgttgctt tggcgatgga ttcgcccaaa gaggttttgc ccacgcctgg
     7861 agggccgacc aggcacagaa tcgggccttt gagtttgtcc atacgttttt ggacggcgag
     7921 gtattccaaa atccgttctt tgactttttc caggccgtag tggtcggtat ccagcaccaa
     7981 tccggctttg gcgatgtctt tgctgacgcg ggattttttc ttccacggca gttcgagcag
     8041 ggtgtcgatg tagttgcgta cgacggtgga ttcggcagac atcggcggca tcattttgag
     8101 ttttttcagt tcggacaggc atttttcttc cgcttctttg ctcatccccg cctttttgat
     8161 gcctgcttcc aaggcatcca gttcgccgtt ttcgtcttct tcgcccaatt ctttgtgtat
     8221 cgctttaatc tgttcgttca gataatattc gcgctgggat ttttccattt ggcgtttgac
     8281 gcgtccgcgt atgcgttttt cggcctgcat aatgtcgagt tcggattcca gctgtgccag
     8341 caggaattcc atccgtttgc cgatttcggg aatttccaaa atctgttggc gttgcgccag
     8401 tttcaactgc aaatgcgctg cgaccgtatc ggttagccgg ctgttttcgg caatgccgtt
     8461 gatgctgccg ataatttcgg cggggatttt tttattgagt ttggcgtatt gttcaaactg
     8521 cgccaacagg gtgcggcgca cggcttcgag gtcggtattg ccgcccgtgt cttcttccac
     8581 gaccgcctct atatgggaaa cgaacagacc gcccgtgtct tcgatggtca gaacacgtcc
     8641 gcgatacaga ccttcgacca atacttttac cgtgccgtcc ggcagtttca atacctgtaa
     8701 gacttgtgcg acggtaccgg tctgatacag gtcggcggca accggttctt ctagcgccgc
     8761 accggtttgt gccaatagga aaaccggttc ctcgcgggta atggcgtttt ccagtgcggc
     8821 gatggatttc ggcctgccga caaacagcgg cagaaccata tgcgggtaaa cgacgacatc
     8881 ccgcaaagga agggttgcca aggcggcata ttcctcaaaa tgcttttctt tttgtgtcat
     8941 aggtactctc ttgtgtctga cagattgccg attttcgcgt acattggggt tgaaggtatt
     9001 atttcaagca tatgtggttt atttatggag tttgatgcga tgccgtctga aacattccgg
     9061 cttcagactg catgggcttg gaaagacaag gcgggaacaa aaaactgttc tgtgttgccg
     9121 ctccttgctg taccatccgt atggtttacg gttctgccgc cctattttca aaacatgacg
     9181 caggtatccc atgtcttctt ttatttttcc cgataacggg gccgaatggc gcaacgaaag
     9241 tgtgcaaaag ccgcccaaag gttggcatta tgtccgcgaa agcggtgcag acggcatttt
     9301 gaaggctgcc tatcaaaata ttgcaactgt ctggcagggc gatttccaca atgccaaaca
     9361 ggtgctttct gcaatgaaga agagggctcg caaacctgcc gccgtccgtt ccgatgcgga
     9421 tatcgccgcc cttttccatg cccaccgtat gaagcaggcg cagcagagcc gtattctgaa
     9481 tatgcttgcc gttgaaatcc gccccggttt tgtgttggac aacaaacgcg cgcccgatat
     9541 acgcgccgcc ctgctcgacg tgtacggaga ggcggacggc aaaccgtttt tcctgccgct
     9601 caatctgctg ctggggttta tgggtgcgca cgagtggcat aagaaagggg ttgccgttcc
     9661 gcagctgggc ggcagcatac acgttccttt cggcgtattc tcgccgttgc gcggcgaata
     9721 cctcgacctg ctcgcccatg cgccgtcaac gggttttcag acggcattcg atatcgggac
     9781 gggctcgggc gtgcttgccg ccattttggc gaaacagggc attccttccg tcatcggcac
     9841 ggataccaat ccgaaagccg tcgcctgcgc ccgtgccaat attgcccgtt tgggctttga
     9901 aaaacaggtt gagatacggg aaaccgatct gtttcccgaa gggtttgccg atctgattgt
     9961 ctgcaatcca ccctggcttc ccgccaagcc gacttccgcc gtcgaatccg cgttatacga
    10021 ccccgaatct gcgatgctgg ctgcgttttt gcgggatgcg ccgaaacatc tgaatcccga
    10081 cggagaaatc cgcctgatta tttccgacct tgcagagcat ttggggctgc gccctgccga
    10141 ttttttggaa aaggcattta tccgggcggg tttgcgtgtt gccgatatga tgaaaaccaa
    10201 gccgaagcac aaaaaagccg cgaatccgag cgatccgctt gcttttgcgc gaacccggga
    10261 aaccactttc ctataccgtt tgaaaaaggc ataaggggcg gcggcgcggc attcgggcgg
    10321 attattcttg tgaaaatacc cgctcgagca tactgcccaa tgccgtctga cgcgttttga
    10381 cagtggcggc agcttgccgg aaggtttctt ctccgccgaa gaatacgaac ttctccctcg
    10441 cgcgggtaat ggcggtatat aacagctcct tactcaatcc ggacaatgca tcgtcccctt
    10501 cgtccgaagg tgcggcggaa ggcggcagca gccagacttc ccgatattcc gaaccttggc
    10561 ttttgtggac ggtcatggcg aatgcgggtt cgaaatcggg caggcagctt accgctacct
    10621 ttttaaatcc gtccgcatcg gcaaaatagg cggcaaggct gccctgccgt ccgacatctt
    10681 ccataatcag tccgatgtcg ccgttgaaca gttcaagcgc gtagtcgttc tgcctgatca
    10741 taatcggctc tccggcgaaa taggccagat gttccggtat gttcattttg cggcgtacat
    10801 ggctgcaata ggctttgttg aagtcttccg catcctgccg ccaagctgcc aggaccacga
    10861 tatccgagat gcctgcgtat gcggcttcga tattgccgtc ttttaccgcc tgccaatagg
    10921 ctttgtgcgc ccggtacaac ctttcgactc gagcgttcgg actgcattcc gaatgttcca
    10981 gttcatccgg aaaccggtca aacaatgccc acgccccctc atcgcccgat acggcggcac
    11041 gggcaaggca gccgatgccg ctgttgtcgc cgaaacggtg gctgaacgac agatgggcgg
    11101 tgttttgcgc caacacgggc ggatttgcgc tgacgctgaa accgtgttcc ggaaggaagc
    11161 cggccagcct ttggtgcgct tctccgtcca aaacggtttt ttgcgacaaa acggacagca
    11221 ccgccccgat tccgacggac gggagctggt tttcatcccc cagcagaatc acgcgcgcgc
    11281 cggttttgac cgcttttaaa agttgcagca tcaatgccgt atccagcata gaggcttcat
    11341 cgataaccaa tacgtcaaac ggcagcgggt aaacagggtc gaacgccgcc tgcattttgg
    11401 gcgggcgcag cttcagcagt cggtggacgg tttgcccttc cagtttgagc aaatggcggc
    11461 ggacggcctc cggcgcgtca aaaccgttga ttgcacggtg cagcgcgcgc gccatatgtg
    11521 ccgccgcttt gcccgtcggt gcggcaagcg cgatgttggg aagattttcg tcttcaccgc
    11581 aaatcagcgc cagcagtttg gcaaccgtcg tcgttttacc cgtcccgggt ccgccggtaa
    11641 tcaccataaa aggctgcaac agtgccaagg cggcggcatc gcgctgccct tcgctgcccg
    11701 tgccttgaaa ccattttgcg aggttttgcc gcgcgcctgc cgcgtcgggg gcggacgtgt
    11761 cggctgccgc caagcgtttt atctcggcag ccaaatcgta ttccaactgc cacatcctgc
    11821 ccaaaaacag ccttctgcct tccaaaatca aaggcgcggc ggatgttccg accacgggtg
    11881 cgagtgccga cagcgcgtca gcctcgccac cgctcaaacg gataaacgaa tgaccgtttt
    11941 gcaatgcctg aaacaggcgt tcggtgcagt ttgcaagcac ttcgctgcct gaacccgcat
    12001 agcgtgccaa aaaccagatt gccgcccttg ccgccgcttg ggcaaattca tctgtctgcc
    12061 gttccattgt attccttgtc gaaatgccgt ctgaaggcgt ggggcttcag acggcgcggt
    12121 gttgtttcgg ctgtttaggc gtttgcgccc tgtttggggt gcaggctgcc gtctttcatg
    12181 accatcacgc gctcgaagcg gccggcgagt tcgtcgtcgt gcgttacgac caccagcccc
    12241 gttcccaatt ccgttttcag ttccagcatc atatccaaaa cattcctggc gttcgcacgg
    12301 tcgaggttgc cggtcggttc gtcggcaagc aggcattttg gttgcgtaac caaggcgcgc
    12361 gcaatggcgg cacgctggcg ttcgccgccg gacagttcgc ccgcgcggtg cgtcgaacgg
    12421 tgtttcagtc cgaccttttc cagcatcgcc attgccgttt ctgccgcctc ttcccgtttt
    12481 ttcttgccga tcagaagcgg catcatcacg ttttccagtg ccgaaaattc aggcagaaga
    12541 tggtggaact ggtacacgaa accgagatgg cggttgcgca ggatgcccag ttgtcgctgg
    12601 cttaatgtac gcaaatcctc gcccatcagc agcaccctgc cttcagacgg catatccagc
    12661 ccgcccagaa tatgcagcag cgtcgatttt ccgctgcccg aagaaccgat gatgccggtg
    12721 ctttcccccg cgcggatttc caaatccaag ccgtgcagca cccgaacgtc caaaccgccg
    12781 tcgcggtagc gtttgcccac gccttcgcat ttcaaaatca actcactcat aacgcaaagc
    12841 ctccgccggt tgggtttttg aggcacgcca gctcgggtag agcgtggcaa cgaaagacag
    12901 tcccaaggaa atgcaggcaa tcagggcaac gtcgcccata tcgacatcgc tgggcaggta
    12961 gtcgataaaa taaacctgcg aattgatgag gtggacaccg agcaggtttt caaaaaatgc
    13021 cacgactctg ccgacgttcc aacccaaaag cacgccgcag accacacccg ccagcgtgcc
    13081 gaaaaagcct gaaaacgcgc cctgcaccat aaaaatcttc atcacgccgc caggggaaag
    13141 gcccaaagtc cgtaaaatcg caatgtccgc ctgcttttcc gtaaccgcca tcaccaagga
    13201 agagacaagg ttgaacgccg ccacggcgat aatcagcgtc aggatgatga acatcatccg
    13261 tttttccaac tcgaccgcct caaaatagct tcgattgctg aacgtccaat cgcgcaccca
    13321 aaccgtatcc ctttgcgcct ccggaatcag tgttgccgtc aaggcggggg cgttttgcgg
    13381 gtcggcgagt ttcagccgca gccccgcaac ttccttatcc aagcggtaca gtacgcgcgc
    13441 gtcttggata tgcgtcatcg ccaatgagtt gtccacttcg taaacgcccg tcttaaccag
    13501 accgaccacg gtaaactgct tcaggcgcgg cacgactccg gcaggggtaa cgttgccctc
    13561 cggcgtgatg acggtaactt tattgccgac ttccgccccc aaagcctccg ccaagccgac
    13621 accgaggata atgtcaaact cgcccggaat caggtcttca aatttgcctg ccggcatttt
    13681 gtcgccatat tccaccactt tgcgttcttc agacggcaaa atgccgcgca tctgcacgcc
    13741 cctgatttcg cccgcgtttg ccagcagagc ctgattggaa acatagggcg cggcagccaa
    13801 aatacctttg cggttttccg taaaccgaag caggttgcgc caatccgtat ccgtattgtc
    13861 gatatagccg atttcggcgt gcggcgcgac attcaggagc tgcccgcgta tttctttctg
    13921 aaagccgttc ataaccgaca agacgacaat cagcgcggtt acgcccaagg cgattccggc
    13981 aatcgaaacc atcgtgataa acgacataaa gccgttgcgc tttttcgccc tgagatacct
    14041 caagcctatc caagcctcta gagaaaacat aacgctacct taaaaatgtc tgcaaacgtg
    14101 ccgccccgga cggcggtttg ggggcggcgg caaaagttta ttgtaccgta aaacccaggc
    14161 agcgtccgaa cgcccgagtg cgggggacgg ggcggcagag cgggcggaaa acttgcacaa
    14221 cgcgtcaaac tgccctatcc tttccttcag aaaaaccgtt tcttgaggaa aacaatgaat
    14281 atccgaactg cttttgcttt gtgcgccatc gccttatccg ccgctgcggc tgcctacgcc
    14341 aaagaaatca aaatcgatgc caacaacacg ccttattccg aagccgacgc gcaaaagctg
    14401 gcggcaacgg cagtcggtat gggtgttaag gaacctatca gcctgaacgg cggcagcggc
    14461 agcattaccg tgtccggcag cagcgcgacg cagtgcgtgt tcaaagtcgg caacggaggc
    14521 gcgttgcaga ttcaagggct aaactgcaag taaaccgccc ggaaaaatgc cgtctgaagc
    14581 cttcagacgg cattttgcat tggcggcgtt atgccccgcc ttctttaatc aggcggcgtt
    14641 cgtacaccgc ctgcgccagc gttcccgcat cgacatattc caattcgccg cccaagggaa
    14701 tgccctgcga cagcctgctg actttgtaag gcaggttttt aaaaaactcg gacaggacat
    14761 acgccgtcgc attgccttct gcggtaaaag cggttgcaat aatgatttct tcgacttccc
    14821 cgccgcccag ccgttgcgcc agcctgtcca atgcgatggc ggatacgtcc attcccaatg
    14881 ccgtattgat ttgccccatc aggacgaaat acagcccgtc gtggcagttt gccgcttcca
    14941 tattcgacac gtcggcaggc atatgcacca ccatcagccg ccgcccgtcg cgtgtttcat
    15001 cggcacaaat atcgcacaat ccgccttcgc aaaacgtgtt gcacatcgcg caatggtaaa
    15061 cctgcttcaa tgccgtctgc aaggcatcca ccagtttttc agcctctttg cgcttgtgtt
    15121 ggagcaaatg atacgctatc cgctgtgccg atttcggccc gacgttgggt aaaaccttca
    15181 gcgcgtcgat caatccttgg aaggcatctt gttttttgtg gctcatcata ttccgccgta
    15241 tgggaaaacg gccggaatat tccgaccgtt attttgtcaa caaaagtgtc aattactgac
    15301 cgtcgccgtt gtcgaccgat tgcgctcctt tggtctgttt gattttgccg ttgaaataac
    15361 gtatcaacaa gtcgaaagta ttggcagact gctgttgcgc caaagcctgt tttgcaagcg
    15421 gaagctgtgc ggcgatatca tccggcgggg ttacagcctg tacttcgaca atcacgggtg
    15481 ccggcagacc gatcagcctg acgtaggcgg gtttgccgtt tgccggtttt gctttcagca
    15541 gttccgcata agcctcgggc ggcatggact gccttgcctg ctgtgcgccc aaaacggaca
    15601 cttccgacca tttcacgtca acagccttgc cgccgttcag ttgggtaagc acgtcttttg
    15661 ccttgttttc ggcaagtttg gcggcttcgg tacggatata agcctgacgt accgcgtctt
    15721 tggcttcggc aaacggcagg gttttctctt cgcggacttc tttggcgcgg acgacccacg
    15781 cggtttcgct gttgatggtc agcacttctg aattgtgttt tttcttcaat acgtcgtcgc
    15841 tgaaaacggc attgatcagg ttttcgggca taccggacat ttgcgcatcc tgcctgctca
    15901 gccaagtttc ttgggtttcg actttcaaac cgctgttttt ggcggcttcg gcaagcgagg
    15961 aaggatggtt gaacgcgtca tcgcccagct tttcttttgc cttattgaag tcggcaaccg
    16021 cctttttcat tttcaattcg ttttctacgg cggctttttc ctgctcgaaa gaaggtttgg
    16081 cttcatttgc cggcaaacgc gccacgcgct cttcaaacgc atttttcact tctgtttcgc
    16141 tgacggtctg tttgtctgca aagtctttca gattcaaggc gacatattcc aatttgaccg
    16201 ctttgggaag caggtagtct tttttgtttg cgttataaaa cttctgcaaa tcggcttcag
    16261 acactttgac ttgggcgatg aattcgtcgg ggttgaaagt gtgcgaacgg atggtgcggt
    16321 tgacctgcgt cagcctgatc agctgttccg cctgcgcgtc gccgaccaat acgccgtttt
    16381 ggacgaggtt taccaaattc tgcaaggcaa actgatcgcg gatttcttcg acaaactggt
    16441 cttcagacat atgacgttgg gaaaggtagc ggtttaaaag cgcgtggtcg aatttgccgt
    16501 ttgcgtcgtg gaaattggga tcgtccacga taatctgctt gatttgttcg gaagaaaccg
    16561 aaatgcccat cagcttcgcg ccctgtttca ggtaggcgcg ttgcagcagg gattggaaca
    16621 ccgcgtcgcg cgaagggctg ccgccgtccg cctgttcatt ttgcatagcg ttgttgatgg
    16681 agtggtcgct gattttttcg tcgcccactt ggacgatgta gtcggcaccc ggatgggata
    16741 ccgtgctgac cccgaagccg acgaaggtta atgcaatcag ccccaaaagg acttgggcgg
    16801 gcgttctgta tttttcgatg gaatggaaca tattttcaga ttggaaatgg gattgggaaa
    16861 ttcaagtcgg gtattgtaac ggtttttatc cctgtctgca cagggcttgc cgattgaaga
    16921 tgccgtcgta ggtttcttcg ctgaagccga cataaaccct gccgccgcac tccaatacgg
    16981 gacgcttgat caggctcggc atttcggaca tcagtttgac ggcctccgcc gtcgaggaca
    17041 gcactttttg ctgtgttccg gcatcaagtt tgcgccagct cgtcccgcgt ttgttgagca
    17101 gggttgccaa aggcacttgt tccagccacg agcagatttc cgcttcagac ggcctctgtt
    17161 ttttaaaatc ccgaaattca aactccaagc cgtatccggc aagcctgttt ttggcttttt
    17221 tgactgtgtc gcaatttgga atgccgtgaa ggattatcat ttggaaacct tttgtctgaa
    17281 ataataaaac ggatatttta ctataagtgt ctgaaaattt gcccgtctgt ttcagacagc
    17341 ggggcggtta tgttacaatc cgaaaattcg aaaaatttaa tctcttgttc aataaaggct
    17401 ttaccaatca tgatttctac caacggcatc accatgcagt tcggcgcgaa gccgctgttt
    17461 gaaaacgtat ccgtaaaatt cggagaaggc aaccgttacg gcctgatcgg cgcgaacggt
    17521 tcaggcaaat ccaccttcat gaaaatcctc ggcggcgatt tggaacagac ggccggcgaa
    17581 gtggcgattg aaaacggcgt gcgtttgggt aaattgcgcc aagaccagtt tgcctacgaa
    17641 gacatgcgcg tgctggacgt ggtgatgatg gggcataccg aaatgtgggc ggcgatgacc
    17701 gaacgtgatg cgatttacgc caatcccgaa gccaccgaag acgactacat gaaagccgcc
    17761 gaactggaag ccaagttcgc cgaatacgac ggctacaccg ccgaagcgcg tgccgccgaa
    17821 ctgttgagcg gcgtgggcat ttccgaagat ttgcacaatg cgaaaatggc ggaagtcgcc
    17881 ccgggcttca aactgcgcgt attgctggcg caggcgctgt tctccaagcc ggatgtattg
    17941 ctcttggacg aaccgaccaa taacttggac attaatacca tccgctggtt ggaaggcgtg
    18001 ttgaaccaat acgactccac gatgattatc atcagccacg accgccactt tttgaacgaa
    18061 gtctgcacac acatggcgga tttggactac aacaccatca ccatctatcc gggcaactac
    18121 gacgactaca tgctcgcttc tgcccaatcg cgtgagcgcg ccctgaaaga caatgccaag
    18181 gcgaaagaaa aattgcagga attgcaagaa tttgtggcgc ggttcagtgc caacaaatcc
    18241 aaagcccgtc aggcaaccag tcgtctgaag caggcagaca aaatcaaatc ggaaatggtc
    18301 gaagtcaaac cctctacccg tcaaaacccg tatatccgtt ttgaagccga tgaaaaagcc
    18361 aagctgcacc gtcaggctgt ggaagttgaa aaactggcga aacgctttga aacccagttg
    18421 tttaaaaacc tgaacttcat ccttgaagcg ggacaacgcc tcgccatcat cggcccgaac
    18481 ggcgcgggca aatccaccct gctgaaactc ttggccggcg cgtacaaccc cgaatattca
    18541 gacggtctgc tgccggacga aggcagcatc aaatgggcgg aaaaagccag cgtcggctac
    18601 tatccgcaag atcacgaaaa cgacttcgat gtcgatatgg atttgagcga atggatgcgc
    18661 caatgggggc aggacggcga cgacgaacaa gtcatccgcg gcactttggg gcgtttgctc
    18721 ttcggcagta acgatgtcgt gaaaaaagtg aaggttctct ccggcggcga aaaaggccgt
    18781 atgctttacg gcaaactgtt gctgttgaaa cccaatgtct tagtcatgga cgaaccgacc
    18841 aaccacatgg atatggaaag catcgaatcc ttgaacatgg cgctggaaaa atacaacggc
    18901 acgctgattt tcgtctccca cgaccgtcag ttcgtttctt ccctggcaac ccaaatcatc
    18961 gaactggacg gcaaaggcgg atatgaacac tacttgggcg attacgaaag ttacttggag
    19021 aaaaaaggcg tagcataacc gccggttgga acaatgccgt ctgaagccgc ttcagacggc
    19081 attgttgata actttaaaat aggaagcata tgcagactta cctcgtcggc ggcgccgtcc
    19141 gcgattatct tttgggcttg cccgtcaaag accgcgattg ggtggtcgtc ggcgcagacg
    19201 cacaaaccat gctggcgcaa ggcttccagc cggtcggcaa agattttccc gtgtttctcc
    19261 atcccgaaac gcacgaagaa tacaccctcg cccgcaccga gcgcaaaacc gccaaaggtt
    19321 acgtcggttt cagtttccac gccgacaaag acgttacgct ggagcaggac ttgatgcgcc
    19381 gcgacctgac catcaacgcg atggcgcaag atgcggacgg caagattatc gaccctttcg
    19441 gcggacaacg ggatttggcg gcaggcattt tgcgccacgt ttccccggcc ttcgccgaag
    19501 accccgtccg catcctgcgt actgcccgct ttgccgcgcg ttacaggttt gaaatcgccg
    19561 aagaaaccat aaagctgatg cggcagatgg tggaaaacgg cgaagcggac gcattggttg
    19621 ccgaacgcgt ctggcaggag tttgcgaaag gtttgatgga aaaaaatccg cgcaaaatga
    19681 ttgaagtgtt gcgcgaatgc ggcgcgctca aagtcttgct gcccgaagtc aatgccctct
    19741 tcggcgtgcc gcaacgcgcc gactaccatc ccgaaatcga cagcggcatc cataccctga
    19801 tgacgctgca acgcgccgcc gatatgggtt tgagcctgcc cgaacgctat gccgccctgc
    19861 tgcacgactt gggcaaagcc aaaacaccgt ccgacatcct gccgcgccac cacggacacg
    19921 acctcgccgg tgtcgaaccc gtgcgcgaag tcaatcagcg gctgcgtgcg ccgaaacatt
    19981 gcgccgagct tgccgaattg gtttgccgtt ggcacattat tttccaccaa gtcggacagc
    20041 ttaaaagcca aaccattctg aacgtattga aaaaaaccga tgccttccgc cgtcccgaac
    20101 gctttcagac ggcattgaac gtctgcattg ccgacacgca aggccgtctg aaccgcgaac
    20161 acacgcccta cccgcaacgc gcacactggt tcgccctgct cgaagccgcc aatcaggcgg
    20221 attcgggcaa aatcgccgcc gaatgccgcg cgcagggaaa agcgcacctt atcgccgaac
    20281 aaatcgaccg ggcgcggctg gcacaaatcg ccccattgca aaaaacgttt cgagcggcgc
    20341 aagacaaaac agaaaaacat taaaacgtcc aatgcagcca ctttta
//
LOCUS       DAWEWP010000023        19820 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWEWP010000023 DAWEWP010000000
VERSION     DAWEWP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19820
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.023"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(232..307)
                     /locus_tag="WGN53_000598"
     tRNA            complement(232..307)
                     /locus_tag="WGN53_000598"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Asn,seq:gtt)
     gene            complement(400..759)
                     /locus_tag="WGN53_000599"
     CDS             complement(400..759)
                     /locus_tag="WGN53_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ0139617.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(782..1426)
                     /locus_tag="WGN53_000600"
     CDS             complement(782..1426)
                     /locus_tag="WGN53_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ0139618.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1428..1964)
                     /locus_tag="WGN53_000601"
     CDS             complement(1428..1964)
                     /locus_tag="WGN53_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ0139619.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1976..3073)
                     /locus_tag="WGN53_000602"
     CDS             complement(1976..3073)
                     /locus_tag="WGN53_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0139620.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLASVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3076..4512)
                     /locus_tag="WGN53_000603"
     CDS             complement(3076..4512)
                     /locus_tag="WGN53_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ0139621.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4732..5514)
                     /gene="bioC"
                     /locus_tag="WGN53_000604"
     CDS             complement(4732..5514)
                     /gene="bioC"
                     /locus_tag="WGN53_000604"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ0139622.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5502..6149)
                     /locus_tag="WGN53_000605"
     CDS             complement(5502..6149)
                     /locus_tag="WGN53_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ0139623.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKVSFEDYQQFPARPFDEIHQELTALFEMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6159..7301)
                     /gene="bioF"
                     /locus_tag="WGN53_000606"
     CDS             complement(6159..7301)
                     /gene="bioF"
                     /locus_tag="WGN53_000606"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ0139624.1"
                     /translation="MNVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEQLVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAVCL"
     gene            7524..8600
                     /locus_tag="WGN53_000607"
     CDS             7524..8600
                     /locus_tag="WGN53_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ0139625.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8807..10273)
                     /locus_tag="WGN53_000608"
     CDS             complement(8807..10273)
                     /locus_tag="WGN53_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ0139626.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10471..11394)
                     /gene="speB"
                     /locus_tag="WGN53_000609"
     CDS             complement(10471..11394)
                     /gene="speB"
                     /locus_tag="WGN53_000609"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ0139627.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11493..13385)
                     /gene="speA"
                     /locus_tag="WGN53_000610"
     CDS             complement(11493..13385)
                     /gene="speA"
                     /locus_tag="WGN53_000610"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ0139628.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYASTVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13552..13836)
                     /locus_tag="WGN53_000611"
     CDS             complement(13552..13836)
                     /locus_tag="WGN53_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0139629.1"
                     /translation="MKKLLLAAVVSLSAATAFASDSAERQIYSDPNFEQNRVKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13987..15795)
                     /gene="aspS"
                     /locus_tag="WGN53_000612"
     CDS             complement(13987..15795)
                     /gene="aspS"
                     /locus_tag="WGN53_000612"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ0139630.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYAEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAE
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(15853..16554)
                     /locus_tag="WGN53_000613"
     CDS             complement(15853..16554)
                     /locus_tag="WGN53_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ0139631.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16697..17824)
                     /locus_tag="WGN53_000614"
                     /pseudo
     CDS             complement(16697..17824)
                     /locus_tag="WGN53_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236077.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
     gene            complement(18005..18268)
                     /gene="rpsT"
                     /locus_tag="WGN53_000615"
     CDS             complement(18005..18268)
                     /gene="rpsT"
                     /locus_tag="WGN53_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ0139632.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18571..19713
                     /locus_tag="WGN53_000616"
     CDS             18571..19713
                     /locus_tag="WGN53_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0139633.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5107 a         5109 c         4596 g         5008 t
ORIGIN      
        1 agaatcgttc tctttgagct aaggcgagcc aatgccgtac tggtttttgt taatccacta
       61 taaaatcgag tttatcctag ttgtccagga cagcccctat tttttcataa ccatcacgaa
      121 aggaattttg caaagatttt attccatctc aaaacaatca tctcaaaaat gcgtttctga
      181 ccgccggtaa aacaaaaccc tctaagaaaa tacttagagg gttttgaaat ttggctcccc
      241 gacctgggct cgaaccaggg acctgcggat taacagtccg tcgctctacc gactgagcta
      301 tcggggaata agcgcgaata ataaaacaga aataccgaac gtgtcaatat aaattgacaa
      361 tcgattgcac tttatctgca agcccgcaat ctttatatgt tatatggctt ccgcaatatg
      421 ccttgccgca cgtttggcat ccaataggat taaaccaagt ctgccggttt cttttgccac
      481 cagcaccaac acggcatctt tacccgcctg acttaaaagg atatagcctg attttccttt
      541 aatcatcact tgttccaatt ccccgcaggc gagttcctgc accgagcgac tccccaaagc
      601 aagcaaagtg gcagaaatcg cccctaccct gtccgaattc aaatgtgaag gaagcattgt
      661 cgccatcggc aatccgtcgg ttgagataac ggcagacgcg ataatatccg tagatgtatt
      721 gtttaaatct tcaaggattg aaatcaataa ttgctgcata acccctcctc tctcccaagt
      781 tttacacgcg gttgctgtaa cggcggtata aaatccttac caaagtaaca aatgcctctt
      841 tgcccaaatc gggaatgccg ccgataacca aaataaattt ggttgaaccg atatacaatg
      901 ggaaaaatgt caattcgctc tgaccggaag gatcgcaaac gccccaagcg ttattgttga
      961 tatacaggtt gttcttaatc agcagccggt atttcttttc catctgtgcg acttctgccg
     1021 ccaacaaccc caactcttcc gccgcctcat gatggaaatt ggcgttggca agatacagac
     1081 cgttccgatc gaccaataac gccttaccgc tgccggacaa ttgttccatc agcaacggca
     1141 attgctcgtc cgacaaattg atgccgtctg aatgaccgtt ttcatcgcca tagaggaatt
     1201 cgagtttttg caaacggtac aacaggttca aagcggtatc gatgtcggcg gtgtccgccc
     1261 aggtaagcag cttctcactg ctgaccattt cgtccgcatc cgctttcaac aggctgtgca
     1321 acaaagtttt accggcactg ggggcatcgc tggatacggc ataaaatgca ccggcaggag
     1381 tcaggcgggg atataaattt gcttgtagtg aaagtgttga ttccatatta aacctccagt
     1441 cccggatcaa tagaaaataa cattgcgcta accaattgtt ttacgtcatc ttccttacgg
     1501 gcatcaattt caaaaaccgg aacattaaga ttatgttttg caagatattt gtgatacacg
     1561 tcgataccgg gctgagagcg tatatccatc ttggtaatac cgacaacgac gggtgccttc
     1621 tccagcagcc ctcgaaacga atgtaaaaag aattccaaat ctttcaacgg attggttcgg
     1681 gcattatcta aaagcaagac caaacccata ctgccttggc ttaagatttc ccacataaag
     1741 ttgaaccgtt cctgaccggg cgtaccatat aaatggactt tggtatcctc atccaagctg
     1801 atggccccgt agtccatcgc cactgtcgta ttccttttcc tatccaaagt catatcggat
     1861 gcggaagcat cggtctgaac gagtgcttcg tccgaaatag ccgcaatggc agtggttttc
     1921 cctacgccga caggtcctgt gaaaataatt ttattttctc tcatctctcc gcctcttagc
     1981 tgcccaacag ttttttcatc agcctttgca gaaggccgcg cgactgggat tgggatggtg
     2041 ttgtgatttt ttccgctttt ttcatcattt cactatcaga ggcagaatcg gctccgatat
     2101 ttattttatc cgccatatcg gagtatgcct gttgtgaaac cgtttttaaa tctaccgaca
     2161 aaaacccggt tgtataggtt gccgcaagat aattcagaat atcattgagg tttaaaggca
     2221 tcactttata caacacgtta aggttgacgg atgccttggt cagaaatgcc gacaagcgta
     2281 tcgaccccgg cacacttgcc aaccgggtca ggtttggcca agatttcaac gtaaacggag
     2341 tatcgggaga aatcggataa atcaacctgc cctgcgccgt ccaaatggaa aactgccaca
     2401 tacaggacat aatgcctact ttagcctttt cgcgccattg cgggttatcg ggaacagtct
     2461 tgcagctgac ctgcaaattt tcgtctttgc acaattcttc gagtttttgc acactttctg
     2521 tcagcaaaac ccgttgtatc gaggggaaaa caataaggac cggcttattt ccatgcaaga
     2581 tagcgatgtc ctgcctgttc ttttccgcaa accgcaacgc ccccaataat cctttattcg
     2641 ggttaaactg gcgtatcgta accgtacgct gcacattccc gttatttttc gccgaccggt
     2701 ctgcgggtgc aataaacgat ttcccataaa cattctcgcc ctgcaacaat ttgcggagca
     2761 taggaaacaa tgtttcaaac cgaatcggtt tgggcaggta gggaacttca gaatcgggaa
     2821 ctttctccga acagacggcg acgggtatat ccttataacg ctcggcaagc tctttccaaa
     2881 gttcaaaacc gccctcggca tcggtatccg ccaaaaccaa atcgggcacg gcactgccgt
     2941 ctgaagggga tactgtttca taacgggtgg tattgtgcat tttgaatgcc attttgaaaa
     3001 cggattcctg ctgcgccgtc atccccgcca acattacgcg tactgtttta attttcggca
     3061 gttgaacttc catttttatt ttccgtaccg ttttattttt ttaatatttt gattcatacg
     3121 ctgcagcagc cggctcatca gcataacgac ctcttcagga agcctgtccg cacgttccct
     3181 caataccctt aaaaactgcc ccaacctatc ccaatcttca gtacgttcat aaatatcgat
     3241 caacgtaata taaagctggg actcgtcggg atatttcaat accgcctgct ccaacacatc
     3301 cattgccgct tcaatctgac catacatcag caacgactct acttctttaa ccgcatcgtc
     3361 tgccggagac gaaccggtgt tgatcaacga agaatcttga agcaccaaat cccgatgttg
     3421 cgatttgaat ttctgtatat ttttcggcag atacccgtgc cccataccga tgtctttgat
     3481 ttgccggtcg ttcggccctt tttccaaatc atcgaaaact tcatggtaac ccaagctgta
     3541 cccccacccc agcatccgct ctttaacctg cctgccgtag ttgcccaacg tttggtaaag
     3601 tttccacaaa tgtccggcaa aacggtctat gtccgcgtgt tggtaatcga gtttcaacgc
     3661 atcgataatc aggtttgcag gtttttcgga agtttggatg gcacggtcgt attgtttcga
     3721 tgccgtttca tagctgactt tgtctttaag gattttcgca ccttgatcgg cacggaccaa
     3781 accggcaatc gcaccgattt cctcttgact gattccggac acgtcttttt tgccccgcac
     3841 aatcgggatg cgcttgattt cttcggcttc ataagcctta ccgccggcat ccggcggggg
     3901 cgatgcggaa gctgccgtcg cagaaccgcc ttccgcacgt ttttcaatct cctgagtacc
     3961 ccatcccaaa ccttcttccg ccaagacgcg gatacgcaaa tggtttgaat cgcgctgtaa
     4021 tgcctgttcg atatattttg ccaaaagttc ggaaggaatc agtttgccgt atttttgcag
     4081 attgtctgcc aaaacatcga catcccccac ttcgagattg atatcgagca gctcgcggat
     4141 aaggttttca ggtttcgctt caccatccgg aatgccgtcc agataggcag ccaaagattc
     4201 ggcagccttg ccctgataac cgaattgctt ataaacctga tactccgtaa gcggatcgac
     4261 ttcttgcgcg gatacggcgg cggatgcggt ctcggcactt tcgttccaag accagtcggg
     4321 ttgcgtgccg tctgaaaccg attgtccaat ctgatcgacc cagtccgaat cattgcttcc
     4381 gggtgtctgc ccctgcgtat tgccgacacc ttgggatttg cgcttcggtg ttttcttacc
     4441 ctgcttcgca cgcataacca aaagcatcag caataccgcc agtgccaaac cgataattaa
     4501 tgagttttcc aaaggatact cccgataccg taacgagcgg atagctgacc gcctgcaacg
     4561 gccattgtcc gcttttatta ttaacgattt actgaaatag acttgtaagt tttaaatcat
     4621 accataattt aacgtttaac aatatgcctt ctgcacaagc ctcgccatat taccttttac
     4681 ccacatcagt atcaataccc gatataaaaa taactttgcc cataagctgc cttattgcct
     4741 gcccgccgca gagtagcgcg cgataaaaaa taacggatga taagtcaggt gcactttgcc
     4801 cgacggcata ctgaacgccg acaaataatc gccaataaat ccattgagat tttttcttgt
     4861 ccaatttttt tggttcgtgg ctgttacgcc tgtatattta aggtgtttga gtacatctaa
     4921 cggcgtatca aagtctagta ttaccttaaa atcctcacac cataaaagct caaaatcttt
     4981 ggctaaccaa gtctgccatt gggataaagt cgggtaattt aagcctatat ttgtaatttg
     5041 gcggacttct tttaaattgt ctttgccaaa ggttgcaacc gccaataatc cgtttgcttt
     5101 caagcctgtt ttgcaatggg cgataaaagc gtcgggttga tgaaaccatt gcacggcaga
     5161 tgcgcttgcg attaagtcaa attgtcgttg aaaaggaaag ttttccgcat cgccgcaata
     5221 aaaatcaaag gattgcggca gtttttcagc cagttggggc tgcacatcgc acaaatcatt
     5281 aaataaccaa taattcgctg aaatctgttt ttgcagcaag gcactcaaca ttcctgagcc
     5341 gcagcccaat tccaacacgt tttccaatgg catatccggc aaataatctt gcaaatgcgc
     5401 cattaaatta atcgtcattt tttgttggat taaggcgtgc cggtcataat cgtttaatgc
     5461 tttttggaaa gcctgccgaa tgcgcgattt atttatggca gtcagtgatt ccatagtgcc
     5521 gaccaatggg tgaatcttga aaacaggtaa tgtccgccgt caatttcccg aacggtgcaa
     5581 cgcggtgtcc aatatcggtg ctgattggca ggcataaaaa ttttatcgcc tgatccgacc
     5641 aaggcatttg tccagcgaat caaatctgcg cgcctatctt gcccgatcat ctcaaaaagt
     5701 gcggtaagtt cttgatgaat ttcgtcaaac gggcgtgcgg gaaattgttg gtaatcttca
     5761 aaagatactt tatcgccaca cattctgcgt tcaaatttta aacgggtgtt ttccgtgagg
     5821 ttctccaatg tgcctttaaa aacggcgcaa gggataccga aattatcatc gcaaggcaaa
     5881 cctgtgccat tcactgccgt tgcggatttt aatcttattc cttgcaatgc cctctctgcc
     5941 gcccaaacgc ccattgacca cgccaccaaa cggatgtgcc gataggcgga aaaatcaaaa
     6001 tccaaattta aatcttgata atcgtagcaa atcaataaat cgtgattttc cggcaaaatc
     6061 aaatgattta cagcatcggg cggcgttccc caacctgcaa aatacaggat taaatgtccg
     6121 ccttgatgat tgtaaaattt tgttttcata tcatatcctt acaggcacac cgcaaactgc
     6181 cgcacttcat ccattgtcat atctgccgtc aaagacaatc gtattctgga tgtacctttt
     6241 ggtactgtcg gtggtctgat gggcaggcaa taataaccct gcctttgcag gtattccgct
     6301 ttggcaaggg tggcttcatt cccgcctaaa atatagggga cgatacaggt ttggctcggc
     6361 attatttgcg tccgatgcgc cacttcccgc cgtaaaaatg cgcttaactg ctcaagatgg
     6421 cttctttctt ttgagaattg cggcaatcgt tcaaaaataa aataagtcca agccacatta
     6481 aacggcggca atgcggttga aaaaatcaat gggcgcattt gattaatcaa acattctttc
     6541 aatacttggt tgcagacggc atacgccccc accgaggcta aggctttacc gaaagtgccg
     6601 accaataaat caatctcggc aatcaaatta tcccgttcgg caatccccaa tccgttttgc
     6661 ccataaacac cgattgcgtg ggcttcatcc acataaagat aagtattggg aaactgtttt
     6721 tttaattgga caagctgttt caaatccgcc acatcgccgt ccatactgaa aacagattcg
     6781 gtaacgataa aagtgcggtc aaattttccg acgttttttt caagcagatt tttcaaatgt
     6841 tcataatcat tatgacgata acggaaaaac gcacaccggc tcaaacggat gccgtcaatc
     6901 atactggcgt gaacaaattt atctgccaaa atcaaacttt tcgtcgtcgt caaagcaggc
     6961 aaaataccga gattggcgtg atagccgctg ttgaacaata acgcgctttc ccgttggaaa
     7021 cgttgtgcga caagctgctc aagatcggta taaataggaa agttgcccgt taataaacgc
     7081 gatgaagaac tggtaaaaga gggaaaatta ccgccgtatt gctgcaaaaa agaccggcgc
     7141 aagttttcat ctgatgccaa acccaaataa tcattagacg acatattcag cattttgcgg
     7201 ttttcccgcg taatataccg cccttgatga atcaaatccg gaatcgaacg atattggttt
     7261 tgcgcgccga gttgttcaag ctgttgttta aaaacattca ttatgatgta aatattcctg
     7321 agttaaagcc tgaaccaacc cagcatcaaa caatcaagta atgccctttt aataaaaacc
     7381 gttttgccgc cgacctttgc cacacccctg ctttcataat ccaaggcgga aatttccgcg
     7441 acatttgttt ccgttgccat tacccaatcc gtccgcactg taccgaagcg catattttcg
     7501 cacaaattgg tacggtttca ggtatgattt gccgaaaacc tatcggacac acaccatatt
     7561 ttgatatgaa taaaacaatc tgccgcactg ctgcactatt gataagcggt ttttcatacg
     7621 caaacaccgt cattcccgat gtctcccccg tcgcacaagg tcaacatgtc ttcattaaca
     7681 tccctcagca acgcctgttc ctctacaccg acggcaaact gaccaaggtt tatcccgttg
     7741 cagtaggtcg ggcgatgacg caaaccaatc taggcgaaca taaaatcggt gcgaaagcct
     7801 ataaccctgt ttggtacatc cccaaatcca tacagaaaga acgcggcgac ggcgtaaaaa
     7861 ccatagccgc cggcccggac aaccctttgg ggccggtttt tgtccgctta ggcgacccca
     7921 aactcggttt gggcatacac gggaccaatg cgccggccag cgtccccggg gttcggagtc
     7981 acggctgcgt ccgcatgaaa tcgcccgacg cgctcgagtt tgccaaaacc atcgccagcg
     8041 gctcgcccgc ctccgtcatc tatcaaatgg cgggtctcaa tgaagatgcg gatcgcaacc
     8101 tgtggcttgc cgccttccgc gacccttacg gtaagaacaa ccttgacatc gcctctctga
     8161 aaaaaagtat tgcgcaatgg gcaaaaacac agggtaaaac catcgcgccc gagaaagtcg
     8221 atgctgtact caaagaccgc accggatcgg ccgtctgcct gacctgcggc aaaaacggca
     8281 agatgaagat gccgctcaaa tcgctggcgt ggatacaggg ttcttcctca tacagccaag
     8341 ctgaagtcat tgaacaaact gaggaaacca actctgccga aatatccgag acacgcacgc
     8401 ccgaagtgcc tgatgtacac acgcccgagg cacaaccgca tttaaatacc caatccgacg
     8461 gcacgccgac tgcctatacc gaaccggctg ccgattcatc gccgcaagta gaaacacctg
     8521 atcaggctgc ttccgagccg gttgatgtat tattttcaat agatgtgata cggcagggaa
     8581 atttgcgttt aggtaattga ataaccttct gattatttaa tgcttatgtt tatcagaagt
     8641 tgataggcgg tttggttttt gtttggcttt tcctacaccg ccgcctgccg ctttctgcaa
     8701 cattcaagcg cacaaatatg ccatctgaag gctttagacg gcatatttca cgataatagt
     8761 cgcccaatca aataaacaca gattggaaat ttattttatt tttcccttac caatacccca
     8821 gaactttcca ccaaatgctg ccgataacga aaaaaatcag aaaattgact acgctcatga
     8881 taaaacccgc cttccaccat tctcccattg tggtgtagcc cgaaccgaaa atcacaggcg
     8941 aagtaccggt cgcataatga gtgagggtca tcataatgtt agatgcggcc gccatcatca
     9001 gcgcggtcgg catcgccggg gcattcagtg aaacggcagc agcgaaaaat gcgccgaaca
     9061 tagcggtaat atgtgcagta gtactggcaa acatataatg cgcatacata taagcaagca
     9121 cgaggattac gcccgcagcc gtgccgctaa cgcccaaacc gccgacactt tccgccaaca
     9181 ctccggagaa ccatttaatc agtccgagtt tatttaaaaa tgcggccatc ataatcaatg
     9241 cgccaaacca aataatcgta tcccacgcac ttttttcttt caaaacatcg tcccaagtca
     9301 atacaccgga aagcaaaagc aggcttaatc cgataaatgc ggtggcggtg gcgttgatac
     9361 taaaagcgtg attgccggta ataagggcgg gaacatctgc ccacaacagc agcaagatac
     9421 cgaaaatgac cgccataatg atttcgtctg ccgacatttt acccatctcc ctcagacggt
     9481 ctttggcaaa ttgaacggca ttgggcgttt ctttaatttc aggcggatac aaaaaatata
     9541 aaatcaaagg cataacgaaa aaggcgataa cgccgggaac agccattgcc cacgcccacg
     9601 ccccccaaga aagacggaaa ctactgccta aattttcggc aatcaagttg acgattaaag
     9661 ggttgggggc agttgcagta ataaacatag ccgacgaaat gggattggaa tgatagttga
     9721 ccaaagccaa atatttaccc atcttgcctt ctgtgccttt tgcgggattg gagccgtaac
     9781 tgccggcaat cgactgcata atcggatgta taatgccgcc gccgcgcgcg gtattggaag
     9841 gggtaacggg agccagcagc agttcggaaa gagcgagact gtaaccgatg cccagcgttt
     9901 ttcttccaaa aacggcgata aacaaatatc cgatacgcat ccccagccct gttttgagca
     9961 aaccgcgcga aatcataact gcgatggcaa tcagccaaat caacggattg gcgaacgcac
    10021 tcaacgcatc gctcatcgcc gcgcccggtt tgtcggcggt tacgccggtt actgcgacca
    10081 acccgacggc aataatcgac agcgcgccca acggcatggc cttgccgata atggcggcaa
    10141 tcacaccgac aaacatggcc agcagcgtcc aagcctgagg cttgaccccg tcgggtacgg
    10201 gcagtgccaa aaccagggcg cacaatactg cggcaatggc gaggggtatc ggtttgaaac
    10261 ccaatttcat catattgacc tccgtaaaaa agaccgtccc gaaaaatcgg aaaaataata
    10321 tttaactaat tgttttataa tatatattca gatatttcac cgtctttccg atatgcggct
    10381 ccgggcaact tttgtttcag tatttgaatt ttcattagac tgaatacgcc gtttgaacgg
    10441 cacggcgaaa acccggggga tgccggacgt tcagtccttt ttcgcacctt gaaggtaaag
    10501 catttccaag gcaattgtgg cgccggccaa agcggtaatg tcggattggt cgtaagaggg
    10561 ggcaacttct acaacatcca taccgacgat gtcgagatcc gtcagcccac gtaggatttt
    10621 taatgccctg tcgctgctca agccgccgca tacgggcgta ccggtcccgg gggcgaacga
    10681 cgggtcgagg cagtctatgt cgaaagtcag gtaaacgggc atattgccga cggtttcttt
    10741 gattttacgg acggtctctt caacactgtc ttcattaact tggggggcgg tcaacacagt
    10801 aaaaggcaat tttttactgt gttcggtacg tatgccgatt tgtacggaac gggacgggtc
    10861 gatgaggcct tccttggggg cggtatagaa catcgtaccg tggtcgtatt cgctgccgtt
    10921 gtcgtaggtg tcggtgtgcg cgtcaaaatg aatcagtgcg agtttgccga aatagcgggc
    10981 gtgggcgcgc aacaacggga gggtaatgaa atggtcgccg cccaaactca aacagcgttt
    11041 gccggaagaa agtaatttgc cggcgtgcgc ttccattttt tcgacaaaat ccctgctgtc
    11101 gccaaaagaa aaaaccaagt cgccgcaatc aataatgttc aggcgttcgc gcacatcaaa
    11161 tgtccatgga aacctgcggt gctcccaagc gaggttgacg gaggcgcgcc ggatggcttc
    11221 aggaccgaaa cgcgcgccgg aacgccctga aaccgccata tcataaggca cgccggtaat
    11281 aacccaatcg gcatgacttt catacggcat aaaattaagc ggcaggcgca aaaacccgaa
    11341 attattggaa acgagggagt tgtcggtttg tcctgccagt gtgctgtatt gcatcgtaat
    11401 gattccttgt aattggtttc aatcggtcgt gatgattggt gtttgagtaa gaaaatcggg
    11461 cttcagacga catatccgat gccttgatgc gtctattcgt cttccaaata ggtataacca
    11521 ttaagccccg cttcgagttc ttttaagaaa gacatagcct gcgaggcagg aaggtctgaa
    11581 tgttcgattt gttcgcgata gcgtttcatc agctctttcg gatcttgata aacgtattcg
    11641 agcatatcgg caacggtgtt tccttcatcg taatcgatga cggtaaattg tccgtcttcc
    11701 cctacaacaa catcggcagt ggcagtgtcg ccgaaaagat tgtgcatatt gccgagtatt
    11761 tcctgatatg ctcccaccat aaaaaagcct aaaagcggcg gctcttcttc gggataatca
    11821 ggcataggca tcgtaccggc gatgccgtct ccgtcgatgt agtggtcaat cgtaccgtct
    11881 gaatcgcagg taatgtccaa caacacggcg cggcgcgcaa tcggttcatt caaaccggta
    11941 atgggacaaa cagggaaaag ttgatctatg ccccaagcat cgggcaaaga ttggaagagt
    12001 gagaaattga catacagctt atcggcaaaa cgttcttgca attcgtcaat aatggttcgg
    12061 tgagaccggt gtttttcatt aaacaattcg ccgacttcat gacagatatt taaatacagt
    12121 tgctccgccc acgcacgttg cgccaaactc aacagcccga cattatactg attatgcaca
    12181 tcggcaagat caaactgccc ttcgtgtatc cagctgcgta aggaacgttt ttcccgcgag
    12241 gcggaaatat ccgtccaagt ttcccacata ctgtgcaaca cacgcggtgc ttcgggcgat
    12301 ggcgcatcca gccggcgcgg tttgtaacgt tcaacgccta taacattagc aaccaaaacg
    12361 gcgtgatgtg cggtaatgcc gcgcccgctc tcggtaatga ttgtcggatg cggcagcccg
    12421 tgttcgagac aagcctggct gatgccccat acgactgtgg aggcatattc gttgaggctg
    12481 taattaacgg aacaatccga ttgtgtgcgg tttccttcgt aatccacgcc aagcccgccg
    12541 cctacatcaa aacagcggat atttaccccc agtttgtgca actcaacata aaaccgagcc
    12601 gattcgtgta cacctgtggc aacatcacgg atgttcccaa gctgcgagcc taaatggaaa
    12661 tgcaaaagct gcaggcagtc cagcctgttt ttttgtttca aaatatcgac cagttgcaaa
    12721 acttgggaag ccgacaagcc gaattttgat ttttccccac ccgaagactg ccactttccc
    12781 gaaccttggg aagccagtct ggcgcgcaca cccaaacggg gcttgatgcc gagtttttcc
    12841 gcctcttcca ataccatttg tatttcggac agcttctcaa tcaccaaata aacctgatgc
    12901 cccagttttt cgcccatcaa ggcgaaacgg atatattcac ggtctttata gccgttgcag
    12961 acgattaatg tttgccgggt gccggcgtgt gccaaaaccg ccatcagttc ggctttagaa
    13021 ccagcttcca aaccatgcgg ttgtccgctt gacataagcg attcgatgac gcggcggtgt
    13081 tggttgacct tgatagggta aaccaaacaa taaccgccct tatagccgca ctcttcccgt
    13141 gccgtctgaa aggcgcggtt aatgtcgcgg aggcggtgtt cgaggatttg cggaaaacaa
    13201 aacaaaacag gcaggcgcgc ctgatgtttt tgttgcacgg cttcagtcag tttttgcagt
    13261 gaaacagttt gattgtgttg cgagggattg gggcggacga tgatttcgcc ggaatcgtca
    13321 acatcataat aacctatgcc ccaatgatta atgttgcaca cttcacggat ggtaaggata
    13381 ggcataataa acctgctccg tctgtcgtgt tgaaaaggaa tgattataac aaatcagcgt
    13441 gaaatgtcat ttttttaata agaaaagcct gcctcatacc tgatgaggaa caggcaaaat
    13501 gccgtctgaa cgcttcagac ggcattttgg ttcatctttc catcaaagga gtcagcgatc
    13561 gagctgctct ttgatgattt tcaggtcggg gtaagacaac acgatgtcgt attcgcggcc
    13621 gtctttatag gcttccactt ccagcacagg cttaccccaa tggtcgtcgg catcgacatc
    13681 gtgaacctga taaccgcgct gttccaacat tttcacagct tttacgcggt tctgttcaaa
    13741 attgggatca ctgtaaatct gacgctcggc agagtcgctg gcaaatgcgg ttgcggcact
    13801 cagggaaaca acggcagcca ataacagttt tttcattttc agtcctttct ttatcggttg
    13861 attgaataag atgtgttttt caatgccgcc attaaaacac agcaaaatta ggtttgaatt
    13921 agagggaagt caagaagcgt aaatgccgtc tgaaaaaaac agagccgtca aacggctccg
    13981 ttttccttat gcttctttgg tttcagccgc cttttggcgc aggcgcaggc tcaattcacg
    14041 caactgcttg tcgtccacgc tgttgggcgc gttggtcagc aggcattggg cacgttgtgt
    14101 tttcgggaag gcaatcacgt cgcggataga ttcggcgccg gtcatcaggg taaccaaacg
    14161 gtcgaggccg aatgcaaggc cgccgtgagg aggcgcaccg aatttcaggt tgtccaagag
    14221 gaagccgaat ttctcttgtt gctcttcagg gctgattttc agcgcggcaa acactttctc
    14281 ttgtacgtct gcacggtgga tacggataga gccgccgccg atttcccagc cgttcaatac
    14341 catgtcgtag gcacgtgcca aacaatttgc cgggtcggaa accatcaggt cttcatgacc
    14401 ttcttttggc gcagtaaacg gatggtgtac ggcaacgtag cggtcggctt cttcgtcgta
    14461 ttcaaacatt gggaagtcga caacccacaa aggtttccat tcgtctgtga aatagccgtt
    14521 ttccgcgccg tgttccaagc cgactttgat acgcaatgcg ccgatggctt cgttcacgac
    14581 tttggctttg tccgcaccga agaagatgat gtcgccgttt tccgcgccgg tacgcgcaat
    14641 gatttctttc agggcattct cagacaggaa ttttacgatt ggagattgca ggccgctgtc
    14701 ttcgccgttt gagaggttgc ccacatcgtt tactttgatg tatgccagac ctttcgcgcc
    14761 gtagatgccg acaaatttag tgtattcgtc gatttctttg cggctgaatt ttgcgccgtt
    14821 cggtacgcgc agggcgacta cacggccgcc tttcatatct gccgcgccac ggaagacttt
    14881 gaattcttcc gttttcatca ggtcggtcaa ctcggtaaat ttcaagttga tacgcatatc
    14941 cggtttgtca gagccgtagt agaacatggc ttcggcgtaa ggcatacgcg ggaagtcgcc
    15001 taaatctacg tttaaagcat ctttgaagac ttgtttcgcc ataccttcgg tgatgtccat
    15061 aatttcatcc tcgttcaaga acgaggtttc caagtcgatt tgagtgaatt caggctgacg
    15121 gtcggcacgc aagtcttcgt cacggaaaca tttggtaatt tgatagtaac ggtcgaaacc
    15181 ggccaccatc aacagttgct taaataattg cggtgattgc ggcagcgcga aaaactcgcc
    15241 cggatgaacg cggctcggca cgaggtagtc gcgcgcgcct tccggagtgg agcgggtcag
    15301 catcggcgtt tcgatgtcga tgaagccttg cgcgtccaga taacggcgaa cgcccatagc
    15361 gacttgataa cgcaggcgca ggttgcgttg catcaccgga cggcgcaagt cgataacgcg
    15421 gttggtcagg cgaacgtttt cgctgatgtt ttcatcgtcg atttggaacg gcggcgtggc
    15481 ggcggcgttc aagacttcga tttctttggc aaggatttcg attttgccgg aaatcatttt
    15541 gtcgttggtc gtaccttcgg gacggttgcg tacgcggccg gtaatgctca aaacgtattc
    15601 gttgcgagcg gaatcggcag cggcaaacgc ttcgggcgtg tcggggtcaa tcacgacttg
    15661 gacgatgcct tcgcggtcgc gcaggtcgat aaaaatcaca ccgccgtggt cgcgccgacg
    15721 gtgtacccag cctttgacgg taacggtttg gtctaagtat tgctcactga tcagaccgca
    15781 atagttggta cgcataaaat caccttttat tgatttaaac tgaaaacaga aaatgccgtc
    15841 tgaacggcgg ctttattgtt gttcgggcaa atccgccttt tcagacggca taggtcctgc
    15901 caatgttttg acgggcaggt cgtcagggat gaccataccc agcgaaatca catatttcaa
    15961 cgcttcgtcc acgctcatat cgagttcgcg cacatcgctt ttctttacca taatatagta
    16021 accgccggtc ggattcggcg tggtcggaac atacacggaa agataatcgc cgtccttcgg
    16081 caatgcggcc ttaaccgcat tcgacacctg accggacacg aatgcgattg tccaaatacc
    16141 cgattgggga aacggcacga gtactggtgt tttaaacgaa cggctgctgt cggacagcaa
    16201 cgattcggat acttttttca cactcgaata gatggacttc acaaccggaa tccgccccaa
    16261 caagctgtcc cacgcggcaa gaatctgccg gcccaatacg tttgcggcaa acaatccggt
    16321 tacaaacaat acggcaatgg caacgataac gcccagcccc gggatattaa accccaaaac
    16381 atattgcggc cgccattgct tcggcagcag gttgacgagc tgatcggacg cggaaacgat
    16441 ataggaaacc acccaaaccg ttaccgcaat cggcagccag accaaaatgc ccgtaatcag
    16501 atattttttt aacgccttgg cagctttacc gccttcggcc gcaggttccg tcatcttgct
    16561 tgattccgac aaagtccgta caaaccgcac attatacgcg tttgcccgga ttcaaacgaa
    16621 ttttttatcc cgccccgcca aaccgccggc ggcttcagac ggcacggcaa cttgatatac
    16681 cgtctgaaca cgcggttcag atgccgtcca agtcgttgaa catcaacccg ataccgatac
    16741 cgttctgctt gtggttgtag tcgatcagac tctcgccgta accgtggaat ccgcgtacca
    16801 cgcctttgag tttgccctta atcggaaacg tgtaggcggc ttcaatcgcg ccgtagcccg
    16861 ttttggggtt gtagcgcaat acggaataca cattctgcct gtcgttcagg cggtactgca
    16921 gcttcacgtc gccatacccc atatagtcgg caatatcggg attgtcgttt ttatcgccgc
    16981 tctgatcgaa cgcacgcacc cacacgcgcg gaatcaccgt caatttgccc cattccatgc
    17041 ctgccatggc ataaatcctg ttccacgaac gcgattcggg acggctctgt ccgttggact
    17101 ggtggacaaa acctgcaccg agcatacgca gcctgccgcc gaacggcaaa tctgccttca
    17161 caggctgggt caggaaaatt tcaggtttgt aatccgtatt gcggaacggc gcggatttcc
    17221 tgccttggtt gtaaatctgc caatcggatc tttgggtgta gccgaaccac agatccgcgc
    17281 gggttttaaa caaatcttcg gcaattttgc ttttgaacga aacctgcaat ttggtttccg
    17341 cacgtttctg ctgtccgaat ttttcctgta cagtcgtacc gcgcgtcggc gaacccgggg
    17401 catagttggg cgaattgttg taccagagcg gcataaggta catcggattg tgttcgcgta
    17461 cgcccaacag cccgcgcaaa tcgtttttgt ccaagtcgta catcaggctc aaaggcgtat
    17521 agatgtcggc ggtttcgccc gcactgtcgg caggaagcgc atccccgcct ttttcaacaa
    17581 caatgaccgc ctcgccctta tccaggctgc tgcggacggt ttccgtcaga ttgagtacgg
    17641 ctttcgactc ctgcccttcc taccctgccg aagacggaag ctgtgccgca aaaatcctgt
    17701 cgtaacacgc caaacgcgta acattgtccg tcaaagcggc gcattgcagc gcggtctctc
    17761 caaaagcgga tgccatcggc aacagtcctg tcaaaagaat atagcgcata ttccgtgtat
    17821 tcatctccgc ccccattgtc ggcatattgg ttttcaaacg gcattttata gcggattcgg
    17881 ataaaaaccg caccctttcc gccatttcgg gattttgccc ccgcaataca gaaaaccccg
    17941 aaaccgtcgg gcttcagggt tttccgctta tcgcgtatca accgccttgg cggttttgca
    18001 aaaatcaagc cagtgctttt acttttgcag acagacggct tttgtggcgt gccgctttgt
    18061 ttttgtggaa cacgcccttg tcggcgatgc ggtcgatgac tttgacggac tcttggtaaa
    18121 ccgcttgtgc ggcagcttta tcgcctgctt cgactgcttt caatactttt ttcactgcag
    18181 tgcggaatgc ggtacgcagg ctagcgttgt gggcgcgttg tttgacggac tggcgggcac
    18241 gtttgcgtgc ttgtgcgctg tttgccatat tggaatctcc tgaaagaaaa ataaattctg
    18301 taaacgcgca attttaaagg gggtttacat tatatgcaag ccttttctct ctatccgccc
    18361 gtcggacacc ctgacagcgc aaaaaaacgg caaaatcgtg cggacgtttt ctccctcttt
    18421 tgaaaacaag gtaatttttt aggaaaatca ggtcaatatc gctttgagtt tgcacaaatt
    18481 tactttacaa tgcccaaacc ttgcgccggg gtgtttatgg ggcgcggggt tttctttttt
    18541 tccaacctct ttcatcagga atctgcccgt atgaaaaaat ccgtattagc cgttttggcc
    18601 gcgctgtctc tggcagcctg cggcggcagc gaaaaaaacg ccgtgcaacc tcaagcagac
    18661 gctgcgtctg ctgccaatgc cgaagccgct gccaccgata ctctgaacat ctacaactgg
    18721 tcgaactatg ttgatgagag tacggtcgaa gactttaaaa aagccaacaa tctgaagctg
    18781 acttacgact tgtacgaaaa caatgaaacg ctggaagcca aaatgctgac cggcaaatcc
    18841 ggttacgatt tggttgtgcc gggcatcgcc ttcctgccgc gccaaatcga ggcgggcgcg
    18901 tatcaaaaag tcaacaaaga cctgattccc aactataaaa acatcgatcc cgaactcttg
    18961 aaaatgctgg aaaccgccga cccgggcaac cagtatgccg tcccctattt ctccggcgtg
    19021 aacacgattg cgattacggc gaaaggcaaa gagcttttag gcggcaagct gcccgaaaac
    19081 ggctgggatt tgctgttcaa acccgaatac acccacaagc tgaaatcctg cggcatcgcc
    19141 ctgtgggaca ccccgagcga aatgttcccg attttgctga actacttggg caaagacccc
    19201 aaaggctcga atcctgaaga tttgaaggcg gcggcggaag tgttgaagtc catccgtccg
    19261 gatgtcaaac gtttcagccc gtccatcatc gacgagctgg cgcgcggcga catctgcctg
    19321 gcggcaggta acggcggcga cttgaacttg gcgaaagcgc gttctgaaga agtgaaaaac
    19381 aatgtcggca tcgaagtgtt gacgccgaaa ggtatgggat tctggattga gtcttggctg
    19441 attcccgccg atgcgaaaaa cgtcgccaat gcccacaaat acatcaacta cacgctcgac
    19501 cccgaaatcg cggcgaaaaa cggtatcgcc gtaaccttcg cgccggccag caaacctgcg
    19561 cgcgaaaaaa tgcctgccga gctggtgaac acccgttcca tcttcccgaa cgagcaggat
    19621 atgaaagacg gtttcgtgat gccgcaaatg agtacggatg cgaaaaaact gtcggtcagc
    19681 ctgtggcaga aaatcaaagt cggcaccaac tgatttgaag cattaaaaat gccgtccgaa
    19741 catcgttcgg acggcatttt atattggatt gaaatagaaa tatttatata gtggattaac
    19801 aaaaatcagg acaaggcgac
//
LOCUS       DAWEWP010000024        19589 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWEWP010000024 DAWEWP010000000
VERSION     DAWEWP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19589
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.024"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(368..922)
                     /locus_tag="WGN53_000617"
     CDS             complement(368..922)
                     /locus_tag="WGN53_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139634.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            complement(1007..1507)
                     /locus_tag="WGN53_000618"
     CDS             complement(1007..1507)
                     /locus_tag="WGN53_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139635.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            1911..3107
                     /locus_tag="WGN53_000619"
     CDS             1911..3107
                     /locus_tag="WGN53_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ0139636.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            complement(3210..4454)
                     /gene="clpX"
                     /locus_tag="WGN53_000620"
     CDS             complement(3210..4454)
                     /gene="clpX"
                     /locus_tag="WGN53_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ0139637.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKGDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFEP"
     gene            4645..5016
                     /gene="rbfA"
                     /locus_tag="WGN53_000621"
     CDS             4645..5016
                     /gene="rbfA"
                     /locus_tag="WGN53_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ0139638.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            5049..5981
                     /gene="truB"
                     /locus_tag="WGN53_000622"
     CDS             5049..5981
                     /gene="truB"
                     /locus_tag="WGN53_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ0139639.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYAIDI
                     AEFDAPKAVISVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAVKNTGCV"
     gene            6272..8458
                     /locus_tag="WGN53_000623"
     CDS             6272..8458
                     /locus_tag="WGN53_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0139640.1"
                     /translation="MKADIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPAI
                     IGLLLGNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDSSDIVLDFPFKDCVLNGGQSSEEGEEIYFKHSNSQPASQPASQPASQPASQLYTKL
                     TRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRHTADGKQAVGEIKRHSDGTPAENLII
                     KGNNLIALHSLATQFKGKVKLIYIDPPYYFVKKKQQDSFGYNTNFKLSTWLTFMKNRL
                     SIAKELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFIGNLPTIMNLKGNNDEYAFA
                     GTHEYTLVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGFYKKGAPMRATGTEDKREDR
                     PEMFYPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQKLKEKYENLGYNFILPIAD
                     KQWGRWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRPELDDLPTKKPKTIFYKPEY
                     SSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNESDIVLDYHLGSGTTAAVAHKM
                     NRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQGGGEFVYAELAPFNETAKQ
                     QILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQEPEFQSLALDEQKQMVLEML
                     DLNQMYVSLSEMDDEQFAGCLNDDDKALSREFYQSAKNQAEKKDGE"
     gene            8448..11222
                     /locus_tag="WGN53_000624"
     CDS             8448..11222
                     /locus_tag="WGN53_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0139641.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQDELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQIHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRPDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSKYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEK"
     gene            complement(11371..12543)
                     /locus_tag="WGN53_000625"
     CDS             complement(11371..12543)
                     /locus_tag="WGN53_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ0139642.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            12832..13278
                     /gene="iscR"
                     /locus_tag="WGN53_000626"
     CDS             12832..13278
                     /gene="iscR"
                     /locus_tag="WGN53_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ0139643.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            13307..14521
                     /locus_tag="WGN53_000627"
     CDS             13307..14521
                     /locus_tag="WGN53_000627"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ0139644.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            14782..15168
                     /gene="iscU"
                     /locus_tag="WGN53_000628"
     CDS             14782..15168
                     /gene="iscU"
                     /locus_tag="WGN53_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ0139645.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            15255..15575
                     /gene="iscA"
                     /locus_tag="WGN53_000629"
     CDS             15255..15575
                     /gene="iscA"
                     /locus_tag="WGN53_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ0139646.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(15535..15756)
                     /locus_tag="WGN53_000630"
     CDS             complement(15535..15756)
                     /locus_tag="WGN53_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ0139647.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            15838..16338
                     /gene="hscB"
                     /locus_tag="WGN53_000631"
     CDS             15838..16338
                     /gene="hscB"
                     /locus_tag="WGN53_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ0139648.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            16436..19186
                     /gene="gyrA"
                     /locus_tag="WGN53_000632"
     CDS             16436..19186
                     /gene="gyrA"
                     /locus_tag="WGN53_000632"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ0139649.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            19343..19589
                     /locus_tag="WGN53_000633"
                     /pseudo
     CDS             19343..19589
                     /locus_tag="WGN53_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5483 a         4952 c         4594 g         4560 t
ORIGIN      
        1 ccttttccgc tttggcaacg attgaaaatt tccgtaaatt caaatatcta gattccttcc
       61 tgcacgggaa tgacacggaa gggtttcaga tgcagggtgg gcattcctgc ccacccaatc
      121 ccgcccttgc aacggtgggc aagaatgctc gccctatggt ctgacggttc gatatgatgc
      181 cgtctgaaaa cccaacggcg gcatgacaat gccgccctgc caacgcacgt aaatcagaat
      241 tgccatcccg acatcaaacg cttggaaaca aaatgccgtc taaaaatcaa acggcaacat
      301 aacaatgccc ctaacaaatg caaaaatgcc gtctgaaagc ccttcagacg gcattggcac
      361 gccggattta ccgccttctg ccgaaaccgc gcatggcggg gcggcggtaa ttggcgggcg
      421 ggcgcgttgt cgggcggtaa cgctgcgcct gcgccgcctg ttgttttgca cggaggctgc
      481 gcgtgttcaa atccctgctg gtgcgcgcat tgggatgtgc ggactgatgg taggctgcac
      541 gcgcgcgccg ggcgacggga ctgtcttggt tgcctgcccg tgtgaatttg tttgccagcg
      601 cgttgccgat aaacgcgcct gccgccgcgc cgactaggct ttgcagcagc cagcttcctg
      661 tcgattggtc gtaaatatac tgctgcccgt ctttaccggt aacgggttgc ccgttgttgc
      721 cgtttgcctg tgcttcggca ggaatggtgt ctttgactgc ttcgggagtc agttggtaaa
      781 ccgtatcgtc tgcctgctgt gcgagctgct gttgcagggc ttcaatctgt ttctgctgct
      841 gttcgagccg cgcctgcgtg tcgtcttggc aggcggcgag tgcgaatgtt gcgataagcg
      901 cggaggcgat gatttttttc atgtgtgtcc tgtttgggtg gaaaatcggt tttattgtat
      961 cgccgtcggg aattttggca agcattctgc cgacaaatcg tgatgtttac aggggcaggg
     1021 tgtgcagttt gcggacaaat gcgaggctgt tggcgactgg gttgcctttg ttttcgactt
     1081 cgtgttcggt ttctacggtc agcaggcggc ggtttttgtg tttgttcagg ctcaattcgg
     1141 cttgtgcggg tgtgaaaaac aggcggtgtt tttcggcaaa gcggcgggcg cggctgttgg
     1201 cggttttcaa aatctgaagc agcgatacgt ccagatggaa gcgtcgcacg cccaatacga
     1261 gaatccgtgc ggcaaaaaaa tcggcggcat cggtaaactc ggcggggctg ctgcggctga
     1321 agtcgtgccg ttcggcggct atcagggcgg caacagtgcg gatttgcgga atcatcgatt
     1381 cgctgataag tgtgtcgtcc cgccctgcat cgagaagcat gggggaaaaa tcctgtgcat
     1441 catcgacaat aatgctacaa gtgtgcaggg tttcgttttg tgcggcggtt tggggcattg
     1501 cattcatggt cattttcctg attctgtcgt gtgttgccga atcgggcgac ctgtgtgaag
     1561 gtaacaaaaa agccgccccg ttttcgagcg gcctgttttg cgtatgggat ggatttcaag
     1621 caagcgcaaa aaagtaccgc acgtctgtgt ggtaccaata gcaataagcg gttgtaaatt
     1681 ttttgccttg catgatgaaa tgccgtctga agataaaaat attggggaga ttctaaatca
     1741 aaacgctgcc gcgcctcaag cattttatcg aaattttttt gatttttcat ctatccgatt
     1801 gaaaatattt cggtttattt ttaccgctgc ccgatattgt cggcaatttc cctttatctg
     1861 ctttgaaaaa cggtgcataa tcccgagcaa aaccgcaatc aggagcaatt atgcaaaact
     1921 atctgacccc caatttcgcc tttgccccga tgattcccga acgcgcttca ggcagccgcg
     1981 tttgggatac gaaagggcgt gaatatattg atttttcagg cggtatcgcc gtcaatgcgc
     2041 tgggacactg ccaccctgcc cttgtcgatg ctttaaacgc gcagatgcac aagctgtggc
     2101 acatttccaa tatctatacg acgcgtccgg cgcaggaatt ggcgcaaaaa ttggttgcaa
     2161 acagttttgc cgacaaggtt tttttctgca actcgggcgc ggaagcgaat gaggcggcgt
     2221 tgaagctggc gaggaaatac gcccgcgacc gtttcggcgg aggaaaaagc gaaatcgtcg
     2281 cctgtatcaa cagttttcac ggacgcacgc tgtttaccgt gtccgtcggc ggtcagccga
     2341 aatacagcaa ggattatgca cccctgccgc aaggcattac gcacgttccg ttcaacgata
     2401 ttgccgcgct ggaagctgcc gtcggcgaac agacctgcgc ggtcatcatc gagccgatac
     2461 agggcgaaag cggcatcctg cccgccactg cggaatattt gcaaaccgcg cgccgtctgt
     2521 gcgaccggca caatgcgttg ttgattttgg acgaagttca aaccgggatg gggcatacgg
     2581 gcaggctgtt tgcctatgaa cattacggca ttgttcccga tattttgagt tcggcaaaag
     2641 ccttgggctg cggctttccg atcggcgcga tgctggcgac agaaaagatt gccgccgcct
     2701 tccaaccggg cacgcacggc tcgactttcg gcggcaaccc gatggcgtgt gcggtcggca
     2761 gccgcgcatt cgacatcatc aatacgcccg aaactttaaa ccatgtccgt gaacaggggc
     2821 agaaacttca gacggcattg ctggatttgg gcaggaaaac gggcttgttc tcacaagttc
     2881 gcgggatggg gctgctactc ggctgcgtgt tggacgaagc ctatcgcgga cgcgcatccg
     2941 aaatcaccgc cgccgccttg aaacacggcg tgatgatttt ggttgcgggt gcggacgtat
     3001 tgcgtttcgc gccttcgcta ctgttgaacg atgaggatat ggcggaaggt ttgcgacgtt
     3061 tggaacacgc gctgacggaa tttgccgcga catcagacaa tccgtaaaac tcaaatgccg
     3121 tctgaaggcg ggaaggcttc agacggcatc aaaaacaaaa aaccgcttcg gaaacgtggt
     3181 tcgacgttcc gaagcggttt tgtttgccat cagggctcga acaccaattc cggttccctg
     3241 ccctcttcga tgactgcctt accgaccacc acttttttca agcctttcaa atcgggcagg
     3301 cggtacatcg tatccaacag gcagcgttcg acgatggaac gcaggccgcg cgcgccggtt
     3361 ttgcgctcca ttgcctgacg cgcgatggaa cgcaatgcgc cttcttcaaa ctccagttcg
     3421 acattttcca taccgaacaa ggcttgatac tgcttgacca aagcattttt cggctcggtc
     3481 aaaatattaa tcagcgcgtc ctcatccagt tcttctaacg ttgcaatcac aggcaaacgt
     3541 ccgattaatt ccggaatcag accgaatttg attaaatctt ccggttcgac gatgccgaac
     3601 agcttggtaa tgtcggcatt ttcgtccttg ctgtgaacgg acgcaccgaa accgataccg
     3661 cctttctcgg tgcgctggcg gatgactttt tccaagcctg caaacgcacc gccgcagata
     3721 aacaggatgt tggtggtatc gacgttgata aattcctgat tcggatgctt gcggccgcct
     3781 tggggcggaa cacttgccac cgtaccttca atcagtttca gcaaggcttg ttgcacacct
     3841 tcgccggaca cgtcgcgcgt gatggacgga ttgtcgcctt tgcgtgaaat tttatcgatt
     3901 tcgtcgatat agacgatgcc gcgctgggct ttttcaacat cgaaatcaca tttgcccaac
     3961 agtttggtga tgatttgctc gacatcttcg ccgacataac ctgcttcagt cagcgtggtt
     4021 gcatccgcca tcacgaacgg tacgtccagt ttacgcgcca aagattgcgc cagcagggtt
     4081 ttacccgatc cggtcgggcc gataagcagg atgttggatt tcgacaattc gacattagct
     4141 ccggctttcg ggtggcgcag gcgtttgtaa tggttgtaca ccgacaccgc caaggctttc
     4201 ttggcttgtt cctgaccgat aacgtggtcg ttgaggttgg cgacgatttc ggcgggcgtg
     4261 ggcagcttgc cggattcttc cggctcccct ccggcacttt ccgaaggtgt gccgtcattt
     4321 ccgtcttcat gcaatatttc aatacagttt gagacgcatt cgtcacagat aaaggcgttt
     4381 tcgccctcaa ttaaatgttt gacgtgtgat ttggattttc cgcaaaagga acaggtacgg
     4441 ttttcgttgg acatggcttt ctttacaatg tatgcgttac agaaaacggc acgtgccgtt
     4501 cgggttgcca agtataataa ctatatccgt tcttatcaat gtattacctt aaaatcccgc
     4561 cgattaggct ataatacgcc ctttcgcaac cgccccggcg gcaaaaatgc cgtctgaaac
     4621 caaatctgaa atctgaggat attcatgaga aaaccccaac gcggctacgc ccgccaagac
     4681 cgtgtcaaag aacaaatcat gcgcgagctt gccgaactcg tccgtaccgg actgaaagac
     4741 ccgcgcgccg gcttcattac cgtcaacgaa gtcgaagtta cccgcgatta cagccacgcc
     4801 accgtgttct acaccatttt aaaccaagac gcgcgcgaaa ttacggaaga agtgctggaa
     4861 cacgcgcgcg gacacctccg cagcgaattg gccaaacgca tcaagctgtt caaaacgccc
     4921 gaactgcatt tcaaatacga cgaatctttg gaacgcggtt tgaacctgtc cgcccttatc
     4981 gaccaagtag cggcggaaaa accggttgaa gactgacgga tatgcccatg ccgtccgaac
     5041 atcgaaccat gaatacaggc aaaccccaaa aacgtgccgt caacggtgtt ttgctcttgg
     5101 acaaacccga aggcctttcc agcaacaccg cgctgcaaaa agcgcggcgt ttgtttcatg
     5161 ccgaaaaagc cggacatacc ggcgtgctcg accctttggc aaccggactt ttgcccgtct
     5221 gcttcggtga agcgaccaag ttcgcccaat acctgctgga tgccgacaaa gcctacaccg
     5281 ccacgctgaa actcggcgaa gccagcagca cgggtgatgc cgaaggcgaa atcattgcca
     5341 ccgcccgcgc cgatatttcc ttagccgaat ttcagacggc ctgccaagca ctgacaggca
     5401 acatccgcca agtgccgcca atgttttccg cgctcaagca cgaaggcaaa ccgctgtacg
     5461 aatacgcccg caaaggcatc gtcatcgaac gcaagccgcg cgatattacc gtttacgcca
     5521 tcgatattgc cgaatttgac gcacccaaag ccgtgataag cgtgcgttgc agcaaaggca
     5581 cctatatccg caccctcagc gaagacatcg ccaaacacat cggcacgttc gcccacctga
     5641 ccgccctgcg ccgcaccgaa accgccggct ttaccatcgc ccaaagccac acgctcgaag
     5701 ccttggcaaa tttgaacgaa acagaacgcg acagcttgct gctgccctgc gacgtattgg
     5761 ttgcccattt tccccaaacc gttttaaacg attatgccgt ccatatgctc caatgcgggc
     5821 aacggccgcg tttcgaggaa gacctgcctt ccgatacgcc ggtacgcgtt tacacggaaa
     5881 acggccgctt tgtcggactg gcggaatatc aaaaagaaat atgccgtctg aaagccttgc
     5941 gcctgatgaa cacggcggtt aaaaatacgg gctgtgtttg aataatgtgt tgatatagtg
     6001 gattaaattt aaaccagtac agcgttgcct cgccttgccg tactatttgt actgtccgcg
     6061 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ttccgcaaaa tcacgacaca
     6121 ctcggacacc caccccgctt attgcaactt tgcgaacgcc cccggaaaca gcaaagataa
     6181 caaatgattg attttattag tatttatttg caaaaccatt tgccgttaca caagaatggc
     6241 acattaaaat gactgatgag gatttataac gatgaaggca gacattcaaa ccgaattaac
     6301 ccaagccctg ctatcacacg aaaaagtatg ggcgaacgaa gaaaaaacca ttttagccaa
     6361 aaacattctg ttggatttgg tggaaaaaac cgacccggcc attatcggtt tgttattggg
     6421 gaatgatgag ttaaaacgcc atttttttgt ggaagtgaat ggtgttttgg tgtttaaatt
     6481 gcaggatttt cgttttttct tggacaaaca cagcgtcaat aattcctaca caaaatacgc
     6541 caaccgcatt ggtttgacgg acggcaaccg ctttttgaaa gacagttcgg atattgtgtt
     6601 ggattttccg tttaaagatt gcgtattgaa tggcgggcaa agcagcgaag aaggcgaaga
     6661 gatttatttt aaacacagta atagccagcc agccagccag ccagccagcc agccagccag
     6721 ccagccagcc agccaattat acactaaatt aacccgaaaa agacaagaaa tcttttttaa
     6781 tcaaacgctt gcttttgatg aaattgaccg gctttttgac gcaaaagcat tctcaaaatt
     6841 ctctcgccac accgcagacg gcaaacaagc ggttggcgaa atcaaacgac attcagacgg
     6901 cacaccagcc gaaaatctca ttatcaaagg caataatctg attgccctgc attcgcttgc
     6961 cacgcagttt aaaggtaaag tgaagctgat ttatattgat ccgccttatt attttgttaa
     7021 aaagaaacaa caagatagtt ttggttataa cactaacttt aaattatcta cttggcttac
     7081 atttatgaaa aatcgtttat caattgccaa agaattatta actgatgatg ggattattgt
     7141 tatttccatt gatgatgacg gaaatgctta tttaaagatt ttgttagatg aaatttttgg
     7201 atttgaaaat tttatcggaa atttacccac tattatgaat ttaaaaggta ataatgatga
     7261 atatgctttt gcaggcactc acgaatacac actagtcttt gctaaaaata aagataaatc
     7321 tactttttat gaatttccaa tagatgaaga taatttttta gagaaatggg aagaagatga
     7381 aattgggttt tataaaaaag gagctccaat gcgagcaaca ggaaccgaag ataaaagaga
     7441 agatagacct gaaatgttct atcctttttt ggtaaaaaat aacacagtta gtacaattac
     7501 agatgaagaa ttttctcaaa tctataataa agatttagaa gtattcaatg atgattttat
     7561 tcaaaaacta aaagagaaat acgaaaatct tggatacaat tttattttac ctattgctga
     7621 taaacaatgg gggcgttggc gttgggggta ttcaataaag aataaggcaa ggctgcagac
     7681 ggatattatt gttagccaat ctaaaaatgg aatttccttg tataaaaaac aaagacctga
     7741 attagatgat ttacctacaa aaaaacctaa aactattttt tataaacctg aatacagtag
     7801 tggtaatgga actgaacaga tgaaaaatct ttttggtgaa aaggcattta aaaatcctaa
     7861 accagaagaa cttatccaag attttattac catcacaacc aacgaatccg acatcgtctt
     7921 agactaccat cttggtagtg gcacaaccgc cgccgttgcc cacaaaatga accgacaata
     7981 tatcggtatt gaacaaatgg attatattga aacgcttgcc gttgaacgct tgaaaaaagt
     8041 gattgatggc gagcaaggcg gtatttccaa agccgtgaat tggcaaggtg gtggcgagtt
     8101 tgtttatgcc gaacttgccc catttaacga aaccgcaaaa caacaaattt tggcttgcga
     8161 agattcagac ggcatcaaaa cgctgtttga aggtttatgc gaacgctatt tcttgaaata
     8221 caacgtcagc gtaaatgaat ttagtcaaat cattcaagag cctgaatttc aatccttggc
     8281 attagacgaa caaaaacaaa tggtgcttga aatgttggat ttaaatcaaa tgtatgtttc
     8341 attatccgaa atggatgatg agcaatttgc aggttgctta aatgatgacg ataaagcgtt
     8401 aagccgtgaa ttctatcaat cagcaaaaaa tcaagcggag aaaaaagatg gcgaataata
     8461 aaacgttatt tgattgggtg gaagaccgca aatcaacgct ggaagaaata gaacagacag
     8521 attttttcgc gctgcctgaa tttgtagaac gcaatttaaa atatccgttt tttgaatggc
     8581 aaaaatcggc tttagaaaac tttgtgattt ttgaccgcgc ttcaaaatta aaggatttcc
     8641 ctgacatcaa aaaccgccct acccatttgc tgttcaatat ggcaacaggt gctggcaaaa
     8701 cgatgatgat ggcggcgttg attttgtatt attttgaaaa aggttatcgg cattttttgt
     8761 ttttcgtcaa tcaaaataat atcgtggata aaacggaaaa taattttatt gacccggcgc
     8821 acgcaaaatt tttatttacc gagaagattt tgcaaggtga tacggtaatt cctattcgca
     8881 aagtggaaat gttcagccca cattcagacg gcattgaaat taaatttacc agcattcaaa
     8941 aactgtataa cgatatccac accgagcgag aaaaccaaac cacattggca gatttgcaca
     9001 aattgaacct tgtgatgctg ggcgatgaag cacaccattt aaacgcacaa accaaaggca
     9061 aaaaacaaga cgagttggat ttggaagtgg aattaaaagc caatgcaggc aatgccgaaa
     9121 ttgaacgcaa aggctgggag catatggttt tggaattgtt gctcaataaa aatggcaatc
     9181 ccggccaaaa tgtgctgttg gaatttaccg ccacgctgcc tgaaaatgct gacgtgcaac
     9241 aaaaatatgc ggacaaaatc atcacaaaat ttggcttaaa agagtttttg caaaaaggtt
     9301 ataccaaaga aatcaatttg gtatccagta cgctgaataa gaaagagcgg gtgttgcacg
     9361 ctttattatt tgcttggtat cgccatcaaa tcgcgttgaa atacggcatt gccaatttca
     9421 agcctgtgat gttgtttaga agtaagacga ttgatgaatc aaaagcggat tattcggcat
     9481 ttttaaattg ggcagaaaat gtacaagcgg atgattttga gtttttagat acgttttcag
     9541 gcagcctgaa taatagcgac aacgccaacg aacaaggcaa aacccgaacc gaacaagccc
     9601 taaaatttat gcaggaaaat ggctttgagt ttgcacattt ggcaaattgg gtgaaacaaa
     9661 attatcaaaa acataatgtg attattacca actccgaaac caacaaaaac aaaactgaaa
     9721 aaaccgacag cgaaaccgaa aaattgctaa acaatttgga agcggctgat aatccaatcc
     9781 gtgccatttt tacggtagat agattaaccg aaggttggga cgttctgaat ttgtttgata
     9841 ttgtgcgttt gtatgaaggg caaaatggcg gtggttcaaa taaaaaatca ggcaaaacgg
     9901 ctgccgctac cgtatcggaa aagcagttga ttggtcgtgg cgtgcgttat tttccatttg
     9961 cgtttgaagg taaacagccg aataaacgca aatttgacaa cgacatgcaa cacgaattgc
    10021 gtatcttgga agaattgttt tattacacgc acgatgagca atcccgctat atttcagaac
    10081 tgaaaaatga gttgcgaaaa gacggttatt tgcctgaaaa agacgatgat aaggtgttgg
    10141 caacatttaa actcaaatct gaatttgccg ataataagga ttttagagag ttgttaattt
    10201 gggcgaataa aaaaatcccc aacccaaacg ccaaaagcaa taatgcagac agcctgcaag
    10261 ccaatccgca aacgctttca ttccaaattc acggcaatca attgttgcag gaaacgcaat
    10321 ttacagccga tgaaaatgat gaaacagccc gacaaatcgg cacacaaaat aattttactc
    10381 aaaccataaa aatgagtgaa atggagcggc acattttcaa taaagcatta catatcaaag
    10441 ggaaaaatag tcaatcttta ttccattttg accgcctgca aagcaaactc aacattcaaa
    10501 atcgcaatga attgcaaaat aacttgttga aagactggca aattgaattt ttaggattag
    10561 ggcaagacaa acaggttcgc ccggatgata aacttgcagg ctgcctgaaa atcttggaaa
    10621 tggttgaaaa acatctgaat gaaagtgata tgccgtttat cggtacaaaa gaatttacac
    10681 ctaaaaaatt atgggaaatt ttcggcacgc caaaacaaaa atgggtcaaa aaagatgatg
    10741 taaaaacgac tattgccacg caaaatgatt ggtatgtgat ggataatttt gctggaacgg
    10801 gtttggaaga agcgttaatt caatttattt cagcgcgttt gggcgatttg aaatctaaat
    10861 atgacgttca tttaatccgt aatgaagaag tgtttaaatt gaataacttt gccgatggtg
    10921 aaggatttat gccggacttt attttattgc tgaaagataa acaaaaatct tcttccaatg
    10981 gtgtgaacga ctttttgcat taccaaattt tcattgagcc caaaggtgag catttagtgg
    11041 aaactgatgg atggaaagaa gaatttttag aagccatcac tgtggaatat gggatggata
    11101 agatcctgca aaaagataca ccgcattatc gtttgatcgg tttaccgttt tttactgatc
    11161 atcagaaaaa tggacaattt acggagtcat tccctttagg tgcggcatcg cttgaaaaat
    11221 agagtagtgc attgcaggca accccgtttg acaaaacttc ctttacaaaa gggcgttttg
    11281 tcagatattt aatcaacaca ttattaaaat acagccaaat tttaatgccg tccgaaccct
    11341 gtgttcagac ggcatcgtat ttttcagtat ctaaaccgtt tccctacccc aatctttacc
    11401 ccttaaaatc gaggcatcga catcttgaat atcgcggtgt cccgtaaacg ccatagatat
    11461 atccatttct ttatatagaa tttccagcgc acgggttacg ccttcttcgc catacgcgcc
    11521 caagccatac aggaacgccc gacctatcat tgtacctttc gcgcccaaag cccacgcctt
    11581 caaaatatcc tgaccgctgc ggatgccgct gtccatccaa acttcgatgt cgctgcccac
    11641 tgcgctgacg atgtcgggca aggctttgat ggcagacacg gtatcgtcga gctgtcggcc
    11701 gccgtggttg gaaacaatca atgcgtccgc gccgcttttc gctgcttttt ccgcgtcttc
    11761 gggttccata atgcctttga taatcagctt gccgccccac aaatctttaa tgcgcgccac
    11821 atcgtcccag ctcaggcgcg ggtcgaattg ttcggaagtc catgaagaca gcgaagacaa
    11881 atcgccgacg ttcttcgcgt gtccgacgat attgcggaac gtgcggcgtt ccgtgttcag
    11941 cattttcatg caccattccg gtttggtcgc cagattgatt aaattggcga tggtcggttt
    12001 cggcggcgcg gacaggccgt ttttgatgtc tttgtggcgt tgacccaaaa cctgcaaatc
    12061 ggcggtcaat accaatgccg aacatttggc atccttcgcg cgcttaatca ggttttccat
    12121 aaactcgcgg tcgcgcatca cataaagctg aaaccaaaac ggcgcgctgg tgttctcggc
    12181 aacgtcttca atcgagcaga tggacatggt ggacaacgta aacggaatac cgaacttctc
    12241 cgccgcccgc gccgccaaaa tttcgccgtc tgcgtgtgcc atgccggtaa aacccgtcgg
    12301 cgcaatcgcc accggcattt tcacatcctg cccgatcatt ttagtctcaa ggctgcgtcc
    12361 ctccatattg accaatactt tttgacggaa gcggatgtct ttgaaatccg aagtattttc
    12421 ccgataagtc gtttccgtcc acgaacccga atcgatgtaa tcgtaaaaca tacgcggcat
    12481 tttgcgcttg gcaacgcggc gcaagtcttc gatgcaggtc attttgctca aatcacgttt
    12541 catttgtcgc ccctgaatac ctgaataact ttatatgaaa tagataatgt atatcaatat
    12601 tgattataag gcaaatcatt tcaacattta ctgcacccgc cgccgctccc acttcaagcg
    12661 gcataaggtt tagaattcaa aaataatata ttaaaatcaa aatattaaaa atcaatcaat
    12721 ctgccgattt aaacagccaa tcgcgcaatc cgcccccata cttgactgaa acactcagat
    12781 attggacaat tccgcccact ataaaaaaag ccgacacggg caaccaccac catgagactg
    12841 accaccaaag ggcgtttcgc cgttaccgct atgctggatt tggcgatgaa cgcgcaaacc
    12901 ggcgccgtca aactcagtgc catcagcgaa cgccaaaaca tatccctctc ctatctcgag
    12961 caattgttcg gcaaactccg ccgcgccgga cttgttgaaa gcctgcgcgg gcccggcggc
    13021 ggctacatcc tcgccgcacc ggcggcacgc atcaacatcg cccaaatcat cgccgccgcc
    13081 gaagaccggc tggacgcgac ccaatgcggc agcaaagcca actgccacca cggcgcgccc
    13141 tgcctgacgc acgacctttg ggagaattta aacaaaacca tcaacgacta cctcggcagc
    13201 gttaccctgc aaagcatcat cgaacagaaa aacaacggcg acggcagccg cgtcgtccaa
    13261 tttacacaca tccattaaat aacacccgaa aaagaaaggg caaaccatga ccgtcaaaac
    13321 ccccgtttac ctcgactacg ccgccaccac ccccgttgac aaacgcgttg ccgaaaaaat
    13381 gattccctat ctgaccgaaa ccttcggcaa ccccgcctcc aacagccacg cattcggctg
    13441 ggaagcagaa gaggccgtcg aaaaagcccg cgccgacatc gctgccctga ttaatgccga
    13501 ccccaaagaa atcgtcttca ccagcggcgc gaccgagtcc gacaacctcg ccatcaaagg
    13561 cgcggcaaac ttctacaaaa ccaaaggcaa acacctcatc accgtcaaaa ccgaacacaa
    13621 agccgtttta gatacaatgc gcgaactcga acgccaaggt tttgaagtaa cctacctcgg
    13681 cgttcaagaa aacggtttga ttgatttgga agaactcaaa gccgccatcc gcgacgacac
    13741 catcctgatt tccgtaatgt gggcaaacaa cgaaatcggc gtggtgcaag acattcccgc
    13801 catcggcgaa atctgccgcg aacgtaaaat cgtcttccac gtcgatgccg cccaagcctg
    13861 cggcaaagtg cctgtcgatg tcgaagccgc caaaatcgac ctgctctcga tgtccgcgca
    13921 caaagtgtac ggccccaaag gcatcggcgc gctgtacgtc cgccgcaaac cgcgcgtccg
    13981 cctcgaagcc caaatgcacg gtggcggcca cgaacgcggc ttccgcagcg gcacattacc
    14041 gacccaccaa atcgtcggta tgggcgaagc cttccgcatc gccaaagaag aactcgaaca
    14101 agatatggcg cactaccgca aactgcgcga catcttcctc aaaggcatcg aaggcatcga
    14161 agaagtctat atcaacggcg acctcgaaca ccgcgccccg aacaacctga acgtcagctt
    14221 caacttcgtc gaaggcgaaa gcctgattat ggcagtgaaa gaactcgccg tatccagcgg
    14281 ctccgcctgc acctccgcct cgctcgaacc cagctacgtc ctgcgcgcat tgggcagaaa
    14341 tgacgaattg gcgcactcgt ccctgcgcat caccttcggt cgcatgacca ctgaagaaga
    14401 agtgcaattt gccgccgaac tgattaaatc caaaatcggc aaactgcgcg aactgtcgcc
    14461 gttgtgggaa atgttcaaag acgggattga tttgaactcg attgaatggg cggcgcatta
    14521 aagcgtacca acatgccatc cgaaccttca gacggcattc caaaaacaaa gcaatcaaga
    14581 gaaaaatatg aacgaacaag atttagattt ggacaacctc gacaacctgc ttgacgattt
    14641 cgacggcgtt accgtggaag gcggcgtcga ttcggaaaac gacgacggct gtgaaggcgg
    14701 agcgtgcaaa atctaactca ggccgtctga aaaccggcaa acaaaccact taaatcatca
    14761 aaacattaag gaaaccacat catggcatac agcgataaag taatcgacca ctacgaaaat
    14821 ccccgcaacg tcggtacttt cgacaaaaac gacgaatctg tcggcaccgg catggtcggc
    14881 gcaccggcct gcggcgacgt gatgcgcctg caaatcaaag tgaacgatga aggcatcatc
    14941 gaagatgcga aattcaaaac ttacggctgc ggttccgcca tcgcttcgtc cagcctgatt
    15001 accgagtggg tcaaaggcaa aagtctggat gacgcgctgg caatcaaaaa cagcgaaatc
    15061 gccgaagagt tggaattgcc gccggtaaaa atccactgct ccatcttggc tgaagatgcg
    15121 gtaaaagcgg ccgttgccga ctaccgcaaa cgtcaggaaa acagataaag cctttcagac
    15181 ggcatcgtcc cgcaatgccg tccgaaccac ccgccgcttc aggtccgtcc ggggcggtac
    15241 aacaaggaag aaatatgatt acccttaccg agaatgccgc aaaacacatc aatgactatc
    15301 tcgccaaacg cggcaaaggc ttgggcgtac gcttgggtgt gaaaaccagc ggctgctcgg
    15361 ggatggcgta caaccttgaa tttgtcgatg aagccgatgg cgacgacctg attttcgaag
    15421 gacacggcgc gcgcatttat atcgatccga aaagcctggt ttatctggac ggcacgcaag
    15481 tcgattacac caaagaaggt ttgcaggaag gtttcaaatt tgaaaacccc aatgtcaaag
    15541 actcctgcgg ctgcggcgaa agcttccacg tttaaggcat aaaaacggcg ggacagtatc
    15601 aaaaccgtcc cgccattttt tcgcttcctg cctgttgtag ctgcctttgc ctttcctttt
    15661 ccgttccacc ttgtgccgga acaaatcgga tttcactaag gcttttaaag cattgtcgcg
    15721 tattttgcct ttattgtgct gcactttgcc gcccatattc agtcctttcg tttaagaagc
    15781 tgcagattat aaggcaaaaa cagttttctg ccaaaatctt acatttatca ttctactatg
    15841 tcccaatatt tcaccctctt ccggattgaa cccgctttcg atatcgacac cgaaaacttg
    15901 gaacaaacct accgcgcctt ggccgcccgt ttccatcccg ataaattcgc ttcagcttcc
    15961 gcctttgagc aaaagcaggc agtgatgatg tcttccacca tcaacgatgc ctaccgcacc
    16021 ttgaaaaacc ccatcgaccg cgccgcctac ctgctgaaaa catcgggcat cgatgccgac
    16081 gcgccggagc ataccgcttt cgcccccgaa ttccttatgc agcaaatgga atggcgcgaa
    16141 acgctgatgg aggcacgggc aggcaacgac cttgaatcct tgaaaaatct cgacaacgaa
    16201 atccgcgacg aacaagaaaa actgttctgc ggtctgaaac agtcgtttgc ccgacaagat
    16261 tacgacacag ccgcacaaca agtccgccaa ggcaggtttc tcgacaaact ccgcaacgaa
    16321 atttcctcgg cattataatc cgcaccgtgt ttcagacggc gtaaccgccg caccgttccg
    16381 cgtcaaaata tgctaaaata agcaacaatt ttttgccata cgaaacattg aaaccatgac
    16441 cgacgcaacc atccgccacg accacaaatt cgccctcgaa accctgccgg taagccttga
    16501 agacgaaatg cgcaaaagct atctcgacta cgccatgagc gtcattgtcg ggcgcgcgct
    16561 gccggacgtt cgcgacggtc tcaagccggt acaccgccgc gtactgtacg cgatgcacga
    16621 attgaaaaac aactggaatg ccgcctacaa aaaatcggcg cgcattgtcg gcgacgtcat
    16681 cggtaaatac cacccccacg gcgataccgc cgtatacgac accatcgtcc gtatggcgca
    16741 aaatttcgct atgcgttatg tgctgataga cggacagggc aacttcggat cggtggacgg
    16801 gcttgccgcc gcagccatgc gctacaccga aatccgcatg gcgaaaattt cccacgaaat
    16861 gctggcagac attgaggaag aaaccgtcaa tttcggcccg aactacgacg gtagcgaaca
    16921 cgagccgctt gtactgccga cccgtttccc cacactgctc gtcaacggct cgtccggcat
    16981 cgccgtcggc atggcgacca atatcccgcc gcacaacctt tccgataccg tcaatgcctg
    17041 cctgcgcctg ctcgatgcac ccgacaccga aatcgacgaa ctgatcgaca ttatccaagc
    17101 ccccgacttc ccgaccgggg caaccatcta cggcttgagc ggcgtgcgcg aaggctataa
    17161 aacaggccgc ggccgcgtcg ttatgcgcgg taagacccat atcgaaccca taggcagaaa
    17221 cggcgaacgc gaagccatcg ttatcgacga aatcccctat caggtcaaca aagccaagct
    17281 ggtcgagaaa atcggcgatt tggttcggga aaaaacactg gaaggcattt ccgagctccg
    17341 cgacgaatcc gacaaatccg gtatgcgcgt cgttatcgag ctgaaacgca acgaaaatgc
    17401 cgaagtcgtc ttaaaccaac tctacaaact gactccgctg caagacagtt tcggcatcaa
    17461 tatggtggtt ttggtcgacg gacaaccgcg cctgttgaac ctgaaacaga ttctctccga
    17521 attcctgcgc caccgccgcg aagtcgttac ccgacgtacg cttttccggc tgaagaaggc
    17581 acgccatgaa gggcatattg ccgaaggcaa agcggtcgca ctgtccaata tcgatgaaat
    17641 catcaagctc atcaaagaat cgcccaacgc agccgaggcc aaagacaaac tgcttgcgca
    17701 cccttggcgc agcagcctcg ttgaagaaat gctgacgcgt tccggtctgg atttggaaat
    17761 gatgcgtccg gaaggattgg ctgcaaacat cggcttgaaa gagcaaggtt attacctgag
    17821 cgagattcag gcagatgcta ttttacgcat gagcctgcga aacctgaccg gcctcgatca
    17881 agaagaaatt gtcgaaagct acaaaaacct gatgggtaaa atcatcgact ttgtggatat
    17941 cctctccaaa cccgaacgca ttacccaaat catccgcgac gaactggaag aaatcaaaac
    18001 caactatggc gacgaacgcc gcagcgaaat caacccgttc ggtggcgaca ttgccgatga
    18061 agacctgatt ccgcaacgcg aaatggtcgt taccctgaca catggcggct atatcaaaac
    18121 ccagccgacc accgactatc aggcgcagcg tcgcggcggg cgcggcaaac aggcggctgc
    18181 caccaaagac gaagacttta tcgaaaccct gtttgttgcc aacacgcatg attatttgat
    18241 gtgctttacc aatttgggca agtgtcattg gattaaggtt tacaaactgc ccgaaggcgg
    18301 acgcaacagc cgcggccgtc cgattaacaa cgtcatccag ttggaagaag gcgaaaaagt
    18361 cagcgcgatt ctggcagtac gcgagttccc cgaagaccaa tacgtcttct tcgccaccgc
    18421 gcagggaatg gtgaaaaaag tccaactttc cgcctttaaa aacgtccgcg cccaaggcat
    18481 taaagccatc gcgctcaaag aaggcgacta cctcgtcggc gctgcgcaaa caggcggtgc
    18541 ggacgacatc atgctgttct ccaacttagg taaagccatc cgcttcaacg aatactggga
    18601 aaaatccggc aacgacgaag cggaagatgc cgacatcgaa accgaaattt cagacggcat
    18661 cgaagatgaa accgccgaca gcgaaaacgc actgccgagc ggcaaacacg gtgttcgccc
    18721 gtccggtcgc ggcagcggcg gtttgcgcgg tatgcgcctg cctgccgacg gcaaaatcgt
    18781 cagcctgatt accttcgccc ctgaaaccga agaaagcggt ttgcaagttt taaccgccac
    18841 cgccaacgga tacggaaaac gcaccccgat tgccgattac agccgcaaaa acaaaggcgg
    18901 gcaaggcaat attgccatta acaccggcga gcgaaacggc gatttggtcg ccgcaacctt
    18961 ggtcggcgaa accgacgatt tgatgctgat taccagcggc ggcgtactta tccgcaccaa
    19021 agtcgaacaa atccgcgaaa ccggccgcgc cgcagcaggc gtgaaactga ttaacttgga
    19081 cgaaggcgaa accttggtat cgctggaacg tgttgccgaa gacgaatccg aactctccga
    19141 cgcttctgta atttccaatg taaccgaacc ggaagtcgag aactgaaaat catctcccga
    19201 tgccgtctga agattcagac ggcatttatt ttatccctca tccgtcatcc agcttctcac
    19261 aatatagcgg attatagtca attaaaaaca aggggctgtc ctagataact agggaaattc
    19321 aaattaagtt agagttgccc ctatgagaaa aagtcgtcta agccggtata aacaaataaa
    19381 ctcattgaac tgtttgtcgc aggtgtaact gcaagaacga cagcagagtt agtaggcgtt
    19441 aataaaagta ccgcagccta ttattttcat cgtttacgat tacttattta tcaaaacagt
    19501 ccgcatttgg aaatgtttga tggcgaagta gaagcagatg aaagttattt tgctgaacga
    19561 caaaaccata tcaatggaat tgagaactt
//
LOCUS       DAWEWP010000025        19492 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWEWP010000025 DAWEWP010000000
VERSION     DAWEWP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.025"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            299..1627
                     /gene="miaB"
                     /locus_tag="WGN53_000634"
     CDS             299..1627
                     /gene="miaB"
                     /locus_tag="WGN53_000634"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ0139650.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1712..3625)
                     /gene="dxs"
                     /locus_tag="WGN53_000635"
     CDS             complement(1712..3625)
                     /gene="dxs"
                     /locus_tag="WGN53_000635"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ0139651.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3710..4627)
                     /gene="xerC"
                     /locus_tag="WGN53_000636"
     CDS             complement(3710..4627)
                     /gene="xerC"
                     /locus_tag="WGN53_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ0139652.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDEGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4821..5885
                     /locus_tag="WGN53_000637"
     CDS             4821..5885
                     /locus_tag="WGN53_000637"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ0139653.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            5905..6882
                     /locus_tag="WGN53_000638"
     CDS             5905..6882
                     /locus_tag="WGN53_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ0139654.1"
                     /translation="MPSEPPFGRHLIFASLTCLIDAVCKKRYHNQNGYILSILRMTRS
                     KPVNRTTFCCLSLTAALILTACSSGGGGSGGVAADIGTGLADALTAPLDHKDKGLKSL
                     TLEDSIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKNDKISRFDFVQKIEVDGQTI
                     TLASGEFQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPGG
                     KAEYHGKAFSSDDPNGRLHYTIDFTNKQGYGRIEHLKTPEQNVELASAELKADEKSHA
                     VILGDTRYGSEEKGTYHLALFGDRAQEIAGSATVKIGEKVHEIGIAGKQ"
     gene            7020..7697
                     /gene="tsaB"
                     /locus_tag="WGN53_000639"
     CDS             7020..7697
                     /gene="tsaB"
                     /locus_tag="WGN53_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ0139655.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETRLFHQEVGSRQSELI
                     LPEIRTLFHDAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAMDARMGEVFYAWFDTLNCRRLSDYQVGRAADIRLPEGCAFSDG
                     IGNAFALENRPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRDKIALTAKEQ
                     AERRARL"
     gene            7694..8134
                     /gene="rimI"
                     /locus_tag="WGN53_000640"
     CDS             7694..8134
                     /gene="rimI"
                     /locus_tag="WGN53_000640"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ0139656.1"
                     /translation="MNIRRAVYADCAVLAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRLAAFIVWQNLPDESELHLIATAPECRRRGIASALLEYWFANLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8128..8868
                     /locus_tag="WGN53_000641"
     CDS             8128..8868
                     /locus_tag="WGN53_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ0139657.1"
                     /translation="MLSARYLHLHEALGLGPMWLKRGAVVLPSATLPGSPTQIRPQKQ
                     TVLSIPQRPSEQHTGQARLKTMKVLETTAVHTRKPAPETETPLSGVSDGIAPVSAASG
                     ITKLAVVSLCPPTEDMVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNP
                     MPSEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPA
                     RLLRQPELKARNWQVLKQLKHALQAGGS"
     gene            8930..9571
                     /gene="pyrE"
                     /locus_tag="WGN53_000642"
     CDS             8930..9571
                     /gene="pyrE"
                     /locus_tag="WGN53_000642"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ0139658.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRQSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIQFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9645..10079
                     /locus_tag="WGN53_000643"
     CDS             9645..10079
                     /locus_tag="WGN53_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139659.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGMFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAIGKKQISRDEIADILNGGTTLHDTPPATAATP
                     AAAPQVAVPPAAPARQEGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10076..11386
                     /gene="argA"
                     /locus_tag="WGN53_000644"
     CDS             10076..11386
                     /gene="argA"
                     /locus_tag="WGN53_000644"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ0139660.1"
                     /translation="MIVPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSTVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEISCLAVSPQAQDGGYGERLLAHIIDKARSMGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11482..13497
                     /locus_tag="WGN53_000645"
     CDS             11482..13497
                     /locus_tag="WGN53_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ0139661.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNQVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGIEVRQFWVMSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
     gene            complement(13611..14567)
                     /locus_tag="WGN53_000646"
     CDS             complement(13611..14567)
                     /locus_tag="WGN53_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0139662.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRSFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            14803..15768
                     /locus_tag="WGN53_000647"
     CDS             14803..15768
                     /locus_tag="WGN53_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0139663.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
     gene            16820..17143
                     /locus_tag="WGN53_000648"
     CDS             16820..17143
                     /locus_tag="WGN53_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0139664.1"
                     /translation="MQNETINPKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            17257..19434
                     /locus_tag="WGN53_000649"
     CDS             17257..19434
                     /locus_tag="WGN53_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0139665.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDLSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT         4453 a         6034 c         5250 g         3755 t
ORIGIN      
        1 tgttaatcca ctataacgca ggaactgatg ttccctgtcg ccgaaattgc tggtacacgc
       61 acacagcagc aatgccgccc acacagccgg tttcatacac acctcccatt aaagccaaac
      121 attatacagc cgttctgacc gattaaattc atattttaaa acaatattct gtttacaaaa
      181 cccacatcgt gctataatcc gcaccgattt tcagacggca tcgtcatgcc gtctgaaatt
      241 ttttcattcc aacaaccatc agccccgcga ttacggctgc ctgagaaaga cacaaaccat
      301 gaaaaaagta tttatccgta ccttcggctg ccagatgaac gaatacgaca gcgacaaaat
      361 gctcgccgtc ctcgccgaag aacacggcgg catcgaacag gttacccaag ccgacgaagc
      421 cgacatcatc ttgttcaaca cctgctccgt gcgcgaaaaa gcacaggaaa aagtgttctc
      481 cgatttgggg cgcgtgcgtc cgctcaaaga aaaaaacccc ggcctcatca tcggcgttgc
      541 cggctgcgtc gcctcgcaag aaggcgaaaa catcatcaaa cgcgcgcctt atgttgacgt
      601 ggtcttcggc ccgcaaacgc tgcaccgcct gcccaaaatg attgtggaca aagaaaccag
      661 cgggctgtcg caagtcgata tttccttccc cgaaatcgaa aaattcgacc acctgccgcc
      721 cgcccgcgtc gaaggcggcg cggcatttgt atcgattatg gaaggctgtt ccaaatactg
      781 ctcattctgc gtcgtcccct acacgcgcgg cgaagaattc tcccgcccgc tcaacgacgt
      841 attgacagaa atcgccaacc ttgcccagca aggcgtgaaa gaaatcaacc tcttgggaca
      901 aaacgtcaac gcctatcgcg gcgaaatgga cgacggcgaa atctgcgact tcgccaccct
      961 gctgcgcatc gtccacgaaa tccccggcat cgaacgtatg cgcttcacca ccagccaccc
     1021 gcgcgagttt accgactcga ttatcgagtg ctaccgcgac ctgcccaaat tggtttccca
     1081 cctgcacctg ccgattcaaa gcggttccga ccgcgtattg agcgcgatga aacgcggcta
     1141 caccgctttg gaatacaaat ccatcatccg caaactgcgt gccatccgtc ctgatttatg
     1201 cctgagcagc gatttcatcg tcggcttccc cggcgagacc gaacgcgagt tcgagcaaac
     1261 cttgaaactg gtgaaagaca ttgccttcga cttgagcttc gtgtttattt acagtccgcg
     1321 ccccggcacg cctgccgcca acctgcacga cgacacgccg cacgaagaaa aagtgcgccg
     1381 cctcgaagcc ttgaacgaag tcatcgaagc cgaaaccgcg cgcatcaacc aaaccatgat
     1441 cggcacggtt caacgctgcc tggtcgaagg catctccaaa aaagaccccg accaactgca
     1501 agcccgtacc gccaacaacc gcgtcgtcaa cttcaccggc acgcccgaca tgattaacca
     1561 aatgatcgat ttggaaatca ccgaggccta caccttctcc ctgcgcggca aagttgtcga
     1621 agcctaaacc ctcacgccga aaaaatgccg tctgaagcgt ttcagacggc attttgcctt
     1681 gtatcggcag acgacggcgc ggccgggcgg cttaatttgc cgcatcccga tccgacagcc
     1741 acgcgcgcac acgccgttcc accgcttcgg cactcaagcc caaatcgtct aaaagttttt
     1801 tcggatcgcc gtgtccggtt acggtatcgg caacgcccaa aagcaaaacg ggtttgcaga
     1861 taccgtgttt cgccaatact tccagcaccg cgccgcctgc gccgccctgt tcggcgtttt
     1921 cttcaagggt aacgatgcgg tcgtggcttc gggcaaggcg gacaatcaac tcttcgtcta
     1981 tcggtttgac aaagcgcata tcggcgacgg tggcgttcag ttttccggcg accgccaatg
     2041 cgggggcgac catactgccg aaggcaatga atgcggtttt cccaccttcg cggcggataa
     2101 tgcccttgcc gatttccacg gtttccatgc cgtctgaaac cggcgcgccc gtacccgtgc
     2161 cgcgcggata gcggacggcg gcgggcgcat ccgcctgata gcaggtcgaa agcagcaggc
     2221 ggcattcgtt ttcatcgctc ggcgcggcga caatcatatt cggaatgcaa cgcaagaagc
     2281 tcaaatcgta caaaccggcg tgggtcgggc cgtccgcgcc gacgatgccc gcgcggtcga
     2341 cggcaaacaa aacgggtagg ttttgcaggg cgatatcgtg caccagttgg tcgtaggcgc
     2401 gttgtaaaaa ggtggaataa atcgccacga cgggcttcat gccttcgcac gccaaaccgc
     2461 cggcaaaggt aacggcgtgc tgctcggcaa taccgacatc gaaataacgg tcggggaatc
     2521 gttgttcaaa ctccaccagt ccgctgccct cgcgcatcgc gggcgtgatg gcgatcaaac
     2581 gctcgtctgc cgccgcccgg tcgcacagcc atttgccgaa cacttgggta taggtcggtt
     2641 tggcgacggg cttgggttct ttttcagacg gcatttgcgc cgcgccttct ttaggcagct
     2701 tggcgacggc gtggtatttg acggggtcgt tttcggcgag tttgtagccg ttgccttttt
     2761 tggtgatgac gtgcagaagc tgagggcctt tgcggctgcg caagtctttc aatacgtcca
     2821 ccagattttc gacgttgtgt ccgtccacgg ggccggtata gcggaagccg aagttttcaa
     2881 acaaagacag cgactgtttg gcgtgttcgg cttcttcggc aagggttttg attttgtgtt
     2941 cgactttttg ggcaaactcc atcgcgccgg gtattttgtc taataccttg cccgtttgcg
     3001 ctttgacggt actcaacagg ccgtgcatat cgcgcacgac gttgctggca aggtatttcg
     3061 gcagcgcgcc gacgttgggg gaaatcgaca tttcgttgtc gttgaggacg accagcaaat
     3121 ccacatccat atcgcctgcg cagttcaggg cttcaaacgc ctgccccgcc gtcatcgcgc
     3181 cgtcgccgat gatggcgacg ctgcggcggt cgctgcccaa gagtttgtct gccgccgcca
     3241 tgcccaacgc cgcaccgatg gaggtggagg aatgccccac gccgaacgcg tcgtactcgg
     3301 actcgcaacg tttcggaaaa cccgccaaac cgccatattg gcgcatggtg tgcatctggt
     3361 ttttcctgcc tgtcaggatt ttgtgcggat agctttggtg tccgacatcc cacaccagct
     3421 tgtcttcggg cgtatcgtac acatagtgca gggcgatggt cagttcgacc gcgcccagat
     3481 tgctggcgaa atgcccgccg gtctgcccga cagattccag cagaaaggcg cgcaactcgc
     3541 cggcaaggcg cggcagctgt tttttgtcca gacggcgcaa atcttgcggg ctgtcaatca
     3601 ggtcgagtag ggggcttggg ttcatggtgt gtctttttta tgtgtcgtcc gggtgcaacg
     3661 gccaattata tatcaagagc gtgcggctga cggctgattt tgccgtatgt cattcgtcct
     3721 gccgcttggc gcgcgggtgg gcttcgtcat acaggcgggc gatgtggtcg aaatcgagtt
     3781 tggtgtaaat ctgcgtggtc gaaaggctgc tgtgcccgag cagctcctgc accgccctga
     3841 tgtcgcgcga agcctgcaac aggtgtccgg cgtagctgtg gcgcatcata tgcggcgaaa
     3901 cgtgcctgcc gtcgccgttt tgcgccgccc attgcgccaa acgtttttgg atttggcgtt
     3961 ggctcaggcg cgtgccgttc ctgccagtaa acagggcttt gccgtccgat gccgtctgac
     4021 gcagcggcag atagtttttc agagcttcca cgcttttgcc ggtcagcgga acctgccgct
     4081 gcttgcgtcc tttgccggta acgtgtaccc acgcctcgtc caaatataca tcatctgcat
     4141 tcaagccgtg tatctcgctc acgcgcaaac cgctgccgta catcagttcg aacagcgcgt
     4201 gatcgcgcac cgccagcggg tcgccgccgt ccacgggcaa atccagcatc cggttcagcc
     4261 attcctgcgg cagggctttg ggtacgcgct cgggctgctt cggcggtttg atgtcggcgg
     4321 tcgggtcggt atgcagcaga ccgcgttcca ccagccaaac acaatactgc cgccaagacg
     4381 acagcttgcg cgccagcgtc cgcgcattca aaccgcgctg cgacagccgc cgcaacgccg
     4441 ccgtaaaatc gcgccgcgac aagtcctgcg gcacgccgcc ttcatcttca gacggcattt
     4501 gtgccaacag cgcaaacagt tcttccaaat cgcgccggta tgccgcaacc gtgtgttccg
     4561 acttgccctc gcgcacgatg ttttccaaat aagcgtcaaa atgcgccgca aacccgtcca
     4621 aaaccataac cgtcccacac aaatatcaaa aaaccagtga aaacattatc ataaacgaca
     4681 caccaaaacc ccgaaccgcc caaaatccga cccgtccgcc cgccgttatc gttaacataa
     4741 aatcaatgag ccgtttatgg ttttttgccg caaaaaacat tataatccgc cttatttacc
     4801 tattgcccaa ggagacacaa atggcactcg tatccatgcg ccaactgctt gatcatgctg
     4861 ccgaaaacag ctacggcctg cccgcgttca acgtcaacaa cctcgaacaa atgcgcgcca
     4921 ttatggaagc cgccgaccaa gtcaacgcgc ccgtcatcgt acaggcgagc gcaggtgcgc
     4981 gcaaatatgc gggcgcgccg tttttgcgcc acctgattct ggcggcagtc gaagaatttc
     5041 cgcacatccc cgtcgtgatg caccaagacc acggcgcatc gcccgacgtg tgccaacgct
     5101 ccatccaact gggcttctcc tccgtgatga tggacggctc gctgatggaa gacggcaaaa
     5161 ccccttcttc ttatgaatac aacgtcaacg ccacccgtac cgtggttaac ttctcccacg
     5221 cttgcggcgt atccgtcgaa ggcgaaatcg gcgtattggg caacctcgaa accggcgaag
     5281 caggcgaaga agacggtgta ggcgcagtgg gcaaactttc ccacgaccaa atgcttacca
     5341 gcgtcgaaga tgccgtgcgt ttcgttaaag ataccggcgt tgacgcattg gcgattgccg
     5401 tcggcaccag ccacggcgcg tacaaattca cccgtccgcc cacaggcgac gtgttgcgta
     5461 tcgaccgcat caaagaaatc caccaagccc tgcccaatac acacatcgtg atgcacggct
     5521 ccagctccgt tccgcaagaa tggctgaaag tcatcaacga atacggcggc aatatcggcg
     5581 aaacctacgg cgtgccggtt gaagaaatcg tcgaaggcat caaacacggc gtgcgtaaag
     5641 tcaacatcga taccgacttg cgccttgctt ccaccggcgc ggtacgccgc taccttgccg
     5701 aaaacccgtc cgacttcgat ccgcgcaaat atttgagcaa aaccattgaa gcgatgaagc
     5761 aaatctgcct cgaccgctac ctcgcgttcg gttgcgaagg tcaggcaggc aaaatcaaac
     5821 cggtttcctt ggaaaaaatg gcaagccgtt atgccaaggg cgaattgaac caaatcgtca
     5881 aataacaggt tgcttgtaaa caaaatgccg tctgaaccgc cgttcggacg gcatttgatt
     5941 tttgcatctt tgacctgcct cattgatgca gtatgcaaaa aaagatacca taaccaaaat
     6001 ggttatatat tatctattct gcgtatgact aggagcaaac ctgtgaaccg aactaccttc
     6061 tgctgccttt ccctgaccgc cgccctgatt ctgaccgcct gcagcagcgg aggcggcgga
     6121 agcggaggtg tcgccgccga catcggcacg gggcttgccg atgcattaac cgcgccgctc
     6181 gaccataaag acaaaggttt gaaatccctg acattggaag actccattcc ccaaaacgga
     6241 acactgacct tgtcggcaca aggtgcggaa aaaactttca aagccggcgg taaagacaac
     6301 agcctcaaca cgggcaaact gaagaacgac aaaatcagcc gcttcgactt cgtgcaaaaa
     6361 atcgaagtgg acggacaaac catcacgctg gcaagcggcg aatttcaaat atacaaacag
     6421 gaccactccg ccgtcgttgc cctacagatt gaaaaaatca acaaccccga caaaatcgac
     6481 agcctgataa accaacgctc cttccttgtc agcggtttgg gcggagaaca taccgccttc
     6541 aaccaactgc ccggcggcaa agccgagtat cacggcaaag cattcagctc cgacgacccg
     6601 aacggcaggc tgcactacac catcgatttt accaacaaac agggttacgg cagaatcgaa
     6661 cacctgaaaa cacccgagca aaatgtcgag cttgcctccg ccgaactcaa agcagatgaa
     6721 aaatcacacg ccgtcatttt gggcgacacg cgctacggca gcgaagaaaa aggcacttac
     6781 cacctcgccc ttttcggcga ccgcgcccaa gaaatcgccg gctcggcaac cgtgaagata
     6841 ggggaaaagg ttcacgaaat cggcatcgcc ggcaaacagt agcatttgaa aaataccgtc
     6901 cgaacacgat agtttaccgt tcggacggca tttttgcgcc atactgccct aggatacgac
     6961 ggcacggcag gcaagccgat ttcactatat aatgccgtct gaaccaacga gagaatgcca
     7021 tgcaagctga ttttaaccgt cccgtcctgg ccgtcgatac cggaacttcc tatttgtcgc
     7081 tcgcgctgcg cgccgacggc gaaacccgcc tgtttcatca ggaagtcggc agccgccagt
     7141 ccgaactgat tctgccggaa atccgcacgt tgtttcacga tgcaggcatt accgccgccg
     7201 atttgggggc gattgtttat gcgaaaggcc ctggcgcgtt tactggactg cgtatcggca
     7261 ttggcgtggc gcaaggtttg gcaacgccgt ttgatacccc tttaatcggc atccccacgc
     7321 tcgatgccgc cgcctcgctg ccgccgccgc aaagctgcat ccttgccgct atggacgctc
     7381 gtatgggcga agtgttttat gcatggttcg atacgctgaa ctgccgccgt ttgagcgatt
     7441 atcaggtcgg tcgggcggca gacatccggc tgccggaagg atgcgccttt tcagacggca
     7501 tcggcaatgc gttcgcgctg gaaaaccgtc cgcccttctc aggcaaaccc gatatgccga
     7561 ccgctgccga ctttctcaat ctggcggcgg aaggcggttt tcccgccgtc gctgcggcac
     7621 acgccgagct gctctacgtc cgcgacaaaa tcgccctgac tgccaaagaa caggcggaac
     7681 ggagggcgcg cctgtgaaca tccgccgtgc cgtttatgcc gattgcgcgg tactcgccgc
     7741 actcgatgcc gtctgcaacc cgtccgcgtg gacgcaacgc caatttgagt ccgcactggt
     7801 ttcgccgtcc gaacaggttt tccttgcgga aaaagacggc aggttggccg cctttatcgt
     7861 ttggcagaac ctgcccgacg aatccgaact gcacctgatt gccaccgcgc ccgaatgccg
     7921 ccgccgtggc attgcgtccg ccctactcga atactggttc gccaacctgc ccgaaggcac
     7981 gcaacgcctg ctgctcgaag tccgcgcggg caacaccgcc gcacaggcac tgtacgcggc
     8041 gcacggcttt accgatgcgg gcaggcggaa aaactattac cgtacagccg acggtaaaac
     8101 cgaagatgcc gtcttaatgg agaaaatatg ttaagcgcgc gctacctcca cctgcacgaa
     8161 gccttgggtt tgggtccgat gtggttgaaa cggggagccg tcgttctgcc gtcagcaaca
     8221 ttgccgggaa gcccgacaca aatccgcccg caaaagcaaa ccgtcctcag cattccgcag
     8281 cgtccgtccg aacagcatac cggtcaggca cggctcaaaa ccatgaaagt gttggaaaca
     8341 accgccgtac atacgcgcaa acccgcgcct gaaaccgaaa cgcctctgtc cggcgtttca
     8401 gacggcatcg cccccgtttc cgccgcttcg ggcataacca aacttgccgt cgtcagcctg
     8461 tgtccgccga ccgaggatat ggtttacggg caactgttcc acggcaaagc gggtgtcctg
     8521 cttgacaaca tactcaaagc cgtaggactg gatgccgctt atgtacacaa gaccagctgg
     8581 gtgaaaaccg ccgccgtcgg caacccgatg ccgtctgaac aggccgtcgc gaatgcgctg
     8641 ggtcaaatcg ccagggaact cgacggctgc cgcgccccgg ccgtcctttt cctcgggcag
     8701 gcttttgtcc agcctgaacg gcaaacgatg attgaaactt tgtgcggcag ccgtcccttc
     8761 ttcatcatcg accatcccgc ccggctttta cgccaacccg aacttaaagc ccgaaactgg
     8821 caggtgttga aacagttgaa acacgccttg caggcaggcg gcagttgaag caggccgcac
     8881 ggggcggtag aatcacaact gcatcgcaat acctgacaga aagcaaaaaa tgaccgattt
     8941 ccgccaagat ttccttaaat tctccctcgc ccaaaacgtc ttaaaattcg gcgaatttac
     9001 caccaaggca ggacggcagt cgccttactt cttcaatgcc ggcctcttta acgacggctt
     9061 gtccacgctg caactggcaa aattctatgc acaatccatc attgaaagcg gcatccaatt
     9121 cgatatgctg ttcggccccg cctacaaagg cattattttg gcggcggcaa ccgcgatgat
     9181 gctggcggaa aaaggcgtaa acgtcccgtt tgcctacaac cgcaaagaag ccaaagacca
     9241 cggcgaaggc ggcgtgttgg tcggcgcgcc gcttaaaggg cgcgtgctga ttatcgacga
     9301 tgtgatttcc gccggcacat ccgtacgcga atcgatcaaa ctaattgaag cggagggtgc
     9361 aacccccgcc ggcgtcgcca tcgcgctcga ccgcatggaa aaaggcaccg gcgaattgag
     9421 cgcggttcag gaagtggaaa aacaatacgg cctgcccgtc gcccccatcg ccagcctgaa
     9481 cgatttgttt atcctgttgc aaaacaaccc cgaattcgga cagttcctcg aacccgtccg
     9541 agcctaccgt cggcagtacg gcgtagaata aaaacaaagc atatgccgtc tgaaccacct
     9601 tacgcctcag acggcatcaa acctgacaca cacgaggaaa taccatgcct gcctgtttct
     9661 gtccgcactg caaaacccgc ctctgggtca aagaaaccca actcaatgtc gcccaaggct
     9721 tcgtcgtctg ccaaaaatgc gaagggatgt ttaaagccaa agaccatttg gcaagcacga
     9781 aagaacctat attcaacgat ttgcccgagg ctgtttcgga tgtcaaactc gttcaccgca
     9841 tcggcacgca cgccattggc aagaaacaga tttcccgcga cgaaatcgcc gatatcctca
     9901 acggcggtac aaccctgcac gatacgccgc ccgcaaccgc cgccacccct gccgccgcac
     9961 cgcaggttgc cgtaccgccc gccgcgcctg cccgtcagga agggttcaac tggacgattg
    10021 caaccctgtt tgcccttatc gtcctcatta tgcagctttc ctacctcgtc atcctatgat
    10081 cgtgcccgac ctctttgtcg cccacttccg cgaagccgtc ccctacatcc gccaaatgcg
    10141 cggcaaaacg ctggtcgccg gcatagacga ccgcctgctc gaaggtgata ccttaaacaa
    10201 gctcgccgcc gacatcgggc tgttgtcgca actgggcatc aggctcgtcc tcatccacgg
    10261 cgcgcgccac ttcctcgacc gccacgccgc cgcgcaaggc cgcacgccgc attattgccg
    10321 gggcttgcgc gttaccgacg aaacctcgct cgaacaggcg cagcagtttg ccggcaccgt
    10381 ccgcagccgt tttgaagccg cattgtgcgg cagcgtttcc gggttcgcgc gcgcgccttc
    10441 cgtcccgctc gtatcgggca acttcctgac cgcccgtccg ataggtgtga ttgacggaac
    10501 cgatatggaa tacgcgggcg ttatccgcaa aaccgacacc gccgccctcc gtttccaact
    10561 cgacgcgggc aatatcgtct ggctgccgcc gctcggacat tcctacagcg gcaagacctt
    10621 ctatctcgat atgcttcaaa ccgccgcctc cgtcgccgtc tcgcttcagg ccgaaaaact
    10681 cgtttacctg accctttcag acggcatttc ccgccccgac ggcacgctcg ccgaaaccct
    10741 ctcggcacag gaagcgcaat cgctggcgga acacgccggc ggcgaaacgc gacggctgat
    10801 ttcgtccacc gttgccgcgc tcgaaggcgg cgtgcatcgc gtccaaatcc tcaacggtgc
    10861 ctccgacggc agcctgctgc aagaactctt cacccgcaac ggcatcggca catccattgc
    10921 caaagaagcc ttcgtctcca tccggcaggc gcacagcggc gacatcccgc acatcgccgc
    10981 cctcatccgc ccgctggaag aacagggcat cctgctgcac cgcagccgcg aatacctcga
    11041 aaaccacatt tccgaatttt ccatcctcga acacgacggc aacctgtacg gttgcgccgc
    11101 cctgaaaacc tttgccgaag ccgattgcgg cgaaatctcc tgccttgccg tctcgccgca
    11161 ggcacaggac ggcggctacg gcgaacgcct gcttgcccac attatcgata aggcgcgcag
    11221 tatgggcata agcaggctgt tcgccctctc cacaaatacc ggcgaatggt ttgccgaacg
    11281 cggctttcag acggcatcgg aagacgagtt gcccgaaacg cggcgcaaag actaccgcag
    11341 caacggacga aactcgcata ttctggtacg tcgcctgcac cgctgaccgc aacggaaagc
    11401 cgccgcagaa atgccgtctg aaccccgttt cagacggcat ttccccgatt ataaagacct
    11461 gcccaaccct caaggacccc gatgaaatcc taccccgacc cctaccgcca ttttgaaaac
    11521 ctcgattccg ccgaaacgca aaacttcgct gctgaagcga atgccgaaac gcgcgcgcgt
    11581 tttttaaaca acgacaaggc gcgcgcgctt tcagacggca ttttgaatca aatgcaggac
    11641 acgcggcaga ttccgttttg tcaggaacac cgcgcgcgga tgtaccattt ccatcaggac
    11701 gcggaatatc cgaagggcgt gtaccgcgtg tgcacggcgg cgacctaccg ttcgggctat
    11761 ccggagtgga aaatcctgtt ttcagtggcg gacttcgacg aattgctcgg cgacgatgta
    11821 tatttgggcg gcgtgtcgca cttggtggaa aagcccaacc gcgcgttgtt gacactaagc
    11881 aaatcaggcg gcgatacggc gtacacgctg gaagtggatt tggaagcagg ggaattggta
    11941 gaaggtggtt ttcactttcc ggcaggcaaa aaccaagtgt cgtggcgcga tgaaaacagc
    12001 gtgtgggtgt gtccggcttg ggacgaacgc cagttgaccg aatcgggcta tccgcgtgag
    12061 gtgtggctgg tggaacgcgg caagagtttc gaggaaagcc tgcctgtgta tcaaatcgat
    12121 aaagatgcaa tgatggtgaa cgcgtggcgt tatctcgatc cgcagggttc gccgattgat
    12181 ttgattgaag cgtcggacgg tttttacacc aaaacctatt tgcaggtgtc ggccgaaggc
    12241 gaggcgaaac cgttaaacct gcccaacgat tgcgatgtgg tcggctatct ggcggggcat
    12301 cttttgctga cgctgcgcaa ggactggaac cgcgcgaacc aaagctatcc gagcggtgcg
    12361 ctggtggcgg tgaagctgaa tcggggtgaa ctcggggcgg cgcagctttt gtttgcgcct
    12421 gatgaaacgc aggcattgga aagcgtggaa acgaccaagc gttttgtggt ggcgagcctg
    12481 ctggaaaacg tacaaggccg tctgaaagcg tggcgttttg ccgacggcaa atggcaggaa
    12541 gtcgaactgc cgcgcctgcc ttcgggcgcg ttggaaatga ccgaccagcc ttggggcggc
    12601 gacgtggttt accttgccgc cggcgatttc accacaccgc tgacgctgtt tgcgctggat
    12661 ttgaacgtga tggaactgac cgtcatgcgc cgccagccgc agcagttcga ttcagacggc
    12721 atcgaagtgc ggcagttttg ggtgatgtcg tccgacggcg aacgcatccc ttatttccac
    12781 gtcggcaaaa acgccgcgcc cgacacgccg acgctggttt atgcctacgg cggtttcggc
    12841 gtacccgagc tgccgcatta tctgggcagc atcggcaaat actggctgga agagggcaac
    12901 gccttcgtgt tggcgaacat acgcggcggc ggcgagttcg gcccgcgctg gcatcaggcg
    12961 gcgcagggaa tcagcaaaca caaaagcgtc gatgatttgc ttgccgtcgt gcgcgatttg
    13021 tccgagcgcg gcatcagttc gccccaacac atcggtttgc agggcggcag caacggcggc
    13081 ctgattaccg ccgccgcctt cgtgcgcgaa ccgcaaagca taggcgcact ggtgtgcgaa
    13141 gtgccgctga ccgacatgat ccgttatccg ctgctctccg ccggttcaag ctggacagac
    13201 gaatacggca atccgcaaaa atacgaagtc tgcaaacgcc ggttgggcga attgtcgccg
    13261 tatcacaatc tttcagacgg catcgattat ccgcccgcgc tcatcaccac cagcctcagc
    13321 gacgaccgcg tccatcccgc ccacgcgctc aagttttacg ccaaactgcg cgaaacctcg
    13381 ccgcaatctt ggctctactc gcctgacggc ggcggccata ccggcaacgg cacgcagcgc
    13441 gaagccgccg acgaactcgc ctgcgtgttg ctgtttttga aagagttttt gggctaaggg
    13501 cggggagcgg cactgccgcc gcgaatgaaa aaaggtcgtc tgaaactgct ttttcagacg
    13561 acctttttta atggttgttt caaatcaaaa tatctatgcc gccggcccca tcagcacttc
    13621 ttcacatccg aaggcaaaaa tccgtaatgc cgtctgaacg cttcgttgaa ccgtcccgcg
    13681 tggcggtagc cgcaaaagtg catggcggct tggacggtgc tgccggattc gatgagggcg
    13741 agcgcgtgtt ccagccgcag gcggcgcagg catccggcga cggtttcgcc ggtttgcgct
    13801 ttgaaatagc gtttcaggta gcattcgttc agtccgacgc ggcgggcgat ttcggcgatg
    13861 gtcagcggac gggcgaattc gtgttgcagg atgtcggcgg cttcgtctat gcgccgacgg
    13921 cggtaaccgt tgtcgtggcg gcggaaggtg aagcgcaata atcgggcgga gagttccagc
    13981 gcggcggctt cgtcggcaag caggccgaag ccgtccgatt cgaacgagcg ttgcagcagt
    14041 gggcaggccg ccgccgtcag tgccgctgcg ttttgcgcca gccgttgcag ggcgaatcgg
    14101 cctatcgttt gcggcgaaaa caggcgttcg tccagcaagc cttcgtcgtg ccagcggcgc
    14161 agtttttcca gcgaaaaatc caaatgcagc gcgcacatgc cgctgttgtc gggcagcagg
    14221 gtttcggata cgtccgccaa ctcgccgcgt accagccaga tttcgccggc agacgggcgg
    14281 tattccctgc cgcccatttg taaccggttc tgccccgaca ccatgacgaa caaggcgcag
    14341 ttgtggctga aattgtggat ttcggtggga aacgcgcccg ttccgccgcc gcgcatccgc
    14401 gacaaggtga tgcccgagtc gaagcggttg atgcacattt ccagatgcaa accgggctgt
    14461 tttgcctgcg caatgagcgc gctgtcggaa cagccgtcca acgcccagcc ggatttatcg
    14521 gagcggacat aggtttggta ctggcggtag atggcggcgg tgttcatgat tggataggaa
    14581 cgagttgtct aacaaatgaa ttaaatagga attattacca ataatcaagc gcagggattg
    14641 gttgaaacgg aaaaggtcgt ctgaaagggt gtttcagacg accttttccg tatcgggaat
    14701 ttgttttgcc gtatcgggaa ttttgcgttt tgcggcgtgg tttctgcagg ttgtttgctt
    14761 aataataaac attcttattc gtatgcaaag gaaccgcaca ccgtgaaacc gcgtttttat
    14821 tgggcagcct gcgccgtcct gctgaccgcc tgttcgcccg aacctgccgc cgaaaaaact
    14881 gtatccgccg catccgcatc tgccgccaca ctgaccgtgc cgaccgcgcg gggcgatgcc
    14941 gttgtgccga agaatcccga acgcgtcgcc gtgtacgact gggcggcgtt ggatacgctg
    15001 accgaattgg gcgtgaatgt gggcgcaacc accgcgccgg tgcgcgtgga ttatttgcag
    15061 cctgcatttg acaaggcggc aacggtgggg acgctgttcg agcccgatta cgaagccctg
    15121 caccgctaca atcctcagct tgtcattacc ggcgggccgg gcgcggaagc atatgaacag
    15181 ttggcgaaaa acgcgaccac catagatctg acggtggaca acggcaatat ccgcaccagc
    15241 ggcgaaaagc agatggagac cttggcgcgg attttcggca aggaagcgcg cgcggcggaa
    15301 ttgaaggcgc agattgacgc gctgttcgcc caaacgcgcg aagccgccaa aggcaaagga
    15361 cgcgggctgg tgctgtcggt tacgggtaac aaggtgtccg ccttcggcac gcagtcgcgg
    15421 ttggcaagtt ggatacacgg cgacatcggc ctaccgcctg tagacgaatc tttacgcaac
    15481 gaggggcacg ggcagcctgt ttccttcgaa tacatcaaag agaaaaaccc cgattggatt
    15541 ttcatcatcg accgtaccgc cgccatcggg caggaagggc cggcggctgt cgaagtattg
    15601 gataacgcgc tggtacgcgg cacgaacgct tggaagcgca agcaaatcat cgtcatgcct
    15661 gccgcgaact acattgtcgc gggcggctcg cggcagttga ttcaggcggc ggagcagttg
    15721 aaggaggcgt ttgaaaaggc agaacccgtt gcggcgggga aagagtaggg agtcgtctga
    15781 aaacggagtt tccgaaggaa gcggggggtt tctgcgaagc taaagtgcgg tttcaacgaa
    15841 ttgaaaagca gcctgtatgt tgaaaatacc gctcaagcaa acctacggtt tgccgccctc
    15901 tccctagccc tctcccacag ggagagggga ttggggtgca ggctgccttt aaggtttagg
    15961 caaattttta acttcgttga agctgcgatt tcagaagctc cgttttagct tcgcagaaac
    16021 tccgcttcct tcgaaagctc cgttttcaga cgaccttttg gagtaccgca ggcacacgca
    16081 tcgaacggct gaatcaaaga ttcagaccga tggcagtccg cacccgagtt tatgcggcaa
    16141 acagcgaggc tacggcaacc cgtcccctct ccctgtggga gagggttagg gagagggcag
    16201 taagccgtag gcttacatca aagccgataa cgctttcaca aagcctgtgt gcactgaaaa
    16261 ctgaaaggtc gtctgaaaac ggagctttcg aaggaagcag agtttctgcg aagctaaaac
    16321 cgaagctgca aaaaatcgaa aagcagcctg cacgttgaaa atgccgccca agcaaacctg
    16381 cggtttgccg ccctctccct agccctctcc cacagggaga ggggattggg gtgcaggctg
    16441 tctttaaggt tcaggcaaat ttttaacttc gttgaaactg cgatttcaga agctccgttt
    16501 tagcttcgca gaaactccgc ttccttcgaa agctccgttt tcagacgacc ttttggaaca
    16561 ccacaggcac acgcatcgaa cggctgaatc aaagattcag accgatggca gtccgcaccc
    16621 gagtttatgc ggcaaacagc gaggctacgg caacccgtcc cctctccctg tgggagaggg
    16681 ttagggagag ggcagtaagc cgcaggctta catcaaagcc gataacgctt ccgttacaac
    16741 tccgcccact gaaagcagcc tgcaacgaag ccaaaacgac aaaccgcatc gtaaaccacc
    16801 caacccatag gagaacccca tgcaaaacga aaccatcaac ccgaaacagc accttgccgc
    16861 catcaaagaa tactggcagc ccgaaatcat caaccgccac gggttccaat tccgcttggt
    16921 caaacttttg ggcgattacg gctggcatac gcacggatac agcgacaaag tgctgtttgc
    16981 cgtggagggc gacatggcgg tggacttcgc cgacggcggc agcatgacga tacgcgaggg
    17041 cgagatggcg gtcgtgccga agtcggtgtc gcaccgcccg cgttcggaaa acggctgctc
    17101 gttggtgctg attgagttgt ccgacccgtc cgaggccgtc tgaaaacgaa gtttccgaag
    17161 gaagctgagt ttctgcgaag ctaaaagcag cctgcacctt caatcaatat gccgaaaata
    17221 caaccccacc gcacaccaac acacaaagga aatcccatga cacgcttcaa atattccctg
    17281 ctgtttgccg ccctgttgcc cgtgtacgcg caggccgatg tttctgtttc agacgacccc
    17341 aaaccgcagg aaagcactga attgccgacc atcaccgtta ccgccgaccg caccgcgagt
    17401 tccaacgacg gctacactgt ttccggcacg cacaccccgc tcgggctgcc catgaccctg
    17461 cgcgaaatcc cgcagagcgt cagcgtcatc acatcgcaac aaatgcgcga ccaaaacatc
    17521 aaaacgctcg accgcgccct gttgcaggcg accggcacca gccgccagat ttacggctcc
    17581 gaccgcgcgg gctacaacta cctgttcgcg cgcggcagcc gcatcgccaa ctaccaaatc
    17641 aacggcatcc ccgttgccga cgcgctggcc gatacgggca atgccaacac cgccgcctat
    17701 gagcgcgtag aagtcgtgcg cggcgtggcg gggctgctgg acggcacggg cgagccttcc
    17761 gccaccgtca atctggtgcg caaacgcccg acccgcaagc cattgtttga agtccgcgcc
    17821 gaagcgggca accgcaaaca tttcgggctg ggcgcggacg tatcgggcag cctgaacacc
    17881 gaaggcacgc tgcgcggccg cctggtttcc accttcggac gcggcgactc gtggcggcag
    17941 cgcgaacgca gccgcgatgc cgaactctac ggcattttgg aatacgacat cgcaccgcaa
    18001 acccgcgtcc acgcaggcat ggactaccag caggcgaaag aaaccgccga cgcgccgctc
    18061 agctacgccg tgtacgacag ccaaggttat gccaccgcct tcggcccgaa agacaacccc
    18121 gccacaaatt gggcgaacag ccgccaccgt gcgctcaacc tgtttgccgg catcgaacac
    18181 cgcttcaacc aagactggaa actcaaagcc gaatacgact acacccgcag ccgcttccgc
    18241 cagccctacg gcgtagcagg cgtgctttcc atcgaccaca acaccgccgc caccgacctg
    18301 attcccggtt attggcacgc cgacccgcgc acccacagcg ccagcgtgtc attgatcggc
    18361 aaataccgcc tgttcggccg cgaacacgat ttaatcgcgg gtatcaacgg ttacaaatac
    18421 gccagcaaca aatacggcga acgcagcatc atccccaacg ccattcccaa cgcctacgaa
    18481 ttttcccgca cgggcgccta tccgcagcct gcatcgtttg cccaaaccat cccgcaatac
    18541 ggcaccaggc ggcaaatcgg cggctatctc gccacccgtt tccgcgccgc cgacaacctt
    18601 tcgttgatac tcggcggcag atacagccgt taccgcaccg gcagctacga cagccgcaca
    18661 caaggcatga cctatgtatc cgccaaccgt ttcaccccct acacaggcat cgtgttcgac
    18721 ctgaccggca acctgtcgct ttacggctcg tacagcagcc tgttcgtccc gcaatcgcaa
    18781 aaagacgaac acggcagcta cctgaaaccc gtaaccggca acaatctgga agccggcatc
    18841 aaaggcgaat ggcttgaagg ccgtctgaac gcatccgccg ccgtgtaccg cgcccgtaaa
    18901 aacaacctcg ccaccgcagc aggacgcgac ctgagcggca acacctacta ccgcgccgcc
    18961 aaccaagcca aaacccacgg ctgggaaatc gaagtcggcg gccgcatcac tcccgaatgg
    19021 cagatacagg caggttacag ccaaagcaaa acccgcgacc aagacggcag ccgcctgaac
    19081 cccgacagcg tacccgaacg cagcttcaaa ctcttcactg cctaccactt tgcccccgaa
    19141 gcccccagcg gctggaccat cggcgcaggc gtgcgctggc agagcgaaac ccacaccgac
    19201 cctgccacgc tccgcatccc caaccccgcc gccaaagccc gcgccgccga caacagccgc
    19261 caaaaagcct acgccgtcgc cgacatcatg gcgcgttacc gcttcaatcc gcgcgccgaa
    19321 ctgtcgctga acgtggacaa cctgttcaac aaacactacc gcacccagcc tgaccgccac
    19381 agttacggcg cactgcggac agtgaacgcg gcgtttacct atcggtttaa ataaggtcgt
    19441 ctgaaaacgg agtttctgcg aagctatagt ggattaacaa aaaccagtac gg
//
LOCUS       DAWEWP010000026        19203 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWEWP010000026 DAWEWP010000000
VERSION     DAWEWP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19203
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.026"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(239..2014)
                     /gene="ptsP"
                     /locus_tag="WGN53_000650"
     CDS             complement(239..2014)
                     /gene="ptsP"
                     /locus_tag="WGN53_000650"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ0139666.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYNAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRILLGMGLRRFSMNPNNILPVKNIILHSNIAQLENEIAKIVRCEDE
                     EKAEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(2014..2283)
                     /locus_tag="WGN53_000651"
     CDS             complement(2014..2283)
                     /locus_tag="WGN53_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ0139667.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2352..2789)
                     /locus_tag="WGN53_000652"
     CDS             complement(2352..2789)
                     /locus_tag="WGN53_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ0139668.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2870..3433)
                     /locus_tag="WGN53_000653"
     CDS             complement(2870..3433)
                     /locus_tag="WGN53_000653"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ0139669.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3502..4326)
                     /locus_tag="WGN53_000654"
     CDS             complement(3502..4326)
                     /locus_tag="WGN53_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ0139670.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLVSRQGYVFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     ASSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRYGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRVRTDR"
     gene            4418..5050
                     /locus_tag="WGN53_000655"
     CDS             4418..5050
                     /locus_tag="WGN53_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ0139671.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5257..7041)
                     /gene="rmuC"
                     /locus_tag="WGN53_000656"
     CDS             complement(5257..7041)
                     /gene="rmuC"
                     /locus_tag="WGN53_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ0139672.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQHDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERSNETTALSESLEYAAEDEAV"
     gene            complement(7380..8168)
                     /locus_tag="WGN53_000657"
     CDS             complement(7380..8168)
                     /locus_tag="WGN53_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ0139673.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8183..9532)
                     /locus_tag="WGN53_000658"
     CDS             complement(8183..9532)
                     /locus_tag="WGN53_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ0139674.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9551..10132)
                     /gene="petA"
                     /locus_tag="WGN53_000659"
     CDS             complement(9551..10132)
                     /gene="petA"
                     /locus_tag="WGN53_000659"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ0139675.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10255..11004)
                     /locus_tag="WGN53_000660"
     CDS             complement(10255..11004)
                     /locus_tag="WGN53_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ0139676.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11135..12064
                     /locus_tag="WGN53_000661"
     CDS             11135..12064
                     /locus_tag="WGN53_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0139677.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHDLLPQVTDAERDLARIIEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPIWPQIELDIVSGFQADPIGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKTVWEAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(12320..12712)
                     /gene="rpsI"
                     /locus_tag="WGN53_000662"
     CDS             complement(12320..12712)
                     /gene="rpsI"
                     /locus_tag="WGN53_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ0139678.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12722..13153)
                     /gene="rplM"
                     /locus_tag="WGN53_000663"
     CDS             complement(12722..13153)
                     /gene="rplM"
                     /locus_tag="WGN53_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ0139679.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13331..13510)
                     /gene="ssrS"
                     /locus_tag="WGN53_000664"
     ncRNA           complement(13331..13510)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WGN53_000664"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13516..13821)
                     /locus_tag="WGN53_000665"
     CDS             complement(13516..13821)
                     /locus_tag="WGN53_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ0139680.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13832..14161)
                     /locus_tag="WGN53_000666"
     CDS             complement(13832..14161)
                     /locus_tag="WGN53_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139681.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14216..15205)
                     /locus_tag="WGN53_000667"
     CDS             complement(14216..15205)
                     /locus_tag="WGN53_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ0139682.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15328..18030)
                     /gene="ppc"
                     /locus_tag="WGN53_000668"
     CDS             complement(15328..18030)
                     /gene="ppc"
                     /locus_tag="WGN53_000668"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ0139683.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEELARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAVLLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGNPDTLTALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKIIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18073..18966
                     /locus_tag="WGN53_000669"
     CDS             18073..18966
                     /locus_tag="WGN53_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ0139684.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGCTQAAEALKILLDVGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGTER"
BASE COUNT         4135 a         5219 c         5092 g         4757 t
ORIGIN      
        1 aaagcgggaa tctagaacgt aaaatctaaa gaaaccgttt tacccgacaa gtttccgtgc
       61 cgacagacct agattcccgc ctgcgcggga atgacggatt ttaggtttct gattttggtt
      121 ttctgttttt gagggaatgg ccgattttgg gtttctgttt cggttttcta ttttgcagga
      181 atggcaaaat ttcagattgc gggcattgtt aagtatttct attttttacc tgccgtattt
      241 atttccgccc cttgaagtcg gcttcttcct cgacagacac gctgttcatc tgtttgatca
      301 gcttttccgc cttttcctcg tcctcgcagc ggacgatttt ggcaatctcg ttttcgagct
      361 gtgcgatatt gctgtgcagg atgatgtttt tgacgggcag gatgttgttc ggattcatgg
      421 aaaaacggcg cagccccata ccaagcagga tgcgggtaaa cgccgtatcg cccgccatct
      481 cgccgcatac ggacacgtct ttgtccatgc ggttggcggt gcggatgacg tgttgcagca
      541 ttttcagcac ggcgggatgt ccgggctggt aaagatggct gacgctgtcg tcgccgcgat
      601 cgacggacaa gatgtattga atcaggtcgt tggtgccgac ggagatgaaa tcgaccagtt
      661 tcaaaatact gccgacggtc agcgcggcag acggaatttc aatcatacag ccgatgccga
      721 ctttaccgaa ggcatcgccg cgttcggcaa gctggcgttg cgcggtgtcg aggtggatga
      781 ggcactggcg cacttcggat acggaggtaa tcatcggcca catcatgcgg acgggaccgt
      841 gtaccgccgc acggaggatg gcgcgcatct gggtgcggaa catgaccggt tcggcaaggc
      901 acaggcggat gccggtcatg cccagcgcgg ggttgaggct gccgttgggc gtgctgtttt
      961 tcccgaacca gcgcgggttt ttgtccactc ccaaatcgac tgtccgtatc gttacgcttt
     1021 tgcccttcat ttttttgaca atcgcgttgt acacttcgta ctgctcgtct tcagacggca
     1081 tcgtatcgcg gttgaggtaa agaaactcgc tgcggaacag cccgatgccg tctgcgccga
     1141 ggttgtgcag cggtttcacg tcttcggcgg attctatatt gcccacaagc tcgatgcaga
     1201 ccccgtcggc ggtggcggcg gcggtttttt tgagcttgtt caaatcgcgt ttgtggctgc
     1261 ggtattcgcg ggcacggcgg cggtattcgt tcaacaccga ctcatccggc gcgataatca
     1321 acacgccgtt gataccgtcc acaatgaccg tttcgccttc ggtaatcagt ttgcgcgcgt
     1381 tgtgcagccc gacgacggac gggatgtcca agctcctgcc caaaatcgcc gtatgcccgg
     1441 tggggccgcc ggcatcggta acgaaggcgg caatgcgctg ctctttaaac aaaaccgtgt
     1501 cggcgggcga aaggtcgttt gcaatcagaa cggtttcgtc aaacaggttg tcggcaactt
     1561 ccaactcgtt gccctgcccg atcaggttgt tgtggatgcg gcggacgact tgcagcatat
     1621 cctgcttgcg ttcgcgcaaa taggcatcgt ccatattgtc gaattgggcg gcgagtttgt
     1681 cgctctgctg cttcaatgcc cactcggcgt tgattttttg ttcccttaaa atatcgacgg
     1741 gttcgcgcga caaggtaaca tcggtcaaga gcatcaggtg cagcgagatg aacgcgccca
     1801 actcggtcgg ggcgttttcg ggaatcgcgc tgcggagctg ttccaactct ttgcgcgtgg
     1861 ctttgacggc ggcatcgaaa cgttcggctt cggcatcggt gtccgcctcc gcaacatcat
     1921 actgcggcac ttcctccgta ccgcgcgcaa tcaggtgggc gcaaccgacg gcaatgcctt
     1981 tgcccgccgc cacgccgtgc agcacgatac tcattattcg ccctcgccga agtagccgtt
     2041 gattaagtcg gtcagggcgc gcatcgcttc cgcctcgtcc gcgccgtcgg tttccagctc
     2101 gatgaccgta cccttggcgg cggcgagcat catcagcccc ataatgcttt tgccgttgac
     2161 gcggctgtcg tttttcgtaa cccagacttc gcttttgaat tgggacgcgg tttgggtgaa
     2221 cttgttggac gcgcgggcgt ggagtccgag tttgttgatg atttcgatgg attgtttgag
     2281 catttcggtt cccgtgttat gtatatcggc agcagacgcc gtttaaaatg ttttcctgcc
     2341 ctgccgcttc ttcagacggc atcgccgctg cgccggcaca ccaaatcttc gggcgcggac
     2401 gtgatggcga aaatgccttt tacagccgcc tctctgacac attcggtaaa ggcggcaagg
     2461 tcttccgccg ccggcgaata ttggacggcc ttaaccatca tcggcgcgtt cagcccggtc
     2521 aaaatcgccg atttgttttc gcgcacgagg cggcgggcgg cattgcaggg ggtcgcgccg
     2581 aagatgtcgg tcataatcag cacgccgtcg ttgtcgggaa attcctgaag cgcggcaatg
     2641 gcgttgttga tgatgtcgtc ttggtcttcc gtcggttgca cgccgagtat gcggacgttt
     2701 tcaggcagcc cgcccgggaa aaaatgatgt gccagcttgc ggtaggcttc gcctatggtt
     2761 tcgtgtgtga tgattaaaag ccctatcata ttatgcgtcc tgttcctcgt tgtcctgccg
     2821 gcgtatgggc gcaatgccgt ctgaaaggca ttcagacggc atcgcgtcct tattttccgt
     2881 ccaatgcgta aatctcgccc agattgcgcc agcagcccgc cgcatccatg ccgtaaccga
     2941 aaacataacg gttcggcaca tccagtccga cataatcggc tcggataggc ttgggtttgt
     3001 cgatcagctt gttggcgaac accgccgcac ggcagcttgc cgcacccatt tccaaaagtt
     3061 tggcttgaat ggcggacatc gtatgccctt cgtccaaaat atcgtccagc acgacgacgt
     3121 gcctgccccg gatttgttcc gcatcgggca tacgcttcca gttgaacgcg ccgccctcca
     3181 gcttgtcgcc gtaacgggaa acgtgaacat aatcaaaatc taagggaaaa cgcaacagcg
     3241 gcagcaactg ccccgtaaac accaccgcgc cgcccatcac gggcagcagc agcggatatt
     3301 tgccgcccaa atcgcgcgta atctcgtccg ccactttttg cagtgcggca cggcattggc
     3361 cttggtcgaa caaaagatcg gcgttttcaa gcatcgcctg tgtttcaagg cgtttggttt
     3421 ctaaatcggt catatgtcgg aatcggtcgg taaaaggaaa attataaacc aaagtatcgg
     3481 atgccgtctg aactgtcctg ctcagcggtc ggtacgcacg cgcacaaacg tggcgaattt
     3541 cggcgtgcct ttctgcgtaa agccacggta gcggtaggta atcagtgtgc cgattttggg
     3601 cgggttgtct cggtctttat ctttgaagcc gctgccaatg cggaattcgc cgtaccggtt
     3661 tttgcagccg accgcgccca accgtccggc gtttcgccct ttgccctcat agtgccgcgt
     3721 taccgtgcat tcgtcgtcgt attggctttt cagcttcaat aattggctgc tcctgccgcc
     3781 gctgtaacgg gattcgggct gacgcagcat cacgccttcg ccgccctgcg cttcgatttg
     3841 ttttaaaaag tccatcgcgt gctgccggtc gcgcactttg atttgtggga tgatggtaat
     3901 cggcgcgttc ggatgcgttt tcagccactg cgttgcgact gccaaacgtt ggtagagatt
     3961 gccctgcgcc ttgggtacat cgaaaacgtg caggcggata ccgcgccagt ctgaagaagc
     4021 agaacgcacg gtagcggaaa tctgctcgaa ctgaccgcgt ccgctataca attcgccgtc
     4081 caaaggataa ggcggaaact gcgcggtaaa gcctttgggc ggtgtaaata catatccttg
     4141 ccggctgact aaatgtttac cgtcccaata cgcgcgcacg ccgtcgagtt tctcgctcat
     4201 cgcccagcct gcaatgtcct gccctttgta ttcctgcgcc agcatcaaat ccgccgcgcc
     4261 tgctgatgca gggatgaaaa ccgccgtaaa aatcggtatg atgccgccga ttgtcttctt
     4321 aatcatctga ttcccccaat atcaaaacgg gcggcaaacc gccataaaac aaacggcaaa
     4381 cccgatgccg tctgaaaaac cgtttaggaa cacgccgatg accctacgtt acgaaatctt
     4441 ccccgttacc cccttccgcc aaaactgcac cctgatttgg gacgacgaaa gcggcgaagc
     4501 cgtcctgacc gatgtcggcg gcgacgtgcc gttcctgctg caagcgttgg caaaccgcaa
     4561 acttacgctc acggcaatct ggctgacgca cggccatctc gatcacgcgg gcggcgtggt
     4621 cgaaatgttg aaaacgcata aagtccctgt cctcgggccg catccggacg atgaattcct
     4681 gctccaatcg ctgccgcaaa ccaccgcgca atacggattt cccgtctcgc ccgcctttgc
     4741 gccgaaccgt tggctcgaag aaggcgaaac gctcacggtc ggacgctatg cctttcaagt
     4801 gctgcatatt ccgggccata cgccgggaca tatcgtcttt tattgtgccg aggcggaatt
     4861 gctgattgcg ggcgacgtgc tgttttacga aaccataggc agaaccgatt ttccgcgcgg
     4921 caaccacgcc gacttaatca ataatatccg caacaaatta ttcacccttc ccgaaaccgt
     4981 gcaagttgtc gccggacacg ggcgtatgac ttccatcgga cacgaaaagc ggcacaatcc
     5041 gtttttctaa ccgccttccc tacggtcttc agacggcatc atctgcactg atgccgtctg
     5101 aaacacaaaa ggctcagaca accgccgcct tgccggtgta cctcgccaga caaggctttg
     5161 gtaagtactt caaacaaacc caaaatcaga accagtacag ttacgccgcg ctttcggcat
     5221 tcccgccccg gctgaaacaa tatttttccg cacaagtcag actgcttcat cttctgccgc
     5281 gtattccaaa gattccgaca acgccgttgt ttcattggaa cgctcgacca aatcccgttg
     5341 aagttgtttg cctgccttca cgcccaacag acgcagtttc tcggcgcgtc cgaccagatt
     5401 cccgcgccct tcggcaagtt gcttgaatgc cgtctgaaaa ctgctttgcg cctgatcgat
     5461 gcctttgccg acgctttcga gcgtctgtac gaagccgaca aacttgtcgt acagcttgcc
     5521 gccttcgtcc gcaatcgcca gtgcgttctg attttgctgt tcgttgcgcc aaatattcgc
     5581 caccgtcctc aaagtcgcca gcagcgtact ggggccgacc agcataatcc gtttgtcgaa
     5641 acactcttgg aacaatcccg cgtcatgctg caacgccagc aggtaggccg gttcgacagg
     5701 gataaacata aagacgaaat ccaatgtgtt cacaccttcc aaatcggtgt aatccttcag
     5761 cgacaagcct ttcatgtgtg cacggatgct ggcaacgtgt gccgccagtt cgcgtgccgc
     5821 cgtatccgca tccgccgcct gcgtgtagcg cacataagct gtcagcgaga ccttggagtc
     5881 aatcacaatc tgcttgttgt cgggcaggtt gaccaaaacg tcgggctgga ggcggcgcgt
     5941 gccgccgtct tcctcttttc ggacggatgc cgcctgaacc acatattcgc gccctttctg
     6001 gaggccggaa ttttccaaaa ccgtttccag aatcatctcg ccccaattac cctgaacctt
     6061 gttctgcgtg ccggtcagcg cgttggtcag ggcctttgcc tcgctgtgca gctgcgcatt
     6121 caatccttga agccgtttca attcgttttc caacgtcagc cgctcgcgcg attctttatc
     6181 ataggtttgc ttgaccaact cgccgaaacc gtggatgcgt tcgtttagcg ggttcaaaac
     6241 ctgatggagc tgctcgcggt tctgctcggt aaaacggcgg cttttttctt ccaaaatcgt
     6301 gttggcaaga ttttgaaact gatcgctcaa gctcttgcgc gcctcgccca gtaaggacag
     6361 cttctcttca gaagcaaggc gttcctgttc gatttgcgtt gccaaacgtt cgttttcaac
     6421 cgccaaaccc tgtgcctttt cctgcaactc ggtatgcgac tgcctcaacc gctccgcttc
     6481 cgcctctttt tcctgcaaat gggcaatctg tttttcggct gcggcaaaac ggttgccgac
     6541 atcggcaagg tcgttttgca cgtcgcggac agtttggcgg ctttcttcca aatcggtttc
     6601 gattctttgg cggatttggc gttccaaggc atattggtcg gcaaacgcct tccgttcctg
     6661 tccgagctgc gttgccaaac gttcgttttc aaccgccaaa ccctgtgcct tttcctgcaa
     6721 ctcgatatac gactgcttca gccgaaccga ttccgcctct ttttcctgca aatgggcaat
     6781 ctgcttttcg gctgcggaaa aacggttgcc caaagcataa ttttcgtcct gcaaatgtcg
     6841 gtatttcccg tccaaaacgg ccaattccga tgcaatttct gcgtgtgcct gttcgacaaa
     6901 atcacatctt gccgcctttt ccgccagttg cgcgttcaaa tcggcaaact cgccctgaaa
     6961 cctgcccttc atcagcaacc atgtaaacaa cacgcccgac accaacgccg ccaaaggcag
     7021 caaaacagtc atcagttcca tcaatcatcc taatattcaa acattttcac accggacaaa
     7081 accgccgctt attccgattc tacctgtttg ttcgacatag ctccaaaaaa tatagcggat
     7141 tggctttaaa cctgttcgac atcgccttac catgctgctt gcggtttcag accttttcct
     7201 aattcaatat caatctgcca caaaccctga ttaagttccc gatgtctgac atttttagaa
     7261 tgatgccgtc tgaaatgttg cagctatgtt cagacggcat acggatttag gcttttcaaa
     7321 cggcaggcaa aatgaaaaaa gggcaaaccc taaaggattt gcccttttgt tccaaacgct
     7381 tagtgtacgt ctttccaata ttcttttttc aggaaatacg ccaaaggcag cataaccgca
     7441 aataggaaaa tcatcacgac atagcctata cgtttgcgtt gcagttgtgc aggttcgccc
     7501 atgtacacaa ggtaattgac caaatcgcgt acatatgcgt cgtactcttt ttggatcact
     7561 ttgccgttag gcagacggcg gctgtgcaaa ccggtagatt cccaatacag cttaggcttc
     7621 atctcgccgt gttcgtcttt taccataacc ggctgacctt tggcatccaa ctcaacggct
     7681 tgaacgcctt gctgttccca caacgggtgg ggcataccga ctttatcgaa tacagtattg
     7741 ttccagccgc tcggacgggt cggatcttta tagaagccgc gcatataagc gtaaaggtag
     7801 tctgcacctt tggaacgcgc aatcaatgtc aaatcgggcg gagcagcacc aaaccatttt
     7861 gccgcatctt tcgggttcat cgccgaatgc atgacatcgc cgacattatc ggtggtaaac
     7921 atcaggtttt tcttgatttc ttcgtcagtc aaaccgatgt ctttcaaacg gttgaaacgc
     7981 ataccgcttg ccgagtggca ggacaggcaa tagttggtaa agatttgtgc gccgcgctgc
     8041 aggctgactt ggtcgcgcaa atcgatatcg actttctcat agtgtgcatg accgcccgaa
     8101 gcaaatgccg cacccatggg tactgccaac agcaaggcag caaaccaatt tttcatagct
     8161 tgtttcattt tgactgccct catcagatgt tggttgcaaa caggtaagca cccacggcgg
     8221 taatgccgac gtaaacaaag aacatgattt tttgtttggc ggtgctcatg gttacgcgtt
     8281 cgggaaccgg tttgttttta tccagcttgg tatagaacgg catacccagg aagaatgcga
     8341 aatagacgaa ggacaggatg cgcgcgacca aagtacgcgt atcggttgcc accatcgcgc
     8401 ccaaaatacc caaaccgata aaggaaatga tgaacaagac caatgcggtt ttgaaaatcg
     8461 gaccgcggta acggacggat ttcacctcgc ccctatccag ccaaggcagc agggcaatca
     8521 acacgaccgc cgcgcccata ccgattacac cccaaacctg cgttcccaag aacgagggaa
     8581 tcgcacgcaa aatggcgtag aacggtgtga aataccatac cggcgcaatg tgcgggggtg
     8641 ttttcagcgc atttgccgca tcgaagttcg gcgcttcgag gaagtagccg ccgccttccg
     8701 gtgcgaagaa caatacggca cagaagacaa tcaggaatac aaccacaccc aagatgtctt
     8761 taacggtata gtaaggatgg aatgggatac cgtcgcgcgg gataccgttt tcgtctttgt
     8821 ttttcttgat ttcaacgccg tcagggttgt tagaacccac ttcgtgcagg gcgatgatgt
     8881 gcgccacaac caagccgagc aataccagag gtaccgcgat aacgtgcagg gcaaagaaac
     8941 ggttcagggt aacgtcggaa acgttgaagt cgccgcgaat ccaagtggac aaatccggac
     9001 cgataacagg gatggcggag aacaggttaa taattacctg cgccccccaa aaggacatct
     9061 gaccccaagg cagcaggtag cccataaagg cttccgccat caatgccaag aaaatcagag
     9121 aaccgaaaat ccacaccaac tcacggggtt ttttgtacga accgtaaatc aaaccacgga
     9181 acatgtgcag ataaacaaca atgaagaaga aagatgcgcc ggtggaatgc atgtagcgga
     9241 taatccaacc gccggacacg tcgcgcatga tgtactcgac tgcagtaaag gcagcaggca
     9301 gatggtaggc gttcaggttg ccgtcgggtt tgtagttcat ggtcaggaaa atgccgctga
     9361 cgatttgaat cacgaggaca agcagggcca aagagccgaa gtagtaccaa aaattaaagt
     9421 ttttcggtgc gtagtattga gccaaatgct cattccacat tttagacagg gggaaacgag
     9481 cgtccatcca gcctaacaat gcttttgctt tgctattggt ttggtttgcc ataattatcg
     9541 ttccttattc ttagtcttcg cccaccaaga tagttgtgtc gctcaagtat ttatatggcg
     9601 ggacaaccag gttggtcggg gcaggaacac ctttatatac gcggccggcc aagtcgaatt
     9661 tcgaaccgtg gcaggggcag aagaagccgc ctttccagtc tgcacccaaa tcggcggggg
     9721 caatgtcggg acggaaggtg ggcgagcagc ccaaatgggt gcagataccg atggcgacaa
     9781 ggatgttcgg cttaatcgaa cgggtctcgt ttttagcata ctccggctgc tgttccgcat
     9841 cggaattggg atcggtaagt tcgccgttca ggcctttcag gtctttaagc tgctgatctg
     9901 tacggttgag cacccaaatc ggtttgcctt gccactcggc ggtcagcagc tgacccgctt
     9961 cgattttact gacatccacc tcgacggcag caccggcggc cttggctttt tccgaaggga
    10021 aaaaactggc cacaaacggc gttgccacac ccaatgctgc cactccgccc gcgccgcagg
    10081 tcgcgagtgt caggaaacgg cggcggccgt tgttgatttc ttgattatcc attattcagt
    10141 cgtcctaata ttttgggaat accgagccat taaacgttgc aattttaccc agtttgcagt
    10201 gatactcaaa gcattattta aaataaggta aagttttatg atatttctca agactcaagc
    10261 cggattgttt tcgtcaaaat ggcacacttc caacccgaaa acctctgccg ccgattctgc
    10321 cagcgcgcgt acgccgtaac gttccgtcgc gtgatgccct gccgaaatga aagccgtacc
    10381 cgtttcattg gcaaggtggt attgggcttc ggaaatttcc cccgtcaaat acagatcgac
    10441 accttcgtct attgccgtct gaaaaaaccc ctgcgccccg ccgctgcacc atgcaacccg
    10501 tcggatttcg cgttcgggtt tgccgatgac gacaggctta cgttgcaaaa ctgtttcaat
    10561 atgcgccgcc aatgcgccga gtgtcttggc ttgtttcagg ctgcccgagt tgagcaggtt
    10621 ttgttcgccg aaccgttttt ctgtcgcaaa acccaatctg tcggcgagtt gggcattgtt
    10681 gcccagtgtg ggatgtgcat ccaggggcag atggtagcct gccatattga tgtcgtgccg
    10741 taacagtgcg gcaatccgtt cttttttcca accagtaacg gtcggcaact cgtttttcca
    10801 gaacataccg tgatgtacca aaagcaaatc tgccttctgc tccacagcaa aatcaatcgc
    10861 tgccctgctt gccgttaccg acgtaacgat tttcccgata tattccctcc cttcaacctg
    10921 caaaccgtta ggggcgtaat ctttaaacaa cgctgtctgc aatgtttcat tacaccaagt
    10981 cagaaaatcc ctgcgcaata ccatcttttt tcctaatcgc tttaaacaag cgggcattct
    11041 aatcgcaaaa tgtctggaat tcacattttt ccgatttgca cccgcatatg aattatttta
    11101 atatgcgccg gttcaatatg ccgtctgaaa cctcatggat tccatcatcg aactgcgcca
    11161 cctcaagacc ctgctggcac ttgaagaaac cggcagcgtc tcccttgccg ccaaacgggt
    11221 tttccttacc caatctgcac tttcccacca aatccgtatg cttgaaaacc actacggcgc
    11281 accgttgttc gaacgaaaat ccacgccctt gcgctttacc ccggttggag aaaggctgtt
    11341 acgcctcgct cacgacctgt tgcctcaagt taccgatgca gaacgtgatt tggcacgcat
    11401 tatagaagga gaggcaggag aattgcggat tgccgtcgaa tgccatacct gtttcgactg
    11461 gctcatgccc gcaatgggcg aattccgccc gatatggccc caaatcgaat tggatatcgt
    11521 atcgggattc caagcggacc ctatcggact gctgctgcaa caccgtgccg accttgccat
    11581 tgtttccgaa gcggaaaaac aaaacggtat ttctttccaa ccgctgtttg cctacgaaat
    11641 ggtcggcatt tgcgcaccag accatccgct tgccgccaaa actgtctggg aagccgaaga
    11701 ctttatcggg gaaaccctga ttacttatcc cgttcccgac gagatgctgg atttgcccaa
    11761 aaaaatcctg attccgaaaa acatcaaccc gccgcgccga cacagcgagc tgaccatcgc
    11821 cattatccaa ctggttgcca gcagacgtgg cattgccgcc cttccctatt ggacagtcat
    11881 gccctacctt gaaaaaggct atgtcgttca ccggcagatt actgccgacg gattgcaaag
    11941 caaactgtat gccgccatcc gtaccgaaga tacggacaag agctatctga acaatttttg
    12001 ccaaatcata cgcgaacgcg gttttgcaga tttgcccgga ctgagcgaac tggaaccggt
    12061 ctgacccctt attcaaccat acccggcagt ttttctattt tttcatgtat agtggattaa
    12121 caaaaaccag tacagcgttg cctcgccttg ccatactatt tgtactgtct gcggcttcgt
    12181 cgccttgtcc tgatttttgt taatccacta tactgttttt gatttttgcc caatctgtaa
    12241 tctttagatt gccaatggga aaccgtctac tacaaataaa aaaaccctgc gataagcagg
    12301 gttttttgaa tttccaacat taacgtttgg agaattgttt tgcacggcgt gctttgcgca
    12361 gacccggttt tttacgttcg acttcgcggg catcgcgggt aacaaaaccg gcttgagaca
    12421 aggcgggttt caacgcggca tcgaagtcga tcagggcacg ggtaatgccg tggcggattg
    12481 cgccggactg gccggtttcg ccgccgccaa caacattgac tttgatgtcg aaagattcgg
    12541 cgttttcagt cagaaccaag ggttggcgaa caaccattcg gctggtttcg cgtgcgaaga
    12601 attcgtcaac gggacggccg tttacgatga tttgacctgt acctttaatc aggaatacac
    12661 gagccactga acttttgcgg cggcctgtgc cgtagtagta tttaccgttc atgtcgcgtc
    12721 cttatttcag ttccaaaact ttgggttgtt gcgcagcatg ggcgtgttcc gcacccgcat
    12781 acactttcag ttttttaatc atggcgtaac ccaaaggacc tttgggcagc atacctttta
    12841 cagcttgttc caaagcgcgg cccgggaatt gctcttgcat ttcgcggaaa gtgcgttcgt
    12901 agataccgcc tgggaaaccg gaatggcgga agtatttttt atcttcgaat ttggcaccgg
    12961 ttacacgcag tttgtccgca ttgataacaa tgatgtaatc gccggtatcg acgtgggggg
    13021 tgtattcagg tttgtgtttg ccacgcagac ggctggcgac ttcggccgca acgcgaccca
    13081 agactttgtc ttgggcatcg atgacgaacc attcgcgctt cacctcgtgg ggtttcgctg
    13141 aaaaggtttt catagtggaa atccaaatag atatagaaag ttgtaaattt taaagacagg
    13201 attcgatttt gtcaatcgca ttaccgcgtt acggaaggat tttccggatt tcggcagact
    13261 gcatactgct ttttcgggcg gggcggcggc caatgtgaaa aaccgcatcg ttgcgatgcg
    13321 gttttgaatg ggaatccccg cgagagccgt ttcggccgaa tccgcttgaa ccttgctgac
    13381 aaggcggctg cctcgggtag tttcgggtgc gtccgcaaaa ggacgctcgc gcccactact
    13441 gctcccggca accttaagcg aacttattgg ttcaaaggaa tatatgcctt cgcggacacc
    13501 gcagggaaaa aggggttatt cctgcgccaa gcgggatagt gctttttggc aggcgttgtc
    13561 catatcggct attttacgcg caaaatcgcc gattgccaaa tcgccgccgt tcagggaggt
    13621 tttcaacagg tcgtggacga cgttgagcgc ggccataatg acgatttttt cgctgtccgc
    13681 gacgcgtccg ccttcgcgga tggcttcggc tttgccgttg agcattccga ctgcctgcaa
    13741 cagtgtgtct ttttcttctg ccggcgtgtt gacggtcagc cgggcgtgca tgacttcgat
    13801 gtggacttgt tcgatgttca tcctttaatc cttattgctg cgtttcctgc cattggggga
    13861 ggcgcgctgc cagtgcgctg attttttccc tgctctgttc gagcaggctg cggtatcgtg
    13921 tattttcttc tgtcaggctg tcgattttgt tttgcaggtc ttctttgagt ttgccgactt
    13981 ggacgagcag ggcttcgctg agttcgtcga cggcggtttc gtgttcgagt ttttgccgct
    14041 cgtgcgcccg tttgagttcg gcgacggttt ctttgaggcg gcggttttcg ctgacgaggg
    14101 tttcgaattt ttgtaccagc gtataaacgc tgctttcgag tttttcgata ttttgtttca
    14161 taaccttacc tgtccgtatg ccgtctgaag gcttcagacg gcatctgtct gttgtttatt
    14221 caaaacgcgc gctgcgttcc atcagccttt cgacgacctg ttgcggggtc atttctttgc
    14281 ggatgagttg cagcagggtt tgggtaatcg gcatgtcgat ttggtactta caggcagtat
    14341 tgaagacttc ttctatcgtg ctgacccctt cggaaacgtg tccgatttcg accagcacct
    14401 gatgcagttc cttgccttct gccaaaccca agccgacgcg gcggttgcgt gagagtgcgc
    14461 cggtgcaggt caagatgagg tcgccgatgc ctgccagccc catcatggtt ttgggctgtg
    14521 cgcccattgc ggaggcaagg cgggtgattt cagctaatcc gcgcgtaacc agtgcggcac
    14581 gggcgttaag cccgtactct agaccgtcgg acaatccggt ggcaatcgcc ataacatttt
    14641 ttaccgcgcc gccgactgcc acgccgataa catcggtact gccgtaaagc ctcatgacgg
    14701 tcgtgttgag ctgcggtacg agttcttcaa tccactcttg gttttcggag gcgaggacga
    14761 cggcgcaggg cagttgtttg gcgagttcct gtgcaaaact cggaccggaa agtacgccga
    14821 tttttttatt gtcgggcaat acttctttca atacttgaaa ggtcagcagc cctgtatcct
    14881 gctcgaatcc tttgcaggcg gcgaggacgg ggaggtgtcc cgcgccgtac tgtttgagca
    14941 gctctgcgct gcttctcaat ccggcaacgg aggttacgat aaggacaagt ccgctgtctt
    15001 tgagcgcgtc tgccaaatcc gcacacactt ccaaggtttc gggaaaggaa aagccgggca
    15061 gtccgcgttt gttttcacgc gcttcctgca tttggcggac ttggtctgcg ttgcgcgtcc
    15121 acagggatac gcggttgccg tgttgggaaa agtgcagggc gagcgccgta ccccacgaac
    15181 ctgcgccgat aacggtaatt ttcattggtc gtctttcaac atatcactgc cgttcacttt
    15241 aaaacaatcg gtgtttctct gcaagtgcgg tcagggaaat gccgtctgaa aggcgttcag
    15301 acggcatttt gccccgatgc ggcactatca gcctgtattg cgcaaacctt gcgccacgcc
    15361 gttgatggtc aggtgcacca tcagaagggc gtgcggattg tcgggttctt tacgcaggcg
    15421 tttgagcatg gcgacttgca aaccgttgag cgcgttcagg tatggaatcc tcaaagcgag
    15481 cgaacgggcg aggctgcggt tgtcgcgcaa aagctcttcg gtttgcagca ggtcgagcag
    15541 tgctttgcgg ctgcggcggt attcttcctt aatcatcccg aagatgattt ttgccttatc
    15601 gggcgattcg ctcaagccgg catagttttc cgcgagggtg atgtcggttt tcgccatcac
    15661 ttgctccata ttggagagca tggcttggaa gaacggattg ctttgggcat gttcgcgcag
    15721 ggcggtcagt gtgtcgggat tgccttcgca caaggtttcc accgcgctgc cgaaaccgta
    15781 ccaagcaggc agcatgaggc ggttctgcat ccaggaaaat acccacggaa tcgcgcgcaa
    15841 gtcttgaatc cgcgctaagg tcttgcggct ggcaggacgg ctgccgaggt tgagggtggc
    15901 gatttcctga atcgggctgg tttgcaggaa gtagtcgatg aagtcgggat gggtaatcag
    15961 ttcgcggtag tatttgaacg atacgtccga caatgcctgc atcagtttgg catcagggtc
    16021 ttttttatcc ggcaggatgc tggcttccaa agtcgcggca accaaggttt ccaagttgcg
    16081 ttgggcattg ccggggtcgg cgtatttggc ggtaatgact tcgccttgtt cggtaatgcg
    16141 gatttgtccc gccacgctgc ccgccggttg ggcgagaatg gcttggtaag acgggccgcc
    16201 gccgcgtccg acgctgccgc cgcgaccgtg gaacaggcgc atgcggacat cgtatttttt
    16261 gaagagttcg accaagccca attccgcctg atacagacac catgagctgg taacatagcc
    16321 gccgtctttg ttggagtcgg agtagccgag cataatttct tggatgttgc cgcggctttc
    16381 gagcagggca tcgtaccaat cgaggcggaa catggtttcc atgaccggac aggcgttttc
    16441 gagtgcttcg atggtttcaa acagcggcac gatattgatg cggctgtgcg gtttgccgtt
    16501 ttccaccgcc aacaggccgc tttctttcag cagcaatgcc aaggcgagca ggtcgctggg
    16561 ttgttcgcag ttggaaataa tgctttgtgt tacggcatct tcgccaaatt cgtctttgat
    16621 tttgcgcgct tcgttgaaaa ttgccagttc gtggcgggta tggtcgctgt atgtgatgaa
    16681 cgggctgtac agcggacgtt gatggctcaa ttctcgcaac aggacggctt gtttttgttc
    16741 ttcgttcagg ctgttgtagt cttccaagcc tgcgtgttgg aaaagctcgg caaccacatc
    16801 ggcgtgtttg cctgcgtgtt ggcgcaggtc gagcggcatc atgtggaagc cgaacacgga
    16861 cacggaacgg atgaggtctg ccaaacggcc ttcggcaagc aggcggctgc cgttgtcgat
    16921 aagggaacgt tgcaattttt tcaaatcatc gagaaattct tgtgccgaag catacggctc
    16981 gagaaagccg aatttgcagc ccatgcccaa accgagcgcg cgcgctttgc ccatagcgcg
    17041 cgccatgatg taagcaatgg cgcggcggta gggttcttcc gtgcgggcaa gttcttcgtc
    17101 aggcgagagg gctgccagtg ccattacgtc gtcgttgact ttgacgcggc ggatggagag
    17161 cggcagttcg cggtaaagtt tgtcgagttc gccacggtaa aaacggaaca cggcatcggc
    17221 gtggcggcgg aaggcaaaac gcaaggtttc ggcagaaacg aacggattgc cgtcgcggtc
    17281 gccaccgatc cagccgccga ttttgaggat attcggaacg cagacatcgg gatagaccat
    17341 ctgaaagtcg tgttccatat tgcggtagag cttgggcagg gcttcgaaaa agctcatcgg
    17401 gaagatggac acgccgttgt tgatttcgtc gttgacgctg agtttgtggc ggcgcgtttc
    17461 gctggtctgc cacaagccca gcaggatagt gtcgatttcg cggcgcagcc gtgccagcgc
    17521 gtcggcattg gtgcagcgtt cgcgttgcgg caacagtgcg cggatgcggc ggttgaagct
    17581 taagacggtt tggcgttgca cttcggtcgg gtgcgcggtc aaaacggcgg taacggacgt
    17641 attgtccaac tgccgctgca ccgatttgcc gtcggctttc cccgctttga gcctgcggac
    17701 ggtttccgtc aggctgcctt ccgcgccgcc gcgtccggct tcttcgtgga tttggcggcg
    17761 gcgttcgtgg tgcacgtctt cggcgatatt caaaatctgg gcgaacaggc cgcaggccaa
    17821 ggttaaatcg tgggtttgtt gttcgtccaa ttgcggcaat actttttcaa tcaatgccgc
    17881 gctgtcgtcg gaagtggaca agagtttgac cgtttcgaca accaatggcg aggcttcttc
    17941 gtgcaggagg ttgaacaggg attgtttcag aaattccgcg tccgccgcca aagccgcgtc
    18001 cttcggattg ttcagaatat gcagttgcat gatttttctc tcattgccgt aaatactgta
    18061 aatgtacctc aaatgccgca tccgtgccaa accgttcaca ctttaaccgc ccgtgtcccg
    18121 aaatgccgtc tgaagttgaa cgccgcccga cggcagcgtt acaatcgccc acaactgttt
    18181 tttccgaaca tcatcatgac cacgaccgaa cacgacaacg acgatgcatt cctgctgcgg
    18241 tacagccgcc acatcctctt ggacgaaatt ggcatcgaag ggcagcagaa actttccgcc
    18301 gcgcatattt tggtcgtcgg ctgcggcggt ttgggtgccg ccgccctgcc ctatctcgcc
    18361 gcttccggca tcggcacgct gaccatagcc gattccgaca cggtcgagct gcacaacctg
    18421 caacgccaag tcgcatttga cgagggcgat gtcggcaagc ccaaagccga agccttggca
    18481 gaccgcctga aacgcatcaa ccataacgtc gatgtccgca ccgtcaacga aaaactcgac
    18541 ggctgccgcc tgaccggttt ggttcaaacc gccgacatcg ttttagactg ctgcgacaac
    18601 tacgccacgc gccaagccgt caaccgtgtc tgcgtacaaa cgaaaacacc gctggtttca
    18661 ggagcagcgg tgcgctttga agggcaactt gccgtgtacc gtcccgactt gcccgactcg
    18721 ccgtgttacg cctgcctgtt tgacggcgga tcggcttcag acggcatctg ttccctcttc
    18781 ggcgtattct cgccgctggt cggcatcatc ggctgcacgc aggcggcgga ggctctgaaa
    18841 atcctgctag acgtgggcga accgtcgcac ggcaggctgg cggtttaccg tgccttggaa
    18901 gggggctggc aatatttcga cctgccgcgc aaccccgaat gcccgatttg cggcacggaa
    18961 cggtaaaacc ctgccgccgt ttcagacggc ataaagacgg atgcggagga acggttttaa
    19021 aaatcggcgg tgttttgaca tagtgtcgat aagcatactt atcttcaata tactcaacag
    19081 gataattgtt tgcctaagca aaaaataatc gccttttccc tgacgggcgg gcgaaatcca
    19141 cggattgcaa aaacacaacc tgaaaattta catttctttg caaaaaaaaa aaaaaaaaaa
    19201 aaa
//
LOCUS       DAWEWP010000027        18845 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWEWP010000027 DAWEWP010000000
VERSION     DAWEWP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18845
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.027"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            52..1005
                     /locus_tag="WGN53_000670"
     CDS             52..1005
                     /locus_tag="WGN53_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ0139685.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVSKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1109..1432)
                     /locus_tag="WGN53_000671"
     CDS             complement(1109..1432)
                     /locus_tag="WGN53_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139686.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1605..2966)
                     /locus_tag="WGN53_000672"
     CDS             complement(1605..2966)
                     /locus_tag="WGN53_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ0139687.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASQEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVEGT
                     DWE"
     gene            complement(3327..5027)
                     /gene="recJ"
                     /locus_tag="WGN53_000673"
     CDS             complement(3327..5027)
                     /gene="recJ"
                     /locus_tag="WGN53_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ0139688.1"
                     /translation="MSVKIQTRSINTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLAALLPYQTLTNCEAAAGRLADAVGRKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLTALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTTFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
     gene            complement(5079..5813)
                     /locus_tag="WGN53_000674"
     CDS             complement(5079..5813)
                     /locus_tag="WGN53_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139689.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTLMLWGMNNPVSETITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTDGLCLRYDTDRPDDIAKLKQLEFEAVELDKQTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFANILYTPPLFDTGCG
                     GRGTGFACGGSGCGRGCRPQMNSNAV"
     gene            complement(5860..6366)
                     /locus_tag="WGN53_000675"
     CDS             complement(5860..6366)
                     /locus_tag="WGN53_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ0139690.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            6521..7852
                     /gene="pmbA"
                     /locus_tag="WGN53_000676"
     CDS             6521..7852
                     /gene="pmbA"
                     /locus_tag="WGN53_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ0139691.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGFAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8149..8352)
                     /locus_tag="WGN53_000677"
     CDS             complement(8149..8352)
                     /locus_tag="WGN53_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0139692.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8595..8897
                     /gene="clpS"
                     /locus_tag="WGN53_000678"
     CDS             8595..8897
                     /gene="clpS"
                     /locus_tag="WGN53_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ0139693.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8899..11196
                     /gene="clpA"
                     /locus_tag="WGN53_000679"
     CDS             8899..11196
                     /gene="clpA"
                     /locus_tag="WGN53_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ0139694.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDEDDGNYSSDG
                     MDDDNENRAKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPL
                     LVGEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKS
                     VLKQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEY
                     RTIFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELS
                     ARYINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVSRIPEKTVSH
                     DDKQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGK
                     TEAAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHC
                     VLLLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSL
                     GFTAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLD
                     KKVEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRI
                     DWDAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(11260..12264)
                     /gene="rfaD"
                     /locus_tag="WGN53_000680"
     CDS             complement(11260..12264)
                     /gene="rfaD"
                     /locus_tag="WGN53_000680"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ0139695.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LNWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVTFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(12305..13423)
                     /locus_tag="WGN53_000681"
     CDS             complement(12305..13423)
                     /locus_tag="WGN53_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0139696.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEVLLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(13425..14660)
                     /gene="dcm"
                     /locus_tag="WGN53_000682"
     CDS             complement(13425..14660)
                     /gene="dcm"
                     /locus_tag="WGN53_000682"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0139697.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFSRVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(14697..15668)
                     /gene="hldA"
                     /locus_tag="WGN53_000683"
     CDS             complement(14697..15668)
                     /gene="hldA"
                     /locus_tag="WGN53_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ0139698.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(15723..16463)
                     /gene="pyrF"
                     /locus_tag="WGN53_000684"
     CDS             complement(15723..16463)
                     /gene="pyrF"
                     /locus_tag="WGN53_000684"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ0139699.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(16989..17636)
                     /gene="adk"
                     /locus_tag="WGN53_000685"
     CDS             complement(16989..17636)
                     /gene="adk"
                     /locus_tag="WGN53_000685"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ0139700.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            17851..18690
                     /gene="htpX"
                     /locus_tag="WGN53_000686"
     CDS             17851..18690
                     /gene="htpX"
                     /locus_tag="WGN53_000686"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ0139701.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
BASE COUNT         4602 a         5114 c         4522 g         4607 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatcttgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggc tttctgcccg gagacctgac ccaaaaagtc
      601 gacccctacc tccgtccgct ttatgacgcg ctttacgacc tgatgggctt cgaccgcgtc
      661 agcaaactga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 aacgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctctg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa tcgggcagag aaaaacggat acggaaacca agctccgtat
     1081 ccgtttcttt atccgtccaa ccgatacatc aatcgaattt tctgcatcgg attgcctcat
     1141 cttcgcaaaa cattgccgaa gtttcgcggt tgcacgcctg acgcactttc tgccgcgcca
     1201 cgccttcatt ccgatcgctt gcctcaaacg tcttctgaaa aggcgtaacg ctgcatacat
     1261 acatggtgga atttgaaccg aaaaggtcga ttttgacggt attgctgccg ctttccaata
     1321 cggcgacacg gtgttccaac tgcgccagac gtgcttccaa atcgccgatt ctgtcggcaa
     1381 atgcaggcat ggcggcacac gcggtcaaaa tgcccaaaat acattttttc atcaattttt
     1441 ccttgtttct tgaaccctac ccacccggac atcctttttc cgagaggcag ggtcagactt
     1501 tatttacggg gatgaatccc cttacaaatc aggataatac atagaacggc actttatgct
     1561 tcatcccatg tgttgaaact tcattgctcc ttgtctgttg acccttattc ccaatccgtt
     1621 ccctcaacct tcggcttggg tttgcgcctg cgacggcggc gttttttcgc ctgtccttcg
     1681 tttttttcgt gccgggcagc agcctcgttt ttggtcattt ccgtccgttg ttcctgcgat
     1741 gccgtctgaa acgccgtcca ccactcggca agggcgcggt ccgcattgcc ggtttcggcg
     1801 cgcaagagca ggaaatcata ggcggcacgg aaacgcgcct gtgcaaacag tttatgcgga
     1861 cgtgcgcctt tgcggttctc aaactgcggc tgaaacatcc aaatttcgcg catcgtggcg
     1921 gaaaagcgtt gcggcacgcc ccaaccgcgt tcgacggttt cacgcatcgt attgattgca
     1981 tcggacaggg cgggcgcagg tttcaaaccc tgttgcagat tgcttttcca atggcgttcc
     2041 aactcgggcc acatcagagc cgccagtaca aaaccgaccg aaaccgattt gtcagcacgc
     2101 agccgctcat cggtattttt cagggcaagc accgtcattt ttccggcgat gccgtctgaa
     2161 acgcgcaagg cattgagcag cggatggatg tcgtccggta tgtcaaatcc gttcaaacgt
     2221 ttcagacact cgcgcgcgtg ccctgaaaac agcagtttca tgatttcgtc aaacagcctc
     2281 gctaccggtt cgtgcttcag acggcatatc gattcggcaa tcggtgcggt ggtttcttcc
     2341 gacagctcaa agcccaattt gcccgacagg cggatggcgc gcaaaatcct gacagggtct
     2401 tcctgatagc gttcggcggc atcgccaatc ataaccagcc tgcgggcggc aacatcggca
     2461 atcccgttgt ggaaatccaa aatctcttct ttttcaggat cgtaatacaa ggcattgcag
     2521 gtaaaatcgc gccgcatcgc atcttcttcg atgctgccgt aggtgttgtc tttcatgatt
     2581 ctgccgtgcg cgttctgatg aaccttcgca ccgccgcgaa acgtcgttac ttcgataatc
     2641 tccgcaccgt tcatcacatg gacgatttga aaacgcctgc cgatgatgcg gctgcggcgg
     2701 aagagtttgt gcacctgttc gggcgtggca tcggttgcga catcgaaatc tttgggttcg
     2761 atgccgagca gcagatccct gaccgcgccg ccgaccacat aagcctgaaa ccccgcccct
     2821 ttcagacggc gtatgacgtt ttcggcggca aagctcaaca tttcggcacg gatgttgtgt
     2881 ctttcggcag gaatgaccgt tttactttcc gcttttttac tgctccgacc ggaaggcagc
     2941 atcttattca accatttttt cagcataaat attctaaccc gaagagcggc atctccacgg
     3001 gcaaccgtgt tcagacggca tcaggcggta cgattccgca agatgccgta accataaaca
     3061 aaaggcgtag aaactacgcc gtccgaatca cgccgccctc gcggcggcgg caacgcgcca
     3121 ttataacaga ttgcccttta aaaaaatcag accctgcttt tgtggcggag tctgaaattt
     3181 gatgcacctt atatattgcg tccctaagaa gggatgatta acaaaaatta acgcccttta
     3241 ctttcttttc cgattgggaa cattcccgtc gtacaaacag gcaaatgaag gaacagaaat
     3301 cacattcaaa cggcgttccg ccgcctctac accgcttccc agtaatcgat atacagctgc
     3361 aattcgagat tgttgcgcca ttcgttggca acggggcggt aaaccgtgcg gatgtattcg
     3421 gggatgtctt cgctgcaacg ccaaaacatc gcttcaaatt cgcagccgtc tttttgcagc
     3481 cagacttttt tgtgtttgcc ctccgcgccc aaaggttgct ggcggacgac gtggaactcg
     3541 tcggtaaagc tcggcggcgc gaagccctgc ccccaaacgt gacgggcaag gttttgcgcc
     3601 tgttccaacg tgatgtcgca ggcgggcagg ctgccgtcgg tgatgaaggt ttgcgacaaa
     3661 tcgtcttcgc acaccatttc gcgcacggct tcttcaaagg tcgtctgaaa cgcgggaatg
     3721 ttgtgttcaa gtatgctcaa acccgccgcc atcgcgtgtc cgccgaattt taaaatcaaa
     3781 tcgggatggc gtttggacac caagtccaaa gcatcgcgca ggtgcaggtt gggaatggaa
     3841 cgccccgaac cgcgcacttc gccgttgtcg gcaggcgcaa acacgatggt cggacgataa
     3901 aaacggtctt tgaggcggct ggcgacgata ccgaccacgc cttgatggaa gtcgtcgcga
     3961 tacgccacca aagtcgtctg acctgaaggc agggtttcag gaaaggcgtt cagcgcgtct
     4021 tgcagcatag actgctcgat ttcgcgacgc tcgatattga ggttgtttaa ctgagccgcc
     4081 agttcctgag cttcggaatc atctcgcgcc aacaggcagg cgatgccgac cgacatatcg
     4141 tccagccgtc cggcggcatt gatgcgcggg cccaacgcaa aacccatatc aaacggctgc
     4201 gccttgcgcc agtcgcgccg cgccacttca aacaaggcgc ggatgccggg gcgcattttg
     4261 cccgaacgca tccgtttcaa accttgcgac acgaggatgc ggttgttgtg gtcgagaggg
     4321 acgacatcgg caaccgtgcc gagtgcgacc aaatccaaaa gatcgcccag attcggctct
     4381 ttcaggccgt ctgaaaaata attgcggcgg cgcagttcgg cacgcaacgc cgtcaacaca
     4441 taaaaaatca cgcccacgcc cgccaagctt ttgctttgga aaccgcagcc tttttggttc
     4501 ggattgacga tgatgcagtc gggcacggta tcggcaggca gatggtggtc ggttacaatc
     4561 acatccaaac ccagagcctg cgccctcgcc acgcctgcga tgctggcgat accgttatcc
     4621 accgtaatca gcaaatccac gccctgcgcg gcagcgattt cggcaagttc gggcgttaag
     4681 ccgtagccgt gttcaaagcg gttgggcaca aggaaatcca cttttgcccc catcgccgcc
     4741 aaaccgtcca aaccgacggc acacgccgtc gcgccgtcgg catcgtagtc ggcgacaatc
     4801 aggatttttt ccttgcgccc aaccgcatcc gccaaacgtc cggcggcggc ttcgcagttt
     4861 gtcagcgttt gatacggcaa aagcgcagca agcttgtcgt ccaattcggc aggactttgc
     4921 acaccgcgcg aagcacaaag ccgggcgatt aaaggatcgg caccggcggt gagcaaatga
     4981 ttaaaaacgt cggtattgat ggatcgggtt tggattttga ctgacataac gtggggtttt
     5041 actgtgcgtg tgtgcttaaa atgccgtctg aaagcttttc agacggcatt gctgttcatt
     5101 tgcgggcggc atccacgacc gcacccagag ccgccgcagg caaagccagt accgcgcccg
     5161 ccgcatccag tatcaaaaag ggggggcgta tataagatat ttgcaaacag cttggatttg
     5221 tcggtatgtt tttcagtaac cgtgtaataa atatcggcag gcacactttg ctcaaaatga
     5281 taatcggcgt tcagtttttg cggcgtggcg tagtatttgc ctttggcaga gacgcagcgc
     5341 gtgtaaatag tctgtttgtc gagttcgacc gcttcaaact caagctgttt cagcttggcg
     5401 atgtcgtcag gcctgtcggt atcgtagcgc aggcaaaggc cgtcggtact gaaattctgg
     5461 ctgccgggcg attcgagttt gaccggcagg gcttggtggc gggcatagct cggggtatcc
     5521 tcaactattt ggaagggctt gtccagcccg gccttcaaaa tgcccgtcag cttcgccgaa
     5581 tcttcaggat tgacgacgaa ccagtatttt ccgcccatca tcaccaggct gcccttttcc
     5641 aattgggcat tgtcttcggc aaccacaccg aaggcgcgga tttggtcttt gtcaacgtgt
     5701 ttgcgggtga ttgtttcgct gaccgggttg ttcattcccc acaacatcaa cgtacagccg
     5761 ttcagcatca aggttgccgc caaaacggcg gcggtcgttt tacggaacaa cattttatcc
     5821 tccaatctaa ataacggcgg atgtcgtctg aaaatatgcc taaatttccg cgtccccgtt
     5881 ttccatcacg gttttcaact cttgaaacag ggcgcggaaa ttttttggtg gtttgttttg
     5941 ctcctgctct tttttggtat tgcggatgag tgtcctcagc ttgcccgcgt ccgcatgcgg
     6001 aaaatccgac ataaactgcg tcaacgcgcc gtcgtctgcc agcagctcca tccgcgtccg
     6061 ctccacacgt tgcaaaaagg cgttgtgcgc cgcatcgtcg ccgcgcagct tggcaagaaa
     6121 tgcctcgatg ggcgcgggat cggtatcgcg catcagtctg ccgataaatt gcgcctgccg
     6181 cttgagcgcg ccgttggatg tgattttttt ataggtaacg acggcttcgt acaaatcttc
     6241 gtccaagccg atttttttca acgtgtcgct tgagagcttg gtcagctcca tacccaaatc
     6301 ctgcaaaccg ttcatctgct ttttcatttg ggttttgctg acccattcgt cttcttgttc
     6361 aaacatcttc attctcaaat tttatccgac gggcgcattt taacagaatg attccgcgtt
     6421 cagacggcat tttgcaccgt ccgccctgca aacggcacgc ttttgcccct gccgcccgaa
     6481 acggttaaaa tgccgcaacg ctccaccaag aaagatttct atgctgttca accacaccgc
     6541 ttccgaactt ctcgacctct gccgccgcac gctcgacttg gcaaaagcag cgggcgcgac
     6601 cgccgccgaa gccgatttca gcgaatccat cggacaaagc gtcagcgtgc ggctgggcga
     6661 aatcgaacaa atcgagttcc agcaggacaa gtcgctggac attaccgttt acgtcggcaa
     6721 acgcaaaggc cgcgctggca ccgccgattt ctccgaacaa gccctgcgcg acaccgtccg
     6781 cgccgccgcc gacatcgccc gccacaccgc cgaagacggt tgcgccggac ttgccgaccc
     6841 cgagttgatg gcgcaacaca tcggcgaccc cgacctttac cacgaatggg atttggatac
     6901 ggaagccgcc gtcggctttg caaaacaatg cgaacaagcc gccctgaacg aggatgagcg
     6961 catcgaaaac tccgaaggcg cggcggtgca aaccggccat taccaatacg tttacggcaa
     7021 cacccacggt tttgccgcac accggcaagg cacgcaccac agcatttcct gcagcgtcgt
     7081 tgccgccgac gaaaacggga tgcagcgcga ctactggtac gattccgcct gccgccatcc
     7141 ggatatggac agccccgaaa ccatcggtca aaccgccgcc cgccgcactt tgcgccgact
     7201 cggcagccgc agcatcccga caggcagcta ccccgtcctc ttcgatacca ccgtttcggg
     7261 cggccttatc ggacacctcg tcggcgcact ctccggcggc gcgctctacc gccaaagcag
     7321 tttcctgatc gacagcatcg gcaaaaaagt cctgcccgat ttcctcaacc tgcgcgaaga
     7381 accgcacatc ccccgctctt tccgcagcag ctattttgat gcggaaggcg ttgccaccgc
     7441 accgcgcttc gtgattcaaa acggcattgt cgaaggctat ttcctcagca gttacagcgc
     7501 gcgcaaactc ggtatgcaga ccacgggcaa cgccggcggc gcgcacaacc tgtatttgaa
     7561 ccatacgcac gaaacgcaat ccgacctgct gaaagaaatg gggacgggat tattggttac
     7621 cgaactgatg ggacagggcg cgaacaccat taccggtgac tactcgcgcg gcgcggcggg
     7681 tttttgggtg gaaaacggcg tgattgccta ccccgtccac gagattaccg tagccggacg
     7741 cttgcaggat atgtaccgcg acattgtcgg cgtggcggat gacgctttgc ggcgttcgtc
     7801 caacaaaatc ggctcgattc tgattgcggg gatgacggtt gccggaagct gaacgccctg
     7861 ccgatgccgt ctgaagacga aggcagcacg ttttgccccg tgcctgcccg tttgccgttt
     7921 tatgccgcac cgccttttgc cgacaacatt gcacattttt tcatcagcac tgattttccc
     7981 ttatccgtca aaatactaag ccacttgtcc acaacagggt acacacaagt tcaggataag
     8041 gcgtgcgggg attggggttt ttccgccgcc ggcaaaacgg cagacaaaaa cggcgggaca
     8101 ttgcctgtcc cgccgttttc aaatatacat ttgaccgtac atcaggaatt aagcagcctg
     8161 aatgttggcg gcctgtttgc ctttagggcc ggtggttacg tcgaaagaga cgcgttggcc
     8221 ttctttcagg gttttgaaac cttccatatt gattgctgag aagtgagcga acaaatcttc
     8281 gccgccttca tcaggcgtga tgaaaccaaa acctttagcg tcgttaaacc attttacgat
     8341 accggttgcc attagaaact tcctatactc aaaaattaac aaaatcagca aaacaaggca
     8401 tacggcaaac cgagccgtct ggcgttcctt ccagcttgat atactcggcg gatgcccaat
     8461 cctgcttaac cgtcttttta catttgttaa gacaaaacgt caagctatgt tttcaaaaaa
     8521 gtgggaaaat aggcaaaagt accgaaaata caacaaaaat gccgtctgaa cgcggctttc
     8581 aaatgggaca cactatgacc gcgcaacacc aatccgacac gctcttgcac cgtctgaaca
     8641 ccctgccgcc gaaacgttac ggcgttttcc tattgaacga cgattacacc acgatggaat
     8701 ttgtcgtcga aatcctgacc gaaatcttta tgctcggaca agaacaggcg gtagcggtaa
     8761 tgctcttggt tcatcacgaa ggcaaaggcc tgtgcggcac ttacacgcgc gatattgccc
     8821 aaaccaaaca acaacaagtc atgcagcggg caaaagccga agggcatccg ctgcaatgta
     8881 ttgtcgagga gatttaatat gcttgcaccc gaattggaac agattttgca gcagctttac
     8941 cgcgaggcgc gtaaggctca ttatgaattt atcagcctcg agcatctgct tttggtactc
     9001 atcgaagaag atgcctccgt gccgaacgtc ttaaaactat gcggcgcgga tttgaaagtg
     9061 gtgtccgaac agctcgccgc cagcgttgcc gaaaacaccc ccctgattcc cgaacacctt
     9121 ttagacacgg tcgaaacacg gcccacgctc ggcttccaac gcgtgatgca acgggcgatg
     9181 gtgcataccc agtctgccgg aaaagccgca gtcgaaccgt tggacgtttt ggtcgcgctg
     9241 atgagcgaaa ccgacagcca caccgtctat ttcctcaagc tgcaatcggt tacgcgtttt
     9301 gaagttttgc gctgtattgc ccacggctct cccgatgaag atgaagatga agacgatggc
     9361 aactattctt cagacggcat ggacgacgat aatgaaaacc gcgccaaacc gggcaaaaac
     9421 cctttatcgg cgtacaccgt caacctcaac gccgaagtca aagccggccg tatcgaccct
     9481 ttgattggtc gcaaacacga aatggaacgg ctggtgcaaa tcctatgccg ccgccgcaaa
     9541 aacaatccgc ttttggtcgg cgaagcgggc gtgggcaaaa ccgcgctggc ggaaggtttg
     9601 gcacatcaaa tcgtcaacgg cggcattcca gacgcgctca aagatgccga agtgtacgcg
     9661 ctggatatgg gctcgctgtt ggcgggcacg aaataccgcg gcgactttga agcgcgggtc
     9721 aaatccgtct tgaaacagct cgaaaaaatc ccgcacgcca ttttgtttat cgacgaaatc
     9781 cacaccatca tcggcgcggg cagcaccagc ggcggcacca tggacgcgtc caacctgctc
     9841 aaacccgcgc tggcaaaagg ttcgctgcgc tgcatcggcg cgaccaccta cgacgaatac
     9901 cgcaccattt tcgacaaaga ccatgcctta agccgccgct tccaaaaaat cgacgtggtc
     9961 gaacccaccg tttccgaaac cgttcaaatc ctgcgcggct tgaaaccgat gtttgaagcc
    10021 ttccaccaag tccgctacac tcaaggcgca ctcgaagccg ccgccgaact ctccgcacgc
    10081 tacatcaacg agcgtttcct gcccgacaaa gccatcgacg taatggatga agcaggcgcg
    10141 gcgcaacgga ttctgcccaa atccaaacag aaaaaagtca tcggcaaagc acaaatcgaa
    10201 accgtcatcg ccaaagtttc gcggattccc gaaaaaaccg tgtcgcacga cgacaaacaa
    10261 gtgctgcaat tcctcggtcg cgatttgaaa aacatggttt acggtcaaga aaacgccatc
    10321 gacgcgctgg ttgccgccgt caaaatgtcg cgttccggcc ttgccctgcc cgacaaaccg
    10381 ataggcagct tcctcttctc cggcccgacc ggcgtgggca aaaccgaagc cgccaaacag
    10441 cttgcctact cgctgggcgt accgctgcaa cgctttgata tgtccgaata catggaacgc
    10501 catgccgtat cgcgcctcat cggcgcgcca ccgggctacg tcggctttga acaaggcggc
    10561 cttttgaccg aagccgtcaa caaacagccg cattgcgtat tgctcttgga cgaaatcgaa
    10621 aaagcccacc ccgacatttt caacgtcctc ctgcaagtca tggacgcagg caaactgacc
    10681 gacaacaacg gcaagagtgc cgatttccgc aacgtcatcc tgattatgac caccaacgca
    10741 ggcgcggaaa gcctcagccg ccccagcctc ggctttaccg ccaagcgcga acgcggcgac
    10801 gaaatgcagg caatcaacaa gctcttcacg cccgagttcc gcaaccgctt ggacgcgatt
    10861 atcccgtttg cacccttgtc cgaacccatc atcgtcaaag tcgtggacaa attcctgctc
    10921 cagctcgaac accagctcct cgacaaaaaa gtcgaagccg aattcacgtc ggcattgcac
    10981 aaatatctgg cggaaaaagg tttcgacccg caaatgggcg cgcgcccgat gcaccgcctg
    11041 attcaggaaa aaatccgcaa accgctcgcc gacgaactcc tgttcggcaa actatccgac
    11101 ggcggcttcg tgcggataga ctgggatgcg gcaaaagaag aagccgtgtt gaagtttaag
    11161 aaaagcaagg tcaaaataaa aaccgcctcc gcgtaaaatc aaaaatgccg tctgaaattt
    11221 cagacggcat tttctttcaa acaaaattac caatcgtgtt taagccaaat tttccagcat
    11281 ccatttgacg tagcggtcga cgcctgattt gacatcgaaa aattcttcct tatatccggc
    11341 ttcgcgcaat ttggtgatgt cggcttgggt gaagctctgg tatttgcctt tgagtgcgtc
    11401 ggggaagggg atgtagcgga taagttcttc ttccaccagc tctttcaagc tcatttcagg
    11461 cttgccttcg gcggcgcggc aggcgttgac ggtggcggcg gcgagttcgt taaactgttg
    11521 gctgcggccg gtaccgaggt tgtagatgcc ggaaagttcg ggatggtcga agaagtagag
    11581 gttgactttg gcaacgtctt cgacgctgac aaagtcgcgg gtttgttcgc cgttgccgta
    11641 accgtcattg ctgccgaaca ggttgacgta accgtgttcg cggtattggt ggaaatggtg
    11701 gaaggtgacg gatgccatgc ggcctttgtg ttgttcgtgt tgtccgtaaa cgttgaagta
    11761 gcggaagccg acgacttggg cggtgagacc ttctttcatg cggcggcgca atacttggtc
    11821 gaacaggaat ttggagtagc cgtacacatt aagcggtttt tcgagttcgc gctcttcgcg
    11881 gaagatttcg cctttgccgt aaaccgccgc actggaggca taaaggaagg gaatgcgttc
    11941 gtcctgacac cagttcaaca aatccagcgt gtactggtag ttgttttcca tcatatacaa
    12001 accgtcgtgg ttcatcgtat cggaacacgc gccttgatgg aaaacggctt cgatgttttg
    12061 ataaggtaaa atgtgttccc tcacttggcg gatgaattcg tgtttgtcga gataatgggc
    12121 gatttcgcac tcggcaaggt ttttgaattt ttcgcctttg ctcaaattat cgacggcgac
    12181 gatgtcggta ataccgcgtt gattaagtgc tttgacgatg ttgctgccga taaagccggc
    12241 cgcgcctgtt acgatgatgg tcatattcgg tttcctttgt tttgagtaaa attaataaac
    12301 ttagttacaa gaaatcatcg atattgtcct gaaacacaaa tttgcgatga tattccaaat
    12361 atttttctct tccttcggga tatattctca tcttattttt tgcatttgga ttgatattaa
    12421 gtttttccca tgtgtaacgg cttagtcgag taccgcaaat caaacgacca tcatccaaga
    12481 aagtaatata accttgatcg aacaaccaat cataagtcgg cgttaaagct aacccattta
    12541 aataatctaa tgcctctttc tcatttttct cagtaataca aaccatataa ggtttaatat
    12601 gactagccct taacaaaacc tcatctgtaa ttaatgtaaa tggacattgc ggcatatgat
    12661 ttataacatc cttgcgatat ttctgctgcc cttttctact tttctgttta atgatttctt
    12721 gctctacgaa ttgattaaat tctgaagccg gtatttctaa tttttctctt gataagagtt
    12781 gcgggtgcac gatagagcga aattgataat ccaaaaatat tcggaaataa aataatggtt
    12841 ctgaatcttc tatatctttg acaggcaata gtttaagaat tgataaataa ctaattttgg
    12901 gcaatacaat cttacgccaa attgaccata tatcatcctc cgaacggata taacccctac
    12961 ggtaaccttt agaattttcc aaatggtctg atacatcatg gattgaaaag aaaatatttt
    13021 ctgtaggcag attattaata cttaaaacgt aatcatcata aaaccttgaa atatcatgat
    13081 gatatttctg ttcttgtttg tgatattcaa cttttgcatc aaccaaataa cgctgtaaat
    13141 ttttcttatt aaaaaaacaa tttttctgaa tgagaggata ttctaccctc cttccatttt
    13201 ttgtctcagt aatcccccaa ttttcatact caaaaaatgt acttagtttt cgaccactat
    13261 catcaatata actacctaca tatttgcgtg cttccccatt accaccaaaa acagataact
    13321 tgttattcct gtgaataaaa ctatcttccg cacgtaaatt ttgaatacta tcgacaataa
    13381 aatactctat gttttcaata gtaattcttt gaaaatccgg cattttactc tccataaagc
    13441 gtaatatcca tttgttttaa tatagcgatt aatacatcaa caacaatact gtttcccgct
    13501 tggcgataca tttgagtatc actaactaaa attttaaaat catctctaaa acccattagc
    13561 cgcaaacatt ccctaggtgt taatttacga atacgtccac gattatgagt aacataatta
    13621 tcaaccccgg cacgatgcat tttatgcata gtttgcaata atgggcgagc tacaggcaaa
    13681 tcagtttccg tactggtttt aaaatttttt gttcctcctg ccaaaacata atttttgatt
    13741 ttttcagaaa gataatattt ttcctcaacg ttatttacat caaaaataaa gtcatcaaat
    13801 tcagattcgc gggctgcctg aaaaataaaa tcaccatgcc aattaaattg ttgatttgct
    13861 ttttggcata aggcaatttc tccattaatc tgtgtataac gtttttgtct atttttagaa
    13921 ctggtaacaa atttcacacc cttttcacgt aaaaaatatt tgctatcagt atagtcctcc
    13981 aaaaaatctt gcatagtatg ttctaattca atcttttcag gaaactgaaa accatttata
    14041 ggaggggtat gaaagccaac aacaaaaata cgctcacgat gttgaggaat cccataatcc
    14101 ttactattca ttatttggaa atataagtca taaccaagtg aataaaaaac actttttaca
    14161 actttccaag tttttccatt atcatgattg agcaagccct ttacattttc ataaataaat
    14221 atctttggtt ggacttcatc cacaacgcga gaaaattcat agaataatgt tcctcgtgta
    14281 tcttctaatc ctgcacgttt gccaaccatg gaaaatgctt ggcaaggact gcctccaact
    14341 aaaatatcaa cttgatttct aaactttctc gcatcaaatt gagtaatgtc gttatgccaa
    14401 aaatcttcat ttaatttata gtttccaaga taactttttt taacgtatgg atcaatatct
    14461 cctgcaaaaa caatggtatg gtttaaattt aatcggtgga atgcctgttc aaccgcacca
    14521 atcccgctga atacggttgc taatctaata tgtgaatcag gtttaagaaa agttttagat
    14581 ttccaacctt gttgactggg aaagagcaaa gttttttgta atcgagtatc gtgtgtctgt
    14641 gccattgtcg aaatagtcat acttatatcg ttctgtttat cttatcaata tgaaaactac
    14701 attgttgatt gccctgacaa tgccttgatc aattcggcaa acgagcaaac cgccgtaccg
    14761 agtttcgcca cgacaacccc ggccgcagta ttggcaaggt acatggcttc gggcatggtg
    14821 cagcctgccg ccaaacccaa gcccattccg gcaatgacgg tgtcacccgc accggatacg
    14881 tcgtaaactt cttgggcgcg ggtgggctgg taaatcggtt cgccttcgct gaacaaggtc
    14941 atgccttctt cgcttcgggt cagtaaaacg gcggtcaggt cgaggtggcg gcgcaggttt
    15001 tgcgcttttt cggtcagctc gctttcgttt ttccaactgc cgaccacttc tttcaattcg
    15061 gcgcggttag gcgtaatcag agttgcaccg acatattttt cgtaatcgtc gcctttgggg
    15121 tcgattaaga cggttttgcc ggcgtgtttc gcccaatcga tcatatcgga gatatgcgac
    15181 aggccgcctt tgccgtagtc tgaaaaaatg attgcgtcgt attcgggcaa gatttcgcgg
    15241 tatttctgct tgatttgttc caacacttcg cagttgggat gttcttcaaa atcaagacgg
    15301 ataagctgct ggttgcgggc gacgacgcgc agtttgacgg tggtggcgat ttgtttgtcg
    15361 cgcatcagat aggaggcgac gccgtcctgc accatcagcg catcgagcgc gtcggcggct
    15421 tcgtcgttgc cggttacgga caacagccct gccctgccgc ccaacgaagc gatgttgcgc
    15481 gcgacatttg ccgctccgcc cgcgcgttgg tcgattcgtc cgattttcgc caccggcacg
    15541 ggggcttcgg gcgaaatacg ggacacatcg ccgaaccaat agcggtcgag catcacgtcg
    15601 ccgacaacca ggactttggc ttgcgcgaaa cgggatttga gggtttcttg ttggaacttg
    15661 gcggacatat tgcctccgtt gtattttttc agacggcctc agcctgtaag gccgtctgaa
    15721 aatcagtttg cttcaaggtt tgccacgcgg ttcacttcgc gcaatacggc taccggatcg
    15781 gcagcttggg ttacaggacg acccattacc aaataagtcg aaccggcagc caaggcttcg
    15841 gccggtgtca tgatgcggcg ctggtcatca ttattgccgg caacgtccaa gcggatgccg
    15901 ggcgtgacca agacaaaatc ctgtcccaat tcgcggcgca gcggcgcggc ttcttgggcg
    15961 gaacagacca cgccgtccaa gcccgaactt tgcgccagtt ttgccaagcg gatgacttgt
    16021 tcttcagggg cggtgttcaa accgatttcc gccaaatcgc tttgttccat gctggtcaac
    16081 acggttacgc cgattaagag cggcttcgtg ccgtatccgg caacggcttc tgctgcggct
    16141 tccatcatac ggcggccgcc cgatgcgtgc atatcgacca tccaaacgcc catatcggca
    16201 gcgactttgc aggcttgcgc gacggtgtgg ggaatatcgt ggtatttcaa atcgaggaaa
    16261 agtttgaaac cttgattgat taagctttct gccaaattgc gtcccgtcgc ggtaaacagc
    16321 tctttgccga ttttgatttg acacaatgtc gggtcaaggt tgcggacgaa tccgagcgtg
    16381 tctttttcgt tggaaaaatc aagggcgacg ataacggggg tgcgttgttg cggagtttgg
    16441 aagtcggaga ttaaaggatt catggtgttt tcgtttcggg ttttcagacg acctttatcg
    16501 tctttttcgc agtatagtgg attaacttta aaccggtacg gcgttgtctc gccttagttc
    16561 aaagagaacg cttctctaag gtgctgaagc acaaagtgaa tcggttccgt actatctgta
    16621 ctgcctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataac aacgtaaatt
    16681 caaaccgtag gtcgggcatt gatgtccgac ctacgcgaac tcaagcctcc gacaaactgt
    16741 ttcggataaa tttgaaccgg tacatcagcc gtcattcccg cgcaggcggg aatccattga
    16801 taaaactcag ttcatcagat ttaaacagcg attgcccaaa ttcagaaatg gattcccgcc
    16861 tgcgcgggaa tgacgccaaa tgattatttt tcggttgcaa cacgctgttt atcaaaaaca
    16921 ggtcgtctga aaacctgatt ttctgttttc agagtgcgaa gcctgcccgt gggtacgacc
    16981 ttttcgattt atttgcccaa tgcgcccaat acttcggctt tcacggcttc taccggctga
    17041 gtgccgtcaa ctttgatgta tttaggcgcg tgttcgcctt ccagtttgct gtaaaaatcg
    17101 accaaaactt cggtttgctc gtggtaaacg gcaaggcgtt ttttcacggt ttcttctttg
    17161 tcgtcgtcgc gctgaatcaa atcttcgccg gttacgtcgt ctttgccttc aactttgggc
    17221 gggttgtagg taacgtggta agtacggccg gaagccaaat gcacgcggcg gccgctcata
    17281 cggtcgacaa tcacgctgtc aggcacgtcg atttcaacga ctgcatccaa atccacgcct
    17341 gcttcaacca tcgcttcggc ttgtgccaat gtgcgcggga aaccgtcgaa caggaaaccg
    17401 tttttgcagt cgtcttgcgc gatgcgttct ttgaccatgc cgataatgat gtcgtcgcgc
    17461 accaagccgc cttcgtcaat gatttttttc gcttccaaac ccaagggcgt gccagcctta
    17521 atcgcggcgc ggagcatgtc gccggtagag atttgcggaa tgccgaacgc ggcggtgatg
    17581 aattgcgcct gagtgccttt gcccgcgccc ggcgcgccta aaagtaatgc tttcatgaag
    17641 tgatcctttg aaatgtgttt attggaatca aacagatggt ttacgattct acacggcttg
    17701 gcacaatagt tcaaaaacca actgcacaaa ctgccatttg caagcaaatg ttttgcatac
    17761 ctttgccgag tctgatattc acaagccggc aactttttgg gacaatcccc aaaatttcaa
    17821 aacatcacac cctaccaaaa aggaacatcc gtgaaacgaa tttttctgtt tttggctacc
    17881 aatatcgctg ttttggtcgt aatcaacatt gttttggcgg ttttgggcat caacagccgg
    17941 ggcggcacgg gcagcctgtt ggcgtattcc gccgtcgtcg gcttcactgg ttcgattatt
    18001 tcgctgctga tgtccaaatt catcgccaaa caatcggtcg gcgcggaagt tatcgacacg
    18061 ccgcgcaccg aagaagaagc ctggcttttg aacactgtcg aagcccaagc gcggcaatgg
    18121 aacctgaaaa cgcccgaagt cgccatctac cactcccccg aacccaatgc ctttgccacg
    18181 ggcgcatcga gaaacagctc cctgatcgcc gtcagcaccg gtttgctcga ccatatgacg
    18241 cgcgacgaag tggaagccgt attggcgcac gaaatggcac acgtcggcaa cggcgatatg
    18301 gttacgctga cgctgattca aggcgtggtc aataccttcg tcgtgttcct gtcgcgcatt
    18361 attgccaacc tgattgcccg aaacaacgac ggcagccagt cccagggaac ttatttcctg
    18421 gtcagcatgg tattccaaat cctattcggc ttccttgcca gcttaattgt catgtggttc
    18481 agccgacaac gcgaataccg cgccgacgcg ggcgcggcaa aactggtcgg cgcaccgaaa
    18541 atgatttccg ccctgcaaag gcttaaaggc aacccggtcg atttgcccga agaaatgaac
    18601 gcaatgggca tcgccggaga tacgcgcgac tccctgctca gcacccaccc ttcgctggac
    18661 aaccgaatcg cccgcctcaa atcgctctga gccgatttga aacaacaaaa aaaccgtacg
    18721 cgcaagcagt acggtttttt gtatatgctg tctgaaaaca tcaggtttcc ataccaacac
    18781 caaccgtatc catttccgca ccggatacgc cgcattgata taccctgata aaccgtgtcc
    18841 ggaca
//
LOCUS       DAWEWP010000028        18670 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWEWP010000028 DAWEWP010000000
VERSION     DAWEWP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18670
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.028"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            54..1064
                     /gene="waaF"
                     /locus_tag="WGN53_000687"
     CDS             54..1064
                     /gene="waaF"
                     /locus_tag="WGN53_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ0139702.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRYFAELGKHYSAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            1119..1565
                     /gene="smpB"
                     /locus_tag="WGN53_000688"
     CDS             1119..1565
                     /gene="smpB"
                     /locus_tag="WGN53_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ0139703.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            1931..3985
                     /locus_tag="WGN53_000689"
     CDS             1931..3985
                     /locus_tag="WGN53_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ0139704.1"
                     /translation="MYYETRSKQASKQASKQKFKKSFIISLFFSILYTSPLLAVDYVY
                     DNSKLTNEQIDRLKKLRDKSSEYWKQETYLLKPTNGSSQNSLKVPALYSKTHQFKNIN
                     NSKKISFYDKEYTEDYLVGFARGFGVEKRNGEEEKTLQQYFKDCVNTGNSNNDKCEQP
                     SFENSGPRLIKSDIFALAPQIKNSHINSEILSVGNYIEWLRPTLNKLPSWQEHLYSGL
                     DPFHYIEVTDNSHVIGQTISLDEFRLENSLWEPRWDSDVGELKTTNADIRFNTKSESL
                     LVKEDYAGGARFRFAYDLKDKVSETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQII
                     KVNGTADKHAFRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDISIYAQQAQAANTLFA
                     LRLNDKNSDIFDRTLPRKGLWLRVIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQN
                     ESNQLSVGLMSGQAEQRSTFRNPDTDNLTTGNVKGFGAGIYATWHQLQDKQTGAYADS
                     WVQYQRFRHRINTEDATERFTSKGITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQL
                     TYLGVNGKFSDSENAHVNLLGSRQLQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNK
                     PFGVEIDGERRVINNKTAIESQLGVAVKIKSHLTLQASFNRQTSKYHQAKQGALNLQW
                     TF"
     gene            complement(4166..5533)
                     /locus_tag="WGN53_000690"
     CDS             complement(4166..5533)
                     /locus_tag="WGN53_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0139705.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLIIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(5643..5924)
                     /locus_tag="WGN53_000691"
     CDS             complement(5643..5924)
                     /locus_tag="WGN53_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139706.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAAAT
                     EAPAAEAPTAEAAATEAPAAEAPAAEAAATEAPAAEAPAAEAPAAEAAK"
     gene            6397..6879
                     /locus_tag="WGN53_000692"
     CDS             6397..6879
                     /locus_tag="WGN53_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0139707.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            6966..7778
                     /gene="hpnC"
                     /locus_tag="WGN53_000693"
     CDS             6966..7778
                     /gene="hpnC"
                     /locus_tag="WGN53_000693"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ0139708.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(7849..8319)
                     /locus_tag="WGN53_000694"
     CDS             complement(7849..8319)
                     /locus_tag="WGN53_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ0139709.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHKIA"
     gene            8410..10251
                     /locus_tag="WGN53_000695"
     CDS             8410..10251
                     /locus_tag="WGN53_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ0139710.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTNPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEVGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANKP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            complement(10767..11963)
                     /locus_tag="WGN53_000696"
     CDS             complement(10767..11963)
                     /locus_tag="WGN53_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0139711.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPNHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            12314..12613
                     /locus_tag="WGN53_000697"
     CDS             12314..12613
                     /locus_tag="WGN53_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139712.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            12613..12798
                     /gene="ccoS"
                     /locus_tag="WGN53_000698"
     CDS             12613..12798
                     /gene="ccoS"
                     /locus_tag="WGN53_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ0139713.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            12788..14098
                     /locus_tag="WGN53_000699"
     CDS             12788..14098
                     /locus_tag="WGN53_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0139714.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRYKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            14256..14990
                     /gene="dnaQ"
                     /locus_tag="WGN53_000700"
     CDS             14256..14990
                     /gene="dnaQ"
                     /locus_tag="WGN53_000700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ0139715.1"
                     /translation="MIQRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            14987..15676
                     /locus_tag="WGN53_000701"
     CDS             14987..15676
                     /locus_tag="WGN53_000701"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ0139716.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTLGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIHPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            15708..16190
                     /locus_tag="WGN53_000702"
     CDS             15708..16190
                     /locus_tag="WGN53_000702"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ0139717.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            16267..16938
                     /gene="rpiA"
                     /locus_tag="WGN53_000703"
     CDS             16267..16938
                     /gene="rpiA"
                     /locus_tag="WGN53_000703"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ0139718.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLMMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            17007..17678
                     /locus_tag="WGN53_000704"
     CDS             17007..17678
                     /locus_tag="WGN53_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ0139719.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            17857..18603
                     /locus_tag="WGN53_000705"
     CDS             17857..18603
                     /locus_tag="WGN53_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0139720.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
BASE COUNT         4449 a         4250 c         5307 g         4664 t
ORIGIN      
        1 ttttccaacg gtttcgcggt aaaattctgc ctgtctgatt tcggtttctt gttatgtcca
       61 tcaaaatcct gattatttct cccagttgga taggcgactg cgtgatgacc cagcccttgt
      121 tccgccgttt gaaggaactt caccccggtt gcacgattga tgtgttcgca ccgaagtggt
      181 cgatggcggt gtttgagcgt atgccggaag tgaatgagat tcttgaaaat ccgttcggac
      241 acggtgcgtt ggagttgaaa cgccgctggc gggtcggtag ggatttgggg cggcgcggat
      301 acgatcaggt tatcgtgttg cccggttctt tgaaatcggc aatcatcgcg ctggcgacag
      361 gtatcggtaa aaggacgggt tatgtcggtg aaagccgtta ttttctgttg aacgatatac
      421 gcaggctgga taaggaacgt ctgcctttga tggtggacag atatacggct ctcgcgcatc
      481 cgagccagga ggattttgac gggcattcgg gattccccga gttttccatt gatgaacggc
      541 ggcgggaaat ttctgtcgaa acctttggct tggatattgg aaagcctgtt ctagcttttt
      601 gtccgggtgc ggaattcggg ccggcaaagc gttggccgac aaggtatttt gccgagttgg
      661 gcaaacatta ttcggcggcg ggttggcagg tttggctgtt cggttcgcaa aaagatgatg
      721 aaattgccga ggaaatcaac tgcctttcag acggtatgtg tgtgaattta tgcggaaaga
      781 ccgatttgtc gcaggcaatg gatttgctgt cgttggcgga cacggtcgtg tgcaacgaca
      841 gcgggctgat gcacttggcg gcggcattgg gcaggaaggt ggtggcggtt tacggttctt
      901 ccagcccgac gcatacgccg cctttgagcg acagggcgaa aatcgtcagc ctgcacttgg
      961 aatgttcgcc ctgttttaag cgggaatgtc cgctggggca taccgactgc ctcaacaggc
     1021 tgtatcccga gaagattgtg caggcggttg aagaggcggt atgatgggtt ctgttttcgg
     1081 gtttatgccg tctgacggcg gatattgata ggatgtttat ggctattgcg aacaataaaa
     1141 aagcatttca cgattttttc attgaagacc ggattgaagc cggtttggtc ttggaaggct
     1201 gggaagtcaa agcaatccgc gccgcgcgcg tacagcttaa agagagttat atctattgga
     1261 aaaaagacgc gttttatctg gtcggctgcc atattacggc tttgcccaca gcctcgacac
     1321 acattaaacc agatgccgta cgtccgcgca agctcttgtt gaaccagtcg gaaatcaaca
     1381 aattaatcgg caaaaccgaa cgtgccggct acaccatcgt gccgctggat ttgcattttt
     1441 cgcgcggaaa aatcaagatg gaaatcggtt tggcaaaagg taagaaacag cacgacaaac
     1501 gtcagagtat gaaagaggcg gattggaaac gcgagaaaca gcggttgatt aagcatacgc
     1561 gctgattgtg gaaacagtgc ttcagagggt atgggcaagg ctgccgcctg atgtgtaaac
     1621 gttctacacc taaaccagca tccgctttaa tcagcacagc atgatgcccc aaaccttcgt
     1681 caaaggtttg agcaatttga tgataaattg gcattagcca agggtaaagt gcggtcattt
     1741 ttcctgaaat ttttgtaaat aacgagggca attatacaca ttttcaaact ggcttgacaa
     1801 aatagcaaaa agcaaacgtt tacctttaat aaataagaaa taaatagaga aaaatgtgag
     1861 ctaaatgaca ttttttgttc agttttatgc taaaatccac gccccattta tttttacttg
     1921 acaaaggaaa atttattatg aaactcgaag caagcaagca agcaagcaag caagcaagca
     1981 gaagtttaaa aaatcattta ttataagtct atttttttct attctttata cctctccgct
     2041 tttggctgtt gattacgttt acgacaattc taaattaaca aatgagcaaa ttgatcgctt
     2101 aaaaaaactt agagataaaa gcagcgaata ctggaaacaa gaaacttatc ttctaaaacc
     2161 gacgaatggt agctctcaga actctctcaa agttcccgca ctatactcta aaacacacca
     2221 attcaaaaac atcaacaact caaaaaagat ttctttttat gacaaggaat acactgaaga
     2281 ttaccttgtt ggcttcgccc gaggtttcgg ggttgaaaaa agaaatgggg aagaagaaaa
     2341 aacactccag caatatttta aggactgtgt aaatactggg aattccaata atgataagtg
     2401 tgaacaacca tccttcgaaa actctggccc aagattaata aagagcgata ttttcgcctt
     2461 agctccacaa ataaaaaata gccatatcaa cagcgaaatt ttatctgtag gtaattatat
     2521 cgaatggctt cgcccaacac ttaataagct tcctagttgg caagagcatc tctattctgg
     2581 tttagatcca ttccactata ttgaggttac agacaactca cacgttatcg gacaaacaat
     2641 tagtttggat gaattccgct tagaaaattc tctctgggaa ccccgttggg attctgatgt
     2701 tggggaatta aaaacaacga atgcagacat ccgttttaat actaaaagtg aaagtttact
     2761 cgtaaaagaa gattatgctg gcggagctcg ttttcgtttt gcttacgatc taaaagataa
     2821 agtctctgaa acaccagttt taacttttga aaaaaatata actggcactt ccgacatcat
     2881 cttcgaaaat ccaatagacg atttaaaaag tctcgacggg catcaaatta tcaaagtaaa
     2941 tggcacagca gataaacacg cattccgtct ttctggcaaa caccaaaagg gaatttatac
     3001 gctttcttta caacaacgcc cagaaggctt ttttaccaaa gtgcaagaac gcgatgatat
     3061 ttcgatttat gcacaacagg ctcaagccgc caatacctta ttcgccttgc gtttgaacga
     3121 caaaaacagc gatatttttg accgcacttt accacgcaaa ggcttgtggt tgcgtgtgat
     3181 tgacggacat tccaaccaat gggtgcaagg caaaacagca cctgtagaag gctatcgtaa
     3241 aggtgtgcaa ctcggtggcg aagtattcac atggcaaaac gaaagtaatc aactttccgt
     3301 tggcttaatg agcggacaag cagaacaacg cagcactttc cgcaatccag atacagacaa
     3361 tcttacaacg ggaaatgtga aaggctttgg ggcgggcatt tatgccactt ggcatcagct
     3421 tcaggacaaa cagacaggtg cgtatgcgga tagctgggta caatatcaac gcttccgcca
     3481 ccgcattaac actgaagatg ctacagaacg ttttacttca aaaggtatta ccgcctcaat
     3541 tgaagcgggt tacaatgctt tattggcaga acacttcact aaaaagggca acagccttcg
     3601 tgtttaccta caaccacagg cacaattgac ttatttgggg gtaaacggaa aattcagcga
     3661 tagcgaaaat gcccacgtga atttacttgg ctcccgccaa ttacaaactc gggttggcgt
     3721 tcaagctaaa gctcaatttt cactctataa aaatatcgct attgaacctt tcgccgctgt
     3781 gaacgcgctt taccacaaca aacctttcgg tgtggaaata gacggagagc gtcgagtgat
     3841 aaacaataag acggcaattg agagccaatt aggtgtagca gtgaaaatca aatctcactt
     3901 aactttacaa gcctcattca accgccaaac aagcaaatat catcaggcta aacaaggtgc
     3961 gttgaatttg cagtggacgt tttaagttaa gttgtaacaa aataaaatcg gcagggaaat
     4021 tcactgccga tttttttatt ttaaaaatgc ggtcattttt cgtgtcattt ccaccaattt
     4081 tttaccgcac tcacgagctc gcgctaaacc gacaatcgga tctattgtcc ctttctttgt
     4141 tgtgtatgca aatcagaaat gccggttacg gaagcagttt gcccggattc ataatgttat
     4201 atggatcaag gtgttgtttg atgcttttca tcagggcgat ttcggcaggc gtgcgtactt
     4261 tgtccagcca ctgttttttg atgataccta tgccgtgttc ggcggcaatc gtgccgttgc
     4321 aggcaagaac gttgcgataa actgcgtcgt tgatgtcgtt ttcgtaacga tagacttcat
     4381 tgctgaggat ttcgggcagg aaagtattgt agtgcaggct gccgtcgcca agatgtccga
     4441 agcagacgat ttgtatgcct ttaaaattct gttccaaatc tttggcgcac cggcggacaa
     4501 agtcggcaac gcgtccgata ggaacggcaa tatcgtgttt gatgctggtg cccagtttgc
     4561 gttgggaggc ggagatgttt tcgcgcaatg cccacatatg gatacgttct tgttcgcttt
     4621 gtgccaatac gctgtcggta aatccttttt tataaagaaa ttcgacgagc cgatcatcaa
     4681 gattgctgtc gggtaatgag tcggtcaact caagtaaaat atgccattct gaatgtgtcg
     4741 gcagggggaa tttgctgaat tcggaagaca actcggcggc aaaacggccg atcagctcaa
     4801 aactgcatag gcgttcggca aagtgagctt gggtttcggt cagcaggcgg acggcggatt
     4861 cgatgtcggg tatgccgacc catgccgttg ccttgtccga gggacgggca aaaagcttga
     4921 gtgtggcggc agtgatgatg cccagtgtgc cttcgctgcc gataaacaga tggcgcaggt
     4981 cgtagccggt ggtgttttta tgcaggggat ggagatggga aaccagctcg ccgttgggga
     5041 ggacgacttc caaaccgata atcaggtcgc gcatcgtgcc gtaacgcaat acgttcaaac
     5101 ctccggcatt gcaggcgatg ttgccgccga tttgacagga gccttcgctg gcgagactga
     5161 gtgggaacag cctgtttgag gcttcggctg cctgttggac ggtttggagt acggaacctg
     5221 cttcgacggt tatgcagttg tctgacaaat tgatgctgcg gatgcggttg agtttggaaa
     5281 ggttcagcaa tacgccgttt tccgatactg ccgcgccgca caaaccggta ttgccgcctt
     5341 gcggcgtaac cggaatacgg tgttcgtaac aaaaacgcat aatggtttgc acgctttcaa
     5401 cgctgcgcgg ctgcaaaatg atgtctggtg cagacgtaaa gcggttgcgc tggtctttta
     5461 aaagcgtcgg agaaagttcg gcaatttcat ctgccggcag gagtcgggaa aattcggtat
     5521 gaaggtcagg catgtttgtc ggaagggtag ggagaagatt gaggaaggga atctgatgcc
     5581 gtctgaaaaa tcaaaaaagc aggatactga acgtatcctg cttttttgca agcggaaaat
     5641 gcttattttg cagcttcggc agcaggagct tcggcagcag gagcttcagc ggcaggtgct
     5701 tcggtagcgg cagcttcagc agcgggagct tcggcggcag gtgcttcagt agcggcagct
     5761 tcagcagtgg gagcttcggc ggcaggtgct tcagtagcgg cagcttcagc agcgggagct
     5821 tcggcggcag gtgcttcggt agcgggagct tcagcggctt tttcaccgcc gcaggctgcc
     5881 aggaccaaag acaacaaagc agcggcaaac agggattttt tcattttagt aacctttaga
     5941 accaatttaa attattgaaa caaataggtc tttatcgacc atactacaaa ccgggttgga
     6001 aattcatcgc tgaatttgcg taattatgca ctatggaata agggattgca atgtcgtaca
     6061 gatgggaaaa aggtttgaat gaaaagtttt ctggcgggcg gattgataaa tcctgtaaaa
     6121 cggttttgct gttaaaaatt cgatagggat aaatattata gagattaata taatttcagt
     6181 atcttgtatg atttattaag gcggattggg aaagtcggca gacaggtcgg aatggtttta
     6241 agaatgttgt aaaaaagtta caccgcaata aaataacaaa aatttacatt gaaatatgtg
     6301 ctgtacagtt tatgaacagg ttaacggtaa tcggccggcg cggcggttgt gttagaatgc
     6361 gcctgtaatc aatctgctgt tcctgaaagg acaaatatgg ctatcgtgaa aaattccgtg
     6421 gtttcgctgc attatgagat gtatgatgcc aacaatcagc ttttggacaa aaccgaagaa
     6481 ccgattgcgt atctgcacgg cggttacgac ggcattttcc ctttggtgga agaggcgttg
     6541 cacggtaagg atgccggcga tacggtcgat gtggcgctgt cgcccgacga tgcgttcggc
     6601 gagcaggatc cggagttggt ccgtattgaa gatgcgggcg tgttccctgt tgaagtcgaa
     6661 gtcggcatga tgtttgaagc cgacgatcct gaaaccggcg atgttgtcgt ctatcgtgta
     6721 accgatgttg ccgacggcaa ggcggtggtg gacggcaacc atcctttggc aggcatgaaa
     6781 atccgcttta aggctacggt tgaaagcgtg cgcgatgcat ccgatgagga aatcgcacac
     6841 ggtcatgtcc acggcccgca cggtcatcac caccactgat tgccggcagc ttggaggtat
     6901 ctgatgccgt ctgaaaaggg tttgttccgt ttcagacggc attttgtttg atggatggag
     6961 aaggtatgtc ggttgggcat tatgagaatt ttcccgtcgg ttcgctgatt ttgccgcgca
     7021 ggttgaggaa gccggttcac gcggtgtacg cgtttgcacg gacggcggac gatatggcgg
     7081 acgagggcag tatgccgtct gaagccaggt tggcggggtt ggagggtttg cagcgcgagt
     7141 tggacgtgtt ggcatcgggc ggccggtcgg cgcatccttt gattgcacga ttggatgccg
     7201 aggcggttgt gccgttcggt ttggatttgc agccgtttta tgatttgctt tctgcatttt
     7261 ctcaggatgt ggttaaaacg cggtacgcgc atttcggcga gctggccgat tattgtcggc
     7321 gttccgccaa ccctgtcgga cgcattatgc tggctttgta cgggaaaacg gatgcggtgt
     7381 gcgtggcgca aagcgacggt atctgtacgg ctttgcaact ggtgaatttt tggcaggatg
     7441 tggctgtgga ttggcaaaag ggcagggttt atatcccgca ggacgatttg ttgaaattcg
     7501 gtgtttccga aggacagatt gcggagggta gggcggatgc ggcgtttcag cggctgatgg
     7561 cgtatgagtg ccggcgcgca ttccgtatgc tgaaggcggg ttcgcctttg gcgcgcgaat
     7621 tgaaagggcg tatcggtttg gaactccgta tgattgtgtt gggggcgcag ttgattttgc
     7681 agaaactgga cgcgtgccga tacgatgtgt ttgcacaacg tccggttttg gataagaagg
     7741 attggttgat tatgttgaaa cgcgcgttgt ggaaatgatg ggggaaatga atgccgtctg
     7801 aaaggggttg ttccgcttca gacggcattt tgcaggcggt tggtatggtc aggcgatttt
     7861 atgcgggttc aacctggctt gatcttctgc acccaagccg atgagtttgc gcagtcgggt
     7921 catgtagaaa ttgacatcca aaccggtgcc gtagcgttgc gccgtccaca gtgtttccgc
     7981 cagtgcctcc atcatttcgt gttcggcttc cagccagccg cgtttggcgc acagggtgtc
     8041 gtggattgcg cgtatgccgt gcggctggtc tatgcccgcc tgttcttgga cggacaaatg
     8101 cagcgacata tgcaggaagg ggttgctttc gccgttttcg ggcagccagt cggtgtccag
     8161 atggtcttcg atgcgttcga gataacggtg gtattcggga tgggcttcga taatgcggag
     8221 ggctttctgt tccagtgcgc tcagttgcag cggattgagc cgctgctgcc acacgcgggc
     8281 gaaaaagcgg cggacatcgt gggtattgac atcgtacatg gcgggttcgg taagatgttt
     8341 atggaaatag gttcggataa tggcgggttt tacataaatt aaatcccacc gtcaacctta
     8401 ccatttataa tgcagacaaa ttatttgtct gaaacggatt cgattatgaa aaaactgatt
     8461 tgcctgttcg ccgtattttt gatgttgtgc ggacgagctt tcgcgctgga tgcgaacgat
     8521 ctgctgccgc cggaaaaggc attcgtgccg gagcttgccg ttgccgacga cggtgtgaac
     8581 gtccgtttca ggattgccga cggatactat atgtatcagg cgaaaatcgt cggcaagacc
     8641 aatccggcgg atttgttggg acagccttct ttcagcaagg gcgaagagaa ggaagacgag
     8701 tttttcggca ggcagacggt ttaccatcac gaagcgcagg ttgcctttcc ttatgcaaag
     8761 gctgtcggcg aaccgtataa attggttttg acctatcagg gctgtgccga agtcggcgtg
     8821 tgctatccgc ccgtggatac cgagtttgat atttccggca acggcactta ccatccgcaa
     8881 accgacgaac cggcatccgc caaagaccgc tttttgcagc cttcctctca aaacggcagc
     8941 ggggcgctgc cgcccccgaa gggggatgag ggcggcgaca gccgtttcaa gctgtcttgg
     9001 gatacgctca acgccaatct tttggcgttt tttctcgccg gtttgggtct gagttttacc
     9061 gcctgtatgt atcccctgtt gccgattgtt tccagtattg tggtcggcga caaaaaggcg
     9121 ggcaaggcgc gggcgtttgt gctgtccgtc gtttatgttc agggtttggc tctgacttat
     9181 acgctggtcg gcattgttgc cggactgacg ggcgcgctgc tgaccgtatg gttgcagcag
     9241 gcttgggtcg tgttggcggc atcggctttg atggtcgtct tggcactgtc tatgttcggg
     9301 ctgttcaaca tccagcttcc caacgccgtg cagtcgtatt ttcagaatca aagcagcagg
     9361 ctttcaggcg gtaaaatcgt ttccgtcttt attatgggca tattgtccgc gctgattgtc
     9421 gggccgtgcg tcgccccgcc gctggcattt gctttgggct acatcggtca gacgggcgat
     9481 gcggttttag gcggtttggc actttacact ttggcgttgg gcaccggcgt tccgctgatt
     9541 gccatcggca cgttcggcgg gcatatcctg cctaaggcag gcgattggat gaatgccgtc
     9601 aaatacgctt tcggctttat cctgcttgcc gtcgccgttt acctcgccac gccgcacttg
     9661 ccctattatc tcgtcgtcgc gctgtacacg ctgctgatgc tggttcctgc ctgtatgctg
     9721 ctggtcaacg gacgcaggca gaaacgccgt ccgaaagctg tggcattcgc attgggcggc
     9781 atattgctga taggcggcgc gtggttcggc tggcagggcg caaacggcaa aacgaccgcg
     9841 ctgcaccatt tcctgaccct caatccgccg gccgaagcag gcaaatcttc ggaacacgga
     9901 aaaatgtttg ccgatactgc cgcgctgaag gcagcgatgg atacggcgtt gaaagaacat
     9961 cccgacaaac ccgtcgtttt ggatttttat gccgactggt gcatttcctg caaagaaatg
    10021 gcggcttaca cgctcaatca gccggaagtg catcaggcag tcgatatgga acgctttttc
    10081 caaatcgacg taaccgccaa caagcccgaa catcaggcgt tgttgaaaga atacggtctg
    10141 ttcgggccgc cgggcgtgtt tgtcgtccgc tccgacggca gccgcagcga gccgctgctg
    10201 ggttttgtca aagcagacaa gtttatcgag tggtatgaac aaaaccgctg attccgatgt
    10261 ttcaaatgcc gtctgaagtt ctcagacggc atttttgtca gaaaggaaaa cggcaatatc
    10321 cactatacac acggaagcag tctaaagatg tgaaaaccgt gtgtataagt tagtttgcgt
    10381 attgaaatta aaagataaaa atacgatgat taaaaaatag gcataagaaa agataaacac
    10441 agccggtgtt tgccggtgtg tgtatctttt gtggaaaaac atcttaatgt catgtatttt
    10501 atggaaaata ataacatgat taaaaaatag tcaatcatta tagtggatta aatttaaatc
    10561 aggacaaggc gacgaagccg cagacagtac agatagtacg gcaaggcgag gcaacgccgt
    10621 actgatttaa atttaatcca ctataaggtt caggtaagcg gctgcaaccg tcaatgtctg
    10681 aaaagaggca gtgctgttta agcatggaaa gccggataaa aaaaactgcc tccgtttccg
    10741 gaggcagttc gtgtgcggat aaaaccttac agaccgctca aacgggcagt gtcgtgggca
    10801 atcatcagct cttcgttggt cggaatgacc acggcaacgg ctttgctgtc ggcagtggta
    10861 atcacgccgg cgttgccgaa gcgggctttc aggttggctt cttggtcgat gttcagaccg
    10921 aggaagccca agtagccgat cacgcgttcg cggatgatgt cggagttttc gccgatgccg
    10981 ccggtaaaga ccagtgcgtc caaaccgcct gcggcaaccg ccatactgcc gatgtatttg
    11041 gcaaggcggt agataaacat atccaaggcc aatttcgcgc ctttatgccc cttggcggct
    11101 tcttcttcaa tggtgcggca gtcgttggac aggccggaaa tgccgagcag accggatttt
    11161 ttgttcagca tttcagtgat ttgggcgatg gtcatattgg cgttttcggc gagaaagccg
    11221 aatacggaag gatcgatgtc gccgctgcgc gtacccatta ccagcccttc cagcggggtc
    11281 aggcccatac tggtgtcgcg cgattcgccg ttggcgacgg cggtaatgga cgcgccgttg
    11341 cccaagtggg caatgaccat acgcaggtct tttttgtctt tgccgaggaa gcgcgcggtt
    11401 tcgtcggcga cgaagcggta gctggtaccg tgcgcgccgt aacggcgcag gccgtatttt
    11461 tcatacaact cctgcggaac ggcgtatttg taggcgactt cgggcatggt ttggtggaag
    11521 gaggtatcga atacgacgac gttgggcagg tctttgaaaa tgctttgcgc ggcacgcagg
    11581 cccaagaggt gggcggggtt gtgcaggggg gcgagcggga tgcatttttc gatgccggca
    11641 atgacttcgt cgtcaacgag gatggattcg ctgtacagtt cgccgccgct gacgacgcgg
    11701 tggccgatgg cgccgatgcg gctgtcgagg ccgtgggctt tgagttcttc catcagggct
    11761 tcgaccgcgc cggtgtggtt gggatgtgcg gacagatcga ctttgtgttt ttcgccgttt
    11821 actttgaatg tgatgtaggc atcgggcagg ttgagttttt cggcaaggca gctgagcagg
    11881 acttcgccgc tgccgttatc caggaccgcg cctttgaggg acgagctgcc gcagttcaaa
    11941 accaagatca atttttggga cattttctta ctccggaaag tttcagacgg cattggaatc
    12001 ggacacggat actaaccgga ttcgtgccga atccgttttg ccttcctggg cgggaaagta
    12061 gtggggccgt ctgaaaaggt tgataaaaga agcaggctat tctagcaaaa aatctttgca
    12121 attgcttggc ttaatcgggc gtttgcgtga aaatggcgga agtcgggcat tttgacggaa
    12181 aaaaggggga tatgcggttt gccggtgttt tcggctgggg gagggagaat ttctttgcat
    12241 tcttcccgtc caaactggta cattcgccct gtttttagtt ggtgtcgtct gaaaaaaggc
    12301 gggtgggaaa tttatgcatt atttcagtat tcatgaccaa tcgggacggc atataggctt
    12361 tttcatcctg cttgccgacg acgaatcgga agcacggccg caaagcgggc gttttgccgt
    12421 caaacttgaa ggcggaatgg aaaatcatgt cctgtcgccg ttcggtcaga cggaaatccc
    12481 ccaatattgg cgcgtggtca aagaccgtat cgagctgttt tttgacgaag ctcccgtcgg
    12541 aactttgcgt aacgaatatc tgaccgtgtc ggggcagact tttgttttga acgatttgac
    12601 agggaatatg tgatggaaag catgtttata ttggtgccca tcagcattat tttggcgttt
    12661 gtcatcggct ggtttttttg gtggtcgggc aaaaacgggc agtttgacga cttggaaggg
    12721 ccggcgcacc gcatcctgat ggatgatgat tcgacaagta aactgattga aaaagagagg
    12781 gaaaacgatg tacgctaaaa aaggcggttt gggactggtt aaaagccgcc gtttcgcacc
    12841 tcttttcgct acgcagtttc tcggcgcgtt caacgacaat gtgttcaaaa ccgcgctgtt
    12901 tgtgatgatt gggttttacg gtttggggca aaacggcttc ctgcctgccg gacagatgtt
    12961 gaacttgggc gcgttgttgt ttattttgcc gtatttcctg ttttcctcgc tgtcggggca
    13021 gttgggtaac aaattcgaca aggccgtttt ggcgcgttgg gtcaaggtgc tggaaatgat
    13081 cattatggcg gtggcggcat acgggtttta tatccggtct gccccgctgc ttttggcgtg
    13141 tctgttttgc atgggcgcgc aatcgacgct gttcgggccg ctgaaatacg ccatcctgcc
    13201 cgattatctc gacgacaaag agttgatgat gggcaacagc ctgattgaat cgggtacgtt
    13261 tgtcgccatc ctgttcggtc agattttggg aacggcggtg gcaggtgtac cgccttatat
    13321 tgtcgggata ctggttttgc tggtcgccgt cggaggcacg gtcggcagcc tgtttatgcc
    13381 gtccgtcccc gccaaggctg ccgataccca aattgagtgg aatattgtcc gtggcacaaa
    13441 atccctgctt cgtgaaacgg tgcggtacaa acccgtcttt accgctatta tcggtatttc
    13501 gtggttttgg tttgtcggcg cggtttatac cacgcaactg ccgaccttta cccaaatcca
    13561 tctgggcggc aacgacaatg ttttcaacct gatgcttgcc ctgttttcca tcggtattgc
    13621 cgccggttcg gtactgtgtg ccaagttcag cagggaacgc ctgatgttgg cttgggtaac
    13681 ggttggtgcg ttgggtttga cggtttgcgg cctggttttg gtgtggctga cgcacggaca
    13741 ccgttttgaa gggctgaacg gcattttttg gtttttatcg caaggatggg catatcccgt
    13801 gatggcggtg atgacgctga tcggcttttt cggcggattt ttctccgttc cgctctatac
    13861 ctggctgcaa accgccagta gcgagacttt ccgcgcccgc gccgttgccg ccaacaatat
    13921 cgttaacggt atttttatgg tttccgccgc cgttttgagc gcggtgttgc tgtttttgtt
    13981 tgacagcatt tccttgttgt atctgattgt cgctttgggc aatattccgt tgtcggtatt
    14041 tttgattaag cgcgaaaggc ggtttttagg cgcggcggca atcaggaaaa aaccttgaat
    14101 gccgtctgaa aaaattgtcc ccaaaataca caatccgcct gcccaatgtt gtgaacacac
    14161 tgtgaataag tgggtttgtg cattgaaatg aaaacagaaa aatatattgc ttaaaaaata
    14221 gtcagcagat ggaaaatttg aagaaagaag cagacatgat tcaacgccaa atcatcctcg
    14281 ataccgaaac caccggtctg tatgccgacg gcggcgaccg cttggtcgag tttgccggtt
    14341 tagaaatggt caaccgccaa atgaccgaca aaaacctgca cctctatgtc caccccgagc
    14401 gcgatatgcc tgaagaggcg gcgagggtgc acggtctgac gatagaggtt ttggaaggga
    14461 aaaacgcacc gccttttgca gaggtcggca ggcagattgc cgatttcctg cgcggtgcgg
    14521 aactgattat ccacaatgcc aaattcgacg tgggcttcct caatatggag ttccgccgga
    14581 tggggttgcc gaccgttgag gaactcggct gtaccgttac cgataccctg gcgatggcgc
    14641 gcgaaatgtt ccccgggcag aaagccagcc tggatgccct gtgcaaccgt ttttccgtcg
    14701 accgcagcaa gcgcgtcctg cacggcgcat tgatcgactg cgaacttttg ggcgaagtct
    14761 atcttgctat gacgcggcgg cagttcgacc tgatgggtgc ggccgcagag gaaaaaatgg
    14821 aaaccaaacc cgtggtgcat accgaaacca aacgcagcgg caaactgaaa gtcatcaggg
    14881 cggacgaaaa cgagttggcg gcgcacgggc aatatttgga cggcttgggc gaagcctgta
    14941 tctggcgcaa ggaagccgtg ccgtctgaaa acggcgggac ggacgcatga agcgcaaaaa
    15001 tatcgcgctg attcccgccg ccggcatcgg ggcgcgtttc ggtgcggaca aacccaagca
    15061 atatgtcgaa atcggaagca aaaccgtttt agaacatacg cttgggattt ttgaacggca
    15121 tgagggcgtc gatttgaccg tcgttgtcgt ctcgcccgaa gacacgtttg ccgataaggt
    15181 tcagacggca tttccacagg ttcgggtgtg gaaaaacggc ggacagaccc gcgccgaaac
    15241 cgtccgaaac ggtgtggcaa aattgttgga aaccggtttg gcggcggaaa ccgacaatat
    15301 tctggtacac gatgccgcgc gttgctgcct gccgtctgaa gctttgacgc ggttgataga
    15361 acaggcgggc aacgctgccg aaggtgggat tttggcaatt cccgttgccg atacgctcaa
    15421 gcgcgcggac ggtgggaaca ttagtgcaac cgtcgagcgg acgagccttt ggcaggcaca
    15481 aacgccgcag cttttccgcg ccgggctgct gcaccgcgca ttggctgcgg aaaacttgga
    15541 cggcattacc gatgaagcgt ccgccgtcga aaaattgggc atccaccctt tgctggtgca
    15601 gggcgacgcg cgcaatttga aactgacgca gccgcaggac gcatacatcg tcaggctgct
    15661 gctcgatgcc gtctgaactt caattcaaaa catcaggaac acaaacgatg acgaacatcc
    15721 gtatcggaca gggctacgat gtccaccaac tgaccgaagg cagaaagctg atacttggcg
    15781 gagttgaaat cccatttgaa aaagggctgc tcggacactc cgatgccgat gcgctgctgc
    15841 acgccgttac cgacgcgctg ctcggtgcgg caggtttggg cgatatcggc agccatttcc
    15901 ccgacaccgc cgcagagttc aaagatgcgg acagccgcgt ccttttgcgt gcggcgtatc
    15961 aaagcgttca ggcgcagggt tggcaggcgg tcaatgtcga tacgaccgtg attgcacaga
    16021 aacccaaact cgcgccgcat attccgcaaa tgcgtgccaa catcgccgcc gatttgggca
    16081 tagacatttc gtgcgtcaac atcaaaggca aaaccaacga aaaactcggt tatctcgggc
    16141 gcatggaagg catagaggcg caggcggcgg tattgctggt acggatttga acgcggcggg
    16201 caggacgtgc aaaaaaatgt taagatggcg gctgtttgtt gatacggttt cccaaaggac
    16261 aaaaatatga cgacacaaga cgaactcaag cgcatcgccg ccgaaaaagc agtcgaattc
    16321 gtacccgaaa acgaatacat cggcatcggt accggctcga ccatcaactt tttcatcgaa
    16381 gccttgggta agagcggcaa aaaaatcaaa ggcgcggtat ctacttccaa aaaatccagt
    16441 gaactgcttg cccaatatga tattcccgtc gtttccctga acgaagtatc ggggttggcg
    16501 gtctatattg acggtgcgga tgaagtgaac cacgccctgc aaatgattaa aggcggcggc
    16561 ggcgcacacc tcaacgaaaa aatcgtcgcc agcgcatcag aaaaattcgt ctgcattgcg
    16621 gacgaaagca aatatgtttc acgtttgggc aaattccctc tgcccgtgga agtcgtcgaa
    16681 agcgcgcgtt cccttgtttc gcgcaaactg ctcgctatgg gcggacagcc tgaattgcgt
    16741 atcggctaca ccacttttta cggcaaccaa atcgtcgatg tccacggctt gaatatcgac
    16801 aaaccgctga tgatggaaga cgaaatcaac aaaatcaccg gcgtactcga aaacggaata
    16861 ttcgcccgcg atgccgccga tgtgttgatt ttggggacgg aagagggtgc gaaagtcatc
    16921 tatccctgcc aagggtaatc ctgacgttaa gatgccgtct gaaatccggt tcagagatgt
    16981 tcagacggca tcggcatttg aaaaatatgc agattaaaaa aatcatgaaa tggcttcccg
    17041 tcgccctgtc gcttttgggt gcgttgggtt atacggggta cggcagcgag gcggtgcgga
    17101 cggcggttgc cgtactcgac gtactcggcg cggcagggga cgcgggttcc gacgcgcccg
    17161 cccgccgccg agcatcggcg aaatccggcc accgctacac aggcacggtg tccaaagtct
    17221 atgacggcga cacccttcac gttatcgacg gcgacggcgc gaaacacaaa atccggatgg
    17281 cgtatatcga cgcgccggag atgaaacagg cttacggcac gcgttcgcgc gacaacctgc
    17341 gcgcggcggc ggaaggcagg aaagtcagcg tgcgcgtgtt cgacaccgac cgctaccagc
    17401 gcgaagtggc gcaggtttct gtcggcaaaa ccgatttgaa cctgatgcag gtgcaggacg
    17461 gggcggcgtg gcattataaa agttatgcta aagaacagca ggataaggcg gattttgccg
    17521 attatgccga cgctcaaatt caggcggaaa gggaacgcaa aggattgtgg aaagctaaaa
    17581 atccgcaagc gccgtgggcg taccgccggg caggcaggag cggcgggggc aataaggatt
    17641 ggatggatgc cgtgggcgaa tggttgggca tttggtaaag acagcgcacg gctttgaacg
    17701 gcaaatgccg tctgaaaacc gcgcgccctg catttttgcg gagacgcgca accgccgtct
    17761 gccgcgataa aaaagttcag gcggcggttt ttacggtaaa atggcggatt attttgcaac
    17821 ggcggcagtt ccgccggttt ttgatacgga cggtatatgg attttcgttt tgacattatt
    17881 tacgaatacc gctggatgtt cctttacggc gcactgacga ccttggggct gacggtcgtg
    17941 gcgacggcgg gcggttcggt attgggtctg ttgttggcgt tggcgcgcct gattcacttg
    18001 gaaaaagccg gtgcgccgat gcgcgtgctg gcgtgggcgt tgcgtaaagt ttcgctgctg
    18061 tatgttacgc tgttccgggg tacgccgctg tttgtgcaga ttgtgatttg ggcgtatgtg
    18121 tggtttccgt ttttcgtcca tccttcagac ggcattttgg tcagcggcga ggcggcaatc
    18181 gcgctgcgtc gcggatacgg gccgctgatt gccggttctt tggcactgat cgccaactcg
    18241 ggggcgtata tctgtgagat tttccgcgcg ggcatccagt ctatagacaa aggacagatg
    18301 gaggcggcgc gttctttggg gctgacctat ccgcaggcga tgcgctatgt gattctgccg
    18361 caggcattgc gccgcatgct gccgcctttg gcgagcgagt tcatcacgct cttgaaagac
    18421 agctcgctgc tgtcggtcat tgctgtggcg gagttggcgt atgttcagaa tacgattacg
    18481 ggccggtatt cggtttatga agaaccgctt tacaccgtcg ccctgattta tctgttgatg
    18541 acgactttct taggctggat attcctgcgt ttggaaaaac gttacaatcc gcaacaccgc
    18601 tgattggagt catatcaaca aaaagctgcc cgggtattca tttgaggcag cttttttgtg
    18661 gcaaatttat
//
LOCUS       DAWEWP010000029        17476 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWEWP010000029 DAWEWP010000000
VERSION     DAWEWP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.029"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..138
                     /locus_tag="WGN53_000706"
     CDS             <1..138
                     /locus_tag="WGN53_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ0139721.1"
                     /translation="VVAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYH
                     F"
     gene            complement(112..321)
                     /locus_tag="WGN53_000707"
     CDS             complement(112..321)
                     /locus_tag="WGN53_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139722.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKCA
                     GKGLMPSEGSDGIGIGESEVVAHAQ"
     gene            complement(339..896)
                     /locus_tag="WGN53_000708"
     CDS             complement(339..896)
                     /locus_tag="WGN53_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0139723.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(972..2606)
                     /locus_tag="WGN53_000709"
     CDS             complement(972..2606)
                     /locus_tag="WGN53_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ0139724.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2680..3009)
                     /locus_tag="WGN53_000710"
     CDS             complement(2680..3009)
                     /locus_tag="WGN53_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139725.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPSVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRTPPHSRRFLA
                     LLCSVAL"
     gene            complement(3009..4115)
                     /gene="serC"
                     /locus_tag="WGN53_000711"
     CDS             complement(3009..4115)
                     /gene="serC"
                     /locus_tag="WGN53_000711"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ0139726.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGATVVIIRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4480..4911
                     /gene="rimP"
                     /locus_tag="WGN53_000712"
     CDS             4480..4911
                     /gene="rimP"
                     /locus_tag="WGN53_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ0139727.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4939..6441
                     /gene="nusA"
                     /locus_tag="WGN53_000713"
     CDS             4939..6441
                     /gene="nusA"
                     /locus_tag="WGN53_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ0139728.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6453..9341
                     /gene="infB"
                     /locus_tag="WGN53_000714"
     CDS             6453..9341
                     /gene="infB"
                     /locus_tag="WGN53_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ0139729.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKTAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLHIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
     gene            complement(9553..10947)
                     /locus_tag="WGN53_000715"
     CDS             complement(9553..10947)
                     /locus_tag="WGN53_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ0139730.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGVDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(11021..12361)
                     /locus_tag="WGN53_000716"
     CDS             complement(11021..12361)
                     /locus_tag="WGN53_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ0139731.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            12504..13130
                     /locus_tag="WGN53_000717"
     CDS             12504..13130
                     /locus_tag="WGN53_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ0139732.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYVVMGWMVLAVMKSLTASLPLAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13194..14612)
                     /locus_tag="WGN53_000718"
     CDS             complement(13194..14612)
                     /locus_tag="WGN53_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0139733.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            14939..15667
                     /locus_tag="WGN53_000719"
     CDS             14939..15667
                     /locus_tag="WGN53_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ0139734.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15726..16214)
                     /locus_tag="WGN53_000720"
     CDS             complement(15726..16214)
                     /locus_tag="WGN53_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ0139735.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAVYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(16972..17436)
                     /locus_tag="WGN53_000721"
     CDS             complement(16972..17436)
                     /locus_tag="WGN53_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ0139736.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         4590 a         4891 c         4402 g         3593 t
ORIGIN      
        1 gtcgtcgccg gtgtcggttt cgacatcacg cccaagctga ccctggacac cggataccgt
       61 taccacaact ggggacgctt ggaaaacacc cgcttcaaaa cccacgaagt ctcattgggc
      121 atgcgctacc acttctgatt ccccgatacc gatgccgtct gaaccttcag acggcatgag
      181 acctttgcct gcgcacttgg tgcgctggtc gcctccgaac atggcgcgac acccgacatt
      241 tccgccgaac gcatcgggcg tttcatgaat ccggtttaaa acgcatggaa aaatgccgtc
      301 tgaaagcctt tcagacggca ttgtgcttga gattccgttt accaatggct gacaaacgct
      361 tccaaatcgg tattcttggg cttatgcact tcctctgtcg gcgtgccgac catcatcagc
      421 ccgatgattt tatccttatc cgcacaaccg aaagcctccc gcaacaaggg gctgttgacc
      481 cacatccccg taatccagac attgtcgaat ccctgagccg ttgccgccag ttgcagcgca
      541 tacgccgcac aacccgccgt cagcatctgc tcccattccg gtttcggctt aggcacatca
      601 cgattcggcg caaacgttac cccgatgacc atcggcgcca tattgcccac tttttccgcc
      661 tttttcatcg catcgtcgcc gaaattcaat tcggcaaccg tttgctttaa cacatcgcga
      721 aaacgttgca atcctacctc gccttgaatc acggtaaaac ggaaggggcg catattgccg
      781 tgatcgggaa cttgggttgc cgcctgaaat atttgttcca actccgccgc atcgggggcg
      841 gggggcttca gctttttgga agatcggcgg ttcgtcaata attttaaagc atccatatcg
      901 ttatactccg gtcatcggtc gggtgttcgg acacatatgc cgtccgaagg cttcagacgg
      961 catatccggc atcagcgcgg acggcggcag gctgccaata tatccatttc cttccgatag
     1021 gtttggctat tggaaatgtc catcagcccc aataccgtgc tgaaatagtg gtcgtgcgaa
     1081 tattcgtttt ccgccgcttt ttgtttgagg cattggaaat ctatgccccc gtgttggcgg
     1141 aaggctttgg aaaaccacat aaccatcggg atatgcgtct gcccggaagg cgcgatggcg
     1201 taaggcgcgg cgtgcaggta catcccgttt tcgcccaaac tttcgccgtg gtcggaaaca
     1261 taatgcacca cgctttccaa atcgtcgcgg ttttcaagtt tgcggataac cttgtcgata
     1321 aactggtcca catacaaaac cgtattgtcg taagtgttga ccagcgtggc gcgggtgcat
     1381 ttgttgattt cgttggtgtc gcaggtcggc gtgaatttgc gttcggcttc ggtatagcgt
     1441 tcgtaatacg tcggcccgtg gctgccgatg gtatgcagga ttaaaaccgc gtctttatcg
     1501 tttttgttga ggacttcgtc gaacttagtc agcaggatat tgtcgagaca ctcgccgttg
     1561 cggcagtatt cgggcaggtt gagcgaggta acgtcggtat tcggcacttt gccgcacacg
     1621 cccttgcagc cggaatcgtt ttccaaccag gtaacttcca cgccggcgcg ctgcacgatg
     1681 tccagcaggt tgtcttggtg ttcggctttg atttcgtcat aatccgtgcg gtcgaaggtt
     1741 gagaacatac acggcaggga gtgcgcggtc gatgtgccgc agcttctgac ctgcgggaaa
     1801 ttgacaattt catcgccgcg cgcggcaagc agcggcgtag tttggcggct gtaaccgttc
     1861 aaaccccagt tggcggcacg cgtggtctcg cccacgacca gcaccacgaa acggcgcagg
     1921 ctgccggccg gccggttttg cacgaccgcc atatccaatt gcgtataagg aatattggaa
     1981 cgcttccaat ctttgtattt cgacacgccc gcgccgatga aattagatgg cacaatcaga
     2041 tgggttactg atttattgtt gcggaaaaac gaggcgtaat cctgatattg caacattgcg
     2101 atgcccaacg cgcacaaaaa ggaaacggcg gcaagcacaa ggcgcgtcaa aagctcctta
     2161 taccaaacgc ggtatttaac cttgacggcg atatacgcca gcgcgggcaa tacgcccaaa
     2221 catacaatcc acagcacata gcccggcgta atcaggcgcg cgctttcggc agccgtagtt
     2281 tgcaagacat tattcaacat cgacttgttg aaatagatat tgaaaaatat ttcttggtaa
     2341 gacaccgccg cactgataac caatatcaac ggaatcaata ccttatgcac gaaaggcagg
     2401 gcaatgacgt gaaaaacgaa attacttaaa aaaaacagca ccaccggcat cgtatagagg
     2461 aagatatccg ccccggtgcc gttaaaagga tgaagctcga caactttggc aaaaaaggcg
     2521 taattcaata ccagcgagga atacagggaa aggaaggcaa tcagcgcgga agagccgagc
     2581 ttcggcctca ggttcggttt tatcatttgg aatgtgtcgg ataagggttg gaaaaggcat
     2641 ccggcatttg gaatccggat tattgaaaaa gattcttaat tataaggcaa cggagcaaag
     2701 cagggcaaga aaacggcggc tgtgcggggg ggtccgcccg ccattcaaac gtccggcaga
     2761 cataaaaaca tcgtaagcaa gattcgaacc ggtctgcaac cgcccctgcc agaaaaacgg
     2821 gcaaagcatt tcatattgga aaaacccggc cgcgccgccg acgggacagt ccggcacaaa
     2881 cagcatcacg ctcggattga aaaggacgga tagccgccgg cagcacaatc ttaccacctc
     2941 caaaacgccg ccggagaacg ccgaaacagc cgatgccgtc tgaagccgct tcagacaaca
     3001 tcgggacatc aaccgtaacg ccgttggaaa tcgcgcataa aatccgccaa agcccgcacg
     3061 ccttcaagcg gcatcgcatt ataaatgctg gcacgcatac cgccgacggt tttatagccc
     3121 ttaagcaggc acaagccctg caattcggct tccagcacaa aacggcggtc aagctcctca
     3181 tcccccgttt ggaacacgac attcatttta gaacgcgcat tcggacggat acggttgata
     3241 tagaaaccgc cgctgccgtc tatcgtctca tacaaggttt gcgccttcag ccgattgacc
     3301 gcttcaattt ttttcacacc gccctgcgcc tgcagccagc ggaacaccag ccccgacata
     3361 taaatcgcgt aagttgacgg cgtgttgtac ataccttcgc ggttcaaatg cgaacggtag
     3421 ttgaacacat cgggaatatc gttcggacaa cgctcgagca aatcctcacg gataatgacc
     3481 acggtcgcac cggccgggcc gatgtttttc tgtgcgcctg cgtaaatcag tccgtagtcg
     3541 gcaacatcaa actcgcgcga caaaatctcg ctggacatat cacacaccag cggcggcatg
     3601 ccttctgaaa ggcgcggcac ttcacggtat tgcagcccgt tgaccgtttc attgacggca
     3661 aaatggacaa acgccgaatc gggtgcaaca tcccacgttt ccacaggcgg caggtcgaga
     3721 tagtcgaact gctcgccgcc atgcgccgcc aaacggattt ccgtatcggt caaacggctc
     3781 atctgttcat aagcgatacg gctccagttg cccgttacca ccgcgtcggc agtgcggaaa
     3841 ccgtgtgcca gattcatggc tgccatatta aattgggttg ttgctccacc ctgcagaaac
     3901 aatatcttat agttgtcagg cactttcaaa agctgcctca aatcctgttc cgcatgatgc
     3961 aggatgctca aaaacatttc cgaacggtgg ctcatcgcca tcacaggaaa acccgtaccg
     4021 ttgtagtcca acatttcctg ccgcgccgtt tccaacacgg cttcgggcaa tacggcaggg
     4081 ccggcggaaa aattgtaaat cggataaaga gacataatgc agccttgatt ctgaacaata
     4141 acccgcccga ttttaggctt gcagcaggct ttgtgcaagg atgggaaata cctgtcctcc
     4201 gccccgcttc atgccgcccg aacacggaaa acaacatcaa tatattgatt tacaaatata
     4261 aaaatcatgc tagcgacaaa tagatacatt tgttttgtca acaatattca cgatttccca
     4321 ttacaaacct cccttacacc cgcttttttc cgtcccaaaa acacaaaata aatcaacact
     4381 ttcatttctc cgcaaaagcg gttataatca cgccgatttt tcaactttga cgaaaaatgg
     4441 ccacggggcc atttttttat tatgtacata gggagcagca tggatatcca aaccatcctc
     4501 gaaaaaaccc tgcccggcct gggctacgaa ctggtcgatt tcgaactgac cgcgcaagga
     4561 acattgcgcg tgttcatcga caaagaaagc ggcattaccg tcgaagactg cgcaaccgtc
     4621 agcaaccact tgagccgcgt cttcatggtt gaagacatcg actacaaaaa cctggaaatt
     4681 tccagccccg gactcgaccg ccccttgaaa aaagccgccg acttcgtgcg ctttgccggt
     4741 cagaatgcca aaatcaaaac ccgcctgccg atagacggtc agaaaaactt tatcggtaaa
     4801 atcgaaggct gcgaaaacga taccgttacc gtatccttcg acggcaaaac cgtacaaatc
     4861 gaattgggca acatcgacaa agcccgtctg cgccccgaat tcaaattcta aaacacaaca
     4921 atattggaga tgttcaaaat gagtcgtgaa atgttacagc tggcagaagc actggcaagc
     4981 gaaaaaaacg ttgatgcgga agtcgtcttc caagcactgg aattcgccct gtctaccgcc
     5041 gccaagaaaa aggcagaccg cgaacacatg gacgtgcgcg tccaaatcaa ccgcgacacc
     5101 ggcgaatacc aaaccttccg ccgctggctg attgtcgccg atgaagacta tacctatccc
     5161 gatgtcgaaa aaaccatcga ggaaatccaa gaggaaattc ccggcactac catccaaatc
     5221 ggcgaatact acgaagagca gctgcccaac gaaggcttcg gccgccaagc cgcgcaaacc
     5281 gccaaacaaa tcatcctgca acgcatccgc gatgccgagc gcgagcagaa tctgaacgag
     5341 tttctcgccg tcaaagaaga catcgtgtcc ggcacggtca aacgcgtcga acgccacggc
     5401 atcatcgtcg aagtcgttgc cggcaaactg gacgcgctga ttccgcgcga ccaaatgatt
     5461 ccgcgcgaaa acttccgcag cggcgaccgc atccgcgccc tcttcctgcg cgtcgaagaa
     5521 atcggcaaca ccggccgcaa acaagtcatc ctgagccgta cttccggcga tttcctcgtc
     5581 aaactgtacg ccaatgaagt acctgaaatt gcagacggca tgcttgaaat ccgcgctgtc
     5641 gcccgcgacc cgggccagcg tgccaaagtc gccgtcaaag ccaacgacca gcgcatcgat
     5701 ccgcaaggca cctgtatcgg cgttcgcggt tcgcgtgtca atgccgtcag caacgaattg
     5761 tccggcgagc gcatcgacgt tgtcctttgg tcgcccgagc ctgcccaatt cgtcatgagc
     5821 gcgctctcac ccgccgaagt cagccgcatc gtcatcgacg aagacaaaca cgccgtcgat
     5881 gtcatcgttg ccgaagacca gctcgcgctc gccatcggac gcggcggtca aaacgtgcgc
     5941 cttgcttccg acctgactgg ctggcagctc aacatcatga cttccgccga ggcagacgaa
     6001 cgcaatgcgg cagaagatgc cgccatccgc cgcctgttta tggatcactt gaacgtggac
     6061 gaagaaaccg ccgacgtact ggttcaggaa ggttttgcaa ccttggaaga agtcgcctat
     6121 gttcctgccg ccgaactgct tgccattgaa ggatttgacg aagaaatcgt cgatatgctc
     6181 cgcaaccgcg cccgcgatgc catcctgacc atggcgattg ccgccgaaga aaaactgggc
     6241 gaagtgtccg acgatatgcg caacctcgaa ggcatagatg ctgatatgct ccgcagcctt
     6301 gccgaagcag gcattactac ccgcgacgac ttggcagagc ttgccgtgga cgaactgatt
     6361 gaaatcaccg gtgtaaacga agaaaccgca aaagccgtca tcctgaccgc acgcgaacac
     6421 tggtttaccg aagacaaata aagggggtac agatgagtaa cacaaccgta gaacaatttg
     6481 ccgccgagct gaaacgcccc gtcgaagacc tgttgaaaca gttgaaagaa gccggcgtca
     6541 gcaaaaacag cggcagcgat tccctgacgc tggacgacaa acagcttctg aacgcctacc
     6601 tgaccaagaa aaacggcagc aacggcggca ccatcagcat ccgccgcacc aaaaccgaag
     6661 tcagcaccgt tgacggcgta aaagtcgaaa cacgcaaacg cggacgcact gtcaacattc
     6721 cttctgccga agagttggca gcacaggtaa aagccgccca aacccaagcc gcacctgtcc
     6781 ggccggagca gacggcagaa gacgcggcaa aagcccgagc cgaagctgcc gcacgtgcag
     6841 aagcccgtgc caaggcagaa gcggaagcgg caaaactgaa agcggcaaaa gcaggcaaca
     6901 aagccaaacc tgccgcgcag aaacccaccg aagcaaaagc cgaaaccgca cccgttgcgg
     6961 cggaaaccaa acccgccgaa gaaagcaaag cggaaaaagc ccaagccgac aaaatgccgt
     7021 ctaaaaaacc cgccgagccc aaagaaaaag ccgccaagcc gaaacacgag cgaaacggca
     7081 aaggcaaaga tgccaaaaaa ccggcaaaac ctgccgcacc tgccgtgccg caacccgtgg
     7141 tcagcgcgga agaacaggcg caacgcgatg aagaagcacg ccgcgccgcc gcacttcgcg
     7201 cccaccagga agccctgttg aaagagaaac aggaacgcca ggcacgccgc gaagccatga
     7261 aacaacaggc agaacaacag gcaaaaaccg cacaggaagc caaaaccggc agacagcgtc
     7321 ccgccaaacc tgccgagaaa ccgcaggcag ccgcgccggc cgtcgaaaac aagcctgtca
     7381 atccggcaaa agcgaaaaaa gaagaccgcc gcaaccgcga tgacgaaggt caaggccgca
     7441 acgccaaagg caaaggcgga aaaggcgggc gcgaccgcaa caatgcacgc aacggcgacg
     7501 acgagcgcgt acgcggcagc aaaaaaggca aaaaactcaa actcgagccg aaccaacacg
     7561 ccttccaagc accgaccgaa cccgtcgtac acgaagtttt ggtgcctgaa accattaccg
     7621 ttgccgactt ggcacacaaa atggcagtca aaggcgtgga agtggtcaaa gccctgatga
     7681 agatgggcat gatggttacc atcaaccaat ccatcgacca agacaccgcc ctgattgtcg
     7741 tcgaagaact cggacacatc ggcaaacccg ccgccgccga cgatcccgaa gccttcttgg
     7801 acgagggcgc ggaagcggtg gaagccgaag cattgccgcg tccgcccgtc gttaccgtca
     7861 tgggccacgt cgaccacggc aaaacctcgc tgctggacta catccgccgt accaaagtgg
     7921 tacagggcga agcaggcggc attacccaac acatcggtgc ataccacgtt gaaaccccac
     7981 gcggcgtgat taccttcttg gatactccgg gtcacgaagc gtttaccgcc atgcgtgcgc
     8041 gcggtgcgaa agcaaccgat atcgtgattc ttgtggtcgc cgccgacgac ggtgtgatgc
     8101 cgcaaaccat cgaagcgatt gcccacgcca aagcggcagg cgtaccgatg gtggttgccg
     8161 tcaacaaaat cgataaagaa gccgccaacc cagagcgtat ccgccaagag ctgaccgcac
     8221 acgaagtcgt gcctgacgaa tggggcggcg atgtacagtt tatcgacgtt tccgctaaaa
     8281 aaggcctgca catcgatgca ttgctcgagg cagtcttgct cgaagccgaa gttttggaac
     8341 tgaccgcacc tgtcgatgca cctgccaaag gtatcatcgt tgaggcgcgc ttggacaaag
     8401 gccgcggcgc ggttgccaca ttgctggttc aaagcggcac gctgaaaaaa ggcgatatgc
     8461 tgctggccgg tacggcattc ggcaaaatcc gcgcgatggt cgatgaaaac ggcaaatcca
     8521 ttaccgaagc cggtccgtcc atccccgtcg aaatcctcgg cttgtccgac gtaccgaatg
     8581 cgggtgaaga cgcgatggta ttggcggacg agaaaaaagc gcgcgaaatc gccctcttcc
     8641 gccaaggcaa ataccgcgac gtgcgccttg ccaaacagca ggcggcgaag ctggaaaata
     8701 tgttcaacaa tatgggcgaa acccaggccc aatccttgtc cgtcatcatc aaagcagacg
     8761 tgcagggctc ttacgaggct ttggcgggca gtctgaaaaa actgtccaca gacgaagtga
     8821 aagtgaacgt gttgcacagc ggcgtgggcg gcattaccga atcggatgtc aaccttgcca
     8881 tcgcgtccgg ggcgttcatt atcggcttta acgtgcgtgc agatgcctct tcgcgcaaac
     8941 ttgccgaaaa tgaaaatgtg gaaatccgct actacaacat catctatgat gccatcgacg
     9001 acgtgaaagc cgccatgagc ggtatgcttt ccccggaaga gaaagaacag gttaccggta
     9061 cggtcgaaat ccgtcaggtc atctccgttt ccaaagtcgg caacattgca ggctgtatgg
     9121 ttaccgatgg tgtggtcaaa cgcgattccc atgtccgcct catccgcaac aacgtggtca
     9181 tccacacggg cgaactggct tcgttgaaac gctataaaga cgacgtaaaa gaagtccgca
     9241 tgggctttga gtgcggtctg atgctcaaag gctacaacga aatcatggaa ggcgaccaac
     9301 tggaatgctt cgacatcgtc gaagttgccc gcagcctgta attcctttgc aaataaaatg
     9361 ccgtctgaag cgttcagacg gcatacgaaa cgggttctgt atcatacaga acccgttttt
     9421 tgtcgcaaat cggcttcaga cagccctctt gccttatccc gatttgaatc tgacttgcca
     9481 tacaaacagg cttcagacgg cattatagtg aattaaattt aaaccggtac agcgttggct
     9541 cgccttgccg tactatttgt actgtctgcg gctcgccgcc ttgtcctgat ttaaatttaa
     9601 ttcactatat tttcccgaca ggcggatttg aagtttctcc acatatccgg cgcgttcggc
     9661 gcggctggtt tcggggcggt gcgtattgag cgacgaccat ttccaatgac tgcgggcttt
     9721 gttgagttcg ggcgggagtc tggcggcgtc ccacggggct ttgcggctgt gcaactcgat
     9781 atccgattgt gccgcgtgtc cgcgcgtttg caggacgtgg agcagatcga gggcgcgggc
     9841 ggcgagcagg gtcagggttt cagggtcggt gtgcaaggtt tggcggccgg cgagtttgtc
     9901 ggaaatggtg cgggtattgg gcaggatgcc gcccaaaaag ccgccgattg ccgtacccaa
     9961 tccgagcgag ccgccgagtg tggcgatgtc cagccccaag ccgatgagtg cgccggttgc
    10021 cgcgcccgtg ccggtgcgga tgccgtattg tttgagcaat tcgctgtcga acgggtcttg
    10081 gcggaaggct tgcggcatcc agtcgccgcc gtcgatttcg ctgtggtaga aacggtagag
    10141 ggcaaacagc cgctgctgca tctgccgttc gagttggcgt atttccgcct gcatggtttg
    10201 tagcacggtg gcggtatcct cgttttcgtc cacttcctgc ctgaaggcgg cggcatcgag
    10261 caggaaatcg gcgatttcgc ggcgcgcttc gccgtccagc cgctgccatt cgcgccggcg
    10321 catggctgtc aggcggtcaa gtgtgctgcg ttcgggcaac atggtggcga ggttttccca
    10381 caggcgtagt tcgccttcaa aatcaaaggc gacggtgtcg acccctgcga aaacgtgcag
    10441 gtttctcctc gccagcatgg ttgtccacga ttcgggaagc tgtccgccgg taaagttgaa
    10501 cacgggcata actggtttgg cacaccatga aaggatggtc aattcatccc gatatttgtc
    10561 gaggacgggc tcgcgcgcgt cgatgacgta cattgccata tcgctttgca agacttgccg
    10621 taagactttg gcttcctgat tgaaatcatg gtgcgcgccg tggctgccga gaaactgttg
    10681 cagccgttcg atgccgtctg aacgattgtc cgtatggttt tccagccatt ccagcacgcc
    10741 gcccgcgtct tcaagtccgg gcgtgtcgta caggaaaacc agcgtgtctg cgccgtcgct
    10801 gatggcggct tcttcgacat ggcgtgtggt cgatggggcg tttctgactt cgccgaaacc
    10861 gctgtcgcgc aacagggtac gaaggagcga ggttttgccg gtattggtat gcccgacgac
    10921 ggcgagggaa aggggttgtt tgttcatgat gtttttgaag aatggatttt cagacggtct
    10981 tttttcagaa tggcggctta acagaacatt tcaagtgagt ttattggtct ttcaaacgcc
    11041 cttcctgcgc cgccctgtca ggctcaagcc acgccgcgcc gcattcggcc agcgcgttac
    11101 gccaatgttc cagcttttcc gaaaggtcgt ctgaaagccc ctgttccgcc aaaagctgca
    11161 ccaccgcgcc gccctgcgcc gcttccgaaa gtcggacgat ctgccgcaac acgccgcggt
    11221 cgggcacagt ttgggcgcgc acgccgataa gcagttgcgc cggtttctgc ttcagctctg
    11281 tctccagcgc ggcaacctgt tcccgattgg cggcaacgcc cttatccagc cattcctgcg
    11341 ccagcctgcc ctcgaaccat tcgccgtcct gccattcggt ctccagcatg accgcccatt
    11401 tcggcgcatc gttcaagacg attttcggcg aaacggcgga cacggtttcc cgacgcgtat
    11461 ccgcatcggt gattttgttc tgccagcggc ggatgaccgc ctgataatag ggtttttcca
    11521 aatccaatcc gttttcgctt gttttcaaaa ggattttgca taccgcccaa gccaagaggc
    11581 gcggcaggat gccgtagcag gcgatactgc cgaccagcag ccccgaccaa gcccgcgcat
    11641 cggcaatatt gccgttcaga cggccttcga tgaccgcccg cgcatcaggc acgggaaaac
    11701 ccagtttcgc aggcagccat gccaacattt ccaccagccg taccgaagac gaatcgccca
    11761 acagcgtgct ttcccagttg aacgtatatt gccgcaccaa aagcagcaac aataccgaca
    11821 ccagcattcc gagcagcgtg cagagccaca ggctgtgcga cgttgcgcct attttccaac
    11881 gtaccgaagg ttgccgccac tcgtccgcat acagccgcaa caccgcctga tttacagggt
    11941 ctttgccccg aaaccacgtc gccggactgc tgaaaaaacg ccccactttc acgcgcagga
    12001 acaacattgc caaccatact gccagcatca gcgtattcat gcccaacacg cccgccaaaa
    12061 ccaaaaagaa attcagaccc tgattgtcca ttagaagata agtgcctgaa aaaccggtaa
    12121 aaaacgcaaa cgtcgccgcc gccacccaca accagaacga ccccgcacgc acacgttcca
    12181 atgtgtcccg cagcatacgg ttcctgtcga tcatctccgc ccgacggatg attttttcct
    12241 ccgtactgcc gtccacgcgg cgcaaagcct ccgtcgcctg cacgggatcg ccgctgaaaa
    12301 taaaaccgcc ttcgtccaaa atacggatca gctcaaccag ttttcgggat ggattcaaca
    12361 taaaatgccg tctgaaaata aaaaacagat tttaacacac gcattttcaa gaatattcac
    12421 agtgtaggca aagagtaaat ctcacacaga agcaaaagta tcggcgtaaa ctgactgcct
    12481 ctactttccc gaaagattgt gcgatgtata caggcgaacg cttcaatact tacagccatt
    12541 tgagcggttt gattctggcg gcggcaggtt tggcgctgat gctgctgaaa accataggac
    12601 acggggacgg ctaccgtatc ttcagcgtat cggtttacgg catcagcctt cttctgctct
    12661 atttgagttc ctcgctgtac cacggaattg cagccggaaa actgaaaagc attttgaaaa
    12721 aaaccgacca ctgcatgatt tatgtgctga ttgccggaag ctacacaccg tttgcactgg
    12781 tttctttgag aaacgggccg ggctggacgg tattttcact gtcctggctg ctggcggctg
    12841 caggaatcgc acaagaactc accatcggac ggaaaagcga aaaacgactg ctgtctattg
    12901 cgatttatgt cgtcatgggt tggatggtct tggcggtaat gaaatccctg acagcctcac
    12961 tcccgctggc aggactggct tggctggcgg caggcggtat gctgtacagc gtcggcattt
    13021 actggtttgt aaacgatgaa aaaatccgac acgggcacgg aatctggcat ctgttcgtat
    13081 tgggcggcag catcacccaa tttgtcagtg tgtacggtta tgtaatctga atgccgtctg
    13141 aaaagcaaaa cctcccgatc ctgaagattg ggaggttttc tgtttgccgg acatcagccc
    13201 ttgtcgtgga actcgtgaaa ttcatactga taggacaaat cccgacccgc ttttttctgt
    13261 gccaaataat catcataaat ggcacggatt tccttacgca acaaaaacag ggctatcaag
    13321 ttcgggataa ccataaagcc gttgaacata tcggacaggc tccaaaccaa atcgactttg
    13381 ccgagcgtac ccaaaacgat ggcaagcaga accaatgcgc gatagatgcc caagtgtctt
    13441 cccctgaaaa gaaaacggat gttggactca ccgaaataat accagccgat aatggtcgtc
    13501 aaggcgaaaa aggtcagaca cacggcaagc aattgcgaac cgaagcccgg aaatgccttg
    13561 ttaaaggcaa actgggtaac cgccgcgccc tgttcgcccg aaaggttggc atcagtcagc
    13621 aggataatca atgccgtagc cgtacatacc aaaatcgtat cgataaacac accgacaaat
    13681 gccgccatac cttgctgcac aggatgcttc acatcagcgg ttgcatgtgc atgcggggta
    13741 gaacccatac ctgcttcgtt ggaaaacaga ccgcgcgcca cgccgaaacg tatcgcttca
    13801 cgcataccga tacccgcagc accgcccaaa acggcttcag gattgaaggc ggcggtaaag
    13861 atgtggttga acatcggcac aatatagtcg gaaaattcaa acaggataac gacggcgcac
    13921 aaaatataaa caaccgccat aaacggcacg acaaactggg caatattggc aatacggttc
    13981 acgccgccaa tcacaaccat gcccgcaagg acggcaagca caataccgac tgccaaagaa
    14041 ggcacatcaa atgcaatggt aacggcagaa gcaatggagt ttgcctgtgt cgcattaccg
    14101 ataaagccca atgcgataat caacgcaatg gagaaaaagc ccgacaggaa acgcgccgcg
    14161 cccctgccga ttttcggagt cagaccgtgc gtaatataaa acgccggccc accgatgtat
    14221 ttgccgtggc tgacgacgcg gtatttctgc gccagcagtg cctccgcaaa aatcgtggac
    14281 atccccaaaa cggcagaaac ccacatccaa aaaatcgcgc ccggcccgcc tgcggtgatg
    14341 gcggtcgcca cgccggcgac gttgcccgta ccgatttgcg cggatatggc aaccgccaac
    14401 gcctgaaact gcgataaaga cttatcgtct ttatcgcctt tggcaaacaa gccgccgaat
    14461 acagatttga atcccgcacc cagcttggta atctgcggcg caccgagata cagcgtaaaa
    14521 aacaggccga tacccaaaag cgcgtaaatc agcaggtagt cccaaaggaa ccgattgact
    14581 gtacccacca gaacagacaa tatattttcc ataaaataaa ccttatctta caattaaaat
    14641 gactgccttc caaaagacat tccaataagg aaacacggcg agcagaccgt atttgccgca
    14701 acagatgcct taaattgtca acaatcgggg agaagctgcg ccatcatacc gtaaaatatc
    14761 gttaattaaa acatttcttt atttttaagc ggaaagcgga ggaaatcgct ttcagacggc
    14821 atcgacaacg gcacggcata aaacaggata ttttgggtac ttgcaactta tgttaaaatg
    14881 ccgaccgtaa aaaatctgac aaaaacagat taattatttg aaataagaaa ggaaatttat
    14941 ggcaggtcat agcaagtggg cgaatatcca gcataaaaaa gcccgtcagg atgccaaacg
    15001 cggcaaaatc ttcacccgtt taatcaaaga aatcaccgtt gcggcgcgta tgggcggcgg
    15061 cgatcccggc gcaaatccgc gcctgcgcct ggctttggaa aaagcagccg aaaacaatat
    15121 gcccaaagac aatgtgcaac gcgccatcga caaaggcacg ggcaacttgg aaggcgtgga
    15181 atacatcgaa ttgcgctacg aaggctacgg catcggcggc gcggctttga tggtggactg
    15241 cctgaccgac aacaaaaccc gcaccgttgc ggacgtacgc cacgcgttta ccaaaaacgg
    15301 cggcaacttg ggtaccgacg gctgcgtggc gttcaacttc gtgcatcagg gctatttggt
    15361 attcgaaccc ggcgttgacg aagacgcgct gatggaagcg gctttggaag ccggtgcgga
    15421 agacgtggtt accaacgacg acggttccat cgaagtcatt accgcgccaa acgattgggc
    15481 gggcgtaaaa tccgctttgg aggcggcagg ttacaaatcc gttgacggcg acgttacgat
    15541 gcgcgcccaa aacgaaaccg aactctccgg cgacgatgcc gtcaaaatgc aaaaactgat
    15601 tgacgcgctg gaagacttgg acgacgtgca agacgtttac acttccgccg tattgaattt
    15661 ggactgatac gcagcacagc agacatacaa taaaatgccg tctgaacctt tcagacggca
    15721 ttgatttatt tagcccttgc ccacgcccac caaaccgtca ggacaaccgc cagaaaatac
    15781 gccgcgctcc aaacaggcaa agcaacccct aacagataat ccggttcggc acaatttccg
    15841 aacccgcgca cgacaggctc gaaccaatca aacaaagacc agcccttcaa gcggaacgtc
    15901 cacggcgcac cgcacgaagg agcggttccc ggcggcagcg actgcaacca caactgatat
    15961 accgcaacag aaatacccgt aacggccgga atgctgataa agacagcacc gaacaaaccg
    16021 cccgcccttc ttcttggtct gcacatcagg acaattgccg tacacaatgc ggttgccaaa
    16081 acgcataacc gctgactgat acacaaaacg caaggctcca tacccaaaac atactgtgcc
    16141 gccaaagaac cggcaaatgc acagaccgaa acggcaaaca gcagccaaac ggcttttcta
    16201 aataacgggg tcattttctc aacacaccaa tcaaaatacc gatatgccga ttttgctgga
    16261 tatatcccga gatggcaagg gacgaatcga caccggcgca ggtgcggacg atttcggttt
    16321 tcccgatata cacaagacct gacaagggac aaaacggcga tttgcccgca caatgcccgc
    16381 aaaaaaatac atccggagga tttgaatttc ggcaaattta gcgaatcaaa agtttatttc
    16441 aatgaaatta tatgattttt ttgaataagc ggattgacag gtttttgaag gaatttgtta
    16501 ccggatagcc atcgggcaag ttttttgcaa aatttgaatc ggtcgggtaa attttcaaaa
    16561 aataattgac agcggataag aaacggcgga taattcccgc cgtcgagttg cttgatgcag
    16621 cttgattttt ctcctctatt tctcctttgt agacttggca cacattcaac tggatgtgtg
    16681 cattttttta tctccgcttt ttttgaaatt taattacttt tattgtttga aattccattc
    16741 tttaagaaat ttaacaagaa aaacgcttgc tttttgaccg gaaaagctgc ataattcgtt
    16801 ccgttagctg gttcgagtag tcagttaata gtttctcctc tatttctcct ttgtagactt
    16861 ggcacacatt ctattgaatg tgtgcatttt tttatctgaa gcaacaagcc tctgtgcgtg
    16921 atgttgttat gtttcattta ggtgtcaaac cgcatacccg gtctgaaata ttcaatccaa
    16981 atccaaaacc ggattttctt tgacctcctc catcacaaca taactcctac tctccgaagc
    17041 ggcaggcagt tgcaatagga tattgcctag catatcccga taggcagaca tatcgggcaa
    17101 acgtacttta atcagatagt cgtattcgcc cgacaccaag tggcattcca taatttgcgg
    17161 aattttcagc acttcttttt tgaaatcttc gaaaatattg cccgatttgg attgcagctt
    17221 cagctcgaca aaaaccaata gcggtttgcc caacagatgg ggattgaggt gggcgtgata
    17281 gccggaaata taatgttccc gctccaaacg gcgcaccctc tctgtaacgg gcgtggtgga
    17341 caagcctacc ttctcggcaa gctccgtcat cgggatgcgg gcattctgtt ggaggatctt
    17401 aagaatgcgg aaatcgattt tatctagttc tttcatttgg attgccttgt atttattatt
    17461 gattttaaca aataga
//
LOCUS       DAWEWP010000030        17010 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWEWP010000030 DAWEWP010000000
VERSION     DAWEWP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17010
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.030"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(117..1286)
                     /locus_tag="WGN53_000722"
     CDS             complement(117..1286)
                     /locus_tag="WGN53_000722"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ0139737.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1469..1579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1632..2528
                     /locus_tag="WGN53_000723"
     CDS             1632..2528
                     /locus_tag="WGN53_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ0139738.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2572..3636)
                     /gene="tsaD"
                     /locus_tag="WGN53_000724"
     CDS             complement(2572..3636)
                     /gene="tsaD"
                     /locus_tag="WGN53_000724"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ0139739.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALAFAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLKT
                     GREAGAFNVRPRWPLSEIVK"
     gene            complement(3752..4939)
                     /gene="ccsB"
                     /locus_tag="WGN53_000725"
     CDS             complement(3752..4939)
                     /gene="ccsB"
                     /locus_tag="WGN53_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ0139740.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4932..6884)
                     /locus_tag="WGN53_000726"
     CDS             complement(4932..6884)
                     /locus_tag="WGN53_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ0139741.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTVSREGGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7161..7784)
                     /locus_tag="WGN53_000727"
     CDS             complement(7161..7784)
                     /locus_tag="WGN53_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ0139742.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7990..8619
                     /locus_tag="WGN53_000728"
     CDS             7990..8619
                     /locus_tag="WGN53_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ0139743.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8740..11136)
                     /locus_tag="WGN53_000729"
     CDS             complement(8740..11136)
                     /locus_tag="WGN53_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ0139744.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVQKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLEAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11288..12403
                     /locus_tag="WGN53_000730"
     CDS             11288..12403
                     /locus_tag="WGN53_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ0139745.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12406..13005
                     /locus_tag="WGN53_000731"
     CDS             12406..13005
                     /locus_tag="WGN53_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ0139746.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKVEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13006..13653
                     /locus_tag="WGN53_000732"
     CDS             13006..13653
                     /locus_tag="WGN53_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ0139747.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13671..14216
                     /locus_tag="WGN53_000733"
     CDS             13671..14216
                     /locus_tag="WGN53_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ0139748.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14235..16520
                     /locus_tag="WGN53_000734"
     CDS             14235..16520
                     /locus_tag="WGN53_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ0139749.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4578 a         4836 c         3873 g         3723 t
ORIGIN      
        1 ttccgtacta tctgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
       61 tatataaaaa tgccgtctga actgttcaga cggcattttt gattttcaaa ccggaattac
      121 agccccgctg ccgccctcaa tgcagcagct ttgtcggtgc gctcccaagt gaactcaggt
      181 tcttcgcggc cgaaatgtcc gtaagcggcg gatttactgt aaatcgggcg caagagatcg
      241 agcatttgga cgatgccttt ggggcgcagg tcgaaatgtt cgcgaactaa ggtaatcagt
      301 ttttcctcgc tgattttgcc tgtgccgaaa gtgtcgatgg aaatcgaagt cggttcggca
      361 acgccgatgg cgtaggaaac ttggatttgg cattgggttg ccaaaccggc ggcgacgatg
      421 tttttcgcaa cgtagcggca ggcgtaagcg gcggaacgat ccactttgga cgggtctttg
      481 ccggagaacg cgccgccgcc gtgcggagcc gcgccgccgt aggtatcaac gatgatttta
      541 cggccggtca aaccgcagtc gccttgcggg ccgccgatga cgaagcggcc ggtcgggttg
      601 atcaggtatt tggtttcgtc ggtcagcagt tcagacggca ggacgggctt gataatctgc
      661 tcaatgacgg cgtttttcaa ctcttcataa ccgatttccg ggtcgtgttg cgtagagagg
      721 acgacggtat caatgcgttt tactttgccg gattcgctat cgtaaaccac ggtcagttgg
      781 gcttttgcgt cgggacgcag ccaaggcagg aggccgtctt tgcgtaattc gctttgacgc
      841 tgcatcaggc ggtggctgta atagatggca aacggcatca gggtcggggt ttcgtcgcag
      901 gcatagccga acatcaaacc ttggtcgcca gcgccttggt tcaagtcaat gccttcgcct
      961 tcgttcacgc cttgggcgat gtccggagat tgctggtcgt agtacacgcc gaccgcgcag
     1021 ccgttggcat caaagcccag ctcggaggag ttgtagccga tgcgtttgat ggtttcgcgt
     1081 gcgactttga tgtagtccac ttgggcggtg gtggtgattt cgcctgccaa tacgcacaag
     1141 cctgtgttga ccaaagtttc tgcggcgaca cgcgctttgg ggtcttgcgc caagatggca
     1201 tccaaaatcg cgtcggatac ttggtcggca actttgtccg gatggccttc ggataccgat
     1261 tcggaagtaa acagatattc gctcattgag attttccttg aaaaacaaac catcttcttc
     1321 agacggcatg ttgtatgaac ataatgtcga cagcgggaaa tatagcaaaa tttccctatt
     1381 cataccattc agttgagaaa tattcccatt tgaatggcac tttggaatct ctgcccgtac
     1441 gtttcttaca ggcaaaaaaa ttcccgcatc aagcgggttt ggattgctct ggtgagccac
     1501 atcggctttt caaccgtcca ccttactttt ccttttgaaa agcaggttgg catggaattc
     1561 ccaactctta atgcagcgcg gatggtagca gaaaagcctt tccgccgcaa tacttccctt
     1621 tttcagacgg catgtttcgt ttacaattcg ggctgtttcc ccctttgcga accgccatgc
     1681 acatcctgtt gaccgccctg ctcaaatgcc tctccctgct gccactttcc tgtctgcaca
     1741 cgctgggaaa ccggctcgga catctggcgt tttacctttt aaaggaagac cgcgcgcgca
     1801 tcgtcgccaa tatgcgtcag gcaggcatga atcccgaccc caaaacagtc aaagccgttt
     1861 ttgcggaaac ggcaaaaggc ggtttggaac ttgcccccgc gtttttcaga aaaccggaag
     1921 acatagaaac aatgttcaaa gcggtacacg gctgggaaca tgtgcagcag gctttggaca
     1981 aacacgaagg gctgctattc atcacgccgc acatcggcag ctacgatttg ggcggacgct
     2041 acatcagcca gcagcttccg ttcccgctga ccgccatgta caaaccgccg aaaatcaaag
     2101 cgatagacaa aatcatgcag gcgggcaggg ttcgcggcaa aggaaaaacc gcgcctacca
     2161 gcatacaagg ggtcaaacaa atcatcaaag ccctgcgttc gggcgaagca accatcgtcc
     2221 tgcccgacca cgtcccctcc cctcaagaag gcggggaagg cgtatgggtg gatttcttcg
     2281 gcaaacctgc ctataccatg acgctggcgg caaaattggc acacgtcaaa ggcgtgaaaa
     2341 ccctgttttt ctgctgcgaa cgcctgcctg gcggacaagg tttcgatttg cacatccgcc
     2401 ccgtccaagg ggaattgaac ggcgacaaag cccacgatgc cgccgtgttc aaccgcaatg
     2461 ccgaatattg gatacgccgt tttccgacgc agtatctgtt tatgtacaac cgctacaaaa
     2521 tgccgtaacg aaaataaaaa tgccgtctga acaatttcag acggcatttt gtcatttgac
     2581 gatttccgac agcggccagc gcgggcgtac attaaatgca ccggcttccc tacccgtttt
     2641 caaatgcatc gcgcctgcaa aggcaatcat ggcaccgttg tccgtgcagt atgccgtcgg
     2701 cgggaaaaac acgctgactt tttcggacgg atgtttcggc ttgcctttgg gggtcgggat
     2761 ttgcaccgtc atgttgccga aagtttcacg gagtttgcgg tttgcaccga ccccgccggc
     2821 gaccactacg gttctgaacc ctgtctgcaa cagggctttt ttcactttcg ccgccaacac
     2881 atcgactacc gcatcttgaa acgcacggca gatgtcgttg cgtgtctgct caggaatgtc
     2941 atccgccccg ttttccgcgc gcactttctc gacggcagtc aaaacagcgg ttttcaaacc
     3001 tgaaaaactc atctgcaaat cgtcggaatg aatcatcggg cgcggaaaaa caaacgcttc
     3061 gggcctgccc gattccgcaa gttccgacag tttcgcaccg cccggatacg gcaagcccag
     3121 cagtttcgcc gttttgtcga atgcctcgcc cgccgcatca tcgacgctct cgcccaaaag
     3181 cgcgtagtcg cctatgcccc tgaccgccat aatctgcgta tgcccgcccg aaaccaacag
     3241 cgcgacaaaa ggaaagtcgg gtttttcctc cgccaacagc ggcgacagca gatgcccttc
     3301 caaatgatgg acgggaataa caggcttatc cagcgcaaaa gccaaggcat tggcgtaact
     3361 cgaacccgcc agcaacgcgc cgcccaaacc gggcccctgc gtaaaggcaa ccgcgtcaat
     3421 gtcgccatac gatgcgcctg cctgcgccag acagccttcc gtcaacggaa caaggcggcg
     3481 gatatggtcg cggcttgcca gctccggcac aaccccgccg tattcggcgt gcattgccat
     3541 ctgcgtgtgc agacagtgcg cccgcaatcc tcgttcggtg tcatacagcg caacgcctgt
     3601 ttcgtcgcaa gaagattcga ttcccaatac caacatggtc tgatgccgtt aaaaactgaa
     3661 aaacgtattc tagcggattt cggcacgact gccgtatccc aaaaacggaa catgccgtct
     3721 gaagcccttc agacggcatc gtcgcaccgt atcaaagcgt tccgtaagaa tgcagcccgc
     3781 tcaaaaacat attcacgccg ataaaggcaa atgcggttac gaacaaaccg ataatcgccc
     3841 accacgccag cactttgccg cgccaaccgg caaccagccg caagtgcagc caaacggcgt
     3901 aattgagcca gacgatgaac gcccacgtct ctttcgggtc ccaactccaa tagcgtcccc
     3961 aagcatccgc cgcccacagc gcacccaaaa tggtggcaat ggtaaagaac agaaagccga
     4021 cggcaatcgc cttatacata acttcctcaa tcaacacgga ttgcggcagc cacgatttcc
     4081 cgcctttttc ttccgcacgc agggaaacca gttcggcaat accgagcatc gcggaaatgc
     4141 aaaacgcgcc gtaaccgata aagtttgccg gaacgtggat tttcatccac caagattgca
     4201 atgccggaat cagcggctgg atggtatgcg cctcgcggga cacgctgtac cacaagacaa
     4261 atccaaccac gaccgccata aagccgaaca cgaagccgcc caatttctgt atggcgaact
     4321 taccttcata ataaagatac atcagcgcgg taatcaccaa aaacaggatg aacacttcat
     4381 acaggttgga aaccggaata tgccccgcat cgggacggag cagatagctt tcgtgccaac
     4441 gtaccagcag accggtaaag cctgctacgg cagacaccca tgcaaacacg gttcccatac
     4501 ccaacagcgt gttggtcggc acatttttta cgcttgccaa aaccgcgccc gaaatatagg
     4561 cgaacagggc gaagaagaca aaggcgcact gccacataat cgccgactgg ctgctgagga
     4621 aataccgcaa caggaaaatc tctgccgatt tgatgttgcc gccatacaaa ccgacggcgg
     4681 cataggcaag caatacgctt aaaggaacaa accagcgcat cggtttgaaa aaccaaccca
     4741 aaaacacggc aataccggca cttgcccaca acatgaccgt ttcgtaaatg tccatatgca
     4801 taccggaacg ggtctgtacg aaaaccgtag ccgcaaaaac cagcacggca aatacccaat
     4861 cccaaagatt cagattgctg atcaaagact tctgaatcag cagctcgtgt tccggaaggg
     4921 ttttatagtg ttcagtcatg attcaagtcc ttgccgagcc gttgcagact ctcgacgtgt
     4981 tttggaaatt ccttctgcaa atcccgttcg ctgcgggccg aagacatggc aaaacggatt
     5041 ttgccgtctg aaaacaatac ccacgcccgt ttttcgcgca cataaaacat caataccgta
     5101 cccaatacca acagcaccga gccgagatag accaaaagcg cacccgggga acgggtcatc
     5161 tgcaaacccg acgaacgcac ctcggaaaac ccatcaagtt gcagcagcat aggcgcggga
     5221 tattcggtca aacccgtgta cgcatccata ctgtgcagca ggaaacggtt ccgcgcttca
     5281 tcctgctgcc attcgggcaa gccgtaccgg cgtatggttt catccaaagc agcgttcatc
     5341 acgccgtaaa gcatttcgta gaaataaccc tgcatcttat cctgctgctc tttcgggata
     5401 ttggacgtaa taaattcgtc caatcccaaa tagccttttt gtgcaaagat gttcagcgtg
     5461 ttttccgcag ccagcatgaa ttgttcgcgg atttcggcag gtgcgtcttt ggttgcgtcg
     5521 gcaaccagac gtttgcgccc ttccccatct ttcaaaaact cacgcaatgc cataaaggtg
     5581 tccgctttca actgcttgtc caaggggata cgcagccagc ggtattgctg ctgcaagccg
     5641 ctgcgcgtgc cggtaatcca aaaataatcc tgttcctgca aaaccggcag catatagttt
     5701 ttatattcga ccgcctgccc tgccgcatca cggatacggt aaacaatgga agggccgata
     5761 ttggtgtatt ttttaccttc ctgagtaacg gcgcggacat cgttcagcgt ggatttcagg
     5821 cttttttccc gttccgcgcc ctcgctcatg tcctccacat tcatagaagt gaactgatcg
     5881 aactcaagac gatatttgtg tttgccaatt tccaacggaa actggtgtat ggatgttgcc
     5941 ttcaacacga caggctcgcg cgaagcatca cccaaattcc acgccttgaa tgtcaaatcc
     6001 gaaccgccgt cggcaaaact cgcctgataa atcgtgatgc cgtgcaaggt caaaggatgg
     6061 ttcacgcgga tggtgcgctc gagtttctca ccggttgcct tgtccgtcac ttcaatatcg
     6121 ctggcgaaat cacgcggcat acccgtattg taaaaatcga tatggaattt tttcagtttg
     6181 acttcaaaag gcaagtcctg aaccaatatc ccgttgtcgg cattcaggaa aaccacatcc
     6241 gcactctgcc cctcggaaat attgacgttg cccctaaatg agagattgga cgcactcaaa
     6301 atactttcgg gcttgaaatc cttggcataa accgcctgat tgtccggaac aatccgaccg
     6361 gtcagcatac ccagtttcaa cagcaggtta ctgtctatca acccgcccag gcaaatgaca
     6421 atcaaagcaa catgggcaaa gatatagccc catttgttca tcgtgccttt tttggcggca
     6481 atcagaaccg acccgccctc acggctgacg gtttttccct gaaaaccctg cacctccaga
     6541 taacgtttgg caacttcggg ggcaattttt acatccaaca gcgaagaatg gcgcatcgcc
     6601 gccagagatt tttctttaac cttttcccga aaagacttca tttcgcgcca gaacggcggc
     6661 acattgcgaa tcaggcacaa actggtagaa accaccaaaa acatcatgat aacgacaaac
     6721 catgccgaag catagacgtc atacagtccc agaaaaccaa aaatctgcgc ccaaaaaggt
     6781 ccgaatttga ccaaataatc cgtctgcggc tggttttgct gcaacaccgt accgataacc
     6841 gatgcaatac ccagcagact gagcaaagcg accgcaaagc gcatggagct gaaaaaagcg
     6901 aaccacggac gggaaagaag tgggggagat ctacgggatt tactcattgt gtgtttattc
     6961 cgccatcagg aatatgggaa agcagaattg ggcaaacaga aaacaacgtc ccgattctac
     7021 tgtcttgatg ctttttattt caagacaatg aagacagcct gcatcgattc caacggttgc
     7081 gattgaaaaa acttatcgca gaattgcctg aagccgtctg aaaacttttc agacggcctc
     7141 taaaacagac tattgcggaa ttaacgcaaa ccttggataa agttggcgac cgctttcaaa
     7201 tcttcttcag acatacggtt tgcaatatct tccatgatgg tatttttacg ctgaccggac
     7261 ttgtaggcat tcatctgttc aacaatatat gcctgatgct gaccgcccaa acgcggataa
     7321 gcctgaattt cgcttccgcc tcccggcata cccgcaccgc tcggaccgtg gcaggacata
     7381 cacgccggca cttttttatc gctcaaaccg ccgcgataga ttttcgcacc caattcggga
     7441 ttttccttag gattggcttc accggatttg ggctgctgtt tggcatagaa tgcggatacg
     7501 ttcaaaatat cctgatcgct caaattcatt accaccggtt tcatcacagc tgccgaaccg
     7561 tgggtgcgtt taccgtcgcg gatgccgata gtttgatgat agatgtaagc agtatgctgt
     7621 gccgccaaac gcggatacat cgcaatgccg ctgttaccgt ctgctgcatg gcaagccgca
     7681 caaaccgttg cggcaacctg tttgcctttt tccacgtctg ctttgggaga ggcggaaacc
     7741 gcaccggcag ccaaaacaaa ggccaataaa gtcaatcgtt tcatggagtg ctcctgatta
     7801 cagcattgga taacgcaaca atgctctttt tatattcaaa tacgggattt ttgacccgat
     7861 taaaaccgat gattctgtaa acgtgttatt ctatactaaa tttacattaa attaccactg
     7921 tgtttcacat aaaaccaacc gcatattttt gctgtcggac aaacggcggc ggaaaacaag
     7981 gatatgccca tgaacctttt tcaaaacgcc aaattcttca cgacggtcaa ccaccttaaa
     8041 gacctgcccg acacccctct cgaaattgcc tttgtcggca ggagcaatgc cggaaaatcc
     8101 agtgccatca ataccctgac caaccatgtc cgtcttgcct acgtttcaaa aacacccgga
     8161 cggacgcagc atatcaactt cttcgagctg cagaacggca attttatggt cgatttgccc
     8221 ggctacggtt atgcccaagt ccccgaagca gtacgcgcac attgggtcaa tctgctcggc
     8281 gactatctgc aacagcgcaa acagcttatc gggctggttt tgattatgga tgcccgccat
     8341 cctttaaaag aactcgacat ccgtatgctg gactttttcc acacgaccgg cagaccggtt
     8401 cacatcctgc tgtcaaaagc cgacaaatta tccaaaaacg aacagataaa aaccctgtcc
     8461 caagtcaaaa aactgctcaa accttattcc gacaggcaaa acatcagcgt acagctgttt
     8521 tccagcctga aaaaacaagg tattgacgag gccaaccgaa ctgtcggaag ctggtttgac
     8581 gcagcagatg ccgccgcttc ctctccaaag gaaaactgac cccaattata cggaaaccgt
     8641 atttccccca cttgaccgac cgcaaacatt taaaaaattg ccactgccaa atctaaaatg
     8701 ccgtctgaaa gacttttcag acggcatttt gcggagtctt taaaacagag aatccaactg
     8761 ctgctgtttg gaaccagtat tactcggaag caccggcgtt tcctgcatat cttggcggac
     8821 ttcgtcatcc gccgcctgcc gtccgccttc tgccgcgccc ccgtcatctt cttttgcccg
     8881 tcgggaaggt tgcggcgcaa taccgctgtt gtccagcgtc aagcccggat cggttaccat
     8941 acgttccttc atatagtatt cgccattgct gctgaccaca ccttcaggca ttttcatccc
     9001 cttgccctgc tttcctttca acgcaaaacg catatagtcc acccaaaccg gcaccgcaat
     9061 cgtaccgccg tagccgacac gccccatact cttaggtttg tcgaagccga tatatacggc
     9121 agtaaccaca tcagggttaa aaccgacaaa ccacgcatcc ttattgtcat tggtcgtacc
     9181 cgttttaccg gcaatatccg ttcttcccaa cgcagctgcc ccccttgccg taccgacacg
     9241 gaccacatcc tgcataatct tatacataat ataggcattg cgcggatcga ttgcctgagg
     9301 cgcattttgc ccggcctcca aaggttgcat ttgggcgcgc aacctgccgt ctctgtcata
     9361 aatcttatcg attacgtgcg aagaaaccct atatccgccg ttcgcaaata cgctatatgc
     9421 ctccgccact ttcaacggcg ttgtctcgcc cgtacctaaa gccatagaca ggcttgccgg
     9481 cagctcggac gacctgaagc cgaaacgccg gatatactgt tgcgcgtaac cgacaccgat
     9541 agacatcaaa atacggatgg aaaccatatt cttggaagcc gtcagagcct gtctcaaagt
     9601 aatgtagccg gaatatctgc cgtctgaatt tttaggtgtc caaaccgaac cgttcggccc
     9661 tttccccggc agggaaatcg gcgcatcgtt aaccactgtg gacgcggtca tccccttaga
     9721 taatgccgcc gaatagacaa acggcttaaa ggtcgaaccc ggctgccgca ttgcctgaac
     9781 ggcacgattg aatgttttgc tgtgaaaatc ataaccgccg accagcgcgc gcacagctcc
     9841 ggtttttgca tccagcgaaa ccaaagcccc ctgcagcaac ggctcttgaa ccaccgccca
     9901 acgcccgccg ttgtttttga cacggatgac cgcgcccctg cggatacggt cctcccccat
     9961 tttttcatta ttgaccgcgc gggccgcaaa acccaaggcg cgcctgtcaa gcgtaacccg
    10021 cctgccgccg ggcagctgta tgacgacatt tttcttttta gtcacatcca acacaacggc
    10081 gggaaccatt ttatcgacgg tatagagtcc cgacagatac tggctgacag tctcctcgac
    10141 atcttcactc ttactcaaat cgatatagtt ttccgcaccg cggtagctgc tgccgcgatc
    10201 gaaattccgt agagccttgc gcaatgcctc ggttgccacc ttctgatgat cagcgcggac
    10261 cgtggtataa accttaaaac cctgcgtata ggcatcttca ccgtatttct catacagttc
    10321 ctgacgcacc atttccgcca catataaagc actctgatcg attttctgaa caaaccgctc
    10381 gtaatgcagt tcctcattca acgcctgatc gcgctgttgc acggtaatca tcttctcctc
    10441 gagcatattg ttcaaaatat acttctggcg caacttggca cgttccggat taacaatcgg
    10501 attataggca gacggagcct tgggcagtcc cgcaagcatg gcggcttccg ccaaagtcaa
    10561 atctcggaca ttcttattga aatagatttg cgcggcagat gcaaaaccat aggcgcgctg
    10621 accgaggtaa atctgattga aatacaactc gaggattttg tctttgctta aagactgctc
    10681 gattttatag gcaagcaaca cctcattgaa tttgcgtgtg aacgtttttt cactgctcaa
    10741 ataaaaattt ttcgccacct gctgcgtaat cgtactcgca cccgactgca cgctgccgga
    10801 cacgacattg ccgacggcag cgcgggcaac accccaaaca tccacccccc aatgccggta
    10861 aaagcgttta tcctcggcgg cgataaccgc attccgcaac acctctggga aatcgccgat
    10921 ttttgtaaat tcgcgccgct gctccccata cataccgatg acttccccat ccgccgaata
    10981 aatagtcaac ggcattttag gctggtaatg ctgcaaagaa tccaaagacg gcagtttcgg
    11041 atacgttacc aaaatagcaa tggcaaccaa acccactcca aatacacaaa acccaaaaac
    11101 caaaccaaaa caagtcgtta aaatcttttt aatcatagct gaataataat ttaccattat
    11161 tggtattaaa taaagtaaaa tagcaaccga tttctacaaa gcacggtttc aatgtgcaaa
    11221 gaacaaggaa tccattacgg ataccgaaac ggttactcac tgtacaaata aagcaggaac
    11281 tttcatcatg cgcttgttta aaagcttgaa aaaccctaaa aaaacagatg ccaagctccc
    11341 taaaaaatct tcgggactca ataaccgcgc ggcaatcggc atcgatatcg accagcattc
    11401 catcaaaatg gtccaattgt caggacgtag tttaaaccaa attcaattgg aaaaatacgt
    11461 cattgccaaa ttaccaaaga atatcattca aggcaataaa gtccaaaatt acgatcaact
    11521 tgttacatat ttgcaacaag cctatgccaa actgggtacg tcgtgcaaaa acatcatcgc
    11581 gtccgtcccg caaaatctgg caaccatcga acaattgacc tacacagaca aagatgcaga
    11641 attagacctg caggggttcg tggagtcctc catctccgaa gtcagctcga tatcgctcga
    11701 agaagccaat tacgactatc aggtcttgtc ccaatcggcc gccggcgaag ctgtgttggc
    11761 cgtcgcatcg agaaaggatg aaatcgaacc cctgattgac gcattcaacg ccgccggtat
    11821 gaaattatcc gcgcttgatg tggacatttt cggacaatac aacgcctacg cgctatggat
    11881 aaaccatttc gcccccgagc ttgcagccga aaaagtcgcc attttcggcg tatatgccgc
    11941 acagacctac gccttggtca tccaagacgg aaaaatccta tacaaacagg aaacctccgt
    12001 cagcgaagaa cagctcaacc aactcatcca gcgcacctat caggtaacag aagaaaaagc
    12061 ggaagaaatc atcaactccc cgcaaaaacc ttccgattac caagaaagcg tggcaaacta
    12121 tttcaaccag cagattaccc aagaaataca aagggtcttg cagttttatt acaccacgca
    12181 gaccgcagac gatatgaccg acatcaagca tatcctgctg accggggaag cggcgcgcca
    12241 ggaaggcatc gcccaaaccg tcgcctcaca aaccaatgca gatgtacaat gcgtccatcc
    12301 cgcgcgttat tttgcggaca acctcaaaac agacaaacaa caattcgaac ttgatgcgcc
    12361 gacactgacc agggcgttcg gtttggcggt acggggatta taattatgaa caatttaatc
    12421 aaaatcaacc tcctccccta cagggaagag atgaacaagc gcaaacagca gcagtttaaa
    12481 acgctgatgt acggtgccgt gctgacgggc gttgccgccg ttgcggcaac ctacctgttt
    12541 atcgacaata tgatcaataa ccagtcggaa agaaacacgc tgctggaaac ctccatcgca
    12601 cacttggata ccgagctgtc ggaaatacaa aagctcaaac aggaaaaaga tgccttcctg
    12661 attaagaaaa acaaagtcga ggagctccag ctcaaacgcc tccaagccgc aaaaatcctc
    12721 gacagcctga atgaggccgt ccccggaagc acctacctga cctcgctgga tgccgttacc
    12781 gccgactctt atcggctcag cggcaggaca tccagcgaca accgcgttgc cgccatgatg
    12841 agggcgatgc ccaataccgg catattcaag caacccgaat tgttaagcat caagaaaaac
    12901 aattcgcatc aagaatttac ccttcaggca acattacaac ccatcgtaaa ggcggccgaa
    12961 tccaaagaga atccggcttc gggaaacgca caggaggcaa actgaatggc ttctaaatca
    13021 tctaaaacca acttggatct caacaacctt cacctgctca accttcctgc caggcttttt
    13081 atcgccctgc tggccgttgc cgccgtgctg gggctcggtt atgccggatt gttcaaaagc
    13141 cagatggaat cccttgagga atacgaagca aaagaaaccg aactgaaaaa cacctacaaa
    13201 cagaaaagta tcgacgcggc cagcctgaac aacctgaggg acgaacttgc ctcaatccgc
    13261 tctgccttcg atatcatgtt gaaacagctg ccgacagatg cagaaattcc caatctggtt
    13321 caagagcttc atcaggcagg ttcgagcaac ggcctgcgct tggacagcgt tatgccccaa
    13381 cctcccgtag atgacggccc catcaaaaga ttaccctatt ccatttccat taccggaaat
    13441 tacgaacaga tcagccaatt tacccgcgat gtcggcagcc tctcccgaat cattaccctt
    13501 gagtcgctga aaatcgccca atctccggaa aacggcggca atcctgacgg caagagcagc
    13561 atcctgaacc tcagtgccat tgccaccacc taccaagcaa aatccgtaga agagcttgcc
    13621 gcagaagcgg cacaaaatgc cgagcaaaaa taacttacgt tagggaaacc atgaaacact
    13681 atgccttact catcagcttt ctggctctct ccgcgtgttc ccaaggttct gaggacctaa
    13741 acgaatggat ggcacaaacg cgacgcgaag ccaaagcaga aatcatacct ttccaagcac
    13801 ctaccctgcc ggttgcgccg gtatacagcc cgccgcagct tacagggccg aacgcattcg
    13861 acttccgccg catggaaacc gacaaaaaag gggaaaatgc ccccgacacc aagcgtatta
    13921 aagaaacgct ggaaaaattc agtttggaaa atatgcgtta tgtcggcatt ttgaagtctg
    13981 gacagaaagt ctccggcttc atcgaggctg aaggttatgt ctacactgtc ggtgtcggca
    14041 actatttggg acaaaactac ggtagaatcg aaagcattac cgacgacagc atcgtcctga
    14101 acgagctgat agaagacagc acgggcaact gggtttcccg taaagcagaa ctgctgttga
    14161 attcttccga caaaaacacc gaacaagcgg cagcacctgc cgcagaacaa aattaagaag
    14221 aggattactc cattatgaat accaaactga caaaaatcat ttccggtctc tttgtcgcaa
    14281 ccgccgcctt tcagacagca tcggcaggaa acattacaga catcaaagtt tcctccctgc
    14341 ccaacaaaca gaaaatcgtc aaagtcagct ttgacaaaga gattgtcaac ccgaccggct
    14401 tcgtaacctc ctcaccggcc cgcatcgcct tggactttga acaaaccggc atttccatgg
    14461 atcaacaggt actcgaatat gccgatcctc tgttgagcaa aatcagtgcc gcacaaaaca
    14521 gcagccgtgc gcgtctggtt ctgaatctga acaaaccggg ccaatacaat accgaagtac
    14581 gcgggaacaa agtttggata ttcattaacg aatcggacga taccgtgtcc gcccccgcac
    14641 gccccgccgt aaaagccgcg cctgccgcac cggcaaaaca acaggctgcc gcaccgtcta
    14701 ccaagtccgc agtatccgta tccaaaccct ttaccccggc aaaacaacag gctgccgcac
    14761 cgtttaccga gtccgtagta tccgtatccg caccgttcag cccggcaaaa caacaggcgg
    14821 cggcatcagc aaaacaacag acggcagcac cagcaaaaca acaggcggca gcaccagcaa
    14881 aacaaaccaa tatcgatttc cgcaaagacg gcaaaaatgc cggcattatc gaattggctg
    14941 cattgggctt tgccgggcag cccgacatca gccaacagca cgaccacatc atcgttacgc
    15001 tgaaaaacca taccctgccg accacgctcc aacgcagttt ggatgtggca gactttaaaa
    15061 caccggttca aaaggttacg ctgaaacgcc tcaataacga cacccagctg attatcacaa
    15121 cagccggcaa ctgggaactc gtcaacaaat ccgccgcgcc cggatacttt accttccaag
    15181 tcctgccgaa aaaacaaaac ctcgagtcag gcggcgtgaa caatgcgccc aaaaccttca
    15241 caggccggaa aatctccctt gacttccaag atgtcgaaat ccgcaccatc ctgcagattt
    15301 tggcaaaaga atccgggatg aacattgttg ccagcgactc cgtcaacggc aaaatgaccc
    15361 tctccctcaa agacgtacct tgggatcagg ctttggattt ggttatgcag gcacgcaacc
    15421 tcgatatgcg ccaacaaggg aacatcgtca acatcgcgcc ccgcgacgag ctgcttgcca
    15481 aagacaaagc cttcttacag gcggaaaaag acattgccga tctgggcgcg ctgtattcac
    15541 aaaacttcca attgaaatac aaaaatgtgg aagaattccg cagcatcctg cgtttggaca
    15601 atgccgacac aaccggaaac cgcaatacgc ttgtcagcgg caggggcagc gtgctgatcg
    15661 atcccgccac caataccctg attgttaccg atacccgcag cgtcatcgaa aaattccgca
    15721 aactgattga cgaattggac gtacccgcgc aacaagtgat gattgaggcg cgtatcgtcg
    15781 aagcggcaga cggcttctcg cgcgatttgg gcgttaaatt cggcgcgaca ggcaagaaaa
    15841 agctgaaaaa tgatacaagc gcattcggct ggggggtaaa ctccggcttc ggcggcgacg
    15901 ataaatgggg ggccgaaacc aaaatcaacc tgccgattac cgctgccgca aacagcattt
    15961 cgctggtgcg cgcgatttcc tccggtgcct tgaatttgga attgtccgca tccgaatcgc
    16021 tttcaaaaac caaaacgctt gccaatccgc gcgtgctgac ccaaaaccgc aaagaggcca
    16081 aaatcgaatc cggttacgaa attcctttca ccgtaacctc aatcgcgaac ggcggcagca
    16141 gcacgaacac ggaactcaaa aaagccgtct tggggctgac cgttacgccg aacatcacgc
    16201 ccgacggcca aatcattatg accgtcaaaa tcaacaagga ctcgcctgcg caatgtgcct
    16261 ccggtaatca gacgatcctg tgtatttcga ccaaaaacct gaatacgcag gctatggttg
    16321 aaaacggcgg cacattgatt gtcggcggta tttatgaaga agacaacggc aatacgctga
    16381 ccaaagtccc cctgttgggc gacatccccg ttatcggcaa cctctttaaa acacgcggga
    16441 aaaaaaccga ccgccgcgaa ctgctgattt tcattacccc gaggattatg ggtacggccg
    16501 gcaacagcct gcgctattga tgcgtcaaaa taagggcata tgttttacgg catatgccct
    16561 ttctttatgc tttttgccgc gaccgaaatg ccgtcattcc cgcgcaggcg ggaatccagt
    16621 ccgttcagtt tcggtcattt ctgataaatt cctgttgctt ttcatttctg gattcccact
    16681 tttgtgggaa tgacggcgga aggggtaaat cctcgcaatc caaagcctcg tcatttccac
    16741 aaaaaacagc aacccggaac agcaacttaa aaccccgtca ttcccgcgca ggcaggaatc
    16801 caacttgtcc ggttccggtt gtttttcgtc tcgtaacttt tgagccgtca ttcccgcgca
    16861 ggcgggaatc taggtctgtc ggttcaggaa cttatcggat aaaacggttt ctccaaccct
    16921 gcgttctaga ttcccacttt cgtgggaatg acgggatatg ggtttccgtg cggacgtgtt
    16981 cggatttccg cctgcgcggg aatgacggcg
//
LOCUS       DAWEWP010000031        17007 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWEWP010000031 DAWEWP010000000
VERSION     DAWEWP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.031"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(65..1312)
                     /gene="fabF"
                     /locus_tag="WGN53_000735"
     CDS             complement(65..1312)
                     /gene="fabF"
                     /locus_tag="WGN53_000735"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ0139750.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="WGN53_000736"
     CDS             complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="WGN53_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ0139751.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1936..2943
                     /locus_tag="WGN53_000737"
     CDS             1936..2943
                     /locus_tag="WGN53_000737"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ0139752.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     Q"
     gene            2959..3138
                     /locus_tag="WGN53_000738"
     CDS             2959..3138
                     /locus_tag="WGN53_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139753.1"
                     /translation="MPSERTFCRSDGIFISFSCLPPFENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            3277..3600
                     /locus_tag="WGN53_000739"
     CDS             3277..3600
                     /locus_tag="WGN53_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139754.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            3687..3968
                     /locus_tag="WGN53_000740"
     CDS             3687..3968
                     /locus_tag="WGN53_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139755.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            4021..4494
                     /locus_tag="WGN53_000741"
     CDS             4021..4494
                     /locus_tag="WGN53_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ0139756.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            4748..5083
                     /locus_tag="WGN53_000742"
     CDS             4748..5083
                     /locus_tag="WGN53_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139757.1"
                     /translation="MTVDKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLLSKQGDPDFQGFAEDALDEI
                     SLFIFKKRP"
     gene            5263..7953
                     /gene="glnE"
                     /locus_tag="WGN53_000743"
     CDS             5263..7953
                     /gene="glnE"
                     /locus_tag="WGN53_000743"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ0139758.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRDTKSPQHLSVVAMGKAGGYELNVSSDIDLIFIYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVLTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVGKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLEKLAESGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQRQRLAESMGFDDYADFLNAL
                     NQHRNQVNRLFNEILSEPGEQTQSSGEWQWVWQEKPDEEGRQCRLKEHGFDAETIAAR
                     LDQIRNGHKYRRLSAHAQPRFDALVPLLVQAAAAQTNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWDKLAAELS
                     DGIKACGGDTEAQMDTLRHFQHAQVFRLTVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAIVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALSGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLANYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAEKTEVSGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(8253..8945)
                     /locus_tag="WGN53_000744"
     CDS             complement(8253..8945)
                     /locus_tag="WGN53_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0139759.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(9053..10246)
                     /locus_tag="WGN53_000745"
     CDS             complement(9053..10246)
                     /locus_tag="WGN53_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0139760.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(10375..11112)
                     /gene="pxpA"
                     /locus_tag="WGN53_000746"
     CDS             complement(10375..11112)
                     /gene="pxpA"
                     /locus_tag="WGN53_000746"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ0139761.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(11099..12028)
                     /locus_tag="WGN53_000747"
     CDS             complement(11099..12028)
                     /locus_tag="WGN53_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ0139762.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(12025..12672)
                     /gene="pxpB"
                     /locus_tag="WGN53_000748"
     CDS             complement(12025..12672)
                     /gene="pxpB"
                     /locus_tag="WGN53_000748"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ0139763.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            12856..15063
                     /gene="uvrD"
                     /locus_tag="WGN53_000749"
     CDS             12856..15063
                     /gene="uvrD"
                     /locus_tag="WGN53_000749"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ0139764.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FILDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKIAAFVRLIEALRNQVGQLPLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSIKKTAYDSYGNTRQTATFKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            15298..15837
                     /locus_tag="WGN53_000750"
     CDS             15298..15837
                     /locus_tag="WGN53_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0139765.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(16027..16242)
                     /locus_tag="WGN53_000751"
     CDS             complement(16027..16242)
                     /locus_tag="WGN53_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139766.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(16250..16453)
                     /locus_tag="WGN53_000752"
     CDS             complement(16250..16453)
                     /locus_tag="WGN53_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139767.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(16366..16725)
                     /locus_tag="WGN53_000753"
     CDS             complement(16366..16725)
                     /locus_tag="WGN53_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0139768.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            16778..>17007
                     /locus_tag="WGN53_000754"
     CDS             16778..>17007
                     /locus_tag="WGN53_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0139769.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNR"
BASE COUNT         4250 a         4509 c         4348 g         3900 t
ORIGIN      
        1 ctataaaaat gccgtctgaa acggtttcag acggcatttc gatgtcggcg gcggctttgc
       61 ggaatcagcc tttgaagcgt ttgaagacca gcgtgccgtt ggtgccgccg aagccgaagg
      121 agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc gttggcgcag tagtccaaat
      181 cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt cggcgggatt ttgccgtcgt
      241 gtatcgccaa aatgctgtac acggcctcca cgccgcccgc cgcgccgagc aggtggccgg
      301 tcatggattt ggtcgagctg acgacggttt tgtaggcgtg ttcgccgaac gcgcgtttga
      361 gggctttggt ttcgttggca tcgcccaagg gggtggacgt gccgtgcgcg ttgacgtaat
      421 ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg ggtaacggca agggcggggc
      481 cttcttcgtt cggcgcggtg atatggtaag catcggaact catgccgaag ccgacgattt
      541 cggcgtagat tttcgcgccg cgttttttgg cgtgttccaa ttcttccaac accaatatgc
      601 ccgcgccttc gccgataacg aagccgtcgc ggcctttgtc ccacggacgg gaagcggtgg
      661 cggggtcgtc gttgcgggtg gagagggctt tcatcgcggc aaaaccgccc acgcccaaag
      721 tgctgattgc gccttccgcg ccgccggcaa ccattatgtc cgcgtcgccg tatttgatca
      781 gtcgggcgga attgccgatg gcgtgcgcgc cggtggtaca ggcggaaacc atcccgtagc
      841 tcgggccgcg gtagcctttg aggatggtaa cgtgtccgga aatcaggttg atcagcgaac
      901 cggggataaa gaaagggttg attttgcgcg cgccgccttc gattacggct ttgccggtga
      961 cctcgatgct gggcagtccg ccgatgccgg aaccgatgtt cacgccgatg cggtctttgt
     1021 cgaggttttc cacatcgtcc aagcccgaat cggcgattgc ctgcaatgcg gcggcaatgc
     1081 cgtagtggat gaatacgtcc atccggcgcg cttctttcgc gctgatgtat tgtccgatgt
     1141 cgaaaccgcg cacttcgccg gcgacacggc tgttgatgtc ggatgcgtca aagcgggtaa
     1201 tcgcgccgat gccgcttttg ccggcgagca gggtgtccca agcctctgcg acagtgttgc
     1261 cgacagggga aacctgacct aagcctgtaa tgactactct tctctgactc atgataacct
     1321 cgctgttggt tgtcggaatg ggggcatatg cggctgtcgt gcagatgccg tctgtaattt
     1381 gcggcagggg ttcaaacagt ttgccatata agggaaaagc ctctattgcg cggtgcagca
     1441 gaggctgttg tgtcgggcga cgaccggtta gccgttgtgg gcattgatgt agtcgatagc
     1501 cagttggacg gtggtgattt tttcggcatc ttcgtcgggg atttcgcagc cgaatgcttc
     1561 ttccaaagcc ataaccagct ccacggtgtc caaagaatcc gcgcccaagt cgtcttggaa
     1621 agaagattcg tttttcacgt cggcttcgtt tacgcccagt tgttcagcaa caattttttt
     1681 aacttgttgt tcgatgtttg acatatcagt cgttccttta tgccttgcgg caggttgttt
     1741 aagggaaata aatcggtggt attgtaccga cttttaatag agttttctat ctaatgacta
     1801 ttatatcaat atttgccgat ttgtacattt ttgggtgcgg cgggttttgt cgttcaagtt
     1861 tgacctgtgt gccgtatgtt tgacgggatt tcggttaaaa tgacggcatt tccatctgaa
     1921 gcagaaagcc ctgtcatgta tccacttgcc cgccgcatcc tgtttgcact cgatgccgag
     1981 aaagcccacc acttcacgct cgacgcgctc aacatggttt ataaattggg tctgattccc
     2041 gtaaccggca accgtaccaa acctgtaaaa ctgatgggtt tggatttgcc caaccctgtc
     2101 gggcttgccg ccggactcga taaaaacggc gaatatatag acgcattggg cgcgctcggc
     2161 ttcggtttca tcgaaatcgg cacggtaacg cccaacccgc agcccggcaa cccgcagccg
     2221 cgcctcttcc gcgttcccga atatcaaggc atcatcaacc gcatgggctt caacaaccac
     2281 ggcatcgatg ccatgattca aaacatcgaa aaaagtaaat tcagcggcgt attgggcatc
     2341 aacatcggca aaaacgctgt aacaccgatt gaaaacgctg ccgatgatta tttaatctgc
     2401 ctcgaaaaag cctacgcaca cgcaagttac attaccgtca atatttcctc gcccaacact
     2461 aaaaacctcc gcgcgctgca aggtggcgac gagttgagcg cattgcttga ggctttgaaa
     2521 aacaaacagg cacagcttgc ctctgtacac gggaaatacg tcccgctcgc cgtcaaaatc
     2581 gcccccgatt tggatgaagc acaaatcgaa gacatcgccc acgttgtcaa atccgtcgaa
     2641 atggacggca tcatcgctac caataccacc atcgacaaat caagtctcgg cagccatccg
     2701 ctcgcaggcg agcagggcgg tttgagcggg ctgcccgttc atgaaaaaag taatcgggtg
     2761 ttgaagctgt tggcagacca catcgacggc aagctgccga ttatcggcgt aggcggcatt
     2821 atggaaggca gggacgcggc agagaaaatc cgcttgggcg caactgccgt ccaggtgtac
     2881 agcggattga tatacaaagg tccggcattg gtcaaagaat gtttgaaggc tttggcgcaa
     2941 tgacgcgtcc cgcacaaaat gccgtctgaa cgcacgtttt gccgttcaga cggcattttt
     3001 atttcctttt cctgcctgcc gccctttgaa aatcccttac gcgccgccct gtttgaaaca
     3061 aagcaaacgc ccgccgcgaa ccggacaatc ttgatacgca gccaaagcaa gtccggcggt
     3121 aagttgttaa acatataaat aattgatatg aaaaaataat tttatgccgt ggtggacggt
     3181 ttgagaaaac gcaaaatatg aacggcgttg aaattcatga tgtggaaaat aaggatacgg
     3241 cggataatca aaagatttta tatttaagga tttgatatgt ttaaggacga actaaatgaa
     3301 tttatccgat tgatatccga tccggaaagt gaattggatg aatggtattt gagtgatttt
     3361 aaagatgaac atatatggga aatgcaaagt tacgaagcat tttcatgttt gcgggaagca
     3421 gtcccctatt tgtttgctta tccccgatac gggtatgaac tgttggagat aatttccgcg
     3481 ttaaaagaaa catctgatac gacagaatta ttttatgagc cgggtatcgt gcctttgctg
     3541 attgatttat ataaagaaga tagttatctg gtaaatatgg tcaaacggat attcaaatga
     3601 tgaaaggggc gcgggcgttt gcttggcgga aggcaggtaa ctcaatttga aggacgcgga
     3661 agccgatacg ttaaaaagga tgaaaaatgg aaaacgggca acggaataac cggtttcctt
     3721 tggaaaaacg aattttttat ctggagcatt cggggcggta tctgatgatt tgcgccttat
     3781 cggattattc ccaaaacaaa catactgtcg ttatggcaaa tttcatctat ccggatgaaa
     3841 aaacggattg gcggaatttg gatgatttat tcaatgagtt ggttttagag gaattgcagg
     3901 cttcatttat ggattggcat ccgactgttg aagaggcaat cagccgtcat ttggaagact
     3961 tttcgtaaca ggcgtcaatc ggaaggggcg gaatgacggg gcaggcaatc ggagtaaaaa
     4021 atgaaccttg atttaaccgc gcaaaaagtc cgtctttctt ggaaggatat tctgtggggg
     4081 tatgggaata aatacttggg ttgggctgat gtggcagctt atgcccgaaa aatgacgctt
     4141 tcagatcatg atgaacgtgt gttcaaacta tctttaatca acaaatccaa tattcttgaa
     4201 ttaaagcctg ttctggaaga tttggcttcg gaaatgaggg attattcccc taaaaattgg
     4261 ctgtacgtcc tcttaagcga tgtattccat agaaaagaag aatttgagga tcctttgggg
     4321 gaagttgaaa aaatttatgc agattttgat tatccggaag aaatagaatc atttgtcagg
     4381 tatatgccgc ccaaagacgg ttatattcct tctgcccaca cctatgaaga aaatattgcc
     4441 cggttatatt ctcactggga acactatttg aacaacggcg gagggcaggg ttaaaaccgg
     4501 caatccgatg ccgtctgaag cattatccgg ccttcagacg gcaaacggat tagagcggaa
     4561 gatttgaaag cgggggcggg ctgcattccg gaagcggcaa aacccgaacc gcccgcatcg
     4621 ttgccgataa accgataagg aaatcgtttt gccggaccgg gtgcaaatgt ttgataacaa
     4681 tcctgccgcc cggcaaaaat gctttaaaga aagcaggatt ggttaattta aaaggaaaaa
     4741 ttaaaaaatg acggttgata aggaacaagt agaaaagatg atttattctg aaaataaaca
     4801 gagtgttatt gacggtatgc tcggtatgac cttcagctcg gaggaggatg aaatcccctg
     4861 gatttccgag aagttgacgg agttgtcgaa acataaagac ttggatattg ccagattgtc
     4921 attaacctgt ttcggacatc tcgccaggat gcatgaaaat atcggcgatt gcgataaagt
     4981 gattgcttta ttgctctcaa aacaaggtga tccggatttt caaggttttg ccgaagatgc
     5041 gttagacgag atttctttat ttatatttaa aaagcgtccg taagcgcgga ttaaacctgt
     5101 caatccgatg ccgtctgaag cgttatccgg ctttcagacg acatcgaagg ttgctatgat
     5161 atagtggctt gactttaaac cagtacggca tcccctcgcc ctgtcctgat ttgaagtgaa
     5221 tccgccatca tcttcctgcc attttttcaa acggaatccg aaatgtccga caaccgcctc
     5281 gacactgccc gccgccattc cctgttcctt gcccgccagc tcgacaacgg caaactcaag
     5341 cctgaaattt tcctgcccat gttggacaag gttttgaccg aagcggattt ccaagccttt
     5401 gccgactggg acaaaatccg tgcggaagaa aacgaggaag aattggcgcg gcagttgcgc
     5461 gggttgcgcc gttatgtggt gtcgcagatt atcgtgcgcg atatcaaccg catcagcgat
     5521 ttgaacgaag taacccgcac gattacgctg tttgccgatt ttgccgtcaa taccgcgctg
     5581 gattttgcct acgcctatta tcgggatatg tacggcacgc cgatcgggcg tgatacgaaa
     5641 tcgccgcagc atttgagtgt ggtggcgatg ggcaaggcgg gcggctatga gttgaacgtg
     5701 tcttccgaca tcgatttgat tttcatctat cccgaatcgg gcgataccga cggtaggcgc
     5761 gaacgcggca atcaggaatt tttcaccaaa gtcgggcaga aactgattgc gttgctgaat
     5821 gacattaccg ccgacgggca ggtgttccgc gtcgatatgc ggctgcggcc ggacggcgat
     5881 tcgggcgcgc tggtattgag cgaaaccgcg ctggagcaat acctgattac gcaggggcgc
     5941 gaatgggaac gttatgcgtg gtgcaaaggc cgcgtgctta cgccgtatcc gaacggcatc
     6001 aaatcgctgg tgcgcccctt cgtgttccga aaatatctgg attacggcgc gtatgaggcg
     6061 atgcgtaagc tgcaccgcca aatccgcagc gaagtcggca aaaaagggat ggcggacaac
     6121 atcaaactcg gcgcgggcgg catccgcgaa gtcgaattta tcgcccagat tttccagatg
     6181 atacgcggcg gacaaatgcg cgcgctgcaa ctgaaaggca cgcaggaaac gctggaaaaa
     6241 ctcgccgaat cgggcattat gccgtctgaa aacgtcgaaa ccctgctcgc cgcctaccgc
     6301 tttttgcgcg atgtcgaaca tcgtttgcaa tattgggacg accggcaaac ccaaaccctg
     6361 ccggcttcgc ccgaacagcg gcaacggctt gccgaaagta tgggatttga tgattacgcc
     6421 gattttttga acgctttaaa tcaacatcga aaccaagtca accggttgtt caacgaaatt
     6481 ttgagcgaac ccggagaaca aacgcaaagc agcggcgaat ggcagtgggt ttggcaggag
     6541 aagcccgatg aagaagggcg gcaatgccgt ctgaaggaac acgggttcga tgccgaaacc
     6601 atcgccgcgc ggctcgacca aatccgcaac gggcataaat accgccgcct ttccgcccac
     6661 gcccagccgc gtttcgacgc gcttgtgccg ttgctggtac aggcggcggc agcgcaaacc
     6721 aacccgaccg atacattgat gcggctgttg gatttcctcg aaaatatcag ccgccgttcc
     6781 gcctatctcg ccttcctcaa cgagcatccg caaaccctgg cgcaactcgc tcagattatg
     6841 agccaaagct catgggtggc ggcgtatctg aacaaatatc cgattttgtt ggacgaactc
     6901 atcagtgcgc aattattaga tacggcgttt gattgggaca aactcgccgc cgaactttca
     6961 gacggcatca aagcctgcgg cggcgacacc gaagcgcaaa tggacaccct gcgccatttc
     7021 caacacgccc aagtcttccg cctcaccgtc caagacctcg ccggattgtg gacggtagaa
     7081 tccctctccg accaactctc cgcccttgcc gacaccgtca ttgccgccgc cctttcgtgc
     7141 gcgtgggcgg atatgcccaa aaaacaccgc gacacaccgc aattcgccat cgtcggctac
     7201 ggcaaactcg gcggcaaaga actcggctac gcctccgacc tcgacttggt ctatctttat
     7261 gacgacccgc atcccgatgc aggcgacgtg tacagccgcc ttgcccgccg cctgaccaac
     7321 tggctttccg ccgccaccgg cgcaggcagc ctctacgaaa ccgacctgcg cctgcgccct
     7381 aatggcgacg ccggtttcct cgcccacagc atcgccgcct ttgaaaaata ccagcgcgaa
     7441 aacgcctgga cgtgggaaca ccaatccctt acccgcgccc gcttcatctg cggcacaccc
     7501 gaaattcaga cggccttcga ccgcatccgc accgaaatcc tcaccgccga acgcgaccaa
     7561 accgccttgt caggcgaaat catcgaaatg cgcgaaaaaa tgttccccac ccatccgcct
     7621 gccgacagca acgtcaaata cgcacgcggc ggcgtggtcg acgtcgaatt tatcgtccaa
     7681 tacctcatcc ttgcccatgc ccgaaaatat ccgcaacttc tcgccaacta cggcaacatc
     7741 gccctcttga atatcgccgc cgactgcagc cttatcgaca aaaacctcgc cgaacaaagc
     7801 cgcaccgcct accgcttcta ccgccggcag caacacaaca ccaaactgcg cgacgcagaa
     7861 aaaaccgaag taagcggcga attgctggca cattacggca atgtcaggaa attgtggcgg
     7921 gaagtgttcg gcgaagaagc ggcaaccgcc tgaacaaaaa atgccgtctg aagcctgaca
     7981 atctgggttt cagacggtat tttcgtaccg tgccgtttta aggttgcggc agagctaaag
     8041 cgatttatcg ggaatggctg aaacccaaaa accggattcc tctttcagtt acagcagctt
     8101 gatccctttc ccttatccaa cgggggaagg ctaggatagg gtggcttgca aatatacaga
     8161 acaagggaca agagccaccc tctctccaac cctctccctc cgtacgggag ggggtggatt
     8221 ctcgcgggcg aagcccacgc tacggttagc ctttacccca gcacaaacaa ttcccgcccg
     8281 tgcgccttca gccaactttt agcattgtcg gtatgcggcg tcagcgtgtt caccaaatgc
     8341 caaaagcgcg gactgtggtc ggggtggcgg aggtggcaga gttcgtggat gcagacatag
     8401 tcggcgacgt attcgggcgt gccgatcagc cgccagttga ggcggatgcc ggtgtgcggg
     8461 cggcatacgc cccaaaaggt tttggcgttg ctcaggtctg tggcggtggg cgtcagtcct
     8521 gtttcggctg cgtgtttttc aaggcggggc agcaggtatt cgcgggcgcg ttcgttcaac
     8581 aggcggcgca ggtggtcgat ttgtgcggcg gtttcttttc ggggaagcag gatttcagac
     8641 gacgtgatac ggatatggct ttggctgtgg gtatccagct tggtctttat tccccgatac
     8701 caaatccact cgggtaagtt tgggtgggaa acaggatgca cgggcgtttt ggcaagcgtg
     8761 ttccgcaaaa tcgtttcgtt tgccgccagc cagtttgcta acgcgtggtc ttgaaaaaag
     8821 ggtgggacgt tgatgctgac cgtctgcata ttgacggggc gcagaatcag atttttcttg
     8881 gcactgcgtt tgagttcgat ttcgatgcac aaaccgtcgg aaagagtata ggtgaagcgt
     8941 ttcatagttg tgaataggtt tcagaccgga tacatcgtct gaaacaggaa ttttccatat
     9001 caggcggcaa acttcggata atatacaaaa tcaaacatct gcgctacaag gttcagccga
     9061 acaagccgcc gatatatttg ctgatggtga tggcgctgag tactgccatc aaaccgacca
     9121 caatcacgcc ggaaacggtg agccacagcg ggtgtttgta gtcgccgaca attttggttt
     9181 tgtaggcggc aatcagaatc agaccgaggg aaatcggtaa aatcaggccg tttaatgcgc
     9241 ctacgaacac cagcacctgc gccggtttgc cgatggtgga aaatacggcg gtggacacgg
     9301 cgataaaggc aataatccat ttgtttttat tgcgttcgat agacgggctg agaccggaga
     9361 agaacgacac cgaagtataa gccgcaccaa tcaccgaagt aatcgaagcc gcccaaatca
     9421 ccacgccgaa aatcagcagg ccgatgtatc ccgccgcata ttcaaacggt gtggaagcag
     9481 ggttgtcggg attgagctgt acgccttggc tgaccacgcc caaaaccgcc aaaaacaata
     9541 caatccgcat aatcgaggca atcaggatcg cccgcaccga gctttggctc acttccggca
     9601 acgccgattt gcctttgata cctgcgtcca gcagacggtg cgcaccggcg aaggtgatgt
     9661 agccgccgac cgtgccgccc accagtgtaa caatcgccat tgcatcgagt ttttccggca
     9721 taaaggtatg cacggcggca tctgccagcg gcggattcgc ctgccatgcc acataaaccg
     9781 tcagcgcaat cattacgaaa cccatcactt gggcgaattt gtccatcact ttgcctgctt
     9841 ctttaaacag aaacacaccg atggcaatca cgccgctgat cacggcaccg gtttccggtg
     9901 acagtccggt cagcaggttc agacccaagc ctgcgccgcc gacgttgcca atattgaacg
     9961 ccaaaccgcc catcacaatc agcacagcca agaaatagcc tgcgccgggc aagacctgat
    10021 tggcaatatc ctgcgcctgt ttttcggaaa cggcgacaat ccgccaaata ttgagctgcg
    10081 ccccgatgtc gagcagaatc gagagcagaa tcacaaagcc gaaacttgcc gccagtgctt
    10141 gggtgaaggt ggcggtttgg gtcagaaagc ccgggccgat ggcggaagtc gccatcagga
    10201 atgcagcgcc gattaaggca tttctgcggt ttttttgatc agacataatc gcttatcctc
    10261 tataaaattg gttgttgctg tgtttgggcg aaacctgcgg ttttagctac gcagaaactc
    10321 gctttgctcg ttttggcgaa acctgcggtt ttcagacggc ctatgaactg tttttcaagc
    10381 agaaactttg atgcctgccg ccagtagttc ctgccggatt ttttcggcaa acaccacggc
    10441 gtgcggcccg tctccgtgca gacagatgct gtcggcttgc acggcaacca ggctgccgtc
    10501 cactgctttg acctgcccgt cccgcaccat ctgcaatact tgggcgatgg cttcttcgtc
    10561 gctgtccacc tgcgcatcgg ggcggctgcg gggaaccagc gtaccgtcgg gcatatagcg
    10621 gcggtcggcg aatacttcgg aaatcacacc caagcctgcg gcttttccgg cttccaagag
    10681 caggctgccg gaaagtgcca tcaatttcaa tttcgggtcg aaatccgcca caattcgggc
    10741 aacggtatcc gccagcgcac ggtttttcgc cgcttgattg tacattgcgc cgtgcggttt
    10801 gacataagcc atttccaaac cctgatcacg gcacaaggcc tgcaatgcgc ccaactggta
    10861 attcagacac gcccgcaaat cggcttcgga cagattcatt tcggtacggc cgaagttttc
    10921 ccgatcggga tagccggggt gtgctccgat gcgcacgccg ttttgttggg catacgccaa
    10981 tgccgcccga atatcggcaa tgctgccggc gtgttgggcg caggcgatgt tggccgaagt
    11041 aatcagctgc aacaaggctt cgtcgctgcc gcagccttcg gcgagatcgg cgtttaaatc
    11101 aacctgcttc atgggtgatt ctccgtattt ggttcagata ggcttggttt ttgcgccgca
    11161 gggcggtggc ttctttcaag ccgattattt tgaatttgac tttgctgccg aagcgcacct
    11221 gtgccagcct gcccaaatcg gcggcggcaa cggtagcgat tttcggataa ccgccggtgg
    11281 tttgcgcatc ggccagcagg ataatcggtt tgccgccggg cggcacctgc acggttcctg
    11341 cctgaacagc gtgggacagc atttccaaag gttgcgacag ggtcagcggc tgtccgtcga
    11401 agcggtagcc catgcggttg ctatcgcttt gcagcgtcca cgtttcccgt tccagattca
    11461 gacgcccttt ttcactgaaa gcggcatatt ccgacgaagg aacaaggtgg acggtatcgg
    11521 taaacggtat cggggcaatg ccgactttgg acaattcctg cgcacctttg ccgatgggga
    11581 gataatcgcc tttttgcagc attctgccct gatggccgcc gaaaccggct ttcaggtcgg
    11641 tacttctcga acccatcact tccggcacat caaatccgcc cgccacgcac acatagccgt
    11701 acatgccctg cacggcacgc accattttca aggtctgccc tttgcgggcg gtataacgcc
    11761 aatacgaata gaccggttcg ccgtccaatt ccgcctgata cacggcaccg gtgagacaaa
    11821 acggcgtatc ccgttcaaac accagcatta tcccgcccaa agcgatttcg attgcggccg
    11881 tgccttcgtc gttgcccaat aaaatattac ccgccgccaa agcaaccgtg tccatcgcac
    11941 cggcatgacc gatgccgtaa cgccggtgtc cgtagcgtcc ggtatcctga atatgcgccg
    12001 gtgcctgcac tgccgaaacg tgaatcatgg ctcaatcctt tctgcaacaa agcggacttg
    12061 gtcacccgcc gccagcaggg tcggcggatt caaatcggct cggaacaagg gtaattcggt
    12121 tctgccgata atctgccagc cgccgggcga agcgaacgga tacacaccgg tctgactgcc
    12181 gccgataccg accgaaccgg caggaacgga cgttctcggc acggcacggc ggggcgtgtg
    12241 caatgcttcg ggcaagccgc ccagataagg gaaaccaggc tggaagccca tcataaatac
    12301 ggtataagtt tgcgccgtat ggcggcggac gatttcggaa ataaccgtct gatggaaagc
    12361 agcgacttcc gccaaatccg ggccgtattc gccgccgtag cagacgggaa tttccaccag
    12421 tttgccctga tggtctgtaa cggcggtgtg ttcccacaca tattgcaatt catcggcaag
    12481 cgtcgccaaa tcggtatcga aacgggtgaa cacggtcaga ttgttcatgc cgaccaccac
    12541 ttcctcaatc ctgtcgtgct gcccgagcgc agcggcaaac gcccacaact tttgctgttt
    12601 gcccagttcg gaaggtgcat tcagtcggca gaccaaagcg gattcgctga ttggtgtgat
    12661 ctctattctc atttgttgtt cattttggtt atgttttaat gaatctatat gcaggggcgg
    12721 cggtttgtca atatcttctg tgctgcatca tcaaaccgtc gattggaaaa gtgctgccct
    12781 gccgctgcac tttttcagac gaccttaaac cgtttctatt aaaatagcgc attccacttt
    12841 tcagacggca tctctatgtt tcccgaccaa tccgccccca acctgctgca aggcttgaac
    12901 cctgaacaac tttccgccgt aacctggccg ccgcaatccg cccttgtgct ggcaggcgcg
    12961 ggcagcggca aaacgcgcgt gctgaccacg cgtatcgcat ggcttttgca aaccggtcag
    13021 gcaagcgtgc acagcattat ggcggtaacg tttaccaaca aagccgccaa agaaatgcaa
    13081 acccgactcg gcgcgatgat tcccgtcgac gtccgcgcta tgtggctcgg cacgttccac
    13141 ggcctctgcc accgctttct acggctgcac caccgcgatg ccggtttgcc gtcttccttt
    13201 caaatcctcg acggcggcga ccagctttcc ctcatcaaac gcctgctcaa aagcctcaac
    13261 atcgccgaag aaatcatcgc gccgcgttcg ctgcaaggct ttatcaacgc gcaaaaagaa
    13321 tccggtttgc gcgcttccgt cttgagtgcg cccgatccgc acacaagccg catgattgaa
    13381 tgctacgccg aatacgacaa aatctgccaa cgcgaaggcg tggtcgattt tgccgaactc
    13441 atgctccgca gctacgaaat gctgcaaagc aacgaaatcc tgcgccagca ttaccaaaac
    13501 cgcttcaacc acattctcgt cgacgagttc caagacacca acaaactgca atacgcctgg
    13561 ctcaaactca tggcgggcgg aaacgcagcg gtatttgccg tcggcgacga cgaccaaagc
    13621 atttaccgtt tccgtggcgc aaacgtcggc aacatgaccg cgctgatgga agaattccac
    13681 atcgacgcgc ccgtcaaact cgaacaaaac taccgctccg tcggcaacat ccttgccgcc
    13741 gccaatgccg tgattgaaaa caacgacgaa cgactcggca aaaacctgcg caccgacgcc
    13801 gaagcaggcg acaaaatccg ctactactcc gcctttaccg acctcgaaga agcccggttc
    13861 atcttggacg aaaccaaagc cctcgaacgc gaaggctggg atttggacga aatcgccgtc
    13921 ctctaccgta gcaacgccca atcccgcgtt atcgaacaaa gcctgttccg cagcggcatc
    13981 ccctacaaaa tctacggcgg cttgcgcttt tacgaacgcc aagaaatcaa acacgcgctc
    14041 gcctacctac gcctcgccgt caatcccgac gacgacaacg cccttttgcg cgtcatcaac
    14101 ttcccaccgc gcggcatcgg cgcacgcacc gtcgagaacc ttcagacggc ctcaaacgaa
    14161 caaggcatca ccctttggca ggcagcctgc aacgccggcg cgaaagccgc caaaatcgcc
    14221 gccttcgtcc gcctgattga agcactgcgc aaccaagtcg gacaactgcc cctgtccgaa
    14281 atcatcgtcg gcatcctcaa agacagcggc ctgaccgaac actaccgcac ccaaaaaggc
    14341 gacaaccaag accgtctcga caaccttgac gaactcgtca atgccgccat cgagttcaaa
    14401 cccgaagaca gcaacttcga aatcctgcct gaaaacattt cagacgaccc agccttcccc
    14461 atcctcgcct tcctaagcaa tgccgccctc gaatccggcg aaaaccaagc aggcgcgggc
    14521 gaaaaagccg tccaactcat gaccgtccac gccgccaaag gcttggaatt taacgccgtc
    14581 ttcctcaccg gcatggaaga aggccgtttc cccagcgaaa tgagccttgc cgagcgcggc
    14641 ggcctcgaag aagaacgccg cctcatgtac gtcgccatca cccgcgcccg caaacgcctc
    14701 tacatcacca tggcgcaaca aagaatgctg cacgggcaaa cccaattcgg catcgtctcc
    14761 cgcttcgtcg aagaaatccc gcccgaagta ttgcactacc tgtccatcaa aaaaaccgcc
    14821 tacgacagct atggcaacac gcgtcaaact gccacgttca aagacaaaat catcgacgac
    14881 tacaaacagc cgcaaaccta cgcaggcttc cgcatcggac aaaacgtccg ccacgccaaa
    14941 tttggcaccg gcgtgattat cgatgccgca gataaaggcg aatccgcccg actgaccatc
    15001 aatttcggca aacagggcgt gaaagagttg gacaccaagt ttgcgaaatt ggaagagatg
    15061 tagaagggag atggcagagg ccgtctgaaa acgggaacaa caatttttgc ccaaacctcc
    15121 gccgtcattc ccgcgcaggc gggaatccag acctttcggc acgggaactt atcggataaa
    15181 aggtttcttt agattccacg ttctagattc ccgcctgcgc gggaatgacg gacggtaaaa
    15241 gccgggtata gatacctaac ccttgtttat agctgaaaat caataaggaa gaacattatg
    15301 tcccaagttt ttaaagattt tgatttgtcc tccgtatggg aatccgacag ttgggcagat
    15361 gaaaactaca aagaagcccc gtttacccct gaaattttgg ctgccgtaga aagtgaactg
    15421 ggctataaat tgccgcaaag ttttattgaa ttgatggcgg tacaaaacgg cggaatattt
    15481 gtcaaaaact gttttccgac cacacagaga aattcgtggg cgaaagatca tgtgcaaatt
    15541 tgcgaggtat cgggcatcgg ctttgaaaaa gaagggagtt tgtgcggcga aacagggcaa
    15601 aaactttgga tggaagaatg ggaatatccg cccatcggcg tgtattttgc caacgacccg
    15661 tcaggcggtc atgccatgtt tgccttggac tatcgggagt gtggaaaaga cggcgagccg
    15721 aaagtggtgt ttgtcgagca agaatcggat tttgaaatcg tcgaacttgc ccccgatttt
    15781 gaaaccttta tccgcagctt gcggcatgaa gatgagttta ttgacgagga aatataaaac
    15841 ggcggttgaa aaaccggaat catcaaggga aaacgggcga aataacggat aatcgcttga
    15901 atccgtaagg aaaacggttt ggtggaacgc gccatccaag acctttgcaa agaaaaactg
    15961 tctccgacag cattgacatt attaacagaa cttatcaagt ttggagctat ctcaaatata
    16021 attcggttat ccttttgtat ccattaaatc atatgcttca attaattgtt gttctagctc
    16081 ttgtaccaat tttgaattgc gaattcctga cacaatctca aattcttctg catctatgca
    16141 aacacctgca taaatttcaa taacaaggga acgcaataat tgaagctctt ctcttgttaa
    16201 agaaataata atgtcatcac ctgtgtaatt gattatattc ataataattt tatttttgtt
    16261 tgtcaaagta agttttgcct aaggttggtc taaatgcagt tccaccatct tttgaatttg
    16321 ggtctctgat tacaattgct ccagacttat catcccaaat tgctcttatg tgtttggatt
    16381 gtaatcttcg aattcccaag aaaaaaatcg taataagttt gaaagtgtca aatcccaagt
    16441 ttcttttgag caatattcta atattttatc aatttcactt ttaataatct tatgatcaaa
    16501 ctgttctaat attaatgcat tagaccaaaa aaaaccttct ttattacaat gatgggaaat
    16561 ccatttagga gaacaaatgc aaagtgaaaa aatagatgag ccttgttctc cttcgattcc
    16621 gatatccaaa tctatccatc tatggaaatt atctggaatt tcgggggtaa atttttcaaa
    16681 atcaatatca tataaattta tgctttttaa atccaattta atcattaggg ctgtcctaga
    16741 taaataggga aattcaaatt aagttagaat tatccctatg agaaaaagtc gtctaagccg
    16801 gtataaacaa aataaactca ttgagctatt tgtcgcaggt gtaactgcaa gaacagcaac
    16861 agagcctgac agcattgttt atacggattg ttatcgtagc tatgatgtat tagatgtgag
    16921 cgaatttagc catttccgta tcaatcacag cacacatttt gctgaacgac aaaaccatat
    16981 caatggaatt gagaattttt ggaaccg
//
LOCUS       DAWEWP010000032        16598 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWEWP010000032 DAWEWP010000000
VERSION     DAWEWP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.032"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(144..566)
                     /locus_tag="WGN53_000755"
     CDS             complement(144..566)
                     /locus_tag="WGN53_000755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ0139770.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(577..1974)
                     /gene="atpD"
                     /locus_tag="WGN53_000756"
     CDS             complement(577..1974)
                     /gene="atpD"
                     /locus_tag="WGN53_000756"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ0139771.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(2012..2887)
                     /gene="atpG"
                     /locus_tag="WGN53_000757"
     CDS             complement(2012..2887)
                     /gene="atpG"
                     /locus_tag="WGN53_000757"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ0139772.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(2912..4459)
                     /locus_tag="WGN53_000758"
     CDS             complement(2912..4459)
                     /locus_tag="WGN53_000758"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ0139773.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(4470..5003)
                     /locus_tag="WGN53_000759"
     CDS             complement(4470..5003)
                     /locus_tag="WGN53_000759"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ0139774.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(5008..5478)
                     /locus_tag="WGN53_000760"
     CDS             complement(5008..5478)
                     /locus_tag="WGN53_000760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ0139775.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(5549..5785)
                     /gene="atpE"
                     /locus_tag="WGN53_000761"
     CDS             complement(5549..5785)
                     /gene="atpE"
                     /locus_tag="WGN53_000761"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ0139776.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(5842..6708)
                     /gene="atpB"
                     /locus_tag="WGN53_000762"
     CDS             complement(5842..6708)
                     /gene="atpB"
                     /locus_tag="WGN53_000762"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ0139777.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(6698..7051)
                     /locus_tag="WGN53_000763"
     CDS             complement(6698..7051)
                     /locus_tag="WGN53_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ0139778.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            complement(7419..7757)
                     /locus_tag="WGN53_000764"
     CDS             complement(7419..7757)
                     /locus_tag="WGN53_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139779.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            complement(7892..8752)
                     /locus_tag="WGN53_000765"
     CDS             complement(7892..8752)
                     /locus_tag="WGN53_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ0139780.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     VALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            8911..9480
                     /locus_tag="WGN53_000766"
     CDS             8911..9480
                     /locus_tag="WGN53_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ0139781.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            complement(9542..10405)
                     /locus_tag="WGN53_000767"
     CDS             complement(9542..10405)
                     /locus_tag="WGN53_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139782.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(10564..11250)
                     /locus_tag="WGN53_000768"
     CDS             complement(10564..11250)
                     /locus_tag="WGN53_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0139783.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVRKVLLNEARTGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            complement(11252..11989)
                     /locus_tag="WGN53_000769"
     CDS             complement(11252..11989)
                     /locus_tag="WGN53_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0139784.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            12366..14213
                     /locus_tag="WGN53_000770"
     CDS             12366..14213
                     /locus_tag="WGN53_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0139785.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETAGLSAS
                     VPTRPAEPERKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLY
                     KQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVMGAQSRVGAQGLMQVMPATAHEIA
                     GKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTPL
                     EGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            14502..14714
                     /locus_tag="WGN53_000771"
     CDS             14502..14714
                     /locus_tag="WGN53_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ0139786.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            14846..15292
                     /locus_tag="WGN53_000772"
     CDS             14846..15292
                     /locus_tag="WGN53_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ0139787.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            15313..15462
                     /locus_tag="WGN53_000773"
     CDS             15313..15462
                     /locus_tag="WGN53_000773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139788.1"
                     /translation="MPSEARIAGSDSIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(15491..15883)
                     /locus_tag="WGN53_000774"
     CDS             complement(15491..15883)
                     /locus_tag="WGN53_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ0139789.1"
                     /translation="MPTSTKPYVLRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFSGQAHDIWIPVGHVLSLFARETSEGMGFELE
                     EYRPDTPPENTPAETAPRPAKKGLKLVK"
     gene            complement(15955..16560)
                     /locus_tag="WGN53_000775"
     CDS             complement(15955..16560)
                     /locus_tag="WGN53_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0139790.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NYVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
BASE COUNT         3990 a         4389 c         4349 g         3870 t
ORIGIN      
        1 tttcggttag gcggcaggct gccctattga ataccttaaa gcaggctatg cctgccaacg
       61 ccatatccaa acacagtctt taatttaaat ccggaaaata aaaagcacga ccaaacggtc
      121 gtgcttttcc aaaccaaaca agtttatttc ttgtgcgaac ggatatagtc caaagttttg
      181 agctgtgcaa tcgcagcagc caatgcttta tgtgcttccg ccaaagcctt atcgtcttta
      241 gcttgggaaa tgcccgcttc tgcggctttt ttcgcctctt ccgcacgtgc ctgatccatc
      301 tccgcactgc ggacggcaac atccgccaag acagttactt tatcaggctg tacttccaaa
      361 acaccgccgg aaacagcaac caaaacctct ttatcctcgc ccggaacggt caaacgcaaa
      421 gcccccggcc gcaccaaact cataatcggc tcgtgtcgcg ggtaaatacc aagctcacct
      481 tgaacggtcg gaaccactac aaagctggct tcgcccgaat agatgttttg ctcgctactg
      541 accacctcaa cttgcatgat gctcatgccg acctcctcag tttaaggttt tcgctttctc
      601 aaccgcttct tcgatgctgc cgaccatgta gaatgcctgt tcgggcagat ggtcgtattc
      661 gccgttcaag atggctttga agccggcaat ggtatcgcgc agggcgacat atttgcccgg
      721 agaacctgta aacacttcgg caacgtggaa cggttgggac aggaagcgtt ggattttacg
      781 cgcacgcatt acggtcagtt tgtcttcgtc agacaattcg tccataccca agatggcgat
      841 gatgtcgcgc aattctttgt atttctgcag ggtggactgt acgccgcgcg ccacgtcgta
      901 gtgctcttga cccaagacca tcggatccag ttggcgcgaa gtggaatcaa gcggatcgac
      961 tgccgggtaa atacccaaag aggcaatatc gcggctcaat acgacggttg cgtccaagtg
     1021 ggcgaacgtt gttgccggag acgggtcggt caagtcgtcc gcaggtacat atacggcttg
     1081 gatagaagta atagaaccgg tttgggtaga ggtaatacgc tcctgcaaac gacccatttc
     1141 ttctgccaat gtcggttggt agcccactgc agacggcata cggcccaaca atgcggatac
     1201 ttcggtaccg gccagagtgt aacggtagat gttgtccacg aagaacaata cgtcgcggcc
     1261 tttgccgttt tcgtcttttt cgtcacggaa gtattccgcc atggtcaaac cggtcaatgc
     1321 gacgcgcaaa cggttgcccg gaggttcgtt catctgaccg taaaccattg ccactttatc
     1381 caatacgttg gaatctttca tctcgtggta gaagtcgtta ccttcgcggg tacgctcacc
     1441 cacgcctgcg aacacggaca agccgctgtg cgctttggcg atgttgttga tcaattccat
     1501 catgttcacg gttttaccca caccggcacc gccgaacaga cctactttac cgcctttggc
     1561 aaacggacac agcaagtcaa tcactttaat gcccgtttcg agcaattcgg ttgtggaaga
     1621 cagttcgtca aacttaggag cggcttggtg gatggcacga ctcttgtcgg tatcgatcgg
     1681 acctgcctcg tcaacaggcg ttcccaatac atcgacaatg cgtcccaacg tacctttacc
     1741 taccggcaca gtaatgggcg caccggtatt gctcacagtc atgccgcgtt tcaaaccgtc
     1801 cgagctgccc atcgcaatgg tacggactac gccgtcgccc aaaagctgtt ggacttccaa
     1861 agtcagaccg ttttcgtcta atttcaaagc gtcgtaaacg cgcggaatca tgtcgcgtgg
     1921 aaattccacg tcaacaaccg caccgataat ttgtacgatt ttgccttggc tcattatcgt
     1981 atcctaattt ccgtacagga ttcagacggc atcagacagc cgccgcacct gctacaattt
     2041 ctgacaattc cgtggtaatc gcagcttgac gcgatttgtt atataccaaa cgcaactctt
     2101 tgatggcatt gcctgcattg tctgttgcag ctttcatggc aaccatgcgg gctgcctgtt
     2161 cggatgccat attgtcgctc aacgcctgat aaaccacaga ctctaaatag cggcgaacca
     2221 gatattccaa cactgcaagt gcagtcggtt cgtagcggta ttcccagctg aacggtgatt
     2281 tgggagctga atcgccaatc acgttctcac cgataggcag caatacttcc attctcggtt
     2341 cctgacgcat ggtattgaca aaacccgaat acaccagatg gattctgtca atttcatgtt
     2401 tctcataccg ttggaagagt tctgtcaaag gtccgagcag catttccatt tttggggtat
     2461 cgcccaaatt tacggcactg gcaaccacat tcagaccaat gctctgacac gccatcagac
     2521 ctttactgcc aaggcatacg atttcctctt caataccttg attccgatac tcttgaactt
     2581 gtgccaaaaa ctttttcagc acgttggcgt tcaaaccgcc acacaaaccc ttatcagacg
     2641 taatcaaaat aaaaccgaca cgtctgattt cccgatgaga ttccagcaac ggaataccat
     2701 gatcggtatt ggtttgcgca agatggctca tcaccatacg cactttttcg gcatacggac
     2761 gcgccaaacg catccgttcc tgagtcttcc gcattttaga ggttgacacc atctgcatcg
     2821 ctttagtgat cttttgggta ttctgaacac tgcggatttt ggtgagaatc tcttttccta
     2881 ctgccatttc agactccttt cacttcaagc cttatgcctg ataggcgtaa gaagatttga
     2941 aggatttcat ggctgcttca agcgttttct cgctctcgtc ggacattgca cctgaagcat
     3001 tgacggcttc caaaacttcc ggatgttggg tacggacaaa gctcaaaaat tcagattcaa
     3061 aagccagagc tttggcaacc ggaacatcag aatacgaacc gttgttgatt gcccaaaggg
     3121 tcaaagccat ttcagccgta ttcaacgtac tgaactgttt ctgtttcatc agttcggtta
     3181 cgacttcgcc atgctccaat tgtttgcgcg tagcttcatc caaatcggat gcaaattgcg
     3241 agaacgccgc caattcacga tattgcgcca acgccaaacg gataccgcca cccagctttt
     3301 taatcacttt ggtttgtgca gcaccgccta cgcgggatac ggaaataccg gcattgattg
     3361 caggacggat accggcgttg aagaggtcgg tttccaagaa aatctgaccg tcggtaatcg
     3421 aaatgacgtt agtcggaacg aaagcagata cgtcgcccgc ttgggtttcg ataatcggca
     3481 aagcagtcag agaaccggtt ttgcctttta cttcgccgtt ggtcaatttc tccacttcgt
     3541 gttcattgac acgtgctgca cgttccaaca gacgggagtg caggtagaac acatcgccgg
     3601 gataggcttc gcggccgggc ggacggcgca aaagcaggga aatttgacgg taagccacag
     3661 cctgtttgga caaatcgtca taaacaatca aggcatcttc gccgcggtcg cggaagaatt
     3721 cacccatcgt acaaccggag taaggtgcga tatattgcaa tgccgccgct tcagatgcag
     3781 ttgcagcaac cacaatcgtg tgttccatcg cgccatgctc ttccaatttg cggaccacgt
     3841 tggcaataga agatgctttt tgaccgatag cgacataaat acagataacg cccgtacctt
     3901 tttggttgac gatggcatcc aatgctacgg ccgttttacc ggtttgacgg tcgccaataa
     3961 tcaactcacg ctgaccgcga ccgacaggaa ccatagagtc aatcgccttc agaccggttt
     4021 gcatcggctg gtcaaccgat ttgcgcgcaa tcacgcccgg tgcgattttt tcgatagggg
     4081 cggtcaaagt tgtattaatc gggcctttgc cgtcaatcgg acgacccaat gcatcaacga
     4141 cgcgtccgac cagttcgcgt ccgaccggca cttccaagat acgaccggta caagtaaccg
     4201 tgtcgccttc tttaatatgt tcgtactcgc ccaacactac ggcgccgacg gagtcgcgct
     4261 ccaggttcat cgccaagccg aaagtgttac ccgggaattc gagcatctca ccttgcattg
     4321 catctgacaa accatggatg cgaacgatac cgtcagttac cgaaatcacc gtaccacggg
     4381 tacgcacttc ggcatttaca gacagatttt cgatcttggc tttaatcaaa tcgctaattt
     4441 cagcaggatt aagctgcatg aaaactctcc taattcgtca tagtcgtgta caaggcactc
     4501 aatttgcctt gtacagacaa atccaaaacc tgatcaccca cttcaacttt tatgccgcca
     4561 atcagctccg gttcgatttc gacagagatt ttcagctcgc tgtcgaaacg cttattcagc
     4621 atttgcgcca actcgccgac ctgtttgtcg gtcaacggat aggcactgta aatgacggca
     4681 gatttgatat ggttgaatga taaggtcaag tcttgatatt gagcatatac ttccggcaat
     4741 atcgacaaac gtttctgccc ggccaagacg ataacaaagt ttttcaactc cttgtctttc
     4801 aaaccgacca aatcgatgag gatatctgct ttttctgaag cattcgtttc aggacggtca
     4861 atcaatgaag ccaccttccc ttcctgaaca accgccgcaa gtttttccag tccgcccaac
     4921 caagactcaa tttggctttt ttcctgagcc agaccgaaca atgcctttgc ataaggtctg
     4981 gcaatcgttg cgaactctgc cataagatta cagctcctgt ttcagggtat cgagcagttt
     5041 tgcgtgtttg gaagcatcga cttcgctgcg caaaatagat tcggcacctt tgacagccaa
     5101 cacggcaacc tgctcgcgca gggattcgcg tgcgcggaac aattcctgct ccacatcggc
     5161 ctttgcctga gctgcaatgc gcgccgcctc ggaagaagcc tgttctttgg cttcttcgac
     5221 aattttggcg gcacgttttt cggcgttggc aaccatttcg gaaacctgat tacgcccttc
     5281 tgccaagagt tctgcaacct ttttttcagc ctgctcgaaa tcgcttttac cacgctcggc
     5341 ggcagccaag ccttcggcga cttttgcggc acgctcatcc aaagcttttg caatcggcgg
     5401 ccacacgaat ttcatggtaa accataccaa accgaaaaag acgatgattt gagcgaataa
     5461 tgttgcattg atattcacgt tacttaacct tcgtactggg gttaatcaaa caggctgcgc
     5521 ctgtacggaa cggacgaatc cgtcctgatt atgcacctgc aaacgggtta acgaaggcga
     5581 acagcagtgc aatggcgaca ccaatcaaga atgcggcatc aatcaaaccg gcaatcagga
     5641 acagtttggt ttgcagcgga ccgatcagtt cgggctgacg ggcagaagac tccaaatatt
     5701 tagaaccgac cattgcgata ccgatagagg cacccaatgc acccaatgca acgatcaaac
     5761 cacatgcgat agcaatcaaa cccattttaa actccttaaa gaaacaaagg ttaaactaca
     5821 aaaacaaact acttaggaaa atcagtgcgc atcatgtgcc tgtccgatat agacgaacgc
     5881 caacgccatg aaaataaacg cctgcagggt aatcaccaaa atatggaaaa tcgcccatgc
     5941 caaaccggca ataatgtgga atacaaacag aatcggatcc atgacttcga cgctgccgga
     6001 agccgcccaa gcaccgccaa gcaaggctat caacaagaat accaattcgc ccgcatacat
     6061 attgccgaac aaccgcatac cgtgggatac ggttttagaa agaaactcga ccaaattcaa
     6121 cagaaagttc gcaggtgcga gttttgcacc gaacggcgcg ctgaacaact cgtgaaacca
     6181 gccacccaat cctttgattt tgatgttgta atagatacaa atcagcaaca cgccgacagc
     6241 gagtgccaaa gtggtgttca aatcggcagt cggtacgaca cgcagcaggg cgtgatggtt
     6301 gccggtaatg ccctgccata ccatcggcag caaatcgacc ggcagcatat ccatcgcgtt
     6361 catcagaaaa atccagacaa acagcgtcag acccaacggc gcgacggctt ttctagactt
     6421 ttcgttgtga atgatgctct tacacatatc gtccacaaac tcaaacaaga tttccactgc
     6481 ggcctggaaa cgtccgggaa cgcctgccgt cgcttttttt gcaccgcgcc acaacagaaa
     6541 gctgccgatt acgcccaaca ggacggcaaa aaagacggca tcaaggttaa taaacgaaaa
     6601 atcagcaatg tttttcagtc cctgaccctg agtaacatcc gacaaactgg tcaagctctg
     6661 caagtggtgc ttgatgtagt cggcagcggt aatggtttca cctgccataa tctttcactc
     6721 tcaacaatac taaaaaaacc aaatggctga caccgagcag ccccatcaga aacggggcga
     6781 acaccagcga ttgatgccat attgcaaata cggcaagcat ggacaacagc gacagcacta
     6841 cttttaaaat ctctccgaag acgaacatcc tgctttgcag gaaggggttt cccctgaaaa
     6901 gttttaaaag taaaactgca acaaacgtgg gaagcaggta ggacaaaccg ccaccgaccg
     6961 ccgaaaggaa tccggcaaaa ccccatacag caaaggcaac tgcggcgcat atggacaata
     7021 cggcggattg taggatgata atctgcttca taaagggaat gtttccgcct cggatttggg
     7081 gcgcggctaa tataatttag aagccttatt acgtcaagcg acagttaatc tttgtgaaac
     7141 aacgtatccc aatccgccgc gctcgccgcc tgaataacgg cgacaggtgt cattctaaca
     7201 cacattacat ataattacag gatattaagg agtttgtccg caatttcttt acatttttaa
     7261 tgttcttacg tgatttgttt gctttacgtg gaaataataa aaaatcaacg cgaaattgta
     7321 gcagtttatc ggtcggattg tcggcagttt ggggaatttg ctcaataaat aaaaggtcgt
     7381 ctgaaaatat tttcagacga ccttttccga ataaaggatt agcaacggcc tgccgcttta
     7441 agcaaagcat tgcattgact tttgcctttg tgcgttccgc ctcccaaaca aattgcatcg
     7501 gaagtggtaa cgccgattgt gctgattaca ctggtaacat agcattggct cacgcgctta
     7561 cccacagttg cggtaaagtt gatgcgtatg ccttcattgt tgcggttgct gatttttacg
     7621 gcatttgggc tgacgcccaa ggcaaacgcg gcacgttcct gaagtttctg gtcggaaacg
     7681 gttacattat tgattgagcc gcaacctgct aatgccaacg caacgaacgc agccgaaacg
     7741 atgatgcgtg tgttcataat ttcctcgaaa attaaaaatg aaaacaggaa aacgattctt
     7801 acgtgaagca gaaaaatgtc aatagaatta tatttcccac ttaaaatctg gaaagctgtt
     7861 ctctatattt cagacggtat atcccgcaaa attaaggtcg gtaatctatg cccaactgct
     7921 tcaagatatg gtcgaaggtt tcgggcgtgt cgaaatgcag gatgattctg ccttttttat
     7981 gattggcggt tttgatttcg gcgttgacac ccagtttttc agtcagcaaa tcattcaggc
     8041 ggccgatgtc ggcggcggca gtctttttgg gctcgggacg tttgttttga agggcgacct
     8101 ggctgcggcg ttcgacttcg cgcaccgacc agccgttttt gacggccttt tgcgccaatt
     8161 cgagctgttc gacgacgggc agggtcagca atgcgcgggc gtgccccatt tcgaggcggc
     8221 gttggtaaag catttcctgc acgggttcgg gcaggcttaa aaggcgcagg ctgttggaaa
     8281 tcgcgcttcg gcttttaccg acggcttggg cgatggtttc gtgggtcagc ccgaactcgt
     8341 cggcaaggcg tttcaagcct tgtgcttctt cgatggggtt gaggttttcg cgctggaggt
     8401 tttcgatcaa acccattgcc aacgcggttt cgtcgctgat ggttttgata acggcgggga
     8461 tttcggtcag gccggcaatc tgtgcggcac gccaacgccg ctcccccgca atcagttcgt
     8521 atcgggacag tccgtgttcg cgcacgatga cgggctgtat cacgccttgc gccttaatcg
     8581 aatccgccag ctcctgcaag gcttcgtcat cgatttgaac acgcgcctga tagcggccgg
     8641 gccggatatc tttaaccgca accgtggtca atcggtcgcc gctgctgttg tctgcgccgt
     8701 tggcgagcag cgaatccaaa ccgcgcccca atccgccttt tacttttgcc ataccgccct
     8761 cccgtgccta tttcagatag gatgttaaat cggatatttt atcggatatt gggtgttgcc
     8821 gacaatttgt atccgtgttt atcggatttc tgttttttca ctataatagc cgatttgccg
     8881 ttgcaggcgg ttttatggga aaggcggatg atggtacggc gtttgataat cggcatcagt
     8941 ggggcgagcg gtttccaata cggcgtgaag gctttggaac ttttacgcgc gcaagatatc
     9001 gaaacgcacc ttgtggtatc gaaaggtgcg gagatggcgc gcgcttcgga aacggcttat
     9061 gcgagagatg aggtatatgc cttggcggac ttcgtgcatc cgatcggcaa tatcggggcg
     9121 tgcattgcca gcggtacgtt taaaacggac gggatgctgg tcgccccctg ttcgatgcgg
     9181 acgcttgcct cggtcgcgca cggcttcggc gacaacctct tgacgcgtgc ggcggatgtg
     9241 gttttgaagg aaaggcggcg gctggtgctg atggtgcgcg aaacgccgct gaaccttgcc
     9301 catttggaca atatgaagcg ggtaacggaa atgggcggcg tggtgtttcc ccctgttcct
     9361 gcgatgtacc gcaaaccgca gacggcggac gacatagtgg cgcacagtgt tgcacacgct
     9421 ttgtcgctgt tcggaatcga tacgccggat tcggcggaat ggcagggaat ggcggattaa
     9481 aggacaaaaa tgccgtctga acacggatac ggttcagacg gcatcatttt atacgactgc
     9541 cttatttggc tgcgccttca ttccatgcgg caggggattt gtagccctcg aagcgtttgt
     9601 gcgcgtaggc tttgaacgcg tcggagttat aggcctcggt tacgtcttta agccattggc
     9661 tgtctttgtc ggcggttttg acggcagacc agttgacata ggcaaagctc ggttcttgga
     9721 acagggcttc ggtcagcttc atgccgctgc ttatggcgta gttgccgttg acgacggcaa
     9781 aatccacgtc ggcgcggcta cgcggcagtt gcgcggcttc aagctcgacg attttgatgt
     9841 ttttcaggtt ctcggcgatg tccgctttgg atgcggtcag cggattgatg ccgtctttga
     9901 gtttgatcca acccagttcg tcgagcatca ccaagacgcg ggcgaagttg gacgggtcgt
     9961 tgggcgcgga tacggtgctg ccgtctttga cttcttccag cgatttcagc ttgcccgggt
    10021 acagtcccaa aggcgcggtc ggcacttgga agacttcggt gatgtccaga ttgtgttctt
    10081 ttttgaagtc gtcaagatag ggtttgtgtt ggaagacgtt gatgtccaac tcgccctcag
    10141 ccaatgccag attcgggcgt acatagtcgg taaactcgac cagtttgacg gtgtagcctt
    10201 ttttctccag ctcggcttgg atttgttctt tgaccatatc gccgaagtcg ccgacggtcg
    10261 tgccgaagac gatttctttt ttctccgcgc cgttgtcggc ggcggcagaa gcggatgcgg
    10321 cgggcgcgct gtctttttga ccgccgcagg cggcgaggat gagcgcgagt gcggcggcgg
    10381 aaagggtttt gaagaaggtt ttcatatttt ctcctgatgt tgtggcagtt tcaaacaaaa
    10441 atgacgggca gggagtcctg ccgtccggat tcggcgttca gacggcattt gccgcgaaca
    10501 gggggatttt atagcatttt tcggatagcg gtgggggttt tggcgttcag acggcattcg
    10561 ggtttaacgt ttgtcgagtt tccgcgccaa cgcgttgccg gtgctttgaa tcaggatgac
    10621 cagcagcacg aggagggcga cgatgaagac gatgacttcg gtttggtagc ggtagtagcc
    10681 gtagcggatg gcgaggtcgc ccaagccgcc gccgcctatc atccctgccg ccgcgctgta
    10741 tgacaaaagc ccgatggcaa gcacggtaat gctggaaacc atgcccgtgc gcgcttcgtt
    10801 taagaggact ttgcggacga tggcaaccgg cggcgcaccc atcgcggcgg cggcttcaat
    10861 cacgcctttg gggacttcgc gcaggttttg ttccaccagt cgggcgaagt aaaacagacc
    10921 ggacacgctc aataccagcg aggcggcaac tggaccgatg gtgctgccga caatggcgcg
    10981 tgtggcgggt atcatcgcaa tcatcaaaat gacgaagggg aaggcgcgca tgaggttgac
    11041 gaggttgtcg agcaggaaat ttaccggctt gttgtaatgc agttggcggc tggaggttac
    11101 gaagagcaac acgcccagca acgtgccgaa aacgacggcg aatgtggtgg acaaaccgac
    11161 cattacgaag gtttcgccca atgcgcggac gatttcgcct ttcataccga tgatgttgga
    11221 aacggcttgt tggaaggtca ggtctgccat ttcagtcctc tctgatgagt tcgcgcccga
    11281 tgtcggatcg ggcgtggatt tggttgccgc gtacttcgac gatttcgacg actttgcctt
    11341 tatccaacag ggcggcgcgg tcgcacagtc gtctgataac gctcatttcg tgggttacga
    11401 tgacgatggt tacgttgaat cgtttgttga tgtcttccaa acattccaag acgctgcgcg
    11461 tggtggcggg gtcgagggcg gaagtgggtt cgtcggcgag gatgacttgg ggtttgggcg
    11521 cgagtgcgcg ggcgatgccg acacgctgtt tctgcccgcc tgaaagctgg gcgggatagt
    11581 ggccggcgcg ttcggtcagt ccgacgattt cgagacattc ggcaacgcgc gctttgattt
    11641 tttcagacgg ccatccggcg atttccaatg ggaaggcgac gttgccggcg acggtgcggt
    11701 tgttcaacag gttgaactgc tgaaacacca tgccgatatt ctgccgagcc tgacgcaatg
    11761 cggcggcatc gagcgcggtc agctcttgtc cgcagacgtt gaccttgccg ctgtcgggac
    11821 gttccaacag gttaatcagg cgcaacaggg tggatttgcc tgcacccgaa taacccatca
    11881 gcccgaagat ttcaccgtcg cggatttcga ggctggtcgg ctcgacggcg gcaaaacggg
    11941 tcttgtcgcg cgtttggtaa tgcttggaaa ccttgtccaa aataatcatt gtctttccca
    12001 tacaacaaag cccgatgtcg gacacaacgg gcgcggaaga taaagctgaa attgtcggaa
    12061 cgctttagct gttatgcccg caagctgtgt caaatcggca ggttaatttt cgtaggatat
    12121 tatcgggaaa gcgttttttt gtcaataagg caggaagcgg gcaaccattt cggacaatgc
    12181 cgtctgaaac aggcaaaggc agcagttcgc accaaaacgg caaatgattg aaaaatataa
    12241 attatgtcag aaacattcca aagccggtgc agtttcggca tataattcat gcaaacgcct
    12301 gttcagacgg cattttgtct tttccaaccc tgaccgttca gggttccgat tcttaaggaa
    12361 atccgatgta cctaccctct atgaagcatt ccctgccgct gctggcggcc ctggtgcttg
    12421 ccgcgtgttc ttcgacacac gcgcccagca gcaagaccct ggcagacaat atagaaactg
    12481 ccggtctttc ggcaagcgtt cccacccgcc ctgccgaacc cgaaagaaaa acgctggcag
    12541 attacggcgg ctacccgtcc gcactggatg cagtgaaaca gaaaaacgat gccgccgtcg
    12601 ccgcctattt ggaaaacgcc ggcgacagtg cgatggcgga aaatgtccgc aacgagtggc
    12661 tgaagtcttt gggcgcacgc agacagtgga cgctgtttgc acaggaatac gccaaactcg
    12721 aaccggcagg gcgcgcccaa gaagtcgaat gctacgccga ttcgagccgc aacgactata
    12781 cgcgtgccgc tgaactggtt aaaaatacgg gcaaactgcc ttcgggctgc accaaactgt
    12841 tggaacaggc agccgcatcc ggcttgttgg acggcaacga cgcctggagg cgcgtgcgcg
    12901 gactgctggc cggccgccaa accacagacg cacgcaacct tgccgccgca ttgggcagcc
    12961 cgtttgacgg cggtacacaa ggttcgcgcg aatatgccct gttgaacgtc atcggcaaag
    13021 aggcgcgtaa atcgccgaat gccgccgccc tgctgtccga aatggaaagc ggtttaagcc
    13081 tcgaacaacg cagtttcgcg tggggcgtat tggggcatta tcagtcgcaa agcctcaatg
    13141 tgcctgccgc cttggactat tacggcaagg ttgccgaccg ccgccaactg accgacgacc
    13201 aaatcgagtg gtacgcccgc gccgccttgc gcgcccgacg ttgggacgag ctggcctccg
    13261 ttatctcgca tatgcccgaa aaactgcaaa aaagcccgac ctggctctac tggctggcac
    13321 gcagtcgcgc cgcaacgggc aacacgcaag aggcggaaaa actttacaaa caggcagcag
    13381 cgacgggcag gaatttttat gcggtgctgg cgggggaaga gttggggcgc aaaatcgata
    13441 cgcgcaacaa tgtgcccgat gccggcaaaa acagcgtcct ccgtatggcg gaagacggcg
    13501 cgattaagcg cgcgctggtg ctgttccgaa acagccgaac cgccggcgat gcgaaaatgc
    13561 gccgtcaggc tcaggcggaa tggcgttttg ccacacgcgg ctttgacgaa gacaagctgc
    13621 tgaccgccgc gcaaaccgcg ttcgaccacg gtttttacga tatggcggtc aacagcgcgg
    13681 aacgtaccga ccgcaaactc aactacacat tgcgctatat ttcgccgttt aaagacacgg
    13741 taatccgcca cgcgcaaaat gttaatgtcg atccggcgtg ggtttacggg ctgattcgtc
    13801 aggaaagccg cttcgttatg ggcgcgcaat cccgcgtagg cgcgcagggg ctgatgcagg
    13861 ttatgcctgc caccgcgcac gaaatcgccg gcaaaatcgg tatggatgcc gcacaacttt
    13921 acaccgccga cggcaatatc cgtatgggga cgtggtatat ggcggacacc aaacgccgcc
    13981 tgcaaaacaa cgaagtcctc gccaccgcag gctataacgc cggtcccggc agggcgcgcc
    14041 gatggcaggc ggacacgccc ctcgaaggcg cggtatatgc cgaaaccatc ccgttttccg
    14101 aaacgcgcga ctatgtcaaa aaagtgatga ccaacgccgc ctactacgcc gccctcttcg
    14161 gcgcgccgca catcccgctc aaacagcgta tgggcattgt tcctgcacgc tgacgtaccg
    14221 atgccgtctg aaacccgccc ggtctttcag acggcatttt tatcccgaac ggcattgacg
    14281 gcgaaccata aatataagac aatccgaaaa ttgtttttcc tgctttttca agcagcttga
    14341 cacggcacaa gccgacccgt taggaggtga tgtttccgtc acggcgcgta tcccgccgcc
    14401 gcaaggcaca gcgatacggt aaaccttcaa caccgtctgc cctacccttt ccaccgatat
    14461 gatgggcaga tgaaacaacc gaatttatta aaggaaataa aatgcctgca atccgcgtaa
    14521 aagagaatga accctttgaa gtcgctatgc gccgtttcaa acgcgccgta gaaaaaaccg
    14581 gcctgctgac cgagctgcgc gcccgcgaag cctacgaaaa accgactacc gaacgcaaac
    14641 gcaaaaaagc ggcagccgta aaacgcctgc aaaaacgcct gcgcagccaa caactgccgc
    14701 ccaaaatgta ctaaacgttc aagtacagat tacaggtcag ccctgtgata tgaggacaca
    14761 ccgcaagacc tgctctgcgg tgtgttttgc ttttcagacg gcatcgaaac ccgccgtttc
    14821 catccgacat cccagcgagg acattatgag cctgaaaatc cgccttaccg aagacatgaa
    14881 aaccgcgatg cgcgccaaag accaagtttc cctcggcacc atccgcctca tcaacgccgc
    14941 cgtcaaacag tttgaagtgg acgaacgcac cgaagccgac gatgccaaaa tcaccgccat
    15001 cctgaccaaa atggtcaaac agcgaaaaga cagcgcgaaa atctacactg aagccggccg
    15061 tcaggatttg gcagacaaag aaaacgccga aatcgaggta ctgcaccgct accttcccca
    15121 aatgctctcc gccggggaaa tccgtactgc ggtcgatgcc gccgttgccg aaaccggcgc
    15181 ggcaggtatg gcggatatgg gcaaagtgat gggcgtattg aaaacccgac ttgccggcaa
    15241 agccgatatg ggcgaagtca acaaaatcct gaaagccgtg ctgaccgcct gattgcccga
    15301 atatcggaca aaatgccgtc tgaagcccgt atcgcaggtt cagacagcat tttcaatgtc
    15361 ccaatatcga atcggcaggg gcaacacggt ttcgatacac cgaaacgggt tttgccgata
    15421 aacggattcc gtttgcgccc catcggacaa aatgccgtct gaaacacgat tccgttcaga
    15481 cggcatagat ttatttgacc aatttcaagc cttttttggc gggtcggggc gcggtttcgg
    15541 caggggtgtt ttcaggcggc gtatcggggc ggtactcctc caattcaaac cccatacctt
    15601 cgctggtctc ccgtgcgaaa aggctgagga cgtgtccgac aggtatccat atatcgtgcg
    15661 cctgtccgct gaaacgggcg gaaaagctga tccaatcgtt gtcgatttga aggttttgcg
    15721 tggcggttgc gccgatattg agcataattt cgttgtcgcg gacgtactgc atggggacgc
    15781 gcgtgtgttc gttgacccaa acaaggatgt gcggtgtgag gctgttgtcg ctgcaccatt
    15841 cgcagagagc gcggaggacg tagggtttgg tggaagtggg cataatgggt tccgtgttgt
    15901 acgccaaaat aggaaaatgc ctgcaaaacg gtgggttttg caagcatttc ggacttattt
    15961 gcgcatggct ttttcggcgg gtgtcagtgc ttcgataaag gcttcgcgct ggaagatgcg
    16021 ctcggcgtat ttgagcagcg gcgcggcact tttgcccagt ttgacatcgt agtggtcgag
    16081 ccgccacagc agcggagcaa gggcgacatc aatcatagaa aaatcttcgc cgaggatgta
    16141 tttgcttttg ctgaacgaag gggcgagcat cgtcaggccg ttgccgatgg cttcgcgcgc
    16201 ttttgcctgt tccttgttgg tggcggcggg gttttccaaa acttggacgt agttgaacaa
    16261 ttctttttcc atacggtaca gcaccagccg gcccctgccg cgcataacgg gatcaccggg
    16321 cataagctgc ggatggggga agcgttcgtc gatgtattcg ttgatgatgt tggactcgtg
    16381 cagcaccaaa tcgcgctcga ccagtacggg gacctggttg tacgggttca tgacggcgag
    16441 gtcttcgggt ttgttgtaaa tatcgacgtc tttgatttca aaatccatac ctttttcgta
    16501 caaaacgaag cggcagcggt ggctgaaggg gcaggtaatg ccggaataga gggtcatcat
    16561 aataattgtc gctcctgtgt gatgcctaca aaacggct
//
LOCUS       DAWEWP010000033        16541 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWEWP010000033 DAWEWP010000000
VERSION     DAWEWP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.033"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            117..1292
                     /locus_tag="WGN53_000776"
     CDS             117..1292
                     /locus_tag="WGN53_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ0139791.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAIIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKRLPEWTARRQENAAKLAESLG
                     KFASIRLVEVADYIEHAQYKFYTFVKPEHLKDGWMRDRIVNELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            1340..3250
                     /gene="pglD"
                     /locus_tag="WGN53_000777"
     CDS             1340..3250
                     /gene="pglD"
                     /locus_tag="WGN53_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ0139792.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TAHPRIMTADEAMLPWHELSVLLDRIRSACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            3306..4598
                     /locus_tag="WGN53_000778"
     CDS             3306..4598
                     /locus_tag="WGN53_000778"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ0139793.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDSEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(4650..5486)
                     /locus_tag="WGN53_000779"
     CDS             complement(4650..5486)
                     /locus_tag="WGN53_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ0139794.1"
                     /translation="MIDLHCHSIVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RY"
     gene            complement(5796..6197)
                     /locus_tag="WGN53_000780"
     CDS             complement(5796..6197)
                     /locus_tag="WGN53_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139795.1"
                     /translation="MAIYDLNEIVTSAALGMYKGKWQNMDFEQVLQDIVQETDFTYSW
                     EYGENWAIFCLNEKSVGMLCKQIPFAIIENEKLSQKIYQEYPWILTQQVYHFDTDKIL
                     LNKYVCKLTIGIMSAISQEPISLQDLYVDTV"
     gene            complement(6302..6505)
                     /locus_tag="WGN53_000781"
     CDS             complement(6302..6505)
                     /locus_tag="WGN53_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139796.1"
                     /translation="MDTTLKNKAEAILGEPLLDEPIRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(6483..6716)
                     /locus_tag="WGN53_000782"
     CDS             complement(6483..6716)
                     /locus_tag="WGN53_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0139797.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEE"
     gene            6882..10316
                     /gene="dnaE"
                     /locus_tag="WGN53_000783"
     CDS             6882..10316
                     /gene="dnaE"
                     /locus_tag="WGN53_000783"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ0139798.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVWIGNPDAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNVHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAEKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            10464..10613
                     /locus_tag="WGN53_000784"
     CDS             10464..10613
                     /locus_tag="WGN53_000784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139799.1"
                     /translation="MHDTDSTKGKNGIFQSIGIKTALSLQKYQIDKPGKEIRPCRLKK
                     RPPVR"
     gene            complement(10750..11682)
                     /locus_tag="WGN53_000785"
     CDS             complement(10750..11682)
                     /locus_tag="WGN53_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0139800.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEMPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMPNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFAAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLNGIDVCLNIGALERGSDCKRREERDMAGQAEQMAAELDRHG
                     AAVFFREYPNADHGNVPFYSLTDCVEYLRKAWQR"
     gene            complement(11752..13908)
                     /locus_tag="WGN53_000786"
     CDS             complement(11752..13908)
                     /locus_tag="WGN53_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0139801.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVCKRPAKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRHETFYAAADWDINPDTVLGAGYLYQQRHLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMNSHDVFADLKHYFGNGGYGKVGMRYSDRDADSNYAFAGSKLGMKT
                     LAGRPGCNTADDKACAVGLGTEIKQKALAFDASYSRPFRLGNTANEFVIGADYNRFRS
                     TNEQGRTTLYARGGLALNEFRSIPQVDLIANARKGVRGYSHTVATENLDEFGIYGKST
                     FHPADGLSLIGGGRLGHYKIELGEGKTLHKASKTKFTGYAGAVYDLNDNNSLYLSLSQ
                     LYTPQTNLDADGKLLKPRQGNQFEVGYKGSYMDDRLNARVSFYRMKDKNAAAPLNPNN
                     KKTRYAALGKRVMEGVETEISGAVTPKWQIHAGYSYLHSQIKTASNSRDDGIFLLMPK
                     HSANLWTTYQVTPELTIGGGVNAMSGITSSAGMHAGGYATFDAMAAYRFTPKLKLQIN
                     ADNIFNRHYYARVGGANTFNIPGSERSLTANLRYSF"
     gene            14068..14727
                     /locus_tag="WGN53_000787"
     CDS             14068..14727
                     /locus_tag="WGN53_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ0139802.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRDLLEMPSETAVADIARTYSAHYLNPNNRNMTLFPDALPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGFDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLARHY
                     A"
     gene            complement(14814..15782)
                     /gene="ispH"
                     /locus_tag="WGN53_000788"
                     /gene_synonym="lytB"
     CDS             complement(14814..15782)
                     /gene="ispH"
                     /locus_tag="WGN53_000788"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ0139803.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNACFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(15811..16308)
                     /locus_tag="WGN53_000789"
     CDS             complement(15811..16308)
                     /locus_tag="WGN53_000789"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ0139804.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT         3799 a         4789 c         4306 g         3647 t
ORIGIN      
        1 ccccgtacag accgattttg acaacacctg cggcgcgcgc ccgattcttc ggaacacgcc
       61 ccccttcaga cggcataggg tcggaaatgc cgtctgaacc cgacggacaa accatcatgc
      121 tgaacacctc cctttccccg tggccctgct tcacccaaga agaagccgat gccgtttcca
      181 aagtcctgct gtccaacaaa gtcaactact ggacgggaaa cgaatgccgc gaatttgaaa
      241 aagaatttgc cgcctttgcc ggcacgcggt acgccgtcgc cctttccaac ggcacgctgg
      301 cactcgatgt cgcgctcaaa gcaatgggca taggcgcggg cgacgatgtg attgttactt
      361 cgcgcacctt cctcgcctcc gcctcctgca tcgtgaacgc aggcgcaaac cccgtgtttg
      421 ccgacgtgga tttgaacagc caaaacatca gcgcggaaac cgtcaaggct gtgctgaccc
      481 cgaataccaa agcgattatc gtggttcacc ttgccggtat gcctgccgaa atggacggca
      541 ttatggcttt ggcaaaagaa catgatttgt gggtgattga agactgcgcc caagcgcacg
      601 gcgcaaaata caaaggcaaa tccgtcggct ctatcggaca cgtcggcgcg tggtcgttct
      661 gccaagacaa aatcatgacc accggtggcg aaggcggtat ggttacgacc aacgacaaaa
      721 ccctgtggga aaaaatgtgg tcgtacaaag accacggcaa aagctacgat gccgtgtacc
      781 accgcgaaca cgcgcccggt ttccgctggc tgcacgaaag tttcggcaca aactggcgta
      841 tgatggaaat gcaggcggtc attggccgca tccagctcaa acgcctgccc gaatggacag
      901 cacgccgcca agaaaacgct gccaaactgg ctgaaagctt gggcaagttt gccagcatcc
      961 gcttggttga agtcgccgac tacatcgaac acgcgcaata caagttctac accttcgtca
     1021 aacccgaaca cctcaaagac ggctggatgc gcgaccgcat cgtgaacgaa ctgaacgcgc
     1081 gcaaagtccc ctgctatcaa ggcagctgct ccgaagtcta tttggaaaaa gccttcgaca
     1141 acacaccttg gcggccgaaa gagcgtttga aaaacgccgt cgaactgggc gacaccagcc
     1201 tgatgttctt agtacatccg acgctgaccg acggcgaaat cgcgttctgc aaagaacaca
     1261 tcgaagccgt attgaccgaa gccacacgat aacccttcag acggcatatg ccgcctgaaa
     1321 gcacataccg ccccacgata tgactctgga aaccctgctt gccctaccgc gcaacatcaa
     1381 gaaaatctgt ttcctcatac acgattttct gatgattttc attgcctttt ggtttaccca
     1441 aagcctgaag gccgactact cgaacgaatg gttcgaccct gccaactggc agtctttcct
     1501 attgaccgcg ccgttgacca tcggcctgtt cgtcagaatg gggctctacc gtgccgttac
     1561 acgcttcatc agcttccgca tcctctccac cgcgctggca ggcagcctcg cctccgccgt
     1621 attgttcttc ctcaacacat tgatttttga agaaaggctg cgcctcgccc tgccgattgt
     1681 ctatttctta ctgctgtttg tttccgtgac cggctcgcgt atggttttgc gcggactgtt
     1741 gtccgaacac cccaaaaaac agatgatccc tgtcatcatt tacggcgcgg gacggtcggg
     1801 cagacaactg cttgaggccg tcaaacaaat gcgcgaatat tccgccgccg cctttgtaga
     1861 cgacgacccc aaactgtggc acaccgtcat ctacgacctt gccgtttacc agcccgatgc
     1921 catcgccttc ctcatcgaac gctacggcgt ggaaaaaatc ctgctcgcca tccccggcgc
     1981 gacccaggaa caacgccgcc gaatcatcaa caaactggaa gcctatccgt gcgaagtgtt
     2041 gaccattccc ggaatgaaag acctgatgga cggaaaaatc agcatcggca cgctcaaaaa
     2101 aatctctgtg tccgacctgc tcgggcgtga ttccgtcgcg cccgacgacc gcctgatgag
     2161 tgccgacatc gaaggcaaaa ccgtcatggt aaccggcgcg ggcggctcca tcggttcgga
     2221 actctgccgc cagattatcc gccgccgccc cgaaaagctg ctgctgttcg agttatccga
     2281 attcgccctg tacgccatcg aaaaagaatt gcgcgaaacc tgcatccaaa aacgcctcga
     2341 caccgaaatc ctgccctttc tcggttcggt gcaaaaccgc acgctgctcg aacacgtcat
     2401 gaccgccttt tccgttgcga ccgtctatca cgccgctgcc tacaaacacg tccccatggt
     2461 cgagttcaac accgtcgaag gcatacgcaa caacatcttc ggcacactcg agtgcgcgct
     2521 tgccgccacg acatcgggcg taagaacttt cgtcctcatc tccaccgaca aagccgtccg
     2581 ccccaccaac accatgggtg ccagcaaacg catggcggaa ctctgccttc aggcactcgc
     2641 cgccgaaccc ggacaaaaaa cccgcttcag catggtacgt ttcggcaatg ttttaggttc
     2701 gtccggctcc gttgtcccgc tgtttgaaaa acagattgca gaaggcggcc cgcttaccct
     2761 gacccacccc gaaatcacac gttatttcat gaccataccc gaagccgccc aactcgtcat
     2821 acaggcaggc gcgatgggta cgggcggcga cgtattcgtc ctcgacatgg gtgaatccgt
     2881 caaaatcatc gaccttgccc gccaaatgat taccctaagc ggcctcaaac ccaacacacc
     2941 cgaacaaccc gacggcgaca tcgaaatcct cattaccgga ctgcgtcccg gcgaaaaact
     3001 ctacgaagag ctgctcatcg gcgacaacgt ccgcaaaacc gcccatccgc gcattatgac
     3061 ggctgacgag gcgatgctgc cgtggcacga gctctctgtc ctgctcgacc gcatccgttc
     3121 ggcctgcgac cgttacgacc agcaggcaat ccgcaccctg ctcatcaacg ccccgaccgg
     3181 ctttgccccg agcgacggca tctgcgacct gctttgggta cgagaaacac acagaaaaaa
     3241 tgccgtctga accttcagac ggcataacgt acaaaccaac ctaccttaca cacacggagt
     3301 ttgacatgca gttctcagca ttcggcgaaa aattcacgca acacagcggc atcctccaac
     3361 tgatggacga cctcggcgac gcgctcaaaa gcgacaagcc cgtcaatatg ctcggcggcg
     3421 gcaatccggc gcgcattccg gaaatcgatc gggcgttcgc cgacatattc tccaaactgg
     3481 cggcggaaca cgctgtcgag aacatcggca actactccaa tccccaaggc gatgccgcgc
     3541 tgattgacgc gctgaccgcc ttcctcaacc gcgaatacgg ctggaacctg accgccgaca
     3601 atatcgcgct gaccaacggt tcgcaaaacg cgtttttcta tttattcaac ctcttcggcg
     3661 gcaaattcaa cctttcagac ggcacatccg cagaaaaagc cattttgttg ccgctcgcgc
     3721 ccgaatacat cggctatgcc gacgtgcatg tcgaagggca gcacttcgtt tccgtcaagc
     3781 ccaaaatcga aaacgtcgaa cacgaaggcg aagcaggctt cttcaaatac cgcgtggact
     3841 ttgacgcact ggaaaacctg cccgaactca aagcgggcaa aatcggcgcg atttgctgtt
     3901 cgcgcccgac caacccgacc ggcaacgtgc tgaccgacag cgaaatggcg cggctggacg
     3961 ctttggcgcg tgaacacggg attccgctga ttatcgacaa cgcctacgga atgccgttcc
     4021 ccaacatcat ttacagcgac gtaacgctga attggcacga aaacatcatc ctctgcttca
     4081 gcctttccaa agtcggcctg ccgggcgttc gcaccggcat catcgtcgcc gcgcccgaag
     4141 tcgtcaaagc cgtcagcagc ctgaacgcga ttgtgaacct tgcccccacg cgcttcggcg
     4201 cggccatcgc cgccccgctg ctggaaagcg gcgagatgaa acggcttgcc gaccaagtca
     4261 tccggccgtt ttaccgcaat caggcgcaaa ccgccgtctc gctgctcaag cgcgagctgg
     4321 gcgcgtaccc gatgaaaatc cacaaacccg aaggcgcgat tttcctgtgg ctctggtttg
     4381 aaaacctgcc cgtttcttcg caaaccctgt acgaaatgct caaagccgaa ggtacgctga
     4441 ttattccggg cgagcatttc ttcgtcggca tcgacacgcg ggattacccg cacgcgggcg
     4501 agtgcatccg catgagcatc gcgcaggacg ctcaaacgct ggaaaaaggc atcgccgcca
     4561 tcggcaaaac cgtccgaaaa ctgtacgaca acgtttaaac cgcaaaaaat gccgtctgaa
     4621 aggttttcag acggcatttt tatctgcatt caatatcggg aaaaatgcgc ccacaccggc
     4681 ctgcagtttt ccggcagttc gggacacgcg ccgagaatgc cgccgctaaa gtcgtttaaa
     4741 cggtggaagt cgctgcccgc gctggcgagc ataccgaagc gttctgccaa aagcgcgtag
     4801 ttgaggcggt cgtttttgca gcagttgccg ctgtggactt cgatgcctgc gccgccgagg
     4861 tttttaaatt cttcaaacag attgcgcttg gcggtggcgg acaaatcata acgcatcgga
     4921 tgggcgatga ccgccaaccc gcccgcgccg ttgacggcgg agacgcagtc ttccagcgtc
     4981 gcccattcgt ggcggacggc gcaggatttg ccgtcgccca agtatttggt gaacgcctgc
     5041 tgcttgtttt tgacgtgtcc cacccgaatg aggaactcgg cgacgtgtgt gcggctgacc
     5101 atttctttgt ttgccgccag cgcgagcgcg ccgtcgtatg cgcccgaaat gccgcgtttt
     5161 tccagcttgg cggcgatggc ttcgaggcgt ttcagacggc cttgccgcac ttgcgccaac
     5221 aggttttgca ggttttcgtc ttgttcgtca aagtctaagc cgacaacgtg tatggtacgc
     5281 ccgcgccacg ttacggagat ttccacgccg ttaatcaggc gcaaaccgag cctgtcggct
     5341 tcggcacgcg cttcggcgat gccgccggtg tggtcgtggt cggtcaacgc cagcagcgtg
     5401 cagccgtttt gatgcgcgag gcgcacgact tcggcggggg aaagcatacc gtcggaaacg
     5461 atggaatggc agtgcaggtc tatcatgggg tgttatgtgt gtggtgaatg aaggtcggga
     5521 tgtttcaatg ttgttgggca gtgtggcggc ggcggtcatt ttaaaaaaca ggtgtatggc
     5581 aaatgcaaag gaaaagccgc tatgccgtca ttcccaaccc ccaaatgtac ggatagagcc
     5641 agtagggatg ggtacattat ttgtttgcat tattagttat cctgctgtat ccattaaatc
     5701 atatgcttca attaattgtt gttctagctc ttgtaccaat tttggattgc gaattcctaa
     5761 cgcaatttca aacagcgagt tctcactatg tacatctaca cagtatctac ataaagatct
     5821 tgtaaagata taggttcttg gctgatggca ctcattattc caatagttaa tttgcataca
     5881 tatttgttta ataaaatttt atctgtatca aaatgataaa cttgttgagt tagtatccaa
     5941 gggtattctt ggtatatttt ctgtgataat ttttcattct ctataatcgc gaaaggtatc
     6001 tgtttacata acataccaac agacttttcg tttagacaga aaatagccca attttcccca
     6061 tattcccaag agtatgtaaa atctgtttcc tgaacaatat cttgtaaaac ttgctcaaaa
     6121 tccatgtttt gccatttacc tttatacata cctaatgcgg cactggttac tatttcgttt
     6181 aagtcataaa tagccattta tgttccttgc taatttctag gtgagacatg tataggactg
     6241 agaccagtat gcccattagg ttcccctcta tacacattaa tagtagatgt tggttccgtt
     6301 ttcagacggc atcgtccgac caacccgctt ctttcaattt ccgccgttgc gcgtcgtagc
     6361 gggccttatc cgcccaataa atcgtctgaa tgcacgcctc gccgcagtcg ctgccgcaac
     6421 attcccacga ctcggggcgg ataggttcgt ctaaaagcgg ttcgcccaag atggcttcgg
     6481 ctttattctt cagggtcgta tccatcggca atttccaagc gtgcgccgtc aaattcaatc
     6541 acttcgccgc cgcgtatttt ggcggtttta cgggtttcgg tttcgccgtt gcgcaacacc
     6601 agcccttcgg cgataaacgc ttttgcctgt ccgccgcttt cagcaagacc ggccagtttc
     6661 aacaggtcgc ataaagcgat gtattcatta tcttcaagat agactgttgc ttccatcatt
     6721 caatcctttt caataagggc gatattttaa catggccgtc tgaaagaaag cccccttctg
     6781 tttgatttgc tttatccaac cccattacat ttgattttca gacggccttt agctgccaac
     6841 cctgctacaa tacgcctttt attgttcacg ccgattttgc catgaccgag ccgacctaca
     6901 tccccctgcg cctgcatacc gaattttcga ttaccgacgg tatggtgcgg attaaaaaac
     6961 tgattgccaa agcgcaggaa tacggtttgc ctgctttggg catcagcgat ttgatgaacg
     7021 aattcggttt ggtgaaattt tataaagcct gccgcagcgc ggggattaag cccgtcggcg
     7081 cggcggacgt atggataggc aatccggatg cgcccgacaa gccgttccgt gctatgctga
     7141 ttatccgcaa cgatgcgggc tatctgcgct tgagcgagct tttgacggcg gcttatgtcg
     7201 gcaaagaccg caatgtccat catgcggaac tcaatcccga atggctggaa aacggcgaca
     7261 acagcggctt gatttgtttg agcggcgcgc attacggcga agtgggcgtg aatctcttga
     7321 acggcaacga agacgcggcg cgggcggcgg cgttgaaata tgcggcgtgg tttcccgatg
     7381 cgttttatct ggagttgcaa cgcctgcccg agcgtcccga atgggaggct tgcgtttcgg
     7441 gcagcgtgaa gctggcggag gaactgggtt tgccggtggt ggcgacgcat ccgacgcagt
     7501 ttatgagccg cgacgatttc aacgcgcacg aggcgcgggt gtgtatcgca ggcggctggg
     7561 tattgacgga caagaaacgt ccgcgcgatt tcacaccgag ccagtttttc attccgccgg
     7621 aaacgatggc cgaacgtttc gccgatttac ccgaagccct cgaaaacacg gtggaaattg
     7681 ccaaacgctg caatctgcac atcacattgg gcaaaaactt cctgcccctt ttccccacgc
     7741 ccgacggttt atcactcgat gactatctca tcaaactctc caatgagggc ttgcaggaac
     7801 gcatggttca gctttatccc gacgaggcgg agcgtgcgga aaaaatgccg gaatatcagg
     7861 aacgtttgga ttttgagctg aacatcatca tccagatgaa attccccggc tatttcctta
     7921 tcgtacaaga ctttatcaac tgggcgaaaa cacacggctg cccggtcggg ccgggacgcg
     7981 gttcgggcgc gggttcgctg gtggcgtatt cgctgaagat taccgacctc gatccgctga
     8041 aatacgcgct gctgttcgag cgtttcctaa accccgaacg cgtttctatg cccgacttcg
     8101 acgtggactt ctgtcaggca aaccgcggcc gcgtgattga atatgtacgc gaaaaatacg
     8161 gtgcggaagc agtcagccag attgttacct tcggcacgat gtcgtccaaa gcagtgattc
     8221 gcgacgtggg gcgcgtactg gaactgccgt ttatgctgtg cgacaaactg tccaagctga
     8281 ttccgttgga agccaacaaa cctttgggtt tggacgacgc gatgaaggcg gagccgcaga
     8341 ttcaggaatt aatcgaagcg gaagaagcgg acgaactgat tacgctggcg aaaaagctgg
     8401 aagatttgac gcgcggtttg ggtatgcacg cgggcggcgt gttgattgcg ccgggcaaga
     8461 tttccgatta cagccccgtg tatcaggcgg acgaatccgc ctcgcccgta tccatgtacg
     8521 acaagggcga cgtggaagac gtgggtttgg tgaaattcga ctttttaggt ctgcgcaacc
     8581 tgaccatcat cgaaatggcg cagaacaaca tcaaaaacac caccggcgac atcatcgatg
     8641 tcggcaaaat tccgcttgac gaccaggttg cctaccaaat cttccgcgat gcgaacacca
     8701 ccgccgtgtt ccagttcgag tcgacaggca tgaagaaaat gctgaaaacg gcgcatacga
     8761 ccaagtttga agaactgatt gccttcgtat cgctctaccg acccggcccg atggacaata
     8821 ttcccgactt cgtcgcgcgt atgaaaggtc aggaattcca atacatccac ccgctactcg
     8881 aaggcatcct cgcaccaacc tacgggatta tggtgtatca ggaacaagtg atgcaggcgg
     8941 cgcagattat cggcggctac tcgctcggcg gcgcggacct gctgcgccgc gccatgggta
     9001 agaaaaagcc cgaagaaatg gtgaaacacc gcgaaatctt cgccgaaggc gcggcaaaac
     9061 aaggcatttc gcgcgaaaaa tccgacgaaa tcttcaacta catggaaaaa ttcgccggct
     9121 acggtttcaa caaatcccac gctgccgcct acgccctgat ttcctaccag accgcatggc
     9181 tcaaagccca ctaccccgcc gaatttatgg cggcgaccat gtcgtctgaa ttggacaaca
     9241 ccgaccagct caagcatttc tacgacgact gccgtgccaa cggcatcgag ttcctgccgc
     9301 ccgacatcaa cgaatcagac taccgcttca cgccgtatcc gaacatgaaa atccgctacg
     9361 cgctcggcgc gattaaaggc acgggcgaag ccgccgtcga atccatcacc gccgcgcggc
     9421 aaagcggcgg caagtttacc ggcctcttgg acttctgtga gcgcgtcggc aaagaacaca
     9481 tgaaccgccg caccctcgaa gctctgatac gcggcggcgc attcgacagc atcgaaccca
     9541 accgcgccat gctcttggcg aacatcgacc tcgctatgga caacgccgac caaaaagccg
     9601 ccaacgccaa tcagggcggg cttttcgaca tgatggaaga cgccatcgaa ccggtgcggc
     9661 tcatcgacgc gccgatgtgg agcgaatcgg aaaaactcgc cgaagaaaaa accgtcatcg
     9721 gcttttacct gtccggccac ccgttcggcc cgtatgccca agaagtccgc caaatcgccc
     9781 cgcagaaact gtcgaaactc aaaccgcaag acagcgtgcg cctcgccgga ttcgttaccg
     9841 ccgtacgcac catgatgggc aagcgcggca aaatcgcctt cgtcagcctt gaagatttga
     9901 gcggacaggt tgaaatcatg gtcggcggtc agacgttgga aaactgcgcc gactgcctca
     9961 aagccgacca agtgctgatt atcgaatcca aagtcagccg cgacgactac ggcggcggcg
    10021 acggactgcg tattctggca aaccaagtca tgaccctgca aacggcacgc gaacgctacg
    10081 cccgcagcct cagcctcgcc ctcgccccgc atcacgacat cggcggactg gtacaactgc
    10141 tcgccgccca ccaactgccc gacacgccgc gcatcccgct gcaactgtcg tatgccaacg
    10201 aaaaagcgtc gggcaggctt caagtgccgc cgaaatggac ggttacaccg agctccgcat
    10261 tgttcggcga actggaaaca ttgctcggca gccggtcggt gcgcgtcaac tggtaaccaa
    10321 aaatagaaat gccgtctgaa gcccaaaaac cggtttcatt cgtactttat tcgaatgatt
    10381 gaataaaagt aactgccaag aaaaacgtat tttttggtta tttcgccagt ctaaatagag
    10441 caaccgggac gattgatatc cgtgtgcatg acacagacag caccaaaggg aaaaacggca
    10501 ttttccaaag tatcggtatc aaaaccgccc tttcactcca aaaataccaa atcgacaaac
    10561 cgggcaaaga aatcagaccg tgccgcctga aaaaacgccc acccgtccgt taaattaacc
    10621 tatccctgtt tcagacggcc tgaagcaggg atttttatat caaaataaaa tgagaaaggg
    10681 agcaataacc cttaggtagc tcttgttatt ttccgatgca aaacaaagca gtcatatatt
    10741 taattccccc tacctctgcc aagccttcct caaatattcg acgcaatcgg tcagcgagta
    10801 gaacgggaca ttgccgtggt cggcattcgg atattcccgg aaaaagacgg cggccccgtg
    10861 cctgtctaac tctgccgcca tttgttcggc ctgccctgcc atatcgcgtt cttccctgcg
    10921 tttacaatcg ctaccccgtt ctagcgcgcc gatgttgagg cagacatcga tgccgtttag
    10981 ccggttttca gacggcataa agtcgagtat ccgcctgttg tgccaccaaa tcgatgggga
    11041 tacgagccaa tgccgtctga aacggcggtg ggaaagcagg gaatacagtc cgaacagtgc
    11101 gccgaacgag tgtccgaata cggcggtttc attgcggttg agggtgtagc ggctttctaa
    11161 aaaggcggtc agctcgctgt cgataaaggc ggcgaagcgg tctgcctgtc cgaactgctg
    11221 ccgttcgtct gctgtggcgt tgtctccaag cggcggcgtg tagtcggcgg cacgttgtgc
    11281 caaatcgcgc acactgcctg tcgtgtagcc gataccgaca atcaggcagg gggcgttgct
    11341 tcgggtaacg gggttgggca tcagcgactg catgatgttg aaaagtgcgg ggaaaaaggc
    11401 ttcgccgtcc aaaacgaaga ggacgggata gccttcagac ggcatttcgc cgagtgtcgc
    11461 cgtctgaatc cgatagattc gccccgtgca ggtggatttg atttcggttt caaaggcttg
    11521 gggcagtatg gcaggttgga atgtgtcggt cggtatgggt ttcatgatgt tcggcttgtg
    11581 ggtcagactg gcggcaagcg gtaaaaccga accggcaaag cggtctgcct gccggttcgg
    11641 tctgttttcc ttcgcaatgc catactccag ttgtgagagc atagggtatg cagcgcagct
    11701 tgttgtggtt tgatgatgct gcggctgccg tttcagacgg catattggtc tttaaaaact
    11761 gtaacgcagg tttgccgtca ggctgcgctc cgaaccggga atgttaaagg tgttcgcgcc
    11821 gccgacgcgg gcgtagtaat ggcggttgaa gatgttgtcg gcgttgattt gcagcttcag
    11881 cttgggcgtg aagcggtatg ccgccatcgc atcgaacgtg gcataaccgc ctgcatgcat
    11941 ccctgcagat gaagtaatgc cgctcatcgc gttcacgccg ccgccgatgg tcagctcggg
    12001 cgtaacttgg taagtcgtcc acaggtttgc gctgtgtttg ggcatcaata ggaagatgcc
    12061 gtcgtcgcgt gaattggagg cggttttgat ttggctgtgc agatagctgt aacctgcatg
    12121 gatttgccat ttcggtgtaa ccgcgccgct gatttcggtc tcaacgcctt ccatcacgcg
    12181 tttgcccaat gcggcgtaac gggttttttt gttgttcggg ttcaacggtg cggcggcgtt
    12241 tttgtctttc atgcggtaga acgaaactcg ggcattgagg cggtcgtcca tgtagctgcc
    12301 tttgtaaccg acttcaaact ggttgccttg gcgcggtttg agcagcttgc cgtcggcatc
    12361 gaggttggtt tgcggtgtgt agagctggga caggctcagg tagaggctgt tgttgtcgtt
    12421 caagtcgtaa accgcgcctg cgtagccggt gaacttggtt ttgctggctt tgtgcagggt
    12481 tttgccttcg cccaactcga ttttatagtg tcccaaacgt ccgccgccga taagcgacag
    12541 cccgtcggca ggatggaagg tggatttgcc gtaaatgccg aattcgtcga ggttttcggt
    12601 agcgacggta tggctgtaac cgcgcacgcc tttgcgcgcg ttggcaatca aatcaacctg
    12661 cggtatgctg cggaactcgt ttaaagccag gccgccgcgt gcatataaag tagtacggcc
    12721 ttgttcgttg gtgctgcgga agcggttgta atcggcgccg atgacaaatt cgttggccgt
    12781 attgcccaag cggaaaggcc tgctgtagct ggcgtcaaac gcgagggctt tttgtttgat
    12841 ttctgtaccc aaacccaccg cgcaggcttt gtcgtcagcc gtattgcagc ccgggcggcc
    12901 tgccagggtt ttcatgccca gcttgctgcc ggcaaaggca tagttggagt cggcatcgcg
    12961 gtcggaatag cgcataccga ctttgccgta gccgccgttg ccgaagtaat gtttcaaatc
    13021 ggcaaacacg tcgtggctgt tcattttaaa tttgttccaa tccgcgccga caaatacgtg
    13081 ttgcggcagg gacggtaatt tgttattggc atcggctggc aagccgttgt acggcgcgag
    13141 gtggcgttgc tggtaaagat agcccgcgcc caaaaccgta tcggggttga tgtcccaatc
    13201 cgccgccgcg tagaaggttt cgtgccggtt gtttttctcg gcgggacgcg gagacgcgcc
    13261 gacggtctgc gccatcacgc ggccgcgcac gctgccgtct gaattgaggc tgcccgatac
    13321 gtccgcctcg gctttatatt gtttgtgcgt accgaaccct gccgcagcat gaccttggaa
    13381 cgctttggcc gggcgtttgc acaccagatt tacgatgccg cccatctcgc cgctgctgtc
    13441 gaacaagccg ctcggcccgc gcatcacttc cacacggtcg aaggcaaaca gattgggcag
    13501 cgtgccgttg atactctgca tctgcgcggg caggccgtcg atgttgtatt cgctgtattc
    13561 gtaaccgcgc gcgtaaaccg aagagcgtcc gtcgtcgttg ctcaacacgc gcaggccggg
    13621 cgttttgcgt gccaactggt caaacgtatc aacattgcgg tctttgacct gttgattggt
    13681 gatgatgctg acagattgcg gaatttcgcg caaagaagcg gggatttttg tgccgacggt
    13741 ggcggcaaac gagctgtaat cgccgttttt ctcggtggca atcgcgttgt acgaacgctg
    13801 acccttaata tggacggttt ccaaatccgc cgtttgtgcg gcaaaaaccg aagacgaaag
    13861 tgctgccaaa accgtggcgg cggtcatatt gatgcggaaa actgacataa actgtcccat
    13921 tcacataaat gataatggtt ctattttaat aaagcacaac gcggcttgtt cggaaaaaca
    13981 tatcgcgcag ccgacaaatt ttgtcgaaaa tgcgacacgt ctgcgttttc cgcataaaat
    14041 ctgacttttc accgtaaaca acctgccatg accacgccca aactcatcat cttcgactgg
    14101 gacggcacgc ttgccgatac gacccaaccc atcatcgaca ccatgcgccg cagcttcgcc
    14161 gaatgcggtt ttccgccgcc cgaagcggaa cgcgtccgca gcctgattgg ctacagcctg
    14221 cccgaaatca tccgcgacct gctcgaaatg ccgtctgaaa ccgccgttgc cgacatcgcg
    14281 cgcacttatt ccgcgcatta cctcaatccc aacaaccgca atatgacgct ctttcccgat
    14341 gccctgccct gtctggacaa gctcaaagca caaggcttcc ggcttgccgt cgccacgggc
    14401 aaagggcggg cgggtttgga caacgccatc agccaaaccg ccaccggcgg ctattggctc
    14461 gccaccgcct gcgcggggga atatccctcc aaaccctcgc ccgaaatggt attcggaatc
    14521 tgcggcgaac tggggttcga cccgaaagag gcattggtcg tcggcgatac ggcgcatgac
    14581 ctgcatatgg cggcaaacgc aggcgcggcg gcagtcggcg tggccaccgg cgcacattcg
    14641 cgcgaacagc tccttagcgc accgcatctc gccgtattgg acggtttgtc cgaactgccc
    14701 ggttttcttg cacgacatta cgcctgattg gtttccgcat ccggcacgcg gcaaaaatgc
    14761 cgtctgaagc ctgttcagac ggcatttgtg ttgcccaaac attcaacgct tgctcaacgt
    14821 ttgcacaaat cgggtttggt ttcgccctcg cggcgcagct ctttgggcag gacgaacaca
    14881 atgctttctt ccgcaccctc gccttcgcgt acggtttcgt gcccccatcc gcgtatggtt
    14941 gccagtactt cccgcaccaa cacttcgggc gcggacgcgc ctgccgttac gccgactttg
    15001 tttttgccct caaaccatgc gcgttgcagg tagcctgcat tatccaccat atacgcatcg
    15061 attccgcgcg atgccgccac ttcgcgcaag cggttgctgt tggacgaatt gggcgaaccg
    15121 accacaatca cgatgtcgca ctgttccgcc agctctttga cggcggtttg ccggttggtc
    15181 gtcgcatagc agatgtcttc cttgtgcgga ttgcggatat tggggaaaca cgcgttcagc
    15241 gcggcgatga tgtctttggt ttcatcgacc gagagcgtgg tttggctgac ataggcgagt
    15301 ttgtcggggt ttctgacttc gagttttgcc acatctccga ccgtttcgac caaaagcatt
    15361 ttgcccggcg caagctgccc catcgttcct tcaacctcga cgtgcccctt atgcccgatc
    15421 atgatgattt cacagtcttg ggcatccagt cgggcgactt ccttatgcac tttcgtcacc
    15481 agcgggcaag tcgcatcaaa cacgcggaaa ccgcgctccg ccgcttcttg ccgcaccgcc
    15541 ttcgatacgc cgtgtgccga ataaaccagt gtcgcgcccg gcggcacttc cgccaagtct
    15601 tcaataaaca ccgcaccttt ttcacgcagg ttgtccacga cgaatttgtt gtgaacgact
    15661 tcgtggcgca cataaatcgg cgcgccgaac tcttccaaag cacgttcgac aatactgatt
    15721 gcccgatcca caccagcgca gaagccgcgc ggattggcaa ggatgatggt tttctcgttc
    15781 ataagcccgg tatttcgttt tcagacggca tcaatatttt tcttcttggg ttttacggtg
    15841 gacgatgttg tccaacaccg ccaacaccgc accgacgcag ataaagctgt cggcaatatt
    15901 aaaggcggga taaaaccaat tttgccaata aaacaataag aaatcgacga catgaccgtg
    15961 tatcaggcgg tcgatgacat tgcctaacgc accgccgata atcattgccg cacccgtttt
    16021 gccgagggtt gcaaactcat cgcgcaagat ggcgcgtacc aaatacgcgc tcaccgccac
    16081 cgccagcacc aaaaaaaagt atttttgcca gccgccctga tcggcaagga agctgaacgc
    16141 cgcacccggg ttgtacacca gcgtcagatc gaaaaaggaa ggaatgacat tgacgcgttc
    16201 ccgatactga aacgacgaca gcaccgccca cttcgaccac tggtccagca cgatggcggc
    16261 aagtgccaat acccaatagc gcgttttact tgaaacagat gaagacatat ttttcaacag
    16321 ccggtaaaag agtaccattt tacccgaaaa ccccctttcc tgtacccgaa acggcaaatg
    16381 ccgtaatctt aaaacccgtc attcccgaca acaccgtaat ctcgaaaccc gtcattcccg
    16441 cgtaggcggg aatccagacc tgtccgcaca gaaacttatc ggataaaaac agttgcccaa
    16501 accccgcgtt ctatagtgga ttaaattcaa accagtacgg c
//
LOCUS       DAWEWP010000034        16473 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWEWP010000034 DAWEWP010000000
VERSION     DAWEWP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16473
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.034"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            241..1305
                     /locus_tag="WGN53_000790"
     CDS             241..1305
                     /locus_tag="WGN53_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ0139805.1"
                     /translation="MKAARFYDKGDIRIKDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGSGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKEGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1446..1769
                     /locus_tag="WGN53_000791"
     CDS             1446..1769
                     /locus_tag="WGN53_000791"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ0139806.1"
                     /translation="MGDSVLSTIQQTIDRRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMSSKDKDPSHLVYEVADLWFHTMILLAHHNLKAEDVLDELARRQGLSGLVEKA
                     ARTES"
     gene            1842..2165
                     /locus_tag="WGN53_000792"
     CDS             1842..2165
                     /locus_tag="WGN53_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ0139807.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2212..2415
                     /gene="tatA"
                     /locus_tag="WGN53_000793"
     CDS             2212..2415
                     /gene="tatA"
                     /locus_tag="WGN53_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ0139808.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2419..3105
                     /gene="tatB"
                     /locus_tag="WGN53_000794"
     CDS             2419..3105
                     /gene="tatB"
                     /locus_tag="WGN53_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ0139809.1"
                     /translation="MFDFGLGELVFIGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLSDGISDVMPSERSDTSAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRGLRPKHR
                     AKPKLRVRKS"
     gene            3119..3889
                     /gene="tatC"
                     /locus_tag="WGN53_000795"
     CDS             3119..3889
                     /gene="tatC"
                     /locus_tag="WGN53_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ0139810.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3942..4550
                     /gene="maf"
                     /locus_tag="WGN53_000796"
     CDS             3942..4550
                     /gene="maf"
                     /locus_tag="WGN53_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ0139811.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPANIDETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            4559..4912
                     /locus_tag="WGN53_000797"
     CDS             4559..4912
                     /locus_tag="WGN53_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ0139812.1"
                     /translation="MNRQTAYFLASFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4902..6716
                     /locus_tag="WGN53_000798"
     CDS             4902..6716
                     /locus_tag="WGN53_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ0139813.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALRLQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACKSLVAHYGQERIVTLLAWSLLIGSLLQSCIVVIQFAGWEDTPM
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPAPDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6906..7583
                     /locus_tag="WGN53_000799"
     CDS             6906..7583
                     /locus_tag="WGN53_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0139814.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7596..9002
                     /locus_tag="WGN53_000800"
     CDS             7596..9002
                     /locus_tag="WGN53_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0139815.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9120..11546
                     /locus_tag="WGN53_000801"
     CDS             9120..11546
                     /locus_tag="WGN53_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ0139816.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EENQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQSAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHSLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11591..11836
                     /gene="rpsP"
                     /locus_tag="WGN53_000802"
     CDS             11591..11836
                     /gene="rpsP"
                     /locus_tag="WGN53_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ0139817.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11852..12361
                     /gene="rimM"
                     /locus_tag="WGN53_000803"
     CDS             11852..12361
                     /gene="rimM"
                     /locus_tag="WGN53_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ0139818.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTVSVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12361..13110
                     /gene="trmD"
                     /locus_tag="WGN53_000804"
     CDS             12361..13110
                     /gene="trmD"
                     /locus_tag="WGN53_000804"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ0139819.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRILNKILQEQREIQS"
     gene            13126..13491
                     /gene="rplS"
                     /locus_tag="WGN53_000805"
     CDS             13126..13491
                     /gene="rplS"
                     /locus_tag="WGN53_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ0139820.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13813..14538
                     /locus_tag="WGN53_000806"
     CDS             13813..14538
                     /locus_tag="WGN53_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ0139821.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14562..15437
                     /locus_tag="WGN53_000807"
     CDS             14562..15437
                     /locus_tag="WGN53_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ0139822.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALVSVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15514..16428
                     /locus_tag="WGN53_000808"
     CDS             15514..16428
                     /locus_tag="WGN53_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ0139823.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLILLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4284 a         5289 c         3622 g         3278 t
ORIGIN      
        1 ccgtactggt ttttgttaat ccaccataaa tgccatctga agccgattcg gttttcagac
       61 agcatcatta tttgacctga cgcaaacaga aagccttccg cgtgcttaaa cacatctctt
      121 gtagtaaaat atctaaacag attcaactgg aaacggtcag gcttcttcac gccattccca
      181 tttcaaccgc cgcctgccag attcagacgg tattcctact tgaaaacaag gagcgttaca
      241 atgaaagcag cacgttttta cgacaaaggc gacatccgca tcaaagacat ccccgaaccg
      301 accgtcgccc ccggcaccgt cggcatcaat gttgcctggt gcggcatctg cggtaccgac
      361 ctgcacgaat tcatggaagg cccgattttc attccgcctt gcggtcatcc gcacccgatt
      421 tccggcgagt ccgcacccgt cacgatggga cacgagttct ccggcgtggt ctatgccgtc
      481 ggcgaaggcg tggacgacat caaagtcggt caacacgtcg tggtcgagcc ttacatcgta
      541 cacgacgacg taccgaccgg ctcgggcagc aactaccacc tctccaaaga catgaacttt
      601 atcggcttgg gcggctgcgg cggcggcctg tccgaaaaaa tcgccgtcaa acgccgttgg
      661 gtgcatccga tttccgacaa aatcccgttg gatcaagccg ctttgatcga accgctgtct
      721 gtcggacacc acgcctatgt acgcagcggc gcgaaagaag gcgacgtcgc attggtcggc
      781 ggtgcaggtc cgatcggttt gctgttggct gccgtgttga aagccaaagg catcaaagtc
      841 atcatcaccg agttgagcaa agcacgcaaa gacaaagcgc gcgaatccgg cgttgccgac
      901 tacatcctcg acccgtccga agtcgatgtt gtcgaagagg tgaaaaaact gaccaacggc
      961 gaaggcgtgg acgtggcatt cgaatgtacc agcgtgaaca aagtgttgga tactttggtc
     1021 gaagcctgca aacctgccgc caatttggtt atcgtatcca tctggagcca ccctgcaacc
     1081 gtcaacgtcc acagcgtcgt gatgaaagag ctggatgtgc gcggcacgat tgcctactgt
     1141 aacgaccacg ccgaaaccat caaactggtc gaagaaggca aaatcaacct cgaacccttc
     1201 atcacccagc gcatcaagct ggacgaactg gtttccgaag gcttcgagcg tctgatccac
     1261 aacaacgaat ccgccgttaa aattattgtg aatccaaacc tataagcaaa ttacatttgc
     1321 aacagcacaa atgccgtctg aaacgctcag acggcatttt ccaacgaaac gcaacaggcg
     1381 tattgcccga gagcagccgt ttaacctatc attccgaaca aacagaaaca aacagaagga
     1441 aaatcatggg agattccgta ctatccacca tccaacaaac catcgaccgg cgcaaatccg
     1501 ccgatccgtc cgaatcctac gtcgcacaac tcctgcacaa gggcgaggac aaaatcctaa
     1561 aaaaagtgat tgaagaggcg ggcgaagtat taatgtcatc caaagacaaa gacccgtccc
     1621 acctggttta cgaagtcgcc gacctttggt ttcacacgat gatcctgctg gcgcaccaca
     1681 acctgaaggc ggaagacgta ttggacgaac ttgcacgccg tcaggggctg tcggggctgg
     1741 tcgaaaaagc cgcccgcaca gaatcttaaa tttatattac aatctcatcc tttccacgtt
     1801 taatccgtct gggtgcttct tcagacggca acggagccgt tatggacaac tgtattttct
     1861 gcaaaatcgc cgccaaagac attccggcgc aaaccgtcta tgaagacggc gaaatggttt
     1921 gtttcaaaga catcaacccc gctgctccgg ttcatctgct gctgattccc aaagtccatt
     1981 tcgattcgtt ggcacacgcc gcgcccgaac atcagcccct tttgggaaaa atgatgctga
     2041 aagttcccga aatcgccaaa gcggcaggac tggcagacgg cttcaaaacc ctgatcaaca
     2101 ccggaaaagg cggcggacaa gaggtcttcc acctgcatat acacatcatg ggcacacccg
     2161 tataaaccgt tatttcacaa tcaaccccta atacttactt aaggatacat catgggcagt
     2221 ttttctctga cgcactggat tatcgtactg attatcgtcg ttttgatatt cggcaccaaa
     2281 aaactgcgca acgtcggcaa agacctcggc ggtgcggttc atgacttcaa acaggggctg
     2341 aacgaaggta cagacggcaa agaagcccaa aaagacgatg taatcgaaca caaaaaagac
     2401 gaagacaaag cgtaatttat gtttgatttc ggtttgggcg agctggtttt tatcggcatt
     2461 atcgccctga ttgtcctcgg ccccgaacgc ctgcccgagg ccgcccgcac cgccggacgg
     2521 ctcatcggca ggctgcaacg ctttgtcggc agcgtcaaac aggaatttga cacgcaaatc
     2581 gaactggaag aactaaggaa ggcaaagcag gaatttgaag ctgccgctgc tcaggttcga
     2641 gacagcctca aagaaaccgg tacggatatg gagggtaatc tgcacgacat ttccgacggt
     2701 ctgaagcctt gggaaaaact gcccgaacag cgcacgcctg ctgatttcgg tgtcgatgaa
     2761 aacggcaatc cctttcccga tgcggcaaac accctatcag acggcatttc cgacgttatg
     2821 ccgtccgaac gttccgatac ttccgccgaa acccttgggg acagcgggca aaccggcagt
     2881 acagccgaac ccgcggaaac cgaccaagac cgcgcatggc gggaatacct gactgcttct
     2941 gccgccgcac ccgtcgtaca gaccgtcgaa gtcagctata tcgatactgc tgttgaaacg
     3001 cctgttccgc acaccacttc cctgcgcaaa caggcaataa gccgcaaacg cggtttgcgt
     3061 ccgaaacacc gcgccaaacc taaattgcgc gtccgtaaat cataaagaga gcaatccggt
     3121 gtccgaaaca caaaacgaac aacccgtcca accgcttgtc gagcatctca tcgagctgcg
     3181 ccgccgcctg atgtggacgg ttgtcggcat cttagtctgc tttttcggcc taatgccgtt
     3241 tgcccaacaa ctctatactt ttatcgccga cccgctgatg gcaaacctgc ccaaagacac
     3301 cagcatgatt gccaccgatg tcatcgcacc gtttttcgtg ccggtcaaag ttaccctgat
     3361 ggcggcattt ttaatttcgc tgccgcatac gctctaccaa atctgggcat ttgtcgcgcc
     3421 cgcactctac caaaacgaaa aacgcctgat tacgccgctc gtcctctcca gcgtcagcct
     3481 gtttttcatc ggcatggcat ttgcctactt tttggttttc cccgtcattt tcaaattcct
     3541 tgccagcgtt acccctgtcg gtgtcaatat ggcgacagac atcgacaaat acctctcctt
     3601 catcttgggg atgtttgtcg cattcggtac aacgtttgaa gtccccattg tcgttatcct
     3661 gttaaccaaa attggtgtgg taacaaccga acagctcaaa cgcgcccgcc cctatgtgat
     3721 tgtcggcgcg tttgtcattg ccgccatcat cacgccgccc gatgtgattt cacaaaccct
     3781 gcttgccatt ccgctgattc tcttatacga agcaggtatt tggttcggac gctttttcac
     3841 gccacgttca gaacaggatg gcgacataca gccgcctgca acaacctgac actatgccgt
     3901 ccgaacctcc gcctcatacc gcaacagatt aaggaatacc tttgaatacc ctctatttag
     3961 gttcaaacag cccgcgccga atggaaatcc tgacacagtt gggctatcag gtcgtcaagc
     4021 tgcctgccaa catcgacgaa acggtcagac agaacgaaga ccctgcccgt tacgttcaaa
     4081 ggatggcaga agaaaaaaac cgaaccgccc tgaccctctt ttgcgaaacc aacggcacaa
     4141 tgcccgattt ccccctgatt accgccgaca cctgcgtcgt ttcagacggc attatattag
     4201 gcaaaccccg ctcccaagcc gaagcaatcg aatttttaaa ccgattgtcc ggcaaacaac
     4261 ataccgtcct gactgctgtc tgcattcatt atcgcggcaa aacgtcaagc cgcgtccaaa
     4321 ccaaccgcgt tgttttcaag cccctgagtt cggaagaaat ttccgcctat gtgcaaagcg
     4381 gcgaaccgat ggacaaagcc ggcgcctacg ccgtacaagg cataggcggc atctttatcc
     4441 aatctatcga aggcagcttc agcggcatta tgggactgcc cgtttatgaa accgtttcga
     4501 tgttgcagga tttgggatac cgctccccct tgtccgccct taaaccgtaa agaccgccgt
     4561 gaacagacaa accgcttact tccttgcctc cttcagcctg atcgcactga taatcctgtc
     4621 cctttcttgg gaactgtgga ttgcaccgtt acgcccgggc ggctcgtggc tcgcgctcaa
     4681 agccctcccc ctctgtctgc cgctttcagg catcttgaaa aagaaaatct atacctacca
     4741 atacagctcc atgctggttc tgatttattt tgccgaagcc gtcatgcgcc tgttcgacgc
     4801 ctatccggca gaacaaattt gcgccgcgct ttccgcagta ttcagcatca tcttcttcat
     4861 atcctgcctg tcgttcgtca aacaatacaa ggaaacaaac aatgcccgct gaaacgaccg
     4921 tatccggcgc gcaccccgcc gccaaactgc cgatttacat cctgccctgc ttcctttgga
     4981 taggcatcgt cccctttacc tttgcgctca ggctgcaacc gtcgcccgac ttttaccacg
     5041 atgccgccgc cgcagccggc ctgattgtcc tgttgttcct cacggcagga aaaaagctgt
     5101 ttgatgtcaa aatcccacct atcagcttcc ttctgtttgc aatggcggcg ttttggtatc
     5161 ttcaggcacg cctgatgaac ctgatttacc ccggtatgaa cgacatcgtc tcttggattt
     5221 tcatcttgct cgccgtcagc gcgtgggcct gcaagagttt ggtcgcacac tacggacaag
     5281 aacgcatcgt taccctgctt gcctggtcgc tgcttatcgg ctccctgctt caatcctgca
     5341 tcgtcgtcat ccagtttgcc ggctgggaag acacccccat gcttcaaaac attattgttt
     5401 acagcgggca aggcgtaatc ggacacatcg gacagcgcaa caacctcgga cactacctga
     5461 tgtggggcat actcgccgcc gcctacctca acggacaacg aaaaatcccg cccgccctcg
     5521 gcgcaatctg cctgattatg cagaccgccg ttttaggttt ggtcaattcg cgcaccatct
     5581 tgacctacat agccgccatc gccctcatcc ttcccttctg gtatttccgt tcggacaaat
     5641 ccaacaggcg gacgatgctc ggcatagccg cagccgtatt ccttaccgcg ctgttccaat
     5701 tttccatgaa caccattctg gaaaccttta ccggcatccg ctacgaaact gccgtcgaac
     5761 gcgttgccaa cggcggtttc acagacttgc cgcgccaaat cgaatggaat aaagcccttg
     5821 ccgccttcca gtccgccccg atattcgggc acggctggaa cagttttgcc caacaaacct
     5881 tcctcatcaa tgccgaacag cacaacatat acgacaacct cctcagcaac ttgttcaccc
     5941 attcccacaa catcgtcctc caactccttg cagagatggg aatcagcggc acgcttctgg
     6001 ttgccgcaac cctgctgacg ggcattgccg ggctgctgaa acgccccctg acccccgcat
     6061 cgcttttcct aatctgcacg cttgccgtca gtatgtgcca cagtatgctc gaatatcctt
     6121 tgtggtatgt ctatttcctc atccctttcg gactgatgct cttcctgtcc cccgcagagg
     6181 cttcagacgg catcgccttc aaaaaagccg ccaatctcgg catactgacc gcctccgccg
     6241 ccatattcgc aggattgctg cacttggact ggacatacac ccggctggtt aacgcctttt
     6301 cccccgcccc tgacgacagt gccaaaaccc tcaaccggaa aatcaacgag ttgcgctata
     6361 tttccgcaaa cagtccgatg ctgtcctttt atgccgactt ctccctcgta aacttcgccc
     6421 tgccggaata ccccgaaacc cagacttggg cggaagaagc aaccctcaaa tcactaaaat
     6481 accgccccca ctccgccacc taccgcatcg ccctctacct gatgcggcaa ggcaaagttg
     6541 cagaagcaaa acaatggatg cgggcgacac agtcctatta cccctacctg atgccccgat
     6601 acgccgacga aatccgcaaa ctgcccgtat gggcaccgct gctacccgaa ctgctcaaag
     6661 actgcaaagc cttcgccgcc gcgcccggtc atccggaagc aaaaccctgc aaatgacccg
     6721 cgccggcgga tacggatacc gcccgaaatg taaagctcca tgcaagacat tgcaaaaaac
     6781 agcaaaccgg tagggaaata cgctatcaaa aaacattgca gccgtgttaa gataaaccgt
     6841 caaacaatct tttcacgccc cgcccgaaac agggtcgggg cataccctta cgaaaaggaa
     6901 acaccatgag ccgcgtatta ctcgtagatg acgatgccct gctgaccgaa ctgctgaccg
     6961 aatacctgag cgccgaaggt ctgaacgtcc gcagcgttcc cgacggggaa gcaggcgtac
     7021 aggaaatcct gagcgggcag tacgatgtag tcgtattgga ttccatgatg cccaaaatga
     7081 acggcttgga tgtcttgaaa aacgtacgcg cccgaagcac cgtccccatc atcatgctga
     7141 ccgccaaagg cgacgacatc gaccgaatca tcggcttgga aatgggcgcg gacgactatg
     7201 tccccaaacc ctgcacacca cgcgaactct tggcacgcat caatgccatc ctgcgccgcg
     7261 cacaacacag cggcgaacag aacaacgcac ccaacagcat ctccgtcagc gatgtcgtcc
     7321 tgtaccccgc caaacgccag gcatccgtca aagacatgcc gctcgaactg accagcaccg
     7381 aattcaacct gctcgaagtc ctgatgcgcc atgccggaca ggtagtcagc aaagaaaccc
     7441 tgtccgtcga agcactcgac cgcaagctgg caaaattcga ccgcagtatc gacgtacaca
     7501 tctccagcat ccgccacaag ttgggcgatg cctctctgat tcaaaccgta cgcggcttgg
     7561 gctacctgtt tgtcaaaaac tgaagcaacg ggcaaatgaa actgttccaa cgcattttcg
     7621 ccacattttg cgcagttatc gtctgtgcaa tctttgtggc gagtttttct ttttggctgg
     7681 tgcagaacac ccttgccgaa aaccagttca accaacgccg caccatcgaa accactttga
     7741 tgggcagcat catttccgca ttccgggcac gcggggacgc gggcgcgcgc gaaatcctga
     7801 cggaatggaa agacagcccc gtctcatcgg gcgtgtacgt tatacagggc gacgagaaaa
     7861 aagatatcct gaaccggtat atcgacagct acaccatcga acgcgcccgg cttttcgccg
     7921 ccggacaccc gcattccaac ctcgtccata tcgaatacga ccgcttcggc gaagaatacc
     7981 tgttcttcac caaagactgg gacaaactcc aagcccgccg cctgcccagc cccctgttga
     8041 tccccggcct gccgctcgcc ccgatttggc acgaactcat catattgtcc ttcatcatcg
     8101 tcgtcggact gctgatggcg tacatcctcg ccggcaatat cgccaaaccc atcagaatct
     8161 taggcaacgg catggacagg gtggcaaacg gagaacttga aacccgtatc tcccaacagg
     8221 tcgacgaccg cgacgacgaa ttgtcccatc ttgccatcca attcgacaaa atggtggaaa
     8281 aactcgaaaa actcgttgcc aaagaacgcc acctgctcca ccacgtttcc cacgaaatgc
     8341 gctccccgct tgcccgtatg caggcgattg tcggactgat tcaggcgcag ccccaaaaac
     8401 aggaacaata tctcaaacgg ctggaaggcg aactgacccg catggatacg ctggccgggg
     8461 aactgttaac cctgtcccgt ctcgaaactt ccaatatggc tttggaaaaa gaaagcctga
     8521 aactcctgcc cttcctgggc aacctggtag aagacaatca aagcattgcc caaaaaaacg
     8581 gacaaacggt taccctgtct gccgacggaa aaatccccga aaacacaacc atccttgcca
     8641 acgaaagcta cctgtaccgc gccttcgaca acgtcatccg caacgccgtc aactacagtc
     8701 ccgaaggcag caccatcctg atcaacatcg gacaagacca caaacactgg ataatcgacg
     8761 ttaccgacaa cggccccggc gtggacgaaa tgcagctccc gcacatcttc accgctttct
     8821 accgtgcaga ctccagtgcc aacaaacccg gaacaggact ggggcttgca ttgacccaac
     8881 atattattga acagcactgc ggcaaaatca tcgccgaaaa catcaaaccg aacgggctgc
     8941 ggatgcgctt catcctaccc aagaaaaaaa ccggttccaa aacagaaaaa agtgcgaact
     9001 gaccataatg ccgtctgaac cggcttcaga cggcattgca caaacagtta ccccaaaaca
     9061 aacacacagc gggtgcatct ccaaaaatca cgcacatacc gtccgcaaag gaatatatca
     9121 tgtcggcaca aaccgatccc ggctacttct tcatgcccaa ccacattatc ctgataggcg
     9181 cgagcgaacg tccgtacagc ctgggcgaac gcgtactcag caacctgctg agtacgccct
     9241 ttcaaggaaa aatcaccccc gtaaacccgc gccaccacac catagccggg cttcccgcct
     9301 acaccagcct caacaaaatc cccggcaatg cagacctgat tattgccgtt accctacccg
     9361 acagttacga caccctcttc aaaacctgcc gcaaaaagca gctccgacac atcatcctca
     9421 tacaggactg ggacagcctg tctgccgcag aactgcacac cgccgaaact gccatccgca
     9481 aacaccacgg caacggactc aacatcaccg cctgcaccac cgcaggcatc caactgccct
     9541 cactcggact taacatcagt acccaagacg gatacgccgc aggccatacc gccatactga
     9601 ccggcaatgc cgccgtcagc cgccaaatcg acaacatcct gaacaaactc cgtcaaggca
     9661 catcccgcca catcagcctg catcccggca tcagccccat cacatccgcc gattggctca
     9721 accgcttcgg acacagcctg cacaccaaaa ccgccgtcct acaccacaac cctgaagaga
     9781 atcagcgcaa actgttcagc gcaatccgcc aatttacccg ccatacgccg ctgattctcc
     9841 acatcacctg ccgcacgaca gaaaccgacc gtgccgtact gcactgcctc gcccgacact
     9901 gcaacttcct catcagtttc aacgccgacg acctcgaagc cgcactgcgc gcccaactgt
     9961 ccgaccttcc acccctgtcc cgactcgaca tcctgtccga cacgcctgcc gaatggctga
    10021 acgcgcacgc gccaaaaacc ctcgccctcc actttcccaa ccttccccac cacatccgca
    10081 acggacacct gaccggcaca cccacaccct caatctgcca cgacatcgcc tcacgtcagc
    10141 ttgcccgccc cgacacccaa gccgtcctga ccatcctcag cccctccgga cacgaggatt
    10201 acaaaaaaac agcacgcgcc cttatccgcc tgtccgaaca gagcgccaaa cccctgctcg
    10261 tcagcagccc cttttcagac ggcataacac atttcgacac ccccactcag gcaatccgca
    10321 ccctttccta ccgcaacacc gccgccgccc tgaaacaggc acagctcgac attgcaccgc
    10381 cgcagccatg ccgtctgaaa acaccgcaac cccaaaacat caaaaaagcc ctcgaagccg
    10441 ccgaccccgc cctaatcgcc gaagccctgc acctcccccc ctaccggcac accacccata
    10501 acgccgtcca attccaattc ggcagccact ccctctatgg cgacatcctg accgcacgtt
    10561 gcaacggaca aaccactgcc gtactcccgc cgtttaccac gctcgacagc cgccaccttg
    10621 cccgctttgc cgaactcgac ggcacacaaa ccctcgacca gttcctgcac acactgacca
    10681 tcattcccga ataccgccaa cacattctcg gcatcaccct caacctaaac ggcggacaat
    10741 acagcagcga tttcatctta aagacacccg aaacacacga cacgcccaaa cgcaagaaca
    10801 caggcaaagc cgcccaaacc ctcgaacatg ccgccgcaaa aatgcagagt gccgccgcat
    10861 acctgaaaca caaaaacccg acagccgccg aatttctccg ccacacaagc gaagccgccg
    10921 cagaactgct cggcagcaaa accgaaaccg gagcagccgt acccaacgta cttgccccct
    10981 atcccgcagc ataccccaaa acactgtccc taagaaacaa cacgaccgtt accattaccc
    11041 ccattttgcc cgaagacgca gaagccaaac agcagttcgt ccgcagcctc ggtcccgaag
    11101 cacggtacac acgcttcatg acccacacca acgaactgcc cgcagccacg ttggcacgcc
    11161 tgtgcaaccc cgattaccac tgtgaagccg catggacggc aaaggatgcc gacagcaaca
    11221 tcgtcgccgt cgtccgccac agccgcctga accgcaacga atgcgaattc ggcatcacac
    11281 tggcggaaca tatgcgcggc agcggactgg cacagaaaat gatggaactc atcatccaaa
    11341 ccgccgcaca gcaaggctac cggactatga gtgccgacat tctcaaaacc aataccccca
    11401 tgatcaaact tgctgaaaaa tcaggattta ccctcaagga atcggacacc gaaaaaaacc
    11461 tgtaccgcgc atatctgaac cttgcggcag acaaaacaac agaaaaaaca aataaaaact
    11521 tgcgcaccga ccacaaaata acctaaaatc ggcagtttcc atatatccga ttttctcaaa
    11581 aggactcaaa atggtagtta tccgtttggc acgcggcggc tcgaaacacc gccccttcta
    11641 caacgtcatc gttactgact cacgcagccg ccgcgacggc cgcttcatcg aacgcgtagg
    11701 cttctacaac cccgtagcca acgaaaaaca agagcgcgtc cgcctcaatg cagaccgcct
    11761 gaaccactgg attgcacaag gcgcgcaagt cagcgactcc gttgcaaaac tgattaaaga
    11821 acaaaaagcc gcctaattca catttgccgc catgacagac actcaaaacc gggtagccat
    11881 gggctacatc aaaggcgtat tcggcataaa aggctggttg aaaattgccg ccaacaccga
    11941 atattccgac agccttttgg actaccccga gtggcatttg gtcaaggacg gcaaaaccgt
    12001 cagcgttacc cttgaagccg gaaaagtcgt caacagcgaa ctccaagtca aattcgaagg
    12061 cataaacgac cgcgacttgg cattctcatt gcgcggttac accatcgaaa taccccgtga
    12121 agcattcgcc ccgacagaag aagacgaata ctactggaca gacttggtcg gcatgaccgt
    12181 cgtcaacaaa gaccataccg ttttaggcaa ggtaagcaac ctgatggaaa ccggcgcaaa
    12241 cgacgtattg atgattgacg gagaacacgg gcagattctg attccgttcg tttcccaata
    12301 tatcgaaacc gtcgataccg gcagcaagat tattactgcc gactggggtt tggactactg
    12361 atgcttatcc aggcagttac cattttcccc gaaatgttcg acagcattac ccgctacggc
    12421 gtaacgggac gcgcgaacag acagggaatc tggcagtttg aagcagtcaa tccccgaaag
    12481 tttgccgaca acagattggg ctacatcgac gaccgcccgt tcggcggcgg cccgggaatg
    12541 attatgatgg ctccgccgct tcatgcagca atagaacacg ccaaagcaca atcctcccaa
    12601 gctgcaaaag tcatctacct cagcccccaa gggaaaccgc tgacacacca aaaagcggta
    12661 gaactggcag aacttccgca tctgattctg ctgtgcggac ggtatgaggg catagacgaa
    12721 aggcttctgc aaagcagcgt cgatgaagaa atcagcatcg gagacttcgt tgtttccggc
    12781 ggagagcttc ccgccatgat gctgatggat gcggtattga ggctcgtacc cggcgtattg
    12841 ggcgatatgc agtctgccga acaagattcg ttctcaagcg gcattttgga ctgcccccac
    12901 tacaccaaac ccttagaatt tcaaggcatg gctgttccgg aagtattacg ttccggcaat
    12961 catggcttga tagcggaatg gcggttggaa caatcgctgc gccgcacctt ggagcgcaga
    13021 cccgatcttt tggaaaagcg cagtttaatc ccaaaggaat cccgcatctt gaataaaatc
    13081 ctacaagagc aacgggaaat ccaatcataa tttaggaaaa aaacaatgaa cctgattcaa
    13141 cagctcgagc aagaagaaat tgcccgcctg aacaaagaaa tccccgaatt cgcaccgggc
    13201 gacaccgtag tcgtatccgt acgcgtcgtg gaaggtaccc gcagccgtct gcaagcctac
    13261 gaaggcgttg tcattgcacg ccgcaaccgt ggtctgaaca gcaacttcat cgtccgtaaa
    13321 atttccagcg gcgaaggtgt tgaacgtact ttccaactgt actctccgac cgtcgaaaaa
    13381 atcgaagtca aacgccgtgg cgacgtacgc cgtgccaaac tgtactacct gcgcggtctg
    13441 accggcaaag ctgcacgcat caaagaaaaa ctgcctgcac gcaaaggttg attcaaaccc
    13501 gttttcccca atgccgtctg aaccttctga cggcatttct tattgccgca acatccgcaa
    13561 cggaacagca ccaagaagca caccccttcc ggcacatccc gaatccccgt ccgaaccgaa
    13621 caaaatttac agaccaaacg ttatacagta tcatatcagc catataataa taaccgcttt
    13681 accgccttcc ctttcaaaac cgcaaatcat catgacacct tcccttttac tatcaggatt
    13741 gaccttccgc ctcgtccttg ccctgattgc cgtatccctt ttatggggcg tttacttttg
    13801 ggcagtatcc gcatgagcat cattgtcgaa aacctgaccg tcagctaccg ccgccgacct
    13861 gccgtgcacc atgtggacat tacttttgaa gaacatagta tgtgggcggt tttcggtccc
    13921 aacggcgcag ggaaatccac ctttctcaaa tccttaatgg gattgcagcc tatcgatacg
    13981 ggcagcatcc ggctggacgg attgacccgt cagaacattg cctaccttcc ccagcagtcc
    14041 gacatcgacc gctcgcagcc catgaccgtt ttcgacttgg cggcaatggg gctatggtat
    14101 gaaatcggtt ttttcagagg aataaatgcc gttcaaaaac aacgcgttca cgaatctttg
    14161 gaacgcgtcg ggatgcggca gtttgccggc cgccaaatcg cccacctctc aaacggacaa
    14221 ttccagcgtg tcctttttgc ccgaatgctg gttcaaaacg ccaaattcct acttctagac
    14281 gaacctttca acgctgttga tgcacggacg acctacgaac ttctcgacgt attgcagaaa
    14341 tgccattgcg gcggacacgc catcatcgcc gtactgcacg attacgaaca agtccgtgcc
    14401 tactttccca ataccctgct gctcgcccgc gaaaaaattg cggcaggcgc aaccgagacc
    14461 attctgacag aaccctacct cgcccaagcc aacgccaaaa tgcagcaaca ggaaagcccc
    14521 gagtggtgcg cgtcataaat gccgtctgaa accgaaaaac catgaacctc tacgacctgc
    14581 tccttgcccc ctttgcagaa tttgacttta tgcgctacgc cctcgtatcc gtcttctgcc
    14641 tgtccctcag tgccgcaccc gtcggcgtat tcctcgtcat gcgccgtatg agcctgatag
    14701 gcgacgcatt gagccacgcc gtcctgcccg gtgccgccgt cggctacatg tttgccggct
    14761 taagcctgcc cgccatgggt ttgggcggcg tagccgcagg tatgctgatg gcactgcttg
    14821 ccggactcgt cagccgcttc accaccctga aagaagatgc caactttgcc gccttttatc
    14881 tcagcagcct cgccatcggt gtagtcctcg tcagcaaaaa cggcagcagc gtcgatctgc
    14941 tgcatctgct tttcggctct gtacttgccg tcgatattcc tgccctgcag ctcatcgccg
    15001 ccgtatccag cctcacgctc attacccttg ccgtcatcta ccgcccgctc gtactcgaaa
    15061 gcatcgaccc cctgtttctc aaatctgtcg gcggcaaagg cgggctttgg cacgtcctct
    15121 ttctcgtcct ggtcgtcatg aacctcgtat ccggctttca agccctcggc acactcatgt
    15181 ccgtcggact catgatgctg ccagccatta ccgcccgcct gtgggcgaag cacatgggcg
    15241 cactcatcct cctatccgtt ctgacagccc tgctgtgcgg cttgagcggg ctgctcattt
    15301 cctaccacat cgaaatccct tccggccccg ccatcatcct ctgttgcagc gtcctttatc
    15361 tcttttccgt catactcggc aaagaaggcg gcattctgac caaatggctc aaaaaccacc
    15421 gccaccacac cacctgaccc cgcaagccgc ttgaacaaac aggcggcttg ttttaccata
    15481 acagatgtta cgttatatca caggagaaca gttatgaaac acctcaaact cacccttatt
    15541 gccgcattgc tgaccactgc cgcaactgcc gccccccttc cggttgtaac cagtttcagc
    15601 attttaggcg acgttgccaa acaaatcggc ggagagcgcg tatccataca aagtttggtc
    15661 ggagccaacc aagatactca tgcctatcac atgaccagtg gcgacattaa aaaaatccgc
    15721 agtgcaaaac tcatcctgct caacggctta ggacttgaag ccgccgacat ccaacgtgcc
    15781 gtcaaacaga gcaaagtatc ctatgccgaa gcgaccaaag gcatccaacc cctcaaagcc
    15841 gaagaagaag gcggacacca tcatgaccac gaccatgacc acgaaggaca ccaccatgac
    15901 cacggcgaat ttgacccgca cgtctggaac gaccccgtcc tcatgtccgc ctatgcccaa
    15961 aacgtcgccg aagccctgat aaaggccgac cccgaaggca aagtttatta tcaacaacgc
    16021 ttgggcaact accaaatgca gctcaaaaaa ctgcacagcg acgcacaagc cgcatttaac
    16081 gccgtccctg ccgccaaacg caaagtcctg accgggcacg atgccttttc ctacatgggc
    16141 aaacgttacc atatcgaatt catcgccccg caaggtgtga gcagcgaagc cgagccttca
    16201 gccaaacaag tcgccgccat catccgacaa atcaaacgcg aaggcatcaa agccgtattc
    16261 accgaaaata tcaaagacac ccgcatggtt gaccgtatcg ccaaagaaac cggtgtcaac
    16321 gtcagcggca aactgtattc cgacgcactc ggcaacgcgc ccgcagacac ctacatcgga
    16381 atgtaccgcc acaacatcaa agccttgacc aacgcgatga agcaataaaa ccgtttgatt
    16441 ggtttgaatt tgaaaaggca aaaggccgtc tga
//
LOCUS       DAWEWP010000035        15528 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWEWP010000035 DAWEWP010000000
VERSION     DAWEWP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.035"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            6..81
                     /locus_tag="WGN53_000809"
     tRNA            6..81
                     /locus_tag="WGN53_000809"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="WGN53_000810"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="WGN53_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ0139824.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="WGN53_000811"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="WGN53_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ0139825.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="WGN53_000812"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="WGN53_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ0139826.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="WGN53_000813"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="WGN53_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ0139827.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2472..2972
                     /gene="rplJ"
                     /locus_tag="WGN53_000814"
     CDS             2472..2972
                     /gene="rplJ"
                     /locus_tag="WGN53_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ0139828.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3030..3401
                     /gene="rplL"
                     /locus_tag="WGN53_000815"
     CDS             3030..3401
                     /gene="rplL"
                     /locus_tag="WGN53_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ0139829.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3592..7770
                     /gene="rpoB"
                     /locus_tag="WGN53_000816"
     CDS             3592..7770
                     /gene="rpoB"
                     /locus_tag="WGN53_000816"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ0139830.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7927..12102
                     /gene="rpoC"
                     /locus_tag="WGN53_000817"
     CDS             7927..12102
                     /gene="rpoC"
                     /locus_tag="WGN53_000817"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ0139831.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKVALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRERILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            12360..12731
                     /gene="rpsL"
                     /locus_tag="WGN53_000818"
     CDS             12360..12731
                     /gene="rpsL"
                     /locus_tag="WGN53_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ0139832.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            12849..13319
                     /gene="rpsG"
                     /locus_tag="WGN53_000819"
     CDS             12849..13319
                     /gene="rpsG"
                     /locus_tag="WGN53_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ0139833.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            13338..15443
                     /gene="fusA"
                     /locus_tag="WGN53_000820"
     CDS             13338..15443
                     /gene="fusA"
                     /locus_tag="WGN53_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ0139834.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4144 a         3446 c         4011 g         3927 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgccggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaacgttaa agtggatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggaagggtt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgctgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcgt attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ctagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 cccccgtgtt tctggtttta ttggagggag ggctaataga cctacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gtcggtcaac aggttcgtgt aaatgaaggg ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgaa aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagtccgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggt aaagccaatc catctcctcc ggttggtcct gctttgggtc
     1201 agcgcggttt gaatattatg gaattttgta aggcatttaa tgctgcaacc caaggtatgg
     1261 agcctggctt accgattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac cccgccagct tctatcttgt tgaaaaaggc tgccggtttg caaaaaggta
     1381 gttctaatcc tctgaccaac aaagtgggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtc cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggatg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagctcttc gctcttctgt ggaagccaat aaattatatg caattgatga
     1621 agcaattgct ttggtaaaaa aagcagcgac tgctaaattt gacgagtctg ttgacgtatc
     1681 tttcaacttg ggcgttgatc cgcgtaaatc tgaccaagtt atccgtggtt cggtcgttct
     1741 gcctaaaggc accggtaaga taacccgtgt ggctgtattt actcaaggtg caaatgcaga
     1801 agctgctaaa gaagctggtg cagatatcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg acgttgttat tgcttctccc gatgcaatgc gtattgttgg
     1921 tcaattgggt actattttgg gtcctcgagg cttgatgcca aaccctaaag taggtactgt
     1981 tactcctaac gttgctgaag cagtaaaaaa tgcaaaagca ggtcaagtgc agtaccgtac
     2041 cgacaaggca ggtattgttc atgcaacgat tggtcgtgct tcattcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggatgc tatcgttaaa gccaagcctg ctgccgctaa
     2161 aggtcagtat ctgaaaaaag ttgctgtgtc tagcaccatg ggtttgggta ttcgcgttga
     2221 tacatcaagc gtaaataact aatcttaagg aattttcaag cagtttggtt ttctgggctg
     2281 cttgaatttg ggctacttaa aattaagtag atgtccaaga ccgtagggat cgtaagattt
     2341 aatcgtaact gccctacgca gacggtagtc ctgaaacaca ttgcaagatt gcttgtaaga
     2401 tgtcttttta ggttaccgcg ctggtgggat atcgttttgg tatcctgttt ataaacagtg
     2461 ggaggtagac cttgagtctc aatattgaaa ccaagaaagt ggcggtcgag gaaattagcg
     2521 cggcaattgc taatgctcaa accctcgtag tcgctgaata tcgcggtatc agtgtttcca
     2581 gtatgactga gcttcgtgcg aatgcacgta aagaaggcgt ttatttgcgc gttctgaaaa
     2641 atactttggc tcgtcgtgca gtgcaaggta cttcatttgc agaattggcc gatcaaatgg
     2701 ttggtccgtt ggtttacgct gcttctgaag atgctgttgc tgctgctaaa gtgttgcacc
     2761 aattcgcgaa aaaagatgac aaaattgtcg ttaaagccgg ttcttacaat ggcgaagtaa
     2821 tgaatgctgc tcaggttgct gagttggctt ctattccgag ccgcgaagag ctgttgtcca
     2881 aactgttgtt cgttatgcaa gctcctgtat cgggctttgc gcgcggtttg gctgctttgg
     2941 cagagaaaaa agccggcgaa gaagccgctt aatcgatttt gtttctgtta atcaattatt
     3001 ttttaataca atatttggag taaaatagca tggctattac taaagaagac attttggaag
     3061 cagttggttc tttgaccgta atggaattga acgacttggt taaagctttt gaagaaaaat
     3121 tcggtgtttc tgctgctgct gttgcagttg caggtcctgc tggtgccggt gctgccgatg
     3181 ctgaagaaaa aaccgaattt gatgtcgttt tggcttctgc cggcgatcaa aaagtcggcg
     3241 tgattaaagt tgtccgtgca attaccggtt tgggtctgaa agaagctaaa gacatcgttg
     3301 acggtgcacc taaaaccatt aaagagggtg tttctaaagc tgaagccgaa gacatccaaa
     3361 aacaactgga agaagccggc gctaaagtcg aaatcaaata atttgatgct tcttatgaag
     3421 gctggcagtt ttctgccagc cttattttgc ttcttaaaat aaacatcaag tattgtttac
     3481 atttatttgc atagttttta tcaaatcatt gcaaataaat gtaaacatca gattgatgcg
     3541 tgccgttgct ttcaaacggc ctattattga aaattacttt tcggagtgta tatgagctat
     3601 tcgtttaccg agaaaaaacg tatccgtaag agttttgcaa agcgggaaaa tgttttggaa
     3661 gttcctttct tgctagcaac ccaaattgat tcttatgcga agtttttgca gctggaaaat
     3721 gcttttgaca aacgtaccga tgacggtctg caggcagcat ttaattctat tttcccgatt
     3781 gtgagccata acggttatgc gcgattggag tttgtgcatt acacattggg cgagcctttg
     3841 ttcgatattc ccgaatgtca gttgcgcgga atcacttatg cagccccctt gcgcgcgcgt
     3901 atccgtttgg tgattttgga taaggaagca tctaaaccga cggtaaaaga agttcgtgaa
     3961 aacgaagtgt atatgggcga aattccgttg atgaccccga gcggttcttt tgtgattaac
     4021 ggcacagagc gtgtgattgt ctcccagttg caccgttcgc ccggcgtatt cttcgagcat
     4081 gacaaaggta agacgcactc ttccggcaaa ttgttattct ccgcccgcat cattccctac
     4141 cgtggttcat ggttggattt tgaatttgat ccgaaagatt tgctgtattt ccgtatcgac
     4201 cgccgccgta aaatgccggt aacgattttg ttgaaggctt tgggctacaa caatgagcaa
     4261 atcttggata ttttctacga caaagaaacg ttctatttgt cttcaaacgg tgttcaaacc
     4321 gatttggtcg caggccgtct gaaaggcgaa actgccaagg tcgatatctt ggataaagaa
     4381 ggcaatgtat tggttgccaa aggtaagcgt attactgcga aaaatatccg tgatattacc
     4441 aatgcaggcc tgacccgttt ggatgtagaa ccggaaagcc tgctgggcaa agcattggct
     4501 gccgatctga ttgattcgga aaccggcgag gtattggctt ctgccaatga tgaaattaca
     4561 gaagagttgt tggccaaatt tgatatcaac ggcgtaaaag aaattacgac cctttacatc
     4621 aatgagttgg atcagggtgc ttatatctcc aataccttgc gtacggatga gactgccggc
     4681 cggcaggcgg ctcgtgttgc gatttaccgt atgatgcgtc cgggcgaacc gcccactgaa
     4741 gaggcggtcg agcaattgtt taaccgcttg ttcttcagtg aagacagcta cgatttgtcc
     4801 cgcgtaggcc gtatgaaatt taatacgcgc acatacgaac aaaaactgtc cgaagcccaa
     4861 caaaactctt ggtacggccg cctgctgaac gaaacgtttg ccggtgctgc cgacaaaggc
     4921 ggttatgtcc tgagcgtcga agatattgtc gcctcgattg cgactttggt cgagttgcgt
     4981 aacggccatg gcgaagtgga cgatatcgat cacttgggca accgccgagt acgttcggta
     5041 ggcgagctga ctgaaaacca attccgcagc ggtttggccc gtgtggaacg tgctgtaaaa
     5101 gaacgtttga atcaggcgga atcagaaaac ttgatgccgc acgatttgat taatgcaaaa
     5161 cctgtttctg ccgctattaa agaattcttc ggctccagcc aattgagtca gtttatggat
     5221 cagaccaacc ccttgtctga agtaacccat aaacgccgcg tatctgcatt gggtccgggc
     5281 ggtttgaccc gcgaacgtgc cggcttcgag gtacgggacg tgcatccgac ccactacggc
     5341 cgcgtatgtc cgattgaaac gcctgaaggt ccgaacatcg gtttgatcaa ctcattgtcc
     5401 gtttatgcgc gcaccaatga ttacggtttc ttggaaacgc cttaccgccg cgttatcgac
     5461 ggcaaagtaa ccgaggaaat cgattacttg tctgccatcg aagaaggccg ctatgtgatt
     5521 gcacaggcga atgccgattt ggattcagat ggcaatctga ttggcgattt ggttacctgt
     5581 cgtgaaaaag gcgaaaccat tatggcaacg cccgaccgcg tccaatatat ggacgtggca
     5641 accggccagg tggtatccgt tgcggcatcc ctgattccgt tcttggaaca tgatgacgcg
     5701 aaccgcgcat tgatgggtgc caacatgcaa cgtcaggcag tgccttgctt gcgtcctgaa
     5761 aaaccgatgg tcggtaccgg tatcgagcgt tccgttgccg ttgactctgc tactgcaatc
     5821 gttgcccgcc gaggcggcgt ggtcgagtat gtcgatgcca accgtgttgt gattcgtgtc
     5881 catgacgacg aagcgactgc cggcgaagtg ggtgtcgata tttacaactt ggttaaattt
     5941 acccgttcca accagtctac caatatcaat cagcgtcctg ccgtcaaagc cggcgatgtt
     6001 ttgcaacgcg gcgatttggt ggccgatggc gcgtccaccg atttgggcga attggctttg
     6061 ggtcaaaata tgaccatcgc cttcatgccg tggaacggtt acaactacga agactcgatt
     6121 ctgatttccg aaaaagtggc tgcggatgat cgttacactt cgattcacat tgaggaattg
     6181 aatgtcgttg cccgcgatac caagctgggt gcggaagaca ttacccgcga tattccgaac
     6241 ttgtccgagc gtatgcaaaa ccgcttggac gaatccggta tcgtttacat cggtgcagaa
     6301 gtagaagccg gcgatgtgtt ggtaggcaaa gtaacgccta aaggcgaaac ccaactgacg
     6361 ccggaagaaa aactgttgcg cgccatcttc ggcgaaaaag cgtctgacgt aaaagatact
     6421 tcactgcgta tgcctaccgg tatgagcggt acggttatcg acgttcaagt cttcactcgt
     6481 gaaggtattc aacgcgacaa acgcgcccaa tccattatcg attccgagtt gaaacgctac
     6541 cgtttggatt tgaacgacca attgcgtatt ttcgacaacg acgcattcga ccgtatcgaa
     6601 cgtatgattg tgggtcaaaa agccaatggt ggtccgatga agctggctaa aggcagcgaa
     6661 attactaccg aatatttggc gggtctgccg agcaggcacg attggttcga tatccgtctg
     6721 accgatgaag atttggccaa gcagttggaa ctgattaaag tgagcctgca acaaaaacgc
     6781 gaagaagcgg acgagttata cgaaatcaag aagaaaaaac tgacccaagg cgacgaattg
     6841 caacccggcg tacaaaaaat ggtgaaagtt tttatcgcca tcaaacgccg tctgcaagcc
     6901 ggcgacaaaa tggcgggccg ccacggtaac aaaggcgtgg tatcgcgcat tctgccagtg
     6961 gaagacatgc cttacatggc ggacggccgt ccggtagaca tcgtactgaa cccattgggc
     7021 gtaccttccc gtatgaacat cggtcagatt ttggaagttc acttgggttg ggcagcaaaa
     7081 ggtatcggcg agcgtatcga ccgtatgctg aaagagcaac gcaaagcagg cgagttgcgc
     7141 gagttcttga acagactcta caacggcagc ggtaagaaag aagatttgga cagcctgaca
     7201 gatgaagaaa tcatcgaatt ggcttccaac ctgcgtaaag gtgcatcttt cgcttctcct
     7261 gtattcgacg gtgcgaaaga gtctgaaatc cgcgaaatgt tgaacttggc ttacccaagc
     7321 gaagatcctg aggttgagaa actgggcttc aacgacagta aaactcaaat cacgctgtat
     7381 gacggccgtt caggcgaagc atttgatcgc aaggttacag tcggtgtgat gcactatctg
     7441 aaactgcacc acttggttga cgaaaaaatg cacgcccgtt ctaccggtcc atacagtctg
     7501 gttacccaac agcctttggg cggtaaagct cagttcggtg gccaacgttt cggtgagatg
     7561 gaggtttggg cattggaagc atacggtgcg gcatacacgc tgcaagagat gctgactgtg
     7621 aagtctgatg acgtgaacgg ccgtaccaaa atgtacgaga acatcgtcaa aggcgaacac
     7681 aaaatcgatg ccggtatgcc cgagtccttc aacgtattgg ttaaagagat tcgttcactg
     7741 ggcttggata tcgatttgga acgctactaa gcagaagttt tcagacggcc tttcaaggtc
     7801 gtctgaaaaa gcggtttcag aataagaatg aagcaatcgg catttaggcc gtctgaaatc
     7861 gaaagtaccg tttcccaata tcgaaaatcc gccacgcggt aaaaatactt ccttcaagga
     7921 gcaaaaatga atttgttgaa cttatttaat ccgttgcaaa ctgccggcat ggaagaagag
     7981 tttgatgcca tcaaaatcgg cattgcttct cccgaaacca tccgttcatg gtcttacggc
     8041 gaagttaaaa aacctgaaac catcaactac cgtacgttca aacctgagcg tgacggtctg
     8101 ttctgcgcca aaatctttgg tccggtcaaa gactacgaat gcttgtgcgg aaaatacaaa
     8161 cgcttgaaat ttaaaggcgt aacctgtgaa aaatgcggcg tagaagtgac cctgtccaaa
     8221 gtgcgccgcg aacgcatggg ccacattgaa ttggcagcac ccgttgccca tatttggttc
     8281 ttgaaatccc tgccttcccg cttgggtatg gtactggaca tgactttgcg cgacatcgaa
     8341 cgcgtattgt actttgaagc atttgtggta accgaccccg gtatgactcc gctgcaacgc
     8401 cgccaattgc tgactgaaga cgattactac aacaaactgg acgaatacgg cgatgacttc
     8461 gatgccaaaa tgggtgcgga aggtatccgc gaattgctgc gtaccttgaa tgtggcgggc
     8521 gaaatcgaaa tcctgcgcca agagttggaa tcgaccgggt ccgacaccaa aatcaaaaaa
     8581 atcgccaaac gtttgaaagt attggaagcc ttccaccgtt ccggtatgaa gctggaatgg
     8641 atgattatgg atgtgctgcc ggtattgccg cctgatttgc gtccgttggt tccattggat
     8701 ggcggtcgtt ttgccacttc cgatttgaac gatttgtacc gtcgcgtcat caaccgtaac
     8761 aaccgtctga aacgtctgtt ggaactgcat gcgcccgaca ttatcgtccg caacgaaaaa
     8821 cgtatgttgc aagaagcggt cgactctctg ttggataacg gccgtcgcgg taaagccatg
     8881 accggcgcca acaaacgccc gctgaaatca ttggcagaca tgattaaagg taaaggcggt
     8941 cgcttccgtc aaaacctgtt gggcaaacgt gtggactact ccggccgttc cgtgattacc
     9001 gtaggcccat acctgcgtct gcaccaatgc ggtctgccga aaaaaatggc tttggaactg
     9061 ttcaaaccgt tcattttcca caaattggaa aaacaaggtt tggcctctac cgttaaagca
     9121 gcgaaaaaat tggtagagca agaagtaccg gaagtatggg acatcttgga agaagtcatc
     9181 cgcgaacatc cgattatgct gaaccgtgcg ccgaccctgc accgtttggg tattcaagcg
     9241 ttcgaaccta tcttgattga aggtaaagcg attcagttgc acccattggt gtgtgctgcg
     9301 ttcaacgccg actttgacgg cgaccaaatg gcggtacacg ttccattgag cttggaagca
     9361 caaatggaag cacgcacgct gatgctggct tcaaacaacg tattgtctcc tgccaacggc
     9421 gaaccgatta tcgtaccttc ccaagacatc gtattgggcc tgtactacat gacccgcgac
     9481 cgtatcaatg ccaaaggcga gggcagcctg tttgccgatg tgaaagaagt gcatcgcgca
     9541 taccatacca aacaggttga actgggtacg aaaattaccg tacgtctgcg cgaatgggtg
     9601 aaaaacgaag caggtgagtt tgagcctgtc gttaaccgtt acgaaacaac cgtcggccgt
     9661 gcattgttga gcgaaatcct gccgaaaggc ctgccgtttg aatatgtcaa caaagcgttg
     9721 aagaaaaaag aaatttctaa actgattaac gcatcgttcc gcctgtgcgg cttgcgcgat
     9781 acggttatct ttgctgacca cctgatgtac accggtttcg gatttgcggc aaaaggcggt
     9841 atttccattg ccgttgacga tatggaaatt ccaaaagaaa aagtggcctt gctggctgaa
     9901 gccaatgccg aggttaaaga aatcgaagac caataccgtc aaggtttggt taccaacggc
     9961 gaacgctaca acaaggtggt cgatatttgg ggtcgtgccg gcgataagat tgctaaagcg
    10021 atgatggaca acttgtccaa acaaaaagtt atcgaccgtg acggcaacga agtcgatcaa
    10081 gagtcattca actccattta tatgatggcg gactccggtg cccgtggttc tgcagctcag
    10141 attaaacagt tgtccggtat gcgtggcttg atggcaaaac ctgacggctc gattattgaa
    10201 acgccgatta cctcaaactt ccgtgaaggt ctgaccgtat tgcaatactt tattgcgacc
    10261 cacggtgcgc gtaagggttt ggcggatacc gcattgaaaa ccgcgaactc cggttacctg
    10321 acccgtcgtc tggtagacgt aactcaagat ttggtcgttg ttgaagacga ttgcggtact
    10381 tcagacggct ttgtcatgaa ggcagtggta caaggcggtg atgtgattga agcattgcgc
    10441 gagcgtattt tgggccgtgt tactgcgtct gacgttgtcg atccgtcaag cggcgagacc
    10501 ttggttgaag caggcacgtt gttgactgaa aaactggtgg atatgattga ccaatccggt
    10561 gtcgatgaag tcaaagtccg tactccgatt acttgtaaaa cccgccacgg tttgtgcgcg
    10621 cactgttacg gccgcgactt ggcgcgcggc aaactggtca atgccggaga ggcagtcggc
    10681 gtgattgccg cccaatcaat cggcgaaccg ggtacccagc tgactatgcg tacgttccac
    10741 atcggtggtg cggcatcccg tgcggcagca gccagccaag tggaagccaa atccaacggt
    10801 acggcacgat tcagcagcca aatgcgttat gttgccaaca ataaaggcga gttggttgtc
    10861 atcggccgtt cttgtgaagt cgtgattcac gatgacatcg gccgtgaacg cgaacgccac
    10921 aaagtacctt acggtgccat cctgctggta caagacggta tggccattaa agccggtcaa
    10981 accttggcaa cctgggatcc gcatacccgt ccgatgatta ccgaacacgc aggtatggtg
    11041 aaattcgaaa acgtggaaga gggcgttacc gttgccaaac aaaccgatga tgtaaccggt
    11101 ttgtccactt tggtagtgat cgacggtaaa cgtcgttcct ctagtgcttc caaactgctg
    11161 cgtccgactg tgaaactctt ggacgaaaac ggcgtggaaa tctgtattcc cggtacttct
    11221 actccggtat ccatggcatt ccccgttggt gcggtgatta ccgtacgcga aggtcaggaa
    11281 atcggtaaag gcgacgtatt ggcgcgtatc ccgcaagcct cttccaaaac ccgcgacatt
    11341 accggtggtc tgccgcgcgt tgccgaattg tttgaagcac gcgtgccgaa agatgccggt
    11401 atgttggcgg aaattaccgg taccgtttcc ttcggcaaag agaccaaagg caagcaacgt
    11461 ctgattatta ccgacgtgga cggcgtagca tacgagactt tgatttccaa agagaaacag
    11521 attttggtac acgacggtca agtggtaaac cgcggcgaaa ccattgtgga cggcgcggtc
    11581 gatccgcacg atattctgcg tctgcaaggt atcgaagcac tggcacgcta cattgtccaa
    11641 gaggtgcaag aggtttaccg tctgcaaggt gtgaagattt ccgacaaaca catcgaagtc
    11701 atcatccgtc aaatgttgcg ccgtgtgaac attgcggatg ccggcgaaac cggcttcatt
    11761 accggcgagc aggtcgaacg cggcgatgtg atggctgcca atgaaaaagc tttggaagaa
    11821 ggcaaagaac cggcacgtta cgaaaacgta ttgctgggta ttaccaaagc ctccctgtct
    11881 accgacagct tcatttctgc cgcatcgttc caagaaacga cccgcgttct gaccgaagcc
    11941 gcgattatgg gcaaacaaga cgagctgcgc ggtctgaaag agaacgtgat tgtcggtcgt
    12001 ttgattcctg ctggtactgg tttgacttac caccgcagcc gtcatcaaca atggcaagag
    12061 gtggagcagg agactgctga aacccaagta acggatgaat aatctttggt gcgtttattc
    12121 aatacaaaac cgcaagcttt aggcttgtgg tttttctttc agacggcatt taaattatta
    12181 gtgatttcct ttccggtaat agttgtttat gagcataagg gacctaaatg tttatctgaa
    12241 aaaagaaaaa tgatgaatct tcctgatctc acgcttgact gtctgcttgc aataaaaata
    12301 taattccact cttgccgaca tggtgtcggc aagtatttaa ctcaacagga cgagaaaata
    12361 tgccaactat caaccaattg gtacgcaaag gccgtcaaaa gcccgtgtac gtaaacaaag
    12421 tgcccgcact ggaagcctgc ccgcaaaaac gcggcgtgtg cacccgtgta tacacgacta
    12481 cccctaaaaa acctaactct gcattgcgta aagtatgtaa agtccgcctg accaacggtt
    12541 ttgaagtcat ttcatacatc ggcggcgaag gccacaacct gcaagagcac agtgtcgtat
    12601 tgattcgcgg cggtcgtgta aaagacttgc caggtgtgcg ttaccacact gtacgcggtt
    12661 ctttggatac tgcaggcgtt aaagaccgta aacaagcccg ttccaaatac ggtgctaagc
    12721 gtcctaaata attactggga cttaaatagg cacgtcggcc gcctaagctg aacaacggcc
    12781 gagtaagtga atactcaatt gggtattcat gggaatagac ccgactgaat agattaaagg
    12841 aaattaaaat gccaagacgt agagaagtcc ccaagcgcga cgtactgcca gatcctaaat
    12901 tcggcagcgt cgagttgacc aaattcatga acgtattgat gattgacggt aaaaaatccg
    12961 ttgccgagcg tatcgtttac ggtgcgttgg aacagattga gaaaaaaacc ggcaaagtag
    13021 caatcgaagt atttaacgaa gccattgcaa acgccaagcc tatcgtggaa gtgaaaagcc
    13081 gccgtgtagg tggtgcaaac taccaagttc ctgttgaagt tcgtccttca cgccgtttgg
    13141 ctttggcaat gcgctgggtt cgcgatgcgg cccgcaaacg tggtgagaaa tccatggacc
    13201 tgcgtttggc aggcgaattg attgatgcgt ccgaaggtcg tggcggtgcg ttgaaaaaac
    13261 gtgaagaagt acaccgtatg gctgaagcca acaaagcatt ctctcacttc cgtttctaat
    13321 tttgaaaggc taataaaatg gctcgtaaga ccccgatcag cctgtaccgt aacatcggta
    13381 tttccgccca tatcgatgcg ggtaaaacca cgacgacaga acgtattttg ttctatactg
    13441 gtttgaccca caagctgggc gaagtgcatg acggtgcggc tactaccgac tacatggaac
    13501 aagagcaaga gcgcggtatt accattacct ccgctgccgt tacttcctac tggtccggta
    13561 tggcgaaaca attccccgag caccgcttca acatcatcga caccccggga cacgttgact
    13621 ttaccgtaga ggtagagcgt tctatgcgtg tattggacgg cgcggtaatg gtttactgcg
    13681 cggtgggcgg tgttcaaccc caatctgaaa ccgtatggcg gcaagccaac aaataccaag
    13741 tgccgcgctt ggcgtttgtc aataaaatgg accgtcaggg tgccaacttc ttccgcgttg
    13801 tcgagcaaat gaaaacccgt ttgcgcgcaa accctgtacc tatcgtcatt ccggttggtg
    13861 cggaagacaa cttcagcggt gtggttgatt tgttgaaaat gaaatccatc atttggaatg
    13921 aagccgataa aggtacaacc tttacctatg gcgatattcc tgccgaattg gtcgaaactg
    13981 ccgaagaatg gcgtcaaaat atgattgaag ccgcagccga agccagcgaa gaactgatgg
    14041 acaaatactt aggcggcgac gagctgaccg aagaagaaat cgtaggcgcg ttgcgtcaac
    14101 gtactttggc aggcgaaatt cagcctatgc tgtgtggttc tgcatttaaa aacaaaggtg
    14161 ttcaacgtat gttggacgca gttgtagaat tgctgccagc tcctaccgat attcctccgg
    14221 ttcaaggtgt caacccgaat accgaggaag ccgacagccg tcaagccagc gatgaagaga
    14281 aattctctgc attggcgttc aaaatgttga acgacaaata cgtcggtcag ctgaccttta
    14341 tccgcgttta ctcaggcgta gtaaaatccg gcgataccgt attgaactcc gtaaaaggca
    14401 ctcgcgaacg tatcggtcgt ttggtacaaa tgactgccgc agaccgtact gaaatcgaag
    14461 aagtacgcgc cggcgacatc gcagccgcta ttggtctgaa agacgttact accggtgaaa
    14521 ccttgtgtgc ggaaagcgcg ccgattatct tggaacgtat ggaattcccc gagccggtaa
    14581 tccatattgc cgttgagccg aaaaccaaag ccgaccaaga gaaaatgggt atcgccctga
    14641 accgcttggc taaagaagac ccttctttcc gtgtccgtac agacgaagaa tccggtcaaa
    14701 ccattatttc cggtatgggt gagctgcact tggaaattat tgttgaccgt atgaaacgcg
    14761 aattcggtgt ggaagcaaat atcggtgcac ctcaagtggc ttaccgtgaa actatccgca
    14821 aagccgttaa agctgaatac aaacacgcga aacaatccgg tggtaaaggt caatatggtc
    14881 acgttgtgat tgaaatggaa cctatggaac cggggggcga aggttacgag tttatcgatg
    14941 aaattaaagg tggcgtgatt cctcgcgaat tcattccgtc tgtcgataaa ggtatccgcg
    15001 atacattgcc taacggtatc gttgccggct atcctgtagt tgatgtacgt atccgtctgg
    15061 tattcggttc ttaccatgat gtcgactctt cccaattggc atttgaattg gccgcttctc
    15121 aagcgtttaa agaaggtatg cgtcaagcat ctcctgccct gcttgagccg attatggcag
    15181 ttgaagtgga aactccggaa gaatacatgg gcgacgtaat gggcgacttg aaccgccgtc
    15241 gcggtgttgt attgggtatg gatgatgacg gtatcggcgg taaaaaagtc cgtgccgaag
    15301 tacctctggc agaaatgttc ggttattcga ccgacctgcg ttctgcaacc caaggccgcg
    15361 ctacttactc tatggagttc aagaaatatt ctgaagctcc tgcccacata gctgctgctg
    15421 taactgaagc ccgtaaaggc taatcaggca aataggtcgt ctgaaaggct gaaatgattt
    15481 ttcagacggc attgttcttt aatcgatctt taatgtaaag gaattagc
//
LOCUS       DAWEWP010000036        14929 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWEWP010000036 DAWEWP010000000
VERSION     DAWEWP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.036"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="WGN53_000821"
     CDS             complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="WGN53_000821"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0139835.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAASPKAEAPVAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKNGVVSRLTGGLAGMAK
                     GRKVDVIQGDGQFLDSHHLEVSLTAGDAYELAAPTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1968..3569)
                     /gene="aceF"
                     /locus_tag="WGN53_000822"
     CDS             complement(1968..3569)
                     /gene="aceF"
                     /locus_tag="WGN53_000822"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ0139836.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAPAAAPVPAAAAAPAPSAP
                     AAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIMGDDIKAFVKSVMQGG
                     AAKPAAASASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVT
                     VHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLV
                     LKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGAC
                     FTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRV
                     IDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3719..6382)
                     /gene="aceE"
                     /locus_tag="WGN53_000823"
     CDS             complement(3719..6382)
                     /gene="aceE"
                     /locus_tag="WGN53_000823"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ0139837.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7076..8788
                     /locus_tag="WGN53_000824"
     CDS             7076..8788
                     /locus_tag="WGN53_000824"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ0139838.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERTAAQAELTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVESENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASSAAIVEQFGANGG
                     SLGPVGFKGKVYADFATEKGADWVIGANEDGYHYTSFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     LLNG"
     gene            8851..9507
                     /locus_tag="WGN53_000825"
     CDS             8851..9507
                     /locus_tag="WGN53_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ0139839.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9517..9972)
                     /gene="ruvX"
                     /locus_tag="WGN53_000826"
     CDS             complement(9517..9972)
                     /gene="ruvX"
                     /locus_tag="WGN53_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ0139840.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9965..10513)
                     /locus_tag="WGN53_000827"
     CDS             complement(9965..10513)
                     /locus_tag="WGN53_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ0139841.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10515..11075)
                     /locus_tag="WGN53_000828"
     CDS             complement(10515..11075)
                     /locus_tag="WGN53_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ0139842.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11403..13223
                     /locus_tag="WGN53_000829"
     CDS             11403..13223
                     /locus_tag="WGN53_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0139843.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSVRKGHEGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLK
                     TEVAATKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKD
                     LEKQQKALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDA
                     RKLLEQKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKA
                     EARRAEMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGD
                     VWKGVFYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKG
                     YMVAAGSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13350..14834
                     /locus_tag="WGN53_000830"
     CDS             13350..14834
                     /locus_tag="WGN53_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ0139844.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAEQAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLNGAVGVSAAFLPPEAVVVSTKGRDG
                     KDRMVLKAVPEDYVYGMGGDPLAGIPAELKTVPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3555 a         3704 c         4198 g         3472 t
ORIGIN      
        1 ccggaagaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgcagc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggacaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcggcg gcaacgtggc
      541 cttcgtgaac ggctttgtgc gccaacatcg gctgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggttacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttag gcgcatttgc accttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttta ccaaatcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgagaccgat aataccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggtaacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagggg
     1141 ctgccagttc gtacgcgtcg ccggcagtca gcgatacctc caagtggtgc gaatctaaga
     1201 attggccgtc gccttggata acatccactt tacggccttt cgccatacct gccaaaccgc
     1261 cggtgaggcg ggaaaccacg ccgtttttgt aggcgcgcag catatcgatg tcgagttcag
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacag ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaggttt
     1441 tataacgttc gacgatggcg actttcaagc cttcatcggc agcagcaaat gcagcggagt
     1501 aaccgccggg accgccgccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagcg ggagcggcag ctttgggtgc ttcttgcgca ggggcagcaa
     1621 caggtgcttc ggctttagga gaggcagccg cgccttcggc ttcaacgact acaatcaagc
     1681 cgccttcgga gattttgtcg ccgactttga ctttcacttc tttgaccacg cccgcaactt
     1741 cggcaggcac gtccatcgtc gctttgtcgg tttccaaggt aatcagggtg tcgtcaacgg
     1801 cgatggtatc gcccacattc acttcaaccg cgataatatc tacattttcg tgtccgccaa
     1861 tgtcgggcac tttcaattca actaagctca tgtctcatct ttctgaatga tgttcaaact
     1921 tccttttcag acggcacagg ccgctatgcc gtctgaagcc gatcgggtta cagggtaatg
     1981 cggcggaagt ctttcaacag cttcgccaag aatacggtga agcgcatacc ggccgcaccg
     2041 tcgatgacac ggtggtcgaa ggacaggctc aacgggcaca tcaggcgcgg ggcaaactct
     2101 ttgccgttcc aaacaggttt gatttgggat ttgcacacgc ccaagatggc gacttcggga
     2161 gcgttcacaa ttggcgtgaa gcctgtgccg ccgatgccgc ctaagctgga aatggtaaag
     2221 cacgcgcctt gcatttcttg cggtttgagc ttgccttcac gggctttttt ggacaattcg
     2281 gtcaattctt ggctgatttg tttcaagcct ttttgatcca cgtctttgat gacgggaaca
     2341 accaagccgt tcggcgtatc ggctgcgaaa ccgatgttga agtagttttt cagcaccagg
     2401 ttgtcgccgt ccagtgaggc gttgaattcg gggaatgctt tcaacgcgga aacagaggct
     2461 ttgatgatga acgccaacgg ggacagtttc acgccttcgc gttcccattc tttgttcagc
     2521 tgtttgcgga attcttccag ctcggtcatg tccgcttctt cgtgtacggt aacgtgggga
     2581 atcacaaccc agttgcggga caggttttga ccggaaattt tcttaatgcg ggacaattct
     2641 ttaacttcga cattgccgaa tttggagaag tccactttag gccacggcag taagtccaga
     2701 ccgccgccca aagatgcgct ggctgcggca ggttttgccg cgccgccctg catcacggat
     2761 ttcacaaagg ctttgatgtc gtcgcccatg atacggcctt tcaagccggt gcctttgact
     2821 tggcccaaat ccacgcccaa ttcgcgcgcc agtttgcgtg cggaaggacc ggcgtgtgct
     2881 ttggcgaaag cggcctcgtc gattttggca gcggcaggtg cagaaggtgc gggcgcggcg
     2941 gcagcagccg gaaccggagc ggcagccggt gcgggtgcgg cagcttgagc ggcaggcgca
     3001 ggggctgctg cggcagagcc ggcggtttct acttcgataa ttgcagagcc ttcggacact
     3061 ttgtcgccga cttttaagaa tacggcttta acgacaccgg cagcggtaca aggtacgtcc
     3121 attgtcgctt tgtcggtttc caaagtaatc agcgtgtcgt cttcggcaac ggtgtcgccc
     3181 actttgattt caacggcgat gacatccaca tcggtatggc cgccgatatc gggaacggct
     3241 acttgaacgg ttgcaccgcc tgcggcaacg ggtgcggcag caggtgcagg ttgtgcttcg
     3301 gcagcagcag gagcggcttc ggcagcggcg gcaccggttt caacggtcaa aattacgccg
     3361 ccttcggaga ttttgtcgcc gactttgact tttacttctt tcacgacacc ggccgcatcg
     3421 gcaggcacat ccatcgtggc tttgtcggtt tccagtgtaa tcagggtgtc gtcaacggcg
     3481 atggtgtcgc ccgctttaac ttctacggcg atgatgtcga cgttttcgtg accgccgata
     3541 tcggggactt tgatttctac gatactcatt tgagttcctt taatcttgtt cagttggatg
     3601 ccgtctgaaa gctgcttcag gcggcatcgg tacggtttgt ctgctcaaac cattcgggca
     3661 ttcggttttc agacggcctg atgtcggaat ggggcaaaca gaccgtctga aacatcaatc
     3721 agcgtttcca gctaggagct gaatcggctt tgatgccgta tttctcaatg gcttgttgaa
     3781 cggtttcttt gctgactttg ccttgttccg ccaatgcgct caatgctgcc acggcaacgt
     3841 tgtagcgatc cacttcaaag aagcggcgca ggttggcgcg gctgtcggag cggccgaagc
     3901 cgtcagtacc caagacgtgg tagtcgttcg ggatgtaggc gcggatacgg tcagcatagc
     3961 tgcggatata gtcggtagcg gcaataaccg gaccgtcatg accttgcaat ttggaagtaa
     4021 cgaaaggtac tttttcggct tccagcggat gcaggcggtt gaagcgttct acctcgacag
     4081 cgtcgcggtg cagcaggttg aaggacgggc aagaccaaat gtctgcttcc acgccgaagt
     4141 cggctttcag caattcggca ccggcaatga cttcttgcag gatggtaccg gagcccatca
     4201 attgaacttt cttatcgcct ttgccgccgg ctttcagcag gtacatacct ttcaagatgt
     4261 cttgttctgc gccttcgggc atatccggat gggtgtagtt ctcgttcatc agggtgatgt
     4321 agtagaacac gtcttcatta ttggcataca tacggcgcag accgtcttgt acgatgacgg
     4381 cgacttcgta ttggaaagtc gggtcataag atacgcagtt cggaatcagg tcggcctgga
     4441 tgtggctgtg gccgtcttcg tgttgcaggc cctcgccgtt cagcgtcgta cggccggcag
     4501 taccgcccag caggaagccg cgcgcgtgca tatcgcccgc cgcccaagcc aagtcgccga
     4561 tacgttggaa accgaacata gaatagtaaa tgtagaacgg aatcatcgcg aagttgctgt
     4621 tggcgtagct ggttgcagcc gcaatccagt cggccatcgc gcccggttcg ttaatacctt
     4681 cttgcaagat ttgaccgtca acggattctt tatagaacat cagttggtct ttgtcttgag
     4741 gggtatattg ctgacctttc ggattccaaa taccgtattg gcggaacata ccttccatgc
     4801 cgaaagtacg gctttcgtcg ggaacgatag gtacgacgcg tttgccgatt tttttgtctt
     4861 tcagcaaagt ggacaggatg cggacgaatg ccatcgtggt cgagaactcg cgttcaccgc
     4921 tggatttgag ttgtgcgtcg aatgctgaca gctcgggcac ttccaatact tcctgcgtcg
     4981 gtttgcgttg cggcaggtag ccgcccaaag catcgcggcg tgcgtgcagg tatttgtatt
     5041 cttccgtatc gggggcaaaa gtcaggtaag gcagatcgcc gctttcgatt tgttcgtcgg
     5101 taaccggaat gtcaaagcgg tcgcggaatt gtttcaggga cgctttgtcc atttttttgg
     5161 cttggtgggc aacgttctga ccttcgccgg atgcgcccat accgtaacct ttaatggttt
     5221 tcgccaagat gacggtaggt ttgccgtcgg catggttcgc tgcgcggtcg taggcgttgt
     5281 acactttttg cgggtcgtgg ccgccgcggt tcaatgccca gagttgctca tcggtcatat
     5341 cggcaaccaa tgctttcagt tcgggcgtat tgaagaagtg ttcgcgcaca tacgcgccgt
     5401 ctttggattt gtaagtttgg tagtcaccgt ccaaacattc ttccatacgt tggcgcagga
     5461 taccgtcttt gtctttcgcc aagaggcggt cccagcggcg gccccaaatg actttgacga
     5521 cattccagcc ggcgccggca aagttgcctt ccaattcttg gatgattttg ccgttgccgc
     5581 gtaccggacc gtccaagcgt tgcagattgc agttgatgac gaaaatcagg ttgtccaagc
     5641 cttcgcgtgc agccagtgcg attgcacctt gagattcggg ttcgtccatt tcgccgtcgc
     5701 cgcagaaaca ccatacttta cggcctttgg tttttgccaa accacgcgat tccaagtatt
     5761 tcaggaaacg cgcctgataa atcgccatga tgggccccaa gcccatggat acggtcggga
     5821 actgccaaaa gtcgggcaag aggtgggggt gcggatagga aggcagaccg tgtccgtcca
     5881 cttcttggcg gaagttgttc agctggtctt cggtcagacg gccctcgacg aatgcgcgtg
     5941 catagatgcc cggggcgacg tgaccttgga agaagaccaa atcgccttct tcgccttcgc
     6001 ctttggcttt ccaaaagtgg ttgaaaccga cttcgtacat ggtggcggca gattggaaag
     6061 atgcgatgtg cccacccagt tccaaatctt tcttgccggc gcgcaatacg atggcggcgg
     6121 cgttccagcg cacgaatgcg cggatgcggt gttcgatgtt ttggtcgccc ggaatgcctt
     6181 tttcgttttc aaccgaaacg gtattcaaat acggtgtggt cgtgccgtgt ggcatacgta
     6241 cgcccttgtc gcggcagtat ttgaccaggt tttccaagag gtattgcgcg cgttcgccgc
     6301 cttcatattc gaggacggag cttaacgcgt ccagccactc ttgggtttcg atagggtcaa
     6361 catcgtgtaa ttgggtggac atagtatcta tcctttatgt tgagtgttta atcggcaggg
     6421 agggtgtagc caaccccttt attttcgttt gtgaaaaata ataagcaaaa acgaaaattt
     6481 ctaaatcaaa ccgaaaaaaa taatttaagt ttaatgtttt caaaaaatta taaaagtttt
     6541 ggaaacaagg gaaaacttga aaaaaatgtt cgatctgctc cacacgaaat cctatcagca
     6601 atcgggtctg ctggcaaata accatgcctg taaaagcagg ttttatactg aagttggcgt
     6661 ttggtaaggt caagtctttt tgatttcgaa atattctgtt tgattttggc gatatttttt
     6721 cggaattgtg gtatatcgaa tcattatgtc tctgtcggca attttcctat tgtagaaaat
     6781 ctatacacaa gctttgtgga taaccgtgtt gataagccac ggcttgtgtt aaaaaagtct
     6841 tacgcaacaa atgtacgctt attttgatta aaaaataggc acatttaaat ctataaaaat
     6901 catgtgctta tgttcaatat gatgtttctg gtcatgtttt tgccgtctgt tatgggattt
     6961 gtcccgcgct tggggcaggg ggtaaatttt ggaggttggt gcaaaacctg cttttttcgc
     7021 gttacaatct gcacccgatt tacttttgaa tttgcctgtt tggaaaaata tttttatgaa
     7081 agccagccaa ttctttatct ctactttaaa agaagcccct gccgaagccg cgcttgccag
     7141 ccacaagctg atgatacgcg ccggtctgat taaagccaac gcgtccggcc tttatacttg
     7201 gatgccgatg gggctgcgcg tgttacgcaa agtcgaaaac gtcgtgcgcg aggaaatggc
     7261 gcgcgcgggc agcgtggagc tgctgatgcc ggtggtgcag cctgccgagc tgtggcagga
     7321 atccggccgc tgggaatttt acggtaaaga actgctgcgc ctgaaagacc gccacgaccg
     7381 cgatttctgc atgggaccga cctgcgagga agtcatcgcc gacatcgtgc gcaaagaaat
     7441 caacagctac aaacagctgc cgaaaaactt ttaccacatc caaaccaaat tccgcgatga
     7501 agtgcgcccg cgtttcggcg tgatgcgcgc gcgcgagttc gtcatgaaag acgcttattc
     7561 cttccatgcc gactacgcct cgcttcagac gacctatgat gccatgtatg acgcttactg
     7621 ccgcatcttt acccgtctgg gcttggcatt ccgccccgtc gccgcagaca ccggcagcat
     7681 cggcggtacg ggttcgcacg agtttcaagt gttggcggaa agcggcgaag atgttattgc
     7741 atacagcgac acttccgatt acgccgccaa tatcgagttg gcaccgacct tgccgcttaa
     7801 aggcgaacgc accgctgctc aggccgagtt gaccaaagta catacaccaa atgtcaaaac
     7861 cattgagtct ttggttgaat tcctgaatat tccggttgaa caaaccctca aatccatcgt
     7921 ggtcgaaagc gaaaacgaag gcgaaatcgt cttattactg ttgcgcggcg accatgagtt
     7981 caacgacatc aaagccgaaa aactcgcagg cgtgaaatcg ccattgacga tggcaagttc
     8041 tgccgcgatt gtcgaacagt tcggcgcaaa cggcggctct cttggtcctg tcggcttcaa
     8101 aggcaaagtc tatgccgatt tcgctaccga aaaaggcgcg gactgggtca tcggtgcgaa
     8161 cgaagacggc taccactaca ccagcttcaa cttcgggcgc gatgccgccg agcctgaatt
     8221 cgtcgatttg cgtaatgtgg tcgaaggcga cgaaagcccc gacgggcaag gccgtctgaa
     8281 actcgcgcgc ggcattgaag tcggacacgt cttccagttg cgcgacaaat acacccaagc
     8341 catgaacgta agcttccttg acaacaacgg caaatcgcaa attatggaaa tgggctgcta
     8401 cggcatcggc atcacccgcg tcgttgccgc cgccatcgag cagaataacg acgaaaaagg
     8461 catcatctgg accaaagcaa tggcgccgtt tgaagtcgtt atcgtgccga tgaactacaa
     8521 aaaatcagac accgtacgcg aagccgccga caaaatctat gccgaattgc tggcggcagg
     8581 cgcggatgtg ctgctggatg accgcgacga acgcgcaggc gtattgctga acgattccga
     8641 gctgttgggt attccgcacc gcatcgtcat cggcgaccgc gctttgaaag aaggcaatgt
     8701 cgaatacgcc gaacgccgca acaacgaagc gcaggcagtt gcaatcggag aaattgttgc
     8761 gcgtgtaaca gctttattaa atgggtaagc ttggtcaaat gccgtctgaa aataatttca
     8821 gacggcattt ttatttgggg agaaagtgaa gtggcttcag tatttttaga aggagaagca
     8881 gtcgaggtcg gcaatatttt ctgtatcggc aggaactatg ccgcacatat tgaagagtta
     8941 aaaaacgaaa taccttccga acctgtggtg tttatgaagc cgtcaggcag cattttgaac
     9001 agcggcggta caatcttgct gcccgagttt agtagggatg tgcagtttga gtgcgaactc
     9061 gtgctgctgg tcggtaagga ttcagacggc acgggcgagg gtaaagatat tttgggatgt
     9121 gtcgcaggat atggtgtagg gctggacctt acggcacggg atatccaatg ccgtctgaag
     9181 gaaaaagggc tgccctggct gaaggcaaaa gggttcaggc attccgcgtg cgtgtcggat
     9241 tttgcggcgg caggcagaat cggcaatccc gagaatgtgt tgttttcatt aaaacaaaac
     9301 ggcgtattga aacagcgcgg ggataccggt ttgatgattt acccgattcg ggaaatttta
     9361 cacaaactgg cggcagatta cggtttgggg aaaggggatt tggtgtttac cggaacgccc
     9421 tccggcgtgg gcgcaatcgg tgcgggggac aatttggcat tggagctgga cggcttggtg
     9481 cgcgcctctt tcacggtagg gtgttaaccg cgccgcttaa ccctcacgcc cgttgaaaca
     9541 ttccgccgga ccgccctcga aaaaaccgtg caagatggct tgcgccgcca cttggtcgag
     9601 caccgatttg cgttttttgc cgaagacctg tgcttccgaa agcaggcttt cggcatagac
     9661 ggacgacagc cgttcgtcaa cccaatagac ggggagattg aacctgccgt tcagcctgcg
     9721 tccgaacttg cgcgacaggt gcgtcatttc atgtttcgtg ccgtcggtat gcacgggcag
     9781 tccgacgaca aaataacgcg gctgccattc ttgaaccagc ttggcgattg ccgcgaactt
     9841 ttcatcgttg ctgccgccgg taacggtgct caaaggatgg gatagcccta attccgcgtc
     9901 tccttgtgcc acgccgatac gcgcttcgcc gaagtcgaat gccagtgccg ttccttttgg
     9961 aattttatgc atggccggct cccgaaaaca gggcgagcgg gtcgatgccg agtttggcga
    10021 atgcggcggc gtaacggtgt tcgtagggga tgtcgaacag gatgtgttcg tcggcgggaa
    10081 cagtcagcca cgcattgtcg gcaagttcgc gttcgagctg gccttttccc cagcttgaat
    10141 agcctatgct gatcaaggct ttgtcaaccg caccttcgcg tgaaatattt tcaatcacgt
    10201 ctcgggaaga agttagcgcg atattgtctg aaacgccgat actgctttgc cagttgccga
    10261 tcggggtatg cacgacataa cctcgctcga cttgcaccgg cccgcccatc atcacgctgt
    10321 cgtgctgcat ccgcatgggg atgtttttgc cggtggcgga aaaaatcatg tccatcgtaa
    10381 tcggagaggg tttgttgatg gcgatgccga gtgcgccgtc ttcatcgtgt ttgcagatat
    10441 agacgaccga ttgtgaaaaa aacgcgtctt ccatatcggg catggcgacg agaaagtggt
    10501 tggagaggtt catatcagcg tttgtcggga ttttctatcg ggttggaaat catgcaggcg
    10561 ccggcaagca ggttgccgga agtatcggct tgcaccccat cggtttgcgg tatgccgccg
    10621 ccgaaacagg aaaccgcgct taaggaattt ttcggcgagc cttggatgat tttatcgctg
    10681 tacaccaaat aggcgaaggt tttgcgtttg gggtcgtaat aacggacaat ctgccggctc
    10741 ttgaacgcga agctcgcacc gtgtttgaaa acttctttcg gtttgcgcac ggcggtttcg
    10801 tcaaaagaaa tcgaagatgc cgtctgaacg cacgaaaccg atgcgtcgga cgcgtcctct
    10861 tccaaattga ccatttcctt caagccgcct ttttttgcat acgaaatata acaggcaacc
    10921 ccttgaacgt cgggatcgtc gaatccttcc acttcgatac ggtcgttttt gcccagtatg
    10981 ttgaaaacgg tacttgcccg tccgatttta tcggtttcgc cgctgccgca ggcagtcagc
    11041 aggacggcgg cagacagcag tagcagtctg ttcatcaaaa tttccttatc ggttgaatcc
    11101 ccgttttccg tgaaggcggc agggcttgct ttgtttggag gggcgcacgt tcccaaaatc
    11161 gggcaacata cggtatgcgg cttaatgtgc taccctgtgt gtcggaacac aatatccgtt
    11221 gcggcattga aaatatgatg cacaatataa ggctgtttga gccgattgca agtatccgcg
    11281 caaagaaggc gcaagattgg gcaaacggca ttttcagacg gcataactga cagtataatc
    11341 caaacgtccg gctgctttcg tgcagccttc agtattatcc aactttttcc gaaggtcgaa
    11401 ccatgcgcta caaacccctt ctgcttgccc tgatgctcgt tttttccacg cccgccgttg
    11461 ccgcccacga cgcggcacac aaccgttccg ccgaagtgaa aaaacagacg aagaacaaaa
    11521 aagaacagcc cgaagcggcg gaaggcaaaa aagaaaaagg caaaaatggc gcagtgaaag
    11581 ataaaaaaac aggcggcaaa gaggcggcaa aagagggcaa agagtccaaa aaaaccgcca
    11641 aaaaccgcaa agaagcagag aaggaggcga catccaggca gtctgtgcgc aaaggacacg
    11701 aaggggataa gaaatcgaag gcggaacaca aaaaggcaca tggcaagccc gtgtccggat
    11761 ccaaagaaaa aaacgcaaaa acacagcctg aaaacaagca aggcaaaaaa gaggcaaaag
    11821 gacagggcaa tccgcgcaag ggcggcaagg cggaaaaaga cactgtttct gcaaataaaa
    11881 aagtccgttc cgacaagaac ggcaaagcag tgaaacagga caaaaaatac agggaagaga
    11941 aaaatgccaa aaccgattcc gacgaattga aagccgccgt tgccgctgcc accaatgatg
    12001 tcgaaaacaa aaaagccctg ctcaaacaaa gcgaaggaat gctgcttcat gtcagcaatt
    12061 ccctcaaaca gcttcaggaa gagcgtatcc gccaagagcg tatccgccaa gagcgtatcc
    12121 gccaagagcg tatccgtcag gcgcgcggca accttgcttc cgtcaaccgc aaacagcgcg
    12181 aggcttggga caagttccaa aaactcaata ccgagctgaa ccgtttgaaa acggaagtcg
    12241 ccgctacgaa agcgcagatt tcccgtttcg tatcggggaa ctataaaaac agccagccga
    12301 atgcggttgc cctgttcctg aaaaacgccg aaccgggtca gaaaaaccgc tttttgcgtt
    12361 atacgcgtta tgtaaacgcc tccaatcggg aagttgtcaa ggatttggaa aaacagcaga
    12421 aggctttggc ggtacaagag cagaaaatca acaatgagct tgcccgtttg aagaaaattc
    12481 aggcaaacgt gcaatctctg ctgaaaaaac agggtgtaac cgatgcggcg gaacagacgg
    12541 aaagccgcag acagaatgcc aaaatcgcca aagatgcccg aaaactgctg gaacagaaag
    12601 ggaacgagca gcagctgaac aagctcttga gcaatttgga gaagaaaaag gccgaacacc
    12661 gcattcagga tgcggaagca aaaagaaaat tggctgaagc cagactggcg gcagccgaaa
    12721 aagccagaaa agaagcggcg cagcagaagg ctgaagcacg acgtgcggaa atgtccaacc
    12781 tgaccgccga agacaggaac atccaagcgc cttcggttat gggtatcggc agtgccgacg
    12841 gtttcagccg catgcaagga cgtttgaaaa aaccggttga cggtgtgccg accggacttt
    12901 tcgggcagaa ccggagcggc ggcgatgttt ggaaaggcgt gttctattcc actgcaccgg
    12961 caacggttga aagcattgcg ccgggaacgg taagctatgc ggacgagttg gacggctacg
    13021 gcaaagtggt cgtggtcgat cacggcgaga actacatcag catctatgcc ggtttgagcg
    13081 aaatttccgt cggcaagggt tatatggtcg cggcaggaag caaaatcggc tcgagcgggt
    13141 cgctgccaga cggggaagag gggctttacc tgcaaatacg ttatcaaggt caggtattga
    13201 acccttcgag ctggatacgt tgatcggatt gccgaaacgg taggctttat gcaaatcgtg
    13261 ccgatatggt gcgctgttcc gtgaagtttg ccgtttgccg tacctgtatt ttccggtttt
    13321 tggaaatcaa gagagaaagt taaagaagaa tgtcgaaacc tgtttttaag aaaatcgcac
    13381 tttatacttt gggtgcaatc agcggcgtgg ccgtcagtct ggcggtgcag ggttttgccg
    13441 ccgagaagga caggcgggat aacgaagtcc tgccggtgca atccatccgc acaatggcgg
    13501 aggtttacgg tcaaatcaag gcaaactact atcaggacaa acccgatgcc gatttgtttg
    13561 aaggtgcgat gaagggtatg gtggccggtt tggatccgca ttccgaatat atggataaaa
    13621 aaggttatgc cgagataaag gagtccacca gcggcgaatt tggcggcttg gggatggaaa
    13681 tcgggcagga agacggattt gtcaaagtgg tttcgccgat tgaggacacg cctgcggaac
    13741 aggcgggggt gaaaagcggc gatttcattg tgaaaatcga taatgtttcg acacgcggca
    13801 tgacggtcag cgaagcggtg aagaaaatgc ggggcaagcc gggtacgaag attactttga
    13861 cgctgtcgcg caaaaatgcc gacaagccga tagtcgtcaa cctgacccgt gccattatta
    13921 aagtgaaaag cgtccgccat cacctgatcg aacccgatta cggctatatc cgcgtgtcgc
    13981 agttccaaga gcggacggtc gaaagcgtca ataccgccgc aaaagagctg gtaaaggaaa
    14041 ataaaggaaa accgctcaag gggctggtgt tggatttgcg cgacgacccc ggcgggcttt
    14101 tgaacggcgc ggtcggcgtg tcggcggcgt ttttgccgcc tgaagcagtc gtcgtcagca
    14161 ccaagggacg cgacggcaaa gaccgcatgg tactgaaagc cgttcctgag gattatgtgt
    14221 acgggatggg cggcgatccg ttggcgggca ttcctgccga gttgaaaacc gttccgatga
    14281 cggtgttgat taattccggt tcggcttccg cgtcggagat tgtcgcaggt gcattgcagg
    14341 atcacaaacg cgcggtcatc gtcggtacgc agagcttcgg caaaggttcg gttcagactt
    14401 tgattccttt gtccaacggc agcgcggtca agctgacaac ggcactgtat tatacgccga
    14461 acgaccgttc tattcaggcg caggggattg ttcccgatgt cgaagtaaaa gataaggaac
    14521 gcatttttga aagccgcgag gcggatttgg tcggacacat cggcaatcct ttgggcggcg
    14581 aggatgtgaa cagtgaaacc cttgccgtgc cgcttgaaaa agatgcggat aagcccgctg
    14641 taaaagaaaa aggtaaaaag aaaaaggacg aggatttgtc ttcaaggcgg attcccaacc
    14701 ctgccaaaga cgaccagttg cggaaagctt tggatttagt caagtcgccc gagcagtggc
    14761 agaagtcttt ggggctggcg gcgaaaaagc cggtttcaaa taaagataag aaagataaaa
    14821 aagataagaa gtaggttttg acgatgccgt ctgaaaggat ttcagacggc atattatgtt
    14881 tgaaaggaca ggttatgagc gattttttac cggaatttga accgtgcgg
//
LOCUS       DAWEWP010000037        14902 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWEWP010000037 DAWEWP010000000
VERSION     DAWEWP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.037"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(87..3572)
                     /gene="smc"
                     /locus_tag="WGN53_000831"
     CDS             complement(87..3572)
                     /gene="smc"
                     /locus_tag="WGN53_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ0139845.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATEQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHYWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIVTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTLDWQERQQELVLAYETEFQNDEQHIKLDELT
                     ESVHTLDEEYIAVQEKLTQIQEQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3574..3825
                     /locus_tag="WGN53_000832"
     CDS             3574..3825
                     /locus_tag="WGN53_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139846.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            3822..4544
                     /gene="lpxH"
                     /locus_tag="WGN53_000833"
     CDS             3822..4544
                     /gene="lpxH"
                     /locus_tag="WGN53_000833"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ0139847.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            4813..6399
                     /locus_tag="WGN53_000834"
     CDS             4813..6399
                     /locus_tag="WGN53_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0139848.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6567..6770)
                     /locus_tag="WGN53_000835"
     CDS             complement(6567..6770)
                     /locus_tag="WGN53_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ0139849.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6886..7383
                     /locus_tag="WGN53_000836"
     CDS             6886..7383
                     /locus_tag="WGN53_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ0139850.1"
                     /translation="MTALTLPEDIRQQEPSALLYPLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7465..8658
                     /locus_tag="WGN53_000837"
     CDS             7465..8658
                     /locus_tag="WGN53_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0139851.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEATAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9061..9996)
                     /gene="hemC"
                     /locus_tag="WGN53_000838"
     CDS             complement(9061..9996)
                     /gene="hemC"
                     /locus_tag="WGN53_000838"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ0139852.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10115..10630
                     /gene="ybeY"
                     /locus_tag="WGN53_000839"
     CDS             10115..10630
                     /gene="ybeY"
                     /locus_tag="WGN53_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ0139853.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            10632..11456
                     /locus_tag="WGN53_000840"
     CDS             10632..11456
                     /locus_tag="WGN53_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ0139854.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11585..12964)
                     /gene="nhaC"
                     /locus_tag="WGN53_000841"
     CDS             complement(11585..12964)
                     /gene="nhaC"
                     /locus_tag="WGN53_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ0139855.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13255..14478)
                     /locus_tag="WGN53_000842"
     CDS             complement(13255..14478)
                     /locus_tag="WGN53_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ0139856.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAIAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3515 a         4198 c         3831 g         3358 t
ORIGIN      
        1 tttaaattta attcactata ccgtctgaaa acctgtatcg caacacgtca ttaaaaacgg
       61 caagattcag ccgttctgca acccgttcaa acggcttccg ccatttccaa cgcctgcttg
      121 atgtccacgg cgacgacgcg cgatacgcct ttttcctgca tggttacgcc gaccagctgc
      181 tctgccattt ccatggtcag gcggttgtgg gagatgtaga ggaactgggt ttgcgccgac
      241 atttctttga ccaggttgca gaaacgcgag gtgttggcgt cgtccagcgg ggcatcgacc
      301 tcgtccaaaa ggcagaacgg agcggggttg aggctgaaca gagcgaacac gaggctcatg
      361 gcggtgaggg ctttttcgcc gccggagagg aggtggatgg tgctgttttt cttgccgggc
      421 ggacgcgcca taatggacac accggcggtc agtaggtcgt cgcctatcat tttgagagtg
      481 gcttcgccgc cgccgaacag ggtcgggaag aaggtttgga ctttgccgtt gacggcgtcg
      541 aaggtttctt tgaaacgcgc tttggttttg tcgtcgattt gggcgatggc ttcttccaaa
      601 atggtgatgg cggcctgcac gtcttcgctt tggctgcggt agtagccgtc gcgttcgcgc
      661 gcttcttcga gttcttgcag ggcggcgagg ttgaccgcgc cgagggcttc gatttgttgc
      721 gagaggctgc cgatgctgct gttcaatact ttcggcgatt ctttcgccaa cgcttcgagc
      781 gcatccaaat cggcggcgcg ttcggtcagg ttttgatggt agcgtttggc gttgatcagg
      841 gcttcctgtt gttgcaacag ggcggtttgg gtggcggcct gaagctgtgg caactgggtt
      901 tgcagggttt gtacgcgggc gtattgttcc ctaccctgct cctgaatctg cgtgagtttt
      961 tcctgcacgg cgatgtattc ttcgtccagc gtgtgtacgg attcggtcag ctcgtcgagc
     1021 ttgatgtgct gctcgtcgtt ttggaactcg gtttcatagg cgaggacaag ctcttgctgg
     1081 cgttcctgcc agtcgagggt ttgctgttca agctgggcga tttgctgccg gtagttttgt
     1141 ttttgctggt tgagtttgtg gacggcgact tcggcaagcc catattggcg gttggcttcc
     1201 aacagggcaa gctgcgcctg tttcagacgg ccttgctgct cttggcggct gtgcgcggtg
     1261 gtttgctgct ggtgttcgag ttcggcggcg gcttcctgca aggtaacgat gtcgtctgaa
     1321 agcccgtcgg acgtgtgttg caacacggtc tgttcttccg ccaactgcgc cagttcgcgc
     1381 tcgatgtgtt cgcggcggat ttgcccttgg ttggtacgcg ctaagagttc ggcggcgcgt
     1441 tgctgcgcct gactgtattg gcgcgtgtgc tgctgttgct gctgcatcag gtttttatgt
     1501 tgcacttcag acgagcgcac ggcagcttcc gcctgtttga acgcggcttc ggcggcggaa
     1561 agttcggggg cgaggttttc cagttcggac gcgatgccgt cgaggcgcgc tttttgggca
     1621 atcaggcttt cctgcgcggg tttggcatag agcaggacgc tgactttatc gacctgatga
     1681 ccttcgggcg tgagccagat ttggtgtgcg cccaaatcgt tttgatgcgc gagggcatag
     1741 ctcaaatcgg gcgcgcacaa tacgccgtcg agccagtagt gcaatgccgt ctgaaacggc
     1801 ggctgcgctt ggatttggtt cagcaatgcc tgtacgggca gggatttttt gatgccgccg
     1861 gagagaccgt ctgaaagcca cgccgcctgc ccctgcggca aaggttcggg cggtacgaaa
     1921 ccttgcggca cggcgcgggc gtgcaggcgt tctgcaagga tgacggacaa ggcgtgctgc
     1981 cactcggcag gcgcggtgat gtgttgccac agctgcggcg cggcggcgtg gtcggttgcc
     2041 tgccagaaat cggctgcttc ctgctgttgc gacaggattt gcgacaacgc ctgctgctgc
     2101 gcctgcaagg tgatatgttg ctgcttcagg ctttggaagc ggtttgaggc cgtctgaaac
     2161 gcctcgcggg cggcgtgtaa ggcttcttcg gcggcgatga tttgttcttc gtaatgctct
     2221 tgccggcttt gcaacaaggc agcggcctcc tgcgcggcgg cggtttccgc ttcgtcgggc
     2281 aggttcaggg cttggttctc ctgtttcaga cggcctttgc gctcttcgtg cttggcaacg
     2341 gtttgttcgg catgggcaag ctgctgctgc ttcaacgcca gttcgcggcg gatgcggttt
     2401 gcctcgtcct gctgggtttg gaaggcggca ttgagcgcgg tttgggtttc ttccaattcg
     2461 ggcagacgct cttcgtgttc ggcaacctgc atcgcccact ccgccagctc ggtttgcttt
     2521 tcttcgacgt ataactcgtt ttcttcaagc tgcacgcgga tttgctgctg ctcttgatga
     2581 atacgctgca actgcgcctg cgccgcctgc ttgtcgcgtt cgatgcgttg gtgcaggttt
     2641 tgctgatggc ggatttgttc ttccaaacgg gcaatctgct cgcgcaacac gccgcgcttg
     2701 ttgctcaatt catgcaccgc ctgttgctgc gactgttcgg cggtctgcaa ggcgtgtact
     2761 tcgtcgttta acgcctgaac ctgcgcggcg gtttcgtcct gctgcgcctg caaagactga
     2821 tgctgctcgg tcgccttgtc ggcggcggca agcgattgct gccattgggc gtaatcgagc
     2881 aaatcctgtt gccggttcaa ctgcgcggtc agggatttgt agcgttcggc ggtttccgcc
     2941 tgtttctcca gcttttccac ctgacgcgcc aattcgttct gcaaatcgcc caaacgctgc
     3001 aaatgctcgc gcgtgtcttt cagacggcct tccgtctcct tgcggcgttc cttatatttg
     3061 gacacacctg ccgcctcctc gatataggcg cgcaattcct ccggccgcgc ttcgatgatg
     3121 cgcgaaatca tcccctgctc gataacggca taaccgcgcg cgcccacgcc cgtacccaaa
     3181 aataaatcgg taatgtcgcg gcggcgcacg gtctgattat tgatgaaata agtcgattcg
     3241 ccctgccgcg tcagctgccg cttgatgctc acctcggcat actgccccca cgcgccctgc
     3301 aaactgtggt cgctgttgtc aaacaccagc tccaccgaag ctcttggcgc aggacggcgc
     3361 gttgccgcac cgttaaaaat cacgtcctgc atactctcgc cgcgaagctg cttcgccgaa
     3421 gcctcgccca acacccagcg caccgcgtca atcacattcg acttgccgca gccgttgggc
     3481 ccgataaccg cgacaagctg ccccggcaca tgaattgtgg tcgggtcggt aaaagatttg
     3541 aagccggaga gtttgatatg ggtcaggcgc ataatggtcg gaaaaaataa aaaagaagcg
     3601 tattttaacg gaaatcccgc cgcaccaccc catatcttgc cggcaaaacc ttaccgcatc
     3661 ccgcaccctc gatgccgtct gaagccttca gacggcattt ttaacgcgcc cgaaccccga
     3721 ttccgccaac gcccgatgct tggcgcggca tttgccgtat tgccccgccg ccgggcgcgt
     3781 tataatccgc cacgacatcc gcccgatttg gaaacctgcc tatgaaaccc gcctatttca
     3841 tctccgacct gcatttgagc gaaaagcacc ccgaactgac cgcgctgttg ctgcgttttt
     3901 tacgttcttc cgccgccggg caggcgcggg cggtttacat tttgggcgat ttgtttgatt
     3961 tttgggtggg cgatgacgaa gtttccgagt tgaatacttc ggttgcgcgt gaaatcagga
     4021 aattgtccga caaaggcgtt gccgtgttct tcgtcagggg caaccgcgac ttcctgattg
     4081 gtcaggattt ttgccggcag gcaggcatga cgctgctgcc ggattactcg gttttggact
     4141 tgttcggctg caaaaccctc atctgccacg gcgacactct gtgtacggat gacagggcat
     4201 accaacgttt ccgcaaaatc gtgcatcgga agcggctgca aaaactgttc ctgatgctgc
     4261 ccctgaagtg gcgcacgcgc cttgccgcca aaatcaggcg cgtcagcaaa atggaaaaac
     4321 aggtcaagcc cgccgatatt atggatgtca atgccgcctt taccgcgcgg caggttcgcg
     4381 cctttaacgc ggaaaggctg atacacggac acacccaccg cgagcatatc catcacgaaa
     4441 acggctttac ccgcatcgtt ttgggcgact ggcataacga ctatgcttca atcctccgcg
     4501 tggacgggga cggcgcggta ttcgtgccgc tggaaaaatg ctgaaaatgc cgtctgaagc
     4561 ctttcgggcg gcttttttta cacgcccctt tcccacactc cctccgccgc atcatcacca
     4621 tccttaaccc gcattaatgg gcgttatcga taaaatgctt gaccgattac ataagattac
     4681 gtaaagtgtg caaacgcata acaattggtc ttaccaattt atcttttaac caaaatatcg
     4741 ggtgtacggg atttgaacgt ctttccaaac ctcgacgcgc cttatgatgc aaatgcctaa
     4801 aaaggattcg ttatggcact tttcctcagc atattcccca tcgtcctgct gatttggctg
     4861 atggtgaaaa aaaacagtat gccctcctac gtcgcgctgc cgattaccgc agtgctgatt
     4921 tacgccatca aacttttcta cttcggcgat gcgggcatgc tgctcaacgc caccgccgct
     4981 tccggcctcg tcaaaacgct cacgccgatt accgtgattt tcggcgcgat tatgttcaac
     5041 cgtatgatgg aaaccacggg ctgcattgat gtcatccgca aatggctggc gaccatcagc
     5101 cccaaccccg tagcgcaact gatgattatc ggctgggctt ttgcctttat gattgaaggc
     5161 gcatccggct tcggcacgcc tgccgcgatt gccgcgccga ttctgatgag cttgggcttc
     5221 aacccgttga aagtggcgat tttcactttg gtgatgaact ccgtccccgt ctctttcggc
     5281 gcggtcggta cgccgacctg gttcggtttc gcaccgctga acctgagtgc cgaagacatc
     5341 ctcgccatcg gcaggcagac cggcgtaatg cacttcttcg caggtttcgt catccccgtc
     5401 atcggcttgg gcttcatcgt accttggtct gaaatccgca aaaacttggg cttcgtcgcc
     5461 attgccgtct tctcctgcac cattccttat gtcgcattgg cgatggtcaa cgaagaattc
     5521 ccgtcgctcg tcgccggcgc aatcggcctg atggtgtccg tattcgccgc caaccaaggc
     5581 tggggcttga gcaaagacca cgccaaagac ccgaatgccg aaaaagtgcc gttcgcccaa
     5641 gtcgccaaag cactcgcccc tttgggtatg ctgatcggca tgctggtggt tacgcgcatc
     5701 aaacagctcg gcatcaaagg cattttgacc agcaaagaag aatggttcag cttccaactg
     5761 ccgtttgatt tgtccaaaat caccgtcagc gactccctga cgattacctt cggcaatatt
     5821 ttcggacaag atgtcagcgc gtcttaccaa acgctgtacg tcccggcttg gattccgttt
     5881 gtgctgaccg tttggatttg catcctgctg tataaaacca aattcaaaga tgcctggacg
     5941 atttattccg taactttcaa tcaaaccaaa aaaccgctgc ttgccctgat gggcgcgctg
     6001 attatggttc agctgatgct ggtcggcggc gacaattcga tggtgaaaat catcggtaag
     6061 gaatttgccg caatggcggg cgaacactgg gtttatttct cgccgtatct gggcgcgatc
     6121 ggtgcgttct tctccggctc caacaccgtg tccaacctga ctttcggccc gattcagcag
     6181 caaatcgcct tggataccgg cctgtccgtt accctgattc tggcgttgca gtccgtcggc
     6241 ggcgcgatgg gcaatatggt gtgcctcaac aacatcatcg ccgtatgtac cgtattggat
     6301 gtgaaaaatt ccgaaggtgc gattatcaag aaaaccgtta tcccgatggc gatttacggc
     6361 gtgattgccg tcgtcgcggc aatgattttc ttcctctagg cgaaacgcca ccatccatag
     6421 cggcagcacg cggggaacgg aaaccgccgt tccccgtctg ggcggcgcgt cgggcagaat
     6481 cccggtgccg gaacagcccg ccccgccaaa caaatgccgt ctgaaaccgg aaaaggcttc
     6541 agacggcatt tttccgccgt ttgcattcag gcgtaatgat gatgcggcat cttgccgtca
     6601 aggcggtagc gcgtgccgca atacgggcag gcaacgctgc ccgattctcc ttcgcacaaa
     6661 ggcaaaaaca ctctcggatg cccgttccac tgctcgttgt cgggtcccga gcaatacagc
     6721 ggcagatttt ccggcaacac ggaaatttcc tgcagattca gattgtccat ttgattttcc
     6781 tttgcgtggt tggcgtgcgc ccattttacg ccatcggcag gctaaaggat attttcggcg
     6841 caaagccgca atccgctata atccccactt ttcagacggc ataccatgac tgcgcttacc
     6901 cttccggaag acatccgcca acaagagcca tccgccctgc tctatcccct cgtttccgcc
     6961 tacctcgaac acaccgccca aaccggcgac gaatccctct cctgcctgtc cgacgaccag
     7021 cacacgctga ccgcattctg ctacctcgac agccaagtcg aagaaggcgg cttcgtgcaa
     7081 ctcatcgcct ccggttacgg cgaatatatt ttccgcaacc cgcttgccga cagcctgcgc
     7141 cgctggaaaa tcaaagccgt gccgaaagtc ttggacaaag ccaaagccct ctacgaacaa
     7201 cacggcaaaa ccatcgaaac gctcgccgac ggaggcgcag acatcgaatc cctgcgcaaa
     7261 cagttccccg actttgaaga atgggacggc gcatactacg aagccgccga acaagacctg
     7321 cccctgcttg cagaacacat acagtcaaac tgggaaacct tcgcccatat cgggcaggcg
     7381 tgattgcgtc tgtttccagc ccgtgtaaaa cagcgtaaaa tcggcaaacc cgaatgacct
     7441 tccgtcccca tcaaggagca agctatgttc ttcaagcaca tcgaagccgc ccccgccgat
     7501 ccgattctcg gtttgggcga agcattcaaa gccgaaaccc gccccgaaaa agtcaacctc
     7561 ggcatcggcg tttataaaga cgcatccggc gcgacacccc tcgtcaaagc cgtcaaagaa
     7621 gccgaaaaac gcctgttgga aagcgaaacc accaaaaact acctcaccat cgacggcgtt
     7681 gccgactaca acgcgcaaac ccaaatcctg ctgttcggca aagaccacga aatcatcgcc
     7741 agccgtcgcg ccaaaacagc gcaaagcctc ggcggtacgg gcgcattgcg tattgcggcc
     7801 gagtttgcca aacgccagtt gaacgcgcaa accatctgga tttccaatcc gacttggccc
     7861 aaccacaacg ccatcgccaa agcggtcggt atccaagaca aaccttatcg ttactatgat
     7921 gccgccaaac acggtttgga ttgggacggc atgattgagg acttgagcca agcgcaaaaa
     7981 ggcgacatcg tcctgctgca cggctgctgc cacaatccta ccggcatcga ccctacgccc
     8041 gaacaatggg aaactttggc aaaactttct gccgaaaaag gctggttgcc gctgtttgac
     8101 tttgcctacc aaggcttcgg caacggtctg gaagaagatg cctacggctt gcgcgtgttc
     8161 ttgaaacaca atacagaatt gctgattgcc agctcttatt ccaaaaactt cggtatgtac
     8221 aacgagcgcg tgggcgcgtt cactttggtg gccgaagatg aagcaaccgc cgcccgcgcc
     8281 cacagccaag tcaaaaccat catccgtacc ttgtattcca atccggcttc acacggcgcg
     8341 aacactattg cgctggtgtt gaaaaatgat gatttgaaag cacaatggat tgccgaactc
     8401 gatgaaatgc gcggccgcat caaagccatg cgccaaaaat ttgtcgagtt gctcaaagcc
     8461 aaaggtgcaa gccaagactt tgatttcatt atcgaacaaa acggtatgtt ctctttcagc
     8521 ggtctgactc ccgaacaagt tgaccgtttg aaaaacgagt ttgccattta tgccgtccgc
     8581 tccggccgca tcaacgttgc cggcattacc gacgacaaca tcgattatct gtgcgaaagc
     8641 atcgtgaaag tactgtaaaa agcacagcca tatgaaaatg ccgtctgaac cttaaccagt
     8701 tcagacggca tctgtttttt gttaaaaaac aataaactat aacaatttca aacaagctaa
     8761 aaacatctat aaaatcaacc caatatgatg aactcaaata aaaatacgaa tatcatcatt
     8821 ctcaaattca aaatttgaac gccacagtat tatccgaagc cacaaaaacc tttccgccgt
     8881 cattcccgcg caggcgggaa tccgggaaac caatgctgca agaattgatt agaaacaact
     8941 gaaaccgagc aaactggatt cccacctgcg cgggaatgac gaaataaggc gagtaaaact
     9001 gatgaaacaa attgattaaa aacaataact tatattattt tatttaaatt ttcgataaaa
     9061 tcaattttcc gtattcaata ctgctccaat caattcccgc gcaccgtcgt ccgccaattt
     9121 cttagcgaca gcgcgtccga gcgcgtcggc atattcggca ggggcttgcg cgtccgcctg
     9181 caacacaacc gaaccgtcgg ggtgtccgac cagtccgcgc aaggtcagca agccgttttc
     9241 ttccgtgcaa tatgcggcca gcggcacttg gcagcttccg cccaaagcgc gcgccaaagc
     9301 tcgttcggcc gtaacgcagg cgtgtgtggt atcgtggttt agcggtttta aaacctcata
     9361 caaatcttcg cggtgcgcgg caatttcgat acccaatgcg ccttgtccgg cggcaggcag
     9421 gctgtcggat tccgacaaaa tcatgcggat gcgtccgtcc aatttcagac gctgcaaacc
     9481 ggcggcggcc aaaataattg cgtcgtattc gccgttatcg agtttggaca aacgggtttg
     9541 cacattgccg cgcaaaggtt tgataagcaa atgcggatag cgcgcgcgca actgggcttc
     9601 gcggcgcagg ctggatgtac cgacgacagc gcctttgggc atttcttcca aacgcgcgta
     9661 ttggttggac acaaacgcgt caaacggatt ggcgcgttcg ccgatggcgg caagcgcgaa
     9721 accttcaggc aaatccatcg gcacgtcctt aatcgaatgc accgccaaat cggcgcgccc
     9781 gtcatataaa gcctgttcca actctttgac aaacaagcct ttaccgccga cttttgacaa
     9841 agttttgtcc aaaatctgat cgccgcgcgt ggtcatgccc aaaatctcga cttcgcaatc
     9901 gggatacaga gccttcagac ggccttggat atgctttgcc tgccacatag caagcaggct
     9961 ttcgcggctg gcgatgacga gttttttcgg gttcatgttc aatgcgttca aagaatggaa
    10021 acggcgtgaa gtctatcata ttttgaacgt ttcttcagac ggcaccgtca ggtctatgtt
    10081 atatttacac ccacaaaaca cgaaacccgc cacaatgaaa cgcaccaaaa aatacccttt
    10141 cttaacgttg cagcggcaac gtttccattt gaactttgaa aacgcctctt ccgccgccgg
    10201 catcccagcc gaacgcgatt tctaccgctg ggcgtggtct gccttgaaaa atgaataccg
    10261 ccgcgccgac atcagcctga ttcttctgga cgaagaagaa gcccgagcct acaaccgcga
    10321 ctaccgcggc aaagattacg ccaccaatgt attgagtttc gcgctcaacg aaggcgaaat
    10381 cctgccctgc caagtttcgg aaaaactgta cggcgatttg attatctgcc cgcaagtggt
    10441 tttgaaagaa gccgccgaac agggcaaaac gcccgaacgg cattttgccc acctgaccat
    10501 acacggcgtt ttgcacctga tgggctacga ccacatcgaa gacgatgaag ccgaaataat
    10561 ggaagccgaa gaaatccgcc tgatgctggc ggcaggcttc cccaacccct accaagagga
    10621 cgaatattaa aatggacggt acgcagtcga aacccaagtt tttcgaacgc ctgatctccc
    10681 gacttgccgg cgagcccgac tccgccgaag acgtattgac cctgctgcgc caagcgcacg
    10741 agcaggaagt ttttgatgcg gatacgcttt taaggttgga aaaagtcctc gatttttccg
    10801 atttggaagt gcgcgacgcg atgattacgc gcagccgtat gaacgtttta aaagaaaacg
    10861 acagcatcga acgcatcacc gcctacgtta tcgataccgc ccattcgcgc ttccccgtca
    10921 tcggtgaaga caaagacgaa gttttgggta ttttgcacgc caaagacctg ctcaaatata
    10981 tgttcaaccc cgagcagttc cacctcaaat cgatattgcg ccctgccgtc ttcgtccccg
    11041 aaggcaaatc gctgaccgcc cttttaaaag agttccgcga acagcgcaac catatggcga
    11101 tcgtcatcga cgaatacggc ggcacgtcgg gtttggtcac ctttgaagac atcatcgagc
    11161 aaatcgtcgg cgaaatcgaa gacgagtttg acgaagacga tagcgccgac aatatccacg
    11221 ccgtttcttc cgaacgctgg cgcatccatg cagctaccga aatcgaagac atcaacacct
    11281 tcttcggcac ggaatacagc agcgaagaag ccgacaccat tggcggcctg gtcattcaag
    11341 agttgggaca tctgcccgtg cgcggcgaaa aagtccttat cggcggtttg cagttcaccg
    11401 tcgcacgcgc cgacaaccgc cgcctgcata cgctgatggc gacccgcgtg aagtaagcac
    11461 cgccgtttct gcacagttta ggatgacggt acgggcgttt tctgtttcaa tccgccccat
    11521 ccgccaaaca taaaaatgcc gtctgaaacc gttgcaagtt tcagacggca tttcgcttat
    11581 ccgcttattt tttgctcaaa gtcagccccg tccaaccgaa taacagggtc aaagccaaac
    11641 tcaaatagca gaaaaaggca tacggcagat attcccaaac cggcacgccc agcgcgtggc
    11701 tgatgaacac gccgcatacg ctccacggta cgagcgggtt aatcaccgtc cccgcatctt
    11761 ccagcgtccg cgacagattg cgggaatgta gtccgagcct gtcgtaaacg ggtttaaacg
    11821 tttctccaga aagcaggatg ctcaaatatt gttcgccaat caggaaatta accccgaccg
    11881 aagtcatggc aacgctgaac gtcgcgcgtc cggcattcgt caagaaggta cggatggcct
    11941 ccaacaggga aggaatcaca ccgagcgcaa acaacagtcc gcccaaactc atcccgagaa
    12001 tcacgatggt ttgcgtgaaa aacatacttt ccaaaccgcc gcgcgaaatc agtttgacaa
    12061 catctttaaa cgcttcgcct tcgagtttgt agccgccgta aaaccacgca ccgagctgac
    12121 gcagatcggg cgtgctgtgc agatacgtta cagcaacggc aaccatgacg gtaaagagca
    12181 tggcgacgac ggcgttgatg cgcatcaatg ccaaaatgac caacagcgca aacggaatca
    12241 gcgaatagcc gtgcaccaat cccgtggctt caagctggct gcggaaggat tcgacgctgt
    12301 tcaaatcctg cgcggcgaca ttcggcaaaa gccaaagcat cagtgccgca ctaatgagcc
    12361 acgcggggat ggtggtgtac atcatatttt tgatgtgctc aaacaagtcg atgccgacga
    12421 tggacgcgga aatacccgtc gtatccgaaa gcggggacat tttgtcgcca aaaaatgcgc
    12481 ccgaaacaat cgcgcccgcc gtcatcgcca tatcggcctg aaacgccgcc gccatcccca
    12541 taaaggcaac gccgacagtg gcgcaggtgg tcaggctgct gccgatggac acgccgatga
    12601 cggaacacag cgcgaaggcg gaaaaataaa aataagtcgg agaaatcagc ccgaaaccgt
    12661 aatacatcag cgtcggaatc gcaccgctca tcatcagtgc gctgaccatc agcccgatga
    12721 agaaaaacag gtaaatcgcg cccataccct gattcaacgc gcctatcatg ccctgctgca
    12781 tatcgttgta tttcaaaccg cgcgccaagc cgtacaaaat cagcacgacg atggcggcaa
    12841 taatggacat atgcggcagc cactccaatg aaatgatggt atagcccatc gcggcaatca
    12901 gagcgacgac gacggcaagt gcctcaccgc gcggcatatc gagtaaggat ttgaaagcaa
    12961 acatgattct ttgcccgatt tgatataaag ttaacggatt ctaacacact ataccgaatc
    13021 cgcccgacgt tttcagacgg cattttagcg ttttacaaaa gccggacaga aacaaaacgg
    13081 caattttctc aatttaaatt aatttttttg aaatataatt ttttattttt ttgtttgttg
    13141 gcatataaga cttttatgga ttgaaatatt aaaacctcgg cacacacgcc gcaaacaccg
    13201 gtacgcaaaa atgccgtctg aaagatgttc agacgacatt tctcaagaaa aagattattt
    13261 gtccgcccta tatccgtaca ccgcaaaaaa cacgatatac agataacaga tgacggaaac
    13321 gacaaacgaa gacatcaggg tgtaagtatc tgccacccag ccctgcacga caggaacgac
    13381 cgcaccgccg acaatcgcgg tacacagtac accggaagcc gcgttggtaa attttcccaa
    13441 tcctttggtt gccaaagaga aaatcgtcgg gaacataatc gagttgaaaa aaccgatggc
    13501 aagcagcgac cacatcgcca catccgcatt gccgctaccc gtcgccatcg cgatggcaag
    13561 cagtacgacc gcagccgatg cgttaaacgc caaataacgg ttgggcgcga atttcgccat
    13621 caccgccgaa ccgaggaaac gtccgaccat cgcgccgccc caatagaacg acaggtaatg
    13681 cgcggcagaa gcatgatcca gccctttcag ataacccaat acgttgacca tcaacgaacc
    13741 gatagacacc tccgcgccga catagcagaa aatgcctgcc gtaccgaaca cgagatgttt
    13801 gtattgccat acgccggttt tgccgtcgtg gttgtgcgcg ctttcctcgg cggcaatttt
    13861 gcgcgcgtcg ggcagccgga tcattttcac gaaaacggca aggataatca gcagccccgc
    13921 cagtcccaaa tagggaatct gtacggaaga aatctgttcc gccttgctga cggtttgggt
    13981 tgcgtccgcc aaaatcagga acgcgccgat ttgcggcgca atggtcgtac ccaaagcgtt
    14041 aaacgcctga accagcgtca gtgttgccga ttccttgccg ggtttcgcca gcagggtaac
    14101 ataaggatta ccggcaacct gaagcagcgt tacgccggaa gccaaaataa acaacgcgcc
    14161 caaaaatacc gcgtaagaat ggctgcccgc agcaggataa aacagcaggc atccgaccgc
    14221 cgtcagcaga aagccgccga taacgccgtt tttgtagccg actttgccga caaaagcccc
    14281 catcgggatg gacatcaccg cataggcggt aaagaaacag aattggatca gcatcgcctg
    14341 aacgtaagac aggtcgaaaa tttctttcaa atgagggata aggatgtcgt tcatgcaggt
    14401 aataaaaccc atcatgaaga acagcgtggt caagacgacc aatggggacg tatgattgtt
    14461 ttgtgattgc gcagacatga tttgcctttc gtggtgtcgg aatattaccc gtttcaagaa
    14521 aatagaaatt gacatttctt aacggcaatg caaatcatac tgatatttga acattttgca
    14581 aactttatcg gatgcaggca atgcgccgat ctgaaattca agcagcagaa attaatattt
    14641 tacagttgct tgcatagatt ttaatagatt ttaaaaaatt ccggcggact tgcaaaaacc
    14701 gttcccaata aaatgaaaat ccgccgccgt atccggaaaa ttcggaaatg ccgcatcgtg
    14761 gcatcaaaac cggagcggaa agaatgtcgt atgcccgaca aaaaaggggc atacggctgt
    14821 gccgtatttt ttgcaccgtc ctccgccctt cggcgggggc gtgaaaatat agtggattaa
    14881 caaaaatcag gacaaggcga cg
//
LOCUS       DAWEWP010000038        14718 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWEWP010000038 DAWEWP010000000
VERSION     DAWEWP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.038"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(24..1067)
                     /gene="galE"
                     /locus_tag="WGN53_000843"
                     /pseudo
     CDS             complement(24..1067)
                     /gene="galE"
                     /locus_tag="WGN53_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(1060..2058)
                     /gene="dcm"
                     /locus_tag="WGN53_000844"
     CDS             complement(1060..2058)
                     /gene="dcm"
                     /locus_tag="WGN53_000844"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0139857.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(2089..4362)
                     /locus_tag="WGN53_000845"
     CDS             complement(2089..4362)
                     /locus_tag="WGN53_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ0139858.1"
                     /translation="MNITQILSQELSATTAQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDGLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETESEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4427..5077)
                     /locus_tag="WGN53_000846"
     CDS             complement(4427..5077)
                     /locus_tag="WGN53_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0139859.1"
                     /translation="MISVEHVSKQYQMRGGMRTVLDDINFSLQKGEKVGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMHQFEDMDKAYEYYNSLP"
     gene            complement(5074..5871)
                     /locus_tag="WGN53_000847"
     CDS             complement(5074..5871)
                     /locus_tag="WGN53_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0139860.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(5871..7034)
                     /gene="ctrB"
                     /locus_tag="WGN53_000848"
     CDS             complement(5871..7034)
                     /gene="ctrB"
                     /locus_tag="WGN53_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ0139861.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7049..8224)
                     /gene="ctrA"
                     /locus_tag="WGN53_000849"
     CDS             complement(7049..8224)
                     /gene="ctrA"
                     /locus_tag="WGN53_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ0139862.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8359..9492
                     /gene="neuC"
                     /locus_tag="WGN53_000850"
     CDS             8359..9492
                     /gene="neuC"
                     /locus_tag="WGN53_000850"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ0139863.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALEVSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            9496..10182
                     /gene="neuA"
                     /locus_tag="WGN53_000851"
     CDS             9496..10182
                     /gene="neuA"
                     /locus_tag="WGN53_000851"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ0139864.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10183..11232
                     /gene="siaC"
                     /locus_tag="WGN53_000852"
     CDS             10183..11232
                     /gene="siaC"
                     /locus_tag="WGN53_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ0139865.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIS"
     gene            11384..14497
                     /locus_tag="WGN53_000853"
     CDS             11384..14497
                     /locus_tag="WGN53_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0139866.1"
                     /translation="MAVIIFVNGIRAVNGLVKSSINTANAFAEEGLDVHLINFVGNIT
                     GAEHLYPPFHLHPNVKTSSIIDLFNDIPENVSCRNTPFYSIHQQFFKAEYSAHYKHVL
                     MKIESLLSAEDSIIFTHPLQLEMYRLANNDIKSKAKLIVQIHGNYMEEIHNYEILARN
                     IDYVDYLQTVSDEMLEEMHSHFKIKKDKLVFIPNITYPISLEKKEADFFIKDNEDIDN
                     AQKFKRISIVGSIQPRKNQLDAIKIINKIKNENYILQIYGKSINKDYFELIKKYIKDN
                     KLQNRILFKGESSEQEIYENTDILIMTSESEGFPYIFMEGMVYDIPIVVYDFKYGAND
                     YSNYNENGCVFKTGDISGMAKKIIELLNNPEKYKELVQYNHNRFLKEYAKDVVMAKYF
                     TILPRSFNNVSLSSAFSRKELDEFQNITFSIEDSNDLAHIWNFELTNPAQNMNFFALV
                     GKRKFPMDAHIQGTQCTIKIAHKKTGNLLSLLLKKRNQLNLSRGYTLIAEDNSYEKYI
                     GAISNKGNFEIIANKKSSLVTINKSTLELHEIPHELHQNKLLIALPNMQTPLKITDDN
                     LIPIQASIKLEKIGNTYYPCFLPSGIFNNICLDYGEESKIINFSKYSYKYIYDSIRHI
                     EQHTDISDIIVCNVYSWELIRASVIESLMEFTGKWEKHFQTSPKIDYRFDHEGKRSMD
                     DVFSEETFIMEFPRKNGIDKKTAAFQNIPNSIVMEYPQTNGYSMRSHSLKSNVVAAKH
                     FLEKLNKIKVDIKFKKHDLANIKKMNRIIYEHLGININIEAFLKPRLEKFKREEKYFH
                     DFFKRNNFKEVIFPSTYWNPGIICAAHKQGIKVSDIQYAAITPYHPAYFKSPKSHYVA
                     DKLFLWSEYWNHELLPNPTREIGSGAAYWYALDDVRFSEKLNYDYIFLSQSRISSRLL
                     SFAIEFALKNPQLQLLFSKHPDENIDLKNRIIPDNLIISTESSIQGINESRVAVGVYS
                     TSLFEALACGKQTFVVKYPGYEIMSNEIDSGLFFAVETPEEMLEKTSPNWVAVADIEN
                     QFFGQEK"
BASE COUNT         4370 a         3312 c         2922 g         4114 t
ORIGIN      
        1 gtttttttgt ttgcggtttg catttaatcc ccatatccgt tggggttgcg gctgacccaa
       61 cgccacgaat cttccatcat ttgctgcaag ccgcgtttgg tttcccagcc ggtttgttgt
      121 ttggtatggg acgggtcggc aaaggaacac gccaaatcac cggcgcggcg cggtttgatt
      181 tcaaatggaa tggttaaacc cgaagccgct tcaaatgcgc ggatgatttc caataccgaa
      241 gaagcgcggc cggagcctaa gttcagcaaa tgcgtgcctg ctacattact ttttgcctgc
      301 atagccgcga catggccttc tgccaaatcc atcacatgaa tatagtcacg catccccgtg
      361 ccgtcggggg tagggtagtc atcgccaaat accgccaatt gcggcagttt gcctgccgcc
      421 acttggcaga tataaggcaa caaattattc gggatgccgt ttggctgctc gccaatcaaa
      481 ccactttcgt gcgccccaat cgggttgaaa tagcgcaaca aaatcacgct ccaacgcgga
      541 tcggcctttt ggatgtcggt taacatccgt tccaccatcg ctttggatgc gccataagga
      601 ttagcggtat cgcccgggcg catatcttcc gtatagggca ttttacgtat catggcgcga
      661 cgcattaaag taaacgccaa agcgcgttta tactggttat ccagtttccc tatattttta
      721 cgttacaaat caagttgttg gtattcatcg tgaaaataga atttttcggc ataacgctga
      781 ctcataaaac cggcatgcgg gctacctgaa aaaatcattg cgccatcatc gtcatttaat
      841 gtttcatggt tgttttctat taatgctaaa gcaatttggt aattaattgc caaaatatcg
      901 ttagtaatca cacgatagcc gcgttttttg gcttcatagg caaaggaaca gcctccacta
      961 aatacatctg caactgtatc tacatcagac ggaagctggt cacaaatcca ggaagctatt
     1021 ttctctttat taccgatata gttaattttc ggatattgct tattcatttt cactcatttt
     1081 taaaatactt tcagcaatag cttttgccaa taaaggcggt acggcgttac caacctgctg
     1141 ctgggcaatt ttgctgccgc aaaaaataaa attatcaggg aaagattgta aggcagctaa
     1201 ttcacgaacg gttaaagccc gattctgttc atagtgaaaa actttgcgca tatctcctgt
     1261 aatacaaacg gctggtttgt tgctgttgta acggatgtat ttacggatat cacctgtttt
     1321 cggacgtaat ggttcaggaa tatcgttacg gttacctcca tttttaacaa atgccatttt
     1381 ttctaacatt tgtgccgaat gattcatagc ttcatgattt gcaacgtgtg gattgctttc
     1441 gccagcagcc agttttggaa aatgtcctat tgctgatcca acagtctgat gggaaatctg
     1501 caaaggttcg ggaaaggaaa ttttgccttt atccctcctc ccgataaata tcactcggct
     1561 acgtatctga ggaacaccga aatcggctgc actcagtatc ttacattcca ccgaataacc
     1621 gatattctga aatgcttgaa taatctcaat acgtgtttta cctgaattgt gtgtatagag
     1681 tcgcgctaca ttttccataa caaaaaaata tggttggaca attttaacta ttcggacaaa
     1741 ctctttaaat aaatggttgc gtgggtcatc tgtaaatgtc cgtccaatct ttcctgccat
     1801 actaaaacct tgacaaggtg gtcctccaat aatcaaatca actgcttgtc cgttaagaca
     1861 attgattaaa tcttgttcgg ttagtgtggt taaatctttt tgcagtaatt gatgatgggg
     1921 gaagttggta cggtaagtct gacaataatc agactccatt tcaacagaaa gcaattgttg
     1981 gaatccggct tgttcaaaac ccaaggatag gcctcctgct cctgagaaaa ggtcaatata
     2041 ggtaagtggt gtgtgcataa gtcaaaatcc ataaactctt cgattatttt accgcttcag
     2101 cttcgcaaac gcatccgcca tcgccgaatt ggctggggcg cggtcgttgc gttggggttt
     2161 gcggccggct gtccgttcgc ggctgcggtt ttcagacggc attttatgtt ttgcgccgcc
     2221 cggttcgtca tccaagcgca tggtcagcgc gatgcgtttg cgtgcagcat cgacttccag
     2281 cactttcact ttcaccacgt cgccagcttt caccacttcg cgcgggtctt ggacgaactt
     2341 gttggacagg gcggagatgt gcaccaagcc gtcctgatgg acgccgatgt ccacgaacgc
     2401 gccgaagttg gcgacgttgg aaaccacgcc ttcgagtatc ataccgactt gcaagtcgct
     2461 gatttcgtgg ataccttcgg caaacgatgc cgtctgaaac tcgccgcgcg gatcacggcc
     2521 gggtttttcc agttcggaca ggatgtccaa aatggtcggc aggccgaagc gttcgtcggt
     2581 gaagtcggac gctttgattt gcttcacgcg ctcgcggttg ccgatgagtt cggcggcgct
     2641 aatgccttgt tgcgccagca ttttggcgac gacgggatag gcttcggggt ggacggcgct
     2701 cgcgtccaac ggctctttac cgccgttaat ccgcaaaaag cctgccgcct gctcgaaggt
     2761 tttttcgccc aaacgcggta ctttcagcaa ttttttgcgg ctgtcgaacg cgccgttttc
     2821 atcgcggtag gcaacgatgt tttgggcaag ggtttgattc aaaccggaaa ttcgcgccaa
     2881 gagcggggcg gaggcggtgt tcacgtccac gccgacggcg ttcacgcagt cttcgaccac
     2941 cgcgtccagc gatttggcga gctggctttg gttcacgtcg tgctgatact gccccacgcc
     3001 gatggatttg gggtcgattt tgaccaactc ggcaagcggg tcttgtagcc tgcgggcgat
     3061 ggacaccgcg ccgcgcaggg aaacgtccaa gtcggggaac tcgcgcgccg ccagttcgga
     3121 cgcggaataa atcgacgcgc cggcttcgga cacgacgatt ttatgcagcc ccattttcga
     3181 catttcacgc accagttcgc ccgcgatttt gtcggtttca cggctggcgg tgccgttacc
     3241 gatggcgatg agcttcacgc cgtgctgttt aatcaggcgc gacagcgttg ccaacatatt
     3301 gttttcttga tgcaaataga cgatgacggt atccagcagc ttgccggtgt cgtccaccac
     3361 ggcgcatttc acgccgttgc ggtagccggg gtcgagaccc agcgtggtca gccgtccggc
     3421 gggcgcagca agcagcaagt ctttgagatt gcgggcgaac acggtaatcg cgtcggtgtc
     3481 ggcggcttct ttcaaacgat tgagagcttc aagttccaac gacaaaaaga ttttcgcgcg
     3541 ccaagtcagg cgcacggtgt cgcgcagcca tttgtggccg tctgaaactt tgaagcggcg
     3601 ggcgatgatt tgctcgtatt cgctttgctg cgtaatcggc gtgtcgtcgg gctgatattt
     3661 gagcgcgatg ttcaacacgc cttcgttgcg tccgcgcaaa accgctagcg cacgatggct
     3721 gggcatcgca cgaataggtt cgcggtggtc aaaataatcg ctgaattttt cgctttcggt
     3781 ttctttgcct ccaacaactt gcgcgtggat ttcggcttcg ttccacagct tgtcgcgcag
     3841 cgtgccgatg agttccgcgt cttcggcaaa ctgctccatc agaatcgcac gcgcgccgtc
     3901 caacgcggct ttggcatcgg ggacgttttc gttcaggtag ccttgtgcgg cggcttccac
     3961 gtcctgcgac tgctcggcaa gcaacacgtc cgccagcggc tgcaaaccgt gttcgcgcgc
     4021 aatttgcgcc ttggtgcggc gtttgggctt gtagggcaga tacaggtctt ccagcgcggt
     4081 tttgttgtcg gcggcttcga tttgcgcgcg gaggccgtct gaaagtttgc cttgctcttc
     4141 aatgcttttt aacacaacgg ctttgcgctc ttccaactcg cgcaggtatt gcagccgctc
     4201 ggcaagccgg cgcagctgcg tgtcgtccag cccgccggtg gcttctttac ggtaacgcgc
     4261 gataaacggc acggttgcgc cgtcgtctaa aagctcaata gcggcattga tttgcgcggt
     4321 agtcgcggaa agttcttggg aaagaatttg agtaatgttc atcaatagaa ttccaacgga
     4381 caggccgtct gaaatttcag acggcctgat ttaaaaaaca atcgctttaa ggcagcgaat
     4441 tataatattc gtaggctttg tccatatctt caaactggtg catatgccct ttttccagca
     4501 ccatcgcatt atcgcaatat tgcttcatgg cgctgtggct gtgcgacacc aagatgatgg
     4561 aacggtcttt gcgcttttca aacaactcgt acttacattt atcggcaaag cgcgagtcac
     4621 caactgcaat cacttcgtca atcaaatagc aatcaaactc taccgccaac gacaaagcaa
     4681 atgccaaacg cgccttcata cctgaagaat aacgcttgac cggctcgtaa agatattgcc
     4741 ccaactcgga aaactcttcg gtaaagttct tcacataatc gatatcgaca ttgtaaatcc
     4801 ggcagatgaa acgcaaattg tccataccgg tcagactacc ttgaaacgca ccggagaatg
     4861 ccaaaggcca agaaatactc attgtccgct tgatttcacc cgtggtcggc ggctcgacac
     4921 cactaatcaa acggatcaac gtcgatttac ctgcaccgtt tcgacccaaa ataccaactt
     4981 tctcaccctt ttgcagcgaa aaattgatat cgtctaacac cgtccgcata ccgccgcgca
     5041 tttgatactg cttggaaaca tgttcaaccg aaatcattgc ggctcgactc ctttactgaa
     5101 tttactgacc atcgccaagc caaacaacaa caacaccaga ttgcacaata cgatatacca
     5161 aggattttca taggtaatta catcgctgcc aaaatatccg gcacggaaca tttctgtgcc
     5221 atgcaccatc ggaatcatta atgcatattc ttgtaccttg ggcggcaaat tatgcacaaa
     5281 aaagaacgca ccggataacg gcatcatcac aaaagtcaat gtgccccaaa tcttgccaaa
     5341 cggctcgaaa ttaaaggcaa tcgaacaaat caccaaaccc aaaccaatcg caaaaaaagc
     5401 catcaaaagc caagccatca gcatataaaa catatctgcc ggcatttcaa tccagccaat
     5461 cgcaatcaat accgccataa tcacaatctg cgcaatggtt gcaccagcaa tttccaaaat
     5521 catgcgcgcc aagatggtat ccaaaactct tacattgcgg tgataaagca agctggcatt
     5581 tgaagaaatc gacccaactg cccgttttga ggcattacgc cacatcatca acatcggata
     5641 gccagtaatc gcaaatgcga caatattcaa agttgaatat cggtctgccc ttaaaaattt
     5701 ccacatcaag acgataacga atgtcatcag caacggctca acaaacagcc ataaaaagcc
     5761 aatattattg cgaccgtaac gggtgataat ttcccgcatc aacagcgcac cgattacgcg
     5821 cctttgaatg gctaaagatt cccaaaatga tgttttatgc aaggctttca tcagttttta
     5881 tgctcacgaa tgctggcagt caacaggctc aaaataccat aaaccatcaa gccgataatc
     5941 agagtggcaa caatgttgta taaccgttta ggctcatgtg ccaaatccgg caggctcggt
     6001 tgcgagatca cttccaaata aagctgctga cggtctgctt caaccttggc actttccaaa
     6061 gaagtcatgg cggctgccaa ctgctgctct gccaactggt tttccaaata cacacgctga
     6121 tattcggcag cctgattaga caacgaagaa tgcccaccgc ccgaaatggc acgtaactgt
     6181 tggtcaattt ctttacgcaa gctctgctca cgcgcctgca aacccggaat ctgcggattc
     6241 tccggagtga ctgctttcac ctgatccagc tgggtttgaa tcacaatcaa ttcatcttgc
     6301 agcttggaaa ccaaccccat ttgcacttcc gattgcgctt tcaaatcaaa aacgccattg
     6361 gcaatccggt aatccgtcag attctgagag gcttccttta cccgctctgc cgccgttttc
     6421 actacttctt ccgcatagcg caccgtatca gcacgtgcac gatcgttcaa ctggttaatc
     6481 aatgcttcac cttgttttaa caaagcctca ttgattttct tagattccag cgcatcaaag
     6541 gaagttacat tcaacgtgga aatacccgaa accgtatcaa aattgatcat cacctgattt
     6601 ttatagtatt gataaaaagc ctcttcctcg ccacggaacc caaacccatt aaagcggctg
     6661 aacgcatcac ctttggtttc ataaaactca cgcaccggca agattttacg cagttcatcc
     6721 aaagacgagc gcgaacgcat atactcccca accgtgtaaa tatcatcttg cgcacgggca
     6781 aaacctgtgc cctgcaaaat ggcacccagg ccattgagag aagattggct tttaggcgag
     6841 cgcaccacaa agctcgattg cgacgtaaaa cgatcggaag cgaagaagcc gaaatacacc
     6901 aacgaaatta ccgtagggat aatcaccgtt acccaaaata aagggcttag ctttttaatc
     6961 caacttttct ttttcggctt tttacgctcg gctttggttt cagtggcaac tgccacagga
     7021 agttgctcag acatcttact tactcacatt aattagttaa attattaata ctgttcgcgc
     7081 cactggtaac cggcgagaac acaaacgaca aaaatttctg cacttcagcc aacggcgcat
     7141 tcgacacata caatacatct ttattcttca caggaaagcg ctgcatagaa aatagcgaat
     7201 gcgcatcagc catattcaca cgatataccg ttggaatctc tgcctcactg ccataacctt
     7261 gagcaatcca tttatcctga cgttctgccg gcaattccac caatggcgta tagcggaaca
     7321 caaacacacc acgcgcatca gaacggcgat cttgcaaacc gcccatacgg ccaatggctt
     7381 cagaaagcga taagcctctg gctgaaaaac cgatttcttg tgttctcccc accgcaccca
     7441 tagacgtaaa ggtataggga ttggtaatca tggtaaccac atcaccgcga cgcagcaaaa
     7501 tattttgtcg cggatttgca actaaatctt ccaaggcaac agttcgtact acattgccac
     7561 gtgtcagctg cacattcgta tcctgcacat ttgccgttga accacctacc gcagccaccg
     7621 catccaacac acgctcaccg gctgccgtca gcggcatacg cacactattc ccagcacgaa
     7681 tcaccgacac attcgccgca ttattctgca ccaaacgcac catcacttgt ggctgattgg
     7741 ccattttttt caggcggcct ttaataattt cctgaacctg accaggcgtt ttaccgacca
     7801 ccgaaatatc gccaacaaac ggcacagaaa ccgtaccacg tgccgtgacc aactgctctg
     7861 gcaacttagt ttgatgcgca ctacccgagc ccatcgaaga aaggccacca ccaaacaata
     7921 ctgccggcgg cgcttcccaa atcataatat ccaatacatc accaatattt agcgtaccag
     7981 ccgaagcata accatcgcca aactgagtga atgactgatt tatctgagcc ttatataata
     8041 actgagcaac cgtatgattc acatcaatca gctccacttc aggaatttga acttcagatt
     8101 gttgccctaa agagacaatt ttttttgcgc tggggcctga tgaaggaatc gcagagcatc
     8161 ctacaattaa acttccacac aataataata ctgcgtgacg aatataaaat ttcactttaa
     8221 acacaagcca aatcctaata taattataaa tggcctaatt atagcactta atcgaaataa
     8281 atttatgagt acgtagagta taattagtat tcttctttcc aacttcctta tacttatata
     8341 cttatagatt ctaaaatcat gaaaagaatt ctttgcatta caggtaccag agccgacttc
     8401 ggcaagctaa aacctttatt agcctatatt gaaaatcacc cagaccttga attgcatttg
     8461 attgtaactg gtatgcatat gatgaaaaca tatggcagaa cctgcaagga agtaactcga
     8521 gaaaactatc aacatacata tctgttttca aatcaaatcc aaggtgaacc aatgggtgcc
     8581 gttttaggca ataccattac gtttatctct cgtctatctg atgaaattga acctgatatg
     8641 gtcatgattc acggcgaccg tttagaagca ctagcaggcg caactgtagg tgcattaagc
     8701 agccgtttag tttgccatat cgaaggtggt gaactatctg gtacagtaga tgactccatt
     8761 cgtcattcta ttagtaaact ttctcatatc cacttggtag caaatgaaca agctgtcact
     8821 cgcctagtgc aaatgggaga aaaaagaaag catattcaca tcatcggctc ccccgattta
     8881 gatgttatgg cctcttccac cctcccatcc ttagaagaag tcaaagaata ttacggttta
     8941 ccatacgaaa attatggtat ttctatgttt caccccgtga ctacagaagc acatttaatg
     9001 ccacaatatg cggcccaata tttcaaagca ttagaagtaa gtggccaaaa tatcattagc
     9061 atctacccta ataatgacac tggcactgaa agtattctgc aagaattatt aaaatatcaa
     9121 tctgataaat ttattgcatt tccttccatt cgatttgaat actttttagt cttattgaaa
     9181 catgctaaat ttatggtcgg caactcaagt gcaggtatta gagaagctcc tctctacggt
     9241 gtcccttcaa ttgatgttgg tacacgccaa agtaaccgcc atatgggaaa atctattatt
     9301 catacagatt atgaaactaa aaatatcttt gatgcgattc aacaagcatg cagtttaggc
     9361 aaatttgaag cagatgatac ctttaatggc ggagatactc gcaccagcac agaaagattt
     9421 gctgaagtaa tcaacaatcc tgaaacgtgg aatgtttctg ctcaaaaacg ttttatcgat
     9481 ttgaatcttt aaattatgga aaaacaaaat attgcggtta tacttgcgcg ccaaaactcc
     9541 aaaggattgc cattaaaaaa tctccggaaa atgaatggca tatcattact tggtcataca
     9601 attaatgctg ctatatcatc aaagtgtttt gaccgcataa ttgtttcgac tgatggcggg
     9661 ttaattgcag aagaagctaa aaatttcggt gtcgaagtcg tcctacgccc tgcagagctg
     9721 gcctccgata cagccagctc tatttcaggt gtaatacatg ctttagaaac aattggcagt
     9781 aattccggca cagtaaccct attacaacca accagtccat tacgcacagg ggctcatatt
     9841 cgtgaagctt tttctctatt tgatgagaaa ataaaaggat ccgttgtctc tgcatgccca
     9901 atggagcatc atccactaaa aaccctgctt caaatcaata atggcgaata tgcccccatg
     9961 cgccatctaa gcgatttgga gcagcctcgc caacaattac ctcaggcatt taggcctaat
    10021 ggtgcaattt acattaatga tactgcttca ctaattgcaa ataattgttt ttttatcgcc
    10081 ccaaccaaac tttatattat gtctcatcaa gactctatcg atattgatac tgagcttgat
    10141 ttacaacagg cagaaaacat tcttaatcac aaggaaagct aaatgcaaaa caacaacgaa
    10201 tttaaaattg gtaatcgttc agtaggttac aaccacgaac cattgattat ctgtgaaatc
    10261 ggcatcaatc atgaaggctc tttaaaaaca gcttttgaaa tggttgatgc tgcctataat
    10321 gcaggcgctg aagttgttaa acatcaaaca cacatcgttg aagacgaaat gtctgatgag
    10381 gccaaacaag tcattccagg caatgcagat gtctctattt atgaaattat ggaacgttgc
    10441 gccctgaatg aagaagatga gattaaatta aaagaatacg tagagagtaa gggtatgatt
    10501 tttatcagta ctcctttctc tcgtgcagct gctttacgat tacaacgtat ggatattcca
    10561 gcatataaaa tcggctctgg cgaatgtaat aactacccat taattaaact ggtggcctct
    10621 tttggtaagc ctattattct ctctaccggc atgaattcta ttgaaagcat caaaaagtcg
    10681 gtagaaatta ttcgagaagc aggggtacct tatgctttgc ttcactgtac caacatctac
    10741 ccaacccctt acgaagatgt tcgattgggt ggtatgaacg atttatctga agcctttcca
    10801 gacgcaatca ttggcctgtc tgaccatacc ttagataact atgcttgctt aggagcagta
    10861 gctttaggcg gttcgatttt agagcgtcac tttactgacc gcatggatcg cccaggtccg
    10921 gatattgtat gctctatgaa tccggatact tttaaagagc tcaagcaagg cgctcatgct
    10981 ttaaaattgg cacgcggcgg caaaaaagac acgattatcg cgggagaaaa gccaactaaa
    11041 gatttcgcct ttgcatctgt cgtagcagat aaagacatta aaaaaggaga actgttgtcc
    11101 ggagataacc tatgggttaa acgcccaggc aatggagact tcagcgtcaa cgaatatgaa
    11161 acattatttg gtaaggtcgc tgcttgcaat attcgcaaag gtgctcaaat caaaaaaact
    11221 gatattagtt aataataaaa tagattaagc tattcttaaa ttcagaatat tgcttatcta
    11281 tattaaaaat ttctaatttt taaggttctg attgaaatca gaaccttatt tcaactatta
    11341 ctttttactc ataatcgaat tatatacttt aggactttat aatatggctg ttattatatt
    11401 tgttaacgga attcgggctg taaatggcct tgttaaatca tctatcaata ctgcaaacgc
    11461 ttttgctgaa gaaggactgg atgttcattt aattaatttt gttggcaata ttactggagc
    11521 agagcattta taccccccat tccacttaca tcccaatgtc aaaacctcca gcatcataga
    11581 tttatttaat gacattccag aaaatgttag ctgccgaaat actccttttt attctattca
    11641 tcaacaattc ttcaaagctg aatatagtgc ccactataag catgttttga tgaaaattga
    11701 atctttatta tctgcagaag atagcattat cttcactcat cctcttcaac tggaaatgta
    11761 tcgtttagcg aataatgata tcaagtcaaa agccaaacta attgtacaaa ttcatggtaa
    11821 ttatatggaa gaaatccata actatgaaat tttggcacga aatatcgatt atgttgacta
    11881 tcttcaaacg gtatctgatg aaatgctgga agaaatgcat tcccatttca aaatcaaaaa
    11941 agacaaatta gtttttattc caaacatcac ttatcccatt tcattagaaa aaaaagaagc
    12001 tgatttcttt attaaggata atgaagacat cgataatgct cagaaattta aacgtatctc
    12061 tattgttggc agcattcagc caagaaaaaa ccaattggat gccattaaaa tcatcaataa
    12121 aattaaaaat gaaaattaca ttttacagat atatggcaaa tctattaata aagattactt
    12181 tgaattaatt aaaaaatata ttaaagacaa taagttacaa aaccgtatct tattcaaagg
    12241 tgaatcttcc gagcaggaaa tttatgaaaa tacagatatc ctgatcatga catcagaaag
    12301 tgagggattt ccatatatat ttatggaagg catggtgtat gatattccaa tcgttgtata
    12361 tgattttaaa tatggagcga atgattacag taactataat gaaaatggtt gtgtttttaa
    12421 aactggtgat atttctggaa tggcaaaaaa aataattgag ctattaaata acccagaaaa
    12481 atataaagaa ttagttcaat ataatcacaa tcgcttctta aaagaatatg caaaagatgt
    12541 ggttatggct aaatatttca ctattcttcc gcgcagcttt aataacgtat cattatcgtc
    12601 tgctttcagc cgaaaagaat tggacgaatt ccaaaatatt actttttcta ttgaagattc
    12661 taatgattta gctcatattt ggaatttcga gctaaccaat cctgcacaaa atatgaattt
    12721 ttttgcttta gttggcaagc gaaaatttcc aatggatgct catatccaag gaacacagtg
    12781 tacgattaag atagctcata aaaagacagg gaatttattg tcgcttttac taaaaaaacg
    12841 aaatcagttg aatttatcaa ggggatatac cttaattgca gaagataata gctatgaaaa
    12901 atatattgga gcaatatcta ataaaggtaa ctttgaaatt attgcaaata aaaagagctc
    12961 attagttact ataaacaaaa gtaccttaga gttgcatgag attccccatg aactacatca
    13021 gaataaatta ctgattgctt tacccaacat gcaaacgcct ctaaaaatta ctgatgataa
    13081 tttaatacct atccaagcct ccataaaatt agaaaagatt ggaaatactt attacccatg
    13141 tttcttgcca tctggcatat ttaataatat ctgcttagat tacggtgaag aatccaaaat
    13201 tattaatttt agtaaatatt cttataaata tatctatgac tcaattcgtc atattgagca
    13261 acatacagat atatcggata ttatcgtttg caatgtttat tcttgggaac ttattcgtgc
    13321 ctcagttatt gagagcctta tggaatttac cggaaaatgg gaaaaacact ttcagacttc
    13381 tcctaaaatt gattatcgat ttgatcatga aggtaagcgt tcgatggatg atgtcttttc
    13441 agaagaaaca tttattatgg aatttccgcg taaaaatggt atagataaga aaacagcagc
    13501 cttccaaaat ataccaaaca gtattgtaat ggagtatccg cagaccaatg gttacagtat
    13561 gcgcagtcat tcactgaaaa gtaatgtagt tgcggcaaaa cattttcttg aaaaattaaa
    13621 taaaattaag gtagatatta aatttaaaaa gcatgacctt gcaaacatca aaaaaatgaa
    13681 tcgaattatt tatgagcatt taggcattaa cataaatatc gaagcatttc taaaaccacg
    13741 attagaaaaa tttaagcgtg aagaaaaata ttttcatgat ttcttcaaaa gaaataattt
    13801 taaagaggta atttttccaa gcacttattg gaatccaggt attatttgtg ctgcacataa
    13861 acaaggtatt aaggtatctg atattcaata tgctgccatt actccttatc atcctgcgta
    13921 ttttaaatca ccaaaatcac attacgttgc tgataaattg ttcttatggt ctgaatattg
    13981 gaatcatgag cttttaccaa atccaacacg agagattggt tctggtgccg catattggta
    14041 tgcattagat gatgtgagat tttcagaaaa actgaattat gactatatct ttctatctca
    14101 aagtaggatt tcttcgcgct tgcttagttt tgcaattgag tttgcattaa aaaatcctca
    14161 actacagctt ttattttcta agcatccaga tgaaaatata gatttaaaga acagaattat
    14221 tcctgataat cttataatct ccacggaatc ttctatacaa ggcatcaatg aatctcgcgt
    14281 tgctgtaggt gtttattcaa ctagcttatt tgaggcatta gcatgcggca aacaaacttt
    14341 tgttgttaaa tatccgggat atgaaattat gtcaaatgaa atagattcag ggttattctt
    14401 tgcagtagaa acacctgaag aaatgcttga gaaaacaagc ccgaattggg tggctgtggc
    14461 agatattgaa aaccagtttt ttggccaaga aaaataattt ttaagttaat ctttaaaagt
    14521 gcaatatttt tcttatttta aatattgcac tttattcttt taatcatagg aactcacatg
    14581 tattctgaac agggaattaa taatactatt aacatatcta ctacatcttt aaccaatgcc
    14641 acgcaactaa ctgtgatcgg gaataataac tctgtatata taggtaataa ttgtaaaatc
    14701 gtcagctcca atattaga
//
LOCUS       DAWEWP010000039        14269 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWEWP010000039 DAWEWP010000000
VERSION     DAWEWP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.039"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(69..2045)
                     /locus_tag="WGN53_000854"
     CDS             complement(69..2045)
                     /locus_tag="WGN53_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ0139867.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPELEQAATAAVLLSMIAAPLMMGGGDALVGRFIKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WGN53_000855"
     CDS             2194..3033
                     /locus_tag="WGN53_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ0139868.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3233..4264
                     /gene="gap"
                     /locus_tag="WGN53_000856"
     CDS             3233..4264
                     /gene="gap"
                     /locus_tag="WGN53_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0139869.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4407..5132)
                     /locus_tag="WGN53_000857"
     CDS             complement(4407..5132)
                     /locus_tag="WGN53_000857"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ0139870.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVVFPDRLHIPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVVCKGL"
     gene            complement(5202..5636)
                     /gene="fur"
                     /locus_tag="WGN53_000858"
     CDS             complement(5202..5636)
                     /gene="fur"
                     /locus_tag="WGN53_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ0139871.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5855..6232
                     /locus_tag="WGN53_000859"
     CDS             5855..6232
                     /locus_tag="WGN53_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0139872.1"
                     /translation="MNKTLILALSALLGLAACSVERVSLFPSYKLKIIQGNELEPRAV
                     ASLRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKDRSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            6242..7051
                     /gene="dapB"
                     /locus_tag="WGN53_000860"
     CDS             6242..7051
                     /gene="dapB"
                     /locus_tag="WGN53_000860"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ0139873.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDTVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVALVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7048..7293)
                     /locus_tag="WGN53_000861"
     CDS             complement(7048..7293)
                     /locus_tag="WGN53_000861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139874.1"
                     /translation="MLSRQLYRISFSVETPPLGHLSFGAVESDFIWEGRNPFRIRATH
                     RATLYVSSCVLKHMPIRSLSAKCRLNNIFSDGILYGG"
     gene            complement(7311..7721)
                     /locus_tag="WGN53_000862"
     CDS             complement(7311..7721)
                     /locus_tag="WGN53_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139875.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGSRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            7944..8333
                     /locus_tag="WGN53_000863"
     CDS             7944..8333
                     /locus_tag="WGN53_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0139876.1"
                     /translation="MNVKIKYNLLEYCEYLGGIFGVYKNYLNMDIKDPNLKIRFFDFL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRRIWPDMEEMLLYFPDNPWFYVEHF
                     WWGATCPIELTQLPKIEIYEEQMKQGK"
     gene            complement(8377..8535)
                     /locus_tag="WGN53_000864"
     CDS             complement(8377..8535)
                     /locus_tag="WGN53_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139877.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGQLTD"
     gene            complement(8543..9697)
                     /gene="lpxB"
                     /locus_tag="WGN53_000865"
     CDS             complement(8543..9697)
                     /gene="lpxB"
                     /locus_tag="WGN53_000865"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ0139878.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARFVGIGGE
                     LMKAEGFESLYDQERLAVRGFAEVVRRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAERLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQSEAK
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9763..10755)
                     /locus_tag="WGN53_000866"
     CDS             complement(9763..10755)
                     /locus_tag="WGN53_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0139879.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPLDLAQFAVMLENGTKM"
     gene            11248..14007
                     /locus_tag="WGN53_000867"
     CDS             11248..14007
                     /locus_tag="WGN53_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ0139880.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFRDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVHDQVAEFMSYVMPGNVGRLKLYQDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGSDIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVHAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRIDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGSEKAKASRPEPAVKGVRHTQPAPTVAPEKKASWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTTNRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         3634 a         4469 c         3324 g         2842 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccgtttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgat gaagcgtccg
      901 accagcgcgt cccctccgcc catcatcagc ggggcggcaa tcatcgacag cagcaccgcc
      961 gccgtcgccg cctgttccag ttcgggcgaa accatatcaa gctgtccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgtgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatta atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aagatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatcc ccgccacaaa cgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgccagcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacgggacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccaaacac cagccgcctc
     1801 attgccctca gcttgggcag cgagaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaga acaaccacta taggggcaag cgaaaattcg
     2041 ttcataggtc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgtgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaaaac
     3061 cgtttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gccgttttca ataaaatatt aattacacgc cactacaaat
     3181 ttgctataat ccgccccgaa aatctaccca accctcaaca aaggaacgaa ccatgggcat
     3241 caaagtcgcc atcaacggct acggacgcat cggccgccag gttttgcgcg ccatctacga
     3301 ttatcagatt caagaccaac tccaaatcgt cgccgtcaac gccagcggca gccttgaaac
     3361 caacgcccac ctcaccaaat tcgacaccgt gcacggacgc tttgaagccg acgtatccca
     3421 cgacggcggc aacctcatcg tcaacggcga caaaatcccc ttcttctcga cccgcaaccc
     3481 tgccgaactg ccgtggaaag aactcggtgt cgatttggtc atggaatgca ccggcgcgtt
     3541 caccagcaaa gaaaaagcca aagttcatct ggaaagcggc gcgaaaaaag tcctcatttc
     3601 cgcaccgggc ggcgacgatg tcgatgcaac cgtcgtgtac ggcgtgaacg acagcgtcct
     3661 gaccgccgac atgaccgtcg tttccaacgc ttcctgcacc accaactgcc tctcgccggt
     3721 tgccaaagtg ttgagcgaaa gcgtcggcat cgtcaaaggc gcgatgacca ccatccacgc
     3781 gctgaccaac gaccaaaccg ttaccgacgt gcgccacaaa gacctgcgcc gcgcccgcag
     3841 cggcgtggaa aacatgattc cgaccaaaac cggcgcggca aaagccgtcg gtttggtact
     3901 gcccgaattg aaaggcaggc tcgacgggct tgccatccgc gtgccgaccg tcaacgtatc
     3961 attggtagat ttgagcttcc aagccgcgcg cgacaccaca gtcgaagaaa tcaacgcact
     4021 gatgaaagcc gcctcggaag caggcgcgct caaaggcgtt ttaggctaca acaccctgcc
     4081 cttggtttcc atggacttca accacaccac cgaagccagc cacttcgacg caacactgac
     4141 caaagtcgtc gacggcaata tggtcaaagt gttcgcgtgg tatgacaacg aatggggctt
     4201 cagctgccaa atgctgaaca ctgcacgccg tatgttcgga cttgaagtgc gcccgctcaa
     4261 ataacccgtc aaacaaaatg ccgtctgaaa cccgatatcg atgttttcaa gtttcagacg
     4321 gcatttttca ccgtgatttt acccggctgt cgtcatttcc aattttaatc cgccatattt
     4381 gaacctgacc gaagcataag ccgccatcaa agccccttgc acaccacttc gcgccgcccg
     4441 atttcctctt tcaacggcac ggcgttcaac atccgcagac gttcggcaaa atccgcaaac
     4501 ggcagcagct ccgaaccgaa acggcgcata tgttccgtat cctgctggca gtctatcagt
     4561 tccacgccca aatccgccaa aaacggcacg gcgcaggcaa acgcgatttt cgacgcatcc
     4621 ggttgtaatg cgaacatcga ttcgccataa aacaccctgc cgatctgaac gccgtaaaag
     4681 ccgcccgcca acctcgtttc accgctttca tcgggataat ggcactcgaa agaatgcgcg
     4741 taccccattt cgtgcagctt caaatatgcc gtctgaaact cgggcgcaat ccaagttccg
     4801 tcctgattcg ggcgcgccgc tgccgcacaa tgcgcgacca cttccgcaaa acagccgttg
     4861 accgcaaccc gatagctgcc gttgcgcagc gttttcgcca gcgagcgcgg aatatgcagc
     4921 ctgtcgggaa acaccaccgc acggggcccg accgcatacc aaaaaaacca cccgtcccgg
     4981 gaaaaccacg gaaacacgcc gttccgatac gcctcaagca gccgccccgc atccaaatcg
     5041 ccgctcacgc cgaccagccc gtcgcaccgg gccaaagcat aggcaggatc gggaaaggca
     5101 taattgtcag gggcaagcag cggaatacgc atgacagttc tccaaaaaca caggcggcat
     5161 ccaaaatcat gaatgccgtc tgaacaacaa accgtccgga tttaacgttt gcccttggcc
     5221 tgacagtcgc tgcacacgcc gtacatataa agcgcgtgat cgacgatgcg gtagccgttt
     5281 tcctcagcga ttttgtcttg cagggcttcg atttcgggat tgtggaattc cgttacctcg
     5341 ccgcacttca cgcagacgat atggtcgtgg tggtcgccct tgtccaactc ataaaccgcc
     5401 ttgcccgttt caaaatggtg gcgttgcaga atgcccgcct gctcaaactg ggtcagcacg
     5461 cggtaaatcg tcgccacacc gatttccaca ccctcttcca acaaaatgcg gtacacatct
     5521 tccgcactca aatgctcttc cgcatgcgtc tcgaacaaat ccaaaatctt caaacgcggg
     5581 ccggtaacct tcagaccgct gtctttcagt tgtgcaatat tgttgaattt ttccataata
     5641 ttcaataccc ctgtaaaaca atagacgtta taatacgcaa tttcggcctg tttgcccact
     5701 atcgcaccat agcagtttgc aatagtaaaa ccgcaccggc ggccgatgcg cggacagggc
     5761 ggataccgct taatcgtatg attatcgttc gcttttataa aatattcaag cagtgttaca
     5821 ctacccatcc cgatttgcac agaaaggcat ttccgtgaac aaaaccctca tcctcgccct
     5881 ttccgccctc ctcggccttg ccgcgtgcag cgtcgaacgc gtttcactgt tcccctcgta
     5941 caaactcaaa atcatacagg gcaacgaact cgaacctcgc gccgtcgcct ccctccgccc
     6001 cggtatgacc aaagaccaag tcctgctcct gctcggcagc cccatactgc gcgacgcatt
     6061 ccataccgac cgctgggact ataccttcaa cacctcccgc aacggcatca tcaaagaccg
     6121 aagcaatctg accgtctatt ttgaaaacgg cgtgctcgtc cgcaccgaag gcaacgccct
     6181 gcaaaatgcc gccgaagccc tccgcgtaaa acaaaacgca gacaaacaat aaggaacaca
     6241 catgacaccg ctcaaaatcg ccatcgccgg cgcaaacggc cgtatggggc gcgtcctcgt
     6301 cgaagccgtc aacaaccatc ccgacaccgt cctttccggt gcgcttgaac actcaggctc
     6361 agaagccctc gggctggacg caggctacgc cgtcggactc aaaaccggca tcgccatttc
     6421 agacgacgtt gacaccgttc tcgcacaaag cgacgtactc atcgacttca cccgccccga
     6481 gcccaccctc aaacacctgc aaaaatgcgt tgaaaaacaa gtcaacatca tcatcggcac
     6541 aacaggcttc gacgatgcgg gcaaagccgc catccgcgct gccgccgaaa aaacaggcat
     6601 cgttttcgcc gccaacttca gcgtcggcgt caacctcacc ttccacatcc tcgacaccgt
     6661 cgccctcgta ctcaacgaag gctacgacat cgaaatcatt gaaggtcacc accgccacaa
     6721 agtcgacgca cctagcggca ccgcgttacg catgggcgaa gtcatcgccg gcgcgctcgg
     6781 cagagacctc aaacaatgcg ccgtttacgg ccgcgaaggc cacaccggcc cgcgcgatcc
     6841 gtccaccatc ggctttgcca ccgtccgcgc gggcgacatc gtcggcgacc acaccgccct
     6901 cttcgccacc gacggcgagc gcgtggaaat cacccacaag gcaagcagcc gcatgacctt
     6961 tgccgccggc gccgtccgcg ccgcagtttg ggtaaacggt aaaacgggtt tgtacgatat
     7021 gcaggacgta ctcgggctga acaaccgtta acccccatac aaaatgccgt ctgaaaagat
     7081 attgttcaga cggcattttg ccgacaggct ccgtatcggc atatgtttca acacacagga
     7141 cgacacataa agcgtcgccc tatgtgttgc cctgattcgg aaggggttac gcccctccca
     7201 aataaagtct gattctaccg ccccgaagga cagatgtccg agtggcgggg tttcaaccga
     7261 aaaggaaata cgataaagtt gcctgctcag cataacaagc tacggctcgt ttaattgaaa
     7321 ctctcctgat ctaaaaattc taactctatt tcccggcaaa ctatatctat actaaaaccg
     7381 gtcggaagat catacctact gcctatcact tccagcgcac ctccataatc caagatttca
     7441 tcatcctgct ttaattctcc aagttccaat acatttttga tatttttatc ttggttcttc
     7501 gtggcgtatc catagataaa tttcagatag ctgaactgct tgaatttaat ttttacccag
     7561 tctgtataat ttggtgtagg aatctcttga ggaaagtccg gataatccac attctggacg
     7621 atgatatgga attcatcgtt tttcaaagta tagtttaata gagtgccctc atatatatac
     7681 agaaattgat aatctttttt tggaatggta taagttgtca tattatttta ccttttcaaa
     7741 aacaatgagt ttaagatttt ttgagtccaa gttggttcta ctactcttat gaaatttcag
     7801 ttgtttaaac tcttccttta ttttaaggaa tttttcaact agccgagaac aaactggagc
     7861 aaaatggacg attgagttta ggaatcaacc tgattcagta catgggcaaa gattagaatt
     7921 aaaatttaga taaaggttac gttatgaatg tgaagataaa atataatctt ttagaatatt
     7981 gtgaatattt aggtggtatt tttggtgttt ataaaaatta tttaaatatg gatatcaaag
     8041 atccaaattt aaaaattaga ttttttgatt ttttggaaga acttttatca gatggtgtta
     8101 ttgaattatg cgattatcgt gaaaatcctc caaaaatatt aactggttcg ccaaaaacac
     8161 aggtagatga attaagaaga atttggccgg atatggaaga gatgttgcta tattttccag
     8221 ataatccttg gttttatgta gaacattttt ggtggggagc aacttgccct attgaactga
     8281 cacaattgcc taaaatagaa atttacgaag aacaaatgaa acaaggtaag taagcgtatt
     8341 agtataagct ataatcataa atcatatata acaaaattaa tcagttaact gaccattata
     8401 ttttaacctt aatcccattc gggccagttc tgtttcattt ggaattcctc ttaaatactt
     8461 attgtttgat gtgccattaa tcacaatttc tccccctttt tttgtaatac gtgctgcttc
     8521 gggaaaataa tccattaacc gctcaacatc ccgcctcttc caaaaccgcg cgcgcggcca
     8581 aatccgccgt atcttttttc aacagcaggt gcaacgccct gaaatcctgt tgcagcgcgg
     8641 caaccttatc ggggtgttcg taccagtccg ccaacgccgc cgccagtttt tccggttttg
     8701 cttcagattg caataattcc ggcacagcct ccttacccaa caggatattc ggcaggccga
     8761 catgcggcac tttgattttg cgtttcacat aagcataggt cagcggcgaa atcttgtagc
     8821 tgatgaccat cggacgctta cacaacgcca cctccaaagt tgccgtaccg ctcgttacca
     8881 gcaccgcatc cgccgccctg cacactgttt cagactgtct gtcgattacc gtcagcggca
     8941 atccggcaaa ctccggccgc tgcaaaactt ccgccaaacg ccgcttcgtc gcctccgttg
     9001 cggcaggcag caggaagcgt gcggcgggat agcgttccaa caacaataat gccgtctgaa
     9061 aaaacaccgg cgccatatag tcgatttcgc tgacgcggct gccgggcagc agggcgaata
     9121 cggggatgcc ggcatccacg cccaaagttt tccgcgccgt ttcacggtcg tcttccaagg
     9181 gcataagctg cgccatcgga tgaccgacaa actccgcacg tccgcccgca tcaagataaa
     9241 gctgcggctc catcgggaac aggcacagca cgcggttgac ctgatgcacg attttgccca
     9301 cgcgttcccg ccgccacgcc caaaccgacg gactgacata atgcacggtc ggaatccccg
     9361 accgtttgag ccgctccgcc acacccagat taaaatcggg cgcatcgatg ccgacaaaga
     9421 catcaggttt caacgacagc aaatcccgta ccagccccct gcgtatccgt aaaatttccg
     9481 gcagccgcct gaccacttcg gcaaagccgc gcaccgccag tcgctcctga tcataaaggc
     9541 tctcgaaacc ttccgccttc atcagttcgc cgccgatacc gacaaaacgc gcctgcgggc
     9601 aacgcttgcg gatggcgcgt atcaggtgcg cccccaacag gtcgcccgac gcttcgccga
     9661 cactgacagc aatcaaaggg ctttttttat caaccatatt cgttcttctt cacatacttt
     9721 cacgtcccga tgccgtctga aggcttcaga cggcatcggg gttcacattt tcgtcccgtt
     9781 ttccaacatc accgcaaact gtgccaaatc cagcggcagc tccgccttca acaccagcgg
     9841 ctcgcccgtg agcggatggt tcaagtgcag ctcggacgcg tgcaaaaaca tccgcttcaa
     9901 acccaacttc tgcaaacgac ggttcgcctg ataatcgccg tagcgttcgt cgcccgcaat
     9961 cggacagcct tgagattgca ggtggacgcg gatttggtgc gtgcgccccg ttttcaacgt
    10021 cgcccgcacc aaagtcaggt gcgacagccc gacaccgtgc aaaatgccgt ctgaaaaacg
    10081 gcttaacaca cggaacaccg tatgcgccga ctgcccgtcc gcactgacgc gcaccatctt
    10141 ttcgccttgt gcgccggtat atttgaacag gggcagtttg acatggaaat tgtcgtccgg
    10201 cagtttgccc acccccagcg caaggtagat ttttttgggg tggtcgttac ggatggcttc
    10261 gtgaagtttg acgagcgcgc tgcgtttctt cgccaccatc aacaagccgc tcgtatcctt
    10321 gtccaaacga tgaaccaact ccaaatactt cgcctccgga cgggcgcggc gcaactgttc
    10381 gataacgccg aaactcacgc cgctgccgcc gtggacggca acgccggacg gtttgtcgat
    10441 gaccaaaagc gcatcgtctt cgtaaacaac gtcaaacgca cgcgccggta cggcggcacg
    10501 cctttcagac ggcatttcct tctccgccac gcgcacaggc ggaatccgca ccgtatcccc
    10561 ctccgcaata cggctgtcgg gtttgcagcg tttcttgttc aaccgcacct cgccggcgcg
    10621 gataatgcgg tggatatggc tcttgggcac gcccttgagg atttttatca gatagttatc
    10681 aaggcgttgc cccgcctcat gttcggcgac ccctatcaag ctgaccgaat ctttgcttat
    10741 ttcgtgcgtt ttcatctata atccgaacat ccgtttcagc aaaaagcgcg cccgccgcgc
    10801 ctcctgaaac ggtttacttt aaaacggaat tttatattaa aaacgcactc cgcaaagcat
    10861 tttccctgcg cctgttccca gccggcaggc gcagaacgta gtcaagtttg aattgatttt
    10921 gccgtttcgg cgcggaagta agacggcagc cgggcccaag ccccaaacgc aaaccgcccg
    10981 aaaacacagg catcacgata acaagaagaa gccggcaccc atttttccga ccataccgct
    11041 cggaacgcgg cagacagacc gccgcatcga tctttatagc cttctgtatc cgacccttcc
    11101 gcacacggtt ctccggaacg tgcatccctg cggattttca actcaaaaac tgattaccgg
    11161 ttttatccga agacatcgga aaacccagcc ttacggaatg ccgaaccgga ccggcgcgcc
    11221 cgaatgccgc ccgtccacga ggtgatcatg aaaagaatgt tattcaacgc aacgcaggcc
    11281 gaagagctgc gcgttgccat cgtcgatggc caaaaccttt tggacttgga catcgaaacg
    11341 ctgggcaaag aacagcgtaa aggcaatatc tacaaaggta tcattacccg catcgagccg
    11401 tcgctggaag catgtttcgt cgattacgga accgaccgcc acggcttttt gccgtttaag
    11461 gaagtatcgc gttcatattt ccgcgactac gaaggcggca gggcgcgtat tcaggacgtg
    11521 ctcaaagagg gcatggaagt cattgtccaa gtcgaaaaag acgaacgcgg caacaaaggc
    11581 gcggcgctga ccactttcat cagcctggcc ggccgctatt tggtattgat gccgaacaac
    11641 ccgcgcggcg gcggcgtatc ccgccgtatc gaaggcgaag agcgtcaaga gttgaaagcc
    11701 gccatggccg aactcgacat tccaaacggc atgagcatca ttgcccgtac tgccggcatc
    11761 ggccgcagtg cggaagagtt ggaatgggac ttgaactacc tcaaacaact ctggcaagcc
    11821 attgaagaag caggcaaagc acaccatgac ccttacctgc tctttatgga aagctcactg
    11881 ctgatccgtg cgattcgaga ctatttccgt cccgatatcg gtgagatttt ggtggacaat
    11941 caagaagttc acgaccaagt tgccgagttc atgagctacg tcatgccggg caatgtaggc
    12001 cgtctgaaac tctatcaaga ccacacgccg ctgttctccc gtttccaaat cgaacaccaa
    12061 attgaaagcg cgttctcacg cagcgtcagt ctgccttccg gcggcgcgat tgtgatcgac
    12121 cacaccgaag ccctggtttc catcgacgtc aactctgccc gcgccacacg cggctctgac
    12181 attgaagaca ccgcgttcaa aaccaatatg gaagccgccg aagaggttgc ccgtcaaatg
    12241 cgcctgcgcg acttgggtgg tttggtcgtc atcgacttca tcgacatgga aaatccgaaa
    12301 catcagcgcg atgttgaaaa cgtcctgcgc gatgcgctca aaaaagaccg cgcccgcgtg
    12361 caaatgggca aactttcgcg tttcggcctt ttggaattga gccgccaacg tctgaaaccg
    12421 gctttgggcg aaagcagcca cgtcgcctgt ccgcgctgcg ccggcacagg cgtcatccgc
    12481 ggcatcgaat ccaccgccct gcacgttttg cgtatggttc aggaagaagc gatgaaggac
    12541 aacaccggcg aagtgcatgc acaagtgccc gtcgatgtcg ccaccttcct gctgaacgaa
    12601 aaacgcgccg agctgtttgc gatggaagag cgtttggatg tcaacgtcgt cctgattcca
    12661 aacatccact tggaaaatcc gcactacgaa atcaaccgca tccgcattga tgacgtagaa
    12721 gaagacggcg aaccgagcta caaacgcgtt gccgagccgg aagaagacga atccgccaaa
    12781 cctttcggca gcgaaaaagc caaagcttcc cgtcctgagc cagctgtcaa aggcgtacgc
    12841 catacccagc ctgcaccgac tgtcgcccct gagaaaaaag cctcttggtg ggacagcttc
    12901 aaagcttggt tgaaacgcat tttcggcggc agcgaaaccc aagccgcgcc cgctgccgaa
    12961 acctccgaaa aacgcagcac gacaaaccgc agcggcagcc gcgccaacaa ccgccgccaa
    13021 aacccgcgcc gcagcaaacg cgaaggcagc aaagtagaag tccgcgaagt ggcaggcaaa
    13081 actgccggac aggaagcgcg cgccgacaaa gccgaaacgc gcaacaacgg caaccgccgc
    13141 cgcaacgaac gcggcgaccg tgccgccgga cgcgccgacg aagcggaaat ccaagaccgc
    13201 aacatacagc ctgccgcaac cgttgcagat gccgcaccgt ccgaaaccga agtgcaaacc
    13261 ggaaaacgcc gccgcaacgg cagccgcagc gaacgcggcc aaaccgcgcc ggaaaccgcc
    13321 accgttgccg aaacaaccgt tcagacagcg gaaaacacgc cgtccgaacc gcataccgca
    13381 gaagacaaag gcagcaagcc caaatccgaa cgcaaccgcc gcgaacgcga cagccgagat
    13441 gccaaagaac gccgcgagcg caacaatcag cgcgaccgcc gtcaaaacgg caaaaaacgc
    13501 aatattccgt ctgccgccaa aatcgagcag tacctgaata ttcacgacac cgccgacaaa
    13561 gtccgttccg ccgccgcgca cgttttcggc gaaaccgacg caaacgcgcc gattaccgtc
    13621 agcattgccg atccggttgc agaaagggat cttccgacag catctcccgc cgtttcaaac
    13681 ggcgacgcac cggtttatga tgcggcggaa aaaatccgcc gtgccaccgc cgccatcctg
    13741 cccgaaggcg cgacaccgaa agccgaagca caggaaatgc cgtctgaaac cgcaaccttt
    13801 acggctgcgg cggaacaggc acgggaaacc gcacaaaccg gcggactcgt cctgatcgaa
    13861 accgaccctg ccgcattgaa ggcatgggcg gcacaacccg aagtccaagc cggacgcggt
    13921 ttgcgccgtt ccgaacagcc caagccgtct gaagtcgcaa ccgtccctgc cgaagaaatg
    13981 atccaagtcg aaacccggca aggctgaacc gacggcggca aaaagaggtt ctgttccgca
    14041 gaacctcttt tttacatggg ttcggatacc tgcaatgccg tctgaaactt cgccattccc
    14101 gtgattaccg aaacattcct ccattcccat gattcccgca acattccgtc attcccatga
    14161 ttcccgcaac attccgtcat tcccgtgaaa acgggaatct agaacctcta aactttcaga
    14221 taatttttga atattgccgc cgcccaaagg tctagattcc cacaacctt
//
LOCUS       DAWEWP010000040        14069 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWEWP010000040 DAWEWP010000000
VERSION     DAWEWP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14069
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.040"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(407..1672)
                     /gene="ftsY"
                     /locus_tag="WGN53_000868"
     CDS             complement(407..1672)
                     /gene="ftsY"
                     /locus_tag="WGN53_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ0139881.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEEVE
                     SLTESVKEQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            1817..3385
                     /gene="msrAB"
                     /locus_tag="WGN53_000869"
     CDS             1817..3385
                     /gene="msrAB"
                     /locus_tag="WGN53_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ0139882.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            complement(3487..3972)
                     /locus_tag="WGN53_000870"
     CDS             complement(3487..3972)
                     /locus_tag="WGN53_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ0139883.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(3999..4847)
                     /locus_tag="WGN53_000871"
     CDS             complement(3999..4847)
                     /locus_tag="WGN53_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0139884.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            complement(4941..5942)
                     /locus_tag="WGN53_000872"
     CDS             complement(4941..5942)
                     /locus_tag="WGN53_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0139885.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            6001..6978
                     /locus_tag="WGN53_000873"
     CDS             6001..6978
                     /locus_tag="WGN53_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ0139886.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVNALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHALPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTPYPPKIRKNVMRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTP
                     VYYQDGSYSKNMNYNQYRPERHAVLPDQTDGSEQDVRGQNWQKPKFQNR"
     gene            7033..8403
                     /gene="glmU"
                     /locus_tag="WGN53_000874"
     CDS             7033..8403
                     /gene="glmU"
                     /locus_tag="WGN53_000874"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ0139887.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVIGHGKDQVLNAVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDVPLIDVKTLKTLLEAAGNEVGLLTDVPADPTGLGRIIRDGNGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVHT
                     SYLAAGVNNKLQLAELERIFQTEQAQELLKAGVTLSDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVEIGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNRIGPYARLRPQ
                     AKLAADVHIGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGNEVRIGSNCVLVAPVTLGNKVTTGAGSAITRNVEDGKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            8480..8809
                     /locus_tag="WGN53_000875"
     CDS             8480..8809
                     /locus_tag="WGN53_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ0139888.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAALQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(8881..10146)
                     /gene="efeB"
                     /locus_tag="WGN53_000876"
     CDS             complement(8881..10146)
                     /gene="efeB"
                     /locus_tag="WGN53_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ0139889.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQLDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(10410..11576)
                     /locus_tag="WGN53_000877"
     CDS             complement(10410..11576)
                     /locus_tag="WGN53_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ0139890.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     VSELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            complement(11573..12412)
                     /locus_tag="WGN53_000878"
     CDS             complement(11573..12412)
                     /locus_tag="WGN53_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ0139891.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMVLWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            12623..13948
                     /locus_tag="WGN53_000879"
     CDS             12623..13948
                     /locus_tag="WGN53_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ0139892.1"
                     /translation="MKKYLFRAALYGISAAILAACQSKSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
BASE COUNT         3244 a         4211 c         3578 g         3036 t
ORIGIN      
        1 aattcctaaa actcaaaatt tcatcattcc tacaaaaaca gaaaaccaaa atcagaaacc
       61 taaaatcccg tcattcccac gaaagtggga atccagaatc tcggactttc agataatctt
      121 tgaatattgc tgttgttcta aggtctggat tcccgcctgc gcgggaatga cggcggagcg
      181 gtttctgttt tttccgataa atgccgcaat ctcaaatccc gtcattccca cgaacctaca
      241 tcccgtcatt cccacgaacc tacatcccgt cattcccacg aaagtgggaa tccagaatct
      301 ctaaagcttc aactaacctt tgaatattgc cgttatccca aggtctagat tcccgcctgc
      361 gcgggaatga cggcatcggt ctgctgtttt cggacggcat ttcggctcaa tccagcagtg
      421 cgtccacaaa cgcgcgcgcg tcaaacgggc gcaaatcgtc tatgccttcg cccacgccga
      481 tgtagcggac ggggacgggg cggtcggaag caagcgcggc gaggatgccg ccttttgccg
      541 tgccgtcgag tttggtcacg attaaaccgg tcagccctaa tgcgtcgtca aaggctttga
      601 cttggttgac ggcgttttgc ccgatgttgg catcaagcac gacgatgatt tcgtgcggcg
      661 cgtcgggcat ggctttttgc agcacgcgtt tcactttttt gatttcttcc atcaaatgaa
      721 gctgcgtggg caggcggccg gcggtgtcgg caagcacgat gtcgatgccg cgcgctttgg
      781 cggcttggac ggcatcgaag cacacggcgg cggaatcgcc cgtggtttgc gaaatcacgg
      841 taacgttgtt gcgttcgccc catgcctgaa gctgctcacg ggcggcggcg cggaaagtgt
      901 cgcctgccgc cagcaatacg gatttgccct gcgcttggaa atatttggcg agtttgccga
      961 tagacgtggt tttgcccgcg ccgttgatgc cggcaagcat gatgacaaac ggctctttgg
     1021 tttcgggcaa gaccaatggt ttttccagag gcttaatcag gtcgtacaag gcttctttca
     1081 acgcgccgcg caattcgttg ccgtctttca gccctttgag gctgacgcgg tcgcgcacgt
     1141 ctttcatcag gtattcggtg gcttccatgc ccatatcgct ggtaatcagc acggtttcca
     1201 gctcttcgta caaatcctcg tcgatttgtc cgccgccgaa cacgcccgcc agcgatttcg
     1261 ccattttgtc gcgcgatttg gtcaaacctt gtttcaaacg cgccgcccaa ccgagcttgt
     1321 gttcttcagt tgttgcaacg gcttcttgaa cttgcccgac agcctcgccg acggtttcgg
     1381 caatggcttc ttttgccgct tcgacttgtt ccgctgcttt ttccgccgct tcctctgctt
     1441 cagacagcat ctcggcaacg gtttccttta cttgttcaac cgcaccgctg acggtttcaa
     1501 cagccgattc gacctgctct ttgacgcttt ccgttaaaga ttcgacttct tctttaatat
     1561 tttcaattac ttgatcggtt tcggactctg cttttgctgc ggtttcctga atttgagcct
     1621 cctcgagagc cggcgtttcc tgttttttct tgcgacggaa gaagctgaac attgaatttt
     1681 ccttttaatt ttagaaactt gaaacagggc gtattgtagc gtattttacg cggcaaggtt
     1741 gtctgaaaat ccgagctgta aggtttcggc atctcaaacg tctaatcatg caaaccgtcc
     1801 acacaggaaa ctcaaaatga aacaccgtac tttcttttcc ctttgcgcca agttcggctg
     1861 cctgcttgcg ctgggtgctt gttcgcccaa aatcgtcgat gccggagccg cgaccgtgcc
     1921 gcacacttta tccactttga aaactgcgga caaccgcccc gccagtgttt acttgaaaaa
     1981 agacaaaccg acgctgatta aattttgggc gagctggtgt cctttgtgtt tgtccgaatt
     2041 gggacagacc gaaaaatggg cgcaagatgc aaaattcagc tccgccaacc tgattaccgt
     2101 cgcgtctccg ggctttttgc acgagaaaaa agacggcgac ttccaaaaat ggtatgccgg
     2161 tttgaattat cccaagctgc ccgtcgtaac cgacaacggc ggcacgatcg cccaaagcct
     2221 gaatatcagc gtttacccct cgtgggcgtt aatcggtaaa gacggcgacg tgcagcgcat
     2281 cgtcaaaggc agcatcaacg aagcgcaggc gttggcgtta atccgcgacc cgaatgccga
     2341 tttgggcagc ttgaaacatt cgttctacaa acccgacact cagaaaaagg attcaaaaat
     2401 catgaacaca cgcaccattt acctcgccgg cggctgcttc tggggcttgg aagcctattt
     2461 ccaacgcatc gacggcgtgg ttgacgcggt atccggctac gccaacggca acacgaaaaa
     2521 tccgagctat gaagacgtgt cctaccgcca tacgggccac gccgaaaccg ttaaagtgac
     2581 ctacgatgcc gacaaactca gcctagacga catcctgcaa tatttcttcc gcgtcgttga
     2641 tccgaccagc ctcaacaaac agggcaacga caccggtacg caataccgca gcggcgtgta
     2701 ctacaccgac cccgccgaaa aagccgtcat cgccgccgcc ctcaaacgcg agcagcaaaa
     2761 ataccaactg cccctcgttg ttgaaaacga gccgctgaaa aacttctacg atgccgagga
     2821 ataccatcag gactacctga ttaaaaaccc caacggctac tgccacatcg atatccgcaa
     2881 agccgacgaa ccgctgccgg gcaaaaccaa gaccgccccg caaggcaaag gcttcgacgc
     2941 ggcaacgtat aaaaaaccga gtgacgccga actcaaacgc accctgaccg aagagcaata
     3001 ccaagttacc caaaacagcg cgaccgaata tgccttcagc cacgaatacg accatttgtt
     3061 caaacccggc atttatgtgg acgttgtcag cggcgaacct ttgttcagct ccgccgacaa
     3121 atatgattcc ggctgcggct ggccgagctt cacgcgcccg attgatgcaa aatccgttac
     3181 cgaacacgat gatttcagct acaacatgcg ccgcaccgaa gtgcgcagcc acgccgccga
     3241 ttcgcatttg ggacacgtct tccccgacgg cccgcgcgac aaaggcggac tgcgctactg
     3301 catcaacggc gcgagcttga aattcatccc gctggaacaa atggacgcgg caggctacgg
     3361 cgcgttgaag agtaaagtga aataagccgc accgccgcct aacccgacaa aataccgtct
     3421 gaaacctgca acgtttcaga cggtattttt tatccggcgg ggattctgtc cagacggaga
     3481 ttttgtttag acagcatcgc cgccgttttc aatcagcccc gccaaccgtt ccaacgcgaa
     3541 ggcgaccgcc tgcgcgcgga cggattcgcg gttgccgtca aaacggcgca ttgcttcgca
     3601 acttccgccc ggaaaggcaa acccgaacca aaccgtgccg acgggtttgc tttcgctgcc
     3661 gccgcccggt ccggcgatgc cggaaatacc gacggcgtaa tccgcctgcg ccacggcttt
     3721 cgcgccgcgc gccatctcat agacggtttg gcggctgacc gcgccgtgtt cgagcagggt
     3781 ttcgggcaac acgcccaagc ggtcttcttt ggctttgttg ctgtatgtta caaaactctg
     3841 gtcgaaccat tgcgaactgc ctgcaacgct tgtgaatgcg gcggcaagca ttccgcccgt
     3901 acaggactcg gcacagctta cggtttgacg ttttttcgtc aggtttcggg cgatggtgtg
     3961 cagcgcgtcc attttcccac tcccctttca gacggcgttt aagaattgat gatgtgtatg
     4021 tcgcgttgcg ggaacgggat gttgatattg actttgcgga ggttttcgac cacttgttcg
     4081 ttcaaatcgc attgcagcgt ccagcggtct gcttcgtttg cccaagccca taatgtgatt
     4141 tcgatggcat tgtcgcccaa ggcggtgatg taggcggcgg cctgccgctc ttcgttttga
     4201 acgctcaagg ggtgttcgac ggcggctttc aacaccgcct ctttcgccac tttcaaatcg
     4261 cagttgtaat cgacgccgac tatcacttgg gcgcggcaaa gcggcagggt ggaacggttg
     4321 acgatgctgt tgcccatcac cacgctgttg ggcagcacga cttcttcgtt gtcggtcgtc
     4381 cgcaaagaag tctgcaccat tttaatctct cggacatatc cttcaaaacc gccgacgcgg
     4441 ataaaatcgc cgactttgaa cgggcgaaac aggataatca gcgcaccggc ggcaaaattg
     4501 gacagctggt ctttcaagga caacgccacc gccaaacccg cgccgccgat taaggcggtt
     4561 acggatgttg tggaaacgcc caatctgccc aatgcggcaa taatcaccaa aatcaataag
     4621 ccgatattgg caacattaca caaaaaacta atcagcgtgg catcgacctg cgcgcgcgtc
     4681 atcgccgccc tcatcacggc gacaatgcgt ttcgccgccc attttccgac caaaaaaata
     4741 agcagcgcgg cggcaaggtt cagcccgaac gcccacgcct tttcagccag atgctcccaa
     4801 ccggaaacac tgatcaggtg taaaaaatca aattgtttga agtccattgt tttcctcttg
     4861 atcgaacatc cgccccgtgc ggcgtaatcg gcacaggtgt aaaaatgccg tatgaagccc
     4921 tgcggggcgg tatgtttgtt ttatttcaaa accgttctaa tccaacggga aacccatccg
     4981 cgctggttcg catcaatatc gcgctgcgcg ggggcggtgg tgtgcgtcgg ggcttgggcg
     5041 aagcggaaca cgtcgggcag gcgcgtgttt ttgacggcgg ggtaaaccca catttcggac
     5101 ggaaccgcct gctgcacttc ccgactttgc agccattgca ccagttttgc cgccagcttc
     5161 ggctgtttcg cgcccttcaa gactgccgcg ccttcgacct ggcggaatac gccgcctttt
     5221 aaaaacaggt tgcccgtcgg cggctcgctg tatttgcctt tggaaaaata cacttccgcc
     5281 gccgggctgg cggcataacc gaccaccagc ggatacgcgc cgccgttgtg cgaaaagtcg
     5341 gtgtaatacg cctcgctcca gcctttggcg accttcacgc cgttctgccg catctgtgcc
     5401 caccatttga acgcgccttc ttcgcccaga ccgctgatgt tcgccatcag gaagcccagc
     5461 cccggggacg acgtggcggg agacggcacg accaataggt ttttatattc ggggcgggtc
     5521 aaatcctgca gggtttgcgg caggggcagc tttttgcctt caaaccattt tttgtcgtaa
     5581 ttgatggaca catagccgta atcgaccgcc aaagccgaag gcagcccgac cgcgacgggg
     5641 gcggattcgg gttgcgctgc cgccaaaatg cccatttccc gcgccttgcc gatattggcg
     5701 ttgtccaaac cgtacaccgc gtcggcaatc gggttggcgc ggctcaaaat cagtttgttg
     5761 agcatttcgt tcgcgccgcc cgcctgaata atcgacacct tcgcatcgtt tgcccgctcg
     5821 aagcgcgcaa tcagcccttt gggcaggctg aacgacttat gcaccgccag cctgacttcc
     5881 gtctgcgcct gcaggtatgc cgaaaccgcc agcagcggca gcagccaaat tttccgtttc
     5941 attccgagtc ctcctctatt cgctgtaaaa taacattcta acaaattttc acggttcaac
     6001 atgcaagaaa acccgaccgt gtggctgttc gacctcgaca acacgctgca caatgccgat
     6061 gccggcatct tcacactcat caaccgcgcc atgacacgct atatggcacg ccgcctcaaa
     6121 ctctccgaat ctgccgcgtc cgacctgcgt caagactatt ggcaccgcta cggcgcaacg
     6181 ctcgccggac tgcaaatcca ccatcccgaa atcgacatcg ccgaattttt gcgcgaaagc
     6241 catccgatcg atgcaatcct gaccaggctg cacggcatgc ctgaaacaca aaacaccctg
     6301 agccgcctaa aagggcgcaa ggcggttttt tccaacggcc cgtcgtttta cgtccgtgcc
     6361 gttgtcaacg cactcggttt ggaaaaccgt ttcgacgcgc ttttcggcac ggatgatttc
     6421 gggctgctgt acaaacccaa tccgcaagcg tatctcaatg tctgccgcct gttggacgta
     6481 ccgcccgaat gctgcattat ggtggacgac agcgcggaca acctgcatca ggcaaaggcg
     6541 ctgggtatga aaaccgtccg gttcggtgca aaatcccacg cgctgccctt tatcgatgcc
     6601 tccgtaagcg atatggcgca actggctcgg tatgcagaaa ctttgtcaga acaccgccaa
     6661 aatcattaca atacccccta ccccccgaaa atacgaaaga atgttatgcg taaaaccttc
     6721 cttatcctga tgactgccgc cgcccttttg tcgggctgcg cgtgggaaac ttatcaagac
     6781 ggcaacggca agaccgccgt ccgtcaaaaa tatcccgccg gcacgcccgt ttattaccaa
     6841 gacggcagct actcgaaaaa tatgaactac aaccaatacc gccccgaacg ccatgccgtg
     6901 ctgcccgacc aaaccgacgg ttccgaacaa gacgtacgcg gacagaactg gcaaaagccc
     6961 aaatttcaaa accgataaac ctaccctatg ccgtctgaag ctgcttcaga cggcattgca
     7021 caggaaaccg tcatgccgca aaacacttta aacatcgtta tcctcgccgc cggcaaaggc
     7081 acgcgcatgt attccaaaat gcccaaagtg ctgcaccgca tcggcggcaa gcccatgctc
     7141 gggcgcgtta tcgacaccgc cgccgcgctg aatccgcaaa acatctgcgt cgtcatcggg
     7201 cacggcaaag accaagtttt aaacgctgtc aaacgcgatg tcgtttgggt tgaacaaacc
     7261 gaacagctcg gtaccggcca cgccgtcaaa accgccctgc cccacctttc cgccgaaggc
     7321 cgcacgctgg tactgtacgg cgacgttccc ctgattgacg ttaaaaccct caaaaccctg
     7381 ctcgaagccg ccggcaacga agtcggtctg ttgaccgacg ttcccgccga cccgacaggc
     7441 ttgggccgca tcatccgcga cggcaacggc agcgtaaccg ccatcgtcga agaaaaagac
     7501 gccgacgccg cccaaaaagc cgtaaaagaa atcaataccg gcattttggt tctgcccaac
     7561 gccaaactgg aaaactggct gaacagcctc tccagcaaca atgcccaagg cgaatactat
     7621 ctgaccgacc tcatcgccaa agccgttgcc gacggtatta aagttcatcc cgtccaagtg
     7681 cacacctcct acctcgccgc cggcgtgaac aacaaactcc agctcgccga actcgagcgt
     7741 attttccaaa ccgaacaggc tcaagaattg ctcaaagcag gcgttaccct gagcgacccg
     7801 gcacgtttcg atttacgagg ccgtctgaaa cacggacaag atgtcgtaat tgatgtgaac
     7861 tgtatctttg aaggcgaagt cgaaatcggc gacaacgtcg aaatcggcgc aaactgcgtc
     7921 atcaaaaacg ccaaaatcgg cgcaaacagc aaaatcgccc ccttctccca cctcgaagac
     7981 tgcgaagtcg gcgaaaacaa ccgaatcggc ccgtacgccc gtttgcgtcc gcaagccaaa
     8041 ctagccgccg acgtacacat cggcaacttc gtcgaaatca aaaacgccgc catcggcaaa
     8101 ggcaccaaag ccaaccacct cacctacatt ggtgatgccg aagtcggcag caaaaccaac
     8161 ttcggcgcag gtacgattat tgccaactac gacggcgtgc acaaacacaa aaccgtcatc
     8221 ggcaacgaag tgcgcatcgg ttcaaactgc gtactggttg cccccgttac cctcggcaac
     8281 aaagtaacca caggcgcagg cagcgcgatt acccgcaacg tcgaagacgg caaactcgcc
     8341 ctcgcccgcg cccgccaaac cgtcatcgaa ggctgggtga ggccggaaaa agacaaacaa
     8401 taaagccatg ccgtctgaag ccggtttcag gtttcagacg gcatcaaacc cctccgatat
     8461 cagataagga ctgcaaacca tgtcattacc cccatgccca caatgcgcct ccgaatacac
     8521 ctatgaagac ggcggacaat acatctgccc cgaatgcgcc cacgaatgga atgaaaccga
     8581 atccgccgcc gaccttgcgg ctcaagtgct cgatgccaac ggcgcggcac tgcaaaacgg
     8641 cgataccgtc atcctcatca aagacctcaa ggtaaaaggc agctcgatgg tgatcaaaca
     8701 aggtacaaaa gtcaaaggca tacgcctgca ggaaggcgat cacaacatcg gctgcaaaat
     8761 cgacggcagc gcgatgaatt taaaatccga attcgtcaaa aaagcctgac cgcccaaaaa
     8821 caagaaacgc cgtccgaacc cgtttggcaa ggttcggacg gcgtttttta tatggcggat
     8881 ttatacgccc agcagccctt gccccaaaaa gccgcctttt tccacgccgg gcaagacgaa
     8941 gaaatagccg ccgccgaagg ggctgatgta ttcttccagc ggttcgccgt tgaggaggtt
     9001 ttgcacgaag atgaatccgt cggcaaggtt tgcctgatag cagacgaaca ccagtccgac
     9061 atcaagctgt ccgcttgagg caagtccgcg cgaatagctg taggcgcggc ggaagaggcg
     9121 gtgttttttg aggaattcgg gatcgcgcgg attcgccagg cgtatatggc tgtctttggg
     9181 cgtggtattc ccctcggggt ctttggcaaa atccagttgg tcggcttctt ttttgccgtc
     9241 catcggcgca ccgctgtatt tgcgccgccc gaaaatgtcg gtttgctctt gaagcggcgt
     9301 cctgtcccaa aactcgacaa agtggcggat aaggcggact gcctgatagc tgccgttttt
     9361 cgcccactcc ggttcgtcga ggctgttggc ggccacaccc gtccacaaaa cctcgtcggc
     9421 ggttttgggg tcggaaactt tggggttgcc cgtgccgtcg cggaagccca acaggttgcg
     9481 cgccgccatc gcgccgggtt cggatttagg ctgccacccg tcgatactcc agcggataac
     9541 ggcggtttgg acggtgtgtt tgatgatgtc gcgcagggcg gcttggcagg tttcgggggt
     9601 gaaggcacag atttgcaggc tcaaatcgcc gtcgcaccag cttttttgca gcttatcgtt
     9661 ggagaagtcg cgcatttcct gcaaatgaat cggttttttg tctttgagtc cgaaccggcc
     9721 gtcaaacagg ctgctgccca cccccacggt aacggtcaac ccgtcggggt tgaaggcttt
     9781 gcccaaaatg ccgctgccgg ctggcggaag tttgtcgtcg ccgtcttggt attcgccgcc
     9841 ttgggtgaga aactcgatgc gggcggtcag cgtgcggaac aggttttcca gctgcttggc
     9901 actttgtgcg gttacgtcga aggcgcacat aatcgaaaac gcctgctgcg gcgtaacgat
     9961 gcctgcctga tgttcgccgt agcagggata ggcttggggc gagtgttggc tttcggcggt
    10021 gcgttcggcg gtttcgcccc gttttttgcc gccgagataa cctccgattg cgccgactgc
    10081 tccggctgcg atcgcggttt taaaaagagt gcgcctggtc ggttgtgcgg gttggttttt
    10141 gctcatggtg tttccttcaa tatccgtgca gcaggatggg tatccctgca cgccgttttt
    10201 ccattaaccg atagcggcag gttgaaatcc cgccctgcaa tatatggcgg atatagtgga
    10261 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt ataaatagta cggcaaggca
    10321 aggcgaggca atgccgtact ggtttttgtt aatccactat aaaacttcag acggtatctg
    10381 ccgccaaata ccgtctgaac gcttgcggct tatttcaagc cgagtatgcc gcgaagttgg
    10441 gcaaggtctt cggcaagcgc gttaatagag gcctgtaacg ctttgcggtc ggcttcgccc
    10501 agcttgtcgt aggtttcaaa accgtcttta gtccggtatt tcgccagaat ttcgttgacc
    10561 tgtttgaagt tggtatcggt tttttccaac aaggctttgt ttttggcctc gatcagcgga
    10621 cggaacaaat cgacgatttt tttagatccg tccacattgg cttggaagtc gctcaaatcg
    10681 gtgtggctgt accggtcttc ttcgccgctg attttactgc ccgccacttc ttcaatcagt
    10741 tcggacacgc cgccgaccac cttgcccgga ggaaacgcca atgcgtcgat ttctttttgc
    10801 agggcttcga catcggtcat cagtttcgct gcaatttcct tcacgccgga cacgtctttt
    10861 tccacccaaa gggcgtattc gatacggtgg aagccggtaa atccggcatc tttcgcgccg
    10921 tctttgaagt cgtcttcacg cgcatcgatg acggggtcga gttcgctgaa aagctcggca
    10981 atcggttcga tgcgttcgta atggacgcgg gtgtcggcaa acagggattt cgccttttca
    11041 atgtcgcctg ctttgacggc ttcggtaaag gttttggttt tcgccaccag ctctttaacc
    11101 tcgccttgaa cgtaggcttt atagtcggcg agcggttggg acagtttttc caaatccgct
    11161 tcgttggcgg tgtctttaaa gccgctgtcg gttaccacca gcttgccgcg cggattggtc
    11221 aaaagaccgc aagtcatttc gtattcgccc ggcaacaggg tgacggtcat tttatcggaa
    11281 agtccggggg cgatgttttc gcgctcgtcc accaccatca cgcctttcag gatttcccat
    11341 tcgagcttgc ggccgctgtt gtttttaata ttgaacacaa cctgtccgct cggcacggtc
    11401 agttccatcg gttcgcaggc attgtcgttg acggcgatac tgaccgaacc gccctcgttg
    11461 gcggtttgcg cctcaccgga cgcggccggc gcagctttct ccgcctccgg cggctggcac
    11521 gcggtcaaac ctaaggcaag catcacggac aatgcggtca aattgaattt tctcatttca
    11581 gctcctcttt acgggttaaa gtttcagacg gcctgctgcc gcacaaaaac caagtcatga
    11641 cgggaataag gtacagcagc caaaccaagg cctcgccctg cgtcggatgg tcggtatagc
    11701 cgaaaaatcc gccgagcagc acacctaacg gactgtcttc gtgcaaatat tttgatgagt
    11761 cgaacacaat gtcctgaagc gcgttccaaa tgcctgcttc gtgcagcgcg cgcagcgagc
    11821 cggcaagcag gccggcggca acgacaatca gaaacgcccc cgtccaacgg aaaaacttcg
    11881 ccagattcag gcgcatccca ccctgataaa tcaacgcgcc aatcacggcg gcagccaaaa
    11941 cccccgctac cgcaccggcc ggcatctgcc aagtcgggct ctgtttgaat acggcaagca
    12001 gaaaaaaaac gctctccaaa ccttcgcgcg ccacggcaag aaacgccata ccgaccaagg
    12061 cccatccttg tccgctgccc cggttcaaag ccgcctgcac agaatcctga agctgctgct
    12121 tcatcgaacg cgccgccttt ttcatccata aaaccatata agtcagcatc gcgacggcaa
    12181 ccaggccgat aatgccgacg acgaactcct gctgcttttg gggaatctcg cccgttgccg
    12241 aatggatgcc gtaccccagc cccaaacaca tcaaagaagc aaggacgacc ccgaaccaga
    12301 ccttaggcat cagtttggaa tgtccggact gtttcagaaa accggcaacg atgccgacaa
    12361 tgagcgcggc ttcgataccc tcgcgcaaca taattaaaaa agcgaccagc ataaacgcga
    12421 acgaacaagg atgatgaata atatattatc ggaatatttt cattgcttgt aaatacaaat
    12481 gcaagttatt tttatctgca gtaccgcgcg gcggaaagtt ccgcaggctg cagctgcgcc
    12541 ctgtgttaaa atcccctctc cacggctgcc gcaacgccgc ccgaaaccat ctttcttatt
    12601 actgccggca acattgtcca ttatgaaaaa atacctgttc cgcgccgccc tgtacggcat
    12661 ctccgccgcc atcctcgccg cctgccaaag caagagcatc caaacctttc cgcaacccga
    12721 cacatccgtc atcaacggcc cggaccggcc ggccggcatc cccgaccccg ccggaacgac
    12781 ggttgctggc ggcggggccg tttataccgt tgtgccgcac ctgtccctgc cccactgggc
    12841 ggcgcaggat ttcgccaaaa gcctgcaatc cttccgcctc ggctgcgcca atttgaaaaa
    12901 ccgccaaggc tggcaggatg tgtgtgccca agcctttcaa acccccgtcc attcctttca
    12961 ggcaaaacag ttttttgaac gctatttcac gccgtggcag gttgcaggca acggaagcct
    13021 tgccggtacg gttaccggct attacgaacc ggtgctgaag ggcgacgaca ggcggacggc
    13081 acaagcccgc ttcccgattt acggtattcc cgacgatttt atctccgtcc ccctgcctgc
    13141 cggtttgcgg agcggaaaag cccttgtccg catcaggcag acaggaaaaa acagcggcac
    13201 aatcgacaat accggcggca cacataccgc cgacctctcc caattcccca tcactgcgcg
    13261 cacaacggca atcaaaggca ggtttgaagg aagccgcttc ctcccctacc acacgcgcaa
    13321 ccaaatcaac ggcggcgcgc ttgacggcaa agcaccgata ctcggttacg ccgaagaccc
    13381 tgtcgaactt ttttttatgc acatccaagg ctcgggccgt ctgaaaaccc cgtccggcaa
    13441 atacatccgc atcggctatg ccgacaaaaa cgagcatccg tatgtttcca tcggaaaata
    13501 catggcggat aagggctacc tcaaactcgg acaaacctcc atgcagggca ttaagtctta
    13561 tatgcggcaa aatccgcaac gcctcgccga agttttgggt caaaacccca gctatatctt
    13621 tttccgcgag cttaccggaa gcggcaatga cggccctgtc ggcgcactgg gcacgccgct
    13681 gatgggggaa tatgccggcg cagtcgaccg gcactacatt accttgggtg cgcccttatt
    13741 tgtcgccacc gcccatccgg ttacccgcaa agccctcaac cgcctgatta tggcgcagga
    13801 taccggcagc gcgattaaag gcgcggtgcg cgtggattat ttttggggtt acggcgacga
    13861 agccggcgaa cttgccggca aacagaaaac cacgggatat gtctggcagc ttctgcccaa
    13921 cggtatgaag cccgaatacc gcccgtaaac cggaaaacaa tgccgtctga agcccatccc
    13981 taggcaatcg ggcgcattaa ggcgaagttt tgcgataccg caggcacaaa attcctttct
    14041 tatttgacgg aattatagtg gatcaaatt
//
LOCUS       DAWEWP010000041        13506 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWEWP010000041 DAWEWP010000000
VERSION     DAWEWP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.041"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(77..152)
                     /locus_tag="WGN53_000880"
     tRNA            complement(77..152)
                     /locus_tag="WGN53_000880"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Asn,seq:gtt)
     gene            complement(343..1095)
                     /locus_tag="WGN53_000881"
     CDS             complement(343..1095)
                     /locus_tag="WGN53_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ0139893.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            1406..1930
                     /locus_tag="WGN53_000882"
     CDS             1406..1930
                     /locus_tag="WGN53_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ0139894.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNAPPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHWVVADIPADVRRRN
                     AASLQLSRCANIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     TLCGTFSSASTAAA"
     gene            complement(2019..2298)
                     /locus_tag="WGN53_000883"
                     /pseudo
     CDS             complement(2019..2298)
                     /locus_tag="WGN53_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(2670..3815)
                     /gene="dapE"
                     /locus_tag="WGN53_000884"
     CDS             complement(2670..3815)
                     /gene="dapE"
                     /locus_tag="WGN53_000884"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ0139895.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            complement(3928..4548)
                     /locus_tag="WGN53_000885"
     CDS             complement(3928..4548)
                     /locus_tag="WGN53_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ0139896.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYRPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPITLPDYNRWLDNFDTEKYAAFDMWHGAESEYDDHRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(4582..5091)
                     /locus_tag="WGN53_000886"
     CDS             complement(4582..5091)
                     /locus_tag="WGN53_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0139897.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDAEAFKRFVAGYDVHLAIEEPLFDMGNAFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(5186..5737)
                     /gene="azu"
                     /locus_tag="WGN53_000887"
     CDS             complement(5186..5737)
                     /gene="azu"
                     /locus_tag="WGN53_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ0139898.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            complement(6018..6440)
                     /locus_tag="WGN53_000888"
     CDS             complement(6018..6440)
                     /locus_tag="WGN53_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ0139899.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACVEDGRLILLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAAKLEKRHPELAEALANLVRRHGA"
     gene            6571..9321
                     /gene="secA"
                     /locus_tag="WGN53_000889"
     CDS             6571..9321
                     /gene="secA"
                     /locus_tag="WGN53_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ0139900.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            9467..11239
                     /locus_tag="WGN53_000890"
     CDS             9467..11239
                     /locus_tag="WGN53_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0139901.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            11427..13355
                     /gene="rpoD"
                     /locus_tag="WGN53_000891"
     CDS             11427..13355
                     /gene="rpoD"
                     /locus_tag="WGN53_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ0139902.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         3441 a         3831 c         3344 g         2890 t
ORIGIN      
        1 ctatcttgcc gcattccgaa cgccgtacgt gcaaatatga aaaagcccga acattcaagt
       61 tcgggcttca aaattctggc tccccgacct gggctcgaac cagggacctg cggattaaca
      121 gtccgtcgct ctaccgactg agctatcggg gaatggggtg tattatagcg tccgaaaaaa
      181 atgtgtcaat ccttaatttt ggaaaaatgg gcgacaaaac gacaagcata tgaatcagaa
      241 agacattaag accgatgcct taaaaggatt gccgttgtat gaatttccac agccgtcatc
      301 acaccatatt taagcccgat gagccgttct gccctccccc gcttaaaaca atgccgtctg
      361 aacttcgccg cgttccaaag ccagtaaaaa ctgtttgcgg ttcaacccgc ccgcgtagcc
      421 ggtcagtttg ccgtcgctgc cgatgacgcg gtggcaggga atcaggatag atactttgtt
      481 ctgcccgttg gcggcggcaa cggcgcggac ggctttgggg ttgcccaaac gctgcgcctg
      541 ctccttgtag ctgcgcgttt cgccgtaagg aatcgccaag agcgcgtccc atgcctgctt
      601 ttgaaactcg gtgccaatct gctccaaagg cgtggcaaag gttttcagac gacccttgaa
      661 gtataagtcc aattcctgcc gcaaaagttg cgtccgctca tcctcccgaa acacaaaccg
      721 tccgcgcaag gctttttgga cggcggcaat ttcctgttcc aaatgcttct gtccgacaaa
      781 ctccagcaga cacaatccct tatttccgaa caccgccagc atctcgccca aaggcgtggc
      841 aatggcggca cacaccagct cgttcaagct gtcgggataa cgcacttcca acagacggat
      901 ggctcggcgg atacggacat attcttcagg cgcgcagccg atattgtccc aaaaatcccg
      961 atcgaactgc ccggcttcgc attccgtcag attgggatgc ggcataacgc cgcactcaaa
     1021 aacccgggtt tcgaggcaat ggcggatttc atcccatttt gacggcagat tgtttaagga
     1081 aggcagggta atcattcgtt tgctccgtat ctctatcatg gatttggcgg caaaatcccc
     1141 aattctcgtc attcccgcac gccgatgcag gaacgggcta tgacgtaaat cttgagggtt
     1201 gggttgcggc aatgcctaaa tatgcgatat ttctaaagcc tcagggaaag gaattaaaga
     1261 cttcagacga cctgcaaagg gtcgtctgaa accacgattt ttgcatttac gcattccggc
     1321 acatcatcca accgtttcgg cacattcctg ccgccgttga cagcctataa tgaatccact
     1381 tattcatcaa gcaaaggaat catctatgca aacccgcatc ctctccgccg tactgctggc
     1441 tttttcaacc gctgcctttg ccgggggcgc attcacgctg caattcgaca acccgtccga
     1501 agacggcggc ttcacgcaaa accagctttt gagtgcgcct tacggcttcg gctgttcggg
     1561 cggcaatgct ccgcccgcgc tgtcgtggaa aaatccgccc gccgggacaa aaagtttcgt
     1621 cctgaccgtt tacgataaag acgcgccgac cggactgggc tggatgcact gggtggtcgc
     1681 cgacattccc gccgatgtcc gccgccgcaa cgcggcctcg ctgcaattaa gccgctgcgc
     1741 caacatcgcc gacgaccagt ccgcagccat atcggcggta atcagtttgc ggatttgccg
     1801 catcaggttg acgccttcgt acacggcaaa accgatgccg tcatgctgca accacgccaa
     1861 cacgccgcaa agcgcggcct ccgccacatt gtgcggcact ttttcatccg ccagcaccgc
     1921 agccgcataa tcaaacgccg cgcccatccg cccgggatgc ggcagcttca ccgcatcgca
     1981 cactgcctgc gccgtcccgt attgtgcggc aaacctttct atggcttcct gttcgaaagc
     2041 aatccattgc gcctgataga ggtcgtctga atcgggaata ttgatgacgt caaacgtctg
     2101 cccgcctgcc aaggcgaccg cctcacccgc cgcaatcctc aattcgcgcc tcgctcaccg
     2161 cagcttcttg cttccgcgcc gcacgataag cacagccggt tcatataccg ccacgctgcg
     2221 gtacaaggcg gtatgatgtt gcacgatgcc gcccaaagca cccaatcgtt cgcgcgtatg
     2281 aaagtaattt gacggcacta ttatctccaa cacgcagggc cgcacataag gcgcagccct
     2341 gtttaccctg atttggaagg ggttacttcc caaaaataaa ccaaaatccc actgccccga
     2401 atggccgggt ttcaaccata ttccgtcggt gtgcctgcaa cttgactccc tcccctgtgg
     2461 gggagggttg gggagagggt aaaacggggc agatacagac aatatttccg ttaccgcccc
     2521 gatgccctct ccctaaccct ctcccacggg agagggaatg gattgccgtt gaaataaatc
     2581 gctctacata aaaaatcaat gtgttatctc aaacccacgt taggtctaag caaatggtcg
     2641 gacatccgta tccggcaagc aagctgcttt cagacggcat ttccagccaa caagcgcgcc
     2701 aatatcccct catacaccgc cgacagcttc ggaatatcgt ccagccgcac gttttcgttg
     2761 atttggtgga tggtcgcatt ggacgggcct aattcgatga gttcttttgc aatggctttg
     2821 atgaagcgtc cgtccgaagt gccgccggtg gtggacaatt cggcctcaat gccgcaggtt
     2881 tcggcaatgg ctgcgcgtgc cacgtcggtc agtttgcccg cttgggtcag aaagggctgc
     2941 cccgaacacg accactgtaa atcgtattgc acgccgtgtt tgtccaaaat ggcgtgaacg
     3001 cgttgtttca gccctgcttc ggtggactcg gtggagaagc ggaaattgaa tttgacgttc
     3061 agctcgcccg gaatgacgtt ggtcgcgcct gtaccgccgt tgatattgga aatttgaaag
     3121 ctggtcggcg gaaagtattc gttgccttcg tcccagactt cctgcgtcag ctctaacaag
     3181 gccggggcaa aagtgtggac gggattgatt gccaaatgcg gataggcaat atgtccttgc
     3241 ttgcctttta cggtcaggtt gcccgacagc gagccgcgcc ggccgttttt aatcatatcg
     3301 cccaatttgt ccacggctgt cggttcgccg acgatgcagt agtcgataag ctcgtcgcgc
     3361 gctttcaata catcgacgac tttggtcgtg ccgtccaacg cgtcgccctc ttcgtcggaa
     3421 gtaatcagaa gcgcaatgct gccttggtgg tcgggatgtt cggcaacgaa gcgttcacag
     3481 gcggtaacga agcaggcgat gctggttttc atgtctgccg cgccgcgccc gtataatctt
     3541 ccgtcgcgct cggccggttc gaacgggggc gaatcccatt tttcgacagg acctgtcggt
     3601 acaacgtcgg tatgccctgc aaaacagacg acgggagctt tcgtgccgcg tcgcaaccag
     3661 atgtttttgg tgtcgccgaa atggagttct tcagccgcaa aaccgatttt gtgcaggcgt
     3721 tcggcaagga gtttttggca atccctgtcg tcaggggtaa cggatggtcg ggaaatcagc
     3781 tctttggcaa gctctaggga ttgagtttcg gtcatatttg ttcacttttg aaattagacc
     3841 gtctgaaacg ttctgaatgt gattttcaga cggcatttag gttaggttgg catacggggt
     3901 gggtatttta cccatcagtc ttctgaatca tttgccgtgg caggcttcat aaagcggcag
     3961 caaatcttcc accgtttccg ctatccattt cgcgacatcc tgcctgccca aatcgtcgcg
     4021 ttcgatgtgt ttgccaatgc agaaaaagtc ttcgtcgttt tgcaactttc ggtcggactc
     4081 gttttgttgc gcgacggtgc ggtggtcgtc gtattcgctt tccgcgccgt gccacatatc
     4141 gaatgcggcg tatttttcag tgtcaaaatt atccagccat cggttgtaat cgggcagagt
     4201 aatcggcgac acgtcggctt tgtagcagtg ccaatccaag ctgacactca gacggcggcg
     4261 gttaagtagt atcgacagga tggcggcaga gtttttgtat tgggcgtatt tgaaataggc
     4321 aaagaaatgg gcgcgaacct gccagccgtt acaccagcgt tcgatgtgcg gcggcgcaaa
     4381 cggcgcaccc aattcggcgg caacctgctg aatcagctgc tgccatatct gccagttttc
     4441 cttgtagtcg gctttgattt gcggaatgct ttcaggccgg tattttttaa gctgggcaaa
     4501 ctggaaaaac gggatattga acaaatcgca acttttcggg gtcagcataa tatatccttg
     4561 agacgattgt ttcagacggc attatttgcg ccggcgcgcc gccatgattt cgccgatttc
     4621 ggtcagtttt tcttttggga taaaggcgtt gcccatatca aacagcggct cttcaatcgc
     4681 caaatgaaca tcatatcccg ccacaaaacg tttgaacgct tccgcatcgg gaacataagc
     4741 attgtctgct tcgagtttgg caaattcggc ggaaacagcc gcccagttgt cgtgcagccc
     4801 gatatgttgg cgcaaaagct cgtccacgct ttcttgggct tgcggcgcat attgcagcag
     4861 cagcgggaag aagttttctt cttcgtcttc atggtgcagc ggcgcggcaa cgttgaaata
     4921 ctgggcgatt tggcggatgg tttgcaaaac aatctgattg cagccgtttt cggcgatata
     4981 gtccgacagc atggcgactt gtccgcaaaa acggcgcact ttgccgtggc aggcatacag
     5041 catttcaatc ggttcggcaa aggtaacgct tttggtttca aacggattca tgttttcgtt
     5101 ctcaacgggc acttttcaag cagtcatttt ataataaaac agcctgcaca aagcaggctg
     5161 tccgtctttt gagactttaa gcggattaat cgaccaaagt cactttgccg ttcatcaaag
     5221 caccgtgacc cgggaaggtg caggcaaatt tgtagtcgcc gtcagccaat ttggcaggat
     5281 ccagagtcag ggaagactct tcgccgccgc cgatcagttt ggtgtgggca acaacgcgcg
     5341 catcgtcagg tttgacatag tcggtatcgg cagcacctac gccgtcttta aatacgccgt
     5401 ccatgtcttc agctttggca atcacaaggt tgtgacccat gctggctttg ggttgcgtac
     5461 cggtatgttt cagagtgatg gtaaactctt tacatgcttt gctgacttgg atgtctttgg
     5521 tgttgaactg catattgtcg ttggattcga cagttgccgc acaattgccg gcagcagggg
     5581 cttcggcagc atctgcagga gcagcttcgg cggcaggcgc ttcggaagcg ggtgcttcag
     5641 cagcaggagt tgcctcggca gcaggcgcgg caggttcttg agagcaggca gccaaaccga
     5701 taacggcggc agaaatcaga gccagataag ctttcatggt aaatctccaa tcgataaaat
     5761 aatattcggt tttacagaaa tcaaagtgca accgccatta acaaaacctt gaaaaagatt
     5821 ccgccgcgtt gcacaaacag atgtttcggg gcggcatttt gctacaaatt tcatttgaaa
     5881 tcaaagcctg tctgcaagtt tacaatcgtt tactttaaaa ggggcaattt taccccgaac
     5941 ctatttcttt agtattagac ccattatcct ttacttctta atattaacgg atgtttacac
     6001 aaattcccgt atacatttta tgcgccatgc cttctaacca agtttgccaa tgcctccgcc
     6061 aattcgggat gccgtttttc caactttgcc gccgccgaac cgaaactctc cagcgcagcc
     6121 ttactcaaat gcagggtatt ggttttcggc ggtttttccg gtttcgggac cagcctgacc
     6181 gaaacagagc gtatcgaagc gtcaagccct gccagctgcg gcaataccga cggggcaatc
     6241 atcttcaagc gcgatgccgc catattgttt gccgccaaaa gaatcagcct gccgtcttcg
     6301 acgcaggccg tctgaaaatg cgggtgcagg ttggcaggca gcagtttttt cacggcggca
     6361 tccaaccgcc gccactgtcc cgcctgtttc aaaagtccgg aaagcagcgc gtcccgcctg
     6421 cccaactgtt ccaaattcat aaaacataca cccaaaaaga ttgaaatacc gcaaacgcgc
     6481 ctttatttca gacggcatta gcactttgca caaacgcttg tgttaaaatc gcgttttcgc
     6541 ccactattat atcaggcgca ggaattattc atgctgacaa acattgccaa gaaaatcttc
     6601 ggcagccgca acgaccgctt gctgaaacaa taccgtaaat ccgttgccag aatcaacgcg
     6661 ctcgaagaac agatgcaagc cctaagcgat gctgatctgc aagccaaaac tgccgaattc
     6721 aaacaacgcc tcgccgacgg tcagactttg gacggcattt tgcccgaagc cttcgccgtc
     6781 tgccgcgaag cgtcccgccg caccctcggt atgcgccact tcgacgtgca gcttatcggc
     6841 ggtatggtgc tgcacgacgg caaaatcgcc gaaatgcgta ccggcgaagg caaaaccctg
     6901 gtcgccaccc tcgccgtcta tctcaacgcg ctggccggca aaggcgtgca cgtcgttacc
     6961 gtcaacgact acctcgcctc gcgcgacgca ggcattatgg agccgctcta caatttcctc
     7021 ggcctgaccg tcggcgtgat tatttcagat atgcaaccgt tcgaccgtca aaacgcctat
     7081 gccgccgata tcacctacgg caccaataat gaattcggct tcgactacct gcgcgacaat
     7141 atggttaccg accaatacga caaagtgcag cgcgaattga attttgccgt tgtcgatgaa
     7201 gtggattcca tcttgattga cgaagcgcgc actccgctga ttatctccgg ccaggcagac
     7261 gacaacatcc agttgtacca aatcatgaac accgtcccgc cccacctcgt ccgccaagaa
     7321 accgaagaag gcgaaggcga ctactgggta gatgaaaagg cacatcaggt catcctgagc
     7381 gaagcaggtc acgaacacgc cgagcaaatc ctgacccaaa tgggattgct ggcagaaaac
     7441 gactcgctct attccgccgc caatatcgcc ctgatgcacc accttatggc ggcattgcgc
     7501 gcgcattccc tcttccacaa agaccaacat tacgtcatcc aagacggcga aatcgtcatc
     7561 gtggacgaat tcaccggccg gctgatgtcc ggccgccgct ggtcggaggg tctgcatcaa
     7621 gccgtcgaag ccaaagaagg cgtggaaatc aaacgcgaaa accaaaccct tgcctccatc
     7681 accttccaaa actatttccg cctgtacacc aagctctccg gcatgaccgg tacggcagat
     7741 accgaggctt tcgaattcca aagcatctac aacctcgaaa ccgtcatcat cccgaccaac
     7801 cgccccgtac agcgcaaaga tttcaacgac cagattttcc gttccgccga agaaaaattc
     7861 gaagccgtcg tcaaagacat cgaggaatgc cacaaacgcg ggcagcccgt cctcgtcggc
     7921 accaccagca tcgaaaactc cgaactggtc tcccaccttc tgcaaaaagc cggactgccg
     7981 cacaacgtcc tcaacgccaa agaacacgaa cgcgaagccc tgattgtcgc ccaagccggc
     8041 aaagtcggcg cgattaccgt tgccaccaat atggcgggac gcggtacgga catcgtttta
     8101 ggcggcaacc tgaagcacca aaccgatgcc atccgtgccg acgaaacctt gagcgacgaa
     8161 gagaaacagg cacaaatcgc cgcactcgaa gacggctggc aggcagaaca cgataaagta
     8221 atggaagcag gcggcctgca catcatcggt acggaacgcc acgaaagccg ccgtatcgac
     8281 aaccaattgc gcggacgttc cggccgtcag ggcgaccccg gatccagccg tttctacctt
     8341 tcgtttgaag acccgctgct gcgcttattc gcactcgacc gcgccgccgc catcctcaac
     8401 cgtctcgccc ccgaacgcgg cgttgccatc gaacacaacc tgctgacgcg ccaaatcgaa
     8461 ggggcgcaac gcaaagtcga aggcagaaac ttcgatatgc gcaaacaggt tttggaatac
     8521 gacgacgttg ccaacgaaca gcgcaaagtc atttacagcc agcgcaacga aattctgacc
     8581 agcaaagaca tcagcgacct gatgcaggaa atccgttctg atgtcgtcag cgacctcgtg
     8641 gatacctata tgcctcccga cagcatggaa gaacaatggg acatcccgac tttggagaac
     8701 cgtctggctg ccgaattcag actgcacgaa gacatccaat cctggctgaa ggcggacaat
     8761 gcgattgacg gtcaagacat caaagaacgc ctgatcgaac gcatcgaaaa cgaatatgcc
     8821 gccaaaaccg aactggtcgg taagcaggca atggccgatt tcgagcgcaa cgtgatgttg
     8881 caggtcatcg acaaccaatg gcgcgaacac ctcgccgcta tggactacct gcgacaaggc
     8941 atacacctgc gcagctatgc ccaaaaaaat ccgaagcagg aatataaacg tgaagccttt
     9001 accatgttcc aagacctgtg gaacggcatc aaattccata ttgcctccct gcttacctcg
     9061 gttcaaatcg aacaaaaccc tgtcgcggtg gttgaagagc aacccatcgg caacatccag
     9121 tccatccatt ccgaatcgcc cgatatggaa gaacttttgg gtcagtcgca aaccgatttg
     9181 gttaccgagg cctttaatcc cgatggaacg gatttcagcc ccgaagcctt ggaagcgcgg
     9241 ggacaaatcg tccaccgcaa cgacccctgc ccctgcggca gcggtttgaa atacaaacaa
     9301 tgccacggca aactggctta agcgtttgaa cgcaaatgcc gtctgaacat ccgatttccg
     9361 gcttcagacg gcattttgcc tgaaccgcca catccgactg ccattccgaa aaatcccgat
     9421 ttcgtaccgt ccgtaccaaa aacagacatc ccgtccgccc cacatcatga ttccatccga
     9481 cttcattgac gagcttttag ccaaaaccga tattgtcgat attatcgacg agcaggttcc
     9541 gctgaaaaaa ggcggggcga actatatggc gtgttgcccg ttccacaagg aaaaaacgcc
     9601 gtcgttttcg gtcagtccaa ccaagcagtt ttaccattgt ttcagttgcg gggcgcatgg
     9661 ttcggcgatt ggttttgtga tggagcatca ggggctgtcg tttccggagg ctgtgcagtt
     9721 tcttgccgac cgtgtgggca tggttgtgcc taaagtgcgt gggcagaacg ataatcccga
     9781 agtccgtgcc gaacgtaaga aaaaacagca gacgctggaa gaaacgacgg cggcggcggc
     9841 tgatttttac gcgcaacagc taaaattcaa tccggcggca aaagcttatt tggacaagcg
     9901 cggcttgagt gcagaagtta tcgcgcatta tggtttgggc tacgcgcccg acggctggca
     9961 gcctttggcg caagtgttcc aaccgtatcc gaataccgcg ttggtggata cggggatggt
    10021 gattgacaat gaggggcggt attacgaccg cttccgccat cggattatgt tccctatccg
    10081 caatccgcgc gggcaggtca tcggtttcgg cggcagggtg ctggacgact ccaaacccaa
    10141 atatctaaac tctcccgata cacctttgtt tgacaaaggg aaaaaccttt acggcttgta
    10201 tgaggggcgt gccgctgtca aggaagcgga gcggattctg gtggtcgaag gctatatgga
    10261 cgtggtcgcg ctggcacagt tcggcgtggg ctacggtgtg gcggccttgg gcacggcaac
    10321 aacagctgaa cacgtcaaaa tcctaatgcg tcaggcagac agtatttatt tctgtttcga
    10381 cggcgacagc gcggggcgaa aagcggcttg gcgcgcgctg gaaaacgcgt tgccgcagtt
    10441 gaaagacgac aaatcgctgc attttttgtt cctgcccgag gaacacgacc ccgacagtta
    10501 catccgtgcc tacggcaaag cgcaatttga agacgcgctt ctgaatcaaa gcaagccttt
    10561 gtcggagtat ttctgggaac acctttcaga cggcattcat ctcaatacgc aggaaggcaa
    10621 ggcggaattg gtgaaaacca gttcgccgct tttggcgcag attaccgcgc cggcattggc
    10681 ttatttgtta aaacaacggc ttagcgaact ggtcggcatc gaccccgaca acctcgcgca
    10741 actgctagga caggaagcgc cgaagcggca cgtcaaacaa aaaaactaca aactgcctcc
    10801 gatttccgtc aaacagcccg tcatgctgac gctggtacag cggcaaatcc gcagcctctt
    10861 gataaatccg gattgggctg catatataga cctgcccgat tatctggcgt tggacggtga
    10921 tttcgcctgc cttgccaatc ttgccgaatc gattaaaaac catgccgccg tacccgaaac
    10981 cgctcaggtt ttagagtata tgcgcggctc gccttacgaa gaaacgataa cccgaatctt
    11041 ccattcaacg caccaatcgg aagaaatgaa cagcagcagt gaagaagatt gcgagaattt
    11101 ccaaatcggc atgaaaaaac tgctcaatga gttaaaatac agccaaatcg aagcattgaa
    11161 acaaaaaagc ctgcaatccg gcttaaatga aagcgagaaa aaacttttgc tgtcgttgct
    11221 gaccgcaaaa caaaattgac cggcggattc cgccacccgt aaacccgtta tgccgtctga
    11281 aaagcactta ccccggctgc aacaacgaca cctgcagaac acccatcccc aaaagccttc
    11341 agacggcatc agagtaccct actctgccac gccttcaggt gcgtccaaac gcaaaccgtc
    11401 ggcatcttgc caacagaaag cagacaatgt ccaaaaacca aaatcacgaa gaataccaag
    11461 acgatgcccg acccctgacg attgaggaac aacgcgcgcg cctgcgccaa ctcatcatta
    11521 tgggcaaaga acgcggctac atcacctact ccgaaatcaa tgacgcactg cccgacgata
    11581 tgtccgatgc cgatcaaatc gacaacatcg tcagtatgat ttccggtttg ggtatccaag
    11641 ttaccgaaca cgcccccgat gcggaagaca tattgttaag cgacaatgcc gccgttaccg
    11701 acgatgatgc cgtcgaagaa gccgaggccg ccctttccag cgccgactcc gagttcggca
    11761 ggacgaccga ccccgtccgt atgtatatgc gcgaaatggg acaggtcgac ctgctgaccc
    11821 gcgaagacga aatcatcatc gccaaaaaaa tcgaaaacgc cctgaaaaat atggttcagg
    11881 ccatctccgc ctgcccggga tccattgcgg aaatcttaga actcatcgaa aaaatccgca
    11941 aagacgaaat ccgcgtcgac gaagtcgtag aagccattat cgacccgaat gaagtattgc
    12001 tcaacgaatt gggcttgggg cacttggaaa ccacagcgcc cgagaaacct tccaacgaca
    12061 attcggacga aaacgaagac gacgaagaat cggaagaaga tgcggatgaa atctcggcag
    12121 ccaatctcgc cgaattgaaa caaaaagtca tcggccactt tgcccaaatc gaaaaagact
    12181 acaaaaaaat gatcggtcgt ttggaaaaac accacagccg gcacaaagac tatctcgcct
    12241 accgcgacgc gattgccaac aaactgctgg aagtccgttt cgccacccgg caaatcgaca
    12301 gcctcagcag cagcctgcgc ggaaaagtag aaaacatccg caaactcgaa cgcgaaatcc
    12361 gcgacatctg cctcgaccgc gtccatatgg agcgcgacta cttcatccaa aacttcctgc
    12421 ccgaaatcac caatctggaa tggattgaag aagaaatcgc caaaggcagg gtttggagcg
    12481 acgcgctcga ccgcttccgc cacgccatcc tcgaaaaaca aaccgagttg gcagatatgg
    12541 aaaaagaaac ccgcatttcc atcgaagagt tgaaagaaat caacaaaaat atggtgtcga
    12601 gcgaaaaaga aaccgcagcc gccaaacagg aaatgattca ggcaaacttg cgcctcgtca
    12661 tttccattgc caaaaaatac accaaccgtg gcttgcagtt ccttgatttg attcaggaag
    12721 gtaacatcgg cctgatgaaa gcggtcgaca agttcgaata ccgccggggc tacaaattct
    12781 ccacctacgc aacctggtgg atccgccagg caatcacccg ttcgattgcc gatcaggcgc
    12841 gtaccatccg cattccggta catatgattg aaaccatcaa caagatgaac cgcatctcgc
    12901 gccaacacct tcaagaaacc ggcgaagaac ccgattccgc caaacttgcc gaactgatgc
    12961 agatgcccga agacaaaatc cgcaaaatca tgaaaatcgc caaagagccg atttcgatgg
    13021 aaacccccat cggcgacgac gacgattcgc acttgggcga cttcatcgaa gatgccaaca
    13081 atgttgcgcc ggccgatgcg gcaatgtaca ccagcctgca cgaagtaacc aaagaaatcc
    13141 tcgaaagcct gacaccgcgt gaggcaaaag tcctgcgtat gcgtttcggc atcgatatga
    13201 acaccgacca cacgctggaa gaagtcggca gacagtttga cgtaacgcgc gaacgcatcc
    13261 gacaaatcga ggcaaaagca ctccgcaagc tgcggcatcc gacaagaagc gaccgtttga
    13321 gaagtttctt ggacagcgaa gacagcaagc tgtaaaccaa aaaaccgcag gtttcaaata
    13381 cctgcggttt tttcttacac aataaacaac gcttccacat atcccacact cctatcccga
    13441 gacctttgca aaatccccca aaatccccta aattcccacc aagacattta ggagcacctt
    13501 cttcca
//
LOCUS       DAWEWP010000042        13390 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWEWP010000042 DAWEWP010000000
VERSION     DAWEWP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13390
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.042"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(111..539)
                     /locus_tag="WGN53_000892"
     CDS             complement(111..539)
                     /locus_tag="WGN53_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ0139903.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(604..2352)
                     /locus_tag="WGN53_000893"
     CDS             complement(604..2352)
                     /locus_tag="WGN53_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ0139904.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPPAGDFGARFKQNTDHIVLTHVFNSGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2679..4112
                     /gene="ccoN"
                     /locus_tag="WGN53_000894"
     CDS             2679..4112
                     /gene="ccoN"
                     /locus_tag="WGN53_000894"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ0139905.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4139..4750
                     /gene="ccoO"
                     /locus_tag="WGN53_000895"
     CDS             4139..4750
                     /gene="ccoO"
                     /locus_tag="WGN53_000895"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ0139906.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4755..4925
                     /locus_tag="WGN53_000896"
     CDS             4755..4925
                     /locus_tag="WGN53_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ0139907.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4949..6046
                     /gene="ccoP"
                     /locus_tag="WGN53_000897"
     CDS             4949..6046
                     /gene="ccoP"
                     /locus_tag="WGN53_000897"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ0139908.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6086..6280
                     /locus_tag="WGN53_000898"
     CDS             6086..6280
                     /locus_tag="WGN53_000898"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0139909.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6482..7891
                     /locus_tag="WGN53_000899"
     CDS             6482..7891
                     /locus_tag="WGN53_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0139910.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7992..11201
                     /gene="recC"
                     /locus_tag="WGN53_000900"
     CDS             7992..11201
                     /gene="recC"
                     /locus_tag="WGN53_000900"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ0139911.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPHIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGAGAA"
     gene            complement(11271..12839)
                     /gene="mtrF"
                     /locus_tag="WGN53_000901"
     CDS             complement(11271..12839)
                     /gene="mtrF"
                     /locus_tag="WGN53_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ0139912.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFELSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13173..13385
                     /locus_tag="WGN53_000902"
     CDS             13173..13385
                     /locus_tag="WGN53_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139913.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIARDVSLTTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3355 a         3235 c         3550 g         3250 t
ORIGIN      
        1 cactgtaaaa acggagaagg acagaggacg gcagtaaggt ttgccgtcct ctttttcaag
       61 ttgcggtgga atgccgcttg aaggtgtggt ttaaacgttt cggcttgtaa tcagacgcgg
      121 gcgttgtcca gccgctctct gaagttttcc aaatttacgc ctgccgcttg ggcgcggcgg
      181 atgatttcgt caaccggcat accttctgcc gcccggcgga agccccacag gagggagcag
      241 cgcgtaccgg tccggcaata ggcaaggacg ggatattcgg cttgtccgat gagttggcgg
      301 aaggtttcga catcgtgttt ttggatgtcg cgtgcggtaa cgggttggtg atggaatcca
      361 gtaacgcctg cttgttccag ccactgtttg atttgggcga agtcgggttg cgattcttct
      421 tcgcggtcgg ggcggttgca gatgacggtt ttgatgccca gttgcgcgat ttgttccgca
      481 tcggctttgg tcagttgcgg agaaatatag aggtgttcgt caagtttcag aatggccatt
      541 ttgtttcctt tactttgcgg ttgtttttca gacggcattt tgtgtgcaat gccgtctgaa
      601 agtttaaccg aacaaccagt tgtccaacag gttccggtct gtgatatgta ggatggcagt
      661 atccgctgcc gctgcctgaa ccgtcaagac ggtttgacgc aatatgccgg cacggaagaa
      721 gtggatattg atttttgcat tgacgtggta tcgggcccat tgtgcggtaa agtcggtgca
      781 ggcataaccg tctaaagcaa tgattttgtc ttgcgggcac agtgccgcag attccgcgct
      841 gccgctgttg aagacatggg tcaggacgat gtggtcggtg ttttgtttga aacgtgcgcc
      901 aaaatcgcct gccggcggga cggggcagat gtgttctgcg tatccgccgc cgtgttgtcg
      961 gggaagtggc aggaaggtca gtcccacgcc tgcggttgcc aggcattcgg caagcggcaa
     1021 atcttcggta ctgtataacg ctttttggaa aaaatcttcc aaatccaagc cggtaatttc
     1081 ctgacagcgg atttgccagt gtttttccgg aatacccgag tgtgtgtccc tccactcccg
     1141 atagagtttg tccattaacg tatcgagaga atgtctgccg ttgcttcggt tgcgtattat
     1201 cagatcaagg cacaatgcgg caagcgcgcc tttctggtag tagctgacga tggcgttggg
     1261 gctgttttca tccggtttgt aaaatttgtt ccacgcggta aaactcgatt ccgccaaggt
     1321 ctgcctcaaa cggccgcggg tttgttgtac gcgcgtaatg ccttgtgcca gcaggtttaa
     1381 ataagattcg ggcgagatgg tgcggctgcg tgccaaaaac aaatcgtcgt aataggatgt
     1441 aataccttcg aatgcccata gttgttcggt atagttttct ttgtcgaggt cataagggac
     1501 gaacgcggca ggtttgatgg atttgacgtt ccacgcgtga aaatattcgt gggagaaaag
     1561 tccgagcaat gtggtgtagg tatcgtcggc atcggtcata ccgtacggcg gaaggctgtg
     1621 gcggtcggcg agcagggcgg tgctgtcggt gtgttccaaa ccgccgtaaa tatggtcgcc
     1681 gacgtggagc aggaacaaat atttttgaaa cggggcaggg gaggaaaaca ccgccagttc
     1741 tgtttcgcag atttttttga tatccgaaac cagcctgttg cggtcgaaat cgggatatat
     1801 gccgcttaag gcaattgtgt gcggaatgcc tgccgcctca aaatctaaaa attcaatcaa
     1861 gcccatctcg acaggtcggt caatcaattc ggcataagat gccgcctgaa agacaaacct
     1921 ccctgtttcc ggcagcgttg tggcaatacg ccattcggac ggaatacccg tcaattccaa
     1981 gcggtgcggc agcgtttccg ttccttcgac tttcaaaaac aggcacgatc cgtcaaaaaa
     2041 accgcgttct gtcgtcagga aagaacctcg aaccgacaaa tcgaatgcat atacggtgta
     2101 gcggatttgc cactcgccgc gtacggcggc agcgtgccag cggttttttg caatttgttc
     2161 gagcggcatg gacgtgccgt tacaggatgc atggatagaa gtgatgtggc gggaaaaatc
     2221 ccgaatcaga tagctgcccg gtacccaatt cggcaggctt atttctatag gaagatcatc
     2281 atcttgtgtg aatgtcagca ggatgtgcca ctcgtggtcg agaggggacg gggttatttt
     2341 ataatgaatc ataactatca aatatatcgt taaacgggat ggattaaata cctttaaaat
     2401 gcagcttaac ctacatcaat aaagtgccac aaaaagggaa aattggaaac aaaagcaatg
     2461 gcggtcgggt tgtaatttat tataacaata tgatttatat gtaataaaaa acagattaag
     2521 caaagggctc gtcggcggcg cgaatgtaat tccgcttggc gtaataaggc agatatgtta
     2581 aatttagaaa aaattacgct tgcccggatt gcaccttaaa caggctgcgg aagcggcagg
     2641 ataaaaatct ttattcccat aaattcatgg agactctcat ggacacacaa acttacaact
     2701 acaaagtggt gcgccagttc gccatcatga ctgtagtttg ggggattgtg ggtatgttgg
     2761 tcggcgttat cgtcgccgcc cagctttttg ctcctgccct cgacttgtct aatatcggac
     2821 cttggttcca cttcggccgc ctgcgtccgc tgcacaccaa tgcggttatt ttcgcatttg
     2881 gcggttgtgg cctgatcggc acatcatact acgttgtcca acgtacttgt aatacccgtc
     2941 ttttcggcgg ttggctgccg gcatttacct tttggggctg gcaggcggta atcgttgccg
     3001 ccgtcgtcag cttccctatg ggttggaccc aaggtaagga atatgccgaa ctggaatggc
     3061 cgatcgatat tctgattact ttggtttggg tggcttacgc catcgtattc ttcggtacga
     3121 ttgccaaacg taagattaaa catatttacg ttgccaactg gttctacggc ggctttattt
     3181 tggcggtcgc acttttacac atcgtcaaca atatcagcat cccggccggt ttgatgaagt
     3241 cataccccgt ctattccggt gcgattgatg ctatggttca atggtggtac gggcataacg
     3301 ccgtgggctt cttcctgact gccggcttct tgggtatgat gtactatttc gtacccaaac
     3361 aagcagcccg ccccgtttac tcctaccgcc tgtccgtcgt tcacttctgg gcgttgattt
     3421 tcacctatat gtgggcgggt ccgcaccatc ttcactacac tgcgctgcct gactggacgc
     3481 aatctttggg tatggttctg tctttgattc tgttcgcacc ctcttggggc ggtatgatta
     3541 acggcatcat gaccttgtcc ggcgcgtggg acaaactgcg tacagacccg attcttaaat
     3601 tcctgattgt atccttgtcc ttctacggta tgtctacctt tgaaggcccg atgatgtcga
     3661 ttaaaacggt caatgcattg agccactata cggactggac cgtcgcgcac gttcatgcgg
     3721 gtgcgttggg ctgggtaggc tttgtaacca tcggttccgt ctattacatg attccccgtc
     3781 tgttcggcaa agaacagatg cacagcacca agctggtaga agcacatttt tggattgcga
     3841 ccatcggcgt ggttctgtat atcgctgcca tgtggattgc cggtgtgatg cagggtttga
     3901 tgtggagttc tttgaacgat gacggtacgc tgacctactc gtttgtcgaa tccgtaaaac
     3961 gcaccatgcc ttactacgtg atccgttttg caggtggttt attgtatctg agtggtatgt
     4021 gcattatggc gtacaacgtg taccgcacag ccatcggtgg taaagcagtc gatgccgaaa
     4081 tccctgcggt ttcccaaaca cagcaccact aaaatactaa gaaagatagg ctaccaaaat
     4141 gaaattacaa caattggctg aagaaaaaat cggcgttctg attgtgttca cgctgcttgt
     4201 agtcagtgtc ggtctgttga ttgaagttgt gcccttggcc tttaccaagg cggcaacaca
     4261 gccggcaccg ggcgtgaagc cttacaatgc cttgcaggtt gccggacgcg atatttacat
     4321 ccgtgagggc tgttacaact gccactctca aatgattcgt ccgttccgtg cggaaaccga
     4381 gcgttacggt cattactctg ttgccggaga gtcggtttac gaccatcctt tccaatgggg
     4441 ttccaaacgt accggtcctg atttggcacg tgtaggcggc cgctattccg acgaatggca
     4501 ccgtatccac ctgctgaatc cccgtgatgt cgtgcctgag tccaatatgc cggcattccc
     4561 atggcttgca cgcaataaag tcgatgtcga tgcaaccgtt gccaacatga aggctttgcg
     4621 taaagtaggt actccttaca gtgatgagga aattgcgaaa gcgcctgagg ctttggcaaa
     4681 caaatccgag ctggatgctg tagtcgccta tctgcaagga ttgggtctgg ctttgaaaaa
     4741 cgtaaggtaa catcatggat attaacggta ttcgcgctct tttcacggta tggatcttta
     4801 tctgtttcct gttggtactc tatatcgtct tcaacaggcg gaataagaaa aactacgata
     4861 atgccgcaaa cagcattttt gatgaaaacc aagatgcgca agataagaaa agcgaaaacc
     4921 gttaatattg tgataacgga gcaaaacaat gaacacaaca tcccaattta ccagtaattt
     4981 ctggaatata tatattgcag ttattgtctt actgagcttt atcgctttgg cttggctgct
     5041 gctgtctcaa aatgttgtca aacgtccgaa gaagggcgaa gaagtacaaa ctacgggtca
     5101 tgagtgggac ggcattgccg aatacgacaa tccgctgccc cgctggtggt tttggctgtg
     5161 tgttttgacg tggctgttcg gtatcggtta tttggttatg tatccgggtg tcggcgacta
     5221 caaaggtctg ctgaaatgga ccagccataa ccaatatgaa aaagaggtca aaaaagccga
     5281 tgagcaatac ggcaaactgt atgccaagtt tgcggatatg ccgattgaaa aagtggcaaa
     5341 agaccctcaa gccaagcaaa tcgcccaaaa cctgtttaac acttattgta tccagtgcca
     5401 cggctctgat gctaaaggct ctaaaggttt tccgaatctg accgatagcg attggttgtg
     5461 gggcggtgat cccgataaaa tccacgagac catcgaaaaa ggccgtgttg cgactatgcc
     5521 tgcctggggt cctgctttgg gcgaagaagg cgtgaaagac gttgcccatt atgtgatgtc
     5581 cctttcaaaa cccgaaggtc agtatgatga ggaacgtgcc gcgcgcggac aagccttgtt
     5641 cagcggtccg cctgccaact gtttcacttg ccacggcgat aagggacaag gtatccaagg
     5701 tttgggtccg aacctgactg atgacgtgtg gttgtggggc ggtacgcaaa aatccattat
     5761 cgaaaccatt accaacggtc gcagcagcca aatgcccgct tggggacatt tcttggataa
     5821 agacaaactg catattatga ctgcttatgt atggggtctt tccgataaag acggtaaagc
     5881 tccggtgaaa aaagccgagc ctgcaccgac tcccgcaccg gcggcagaac ccgcagcctc
     5941 tgctcctgca gaagcagcac aagccgcgtc cgaagccaaa cctgccgcag cagaacctaa
     6001 agccgaggaa aaagctgcac ctgctgccaa agcggacggc aaataggttt atgaaaccgt
     6061 ttgtgccgcc tgccatggca atgcgattcc gggtattccc catgtaggca tcaaagccga
     6121 ttgggccgac cgcatcaaaa aaggcaagga cacgttgcac aaacacgcga tcgaaggttt
     6181 caatacgatg cccgcaaaag gcggtcgcgg cgatttgagc gatgatgagg ttaaagctgc
     6241 ggttgactat atggtcaacc agtcaggcgg caaattctaa cttgactgag tttccgatat
     6301 tatcggttat gaaaatccag attcagtatt gaatctggat tttttgtttt aacgggcatc
     6361 cggttttaaa cgcagactgc ctatttgttg taatgaattt tgactttagt cataaagaat
     6421 tagtctttat tgtttataat cgcgcatcat cacgatagcc ctattttcaa aaggacgatt
     6481 aatggataca caaatcaaaa ctgaagccga caatcagagc aaccggcgtt atctgaccgt
     6541 ttggcggtgg catttttatg ccggtctgct ggttatgcct tttcttaccc tgcttgccgt
     6601 tacgggtttg ggtatgctgc tgtttgccaa tattaccggt aaggagggcg agcggattca
     6661 tgttgtgccg caggcaacgg tacaacctct gtctgttcag gcggaagcgg cacgcagtgc
     6721 cgttaatccg gaaacttcgt ccgtcgttca gtatattgcg ccgcgtgccg atgatatggt
     6781 tgccgtgttc cgtgtcaaca atgagggcaa ggcaacgatg gtcgcggtcg atccttatac
     6841 ggcaaaagtg gtcaacacca tgccgcgcaa tcagggttgg tatcacacga tggatgaaat
     6901 ccacagcgat atgatgctcg gttcgacagg cgattatctt ttggaaacgg ctgcatcgct
     6961 gacgattatc atgataatca gcggtttgta cctttggtgg gtgaaacggc gcggcatcaa
     7021 ggcgatgctg ctgccgtcaa aaggcagggc gcgttcttgg tggcggaatc tgcacggcac
     7081 gtttggaact tgggtgtcgt tgattttgct gttgttctgc ctgtcgggta ttgcttgggc
     7141 gggtatttgg ggcggcaagt tcgtacaggc ttggagtcag ttccctgccg gtaaatgggg
     7201 tgtcgaaccg aaccccgttt cagtcgtgcc gacccacggc gaggtattga atgacggcaa
     7261 ggttaaggaa gtgccgtggg ttttggagct tacgcctatg cctgtttcag ggacgactgt
     7321 gggcaaagac ggcattaacc ctgacgagcc gatgacattg gaaaccgtcg accgctttgc
     7381 gcgggaaatc ggtttcaaag ggcgttatca gttgaatttg cccaaaggcg aggacggcgt
     7441 atggactttg tcgcaggatt ctatgagtta cgacatgatc agcccgtttg ccgaccgcac
     7501 ggtacatatc gaccagtaca gcggcaaaat ccttgccgac atccgttttg acgattacaa
     7561 cccgttcggc aaatttatgg cggcaagcat tgcgctgcat atggggactc tgggctggtg
     7621 gagcgtgttg gcgaacgtct tgttctgcct tgccgtcatt tttatcggta tcagcggctg
     7681 cgtgatgtgg tggaaacgcc gtccgaccgg agcggtgggc atcgttccgc cggcgcagaa
     7741 agtcaagctg ccggtttggt ggatgatggc attgccgcta ttggcaatcg cactgctctt
     7801 cccgaccgca ctgcttgcca ttgccgtgat ttggctgttg gatacgctgc tgttgtcgcg
     7861 gattcctgtt ttgaggagat ggtttaaatg accaaatgcc gtctgaaagg ttcagacggc
     7921 attttgtttg aaggcggacg ggggaaaggc tatataatcc cgaatacttt tccgcaactt
     7981 ttgtcgaaat catgttttat ctgtatcaat ccaaccgtct tgaaacgctg gcggcattgt
     8041 ttgcccgcat tcaaaaagtc aaaccgctga aatcggcttt acagcccgaa cagattattg
     8101 tgcagagtca ggggatgcgc cgctatctca atacctgcct cgcccgcgat ttgggcgtgg
     8161 cggcgaattt gtcgttcagc ctgcccgccg gcctgacgtg gaagctgatg aaaaaactga
     8221 ttcccggtat tccggaactc agcccgtttg cacccgaagt catgcgctgg cggctgctgg
     8281 atttgttccg cagtgaagca ttccggaata cggcagaatt tgaagatgtg aggaatgtgc
     8341 tgcaagacta tctgggcagc ggcgaatcgg cagattacca gcttgcggga cagcttgcgg
     8401 acatattcga ccaatacctc gtgtaccgtc ctcagtggat agacgcttgg cagcagggca
     8461 ggcggctcgg tttgggcgac gacgaaatct ggcagtccaa actgtggcgt tacctcgacg
     8521 atggcaggca gagcgcgccg caccgtgtcg cgttgtggga aaagctgctt gccgcgttgg
     8581 acaaggataa gctgcccgag cgttatttcg tgttcggcat ttccacgatg gcgccgatgt
     8641 atcttcagct tttgcacaag ctgtccgaac attgcgatgt gttcgtgttc gcactcaatc
     8701 cgagcgggat gtattggggc aacgtcatcg aagcggcgca aatcctcaaa ggcggcggcg
     8761 atcccgattt aactcaggca gggcatccgc tgctcgcctc attgggcaag caggggcgcg
     8821 actttttcga ctttttgaac gaaatggaaa tagaaggaga aacgccggta tttgaagaag
     8881 gcgggcgcga tacgcttttg cacgccctgc aaaccgatat tcaaaacctg aaaatgccgt
     8941 ctgaaaatgt gggaagcgtc aacacgggcg acggctcgat acgcatcgtc tccgcacaca
     9001 gccccttgcg cgaattgcag atactcaaag acaagctgtt gaaaattctg catgaacatc
     9061 ccgattggca gccgcatgat atcgccgtat taaccccgca catcgaatcc tatacgcctt
     9121 ttatcgaagc cgtgttcgga caggcgcagc ccggtgcgca ggcattgccg tattccgttt
     9181 cagacgtgaa aatcagccgc cgacaaccgc tgtttcatgc tttgtcatgc ctgttcgact
     9241 tgttggaaag ccgatttgaa gtcgataaag tgcttgtgct tttagaaacc gcacccgtgt
     9301 tgcgccgttt cggactgact gaggacgatt tgccgctttt gcacgatatg gttgccgatt
     9361 tgaacgttca ctggggtttg gacggagaaa tgcgcggcgg cacggatcag ctgtttacct
     9421 ggaagcaggc ggtagaacgc atgatattgg gctggatgct gcccgaaggc ggcaacccga
     9481 tgtggcagga tgtcagcgcg tggtatgccg acgtgaacca aaccgccctg ttcggacgtt
     9541 ttgccgccct cctcgaaacc ctgtcggata ttgtacggat atggcggcag cccgcaacga
     9601 tcggcgaatg ggtggcgcgt tgccgggatt tgcttgaaac attgttccaa gctgaagccg
     9661 atgaccaaaa gtcagtccaa aaccttgaaa acgaatgggt caaatggcag gcggaaacag
     9721 aattggcgca attttccgga cagttgccgc aacaaaccgt catccgccat atccgacgtt
     9781 tcctcgacag cgaaagcgag gcaggctttt tacgcggcgg catcaccttt tgcagtatgg
     9841 tgccgatgcg gagcctgccg ttcaaagtca tctgcctgtt gggtttgaac gacggagatt
     9901 ttccccgcaa taccaaagcc gccgtattcg acctgattgc caaacatccc gccaaaggcg
     9961 accgcgcccg ccgcgatgac gaccgctacc tcttcctcga agccctcatc agcgcgcgcg
    10021 aaatcctcta cctgtcctac atcgggcgcg acatccgcaa agacgaagag cttgctccgt
    10081 cttcgctgtt gggcgaactc atcgacacag ttgccgctat ggcgggcatc ggtagccgcc
    10141 aacttgcaca aaactggata gaacagcatc cgctgcaagc cttctcgcgc cgatattttc
    10201 aagaaggcgg acgttcagac ggcatattcg gcacgcgtac cgactacgcc actgcgctcg
    10261 gacaaacgcc cgaaccgccg caaccctttt tcgaccaacc cgtagaaaac gccgaacctg
    10321 ttgccgaaat cggacaggac gaatttatcc gtttctggcg caaccccgtc aaagtatggc
    10381 ttcagcagca gcttgcgtgg agcgaacccc atatcggcga agcctgggag cctgccgaac
    10441 ccttcgagcc gcaacacgcc gatcaaatcg ccgaaaccta catcgaagca cggcgcgaag
    10501 gacgggattt tgcccaaacc gccgcccgca tcggggcgga aagcctcctg ccgtcgggag
    10561 agttgggcag actttggcag cagaacttcc aaactgctgc caaacaaatc gacacggcgg
    10621 ttttaaacag ccccaaactg ccaccgcttt catatgccat accgtcggac gggcaaatcc
    10681 tgaaaggcag cttgggcaat ctgtaccgct gcggacaagt gttttacgcc tacggcaaac
    10741 ccaacgcgcc gcaacgtatc gcttttctgc tggaacacct gatattttgc gccgttatgc
    10801 cgtctgaagc cgaaacgcgg caaaccttta tcgtccaatc cggagaaacc gaaatattgg
    10861 cggaaatcgc gcaagacagg gcattgcagc tattgtcgga atggatggcg tttttcaata
    10921 tcgggcaaaa ccgcccgctg ccgttttttg ccaagacctc gcttgccgct gccgaagcgt
    10981 ttgcccaaaa acaagattgg gaagccgccc tgaaaaaagc ccaaaccgcc tattacggca
    11041 acaaagtcag caaagggcag aaagactata ccgaagtcgc cctcgtgttc ggcaacgcaa
    11101 gccaaaaccc actcgaacaa cccctgtttg aaaacctcgc ccgcctgctt gccgacacgc
    11161 ttgccgcagc ggaaaaaagg gaaggggccg gagcagcctg acgggatgga aaggcaaatg
    11221 ccgtctgaaa acagcaaaaa ggttttcaga cggcattttg tttatcgtgt ttaaggtgcg
    11281 ggatagaatg tgggcgcgcc gggaccgacg ggcaggccca aaacaaatac ccaaatgcag
    11341 aataaggcaa tccacgcaat caagaagaaa gcggaatacg gcaacatcat agaaatcagc
    11401 gtacccacgc ccgcatcttt tttgtatttg atcaccgtcg ccataatcag cccgaaataa
    11461 ctcatcatcg gcgtaataat attggtaacg gaatcaccga tgcggtaagc ggcttgaatg
    11521 acttcgggcg cgtagccggc caacatcagc atagggacga aaatcggcgc agttaccgcc
    11581 cattgcgcgg aggcggagcc tatcatcaga ttgataaaag cacaaattaa aataaaaccg
    11641 ataaacaaca cgctgccgcc caagccgact tcttttaaga acgtcgcccc tttaacggca
    11701 atatattgcc caatattcgt ccaattaaaa aatgcgacaa actgtgcggc aaaaaagatg
    11761 atgaccaaat aaagccccag agtactcatc gattcggcca tcgcattaac gacttcctgt
    11821 tcgccgcgca aacttcgggt tacccggcca taaacaatgc ccggcagtgc aaacaacaag
    11881 aaaataaaaa caacaattga ttttaaaaac ggcgaaccgg aaaccaatcc tgtttcagga
    11941 tgacgcaaaa taccgtcggc agggacgatg ctccaagcca atagggcgga taaggcaaca
    12001 aacaccacgc cagcccaaat taatccttta tattccaaag gcgtgatttc attggaatgt
    12061 cgaatgtctt tttcttcttg tgacaaatct gattgataag ggcccaattg cggttcgacg
    12121 attttttcag taacaaaata accaatcaaa gcaatcacaa acgtactggc taccataaaa
    12181 aaccagttgg cttcagggcc tacgacgtag tcgggatgga tgatttgcgc cgcctgttgg
    12241 gtgatgcctg ccaagagcgg atcgattgtg cctaagaaca gattggccga ataaccgccc
    12301 gaaacgccgg cgaaagccgc agccagaccg gcaagcggat ggcggccgag ggaatgaaag
    12361 atgatggcgg acaaagggat taggacgaca tagcccaatt cagaagcggt attagataaa
    12421 atccctgtaa aaacaaccat aaaagtagtg agtttgcgtg gagattttgt gagcaataag
    12481 cgcattaatg cggaaatcaa gcccgatttt tccgcaatcc ccacgcccaa taaagaaacc
    12541 aacaccgttc ccaacggcgc gaaaccggtg aaatttttaa cggtatgcgt caggattttg
    12601 atcaaaccgt cagcatcgag caggctgaca acgtgaatca aaccgtcatc ggcacgtcct
    12661 ttcgcaccaa cagggcgcgg atcggggacg gatagttcga aatacgcacc ggcggcagag
    12721 gcaatcagca ataacacaat gaaaataata aaaagcgtaa ccgggtgcgg caacatattg
    12781 cccagccatt cgactgtgcg taaaaatcgt ccgtcccgtt gcgtatcggt ttgactcatc
    12841 tgcttctcct taaaaaaaat atattgagtg cattaaattt ttgaatgtaa catgaagtgt
    12901 tttgtaagca aactaaaata atcgtatatg tatataaccg taatagatta aaacagaaaa
    12961 tgcccacatc gttattctca taaaggcggt aatccgaaaa cttgacgtta aagccttatc
    13021 aggaatgact ggaactcaaa aaaacggatt cccgcctgcg cgggaatgac gggattttcg
    13081 gtttttgttt gcaagaatgc cggagaagaa tgcagtttac attttgaatg gtattgttat
    13141 taattgtaaa taaatattgg aaggtaaatg atatgaaaaa tgaaattcaa aaaatcatgg
    13201 acaaatataa tccatggcat gaagatgatt ttgagtcgta tgaagacatt gccagagatg
    13261 tatcgctgac gacagataaa acgttcattg aacattattt gttagaagtt tattcagaag
    13321 aaaacggaca ttttgaccaa gagaatgtcc atgcaatgat agaagaaatt aaaaatgcaa
    13381 tttagtgaat
//
LOCUS       DAWEWP010000043        13355 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWEWP010000043 DAWEWP010000000
VERSION     DAWEWP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.043"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(91..864)
                     /locus_tag="WGN53_000903"
     CDS             complement(91..864)
                     /locus_tag="WGN53_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ0139914.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSAAVRGKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
     gene            complement(963..1586)
                     /gene="rsmG"
                     /locus_tag="WGN53_000904"
     CDS             complement(963..1586)
                     /gene="rsmG"
                     /locus_tag="WGN53_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ0139915.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTGHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            complement(1682..2167)
                     /locus_tag="WGN53_000905"
     CDS             complement(1682..2167)
                     /locus_tag="WGN53_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139916.1"
                     /translation="MYVNEKYPYAALFAGLVFLTLPFALSVHDALAFAFGRAGLLVSV
                     SDGEFGWRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSAALGGLRGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            2272..2347
                     /locus_tag="WGN53_000906"
     tRNA            2272..2347
                     /locus_tag="WGN53_000906"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2305..2307,aa:Thr,seq:cgt)
     gene            2421..3548
                     /locus_tag="WGN53_000907"
     CDS             2421..3548
                     /locus_tag="WGN53_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ0139917.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            3729..4286
                     /gene="frr"
                     /locus_tag="WGN53_000908"
     CDS             3729..4286
                     /gene="frr"
                     /locus_tag="WGN53_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ0139918.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            4342..5088
                     /locus_tag="WGN53_000909"
     CDS             4342..5088
                     /locus_tag="WGN53_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ0139919.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            5092..5889
                     /locus_tag="WGN53_000910"
     CDS             5092..5889
                     /locus_tag="WGN53_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ0139920.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLVATLVFGVVAYAGGWMLPNLVWYVVLAFWLAIMPLWLRFKWRLN
                     GGWQVYAVGWLLIMPFWFALVSLRPHPDNALPLLAVMGLVWIADVFAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            5894..7129
                     /locus_tag="WGN53_000911"
     CDS             5894..7129
                     /locus_tag="WGN53_000911"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ0139921.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            7164..8504
                     /gene="rseP"
                     /locus_tag="WGN53_000912"
     CDS             7164..8504
                     /gene="rseP"
                     /locus_tag="WGN53_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ0139922.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            8561..10954
                     /gene="bamA"
                     /locus_tag="WGN53_000913"
     CDS             8561..10954
                     /gene="bamA"
                     /locus_tag="WGN53_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ0139923.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            11020..11520
                     /locus_tag="WGN53_000914"
     CDS             11020..11520
                     /locus_tag="WGN53_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ0139924.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            11553..12599
                     /gene="lpxD"
                     /locus_tag="WGN53_000915"
     CDS             11553..12599
                     /gene="lpxD"
                     /locus_tag="WGN53_000915"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ0139925.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPSATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            12633..13082
                     /gene="fabZ"
                     /locus_tag="WGN53_000916"
     CDS             12633..13082
                     /gene="fabZ"
                     /locus_tag="WGN53_000916"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ0139926.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVS
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            13247..>13355
                     /locus_tag="WGN53_000917"
     CDS             13247..>13355
                     /locus_tag="WGN53_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abi-like family protein"
                     /protein_id="HEZ0139927.1"
                     /translation="MDYKECCSIFSDFRMERYKNAVGEDKAAELYLLNLS"
BASE COUNT         3273 a         4185 c         3372 g         2525 t
ORIGIN      
        1 atgacgatat aagtattttt attttaatcc gccatattaa cgcacccggc caaacagcat
       61 aaaggcacgg gcagcccgat ttggattgac ctatttcccc gacaccctcg ccatcagctc
      121 gtccgccaag gcaagatacg ccttggtacc ctttgcctgc gcgtcgtaag ccatcaccgg
      181 cataccgtgg ctcggtgctt ccgcaaggcg gatattgcgc gggatgacgg tttcaaacag
      241 caaatccccg aaatggctgc gcaactgttc gctgacttcg gcaaccagcc tgctgcggct
      301 gtcgtacatc gtgcgcacga tgcccgtgat gtccaaatcg ggattgaccg cctgacggat
      361 tttacgcacg gtcgcaatca aatcggaaat cccctcaagc gcgtaatatt cgcacaacat
      421 cggcacaatc acgccgcccg ccgccaccaa gccgttaagc gtcaacagcg tcagcgaagg
      481 cggacagtcg atcaggataa agtcgtaatc ttccgccact gccttgagtg cgtttttcaa
      541 acgcacttcc cgggcgattt cctgcaccag ttcgatttcc gcgccggcaa gtgcgcggtt
      601 cgcacccaac acagcgtatc cgccctcttt gccgcgtacc gccgccgatt tcacgtccgc
      661 atcgcccaat aagacctgat aaacgccgga ctgcaaaccc gccttgtcga taccgctgcc
      721 cgtcgtcgca ttgccctgcg gatccaaatc gaccaccagc acgcgtttgc cgcgcgatgc
      781 cagcgaagcc gccaaattta ccgtcgtcgt cgttttgccc acaccgccct tctgattggc
      841 gatggcaagg atgttcgcac tcatgtcccg cccgtgtccc gtctgatgtt gaaaaaagaa
      901 aaacagtgcg gaattttacc tttatccgca caaaaaaggt attcatgccg tctgaagtgc
      961 gctcaacgct tgctcaggat gacgatatgg cgttccgcat ccaagcctgg cacgtcgagc
     1021 ctttggactt tttcaacgca cacatcctgc ggcaggcggc cgatttcccc ctgcggatac
     1081 acgcccttca tcgccgccca gtatccgccg tctttcaaca gatgccccgt ccacgacaca
     1141 aagtccgcca gttctgcaaa cgcacggctg gtaaccacat cggcacgcac gtccgaaacc
     1201 gcctccacgc gtccggatac cacgcgcaca ttgtccaacc ccaactcgat aaccgcctgc
     1261 tgtaaaaaag ccgttttctt cgtatttgca tccaaaaggg ttatctgcac atccggacgg
     1321 cacaccgccg ccggaatgcc gggctgaccg ccgcccgaac cgacatccag catcgtttgc
     1381 acaccctcga tatggggcag cagcgtcagg ctgtccaaaa gatgatggac aatcattttt
     1441 tcctcgtcgc gcagggcggt cagattgtag gttttgttcc actttttcaa caaatccaca
     1501 taggccaaaa gcctgtcctg cgccgtttcc gaaatatcca gccccatcgc ggcaatgcct
     1561 gcacgcaggc gttctttgcg ttccatatct gcttcctgtc catattcagg cgtaatgtta
     1621 gcggaaaaac atgccatctg aaaccgcgtg tcggggttca gacggcattt cccgttccaa
     1681 cctatttttt cctgtgaaac accaccggct gcaccggcgc gcgcgtcagg cgtttgggca
     1741 gcgtcagcat agacaccagc cacacggcaa acgcatattc cgacaccgcc tccccgcgca
     1801 gcccgcccaa ggcggcactg cccaacaaca gctcgaaaat ccacgcgacc gcagcagccg
     1861 cgcaagccgc cacgcccgac agaaacagcg cgtaacgcga atgccgccgc atcatctttt
     1921 tgtacgccag tttcgtcagg cccgccgaca caaccacatt caaaaacgca aacacaccga
     1981 acacaaacca aacagtgccg tcccaaccgc cacgccagcc gaattcgccg tccgacaccg
     2041 acaccagcag ccccgcccgt ccgaacgcaa atgccaaggc atcatgcacc gacagtgcga
     2101 acggcagcgt caaaaacacc agccccgcaa acaaagccgc atagggatat ttctcgttca
     2161 catacatcac cgactctcct cacgaagcaa accgcattat accgtgcaaa actttgccag
     2221 cagcccgaaa agttgcagaa acaaccgttc atatatatca taacgaaaaa agccggtgta
     2281 gctcagtcgg tagagcagcg cattcgtaac gcgaaggtcg ggggttcgat tcccttctcc
     2341 ggcaccaata ccaagcacag accctccctt cctcgggaag cctgtgcttt ttcacatttc
     2401 cgcttcagac ggcaaccgat atgaacacct cgcaacgcaa ccgcctcgtc agccgctggc
     2461 tcaactccta cgaacgctac cgccaccgcc gcctcataca cgccgtgcgg ctcggcgggg
     2521 ccgtcctgtt cgccaccgca ctcgcccggc tgctccacct ccaacacggc gaatggatag
     2581 gcatgaccgt cttcgtcgtc ctcggcatgc tccagtttca aggggcgatt tactccaagg
     2641 cggtggaacg tatgctcggc acggtcatcg ggctgggcgc gggtttgggc gttttatggc
     2701 tgaaccagca ttatttccac ggcaacctcc tcttctacct caccgtcggc acggcaagcg
     2761 cactggccgg ctgggcggcg gtcggcaaaa acggctacgt ccctatgctg gcggggctga
     2821 cgatgtgcat gctcatcggc gacaacggca gcgaatggtt cgacagcggc ctgatgcgcg
     2881 ccatgaacgt cctcatcggc gcggccatcg ccattgccgc cgccaaactg ctgccgctga
     2941 aatccacact gatgtggcgt ttcatgcttg ccgacaacct gaccgactgc agcaaaatga
     3001 ttgccgaaat cagcaacggc aggcgtatga cgcgcgaacg tttggagcag aatatggtta
     3061 aaatgcgcca aatcaacgca cgcatggtca aaagccgcag ccacctcgcc gccacatcgg
     3121 gcgaaagccg catcagcccc gccatgatgg aagccatgca gcacgcccac cgtaaaattg
     3181 tcaacaccac cgagctgctc ctgaccactg ccgccaagct gcaatctccc aaactcaacg
     3241 gcagcgaaat ccggctgctc gaccgccact tcacactgct ccaaaccgac ctgcaacaaa
     3301 ccgtcgccct tatcaacggc agacacgccc gccgcatccg catcgacacc gccatcaacc
     3361 ccgaactgga agccctcgcc gaacacctcc actaccaatg gcagggcttc ctctggctca
     3421 gcaccaatat gcgtcaggaa atttccgccc tcgtcatcct gctgcaacgc acccgccgca
     3481 aatggctgga tgcccacgaa cgccaacacc tgcgccaaag cctgcttgaa acacgggaac
     3541 acggctgacg gtcggacacg atgccgtctg aaacgcctgc cgcttcagac ggcatcccca
     3601 atccgcctgc cttgtcgtca tgcccgaata acggtactat tcgcagttaa cagttttcat
     3661 gccgtctgaa aggtttgaag cgcgtttcag acggctgccc gcccacctta tcccaccgaa
     3721 agaacaatat gatcaacgat attcaaaaaa cagccgaagg caaaatgcag cgttcggtcg
     3781 aagtactgaa agaaaatctg gcgaaagtgc gtaccggccg cgcgcatacc ggcctgctcg
     3841 accaagtgga agtcgaatac tggggcagca tggtccccgt cagccaagtt gccaacgtaa
     3901 cgcttctgga cgcgcgcacc atcggcgtga aaccgtttga gggcaatatg gcggccaaag
     3961 tcgagaaagc catccgcgat tcaaacttgg ggctgaaccc ggcagctgtc ggcgacctga
     4021 tccgcgtgcc gatgcccatg ctgaccgagg aacgccgcaa agacctgatt aaagtcgtac
     4081 gcggcgaagc ggaagaaggc cgcgtctcta tccgcaacgt gcgccgcgat gccaacgacc
     4141 acatcaaaaa actcctcaaa gacaaagaaa tttccgaaga cgaggcacgt cgcggcgaag
     4201 aagcggttca aaaactgacc gacaaataca ttaccgaagc cgacaaactc ctgactgcca
     4261 aagaagaaga tttgatggca atttaacctg cacggttcgg cgttcagacg gcatttgaat
     4321 gccgaaccgc gaaaggcaga catgaaaagc agcacgcagg ccgttttgga acacaccgcc
     4381 attcccaagc atatcgccgt gattatggac ggcaacggcc gttgggcgaa aaaacgtttc
     4441 ctcccgcgca taatgggaca caaacgcggt ttggacgcat tggaaaatat ggtgaagcat
     4501 tgcgccaaac tgggtgtgca atatctgacc gtgtttgcct tttcaaccga aaactggcgc
     4561 cgccccgaag acgaagtttc gttcctgatg gggctgtttt tacaggcttt gcaaaaacag
     4621 gtacgccgtc tgcacgaaaa caatatgcgc ctgaaaatac tgggcagccg cgaacgcttc
     4681 aaccggcaga ttctgcaagg catcgaagaa gcggaggcgt tgacggcaaa caataccggc
     4741 ctgaccctga gcattgccgc cgattacggc ggccgctggg atattttgca ggcggcaaac
     4801 aaactgattg ccgaaggcgt atccgagatt acagaagaca cgctggcgaa acacttgatg
     4861 ctgggcgatg caccggaacc ggatttgttc atccgcaccg gcggagaaac gcgcatcagc
     4921 aatttcctgc tctggcagat ggcgtatgca gaactgtatt tcaccgacat cctgtggcct
     4981 gatttcgacg aaaccgcctt agatgccgcc gtcgcttcgt tccaaaaacg cgaacggcgg
     5041 ttcggacgca cctccgagca actgcctatc gaacagcaaa ggaactaaat tatgctgaaa
     5101 caacgggtaa taaccgctat gtggctgctg ccgctgatgc tgggcatgct gttttacgcg
     5161 ccgcaatggt tgtgggctgc attttgcgga ctgattgccc tgattgcctt gtgggaatat
     5221 gcccgtatgg gcggcttgtg caaaatcaaa accaaccatt acctcgtcgc aaccttggtt
     5281 ttcggcgtgg ttgcctatgc gggcggctgg atgctgccta atttggtttg gtatgttgtt
     5341 ttggcatttt ggctcgccat catgccttta tggttgagat ttaaatggag gctcaacggc
     5401 ggttggcagg tttatgccgt cggctggctt ctgatcatgc cgttttggtt cgcgctcgta
     5461 tccctgcgcc cgcatcccga taatgccctg ccgctgctcg ccgtcatggg tttggtgtgg
     5521 attgccgatg ttttcgcgta tttcagcggc aaggcgttcg gcaaacacaa aatcgcaccg
     5581 gcaatcagcc ccggcaagag ctgggaaggc gcaatcggcg gcgcggtttg cgtggccgtg
     5641 tacatgaccg ccgtacgaag tgccggctgg ctggcattcg atacaggctg gttcgatacc
     5701 gtgttaatcg gtttggtgtt gaccgttgtc agcgtatgcg gcgacctttt ggaaagctgg
     5761 ctcaagcgcg cggcaggcat caaagacagc agcaagctac tgccaggaca cggcggcgtg
     5821 ttcgaccgta ccgacagcct gattgccgtt atcagcgtct atgcggcgat gatgtcggtt
     5881 ttaaattgat tctatgccgt ctgaaaacgc ttcagacggc atccggtata aagttatcct
     5941 cattatgaca ccacaagtcc tgaccatatt aggcagtacc ggcagcatag gcgaaagcac
     6001 gctggacgtt gtctcccgcc accccgaaaa attccgcgta ttcgcgctgg cagggcataa
     6061 gcaggtcgag aaattggcgg ctcaatgtca aacgttccgc cccgaatatg ccgtcgttgc
     6121 cgatgcggaa cacgccgctc ggcttgaagc cctgttgaaa cgcgacggca cggcgacgca
     6181 ggttttacac ggcgcgcagg cattggttga cgttgcgtct gccgacgaag tcagcggtgt
     6241 catgtgcgcc atcgtcgggg cagtggggct gccttccgcg ctcgcagcgg cgcaaaaagg
     6301 caaaaccatt tatctggcga acaaagagac gctggtggtt tccggcgcgt tgtttatgga
     6361 aaccgcccgt gcaaacggcg cggcagtgct gcccgtcgac agcgaacaca acgccgtttt
     6421 ccaagttttg ccgcgcgatt acgccggccg tctgaacgaa cacggcatcg cttcgattat
     6481 cctgaccgct tccggcggcc cgtttctgac cgccgattta aacacgttcg acagcattac
     6541 gcccgcccaa gcggtcaaac accccaattg gagcatgggg cgcaaaatct ccgtcgattc
     6601 cgccaccatg atgaacaaag gtttggagct gattgaagcg cattggctgt tcaactgtcc
     6661 gcctgacaag ctcgaagtcg tcatccatcc gcaatccgtg atacacagca tggtgcgcta
     6721 ccgcgacggc tccgtgttgg cacaactggg caatcccgat atgcgaacgc ccatcgccta
     6781 ttgtttgggc ttgcccgagc gcatcgattc gggtgtcggc gaccttgatt tcgacgcatt
     6841 gtccgcgctg accttccaaa agcccgactt tgaccgcttc ccctgcctga agctcgccta
     6901 tgaagccatg aacgcaggcg gagccgcgcc ctgcgtattg aacgccgcca acgaagccgc
     6961 cgtcgccgcc tttttggacg gacagattaa gtttaccgac attgccaaaa ccgtcgccca
     7021 ttgtctttca caagactttt cagacggcat aggcgacata ggggggctct tggcgcaaga
     7081 tgcccggaca cgcgcacaag cgcgggcatt tatcggcaca ctgcgctgat gccgtctgaa
     7141 caccgttatc aaaggaaaac catttgcaca cccttctagc ttttatcttc gccatcctga
     7201 ttttggtcag cctgcacgag ttcggacact acatcgttgc cagattgtgc ggcgtcaaag
     7261 tcgtacgctt ttccgtcggc ttcggcaaac cgtttttcac ccgaaagcgc ggcgacaccg
     7321 aatggtgcct cgccccgatt ccgttgggcg gttacgtcaa aatggtcgac acgcgcgaag
     7381 gcgaagtatc agaagccgat ttaccctacg cttttgacaa acaacacccc gccaaacgca
     7441 tcgccatcgt cgccgccggc ccgctgacca acctcgcact ggcggttttg ctgtacggac
     7501 tgagcttttc cttcggcgta accgaactgc gcccctacgt cggcacagtc gaacctgaca
     7561 ccattgccgc ccgcgccggc ttccaaagcg gcgacaaaat acaatccgtc aacggcacac
     7621 ccgttgcaga ttggggcagc gcgcaaaccg aaatcgtcct caacctcgaa gccggcaaag
     7681 tcgccgtcgg cgttcagacg gcatcgggcg cgcaaaccgt ccgcaccatc gatgccgcag
     7741 gcacgccgga agccggtaaa atcgcaaaaa accaaggcta catcggactg atgcccttta
     7801 aaatcacaac cgttgccggc ggcgtggaaa aaggcagccc cgccgaaaaa gcaggcctga
     7861 aaccgggcga caggctgact gccgccgacg gcaaacccat cgcctcatgg caggaatggg
     7921 caaacctgac ccgccaaagc cccggcaaaa aaatcaccct gaactacgaa cgcgccggac
     7981 aaacccatac cgccgacatc cgccccgata ctgtcgaaca gtccgaccac accctgatcg
     8041 ggcgcgtcgg cctccgtccg cagccggaca gggcgtggga cgcgcaaatc cgccgcagct
     8101 accgtccgtc tgttatccgc gcattcggca tgggctggga aaaaaccgtt tcccactcgt
     8161 ggacaaccct caaatttttc ggcaaactaa tcagcggcaa cgcctccgtc agccatattt
     8221 ccggtccgct gaccattgcc gatattgccg gacagtccgc cgaactcggc ttgcaaagtt
     8281 atttggaatt tttggcactg gtcagcatca gcctcggcgt gctgaacctg ctgcccgtcc
     8341 ccgttttgga cggcggccac ctcgtgtttt atactgccga atggatacgc ggcaaacctt
     8401 tgggcgaacg cgtccaaaac atcggtttgc gcttcgggct tgccctcatg atgctgatga
     8461 tggcggtcgc cttcttcaac gacgttaccc ggctgctcgg ttagatttta cgtttcggaa
     8521 tgccgtctga aaccgcattc cgcaccacaa ggaacttacg atgaaactga aacagattgc
     8581 ttccgcactg atgatgttgg gcatatcgcc tttggcattt gccgacttca ccatccaaga
     8641 catccgtgtc gaaggcttgc agcgtaccga gccgagcacc gtattcaact acctgcccgt
     8701 caaagtcggc gatacctaca acgacacaca cggcagtgcc atcatcaaaa gcctgtacgc
     8761 caccggtttc tttgacgacg tacgcgtcga aactgcggac gggcagctcc tgctgaccgt
     8821 tatcgaacgc cccaccatcg gctcgctcaa catcaccggc gcaaaaatgc tgcaaaacga
     8881 cgccattaag aaaaacctcg aatcgttcgg gctggcgcag tcgcaatact ttaatcaggc
     8941 gacactcaat caggcagtcg ccggcctgaa agaagaatac ctcgggcgcg gcaaactcaa
     9001 tatccaaatc acgcccaaag taaccaaact cgcccgcaac cgcgtcgaca tcgacatcac
     9061 gattgacgag ggcaaatccg ccaaaatcac cgacatcgaa tttgaaggca accaagtcta
     9121 ttccgaccgc aaactgatgc ggcagatgtc gctgaccgaa ggcggcattt ggacatggct
     9181 gacacgaagc aaccaattca acgagcagaa atttgcccaa gacatggaaa aagtaaccga
     9241 cttctaccag aacaacggct acttcgattt ccgtatcctc gataccgaca tccaaaccaa
     9301 cgaagacaaa accaagcaga ccatcaaaat caccgtccac gaaggcggac gtttccgttg
     9361 gggcaaagtc tccatcgaag gcgacaccaa cgaagtcccc aaagccgaac tggaaaaact
     9421 gctgaccatg aagcccggca aatggtacga acgccagcag atgaccgccg ttttgggtga
     9481 gattcagaac cgcatgggct cggcaggcta cgcatacagc gaaatcagcg tacagccgct
     9541 gccaaacgcc gaaaccaaaa ccgtcgattt cgtcctgcac atcgaaccgg gccggaaaat
     9601 ctacgtcaac gaaatccaca tcaccggcaa caacaaaacc cgcgacgaag tcgtgcgccg
     9661 cgaattgcgc caaatggaat ccgcgcctta cgacacctcc aagctgcaac gctccaaaga
     9721 gcgcgtcgag cttttgggct acttcgacaa cgtacagttt gatgccgtcc cgcttgccgg
     9781 cacacccgac aaagtcgatt tgaacatgag cctgaccgaa cgttccaccg gctcgctcga
     9841 cttgagcgcg ggctgggtac aggataccgg cctggtcatg tccgcaggcg tttcccaaga
     9901 caacctgttc ggtacgggca agtcggccgc cctgcgcgcc tcacgaagca aaaccacgct
     9961 caacggctcg ctgtcgttta ccgacccgta cttcacggca gacggggtca gcctgggcta
    10021 cgatgtttac ggaaaagcct tcgacccgcg caaagcatcg accagcatca aacaatataa
    10081 aaccaccacg gcaggcgcag gcatccgcat gagcgtgcct gttaccgaat acgaccgcgt
    10141 gaatttcggt ttggtggcag aacacctgac cgtcaacacc tacaacaaag cgcccaaaca
    10201 ctatgccgac tttatcaaga aatacggcaa aaccgacggc acagacggca gcttcaaagg
    10261 ctggctgtac aaaggtaccg tcggctgggg gcgcaacaaa accgacagcg cgttatggcc
    10321 gacgcgcggc tacctgacgg gcgtgaacgc cgaaatcgcc ctgcccggca gcaaactgca
    10381 atactactcc gccacccaca accaaacctg gttcttcccc ttaagcaaaa ccttcacgct
    10441 gatgctcggc ggcgaagtcg gcattgcggg cggctacggc agaaccaaag aaatcccctt
    10501 ctttgaaaac ttctacggcg gcggcctggg ttcggtgcgc ggatacgaaa gcggcacgct
    10561 cggtccgaaa gtgtatgacg aatacggcga aaaaatcagc tacggcggca acaaaaaagc
    10621 caacgtctcc gccgagctgc tcttcccgat gcctggcgcg aaagacgcgc gcaccgtccg
    10681 cctgagcctg tttgccgacg caggcagcgt gtgggacggc aaaacctacg acgacaacag
    10741 cagttccgcg accggcggca gggttcaaaa catttacggc gccggcaata cccataaatc
    10801 cacctttacc aacgaattgc gctattccgc cggcggcgcg gttacctggc tctcgccttt
    10861 aggcccgatg aaattcagct acgcctaccc gctgaagaaa aaaccggaag acgaaatcca
    10921 acgcttccaa ttccaactcg gcacgacgtt ctaatcccgc aaatgccgtc tgaagccctt
    10981 cagacggcat ttcgcggcaa cattcgaagg agttttacca tgacccgttt gacccgcgcg
    11041 tttgccgcgg ctctgatcgg tttgtgctgc accgcaggcg cgcacgccga caccttccaa
    11101 aaaatcggct ttatcaacac cgagcgcatc tacctcgaat ccaagcaggc gcgcaagatt
    11161 caaaaaacgc tggacagcga attttccgcc cgtcaggacg aattgcaaaa actgcaacgc
    11221 gaaggtctgg atttggaaag gcagcttgcc gaaggcaaac tcagaaacgc aaaaaaggcg
    11281 caagccgaag aaaaatggcg cgggctggtc gcagcgttcc gcaaaaaaca ggcgcagttt
    11341 gaagaagact acaacctccg ccgcaacgaa gagtttgcct ccctccagca aaacgccaac
    11401 cgcgtcatcg tcaaaatcgc caaacaggaa ggttacgatg tcattttgca gaacgtgatt
    11461 tacgtcaaca cccaatacga cgttaccgac agcgtcatta aagaaatgaa cgcccgctga
    11521 ccctttcaga cggcataccg aacaggaaaa ccatgattcc ggccacctac accctgtccc
    11581 aaatcaccgc gcggctcggc ggcgaatggc gcggcgagga catttccgtt accgccgtgc
    11641 gcccgctcgc agacgcgcag gcggaacaca tcagcttcct tgccaatccg aaatacaaag
    11701 ccgaagtcca cgacagcagc gcgggcgcgg tcatcgtttc cgccaaagcg gcagacggat
    11761 ttgaagggcg caacctgatt gtcgccgacg acccctatct ctatttcgcc aaagtcgccc
    11821 gcctgttttc acccgtcgtc aaagcgcgcg gcggcatcca tccgaccgcc gtcgtcgaac
    11881 cgagtgccac cgttcccgca agctgcgaaa tcggcgcgaa cgcctacatc ggcgcaaaca
    11941 ccgtgctcgg cgaaggctgc cgcatcttgg caaacgccgt cgtccaacac gattgcaaac
    12001 tgggcgacga agtcgtcctg catcccaacg ccgtcgttta ttacggctgc acactgggca
    12061 gacgcgtcga aatccacagc ggcgcggtca tcggcgcgga cggtttcgga ctcgcttttg
    12121 ccggcgattc atggtttaaa atcccgcaaa ccggcgcggt aacgctgggc gacgacgtag
    12181 aaatcggctc gaacaccaac atcgaccgcg gcgcgatgag cgacaccacc gtcggcaacg
    12241 gcaccaaaat cgacaaccaa gtccaaatcg gacacaactg caaaatcggt tcgcacaccg
    12301 tcatcgccgc caaaaccggc atctcaggca gcgtaaccat aggcagctac tgcatcatcg
    12361 gcggcggcgt cggtacggtc ggacacatcg aaatcgccga caaaaccacc atcggcggcg
    12421 gcacgtccgt cacccacagc attaccgaaa gcggcaaaca cctcgccggc atcttcccga
    12481 tgtccaccca taaagaatgg gcgcgcaacg ctgtttacat ccaccgctta agcgaaatga
    12541 acaaacggct caaaacactg gagcagcagc tttcagatgc cggtcaagac agcaaataac
    12601 caaaccgact ttattcaagg aatacgacag acatggacgt acaactcccc atcgaagcca
    12661 aagacatcca aaaactcatc ccccaccgct atccgtttct ccagctcgac cgcatcaccg
    12721 ccttcgagcc gatgaaaacc ctgaccgcga ttaaaaacgt cagcataaac gagccccagt
    12781 tccaaggcca ctttcccgac ctgcccgtga tgcccggcgt actcatcatc gaagcgatgg
    12841 cgcaagcctg cggcacgttg gcgattttga gcgaaggcgg gcgcaaagaa aacgaattct
    12901 tcttcttcgc cggcatagac gaagcccgtt tcaaacgcca agtcatcccc ggcgaccaac
    12961 tcgtctttga agtcgagctg ctgaccagcc ggcgcggcat cggcaaattc aacgccgttg
    13021 ccaaagtgga cgggcaagtc gccgtcgaag ccatcatcat gtgtgccaaa cgcgtggttt
    13081 gagcgttcgg aaaaaggtcg tctgaaagtt tcagacgacc taataaaaag aaaacccacc
    13141 ggtttaggtt tattcaaacc cagggacacg gtggggatta gttttaccta actgtatcaa
    13201 atatagtttg aaacgtcaat ttaaaaataa agaatcaggt gttagcgtgg attataagga
    13261 atgctgtagt attttttctg atttcagaat ggaacggtat aaaaatgccg ttggggaaga
    13321 caaagcagct gaattgtatt tattgaattt atctt
//
LOCUS       DAWEWP010000044        13346 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWEWP010000044 DAWEWP010000000
VERSION     DAWEWP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.044"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(24..359)
                     /locus_tag="WGN53_000918"
     CDS             complement(24..359)
                     /locus_tag="WGN53_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ0139928.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(439..2568)
                     /gene="dnaX"
                     /locus_tag="WGN53_000919"
     CDS             complement(439..2568)
                     /gene="dnaX"
                     /locus_tag="WGN53_000919"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ0139929.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLHNMTSQQVADHLAHVLDSEKIAYEPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDTNAVIENTELKSPSAQTAEKETAVK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAGPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNVTDAPLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDGAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1340..1379)
                     /gene="dnaX"
                     /locus_tag="WGN53_000919"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2663..4639)
                     /gene="mutL"
                     /locus_tag="WGN53_000920"
     CDS             complement(2663..4639)
                     /gene="mutL"
                     /locus_tag="WGN53_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ0139930.1"
                     /translation="MSRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSDNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENNSENLFDRASNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSPSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQRLLIPVTFAASHEECAALADHAEALAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLNEFAQVGSSQTIEEHENHILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4783..4968
                     /locus_tag="WGN53_000921"
     CDS             4783..4968
                     /locus_tag="WGN53_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ0139931.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            4979..5647
                     /locus_tag="WGN53_000922"
     CDS             4979..5647
                     /locus_tag="WGN53_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ0139932.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLMRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVSFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIVQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            5647..6363
                     /locus_tag="WGN53_000923"
     CDS             5647..6363
                     /locus_tag="WGN53_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ0139933.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRVRMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            6412..6897
                     /gene="purE"
                     /locus_tag="WGN53_000924"
     CDS             6412..6897
                     /gene="purE"
                     /locus_tag="WGN53_000924"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ0139934.1"
                     /translation="MTQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7080..8534)
                     /locus_tag="WGN53_000925"
     CDS             complement(7080..8534)
                     /locus_tag="WGN53_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ0139935.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPILGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8531..9232)
                     /locus_tag="WGN53_000926"
     CDS             complement(8531..9232)
                     /locus_tag="WGN53_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ0139936.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9229..10008)
                     /locus_tag="WGN53_000927"
     CDS             complement(9229..10008)
                     /locus_tag="WGN53_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ0139937.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            10292..11698
                     /locus_tag="WGN53_000928"
     CDS             10292..11698
                     /locus_tag="WGN53_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0139938.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLNMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            11810..13336
                     /locus_tag="WGN53_000929"
     CDS             11810..13336
                     /locus_tag="WGN53_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0139939.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPKYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
BASE COUNT         2708 a         3606 c         3759 g         3273 t
ORIGIN      
        1 tgcgtgggaa tgacggtcgg gtatcagcgg aagaagtcgc ccactccggg gggtagacct
       61 tgcgtgaatg cgcccattgt tttgtttgcg gtttcttcgg ctttgccccg ggcggatttg
      121 agggcggcga ggatgaggtc ttcgagcatt tctttgtcgt cggcggcttc ttgaatcaaa
      181 tcggggctga tgtcgatttt gcgtacttcg tgcgcgcagg tcattgtgat tttgaccagg
      241 ccgttgcctg cttcgccttc gatttcggtt tcggcaagtt tggcttgcgc ttttttcata
      301 ttttcctgca tttgctgcgc ctgtttcatc aggccgccta atccggcttt tccgaacata
      361 ctgaatactc ctgtctgttt tgattgaatg agggaaacgg caatgccgtc cgaagggttc
      421 gggcggcatt atatctgttt atggccggtt tgctgccaat tccagtgatt cgggctgcca
      481 ttgcgcgccg aatgcttgga ggattttttg tgcggcgggg tcggcttcga gcaatgcttg
      541 tgctttttgc ctgtcttcgg cttggacgcg cttgtcctgc atcgcggggg tttccctgcc
      601 ggcttcgtcg cgccagtctt gggtttgcag ggtgagttgc agtccgtagg cttgggcaag
      661 ggtgtcgcgg attttgtcga gacgtttttt gtcggcggtg gcgcgggctt cggcggtcat
      721 cgccaaaacc atcaggccgg tgtcgggatg gtattccgtc cacgcggagt gttgcgccgg
      781 catttgcgcc gcgccgagtt tgcgggcgaa atgccggacg atggctgccc agttttcggt
      841 ggaaaattcg ggcggcgggg cggacggcgt gttgtttccg ccgatgccgc ctgcttctgc
      901 ttcttcgtcc gcgcctccgc ctgcggtatc ggcaggggcg gcgtgttccc aatcgggcgg
      961 cgggatttct gcgccgtctt ctacggggca gtcattatcc ggaaagccgt aaccgtagaa
     1021 aggttctgca ggggcttcgt gtgcaaatgt ttccgtttca acggcttcat cattcggtgt
     1081 tgcctgaatg gggttttcag acggcacttc ggacaagggg gcatcggtta cgttgtcggc
     1141 tgccttgttc ttggaaactt ggttttcggg cggcgtttcg gcttcggatg ccgtctgaat
     1201 gctttttgcc gacgtttgcg ccgtgccggc tgcggcttgg gcttcgtctg gcgcgtcttc
     1261 ccaaggcgga acatcgttgt tttcttgatt ggaaacgggt ccggcagttt tgccttcaga
     1321 cggcattgcc gctgcggatg ccgtctgaac gggtgtttgg gcggtttctg cttcagggcg
     1381 cggttggggc ttttttacgg cggtttcctt ttcggcggtt tgtgccgatg gggattttag
     1441 ttcggtattt tcaatcacgg cgtttgtatc acacgaagct gccgccaagg gcgcaaacgc
     1501 cagcatacgc agcagggtca tcataaagcc ggcgtattcg tcgggggcga ggctcaggtc
     1561 gcgtttgccg tggacggcga tttggtagta aagctggatt tgttcgccgc ttatggtttg
     1621 ggcgaggcgg tgcaaaatat cggaatcggg gtcgtcgtgc gccaaggcgg agggcaccgc
     1681 ctgtatcagg gcgaggtgtt gcagcagtat ggcaagttcg cccaaggcgt tgtcaaagcc
     1741 gacggcacac gccgccattt cctgcgcttt ggcggtcagg gctgcgccgt cttggttgat
     1801 gatgcctgtc aagagttcgt aaaggtattg tttgtcaacc gcaccgatca tttggcggac
     1861 gtcttgttcg gcaacgctgc ccgaacccat ggcgatggct tggtcgagca ggctcaaggc
     1921 atcgcgcatc gatccggcgg cggcgcgtcc caaaagttgc agggcagggg gttcgtaggc
     1981 gattttttcg ctgtcgagga cgtgggcgag gtggtcggca acctgctgcg aagtcatatt
     2041 gtgcaagaca aattgcaggc agcggctcaa gacggtaacg gggactttat gcggatcggt
     2101 cgtggcaagg atgaatttga cgtgttcggg cggctcttcc agcgttttga gcatagcgtt
     2161 gaacgcgctt ttggaaagca tatgcacttc gtcgatgata tagactttgt atttgccggc
     2221 agtcggtgca tattgggcgt tttccaatac ttcgcggatg ttgtcgatgc ctgtgttgga
     2281 ggcggcgtcg atttcgagca ggtcgacata gcgtccggca tcgatttggg tgcagctttc
     2341 gcacacgccg caaggttcgc cgtgttgcgc gttttcgcag ttgaggcttt tggcaaggat
     2401 gcgggcgatg gtggttttgc ctacgccgcg cgtaccggtc agcaggtagg cgtggtgcag
     2461 cctgccttcg tccaaggcgt tttgcaaggc tttgacgacg tgttcctgac cgactaagtc
     2521 ggaaaaggtt ttgggccgcc attttcgggc gagaacttga taggccatgt ttttctcttg
     2581 gtttcggtcg tgatgtttct gtcggtgcgt cggaatgccg tctgaacggc ggcttcgggc
     2641 ggcgtattct agcactttcg gtttattgtc cgcgcaagaa cagtgcgtcc aattctttca
     2701 aagtcagttt gacccaagtc ggcctgccgt ggttgcactg gttgctgcgc ggcgtatttt
     2761 ccatatcgcg cagaagggcg ttcatttcgg gcagggtgag ccggcggccg gcgcggatcg
     2821 agccgtggca ggacatggtg gcgaggatgt ggttttcgtg ttcctcgatg gtttggctgc
     2881 tgccgacttg ggcgaattcg tttaatacgt cgcgggcgag ggagacgaca tcggatttgc
     2941 ccaacattgc gggtgcggcg cggacggcga gggtgttgcc gcccatatcg gacagttcca
     3001 gcccgaagcc tgccagtgct tcggcgtggt cggcaagggc ggcgcattct tcgtgggacg
     3061 cggcaaaggt tacgggaata agcaggcgtt ggctttgcag gttgccgttt tcctgacgtt
     3121 ggcgtttcat tttttcgtag ttgacgcgtt cggcggcggc gtgcatatcg atgagcaaca
     3181 ggctgtcttc ggcttgggca agaatgtaga tgccaagtaa ttgggcaatg gcgaaaccga
     3241 gcggcggcag ttcggatggg gacgggatgc cgtctgaaag cggcgtatct gtttggggag
     3301 caggcgtttc agacggcata ttgccgaaac gtgcctgctc gaatcggctt aactcaaggt
     3361 cgatgtcgtc ggtttttttg taaagttcgg cgtaagtatt cattgccgcg cggctttcgc
     3421 gcagggacag gctgcgttgt tgcggcgcat atgcggactg atagggcatg ggcgcggttt
     3481 tgcctgatga accaaaggca ttgtgtgtat ctgatttgtt gcctgtcggg tagttggatg
     3541 cgcgatcaaa cagattttcg ctgttgtttt cagacggcat tggtgcaggc gtaacgccgg
     3601 taatgtcatg caacacttcg cctgcgttgc tgacgctttc ggtcaggtcg gcgcgtgtgt
     3661 cggcaagggc tttgttgagc gtgtggaaca caagttggtg cacctgctga ctgtcgcgga
     3721 agcggatttc ggttttggtc gggtggacgt tgacatccac ggcttcgggc ggcaggtcga
     3781 gaaagaggac gaaagcaggc gtcagtgcgt tgtgcaatac gtcgcggtat gcctgcttga
     3841 cggcatggag catgactttg tcgcggacaa aacgatggtt gacgaagcag tattgtttgt
     3901 cggttttacc tttggcgaaa gtcggtttgg caatcgcgcc atagagccgc aatgcgccgt
     3961 tgtcgctgtc aatttccaat gatgccgtct gaaaatcatc gccgacaatg gcggcaatcc
     4021 gttcatgcag gctttgtgca gggagtttga acacttgttt gccgtcgcgt ttgagcgaga
     4081 aggcaatgtg cggatgcgcc agcgcgaggc gttcgagcat agtggcgcag tgggcgtatt
     4141 cggtggcttc agacttgagg aacttgcgcc gcgcaggggt attgaagaag agttcggcgg
     4201 cttcgatggt ggtgccgacg gggtgggcgg cggcggtggg gctgctgagt ttgccgtctt
     4261 cggctttgac ttgggttgcg tgcgaactgt cttcttgacg gctggtcagg gtcaggcggc
     4321 tgacggaggc gatgcttgcc aagccttcgc cgcgaaagcc catgctggca acgtgttcca
     4381 aatcgtttaa ggttttgatt ttgctggtag cgtgtcggtg gagcgcaagc tcgatgtcgt
     4441 cggggtggat accgccgccg ttgtcgctga cgcgaatcag gcggatgccg ccgcccgcca
     4501 gctcgacatc aatcgccgtt gcgcctgcat cgatactgtt ttcaacaatt tctttcaagg
     4561 cgttggcagg gcgttcgact acttcgccgg cagcgatttg gttgacaaga tggtcgggca
     4621 gggcggcaat tcgggacatg gcatgggctt ccgtttcaaa aaacgcttta ttctataata
     4681 aaacccctta tctttctgcc cgtattcttg ataaaagccg tttatcccgt ttggaaaata
     4741 ccagtataat cacgccgtat cccgtaaaat tggagcgcaa agatgtacca ctaccaatcc
     4801 gaagccacac aattcctcaa ccgcctgatt gaagaaaaac ctgagttgga acagcagcgt
     4861 ttggaaaacc gggggctttt gtgggatgtc gaactcaatc ccgaagaaca ggaaaacttc
     4921 gaggcggcaa aagtagcgaa aaaaccttat acctattacc aagactgaac ccgccgaaat
     4981 gacgacccat ctgtccaatg tcgcccccga cctgcaaaac tatttgaacg ccatcggcga
     5041 acctgaacat cccgttttga tgcggctgcg cgagaagacc gggcatcacc gtatgggcaa
     5101 aatggcgatt gcgcgcgaac aggcggcggt tttggtttgg ctggcaaagc tgatccgtgc
     5161 ggaaaaatat ctggaagtcg gcgtgtttac tggatacagc agcaccgcgc ttgcattggc
     5221 gttgcccgaa cacgggcgga ttaccgcctg cgacatcaat gtaagcttta ccgatacggc
     5281 gcgtcaggtt tggaacgagg caggtgtggc acataaaatc agcctgcacc tgcaacccgc
     5341 attgctgaca ttggatgatt tgattgtaca gggtgaagcc ggaagctacg atttggcact
     5401 gatagacgca gacaaaccgc ccacgccgca atatttcgag cgttgcctca aactcgtccg
     5461 gcaaggcggc atcattgcca ttgacaatat tttgttgaac ggaagggtga tgcgcgaagc
     5521 ggcttccgac gcgccgccca gtgtcggcat cctcaaagat ttcaatcaaa acctgccgaa
     5581 cgacccgcgc atcgtcccca tcaccctgcc cgtcggcgac ggtttgaccc tgcttctgaa
     5641 aaaataatga agaccaaatt accgcttttt atcatttggc tgtccgtatc cgccgcctgt
     5701 tcttcccctg tttcccgcaa tattcaggat atgcggctcg aaccgcaggc agaggcaggt
     5761 agttcggacg ctattcccta tcccgttccc actctgcaag accgtttgga ttatctggaa
     5821 ggcacactcg tccgcctgtc gaacgaagtg gaaaccttaa acggcaaagt caaagcactg
     5881 gagcatgcga aaacacaccc ttccagcagg gcatacgtcc aaaaactcga cgaccgcaag
     5941 ttgaaagagc attacctcaa taccgaaggc ggcagcgcat ccgcacatac cgtcgaaacc
     6001 gcacaaaacc tctacaatca ggcactcaaa cactataaaa gcggcaggtt ttctgccgct
     6061 gcctccctgt tgaaaggcgc ggacggaggc gacggcggca gcatcgcgca acgcagtatg
     6121 tacctgttgc tgcaaagcag ggtgcgtatg ggcaactgcg aatccgtcat cgaaatcgga
     6181 gggcgttacg ccaaccgttt caaagacagc ccaaccgcgc ccgaagccat gttcaaaatc
     6241 ggcgaatgcc aatacaggtt gcagcagaaa gacattgcaa gggcaacttg gcgcagcctg
     6301 atacaggctt acccgagcag cccggcggca aaacgcgccg ccgcagccgt acgcaaacga
     6361 tagtttttcc atttttccgc tttttacccc atccgcacca aggagagatt tatgacacaa
     6421 atcggcatca tcatgggcag caacagcgat tggcccgtta tgcggcaggc agcgcagttt
     6481 cttgaagagt tcggcgtaga atatgaggcg cgcgtcgttt ccgcacaccg cactccggat
     6541 ttaatgttcc aatacgccga aaccgcacgg gcgcgcggca tcaaagccat catcgcgggt
     6601 gcgggcggcg cggcgcattt acccggcatg gttgccgcca agaccaccgt ccccgttttg
     6661 ggcgtacccg tccccagcaa atacctgcgc ggcgaagatt cgcttttatc gattgtacaa
     6721 atgcccaaag gcgtacccgt cgccacattc gccatcggcg aggcaggcgc ggcaaatgcc
     6781 gcattgttcg ccgtttccat gctggccaac gaaaaccccg aactggcgca aaaactggca
     6841 gacttccgag ccaaacagga acaggctgtt ttgagtatgg aattggaaca gatttaaaac
     6901 ccgccgtccg cgaatatata gtggcttaaa tttaaaccag tacggcgtta cctcgccttg
     6961 ccgtactatt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta
     7021 tacaaatgcc gtctgaagcc ctgtttcggg tttcagacgg catttttgca aacaactgtt
     7081 tatgcctgtt ctttttggcg cattttttct gccattagtt cgtgcagggt tttcttcgcc
     7141 ggtttcatcg gcacgcggtt ttgcgtccaa cccaactgtt tgcgcggggt caggttgcgg
     7201 aacttggtgg ctgcccaacc gaaggcgcgg taggttttgc tgccgctgaa aataccgttg
     7261 aatgtgcgcc acgccatttg ttcgccgaag gtatgcgatg cgccttgccc taggatgggg
     7321 tgtggcacgg tttcggtcgg cgaacgttgc gcttcaacgc gcaaacgctg catttgttcg
     7381 gtaatcggga tgcgtaccgg acagacttcc acacacgcgc cgcacatggt acaggcggtt
     7441 ggcaagtcgc gggtggcatc cagacccaac aggtgagggg aaataatctc gccaatcggg
     7501 ccgggatagg ttgtgccgta tgccgcgccg ccgatacggg tataaaccgg gcaatggttc
     7561 atacacgcgc cgcaacggat acattgcaag gtgcggcgca tttggtcttc ggcataggct
     7621 tggctgcgac cgttgtcaag caggaccaag tgcatttctt gaggaccgtc cagctcttcg
     7681 ctacggcgcg ggccggtaat catattgaaa taagtggtaa tgttctgacc aatggcagaa
     7741 cgcggcagca ggctgtacaa gggtgggatg tcggacaatt tcgccaccac tttttcaatg
     7801 ccggtaatag cgatatgcac gggcggtacg gtggtactca agcgaccgtt gccttcgttt
     7861 tccaccagac acagcgtacc cgtttcggca acagcaaagt ttacgccact caaaccgaca
     7921 tcggcagtgc tgtaaatatc gcgcagggct ttgcgggcga agccggtcag ttggtccacg
     7981 tcgtccgtaa gcggagttcc aaggttttgg tggaacagtt cactcacctg ttctttggtt
     8041 ttgtgaatgg cgggcatcac gatatgggtc ggtttttcgc ctgccatttg gacgatgaac
     8101 tcgcccaagt cgctttctac cgctttaatg ccttttgctt caagataatg gttcagctcg
     8161 atttcctcgc tgaccatcga tttgcctttg accatcagct tgccgttttt ggctgtgatg
     8221 atgtcgtgga taatttggca ggcttcggtc ggggtttctg cccagtgcac tttcactccc
     8281 aacttagtca ggttttcttc cagctgctcc agcagggctg gcaatttaga caatgagcgc
     8341 tgacggacgt gttcgcacaa atcgcgcagg ctttgcagct cttcttcgtc ggtcaaaacg
     8401 gctttgcgtt tggtcatcag catatccatc gcggtacgca ggcttttgcg caaaggcttg
     8461 tcttgaaggg aaattgcggc gttttgcttg aaagtttccg gcttcatgtg aaatttgatg
     8521 gtttgcgtag tcatgcgttt tcctccaaat cggcagggga aatgtggtcg ggcaggatgg
     8581 cgaggatgac caaatcgcgc gggccgtgcg cgccgtaagc aagcgtcagt tggatgtctg
     8641 cggttttgga cgggccggaa atcaggaata cattggtcgg cataccgttt tccaccagtt
     8701 tttcgccttc gacggcatta tgaaactcgt tgtacatctt ggacgtatcg aacaggcaga
     8761 aatgcacggg cggaacgaga ctcaaagtac gcggttcttc ggggctggaa aacagcatca
     8821 gcgtgccggt gcgggcgatg ccgcattgcg cgccgctgaa gcccgcatcg atgttcgtga
     8881 aaaactcggt tttccaagta tcgatttcgc gctcgaaggc aatcggttcg atattgctgt
     8941 ccgccaatgc ggcacgggca atttgtccgt gttcggtcgc caagggcagc aggatgtttt
     9001 tcaaaccctt gccttctgcc gcttcgcgga aaacctgcat ccaattgctt ttcgtcaccc
     9061 aataaatttc ggttttgacc gcacgcatag cggcagccca atgtttcaga cgctcaactt
     9121 cgctgcccca agaaacaccc atttcacggt aataatcaaa aaccgcaggt tcttccatcg
     9181 gcaatgcgtc ggcttttttc agttttgcca aaatattttc gcgcgcgctc atgctttgcc
     9241 tccggtacgt tccaacaaga aggatgcgat atgtttcgga cgcggcatat tcggttcgtc
     9301 cttagcaatt ttgccgccga tgttcatcat acagccgcag tccgcgctga tgatttcggt
     9361 tgcgccggtt tctttcagcg cggcgacttt gtcggttacc atcgcgccgg aaatatcggc
     9421 ttgtttgacg gagaatgtgc cgccgaagcc gcaacattcg ctttcgtggt cgtggacgat
     9481 gcgttcgacg ttttccatac cgtcaatcag ttgccagccc gagagatgga cattcatttc
     9541 gcggcgggcg gcgcaggaag tgtgaacggc gactttgagc ggttcgccct tgtcttcggg
     9601 tttgaaaccg atggcaagca ggaaatgggt aaactcgatg atgcggccgg cgcaatccac
     9661 agccctttcc tcgtactcgc tgcctttaaa cagcgtcggc caatggtgct tcatcatacc
     9721 gccgcacgaa ccggacggta cgacaatcgg ccactcctcg gggaaaaggt cgagttgcgc
     9781 tctggcgaca tcaaaggctt cggtcggatg gccggatgaa taggcgggct gaccgcagca
     9841 gctttgcgcc atcgggaaat ggacgcgtat gccctgttgc tcgattaggg taatggcatc
     9901 catgcctgct tcgggcataa aaagatcaag gacgcaagtg ccgaaaaaat agacatcggt
     9961 cggattgctg tcgtatcgga tgatttttgg gggaatggtt gcgctcatgt tttttgataa
    10021 cgggaaaccc gtttttttct gtagaaaggt aagcgtttac tttaagtaat tgactgttgc
    10081 gggtcaagtc taattttaaa aaataatccg gtttttctta caaactaccc cataacgctt
    10141 actgtacctt aatctgatag ttttcgataa taattatcat tacaatgcaa tgccggttcg
    10201 tttgcttgtg aacattcaag atgccgactc tgacggcatt cagacagcat ctgaaaacaa
    10261 taacggcaca aggatggggt acaatcgctc tatggaaaaa aacaccctat ccgcccgcgc
    10321 accatcccct tggctgccgc ttctgctggc aattgccatt tttatgcaga tgttggatgc
    10381 gaccatttta aataccgcac tgcctgaaat tgccgccgac ctgaatgagt cgccattgaa
    10441 catgcagctg gcggttattt cctacacgct gacggttgcc ctgctgattc ctttgagcgg
    10501 ttatttggcg gacaggttcg gaacgaaaaa agtctttttc ggttcgattg ccgtttttat
    10561 gctcggatcg gcattgtgcg ccgcatcggg ttcgctgttt gaattgacgc tttcccgtgt
    10621 cgttcagggc atcggcggtt cgatgctggt tccgataccg cgtctgacca tcttgcgtgt
    10681 gtacgacaag tccaagctgc tcaacgccat caattatgcg gttatgcccg cattaatcgg
    10741 accggtttta gggcctttgg cgggcggtta tctggtcgaa tacgcttcgt ggcactggat
    10801 tttcctgctc aacctgccca tcggtcttct gggtttcata ttgggacgca acatcatgcc
    10861 cgatattaaa ggcagcaata tctctttaga cttcaaaggc tatctgattt tttctgccgc
    10921 cgcgtgcctc ttgttacttt cggcagaaag cctgtcgcac gcgctgcctc cgtattttgc
    10981 actgttgccg ctgtgcggcg gactgctgtt tgcacgccgt tatttccgac atatgaaaac
    11041 cgcgtccaaa ccgatttatt ccgccgacct gtttctgata cgcactttcc gtctaggact
    11101 ggcgggcaat ctgttcagcc gtctcggcat cagctcgatt ccttttctga tgcccctgat
    11161 gtttcaagtc gctttcggct tcggcgcaag cctgtcgggt tggctggtcg cccccgtcgc
    11221 cctgtcttcg ctgctggtca aaccgctgat tgcaccgctc atgaaacgtt tcggctaccg
    11281 cacggtactg ctttggaaca ccaagctgct tgccgccttc atcatgctgc tcgccctacc
    11341 tgatggaaac tcgccgctgt ggatttggat tttcctctcg ctggcgatcg gcgcgtgcaa
    11401 ctccctacag ttttctgcca tgaacacact gaccctcgcc gatttgcgcc cgcaacagac
    11461 cggcagcggc aacagcctga tggcggtcaa ccaacagctt gccatcagca tgggcattgt
    11521 tgtcggcgca ttaatcctta aaaactggac atttctgata ccggcttctt caggtctgca
    11581 ttccgccttc cgtatgaccc tgctcagtat cggcggcatt acccttgcct cgtcgttggt
    11641 tttcaagcgt ctgcacgttt cagacggtgc aaacctgaca cggaacacac cgtcctaaag
    11701 cggtccgcac gcaaaacttt tacccgtttc aacgtttgga ttatgattcc gcacttccat
    11761 gcgcaccaac cccaaaacac aggcaatgcc gtctgaaacc atatccctga tgaaaacacg
    11821 cagcctaatt tcccttttat gcctccttct ctgttcatgt tcttcatggt tgcccccact
    11881 ggaagaacgg acggaaagcc gtcatttcaa tacttccaaa cccgtccgcc tggacaacat
    11941 cctgcaaatc cggcacaccc ctcataccaa cgggctatcc gatatctatc tgttgaacga
    12001 cccccacgaa gcctttgccg cccgcgccgc ccttatcgaa tctgccgaac acagcctcga
    12061 tttgcaatac tacatctggc gcaacgacat ttccggccga ctgctgttca acctcgtgta
    12121 ccttgccgca gaacgcggtg tgcgcgtacg cctgctgttg gacgacaaca acacgcgcgg
    12181 attggacgac ctcctgctcg ccctcgacag ccatcccaat atcgaagtgc gcctgttcaa
    12241 ccccttcgtc ttacgaaaat ggcgcgcact cggctacctg accgacttcc cccgcctcaa
    12301 ccgccgcatg cacaacaaat cctttaccgc cgacaaccgc gccaccatac tcggcggacg
    12361 caatatcggc gacgaatact tcaaagtcgg tgaggacacc gttttcgccg acctggacat
    12421 cctcgccacc ggcagcgtcg tcggcgaagt atcgcacgac ttcgaccgct actgggcaag
    12481 ccattccgcc cacaacgcca cgcgcatcat ccgcagcggc aacatcggca agggtcttca
    12541 agcactcgga tacaacgacg aaacgtccag acacgcgctc ctgcgctacc gcgaaaccgt
    12601 cgaacagtcg cccctctacc aaaaaataca gacaggacgc atcgactggc agagcgtcca
    12661 aacccgcctc atcagcgacg accctgcaaa aggactcgac cgcgaccgcc gcaaaccgcc
    12721 gattgccggg cggctgcaag acgcgctcaa acagcccgaa aaaagcgtct atctggtttc
    12781 accctatttc gtccccacaa aatccggcac agacgcactg gcaaaactgg tgcaggacgg
    12841 catagacgtt accgtcctga ccaactcgct acaggcgacc gacgttgccg ccgtccattc
    12901 cggctatgtc aaataccgaa aaccgctgct caaagccggc atcaaactct acgagctgca
    12961 acccaaccat gccgtccctg ccacaaaaga caaaggcctg accggcagct ccgtaaccag
    13021 cctgcatgcc aaaaccttca ttgtggacgg caaacgcatc ttcatcggct cattcaacct
    13081 cgacccccgt tccgcacggc tcaatactga aatgggcgtt gttatcgaaa gccccaaaat
    13141 cgcagaacag atggagcgca cccttgccga taccacaccc aaatacgcct accgcgttac
    13201 cctcgacagg cacaaccgcc tgcaatggca cgatcccgcc acccgaaaaa cctacccgaa
    13261 cgaacccgaa gccaaacttt ggaaacgcat cgccgcaaaa atcctatccc tgctgcccat
    13321 agaaggttta ttatagaaat gtagcg
//
LOCUS       DAWEWP010000045        13239 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWEWP010000045 DAWEWP010000000
VERSION     DAWEWP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.045"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(44..811)
                     /locus_tag="WGN53_000930"
     CDS             complement(44..811)
                     /locus_tag="WGN53_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0139940.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGALTALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVYETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(899..1726)
                     /gene="mutM"
                     /locus_tag="WGN53_000931"
     CDS             complement(899..1726)
                     /gene="mutM"
                     /locus_tag="WGN53_000931"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ0139941.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1778..2443)
                     /locus_tag="WGN53_000932"
     CDS             complement(1778..2443)
                     /locus_tag="WGN53_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEZ0139942.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     IAD"
     gene            2622..4598
                     /locus_tag="WGN53_000933"
     CDS             2622..4598
                     /locus_tag="WGN53_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0139943.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4666..5358
                     /locus_tag="WGN53_000934"
     CDS             4666..5358
                     /locus_tag="WGN53_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ0139944.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAVGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKF"
     gene            5901..6344
                     /locus_tag="WGN53_000935"
     CDS             5901..6344
                     /locus_tag="WGN53_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0139945.1"
                     /translation="MTVAGATVGFGATWRFPYLVGENGGGAYVFLFCIAMLVIGIPMI
                     LVENVIGRRKGVNALDAFGGPMNGKPIAKIWKLVGWMGLLGAFGIMAYYMALGGWVIS
                     YIVNIIGGNLNISSPIDGVVTKGFFAEHIETALGKLRFIRCFLSP"
     gene            6404..7213
                     /locus_tag="WGN53_000936"
     CDS             6404..7213
                     /locus_tag="WGN53_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0139946.1"
                     /translation="MPLLFLFLIAMVVRNVTLPGAMEGVAFYLKPDFSKITAELFVFV
                     LGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAILAGFMIFPSLFSFG
                     VAPDSGPTLVFQSLPIVFSHMWAGSVFAVIFFSLLLIAALTTSLTIYEVLITTIQEKT
                     KIRRTAAITIVLAAIFIFGNIPSILSYGPWKDVSIFGKNIFDAFDYISGNILFMLTAL
                     GSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLIFISNLF"
     gene            7457..8113
                     /locus_tag="WGN53_000937"
     CDS             7457..8113
                     /locus_tag="WGN53_000937"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ0139947.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            8269..9954
                     /gene="rpsA"
                     /locus_tag="WGN53_000938"
     CDS             8269..9954
                     /gene="rpsA"
                     /locus_tag="WGN53_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ0139948.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGDILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            9965..10279
                     /locus_tag="WGN53_000939"
     CDS             9965..10279
                     /locus_tag="WGN53_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ0139949.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(10629..11036)
                     /locus_tag="WGN53_000940"
     CDS             complement(10629..11036)
                     /locus_tag="WGN53_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ0139950.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            11184..12293
                     /locus_tag="WGN53_000941"
     CDS             11184..12293
                     /locus_tag="WGN53_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ0139951.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            12302..13129
                     /gene="fghA"
                     /locus_tag="WGN53_000942"
     CDS             12302..13129
                     /gene="fghA"
                     /locus_tag="WGN53_000942"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ0139952.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDSSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         3509 a         3633 c         3047 g         3050 t
ORIGIN      
        1 atccgcaaaa gcgaatcata ctgccacatt ccggcaaccg gtttcagaca gcgatatccg
       61 catcgtcggc gacaacggca cggatgcttt cttcgatttt atcttttaaa gcataacggt
      121 cttcgctttc cgccgcatcc gccacgcaaa cgaaatcgac ttttatcgtt aattttttca
      181 tagacacaat gcgccacagg caggtcggca aaccgacatc ggcatatgag ggacgagccg
      241 tcctttttcc cgtttcgtca taataacgca gcgcgaccgc caaaaccttt gcccccgcat
      301 caatggcgga ttggaacagc gcggctttga acggcaggag ccccagtccg gaggaagtcc
      361 gcgcttcggg gaaaaaactg acgttttgac cgcgttgcaa ggtttcgtag acggcgcggt
      421 taatcggttc gatgtcgcgc cgcgaattgc ggttgatgaa caccgttccc gcgttctgcc
      481 ccatcttgcc caataccggc cagcttttga tttcctgctt ggcgataaag ctgctcggat
      541 aaaccgcgct catcgcgaaa atatccagcc aggacacgtg gttggcggca accaagacac
      601 cgttcggatg ttcgggtgcg ggcctgccca cctccaatcc gatatccaaa gccgtcagag
      661 cccccctgcc caactcgatt accgcccgat tgcgcgactc ggggcagccg ccgtcaatac
      721 cgcgcaggtt tttgccggtt ttgaatagcc agaccgtcaa gcggcacaag cggcgcagac
      781 gtgtaaaaaa tgaagcttta tttgaagaca tgaacaattc tttcccgata atcggtgttt
      841 gttgttgaaa cgttcggacg gcataccgat aaaatgccgt ctgaaaccgt tttccgccct
      901 atttctgaca attcgggcag taaaacgtgc cgcgctgccc caaagtttct ttcacaacca
      961 aaccgccgca ctgggggcac ggctgattgt gccgcccgta caccgtatat tcctgttgga
     1021 agtagccact tttgccgtcg ctgtccacaa aatccctcaa ggtactgccg cccgtttcaa
     1081 tggcgcgccg caacaccgct ttgacggttt caaccaaaag cgcacactct ttctttttca
     1141 gacggttggc aggacggtgg ggcgaaatgc ccgctctgaa caggctctcg ttggcataaa
     1201 tgttgcccac accgaccacg accgcattgt ccatcagggc aagtttgacc gcgcgtttct
     1261 gcgccttcag cctcacatac agataatccg cacaaaatgc ctccgacaaa ggctccggcc
     1321 ccagtttttc caacagcgga tgatgttctt cgattccctc ataccaaagt atcgcgccga
     1381 actttctcgg atcgcggtaa cgcatgaccg tgccgtctga aaacacaata tcgacgtgat
     1441 cgtgtctgtc cggcctgccg atacgtccgt ccgacggcgt aaaaatccgc aagctgcccg
     1501 acatccccaa gtgaatcagc aaaatccccg tttggaaacg gacaatcaga tatttggcac
     1561 gcctgccgca ggacaaaacc tgccgaccgg acaaaatctc ccccaaatcg ggattgatct
     1621 gccagcgcag cttcaattgg cgcaatacca cggcttccac cgttttccct tcaatatgcg
     1681 gcgcgatgcc gcgcaacgtc gtttccactt ccggcaattc cggcataacc cctcccgaca
     1741 tttcttctga cagatgccgt ctgaaagacg gctgtcccta atccgcaatc cttgccgcac
     1801 ccgccgcaag ggctttgccg cccaaatacc cgtcccacgc gggcaggggc gttacgcccg
     1861 ccgccctttt gaccaaaacc aaaccgtaca ctgcggcaca ctgcggccac caacggtcgc
     1921 ccgccttttc cataaaccgc caaaagcgta tttgcccgag cgacgaaacg ggcggcagat
     1981 acaccataaa tttcccaaat tcaatatcga aaccgacatc cgcaagctgc cttttcaact
     2041 cgggcagcgg cagacagaag cgtttttccg gcaggcgttc gccgtcaaac caacggctga
     2101 atccccagag cgaatacgga ttgaaacccg tcagcatcaa acgtccggac ggtttcaata
     2161 tccggtgcgc ctcggacagg atttgcgaag gaacaccgcc ttcaagcgta tgcggaaaaa
     2221 gcagcatatc cgcagaaaca tccgccaaag ccatattttc cgccgacatc gacatatctc
     2281 gcggcacaca gacaacatct tcagacaggc tcagccacgg accgcccacc tgaaccgcac
     2341 acattcccga aaaacggtat gaatctagat accgcccgaa gaaatcctgt tccaattttg
     2401 caacataccg ccccatcgcc gtatcttcaa accatgaatc catattgcgt ccgtttcaaa
     2461 cagattgcct gccgatattc tattccaaac aggatttctg tcaaaaaaca catcggccgc
     2521 ccatttccga atccgcataa agttcctgta aaacttgacg ctttttcagt caaacagtac
     2581 catcggacga taaaatatgt ttattccgcc gaatataaat catgtccaaa ctcaaaacca
     2641 tcgctctgac cgcatcaggt ctgtccgttt gtccgggttt cctatacgcc caaaacacct
     2701 catcacacca aatcggtttg gcgattatgc gcttaaactc ttcaatactc gacctgcccc
     2761 cgacaaaaca atatttccaa tccggcagcc tgtggagcga gctgcgccaa ggcttccgga
     2821 tgggcgaagt caatcccgaa ctggtacgcc gccacgaaag caaattcatc gcaagccaca
     2881 gctatttcaa cagggtcatc aaccggagta gaccctatat gtaccatatc gccaacgaag
     2941 tcaaaaaacg caatatgccc gccgaagccg ccctgcttcc cttcatcgaa agcgcgttcg
     3001 tcaccaaagc caaatcacac gtcggcgcat caggattatg gcagtttatg cccgctaccg
     3061 gcaggcatta cggcctggaa aaaacaccgg tttacgacgg caggcacgac gtttacgccg
     3121 ccaccgatgc cgcactcaac tatctgcaat acctctatgg actgttcggc gactggccgc
     3181 ttgcctttgc cgcctacaac tggggtgaag gcaacgtcgg acgcgccatc aaccgcgccc
     3241 gcgcccaagg gctcgaaccg acctacgaaa acctgcgtat gcccaacgaa acgcgcaact
     3301 atgtccccaa gctgctcgcc gtgcgcaaca ttattgccac tccccaatct ttcggcatga
     3361 atatcagcga catagacaac aaaccgtatt ttcaggcagt cgaaccggat cgtccgctcg
     3421 acaacgaagc catcgcccgg cttgccggca tcacgcaaag cgagctgctc gccctaaacc
     3481 ccgcattcaa cgtccccgcg tttatcccca aaagcaaacg caaactgctg cttcctgtcg
     3541 cgtccgtaca aaccttccaa agcaactacc tcaacgccgc acccgacagc ctgttttcat
     3601 gggaagtcta tacgcctgcc gccaaaacca gcctgtccga catctcgacg gcaaccggca
     3661 tgagcattgc cgacatcaaa cgcctcaaca acctgaacgg caaccttgtc aacgcaggac
     3721 gcagcatcct tgtcgccaag aacggcaaaa cccttcagac ggcatcggaa tccgtcgttt
     3781 ccatcgacat cgacaatacg cccgacacct accgttccaa tatgccggca ggcacggtga
     3841 acgtcggcat tgcccgaatc cgacccgccg ccgcacagac agcggacatt accgtcgcac
     3901 ctttgccgca gaaaaccgtc cgtacggaac ccgatcccct tgtccgtatt gccgaacctg
     3961 cccttgcgac agccgcagcg caacctcaaa ccgaaaaaca gaccgccatg ccgtctgaaa
     4021 cccaaaccgc aacactcgcg cagatcatcc cccaaaacga catgcaggcg gcagatgaac
     4081 tcatgcagct tgttgcccga aacaacctgc gccgacaggc tgaagaaacc atctccgccg
     4141 tcatcggcac gcctgacaca gttgccgaac acaacatttc ctcatctccg caacataccg
     4201 ttgccgccga cggcaaacgc cgggcacgtt tggaaacgcg cgtagccaaa gctgccgacg
     4261 gcgaagccga aatctccccg ctccatgcca gcatccaccg cgttgtagaa ggcgacaccc
     4321 tgttcaacat tgccaaacgc tacaacgtca gcgtagccga cctgattgtc gccaacaaca
     4381 tcaaaggcaa caccatccaa aaaggacagg tactccgcct ggcgcaggca gcccctgccc
     4441 aaacccgtat tgaaaaagta tcctacaccg cgcgcaaagg cgacaccttc aaaagtatcg
     4501 ccgcgcgctt caatatccat atcgacgaca tccgccggct caatcccaac ctgaacacca
     4561 tcaatccggg acagagggtc aaactgattg gaagctgatt cggatacggc acatgacagg
     4621 actttctcag tcctgtcttt ttcatcccac atttcccata ccatcatgaa actcatcaaa
     4681 tacctgcaat atcaaggcat aggaagccgc aagcagtgcc aatggctgat tgccggcggt
     4741 tatgttttca tcaacggaac ctgcatggac gacaccgatg cagacatcga ttcctcatct
     4801 gtcgaaacgt tggatattga cggggaagca gtaaccgtcg ttcccgaacc ctatttctac
     4861 atcatgctca acaagcctga agattacgaa acttcgcaca aacccaagca ctaccgcagc
     4921 gtattcagcc tgttccccga caatatgcgg aacatcgata tgcaggcggt cggcaggctg
     4981 gatgcggata cgaccggcgt attgctgatt accaacgacg gcaaactgaa ccacaacctg
     5041 acttcgccga gcagaaaaat tcccaagcta tacgaagtaa cgctcaaaca ccccacagga
     5101 gaaacgctct gcgaaacctt gaaaaacggc gtgctgctcc acgacgaaaa cgaaaccgtt
     5161 tgtgccgccg atgccgtttt ggaaaacccg accaccctgc tgctgaccat taccgaagga
     5221 aaataccacc aagtcaaacg catgatcgcc gccgtcggca accgcgtgca acaccttcat
     5281 cgtcggcgat tcgcacattt ggaaacagaa aacctgaaac ccggcgaatg gaaatttatc
     5341 gattgtccaa aattctgaaa caaaattcca taatttccct atattcaact atatgcttat
     5401 taaataagta gttttacaaa aattaactca aaaaaaacag ttagcttgcg atataattcg
     5461 cttgtctgag taacgcctgt tcaagcaggc ttatgagtaa gacgttttcc ccgtaatgtg
     5521 ttttgccatc tatacttctc ccccttgtat agatggtttt tttatcttcg tttactacac
     5581 atactacata tgttactgac gcagcattcc cgatcatatg acatatgaaa ttttatttca
     5641 ccgtcccagt gtagaaaaat aaacgattca tctatctata aaatctgatt caatccttgt
     5701 tgatttatgc aaatcagaaa tatccactat aaaatataac tctattagtg aaaataacta
     5761 cacacttaaa aaacaatcag tcagttcctg ttaaaaaacg cgtgtagaat ttccgcttta
     5821 tctcaatcgg acacggtttc aatatgtctt ccccttcaaa taccaatcgt caaacctgat
     5881 ccagccgatt aacctatatc ctgaccgttg ccggcgcgac tgtcggtttc ggcgcgacgt
     5941 ggcgtttccc gtatttggtc ggtgaaaacg gtggtggcgc gtatgtgttt ttattctgta
     6001 tcgcgatgct ggttatcggc attccgatga ttttggtgga aaatgtcatc ggacggcgca
     6061 aaggcgtgaa cgcgctggat gcgttcggcg gtccgatgaa cggcaaaccc attgctaaaa
     6121 tttggaaact ggtcggctgg atggggctgc tcggcgcgtt cggcatcatg gcttattaca
     6181 tggcactcgg cggctgggta atcagctata tcgttaatat tattggagga aatttgaata
     6241 tttccagccc catcgacggt gtggttacaa aaggcttctt tgccgaacac attgaaacag
     6301 cccttgggaa attgcgtttt atacgctgct ttttgtcgcc gtgaaccaat ggattttggt
     6361 caaaggcgtt atcggcggca ttgaaaaagc ggcaaaatag ctgatgccgc tgctgttttt
     6421 gttcctaatc gcgatggtcg tccgcaacgt tacccttccg ggcgcaatgg aaggggttgc
     6481 tttctatctg aaacctgatt tcagcaagat taccgccgaa ctgttcgtct tcgttttggg
     6541 gcaggtattt tttgccctga gcctgggttt cggcgtgatg attaccttgt ccagctattt
     6601 ggataaaaac gaaaatctag ttcagacggc agttatcacg gcaattacca ataccatcat
     6661 cgccatactt gcgggcttta tgattttccc gtcgctcttc agcttcggcg ttgcccccga
     6721 ttccggcccg actttggtgt tccaaagctt gccgattgtg ttctcacata tgtgggcggg
     6781 atctgtgttc gccgtgattt tcttctcgct gctcctgatt gccgcgttga caacttcgct
     6841 gaccatttat gaagtgttga ttacgaccat tcaggaaaaa accaaaatcc gccgtaccgc
     6901 cgcgattacg attgtattgg ctgccatctt cattttcggc aacatcccgt ccattttgag
     6961 ctacggccct tggaaagatg tttccatatt tggcaaaaat atttttgacg cttttgacta
     7021 catcagcggc aacatcttgt ttatgctgac cgcgctcggt tccgcgctgt ttgtcggttt
     7081 tgtgatgaag gacgaagcga aggaagaatt gctttataaa ggcaaccata cgacggtcaa
     7141 tatttggttt gcctatgtga aatacctcgt gccgttggtg attctgctga tttttatcag
     7201 caacctgttc taatccgcag caatcaatgc cgtctgaagg tcatacctct ttcagacggc
     7261 attgcattga tgcccgccac aatacccgca acgccggaat ccgaaccggc ttgccggctt
     7321 ctgtcggatg tttccgggca ggcggcgttc cgtctgctta gacaatcgtc ctttaaaaca
     7381 ggtagaatcc gccccaacgg gaaacacatc cttcagacgg caaaacccat accccaaacc
     7441 atcaggaatc ccccttatga acagacaaaa agtcatcgcc atcgacggcc cgggcgcatc
     7501 gggcaaaggc acggttgccg cccgcgttgc cgccgcattg ggatacgatt atctcgatac
     7561 cggcgcactc taccgcctga ctgccctata tgcacaaaaa caaggcgtgg gatggcacga
     7621 tgaagaaaac gtttccgaac tggcaaaaaa actgcccgcc gtattttcag gcagccgcat
     7681 cctgctcggc ggcgaagacg tttcagacgg catccggaca gaagccatcg gcatgggcgc
     7741 atccgcagtc gcacagttgc ctaaagtccg cgccgccctg ctgcaacgcc aacgcgattt
     7801 tctgaccgaa aaaggactgg ttgccgacgg acgggacacc ggatcggtcg tcttccccca
     7861 agccgaactt aaaatcttcc tgacggcaga atccaaaatc cgtgccgaac gccgcgccaa
     7921 acaaatcggc atcccctgcg aaggtttggc attcgagcgc atcctgtccg acatcgaagc
     7981 cagagacgag gcagaccgaa accgcaaagt tgcccccctg aaacaacagc ccgatgccct
     8041 gcttttggac acaagccgcc tgactataga agaaactgta aaaaaagtgc ttgattggta
     8101 tcgtgaagtt taaattttca ggtataatcg cacaaattac gtttcagacg gcataaaaat
     8161 cccccatatg ccgtctgaaa ccttatgtac ccgtctgccc tgccaaggac ggcagatatc
     8221 caccaaccca ccccgcaccc cttggcggtg taccgaaaag agttatatat gtctatggaa
     8281 aattttgctc agctgttgga agaaagcttt accctgcaag aaatgaaccc gggtgaggtg
     8341 attaccgctg aagtagtggc aatcgaccaa aacttcgtta ccgtaaacgc aggtctgaaa
     8401 tcagaatccc tgattgatgt agctgaattc aaaaacgctc aaggcgaaat tgaagttaaa
     8461 gtcggcgact tcgttaccgt taccatcgaa tccgtcgaaa acggcttcgg cgaaaccaaa
     8521 ctgtcccgcg aaaaagccaa acgtgcagcc gattggattg ccctggaaga agccatggaa
     8581 aacggcgaca tcctgtccgg catcatcaac ggaaaagtca aaggcggcct gaccgttatg
     8641 attagcagca tccgcgcatt cctgccgggt tctttggtcg acgtacgtcc tgtaaaagac
     8701 acttctcact tcgaaggcaa agagatcgaa ttcaaagtga tcaaactgga caaaaaacgc
     8761 aacaacgtcg ttgtttcccg ccgcgccgtt ctggaagcca ctttgggtga agaacgcaaa
     8821 gccctgctgg aaaacctgca agaaggctcc gtcatcaaag gcatcgttaa aaacattacc
     8881 gattacggtg cattcgttga cttgggcggc atcgacggtc tgttgcacat caccgatttg
     8941 gcatggcggc gcgtgaaaca cccgagtgaa gtcttggaag tcggtcagga agttgaagcc
     9001 aaagtattga aattcgacca agaaaaacaa cgcgtttcct tgggtatgaa acaactgggc
     9061 gaagatcctt ggagcggtct gacccgccgt tatcctcaag gcacccgcct gttcggcaaa
     9121 gtatccaacc tgaccgacta cggcgcattc gtcgaaatcg aacaaggcat cgaaggtttg
     9181 gtacacgtct ccgaaatgga ctggaccaac aaaaacgtac acccgagcaa agtcgtacaa
     9241 ctgggcgacg aagtcgaagt catgattttg gaaatcgacg aaggccgccg ccgtatctct
     9301 ttgggtatga aacaatgcca agccaatcct tgggaagaat ttgccgccaa ccacaacaaa
     9361 ggcgacaaaa tctccggcgc ggttaaatcc attaccgatt tcggcgtatt cgtcggcctg
     9421 cccggcggca tcgacggttt ggttcacctg tccgacctgt cctggaccga atccggcgaa
     9481 gaagccgtac gcaaatacaa aaaaggcgaa gaagtcgaag ccgtcgtatt ggcaatcgac
     9541 gtggaaaaag aacgcatctc cttgggtatc aaacaactgg aaggcgatcc gttcggcaac
     9601 ttcatcagcg tgaacgacaa aggttctttg gttaaaggtt ccgtgaaatc tgttgacgcc
     9661 aaaggtgctg ttatcgccct gtctgacgaa gtagaaggct acctgcctgc ttccgaattt
     9721 gcagccgacc gcgttgaaga tttgaccacc aaactgaaag aaggcgacga agttgaagcc
     9781 gtcatcgtta ccgttgaccg caaaaaccgc agcatcaaac tttccgttaa agccaaagat
     9841 gccaaagaaa gccgcgaagc actgaactcc gtcaatgccg ccgccaatgc gaatgccggc
     9901 accaccagct tgggcgacct gctgaaagcc aaactctccg gcgaacaaga ataaggttgc
     9961 agacatgaca aagtctgagt taatggttcg tttggcagaa gtgtttgccg ccaaaaacgg
    10021 cacgcatctt ctggcaaaag acgtagagta cagcgtaaaa gtcttggttg acaccatgac
    10081 tagatcgctt gcccgaggtc aacgcatcga aatccgcggt ttcggcagct tcgatttgaa
    10141 ccatcgtcct gcccgcatcg gtcgcaatcc caaaaccggc gagcgtgtgg aagtacctga
    10201 aaaacatgta ccccacttca agcccggtaa agaattgcgc gagcgggtcg acttggcttt
    10261 aaaagaaaat gccaattaaa ccttagcatc aaaacgccgc tgttacgcgg cgttttttct
    10321 gtggtttaac ttcatccgct gcttcaatac cttgagccaa gcaagcaacg gattagagcg
    10381 tggatttttt atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag
    10441 agaacaattc tctaaggtgc tgaagcacca agtgaatcga ttccgtacta tttgcactgt
    10501 ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatatcattg cttacaatcc
    10561 gctttttaaa caacaaattt ttgatttcta ttacgaacag gacaaaaatc ctgcttattg
    10621 cactaaaact aagccgtttc aggaatttgc ggcaaattta cggcttttac cgaacctaat
    10681 gctttcgctt tttggtaaaa cgccaatttg tattcaagca aatctaaata gcgttttaat
    10741 tcggcaattt gacacttcac attttctatt tgattttcaa acaaggaaag gcgttcttca
    10801 atggtatcgt cgccaatgac ggtacattcc gcaaagcgtt tgatgtcttt taagctcatt
    10861 cccgtatttt tcaagcattg caataagccc aaccattgca aatcgttatc ggtaaaacag
    10921 cggttaccgt attcatcacg tccgatattg ggcaacaaac cttctttgtc gtaaaaacgt
    10981 agggtgtggg cggagatgcc tattttttcg gcggctttgg cagtagtata agtcattttc
    11041 cctccttcta aacaaaaaca gtaaaaaaca cttgctttag agttaactct aaagtgtaaa
    11101 ctgttgctat gttgctcagg caaggcaact ttgtcaatga attaagagga aagacaatgg
    11161 aaatgaaaca agccgattca accatcaaat ctcgtgcggc ggtggcattc gcccctaacc
    11221 aacccttaca aattgtggaa atcgacgtag aaatgccgcg taaaggcgag gtgttaatcc
    11281 gcaataccca cactggcgtg tgccatactg atgcgtttac gttatcagga agcgatcctg
    11341 aaggcgtatt ccctgtggtg cttggacacg aaggtgcggg tgtggtcgtt gctgtgggcg
    11401 aaggtgtgtc aagcgtaaaa ccgggtgatc acgtgattcc gctttacacc gccgaatgtg
    11461 gcgaatgtga gttttgttgt tcaggtaaaa ccaacttgtg cgtctcagtg cgtgatacac
    11521 aaggtaaagg cttaatgccg gacggcacga cgcgtttttc ttatcaaggt cagccgattt
    11581 atcactatat gggctgttcg actttcagtg aatactccgt tgttgccgaa gtttcactgg
    11641 cgaaaatcaa ccctgaagcc aaccatgaac aagtatgttt gctcggctgc ggcgttacca
    11701 caggtattgg tgcggtacat aatacggcaa aagtgcaaga aggcgactct gttgccgtgt
    11761 ttggcttagg cgcgattggt ttggcggtgg tgcaaggtgc gcgtcaagcc aaagccggcc
    11821 gcattatcgc cattgatacc aatccatcaa aatttgagtt ggcaaaacag tttggtgcaa
    11881 cggattgttt gaacccgaac gattacgata aaccgatcaa agatgtgttg ttagacatta
    11941 ataaatgggg cattgaccat acctttgaat gtatcggcaa cgtaaacgta atgcgtcagg
    12001 cattagaaag tgcacatcgt ggttggggtc aatccattat catcggcgta gcaggtgcag
    12061 gacaagaaat ttcaacgcgt ccgttccagt tggtaacagg tcgtgtttgg aaaggttcag
    12121 catttggcgg tgtgaaaggt cgctctgaac tgccgaaaat ggtggaagat tcaatgaaag
    12181 gcgacattga gttagaaccg tttgtaaccc acacaatgac actcgatcaa atcaataaag
    12241 cctttgactt aatgcacgaa ggtaaatcga tccgcgccgt tattcactac taaggtatgc
    12301 gatgaaactg attgaacaac atcaaatttt tggcggttcg caacaagttt gggcgcatca
    12361 tgcccaaacg ctgcaatgcg aaatgaaatt tgccgtctat ttgccaaata atccagaaaa
    12421 tcgaccgctt ggtgtgattt attggctttc cggcttgacg tgtacggaac aaaatttcat
    12481 taccaaatca ggctttcagc gttatgcggc agaacatcaa gtaattgtgg tggcctccga
    12541 tagcagccct cgcggagagc aagtgccgaa cgatgatgct tacgatttag gacagggtgc
    12601 aggcttttat ttgaatgcga ccgaacagcc ttgggcggcg aattatcaaa tgtatgatta
    12661 cattttgaac gagctgcccc gtctgattga gaaacacttt cctaccaacg gcaaacgttc
    12721 cattatgggg cattcaatgg gcggacacgg cgcattggta ttggcgctgc ggaatcagga
    12781 acgttatcaa agtgtttctg ccttttcgcc tattttatcg ccaagcctcg tgccgtgggg
    12841 agaaaaagcc tttactgctt atttagggaa agaccgtgaa aaatggcagc aatatgatgc
    12901 gaactcactc attcaacaag gctataaagt gcaaggtatg cgcatcgatc aaggcttgga
    12961 agatgagttt ttgccgacac aattgcgtac cgaagatttt atcgaaacct gccgtgcggc
    13021 aaaccagccg gtcgatgtgc gtttccataa aggctacgat cacagctatt acttcatcgc
    13081 cagttttatt ggcgagcata ttgcttatca cgccgcgttt ttgaagtaaa ccaaagagcg
    13141 ttcagtgttc aaagcagttt tgggatagcc ggcacgaggg cggtaagaag tgccggcata
    13201 aacgtatgcc gtctgagccg gaaggagccg actctacgg
//
LOCUS       DAWEWP010000046        12880 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWEWP010000046 DAWEWP010000000
VERSION     DAWEWP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.046"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..325
                     /locus_tag="WGN53_000943"
     CDS             <1..325
                     /locus_tag="WGN53_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139953.1"
                     /translation="AEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTSFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(497..913)
                     /gene="gloA"
                     /locus_tag="WGN53_000944"
     CDS             complement(497..913)
                     /gene="gloA"
                     /locus_tag="WGN53_000944"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ0139954.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAIEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(960..2129)
                     /gene="lapB"
                     /locus_tag="WGN53_000945"
     CDS             complement(960..2129)
                     /gene="lapB"
                     /locus_tag="WGN53_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ0139955.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2140..2454)
                     /locus_tag="WGN53_000946"
     CDS             complement(2140..2454)
                     /locus_tag="WGN53_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ0139956.1"
                     /translation="MKLIYTFIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            2764..3762
                     /gene="ilvE"
                     /locus_tag="WGN53_000947"
     CDS             2764..3762
                     /gene="ilvE"
                     /locus_tag="WGN53_000947"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ0139957.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETEAYLDALVE
                     LVKRAADEIPDAPSALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(3948..4733)
                     /gene="fabI"
                     /locus_tag="WGN53_000948"
     CDS             complement(3948..4733)
                     /gene="fabI"
                     /locus_tag="WGN53_000948"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ0139958.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4888..5709)
                     /gene="dapD"
                     /locus_tag="WGN53_000949"
     CDS             complement(4888..5709)
                     /gene="dapD"
                     /locus_tag="WGN53_000949"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ0139959.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPTGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5719..5949)
                     /locus_tag="WGN53_000950"
     CDS             complement(5719..5949)
                     /locus_tag="WGN53_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139960.1"
                     /translation="MPSERRRRGIKVLPPFCIDSGAEKAIPAARPIPKRQCSATACVL
                     LTNMRLRGTIAHSFQMNILPENTGKRPTKHLT"
     gene            complement(5919..7562)
                     /gene="pgi"
                     /locus_tag="WGN53_000951"
     CDS             complement(5919..7562)
                     /gene="pgi"
                     /locus_tag="WGN53_000951"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0139961.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYVDGRDVLPEIRRELNRALEFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSSVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGTIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            8072..9304
                     /gene="pilG"
                     /locus_tag="WGN53_000952"
     CDS             8072..9304
                     /gene="pilG"
                     /locus_tag="WGN53_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ0139962.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9378..10238
                     /locus_tag="WGN53_000953"
     CDS             9378..10238
                     /locus_tag="WGN53_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ0139963.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKKHLNLPLTEKESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10240..10872
                     /locus_tag="WGN53_000954"
     CDS             10240..10872
                     /locus_tag="WGN53_000954"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ0139964.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFVSLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLCEKTMRLHAFYSGIFASKPTQGKHND"
     gene            10865..11074
                     /locus_tag="WGN53_000955"
     CDS             10865..11074
                     /locus_tag="WGN53_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ0139965.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSSQTESLPEISEPDMAYR"
     gene            complement(11159..12835)
                     /gene="pilB"
                     /locus_tag="WGN53_000956"
     CDS             complement(11159..12835)
                     /gene="pilB"
                     /locus_tag="WGN53_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ0139966.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         2955 a         3575 c         3378 g         2972 t
ORIGIN      
        1 agcggaaatc actttggaaa cgcctgattc caacacctct gaggcagacg ctttgcccga
       61 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
      121 tatcccaaat aatgcagata ccagtttccc ttcggaatct gtaggttctg acgcgccttc
      181 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
      241 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
      301 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
      361 ttcagacggc atcttttttg ccttatcggt gtaacggata aagtttgaac cggcacaggc
      421 tcaaacagca ggccgacggc aacaaaatgc cgtctgaaac ccctaaaggc ttcagacggc
      481 attggcggcg gcggtatcag gcagtttgat aggcaaccga atcgtcaccg cttttctttt
      541 gaatgaactc gattttgtat ccgtcggggt cttcgacgaa ggctatcacg gttgtgccgt
      601 gtttcatcgg gccggcttcg cggacgacgt ttccgccctg ccgcttcaca cgttcgcagg
      661 cttcgtaggc atcgtccact tcaatcgcga tgtgtccgta agcgttgccc aagtcgtatc
      721 gttccgtatc ccagttgtgc gtcagttcca aaaccgtgct gtcggtttca tcgccgtaac
      781 cgacgaaggc aagggtaaat ctgccttcgg gatagtcttt tcggcggagc agtttcatgc
      841 ccaaaacgtt ttggtagaaa tcaagggatt tttcgagatt gcccacgcgg agcatagtat
      901 ggagtaagcg catttttcgt gttcctcatt ggtggtggtt aacttcgatt tagtggtggt
      961 taaacttcaa ttttattcgg ggtaaacgtc tgccatttgt tgcaggcggg acagtgccag
     1021 aaaaagactt gggatttgaa gtggcagttg cggcaacggt acatcacgct gcgctgtagc
     1081 tgccgtccga taaccgaacg catcatgtcg gcatcggctt tccaagccgg attcatatcg
     1141 ctgagtttca aaccgagcag gcggtacacg ccgttaaggt cgggcttgcg gcggacaagc
     1201 tcgacggcgg tttgcgcggc ttctttctcg cccttaagca gcagggattt ctcgtacacg
     1261 acattgatca ggtcaagttc gggaaacgtc tgcatatatc ctgtcagacg gttcaagcct
     1321 tcttcaggtt ttccctgcgc ggcataggct tcgtaaagct tctcgccgac catgctcaag
     1381 tatgcatggt tttgctgctc gatggcggca taggcttcga cggcggcagg gaaattgcct
     1441 tgtcggtgtt cgatgtcgcc caaaatcatg ttggcgcggg tgcatttttt gttggcttcg
     1501 agtgccttgc cgacattgaa acgcgcgaca tcgaaattgg acttgaacag cgcggcttgg
     1561 gcaagttcgc aataaaactg ggcaatttca aactgatagg tctgatcgtc atggctgagc
     1621 agccgggcgg tttcaaccgc tttttcccaa tccctgtcct gttggtagat attgagcagg
     1681 tgctgtctgg cttcacgcgc catttcaccg tcttgcagcc ccaaaaaaat ctgttcggca
     1741 cgatcgacca aacccgcgct ttggtagttt tgcgccaatt caaacaggac gcgcgcgcgc
     1801 ttttcgccga ccgtatcggg agaatcgagc attgtccggt gtatgttgat ggctttgtcg
     1861 ttttcgccgc gctggcggta aagtttgccg agggtgaggt tcaaatcata cgattgcggc
     1921 cggccgtcga cgacttccgc caactccctt gccgcgcgcc cgctgttgcg gtcgaccaaa
     1981 gcgtccaagc ttttataaaa tcccgaaggg atgctttttg cctgcttcaa tacggttttc
     2041 atatccacgc gggcggcaaa ccagcccatc gcgaagaaga cgggcaaaag gataatcggc
     2101 agcaggataa tccacaattc gttgtccata tcggctttct taaggctgtt tggtagattc
     2161 gggcgcattt tgcgccggtg gtgcggtcag ctccttcccc gtcaaacgcg catttttctt
     2221 tacttcggca cgcaacctgc cgttctcgcc acgtaacgac aacaaccgtc cgaacaaggc
     2281 aaacattcca aaaataatac cgactacaaa tgcgccgaac aatacgacaa tcagcggcaa
     2341 atcgaatttt tgccccggca ggtaggaaaa ggtaacggca tccgtattaa taacggcaag
     2401 cagcaggaag agcagcagga taatgatttt gatgaaggta tagataagtt tcatgtttgc
     2461 tccgtaaata aaaacgccgc ccggacagct tcgcactctg aaccaagatt gcgctagttt
     2521 aaacgatttg cacggtttcg gataggatgc ggcagcgtgt tcagacggca tacggagtat
     2581 gcgattgccg acaattttac caaatacatc gttcctttcc atttgaaaaa taacggattg
     2641 gacaccgcat cgacagaaaa acccgccgcg cacttgtcaa aaccctgttt gcaggcgtat
     2701 ctttacaatc ttcaaattca aaccgttcat tgaaacatat cagaataaga aaggctttac
     2761 atcatgagca gacccgtacc cgccgtattc ggcagcgttt ttcacagtca aatgcccgtc
     2821 ctcgcctacc gcgaaggcaa atggcagccg accgaatggc aatcttccca agacctctcc
     2881 ctcgcaccgg gcgcgcacgc cctgcactac ggcagcgaat gtttcgaggg actgaaagcc
     2941 ttccgtcagg cagacggcaa aatcgtactg ttccgcccga ccgccaacat cgcgcgtatg
     3001 cggcaaagtg cggacatttt gcacctgccc cgccccgaaa ccgaagctta tctcgatgct
     3061 ttggtcgaac tggtcaaacg tgccgccgac gaaattcccg atgcaccttc cgcgctgtac
     3121 ctgcgcccga ccttaatcgg taccgatccc gtcatcggca aggccggttc tccttccgaa
     3181 accgctctgc tttatatcct cgcctccccc gtcggcgact atttcaaagt cggctcgccc
     3241 gtcaaaattt tggtggaaac cgaacacatc cgctgcgccc cgcatatggg acgcgtcaaa
     3301 tgcggcggca actacgcttc cgccatgcac tgggtgctga aggcgaaagc cgaatacggc
     3361 gcaaatcaag tcctcttctg cccgaacggc gacgtgcagg aaaccggcgc gtccaacttt
     3421 atcctgatta acggcgatga aatcattacc aaaccgctga ccgacgagtt tttgcacggc
     3481 gtaacccgcg attccgtact gacggttgcc aaagatttgg gctataccgt cagcgaacgt
     3541 aatttcacgg ttgacgagct caaagctgcg gtagaaaacg gtgcagaagc gattctgacc
     3601 ggtacggcag ccgtcatctc gcccgttact tccttcgtca tcggcggcaa agaaatcgaa
     3661 gtgaagagcc aagaacgcgg ctatgccatc cgtaaggcga ttaccgacat ccagtatggt
     3721 ttggcggaag acaaatacgg ctggctggtt gaagtgtgct gatgctttaa ataaaaaaaa
     3781 tgccgtctga aacccgtttg gcgtttcaga cggcattttc gcgttcggac catttccgct
     3841 gcacctgcag caagtcgggc atttccctag aaatacgctt cagacggcaa accgccgccc
     3901 gaaaccgata cgcggtacgg acggcgcacg gatttgaaaa cggcggatta tccctcggtg
     3961 ctcaaggcat taatgctgta accgccgtca acgtaagtga tttcgccggt aatgccggac
     4021 gacaggtcgg acagcaggaa ggcggcggta ttgccgactt cttcgatggt aacgttgcgg
     4081 cggagcggat tgtgggcggc gacgtgtccc aagagtttgc cgaaatcggc gatgccggag
     4141 gcggcaagcg ttttgatcgg gcctgcggaa atgccgttgc agcggatgcc ttctttgccc
     4201 agacaggcgg cggtaaaacg gatgcccgcc tcaaggctgg cttttgccat acccatcacg
     4261 ttgtaattcg gaatcgcgcg caccgcgccc aagtagctca gggcaacgat ggcggagttt
     4321 ctgccgcgca tcatcggacg ggcggctttt gccaatgcgg gcaggctgta tgcggaaatt
     4381 tcgtgtgcgg tgttgaacgc ttcgcggctg atgctgtcga ggaagtcgcc gctcaaggct
     4441 tctttcggcg caaaaccgat ggaatgcacc aaaccgtcca agccgtccca atgtttgccc
     4501 aaatcggcga acacttggtt gatttcgtcg tcgctggcga catcgcagcg gaacacgagt
     4561 tcggaatcca attccgccgc cattttgcgg acgcgctctt ccagtttgtc cacaacgtag
     4621 gtaaacgcca gttccgcgcc ttgttcgcgg caggctttgg cgatgccgta agcgatggaa
     4681 cgctcggaaa tcatgccggt aatcagaatt tttttgcctt gcagaaaacc cattttgtat
     4741 ccttcaaata gtgttcggtt cgttctaaac cgttgattat agcaaattgt ccctgtttct
     4801 gtgttttcac gttgcagcat gcaaacggca atgccgtccg aagcggattt cagacggcat
     4861 cggacgtttc aaatacggtt taaggcatca gatgccgcgc aacaattcgt tcacgctggt
     4921 tttcgcacgg gtttgcgcgt ccacgcgttt gacgatgacg gcgcagtaaa ggctgtggct
     4981 gccgtctttg gaaggcatac tgccggacac gacgaccgaa cctgtcggta cgcggccttg
     5041 gtagatttcg ccggtggtgc ggtcgaagat tttggtggat tgaccgatga acacgcccat
     5101 cgaaatcacg ctgccttctt cgacaatcac gccctcaacg atttcagaac gcgcaccgat
     5161 gaagcagttg tcttcaatga tggtgggggc ggcctgcagg ggttcgagta cgccgccgat
     5221 accgacgccg ccactcaagt gcacgttttt accgatttgc gcgcaagagc cgacagtcgc
     5281 ccaagtatca accatcgcgc cttcgtcgac gtatgcgccg atgttgacgt aagacggcat
     5341 cagcacgaca tttttcgcca caaagctgcc gcgtcgggca accgcacccg gaacggcgcg
     5401 gaagcctgcg tttttgaact cgtcttcaga ccagtcggca aacttggtcg gcactttgtc
     5461 gaagtatttg ttcacgccgt cgttgaggac ttcgttgtct tggatgcgga aggacagcaa
     5521 cacggctttt ttcgcccatt cgttgacttt ccactcaccc acgcccaagc gttcggcaac
     5581 gcgcagtttg ccggaatcga gttggcggat ggtttccaac acggcttctt tgacttcggg
     5641 agtaacggtg gtcggggtga tgtccgcgcg gttttcaaag gcggtttcga tgatgttttg
     5701 caaagacata atgtttcctt atgtgagatg tttcgtggga cgcttgccgg tattttcggg
     5761 cagtatgttc atttgaaatg aatgcgctat tgtgccacgc aaacgcatat tcgtcagcaa
     5821 tacgcaggcg gttgccgaac attggcgttt cggaatcggg cgggcggcag gtattgcctt
     5881 ttccgcgccc gaatcaatac agaaaggcgg cagtactttt atgccgcgcc gccgcctttc
     5941 agacggcatt cgcggtaaaa cgccatcagc ccttcggtcg aggcatcgtg tacggacgtg
     6001 ccgccttcca gttcgccgat gatggttttt gccaactgtt tgccgtattc caccccccac
     6061 tgatcgaagg ggttgacgtt ccatatcgtg ccttggacga aggttttgtg ttcgtaagcc
     6121 gccatcagca tacccagatt gtagggcgtg aggcggtcga gcaggatgct gttactgggg
     6181 cggttgccgg ggaactcttt gtgcggcgcg aggcgttcgc gttccgcttc gggcaaatct
     6241 gccaattcgg cgcgtgcttc gtccaaggtt ttgcccttca tcagggcttc cgcttgggca
     6301 aaggcgttgg caacggtaaa acggctgcgt ccgtcctcca cgccctgcgc cgtcatcggg
     6361 acgataaagt cgcagggaat caggcgcgtg ccttgatgaa gcagttggaa ataggcgtgc
     6421 tggcagttga ccccttcacc gccgaacacg atgccgcccg ttttgcacac ggcgggactg
     6481 ccgtctgaag cgcggctttt gcccaaactc tccatatcga gctggttcag ccacaccggc
     6541 agcaggcgca ggttgtggct gtacggaacg gcggtctgcc cgtccgcgtg ttggaaattg
     6601 ttataccaca cggcaatcag tgccatcaga acgggaatgt tgcggcgcgg cggcgtgtgg
     6661 aaaaaatggc tgtccatcgc gtgcgccccc gccaacaact cgcggaaacg cgccccgccg
     6721 accgcaacca tcacgggcaa accgacggac gaccagacgg aatagcgtcc gcccacccag
     6781 tcgtacatcg caaacacgcg ttccgccgcg ataccgaaag ccgcagctgc cgcagtgtcg
     6841 gcagacaccg cgcaaaaatg gcacgccgtt tcggattccg agaaccccgc accgcgatac
     6901 cacgccttga ctgcctgtgc attgagcagg gtttccggtg ttttgaagga cttgctggca
     6961 acgcaaaaca ctgtcgtttc ggggttcaga cggcataaaa ccgcatccag gcaggcagga
     7021 tcggcgttgg cggcaaaatg gacggcgata tgccgtctga acggctcgag tgcctgaacg
     7081 cacattgccg gcccgaggtc ggatccgcct atgccgatgt ggacaaaatc cgtaatccgt
     7141 ttcccagtta tcccctgata cgaaccgtcg tccaaactgt gtgcaaactc caacgcacga
     7201 tttaactcgc ggcggatttc gggcaacacg tccctgccgt ccacataaac ggcatccgca
     7261 ccgtcgggca ggcgcaaagc cgtatgcagc gcggcacgcc cctcgctgcc gttgactttc
     7321 gcacccgtcc gcaaagcacg cattttccct tccaaatccg ccgtctccgc aagacggcag
     7381 agcagttgca gcgtatcttc gcccaaacgg tttttgctgt aatcgaacaa catcccgtcc
     7441 aaacgctcat gcatacgctc aaaacggtcg ggttcgcagg caaagcggtc gcgcaaaagg
     7501 acatgacgcg tatcctgata atggcgttcg agcgcatacc atgcacgggt aaaagcattc
     7561 atctgttttc cttgattttt cagaaccgga ttaaaatgta gcagaatgta gtttaacaaa
     7621 cggcagcggc tttggcgaat ctccggaaca ccgcccgccg cgtccaatag caccttattt
     7681 ccatatggcg aaaatatagt gaattaacaa aaatcaggac aaggcggcga gccgcagaca
     7741 gtacaaatag tatggaaccg attcacttgg tgcttcagca ccttagagaa tcgttctctc
     7801 ttgagctaag gcgagccaac gctgtaccgg tttttgttaa ttcactatac cctccggccg
     7861 acatttgacc cgcaacaata tcctgcaaga tttattgtgt acgcataaat gccggacagc
     7921 cgcctaaata aggtataatc gcatccgatt ctgtcccgtt tgccgcccgc aggcgggatg
     7981 tcggcggttt tcatcttacc gggaacacgc cggtactgtt ttttaacgat gtttcttaca
     8041 ttttattcca attactttac ggggctagaa tatggctaaa aacggaggat tttctttgtt
     8101 cgcaaagaaa gaaaaacgct ttatctttga aggcaggcat tccgcctccg acaaactggt
     8161 caacggcgaa gtatccgcgt ttaccgaaga agaggcgcgc aaaaaactgg caaaacgcgg
     8221 catccgcccg ttgcagatta cccgtgtgaa aacaagctcc aagcgcaaaa tcacacaaga
     8281 agacatcacc gttttcaccc gccagctgtc cacgatgatt aaagcgggcc tgccgctgat
     8341 gcaggcattt gaaatcgtgg cgcgcggaca cggcaacccg tctatgacgg aaatgctgat
     8401 ggaaatccga ggcgaagtgg aacagggcag ctcgttgagc cgcgcattct caaaccaccc
     8461 gaaatatttc gaccgcttct actgcaacct ggttgcggca ggcgaaacgg gcggcgtatt
     8521 ggaaagcctg ctggacaaat tggcaattta caaagaaaaa acccaggcca tccgcaaaaa
     8581 ggtaaaaacc gcactgacct atccggtatc cgtcatcgcc gtcgccatcg gtttggtatt
     8641 cgtgatgatg attttcgtac tgcccgcctt taaagaagtt tacgccaata tgggcgcgga
     8701 gcttcccgca ctgacccaaa cagtgatgga tatgtccgac tttttcgtct catacggctg
     8761 gatggtgctg atcgcactgg gctttgccat atacggcttc cttaaattga aggcgcgttc
     8821 gattaaaatc caacggcgta tggatgccat actgctgcgt atgccgattt tcggagacat
     8881 tgtccgcaaa gggacgattg cccgctgggg caggacgaca gcgacgctga ttgcggcagg
     8941 cgtgcctttg gtcgatgtat tggactccac tgccggcgcg gcgggcaatt taatctatga
     9001 agaagccacc cgggaaatcc gtacgcgcgt catccaaggt ttgtctatga cttcggggat
     9061 gcgtgcgacg gaactgttcc ccaatatgat gttgcagatg tcttccatcg gcgaggaatc
     9121 gggttctttg gacgatatgc tcaacaaagc cgccgaattt tacgaagacg aggtggacaa
     9181 tgcggtcggc aggctgtccg ctatgatgga gccgatcatt atcgtgattt taggcttggt
     9241 catcggcacg cttctggtcg ccatgtatct gccgctgttt aatttgggca acgtggtcgc
     9301 ctgatttgcc gcacagatcc ggcgcggatt cgctctgcgc cggtttgttt ttgttttgaa
     9361 tacattgaga acaaaatatg tctgatttgt ctgtattgtc gccgtttgcc gtgcctttgg
     9421 cagcggtgtt cgggctgctg gtcggcagtt tcttaaacgt cgtcatttac cgcgtgccgg
     9481 tcatgatgga acgcggctgg acggtatttg ccaaaaaaca tttaaacctg ccgctgaccg
     9541 aaaaggaaag ccgtaccttc aacctgatga aaccggattc ctgctgtccc aaatgccgcg
     9601 tgccgatacg cgcgtggcag aacatcccga ttgtcagcta cctgctcctg cgcggcaaat
     9661 gcgcttcctg ccaaaccaaa atcagcatac gttatccctt aatcgagctg ctgaccggcg
     9721 tattattcgg gctggtcgcc tggcaatacg gctggtcttg gattacattg ggcggtttga
     9781 tactgaccgc gtttctgatt tccctgacct ttatcgatgc ggacacccaa tacctgcccg
     9841 actcgatgac actgcccttg atttggctgg ggctgatatt taatttggac ggcggcttcg
     9901 tgcctttgca gtctgccgtt ttaggtgcgg ttgccggcta tggttcgata tggcttttat
     9961 gcgcggtata taaactgatt aaaggcgaaa tcggtatggg caacggagat ttcaaactga
    10021 ttgccgcatt gggcgcgtgg ctcggcatat ccgcattgcc cgtgctgatt tttgtttcct
    10081 cgctgatcgg tttggtcgcg gcaatcgtta tgcgcgtcgc caaggggcag cattttgcct
    10141 tcggccccgc actgacagtt tcgggctgga taatttttac ggcaaacgat tccgtatggc
    10201 gggcggtcaa ctggtggctg acccatccgg tgctgtaaga tgacggtatg ggtcgggctg
    10261 accggcggaa tcggcagcgg caaatcggca gccgcgcaat gttttgccga tttgggcgtg
    10321 ccgcgcatcg atgcggacgc ggcggcgcac tcgctgacgg cttcagacgg catcgccctg
    10381 ccggaaatca ggcggctgtt cggcgacacc gtttttgaca cacagggttt gttgcggcgc
    10441 gacatattgc gtaaagaagt ctttgcctcc ccatcgcgaa aagccttgct cgaatccgtg
    10501 atgttgccgc tgattttctc agaaatcaaa aaacagcaag aaacctttac cgatgcggtt
    10561 tacggcattg tcgaaattcc gctgctgacg gaaaagcgtc aatttgtcag cctgatacgg
    10621 cgtgtcctga ccataagcgc acctttggaa aaacgtatcg gcagggtgat ggcccgcagc
    10681 gggctgacgc gcggcgaggt ggcggacatc atcagccatc aggcatccga atccgaacgc
    10741 ctgctgcttg cagacgatgt gctgctcaat gacggcagcc tcaaaagcct gtgtgagaaa
    10801 acaatgcgcc tgcacgcgtt ttattcaggg attttcgcct caaaaccaac acaaggaaaa
    10861 cacaatgact gaatcgcggc aaacacgcct tcaagtcaaa tgtccgacct gtcaaacggc
    10921 agtagtatgg aaaccggaaa acgcattccg ccccttctgt tcgcaacgct gcaaactgat
    10981 cgacttgggc ggatgggcag acgggaaata tacggtttcc agccaaacgg aaagtttgcc
    11041 ggaaatatcc gaacccgaca tggcataccg ctgaccgccc cgccttcccg gcaaacaccc
    11101 tgaaagtcaa atgccgtctg aaacaaacac gcttcagacg gcattttcat tctcaaacct
    11161 aatcgttggt atttgccgtt acctcttcca atgaagtaat gccctgcata actttcaaaa
    11221 taccggcccg gcgcaaatcc accataccct ccttataggc aacgtccaaa atatccactt
    11281 ccgtaccgtt gttcataatc acacgctgca tttcttcgct gatgggcata acctcataca
    11341 cgcccgcacg ccccttataa ccctgccccc ggcaacggtc gcaaccgacg gcgcggtaaa
    11401 gtttccaatc ttttgcaagg tcctcatcgg tgaagccgac ttccttcaaa gcagaggcag
    11461 acgggcgttc cacttcctgt ttgcagctcg aacacagcct gcgtaaaaga cgctgcgcca
    11521 taatcaggct gaccgaactg gcaatattaa acggcgcgac acccatattc agcatacgcg
    11581 acaacgtcgc cggcgcatta ttggtgtgca gggtggaaaa caccatatgc cctgtttgtg
    11641 ccgccttaat cgcaatatcg gcagtttcca aatcacgaat ctcaccgacc ataatgatgt
    11701 ccgggtcctg acgcaggaaa gacttcaaag cagcggcaaa agtcaggccc tgcttatcat
    11761 tgacgttaac ctgattgatg cccggcaggt taatctcggc agggtcttcc gccgttgcaa
    11821 tatttaccga ctccgtattc aaaatattca aacaggtata gagcgacacc gtcttacccg
    11881 aacccgtcgg accggttacc agcaccatcc cgtagggacg gtgaatcgct tccaacaaca
    11941 attttttctg aaacggctca aaaccgagct ggtcgatgtt caaagacgcg gcatcggaat
    12001 tcaaaatccg catcacgacc ttttcgccaa acagcgtcgg caatgtgctg acacggaaat
    12061 cgacaggctt gccgcccttt tgaaaggtca gctgcatcct gccgtcctgc ggtatccgtt
    12121 tttcggaaat gtccaaacgc gacattacct taatccggga agcaagctgc ccccttaccg
    12181 caatgggcgg ctgaaccacc tcgcggagct gcccgtccac acggaaacgg atacgggcat
    12241 tgtgttcgta aaactcgaaa tggatgtccg atgccccgct gcgcaaggca tccgacaaag
    12301 tcttatggat aaacctcgga acagggccgt cttctgcctc ctcgttgtcg atatacaggg
    12361 tgtggctttc ctcttcctcc tgcccctccc caagctcctg aagcagcgat gtcgaacgcg
    12421 aacccaccca atcgagcaaa cccgccaact ggtcatcctc gacaatgacc aactcaacct
    12481 caatccctgc ggcagaaacg gttttctgaa tttgcggcat ctgtgtcgga tcggaaaccg
    12541 caaaaaatac tttgtcgccc cgacggaaaa ccggcacaca gtggaactcc accatctgct
    12601 cctccgtcaa cacccccatc agcaccctgt ggcgcggata atgacgcaaa tcaagaatcg
    12661 aataactgaa caccctcgca atcaatgccg caagcgactt gggcgaaatg acaccgtctg
    12721 aaaacagcat cggcaacact tccttacccg cctgcgactc attgtagtaa tgctcggcct
    12781 gctcaacagt aaccacctgg ttttgaacca gaatcctcag caaacctacg ctcatacgac
    12841 cttatcccca aatttattca ttgttatacc tgtacagctt
//
LOCUS       DAWEWP010000047        12852 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWEWP010000047 DAWEWP010000000
VERSION     DAWEWP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.047"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..383
                     /locus_tag="WGN53_000957"
     CDS             <1..383
                     /locus_tag="WGN53_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0139967.1"
                     /translation="LERLAARDIIRFPGFVNNVSEVIKEHHIFVLPSYYREGVPRSTQ
                     EAMAVGRAVITTDVPGCRETVADKVDGFLIEPWNPRILAEKMIYFIENREAVRLMGNA
                     SYAIAKDKFDAEKVDLKLLDILKA"
     gene            complement(626..1477)
                     /locus_tag="WGN53_000958"
     CDS             complement(626..1477)
                     /locus_tag="WGN53_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ0139968.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDARFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESLEIPAA
                     HKRKTAE"
     gene            1825..3339
                     /gene="katA"
                     /locus_tag="WGN53_000959"
     CDS             1825..3339
                     /gene="katA"
                     /locus_tag="WGN53_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0139969.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            3415..3552
                     /locus_tag="WGN53_000960"
     CDS             3415..3552
                     /locus_tag="WGN53_000960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139970.1"
                     /translation="MPSETSFDRFRRHFPIPPPPFQWAFFIKRKISRHSHAGGNLERK
                     I"
     gene            3872..4120
                     /locus_tag="WGN53_000961"
     CDS             3872..4120
                     /locus_tag="WGN53_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139971.1"
                     /translation="MHRTGDRVDVGWASAHQSHHSTNSANLTGAGKPMPSEGGGIGGG
                     GGLKPTLQPPHPQKNQKKPNLTKSDKKRQPETDKNCHN"
     gene            4378..4824
                     /locus_tag="WGN53_000962"
     CDS             4378..4824
                     /locus_tag="WGN53_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0139972.1"
                     /translation="MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEAL
                     TLAEGQKSAVVEYYSDNGTFPNSNTSAGIAASNEIKGKYVASVKVEGNASVASITATM
                     NSSNVNKDIKGKTLVLVGKQNSGSFSWECKKGSVDEKFLPSTCRTK"
     gene            5278..5778
                     /locus_tag="WGN53_000963"
     CDS             5278..5778
                     /locus_tag="WGN53_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0139973.1"
                     /translation="MSNYRRDFTRGAHYFFTLALQDRTKDYLIRHIDRLRLAYRETKT
                     RYPFETAAICILPDHLHFIMRLPEGDHDYPIRIAYLKTRFSQLIPKECRAPNGSKTKK
                     REAGIWQRRFWEHLIRDEQDLNNHRDYIYFNPVKHGYAASVKEWRYSSFFKDVEKGIY
                     PLDWGG"
     gene            6242..6808
                     /locus_tag="WGN53_000964"
     CDS             6242..6808
                     /locus_tag="WGN53_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ0139974.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMHEGLSGEAEDARENKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVLVAVGLTVGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIG"
     gene            complement(7084..9120)
                     /locus_tag="WGN53_000965"
     CDS             complement(7084..9120)
                     /locus_tag="WGN53_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ0139975.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(9208..10080)
                     /locus_tag="WGN53_000966"
     CDS             complement(9208..10080)
                     /locus_tag="WGN53_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0139976.1"
                     /translation="MEIWNRLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            10350..12740
                     /gene="gyrB"
                     /locus_tag="WGN53_000967"
     CDS             10350..12740
                     /gene="gyrB"
                     /locus_tag="WGN53_000967"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ0139977.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
BASE COUNT         3036 a         3427 c         3549 g         2840 t
ORIGIN      
        1 atttggaacg ccttgccgcc cgcgatatta tccgtttccc cggttttgtg aacaatgttt
       61 ccgaagtgat aaaagaacat catatattcg tattgccgtc ttattatagg gaaggcgttc
      121 cccgaagcac tcaggaggca atggccgtcg gcagggcagt gattacgacg gatgtccccg
      181 gatgcaggga aacggtcgcc gacaaggtcg acggcttcct gatcgaacct tggaatcccc
      241 gcatcttggc cgaaaaaatg atttatttta tcgaaaacag ggaagccgtc cgcctgatgg
      301 ggaatgcaag ttatgcgatt gccaaagata aattcgatgc cgaaaaagtc gatttgaaat
      361 tgctcgatat tttgaaggcg taaacaaggc tgcccgcttt tgagtttcgg tcatttctga
      421 taaacccccg tcattcccgc gaaagcggga atccaggttc gttgagtttc ggtcatttcc
      481 gataaattct tgcaactttg agtttctaga ttccctcttt cgcgggaatg acgggatgca
      541 ggtttccgcg cggacggatt cggcattccc gctttcgcag aaatgccggc ttttggtttg
      601 ttgtctttat aagattattc agcaattatt ctgcggtttt gcgtttgtgt gctgccggaa
      661 tctcaaggga ttctctgtat ttggcaatgg tgcggcgaga aacctccatg ccgcggaaag
      721 ccagcaggtt ggcgagtgcc tcgtcagaat acggcgtggg tgtggacgag gcaaaaccgg
      781 cggcgggaaa gggattgagc gaagcaatga ggtcgagtgc ggcttcgagt gtgccgctgt
      841 cggtttgggg caggtgtttt tttattcgtg cgagggtttg gctgcggttg ccgtcaatgc
      901 tgtcgagggc gtttcggacg atatgcaggg cggcgggttt ggcagcacat tcgcccgatc
      961 tttctatctg cagtatcagc gattcgttca aatcggcggc ggccacgcct gccgggtcga
     1021 attttttcaa tgcggtcagc gcgtgttgca gcattgcttc atccaacatc cactctaagg
     1081 gcgtatggtc gaggatgtct tcgatgctgt cggtcagata accctgctcg tcaaggaaat
     1141 cgataaggat gtggacacag gcggcttctt ggtcggaaag cgggtgttcg catacttgcg
     1201 cgtgcaggta ttgcttgaaa tcctgctcac cggcgatgtt ggacagcata tcttcgcctt
     1261 cgtctccgcc gatttgacgg gcaggcgcgg tgtaatggga aaacctagca tcggaaaatt
     1321 catccgtgtc tttgcgttcg agcagggggt tgtccgacag ccagttttcg acctcgcgtt
     1381 caagttcgat acccgacatc tgcaatacgc gcaaagattg ttgcagccgc tggttgagct
     1441 gctgggtctg tttgagcttt attccgagta aggtcatgat aatgtgggga aaattgttat
     1501 tttcagcctg tcggcgcaaa aaatgccgca aagcgtcatt gcattataaa tggttttaat
     1561 gagcgggttc ggattccgtt cgataacaaa aaacaaacga aaatcaagaa ccgattgctt
     1621 ataataatat taaatcgatt tcatagtttt aatagcgaaa atcttggcgt atagtcgcat
     1681 ccatagtttt tacaaaaggg aaacaaaatg tcgattcaag aaatttattg caatcaagaa
     1741 accggttacg aatacgcttt ccgccaaatc gtactgtaag ccgtttaccc ccatttgacc
     1801 aacctgagaa aaggaacaag agcgatgact acctccaaat gccccgtaac ccatctgacc
     1861 atgaacaacg gcgcgcctgt tgccgacaat caaaacagcc tgaccgccgg tcctcgcggc
     1921 cctctgctgg cgcaggattt gtggctgaat gaaaaactcg ccgacttcgt gcgcgaagtc
     1981 atccccgaac gccgtatgca cgccaaaggt tcgggcgcgt tcggtacgtt taccgtaacg
     2041 cacgacatca ccaaatacac ccgcgccaaa atcttcagcg aagtcggcaa aaaaaccgag
     2101 atgttcgccc gtttcaccac cgtggcaggc gaacgcggcg cagccgatgc ggaacgcgac
     2161 atccgcggct ttgccttgaa gttctatacc gaagaaggca actgggatgt ggtcggcaac
     2221 aacacgcccg tgttcttcct gcgcgacccg cgtaagttcc ccgacctgaa caaagccgtc
     2281 aaacgcgacc cgcgcaccaa tatgcgctct gccacaaaca actgggactt ctggacgctg
     2341 ctgcccgaag cactgcacca agttaccatc gtgatgagcg accgcggcat ccccgccggc
     2401 taccgccata tgcacggctt cggttcgcat acctacagct tctggaacga agcaggcgag
     2461 cgtttttggg tgaaattcca tttccgcacc caacaaggca ttaaaaacct gaccaacgaa
     2521 gaagccgcca aaatcatcgc cgacgaccgc gaaagccatc agcgcgactt atacgaagcc
     2581 atcgaacgcg gcgagtttcc gaaatggacg atgtacatcc aagtcatgcc tgaagcagac
     2641 gcggaaaaag taccttatca tccgtttgac ttgaccaaag tttggccgaa aaaagactat
     2701 ccgctgattg aagtgggcga attcgagttg aaccgcaatc ccgaaaactt cttcgccgat
     2761 gtggaacaat ccgccttcgc accgagcaac ctcgttcccg gtatcggcgc gagcccagat
     2821 aaaatgttgc aagcccgttt gttcaattac gccgacgcgc aacgctaccg cttgggcgtg
     2881 aacttccgcc aaatccctgt caaccgtccg cgttgccctg ttcacagcaa ccagcgcgac
     2941 ggacaaggac gcgccgacgg caactacggc agcctgccgc actacgaacc caatagcttc
     3001 ggccaatggc agcaacaacc cgacttcgcc gaaccgcctt tgaaaatcaa cggcgacgcg
     3061 gcgcactggg actaccgcca agacgatgac gactatttca gccaaccgcg cgccctgttc
     3121 aacctgatga acgacgcgca gaaacaggca ttgttcggca acaccgccgc cgcaatgggc
     3181 gacgcgcccg acttcatcaa ataccgccat atccgcaact gctaccgttg cgatccggca
     3241 tacggcgaag gcgtggcaaa agccctcgga ctgactgtcg aagacgcaca agccgcccgc
     3301 gcgaccgacc ccgcactcgg ccagcccggt ttgctgtaag ggggcattat gtggatggaa
     3361 attgaagaaa tcctgtcccg cgccgtccgc taccggaaat aaccgggcat aaaaatgccg
     3421 tctgaaacgt cgtttgaccg cttcagacgg catttcccca tcccgccccc gccgtttcag
     3481 tgggcgtttt ttattaaacg caaaatatcc cgtcattccc acgcaggcgg gaatctagaa
     3541 cgtaaaatct aaagaaacct ttttccccat aagtttccgc accgacagac ctggattccc
     3601 gcctgcgcgg gaatgacggt ttaggtattt ctgacgattc agtaagccgt atcggactgt
     3661 agaatgggct gtaaggtggg cgtagggtgg gcttcagccc accaatccca ccaacccact
     3721 tttccgcgtc attcccacga aagtgggaat ctagaacgta aaatctaaag aaaccttttt
     3781 ccccgataag tttccgtgcc gacaggtctg gattcccacc tgcgcgggaa tgacggttta
     3841 ggtattcaca taaggcggat gtgtaaggta gatgcatagg acgggcgata gggtggacgt
     3901 agggtgggct tcagcccacc aatcccacca ttccaccaac tccgccaatc ttaccggtgc
     3961 cggcaaaccg atgccgtctg aaggcggcgg aatcggcgga ggcggtgggc tgaagcccac
     4021 cctacagcca ccccaccccc aaaaaaacca aaaaaaaccc aatctgacaa aatctgacaa
     4081 aaaacgtcag cccgaaactg acaaaaattg tcacaactga caagaaacgt cagcccgccg
     4141 cattattacg ccgcaagccg cgccgcgttg atataaaaaa atacccatac cagacagaca
     4201 aataaaaaaa tattctgtaa aatgccgagc tgaaccctgt tttggcacgc cgcttgctgg
     4261 taagaaggcg aaccgaagta aaaaaaacca cggtcaaata cattacgggt ttacgtttgc
     4321 ggcggatgcc gcaaatttcc aatcaaactt aaacattttc aaacaggagt catccaaatg
     4381 aaagcaatcc aaaaaggttt caccctgatc gagctgatga tcgtcatcgc catcgtcggt
     4441 atcttggcag ccgtcgccct gcccgcatac caagactaca ccgcgcgcgc ccaaatgtcc
     4501 gaagccctga ctttggcaga aggtcaaaaa tccgcagtgg tcgagtatta ttccgacaac
     4561 ggcacattcc cgaacagcaa tacttccgca ggtattgctg cctctaacga gattaaaggt
     4621 aagtatgtgg catcggttaa ggttgaaggt aatgcctctg ttgcttctat taccgctacc
     4681 atgaactcta gtaatgtgaa taaggacatc aaaggtaaaa ccttggtact cgtcggcaaa
     4741 caaaactccg gctcgttctc ttgggaatgt aaaaaaggtt ctgtggacga aaaattcttg
     4801 ccatctacct gccgcaccaa ataaggacaa tgaccgggtt tgacccggtc gtgaaaacaa
     4861 atgccgcccg aaccgctcgg gcggcatttt ctattcccct ttgccgctgt agggctgtag
     4921 ggtgaggcgt aggtgcgtag ggtgggctgt agggtgggct tcagcccacc attccaccaa
     4981 cccaaccaac tccaccacat caaggatttt tggcgaaccg ttgggattcg gcgggtttcg
     5041 agtttcggcg gggattggcg gaatggttgg tttgggttgg gtgggaatgg ttggattggc
     5101 ggaatggtgg gctgaagccc accctacgaa acaccctacg aaacacccta cagcctacac
     5161 atcagcccta cgccctccct acccatttcc ctgcaaatcc cctaaaaatc ctcccgcacg
     5221 ccctgaaaac aaggaataat acaggtttcc gcccatcggg ctaaaaaggg gataaatatg
     5281 tcaaattacc ggagggactt cacgcgcggg gcgcactatt ttttcacatt ggctttgcaa
     5341 gacaggacga aggattacct gatacggcat atcgacaggc tgcgcctcgc ctatcgtgaa
     5401 acgaaaacac gttatccgtt tgaaacggcg gcaatttgta ttttgccgga tcacctgcat
     5461 ttcattatgc ggctgccgga aggcgaccac gattacccga tacggattgc atatttgaaa
     5521 acccggtttt cccaactcat ccccaaagag tgccgcgccc ccaacgggag taaaacaaaa
     5581 aaacgcgaag ccggcatttg gcagcgcagg ttttgggaac acctgatccg ggacgaacaa
     5641 gatttaaaca accaccggga ttatatttat ttcaaccccg tgaaacacgg gtatgcggcg
     5701 agtgtgaagg aatggcgtta ttcttccttc ttcaaagacg ttgaaaaagg catttaccct
     5761 ttggattggg gagggtaaag ctcccgccgc cgccaaaatc cgcaggacgg acgcggggca
     5821 gatgagtcgg gcgggacgta gggtgaggcg taggtgcgta gggtggatgt gtagggtggg
     5881 cttcagccca ccaatccaac cacctccgcc aatccaacca ttcccaccaa cccaaaccaa
     5941 ccattccgcc aatccccgcc aaaaaccgaa accgccgaat cccgacggtt tgccaaaaac
     6001 ccttgatgcg gtggaattgg cgggattgga tgagttggtt tggcagaatt ggtgggctga
     6061 agcccaccct acagcccacc ctacaacccg ccctaagtcc cacctacgtt ccatccgacg
     6121 catccgccct acggttccgg attcccctgc gaaccgcccc gcgcacgaaa actgctttac
     6181 gcggcgtttt tcgcgttttg ctgttttgct ataatccgcc ctttttaagg acgggtgcgg
     6241 tatgagtctt tacgctttgc tcttgattgc gctggggatg tcgatggatg cgtttgccgt
     6301 cgcgctggca aagggtgcgg cggtcaggat gcctccgcgc aaaattgcag caacagcctt
     6361 ggtgttcggc acggttgaag cgctcacgcc gctggcaggc tgggtaggcg gtttttatgc
     6421 caagccgttt atcagcgaat gggaccattg ggcggctttc gtcctgctgg gcgggctggg
     6481 tctgaaaatg atgcacgaag ggttgtccgg cgaggcggag gacgcgcggg aaaacaaacg
     6541 ggaaagctgg tggttgacgg ttctgactgc ttttggaacc agtattgatt ccatgatagt
     6601 cggggtgggc ttggcgttta tggaggtaaa catcgccttt gccgccgcaa tcatcggtat
     6661 ggcgacgacg gtgctggtgg cggtcgggct gacggtggga agggctttgg gcgtattgtt
     6721 cggcaggcgt gcggaatttg ccggaggttt ggtgttgatt gccatcggta catggacgct
     6781 cttgtcgcac ttggggctga tcgggtaagg actgccaaaa aatgctgtct gaagcgttgt
     6841 ttgaccgttt cagacggcat tttttatttt ttgggcgtag ggcgaatccg taaggtggga
     6901 tgtaaggtgg gatgtagggt gggcttcagc ccaccctacg cccactgttt ttgatttttt
     6961 gtttttgcgg gaatgacggg attttggctt actgtttttg tgaaaatggc gaaaccgcgc
     7021 aaatgctgat gccgtctgaa acactgtttg attgtttcag acggcatttt ttgttcacat
     7081 cgatcaagcc gcgttgtcga aaccgctgtg gcgcaagagt gcgtctatgc tcggttcgcg
     7141 gccgcggaag gctttgaagg attctgccgc gctgcgcgat ccgccgacgg cgaggatttc
     7201 ctgccaaaag cgtttgcctg tggcggcgac atcgtcgctt tcttcaaagg cggcgtatgc
     7261 gtccgcgctc aatacttccg cccacgcgta gctgtaatag cctgcggaat agccgcctgc
     7321 gaagatgtgg ccgaagctca aggcgaagcg gttgtattcg ggcggctgga tgacggcgac
     7381 ttttttgcgc acgctgtcta aaacctgttg ccagtttttc agacggcctt cgtcgtcttc
     7441 gctgtaaatc atcatatcaa agagggcgaa ctccatttgc cggacgagga acatgccgcg
     7501 ttggaagttt ttggcggcga gcattttgtc gaagagttct ttcggcaggg gaacgccggt
     7561 ttcttcgtgg gctgacattt gtgccaagac attgtattcc caaacgaaat tttccataaa
     7621 ctggctgggc agttcgaccg cgtcccattc tacgccgttg atgccggata cgcccagttc
     7681 gtccacttgg gtaagcaggt ggtgcagccc gtgtccggtt tcgtggaaga ggatgaggat
     7741 ttcgtcgtgg ctcaggcggg cttccctgcc gccgacgggt ggggcgaagt tgcagacgag
     7801 gtaggcggtg ggcagttgca gcgtgccgtc tgaaaaacgg cggcggcctt tgtagtcgtt
     7861 catccacgcg ccgccgcgct tgccttcgcg ggcgtagagg tccatataga cgccgcctat
     7921 ggttttgccg ttttgttgca attcaaaata gcgcacgtct ttgtgccaga cggggacggt
     7981 tttttcggta aatccgatgc cgtagagttt tttgatttgg gcgaacagtc cgtttaatac
     8041 tttgccgacg gggaagtatt ttttgacttc ggtttcgctg aacgcgtatt tggcttcgcg
     8101 cagtttttcg ctggcgtagc ccaagtccca cggttgcaaa tcggcgaggc cgaggctttc
     8161 gcgggcgaag gctttgactt cggcgaggtc tttttcggcg tagggtttgg cgcggcgggc
     8221 gaggtcgtgc aggaagttta aaacttgttc gggggtgtcc gccattttgg ttgccagcga
     8281 caattcggcg tagtttttga agccgagcag tttggcggtt tgcagggcgt ttgcgagcgt
     8341 gcggtcgatg ttggcggtgt tgtcgaattt gccgtcgtct gacagttcgc tggcgcgggt
     8401 aacgtaggcg cggtagattt gttcgcgcag ttcgcggttg tcggcgtatt ggatgacggc
     8461 gaggtagtgt ggaatctgca agccgatttt gtagcctgtt ttgctttcgc tttgcgcggc
     8521 ggcggcaaac atggcgagcg cgtcttcggg aatgccggca agcggtgcgg catcgtcaaa
     8581 gtaaatgccg aacgcgtcgg tcgcgtctag gacgttttgg gagaatttgg cggaaagttg
     8641 cgcgccttcg gtttgcagtt ttgccagttc tgcctgctgt tcgggcggca gttccgcgcc
     8701 gctgaggacg aaatcgcgca gatcgtggtt gagtttggtt ttttgtgcgg gggagagggt
     8761 gtcgaattcg ggggaatttt tgatggtttt gaagcggttg tacagctcga tgtcttgtcc
     8821 gatttcggtg aagaagacgg tgatttcggg catcagttcg ttatagacgg cgcgcagttc
     8881 gggcgtgtcg gcgacggagt tgaggtgcga caccacgccc caaatcctgc cgacgcgttc
     8941 ggtgatgccg gtcaggggtt cgacagtgtt tgcccagccg gtgtgcgttt gggctttgat
     9001 ggcggcgatt tgttcgcgcg cttcggcgat ggcggtttgc agggcgggtt tgatgtcttc
     9061 ggttttgatt tgatcaaaac ggggttcttc gcccaaatgg agcagtgcgt tgtcagtcat
     9121 aagatgggtt tcctttgcgt gttgtgcttc agacagcata tcggcaatat gccgtctgaa
     9181 gccggaaatg gggtcggtcg gggaaagtta aagtgtttcg gagccggcgg ggtaatttaa
     9241 caggcggtat tgtacgcgca aaaattcgtc cataaccgcc tgttggattt ccagccgctt
     9301 tgaaacgggg gaggcgaagc ggacgatgat gcggtatgcc ttgtcatcgt acggcacgcg
     9361 ggtaacgcgc ggtctggcgg cgggcgtgat aaacagtttt tccgcctgca cgttttccaa
     9421 atgccgttgg atggcgggga tgtagggcgc gcacaagggc tcgagtacgg ctttcagacg
     9481 gcatacggct tcatccgaat ccaaatggat ggggacgggg atttcgaccg tatggatgac
     9541 atagtcgccc aaaatattgt cgcggcgcac ggggtggctc aacaacaggc tgttggggaa
     9601 agaaacggtg gttcccgcaa gctgtccgac caaggggttc ggaccgacct gcatcatcag
     9661 cgtgttcaac aggttgatgt cgaccacgcg cccgcgcagg ccgttgattt cgatatagtc
     9721 gccgaccgag tattgctggg tggcagacct taaaatactg cccgacagac acataatcag
     9781 ttccttcgtc gccacgacga ccgccgccgc caccgcaaac atcgacaaag ccagcgtttg
     9841 gatttgcgcc gaccagataa atgccagcga aaacagcacc aaaagcagcg ttatattgcg
     9901 gctggcaacc aaaaaccgcc gcttgctttc gatgccgaaa tccggatgcc gtttgaagtg
     9961 gatattcaac agaagggcgc gcgccagcag caaagccgca accgccgcca cggattcgac
    10021 cgcctccgca cgtatcggga cggcaccgag ccaagtgtcc aacctattcc atatttccat
    10081 ttccgcgccc ctgtccgaaa agtatagggt gtagatttta gtggcaaaaa aacgcttttg
    10141 ccactttacg ggaaaatccc cgacctgatg ccggaggcaa acgtaccgtt tcatcttttc
    10201 ccggcggcac actgatgcgg ataacgctga attaggcggc aaaatcggct ataatacaca
    10261 attatatatg ccgtcccgcc cgctttttcc acaaggtgcg gacttgtttt ggcggcatcg
    10321 caaaatcttt tcaaaatccg gcaaaaaata tgactgaaca aaaacacgaa gaatacggcg
    10381 ccgacagcat tcaggtgctc gaaggcttgg aagcggtacg caaacgcccc ggcatgtaca
    10441 tcggcgacac gcaggacggc agcggcctgc accatatggt gttcgaagta ttggacaacg
    10501 ccattgacga agcactcgcc ggacattgcg acaaaatcac ggtaacgata cacgccgacc
    10561 attccgtcag cgtcgccgac aacgggcgcg gtatgcccac cggcatccac ccgaaagaag
    10621 gacgctccgc cgccgaagtc atcatgaccg tattgcacgc gggcggtaaa ttcgacaaca
    10681 acagctacaa aatctccggc ggcctgcacg gcgtgggcgt gtccgtcgtc aacgcgctgt
    10741 ccgactgggt aacgctgacc atctaccgcg acggcaaaga acacttcgtc cgcttcgtgc
    10801 gcggcgaaac cgaagagccg ctgaacgttg tcggcgattc cgataaaaaa ggcacgaccg
    10861 tgcgcttcct cgccagtacg gaaaccttcg gcaacgtcga atacagcttc gacatccttg
    10921 ccaaacgcat ccgcgaactt tcgttcctga acaacggcgt ggacatcgaa ttgaccgacg
    10981 agcgcgacgg caaacacgaa agcttcgccc tttccggcgg cgtggctgga ttcgtgcaat
    11041 acatgaaccg caaaaaaacg cccttgcacg aaaaaatctt ctacgcgttc ggcgagaaag
    11101 acggcatgag cgtcgaatgc gcaatgcaat ggaacgacag ctatcaggaa agcgtgcagt
    11161 gcttcaccaa caacatccct cagcgcgacg gcggtacgca cctgaccgcg ttgcgccaag
    11221 tgatgacgcg caccatcaac agctacatcg aagccaacga agtcgccaaa aaagccaaag
    11281 tggaaaccgc cggcgacgat atgcgcgaag gtttgacctg cgtgttgtcc gtcaaactgc
    11341 ccgaccccaa attttcgtcc caaaccaaag acaaactggt ttccggcgaa atcggtcccg
    11401 tcgtcaacga agtcatcagc caagccctga ccgacttcct cgaagaaaac cctgccgaag
    11461 ccaaaatcat caccggcaaa atcgtcgatg ccgcccgcgc gcgcgaagcc gcccgcaaag
    11521 cccgcgaaat cacccgccgc aaaggcgtaa tggacggctt ggggctaccc ggcaaactcg
    11581 ccgactgcca agaaaaagac cccgcattgt cagaacttta tttggtcgag ggcgattctg
    11641 caggcggttc cgccatgcag gggcgcgacc gcaaattcca agcgattttg ccgcttaagg
    11701 gcaagatttt gaacgtggaa aaggcgcgct ttgaaaaaat gctcgccagt caggaagtcg
    11761 ccgctttgat tactgcgttg ggtgcaggta tcggcaagga agaattcaat gccgagaagc
    11821 tgcgttacca ccgcatcatc attatgaccg atgccgatgt ggacggcgcg cacatccgca
    11881 ccctgctttt aaccttcttc taccgccaaa tgcccgagct ggtcgaacgc ggctacatct
    11941 acattgccca gccgccttta tacaaagcca aatacggcaa acaagaacga tatctcaaag
    12001 acgaattgga aaaagaccaa tggctgctcg gtttagcctt ggaaaaagcc aaaatcgttt
    12061 cagacagccg caccatcgaa ggcgaagagc ttgccaatac cgccaaacaa ttcctgttgg
    12121 ccaaaaccgt catcgaacag gaaagccgct tcgtagacga actcgtcctg cgcgccatgc
    12181 tacacgcgtc gcccattgat ttgacgtcgt ctgaaaacgc cgataaagcc gttgccgaac
    12241 tttccggtct gcttgacgaa aaagaagtcg ccctcgaacg catcgaaggt catgaaggac
    12301 accgattcat caaaatcacg cgcaagctgc acggcaacgt catggtcagc tacatcgaac
    12361 ccaagttcct caacagcaaa gcctaccaaa ccctcaccca aaccgccgcc gcgctcaaag
    12421 gcatggtcgg cgagggtgcc aagctttaca aaggcgaaaa cgggtacgac gcggacagct
    12481 ttgaaaccgc tttggacatc ttgatgagcg ttgcccaaaa aggtatgtcc atccaacgat
    12541 acaaaggctt gggcgagatg aaccccgaac agctgtggga aaccacgatg gatcccgccg
    12601 tgcgccgcct gttgaaagtg cgcatcgaag acgccatcgc cgccgacgaa gtgttcgtta
    12661 cgctgatggg cgacgaggtc gagccgcgcc gtgcctttat cgaaaacaac gcgctgattg
    12721 cccaaaatat cgacgcataa gtgccgtttt aaaaaaggag acgggcatcg tgccgcgtct
    12781 ccttttggtt tgtcaaacgg aagccatgcc gtctgaaacc gcctttcgga gcaaaaccat
    12841 gatcagcatt tt
//
LOCUS       DAWEWP010000048        12748 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWEWP010000048 DAWEWP010000000
VERSION     DAWEWP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.048"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(142..232)
                     /locus_tag="WGN53_000968"
     tRNA            complement(142..232)
                     /locus_tag="WGN53_000968"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Ser,seq:gga)
     gene            complement(268..354)
                     /locus_tag="WGN53_000969"
     tRNA            complement(268..354)
                     /locus_tag="WGN53_000969"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(318..320),aa:Leu,seq:cag)
     gene            complement(390..476)
                     /locus_tag="WGN53_000970"
     tRNA            complement(390..476)
                     /locus_tag="WGN53_000970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Leu,seq:cag)
     gene            565..2940
                     /gene="rnr"
                     /locus_tag="WGN53_000971"
     CDS             565..2940
                     /gene="rnr"
                     /locus_tag="WGN53_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ0139978.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQF
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAIKVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAGKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3279..4742)
                     /gene="guaB"
                     /locus_tag="WGN53_000972"
     CDS             complement(3279..4742)
                     /gene="guaB"
                     /locus_tag="WGN53_000972"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ0139979.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSADKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4889..7447)
                     /gene="glnD"
                     /locus_tag="WGN53_000973"
     CDS             complement(4889..7447)
                     /gene="glnD"
                     /locus_tag="WGN53_000973"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ0139980.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKTNRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGGIDELCESVRNDITGDTAFLEARFLFGNRQTADELAEKMNAQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATNLPALVK
                     QGILTRTEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYQGLNLRR
                     QSEELMRVFYRAIKTVKQLSGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFERQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSTVAQKEDIQDPSVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLAGNGGNPHALFGRRQQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEIFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            complement(7496..7816)
                     /locus_tag="WGN53_000974"
     CDS             complement(7496..7816)
                     /locus_tag="WGN53_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0139981.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            complement(8140..8613)
                     /gene="bfr"
                     /locus_tag="WGN53_000975"
     CDS             complement(8140..8613)
                     /gene="bfr"
                     /locus_tag="WGN53_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0139982.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLEKEKEKHEALI
                     AAISVAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(8641..9105)
                     /gene="bfr"
                     /locus_tag="WGN53_000976"
     CDS             complement(8641..9105)
                     /gene="bfr"
                     /locus_tag="WGN53_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0139983.1"
                     /translation="MQGNQAVLDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(9568..10551)
                     /gene="lipA"
                     /locus_tag="WGN53_000977"
     CDS             complement(9568..10551)
                     /gene="lipA"
                     /locus_tag="WGN53_000977"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ0139984.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(10544..11167)
                     /gene="lipB"
                     /locus_tag="WGN53_000978"
     CDS             complement(10544..11167)
                     /gene="lipB"
                     /locus_tag="WGN53_000978"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ0139985.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNANRTASTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPIVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKGGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11169..11444)
                     /locus_tag="WGN53_000979"
     CDS             complement(11169..11444)
                     /locus_tag="WGN53_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ0139986.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHKLVKVVL"
     gene            11629..12699
                     /locus_tag="WGN53_000980"
     CDS             11629..12699
                     /locus_tag="WGN53_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ0139987.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
BASE COUNT         2717 a         3014 c         3729 g         3288 t
ORIGIN      
        1 taccgcaaaa cataccacaa aaaacggtaa cggatagttg taagcggttg atgacgatta
       61 tagacaataa cgggttttcc caatgaaatt attgtttgaa taataaaaaa tcccaaaccg
      121 taaaagtttg ggatttgtat ttggcagaga ggaagggatt cgaaccctcg atacgctatt
      181 cacgtataca cgctttccag gcgtgcgact taaaccactc atccacctct ctaatggcgg
      241 aaattatccc aatcggaata atttatttgg tgcccgggag aagactcgaa cttccacacc
      301 cgtgaggata ccagcacctg aagctggcgc gtctaccaat tccgccaccc gggcaatcta
      361 aatattgaaa taaagcaaag catttgattt ggtgcccggg agaagactcg aacttccaca
      421 cccgtgagga tactagcacc tgaagctagc gcgtctacca attccgccac ccgggctttg
      481 ctttatttgc tgttttgcgg tacagtgtcc tgccgcaaag aagtcgctat tatatagttc
      541 ataaagagaa tgtcaacagt ccaaatgaat aaaaatatta aatctttaaa tttacgggaa
      601 aaagacccgt ttttaagtcg tgaaaaacag cgttatgaac atcctttgcc cagtcgggaa
      661 tggataatcg aattgttgga gcgcaaaggt gtgccttcaa aaatcgaatc gcttgcgcgc
      721 gagctgtcga ttacggaaga cgagtatgtc tttttcgaac gccgtctgaa ggcgatggcg
      781 cgggacggtc aggttttaat caaccgccgg ggcgcggttt gcgcggcgga caaattggat
      841 ttggtcaaat gccgtgtcga ggcgcacaaa gacggtttcg gttttgccgt gccgctcacg
      901 cccgccaaag acggtgattt tgttttgtat gaacgccaga tgcgcggcat tatgcacggc
      961 gatattgtca ctgttcgtcc tgccggcatg gaccgtaggg gccgccgcga agggacagtt
     1021 ctggatattg tcgaacgcgc gcaaagcaaa gtggtcggcc gtttctatat ggataggggc
     1081 gtggcgattt tggagccgga agacaagcgt ctgaaccaaa gcatcgtatt ggaaccggac
     1141 ggcgtggcgc gtttcaaacc tgaatccggt caggtcatcg tcggcgaaat tgaggtttat
     1201 cctgagcaaa accggccggc agtggcaaaa atcatcgaag ttttgggcga ttatgccgac
     1261 agcggcatgg agattgaaat tgccgtgcgc aagcatcatt tgccgcacca attcagtgaa
     1321 gcgtgtgcca aagccgtgaa aaaaattccc gaccatgtac gcaaaagcga tttgaaaggc
     1381 cgcgtcgatt tgcgcgacct gcctttggta acgattgacg gcgaaacggc tcgagatttt
     1441 gacgatgcgg tgtttgccga aaaaatcgga cgcaattacc gtctggtcgt ggcgattgcc
     1501 gatgtcagcc attatgtccg ccccgatgac gctatcgaca cggacgctca ggaacgcagc
     1561 accagtgttt acttcccgcg ccgcgtgatt cccatgttgc cggaaaacct gtccaacggc
     1621 atctgctcgc tcaatcctaa tgtcgagcgt ttgtgtatgg tgtgcgatat ggttatcact
     1681 tacgcgggca atattaaaga ataccgcttc taccccgccg tgatgcgctc tcatgcccgc
     1741 ctgacctaca accaagtttg gaaatggctt tcaggcggca tcgggcatcc gttcaaagcc
     1801 caaatcgaca cgctttacaa actcttcaaa atccttcaga aaaagcgttt cgaacgcggg
     1861 gcggtggagt ttgacagcat cgaaacccaa atgcttttcg acgacaacgg taaaattgaa
     1921 aaaatcgtcc ccgttgtccg caacgatgcc cacaagctga ttgaagaatg tatgttggcg
     1981 gcaaacgttt gcgcagcgga ttttctgttg aaaaacaagc ataccgcatt gttccgcaac
     2041 catttggggc ccacgcccga aaaactcgcc gccttgcgcg agcagttcgg tctgttgggg
     2101 cttcaacttg gcggcggcga caacccgtcg ccgaaagact atgccgcgct tgccggacag
     2161 ttcaaaggca ggccggatgc cgaattgctg caagtcatga tgttgcgctc catgcaacag
     2221 gcggtttacg aaccgcattg cgacggacac tttggtcttg cctacgaagc atacgcccac
     2281 ttcacctcgc ccatccgccg ctatcccgac ctgaccgtac accgcgccat caaagccgtg
     2341 ttgaatcagc aaacctacac gccaaaaaaa agctggcagg ctttgggcgt gcatacctcg
     2401 ttctgtgagc gccgtgccga cgacgccagc cgcgacgtgg aaaactggct gaaaacctac
     2461 tatatgcgtg acaaagtcgg cgaaatattt gaaggcaaaa tttccggggt ggcaaatttt
     2521 ggaatatttg tcactttgga cgatatccat atcgacggtc tggtgcatat cagcgatttg
     2581 ggcgaagact atttcaactt ccgccccgaa atcatggcaa tcgaaggtga acgcagtggc
     2641 atccgtttca atatgggcga cagggttgcc attaaagtgg cccgtgccga tttggatgat
     2701 ggaaaaatcg attttgtcct gattgccgag gggagcggca gagggcggaa agttaaatca
     2761 tccgcgtctg ccaaaccggc agggacggcg gggaaaggga agccgaaaac cgccgccggg
     2821 aaaaaaacag cccgaggcgg caaagtaagg ggaaggggcg cgtctgccgc cgcagaatcg
     2881 gggaaaaagg caaagaaacc ggttccgatt aaggtaaaaa aacggaaagg caaatcataa
     2941 tgctgacggg gcggcttggg gagggagggg catactttcc cgaagccgcc gcccgatttt
     3001 ttgatatggc ggccggcgca tcatttcagg ccaatcgaaa cgcccgtatt gaaagattgc
     3061 gtttatttcc accgccgttt taaaggccag cggtattcgg cagacggggc gcaaacggcg
     3121 ttcagacggc attttcattc tttcggcgtg tccgtccgaa tggctttgcc cgtccgcgca
     3181 atcagcccgt gcggcttgcc ttgaacggac aaaaaatgcc gtctgaaacc cgaaaatcag
     3241 gtttcagacg gcattttgcc ttgaaaaggc tgttcaaatc agcggtggta gttcggtgct
     3301 tctttagtga tttgaacgtc gtgaacgtgc gattcgctca tacctgcgga agtgatttcc
     3361 acaaattcgg ctttttcgtg catttcggca atattggcgc aacccaagta acccatactg
     3421 gaacgcagac cgccggtcag ctggtggatg atgttcacaa tcgggccttt gtaaggaacg
     3481 cggccttcga tgccttcggg gacgtatttg tctgcgctgt cggttttgtc ttggaagtag
     3541 cggtcggcag aaccttggct catcgcgccc aaggaaccca taccgcgata ggatttgtac
     3601 gagcggcctt ggtagagttc gatttcgccc ggcgcttctt ccgtgcctgc aaacataccg
     3661 ccgagcatga cgctgtacgc gcctgcggcg agggctttgg cgatgtcgcc ggagaagcgg
     3721 atgccgccgt cggcaatcag cggaacgccc gtgcctttga gggcttcggc aacgttgtga
     3781 atggcggtca gttgcggcac gccgacacct gccacgatac gggtggtgca aatcgatccc
     3841 ggaccgatac cgactttgac ggcatccgcg ccggcggcga ccaaatccaa tgcggctttg
     3901 gcagtggcga tgttgccgcc gatgacttgg atgtgtggat aggtttcttt cacccatttc
     3961 acgcggtcaa tcacgccttg gctgtgcccg tgggcagtat cgacgacaat cacgtccacg
     4021 ccggcttcga ccaaggcttt gacgcgctct tcggtatcgc cgccggtgcc gactgccgca
     4081 ccgacgcgca gacggccctc ggagtctttg ttggcgttgg gaaactcggt ggtttttaaa
     4141 atatctttaa cggtaatcag acctttgagt tcgtcttttt cgttcagaac caaaacgcgc
     4201 tcgactttgt gcgtgtgcat cagttcgcgc gcttcgtcta tgcttgtgcc ttcggggacg
     4261 gtaaccagac gttcgcgcgg ggtcataatg gcggaaacgg gcaaatcgac gcggttttca
     4321 aaacgcaggt cgcggttggt tacgatgccg acgactttgc cgttttcaac gacgggcagg
     4381 ccggacattt tgcgtttgcg ctgcgcgcgc atttccaaga cttcgcggat gagcgtcgtc
     4441 ggtgcaacgg ttacggggtc tttgaccacg ccgctttcgt ggcgtttcac tttggaaacg
     4501 gcgcgcgcct gcatttcggg cggcatgttt ttatggatga tgccgatgcc gccttcctgt
     4561 gccatcgaaa tggcgaggcg cgcctcggta acagtgtcca tcgcggcgga aagcaggggg
     4621 aggttaagtg tgatttcgcg ggtgagcttg gtttgaagtt taacgtctcg cggcagcacg
     4681 gtcgaatgtg cgggaaccaa caaaacatcg tcgaaagtat aggctttttc tacgatacgc
     4741 ataatgctcg gtctttcagt ttgtgcaaga tgcacggcat tatagcacgt taccggcggc
     4801 ttgacagtct atattgtttg caatatcccc cgccttcaga cggcatacgg cgcggcggcg
     4861 gaatgccgtc tgaaaaccgg cagggtgctt atgccaactg ttccagcaaa gcctgtttca
     4921 atgaggactg gatttttggg tttttcaggt cggggctaaa aacggtaaaa ctgtcttcgg
     4981 cgcgttcatc cagtgtggag attttggcat agcgcaggct gacgttgtgg gcgaaaaaga
     5041 tttccgccat atcggcgagc aggaaggggc ggttgacggc ggtaatttcg acggagtacc
     5101 agtcggggta gtcttcttcg ggggtaatgg tgatgcttgg cgcgatcggc atgtagcggc
     5161 tgcggcggct gatgcggcgg ctatggctgg agatttcggc aacggtgtgt ccgtggataa
     5221 agctgttgag ttcggcttcg agcgcgcttt ggatgtcggg gtagtcttcg ggagcgtgct
     5281 gcgaggggat ttgcacgatg aaggtgtcga ggatgtagtc gtgttcggtg ataaaggcgc
     5341 gggcggcgag aatgtcgaag ccgtggcggc tgaagatacg gcagaggcgg gcgaacaggc
     5401 gcgggccgtt gggcataaag accatcactt ggaagctgtc gctttggggc aggatgcggc
     5461 tgcggacgat gggggtttca aaatcgtgta cgaggttggc ggcgtgccac aggatttcgc
     5521 gggactggtg gcgggcgaag taggcggaac cgagcgcgtt ccaaagcttt ttctgctgtt
     5581 tttcggggac ggcggcgcgg gtgagcaagt cggcggcttc ttgttggcgg cggccgaaga
     5641 gggcgtgcgg gttgccaccg ttgcctgcga ggtagcgtcc ggcggcatgg aagaggcttt
     5701 ccagcaggct ggcgcgccat gcgttccaca gcttgggatt ggtaccgcgt atgtcggaaa
     5761 tggtcagaag gtagagcgcg ctgaggcgtt cgtgggtttg gacgcgtttg cagaaggcat
     5821 cgagtacgct ggggtcttgg atgtcttctt tttgggcgac ggtggacata agaagatggt
     5881 tttcaaccag ccaggcgagc aggtcgcttt cttcttcggt caggaagtgg tcggcggcaa
     5941 attggcgcgc gtctgcgatg ccttgtatgg catggtcgcc gccgcgtcct ttggcgatgt
     6001 catggaaaaa ggcggcaagg tagaggatgt cttgtctttc aaaggactgc atcagtgcag
     6061 aggcgtaggg cagctcgtgg ctgtgcatat ccaaggcaag gcggcggacg ttgcggacga
     6121 cggtgaggat gtggtcgtcc acgggataga tgtggaacag gtcgtgctgg agcaggccga
     6181 cgattttttc ccacgcgggc aggtagcgtc ccaatacgcc gtagaggttg aggaagcgca
     6241 gggtttgggt cagtccgttg ccgttgcgga aaaagccggc gaagcggtgg cggttttcag
     6301 aattttggta gaagctgcgg ttgattttgc gcgtcgcgcc ccaccatgcg cgcagggttt
     6361 gcggttcgag cgcggtaata tcgttgcgct gctgcatgat ttcgacgatt ttgaaaatgt
     6421 gttcgggccg tctgaaaaaa atatcggtgt gccgcgcggc gatttggttg ttgacttgga
     6481 tgtagtcgtc gtcaatccgc agggtaacgc gcaacggggt ggaggaaacg cggctttgca
     6541 gcataggcgt gaggatgccg ctcagttgtt tgacggtttt aatcgcgcgg taaaacacgc
     6601 gcatcagttc ttcgctttga cggcggaggt tcaagccttg ataacccata ctttcggcga
     6661 cttgcggctg caaatcgaac agcaggcggt cttcggcgcg cttggcgttt aaatgcagat
     6721 ggatgcggat gtgggcgagg cggcggtagc cgtgtgaaag cataccggct tcagtgcgcg
     6781 tcagaatgcc ttgtttgacc agcgcaggca ggttggtcgc caagccttgt gctttcgcta
     6841 tccaaagcag ggtatggatg tcgcgtagac cgccgggaca gcttttgatg tttggttcca
     6901 acaccgctcc cgaaccttgc gatttggcat ggcggtgttc catctccacc aattttgctt
     6961 cgataaatgc cgctacattg cgttgcgcgt tcattttttc cgccagttca tccgccgttt
     7021 ggcggttgcc aaacaaaaaa cgggcctcta aaaacgctgt gtcccccgta atatcattgc
     7081 gcacgctttc gcacagttcg tcaatgccgc cgctttttac agacggcatc agtttgcagt
     7141 cccacagggt ttggacaaac cgggcaatct gttcctgaat gccgtctgaa agcggggcag
     7201 gggagacaac cgccaaatcc acatccgaac agggatacag ttcgccgcgc ccgaagccgc
     7261 ccaccgccat cagacataac gcgctgtttt gaaaatgttc tacccacaat gctgccagta
     7321 aggtttcgac tgccgccgtg tattccctga aaaataccga cacgcggttg gttttcaaat
     7381 aatgcgcttc ggcggcatcg cgctgctgtt tgaaggtttc cagtgctgaa gacaggtttg
     7441 caggcatttt tattctttcg attggcggga aaaagggagg cggatggttc agcggtcaaa
     7501 taccgctttc agacggcatt tgtcgggtat cggcggaatt ggtcatcagg ttcaaccgtt
     7561 cctgcggcgg cagcagcaac tgatacgccg ccacgcccaa ggccgccgcc attttttcga
     7621 tattcgacag ggcgatgttc cagcgtttgc gctcgactgc cgacacataa gtcctgtcca
     7681 aaccgcattg ccgcgccaat tcttcttgcg accaaccctt gttcacgcgg aaaagccgca
     7741 tattgtatgc caataccgcc cgcaaatcct gctcgtcagg cagttcggca ggcagagtca
     7801 atttgttgcc catcatttgt ttccggataa atgttaaagt taagcatttg ctgttacgga
     7861 ttttacttca cataaaagct aaaaaaaagg ggtggcggca gccacaccca aacacacaca
     7921 catcaagagg aaagaggtaa aatcaagaca gattgggaaa cagcaaacaa acaatctaga
     7981 tttcatgtcg gtattatcaa acaaaaaccg ttatcgagta ttgattcaaa tcaatttcga
     8041 ttttattcgc aatccgataa agaaaaacgg catatccgtt tatatggata tgccgtctga
     8101 tggtgcaggg gactgctata tttggcagtg atttgtgttt tagtcctctt gcgccgctgt
     8161 ttgcaggtag ttcggcaaac cgattttgcc gatcagttcc tgctgggttt cgagccagtc
     8221 gatgtgttct tcgttggtgt ctttttgttt ttccaacaaa tcacggctga cataatcctg
     8281 ttgcgcttct gccacggaaa ttgccgcaat caatgcctca tgtttttctt tctctttttc
     8341 caaatcgcag gcgatgattt cttcggtgga ctcgccaatc agaagcttgc ccagttcttg
     8401 caggttcggc agaccttcga ggaacagaat gcgctcgatc aaatcgtcgg cagctttcat
     8461 ttcaacgatg gactgtttga agaaatgttc gcccagttct tcaaagcccc agtttttcaa
     8521 aatacgggcg tgaaggaaat attggttgat ggttaccagc agcaagccta agtttttgtt
     8581 tagctcgcgg ataaccagac ggtcgccttt catacggact ccttttattc cgaatacgga
     8641 ttacagttgg ctttggtagt agttgccttc gccaattaac tcgatcaggc gaagctgctg
     8701 ttccagccag tgggcgtggt cttcttcggt atctttcagt tgggcaacca tcaggtcgcg
     8761 cgtaacatag tcttgagcct cttcgcacag tttgatgcct tttttcaacg catcacgtac
     8821 ttcatattcg gtttgcaggt cggctttgag gcaggaaacc acgtccgtgc cgatattcag
     8881 ttcggcgcgt gccattttcg gcgtgccgcc cagcatcagg atgcggcgga tgaagtcttc
     8941 ggcgtgtgtg gtttcttctt ccatctcgtg gttgagacgt tcaaaaagtt tggtgtagcc
     9001 ccattcggag tagaggcggg agtggataaa gtattggtcg cgtgccgcca gctcgccaga
     9061 cagcaattcg ttcatgtaat ctaaaactgc ttgattgcct tgcataatat ttctcttttc
     9121 ttaatttggg tttcgtgtag ttaaacagtg gatgtagcac tgttcggaat gcgtgcattg
     9181 tatgcaaaag ctgtcggcat gacaaatttt ctatttaaaa tacaaacaat tatcaaaaga
     9241 aatagggcgt attccccgat gccttccaaa tcagtatgct gttttttatc ggttttttgt
     9301 gctgttaaaa ctaataatcc atattatcca taaagaacat ttacacttgt taaccatagc
     9361 taaaagcaaa tacggtacgg ttttttatca aaatttataa tgaatcgttc tcattaaccg
     9421 acagattctt ttgagattat cggattttgg gaataaatta tgccgtctga ggggctttca
     9481 gacggcatag acaattaaag ctgattaaac aacgcggcag acagcagcgt aggaaattat
     9541 tttttacttc ggaacggtat tttgggttta atgatgcccg caaccgccgt ggctttcgcg
     9601 taaggcttcg gcagcctgtt cgtcggcgtg gtagctggaa cgtaccatcg cgccgatggc
     9661 ggcattgctg aagcccagtt cgtatgcttc tttttcaaag attttgaact gctcgggcgt
     9721 aacgtagcgc aggacgggca ggtgtccgtc tgaaggctgg aggtactgtc cgatggtaat
     9781 catttcgata ttgtgcgccc gcatatcgcg cataatttca cgcacgtctt cgtctgtttc
     9841 gcccaagccg accatgatgc cggatttggt cgggatgtgc ggcatcattt ctttataacg
     9901 ttttaataag tctaaagaat gttgataatt ggcaccggga cgggcttttc tgtacaggct
     9961 cggatgggtt tctaggttgt ggttcatcac gtcgggcggg gtttcggcaa ggattttgag
    10021 tgcgatgtcc aagcgtcctc ggaagtcggg gacgaggatt tcgattttgg tgttcgggct
    10081 ggtttcgcgg atggctttga tgcagtcggc gaaatgctgt gcgccgccgt cgcgcaggtc
    10141 gtcgcggtcg acggaggtga tgacgacgta acgcaggttc atggctttga cggattcggc
    10201 gaggtttttc ggttcgtcgg ggtcgagcat attgggccga ccgtgtccca cgtcgcagaa
    10261 cgggcagcgg cgggtgcaga tatcgcccat aatcatgaag gtcgccgtgc ctttgctgaa
    10321 gcattcgccg atgttggggc aggaggcttc ctcgcaaacg gtgtgcatct tttgttcgcg
    10381 caaaatgtct ttgatttcaa agaatttgcg cgatgggagt ttggcgcgta tccattcggg
    10441 ctttttcagt ttttcctgaa gggggacgac tttgatgggg atgcgcgcgg ttttgtccgc
    10501 gcctctgagt ttgatgccgc gtttggggtc gtcggttttg atttcactca ttgttatctg
    10561 ctttcggtgt gaattgtgtt tcaaggtgtg cggtaagttt ggcggcgact tcgtccggcg
    10621 tggggcaggg ttggacaaaa tccgcgattt gcgtcatttc cataccggcg tagccgcagg
    10681 ggttgatgtg ggtaaacggg cttaaatcca tattgacgtt gagcgcaagg ccgtgataga
    10741 cggaacctcc tttgatacgc agccccagtg aggcgatttt gcgttcgccg acgtaaacgc
    10801 cggggcgttt gggatctgcc gccgcttcga tgccgtattc cgccaatgtg gcgatgatgc
    10861 tgttttcaag cgcggaaaca atgttgcgga cgctggtttt gcgccgtttg aaatcaatca
    10921 tcgtataaac gaccaattgc ccgggtccgt gataggtaat ctgcccgccc cggtcgattt
    10981 ggacgatggg aatgtcgtcg cggatcagca ggtgttcggg tttgccggcc agcccctgtg
    11041 tgaacacggg gggatgttct accacccaca attcatcttc ggtagaggcg gtacggttgg
    11101 cgttgaaagt tttcattgcc tcaaaagtcg gcagatattc aaccaaacct ttatgtatga
    11161 ttttcatctc aaagtaccac tttgaccaat ttgtgcgaag tcagcgcgcg gtagatattg
    11221 tcaagttgtt cttggttttc aacctttact tgtacggtgg cgccggtata gttgcctttg
    11281 ctgctcggac gcgtggtgat gtggtgcgcc tgcgtgtcgg gggcgtggag gcggacggtg
    11341 tccaataccg cctgctcgaa ctcgggatgc accgcgccca ttactttcaa tgggaaggtg
    11401 cagggaaatt cgatgaggga tgttttgttt ttttgttcgg tcatgatgtg ctgccttgtc
    11461 gtgtacggta tgccgtctga aggcgggttt gcctttcaga cggcatcgga tgtgcgttat
    11521 tttagcctaa accgcgataa caggctatcg ggaaggcgga ggcttttttg acggcgcggc
    11581 ggttctgcta tactggcgca caatattatt tttagaaggg tggtttttat gtatcggagg
    11641 aaagggcggg gcatcaagcc gtggatgggt gccggtgcgg cgtttgccgc cttggtctgg
    11701 ctggttttcg cgctcggcga tactttgact ccgtttgcgg ttgcggcggt gctggcgtat
    11761 gtattggacc ctttggtcga atggttgcag aaaaagggtt tgaaccgtgc atccgcttcg
    11821 atgtctgtga tggtgttttc cttgattttg ttgttggcat tattgttgat tattgtccct
    11881 atgctggtcg ggcagttcaa caatttggca tcgcgcctgc cccaattaat cggttttatg
    11941 cagaacacgc tgctgccgtg gttgaaaaat acaatcggcg gatatgtgga aatcgatcag
    12001 gcatctatta ttgcgtggct tcaggcgcat acgggcgagt tgagcaacgc gcttaaggcg
    12061 tggtttcccg ttttgatgag gcagggcggc aatattgtca gcagtatcgg caacctgctg
    12121 ctgcttccct tgctgcttta ctatttcctg ctggattggc agcggtggtc gtgcggcatt
    12181 gccaaactgg ttccgaggcg ttttgccggt gcttatacgc gcattacagg caatttgaac
    12241 gaggtattgg gcgaattttt gcgcgggcag cttctggtga tgctgattat gggtttggtt
    12301 tacggcttgg ggttggtgct ggtcgggctg gattcggggt ttgcaatcgg tatggttgcc
    12361 ggtattttgg tttttgttcc ctatttgggc gcgtttacag gactgctgct ggcaaccgtc
    12421 gccgccttgc tccagttcgg ttcgtggaac ggcatcttgg ctgtttgggc ggtttttgcc
    12481 gtaggacagt ttctcgaaag ttttttcatt acgccgaaaa tcgtgggaga ccgtatcggg
    12541 ctgtcgccgt tttgggttat cttttcgctg atggcgttcg ggcagctgat gggctttgtc
    12601 ggaatgttgg caggattgcc tttggccgcc gtaaccttgg tcttgcttcg cgagggcgtg
    12661 cagaaatatt ttgccggcag tttttaccgg ggcaggtagg cggttccgaa acatatttga
    12721 agcggaatac aaccctgccc gagtttaa
//
LOCUS       DAWEWP010000049        12252 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWEWP010000049 DAWEWP010000000
VERSION     DAWEWP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.049"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            26..262
                     /locus_tag="WGN53_000981"
     CDS             26..262
                     /locus_tag="WGN53_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ0139988.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENLEIRSTLPLIANRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            430..2205
                     /gene="ggt"
                     /locus_tag="WGN53_000982"
     CDS             430..2205
                     /gene="ggt"
                     /locus_tag="WGN53_000982"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ0139989.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSRLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            2424..2619
                     /locus_tag="WGN53_000983"
                     /pseudo
     CDS             2424..2619
                     /locus_tag="WGN53_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2686..3936
                     /locus_tag="WGN53_000984"
     CDS             2686..3936
                     /locus_tag="WGN53_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ0139990.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLENVRKQITALCDKYPVYGA"
     gene            4027..4671
                     /locus_tag="WGN53_000985"
     CDS             4027..4671
                     /locus_tag="WGN53_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0139991.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            4690..6600
                     /locus_tag="WGN53_000986"
     CDS             4690..6600
                     /locus_tag="WGN53_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0139992.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIIQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     RVGHELENGSGEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKVQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELNPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            6621..6986
                     /locus_tag="WGN53_000987"
     CDS             6621..6986
                     /locus_tag="WGN53_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139993.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            7097..8572
                     /locus_tag="WGN53_000988"
     CDS             7097..8572
                     /locus_tag="WGN53_000988"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ0139994.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPHFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVVDNLSGKIYLIVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSSLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIHAAQMVQEGLDK"
     gene            8569..9114
                     /locus_tag="WGN53_000989"
     CDS             8569..9114
                     /locus_tag="WGN53_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ0139995.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            9111..10247
                     /locus_tag="WGN53_000990"
     CDS             9111..10247
                     /locus_tag="WGN53_000990"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ0139996.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPGSDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            10294..10776
                     /locus_tag="WGN53_000991"
     CDS             10294..10776
                     /locus_tag="WGN53_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0139997.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            10882..11937
                     /locus_tag="WGN53_000992"
     CDS             10882..11937
                     /locus_tag="WGN53_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0139998.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            11934..12146
                     /locus_tag="WGN53_000993"
     CDS             11934..12146
                     /locus_tag="WGN53_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0139999.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGALQGRITFLNHPF"
BASE COUNT         3376 a         3349 c         2789 g         2738 t
ORIGIN      
        1 tgaaaggcac gccccagttt ccccaatgtg gattttcagc gcgtgcagta gaggtgctga
       61 cccaattggc agaccctttg cttttgttaa tatcctagaa aacctagaaa ttcgcagtac
      121 tctaccactc atcgccaatc ggccaacctt tccacagctt tgggttaatg gtgagttaat
      181 cggtggtagt gatattatcc tacaaatgta ccaatcaggt gagctaaaac cactcattga
      241 agagcatagc ccaaaggtct gattacttaa tatcttaaaa taaatatcaa aaaagactgc
      301 tgatgacatt agcggtcttt ttttgatggt caagattgat agtttggatt gaattattta
      361 gccatattaa atacaataat cagcatgcct tgtatgaatc atcaatcaaa ctcaggcgaa
      421 ggagtgcttg tggctaaaac atatttattg actgcattga taatgtctat gacaatctct
      481 ggatgtcaag tcatccatgc caatcaaggt aaggttaata ctcattctgc tgtcatcaca
      541 ggtgcagacg ctcacacgcc tgaacatgca acgggactga ccgaacaaaa gcaggtgatt
      601 gcaagtgatt ttatggtagc gtcagccaat ccattagcaa cacaagctgg ctatgatatc
      661 ttaaagcaag gcggtagcgc tgcagatgcg atggtggcgg tgcagacgac actaagcttg
      721 gtagagccac agtcgtcagg cttgggcggt ggtgcatttg tgttgtattg ggataatacc
      781 gccaaaacat tgaccacatt tgatgggcgt gagacggcac cgatgcgtgc gacgccggaa
      841 ttatttttgg ataaagatgg tcaaccattg aaatttatgg aagcggtggt cggtggtcgc
      901 tcggtgggta cgcctgctat ccctaaactg atggagacga tacatcagcg atacggtgta
      961 ttgccttggg gaaaattatt tgatacgccg atccgcttgg caaaacaagg ctttgaggtg
     1021 tcgccaaggc ttgccatctc ggttgagcaa aatcagcagc atttggcacg ctatccaaaa
     1081 acagccgctt attttttgcc gaatggtgtg ccgctacaag caggcagctt gctgaaaaat
     1141 ttagaatttg ctgacagtgt tcaggcgtta gcagctcaag gtgcaaaagc tctgcatact
     1201 ggtaaatatg cccaaaatat cgtttcagtt gtccaaaatg ctaaggataa ccccggtcaa
     1261 ttatccttac aagatttatc tgattatcaa gtggtggaac gcccgcctgt ttgtgtgact
     1321 tatcgtattt atgaagtatg cggtatgggt gcaccaagct caggtgggat tgctgtgggt
     1381 cagattttgg ggattttaaa tgaattttca cccaatcagg tggggtatga tgctgaaggt
     1441 ttacgtctct tgggcgacgc ttctaggctt gcgtttgcgg atcgtgatgt atatttgggc
     1501 gaccctgatt ttgtaccagt acccattcgc cagttgattt ctaaagacta cctaaaacat
     1561 cgcagccggc tgcttgagca gtcggataag gcattgccta gcgtatctgc aggcgatttt
     1621 attcatgagt gggtgtcctc acaagcgatt gagttacctt ctaccagtca tatatcaatt
     1681 gtggataagg cgggcaatgt gctatcaatg acgacttcca ttgaaaatgc gtttggctca
     1741 actttgatgg caaatggcta cctgctgaat aatgagctga cagactttag ttttgagcca
     1801 ataaagcagg gcaaacaggt cgccaatcgt gtcgagcccg gcaagagacc acgctcctca
     1861 atggcaccaa ccatcgtatt taaagcaggc aagccttata tggcaatcgg ctccccaggt
     1921 ggtagccgta tcattggcta tgtcgctaag acgattgtgg cacatagtga ctggaatatg
     1981 gatatccaag atgccatcag tgcacccaat ttattaaatc gctttggtag ctatgaacta
     2041 gaaacgggta caaccgccct ccagtggcaa caagcattaa atgatttggg ctataagact
     2101 gacgtgcgtg agctaaattc aggtgttcaa gccattatta tcgagccaag caggctggtt
     2161 ggtggtgccg atccacgccg agaaggtcga gtgatgggtg attagaggtg aagcttgtat
     2221 tttgtgaatt tgaccgcatt aagttaatat taaaataggg ggaaattgtg agtaatcaaa
     2281 caaaaataca agccatcaag caagcttctg agcagatctt cgcaatttgt gagacgccaa
     2341 acacagcatt acaagccatt catctgattt taagacatgg tggggcagga gagttgtctt
     2401 ggcaagtggt gtatcagcgg gttatggctg ataaagatgt ggttggtgct ggttatttga
     2461 ttgattttgc acaaactgcc gaaaatttac catttgatgc attgcctttg ggtagccttg
     2521 tactaacata aaggtaatga gacgctaaaa accagcatgt taaataaatt acctgataat
     2581 gccaaagaaa atttacgcat tatggactat ctgccttagg ttttgaagta tttttttata
     2641 caaaaaacac tcatgatacc ctgattacac aaaggagccg agaacatgtt ttcaaaaagc
     2701 gttaccctcg cacaatatga ccccgatttg gcagcagcca ttgcccaaga agaccaacgc
     2761 cagcaagacc acgtcgagct gattgcctct gaaaactacg tcagctgcgc cgtaatggaa
     2821 gcgcaaggtt cccagctgac caacaaatac gccgaaggct accccggcaa acgctactac
     2881 ggcggctgcg aatacgtcga tattgtcgaa caattggcaa tcgaccgcgt caaaaaactg
     2941 ttcggcgcgc aatacgccaa cgttcaaccg cactccggct cgcaagccaa ccaagccgtg
     3001 tacgcttccg ttttaaaacc gggcgacacc attttgggta tgtctttggc gcacggcggc
     3061 cacctgaccc acggcgcgag cgttaatatt tccggcaaac tctacaatgc cgttacttat
     3121 ggtttggatg aaaacgaagt gctcgattat gccgaagtgg aacgcttggc tctcgaacac
     3181 aaacccaaaa tgattgtggc gggtgcgtct gcctacgcgt tgcaaatcga ctgggcaaaa
     3241 ttccgcgaaa tcgccgataa agtcggcgca tacctctttg tcgacatggc gcactacgcc
     3301 ggactgattg ccggtggcga atatcccaac cccgtgccat tctgcgactt cgtgaccacc
     3361 accacccaca aaaccctacg cggccctcgc ggcggcgtga ttctgtgccg cgacaatacc
     3421 cacgaaaaag cactgaactc ttccatcttc ccaagcctgc aaggcggtcc gctgatgcac
     3481 gtcatcgccg ccaaagccgt agcgtttaaa gaagcgttgc aacccgagtt caaacaatac
     3541 gcaaaacaag tgaaaatcaa tgctgccgcc atggcggaag aactggtcaa acgcggtttg
     3601 cgcatcgttt ccggccgcac cgaaagccac gttttcctcg tcgacctgca accgatgaaa
     3661 attaccggca aagccgccga agccgctttg ggcaaagcgc acatcaccgt caacaaaaac
     3721 gccattccga acgatccgga aaaacccttc gttacctccg gcatccgcat cggctccgcc
     3781 gccatgacca cacgcggttt taacgaagcc gacgcacgcg tattggcgaa cttggttgcc
     3841 gacgtattgt ctaaccccga agacgaagcg aatctggaaa acgtccgcaa acagattacc
     3901 gccctctgcg acaaataccc cgtttacggc gcgtaaactt ttttaaaaac aaatgccgtc
     3961 tgaaaatttc agacggcatt tttccaatcg gttaaaatac gcggtttgtt tttttaggaa
     4021 cgcgccgtgc ttgcctccgc catcgacttc atcctccata tcgaccaaca cctgctcgcg
     4081 ctgtcggcgc aatacggtgt gtggatttat gcgattctgt ttttgattgt tttttgcgaa
     4141 accggcctga ttgttacgcc cttactgccc ggcgattcgc tgctgtttgc cgccggcggg
     4201 attgccgcgc tgggcggtat ggatattcat ttgatggttg cattattaag ccttgccgcc
     4261 atactcggcg acgcgctcaa ttttaccgtc ggcaaatatt tcggcggcag gctgttcgcc
     4321 aaccccgatt ctaaaatctt ccgacgtgaa tatctcgata aaacccaccg tttttacgaa
     4381 aaacacggcg gcaaaaccat catcattgcc cgttttatgc ccatcgtccg cacctttgcc
     4441 cccttcgtcg ccggtatggg gaaaatgcac tatgccaaat ttatccgcta caacatcatc
     4501 ggtggcttgt tgtgggttat cctgttttct tacgcgggct atttctttgc caacttcccc
     4561 gtcgtaaaaa acaatctggg cttggtgatg ggcggcatca ttattgtttc cgtcctgccg
     4621 ggtgcggttg aaattgcccg cgccaaactt gcggtaaaat ccaaacggta actgttgaga
     4681 atttgcatca tgaatatatt atctgtagaa aacgcttctt ttgccatcgg ccacgttgcc
     4741 ttgctcgaca aaacttcttt tcaactcgac agcggcgaaa aagtcggctt gatcggacgc
     4801 aacggcgcag gtaagtcttc gtttctgaaa atcctcgccg gcgtgcaaaa gctcgacgac
     4861 gggcagatta tcattcaaaa caacctcaaa atcgtttatg taccgcagga atcctttttt
     4921 gataaggacg caaccgtatt tgataccgtt gccgaaggtt tgggtgaaat tcgcgattta
     4981 ttgcgccgtt accaccgcgt cggccatgag ttggaaaacg gttcgggtga ggctttgttg
     5041 aaagaactca acgaattaca acttgaaatc gaagcgaagg acggctggaa gctggatgcg
     5101 gcagtcaagc agactttggg ggaactcggt ttgccggaaa acgaaaaaat cggcaacctt
     5161 tccggcggtc agaaaaagcg tgtcgccttg gcgcaggctt gggtgcagaa gcccgacgta
     5221 ttgctgctgg acgaaccgac caaccatttg gatatcgacg cgattatttg gctggaaaat
     5281 ctgctcaaag cgtttgaagg cagcttggtt gtgattaccc acgaccgccg ttttttggac
     5341 aatatcgcca cgcggattgt cgaactcgat cgcggtattt tgcgttccta tcccggctcg
     5401 ttctccaaat acagcgaaaa aaaagtgcag gaattggcgg ttgaagcgga acacaaccgc
     5461 ctgtttgaca aattccacgc gcaggaagaa gcgtggatac gcaaaggcat cgaagcgcgc
     5521 cgcacccgca acgaaggccg cgtgcgccgt ttggaagagc tgcgccgcca acgcgccgaa
     5581 cgccgcaatg tgcagggaca ggtcaacttc aagctcgaca gcggcgagaa aagcggcaaa
     5641 atcatcgccg aattggaaca tgcctcgttt gcctatggcg acaaagtcat tatggacaaa
     5701 ttctccgcga ttttgcagcg cggcgataaa atcggtctaa tcggccccaa cggcatcggc
     5761 aaaaccacat ttttaaaact gattttaggc gaactcaacc cgacttacgg cagaatccgt
     5821 atcggcagta agcaggaagt tgcctatttc gaccaattcc gcagcacgtt gaacgaaaac
     5881 gacaccgtgt tttacacgct cggtcaaggc aacgattacg tcgaagtcgg cggcaagaaa
     5941 aaacacgtaa tgagctatct ggaagatttc ttattccatc cagcccgcgc acaaagtccc
     6001 gtttcatcgc tctccggcgg cgaacgcaac cgccttttgc tggcaaaact ctttacccgt
     6061 cctgctaata ttttgatttt ggacgaaccg accaacgact tggatatcga tacccaagag
     6121 ctgcttgaag atttgctgcg cgattatcaa ggcacggtat tccttgtctc acacgaccgt
     6181 atgttcctcg ataacgtgat tacccaaagc attatttttg aaggaaatgg aaggttaaaa
     6241 gaatatatcg gcggctatca ggactacatc gacgcaaaat cacgcgaagc gaagattcag
     6301 acggaatccg cacccaaaac agccgatgcc gaaccggtga aagaaaaacc caaagccaac
     6361 cgcacggtca aactgtccta caaagaacag cgcgaactcg acgccctgcc cgacgaaatc
     6421 gccgccttgg aagccgaaca ggcagaaatc aacgcgcagc tttccgatcc cgaaatcttc
     6481 aaagattacg aaaaagccgg cgcactgcaa agccgcgccg aagaaatcga aatgctgctt
     6541 ttggaaaagc tggaacgctg ggagctgctg gaaaccaaac agaacggcaa tgccgtctga
     6601 aatcaaacag gaagaaaaaa atgttcaaac gacctgaaga aatcattgtc ctgatacttg
     6661 ccgtcctctg gattgccggt acgtattttc tcgccgccct gtttggagcg gatgcttata
     6721 ccgtgttaaa aatcactgcc cttaccctgc tttggtctgc cgcatcattc ctcctttggc
     6781 aaaaaaacct cagccggcat atctggccgc tgctgctcgg ctgcctgact gcctgttggt
     6841 ggccgtatct gaatcaatcg gtcgcacccg gttctttacc ttggtatgca tcatggacgt
     6901 tcaaaaccat cttagccctg attcccgtaa ccgcaggtta tctgtttaaa tggaaacaat
     6961 accggcagcg caaaacaaag ccgtaagccc gtccttttca gacggcatct ccggtcagac
     7021 tgtcaacaat tccgataaaa tgctatactc gcgcccgttg ccgcgccgtc cgaaaccgcc
     7081 ctttgggaaa gcagaaatga tcagcaaaca agaataccaa gcccaagccg ctcaaggcta
     7141 caaccgcatc ccgctcgtgc aagaactcct tgccgatttg gatacgccgc tttccctcta
     7201 tctcaaactc gccaaccgcc cctataccta cctgctcgaa tccgttgtcg gtggcgaacg
     7261 tttcggccgc tattctttta tcggcctgcc ttgcagccac tatctcaaag ccagcggcaa
     7321 acacgtcgat gtttatcaaa acggcgaaat tgtcgagcaa cacgacggca accccctgcc
     7381 ctttatcgaa gccttccaca accgcttcaa aacacccgaa atcccaagcc tgccgcactt
     7441 taccggcgga ctggtcggct acttcggtta cgaaaccatc tacaattttg aacacttcgc
     7501 ccaccgcctg aaaaacacca ccaaagccga cccgctcggc acgcccgaca tcctcctgat
     7561 gctctcgcaa gagcttgcag tagtggataa cctaagcggt aaaatctacc tgattgttta
     7621 cgccgatccg tcgcagcccg acggctacga acgcgcacgc gaacgcctcg aagacatccg
     7681 tacacagtta cgccaaagtt gcgccatccc gctttcgctc ggcagcaaac acaccgaagc
     7741 cgtcagcgaa ttcggcgaag agccgttcaa agcctgcgtt aacaaaatca aagactacat
     7801 ctttgcaggc gactgcatgc aggttgtccc cagccagcgc atgagcatgg aattcaccga
     7861 cagctcgctc gccctctacc gcgccttgcg cacgctcaac ccttcgcctt atctcttcta
     7921 ctacgatttc ggcgatttcc acatcgtcgg ctcctcgccc gaaatcctcg tccgccgcga
     7981 acgcgacgat gtcatcgtcc gccccatcgc cggcacgcgc ctgcgcggca aaacccccgc
     8041 cgaagacctt gccaacgaac aagatttgtt aagcgatgcc aaagaaatcg ccgaacacgt
     8101 catgttgatt gacttaggac gcaacgacgt cggccgcatc agtaaaaccg gcgaagtcaa
     8161 agttaccgac aaaatggtga ttgaaaaata ctcccatgta atgcacatcg tttccaacgt
     8221 cgaaggccgt ctgaaagacg gcatgaccaa catggacatc ctcgccgcta ccttccctgc
     8281 cggcacactc tccggcgcac ccaaagtccg cgccatggaa atcatcgaag aagtcgaacc
     8341 aagcaaacgc ggcatctacg gcggtgccgt cggcgtatgg ggtttcaaca acgacatgga
     8401 tttggcaatc gccatccgca ccgccgtagt aaaaaacaac acgctatacg tccaaagcgg
     8461 cgcgggcgtg gttgccgact ccgatccggc ctccgaatgg caggaaacac aaaacaaagc
     8521 ccgagccgtc atacacgccg cgcagatggt gcaggaaggg ttggataaat gacttatgaa
     8581 aaactttatg agcttcgcca aacactcaga ccgactacca aagacggtct ctatacagat
     8641 aatgaaaaaa gtcgagaaat cctaacggtt agatggagcg gcaacacaga aaatcctaaa
     8701 aatttctcag ctgttgctat aggaattaat ccaagtaaag caaatgatga aagaagcgac
     8761 aaaaccctta cccaacttgc acgtttcttg gatatgtacg gttttaccaa cttcaaaatg
     8821 ttaaatattt tttccagtta ctctacacag caaacaggaa ttagggcaaa cacccaaacc
     8881 gatttttcca aatttaaagg atgtttggaa gatgctgata tgattattct tgcatggggt
     8941 acagaccgcg gtgcttataa ggatgaaaaa aacagaatac tggaatttct aaaagcagaa
     9001 aaatttatgg aaaaagtatt ctgtatttct gaaactggta attccagcga tacaagacat
     9061 ccgtcaagaa ttagttatag ctatcaactt gtacaatttg aagagtcagc atgaaaaaca
     9121 tatctctttc tccgcccgcc atgcttggca tcctcggcgg cggacaatta ggcagaatgt
     9181 ttaccgttgc cgccaaaacc atgggctaca aagtaaccgt tctcgacccc gatccggacg
     9241 cgccggcagc agaatttgcc gactgtcatt tatgcgcgcc gtttaacgac caagctgctt
     9301 tggacgaatt ggcaaaatgc gcggcggtga ccactgaatt tgaaaacgtc aatgccgatg
     9361 cgatgcgttt tctcgccaaa cacaccaatg tttcccccgg cagcgactgc gttgccatcg
     9421 cgcaaaaccg cattcaggaa aaggcatgga tacgcaaagc aggattgcaa accgcgccgt
     9481 atcaagcaat ttgcaaagcc gaagacatca ctgaagaaag catacaattt ctgcccggca
     9541 tcctgaaaac cgctacattg ggctatgacg gcaaaggcca aatccgcgtc aaaacggtgg
     9601 atgaactcaa agccgcgttt gccgaacacc gcggcgtgga ttgcgttttg gaaaaaatgg
     9661 tggacttgcg cggcgaaatt tccgttatcg tatgccgtct gaacaatgac aacgtgcaaa
     9721 ctttcgatcc tgccgaaaac attcacgaaa acggtatcct cgcctactcc atcgtcccag
     9781 cccgactgag tgccgacatt cagcaacagg cgcgacaaat ggcgcagcgt ttggccgatg
     9841 aattgaacta cgtcggcgta ttggcggtag aaatgtttgt tgtcggcgac acgcatgaat
     9901 tggtcgtcaa cgaaatcgcg ccgcgtccgc acaattccgg ccaccatacc gtcgacgcct
     9961 gcgcggcaga ccaattccag caacaggtcc gcctgatgtg caacctgcca cctgctgaca
    10021 ccaaattgct gagttcttgc tgtatggcga atattttggg cgacgtttgg caggaagacg
    10081 gcggcgaacc ggattggttt cccctgcaaa gccggccgga cgcgcacctg cacctttacg
    10141 gcaaaaaaac cgcgcacaaa gggcggaaaa tgggacactt taccgtttta accaccgatt
    10201 cggacaccgc atttcaagaa gcaaaaaaac tgcatcagtc cctataatcc gaccaacggc
    10261 acagatgccg tctgaagccg ccgacatact gccatgaacc tgctgacact gttacgcccc
    10321 gccaccgtgc aagactgcaa agacatcttc aaagtccacc tgcactccgt ccaatacacc
    10381 tgcatcctca gctacaacga acacgcccta aaagtatggg aaggcttgtt aaacacagaa
    10441 agctacctgc cgaccatttc cgatcccgac aaagcacttt gggttgccga atacaaaggc
    10501 aacatacagg gatttttcca aatcgactgc caagaagcac aactcgatgc actttacgtc
    10561 caccccctgt tccacaacct cggactgggc actgccctgc ttcatcaggc ggaaaccatc
    10621 gcccacaaat ccggtttgag ctttctgaaa ctctacgcat cgctcaactc cgtcccgttt
    10681 taccttctca accgctacga atcgctcggt tcggcagtat tgcagcttga ccccagcatc
    10741 aaaatcaaat gcgaactgat gcggaaacac ctctgatccg tccttataac caaaactgat
    10801 aatcttcaaa tcaaacatta tttccacaag cgcagacagc cgtataaagt tgccccatct
    10861 accaaccgac aggacaagca tatgaaaacc tacgcaccgg cactctatac cgcagccctg
    10921 ctcaccgcct gcagccccgc agccgattca aaccatccgt ccggacaaaa tgccccggcc
    10981 aataccgaat ccgacggaaa aaacattacc ctgctcaacg cctcatacga tgtggcacgg
    11041 gatttttaca aagaatacaa ccccttattt atcaaaacat accaatccga acaccccggc
    11101 acatccgtca gcatccaaca gtcccacggc ggctccagca aacaggcatt atccgtagcc
    11161 aacggccttc aagccgatgt cgtaaccatg aaccaatcct ccgacatcga cctgctcgaa
    11221 aaaaaaggac tggtagaaaa aggctggcaa caagccctcc ccgaccacgc tgcgccctac
    11281 accagcacta tggttttcct tgtccgaaaa aacaacccca aacagatccg cgattggaac
    11341 gaccttgcca aagacggcgt taacatcgtc atcgccaatc ccaaaacctc gggcaacgga
    11401 cgctacgcct tcctcggcgc atacggttac ggtctgaaaa ccaccaacgg caacgaacag
    11461 gaagcccaaa aactcgtcgc atccatcctc aaaaacaccc ccgtttttga aaacggcgga
    11521 cgcgccgcca ccaccacctt cacacaacgc aacatcggcg acgtactcat tacttttgaa
    11581 aacgaagcca actacgtcag caaaaaactg acccaaggac agtttgaaat cgtctatccg
    11641 agctacacca tttccgccga aagccccgtc gccgtcgtca acagcgtcgt cgcgaaaaaa
    11701 ggcacgcaaa aaaccgcccg cgcctatctc gaataccttt ggagcgaacc ggcgcaagaa
    11761 ctcgccgcct cactctacct gcgcccccgc aaccccgaag tattggcaag acacaaagcc
    11821 gacttcccag acttagactc attctctccc gaagaaaaat tcggcggatg ggacaacatc
    11881 atgaaaacct acttcgccga cggcggcata ttcgaccgcc tgaccgcaca aaaatgaccg
    11941 ctatgccgtc tgaaccgcct ttcagacggc attccacaca tcaaaacaga aaaaccgcgc
    12001 ccaaaatcat catcacaaga cacaacggca aaccggatac aaaacccaac accgccgcca
    12061 aaatgccgtc tgaagccgtc ggcgcaacca tccttatctg gggcgcgctt caggggcgca
    12121 taactttcct taaccatccc ttttgacagc caaataaaca gtgcttttgc ctgtcagaaa
    12181 caaaaccaat attttgcagt tcgtatcgga acatttatag tggattaaca aaaaccagta
    12241 cagcgttctc gc
//
LOCUS       DAWEWP010000050        11769 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWEWP010000050 DAWEWP010000000
VERSION     DAWEWP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.050"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(142..939)
                     /locus_tag="WGN53_000994"
     CDS             complement(142..939)
                     /locus_tag="WGN53_000994"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ0140000.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            1094..3940
                     /gene="uvrA"
                     /locus_tag="WGN53_000995"
     CDS             1094..3940
                     /gene="uvrA"
                     /locus_tag="WGN53_000995"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ0140001.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMESGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAQCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGIPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADYIVDLGP
                     EGGDGGGRIIASGSPEEVAKVKGSYTGKYLKVVL"
     gene            3955..6009
                     /locus_tag="WGN53_000996"
     CDS             3955..6009
                     /locus_tag="WGN53_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0140002.1"
                     /translation="MSSNYFFSAIDESLITSNNSLSMFLNNLKNYSLDQKNHIFVIQA
                     PLANNKYIYTYKDVVIVLIAKKKIAFINFGDTDSFETFREDFIEDLNAISDKYEYKSK
                     IGRPRDWKTIFKNFSLDELVSNDIQNFVKNIEISSEREQRLSELIISLLTGSINDIEK
                     IDGLNIQDNILDKIKKKIILFDSNQTNFLYQNKNQKKIIIQGLSGTGKTELLLHKLKE
                     LYLESDNNRIFFTCNNKILAESLRNRIPRFFNFMQVSQQIDWDNRLWCNHAWGSGKNK
                     NSGLYSFICHFYGIPFLNYAQESSFSKICSMAIEQIKSNSDNLNKYAFDYILIDESQD
                     FGESFFELCSLVTKHKIYIAGDIFQSIFGEQKIYDHVDYLLNQCYRTDPKTLMFSHGL
                     SMGLFESEKLQWLTDQQWEQYGYIFQKNNNRYIFTREPIRRFEDLKDNDNSIHIENIK
                     ELNNSQISDKIVELIMDIKSKYPSVQPDDIAIIFLEQNRRYNSEMLNYIRYTLEEKGI
                     DWPINPAFESKQQLTGYLSITNFNHVKGLEFPFVICISPYEVTADLRKRNAIYMAMTR
                     SFLHSSLLMPVSTDNFFLYSRGLNEINSTKRMTIEEPNEENKNRIREINIQFDGSHSF
                     KDIVIEILDSFPKSSSITDKDREWCIQGVLRSLGETYDKNAIKSHLKPLIDYIQGERN
                     ES"
     gene            5999..6667
                     /locus_tag="WGN53_000997"
     CDS             5999..6667
                     /locus_tag="WGN53_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140003.1"
                     /translation="MKADFFILDGNHLSRFKYIFQEAIRDKILVLRILFESIDYILHL
                     PKSDRYLNDYNKIGDYTIILSINKHSRIIFYSNQKIFSIVLPFTYFNDKKTYEFYFNS
                     ILINSGIVSYIKEFLDMLSKNGNTLVLDEIMEFYTNNPEKDSDKDNAIIILSDLILLE
                     DGYIRYDYDPEHENGNLHPLHHYDIFYQNKNTFKIGLKSAIGCKEFIELLDCTEERSF
                     LSKL"
     gene            complement(6781..6966)
                     /locus_tag="WGN53_000998"
     CDS             complement(6781..6966)
                     /locus_tag="WGN53_000998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140004.1"
                     /translation="MFDKQARFWIGEFDGEKECAAGNEVSAITRHRIFVFGLNVGEGT
                     VCCLGFNFVEVEVFRRH"
     gene            6989..7318
                     /locus_tag="WGN53_000999"
     CDS             6989..7318
                     /locus_tag="WGN53_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0140005.1"
                     /translation="MDVQKQYPFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPYTKNLSAGKQRQHERGIWQRRFYGHTVRDETDLQRCADYIHSNPIKQKMSRA
                     KTESCKK"
     gene            complement(7553..8443)
                     /gene="sucD"
                     /locus_tag="WGN53_001000"
     CDS             complement(7553..8443)
                     /gene="sucD"
                     /locus_tag="WGN53_001000"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ0140006.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPDECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            complement(8454..9620)
                     /gene="sucC"
                     /locus_tag="WGN53_001001"
     CDS             complement(8454..9620)
                     /gene="sucC"
                     /locus_tag="WGN53_001001"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ0140007.1"
                     /translation="MNLHEYQAKELLAGYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(9720..10007)
                     /locus_tag="WGN53_001002"
     CDS             complement(9720..10007)
                     /locus_tag="WGN53_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140008.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAVLWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(10132..11565)
                     /gene="lpdA"
                     /locus_tag="WGN53_001003"
     CDS             complement(10132..11565)
                     /gene="lpdA"
                     /locus_tag="WGN53_001003"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0140009.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
BASE COUNT         3219 a         2869 c         2593 g         3088 t
ORIGIN      
        1 tgataaatca ccgtaacgtt aaatttccag attcccattt tcgtgaacat aacggtgtga
       61 acattagcaa aaattcaaaa aaccaaactt cctcattcgt gtgaatgatg gtactgatat
      121 tttctatttc agttcgcaaa attaatcttt caacacatct tgcacagccg cctgaatctt
      181 agccgccgct gcctcgattt gccgttgttc ggcagatggg tttgccgctg tttcaaccgg
      241 agcagtttgt aaagcaggct cagactcagg ctcggattcg atttgcggcg cagtcagcgg
      301 caaacgcgcc aatacagtgc taacgccagt tactttatcg ccaatcgcca cttgcgcctg
      361 cgcatcgaca ggcagataca tatccacgcg cgaaccgaag cggataaagc cataacgttc
      421 gccgcgggac agtttcgcac ctgcttgggt gtagcacaaa atacggcgcg ccaccaaacc
      481 ggccacttga acaaaagtaa tttcacgacc ggaagccgta gtcgccaaca ccgcattacg
      541 ttcgttttcc gtgctggctt tgtccaaatc cgcattcacg aatttgcctt tgttatagac
      601 cactttcgtt accgtacaat cggcaggcga tttttgcgaa tgcacgttga acacgttcat
      661 aaaaatactg attttcaaag catcgacatc acgatacgga tcgcgtgcgc gctcgaccac
      721 cacgatacgg ccgtcaaccg gactcaacac tgcttcagga ttttgcggaa tctcacgcgc
      781 agggtcgcgg aaaaactgca gggcaaatac ggtaaacacc caaaacggca aagaccacca
      841 gccgcaacac atcgacacca gcaggctcaa agccaaaccg ccgccaataa tcggccagcc
      901 ctcacgggcg ataatcgggt gggggtaaag acggttcatt tgtcatcctt ttgttcaagc
      961 gggttaaaat tgccgacatt atagcggaat ctgccgtact ccgccgacaa atgccgtctg
     1021 aatcctgctt aagacgttat ttgataaagt gctacaatat cggttttcct ttatttttca
     1081 ggcggcatcc attatgtgca accatcatcc ccaacattcg cacgacaacg acaccatccg
     1141 catacgcggc gcgcgcacgc ataatttgaa aaacgtcgat ttagacattc cgcgccacaa
     1201 gctggtggtg gtaacaggat tgtcaggcag cggcaaatca tcgctggcgt tcgacacgct
     1261 gtatgccgaa ggacagcggc gttatgtcga gagcctgtcc gcctatgcgc ggcagttttt
     1321 gcagatgatg gacaaacccg atgtcgattt gatcgaaggc ctgtcgcccg cgatttccat
     1381 cgagcagaaa tccaccagcc acaatccgcg ttccaccgtc ggcacggtta cggaaatcca
     1441 cgactatctg cgccttttgt acgcacgcgt cggcacaccc tactgccccg aacacaagct
     1501 gccgctatcc agccaaaccg tatctcaaat ggttgatgcc gtcttaaaac tgccggaaga
     1561 cacgcgcgtg atgattctgg cgccggcggt acgagagcgt aagggcgagt ttgtcgactt
     1621 ctttgccgac ttgcaggcgc aaggctttgc ccgtgtgcgc gtggacggcg aagtttatca
     1681 gcttgacgaa gtgccgaagc tggagaaaaa catcaagcac aatatcgacg tggtcatcga
     1741 ccgcgtgaaa gtgaaggcgg acatcaagca gcggctggcg gaaagttttg aaaccgcact
     1801 gcgccacggc aacgagcgcg cgctggcgat ggaaatggaa agcggcgaag aacattggtt
     1861 ttccgcgcgt ttcgcctgtc ccgtatgttc atacagcctg cccgaattag agccgcgcct
     1921 gttttcgttc aacaacccga tgggctcctg cccaacttgc gacggcttgg gcaacaccaa
     1981 cttcttcgat cccgaaaaag tggtcgcgca tcccgaattg tcgctggcaa cgggcgcgat
     2041 tgacggctgg gataagcgca accagttcta tttccaaatg attcagtcgc tggcgcggca
     2101 ttacggtttc gatgtgcagg ctgcttggga aacgctgcct gaaaaagtca aaaaagtcgt
     2161 gctgcacggc tcgggcaagg aagtcattga tttcacctac ctatccgaac gcggcaccac
     2221 cttcaaccgc agccacgcct tcgaaggcat cattcccaat ctcgaacgcc gctaccgcga
     2281 aaccgacagc gaaaccgtgc gcgaaaaatt gcgcgaatac cagaaccacc gcgcctgccc
     2341 gagctgcggt ggtgcacgtt tgcgcaaaga agcccgctac gtttacgtca gcggcgaacc
     2401 gctgcacgaa gtttccgcct ggccgctcac caaaacccac caattctttg aaacactgga
     2461 tttagacggc aacaaaaaac aaatcgccga aaaaatcctc aaagaaatca ccgaacgcct
     2521 cggcttcctg attaacgtcg ggctggatta cctgaattta agccgctccg ccgaaaccct
     2581 ctccggcggc gaagcccagc gcatccgcct cgccagccaa atcggcagcg gcctgaccgg
     2641 cgtgatgtat gtactggacg aaccctccat cggcctgcac cagcgtgaca atgaccgcct
     2701 gctggccacc ctcaaacgac tgcgcgattt gggcaacagc gtgattgtgg tcgaacacga
     2761 cgaagacgcc atccgcgaag ccgatttcgt ggtcgatatg ggccccggcg caggcgaaca
     2821 cggcggcaac gtactgattg ccgacacgcc cgaaaacgtc gcccaatgcg aaaactccgt
     2881 taccggacaa tacctcagcg gcaaaaaatc cattgccgtg ccgtctgaac gcacgcccgt
     2941 caatcccgac cggatgctcg tcctcaaagg cgcacgcggc aacaatctca aaaacgttac
     3001 ccttgagctg ccgctcggtt tgattacctg catcaccggc gtatccggca gcggcaaatc
     3061 caccctgatt aacgacaccc tcgccaaaat taccgcccgc gaactcaacc gcgcccaaga
     3121 agaacccgcc ccatacgacg acatccgcgg cctcgaacac ctcgacaaag tcatcaacgt
     3181 cgaccaatcc cccatcggcc gcaccccgcg ctccaacccc gccacctaca ccggcctgtt
     3241 cacccccatc cgcgaactct tcgccggcat ccccctctcg cgcgaacgcg gctacaacgt
     3301 cggccgcttt tccttcaacg tcaaaggcgg ccgctgcgaa gcctgccaag gcgacggcgt
     3361 gattaaagtc gaaatgcact tcctgcccga cgtgtacgtc ccctgcgaag tctgccacgg
     3421 caaacgctac aaccgcgaaa ccctcgaaat ccaatacaaa ggcaaaaaca tcagccaagt
     3481 cctcgacatg accgtcgaag aagcccgcga atttttcgat gccgtcccca ccgtatcgcg
     3541 caaactgcaa accctgatgg acgtaggcct aggctacatc cgcctcggtc agtccgccac
     3601 caccctctcc ggcggcgaag cccagcgcgt caaactcgcc ttggaactct ccaaacgcga
     3661 caccggcaga acgctctaca tcctcgacga acccaccacc ggcctacact tcgccgacat
     3721 cgccctgctg ctggaagtga taggccgtct gaaaggcaaa ggcaactcaa ttgtgattat
     3781 cgagcataat cttgacgtta taaaaacggc tgattatatt gtggatttgg ggccggaagg
     3841 cggcgacggt gggggaagga ttattgcttc aggtagccct gaggaagtgg cgaaggttaa
     3901 ggggagttat actgggaagt atttgaaagt ggttttgtaa ttaggagatt taaaatgagt
     3961 tcaaattatt tttttagtgc tattgatgaa agtttaatta catcgaataa ttcattatca
     4021 atgtttctta ataatttgaa gaattattca ttagatcaga aaaatcatat atttgtgatt
     4081 caagctcctc ttgcaaataa caaatatata tatacatata aagatgtagt tattgtatta
     4141 attgcaaaga aaaaaatagc cttcattaat tttggagata cagattcttt tgaaacattt
     4201 agagaggatt ttattgaaga tttaaatgct atctctgata aatatgaata taaaagtaaa
     4261 attggtagac ctagagattg gaaaaccata tttaaaaatt tctccctaga tgaattagtt
     4321 agcaatgata tacaaaattt tgttaaaaat attgaaattt cttccgagag agagcagcga
     4381 ttgagtgagt tgatcatttc tcttcttact ggaagtatta atgatattga aaaaatcgat
     4441 ggtctaaata ttcaagataa tattcttgat aaaattaaaa agaaaattat tctttttgac
     4501 tctaatcaaa caaatttttt gtaccaaaat aaaaatcaga aaaaaattat tattcaaggc
     4561 ttatcaggca cagggaaaac agaactatta ctacataaat taaaagaatt gtatttagag
     4621 tcggataaca atagaatatt ttttacatgc aacaataaaa tattagcaga aagtttaaga
     4681 aacagaatcc caagattttt taactttatg caagtatcac aacaaatcga ctgggataat
     4741 cgattatggt gtaaccatgc gtgggggtca ggcaagaata aaaattctgg actatatagt
     4801 tttatttgcc atttttatgg tattccattt ctgaactatg cccaagagtc atcattctct
     4861 aaaatttgct ccatggcaat tgaacaaatc aaatcaaatt cagataatct taacaaatat
     4921 gcattcgatt atatattaat tgatgagagc caagattttg gtgagagttt ctttgaatta
     4981 tgcagcttag tgactaaaca taagatctat attgcaggag atatattcca atctattttt
     5041 ggagagcaaa aaatttatga tcatgtagac tacttattga atcaatgtta tagaacggat
     5101 cctaaaactt taatgttttc tcatggcttg agtatggggc tatttgaaag tgagaaatta
     5161 caatggctaa cagatcaaca atgggaacag tacggataca ttttccaaaa aaataataat
     5221 agatatatat ttacaagaga accaattcga cgatttgaag acttaaaaga caatgataat
     5281 agcattcata tagaaaatat taaggaactt aataattccc aaatttcaga taaaattgtt
     5341 gaattaatta tggatattaa gtctaaatac ccaagcgttc agcctgatga catagccata
     5401 attttcttag agcaaaatcg tagatacaat agtgagatgc ttaattatat tagatataca
     5461 ttagaagaaa aagggataga ttggcctata aatccagcct ttgagtctaa acaacaatta
     5521 actggatatt tatcaatcac aaattttaat catgtgaaag gattagaatt tccttttgta
     5581 atctgtatct caccatatga agttacagcc gacttacgga aaagaaacgc tatttacatg
     5641 gcaatgacta gatcattttt acatagtagc cttttaatgc ctgttagcac tgataacttc
     5701 tttttatact ctcgaggact caatgaaatt aattctacta aaagaatgac tattgaagaa
     5761 ccaaatgaag aaaataagaa ccgaattcga gaaattaata tccaatttga tggtagtcat
     5821 tcatttaagg atattgttat agaaattctg gattcatttc ctaaatcgtc atctattaca
     5881 gataaagata gagaatggtg cattcaagga gttttacgtt cattaggaga aacctatgat
     5941 aagaatgcaa ttaaatcaca tctaaaacct ctgattgatt atatccaggg tgagagaaat
     6001 gaaagctgat ttttttatat tagatggtaa tcatttatca agatttaaat atatttttca
     6061 ggaggcaatc agagataaaa ttttagtctt aagaatttta tttgaatcaa tagattatat
     6121 tttacatctt ccgaaaagtg atagatatct gaacgattat aataaaattg gagactatac
     6181 aattatatta tctattaata agcattcacg tattattttt tattccaatc aaaaaatttt
     6241 ttcaatagtt ctaccattta cttattttaa tgataaaaaa acatatgaat tttattttaa
     6301 tagcattctt attaattctg gtatcgtttc gtatattaaa gaatttttag atatgttatc
     6361 caaaaatgga aatacattag ttttagatga aataatggaa ttttatacaa ataatcctga
     6421 aaaagattct gataaggata atgctattat tatattaagc gatctaattt tattggagga
     6481 tgggtatatt cgatatgatt atgatcctga gcatgaaaat ggtaatttgc atcctctaca
     6541 tcattatgac attttttatc aaaacaaaaa tacttttaaa attggtttaa agtcagccat
     6601 cggatgcaag gaatttattg aattactgga ttgcaccgaa gaaagaagct ttcttagcaa
     6661 actgtagtct gcatactcaa cccacagcgt gcgtggctgc accacacacg cgggttgctt
     6721 gattgtcggt ttgccatttt caaatttgct ttcgttgaag ctagcgcttt cagacggcct
     6781 ctaatgtcgt ctgaaaacct caacttcaac aaagttaaac cctaaacaac aaaccgttcc
     6841 ttctccaacg ttcaaaccaa aaacaaaaat cctatggcgc gttatcgcag aaacttcatt
     6901 gccggcggca cattcttttt caccgtcaaa ctcgccgatc caaaatcgcg cctgcttgtc
     6961 gaacatatcg gcttcgcgtg cggcttatat ggatgtgcaa aaacaatatc cctttgaaac
     7021 cgtcgccgta tgcgtgctgc cgaaccatat tcacgccatt tggacgctgc cgcccgacga
     7081 tgcggattat tccctactcc ggcggctgat taaaaccaaa ttctccgcat attcccctta
     7141 tactaaaaac ttatccgccg gcaaacagcg gcagcacgaa cgcggtattt ggcaacggcg
     7201 tttttacgga cacaccgtgc gcgacgaaac ggatttgcag cgttgcgcgg actacatcca
     7261 ttccaatcct atcaaacaaa aaatgtcgcg tgctaaaaca gaatcctgca aaaaataata
     7321 tcagtctttt caaatgcgat agttatcatt ccccaacttc aaacaaggcg tgtgcaacct
     7381 gacttacggg tttgctcctg tttgaccgat aaatattctg cctgcctcaa agggcaaaaa
     7441 taatgtgggt atgttgaggc tgcctctttt tgaaatgaca aacaaaatgc cgtctgaaac
     7501 ccgatttccg gtttcagacg gcatttgatg ttcaatcagt tgtcaaacct gattatgcca
     7561 aaccttttgt tttcaacact tccagcatgg tagtgcccaa ctcggcaggg ctgcgggtgt
     7621 aagcgatacc ggctttttcg aaagcggcga atttttcctc ggcagtacct ttaccgccgg
     7681 aaataatcgc accggcgtgg cccatacgtt tgcctttagg agcggtaaca ccagcaatgt
     7741 aacctacaac aggtttggtt acgttggatt ggatgtattc ggctgcttct tcttccgcag
     7801 taccgccgat ttcaccgatc atgatgatgg cgtcggtatc cgggtcttct tggaagagtt
     7861 tcagtgcgtc gatttgattc ataccaggaa tcgggtcgcc gccgataccg atacaggttg
     7921 attgacccaa gcccagtttg gtggtttgtg ccacggcttc gtaagttaat gtaccggaac
     7981 gggaaataat accgatgcgg cccggagtgt ggatgtggcc cggcataata ccgattttgc
     8041 actcatccgg agtaatcacg cctgggcagt tagggccgac caagcgggta ccgttgccgt
     8101 tggtttccaa ataacgtttg gctttgagca tgtccagagt aggcacgcct tcggtaatca
     8161 caacgaccaa gcctacgcct gaatcaacgg cttcaacgat agaatccaac acaaacggag
     8221 cgggaacgta aattacagat gcatccgcgc cggtttcttt aaccgcttct ttcatggtgt
     8281 tgaatacagg caggttcagg tgggtttgac cgcctttgcc cggggtaacg ccgccgacaa
     8341 ctttagtgcc gtaagccaga gcttgttcgg agtggaaagt accgttttta ccggtgaaac
     8401 cttgaaccaa tactttggtg tctttattaa tcaatacgct cattcttttc tccttaggcg
     8461 tttacggctg cgacaatttt ttcggctgcg tcattcaggc cgtctgcaga agtcagtttc
     8521 agacctgatt cgttcaggat tttcgcgccg agttcggcgt tgttgccttc caaacgaaca
     8581 acgacaggaa cgtcgacgtt gatttcttta acggctgcca cgatggcttc cgcaatcatg
     8641 tcgcaacgta cgataccgcc gaagatgttg atcaatacgc cttgaacgga tttgtcttcc
     8701 agaatcagtt tgaacgcttc aaccacgcgg tctttggttg cgccgccgcc aacgtccaag
     8761 aagttggcag gttggccgcc tttcagtttg atgatgtcca tagtggccat cgccaaaccg
     8821 gcaccgttca ccatacagcc gatgttgcct tccagggcaa catagttcag gtcaaattca
     8881 gaagctttca actcgcgttc gttttcttga gatttgtcgc gcaattcggc gattttcggc
     8941 aggcggtaga gcgcgttgct gtcgatgccg attttgccgt ccacgcaggc gagcgcgccg
     9001 ttttcgcgaa ctgccagcgg gttgacttca aacagggcga agtcattttc gacaaacgct
     9061 ttgtacgcac cggtcatcag tttgacgaac tcgttgattt gtttgtcttt caagcccagt
     9121 tggaacgcaa cttcgcgagc ttggcaaggt tgcaggccga ccagcggatc aacggttact
     9181 ttgaagattt tttcaggagt ttcggcagca actttttcga tttccacgcc gccttcggta
     9241 gaggccatga atgtaatgcg gcgggtagaa cggtcaacca ctgcgcccaa gtacagctcg
     9301 gtttgaaccg gatacatgtc ttcgcaaacc aaaacgctgt tgacaggttg gccgttggca
     9361 tcagtttggt aagttaccaa gttggtgcca atcaggcttt cagccacttc tttagcttct
     9421 tcgcggcttt taacgacttt tacgccgccc gctttaccgc ggccgccggc gtgtacttgt
     9481 gctttgacaa cagcgaattt gccgcccaat ttgtcgtaag ctgcagcggc ttcttcgccg
     9541 ttgtgtgcca aaataccgcc ttgtacgggc aaaccgtagc cagccagcag ttctttagcc
     9601 tgatactcgt gtaaattcat ggatttctcc tttagattag tggaatgccc ccgagtttgc
     9661 ctccaaatgg caagcccgtg cgtttcagac tacctttcgg cggtcggaaa atcgtcgttt
     9721 cacttgctga aaggcaacca catccgcgcc agcagataca tggctgcacc gcctcccccg
     9781 agaataaaaa agacgatgcc ttttttacgc gaattcgggc agagcagata aacggcaagg
     9841 ctgaaactga taaacaacaa tgccacgcgc caaaccaatt ctttatcttt gaaacggttg
     9901 agcgtataag tttcggtcat catcacatac atctgccaca atacggccat acacatgctg
     9961 atgatgcccg ccggtataaa gaaaataccg actatttctt tgttcataat gaatgtaaat
    10021 gctgtttgtt tgtctgacgg aattataaat cagaaaacga agctctcgga acattcaatt
    10081 tgtcggaggc cttaaggccg tctgaaaagt ttcagacggc cttaatgtct atcaaccgtg
    10141 caaagcgcgt ttgtcggccg ccaatgcagc ttcgtgaacc acttcggaca aggttgggtg
    10201 ggcatggata atgcgggcga tgtcttcgct gctggcgaag aattcgagcg cagtcacgcc
    10261 ttcggtaacc aattcgctga caaccggacc aatcatgtgt acgcccaaga tgcggtcggt
    10321 tttggcatct gccaacactt taaccgtacc tttggctttg cccattgcca atgcgcgacc
    10381 attcgcacca aaacctgaag tacctttttt gtactccacg ccttcggctt tgagctgctc
    10441 ttcggtttta cccacccaag cgatttcagg atcggtgtaa atcacgaacg gtacgttgtt
    10501 gaagtcgata tgcggttttt gaccggcaat gcgttcggca acggcaacgc cttcgtcgct
    10561 ggctttgtgt gccaacatcg ggccgcgaac cacgtcgccg attgcccata cgttaggcag
    10621 gttggtacgg cattcgccat ctactttgat aaagccgcgc tcgtcttttt ccaagcctac
    10681 ggcttccgcg ttcaggcctt tggtgtttgg aatacggccg atggcaacga tcagtttgtc
    10741 gaatacttcg gttttggctt cgccagcagc agtttcgtaa gcaacggaaa cacctttgcc
    10801 ttcagacttg atgtcgccga ttttcacgcc caattcgatg ctcagacctt gctctttggt
    10861 gaagtatttg aaggcttctt tggcgatttg ttggtcggcg gcagccagga aggtcggcgc
    10921 ggcttcaaga atggtaactt ccgcacccac gcggttccat acggaaccca tttccaaacc
    10981 aatcacaccg gaaccgatca cgccgagttt ggcaggtact tcggtcaggt tcaatgcgcc
    11041 ttcgttgtcc aatacgttca cattgtcgat agcgacttgc ggcaacggac gcggtacgga
    11101 accggtcgct acgatgacgt gtttggcttc gataacggtt ttctcacctt tgttatcgac
    11161 ttcgatttgg taagcatcgc catttttgcc ggcaaaggaa gccgtaccga acaggctggt
    11221 tactttgttt ttctggaaca ggaatttcac gccgccggtc agtttggtta cgatggcatc
    11281 tttgcgctca atcattttgg ccacgtcgaa ttttacgtcg ccgacagtga taccgtgttc
    11341 ggcaaactcg tgttgcgctg cgtggaaatg ttcgctggat tgcaacaggg ctttagaagg
    11401 gatacaacct acgttcaagc aagtaccgcc caatgcaggg gcattgcctg ctttgttaac
    11461 gcctgcatcg acacaggcag ttttgaaacc cagttgtgcg gcacggatgg cggcaacgta
    11521 tccgcccggg cctgcaccaa tcactactac atcatattga gacatcgtgt catcctttgc
    11581 ttttagcagt atctcaaaat aaaaacgata cttttcgttt cttttttatt ttgaatgact
    11641 gcgttttgat ggttttaaga aaaagccgtc tgaaagtcca tatctgccga ttcattcgaa
    11701 tcaaatgttt cagacggtct caatcgtttg ttgagcctat tgtagcacga tactacatta
    11761 cgctacatc
//
LOCUS       DAWEWP010000051        11760 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWEWP010000051 DAWEWP010000000
VERSION     DAWEWP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.051"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            97..813
                     /locus_tag="WGN53_001004"
     CDS             97..813
                     /locus_tag="WGN53_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0140010.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="WGN53_001005"
     CDS             962..1864
                     /locus_tag="WGN53_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ0140011.1"
                     /translation="MDSLMTLLSVLVPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            1907..2680
                     /locus_tag="WGN53_001006"
     CDS             1907..2680
                     /locus_tag="WGN53_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ0140012.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEAGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="WGN53_001007"
     CDS             2792..4480
                     /locus_tag="WGN53_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ0140013.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="WGN53_001008"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="WGN53_001008"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ0140014.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            5841..6224
                     /locus_tag="WGN53_001009"
     CDS             5841..6224
                     /locus_tag="WGN53_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ0140015.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            6339..6812
                     /locus_tag="WGN53_001010"
     CDS             6339..6812
                     /locus_tag="WGN53_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ0140016.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMSAMDHGHHHGEAHQH"
     gene            6938..8041
                     /gene="mnmA"
                     /locus_tag="WGN53_001011"
     CDS             6938..8041
                     /gene="mnmA"
                     /locus_tag="WGN53_001011"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ0140017.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            8112..9782
                     /locus_tag="WGN53_001012"
     CDS             8112..9782
                     /locus_tag="WGN53_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ0140018.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSISDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGTTAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRINVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVRKDPS
                     LTKEELIAFCRTELTAYKVPKDIEFRDELPKSNVGKILRRELRQSTGK"
     gene            9894..11528
                     /locus_tag="WGN53_001013"
     CDS             9894..11528
                     /locus_tag="WGN53_001013"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ0140019.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         2911 a         3175 c         3112 g         2562 t
ORIGIN      
        1 atttgtgcaa tgctttaatt cctctgacac gatttgaaac acagccgcct taaaacatct
       61 taaaaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccctgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc cggcaaaaag
      241 ctgaccgaat ccgaactttg ccgtcagatg gtgtgttcgc gcaacaccgt ccgcggcgct
      301 ctgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc agatttgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgataa ggcgcgaaga agaggcttcc ggcaggggcg accgcgtcgg ctggaaccgc
      541 ctgtccaatg ccttccacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggaacgct tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca tatatcatgc ggcaataaag gaggtcagga
      961 gatggacagc ctgatgacat tgctttcggt attggtaccg atgttcgccg gattttttat
     1021 ccgtgtgccc aagccttacc tgcccgcttt ggacaaggtg ctgtcggttt tggtgtatgc
     1081 cgtgctgctg ctgatcggcg tatcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcggggcga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggcgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcattcgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaagcgcg ggcatgtatt gtctgatgct
     1381 gctggtgttc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcgggcgg
     1501 gctgctgttt gccgcatcga cagacggtgt gtcgtggacg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtcatgacc gaggcttacg gcgcggtatg
     1621 gggcagcatc atgctgctga acgatttggc acgagagctg tttgcactgg catttatccc
     1681 gctgctgatg aagcgttttc cggatgcggc ggtgggggtt ggcggtgcga ccagtatgga
     1741 ttttacattg cccgtgattc agggtgcggg cggtttggaa gtcgtgccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata ttgccgcccc gtttctgatg gtggtgtttt ccacgctggg
     1861 ctgaacacgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgagttgga
     2041 ggaggccggc agcctgaaac gccatcacgg cggcgcatct tcgggcggaa acttgccgga
     2101 gggcctgccc gccgaccgcc aaacccggtg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa cacatccccg acggttcgtc gctgtttgtc agtatcggca cgacgatgga
     2221 ggccgtggtg tcggagctgg tcaggcggcg cggcagcctg agggtgatta ccaacaatat
     2281 ccacgccgcc tcgattgctt cggcgcggac ggattatacg gtcattatca cttctggagt
     2341 cgtccgtcct ttggacggcg gtattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaatgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgcc tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat ttgcggacaa
     2821 ccattttatc cgcaccttca tcgaagaaga cctcaaaagc ggcaaacacg aaaccatcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggcgt atatttacga cggcttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagacg ttgagtggct
     3061 cggtttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttggtta aagacggcaa agcgtatgtc gatgatttga cgccagaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcaggcaaa aacagccctt atcgcgaccg
     3241 cagtatcgaa gaaaacctcg acctgttcac gcgcatgaaa aacggtgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatccggc aacatcaata tgcgcgatcc
     3361 ggtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgcatcta
     3421 tccgatgtac gactacacgc attgtatttc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctc gaatttgaag cccatcgtcc gctgtatgac tgggtgttgg acaacatccc
     3541 cgcgccgcac gccacacgtc cgcgccaata cgagttttca cgcttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacca tttccggtat gcgccgacgc ggctacacgc ccgaagggct
     3721 gcgcctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaattccgcc ccacgcctga tggcggtgtt
     3841 gaacccgctc aaagtgaccc tgaccaactt tgaaaccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aggaaatggg cgagcgcgaa gtacctattt cacaaaccat
     3961 ctatattgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtggttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tccagaaggc cgtaaagtta aaggcgtgat tcattgggtt tccgccgaac acgccgccga
     4201 aatcaaagtc cgtctgtacg accgcctctt taccgtcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagacggc gaatacctgc cgttcaccga tttcctcaat ccggaatccg tcaaggaaat
     4321 caccgcctac gccgaacctg ccgccaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgtaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ttgccgtctg
     4501 aatattgttc ggacggcatt tcccctttgc ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctac
     4621 aaagacacca cctacgccat gatgcgcgag atggcaaaac gcggttggcg gctgtttcat
     4681 accttgagcg gggaattgtc ggttaaaaac ggtctggtgg ttgctgatgc gtctgctttt
     4741 gagtttctcg gcgcacaaga cgaccacgac aaaaactggt ttgccgccgc cgacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccacaca gcttctgacg ctggcggagc agcagggcgc gaaggtcttt
     4921 aacagcggac aggcgatgcg tgatttcaat gaaaaactgg cgattttgaa cttcagccgc
     4981 tttaccgcgc ccacgctggt aacgacccgt tccgccgatg tccgcgcatt tttgaaagaa
     5041 cacggcgaca tcatcattaa accgctggac ggtatgggcg gtatgggtat tttccgcctg
     5101 accgaaaaag atcccaacat cggcagcatc ctcgaaaccc tgatgcggct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtatcc cgcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg acgcggcgtg gcgcaggaat tggacggacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgctggc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatt
     5461 atgaaacaaa aaggtttcga cgtggcggca atgtttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgccgt ttgaaaggct tttgcttcac aaccgtttgg tttgttcggg
     5581 caggcagggt tttccctcag ccggcaggga tgcggttaat gccgtacgga caccccgccg
     5641 ccccgttttc agacggtata tatagtggat taacaaaaat caggacaagg cggcgaagct
     5701 gcagacagta caaatagtac ggaaccgatt cactcggtgc ttcagcacct tagagaatcg
     5761 ttctctttga gctaaggcgg ggcaacgccg tacaggtttt tgttaatcca ctatattgag
     5821 aacattttga aaggataccg atggaacctt cctcctacgc ggcagaaaaa aaaggcaaag
     5881 gcggcatcag gcgcgtcatt aacgcattcg gctattcgat agacggcatc gccgccgcct
     5941 accgttacga agcggcattc cgtcaggttt tgtggctgaa cgtactgctg gtgtgcgcgg
     6001 catttttttg ggtttccgaa accgccgtcc gcctgccgtt gattatcgcg tcttttgtgt
     6061 cggtcattgt cgaactgttc aacaccgccg tcgaagctgc cgtcgatcat acttcgaccg
     6121 aaaaacacga gctggccaaa cgcgccaaag acgcaggttc tgccgcacaa ttgttcgcga
     6181 tgctgatgtt ggcggcggtt tggctgtctg ccctgttcgg gtaaaacgct tgcagcagga
     6241 tttgtaatcc tttaggatta gtatgtggta tttattattc acttaatcca ctataagatt
     6301 aagcacattc cccgtctgat attataagga gcagaaagat gaaaaaatta ttggcggccg
     6361 tgatgatggc aggtttggca ggcgcggttt ccgccgccgg agtccacgtt gaggacggct
     6421 gggcgcgcac caccgtcgaa ggtatgaaaa taggcggcgc gttcatgaaa atccacaacg
     6481 acgaagccaa acaagacttt ttgctcggcg gaagcagccc cgttgccgac cgcgtcgaag
     6541 tgcataccca catcaacgac aacggcgtga tgcggatgcg cgaagtcaaa ggcggcgtgc
     6601 ctttggaggc gaaatccgtt accgaactca agcccggcag ctatcacgtg atgtttatgg
     6661 gtttgaaaaa acaattaaaa gagggcgaca agattcccgt taccctgaaa tttaaaaacg
     6721 ccaaagcgca aaccgtccaa ctggaagtca aaatcgcgcc gatgtcggca atggaccacg
     6781 gtcatcacca cggcgaagcg catcagcact aatctgctga aaatatttga aatgccgtct
     6841 gaaaaagccc gggctttcag acggcatttt tatgaccgcc tttaaaatgt gttaaaatct
     6901 gcctttaaaa accgccgttt ccaaccatat tctacgcatg aatacgacag caaacccttc
     6961 caatatcatc gtcgggctct ccggcggtgt cgattcttcc gtaaccgccg ccctgctcaa
     7021 gcagcagggt tatcaagtgc gcggtgtgtt catgcagaac tgggaagacg acgacaacga
     7081 cgaatattgc agcatcaaac aggattcgtt cgatgccatc gccgttgccg atattatcgg
     7141 catcgacatc gacatcgtta atttcgccgc gcaatataaa gacaaagtct ttgcttattt
     7201 tcttcaggaa tacagtgcgg ggcgcacgcc gaatcccgat gtgttgtgca acgccgaaat
     7261 caaattcaaa tgctttttgg attacgccgt agggcagggc gcggatacca tcgccaccgg
     7321 acactatgcg cgcaaagaag tccgcaacgg cgtgcattac ctgctcaaag gtttggatcg
     7381 aaacaaagac caaagctatt tcctctaccg cctcaagcct ttccaactcg agcgcgcgat
     7441 ttttccgttg ggcggtttgg aaaaacctga agtccgccgc cttgccgccg aatttaattt
     7501 gccgaccgcc gccaaaaaag acagcacggg catctgcttc atcggcgaac gcccgtttcg
     7561 cgagtttctg caaaaatacc tgccgaccga caacggtaaa atggttacgc ccgaagggaa
     7621 aaccgtcggc gaacacgtcg ggctgatgtt ctacacgctc ggacagcgca aaggtttggg
     7681 catcggcggc gcgggcgaac cgtggtttgt tgcggctaaa gatttgacga aaaacgaact
     7741 gattgtcgtg caaggacacg accatccgct gctctatacc cgcagccttg tgatgaacga
     7801 tttgagtttc acgctgcccg aacgcccgaa ggcaggacgc tatacctgca aaacgcgtta
     7861 ccgtatggcg gacgcgcctt gtgagttgcg ctatttggat gaagaaaccg ccgagctggt
     7921 gtttgacgaa ccgcaatggg cggttacgcc gggccagtct gccgtgctgt acgacggcga
     7981 tatctgtttg ggcggcggca tcatccaaac gaccgacaaa cccgtcatca tcacgcgata
     8041 aaggtaatgc cgtctgaaac ggttttcaga cggcattgtt ccgctcaatt ccactttaaa
     8101 gaccgatacc tatggaaaaa atctggttag acagttacga gaagggcgtc agtgccgaaa
     8161 tcgatatcac gcaatacaat tccatcagcg atgtattccg ccaaagcgtg gaaaaatttg
     8221 ccaggctgcc cgcttttcaa aatatgggca aaacgctgac ttatgccgaa accggcaaac
     8281 tggcgactga tttcgcctct tatctgcaaa acgtcctcaa gctgccgcgc ggcgaacgtg
     8341 ttgccattat gatgccgaac gtattgcaat acccgatagc ccttttcggc attttgcagg
     8401 caggtttggt ggcggtgaac accaatccgc tctatacgcc gcgcgagctg gagcatcagc
     8461 tgaaagacag cggcacgacc gccatcatcg ttttggaaaa tttcgccaac acgctggagc
     8521 tggtgctgcc gcgcacgcag atcaaacatg tcatcgtcgc ctccgtcggc gaaatgttcg
     8581 ggctgcttaa aggttcgctg atcaatttca tcatccgaaa aatcaagaaa atggttcccg
     8641 aataccgtat tcgggaaacc gtttcctttc agacggcatt gaaagagggg gcgaagcacg
     8701 ttttccaacc tgtcgcatta aaccgcgaag ataccgcgct gttgcaatac acgggcggca
     8761 cgacaggtgt tgccaaaggg gcggtgctga gtcacggcaa catctgtgcc aatatgcttc
     8821 aggcaaaaga atggattaaa aaccagttgc gcgagggcaa agaaaccgtt atcgctgccc
     8881 tgccgctgta ccatattttc gccttaaccg tgaatctgat gatttttgcc aatgccggct
     8941 caaaaatcat cctgattacc aacccgcgtg atatgaaagg ctttatcgga gaactgaaaa
     9001 aacagcggat taatgtattt atcggcgtaa acacactgtt taacgcgatg gttaaccgtc
     9061 ctgattttgc cgaagtcgat ttttcagaat tgcggctgac tttgggcggc ggtatggcga
     9121 cccaaaaagc cgttgccgaa aaatggaaaa aaattaccgg tacgcctatt gtcgaagcct
     9181 acggtctgac cgaagccagc ccaggcgtgt gctgcaaccc cttaaacatc gaatcataca
     9241 gcggcagcat cggtttgccc gtctcgtcca ccgaagtcga actgcgcgac gcaaacggca
     9301 aagaagtccc cgtcgggcag ccgggcgaat tgtgggtaaa aggccctcaa gtgatgcaag
     9361 gctactggaa ccgtcccgaa gaaaccgcta aagccataga cgcgcgcggt ttcttggaaa
     9421 ccggcgatat tgccgtcatg gacgaaaaag gctggttgaa gctggttgac cgtaagaaag
     9481 acctcattgt tgtttccgga ttcaatgttt atccgaacga aatcgaagaa gtcatcgcgc
     9541 atcacggcaa agtgatggaa gttgcctgta tcggcgttcc cgacgaaaaa accggcgagg
     9601 cactcaaagt attcgtcgtc agaaaagacc cgtctttgac gaaagaagaa cttatcgcct
     9661 tttgccgcac cgaattaacc gcatataaag taccgaaaga tatcgaattc cgcgacgagt
     9721 tgcccaagtc caacgtcggc aaaatcctgc gccgcgagtt gcggcaaagt accgggaaat
     9781 aaagaaaaga tgccgtctga aaacagccgt ccaccgttca gacggcatcc gtccgtttgc
     9841 cagaaccgcg cgcttcacgt taaaatcacg cattccaata tgggtattcc atcatgacca
     9901 aattcatttt cgtaaccggc ggcgttgtct cctcactggg taaaggtatc gccgccgctt
     9961 ctattgccgc catcctcgaa tcgcgcggct tgaacgttac catgctcaag ctcgatcctt
    10021 atatcaacgt cgatcccggc acgatgagcc cgttccaaca cggcgaagtg ttcgtaaccg
    10081 acgacggcgc ggaaaccgac ctcgacttgg gacactacga acgctttatc gattccacga
    10141 tgacccgccg caacagcttc agcacggggc aggtgtacga aaacgtcatc gccaaagaac
    10201 gacggggcga ctatttgggc ggcacggttc aagtcattcc gcacattacc gacgaaatca
    10261 aacgccgcat ccacgaaggc gcggcaggtt acgatgtggc gattgtcgaa atcggcggta
    10321 cagtcggcga tatcgaatct ttgccattct tggaagccat ccgccagatg cgaagccagt
    10381 tgggacgcaa caacaccctg ttcgcccact tgagctacgt cccctacatc gccgctgcag
    10441 gcgaaatcaa aaccaagccg acccagcaca ccgttaaaga aatgttgagc atcggtttgc
    10501 aacccgacat cctgatttgc cgtatggaca ggacaatgcc tgcggacgaa cgccgcaaaa
    10561 tcgccttgtt ctgcaacgtg gaagaacgcg cgattgtcgg cagctacgat gtggacagca
    10621 tctacgaatg ccccgaaatg ctgcacgacc aaggcatcga caacatcatt accgagcagt
    10681 tgcagcttaa tgtgcagcag gcggatttga ccgcgtggaa aaaaatcgtc cacgccatcc
    10741 aaaacccgaa acacaccgtc aaaatcgcca tggtcggcaa atacgtcgat ttgaccgagt
    10801 cctacaaatc gctgatcgaa gccttgaaac acgcaggcat tcacaccgaa accgacgtac
    10861 aaattacctt tgttgacagc gaaagcatcg agaaaaacaa cggcgacgtt tccatgctta
    10921 aagacatgga tgccatcctc gttcccggcg gttttggttc gcgcggcgtg gaaggcaaaa
    10981 tcgccgctgt gcgctatgcc cgcgaaaaca acgtgccata cttgggcatc tgcctcggta
    11041 tgcagattgc gctgatcgaa tacgcccgcg acgtggcagg tttgaaaggt gcgaattcca
    11101 ccgagttcga cctcaaatgc gccgcccccg tcgttgccct gattgacgaa tggcaaactg
    11161 ccgacggcag cgtcgaaacc cgcgacgaat ccgccgattt gggcggcacc atgcgtttgg
    11221 gcgcgcaaga agtcgaattg aaagcaggca gcctcgccgc caaaatctac ggcagcgaac
    11281 acatccgcga acgccaccgc caccgctacg aagtcaacaa caactacgtt cctacgctgg
    11341 aacaggcagg tttggtcatc ggcggcgtat ccgccggacg cgaacgcttg gtcgaaacca
    11401 tcgaactgcc gaaccatcct tggttcttcg cctgccagtt ccaccccgag tttacctcca
    11461 atccgcgcaa agggcatcct ttgttcaccg cgtttgtcaa agctgcgttg aacaataaaa
    11521 aagcctgata aagcgttact tgatgatcaa aatgccgtct gaaagcctga atgaagactt
    11581 tcagacggca tttttgcaaa tcggggatca taaaagccat caatttaatt tttatggttt
    11641 tatctaatat tgaccaactg aatattgtta agcggggcgt gaaactcaag ttgagacctt
    11701 tgcaaaattc cccaaaatcc cctaaattcc caccaagaca tttaggagca ccttcttccg
//
LOCUS       DAWEWP010000052        11611 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWEWP010000052 DAWEWP010000000
VERSION     DAWEWP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.052"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(227..1270)
                     /locus_tag="WGN53_001014"
     CDS             complement(227..1270)
                     /locus_tag="WGN53_001014"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ0140020.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIWDIPTWEHWYGMPHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(1267..1548)
                     /locus_tag="WGN53_001015"
     CDS             complement(1267..1548)
                     /locus_tag="WGN53_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140021.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            1857..2957
                     /locus_tag="WGN53_001016"
     CDS             1857..2957
                     /locus_tag="WGN53_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0140022.1"
                     /translation="MYQKPKLFKGAVRNLEAASWKDINEIYQRADPTAPLFYLRKKGA
                     IVPKEEYVESFDALGKTRYRFIKSVIYEHMKNGASLVYNHINNEPFSDHISRQVARLA
                     GAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQLTAPDFPMPLYMQQTKDTD
                     ISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHLAVGTFPPNGYNYLEWLMKK
                     FPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEAFSEDFLGKERTDTAFHLE
                     QFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISVDELGKKVLEHIGKNEPLL
                     LKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3186..3539
                     /locus_tag="WGN53_001017"
     CDS             3186..3539
                     /locus_tag="WGN53_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140023.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRDWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3600..4226
                     /locus_tag="WGN53_001018"
     CDS             3600..4226
                     /locus_tag="WGN53_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ0140024.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTAEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQKLH
                     ISQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRATNLFIS"
     gene            4178..4378
                     /locus_tag="WGN53_001019"
     CDS             4178..4378
                     /locus_tag="WGN53_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140025.1"
                     /translation="MVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADI
                     LHEKRRLYEWVLELIYSMSGKL"
     gene            4687..4989
                     /locus_tag="WGN53_001020"
     CDS             4687..4989
                     /locus_tag="WGN53_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140026.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5273..5512
                     /locus_tag="WGN53_001021"
     CDS             5273..5512
                     /locus_tag="WGN53_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0140027.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            5573..7315
                     /gene="hrpB"
                     /locus_tag="WGN53_001022"
     CDS             5573..7315
                     /gene="hrpB"
                     /locus_tag="WGN53_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ0140028.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISTFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGTQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            7424..>11611
                     /locus_tag="WGN53_001023"
     CDS             7424..>11611
                     /locus_tag="WGN53_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0140029.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTGVLARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTTALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPA
                     TTVLNAGHNLVIESKTNVNNAKGSANLSAGGRTTINDATIQAGSSVYSSTKGDTELGE
                     NTRIIAENVTVLSNGSIGSAAVIEAKDTAHIESGKPLSLETSTVASNIRLNNGNIKGG
                     KQVVLMADGDIQAKASNLNASGNLYIHAGKDLDLNADKDLSTQSISLRADNTALISSN
                     GNTLTAEKNLDIQAGSLSVRQSNLQSSGGNVQMSATKGNISLNQSWINASQNIDTAAL
                     QGNIISDGLTAVAEVGRVSLLANGNVDFNGLNTLIAEGDINAGSVGKGRLKMDNTDIY
                     ASAGDVKLVAGGQLDLGNGTVNGGHISLDSNKGSMVVQDVHLNARASLKVDADQTLTI
                     NNSKLNSGHNTQINTNHGHMTLNQLDAHSRRHMSISAQGKGKGKDSGQILQNDQQNSK
                     STLAADGVLSLNSSALQVLDNTTLRGGAINIKAGGGIIKRGYIDWETQDTATMRSAEL
                     KPLSGMMSIESGGDNPLTVEPGNRIVSAGDLAVNHNGTFQISARAGNNGNPSAQTASV
                     SAKGNIGIVAGEVDIDAANIAAGKDLALVATKGNISLNSIRNTFSNYQLKTDKHNITQ
                     QLTDVEQELSKLTSDPKYRKAQDLPQMLRRKYKRRDKVFGDSEARLRGLRAEINAADE
                     AWAELQSPVKALLERKQLLQQALLTVSQPGSGHENQGSTLSGQNIKLLAAGGIRIQGS
                     KVAATQQANIQAAGFLPAPAAEELQEGRLQSAIDISGVFDTFEYGQQGSDKYGYAIFS
                     RPSEISGKTGVTLSAPNANENSRISLSAANIEAENGKIKLQSYGDQSYYAGQGELYTL
                     ERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQGIDIKSGGNIDAYATAFDAPKG
                     SINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYSKAHDTTTQVMKTALPSRVVAE
                     SANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARADAKIILEGIKSSIHTETVSSSK
                     STLWQKQAGRGSNIETLQ"
BASE COUNT         3319 a         2648 c         2845 g         2799 t
ORIGIN      
        1 aatgactgaa accggagaaa tgacgaaatt gaagcgtgtg gaaatgtgtc ggaaatcgct
       61 gaagccggat aaactagatt cccgcttttg cgggaatgac gggattttag gtttttgttt
      121 tcgtgggaat ggatgggccc aaagccgtcc gaaccaaaaa aaaccgccgc tttcagacgg
      181 catcaaaaaa ccgcaggcac gatgcctgcg gtttgccgtt tgcgcttcag gggagcaggg
      241 ggattgcgcc caagccgtgt gtttctttca gtccgaacat gatgttcata ttttgcaccg
      301 cctgacccgc cgcgcctttg acgaggttgt cgatgacgga aaggacgacc cacacatcgg
      361 attgcgccgc ctgttggatg ctgatgcggc agaggtttgc gccgcgcacg ctgcgggttt
      421 ctggtgtcga accggcgggc aggatgtcca cgaacaggct gtcgcggtaa tagtcgcgca
      481 ggatggtttc ggggcagatg ccgtctgaaa ggtggaggta aacggtggcg tgcataccgc
      541 gtatcatcgg cgcgaggtgc ggcgtgaaca cgaatccttc ggcgatgccg tcctgaagcc
      601 cggcgatggt ctgcctgatt tcgggcaggt ggcggtgtcc ggctatgccg taggctttga
      661 agttgtcgcc gacttcgcac aacagcgaac cgacattgcc tttcctgccc gcgccggaca
      721 cgccggattt gcagtcggca atcagcggca taccgggctt cagacggcat tgccgcaaca
      781 gcggcacgag cggcagggat acgcaggtcg ggtagcagcc ggggttggcg acgaggcgcg
      841 cctgtgcgac ggcttcgcgg ttgagttcgc tcaatccgta cacggcttgg gaaacgaggc
      901 cgggggcggc gtggggcatg ccgtaccagt gttcccaggt cggaatgtcc catatgcgga
      961 agtcggcgga aaggtcgatg acgcgcacgc cctgttcaat caggcgcggc gcgtctttca
     1021 tggcgatgcc gttgggcgtg gcgaagaaga cgatgtcgca ttgttccaaa cctgcctcgt
     1081 cgggcgtttg gaaggcgagg ccgtacacgc cgcgcaaact cggaaagtaa tcggcaactg
     1141 cggttcccgc ttcgctgcgg ctggttacgg cggcgacttc gacatcggga tgggcggcaa
     1201 gcaggcgcag cagttccacg ccggtgtagc ccgtcgcgcc gacaatgccg actttgattt
     1261 ttttactcat ggtttttcct ttgtgtggtt ggcgggtatg ccgtccgaac gctgtccgac
     1321 ggggttttga ttggcggcgg aaggacggta cacaaagcgt ttgaaaagcg tgtctaattt
     1381 aatggcatcc gcctctgttt ttaaaaccca gccctgccgt ccggaataaa cataaccgta
     1441 ggcgcgaaag ccggcaggat atttcggtgc ctgccccaaa cccaaaccgt atgcggcgaa
     1501 agcggtggtc gtggagaagg aaagcaggag gaggacgagg gttttcataa ggttccaaac
     1561 cgaacggggc gatgaagccc gattatagca aaaggtcgtc tgaaaaccgt tttcagacga
     1621 ccttttattt aattacaata aaaatgttaa caacaaaaaa caaaccgctt ttttccgttt
     1681 ggagacaaat ttttaactaa aattgtcaac aattccttga cgcacacaca aaaaaaactt
     1741 taatatttgt aaatatatgt aacattataa attttaaata ttttctgagt aaggggaagt
     1801 aatgagcatc catctcgact tcggcattag tcctaaaacg ttccgacaga cttaatctgt
     1861 atcaaaagcc caagctcttt aaaggagcgg ttcggaatct cgaagccgca tcttggaaag
     1921 atatcaacga gatataccaa cgagcagacc caaccgcacc gctgttttat ctgcgtaaaa
     1981 aaggtgcaat tgttcctaaa gaagaatacg tcgaaagttt cgacgctttg ggcaaaactc
     2041 gctaccgttt tattaaatcc gttatctacg aacatatgaa gaatggtgcg tcgttagtct
     2101 ataaccatat taacaacgag ccgttttcag atcatatttc ccgtcaagtc gcccgccttg
     2161 ccggcgcaca tactattgtt agtgggtatc ttgcttttgg cagcgacgaa tcttataaaa
     2221 accattggga cacccgcgat gtgtatgcca tccagctttt cggcaagaaa cgttggcaac
     2281 ttactgcccc tgatttccct atgccattgt atatgcaaca gactaaagat actgatattt
     2341 ccattcctga acatatcgat atggatatta tccttgaagc aggtgatgtc ctctacatcc
     2401 cgcgcggttg gtggcacaga cctatcccgc tcggctgtga aaccttccac ctcgctgtcg
     2461 gtacctttcc gcccaacggc tataattacc tcgagtggct aatgaagaaa ttccccacca
     2521 tagaaagtct gcgccacagt ttctcagact gggagcaaga taggacgcgt atcaacgata
     2581 ctgccgcaca aattgctgcc atgattgccg accccgtcaa ttatgaagcc ttcagtgaag
     2641 actttctcgg caaagaacgt accgataccg cttttcatct cgaacagttc gcgaatccca
     2701 acgctactcc gctttcagac gacgtcaggt tgagattaaa tgccaataat ttggatacgt
     2761 tggaaaaggg atatttgatt gggaatggga tgaagataag cgtagatgag ttggggaaaa
     2821 aagttttaga acacatcggt aagaatgaac cgttattgtt gaaaaatcta ctggttaact
     2881 tcaatcaggg aaaatatgaa gaagttagga agttgattta tcagttgata gagttagatt
     2941 ttctggaaat tttgtgaggg attctatgaa aaactggaag caatttacat ttttcgtaat
     3001 attagtaata gcttgttatc aagtgctata ttttttatca gatatgtttc ttctcgacta
     3061 cattaacaaa tatagttgga atttgaattt tattcagggt actttgaatt ttttttcaat
     3121 atatctgcca tatgtttttg ttagccgaat ttttaggaat accaaccaag aaaaggagta
     3181 taaaaatgat tgaacttcaa cttcatgaat tgaagctggt ttcaggggga ggtcctgtaa
     3241 cagacaatat agctggaaat gtagctaatg ctgccacaac caaaggaggt cccacatggg
     3301 gggatttggt tgcaatacct gctgccgcag cggcagttta ttatctgccg aaaaatgctt
     3361 acggtgctgc tggtgcaaat ggtgtataca atgtgactcg tgattgggta aatgatgccg
     3421 ttaatgcacc tccttataac ggaagaccaa tctttgagat tgaacatgga ttaactgctc
     3481 ccgcaacaaa agcagataaa tcaggaaacg gctacactga cggtacagat tactgctgat
     3541 atttctcatt gttcagacga tctctagggg tcgtctgaaa ctttcttaac ttcaatttta
     3601 tgaatagacc caagcaaccc ttcttccgtc ccgaagtcgc cgttgcccgc caaaccagcc
     3661 tgacgggtaa agtgattctg acacgaccgt tgtcattttc cctatggacg acatttgcat
     3721 cgatatctgc gttattgatt atcctgtttt tgatatttgg taactatacg cgaaagacaa
     3781 cagcggaggg acaaatttta cctgcatcag gcgtaatcag ggtgtatgca ccggatacgg
     3841 ggacaattac agcgaaattc gtggaagatg gagaaaaggt taaggctggc gacaagctat
     3901 ttgcgctttc gacctcacgt ttcggcgcag gaggtagcgt gcagcagcag ttgaaaacgg
     3961 aggcagtttt gaagaaaacg ttggcagaac aggaactggg tcgtctgaag ctgatacacg
     4021 ggaatgaaac gcgcagcctt aaagcaactg tcgaacgttt ggaaaaccag aaactccata
     4081 tttcgcaaca gatagacggt cagaaaaggc gcattagact tgcggaagat cagtggcaaa
     4141 aacggcactg ggcagacagg aattgtcggg attgggcatg gtatcctccg atttggcgaa
     4201 gagcaacgaa cctgtttatc tcgtgaaaat aaaacccgac aaaccaacca tcactgcata
     4261 cggtgaggaa aaaccgctgc aaatcggcat gacgttggaa gcagacatcc tgcacgagaa
     4321 acggcggctg tacgaatggg tattggagct gatttatagt atgtcgggca aactgtaaga
     4381 cttgaggctg aaagataaag aagttgtctg aaaccttttc agacgaccag aagacaataa
     4441 aaaacacaaa cttcaaaact gcctgaaacc atcccctttt acaggcactc cgtttgtaaa
     4501 agcaaggata gggcatctgc ccttttgatt gtttgattaa cgatacaagg agtttcaaaa
     4561 tgagagtttt atagtggatt aacaaaaacc agtacagcgt tgcctcgcct tgccgtacca
     4621 tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatatgtgtt
     4681 catgaaatga cttgggtcgg aggctcaggt aatgcgcaac aaagctcata ttattgcgaa
     4741 atttgcgaat ctgcagggct taacgatacg ggaaatcctg ataaatcttt aggattgcca
     4801 aacaatacgt tcagtaatcc gcctggttgg ggagctacaa tcggagcttt agcaggtagc
     4861 cgcataggga tgcctgaatt tggtacgttt gcgagccatg ccattgaaaa tttcgactgg
     4921 tcatggtatc gacgttatag ggaaattgcc gaaacgattg aacgagaata ttcaggcggt
     4981 ttgccttaat agttgaggag gtcatgatgt ttgccaaaca ttatcaattc atcgcactcg
     5041 gcatcatgct gcttctttat atgttgattc tctatacgac cgatttttcc aatctgacgt
     5101 attggatgct gttttttatc tgttttatta caggaaaaat attagctcgt ttgttagaga
     5161 aaagctttaa ataaaatagc agctagtcgc aaaaggtcgt ctgaaacctt ttcaggcggc
     5221 ctttctaaaa tacatccaac ttcctaatcc ctatttttca aaaaggaaat ctatgcccca
     5281 tctgcaaaac ctgtctttgg gcttaaagaa aaagctgcct gttatcctgc aaacagaaat
     5341 atcagaatgc ggcttggcat gtctggcggc tgtggcggga tttcatggtt tccatacgaa
     5401 tttacgcgca ctgcgttcaa aatactgtcc gagacctttg caaaattccc caaaatcccc
     5461 taaatgtctt ggtgggaatt ttggggaatt ttgcaaaggt ctcattctat aactgtaaat
     5521 acttttaaat ttatgacaaa atagtaaata ttgctaaaat aatattgatg tcatgaaatt
     5581 ttttcctgct ccatgtctgt tggttatcct ggctgtcata ccccttaaaa ccttagctgc
     5641 cgatgaaaac gatgcagaac ttatccgttc catgcagcgt cagcagcaca tagatgctga
     5701 attgttaact gatgcaaatg tccgtttcga gcaaccattg gagaagaaca attatgtcct
     5761 gagtgaagat gaaacaccgt gtactcgggt aaattacatt agtttagatg ataagacggc
     5821 gcgcaaattt tcttttcttc cttctgtgct catgaaagaa acagctttta aaactgggat
     5881 gtgtttaggt tccaataatt tgagcaggct acaaaaagct gcgcaacaga tactgatcgt
     5941 gcgtggctac ctcacttccc aagctattat ccaaccacag aatatggatt cgggaattct
     6001 gaaattacgg gtatcagcag gcgaaatagg ggatatccgc tatgaagaaa aacgggatgg
     6061 gaagtctgcc gaaggcagta ttagtacatt caataacaaa tttcccttat ataggaacaa
     6121 aattctcaat cttcgcgatg tagagcaggg cttggaaaac ctgcgtcgtt tgtcgagtgt
     6181 taaaacagat attcagatta taccgtccga agaagaaggc aaaagcgatt tacagatcaa
     6241 atggcagcag aataaaccca tacggttcag tatcggtata gatgatgcgg gcggcaaaac
     6301 gaccggcaaa tatcaaggaa atgtcgcttt atcgttcgat aaccctttgg gcttaagcga
     6361 tttgttttat gtttcatatg gacgcggttt ggcgcacaaa acggacttga ctgatgccac
     6421 cggtacggaa actgcaagcg gatccagaag ttacagcgtg cattattcgg tgcccgtaaa
     6481 aaaatggctg ttttctttta atcacaatgg acatcgttac cacgaagcaa ccgaaggcta
     6541 ttccgtcaat tacgattaca acggcaaaca atatcagagc agcctggccg ccgagcgcat
     6601 gctttggcgt aacagacttc ataaaacttc agtcggaatg aaattatgga cacgccaaac
     6661 ctataaatac atcgacgatg ccgaaatcga agtgcaacgc cgccgctctg caggctggga
     6721 agccgaattg cgccaccgtg cttacctcaa ccgttggcag cttgacggca agttgtctta
     6781 caaacgcggg accggcatgc gccaaagtat gcctgcaccg gaagaaaacg gcggcgatat
     6841 tcttccaggt acatctcgta tgaaaatcat tactgccggt ttggacgcag ccgccccatt
     6901 tattttaggc aaacagcagt ttttctacgc aaccgccatt caagctcaat ggaacaaaac
     6961 gccgttggtt gcccaagata aattgtcaat cggcagccgc tacaccgttc gcggatttga
     7021 tggggagcag agtcttttcg gagagcgagg tttctactgg cagaatactt taacttggta
     7081 ttttcatccg aaccatcagt tctatctcgg tgcggactat ggccgcgtat ctggcgaaag
     7141 tgcacaatat gtatcgggca cgcagctgat gggtgcagtg gtcggcttca gaggagggca
     7201 taaagtaggc ggtatgtttg cttatgatct gtttgccggc aagccgcttc ataaacccaa
     7261 aggctttcag acgaccaaca ccgtttacgg cttcaacttg aattacagtt tctaacctct
     7321 gaatttttta ctgatattta gacggtcttt ccttatcctc agaccgtcaa actttaccta
     7381 cgtacttggc gcgcagtacg ttcatcttca aaatggaata gacatgaata aaggtttaca
     7441 tcgcattatc tttagtaaaa agcacagcac catggttgca gtagccgaaa ctgccaacag
     7501 ccagggcaaa ggtaaacagg caggcagttc ggtttctgtt tcactgaaaa cttcaggcga
     7561 cctttgcggc aaactcaaaa ccacccttaa aaccttggtc tgctctttgg tttccctgag
     7621 tatggtattg cctgcccatg cccaaattac caccgacaaa tcagcaccta aaaaccagca
     7681 ggtcgttatc cttaaaacca acactggtgc ccccttggtg aatatccaaa ctccgaatgg
     7741 acgcggattg agccacaacc gctatacgca gtttgatgtc gacgctaaag gtgctgtact
     7801 gaacaacgat cgcaacaaca acccgtttct ggccaaaggc agtgcgcagt tgattttgaa
     7861 cgaggtgcgc ggtgcggcca gcaaactcaa tggcatcgtt accgtaggtg gtcaaaaggc
     7921 cgatgtgatt atcgccaacc ccaacggcat taccgtgaac ggcggcggct ttaaaaacgt
     7981 cggccgcggt atcttaacta ccggtgcgcc ccaaatcggc aaagacggtg cactgacagg
     8041 atttgatgtg cgtcaaggca cattgaccgt aggagcagca ggttggaatg ataaaggcgg
     8101 agccgactac accggggtac ttgctcgtgc agttgctttg caggggaaat tacagggtaa
     8161 aaacctggcg gtttctaccg gtcctcagaa agtagattac gccagcggcg aaatcagtgc
     8221 aggtacggca gcgggtacga aaccgactat tgcccttgat actaccgcac tgggcggtat
     8281 gtacgcagac agcatcacac tgattgccaa tgaaaaaggc gtaggcgtca aaaatgccgg
     8341 cacactcgaa gcggccaagc aattgattgt gacttcgtca ggccgcattg aaaacagcgg
     8401 ccgcatcgcc accactgccg acggcaccga agcttcaccg acttatctct ccatcgaaac
     8461 caccgaaaaa ggagcggcag gcacatttat ctccaatggt ggtcggatcg agagcaaagg
     8521 cttattggtt attgagacgg gagaagatat cagcttgcgt aacggagccg tggtgcagaa
     8581 taacggcagt cgcccagcta ccacggtatt aaatgctggt cataatttgg tgattgagag
     8641 taaaactaat gtgaacaatg ccaaaggctc ggctaatctg tcggccggcg gtcgtactac
     8701 gatcaatgat gctactattc aagcgggcag ttccgtgtac agctccacca aaggcgatac
     8761 tgaattgggt gaaaataccc gtattattgc tgaaaacgta accgtattgt ccaacggcag
     8821 tattggcagt gctgctgtaa ttgaggctaa agacactgca cacattgaat cgggcaaacc
     8881 gctttcttta gaaacctcga ccgttgcctc caacatccgt ttgaacaacg gtaacattaa
     8941 aggcggaaag caggtggtac tgatggctga tggcgacatt caggcaaaag cctccaatct
     9001 gaatgcatcc ggcaatctgt atattcatgc aggtaaggat ctggatttga atgccgataa
     9061 agacttatca acacaaagta tcagcctgag ggcagacaac accgccctca tcagtagcaa
     9121 cggtaacacc ttgactgcag aaaagaatct ggatattcag gcaggtagct tgagtgtgcg
     9181 tcaaagtaat ctgcaatcca gtggcggtaa tgtgcagatg agcgcgacta aaggcaacat
     9241 cagcttgaat cagagttgga taaatgccag tcagaatatt gatacggcag cacttcaagg
     9301 caatattatt tcagacggtc tgactgctgt tgccgaagtt ggacgcgtat ctcttcttgc
     9361 caacggcaat gttgatttca acggtctaaa taccttgatt gcggaaggag acattaatgc
     9421 tggttcagtc ggtaaaggcc gtctgaaaat ggacaatacc gatatttatg cttctgcagg
     9481 cgatgtgaaa ctggttgccg gaggtcaatt agatttaggc aacggcaccg ttaacggcgg
     9541 tcatatcagc ttagacagca ataaaggcag catggtggtg caggatgtac acctgaatgc
     9601 ccgcgcatca ctgaaagtgg atgcggatca aactttaacc attaataaca gcaagctcaa
     9661 ttccggtcac aatacccaga ttaatacaaa tcatggtcat atgacgctta atcagcttga
     9721 tgctcattca cgtcgtcata tgagtatcag tgcacagggt aaaggcaaag gaaaagacag
     9781 cggtcaaatt ttacaaaacg accagcaaaa cagtaaaagt actttggcgg cagatggtgt
     9841 attgtcattg aacagcagcg cattacaggt tttggacaat actaccctgc gcggtggcgc
     9901 gataaacatc aaagctggcg gaggcatcat caaacggggc tatatcgatt gggaaaccca
     9961 agatacggct actatgcgtt cagcggaact gaaaccactg tctggtatga tgtcgataga
    10021 atcgggcggt gataaccctt taaccgttga acccggtaac cgtatcgttt ctgcaggtga
    10081 tttggccgtg aaccacaacg gcacattcca aatcagtgcc agagcaggaa ataacggcaa
    10141 tccgagtgcg caaacagcca gcgtttcagc taaaggcaat atcgggattg tggcgggaga
    10201 ggtggatatt gatgcagcaa atattgccgc agggaaagat ttggctttag tggcaactaa
    10261 agggaatatt tcactaaaca gtattaggaa tacgtttagt aactatcaat tgaaaacaga
    10321 taaacacaat atcacacagc aacttactga tgtagagcag gagcttagta agttaacgag
    10381 cgatcctaaa tatcgtaaag cacaggatct tccacaaatg ttgaggcgta aatataagag
    10441 gcgtgacaaa gtattcggtg atagtgaggc cagattacgt ggtttacgag cagagataaa
    10501 tgctgctgat gaggcttggg cagaactcca atccccagta aaagcgttac ttgaacgtaa
    10561 acaattgtta caacaagccc ttttaacagt ttcccaaccg ggtagtggtc acgaaaacca
    10621 aggtagcacc cttagcggtc aaaatatcaa actgctcgct gcaggtggta tccgtatcca
    10681 gggctccaaa gttgctgcca cccaacaggc aaatatccaa gctgcaggat ttttacctgc
    10741 accagccgca gaggaactgc aagaaggtcg acttcaatca gccattgaca tcagcggtgt
    10801 atttgatact tttgaatatg gtcagcaagg cagcgataaa tacggctatg ccattttcag
    10861 caggccatct gaaatttcag gaaaaacagg tgttaccctc tctgcaccaa atgccaatga
    10921 gaacagccgc atcagtctga gtgcggcaaa catcgaagct gaaaacggca aaatcaaact
    10981 gcaatcctac ggcgaccaat cctattacgc cgggcagggc gaactctata ccttagaacg
    11041 ccgcagctac aaaaccggca aatggtacaa ccgcaaacac attaccgaag tcaaagaaca
    11101 caaaaacgcc aagcccgacg cagtaaacct cagcgcatcc caaggcatcg acatcaaatc
    11161 tggtggcaac atcgacgcct acgccaccgc attcgatgcc cccaaaggca gcattaacat
    11221 cgaagccggg cggaaattga cactctatgc cgtagaagag ctcaactacg acaaacttga
    11281 cagccaaaaa aggcgcagat ttctcggcat cagctacagc aaagcacacg acaccaccac
    11341 ccaagtcatg aaaaccgcgc tgccctcaag ggtagttgca gaatcagcca acctccaatc
    11401 gggctgggat accaaactgc aaggcacaca gtttgaaacc acactgggtg gcgcaaccat
    11461 acgcgcaggc gtaggcgagc aggcacgggc agatgccaag attatcctcg aagggatcaa
    11521 aagcagcatc cacacagaaa ccgtgagcag cagcaaatct actctatggc aaaaacaggc
    11581 aggacggggc agtaacatcg aaaccttgca a
//
LOCUS       DAWEWP010000053        11533 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWEWP010000053 DAWEWP010000000
VERSION     DAWEWP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.053"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            121..543
                     /locus_tag="WGN53_001024"
     CDS             121..543
                     /locus_tag="WGN53_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140030.1"
                     /translation="MKKTFLSAVLLLSAATQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGWGRGRWATMKFQNGEFMYTVDAGFDSV
                     THAVGSGVLVERRGKEIARFDCTSKTAQANFDDDDFAW"
     gene            582..1322
                     /locus_tag="WGN53_001025"
     CDS             582..1322
                     /locus_tag="WGN53_001025"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ0140031.1"
                     /translation="MLIVRPSGRAKDDVETCRRAEWQAEVLSPIEIETDKASLKRLPE
                     MYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRALERCLGRTVIAPDDGN
                     DSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFRRHKPLNF
                     QNFQTENIAAAYITSAELVQSLFAQVPPQFSRFFKSLLYFTHHPRIAEALKREGVCSV
                     ETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            1337..2671
                     /locus_tag="WGN53_001026"
     CDS             1337..2671
                     /locus_tag="WGN53_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0140032.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            2668..3885
                     /locus_tag="WGN53_001027"
     CDS             2668..3885
                     /locus_tag="WGN53_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ0140033.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            3960..5024
                     /locus_tag="WGN53_001028"
     CDS             3960..5024
                     /locus_tag="WGN53_001028"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ0140034.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     MQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRAEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            5202..6581
                     /gene="radA"
                     /locus_tag="WGN53_001029"
     CDS             5202..6581
                     /gene="radA"
                     /locus_tag="WGN53_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ0140035.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSSVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(6856..7335)
                     /locus_tag="WGN53_001030"
     CDS             complement(6856..7335)
                     /locus_tag="WGN53_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ0140036.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAAHRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(7467..7826)
                     /locus_tag="WGN53_001031"
     CDS             complement(7467..7826)
                     /locus_tag="WGN53_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ0140037.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPLAAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAKAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(7865..11479)
                     /gene="recB"
                     /locus_tag="WGN53_001032"
     CDS             complement(7865..11479)
                     /gene="recB"
                     /locus_tag="WGN53_001032"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ0140038.1"
                     /translation="MSAPIQAFDPLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQTDLPQARDAALASWQNICGNLAELKETFWRIHPLLNGNSYRKNSFAELF
                     DALEQKALSPDLPFLEAKLHERLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFRRD
                     LDALEEAEEATIIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAECRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRQTYDWEKDGIAMLKRNWRRLTDNAPAGTDFAFTEDAPPPAAYRGNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICVIDYKSNHLGADASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         2521 a         3049 c         3417 g         2546 t
ORIGIN      
        1 tgccgcccta tgtgttgccc taatttggaa ggggttacac ccttttcaaa taaaatctga
       61 tgctgctgcc acgaaggacg gatgtccaag tggcggggtt ttaaccatta aggaaatacg
      121 atgaaaaaaa cgttcctttc tgccgtattg cttctgtcag ctgcgaccca aaccgtgtgg
      181 gcagatacgg tgttttcctg taaaacggac aacaacaaat atatagaagt tcaaaaaatt
      241 aaccgcaatc tttacgaata ttcgttcggc agtgcggcaa aaaaagaaat tgccatacgc
      301 aacagcaaag ctgatctgtt ggggcgttcc gacaggtggc aaggctgggg cagaggccgt
      361 tgggcgacga tgaaattcca aaacggcgaa tttatgtaca ccgtagatgc aggcttcgat
      421 tccgtgaccc atgcagtagg cagcggtgtc cttgtggagc gtaggggcaa ggaaatcgca
      481 cggttcgact gtacgtcgaa aaccgcgcag gcgaatttcg acgatgacga ttttgcctgg
      541 taatcggggc ggataaggcg atagaaacag tgaaacccat catgctgatt gtccgaccgt
      601 cgggcagggc gaaggacgat gtggaaacct gccgtcgtgc cgaatggcag gcggaagtgt
      661 tgagtccgat tgaaatcgaa accgacaaag caagcctgaa acggctgccg gaaatgtatg
      721 cccgtgcgga tgccgtgttt tgggtcagtc cgaccgccgt tgaaaccgcc gtcccgtacc
      781 ttaacctttc agacggcata aagatgcaca tttccgtagg gcagggcagc cgccgtgcat
      841 tggaacgctg tttgggcaga acggtcatcg cgcctgatga cggcaacgac agcgaggcgg
      901 ttttgcgcct gccggtttgg aacagtctgc ccgaaggtgc gcgcgtattg tttgtgcgcg
      961 gacacggcgg gcgggatttt ctgatgaatg ccttgcagga gaaaggtttt cggacggagg
     1021 tggcagaagt ctatttcaga cggcataaac ctttgaactt tcaaaatttc caaaccgaaa
     1081 atattgccgc cgcctatatt acgtccgcag aactcgtgca gtcgctgttt gcacaggttc
     1141 cgccgcaatt ttcccgattc ttcaaatcct tgctatactt tacccatcat ccgcgcattg
     1201 cggaggcatt gaagcgcgaa ggcgtgtgtt cggtcgaaac cgtccctacg ctggaagccg
     1261 cgctttccca ttcttccatt tccgtttcag acggcatggt ctttcccgga acctcaaatt
     1321 aataaggagc aaaacggtgg gcgaacctga aaacaaatca tccgaacccg tacgcgagat
     1381 acaggcatca aaagaaatgc cgtctgaaac ctcttcccca cgcaaagaaa acgaaacaga
     1441 agtacacatt cctgccgctc cttttatcgt caaacagtcc ggcagcaacg ctttggcagt
     1501 ctgcgccctg gtattggcgg cattgggttt gggtacaagt ggttttttgt ttgtccaggg
     1561 acagaatgtc ttgaaaaacc aagagctggc attcaaccaa aaaatcgaca aagccgcctt
     1621 gggcgagtcg gaaaacgccg ccctgttgaa agacaacctc aaccggcaag ccgccataca
     1681 atcagagctc gaccgtttgg acggaaacgt caaagcaaac ggcgaacaaa tcttggaaat
     1741 gcaaaaatcc tatcgcgagt tgaccaaagg acgcgccgat tggctggtgg acgaaaccga
     1801 gaccatactc aatctggcgg cgcaacagct ggtgttgacc ggcaatatcc aaacggcagt
     1861 cggcgtattg gagcatatcg acagccgcct gtcccgtttc gatcaggcag agcttctgcc
     1921 gatcaaacag gcagtcagca gcgacttggc ggaactgaaa aaccgtccct atgtcgatat
     1981 ttccggcacg gcattgcgcc tcgacaggct ggaaaccgcc gtatccggac tgccgctgat
     2041 tttggacggc gtattgaagc cgggcgtaca ggtgaagaac gaagccgctt ctgcttcatg
     2101 gtggcagaac gtatgggaaa aatccctcgg cacattgaag gggctggtcg aaatccgccg
     2161 tttggaaaac aacgatgcca tgctgatttc tcccgaacag gcatattttg tgcgtgaaaa
     2221 cctgcgcctc cgccttttgg atgcgcggac tgcattaatg cagcgcaaca gcgaagtcta
     2281 tcagggcgat ttgaacaatg ccgaagccgc cgtcagacag tatttcgatg ccaagtctcc
     2341 cgccacgcag tcgtggctga aagaactggc ggaattgaag gcgttggatg tgcgcatgac
     2401 ggcggatgac ggtttgaaaa acagcctaaa tgccgtccgc gcctatcgcg acggtacgcg
     2461 catgacggcg gcggaaaatc aagaagcaga acaggcggct tccgaaccgg caaacgaaaa
     2521 cacagcttcc gaaccggctg ccgcatcgga tgtgaagacc atagaagcac cgtccctgcc
     2581 ttcggaacgc aaaccggaac agcctgcaaa aaaacagccc gtaccggaaa aggcagggcg
     2641 ttcgccgtcc gctaaaggag aacgcgcatg aaaacggtag tctggattgt cgtcctgttt
     2701 gccgccgccg tcgggctggc attggcttcg ggcatttaca ccggcgacgt gtatatcgta
     2761 ctcggacaga ccatgctcag aatcaacctg cacgcctttg tgttaggttc gctgattgcc
     2821 gtcgtggtgt ggtatttcct gtttaaattc attatcggcg tattcaatat ccccgaaaag
     2881 atgcagcgtt tcggttcggc gcgtaaaggc cgcaaggccg cgcttgcctt gaacaaggcg
     2941 ggtttggcgt attttgaagg gcgttttgaa aaggcggaac tagaagcctc acgcgtgttg
     3001 gtcaacaaag aggccggaga caaccggact ttggcattga tgttgggcgc acatgccgcc
     3061 gggcagatgg aaaacatcga gctgcgcgac cgttatcttg cggaaatcgc caaactgccg
     3121 gaaaagcagc agctttcccg ttatcttttg ttggcggaat cggcgttgaa ccggcgcgat
     3181 tacgaagcgg cggaagccaa tcttcatgcg gcggcgaaga tgaatgccaa ccttacgcgc
     3241 ctcgtgcgtc tgcaacttcg ttacgctttc gacaggggcg acgcgttgca ggttctggca
     3301 aaaaccgaaa aactttccaa ggcgggcgcg ttgggcaaat cggaaatgga acggtatcaa
     3361 aattgggcat accgccgcca gctggcggat gctgccgatg ccgccgcttt gaaaacctgc
     3421 ctgaagcgga ttcccgacag cctcaaaaac ggggaattga gcgtatcggt tgcggaaaag
     3481 tacgaacgtt tgggactgta tgccgatgcg gtcaaatggg tcaaacagca ttatccgcac
     3541 aaccgccgcc ccgagctttt ggaagccttt gtcgaaagcg tgcgcttttt gggcgaacgc
     3601 gaacagcaga aagccatcga ttttgccgat gcttggctga aagaacagcc cgataacgcg
     3661 cttctgctga tgtatctcgg tcggctcgcc tacggccgca aactttgggg caaggcaaaa
     3721 ggctaccttg aagcgagcat tgcattaaag ccgagtattt ccgcgcgttt ggttctggca
     3781 aaggtttttg acgaaaccgg agaaccgcag aaggcggagg cgcagcgcaa cttggttttg
     3841 gcaagcgttg ccgaggaaaa ccgaccttcc gccgaaaccc attgaaacct tcagacggca
     3901 tcgttgaaat gccgtctgaa aaccatttca cttgacaaac agactgacag aaaacccgta
     3961 tgactctttt aaaaaacgat accttcctcc gcgccctgct caaacagccc gtcgaataca
     4021 cgccgatttg gatgatgcgc caggcggggc gttatctgcc cgaatacaaa gccacacgcg
     4081 cgaaagcggg cagcttcctc gatttgtgca aaaacaccga attggcgacc gaagttacca
     4141 tccaaccttt ggaacgcttc gatttggacg cggcgatttt gttttccgac atcctgaccg
     4201 tccccgacgc aatgggcttg ggactgtatt ttgccgaagg cgaaggcccg aaattcaaac
     4261 gcgccatgca acacgaggcc gacatcgcca agctgcacgt tcccgatatg gaaaaactgc
     4321 aatacgtttt cgacgcggta acttccatcc gtaaagcatt ggacggccgc gtaccgctta
     4381 tcggcttttc cggcagcccg ttcacactcg cctgttatat ggtcgaaggc ggcggcagca
     4441 aagaattccg caccatcaaa accatgatgt actcgcgccc cgatttgctg cacaaaatcc
     4501 tcgataccaa cgcccaagcc gttaccgcct acctcaacgc ccaaatcgac gcgggcgcgc
     4561 aggcggtgca aattttcgac acttggggcg gcgtgttgag cgacgcggcg tttaaagaat
     4621 tcagcctcaa atacatccgc cagatcgtcg ccggactcaa acgcgaaagc gaaggccgcc
     4681 gcgtgcctgt tatcgtattt gccaaaggcg gcgggctgtg gctggaaagt atggcccaaa
     4741 tcggcgcaga cgcattgggc ttggactgga cgtgcaacat cggcgaagca cgccgccgcg
     4801 tcggaaacca agtcgccctg caaggtaact tcgacccgtc cgccctcttc ggtacgccgg
     4861 aatccatccg cgccgaagtc gcacgcatcc tagccgacta cggacacggc agcggccatg
     4921 tcttcaacct cggacacggc atcaaccaac atgccgaccc cgaacacgcc aaaatcttag
     4981 tcgacaccgt acacgagctg tcgcggcagt atcacggcgg gtaagccggc aggaaaccgc
     5041 ccgatatgcc gtctgaagcc gagggatgga aaaataaggc aatgcggcaa tatccgccgt
     5101 gtatggatag tacatgacgg cggcggtgtt gccgcattgg cgcaatccca accgtcccta
     5161 ttttcagacg gcatttttgt tttcagatgc agggaaaacc gatggcaaaa acgcttaaaa
     5221 ccctttacca atgcaccgaa tgcggcggca cttccccgaa atggcagggc aaatgcccgc
     5281 attgcggcga gtggaacaca cttcaggaaa gcttggccgc gcccgagccg aaaaacgccc
     5341 gcttccaatc ttgggcggcg gatacctcga ccgtccaatc cctctcctct gttaccgcga
     5401 ccgaagtgcc gcgcaatccg accggtatgg gcgaactcga ccgcgtattg ggcggcggtt
     5461 tggtcgatgg tgcggtcatc ctgctcggcg gcgatcccgg catcggcaaa tccacgctgc
     5521 tgttgcaaac cattgccaaa atggcgcaaa gccgcaaagt gctgtacgtt tccggcgagg
     5581 aatctgccca acaggtcgcc ctgcgcgcgc aacgtttgga actgcccaca gaaggtgtga
     5641 acctgcttgc cgaaatccgc atggaggcga ttcagacggc cttgaaacag catcagcccg
     5701 aggtcgtcgt catcgactcc atccaaacca tgtattccga ccaaatcact tccgccccag
     5761 gctccgtgtc gcaggtgcgc gagtgtgccg cccaactgac gcgcatggcg aaacagatgg
     5821 gcatcgccat gatactggtc ggacacgtga ccaaagacgg cgcgattgcc ggcccgcgcg
     5881 tgctggaaca catggttgat accgtgctgt atttcgaggg cgaccaacat tccaactacc
     5941 gcatgatacg cgccatcaaa aaccgcttcg gcgcggcaaa cgaactgggc gtgttcgcga
     6001 tgacggaaaa cggtttgaaa ggtgtgtcca acccgtccgc catcttcctc gccagctacc
     6061 gcgacgatac gcccggctcg tgcgttttgg ttacacagga aggcagccgc ccgcttttgg
     6121 tcgaaattca ggcattggtc gatgacgcgc acggcttcac gcccaaacgc ctcaccgtcg
     6181 gactggaaca aaaccgtctt gcgatgctgc ttgccgtgtt aaaccgccac ggcggcatcg
     6241 cctgtttcga tcaggatgtg ttcctcaacg ccgtcggcgg cgtgaaaatc ggcgaaccgg
     6301 cggcggattt ggcggtcatc ctcgcgatgc tttccagctt ccgcaaccgc cctatgcctg
     6361 aaaaaaccgt ggttttcggc gaaatcggct taagcggcga agtccgcccc gtcgcacgcg
     6421 ggcaagagcg gctcaaagaa gcggaaaaac tcggcttcaa acgcgccatc gttcccaaag
     6481 ccaatatgcc gcgcaacgcc aaagagtttc cgaacctgaa aatctacggc gtttcgagtt
     6541 tgcaggaagc catcgatatt tgccgtgaca gcagggaata aacggaaatg ccgtctgaaa
     6601 tcaggtttca gacggcattt ggtttgtggc ggattgaaac aagaaggcat accggcgaca
     6661 gataagattt gcggcaaagt tgcctgtgat atggcaaaac gcacacgccc gtcatccccg
     6721 caagggcggg aatccggaat cgtccgtttc ggcaatgatt gaaaatcacg gtaacccaac
     6781 cgcttggatt cccgacttcg tgggaatgag gggcgtgtgt gtttgatttc catccgccat
     6841 atgtcggcga cgggcttatt cgcctacggt tttttgtatc agtttttcgg cgtttgccaa
     6901 agtgtttgcc acttcgtcaa aaccgatgcg gctgccggcg atgagggcgc gcgtatcggt
     6961 ataggcggcg cgtactttgc cgtctgtttc ggtaacgagg acgcgcaggg gcagttgcag
     7021 ggcgaaggcg gggtctttga ccatcagcgg cgtgccggct ttgggcgtgc cgaagacgat
     7081 gacttttgcc ggctgcatcg ttaaaccgtt tcggtgggcg gcttcctgat ggtcgatgac
     7141 ggcaaaaatg tccatccctt tgctttttat ggcggtttca aggcggctga cggtttcgtc
     7201 aaaactgtat tttgaggtga gggtatgcgt ggtcatagcg gtttcgtttt gggtttgcgg
     7261 ttcgctggca ggatgtgccg aagcggttga aatgcagagt gcggatgcgg caatcagggg
     7321 gagtatgtgt ttcatcgtat ttcctttttc cttttcggtt tgtttatcag gcttcggacg
     7381 gggcggcacg cgccgcgccc gatgttgcgc cgcgtgctgg ggtgtcgtat gccgtctgaa
     7441 agcctgtcct ttcagacggc attgcgtcat ttcatccctt ttttgagcag gtcttcataa
     7501 ccgccgtgat tggcaacatt tgtataacct gcttttttca gctcctgaag ggcggctttg
     7561 gcacgccgtc cgctgcggca gtagaggttg accggcgtgt ctttgtcggg cgcggcttcg
     7621 tgtatgcggc ggacgatttg gtcgacgggg atgttgaccg cgttgtgcaa atgaccttcg
     7681 ctaaattcct gttcggaacg gacatcgatc caaacggccg gatgttgcgc ggtttgggcg
     7741 gcggctaggg gtttttgcgg ggctgcctgc gcggcaaagg cggctgaggc aatgagtgcg
     7801 gcggtaatca ggtgtttgat attcataggg ttctcctgcg gttgtcgtct taagggacgg
     7861 gaagttattt tatctgttcc aaagcggcgg catctatgtc ccaacgccaa acgccgccgc
     7921 ctttgccatc caatccgcgc aaaaacaggt agcggacgga aacggcggcg ggcggttgtc
     7981 cgcgcagttt gaagtagcgt gcggcggcaa cggcgtaaat cagcgcctga aggtaatagt
     8041 ggtggtgcgc gacggcttcg tccatcgcct gttgcgtgta ggcggatgcg tccgcgccga
     8101 gatggtttga tttgtagtcg atgacgcaga tattgccgtc ggggtcttgg cagaccatat
     8161 cgataaagcc gtttaaaaag ccgttgacgg tgtggaagtc gagcgtttcg gcggcggcac
     8221 ggcagacttc gggcaatcgg atatcgtcgc gggcaaacca gtcgcgcagc cgtttgaggc
     8281 cgaagtcttc ggtgtggagg gtaaagccca tttcgggaca gcggcactcg ggcgggatgt
     8341 cggacaggca gtatgccccc gtcagcggcg ttttgcggca ggcttccgcc atttcggcaa
     8401 cggcaggcag ccatatttct tcaaatccgt attttttcag cttgtcggca atgagagttt
     8461 cctgtccggc ggctgcttgt ccgaatttga aatcttcaag aatttcgtgc aggcacagcc
     8521 ccgcctgcgt gcctttcgga aaatcgtgta tcgatatgcc gtctgaagcc gtcggcgttt
     8581 cagacggcat cgccggcacc gaggtttcgg cggcatccaa ggacgggcag gcatcttctt
     8641 cgccgccgtc gggcgtttgg gtatggcggc ttaaggcggt aaagctggtg tggcggacaa
     8701 atcggaatct gcgttcggga atgctgtttg cggcaaattc ggcggtttga ccggcgttgc
     8761 cgcgataggc ggcagggggc ggcgcatctt ctgtgaaggc gaaatccgtg ccggccgggg
     8821 cattgtccgt caaacgccgc cagttgcgtt tgagcatcgc gatgccgtct ttttcccagt
     8881 cataggtttg gcggacggtt tcgcggctgt cttgcggcaa gccttcaatc aggtaggcga
     8941 gggggttgtc ggcggtatcg gaagaatagg cggcgtagat gttgagctgt tcctcggcac
     9001 gcgtcagcgc gacataaagc aggcgcaggc gttccgccat ttcctcatcg gcgtattgct
     9061 tctgttcgtc ttccgacagt tgcgcctttg ccaacagttc ggttcgattt gcgctttggt
     9121 ggaggatttg ccagtcggac ggcccggtat cttgcgcgtc ccacgcaaac gggcagtaca
     9181 ccagcggata ctgcaaacct ttcgaggcgt gcatggtaac gattttgacc aaatcttcgt
     9241 cgctttccag acggatggcg cggttgtcgc cgctgttgtt gccggccagg ctgatttggt
     9301 cgcgcaacca tttgtgcagc gcggcggggt tgcggttttg cgcgtcttcg gcggcaagca
     9361 gttcgagcag ttggaaataa ttggtcaggc tgcgcccgtt gttccggctt aagaggcgcg
     9421 tttcgatgcc gtgtgtttgg gaaaattgct gcatggcggc gaaaatgccg tattgattcc
     9481 agttgtcgag cgcggttcgg gcagattccg cccaatgcaa aatctcgctt tcgttttggt
     9541 tgaagtcgtg caattgctgc gcgtcataac cgaatatgcc gcttgtcagg acaaagcgca
     9601 gcgttccggc gcggcgcggt tcgagccaga agccgatgag tgcggaaagg gcggcggctt
     9661 cgggcgaggc gaacacggat tcgcgcgaaa gcaggacgct ttgcacctgc cgttttttca
     9721 gggcagcgga aaccatcacc gcctcgttgt gcgtgcgtac cagcacggca atatcgcccg
     9781 actgcaacgg gcagcctttg aaattcagac ggcattcggc ggcttcgttg agcgcgtggg
     9841 cgatttcgtc ggcgcaatag tcggcggcac ggcggcgcaa aacgtctttg ttggcttttt
     9901 cattgtcgtt ttcgtgcagc caacgaacct gtacggcagg acgttcgggg gacaacctgc
     9961 tttcggcacg cgccgcaccg acttccgaat agccgatgtt ttccaaaacg aacgggcgtt
    10021 ctttgaggcg gaacagcgcg cctatgctgc cgataagcgc ggcgtggctg cggtagttgg
    10081 tggcgagcgt gtagcggtgc cgcgcgtctt ccgccgcctg aaggtaggcg taaatgtccg
    10141 ctccgcgaaa gctgtaaatc gcctgtttgg gatcgccgac gaggaacagc ggtcggtttt
    10201 gggcgatgaa gattttttgg aagatttcgt attgcagcgg gtcggtgtct tggaactcgt
    10261 cgatcagcgc ggtttcccag ttttcggcaa cggcgcgggc gagggtttcg gcgtgcggat
    10321 tgtcggtcag cgcggtgtgg acatcgagca gcaggtcgtc gaaaccgcgt tcgcggcgcg
    10381 attttttcat ctcggcaagg ctgcggttga ggtatccgat taaatccagt tgcagccgga
    10441 ttattgttgc ttcttccgct tcttcgagtg cgtccaaatc acgcctgaag tctgccagtt
    10501 tctgcaattc ggcaaatact gccgcatcgg gcgttttgcc ttttttcagt ccggcttcga
    10561 gtttgtcgga cgcaagtttc aagagccgtt cgtgcagctt tgcctccaaa aagggcaaat
    10621 ccggcgacag ggctttttgt tccaatgcgt caaacagttc ggcaaaactg tttttccgat
    10681 atgaattgcc gtttaaaagg ggatggatgc gccaaaaagt ttcttttaat tcagccagat
    10741 ttccgcaaat gttttgccag cttgccaaag cggcatcccg ggcttgcggc aaatcggttt
    10801 gcggacggcg gaaattcagg tacgggcggg acaggtaggc gcggatttgg gcaaggacgg
    10861 tttgcggcac agctttgcgt ttaaacgcca atgcggcaag caccggatca ttgctgacgc
    10921 gttcccgcca aaaatcttgc gccgggacaa gcaggcggtc gccgtcttct tcggtcagtt
    10981 cgacatcgaa cggtgcttgg cacaaaaacg cgtagtcgcg caggatgcgc tggcagaagc
    11041 cgtggatggt atagatggcg gcgttgtcga attgcccgat ggcggctttg aggcggacaa
    11101 tcagacgcgt ccggccctct ttttgcaaag cctgttttaa gagtgcgggc aggaaggtgt
    11161 cgcctttgtg gtgttcggcg cagtaggcgg cgatgccgtc tgaaagcgtg tcgtctccaa
    11221 gtttggcaat ttctttactt tctaaaacct gcaacacatc gtccaaacgt gcgcgcaggc
    11281 gtgttttcaa ctcggcggtg gcggctttgg tgaaggtaac gaccaatacg cgctcgacgt
    11341 ttttttgttc caatacgatc aggcgcgtaa acagggcggc aatgccgtag gtttttccgg
    11401 tgccggcaga ggcctcaatc aggttggtgc cggcaatggg gacggtcagc gggtcaaatg
    11461 cttggatggg tgcagacata gtgcgcgctc ggaaaacggt tggacggtat agtggattaa
    11521 caaaaatcag gat
//
LOCUS       DAWEWP010000054        11302 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWEWP010000054 DAWEWP010000000
VERSION     DAWEWP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.054"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            57..337
                     /locus_tag="WGN53_001033"
                     /pseudo
     CDS             57..337
                     /locus_tag="WGN53_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(358..1584)
                     /locus_tag="WGN53_001034"
     CDS             complement(358..1584)
                     /locus_tag="WGN53_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ0140039.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWR
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(1599..2024)
                     /locus_tag="WGN53_001035"
     CDS             complement(1599..2024)
                     /locus_tag="WGN53_001035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140040.1"
                     /translation="MPSEHAAYADLMASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGATVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2129..2788
                     /gene="queC"
                     /locus_tag="WGN53_001036"
     CDS             2129..2788
                     /gene="queC"
                     /locus_tag="WGN53_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ0140041.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2816..3331
                     /locus_tag="WGN53_001037"
     CDS             2816..3331
                     /locus_tag="WGN53_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ0140042.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTEMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDHVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            3338..3760
                     /gene="queD"
                     /locus_tag="WGN53_001038"
     CDS             3338..3760
                     /gene="queD"
                     /locus_tag="WGN53_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ0140043.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4029..4400
                     /locus_tag="WGN53_001039"
     CDS             4029..4400
                     /locus_tag="WGN53_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ0140044.1"
                     /translation="MKLLSTLLVLLVTAEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4397..5035
                     /locus_tag="WGN53_001040"
     CDS             4397..5035
                     /locus_tag="WGN53_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ0140045.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGIPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(5371..5447)
                     /locus_tag="WGN53_001041"
     tRNA            complement(5371..5447)
                     /locus_tag="WGN53_001041"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5411..5413),aa:Pro,seq:cgg)
     gene            complement(5675..6760)
                     /gene="nagZ"
                     /locus_tag="WGN53_001042"
     CDS             complement(5675..6760)
                     /gene="nagZ"
                     /locus_tag="WGN53_001042"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ0140046.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGVGGLKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQEAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(6817..8337)
                     /locus_tag="WGN53_001043"
     CDS             complement(6817..8337)
                     /locus_tag="WGN53_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ0140047.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAIITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDGSRGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVDPSLIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8537..10036)
                     /locus_tag="WGN53_001044"
     CDS             complement(8537..10036)
                     /locus_tag="WGN53_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ0140048.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            complement(10174..10803)
                     /gene="nth"
                     /locus_tag="WGN53_001045"
     CDS             complement(10174..10803)
                     /gene="nth"
                     /locus_tag="WGN53_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ0140049.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(10849..11262)
                     /locus_tag="WGN53_001046"
     CDS             complement(10849..11262)
                     /locus_tag="WGN53_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0140050.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2657 a         3029 c         3147 g         2469 t
ORIGIN      
        1 gttttttcga tacggaaccg gatagaggaa aaatcgaaca ttgcgcctgc cttgctatga
       61 ttcacgaaga aatctgcgcc atgcctatgg gctatgaaac cttgatcggc gatatggcag
      121 cgcactgtca ggcggacaaa aacaacgcat cgtattggcg cgggccttat attgcgaacc
      181 gaaaatccta tttttagatg cagcgaccag ccatttggat attgccaatg aaaaagcagt
      241 caatgcaaac ttgaatggct tgtctatcat aaaaattatg gcggcacaca gaaaggaaac
      301 ggtggaatca gcagatagga aaatgtcttt aggataaaaa tacagtttca aaaatactca
      361 agattgctgc tgttttttcg cctgagcgtc aaactccgcc agcgtcatgt tcaaagtctg
      421 caaacacggt gtcattaccg catcgacagc ttggttcaca tgatcccttt ccacaggcaa
      481 cggacggtaa acgaagagct tgaagagttc gttcaactca atcgaatccg cccccgtttt
      541 caacacccaa ccctgtctgc cggaatagat gtagccgtgc cgcgccagct tttccaaaag
      601 ctcgcccaac tcgtcgtagc ccatattgat atgccgtctg aactcctgaa caggcaaggc
      661 tttgccttct ttttgcgccg catccagaag cagcaggatt ttcaacacgt cgtcgaaccg
      721 tccgcgcgag tcgaagcccc tgcggaacgc ttctccccgc cagtaggaga gtgaagaagt
      781 cagcaccgcg ccgcccaaga ccagcgtcca caacaggttc agccacaaca gaaaaaacgg
      841 cacggcggca aacgcgccgt aaatcgagcg gtagccgtcg aagttgccca tataccaagt
      901 gaagagggaa cgtgcggttt ccagacagaa cgctgttgcc aaagccccga caaacgcctg
      961 ccgcgcggga acgaagcggt ttggcacgaa gcggtacagc ccccacagca aaagcgtcat
     1021 gaaggtcagc gtcgccgccg ttcgcaacgc gcccgaccac tgcggcgcac ctgaggcaag
     1081 cgcggcatcc tgtaccgagc cgaccataaa ggaaatgccc acgcccaaag acagcggccc
     1141 gaacgtcagt aaagcccaat agacgagaaa ctgcatcatc cacggacgct gggaattgac
     1201 ccgccagatg cggttgaacg tattgtctat cgtccgaatc agcatcagcg aggtaacgac
     1261 cagcatcacg ctgccgattg ccgtcagccg gttcgcctgc tcgcggaacg cattgatata
     1321 gtcgaacacc atgtccgcgc cctgcggcac aatggtttgg ttgacgaagg agacgaacga
     1381 atccgaccag cggtcgaaca cggggaaaat cgaagcgacc gccaccatca cggtcagcac
     1441 ggggacgagt gccagcagcg tcgtaaacgt catgcttgcc gccgcctgcg gtacgcgttc
     1501 ttcatcaaag cggcggacga cgaaccatgc aaacgcacag attttattgt ctgccaaacc
     1561 ttgcaaacgt tgtaaaaagg tcataatttc ttgcccggtc agtaagttgg gcattgatgc
     1621 ccgatgttat agccaatttt gccgtcagga acaaatgcct gaactgcggc tgtttcagac
     1681 ggcatcggaa caactgttat gccgtctgaa gaccgaacca ttttaacgga atccgcccat
     1741 gaacccaaat ccccctcaaa atcctcgtcc tctactattt ccaaaacggt agcacccgca
     1801 atcccgcccg ccgaatcgct cgcggcatcg acagcgttga aggttgcgaa gccgtattgc
     1861 gcaccgtccc caaagtctcc gccgtctgcg aagccgtcaa aaaagatatt cccgacagcg
     1921 gctcccgtcc tgaccgccga agaaaacaat atcgccttcg cacaaagcaa acgcttggcg
     1981 gaactcgcca tcaagtcggc ataagccgcg tgttcagacg gcatggcgtt cagatgccgt
     2041 ctgaacgcgt ttgcctgtat aatccgcgtc tttactgtcc aacttcgcgg ttcgcaaacc
     2101 tcccgcgtta ccaaaactag gattcgatat gtcaaaccaa aaagccttgg tcatcttttc
     2161 gggcggtcag gattcgacca cctgcctgat tcaggcaatc caaatctacg ggcgcgaaaa
     2221 cgtccaagcc attactttcc aatacgggca acgccatgcc gtcgagctgg aacgtgcccg
     2281 ctggattgcg caggatttgg gcgtcaaaca aaccgtactc gacttgagcc tgatgcggca
     2341 gattacgcac aatgccctga tggacgacac cgccgccatc gaaactgccg aaaacggcgt
     2401 tccgaatacc tttgtagacg gccgcaacgc gcttttcctg ctctatgccg cgatttacgc
     2461 caaagggcag gggatacggc acatcatcgc gggcgtgtgc gaaaccgact tctccggcta
     2521 tcctgactgc cgcgacgtgt tcgtcaaatc catgaacgtt acccttaatt tggcgatgga
     2581 ctatgatttc caaatccaca cgccgctgat gtatctgacc aaggcgcaaa cgtgggcgtt
     2641 ggcggacgaa atgggcgtgc tggactatat ccgcgagcaa acccacacct gctataacgg
     2701 catcgtcggc ggctgccgcg aatgcccgag ctgtatcttg cgcgaacgcg ggctggcgga
     2761 atatctggaa agtaaaaagg ccgtctgaac acgcgcaaac cataaggaat acgatatgcc
     2821 caagctccat atgttttacc tcggcggcaa tgccggcagg tcgaatatcg aagtgcacga
     2881 catccaattt gccgtgtgcg acgactaccg tgaagccgtc cccgcactca aagccgcgtg
     2941 gttcggcgat gcggacaaaa tccacatcga cggctggcag gttgtcgaat gggcggacgg
     3001 ttacgacatc gccgtatccg aaacgcccaa aacggaaatg ccgtctgaac acgccccgcg
     3061 cctgtatttt gccaatgtcg gcggttatcg cgcggggcag cttgccgagg cgcacgcgtt
     3121 cgggctgttc gccgccgcca cgcctgccga agccaaacaa aaagccctgc aaaccctgtt
     3181 gaccgaccat gttcagcaac ataaagacaa cttaaaagac gtggacaacc tgcttgcgct
     3241 cgaccgcatc ggcaatttcc atatccgcct gaccccgaat ccgcacggca aacccgccga
     3301 aatcggcttt caaggctatt tgcccatttg agaacccatg aaaatcacca aaatcttcac
     3361 cttcgactcc tcgcatatgc tcgacgggca tgacggcaaa tgccaaaact tgcacggaca
     3421 tacctacaaa ctcgaaatca ccgtttcaga cggcattatc aaaggcggcg cgaaagacgg
     3481 tatggtgatg gactttaccg acttgaaagc cattgtcaaa caacacatta ccgacccctt
     3541 cgaccacgcc ttcatctacc acggcggcaa cagccgcgaa tctcaaatcg ccgcactctt
     3601 ggaaggctgg aacatgaaaa ccctgcgcct gccctgccgc accactgccg aaaatatggc
     3661 ggtcgaaatg tacgaccgtc tgaaaaacgc ggggctgaac gtgtgccgcg tcaaattgtg
     3721 ggaaacgccg acatcgtgtg cggagtatga aggggagtag ggaatatctt gaacgtatcg
     3781 atatagtaaa ttccaataag acatgcccaa ccgcgtcatt cccgcgcagg cgggaatcca
     3841 gaccttggtt tttcaggaat atttaaaaat tgcagcaatt ccaaatcttt ggattcccgc
     3901 ctgcgcggaa aggacggttt agagcgtcct tatttgaatt taccgtaaaa cggttttttc
     3961 tcctgtacgg attccccgtt ttttcagacg accctccata tcaaatacac ccattaaaag
     4021 gaatacccat gaaacttctc tccaccctct tagtcctcct tgtcaccgcc gaacatttct
     4081 acatcgcctg gcttgaaatg acgcagattc ccagcgaaaa agcggcggaa atattcaagc
     4141 tgccttatga atttatggaa caaaagcgcg tgcagaccct gttcggcaac caagggctgt
     4201 ataacggctt tctcggcatc gggctggtgt ggtcgcggtt tgccgctccg gataacgcgg
     4261 tgtacggcgc aacggtactg tttctcggct tcgtcctgat tgccgccgcg tggggcgcgt
     4321 tctcttccgg caacaaaggc atactcgtca aacaaggttt gcccgcattt ttggcagcgg
     4381 cggcggtgtt ggcggtatga aaaaaatcaa tgttgccccc gaaaatcccc aataccgcat
     4441 cgtcgaaatc tttgaaagcc tgcaaggcga aggctggaac acgggcatac ccgccgtttt
     4501 cgtccgcttg ggcaaatgca acctcgcctg cggctggtgt gataccgatt atttgacatt
     4561 cggcatgatg agcctgtccg acatcttagg ctgtctgaaa acctatgccg cccgcaacat
     4621 catcatcacc ggcggcgaac cgaccatcca gccgcatctc gatatgctgc tggacatgct
     4681 caaggcggaa ggttatttcc tctgtatcga aaccaacgga ctcaatcccg cgccgccgca
     4741 aatcgactat gtcgccacca gccccaaagc ctgctatgcc gccaaatacg aaaaaagctg
     4801 tatcgaaaca gccgacgaag tgcggattgt tgccgacggc gatgtccttg cgttctgtga
     4861 aaatatggaa cgcaaaatcc gcgcgcatca ttactacctt tcgccctgtg agcaagacgg
     4921 tgcgatgaac atctacgaca ccatccgcca aatcggtatt ttaaacagtc gccccgacgc
     4981 atccgtgcat tggcagttga gcgtgcagac gcacaaatgg gcgggaatag agtagtttaa
     5041 gcagtgtaac tcaaagggac ggcgtacggt tttaccgatg tttgacatac ggggaaagtg
     5101 tgccgcttct gcgtggaaat gccggcattt ccaccgccca atcaggacgg agccttactg
     5161 aataagatgc tgccgttggg tacaagctcg gcttcctaaa ttccgatggt cttttgaacc
     5221 ttgccgatac tctgtgccag tgcgcgcaaa tggcagggtt agggaaaacg aaatgccgtc
     5281 tgaaacagca ttctgtttca gacggcattt ttctgttgac gccaaaaggg aaaaaccgcc
     5341 ccagcaatcc atgccgaggc ggtttgaata tggtcggaat gagaggattc gaacctccga
     5401 ccccttcgtc ccgaacgaag tgcgctaccg ggctgcgcta cattccgaat taagtaaggc
     5461 gtgattatag cgcaaaaaat acggcgtgcc tataccgttt tgcctttttg ccgcgtgtcg
     5521 ggcggattta aaggagtgtg tttgaataca gtgttgataa tcatcattat ctttaagtaa
     5581 ttcaataaga taactttcta cctgaccgaa aaaatcattg cctttccctg acaaacggtt
     5641 gatgaaatcg gcagattgtt gaaacgcagc cggtttaaaa ggcttcgccg acttttacgc
     5701 cgcccgctgt atcctgcggc gaggcaaggc cggcaacaaa ggcttgcgcc tcttggaaat
     5761 ccgccgtctg cataacggct tgcgcggcgg cactgccgag cgtgttggcc atatattgcc
     5821 aacgttgcgc caaagcggga ttgtcaggaa tgcggaaatc ttcgcgcagt tcgtccacaa
     5881 ggtcgggacg gttgcagacg aggacgatgt cgcaacctgc ctcaaaggaa atgcgggcgc
     5941 gttctttgag gccgccgaca ccgcacgcgc cctccatagt caaatcgtcc gagaaaatca
     6001 cgcctttgaa tccgatatcg cggcgcaaaa tttgtttgag ccagatttcg gaaaaccctg
     6061 cgggctttgt gtccacttgt ggataaacga cgtgggcggg cataaccgcc gccatacctt
     6121 cgcggctcat aatgtggaag ggggcgaggt cggcggcttc gagttcggac aggctgcgcc
     6181 tgtcttccgg caagaccaga tggctgtctc cttcgacaaa tccgtgtccg ggaaaatgtt
     6241 tgccgcagga tttcataccg ccttttgcca aacctttttg aagggcgagg gcgaggtggg
     6301 cgaccgcttc gggattgtgg tggaaactgc ggttgccgat gacggggcag tttccccagt
     6361 ccaaatctaa gacgggcgtg aaggacaaat cgatgccgca ggcggaaagc tcggttgcca
     6421 aaacccggcc gacttgtccg gcggcggttt cggcggcgga cgcgccgtct ttgtcccaaa
     6481 tctcgccgag cgtactcatt gcgggcaggc gggtgaagcc ttcgatgaaa cgttgcaccc
     6541 tgccgccttc gtgatcgacg gcgataatga gttcgggtgt gcgcagggct ttgatttcgg
     6601 cggtgagtgt tttgagttgt tcgatgtttt ggaagttgcg gcggaagagg atgatgccgc
     6661 ctacggcggg atcgagcagg cgttgctttt cctcttcggt caggcggaag gcggcaatgt
     6721 ctgccatgac ggggccgcgc ggaatatggg ggacggtcat tgcggtttgc tccaaaaagc
     6781 ttcagacggc atatgccgtc tgaacaggga aaggggtcag gcgttggcgc gttttttatc
     6841 tttcaacaga aaaatcagca ccgccaatac aatgcctgtc gtgccaaagc ccaacagcgc
     6901 ggattttgtc agacccaatg cgaggtagcc cgatgcggcg gcggcggcaa cggttaaggc
     6961 gtaaggcagt tgcgaggtaa cgtggtcgat gtggttgcag cgcgcgcctg tggacgacag
     7021 gatggtcgtg tcggagatgg gcgaacagtg gtcgccgcat accgcccccg ccattactgc
     7081 ggacatacac gggataatca gtgagggatc gactttgacc gccatggcgg cggcaatcgg
     7141 cagcataatg ccgaacgtcc cccagcttgt gcctgtggca aacgccatca cgctggcgag
     7201 caggaagagg atgacgggca ggaagccggg atggatgttg cccgcaacca gtgtggagag
     7261 gtaatcgccg gtgtgcattt cgccgacaac cgtactgatg agccaagcga ggattaaaat
     7321 ggcgattgcg ccgaacatag atttcgcacc ctgccaaacg gctttgggat agtcggcggt
     7381 tttaatcgtg ccgagcgtgc agagaacgac ggcaaggacg ccgcaagtgc cgccgaatac
     7441 cagcgaagtg tttacgtccg tgttttcaaa tgcccccaaa atgctgaagg tttcgcttgc
     7501 ctgcgcgccg gtgtagatca tggcggaaac cgttgaggcg attaaggcca aaacgggaat
     7561 aatcaatgcg taaaccctgc cccggctgcc gtctgaaacg gcagtttcat cgtgggcttc
     7621 gttcaacgcg gcttgttcga aacgcgccat cgagccgatg tcgaaggaaa accatgcgac
     7681 gacgaacacc ataatcaggg caaacagcgc gtaatagttc atcaggctca tggcgacaaa
     7741 cgtccccatc ggcgtgtatt cggtgatttt gtaggtaacg agcaatccgg caagcgtggc
     7801 gataatcgac gcgccccaac ttgaaacagg catcagcacg cacataggcg cggcagtgga
     7861 gtcgaggatg taggcgagtt tggcgcggga aactttaaac ttgtcggtaa cggggcgggc
     7921 aatcgcaccg acggcgaggc tgtggaaata gtcgtcgata aaggttacga acacgaggca
     7981 ggcggtcagc attttcgcgc cgcgccggtt tttaatgtgc cgttttgccc agtcggcaaa
     8041 cgcctgattg ctgccggagt aggtcagcag ggaagtaaaa atacccaaaa gtatcaggaa
     8101 aaccaggatt tttggtttgc ccagcgacca atcgccgtct gaccaagcca agccgacgac
     8161 catgtctttc aggtgtgtca gaccgtcgac ggggttgccg ccgaccaaaa aggcaacgcc
     8221 gaccagaata ccgatgccta aagacagcag tacgcggcgg gtaatgatgg caagtgccag
     8281 tgccaaaaag ggtggcacaa ccgagaaaaa tgaatgtgaa tagtcgatca gctgcatggt
     8341 tatgggggtg ttaagcgtcc ggatggggcg gtttctgtcc gcaacggttc ggattttgtt
     8401 ggcaaaatgg gcgaaagtat tttctgttcc ggaaaatact tatttgaaat caattaactg
     8461 attttttaaa atgcccgtta atcggtattg acgggcattt tatcatttaa aaaatatttt
     8521 ggttaaatta tgtgtgttat tgcaggttta atgcgataaa cagcgtgttg ccacggcgca
     8581 tgatcagcag ggggacgttt ttgcctgcct tgtccatagc tttgcggaaa ccggcttcgt
     8641 cattgacggg gacttgcccg acggcaagaa tttcgtcgcc gcgcctcaag cctgcgcgtt
     8701 ctgccgcgtc ggaaacccgt acgacgacga ggtgtccgcc gctgctgtcg gtatgtgtct
     8761 gaagggtaat gcctgcggat tcgaccgaga acgtaccgga ttgctgttcg gtgtaggggg
     8821 cttcatctgt tttggatgat gcgccgatat gctcggcggc gttgcccagc ttgactttga
     8881 ttgtgatttc ttcgcctttg cgccatacgc cgaggctgac ttcttttccc ggcgtaatgg
     8941 cgccgaccat aacgggaagg tcgccggaag aacgtatttc tccgccgtcg aggctgagga
     9001 cgatgtcgcc cgcctgcagg ccggcacgtt ctgcggggct gccgggcagg attttggcaa
     9061 tcagtgcgcc gccggctttg tccaaaccga acgattgtgc caaaccgtag gatacttctt
     9121 gaataatcac gcccagttgt ccgcgttgga ctttgccggt gtttttcagc tgttcggcga
     9181 cattcatggc aacgtcaatc gggatggcga aggaaatgcc catgaatccg ccgctgcggc
     9241 tgtatatttg cgagttgatg ccgacgacct gtccttttaa gttgaacagc gggccgccgg
     9301 agttgcccgg attgatggca acgtcggttt ggatgaaagg tgtgtagctt tcgttgggca
     9361 ggcttctgcc tttggcggac acgatgccgg cggtcacgct gttgtcgaag ccgaagggcg
     9421 cgccgatggc agcgacccat tcgcctggtt tcaaatcttt gggattgccg attttgacga
     9481 cgggcagctc ttccgttgcg tcgattttca gaagggcgac atcggattgg acatccgaac
     9541 cgatgagttt ggcggtatat tcgcgcttgt cgttgagcag gactttgata ctgcccatgc
     9601 cggtaacgac gtgcgtattg gtcagaatat agccgtcttt gctgatgatg aagcccgaac
     9661 cgaagttcaa tccgccgtca tctgcttctt cttgggggat ttcgggcatg ttcgggacga
     9721 ggcgtttgaa aaattcgtag aacgggtcgc tgtcggcaag cgggtcggaa tcggtttcgg
     9781 cattgctgct gccgttttgg gtgcgcgggg cgggggctgc ctgaatattg acgactgccg
     9841 ggccttcgct ttgaaccagt tggacaaagt cgggcagcag catactgacg ctgccgtcgt
     9901 ctttggtgtg ttcgatgcgt tctacgaaag atgcttcttt tttgtccgca ccgaaaaagc
     9961 tgccggcttt gtcgcagcct gccagcgagg cggcacacag tgctgccaaa gcgaggtatt
    10021 ggtatttttt gaacacgttt tgtcctttgt cggatgccgg taccggcttt aatgccgtct
    10081 gaagcgcatt ttgtcggctt cagacggcat aggttgaaat tctacaacgt ccgtccgaat
    10141 tttcaagcgt ttcattttga agggcggcgg cggtcaggct ttggcgggat attcgcacaa
    10201 atcgttgatg atgcaggttt ggcattgcgg tttgagtgcc ttgcaggtgt agcgtccgtg
    10261 caaaatcagc cagtggtgcg cgtccatcag aaattcttta ggaatgaagc gcatcagttt
    10321 gtcttcgact tcgcgcacat ctttcccggg ggcgattttg gttcggttgg acacgcggaa
    10381 aatatgcgta tcgaccgcca taacggggtg tccgaacgct gtgttcaata ccacgtttgc
    10441 cgttttgcgc cccacgccag gcaacgattc caaagcctcg cggtcttccg gcacttcgcc
    10501 gttgtatttt tccagcagga tgcggcaggt ttgcatgatg tgcttggact tggttttata
    10561 cagcccgatg gttttcgtgt attccatcac gccgtccaaa cccaaatcca gcatcgcctg
    10621 cggcgtatcg gcaaccggaa acagcttcgc cgtcgccttg tttacgccga catcggtcgc
    10681 ctgcgccgaa agcagaacgg caattaaaag ctcgaaaggg gagttgaaat tcagctcggt
    10741 ggtcggatgg gggttggcgg cgcggaagcg ttcgaaaatt tcttggcgga tgtgtctgtt
    10801 catttttttg tgcagtcggt ttgtgcggtc ggcattataa cgcacggttc aggcggcgta
    10861 atattgcatt ccccacagaa tgaaggcgta acgcgccgtt ttgccgataa ccagcatcag
    10921 cccgcttgtc cacggattca accgcagcca gccggcggca agcggcagtg cgtcgccgac
    10981 gacgggcagc caggcaaacg gaagcagcca aataccgaaa cgccgcatca gattcagtgt
    11041 tttttcagac ggcatttttc gggaaggcag caaacgtccc atccaatagg aaaccatact
    11101 gcccaatccg ttggcaaggc cggcgcacag caacgcgccg tatgcgtgtt cgggaaagcg
    11161 gcggacgaac agggcaaagg cggcttcgga tgtgccgggc aggagggtgg cggaagtgaa
    11221 ggcggaaaag gcgagcgcgg cgtaggcgta ggcgggcatc attgcaaaca gtctcaaaca
    11281 ggcaaaaatc ggcgacggat tg
//
LOCUS       DAWEWP010000055        11013 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWEWP010000055 DAWEWP010000000
VERSION     DAWEWP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11013
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.055"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            147..1544
                     /gene="aspA"
                     /locus_tag="WGN53_001047"
     CDS             147..1544
                     /gene="aspA"
                     /locus_tag="WGN53_001047"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ0140051.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFTAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1616..2491)
                     /locus_tag="WGN53_001048"
     CDS             complement(1616..2491)
                     /locus_tag="WGN53_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0140052.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDIGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVEAAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHGGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFSGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2493..3185)
                     /locus_tag="WGN53_001049"
     CDS             complement(2493..3185)
                     /locus_tag="WGN53_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ0140053.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRGHEERGRQEEAFRREQARREQFYREQMRREQA
                     LRQAFERQASRSRHAYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYFYRIIAGFIRF
                     NGGRAVAPEKWI"
     gene            complement(3207..3686)
                     /gene="ybaK"
                     /locus_tag="WGN53_001050"
     CDS             complement(3207..3686)
                     /gene="ybaK"
                     /locus_tag="WGN53_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ0140054.1"
                     /translation="MSKQTYPVTPAVRVLRENGIKFEPFIYPYEEHGGTAQFAHLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3762..4124
                     /locus_tag="WGN53_001051"
     CDS             3762..4124
                     /locus_tag="WGN53_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0140055.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKKDAATE"
     gene            4159..5022
                     /locus_tag="WGN53_001052"
     CDS             4159..5022
                     /locus_tag="WGN53_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ0140056.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPCLAFTAECGSDGICSVVLPHRGILHIAPETALPPYDAAAENAWRLH
                     EILSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVKDSESFTALAVIKFSAAQKELTVPNGGIFKA
                     VHPFFKTENAE"
     gene            5120..6079
                     /gene="ttcA"
                     /locus_tag="WGN53_001053"
     CDS             5120..6079
                     /gene="ttcA"
                     /locus_tag="WGN53_001053"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ0140057.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIIVCLSG
                     GKDSYALLDILRQLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(6173..9112)
                     /locus_tag="WGN53_001054"
     CDS             complement(6173..9112)
                     /locus_tag="WGN53_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ0140058.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSGGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRVEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMTYRELLEKLAQTAKIRMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9125..10816)
                     /locus_tag="WGN53_001055"
     CDS             complement(9125..10816)
                     /locus_tag="WGN53_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0140059.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKKIVINDEL
                     YWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSYGKTIIGK
                     LRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPLDLLKRLV
                     LSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARK
                     AGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDL
                     PLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGAYTAYLCEKRLYLMNAGFTSAD
                     LLAFIRKLDDDAAFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
BASE COUNT         2646 a         2943 c         2604 g         2820 t
ORIGIN      
        1 ataacacccg aaaacaacgt ttcccgtttt cagacggcat acgatatgga caatttcatc
       61 cggccggtat agtgttcgtc ctgcggcggc atctttccga tggcgcattt tttcaatatt
      121 aattatccga cttacaaagg taaaacatga ctgtccgtat cgaacacgat ttattgggcg
      181 accgcgagat tcccgccgaa gtgtattggg gcatccacac tctgcgcgcc attgaaaact
      241 ttaaaatctc cacacaaaaa atttccgacg tgccgcagtt tgtccgcagt atggtgatgg
      301 tgaaaaaagc aaccgcgcag gcaaacggcg aattgggtgc agtaaaaccc gaaatcgccg
      361 ccgccattga aaaggcttgc gacgaagttc tgctgaacaa ccgctgcctc gaccaattcc
      421 cctccgacgt gtatcagggc ggtgcgggaa cttcggtcaa tatgaacacc aacgaagtca
      481 tcgccaacct cgcattggaa gccttgggat acgaaaaagg ccgctacgac atcgtcaatc
      541 cgatggatca tgtcaatgcc agccaatcga ccaacgatgc ctatcccacg ggcttccgcc
      601 ttgccgtgta ttacagcatc ggcgaattgc tcgacaaact gaccgtcctg aaaaacgcct
      661 ttaccgccaa agccgaagaa tttaaagacg ttttgaaaat gggtcgcacc cagcttcaag
      721 atgccgtacc gatgacggca ggacaagaat tccaatcttt ccaagtgttg ttggaagaag
      781 aaatcctcaa cctcgaccgc acgcgccaac tgcttttaga agtcaatttg ggcgcaacgg
      841 caatcggcac gggcgtgaac acgcccaaag gctacgccga actggtggtc aaaaaactct
      901 ccgaagtcag cggcttgcct tgcaaactga ccgaaaacct gattgaggcg acctccgact
      961 gcggcgcata tgtgatggta cacggcgcat tgaagcgcac ggcggtcaaa ctctccaaaa
     1021 tctgcaacga cttgcgcctg ctctcttccg gcccgcgtgc cggcttgaaa gaaatcaacc
     1081 tgcccgaatt acaggccggt tcttccatta tgcctgccaa agtcaatccc gtgattcccg
     1141 aagtggtcaa ccaagtctgc ttcaaagtca tcggcaacga cacgacgatt accttcgccg
     1201 ccgaagccgg gcaactgcaa ttaaacgtga tggagccggt catcgcccaa tgtatgtttg
     1261 aaaccatttc cctcttgggc aatgccgcag tcaacctgtc cgacaaatgc gtcaaaggca
     1321 ttacggtcaa ccgcgaaatc tgcgaacgtt acgttttcaa ctccatcggt ttggtgactt
     1381 atctgaatcc gtatatcgga caccacaacg gcgatttggt cggcaaaatc tgcgcccaaa
     1441 ccggcaaagg cgtgcgcgag gttgtcttgg aacgcgggct gttgagcgaa gaagaactca
     1501 accgcatcct ctctcccgag aacctgatga acccccatct gtaacgttgc agacaaatgc
     1561 cgtctgaagc agggatgctg cttcagacgg cattttgtta tatcaatcag ccggattata
     1621 tgccgaacag tgccttgacc tccgcccaat aaaacaggca ggcgatggaa cacagtgcca
     1681 acacgattcc cgccgtattg attgtgttga ttttttcctt aaaaaacagt gcgcccgaaa
     1741 gcgtccccaa aacaatcacg ccgatattca taccggcaaa aaccagcgtg ggattgtcct
     1801 tcatcatttg gtgcgcggtg atgtaggtta cgatgttcat gaagttcaaa ccgcccaaga
     1861 gtatgccgcc gaccacactc tcaacgcgcc atctgaccga tttggcaaac aggcaggcaa
     1921 acatcagcac acctgccagc acaaatgcaa ccagcaggtt gcctgaaaat gccgtgccgc
     1981 ttttggcaag ctgtttgaac aggatgtcga taatgccgta acccgcccac acgcccagca
     2041 gcaatgccgc ctgccgccac gcgctgcctg attttttgcc gccgccgtgt ttccaaagca
     2101 ggcagaacag cgcggcaaat gcgaggcaca gcccgattag tttgccttcg ctgagttttt
     2161 cgccaaacag cgtcaaggcg gcaacaatcg gcaaaaacag cgacaaacgc tgcgccgcgt
     2221 cggatttgac gataccggcg gcttccacag atttgcccat tatcacgaat acggacggca
     2281 gcagcacgcc caaagcggca aacagcggcc acgtcggcaa aaatgcgccg atattgccga
     2341 tatccggctt caataccagc agggtcagta tgaccgcgac cacataattg acggcgaccg
     2401 cctgcgcgat gtcgattttt ttcttccttg ccattttcag caaaatggaa accgacacgc
     2461 tgaacacgat gctgattaac aggtaagcca tactatatcc atttctcggg tgcaaccgcc
     2521 ctgccgccgt tgaagcggat aaagccggcg atgatgcggt agaaatacca gacgttcgtt
     2581 gcaataataa tcagcacgcc gatacccaaa agggcagtca gcgcacccaa aatataaagc
     2641 caaaatgtgc gccaaaaggt tttaatcagg tattcggtat gtgcggcata gacaatgctg
     2701 tccaaactgt tccgtttcat ataggcgaga atcacgccga ctatgggcat gaacagcatg
     2761 attgcaccca aaccgaacag gatgtaggcg gcgaggacat agttgcgccc gcttccgccg
     2821 ccggacggtt cgtaagcatg gcgcgaacgt gaggcctgcc gttcaaacgc ctgtcttaac
     2881 gcctgttcgc ggcgcatctg ttcgcggtaa aactgctccc tgcgcgcctg ttcacggcgg
     2941 aatgcctctt cctgccgccc gcgttcctca tgccctctga aagacgcgtc gtattgcgtc
     3001 cgtttcgaca ggtcggaaag cgtatcgtaa gcccgttgga tttctttgaa cttttcttcc
     3061 gcctgcggat tacccggatt gcggtcggga tggtatttca tcgccagctt gcggtaggcg
     3121 cgtttgattt cgtccgcccc cgcctgcggc gacacgccca atacggcata caaatctttg
     3181 tccatattgc tccttatact tccccgtcaa accgctgcct gtattgtttt cgggttcaaa
     3241 atatttaaat ctccgggacg gatgccgata atgaacccgc gttttccgcc gttgatatag
     3301 atggtttcca aatccatcac cgactgttcg acgtaaatat ccaattttgt ccggatgccg
     3361 aacggcgttg tgccgccgac cagatagccc gtccacttgt ttgcctgtgc gggcgtggcg
     3421 ggttcgatgt gtttcgcacc caaatgccgc gccagattgc gggttgaaat ctgtttgtcg
     3481 ccgtgcatca agacaatcag ccccttaccg ttttcatctt gcaaaacaat ggttttaata
     3541 accaagtgtt cgtctttgcc gaacaggtgc gcaaattggg ccgtaccgcc gtgttcctcg
     3601 tagggataga tgaaaggttc gaacttgatg ccgttttcac gcaaaacgcg tacggcaggt
     3661 gttaccggat aggtctgttt actcattttt cgtcatgcaa aacatggaaa tcacccaaat
     3721 gcaatgttac catatatccc cctttattga aggaaaccga tatggatatc cgatatttcg
     3781 gcacaacgcc ccgctattcc gaagccgtcg gcgcaaacgg cttgattttc ctctccggca
     3841 tggttcccga aaacggtgaa acggctgccg aacagactgc cgacgtgctt gcccaaatcg
     3901 accgctggct ggcggaatgc ggctcggaca aggcacacgt tttagatgcg gtcatctatc
     3961 tgcgcgatat gggcgactac gcggaaatga acggcgtgtg ggacgcttgg gttgccgccg
     4021 gcagaacgcc tgcccgcgcc tgcgtggaag ctcggctcgc ccgtccggag tggcgcgtcg
     4081 aaatcaaaat aaccgccgtg aagaaagacg cggcaaccga ataaacagat ttcctttata
     4141 tttcagagta cgcaaatgat gaaaacctta ctgccctttt tcggcgtggt gcgcgtcagc
     4201 ggcgaagacc gccaaacctt cctgcacgga cagctttcca acgacatcaa taatcttcag
     4261 accgggcagg cgtgttatgc gacttacaac acgcccaaag gccgcgtcat cgccaatatg
     4321 attgtcgtca accgtggaga cgatttgctg ctgattatgg cgcaagactt gcttgaagca
     4381 acggtcaaac gtttgcggat gtttgtgttg cgtgccaaag ccgtttttga aatccttgaa
     4441 gattacgccg tcggcgcgga attggcagca tccgccgaac cgcttgccgc gcaagaaccc
     4501 tgccttgcct ttaccgccga atgcggttca gacggcatct gttccgtcgt cctgccccat
     4561 cggggcattt tgcacatcgc ccccgaaacc gccctgccgc cttatgatgc cgccgccgaa
     4621 aacgcgtggc ggctgcacga aatcctcagc ggttatccgt ggatatgcgc ggctaccaaa
     4681 gaaactgccg ttgcccaaat gctcaaccag cacatcatcg gcggcgttca cttcaaaaaa
     4741 ggctgctacc cgggacagga aatcattgcc cgtgcccagt atcgggggca ggtcaaacgc
     4801 ggcttggcgg tattgtcggg aaattcggcc gtcgaagcag gaaccctgct gacggcagac
     4861 ggcgaagaga ccggcatcgt gctcgacagc gttaaagatt cggaaagctt taccgccctt
     4921 gccgtgatta aattctccgc cgcacaaaaa gaactgaccg tcccaaacgg cggtatcttc
     4981 aaagcggttc atcccttctt taaaaccgaa aatgcagaat aatccctcta atgccgtctg
     5041 aacctttttc agacggcatt ttaaaatcgc ccctgttttc ggttacaatg ccgcatccct
     5101 aatttttcaa accaccgcca tgtccaaaaa aaccaaacaa gaactcgaaa acaacaaact
     5161 cagcaaacgc ctgcgccacg ccgtcggcga cgcgattaac gatttcaata tgatcgagcc
     5221 ggacgacaaa atcatagtct gcctctccgg cggcaaagac agctatgccc tgttagacat
     5281 cctgcgccag ctccaagcca gcgcgccgat tgattttgaa ctggtcgccg tcaatctcga
     5341 ccaaaagcag ccgggtttcc ccgaagaagt attgccgacc tatctcgaaa gcataggcgt
     5401 gccgtacaaa atcgtcgaag aagacaccta ctccaccgtc aaacgcgtat tagacgaagg
     5461 caaaacgact tgttcgctgt gcagccgcct gcgccgcggc atcctctacc gcaccgcaaa
     5521 agaattgggc tgcaccaaaa tcgccttagg acaccaccgt gacgacatcc ttgccaccat
     5581 gtttttaaat atgttttacg gtggaaaact caaagccatg ccgcccaagc tggtgagcga
     5641 caacggcgaa cacatcgtca tccgtccgct ggcgtatgta aaagaaaaag acttaatcaa
     5701 atacgccgaa ttaaaacaat tccccatcat cccgtgcaac ctctgcggct cgcaacccaa
     5761 cctgcaacgc caagtcatcg gcgatatgct gcgcgattgg gacaaacgct ttcccggccg
     5821 catcgaatcg atgttctccg ccctgcaaaa cgtcgtccct tcccaccttg ccgataccga
     5881 actcttcgac tttgccggat tggaacgcgg tcaaaccctg aaacacggcg gcgatttggc
     5941 gtttgacagc gaaaaaatgc ccgaacgttt ttcagacggc agcgaagaag acgaaagcga
     6001 aatcaaaatc gcgccgcaaa aagccgaacg caaagtcatc aacattctgg cgaacaagcc
     6061 gaaaacctgc ggcgcgtaac cccccgccgt caaaaatgcc gtctgaagcc cgtttggcgt
     6121 ttcagacggc attttaaaat cccgaagcag acggcttcca aacatttgtt tctcagtgtc
     6181 cgttttcaga atgcccgcct gcgggcatat tttgcccgat cagttcggct atcctctcgc
     6241 cgtcaaactg cgttttgaac accaccttga tttctttgga aatctcggca aacagctttt
     6301 cggcatgttt gattttccgc tcttcgcttt ttcgcaaatc ttccgccccg tcagtccctt
     6361 tggcttcaat cacaaagttc agaatctcgc cgcttttggt tttcacgata taggcaaaat
     6421 cgggcgaata cgtgccgccg ccggcaacgg ggattttgat ggagtttctc ggtattttgg
     6481 tgaatacggt tacgccttca atttggttgt tggcgacatt ttcatgttct atatccgaat
     6541 cgtagaaaat ttcgtcgaag agatagccgg cggcaggccg gtgctccgta tcttcaaatc
     6601 tgcccaaatc tgcttttttc accgcgcgcg gtttgccgtc tttatcggta aatttggtcg
     6661 gatggatttt gctgccgaca agccggtaat ccagttcaaa tttatggaag gaatgatgaa
     6721 gcaaaaaccg gttgaagccg tttttgattt gggcgatggt ctgcatattc aaaaaatcgc
     6781 caatgttcag ttcgtcgcgg acgcgataaa acgcctgatg caaagtctgc atgcggattt
     6841 ttgccgtttg tgccagtttt tccagaagct cacggtaagt cattgtgtta aaacggataa
     6901 aatcttcatc ttcaaaactg tctatgcggc gggaaagcat aagcccgttg ttgatataag
     6961 cttcgtttac cgccgtgcgt atgcctgcct gcgggaattt ggcggcgttt tcacgcaaat
     7021 aggcggtaaa taaatcgaca aattcggctt catccttgat tttgtactgc aaaacggctt
     7081 tatggtgaat cagctcccac aaggctttga gttcttcata tttgccttcg cgcatgatga
     7141 tggtgtcttt gccctcgtct ttggcgttgc tgactttgcc tttgtccaaa cctttgggga
     7201 aggcttcggg ataggcggct tttaatttgt catagccgtc ttcggcaaag ttttcattgt
     7261 cgtcaatgat gccgtctgaa aacagttggt ttaccaatac cagcggtttg atatcggggt
     7321 atttttgcaa tattttttgt ttcagctctt cggtaaactt tttggagatt tcttcctgaa
     7381 aagaattgtc gttgatttcg ccgacaagct gcttcacaaa gtctttttcg ctgctatcga
     7441 caaaataatt cagtttgtac ggtacatcgc gcacccgcgc catcagctcg tttaccggca
     7501 ggcgcaggcc gcgtccgact tcttgcagct tggaagtcgt gctgccgctg gaacgcagtt
     7561 tgcaaatctg gaaaacgttg ggattgtccc agccttcgcg cagcgtccat ttggaaaaaa
     7621 taaagcggcg cgggttgtcc aaagacagca gtttttcctt atcgtgcagg atttcattga
     7681 tttcctgctc gattctatcg tcgctgtctg tattgtcttt ggaaaaatag ccgccgtggc
     7741 aggcggatac atcgtccaac gtcttttgca ggtaatcgcg gtaaaacggg tcgctttccg
     7801 ttttcagacg gcgtgccgct tccacgcgaa tccagctttc aaatttatct ttcaggctgc
     7861 ctgaaagctc gttgccgctg cggtagcccg cgatatcgtc aataaaaaac agcgtcagcg
     7921 gcttgatttt gggctgtggc gcgcgttctg ccaaaagcgc gcgttccagc ttgaaatgtt
     7981 cggcaaccgc ccgctgcatc atcgcatcct gcaccgtttg cgaataggaa taagggttga
     8041 tgacggcacc cgttttcaac tccaagccgt tgcttaacac caccacggtt ttattcattt
     8101 tgtcgatttt caaatccgaa atagccggat ggatttgcgc caaatcttcg ccttttgcca
     8161 gtttgaacgt ctgctttttg tccttttcgt ttaattcaaa tttcgcttct ttgccgcccg
     8221 acgacaccag ttttaccgcc gcatccatgc cgccctgcat ttcttcctga aacacgcgca
     8281 cgcctttgac cagcccgtcg ttaaacgcgt ctactgccgt caaacggtaa agcaagttgt
     8341 aatattcatc gttaaatgtc gcaccgtagc gcaaaatata ttgcggtttt aagcgtttga
     8401 tattgcccca cgttttcgcg ctctcccggg tcgggaattt atgcggttcg tccacaatca
     8461 caaacgggcg cacggcggcc aatgcatcaa cgggattgtc aaacaaatct ttcaatgcct
     8521 tgtcgcccgt atcgttcatg gacgacgaat taaccatgcc cgcgttaatc agcagcacat
     8581 ggatttcctt tttgttttcc gctttgacaa attgctcaat cgttatgggc gcattggact
     8641 ttttgccctt attctttttc gcgctttcca ccacataggt tttcaggcgc acgccttcat
     8701 aatcgccgcc gaaatcctgt tcaaaatgct ctgccaaagc cttgctttgc aaaaactgct
     8761 gtgttcccgc cttaatggac aaagtcggca cgaccacgat aaatttgaac acgcccagcc
     8821 aacggtgcag ctcgaacatg gtttgtgtgt aggtataggt tttgcccgtg cccgtttcca
     8881 tggaaatatc aaggatattt tggtcgtccg aacggtcggg gaatcggccg tctataccgt
     8941 tttggctttg cactttcagg atattgtttg cgtattgttt tgcagcaaac aaaagttcgg
     9001 gattttcgtc tgccgtccga tatttgggcg ttgccccgtc aaacacgccc aaaaccgccg
     9061 aaaccgcccg catttggtgc ggctggtttt tctcgtaatt aaaaccgctc atgaattgcc
     9121 tccgtcaaac cctgataacc acattcaact caatctcttt tttattggca taaccgcgaa
     9181 ccgcctggtc aagttcgtgc tgcatggcgc ttgccatatt gctgccgaat acaatcacgc
     9241 ggttgggatt gaaagccgca tcgtcgtcca gcttgcggat aaacgccaac aaatcggcag
     9301 aagtaaaacc ggcattcatc agatacagcc gtttttcgca cagatacgcc gtgtaagccc
     9361 ctaaccgcac aggctcaacc ggcgtggtca gtgccgcccc gtcatacagc gtccaggtgg
     9421 tcagaagcgt ttgcagctgt tcttcgctta attcatcgtt aagcggcaaa tccggttgtt
     9481 cgggcgaaaa atccttgtcc ggatgctgcc tgaaattgtc tgccgtttga aagattttga
     9541 agcccgaatc gcccgtgtaa tcgggatgtt cgacgcggat tttggcggcg gctttttcta
     9601 tgcgggcttt ggtgatgtcg aagatggtcg ggtagcctgc tttacgggct tcggattttt
     9661 cagcggtttt ttcgggaagc tgtacacaga tatagcggcg gttaccgttt tgtccttcgg
     9721 cgttaagctg catcacggcg tgggcggttg tgccgctgcc tgcgaagaag tctaggatta
     9781 ggtcagtttg atttcccttg aaagttgcag aaagaactaa tcttttcaat aaatctaatg
     9841 gttttggtgt ttcaaaagga gaataacctt ttccagtttc aaacaactct tctaattcac
     9901 gaataccatc tttagtaaga tttttactta tatttccaat atttaagatg ctatgaaatt
     9961 tggaaaaatc atcggatact tttctaagtt ttccaataat tgttttccca taagacttcg
    10021 gaattaagat atattcatta ttttcaatat ctttatcaat tttgttttta aactcaattc
    10081 cttttatttc aataatatct tcgtaatgca agttcccata tttgccaaac tgttcgcgaa
    10141 cggcatcttc ctgtatccaa tataattcat cattgattac aatttttttg ccgcgtatat
    10201 cttttggtct ttttagattg tcaaaatgag taatatcttt cgcataaact aaaacagttt
    10261 catgcccatt tacaacatat tttgcattac caccaccttc tgatgtatta acaattaaat
    10321 ctttaataaa attcccttcc ccaaacactt catcacacaa caacttcaac tgcgccgctt
    10381 cgttatcgtc aatcgagata aaaatcacgc cgtcgtcctt taacagttcg cgggcgatat
    10441 acaggcgcgg atacataaag gtcaaccatg cactgtgcga gtttgagcct ttgtcggtga
    10501 aatctaaaat ccgcgcggct tcgtcttcgt caatattggc taagcaggca agttcggctg
    10561 gtgtgaattt gcggtcgtcc tgatagacaa agccgtctga tccggtgttg tagggcgggt
    10621 cgatgtaaat catcttcacg ctgtttgtgt aggcgttttt taagtgtttc aacacttcta
    10681 gattgtcgcc acgaatcagt aggttttggc tgcctgcatt ttcgggtttg gcgttgtgcg
    10741 tcttgtcttc acttatcagg gtttcgggcg gcaggttgcg aagcaggcga gcatatgatt
    10801 tgcccagcca gttcatttcg taaaattcgc gtccgatgtc ggtctgtggc gcaatttcgg
    10861 cttgtaatct gtcgataagg aaatttccgt ctgcgtcaaa acaggcggga aacagttttt
    10921 tgagctgttc gagttgggta gagttggcgg taatgccgtc tgaagtgtag attgcctcgg
    10981 tgttcacccc ggctgtgtcg attagatcag ggg
//
LOCUS       DAWEWP010000056        10568 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWEWP010000056 DAWEWP010000000
VERSION     DAWEWP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.056"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            153..1058
                     /locus_tag="WGN53_001056"
     CDS             153..1058
                     /locus_tag="WGN53_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0140060.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEIETVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
     gene            1104..1253
                     /locus_tag="WGN53_001057"
     CDS             1104..1253
                     /locus_tag="WGN53_001057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140061.1"
                     /translation="MNWQAVKTGKTKHASNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="WGN53_001058"
     CDS             complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="WGN53_001058"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ0140062.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(2634..4427)
                     /gene="lepA"
                     /locus_tag="WGN53_001059"
     CDS             complement(2634..4427)
                     /gene="lepA"
                     /locus_tag="WGN53_001059"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ0140063.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(4570..5271)
                     /locus_tag="WGN53_001060"
     CDS             complement(4570..5271)
                     /locus_tag="WGN53_001060"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ0140064.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFTAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDDLLVGKAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            5457..6569
                     /locus_tag="WGN53_001061"
     CDS             5457..6569
                     /locus_tag="WGN53_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0140065.1"
                     /translation="MTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQDALKNADSAHDLRLAVQLRSRRAQSSGPDLELL"
     gene            6604..7581
                     /locus_tag="WGN53_001062"
     CDS             6604..7581
                     /locus_tag="WGN53_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ0140066.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKGIGKTEFARFAAQA
                     LLCENPSPDHRACGACASCHFFEQGSHPDFYEITPLSDESENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVVLIHPAESMNIQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            7585..7935
                     /locus_tag="WGN53_001063"
     CDS             7585..7935
                     /locus_tag="WGN53_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEZ0140067.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTIGGSRPTFTM"
     gene            7940..8719
                     /locus_tag="WGN53_001064"
     CDS             7940..8719
                     /locus_tag="WGN53_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0140068.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSVAEMVEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDFGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRDQTLEQVAAYTTENFYRLFQKVPDMRAV"
     gene            8745..8960
                     /locus_tag="WGN53_001065"
     CDS             8745..8960
                     /locus_tag="WGN53_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ0140069.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            9061..9372
                     /gene="grxD"
                     /locus_tag="WGN53_001066"
     CDS             9061..9372
                     /gene="grxD"
                     /locus_tag="WGN53_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ0140070.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            9478..10104
                     /gene="upp"
                     /locus_tag="WGN53_001067"
     CDS             9478..10104
                     /gene="upp"
                     /locus_tag="WGN53_001067"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ0140071.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            10127..10447
                     /locus_tag="WGN53_001068"
     CDS             10127..10447
                     /locus_tag="WGN53_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ0140072.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
BASE COUNT         2484 a         2692 c         2822 g         2570 t
ORIGIN      
        1 gggttttatc cggcaggacg gttttttgcc ccaacggaaa atagtcggcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt caggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg tcagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gacggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgc gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtccg agccttgact ccgtccggca tcagattttc
     1081 tgccgtggca aacctgcgta cgattgaact ggcaggcagt caaaaccggc aagaccaaac
     1141 acgccagcaa cggcgcggag cggaaaatgc cgtctgaagc cctttcagac ggcatcgggg
     1201 cgggcagaat caaaaaagcc gcctccgctg gaatggcaat gcaactaaaa taatgtccgt
     1261 atttccatat gccgcccaca ccgtcagtcg gacgaaatgc cctttattca atgggatggg
     1321 tttccacaca aacggaaata tagtggatta aatttaatcc actatattat ccccgaggct
     1381 gccggcaggg aaacaaggcg tttgatgata aaaatgccgt ctgaaacagg gttcggatgg
     1441 catttttgat gttccggcgt aattttccgg cctaacggat tgccgtaccg gccctgccga
     1501 aatcgccgaa gttcatcaaa atgaacattg ccttgccgac aaccagcttg tcatccacaa
     1561 atccccagta gcgcgaatcg gcactgttgt cgcggttgtc gcccatagcg aaatagcgtc
     1621 cttcgggaac tttgcacacg aaaccgctgc cgtcgtcggc atattggcag tgttccaaac
     1681 cgctttgctc tatggaatat ccgttttcag acataatatc ggaggtatat ttgcccaata
     1741 cgggcaggga aacggcaggc tgtccttctt ttttcagaat attgaaggat ttgccgtcca
     1801 aaccgctgtg gaacatatcc gtattgtgga tttcggatgg gtcggtgtcg tcgggataac
     1861 ggtatgtgcc gtcaggaata tcggaagcag gttttccgtt taccgtcaaa actttattcc
     1921 gatattcgac cacatcgccc ggaatgccga caatacgctt gatgtaggtc atctccggtt
     1981 gcaaaggata attaaaaacg accacatcgc cccgttcgat tttgcctgta ggaataaata
     2041 tattgtttaa aacgggtacg cgcaggccgt aggaaaattt gccgaccaaa atgaaatcgc
     2101 ccttaatcag gcccgggcgc atcgagctgg acgggatttg gaacggttcg gcaataaacg
     2161 accggatgag gaacaatacc aaaacggtag ggaagaaact gccgaaataa tcgccgaagt
     2221 ggctgctttc cgagatttcg ggatgagtct tcaggcggta tttgtatacc ccccaagccg
     2281 taccgcacaa tacaacgaaa atcaggaaaa cggcggtaaa gctcataaac agggacaaag
     2341 cggcaaacac gccgaccgcc gtcaggatat aggcgtattc tagaccggaa ctccattcac
     2401 cgttttcctg ccgctttttg tcgcttttga aataaaggat gatgccggca agcagcgcgg
     2461 cagccgcgcc cgacattagc attgtgttca ttgttgttcc ttaatgctta aaaacccgcc
     2521 tgtccgtgca accgttttaa ggcggcaaat tgcaaaattt gtttgcgggc gtgtgcccct
     2581 gaaatcaggg cggtttgagg ggtgttcccg acgcgccgcc ctgtgtgcca gagttatttg
     2641 tcgctcacct gcaaaatcgc caagaacgcg ctttgcggaa tttccacatt gcccacttgt
     2701 ttcatacggc gtttacctgc cttttgtttt tcaagcagtt ttttcttacg cgtaatatcg
     2761 ccgccgtaac atttcgccaa aacgttttta cgcagggctt tgacgttttc gcgggcgata
     2821 atctggctgc cgatggcggc ttggacggcg atgtcgaaca tttggcgcgg aatcagctcg
     2881 cgcatttttg atgccagctc gcggcctcgg tgaaccgcgc tttgacggtg cacaatcagg
     2941 cttaatgcat cgactttttc gccgttgacc ataatatcca gcttaatcaa atcagacggt
     3001 tggaactctt tgaaatggta gtccaacgaa gcataaccgc gcgaagtgga tttgagtttg
     3061 tcgaagaagt ccatgacgac ttcgttcatg ggcaaatcgt aagtcagcat gacttggcgg
     3121 cccatgtact gcatattgac ctgaacgccg cgcttttggt tacacaaagt catgacgttg
     3181 ccgacgtatt cctgcggcac aaggatggtc gcggtaataa tcggctcgag tatggtttcg
     3241 atgctgccga tgtcgggcag tttggacggg ttttcaactt cgattttttc gccgcttttc
     3301 aacacgactt cgtaaaccac cgtcggcgcg gtggtaatca aatccatatc gaactcgcgc
     3361 tccaagcgtt cctgcacgat ttccaagtgc aacagaccca agaagccgca acggaagccg
     3421 aagcccaatg cctgggaaac ttcaggctcg aatttcaacg aagcgtcgtt aagctgcaat
     3481 ttttccaaag cgtcgcgcaa ggcttcgtag tcgtggcttt ccacgggata aagtccggcg
     3541 aatacttggc tttgtacttc ttggaagccg ggcagcggct cagtggcagg gttggcaacc
     3601 agtgtaaccg tatcgccgac tttcgcctga cccaattctt ttacgccggt aatcaaaaag
     3661 cccacttcgc cggctttgag ttcttgtttt tgaaccgatt tcggtgtgaa tacgcccaac
     3721 tgctcaacct gcgtttccgc cttggtgctc ataaagcgca ctttgtcttt cagtttgatg
     3781 gtgccgtttt tcactcgaat cagcatgacc acgccgacgt agttgtcaaa ccacgaatcg
     3841 acaatcacgg cttgcagcgg cgcgttttca tcgccggtcg gcgcaggaat tttggcaacg
     3901 atttcttcca aaacgtcttc cacgccgatg ccgcttttgg cggaacactg caccgcgccg
     3961 accgcatcga tgccgatgat gtcttcaatt tcctgctcca cgcgttcggg gtcggcggct
     4021 ggcaggtcga ttttgttcaa aacaggcaca acctccacgc ccaaatcaat cgcggtgtaa
     4081 cagttcgcta cggtttgcgc ttccacgccc tgcgacgcgt caacgaccaa aagcgcgcct
     4141 tcgcaagccg acagcgaacg ggaaacttcg taagagaagt cgacgtgtcc cggcgtgtca
     4201 atcaaattaa gctgatacac ctgcccgtcg cgcgctttat agttgagcgc ggcggtttgc
     4261 gctttgatgg taatgccgcg ctctttttcg atgtccatgg aatcgagtac ctgcgtactc
     4321 atttcgcgca aatccaaacc gccgcaatat tggatgaagc ggtcggcaag cgtcgatttg
     4381 ccgtggtcga tgtgggcaat gatggagaaa tttcggatat ttttcattag agttgttttg
     4441 aatgtcggac agtgggtttg ggaaatgccg tctgaacaaa cggcgttgcg tccgaatatc
     4501 gggcgcaacg tggaaatagc ccgatattct aacggaaaac cgctgttttg gcatgatttg
     4561 ataaaggttt tataaagatt tgacgatttc tgccaccatt tttgcagaac ttgccgccgc
     4621 cgttttcaaa aattcgtcaa agctgatgtc tgctttttca tctgccgaat cggaaaccgc
     4681 gcggatgatg acgaaaggcg tttccaactg atgacaggtt tgggcgattg ccgccgcttc
     4741 catttctacc gccttaactt cggggaagtg cttgcggatt tccgccacgc cttcgctgct
     4801 gtggacgaag cggtcgccgc tgacaatcag accttgttct accgccgcgc cttcaaacgt
     4861 ccgcgccgcc cgttttgcct tgccgaccaa gaggtcgtct gaaacgaata cggcgggcag
     4921 ttgcggcact tgtccccaaa catagccgaa tgcggttaca tcgacatcgt ggtgtgcggt
     4981 ttccgtgcca atcaccacgt cgccgacttt caaacccttg cccaaaccgc ccgcgctgcc
     5041 ggtgttgatg acgcagtccg ctgcgaattc acggataatc caagccgttg caaccgccgc
     5101 gttgaccttg ccgatgccgc tcaatgcaag caccatgcgt tttcccgcta attcgccttc
     5161 ataggcggta aacttgccga aagagacggc tttgacattt tccatcatct cgcgcaaaag
     5221 ctcgatttct tgttccattg cgccgataac ggctactgtt ttcaaagaca tattgctgac
     5281 ctgttgtgaa tttcggatag aatgcctgat tatacacgct aacacggcag gattgagtgg
     5341 gggcggtttg tccgtgccgt ctgaaacggt ttcagacggc acggcgggtt tttggtagaa
     5401 tgggaaggta cagattgttt gaagattagg ggacgaggat gtttaccgat gaaaatatga
     5461 ccgcaaagga agaactgttc gcatggctgc gccatatgaa ccaaaacaaa ggttccgacc
     5521 tgttcgtgac gactcatttc ccgcccgcaa tgaagctgga cggcaaaatc acccgcatca
     5581 cggacgaacc gctgacggcg gaaaaatgta tggaaatcgc cttttcgatt atgagtgcga
     5641 agcaggcgga agaattttca tcgaccaacg agtgcaactt cgccatcagt ctgccggaca
     5701 ccagccgctt ccgcgtcaat gcgatgatac agcgcggcgc gacagcgttg gtattccgtg
     5761 cgattaccag caagattccc aagtttgaaa gcctgaacct gccgccggtc ttgaaggatg
     5821 tcgcgctgaa aaaacgcggg ctggttattt ttgtcggcgg caccggctcg ggcaaatcga
     5881 cttcgcttgc ctcgcttatc gactaccgca atgaaaattc gttcggacac atcatcacca
     5941 tcgaagaccc gatcgagttt gtccacgaac acaaaaactg catcatcacc cagcgcgagg
     6001 tcggcgtgga tacggaaaac tggatggcgg cgttgaaaaa cacgctgcgt caggcgccgg
     6061 atgtgattct gatcggcgaa atccgcgacc gcgaaacgat ggactacgcc attgcctttg
     6121 ccgaaacggg gcatttgtgt atggcgacgc tgcacgccaa cagcaccaat caggcactcg
     6181 accgcatcat caactttttc cccgaggagc ggcgcgaaca attgctgacg gatttgtcgc
     6241 tcaaccttca ggcgtttatt tcgcaacgcc tcgttccgcg agacggcggc aagggcaggg
     6301 tggcggcagt cgaggtgctg ctcaattcgc ccctgatttc ggagttgatt cacaacggca
     6361 acatccatga aatcaaagaa gtgatgaaaa aatccactac cctgggtatg cagaccttcg
     6421 accaacacct ttaccaattg tatgaaaaag gcgatatttc cttgcaggat gccttgaaaa
     6481 atgccgattc ggcgcatgat ttgcgtttgg ctgttcagtt gagaagccgc cgcgcgcaaa
     6541 gttccggacc ggatttggaa ttgctctgat gaagggtatg gatttccgga cggatggttt
     6601 gaaatgattt atccgtggca taatgagcaa tggcggcaga ttgcggaaca ttgggagcgc
     6661 cgtcccaatg cttggctgtt tgccggcaaa aaaggcatag ggaaaaccga gtttgcccgc
     6721 tttgcggcgc aagcattatt gtgtgaaaac ccaagtccgg atcaccgggc ttgcggggct
     6781 tgtgcttctt gtcatttttt tgaacaaggc agtcatccgg atttttacga aatcactccc
     6841 ttgtcggacg aatccgaaaa cgggcgcaaa ctgttgcaga tcaaaatcga tgccgtcagg
     6901 gaaatcatcg ataatgtgta cctgacttcg gtacggggcg gtttgcgcgt ggttctgatt
     6961 catcctgcgg aaagtatgaa tatccaggcc gccaacagtt tgttgaaagt gttggaagag
     7021 cctccgccac aagtggtctt tttgctggtc agccacgcgg cggacaaggt tttaccgacc
     7081 attaaaagcc gctgccggaa aatggttttg cccgcgcctt cccatgaaga ggcattggca
     7141 tatctgcgtg aaaggggtgt ggcggaacct gaggaacgtc tggctttcca ttccggagcg
     7201 ccgctgtttg atgaggcgga cggtgtccgt gcgttgcgga ttaaactgtt ggatattttg
     7261 gcagaaccga ggttgttgaa gattttggat tacgccgcgc ttttcgataa ggaaaaactt
     7321 ccgctcgccg tatttgtcgg gtggatgcag aaatggctgg tcgatttggg attgtgcctg
     7381 caacacatga aacccgtcta ttatcccgct tatgaagaca ggctgcttca gacggcatcc
     7441 ggtttccgcc cgcgcaatgt atttgcggcg gaggatatgc tcaaacagct tgccccctac
     7501 gggtttcata ctttaaatgt caaaatgcag atcgagcatc tgctcatcaa ttatttggaa
     7561 ttgaagaaag agaacgggtg aattatgtca gacggacaaa atattccggc aaaaatgatg
     7621 tcgttgcagc tgaaagacat gaatctgctg tacagctcct atatgccgtt tttggaacat
     7681 ggcggcctgt ttgtgcagac cgacgacgta ttttccatcg gggacgatat tctgcttgcc
     7741 gtagaaatcc tcaacttccc caaactgttc ctgccaacca aggtcgcttg gattaatcct
     7801 gcgcgtactt cctccaaacc caaaggggtt gggctggcat tcacaaaaca cgaaaactgc
     7861 ctgaaagtca aagaccagat cgaagtcgaa ctgggcagca caatcggcgg cagcagacct
     7921 acgtttacca tgtaacgcca tgcatatcat cgattcgcac tgccacctca attttgaagg
     7981 tttgaaagaa cgcctgcccg aagttttgtc caacatggaa gcaaacggcg tggggcaggc
     8041 gctcgccatc agcgtcagta gggaaagctt ctccgaagtc tttgccatcg ccgaagcgca
     8101 cgaacacatc tattgcacca taggcgtaca tcccgacagt aaggaagccg aagaattttc
     8161 cgtcgcggaa atggtcgaag ccgccgccca tccgaaagtg gtcggcattg gcgagacggg
     8221 tttggattat tactggtgca aaggcgattt gtcctggcag cacaaacgct ttgcagacca
     8281 catcgaagca gccaatcaaa ccggactgcc cgttatcgtc catacgcgtg atgcggcggc
     8341 ggataccttg tctatcctga aagaatgccg ggttaattcg ggcgttatcc actgtttttc
     8401 cgaagacatc ggttttgccc gtgcagcaat ggattttggg ctttatattt ctttctcggg
     8461 aatcgttacc tttaaaaacg cacccttggt tcaggaggcg gcaaaatacg ttccggacga
     8521 ccgcattttg gtggaaaccg atgcgccgtt cctcgccccc gttcccaaac gcggcaggca
     8581 gaacgaaccg gcttttgtgc gccataccgc cgaacatatc gccaaattgc gggaccaaac
     8641 attggaacag gttgcggcat atacgacgga aaacttttac cggctgtttc aaaaagtacc
     8701 cgatatgcgg gccgtctgac cctgtgccga cgataaggaa aaccatgaag gcaattcatc
     8761 cgtatgcatg tccgcgctgc tgccggctgc ctgccaacac gtttcggaca ggcatggcaa
     8821 attccgcttc caaattctgc attgccaaag gcggcagacg ggaagtaaaa aaagacgaaa
     8881 gcggcggcgg atatgccctg tgccatttgc cggacagtag gattgtcgag gagtgggaat
     8941 atttccgttc acaacattga tactgcgcga tatacggcaa atattgtggg aagtttccgc
     9001 ttttgcgtat aatgcgccct acctgacaaa ttttgtcaac tttatcaaaa ggaataagcg
     9061 atggcttcca tccacgacca aattaaagaa gtagtaacga cacaccgcgt cgtattgttt
     9121 atgaaaggta cgaagcagtt tccgcaatgc ggtttctctt cccgcgccgt gcaaatcctg
     9181 aacgcggcgg gctgcaccga ttacgttacc gtcaacgtat tggaaaatcc cgaagtacgc
     9241 caaggcatta aggaatacag cgactggccg accatccccc aactttatgt gaacggcgag
     9301 tttgtcggcg gttcggacat cctgatggaa atgtatgagg caggcgagct gcaagagctg
     9361 ctgaaagcct gatggattcg gcaatgccgt ctgaacgtgt ttcagacggc attttctttt
     9421 ccggcaaatc aaaaaaaagt ataatggcgc gtctcaaaat cacactggaa caccgcgatg
     9481 aacgttaatg ttatcaacca tccgctcgtc cgccacaaat taaccctgat gagggaggcg
     9541 gattgcagca cctacaaatt ccggacgctt gccatcgagc tggcgcgcct gatggcatac
     9601 gaggcaagcc gtgattttga aatcgaaaaa taccttatcg acggatggtg cggtcagatt
     9661 gaaggcgatc gcatcaaggg caaaacattg accgtcgttc ccatcctgcg tgcaggtttg
     9721 ggtatgcttg acggcgtact cgacctgatt ccgactgcca aaatcagcgt agtcggactg
     9781 cagcgcgacg aggaaacgct gaaacccgtt tcctacttcg agaaattcgt ggacagcatg
     9841 gacgaacgtc ctgccctgat tatcgacccg atgctggcga cgggcggttc gatggttgcc
     9901 accatcgacc ttttgaaagc caagggctgc aaaaacatca aggcactggt gctggttgcc
     9961 gcgcccgagg gtgtgaaggc ggtcaacgac gcgcatcccg acgttacgat ttacaccgcc
    10021 gcgctcgaca gccacttgaa cgagaacggc tacatcatcc ccggcttggg cgatgcgggc
    10081 gacaagattt tcggcacgcg ctaactgact gattttcgga gttgatatga attttcaaga
    10141 ctatctcgcc acatttcctt caatcgacca tctgggcggt ttggacgttc aggatgccga
    10201 cggcaaaacg gttcaccaca ttcccgccgt tcagggtaag ctcggttcgc tcaagctgta
    10261 caatgctttg gcagaacgtt ttgacggaaa attggataaa gaagcggcag aacagggttt
    10321 gatatggttt gccgaacacg ttgccgacgc gcgcgcccat cccggcaagc atcccaatat
    10381 cgacctgctt gaaaaagttg tgcaaagcgg cgaaaccttg ctgctcaagc cgcttgccgc
    10441 gcaataattt tcgaccatgc cgtctgaaat ccgtttcaga cggcatttta tcggaaagaa
    10501 gaccgtaaaa cgggcatttt cttttctatt tcaggatacg ggcaatgatg tttcaacaca
    10561 caggacga
//
LOCUS       DAWEWP010000057        10508 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWEWP010000057 DAWEWP010000000
VERSION     DAWEWP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.057"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            185..367
                     /locus_tag="WGN53_001069"
     CDS             185..367
                     /locus_tag="WGN53_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140073.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(429..1430)
                     /locus_tag="WGN53_001070"
     CDS             complement(429..1430)
                     /locus_tag="WGN53_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ0140074.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1507..5340)
                     /locus_tag="WGN53_001071"
     CDS             complement(1507..5340)
                     /locus_tag="WGN53_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ0140075.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKERWDWLLANLDTPLADYKARYGAA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RGRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVQGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5601..5697
                     /gene="ffs"
                     /locus_tag="WGN53_001072"
     ncRNA           5601..5697
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WGN53_001072"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5865..7556
                     /gene="dld"
                     /locus_tag="WGN53_001073"
     CDS             5865..7556
                     /gene="dld"
                     /locus_tag="WGN53_001073"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ0140076.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGY
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7688..7795
                     /locus_tag="WGN53_001074"
                     /pseudo
     CDS             7688..7795
                     /locus_tag="WGN53_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7851..8222
                     /locus_tag="WGN53_001075"
     CDS             7851..8222
                     /locus_tag="WGN53_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ0140077.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8377..8925
                     /locus_tag="WGN53_001076"
     CDS             8377..8925
                     /locus_tag="WGN53_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0140078.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            9060..10151
                     /locus_tag="WGN53_001077"
     CDS             9060..10151
                     /locus_tag="WGN53_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0140079.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
BASE COUNT         2437 a         2884 c         2842 g         2345 t
ORIGIN      
        1 gaaatcccct aaatgtctta gtaggaattt aggggatttt gaggaatttt gcaaaggtct
       61 ctatttgtac tgtcttcggc tcgccgcctt gtccttattt aaatttaatt cactatattt
      121 gcccgtgcgt ttgtgataaa ttcggcactg tatttgatat gattttaaca aatcgggagg
      181 ggcaatgttt tggtatgtaa tcggcttttg ctcctttgtc gtcgcgctgc tgtcgctgtg
      241 ggtcaatgtc ggcgcattcg gtatgcagga agacgatacc ccgcaatcgg attatgaaaa
      301 acgtttgggc ttgggtgcga agctgaaaaa caaaaatacg ccggatacgg aagaaaagca
      361 gcaataaaga tatgaaatgc cgtctgaaag attttcagac ggcatttgct ttgaagcatg
      421 ggagacagtc aaacatttat ctcatgctca aatgcgttca ggattcggcg gacttcatcc
      481 gcatccttaa ccgttcggac gcggtcaaac agcgtttgcg ccgggtcgaa cgtttttttc
      541 atcatgccca accattgctt aagacgggcg atcgggtatt tattgtttgc ctcttttgtc
      601 aggcacagct cgaaaaactg ccgtatccat ttagaaactt cggcaaaatc cgtgtctttg
      661 accggcccgc cgttctcgta ttgcttgatt tgccgcgcca aatcggggcg gatgaccgcg
      721 ccgcgaccga gcatcacgct gttgcagccg ctgattgttt tgatgccgat atagtcttgc
      781 aggctgaaaa cgtcgccgtt ggcggtaacg ggaatattga cgctgtctcg gattttcctt
      841 atccattccc aatgcgccgg cggttcgtaa ccctcggctt tggtacgcgc gtgtacggtc
      901 agtccgcatg cgcccccttc ggcaatcgca caggcgcatt ccaaagcccg gcttttgtct
      961 tcgtaaccga gccgcatttt tgcggtgagc ggaatatgtg cgggcaaacg tccgcgcagc
     1021 gttttgacga tgtggaatat cagttccggc tcttttaaaa ggattgcgcc gcctttgtgt
     1081 ttgttgacgg tgggcgcggg gcagccgaag ttcaaatcga ttttgttcgc accgaagcgg
     1141 acggcttcca gcgcattcgc cgccatattg tccgcatcgc tgcccaaaag ttggacggtg
     1201 cagggcgtgc cggaaaacgt tttgtttccg ttggcgattt cggggacata ttttaaccat
     1261 atggatcggg aatgcacggt atgggtaatg cgtacaaatt cgctgacgca ttcgtcgtag
     1321 ccgccaatac gcgtcagcag gtcgcgcatc acgtcatcga ccagaccctg cataggggca
     1381 aggatgattc gggttttttg tttgggttcg gttgtttgtc cgtcaatcat gattcgggta
     1441 ggtatttgat tcaaacaatg ccgtctgaag gctttcggcg ttcagacggc atttccgtgt
     1501 ccgttgttac agcaatactt tttccacgcc gccgttgttg gcttttttca caaactcgtc
     1561 tagccagttt tcgccgagga tgtgtttcgc catttcgatg acgatgtagt cggcaggcat
     1621 attgttgtcg tcggtgtagc ggctcaagcc ttgcaggcag gctgggcagg aagtaagcat
     1681 tttgacgggt tcaccctgcg gcagctcttt gaggtttttc tcgatttcct cttgtttgcg
     1741 gaatttgact tgggtggcga tatcgggtcg tttgacggcg aacataccgg actcgccgca
     1801 gcagcggtcg cttaagacga ctttctgccc catcaggctg ctggccattt gggtggcgtt
     1861 catggttttg atgggggtgt ggcaggggtc gtggtagagg tattgctgac cttgcacgcc
     1921 gtcgagtttc acgccttttt cgagcaggta ttcgtggatg tcgatgatgc ggcagccggg
     1981 gaagatttct tcaaagcggt atttttcgag ctggtcgtaa caagtgccgc aactgacaac
     2041 gacggtctta atgtcgaggt agttgagggt gtttgccata cggtggaaag ccacgcggtt
     2101 gttggtgctc atttcttcgg ctttagcctt gttgccgccc gcgtcttgcg gatagccgca
     2161 acacatatag ccgggcggca ggacggtttg tacgccgacg tgccagagca tggcttgaac
     2221 ggcaagtccg atttggctga acaggcgttc cgaaccgcaa cccgggaagt agaacacggc
     2281 ttcggcatct tcgggcgcgg cggggttgcg gatgatgggg atgcttttgc cgtcttcgat
     2341 gcccaataag gagcgcggtg ttttggcggg tacgcttttc ggcaaaggac ggttgatgaa
     2401 atggataacc tgttctttaa ttggggcttt gccgacggtt gccttgggtt cggctttttg
     2461 ctttttcgtg ccgatcggaa gaagcttgcc gattttgtag gcaaagtttt gcgctgggaa
     2521 gccggtctgt atcatggcgg cgcgaagggc tttgatggtt ttcgggccgg tggcgttcaa
     2581 aaacgccata cccatagctg cggcaggcgc aaatcgtttg tggccggaat cggcaaggta
     2641 gttgcggacg gctacggtta cgtcgccgaa gtcgatgttg acggggcagg gtttgacgca
     2701 gcggtggcac acggtgcagt ggtcgccgat gtccatgagt tcttcaaagt gtttgacgga
     2761 aacgccgcgg cgggtttgtt cttcgtataa gaaggcttcg gtcagcaagc ccacgccgag
     2821 gattttgttg cgcggactgt acagcaggtt ggcacgcgga acgtgagtag agcagacggg
     2881 tttgcatttg ccgcagcgca ggcagtcttt gacggaatcg gcaatcgtgc cgaggtctga
     2941 tttttccata atcagcgatt ccgcgcccaa aagctcgaaa gacggcgtgt aggcgttgcg
     3001 taagtccgag cctttcatca gtttgtggcg gttgaaggtg tgtttgggat cgacttggtt
     3061 tttgtagtcc caaaacggct gcatttcttc atcgcttaaa aattcgagct tggtgatgcc
     3121 gatgccgtgt tcgccggaaa ttacgccgcc gagcgaacgg gcgattttca taatgcgttc
     3181 gaccgaacgg taggctgtct gaagcatttc ggcatcgtct gagttgaccg gaatattggt
     3241 gtggacattg ccgtcgccgg cgtgcatatg cagggcgaca aagactctgc cgcgtacggt
     3301 tttggcgtgg attttgccca agccttggat aatcttggta tcggttttgc cgctgaagat
     3361 ttcggcgagt ggtttcatta cgtctgcttt gacagacacg cgcaggcgga aatcgcggaa
     3421 tgcggtaaag cagctttcat tgtctttggc ttcgggcgcg gcgtggacgg ctgcaccgta
     3481 gcgggctttg tagtcggcaa gcggcgtgtc caaattggcg agcaaccagt cccaacgttc
     3541 tttgacggcg gcaacgtggg ccaaggcgtg tttgccgcgt tcgcccaaaa gttcggcggt
     3601 cggcaggtca gtgcccattt tgtcgatggg gagtttgccc gaaagatatt gctccaaggc
     3661 ggcacagagt ttgagcttgt tttggatgga aagctcaatg ttgatgcgtt cgatgccgtc
     3721 cgaatactcg ccgagccttt cgagcgggat gaccacgtct tcgttaattt taaaggcgtt
     3781 ggtgtgcttg gcgatggcgg cggtgcggct gcggtcgagc caaaaggttt tgcgcgcttc
     3841 gggagacacg gcgataaagc cttcgccatc gcgggcgcgg gcaagttcgc aaatgtgttc
     3901 ggcggctgcc tctacggcgg cttcgtcgtc ggaaaccacg tccgccagca agaccatttt
     3961 cggccgtccc ttgcccgccg ctttggttgc gtagccgacg gcgcggacgt aacgccagtc
     4021 caaatgttcc aaacctgcca gccgaacgct gtcgtgggcg agcaggaaat cgcggatttc
     4081 gacgatagaa ggcgtagcgg tggcgaccgt gccgaaaaac tccatacaca cagtgcgcgt
     4141 gtatttcggc attttgtgca acacgaaagc aacgccggtg atgatgccgt ccgtgccttc
     4201 tttctgtacg ccgggcaggc cgctcaagaa tttgtcggta acgtctttgc ccaaaccgac
     4261 tttgcgaaat ttgtggccgg gaatttccaa acgttcggtt ttaacgatat tgaggccgtc
     4321 tgaatccagc gtatgtacgt caaacacggc ggtttcttca tcgtggattt tgccgaaatt
     4381 gtggcgcacg cgctcgatac gcagccattc gccttgaggg ttgaccatct gccagtaggc
     4441 gaggttgtcc aacgccgtcc cccacaacac ggcttttttg ccgcccgcgt tcatcgccac
     4501 attgccgccc acgcaggacg catcggcaga agtcggatcg acggcgaaca ccaaacctgc
     4561 ctgatgcgcg gtttcttcca cccgccgcgt aaccacgccc gcgccgcacc ggataatcgg
     4621 atgcttgccg tccaatcctg ccagctcaac gtattccacg ccgcgatgct tgtcgagctt
     4681 ttcggtattg atgaccgcgc tcattgcgtc caaaggaatc gcgcctccgg tataacccgt
     4741 gccgccgccg cgcgggataa taaccaagtc caattcgatt aaagcgcgca ccaaaggcgc
     4801 gacttccgcc tccgtgtcgg gattgacgac gacaaacgga tactccacgc gccagtcggt
     4861 tgcatcggta acgtgcgtta cccgcgccag cccgtcgaac ataatattgt gcggcttggt
     4921 gattttgctc aaacgctcca aaatctgccg ccgtttttgg ctggtttcat caaaactgct
     4981 atcaaaacgc tcgactgctt tttctgctgc ggcaaccaac acatccactt gccgattatc
     5041 gtcgcggcgt ttgcggattt catttaagcg gtggcgcatt tcacgtacca gcgcggcgcg
     5101 gcgttttggg tgctccagca agtcatcgac cagatacgga ttgcgcacga ccacccaaat
     5161 atcgcccaac acttcaaaca gcatccgcgc cgaacgcccg gttttgcgct gtccgcgcaa
     5221 gtcttgcagg atttgccacg cctcatcgcc cagtaggcgg atgacgattt cgcggtcggt
     5281 gtaggaagta tagttgtagg gaatttcccg aatacgctga ggggcggtag tcgtggtcat
     5341 ggtgtgtccg tgtttggcag tttttatttg aatgccgtct gaaaattcgg tttcaggcgg
     5401 gtttgaaaag gcgataatgt agttcaattt ttgatatttt tcaatgccga aacaggaaat
     5461 acccttatag aaacaaaata acgctttgag aaattgttga caatattatc aaatataaat
     5521 tttaggtggt gtttccggcg tatgaccgta tcaaaacggt tgagtttttc agaaaacccg
     5581 ttataatgcc gtttctctga ggcgggtctc cccgcatggc aaatcggaac accgggtcag
     5641 gggcggaagc cagcagccca ctccgatgcg ccagtgccgg gggtttggcc cgccgcccta
     5701 tttgaaacgc cggagctttg atgttccggc gttcccgtat cgcaccgttg ccgtctgaaa
     5761 gccgttcaga cggcattttc tttactttgc cataacgtat aactacaatg tgaaaacaat
     5821 tccaatgtgc atatgcacct gccgacaatt ttaaggaaac tgccatgagc gcatcacaat
     5881 tactgtcaag actgacccaa accgtaggcg aaaaatacat catcaccgac cccgcgaaaa
     5941 ccgaacaata ccgccaaggc taccgcttcg gcgagggtaa ggcgttggcg gtggttcgcc
     6001 ccggaagcat tctggaaatg tggaaaattc tgcaggcgtg cgtcgaagca gacgtgattg
     6061 tgattacgca ggcggcgaat accggactga ccggcggttc gacccccgac ggcaacgatt
     6121 acgaccgcga catcgtgatt gtgaacacca tgcggatgaa catcatccaa accatcaaca
     6181 acaacgaaca agtcgtctgt ctgcccggtt cgaccctgaa ccagcttgaa ttgctgctga
     6241 aacctttggg gcgcgaaccg cattcggtca tcggctcatc ctgtatcggc gcgtccgtct
     6301 tgggcggcgt gtgcaacaac tccggcggcg cgttggtaca gcgcggcccg gcctacaccg
     6361 aaatggcgtt gttcgcccaa atcaacgaag aaggcaggtt ggaactggtc aaccacttgg
     6421 gcatagactt gggcgacacg cccgaagaaa tcctgaccaa cctgcaaggt catcattatc
     6481 aaaacaaaga catcaaacaa gacgcgggca aaggatacga ccacgcctat tgcgaacacg
     6541 tccgccaagt ggacgaaccg accgccgcgc gtttcaatgc cgaccccgcc cgccattacg
     6601 aagcctcggg ctgcgcgggc aaactgatgg tttttgccgt ccgtttggac accttcccgc
     6661 aagaaaaaca aaccgccgtg ttctacatcg gcacgaacga catcaacgag ctgaccgaca
     6721 tccgccgcgc cgccttgggc gaatttgaaa gcctgcctgt ttccggcgaa tacatccacc
     6781 gccacgcttt cgatattgcc gacgtgtacg gcaaagacac gttttacgtc atcaaaaaat
     6841 tcggtacgca ccaactgccg aaattatttg acttgaaggc gcgcgtggac aggttcggca
     6901 aaaaagccag cttcctgccc aaacattttt ccgacaaggc catgcagttc gtcagcaaat
     6961 acctgcccga ccacctgccc aaatccatgc gcgattaccg cgacaaatac gaacaccacc
     7021 tgattctgaa aatgggcggg aaaggcgtgg atgaggcgcg cgcgttctta aaagaatatt
     7081 ttgcacacca cagcggcgcg tttttcgagt gcaacgccga agaaacccaa gccgcgatgc
     7141 tgcaccgttt cgccgtcgcc tccgccgcca tccgctaccg cgccgtgcat gatgacgaag
     7201 tggaagactt ggtcgcgctc gacatcgccc tgcgccgcga cgatcgcgac tggtttgaaa
     7261 aactgccgtc ggaaatcgac aataaaatca tccataaact ctactatgga catttcatgt
     7321 gccacgtttt ccatcaggat tacatcatca aaaaaggcaa cgactgcatg gcgttggaac
     7381 acgaaatgct gcatctttta gaccaacgcg gcgcgcaata tcccgccgaa cacaacgtcg
     7441 gtcatttgta tgaagccaaa cccgcgctca aacagtttta ccgcaaactt gacccgacca
     7501 acagcttcaa cccgggcgta ggcaaaacca gcaaaaagaa aaactgggcg gaataagcgc
     7561 gtccgctttg aaggcaggca atgccgtctg aaggcaaaac gccgttcaga cggcattgtc
     7621 gggtttgaag ttccccatcg ggtaatcgct ttcaagccgt atgccggcac aggcgcgcgc
     7681 gcctcccaaa tatagtggat taaatttaaa tcaggacaag gcaacgaagc cgcagacagt
     7741 acagatagta cggcaaggcg aggtaacgcc gtactggttt aaatttaatc cactataaag
     7801 tctgatccta ccttcccaaa gggcagggtt tcaaccgaaa aggaaacacg atgaaaccat
     7861 acaaaatcta cacccatccc gccctgccgc cccaagccgt caaacaaggc tggtcgtggc
     7921 cgggcctttt gttcggcacg ctgtgggcgt gtttcaaaag aatgtggggt ttggggctgg
     7981 ggctgacggg cgcgatattc gtgctggcgg tgtttgccca actggtttac ggcgacacgc
     8041 ccgccacaga ctcggcgttt aacgtattgg gcttggcggt ttccgtctgg ttcggcgcaa
     8101 agggcaacag cctttatgca cgccaccttt tatcgcgcgg ctataccgaa ttgcccgaaa
     8161 cggttgaggc agccaatccc caagccgcat tggcgcaata tttcgggcgc gggggccgat
     8221 aagcgcgttt tcttttccat catgccgtct gaaaaatgtt cagacggcat ttcttataac
     8281 cttttgtttg cagcacataa ccaatcagtc tttcctattc cgttccgaaa tgcataccat
     8341 gcgcccgtac ccaatatttg cgaacaagga aagaaaatgg cacgtttaac cgtacacacc
     8401 ctcgaaaccg cccccgaagc cgccaaagcg cgcgtcgagg cggtacttca aaacaacggc
     8461 tttatcccca accttatcgg cgtattatca aacgcccccg aagccttggc gttttaccaa
     8521 gaagtcggca agctcaacgc cgccaacagc ctgaccgctg gcgaagtcga agtcatccag
     8581 atcatcgccg cccgtaccaa ccaatgcggt ttttgcgtgg cagggcacac caaactcgca
     8641 accctgaaaa aactcctgtc cgaacaatcc gtcaaagccg cgcgcgcttt ggcagcaggc
     8701 gaatttgacg atgccaaact cggcgcgctc gccgccttca cccaagccgt aatggcgaaa
     8761 aaaggcgcgg tatccgatga cgaactcaaa gcatttttcg acgcgggcta caaccagcag
     8821 caggcagttg aagtcgtgat gggcgtagcc ttggcaactt tgtgcaacta cgtcaacaac
     8881 ctcggacaaa ccgaaatcaa ccccgaattg caggcttacg cctgatacgg caaaccgccc
     8941 gaatatcggg cggtttttca aaaacacccc ctcaaacaaa aacaagccgc ctaagcggca
     9001 gggaagcagc cgcgcatcag gcgcgccccc gatttccccg caaaccttaa ggaacaaaga
     9061 tgaacgccca aaccctgatt gccaacgttg ccgaattcgt caaaaccaag ctcaaaccca
     9121 tagtggacga catcgaccgc aaaggatact accccgaagc atttatgcgc gaactcggcg
     9181 caatcggtgg attcggcgca gtcggtacag aagccgaagg cggcaacggc ttgggtttgg
     9241 caacgcaaat cgccgtattg cgcgaaatcg gcaaagaatg cggcgcaacc tctttcagtg
     9301 cgtggtgtca ggcggcttgc gcgtggtatc tgcaccaaac gcccaaccgg gccgtcaaag
     9361 acaaatacct tgccgacatc ctgcaaggca aagtattggc gggtacgggc atgtccaata
     9421 ccgtcaaaca ccttgccgac atcgaaaaac acaacctcca agccgagcgc gtggagggcg
     9481 gctacacagt caacggcgcg ctgccgtggg tgtccaacat cggcgaagac cacatttggg
     9541 cgaataccgc ccaaatcggc ggcggctacg tcatgttcat cacaggcgga caatgggaag
     9601 gcgtaagcct gcaaaactgc cccgaatttt gcgccctcga aggcacgcgc accttcagcc
     9661 tgaacttcaa agacgtattt atccccgacg aagacatcat cgccgcgccc gaacagtttg
     9721 ccgactacat ccaaagcatc aaagcaggct ttatcctcct gcaaatcggc atcggcgcgg
     9781 gcgtgataga cggcagcctc ggcatcatcc gcctcgccaa cgtcgtcaac gccgaagtca
     9841 accgctatct cgacgacggc tacgacagcc tcaaaacaag gctggacggc gcgtgggcag
     9901 aaaccgaacg gctcgccgga ttggcttgga gcggcacgcc cgacaacctt accaccctca
     9961 agctgcgcga agccgccgcc gtactgacac ttgccgccgc ccaatccgcc gccctgcatt
    10021 ccggcgcaaa aggctacctg atgcggagtc ccgcccaaag gcgcgtccgc gaagcaatgt
    10081 ttgtcgccat cgtaactccc gcgatcaaac acctccgcaa agaaatagcg gcaatcgaag
    10141 ccgcaaaata aacaggcgga aaaaaccgaa acccccatcg cgtcattccc gcgaaagcgg
    10201 gaatccagcc cccaacgcaa caggaatcca tcggaaaaaa cagaaacccc caaaccgtca
    10261 ttcccgcaaa agcgggaatc cagcccccta acgcaacagg aatccatcga aaaaaccgaa
    10321 acctccaaac cgtcattccc acggaagtgg gaatccagta accgaaaaac cacaggaatc
    10381 tatcgaaaaa aaacccaacc cccaaaaaaa ccgggcggat acctcaccat cctcccaaac
    10441 cctgatttat agcggatgaa caaaaatcag ggcaagacgg cgaaaccgca gacagtacaa
    10501 atagtacg
//
LOCUS       DAWEWP010000058        10396 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWEWP010000058 DAWEWP010000000
VERSION     DAWEWP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.058"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            30..809
                     /gene="xth"
                     /locus_tag="WGN53_001078"
     CDS             30..809
                     /gene="xth"
                     /locus_tag="WGN53_001078"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0140080.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            796..1140
                     /locus_tag="WGN53_001079"
     CDS             796..1140
                     /locus_tag="WGN53_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140081.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            1151..1681
                     /locus_tag="WGN53_001080"
     CDS             1151..1681
                     /locus_tag="WGN53_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0140082.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(1774..2889)
                     /gene="asd"
                     /locus_tag="WGN53_001081"
     CDS             complement(1774..2889)
                     /gene="asd"
                     /locus_tag="WGN53_001081"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ0140083.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGAETNKILGRSDNPTVIDGLCVRIGSMRCHS
                     QALTLKLKKDLPISEIEAILAGANDWVKVIPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            3065..3646
                     /locus_tag="WGN53_001082"
     CDS             3065..3646
                     /locus_tag="WGN53_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0140084.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(3708..4562)
                     /gene="folD"
                     /locus_tag="WGN53_001083"
     CDS             complement(3708..4562)
                     /gene="folD"
                     /locus_tag="WGN53_001083"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ0140085.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQNNLHTPCLAVVLVGGD
                     PASAVYVRNKKTSCQKCGIKSLSYELPESTSQEELLALVDRLNVDSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            4785..4862
                     /locus_tag="WGN53_001084"
     tRNA            4785..4862
                     /locus_tag="WGN53_001084"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4820..4822,aa:Pro,seq:tgg)
     gene            4887..4963
                     /locus_tag="WGN53_001085"
     tRNA            4887..4963
                     /locus_tag="WGN53_001085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4921..4923,aa:Arg,seq:tct)
     gene            4991..5066
                     /locus_tag="WGN53_001086"
     tRNA            4991..5066
                     /locus_tag="WGN53_001086"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5024..5026,aa:His,seq:gtg)
     gene            5202..5708
                     /locus_tag="WGN53_001087"
     CDS             5202..5708
                     /locus_tag="WGN53_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ0140086.1"
                     /translation="MVDAWSVPDFESKICPPEALSERLARLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKDGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            5705..7483
                     /locus_tag="WGN53_001088"
     CDS             5705..7483
                     /locus_tag="WGN53_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ0140087.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKVDGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            7596..8420
                     /locus_tag="WGN53_001089"
     CDS             7596..8420
                     /locus_tag="WGN53_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ0140088.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(9183..9971)
                     /locus_tag="WGN53_001090"
     CDS             complement(9183..9971)
                     /locus_tag="WGN53_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ0140089.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(10185..10379)
                     /gene="thiS"
                     /locus_tag="WGN53_001091"
     CDS             complement(10185..10379)
                     /gene="thiS"
                     /locus_tag="WGN53_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ0140090.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQQPFAVAVNTVFIPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         2216 a         2668 c         3109 g         2403 t
ORIGIN      
        1 catttttatt gtgagaactt atttttatta tgcttaaaat catttccgcc aacgtcaacg
       61 gcatccgctc cgcctacaaa aaaggttttt acgaatatat cgccgcatcg ggcgcggaca
      121 ttgtctgcgt gcaggaactc aaagcgcagg aggctgattt gtctgccgat atgaaaaatc
      181 cgcacgggat gcacggccat tggcattgcg ccgagaagcg cggttacagc ggcgtggcgg
      241 tgtacagcaa acgcaaaccc gacaatgtgc aaatcggtat gggcattgaa gaattcgaca
      301 gggaagggcg gtttgtgcgt tgcgatttcg acaggttgag cgttatttcg ctttacttgc
      361 ccagcggcag cagcgcggaa gaacgccagc aggtgaaata ccgtttcctt gatgcgtttt
      421 accctatgct cgaagcgatg aaaaacgaag gacgcgacat tgtcgtttgc ggcgactgga
      481 acatcgccca ccaaaacatc gacctgaaaa actggaaagg caatcagaaa aattcgggct
      541 tcctaccgga agaacgcgag tggataggca aggttatcca caagctcggt tggacggata
      601 tgtggcgcac gctttatccc gatgtgccgg gctacacgtg gtggagcaac cgcgggcagg
      661 cgtatgcgaa agatgtcggg tggcgcatcg attatcagat ggttacgccc gaacttgccg
      721 ccaaagccgt gtccgcacac gtttataaag atgaaaaatt ttccgaccat gcgccgctgg
      781 tcatggagta tgactatgct gccgaataaa gttttgggta aatatgattg gaatgtggac
      841 ggaaaaacag gaataggcgc agcatgggtt gtcgctgcgt ttatcctgcc catattggtg
      901 tgggcgatat ttatgttgtc gcggatgcaa ggctggcttg cgccgaccaa ggcaaacccg
      961 acatgggcgt tggtgtggct gctgatctgc atgccctgcc tgctgattgc gtccaaatgt
     1021 ttgggttgga aaggctggcg gcgggtggtg aatatttttg cctgcctgac cgtctgcgcc
     1081 atattgagcg tacccgcttc gctgctcatc gcctttaccc tccgggacct gctgaaatag
     1141 gcggggcggt atgcagggtt ttgaactcga agatttgacg ctttggctga tacgcgatgc
     1201 cggagaggat cagatgtgga tagaccgttg ggcgatcagt tatcccgtcg tgcagatgtc
     1261 cgaagcgtcg gccggtcaaa gcatagggga atggcaggca gggcttcgga cggcatttga
     1321 acgcatacgt ggcaaatacg tcgccgttgt cgcacacggt gcgggcgcgg ccgcattttt
     1381 ggcgtggctg tatcaggcag acatcctgac acggaagaaa attgccaaca tcatccttgt
     1441 accgcagcgt cccgatattt ttcccgacga tgcggaacac actttccaac gcgtccgctg
     1501 tccctgccgt gccgcattgg ttgtacccga acacggcggc gtgccgcacg gttgggcgca
     1561 aaaacaggcg gatttgtgga acgcccgcct gttggtttcc ccgcattcgg gcagtttgaa
     1621 cggcatgctc ggcggctggc agtggggtat gaagctgatg caggaaatgt tgctggcgtg
     1681 aacgcttgcc ttatcggaat ttcggttaac tggggttgca aggaaaatgc cgtctgaaac
     1741 aggcttcaga cggcatttcc attcaaacag ggcttacagg ctgcccaaca cgatacgcaa
     1801 tacgcggcgc agcggctcgg ccgcgcccca caagagctgg tcgccgacgg tgaacgcgct
     1861 gatgtattcg ccgcccatac ccagtttgcg gatgcgtccg acggggacgg acagcgtgcc
     1921 ggtaactttg gcgggggtca gctcgcggat gctggcttct ttttcgttgg ggatgacttt
     1981 cacccagtcg ttcgcgcctg ccaaaatcgc ttcgatttcg gaaatgggca ggtctttttt
     2041 caacttcagg gtaagggctt ggctgtggca gcgcatagag ccgatgcgga cgcacaggcc
     2101 gtcaatcacg gttggattgt cgctgcggcc gaggattttg ttggtttccg cgccgccttt
     2161 ccattcttct ttggactggc cgttgcccaa atccacgtca atccacggaa tcaggctgcc
     2221 ggcgagcggt acgccgaagt tggctttcgg atagtcttcg ctgcgcagga aatcagatac
     2281 tttgcggtcg atgtcgagaa tcgagccggc aggatcggca agttcgtccg ccacttgggc
     2341 gtgaaccgcg cccataccgc tgatgagttc gcgcatgttt ttcgcgcctg cgccggaagc
     2401 ggcttggtag gtcatgctgg ttgcccattc gaccaaatcg ttttggaaca ggccgcccaa
     2461 agccatcagc atcagggaaa cggtacagtt gccgccgatg tagtttttca cgccgttttt
     2521 gaggccgttg tcgatgacgt tgcggttgac ggggtcgagg acgataatcg cgtcgtcttt
     2581 catacgcagc gaggatgccg cgtcaatcca gtagccgttc cagccgctgt cgcgcagggc
     2641 ttggaagacg gatttggtgt agtcgccgcc ttggcaggta acgatgatgt ccatttttgc
     2701 cagctcggca acgttgttcg cgtccaataa tgttttagcc gcctgaccga aatcaggggc
     2761 tgcgccgccg acgttggaag tggtaaagaa aaacgcttcg ggaatgtggg cgaagtcgtt
     2821 ttcttctttc atacgctgca tcaaaaccga accgaccata ccgcgccagc cgacgaaacc
     2881 tactttcatt gtgttgctcc ttgaggaaaa atcgttaata aaattatgta tggcgtaaag
     2941 gaagcgtttt aacgccgtat tgggaaaatg taaagttctt tatcgggatt tctttaagta
     3001 ttggcgtttt cggtgtcgtg gcggtagaat cggggtcagt taagaacaat tatcaggaac
     3061 ggatatggaa gaaaaaaacg actacaccga cgcggcatcc gacaaccgca acgggcagga
     3121 aattgaagtc ggaattgccg gcgcaggcga ccggatactt gccgcgctgc tgaaccaatt
     3181 gtttaccttt ttggttttgt tggtgccgtt tgccggactg attgctttcg ccatcaagaa
     3241 tgaaggcagg attggcggca gtgaggaaat attcgggctg cttttgggca tgacttcctt
     3301 ttgggtagga ctggcgggca ttttggttta taccgtcgtt caaatctact atatgagtcg
     3361 ggacgggcag tcattgggta agaaaatcat gagaatcagg gtgttgaaaa ccgacggccg
     3421 caatcccggt tttgtcggca cggttttggt acgcgaaatc gcatggtcgg ttttggttgc
     3481 cattattgcc gccgttatcg gtcttgcagt aggtgacaac ggagaaaacg ccatcaacct
     3541 gctggcattc cttgccaact ttgtcctgct ctttatggtc aaacgcgatc gccgcacgct
     3601 ttacgacata ctggcggata cggttgtcgt caagctgccc aaataaacgg acacggcaaa
     3661 atgccgtctg aaagcctttc agacggcatt tttatcttca gaaccgctca agcatcgtgc
     3721 agtgaagccg cgtgcagggt gttttccatc aatgtggcaa tcgtcatcgg acccacgccg
     3781 ccgggaacgg gcgtaatcat cgccgcccgt tcttttgccg tttcaaattc cacgtcgccg
     3841 cacaggctgc cgtcgtccag acggttgatg cccacatcaa taacgaccgc gccgggtttg
     3901 atccattcgc ctttgacaaa gttcggaatg cctacgccga ccaccaaaat atcggccccg
     3961 gcaacctcgt ctgccagatt ttcggtcgca ctgtggcaga ccgttaccgt tgcgcgcgcc
     4021 agcagcaatt ccaaagcctg cgggcggccg acaatattcg acgcgccgac cacgaccgct
     4081 tttttcccct tcggatcgat gccgtaagcc tccaaaagcg tcatcacgcc cttgggcgta
     4141 cacgggcgca ttagcggcat tttgactgca agcctgccca cattgtaagg atggaagccg
     4201 tccacgtcct tatccggcga aatacgttcc aaaaccgcct ggctgtcgag gtgcttcggc
     4261 agtggcagct gaaccagaat accgtccact tcggaatcga cattcaggcg gtcgaccagt
     4321 gccagcagtt cttcctgcga tgttgattcg ggcagctcgt aagacagtga tttgatgccg
     4381 catttttggc aggaggtttt cttgttgcgg acataaaccg cgctggcagg gtcgcctccg
     4441 accaaaacca cggccaggca aggcgtgtgc agattgttct gttggcgttg cgccaccgct
     4501 tcggcaaccg cctgcaggcg tttttgcgaa acttctttac cattgatcag ttgtgccgac
     4561 atatccgtcc ttttgttaaa caggtagaaa atgctgaaaa ttatcgcatt ttccgtttgt
     4621 ttttacagca ggatgccgaa aataaatttg ccctgaaata aaatacggct gtttggacgg
     4681 attaaatatt ttttcaaatc aaaaaactat atttttcatc ttggtgtctt gaaaataaat
     4741 gttgacgcat ctttccctga gttgtatagt tcgcttcttc aagtcggggc gtagcgcagc
     4801 ccggttagcg catctgcttt gggagcagag ggtcgtgagt tcgaatccca ccgccccgac
     4861 caatcaatca gcacgaatcg gcatgagcgc ccgtagctca accggataga gcaccgacct
     4921 tctaagtcgg gggttacagg ttcgattcct gtcgggcgtg ccaagaattt gattgaaaac
     4981 cgtttttatg gtggctgtag ctcagttggt agagccccgg attgtgattc cggttgtcgt
     5041 gggttcgagc cccatcagcc accccagata aaggcccgta cttttcggta cgggtctttt
     5101 cccgtttgcg cgtactgtcc gcgccgccgg ctttgccttt cgcatatgct tctgtatcat
     5161 gtgggttttt ctgtctttta tggtttttgt cggaggcttt gatggttgac gcttggtctg
     5221 tccctgattt tgaatcgaaa atctgtccgc ccgaggcatt gtccgaacgg ttggctcggt
     5281 tgccgcgccc gctggtgttt accaacggct gtttcgacat cctccacagg gggcacgtta
     5341 cttatctggc gcaggcgcgt tcggcagggg cggcgttggt gctggcgttg aataccgatg
     5401 cttcggtgcg gcgtttgggc aaggacggcg accgcccgat taatcctttg gagaaccgtg
     5461 ccgccgttgc cgccgctttg gaaagtgtgg atttggtaac gtggtttgac ggggatacgc
     5521 cggcggcgtt gattgaggcg gtcaaacctg agattttggt caagggcggc gattgggctg
     5581 cggataagat tgtcggtgcg gcagaaacgt tggcgcgcgg cggtcaggtg ttttcaattc
     5641 cgtttctgca ccagacttcg acaacgaaga ctttggcaaa aatccgtgcg gcagagggcg
     5701 gaaaatgacg gttttgaagc tttcgcactg gcgggtgttg gcggagcttg ccgacggttt
     5761 gccgcaacac gtctcgcaac tggcgcgtat ggcggatatg aagccgcagc agctcaacgg
     5821 tttttggcag cagatgccgg cgcacatacg cgggctgttg cgccaacacg acggctattg
     5881 gcggctggtg cgcccattgg cggttttcga tgccgaaggt ttgcgcgagc tgggggaaag
     5941 gtcgggtttt cagacggcat tgaagcacga gtgcgcgtcc agcaacgacg agatactgga
     6001 attggcgcgg attgcgccgg acaaggcgca caaaaccata tgtgtgaccc acctgcaaag
     6061 taagggcagg gggcggcagg ggcggaagtg gtcgcaccgt ttgggcgagt gtctgatgtt
     6121 cagttttggc tgggtgtttg accggccgca gtatgagttg ggttcgctgt cgcctgttgc
     6181 ggcagtggcg tgtcggcgcg ccttgtcgcg tttaggtttg gatgtgcaga ttaagtggcc
     6241 caatgatttg gttgtcggac gcgacaaatt gggcggcatt ctgattgaaa cggtcaggac
     6301 gggcggcaaa acggttgccg tggtcggtat cggcatcaat tttgtcctgc ccaaggaagt
     6361 agaaaatgcc gcttccgtgc aatcgctgtt tcagacggca tcgcggcggg gcaatgccga
     6421 tgccgccgtg ttgctggaaa cgctgttggc ggaacttgat gcggtgttgt tgcaatatgc
     6481 gcgggacgga tttgcgcctt ttgtggcgga atatcaggct gccaaccgcg accacggcaa
     6541 ggcggtattg ctgttgcgcg acggcgaaac cgtgttcgaa ggcacggtta aaggcgtgga
     6601 cggacaaggc gttctgcact tggaaacggc agagggcaaa cagacggtcg tcagcggcga
     6661 aatcagcctg cggtccgacg acaggccggt ttccgtgccg aagcggcggg attcggaacg
     6721 ttttctgctg ttggacggcg gcaacagccg gctcaagtgg gcgtgggtgg aaaacggcac
     6781 gttcgcaacc gtcggtagcg cgccgtaccg cgatttgtcg cctttgggcg cggagtgggc
     6841 ggaaaaggtg gatggaaatg tccgcatcgt cggttgcgcc gtgtgcggag aattcaaaaa
     6901 ggcacaagtg caggaacagc tcgcccgaaa aatcgagtgg ctgccgtctt ccgcacaggc
     6961 tttgggcata cgcaaccact accgccaccc cgaagaacac ggttccgacc gctggttcaa
     7021 cgccttgggc agccgccgct tcagccgcaa cgcctgcgtc gtcgtcagtt gcggcacggc
     7081 ggtaacggtt gacgcgctca ccgatgacgg acattatctc gggggaacca tcatgcccgg
     7141 tttccacctg atgaaagaat cgctcgccgt ccgaaccgcc aacctcaacc ggcacgccgg
     7201 taagcgttat cctttcccga ccacaacggg caatgccgtc gccagcggca tgatggatgc
     7261 ggtttgcggc tcggttatga tgatgcacgg gcgtttgaaa gaaaaaaccg gggcgggcaa
     7321 gcctgtcgat gtcatcatta ccggcggcgg cgcggcaaaa gttgccgaag ccctgccgcc
     7381 tgcatttttg gcggaaaata ccgtgcgcgt ggcggacaac ctcgtcattc acgggctgct
     7441 gaacctgatt gccgccgaag gcggggaatc ggaacatact taaaaaatac accctttttc
     7501 aacacggggc ggtatccgtc cgaatccgtc cggattctgc cgcccgacag actgccgccc
     7561 aggcgggcgg ggtttcaaac attgaggaaa ttctaatgaa atggctcttc acccttttgg
     7621 cggtactcaa tattgccgta ttcggcggca cggtgggcta taaattggcc gttaaagcga
     7681 cgggcggcgt gccggaaaac cgggccgtcg aaaacacgcc ccccgcaacg cccgccgccg
     7741 acaatgcggc ggcatccgtg gaggatacgg cggcactgct caaaccgggc gacatcctga
     7801 ccgaagagca ggccgagcag ttgcgcttga aaaaggaagc ggaacagaaa aaactgaggg
     7861 agaaaaaaca gcgcgaggaa aaagcccgcc gcgaaaaact cgccgccgaa aaggcgcagg
     7921 cggaacgcga aaacggcgcg gcggatgcct tatgcgccgc gcaggcaagc ctcacgatgg
     7981 acgaagacga ctaccaccgc atcaaagggc ttttgggcaa atggtcgcac gttgccagca
     8041 ggagcgtcga aaaacgcacc gccaaagcca aacctgccga caaaacctac cgcgtcgtcc
     8101 tgcccgtttc cgccgatgcc gaaaatcagg cggcggagct gtctgccaaa ggtttcaacc
     8161 ccataccgtt tgacggcgca ttgagtttgg gtgtcggcaa cagccgggaa aacgcccaag
     8221 ccctgcaaaa ccggcttgcc ggcgccggat tcggcggggc gcatattgtc gaacactttg
     8281 ccgaagccga caggcaggac gattctttga gcgtgtcgcg tatgacggtt ttgtttaccg
     8341 gcgtgaatgc cgccgatgcg gacgaaattc gtaaaatcac gtccctatac ggcaaactga
     8401 acctcaagtc ttgcaaatag gcgcgaaagc cggacgataa acgccaaacc caccgcgaag
     8461 gtgggtttgg cgtttcggtt ttgagtatct gatatataaa aagggaatgt atgtctcgta
     8521 tatgccgact ccaagtgtga aagtgaattg tgagggaata cagtgtttaa tggttcggaa
     8581 tggacgactt ccgagtggtt ttgattatac gctaataata ataattatca ataaaaattt
     8641 aaaaataaat gcggattttt tgtaatacga tgaaaaataa acaaattaac ctgtccgccg
     8701 atgtttgtga ttcgggtcgg caaactttca ttgtgtcgga caaatcgggg tcggcagtca
     8761 tagctgaggg cttcgtgcct gacggaaata agcctgccgt gtttgaacat ccgtccgatt
     8821 tggcgcggac agggttgtca ttcgggggtg tttgagcgtg gattcgggga tgcaaaagcg
     8881 gtaaaaccgt tgcggatgaa ggcttacgga cggctttagg tatttttaag tgtattcaaa
     8941 caacaacaaa acgcttcttc tattgccgta tttagggctt gcgcggcagc ctgcacaccg
     9001 tggaggccga gtgtttcgtt ggttgcggca tcgggacggg cggcaaggtt ttccggccgg
     9061 tttttccatt ttttccgaca gagccgtgat gcttgggaat ggcggtttgc ccgtttcaga
     9121 ccgccgccgt tttgggctgc gatgccgtct gaaccgttca gacggcattt ttgccgcctt
     9181 tttcaatatt ccgccgaatg ccaaaacggt tgtccgactg tcggcgtgct ggcttgcgct
     9241 ttgtcgcgtg cttcgaccgg tccggcttca aatgccagcc gtccggattc gacggcgagt
     9301 gcgaaggcgc gtgccatatt gaccggatcg ccgctgcggg aaacggcagt attcaaaagc
     9361 acgccgtcaa agccccattc catcacttgt gccgcctgtg agggcaaacc caagcccgcg
     9421 tcgataatca gcggcgtgtc gggcaggcgt tcgcgcagga cgttcaacgc gtaggcgtga
     9481 accgcgccca aacccgtgcc gatcggggcc gcccacggca tcaacgcctg acagcccgcg
     9541 tcgagcaggc ggcggcaggc aatcaggtct tcggtgcaat aaggcagcac tttgaagccg
     9601 tctttaatca ggatttccgc cgcttcgaca agctggaaca catccggctg caaggtgtcg
     9661 tcatctccga tgagttccaa ttttatccaa tcggtttcaa acacttcgcg cgccatttgc
     9721 gccgtcgtta ccgcttcctg cacgctttgg cagcctgccg tgttcggcag gacgggaacg
     9781 ccggtttctt gaagcagcga ccaaaacccc tgaccgtgcg cctcgccgcc gcttcccgcg
     9841 cggcgcagcg agacggtaat catcgcaggc tgggcggttt ggatggattg tttgaggatt
     9901 tcgggggtcg ggtaggcagc cgtgccgagc agcagccgtg aagggaaagt ttctccgtat
     9961 agggtgagca taatgggttc ctttgtatag tggattaaca aaaaccagta cggcgttgcc
    10021 tcgccttagc tcaaagaaaa cgattctcta aggtgctgaa gcaccaagtg aatcggttcc
    10081 gtactatccg tactgtctgc ggcttcgtcg ccttgtcctg atttaaagtt aatccaccat
    10141 aacgttgttt tggggacagg ggtcgtctga aaaggcaaaa ccgcctagcc gccgacgacg
    10201 gggcggacga tatcgatttt gtcgttttcg ttcaaaaccg tttccgcata cgcgcctttg
    10261 gggatgaaaa cggtgttgac cgccacggca aagggctgtt gcggcgcggt ttgggcgatg
    10321 aggtcggcaa cgcttgtgcc gtgaagttcg gcgggtccgc cgtttaagat gatgttcatg
    10381 gtttctcgat gtttct
//
LOCUS       DAWEWP010000059        10285 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWEWP010000059 DAWEWP010000000
VERSION     DAWEWP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.059"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(257..1474)
                     /locus_tag="WGN53_001092"
     CDS             complement(257..1474)
                     /locus_tag="WGN53_001092"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ0140091.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVEAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1676..2404)
                     /gene="rph"
                     /locus_tag="WGN53_001093"
     CDS             complement(1676..2404)
                     /gene="rph"
                     /locus_tag="WGN53_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ0140092.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2486..2950)
                     /locus_tag="WGN53_001094"
     CDS             complement(2486..2950)
                     /locus_tag="WGN53_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ0140093.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3167..3943
                     /locus_tag="WGN53_001095"
     CDS             3167..3943
                     /locus_tag="WGN53_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ0140094.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPGCQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4188..5042
                     /locus_tag="WGN53_001096"
     CDS             4188..5042
                     /locus_tag="WGN53_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ0140095.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5083..6291
                     /gene="pncB"
                     /locus_tag="WGN53_001097"
     CDS             5083..6291
                     /gene="pncB"
                     /locus_tag="WGN53_001097"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ0140096.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     AEGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AATQNPDDPPFLISDFGTRRRYNLPWQEHVIRTLLEAAPGIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVQLRNFQKAALESWVHEYRGDLGIALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYEWGDKAYTHYKKLKIDSRTKMLTFSDGLDIDRSWALHQYFKD
                     RFKTSFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNNTFLAYLRQ
                     VFGVPAVQTS"
     gene            6383..8101
                     /gene="argS"
                     /locus_tag="WGN53_001098"
     CDS             6383..8101
                     /gene="argS"
                     /locus_tag="WGN53_001098"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ0140097.1"
                     /translation="MNLHQTVEHEAVTAFAAAGIADNPIVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVAEALADNAVIESAEVAGPGFINLRLRPEFLARNIYAALNDA
                     RFGIAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVVRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPTFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQAVVDDLVQKGL
                     AVEDDGAKVVFLEEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGVDEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWNAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATVPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKSEGATRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8211..8586
                     /locus_tag="WGN53_001099"
                     /pseudo
     CDS             8211..8586
                     /locus_tag="WGN53_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8684..10102
                     /locus_tag="WGN53_001100"
     CDS             8684..10102
                     /locus_tag="WGN53_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0140098.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMNLIGAINL
                     VPFILTAPALYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHLAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGMPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFSGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPKSD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2380 a         3003 c         2579 g         2323 t
ORIGIN      
        1 cagtacggcg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc
       61 aagtgaatca gttccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt
      121 tgttaatcca ctataaattt aatccactat attttttgtt ccaaagtcaa aatatgccgt
      181 ccgaacattc gggcggcaga caaaacggca ctgcccgata aaggcagtgc cgttgtccgt
      241 ttcaaaccgt gaaacatcag cccaaattaa aggctttatg caataccctg gttgccagtt
      301 ccatgtattt ttcatcaatc aatacggaaa ctttgatttc ggaggtggaa atcatttgga
      361 tgttgatacc ctcttcggcg agcgtgcgga agattttggc ggctacaccg acgtgcgaac
      421 gcatacccaa accgactgcg gagactttgc acacggtgtc gtcgccgtcg atagaagccg
      481 cgccgatact gtcttgacgt tccgacagga tttccaaagt ctgcttgtaa tcgccgcgcg
      541 gtacggtaaa ggaaaaatcg gttgtgcctt cgctgccgac attttggata atcatatcga
      601 cttcgatgtt ggcatcggca accgcgccta aaatctgata ggcgacgccg ggtttgtcgg
      661 gtacgccgcg cacgttgatg cgggcttggt ttttgtcgaa tgcgataccg gttacggcag
      721 ctctttccat attgtcgtcc tcttcaaagg taattaaggt gccgttgccg ccgtcttgca
      781 ggctgctcag tacgcgcagg cgcactttgt attttccggc aaattctact gaacggattt
      841 gcaaaacttt cgagccaagg ctggccagct caatcatctc ttcaaacgtt accgtatcca
      901 tgcggcgcgc ttcgggtacg acgcgggggt cggtggtgta aacgccgtcc acgtcggtat
      961 agatttggca ttcgtctgct ttgagggctg cggcgagcgc aacggcggag gtgtcggagc
     1021 cgccgcgtcc gagcgtggaa atgtcgcctt cgctgctgat gccttggaaa ccggcgacga
     1081 tgacgacttt gccggcggca aggtcggcac gcattttttc gtcatcaatg ctttcgatgc
     1141 gggctttggt gtgggcggta tcggttttga gggcgacctg ccagccggtg tagcttttgg
     1201 cttccacgcc gatgtctttc aatgccatcg ccaaaaggcc gatggttact tgttcgccgg
     1261 tggacaaaac cacgtccaat tcgcgcggat cgggatgctc ctgcatttcg tgcgccaacg
     1321 cgaccaggcg gttggtttca ccgctcatgg cggatacgac gacgacgatg tcgtgtcctt
     1381 cggcgcgggc tttggcgaca cgtttggcca cgtttttaat gcgttcgggc gagcctacgg
     1441 atgtgccgcc gtatttatgt acgattaacg ccatgtttcg tgctttcttg tgtgggattg
     1501 gtcgggcagc atggtttgct ggaaaagcgg ctattattac tattttttac atgaagttca
     1561 agaacggact gcgctttccc gcctgccgtt tgacagcggt cagcgaaaaa cctgttcttt
     1621 cagattgttg acaaaatgcc gtctgaacgg ttttcagacg gcatccggac gacaatcagg
     1681 cggcggacaa cgcattttgc tggtgttgca gcagttcgcc tatgcctttt tgcgccagtg
     1741 caaccagttt gcccaattcg tccaaactga acggcgcgtc ttccgccgta ccttggattt
     1801 cgatgatttt tcccgatgcg gtcatgacga tattcacatc actgtcgcaa ccggagtctt
     1861 cgggataatc caaatccaaa agcggcacgc cgttcactac gcctactgac acagcggcaa
     1921 cggcttcgcg gatggggttt tcactcaaaa tgccgtctga aaccagtttg ccgacggcga
     1981 tttgcagcgc gacaaacgca ccggtaatcg aagccgtgcg cgtaccgcca tctgcctgaa
     2041 tcacatcgca gtcaatcaag atttgtcgtt cgccgagttt ttccatatct acgaccgcgc
     2101 gtagcgaacg cccgataagg cgttggattt cttgtgtgcg cccggactgt ttgcccgccg
     2161 aagcttcgcg gcgcatccgg gaagcagttg aggcaggcag catcccgtat tccgccgtta
     2221 cccagccttg gtttttaccg cgcagaaacg gcgggacgtt ttcatctatg gaagcggtac
     2281 aaatcacttt ggtattgccg cattcaataa ggcacgaacc gtccgtatgc ggcaggaaat
     2341 gaggggtgat tttgatatcg cgcaggctgt ccgcggcgcg cgagatgcgg atgtaatcag
     2401 gcatactgcc ctcccgttaa aaacagataa attaaaaagc cttaaatatg aaaaatcaca
     2461 tttaaggcct tcaaactgaa aatttctacg cctcttcggc tttgctgcgg ataatcaaaa
     2521 gcggcaggtg gctttggcgc attaccgttt cggcaaaact gcccattaaa aggtgcatca
     2581 gtccggtacg tccgtgcgta cccaacacca gcaggtcggc accgttttca tcggcataat
     2641 caaccaaatc ctgcgccatt tcacgcgcac ccttattggc aaccagcagg tgtttgacgg
     2701 tattttccac acccagttcc cgggcggtgc gctcggcggc atccaaaact tcgttgcctt
     2761 gcgcgacggc ggcggcttcg tagctttcgt gttgcaaaaa ttcgggggcg agcgccatat
     2821 attcggcagg attggcaacg tgtaccaaag tcagacgcgc accgttgacc ccggcaagct
     2881 cggcggcatg tttcagggca ttgatggacg tttcactgcc gtcaacggca acaaccagat
     2941 gtttgtacat atcgtattct ccttttgcac cgcctcgcgg tgccctcttg tcggatgggc
     3001 gcagggacag tttgcgccgt ttcattatag acccgctgtc gggctttata caacagccga
     3061 acagcccgac cgctttccag tataatatgc cgcttccgtg cagtcaggca ttttttgccg
     3121 gctttcgttc actttttgat ttgacgcaat cttgcaggat tcgaccatgt ccgacaacgc
     3181 tttgacctct tcgcgacgct tcggcggcat cgccagactc tacggagact ctgccttggc
     3241 gcacttttca caggcacacg tctgcgtagt cggcgtgggc ggtgtcggct cgtgggcggt
     3301 cgaggctttg gcgcggacgg gcatcggacg tttgactttg attgatttgg acaacgttgc
     3361 cgaatcgaat gtcaaccgcc agctgcacgc cctgaccggc gacttcggca aagcaaaagt
     3421 taccgccttg cgcgaacgcg tcgcccaaat caacccagga tgccaagtgt ttgaaattga
     3481 agatttcgtt accgaagaca atttgccgga atacttcgga aaaggttttg atttcgtcat
     3541 cgacgcgatc gaccaagtgc gcgtcaaagc agcaatggcg gcttattttg tggaacgcaa
     3601 acaaccgttt gtcctcagcg gcggcgcggg cggacaaaaa aatccggcgt taatccaaac
     3661 cgccgatttg agccgcgtaa cccacgaccc gctgcttgcc aacctgcgct acaccttgcg
     3721 gaaacgctac ggattcagcc gcgatacgaa agcaaatatg cgcgtgcctt gcgtgtattc
     3781 gaccgaaaat atcgtgccgc cgcagtctag ggaggcttgt tcggcagatg ccgctccgca
     3841 aggcttgtcg tgcgccggct acggtgcaag catgctcgtt accgcttcgt tcgggctata
     3901 ttgcgcacag gcggcggtgg aacacatcgc agacaaaaaa taagcaatgc cgtctgaaac
     3961 aggattcaga cggcatttga acaaacgcgg caatcaagct gtcggttcag aaaaaccaac
     4021 atcggcacgc ctaaaaccgt attcacaacc tgctcctttt caaaacattt gcatttaaaa
     4081 gccgttataa tgccgtctga acatctgccc gaccacatta tgcgtgaatg tcggcagatt
     4141 gttttctttt gtaaacttat attaaaatcc acttaccgat tcacgccatg ccgcccatcc
     4201 ctgccccatc cgcaccatcc gagcacactg tcgcatgggt attcggccaa cccgttaccg
     4261 atttgccgca ggatttgttt atcccgcccg acgcgctgaa agtcgtattg ggcagttttc
     4321 aaggcccttt ggatttgctg ctctacctta tccgcaagca gaacatcgat gttctcgata
     4381 ttccgatggt gaagattacc gagcagtatc tgcactacat cgcccaaata gaagcctatc
     4441 agtttgattt ggcggcggaa tacctgctta tggcggccat gctgattgaa atcaaatcgc
     4501 gcctgctgct gccgcgtacc gaaaccgtcg aagacgaaga agccgacccg cgtgccgagt
     4561 tggtgcgccg cctgctggct tacgaacaga tgaagctggc ggcgcagggt ttggacgcgc
     4621 tgccccgagc cggacgggat ttcgcgtggg cttacctgcc gctggaaatt gccgtcgaag
     4681 ccaagctgcc cgaagtctat attaccgact tgacgcaagc gtggctgggt attttgtctc
     4741 gggcaaaaca cacgcgcagc cacgaagtaa tcaaagaaac catctccgtg cgcgcgcaaa
     4801 tgacggcaat cctgcgccgt ttgaacggac acgggatatg caggtttcac gacctgttca
     4861 atcccgaaca gggcgcggct tacgtggtcg tcaacttcat cgcactgttg gagcttgcca
     4921 aagaaggttt ggtcggaatc gtacaggaag tcggtttcgg agaaatccga atcagcctca
     4981 atcatgaggg ggcacattca gacggcattt ccggcacacg gggcgggcgc gatgtgttct
     5041 aatacgcccc aagccgccac caaaaatcgg gagacacgcc atatgaccgg catcatacat
     5101 tcgctgcttg acaccgacct ctacaaattc actatgctgc aagtggttct gcaccagttt
     5161 ccgcagacgc acagccttta cgaattccgc tgccgcaacg cctcgaccgt ctatccgctt
     5221 gccgacatca aggaagactt ggaagccgaa ctcgacgcgc tctgccggct gcgcttcacc
     5281 cacgacgaac tcggctatct gcgctccctg cgtttcatca aaagcgactt tgtcgattat
     5341 ctcgaactct tccagctcca acgccgcttt gtcgaagtcg gcaccgatgc cgaaggccgt
     5401 ctgaacatcc gtatcgaagg ccccatgatt caagcgatgt tctttgaaat cttcatcctt
     5461 gccattgtca acgaacttta cttccgccgc ttggaaaccc ctgcagtcat agaagaaggt
     5521 gaacgtcggc ttcaagccaa agccgcgcgt ctgaaagaaa tagccgccac acagaatccg
     5581 gacgatccgc ccttcctgat ttccgacttc ggcacacgcc gccgctacaa cctcccatgg
     5641 caggaacacg tcatccgcac cctgcttgaa gccgcccccg gcatcgtacg cgggaccagc
     5701 aacgtttatc tcgccaaaaa actcggcatt acccccatcg gcaccatggc gcacgagttc
     5761 ctgcaggcat tccaggccct cgacgtacaa ttgcggaatt tccaaaaagc cgcgctcgaa
     5821 agctgggtac acgaataccg cggcgacctc ggtatcgcat tgaccgacgt ggtcggcatg
     5881 gatgccttcc tgcgcgattt cgacctttat ttcgccaagc tcttcgacgg tttgcggcat
     5941 gatagcggag acccctacga atggggggac aaagcctaca cgcactataa aaaactcaaa
     6001 atcgacagcc ggaccaaaat gctgaccttc tccgacggac tggatatcga ccgatcttgg
     6061 gctttgcacc aatatttcaa agaccgcttc aaaaccagtt tcggcatagg caccaacctg
     6121 accaacgata tggggcatac gcctttgaat atcgtgttga aactggtcga atgcaacggt
     6181 cagtccgtcg ccaagctctc cgattctccc ggaaaaacca tgaccaacaa caatactttc
     6241 cttgcctacc tgaggcaggt attcggcgtt cccgccgtac aaacatccta gtccccggca
     6301 acaatcccac aattcctatt gctgccgtac aaattctttt aaaataccga ctgatttgaa
     6361 tttaaccgaa agaccgaact tcatgaacct acaccaaacc gtcgaacacg aagccgtcac
     6421 cgcctttgcc gccgcaggca tcgccgacaa ccctattgtt ttgcagccga ccaaaaacgc
     6481 cgaacacggc gatttccaaa ttaacggcgt gatgggtgcg gcgaaaaaag ccaaacaaaa
     6541 tccgcgcgaa ttggcgcaaa aggtagccga agcattagcc gacaacgccg taatcgaaag
     6601 cgcggaagta gccggtccgg gcttcatcaa cctgcgcctg cgccccgaat tccttgcccg
     6661 aaacatttat gctgccttaa acgacgcgcg tttcggcata gcaaaaaccg acaaaccgca
     6721 aaccgtcgtt atcgactatt cctcgcccaa tctggcaaaa gaaatgcacg tcggccacct
     6781 gcgttccagt atcatcggcg acagcatttc gcgcgtgttg gcatttatgg gcaacaccgt
     6841 cgtccgccaa aaccacgtcg gcgactgggg tacgcagttc ggcatgttgg tcgcttattt
     6901 ggtcgagcag caaaaagaca acgcagcgtt cgagctagcg gacttggagc agttttaccg
     6961 cgccgccaaa gtacgctttg acgaagaccc tacctttgcc gacaccgcac gcgaatacgt
     7021 tgtgaaactg caaggcggcg atgaaaccgt gttggcgttg tggaaacagt ttgtcgatat
     7081 ttcactctcg cacgcccaag ccgtttacga cacgctgggc ttgaagctgc gccctgaaga
     7141 cgtggcgggc gaatcgaaat acaacgacga tttgcaagcc gtggtcgatg atttggttca
     7201 aaaaggtcta gcggtcgaag acgacggcgc gaaagtcgta ttcttggagg aattcaaaaa
     7261 caaagaaggc gaacctgccg catttatcgt acaaaaacaa ggcggcggct tcctttatgc
     7321 ttccaccgat ttggcgtgcc tgcgctaccg cgtcggtacg cttcatgccg accgcctgct
     7381 gtacgttgtc gaccatcgcc aagccctgca cttcgagcaa ctctttacca cttctcgcaa
     7441 agcaggctat ctgccggaaa acgtcggcgc ggcatttgtc ggcttcggca cgatgatggg
     7501 caaagacggc aaaccgttca aaacgcgcag cggcgacacc gtgaaactgg ttgacctgct
     7561 gaccgaagcc gtcgagcgcg ccaccgcttt ggtgaaagaa aaaaatcccg aattgggcgt
     7621 ggatgaagcc gctaaaatcg gcaaaaccgt cggcatcggc gcggtcaaat acgccgattt
     7681 gagcaaaaac cgcaccagcg attatgtgtt cgactggaac gccatgctct cgtttgaagg
     7741 caataccgcc ccctacctgc aatatgccta cacccgcgtg caaagcgtgt tccgcaaagc
     7801 aggcgaatgg gacgcaaccg tgccaaccgt tttgaccgaa cctttggaaa aacaactggc
     7861 cgccgagctg ctgaaattcg aagacgtgct gcaaagcgtg gcggacacgg cgtatccgca
     7921 ctacctcgcc gcctacctct atcaaatcgc taccttgttc agccgcttct acgaagcctg
     7981 cccgattctg aaatcagaag gcgcaacccg caacagccgc ctgcaactgg caaaactcac
     8041 cggcgacacg ctgaaacaag gcttggattt gctgggcatt gatgtgttgg acgtaatgta
     8101 aaaccgcacc gcccgattgc ggacaacagc cccgcaatca actggcggat catcctcatc
     8161 cgaatctgaa aaaagcggcg cgatacaccg tatccgccgc ccctcccaaa atgcgaaaca
     8221 aacaaacgcc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa
     8281 gcaagcaagc aaaaaattat aacccccttc ctgccgacgc acgcactttc cgcgcggcgc
     8341 attccccttt tcccgcccct caaatccgcc ttttcttcag gcagggtttc agcccgcctc
     8401 ttttccctgt tttcctttcc ccgacacgcg tgcgcgcccc ctgccgcacc gtgccgcact
     8461 ttcgcgcccg gacggcatcg ttccgccatc cggttctctg tttcaaatac ccctgtttca
     8521 gaaagaaatg cagatgtttc aacacacagg acgacatata aagcaccgcc ctatgtgttg
     8581 ccctgatttg gaaggggtta cacccttcct aaatatagtc tgatcctgcc gccccgaagg
     8641 acagatgtcc gagtggcgaa gtttcaaccg aaaaggaaat acgatgaata ttcacgccct
     8701 gctctccaaa caatggacgc tgccgccatt cctgccgaaa cggctgctgc tgtccctgct
     8761 gatactgctg gcccccaatg cggttttttg ggttttggca ctgctgaccg ccaccgcccg
     8821 cccgattgtc aatttggact accttcccgc cgcgctgctg atcgccctgc cttggcgttt
     8881 cgttaaaatt gccggcgtat tggcgttttg gctggcggtt ttgtttgacg ggctgatgat
     8941 ggtgatccag cttttccctt ttatgaacct catcggcgcc atcaacctcg tccccttcat
     9001 cctgactgcc cccgcccttt atcagataat gaccgggctg ttgctgctgt atatgctggc
     9061 gatgccgttt gtgttgcaga aagccgccgc caaaaccgac ttccgacacc ttgccgtctg
     9121 cgccgccgtc gtggcggcag ccggctattt caccggccat ttgagttact acgaccgggg
     9181 gcggatggcc aatatcttcg gcgcaaacaa cttctattac gccaaaagtc aggcgatgct
     9241 ctacaccgtc agccagaatg ccgactttat taccgccggc ctggtcgacc ccgtcttcct
     9301 ccccttgggc aatcagcagc gtgccgccac gcatctgaac gagccgaaat ctcaaaaaat
     9361 cctctttatc gtcgccgaat cttgggggat gccggccaat cccgaacttc aaaacgccac
     9421 ttttgccaaa ctgctggcgc aaaaagaccg tttttcagtt tgggaaagcg gcagttttcc
     9481 ctttatcggc gcgacggtcg aaggcgaaat gcgcgaactg tgcgcctacg gcggtttgcg
     9541 cgggtttgca ctgcgccgcg cgcccgacga aaaatttgcc cgctgcctcc ccaaccgttt
     9601 gaaacaagaa ggttacgcca cctttgcgat gcacggcgcg ggcagttcgc tttacgaccg
     9661 cttcagctgg tatccgaggg cgggctttca agaaatcaaa accgccgaaa acctgatcgg
     9721 taaaaaaacc tgcgccattt tcagcggcgt gtgcgacagc gagctgttcg gcgaagtgtc
     9781 ggcatttttc aaaaaacacg acaagggact gttttactgg atgacgctga ccagccacgc
     9841 cgactatccc aaatccgaca ttttcaacca caggctcaaa tgcaccgaat acggcctgcc
     9901 cgccgaaacc gacctctgcc gcaatttcag cctgcacacc caatttttcg accaactggc
     9961 ggatttaatc caacgccccg aaatgaaagg cgtggaagtc attatcgtcg gcgaccatcc
    10021 gccgcccgtc ggcaacctca atgaaacctt ccgctacctc aaacaggggc acgtcgcctg
    10081 gctgcacttc aaaatcaaat aacaacaatg ccgtctgaac gcaccaacag ccttcagacg
    10141 gcattttgca gacaggccga ccccctcaag cccacttttt tcatcatttc cgataaattg
    10201 ctttgtatag tggattaaca aaaaccagta cggcgttacc tcgccttagc tcaaagagaa
    10261 cgattctcta aggtgctgaa gcacc
//
LOCUS       DAWEWP010000060        10217 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWEWP010000060 DAWEWP010000000
VERSION     DAWEWP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.060"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(69..143)
                     /locus_tag="WGN53_001101"
     tRNA            complement(69..143)
                     /locus_tag="WGN53_001101"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Glu,seq:ttc)
     gene            complement(161..237)
                     /locus_tag="WGN53_001102"
     tRNA            complement(161..237)
                     /locus_tag="WGN53_001102"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(201..203),aa:Arg,seq:acg)
     gene            complement(347..2020)
                     /gene="recN"
                     /locus_tag="WGN53_001103"
     CDS             complement(347..2020)
                     /gene="recN"
                     /locus_tag="WGN53_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ0140099.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIALLPSSPTSHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2204..2806)
                     /locus_tag="WGN53_001104"
     CDS             complement(2204..2806)
                     /locus_tag="WGN53_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ0140100.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            complement(2907..3848)
                     /gene="ylqF"
                     /locus_tag="WGN53_001105"
     CDS             complement(2907..3848)
                     /gene="ylqF"
                     /locus_tag="WGN53_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ0140101.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3929..4783)
                     /gene="rapZ"
                     /locus_tag="WGN53_001106"
     CDS             complement(3929..4783)
                     /gene="rapZ"
                     /locus_tag="WGN53_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ0140102.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYVVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4764..5726)
                     /locus_tag="WGN53_001107"
     CDS             complement(4764..5726)
                     /locus_tag="WGN53_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ0140103.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGNLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5730..6179)
                     /gene="ptsN"
                     /locus_tag="WGN53_001108"
     CDS             complement(5730..6179)
                     /gene="ptsN"
                     /locus_tag="WGN53_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ0140104.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6364..7254)
                     /gene="ubiA"
                     /locus_tag="WGN53_001109"
     CDS             complement(6364..7254)
                     /gene="ubiA"
                     /locus_tag="WGN53_001109"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ0140105.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFTANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7284..7760)
                     /locus_tag="WGN53_001110"
     CDS             complement(7284..7760)
                     /locus_tag="WGN53_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ0140106.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDRIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7775..8422)
                     /gene="bioD"
                     /locus_tag="WGN53_001111"
     CDS             complement(7775..8422)
                     /gene="bioD"
                     /locus_tag="WGN53_001111"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ0140107.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADKRRLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEIVLVEGAGGLMVPLTEKLLTIDYIQQQAYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQYSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8419..9720)
                     /gene="bioA"
                     /locus_tag="WGN53_001112"
     CDS             complement(8419..9720)
                     /gene="bioA"
                     /locus_tag="WGN53_001112"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ0140108.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLVDGMSSWWCAIHGYNHPVLNQAVETQIKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYRQVRGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPTSRFDDEWDEADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            complement(9933..10172)
                     /locus_tag="WGN53_001113"
     CDS             complement(9933..10172)
                     /locus_tag="WGN53_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140109.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEELCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVLSMTD"
BASE COUNT         2377 a         2791 c         2593 g         2456 t
ORIGIN      
        1 tatgagaaat gagaaatgag aaatgagaaa tgagaaatga ctccgatata atcggaggca
       61 ttttgaattg gcacgcccac ggggaatcga accccggtta ccgccgtgaa agggcgatgt
      121 cctaaccgct agacgatggg cgcgtattgt attttcttac tggcgcaccc ggagcgattc
      181 gaacgcccga ccctctggtt cgtagccaga tactctatcc aactgagcta cgggtgcagc
      241 acaatcactt gttcaagtga ttaagaaagc gtgataatac ggatttatct gagttctgtc
      301 tattgtttta gacttttatt tttagttatt ttttaactga ttgattttaa aataaactat
      361 ttttcgacgc aagttgcagc aattctgccg catgttgccg cgtcgtatcg gtaatgactt
      421 ctccgcccaa catacgggca acctcttcga cccgttggat ttcatccaat atactgattt
      481 cgctgacggt ttgctctccc tcgctgtgct tgcgcacccg ccagtggttt tcgccgcagg
      541 atgcgacttg cggaagatgg gtaacggcaa gcacctgatg ttttctgccc aacgcacgta
      601 atgccttgcc gaccatttca gccactcccc ctccaatacc ggtatcgacc tcatcaaaaa
      661 tcagggtggg aacttgggta tattggctgg caacaacctg taaggcaagg ctgatacgcg
      721 ccaattcgcc gccggaggca actttattca gcggacgggg cggattgcct ttgttggcgg
      781 caacttgaaa ctgtacttgc tccaagccgt gcgacgtcgg ggaagacggc agtagggcaa
      841 tgtcaaaacg cgcacctttc atggcaaggt gttgcatatg ctcggtcgtt tcgccgctca
      901 aacgctctgc cgcctgatgg cgcatggcag aaaggatgtg ggcagcttcc tgatattcgg
      961 caaaattgtg ggcaacatta tgctcgagcg cgtccaaatc ggcggcagct tgcaggcttt
     1021 gcaggcgttc ttcgatttct gccaacttgg caggcaactc ttcaggctcg atccggtatt
     1081 tccgcgccat ccccatcagc tcgcccatgc gctgttcctg tgccgccaat tcattgggat
     1141 tgatgtcgct gcgacctgcc acatcgcgca tattggcact gatttcgccc aattcggctt
     1201 cgatgcttgc caacatattc aggctctcgg caaagcgcgg ctcgatgttt tgcagattgg
     1261 ccaatagttt ttgacattga tagatatggc gttggatgcc gttgtcgccg tcaatcttgc
     1321 ttccgacttc ttcggcagcc tgcaacagct cggcagaatg ggcaaggctg tcgtggcttt
     1381 ggctgagggc ttcccattcg ccttgtttaa tgtccaactg attcagttcg ttaaactgcc
     1441 attccagacg ctcccgctcg ataatgacgg catcggcgtg ttcctgcgcc tcttggaggg
     1501 cttttttcgc attggcccaa ttttgataaa gctgcctgac ggtttccgcc tgctccctgc
     1561 tacccgcaaa tgcgtccaac aattcgcgct gggcggcttc ctgattaagc gaatgatggg
     1621 cgttttgccc gtggatgtcg ataagctggc taccgacgga tttgagttgc gccaaggtag
     1681 cggcctgatt gttgataaag ctgcggcttt tgcctttggc atcgataatg cggcggatac
     1741 tgagttcttc ttctccgtcg tttaaaagcc cctgttcata caattctgct tttaaaacag
     1801 gcaaatggga aatatcgaac aatgccgaca actgcgcttc ttttgcaccg ctgcggactt
     1861 ggctgtaatc ggctttgtcg cccaacagca gaccaatcgc atccaaagta atggacttgc
     1921 ccgcgccggt ttctccggtc aatacggtaa agccgctttg aaaatccaga ttcagatttt
     1981 caacaatgac aaaatcacgc aaagaaagtg ttagaagcat tgtgaggatt ccttagcaat
     2041 tctaccgaca aaagcaatat caactttaga tatacgaatt ataactaaga tttttttaaa
     2101 ttaaaaataa gttttcctta attttgctta gtacttgttt ttatctgctt atttgttgca
     2161 atgccgtctg aagcaatgtg cgtttcagac ggcatttgga atttcagttg ggcagggtat
     2221 cggacggtac ggcttccggc tcttcttcat ccgtaggcgg catttccatt aaatcgggta
     2281 aaaccagttc gcccagttcg gtcagcggcg gaagttcttc cagaccgtcc aagcgcaaat
     2341 cgctgagaaa agttgccgtt gtcgcccaca atgcgggttt tcccaatgtg tcccgatgtc
     2401 cgatgacttc aatccacccc cgatcctgca aggtctgcat cacgttctgc gacaccgcca
     2461 cgccgcgtat gccctcgatg tcgccgcgcg ttacgggctg ctggtaggcg ataatcgcca
     2521 gtgtttccat cacggcgcgg gagtaacgcg gcgcacgctg ttcttgcagg ctgcccagcc
     2581 gctcgaatgc cgtctgaaca atctgaaaac gccagccctc ttgcgtatgc accagttgca
     2641 acgccctatc ctgccaacgc gttttcaact gcgccaacac atcaatcagt ttgtcctgcg
     2701 acagtggcgg cacacacaat tcgcgcatag atttttcggt cagcggttcg gtttgggtca
     2761 gcagtgcggc ttcaattagt gcgtcgggag aaattttgtc ggtcatacgg gtatccgtgc
     2821 tgaaacggca tgggtttgga tatgccgtct gaaatcggtt ggagtagaga gaagctgcct
     2881 gaaaaatatt tttcagacgg cattctttat gcttccgaag gcttctgccc tttacgctct
     2941 gcttttctgg cttcgcgtat ggctttgagt tcggcttctt tctgacgggc ttttttgagc
     3001 caagtttccc attggttcgg cgtttcgagg gtgattctgc cgattttgcc ttcacggaag
     3061 tcggtgagga tgttttcggc ggctttttgg tagttgatcc gtccgccgct gaggactgcg
     3121 ccgcgttttt tggctatcca ttcgagccaa acgttttcgt cccagtggct gctggggtct
     3181 ttgtcggctt ggtagcgttc ttgcaacata gggaggtagt ggcggcggag gtagtctaaa
     3241 agttcgaggg cgacttcttc ttcgtccaac gcgttgcgtc cgactgcgcc gccggcggca
     3301 aggttgtagc cgccttcttc gacgatgatt ttcggccaca gcattccggg ggtgtcgtag
     3361 agccagaagt cgtcggcaag gaaaaggcgt tgttcggctt tggtgatgcc gggttcgttg
     3421 ccggttttgg cggatttttt gcctatcatg ccgttgatga gggtggattt gccgacgttg
     3481 ggaatgccgc aaatcaagac gcgcaagggt ttgtctatgc cttgccggtg gggaatcatc
     3541 gcgcggcagg cttgggtgat tttgccgtgt gcgccggttt cggaggaatc gagggcaatg
     3601 gcgcgcgtgt cggggcggct gttgtagtgg tcaagccaga cctttgtgcg ttcggggtcg
     3661 gcaagatctt gtttgtttag gattttaagt ttgggtttgc ctttggaaag ctgggcaagc
     3721 agggggtttt cgctggaggc gggcatacgc gcgtccagca tttcaatcac catatcaacg
     3781 ctttttgcac gctcggcgat ggcttttttc gccttgttca tatggccggg aaaccattgg
     3841 attgccatgt ctgttctttc ttttcaatat ttgaaatgcc gtctgaaacg gaggacgggg
     3901 tttcagacgg cataatggtt tgacggaatt agcggtctga caggttttgc gcctgtctgt
     3961 gccgtatcag caattcataa cgccctttca accttcgggc gagtttttcg acaacataga
     4021 cggaacggtg ctgcccgccg gtacagccga tggcgacggt aacgtagctc ctgctttcat
     4081 cctccaaacg cggcagccaa tgcgtaacaa acctttcgat gtcgtcaacc atttcctgca
     4141 caagcggctg tccgtccaaa taatcccaaa cgggcttgtc cataccggtg taaggcctca
     4201 actcgggatc gtaatacggg ttgggcagac tgcgcatatc gaacataaaa tccgcgttgt
     4261 ttggcacacc gtatttgaac ccgaaggact ccaaaatcac cagcagcccg gtacgttcga
     4321 ccttcagcca ctgccggact gcatggcgga gctgttgggc attcatcttg gaagtgtcga
     4381 tacaataggc gatttcttta agcgggaaca gccattcccg ttctttcttt aagctttcca
     4441 acaaggtcat atcctgatta ctcagagggt ggcctcgcct ggtttcggaa aaccggcgga
     4501 ccagcacggc ctcttccgcc tcgacaaaca aaacttcaac cctgtgcccc agtccgcgca
     4561 gagaggcaat ctgttcccgc gcctgtccga tgtctatgcc ggaacgcaca tcgacgctga
     4621 ccgccaattc ggtttcgtcc gcacgctcga tatgatacga caccagcgcg ggcaacattt
     4681 ccaagggcag gttgtccacg cagaaataac ccgaatcttc catttggcgc agtgcgacgg
     4741 atttgcccga accggacaac ccgctaatca ggacgatttt catgattctg ttcgttttct
     4801 ttaagctgcg tctgatggcg ttccaaaaat tcgcgcgtac tatccttacc gcgcaactga
     4861 agaatataat tgcgcaccgc cgcttcaacc aatacggcaa ggttgcgtcc gaccgcgaca
     4921 ggcagcgtta ccgaacggac gttgacgttg aggatggatt cggtttcggt acggatactc
     4981 aaacggtcga gctgcttcat atactcgtcg tccgcctcga ccaaattgat aatgagttgc
     5041 aggatttttt tggggcggat ggaagtttcg ccgaaaatat ggcggatatt gagtatcccc
     5101 aagccgcgca cttccaaaaa atcgcgcagc ataggcgaac accgcccttc cagcgtttcc
     5161 gggccgatgc ggaacagctc gaccgcatcg tcggcaatca ggctgtggcc gcgcgaaatc
     5221 agctccaacg ccaattcgct cttacccaaa cccgaatgtc cggtaatcag cacgccgatt
     5281 tcaaacacat cgagaaatac gccgtgtttg acggacgatg ccgccagggt gcgttgcagg
     5341 taaatccgca acacgtccat cagatagggg ctttcgagtt tggaagtcag cagtggaata
     5401 tcgtttttgt ggcagtagtc gcgcagtccc ggggaaaccg gcaagccgtt tgccacaatg
     5461 accaaagaca tagaaatgtc gaacaggttt ccgaactgat aacccgtttc ccccgattcg
     5521 aggcggttca gatattccga ctccgccaaa ccgaccactt ggatttggtt ggggtggatg
     5581 aaattcaggt gtccgaccaa agcgaggacg ggcttgtccg cctctacgcc gatacggttg
     5641 tccgcacccg aattgccggc ggcccaggcg agttgcagtt tgtattggtt gtcgtcaaac
     5701 agtcggcgga cagagatact gggcatattt tattcttcgg tcaggatggc gcgggcttct
     5761 tccgcagaag aaaccgtcat cagcgattcg cggatgcttt tttgggaaaa cttgccggcc
     5821 agtttggaca atacctccaa atgctcaccc gtcgcatttt ccggaaccag caagataaaa
     5881 accagggaaa ccggtttgcc gtccggtgcg tcaaatccga cgggttcgcg cgtgcggatg
     5941 aacgcgcccg tcgcctgctt cacgccggca tgacggccgt gcgggatggc aacgccctgc
     6001 cccaaaccgg tcgaaccgag tttttcacgg gcaaaaagac attcgaaaac atcagcatgg
     6061 gacaatgagg attcgcgttc caaaagcagg cccgcttcct caaacagcct ttttttactg
     6121 cccacctcca tatccaaaac aatatgggac aaaggcaaaa tttcgccgat aaggctcata
     6181 agcttctctt ttcagacatc gcaaaacaga aagattgtac cgactgccgg ggcaaatctc
     6241 aatcccgcat acggtacggg ctgacataac acagcgtttt aaaaacatat tttaacgctt
     6301 ttcggcacag atagaaatgc cgtccaaagc agtttacggc tcttcagacg gcattgccct
     6361 gccttatttc gcgaaaaacg tatgggcaaa aatggcggta aaccacaccc aaccaattct
     6421 gttgtttgcc aaaaacgttt caaaacagat ttgccggacg cggcttttga tggcggcata
     6481 ttggcggtat tgcagcagca ggacgatggg gattgccgtc caatatgccc atgccgcacc
     6541 gataaccgcc cccaatactg ccatcagcag ggtaaagccg ccatgacaca gcataacggc
     6601 ggcgatgtcg taacgcccga acgtgacggc ggaggttttg atgccgattt tcaaatcgtc
     6661 ctctttgtcc gccatggcgt aaacagtgtc gtaagccagt gtccataaaa cattggcggt
     6721 aaaaagtatc catgcttcaa cggggacgct gttgcccact gcggcaaacg ccatcgggat
     6781 accgaaggaa aaggcaagcc cgagatagag ttggggaatc ggaaaaaaac gtttggtaaa
     6841 cgggtaagtc agcgcaagaa acagggcggg caggctcatc agccaagtca gatgattcag
     6901 cggaatcagg cacaatgcgg caagcaggca caaaaatgcc gtcagcagca gggcttcttt
     6961 tttcttgacc ctgccctgtg cgaacggacg gtttttggtg cgctcgacag caccgtcaaa
     7021 atcgcggtcg gcaaaatcgt tgatgacgca accggcactg cgcattaaaa acgtaccgat
     7081 tgtaaacgcc gccaataccg ccaaatcggg aatgccgtct gaagccagcc acaatgccca
     7141 gtaggtcggc cacagtaaaa gcagcgtccc aatgggcttg tccgcccgca tcaggcgcag
     7201 gtacacatcc aaacgatcgg acagtcgtaa aaataaaggg gatttaggat tcatattgcc
     7261 gcacagcttg aaaaaacggt attttatccg ataaagcgtt tcagttcggg cagaaaatac
     7321 tcggtcagca gcatttcctc gccgtgacgg gaaaaccgag aacgccgcgc ggcaaagtac
     7381 cgtccgcatc cttcgccgga aacggcaaac tcaaacgccg aacgcgcccc ttccaaatcg
     7441 gcttgaaaca gacgctcgcc caaagggcgc gtgccgcagt ccaaaatgtt ttgccaaaac
     7501 gccgaaccta tccggcattc gctccttgcc tcaacaacag ggatacggtc cagcttcaac
     7561 aaaacttcgc gcaccagcct ccctccgcat tccgtctcca attcgcccag tttcagcagt
     7621 tccaccgaaa atgtatgcgg caaggcgcgc aatgcggcgg tcagcgaccg ggctttcaat
     7681 aaccggctca tcggcaggtc gatgccgtct gaaacggcgg cgggcaagtc gggcagccat
     7741 ttcccaaata ggtgttccat atttttccca atctttatac cgcgtctgtt tttgccaact
     7801 ccatccattc cgcttcggaa aaatcggctt tcagacggca tttcaagtag ctcaggctgt
     7861 attgggcgat gtgtgcgtcg cggctgtcat gaatatggtt gaatacgagg ctgtgcaagc
     7921 gaatgccgta ttgtttgagc gcggcgaaac tgagcagggt gtgattaatg ctgccgagcc
     7981 gaccgctggt aacgaggatg acgggataag cctgttgctg aatataatca atggttaaca
     8041 gcttttccgt cagcggaacc atcaaaccgc ccgcgccttc gaccaagact atttcgtatt
     8101 gtgccgccaa ttcttgcgta gcggtacgga ttttgtccaa gtccaaagcc ctgccgtcca
     8161 gacgggcggc gagatggggc gaggcgggat agctgaagat ttcaggcata gtcagccgcc
     8221 gtttgtcggc ttcctgcatg ggtatgccca tgattttgcg gtgaacggcg atgtcttcgg
     8281 cgatgtcttg gcaaccggtt tgcacgggct tttgcgtaat cacgcttttg ccctgctgca
     8341 acaattgttt tgccaacata ccggtggcga cagttttgcc gatgtccgtg tctatgccgc
     8401 tgacgaagta aacgcctttc atttgctgtg ttccttcaag atttgcacgg ttttgtcggc
     8461 aagtttggtc aaaacgccgt ctgaaataat atacggcggc atcagatata ccagcctgcc
     8521 gaacgggcgc acccaaatgc cctgcgccac gcaatctgct tgaaaacgcg ccatatccac
     8581 gcctttatcc agctcgatca cgccgatggc acccaaaacg cgtacgtctt tcacgccgcg
     8641 aatgtcccac gcggctttca gacggccttt taagatgctt tcaatgcggc ggatatttgc
     8701 ctgccagtct tgagacaaaa gcagtttgac cgaagcgcag gcaacggcac acgccagcgg
     8761 gtttgccata aacgtcgggc cgtgcataaa cacgcccgct tcgccgcgcg aaatcgtttc
     8821 ggtaactttt tgcgaagtga ttgctgccgc cagcgtcata tagccgccgc tcaaaccctt
     8881 gccgatgcac ataatatccg gcacgacctc cgcgtgttcg caggcaaaca tcttgcccgt
     8941 gcgcccgaat ccggtggcga tttcatcaaa aatcaaaacg atatcaaact cgtcgcacaa
     9001 atcgcgcaat ccgcgaagat actgcggatg ataaaaatac atgccgcccg cgccctgcac
     9061 gaccggctct aaaataaagg cggcaatatc cgcatgatgc acttcaaaca aggcgcggac
     9121 aggctgcaaa tccgcctcgt cccattcatc gtcgaaacgg cttgtcgggt tatcgacaaa
     9181 ataacgctgc ggcaacgcgc tgccgaaaat atggtgcatc cccgtttccg gatcgcagac
     9241 ggacatcgcg ttccaagtat cgccgtgata cccgcgccgc accgtcgcaa tattctgctt
     9301 cgccgtcaac ccccgcacct gccggtattg caccgccatt ttcaacgcaa cttccaccga
     9361 aaccgaacct gaatccgcat aaaaaatacg gtccaacccc tgcggcaaaa tcccaaccaa
     9421 caatttaccc agctccaccg caggctcatg cgtcaaaccg ccgaacatca catgcgacat
     9481 ttgtttaatc tgcgtctcaa ccgcctgatt caaaacagga tgattgtaac cgtgtatcgc
     9541 acaccaccag gacgacatcc cgtcaaccag ccgcgtgccg tccgccaatt cgataaacac
     9601 cccttctgca cgtttgacag gataaacggg aagcggatcg gtcatggaag tatagggatg
     9661 gagcagatgg gtacggtcga aatcaagtaa tgatgatgtg tgttgatgtt cagacggcat
     9721 aagtttctct cttttcttat tgctgcattc aaacgcaaac gcgtattcta ctccgacaga
     9781 ccgtttccca cacctctcca tccgtttcgg gcgcaaaacc gcgaaacaaa tcgtccgcag
     9841 tataagcgca caccgtttcg cattccccaa gcccgattgg aatcagacga cccaacgccc
     9901 aatattgttt cacacagcct ccgcccaacg cttcaatcag tcatagacaa aacggcaatc
     9961 catacgatac agatcatagc aacagccatc gcaacagcgt tagcaaaatc aggggactcc
    10021 gacataggcg catagcatct accgatgcac agctcctcat tcggctctat gaataccata
    10081 cccatcacaa aatccaccgc caaaaccagg cacggcttct tatacttatg atagatttcc
    10141 accatcctgc cccatatatc ccaaacattc ataccgcata tcccgcaggc aacaaattcc
    10201 gattgaaggt tacagcc
//
LOCUS       DAWEWP010000061        10138 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWEWP010000061 DAWEWP010000000
VERSION     DAWEWP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.061"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WGN53_001114"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WGN53_001114"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ0140110.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDEKLAFAKESGADLVINAA
                     KEDAAKVIQEKTGGAHVAIVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAESLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1457..1846)
                     /locus_tag="WGN53_001115"
     CDS             complement(1457..1846)
                     /locus_tag="WGN53_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0140111.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNKYFNVTLSKVSPDHFTLQADPNPTTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2072..3250
                     /locus_tag="WGN53_001116"
     CDS             2072..3250
                     /locus_tag="WGN53_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ0140112.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITEAVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATAKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3316..5250
                     /locus_tag="WGN53_001117"
     CDS             3316..5250
                     /locus_tag="WGN53_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ0140113.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5553..6353)
                     /locus_tag="WGN53_001118"
     CDS             complement(5553..6353)
                     /locus_tag="WGN53_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ0140114.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAEPAVKAKS
                     AGKSVAASLKARLEKTYSAQDLKVLSVNETPIKGIYEVVVSGKQIIYTDAKGDYMLVG
                     DLINVNTRQSLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRL
                     EHEFEKMTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSI
                     CDNPVAETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6492..8681
                     /locus_tag="WGN53_001119"
     CDS             6492..8681
                     /locus_tag="WGN53_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ0140115.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSVRTAFVEEKPLPQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYHGQASHSEFVLNANQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLSAMSGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCSDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPVPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            complement(8728..9801)
                     /locus_tag="WGN53_001120"
     CDS             complement(8728..9801)
                     /locus_tag="WGN53_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0140116.1"
                     /translation="MRPFCVGATVLAILGALVFFISPGVVVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPATTLMAVLLLVAAVLLPFLPQLAAFFVAAYWLVLLLFCAW
                     LIWLARNTDNFALLMLLAAFTVFQTAYAATGDLNLLRAQVHLNMAAVMFVSIRVSVLL
                     GAEALKECRLKDPVFIPNIVYKNIAITFLLLHAAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRTVLMNVNPIFFITVPAILTVA
                     VFVLYLFTFVPIFQANAFTGDPE"
     gene            complement(9849..>10138)
                     /locus_tag="WGN53_001121"
     CDS             complement(9849..>10138)
                     /locus_tag="WGN53_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0140117.1"
                     /translation="LAGIAPHPRESGETKFKSRCFGGRSAVRKALYMATVAATRFEPL
                     IRDFYQRLLSEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
BASE COUNT         2389 a         2620 c         2919 g         2210 t
ORIGIN      
        1 gacgtgttgc gatacgggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtatcgcca
      121 cgtcaaatca gtggtgatga ccgcagccgc attctttttt catatcgatc accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggcacaacc aaactttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagtccga tggcaaccac acgtccgccc gcgcggacgc
      421 aattcacggc agagttgaat gcggcggcgg atacggcggt aacaatggct acgtgtgcac
      481 cgccggtttt ttcctgaatc actttggcgg catcttcttt ggcagcgttg atgactaaat
      541 ctgcgccgct ttctttggca aaagccagtt tttcatcgtt gatgtcgatg gcgacgacgt
      601 gtgcgccaaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacac tggaaacttt aatggcttta taagtggtta
      721 caccggcaca agtaatgctc gaagcttgcg caggatccaa gccttctggg actttgaccg
      781 cgtaatcggc actcacgata cagtgggtcg ccatgccgcc gtcggcagta tagccagcgt
      841 tcaatacaga gcggcacagg gtttcgcggc cggtattgca gtattcgcaa gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtattccaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catctcacag
     1261 gtcaagtggt atgttgttga aaaatataga ttatatgtta tattataaca tcttggaaag
     1321 gcacggcatc ggggcggttg ccggatgagg ggcggcaggt ttcaagtttg aaaaaccgga
     1381 cggcaaaccc gtaaaaatgc cgtctgaagc tgtgtccgga cggcatcttt acgggtttgc
     1441 gggcttcggc ggaggattag tcgaagccgg ggcaggattg gtttgtaccg gaagcggcaa
     1501 tggtaccgcc gtcgttgagc gtaacgacgc aggtttcgcc gtcgttggtg gtgggattgg
     1561 ggtcggcctg aagggtaaag tggtcggggc tgactttgct taaggtaacg ttgaaatatt
     1621 tgttttgttt cagtttgttt ttgtcgtagg ttttaaacgt ccctttttgg cggtagtaac
     1681 gttccatggt ctgcgcgttg tgcagcaggg tcgtcctgac ttccgacagg cggacgcgcc
     1741 ggatgtaggt tttataggaa gggtaggtga tgagcgtcag gatgccgagg atggcgacgg
     1801 caatcatcag ctcgagcagc gtaaagcctt tttgaacgtt tttcatagca atgtgtttcc
     1861 atttgtttgt cggtcggcga cattataacg ccgtatcgga aatggcggaa tatgtaaacg
     1921 gattgaaatt ttcgggaaag cagattgtat aagccattta aaacaaatgg ttatttttat
     1981 tgtcggcagt ttgccgcctt ggatggggca gggacttgcg gtagaatccg cttccgattt
     2041 atgggattga cgcatacaga gaattgaaaa catggcaaaa atgatgaaat gggcggctgt
     2101 tgcggcggtc gcggcggcag cggtttgggg cggatggtct tatctgaagc ccgagccgca
     2161 ggctgcttat attacggaag cggtcaggcg cggcgacatc agccggacgg tttctgcaac
     2221 aggggagatt tcgccgtcca acctggtatc ggtcggcgcg caggcatcgg ggcagattaa
     2281 gaaactttat gtcaaactcg ggcaacaggt taaaaagggc gatttgattg cggaaatcaa
     2341 ttcgacctcg cagaccaata cgctcaatac ggaaaaatcc aagttggaaa cgtatcaggc
     2401 gaagctggtg tcggcacaga ttgcattggg cagcgcggag aagaaatata agcgtcaggc
     2461 ggcgttgtgg aaggatgatg cgaccgctaa agaagatttg gaaagcgcac aggatgcgct
     2521 tgccgccgcc aaagccaatg ttgccgagct gaaggctcta atcagacaga gcaaaatttc
     2581 catcaatacc gccgagtcgg aattgggcta cacgcgcatt accgcaacga tggacggcac
     2641 ggtggtggcg attctcgtgg aagaggggca gactgtgaac gcggcgcagt ctacgccgac
     2701 gattgtccaa ttggcgaatc tggatatgat gttgaacaaa atgcagattg ccgagggcga
     2761 tattaccaag gtgaaggcgg ggcaggatat ttcgtttacg attttgtccg aaccggatac
     2821 gccgattaag gcgaagctcg acagcgtcga ccccgggctg accacgatgt cgtcgggcgg
     2881 ttacaacagc agtacggata cggcttccaa tgcggtctac tattatgccc gttcgtttgt
     2941 gccgaatccg gacggcaaac tcgccacggg gatgacgacg cagaatacgg ttgaaatcga
     3001 cggcgtgaaa aatgtgctga ttattccgtc gctgaccgtg aaaaatcgcg gcggcaaggc
     3061 gtttgtgcgc gtgttgggtg cggacggcaa ggcggcggaa cgcgaaatcc ggaccggtat
     3121 gagagacagt atgaataccg aagtaaaaag cgggttgaaa gagggggaca aagtggtcat
     3181 ctccgaaata accgccgccg agcaacagga aagcggcgaa cgcgccctag gcggcccgcc
     3241 gcgccgataa acgaatatgc cgtctgaaca cggaaacggt ttcagacggc atttgttatt
     3301 gatttacgga atattatgag cttgatcgaa tgtaaaaaca tcaaccgcta tttcggcagc
     3361 ggcgagaacc gcgtccatat tttgaaagac atcagcctgt cgatagagaa gggcgatttt
     3421 gtcgccatca tcgggcagtc cggttcgggc aagtccacgc tgatgaacat actcggttgt
     3481 ttggataccg ccggttccgg ttcgtaccga atcgacggca tcgaaactgc caaaatgcag
     3541 cctgacgaac tggcggcatt gcgccgcgaa cgcttcggct ttatcttcca acgctacaac
     3601 ctcttaagct cgctgaccgc aagggataac gtcgcgctgc cagccgtcta tatgggcgcg
     3661 ggcggcaaag agcgttccgc gcgggcggac aaactcttgc aggatttggg tttggcaagc
     3721 aaagagggca acaagcccgg cgaactctcg ggcggacagc agcagcgcgt ctccatcgcc
     3781 cgcgccctga tgaacggcgg agaaatcatc ttcgccgacg agccgaccgg cgcactcgat
     3841 accgccagcg gcaaaaacgt gatggaaatc atccacaagc tgcacgaagc cgggcatacc
     3901 gtcattatgg tcacgcacga ccccggcatc gccgccaatg ccaaccgcgt catcgaaatc
     3961 cgggacggcg aaatcatttc cgacacctcg aaaaatcccg aaatccccgc aagcaatgtc
     4021 gggaggattc gggaaaaagc ctcgtggtcg ttttattacg accagtttgt cgaagccttc
     4081 agaatgtcgg tgcaagcagt attggcgcac aaaatgcgtt cgcttctgac gatgctcggc
     4141 atcatcatcg gtatcgcttc ggttgtctcc gtcgtcgcat tgggcaacgg ttcgcagaaa
     4201 aaaatccttg aagacatcag ttcgataggg acgaacacca tcagcatctt cccggggcgc
     4261 ggcttcggcg acaggcgcag cggcaggatt aaaaccctga ccatagacga cgcaaaaatc
     4321 atcgccaaac aaagctacgt tgcttccgcc acgcccatga cttcgagcgg cggcacgctg
     4381 acctaccgca ataccgacct gaccgcttct ttgtacggcg tgggcgaaca atatttcgac
     4441 gtgcgcggac tgaagctgga aacggggcgg ctgtttgacg aaaacgatgt gaaagaagac
     4501 gcgcaggtcg tcgtcatcga ccaaaatgtc aaagacaaac tctttgcgga ctcggatccg
     4561 ttgggtaaaa ccattttgtt caggaaacgc cccttgaccg tcatcggcgt gatgaaaaaa
     4621 gacgaaaacg ctttcggcaa ttccgacgtg ctgatgcttt ggtcgcccta tacgacggtg
     4681 atgcaccaaa tcacaggcga gagccacacc aactccatca ccgtcaaaat caaagacaat
     4741 gccaataccc aggttgccga aaaagggctg accgatctgc tcaaagcgcg gcacggcacg
     4801 gaagatttct tcatgaacaa cagcgacagc atcaggcaga tagtcgaaag caccaccggt
     4861 acgatgaagc tgctgatttc ctccatcgcc ctgatttcat tggtagtcgg cggcatcggc
     4921 gtgatgaaca tcatgctggt gtccgttacc gagcgcacca aagaaatcgg catacggatg
     4981 gcaatcggcg cgcggcgcgg caatattttg cagcagtttt tgattgaggc ggtgttaatc
     5041 tgcgtcatcg gcggtttggt cggcgtgggt ttgtccgccg ccgtcagcct cgtgttcaat
     5101 cattttgtaa ccgacttccc gatggacatt tccgccatgt ccgtcatcgg cgcggtcgcc
     5161 tgttcgaccg gaatcggcat cgcgttcggc tttatgcctg ccaataaagc agccaaactc
     5221 aatccgatag acgcattggc acaggattga ggtttgaaca aagatgccgt ttgaagctgc
     5281 cggaccggtc attttggagc agaaacttat tggacaaaaa cggtttctta gattctacgt
     5341 tccagattcc cacttgcgtg ggaatgacgg cggtgtgttt cgtattgttc agtttattat
     5401 tttcaatcat tcaatgggtt aggatgtatt tgttggcttg ctgtactggt ttaaatttaa
     5461 tccactataa atttcagtgc ggattggttt tcaggcggca tttcccgcaa aaaaggcttg
     5521 gaattttcca agccttttgt tttgcggatg gattattgat ttttgcggat gatttcctcc
     5581 agttggggca tcgggctgta gccgctttgg ctgcgcccgt tggggaagac gagggtcggc
     5641 gtgccgttga agccgaattg ttcgcccaag gaagtggttt ccgcgacggg attgtcgcag
     5701 atgctgccgc cgaccgggaa tttgccttta cgcatccaat ccgtccacgc tttggcgcgg
     5761 tcgggctgac accataagat ttgcgccttg cgcgcggcat cagggtgcag gccggcaatg
     5821 ggcatcataa agctgtaaac cgtcacgtcg gtcatttttt caaactcgtg ttccaagcgt
     5881 ttgcagaacg gacaatcggg gtcggagaag acggcgactt tcagcttgcc gttgccgcgc
     5941 acttctttga tggctttgtc caaaggcagg gaagcaaagt cgattttgtt caaatcggcg
     6001 gcgcgttctt cagtcaggct ttggcgcgtg ttgacgttga tgaggtcgcc aacaagcata
     6061 tagtcgcctt tggcatcggt atagatgatt tgtttgccgc tgacgacaac ttcgtaaatg
     6121 cctttaatcg gtgtttcatt gacgctcaac actttcaaat cttgggcgga ataggttttt
     6181 tccaaacgcg ctttcaaaga ggcggcaacg gatttgccgg cggacttggc tttgacggcg
     6241 ggttcggctt tgacggcggg ttcggcgttg gcattggaaa cgggcgtttg cccgcaagcc
     6301 agcagcggga ggacggtaaa gggggtcaag attttgatta acttggtttt catataaaga
     6361 tgattgcgcg tgttggaaag gcggaattgt atcaaatctc tgttgcgcct gcattgcgcc
     6421 taggctcaat ttatcgtctg aaaatagctt ccgcctgtta aaatacgcaa aaaaatgatt
     6481 tgcctgcctg tatgatctac caccgcatcg ccgtaaacgt gccgctttca gacggccttt
     6541 tgacttattc ccattccgat ccgcttcctc caggaacgcg ggtgcttgtg cctttccgca
     6601 ataaaaccgt ggtcgggatg gtgtgggaaa cggatattgc gccggatatg gatacggcgc
     6661 ggattttgag cgttcggacg gcctttgtgg aagaaaagcc gttgcctcaa agctggcgtg
     6721 atttgttggc atttacgtcg cgttattacc actatccgac cgggcaggcg gtgtttgccg
     6781 cgctgccgca gggtttgaag gaaacgcgcg cggtggaaat gccgcagccg ccgttgtttt
     6841 atgctttgaa cgaagcgggc agggcgcaaa cgccgccgcc ggctcggttc aacaaaaaag
     6901 cggctttgtg ggatgcgctg ctgtcgggcg gaatgacgat ggcggcgttg aagcaggtga
     6961 acgcgcaggc ggcgaaatta atcgaagatt gggcggaaca gggttggatt gaaacgacgg
     7021 aagcggcgaa acctgtattg aggccgtacc acgggcaggc ttcgcactct gaatttgtgt
     7081 tgaatgccaa ccagcagaaa gcttccgatg ccattcagac ggcattggga cgcttccagc
     7141 cgtttctgct gtacggcatc accggcagcg gcaagaccga ggtgtatttt gatgcgatgg
     7201 cgaaagtgtt ggcgcagggg cggcaggtgt tgtttctgtt gcccgaaatc aacctcacgc
     7261 cgcagctttt gaagcgggtg gaaaaccgtt ttgccgacgt accgaccgcc gtgttgcaca
     7321 gccggatggc ggcaggcagg cgcacgcagg attatctgag cgcgatgtcg gggcaggcga
     7381 agctggtcat cggcacgcgg ctggcggtgt tcacgccgat ggatgatgtc gggctgattg
     7441 tggtcgatga ggaacacgac ggctcgttca aacaggacaa cgaattgcgc tatcacgccc
     7501 gcgatttggc ggtgtggcgg gcgaagcaga gcggctgccc gatcatattg ggcagtgcca
     7561 cccccagctt ggagagttgg cacaaggcgc aaagcggcgc gtaccgcctg ctgcaactga
     7621 ccgaacgcgc ccataccgcc gcgcaactgc cgcaagtgga catcctcaac gtaggccgtc
     7681 tgaaactcga caacggtttc tcgccgcaag ccttgcagct tttgaaacag aattttgaag
     7741 cgggcggcat gtcgctggtg tacctcaacc gtcgcggttt cgcgccagcg ctgttttgca
     7801 gcgactgcgg ccataccttc ggctgcccga actgctccgc caaaatggtg ctgcaccaac
     7861 gtgcccgcca actgcgctgc caccactgcg accaccgcga acccgtcccg ttcaaatgcc
     7921 ccgactgcgg caaccaagat ctgaccgccg tcggacacgg cacgcagcgc gtcgaagaaa
     7981 ccctgcgcgc cttcctgccc aaggcagccg tcgtccgtgt tgacagggac agcaccgcgc
     8041 acaaaaacga ctgggcggat ttgtaccgcc gcattgccga caacgaaatc gacattctgg
     8101 tcggcacgca gatgctcgcc aaagggcatg atttcgcgcg gctcaacctt gttatcgtgt
     8161 tgaacgccga cggcagcctg tacagcgcgg actttcgcgc cccggaaagg ctgttcgccg
     8221 agctgatgca ggtgtccggc agggcggggc gcgccgacaa acccggcaag gtgttgatac
     8281 agacccaact gcccgaacat cccgtcttcg ccgccgtcaa agcgcaggat tatgccgtgt
     8341 tcgccgaaaa cgaattgaac gagcggcaaa tgttcgcgat gccgcccttc ggtttccaga
     8401 ccgccgtccg cgccgatgcg ccgcgtgttg ccgatgcgat ggaatttctc aacgccgcca
     8461 aagaaaccct cgccccgctg ttgcccgaaa gcgtttccca gtttggcgcg gctccgatgc
     8521 tgatggtgcg ccttgccgaa cgcgaacgcg cgcaaatctt cctcgaatca acatcccgac
     8581 aagatttgca ccgagccgtg agtttgtggg tgcaggtgtt gcagcaaaac agggacggca
     8641 aaatccgatg gtcggtggat gtcgatccgc aggaggcttg attattggca atccgatgcc
     8701 gtctgaaaac cgtttcagac ggcattttta ttccggatcg cccgtaaacg catttgcctg
     8761 aaagatcggt acgaacgtaa agagataaag cacaaacacg gcgacggtca gaatcgcggg
     8821 gacggtgatg aagaatatcg ggttcacgtt cattaaaaca gtgcgcgaaa cggcagcggc
     8881 gaaaaggatg gggacggcga tgcggcagag tttggggtag tcgagcttgg taaagccgct
     8941 gtgccacagt ccggcggtca gccacaccat catcacgccg cccatcatgc ctccgagggt
     9001 aatcaggtgc aggggcgcgg aggcaggtaa gttttgcagt ttcgccgcgc ctgtccacaa
     9061 atagcctgcg gcggcaaaga gctggagcag gtaataagtg cggacgtagt gtttgcgcag
     9121 gagttcgtga tggtgcagtt cgcgcagttt ggcaagcagg ataaagccga cggcgagcgc
     9181 ggtaaaaccg gcggtttgcg cgggcagcca aagttcggcg gcggcgtgca agagcaggaa
     9241 agtaatggcg atgtttttat aaacgatatt tggaataaaa acagggtctt tcagacggca
     9301 ttctttaagg gcttccgcgc ccagcaaaac actcacacgt atggaaacaa acatcaccgc
     9361 agccatattt aaatgcactt gcgcgcgcaa caggttcaag tcgccggttg ccgcgtatgc
     9421 cgtctgaaaa acggtgaacg cggcaagcag catcagcagg gcgaagttat ccgtgttgcg
     9481 ggccagccaa atcaaccaag cgcagaacag caataaaacc agccaataag ccgctacaaa
     9541 gaatgcggca agttgcggta aaaacggcaa taaaacagcc gcaacaagca acaacaccgc
     9601 catcaaagta gtggcaggtt tcaggttgcc cgaaaaaccc gtccagtcca acaaagccgc
     9661 agtcaggaaa ccaccatatg ctgcaggcag catgagttct aagaagattt ggcggtgcag
     9721 gacgacgaca ccggggctga tgaaaaacac caacgcgccg agtatggcaa gcactgtcgc
     9781 gccgacgcaa aacgggcgca tagcaacagt atttttcacc ccgtcgggca aaaataccaa
     9841 aactcaaatc aagccgtccg gataccgttt tcggcagtat cgttttcggc aaaataatca
     9901 cgcatccggg cattcgatat cgtcagcagt ttgcgcatac atgccgtaac ggcaacctta
     9961 tacggcttac cctcggacag caggcgttgg tagaaatccc gaataagcgg ttcaaaacgt
    10021 gtcgctgcca cggtagccat atacagcgcc ttacgtaccg cagaccttcc gccgaagcag
    10081 cggcttttga atttggtttc cccgctctcc ctcgggtgcg gggcaatgcc ggccaaac
//
LOCUS       DAWEWP010000062        10099 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWEWP010000062 DAWEWP010000000
VERSION     DAWEWP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.062"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(129..464)
                     /locus_tag="WGN53_001122"
     CDS             complement(129..464)
                     /locus_tag="WGN53_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0140118.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            593..1498
                     /gene="mtrA"
                     /locus_tag="WGN53_001123"
     CDS             593..1498
                     /gene="mtrA"
                     /locus_tag="WGN53_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ0140119.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1560..2048)
                     /locus_tag="WGN53_001124"
     CDS             complement(1560..2048)
                     /locus_tag="WGN53_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0140120.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2195..3097
                     /locus_tag="WGN53_001125"
     CDS             2195..3097
                     /locus_tag="WGN53_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0140121.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3138..4268)
                     /locus_tag="WGN53_001126"
     CDS             complement(3138..4268)
                     /locus_tag="WGN53_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0140122.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4473..7097
                     /gene="alaS"
                     /locus_tag="WGN53_001127"
     CDS             4473..7097
                     /gene="alaS"
                     /locus_tag="WGN53_001127"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ0140123.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGSKKLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPAVLDSVKDWVGAKLV"
     gene            complement(7186..8208)
                     /locus_tag="WGN53_001128"
     CDS             complement(7186..8208)
                     /locus_tag="WGN53_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0140124.1"
                     /translation="MLTKKEFEKFLDEVCTVLRQEAKNAPFKSQDIFENRVRALCIAK
                     IPAYADIVINPEPVPQIFPDVPIGEYGVEVKFTLKDTWRSVANSIQEKNKADGVQHIY
                     VVFGKMGGIPDVKWQLYEDSVIHVRTSHVPRFEVEIESDRPSLFEGFGISYAEFADLD
                     MHEKMEYIRKYARNRLKEGERLWWIEDIDAVDSHDLPLEVRLYTSLPQEEKIRLRAEA
                     ALVSPRIVCSGRAKHKYDNAVLYLITYRGVLCHQARDLFSAGSVANSAEDYRGGNYVE
                     KSLKLIEHEIEKAALEMDDALIVEYWGESVPTEKRLEYWIKMLDDLADGWIPSESLFN
                     GRYKAK"
     gene            complement(8262..9413)
                     /locus_tag="WGN53_001129"
     CDS             complement(8262..9413)
                     /locus_tag="WGN53_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0140125.1"
                     /translation="MTEFARKNWNKLAGDAIKAKMHEHGIGFIQLSELFLEQGESETA
                     NAICNKVNRGTFSHAFYLKCMELMTKQEKQEDREKKYKIISLFSGCGGMDLGFLGGFE
                     FLNRTYDKHDFDIIWANEIDEHAVRTYRHNLGNHIVHGDIWQHLDEIPTSADIIIGGF
                     PCQDISINGKGAGISGSRSGLYKAMLSAVERVRPKIFVAENVKGLLMKHHRKDLETVL
                     SDFRNLGYQVDYYLLNAADYGVPQTRERVIIVGRLPEIPEIQIEKTNTSNSYVTARQA
                     IGDLVDKVEDEIANHIWSRAEKSPDQGSRKLDAERAGYTIRAECHGNIQWHYELPRRI
                     SMREAARIQSFPDNFIFQAKLRATERQIGNAVPPVLGWHIAAAVSKALK"
BASE COUNT         2505 a         2674 c         2391 g         2529 t
ORIGIN      
        1 cttacccctt gtttctcaaa tgccgtctga aaaaacggct gcatatgttg tctacaaaat
       61 atcgggaagc ataacaggca aaatgccgtc tgaaatattt tcagacggca tttcttatcc
      121 gaaacggatt atttttgcgt ttcaaccgct tccaatgcac gcagggcata agtgtaagcg
      181 gcaccggcat tcagggcgat ggcggttgcc aatgcgcctg cgatttcgct gtcggtcgca
      241 ccggctttgg cggcagcggc ggcgtgaacg ctgatgcagc tctcgcaacg agtggtaatg
      301 gcaacggcga tggcaatcag ttcgcgcgtt ttggcatcaa gtgcttcggc ggcggcggcc
      361 tgttccaatg cgccgtaggc ctgcagcatt ttaggatgcg ccttacccag ctcgccgaac
      421 gattttttaa ccaatgcggt atgttctttc caatctttaa acattttctt ttcctttctc
      481 ttgcgtttaa ccctgatacg cgcttgcgta tctgttttcg atgtgcgtat tattgcaatt
      541 attcagttgt gtttctcgtt taatcatctc attttatggt tcaaaaagat ttatggacat
      601 tctggacaaa ctggtcgatt tcgcccaatt gacgggcagt gtggatgtgc agtgcctttt
      661 gggcggacaa tggtcggtac ggcatgaaac cttgcaacgc gaaggattgg tacacattgt
      721 tacatcgggc agcggctatc tctgcatcga cggcgaaact tccccgcgtc cggtcagtac
      781 aggggatatt gtatttttcc cgcgcggctt gggtcatgtg ttgagccacg acggaaaatg
      841 cggagaaagt ttacaaccgg atatgcggca gcacggtgcg tttacggtca agcagtgcgg
      901 caacggacag gatatgagcc tgttttgcgc ccgtttccgc tacgacaccc acgccgattt
      961 gatgaacggg ctgcctgaaa ccgtttttct gaacattgcc catccgagtt tacagtatgt
     1021 ggtttcaatg ctgcaactgg aaagcaaaaa acctttgacg gggacggttt ccgtggtcaa
     1081 cgcattgtcg tctgtcctgc tggtgcttat cctgcgcgcc tatctcgaac aggataagga
     1141 tgtcgaactc tcgggcgtat tgaaaggttg gcaggacaaa cgtttgggac atttaatcca
     1201 aaaggtgata gacaaaccgg aagacgaatg gaatgtcgac aaaatggtgg cggctgccaa
     1261 tatgtcgcgc gcgcaactga tgcgccgttt caaaagccgg gtcggactca gcccgcacgc
     1321 ctttgtgaac catatccgcc tgcaaaaagg cgcgttgctg ctgaaaaaaa acccggattc
     1381 ggttttgtcg gtcgcactgt cggtaggctt tcagtcggaa acgcacttcg gcaaggcgtt
     1441 caaacggcaa tatcacgttt cgccgggtca ataccggaaa gaaggcgggc aaaaataaat
     1501 cggggcttca aacgcaaatg ccgtctgaaa aggctttcat acagcatttg cgtaccgcgt
     1561 catttcaagg gctgcatctt catcacttcc atcaaaaagt tggtaaatgc ggggttgttg
     1621 ggtttgacat ccatattttt ccaacgctgc tgccagccgc gcaaggcatt ctggatatac
     1681 agcttggact gttccgtatt gattgcgccc cgctggctgt ctatcgccga acgcaggtag
     1741 atttcataca tactgtcatc gacggcattg cgtccgacca ggcgttttct gaagttgttc
     1801 agatattgcg ccgcctgaac cttggtcatt ttaccgatac ccacctgata gcccaagcgc
     1861 gtcgcttcat cgctgatttt ggcaacatcc gtccaatgcg aagaggcaag gcggaaacct
     1921 tttgcaggtg cttccgtttt gacggtattg ataggattca cggggatttc cgtcaatgtg
     1981 ggcacataaa tagactggca gccggaaaga actgccacaa tggaaagagg gataaggtat
     2041 tttttcatgc ccccattata atcaagtttg ccttgagaaa acaaattgtt cggcaagaaa
     2101 aataaaattt cggcatcaga agcaggcaaa aacacattcc acaagccttg ccgcaaggtt
     2161 tacaatccga ccgtccttat cgcaacgacc gtttatggat accgcaaaaa aagacatttt
     2221 aggatcgggc tggatgctgg tggcggcggc ctgctttacc attatgaacg tattgattaa
     2281 agaggcatcg gcaaaatttg ccctcggcag cggcgaattg gtcttttggc gcatgctgtt
     2341 ttcaaccgtt gcgctcgggg ctgccgccgt attgcgtcgg gacaccttcc gcacgcccca
     2401 ttggaaaaac cacttaaacc gcagtatggt cgggacgggg gcgatgctgc tgctgtttta
     2461 cgcggtaacg catctgcctt tggccaccgg cgttaccctg agttacacct cgtcgatttt
     2521 tttggcggta ttttccttcc tgattttgaa agaacggatt tccgtttaca cgcaggcggt
     2581 gctgctcctt ggttttgccg gcgtggtatt gctgcttaat ccctcgttcc gcagcggtca
     2641 ggaaacggcg gcactcgccg ggctggcggg cggcgcgatg tccggctggg cgtatttgaa
     2701 agtgcgcgaa ctgtctttgg cgggcgaacc ctgctggcgc gtcgtgtttt acctttccgt
     2761 gacaggtgtg gcgatgtcgt cggtttgggc gacgctgacc ggctggcaca ccctgtcctt
     2821 tccatcggca gtttatctgt catgcatcgg cgtgtccgcg ctgattgccc aactgtcgat
     2881 gacgcgcgcc tacaaagtcg gcgacaaatt cacggttgcc tcgctttcct atatgaccgt
     2941 cgttttttcc gccctgtctg ccgcattttt tctgggcgaa gagcttttct ggcaggaaat
     3001 actcggtatg tgcatcatca tcctcagcgg cattttgagc agcatccgcc ccactgcctt
     3061 caaacagcgg ctgcaatccc tgttccgcca aagataaaaa atgccgtccg aacatccttc
     3121 agacggcata tcgggcttta tttccccgcc ttcacatcct gccactggcg caccataaac
     3181 ttcaatgccg ccggctggat aggcaccatg ataaagctgt ttttcaaatc ctcctcggtt
     3241 gggaaaatcg tattgtcgtt tttaaattcg tcttccatca gctcacgcgc cggcttgctc
     3301 gaaggcgcat aagtaacgaa attgccgttt ttcgccgaca cttccgggtc gaggaagtcg
     3361 ttgatgtatt tgtgcgcgtt ggcgacgttt ttcgcatctt tcggaatcac gaaagaatcc
     3421 acccaaatcc ccacgccctc tttgggcatc atcacgcgga ttttttcctt gccgcccgct
     3481 tcttcggcac ggcgtttggc gatgttcaaa tcgccgccga aaccgattgt tacgcaggta
     3541 tcgccgcgcg ccaaatcatc gataaagccg gacgaagtaa agcgtttgat attggggcgg
     3601 tttttcttga gcagggcggt tgcctccctg atgtcttccg tattgctgct gttcgggttt
     3661 ttacccaaat agttcaacac cataggatag atttccgccg cgctgtctaa ataactgata
     3721 ccgcattgtt tgagtttgga cgtgtattcg gggtcgaaca ccaaatccca ctggttgtcc
     3781 ggcagcttgt ccgtacccaa agcctttttc acgcgttcgg tattgatggc gaaggtattt
     3841 gtcccccaat aaaacggcac ggcgtattcg tggccgggat cgaccccgtc catcagcctc
     3901 atcatttcgg gattgaggtg tttataattg ggaatcagcg atttatcgat tttctgatac
     3961 gcacctgcct taatctgcct gcccacaaac gcattggacg gcgcgacaat gtcgtaaccg
     4021 gattttccgg tcagcacctt gctttccagc gtttcatcgc tgtcgtacac atcataagta
     4081 accttgatgc cgtttttctt ttcaaaatcg gcaacggttt ccggatcgac gtattccgac
     4141 cagttgtaaa ttttcaatac gtttcggttt tccgccggcg ccggtttttc ggcaggcggt
     4201 ttgtccgaac cgccgcacgc tgcaagcagc aaagcagtca ggacggcgag tggcagatgt
     4261 ttggccatta tcattccttg catatcgggt tggagaaagc ggtcattata gccgatattg
     4321 gcaacagggc ttcagacggc attcaaaatc ccgccacacc cttccgaaaa ccgccgcttc
     4381 catagctaga aacagggatt tgcggtaaga taccgccgtt cgttttccct gcttttacca
     4441 tgacaagaca tttgagagac attgaaaaaa ttatgaaaac ctccgaactg cgccaaaaat
     4501 tcctaaaatt ttttgaaacc aaaggccaca ccgtcgtccg ctcttccagc ctcgtgccgc
     4561 acgacgaccc gaccctgctg tttaccaacg cgggcatgaa ccagtttaaa gacgtattct
     4621 taggtttcga caaacgcccg tacagccgcg ccaccaccgc gcaaaaatgc gtacgcgcag
     4681 gcggcaagca caacgacttg gaaaacgtcg gctacaccgc ccgccaccac accttctttg
     4741 aaatgatggg caacttctcc ttcggcgact acttcaagcg cgacgcgatc cacttcgctt
     4801 gggaatttct gacttccccc gaatggctca atatccccaa agacaaactg ttggcgaccg
     4861 tttacgcgga agacgacgaa gcctacaaca tctggttgaa cgaaatcggt atgccgtccg
     4921 agcgcatcgt ccgcatcggc gacaacaaag gcgcgaaata cgcgtccgac aacttctggc
     4981 aaatgggcga caccggccct tgcggcccct gctccgaaat tttctacgac cacggcgaag
     5041 aaatttgggg cggcattccc ggcagccccg aagaagacgg cgaccgctgg atcgaaattt
     5101 ggaactgcgt attcatgcag ttcaaccgcg acgaacaagg caatatgaat ccgctgccca
     5161 agccgtccgt cgataccggc atgggcttgg aacgcatggc cgccgttatg cagcatgttc
     5221 acagcaacta cgaaatcgac ttgttccaag acctgctcaa agccgttgcc cgcgaaaccg
     5281 gcgcagcttt cagcatggaa gaaccaagcc taaaagtcat cgccgaccac atccgctcct
     5341 gctcattcct gattgcagac ggcgtattgc cttccaacga aggccgcggc tacgtattgc
     5401 gccgcattat ccgccgcgcc gtgcgccacg gttacaaact gggtcaaagc aaaccgttct
     5461 tccacaaact ggtggccgat ttggtcaaag agatgggcga tgcctaccct gaattgaaag
     5521 aaaaacaagt tcaaatcgaa gaagcgttga aaaacgaaga aagccgtttt gcccaaactc
     5581 tggaaaccgg tatggctttg ttggaaaacg cgcttgccaa aggcagcaaa aaactcgacg
     5641 gcgaaatcat cttcaaactc tacgatacct acggtttccc atacgacttg actgccgaca
     5701 tctgccgcga acgcaatatc gaactggacg aagcaggctt cgagcgcgaa atggaagccc
     5761 aacgcgcacg cgcacgcgcc gcacaaagct tcaaagccaa cgcccaactg ccttatgacg
     5821 gtcaagacac cgagtttaaa ggttatagcg aacgccaaac cgaatccaaa gtcctcgccc
     5881 tctacaaaga cggcgagcaa gtcgacgaat tgaacgaagg cgacagcggc gcagtcgtca
     5941 tcgactttac cccgttctat gcagaatccg gcggccaagt cggcgatgtc ggctatatct
     6001 tctcaggcga aaaccgcttt gaagtacgcg atacccaaaa aatcaaagcg gccgtattcg
     6061 gtcaattcgg cgtacaaact tcaggccgtc tgaaagtcgg cgacagcgtt accgccaaag
     6121 tggacgacga aatccgcaat gccaatatgc gcaaccacag cgcaacccac ttgatgcaca
     6181 aagccctgcg cgatgtattg ggcagacacg tcgaacaaaa aggctctttg gttaccgccg
     6241 aatccacccg tttcgacatt tcccatcccc aagcggtaac tgccgaagaa atcgccgaag
     6301 tcgaacgccg cgtcaacgaa gccgttttgg cgaacgttgc cgtcaacgcc gccattatga
     6361 gcatggaaga cgcgcaaaaa accggcgcca tgatgctctt cggcgaaaaa tatggcgacg
     6421 aagtacgcgt attgcaaatg ggcggtttct ctaccgaatt gtgcggcggc acgcacgttt
     6481 cacgcaccgg cgacatcggc ctcttcaaaa tcatcagcga aggcggtatt gccgcaggcg
     6541 tgcgccgtat cgaagccatc accggcctga acgcactcaa atgggcgcaa gagcaagagc
     6601 gtttggtgaa agacatcatt gctgaaacca aagcccaaac cgaaaaagac gtactggcga
     6661 aaatccaagc aggcgcggca cacgccaaag cattggaaaa agaattggca cgcgccaaag
     6721 ccgaactcgc cgtccacgca ggcgcaaaac tcttggacga cgcaaaagac ttgggcgcag
     6781 ccaaactcgt tgccgcccaa atcgaagccg acgcagccgc cctgcgcgaa atcgtaaccg
     6841 acctgaccgg aaaatccgac aacgccgtga ttcttttagc ggcagtaaac gacggcaaag
     6901 tctccctgtg cgccggcgta tccaaagccc tgaccggcaa agtcaaagca ggcgatctgg
     6961 ttaaatttgc agccgaacaa gtcggcggca aaggaggcgg cagaccagat ttggcacagg
     7021 cagggggcac ggatgccggc aaattgcccg ccgtgttgga tagcgtgaaa gactgggtcg
     7081 gcgcgaagct ggtttgatgt gggaaaggca gcctgaaagg gattcaggct gccttttgtg
     7141 ttaaagatgc cgtctgaaag atattttcag acggcctctg tcagtttatt tggctttata
     7201 cctaccattg aatagcgatt cagaaggtat ccagccatct gccaaatcat ccaacatctt
     7261 aatccaatac tctaggcgtt tttctgtcgg gacgctttct ccccaatatt cgacaattag
     7321 agcatcatcc atttccaaag cagctttttc aatttcatgc tcaatcaatt ttaaagattt
     7381 ttctacatag tttccgccac ggtagtcttc cgccgagttg gcaacgctgc ccgcactgaa
     7441 taagtcccgc gcctgatgac aaagcacacc gcgataggta atcagataca ggacggcatt
     7501 atcgtatttg tgcttcgccc tgcctgagca gacaatacgg ggggagacta atgctgcttc
     7561 agctcggagg cggatttttt cttcctgcgg cagggaagtg taaaggcgca cttccaaagg
     7621 cagatcatgg ctgtctactg cgtcaatgtc ttctatccac cataaccgct cgccttcttt
     7681 cagcctgtta cgggcatatt ttcggatata ttccattttt tcatgcatat ccaaatcggc
     7741 aaattcagca taggaaatac cgaaaccctc aaacagagac ggtcgatcag attctatttc
     7801 tacctcaaag cgcggaacat gcgatgtgcg gacatgaatt acgctatctt cgtaaagttg
     7861 ccatttcaca tcgggaatgc cccccatttt tccgaatacc acatagatat gctgtacacc
     7921 gtcagctttg tttttctctt ggatactgtt tgctacgctg cgccaagtat cttttagcgt
     7981 gaatttgact tctacaccat attcccctat tggaacatcg gggaaaattt gcggcacagg
     8041 ttcaggattg ataactatgt cagcataagc gggaattttt gcaatgcaca gcgcacgaac
     8101 tctgttttca aaaatatctt gggatttgaa aggtgcgttt tttgcctctt gcctcaatac
     8161 ggtgcaaacc tcgtctaaaa acttctcaaa ctcctttttt gtcagcataa tgcattacca
     8221 cttaaaaata taaagtagta attttatctt atttaacagg gttatttaag tgccttacta
     8281 acggcggcgg cgatgtgcca ccctaaaaca ggagggacgg cgttgccaat ttgccgttct
     8341 gtggcacgca attttgcctg gaaaataaaa ttgtcgggaa acgattggat gcgcgccgcc
     8401 tcacgcatgg agatgcggcg gggtaattcg taatgccact gtatgtttcc atggcattct
     8461 gccctaatag tgtagcccgc cctttcggca tccaattttc gactaccttg atcggggctt
     8521 ttttctgctc tactccaaat atggttggca atttcgtctt ctactttgtc gaccaaatcg
     8581 cctatagcct gtctagctgt tacatagctg ttgctggtat ttgttttttc aatctgtatt
     8641 tcgggaattt caggtagcct tccaacgatg ataactcgct cccgagtctg tggaacaccg
     8701 taatcggctg cattcagcaa gtagtaatct acctgataac ccaaattgcg aaaatctgaa
     8761 agcactgttt ccaaatcttt ccgatgatgc ttcatcagta gccctttgac attttcagca
     8821 acaaatattt tggggcgaac gcgttcaaca gcagacaaca tggctttata cagcccgctg
     8881 cggctgcctg aaatacctgc acctttacca ttaatagaaa tatcctgaca aggaaagcca
     8941 ccgataatga tgtctgcgct agtgggtatt tcatccaaat gctgccaaat atcaccatgt
     9001 acaatgtgat ttcctaagtt gtggcggtag gttctaacag cgtgttcgtc gatttcgttc
     9061 gcccaaataa tatcaaaatc atgtttgtca taagtacggt ttaaaaactc aaagcccccc
     9121 agaaaaccca aatccattcc gccacaaccg ctaaataaag aaataatttt atattttttt
     9181 tcgcggtctt cttgcttttc ctgtttggtc attaactcca tacatttaag ataaaaagca
     9241 tgggaaaaag tacctctatt tactttgtta caaattgcat ttgccgtttc actttcccct
     9301 tgctccaaga ataattctga taattgtata aagccaattc catgctcatg cattttggct
     9361 tttatggcat ctccagctaa cttgttccaa ttttttcgtg caaattcggt catggttaat
     9421 ctttcagtca ttcataaatg acattatttt agcgtttttt ataatttaag ccaagattta
     9481 aaatctacct ttaggtttac aggccgtctg aaaacaaaaa tctcgttttc agacgacctt
     9541 tttccgctaa aatcccccta ccgtccaacc cgaaacaagg agaaccatca tgcaagttcc
     9601 tacttccgcc atcatcaacg gcgaatttgc cgacaaatac ggcaaacgcg gcagcaagcc
     9661 gtagccagca cccctgtcaa ccgcgtgcgt ggctagacca cacaccctac cactgcgggc
     9721 gacgcaaacc ttaagattga ggtgtagggt gtgtgcggta cgcacgcacg cggtttttgt
     9781 tactaccgaa tgattccaaa ccatgccgta atccagtcag cagcaaacgc tgcttgggtc
     9841 atgtaccaac aaaactcaat atcaaaggcc gtctgaagaa taattttcag acggcttctc
     9901 tctatcaaaa gcagcctgca cattcaaaaa tcgaagtgca ggctgctttt tgtgatgatt
     9961 ggaaaaaaca cacactattt gagacctttg caaaattccc caaaatcccc taaattccca
    10021 ccaagacatt taggggattt ctcatgagca ccttcttcca gcaaaccgcc caagccatga
    10081 ttgccagaca catcgaccg
//
LOCUS       DAWEWP010000063         9798 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWEWP010000063 DAWEWP010000000
VERSION     DAWEWP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.063"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(13..1308)
                     /locus_tag="WGN53_001130"
     CDS             complement(13..1308)
                     /locus_tag="WGN53_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0140126.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1434..2321)
                     /locus_tag="WGN53_001131"
     CDS             complement(1434..2321)
                     /locus_tag="WGN53_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0140127.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2321..3286)
                     /locus_tag="WGN53_001132"
     CDS             complement(2321..3286)
                     /locus_tag="WGN53_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0140128.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNITISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WGN53_001133"
     CDS             complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WGN53_001133"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0140129.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4622..4729)
                     /locus_tag="WGN53_001134"
                     /pseudo
     CDS             complement(4622..4729)
                     /locus_tag="WGN53_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4808..5077)
                     /gene="rpsO"
                     /locus_tag="WGN53_001135"
     CDS             complement(4808..5077)
                     /gene="rpsO"
                     /locus_tag="WGN53_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ0140130.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5300..6235)
                     /gene="secF"
                     /locus_tag="WGN53_001136"
     CDS             complement(5300..6235)
                     /gene="secF"
                     /locus_tag="WGN53_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ0140131.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTKGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6239..8092)
                     /gene="secD"
                     /locus_tag="WGN53_001137"
     CDS             complement(6239..8092)
                     /gene="secD"
                     /locus_tag="WGN53_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ0140132.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRVGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8304..8570)
                     /gene="yajC"
                     /locus_tag="WGN53_001138"
     CDS             complement(8304..8570)
                     /gene="yajC"
                     /locus_tag="WGN53_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ0140133.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8698..>9798
                     /locus_tag="WGN53_001139"
     CDS             8698..>9798
                     /locus_tag="WGN53_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ0140134.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2509 a         2585 c         2432 g         2272 t
ORIGIN      
        1 tgcctgcgac gcttaacggc tgtctttcca ctgataatat ttcgagccga ggaaagaacc
       61 gagtttgcgc aaaaacggtt gcaggataat attcggctgg cgcaaccgct caaacggctt
      121 cagacggcat tcgtccccta aaattgcttc ggcaaccgcc agacctgcaa tgcctgttat
      181 cgccatcccg tgtccggaat aaccttgtgc ataaaaaaca tttgcggcca aacgtccgaa
      241 atgcgggaca aggttggcgg taatgtcgca ctccccgccc cacgaatatt cgattttgac
      301 atcgtcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg tcatacgcgc
      361 aggattgtcg ataaactcgt tatccttacc gccgaaaagc agtctgccgt ccgcgctgag
      421 gcggtaataa tccaaaatat ggcggttgtc gcatactgcc atattgttac ggataagccc
      481 ttttgcgcgc gcccccaagg gttcggtcgc aataataaag gtgctaacgg caatcgcctt
      541 gcgttccaaa ggctggaata tcgggttcaa acctgcatag gtattgacgg catacaccag
      601 atttttgcat tcgacgctgc cttcgggggt gtaaaccagc caaccgtttt gataaggttc
      661 aatgcgtatc atgggagaat gctcaaaaat cttcgtacca gcttcggcag cggcgcgggc
      721 gatgcccaac gtgtaattga gcggatggag atgcccggac aagggatcga actgtgcgcc
      781 ttggtacata tcgctgtcaa gctgctgctt cagttcagtg ttatcccaga gttgataatg
      841 agttgcaccg taatattttt gggcgtgctc atgccattgt tgcaattctt cccaatgctg
      901 cgaacggatg gcaaccgtgg cataaccgcg ctgccaatcg caatcaatgg catgtttgcg
      961 gacgcgttcg tccaccagtt cgaccgcctg caaagactgt tgccaaaacc attgcgcttg
     1021 ctccaaaccg acctgttttt caatttcctc cataccgcag gcgtaatcgc tgataacctg
     1081 cccgccactc cgtcccgacg cgccgaaacc gatacgcgcg gcttccaaca caaccgtttc
     1141 atgtccctgc tccgccaagg gcaatgcagt gcacaaacca ctcaatccgc cgccgatgat
     1201 acaggtatcg gttttcagac ggcattgaag tttcggataa acagtatgag gattaaccga
     1261 actgaaataa taagaaggca gatattcttg aaaatcaggg cgaatcattg tgtttgcttt
     1321 atcgggtata ttttcggacg gaatgataca ggctgtcgag ccatatcgtc caaacagaaa
     1381 atcggttgaa gaaaacaggc tgacccagtc atgcggtcag cctgccttat taattaattt
     1441 gctttctcgg cagccaattt ttcttggcgg taggcttctg ccgcttctcg gtcacgcttg
     1501 gttgcctgcc tcatcatcca ataattgacg atgatgacca atgttccgat gatgccgatc
     1561 aggatggttg ccaaaacgtt catctgcgga tcaagaccca gcttgatttt ggagaaaatc
     1621 acctgcggca atgtggatga accggggccg gagaggaatg aggtaatcac caaatcatcc
     1681 aaagacaggg taatgccgag cagaaagcct gaagcgatgg caggggcaat caaaggcaaa
     1741 gtgatgacaa aaaagatttt cagcggacgc gcgcccaaat ccattgcggc ttcttcgagc
     1801 gattggtcaa gctcaaccag acgcgaacgg ataacaacgg taatgtatgc catgcacagc
     1861 gtcgtatgtc ctaagaaaat agtgaaaaag ccacgatcga agtagagatg ttgtaaccat
     1921 tcgctgccct gcaaaaatat ctgtacctga ataatcagca gcagcataga cagaccggta
     1981 atcacgtcgg gcatcaccat aggtgcggaa atcataccgg cgaacaaggt actgccgcga
     2041 aaacgtttaa tccgcgccat cgcatagcct gccagcgtgc ccaaaacgac ggcggcaagc
     2101 gaagacacaa cggcaatccg cagcgacagc caagcggctt ccaagatggt gtcgttttcc
     2161 agcaatgcgc cgtaccactt ggtcgaaaag ccgccccaaa cggttaccag cttggattcg
     2221 ttaaacgaat agatgaccaa aacaaccagc gggatataca gaaacgccag cgacagtgcc
     2281 aacatcagtt tcaagaacca agataatttg gatttctgca ttatttggct ccttcttcca
     2341 attcgcggtt ttcataatgc tgaaacaggg caatcggcac gaccagcagc gcgaccatca
     2401 cgacggcgac ggcggaagcc agcggccagt tgttttgatc gaagaacgcc tgccacaaga
     2461 ctttaccaat catcaggttt tccgaaccgc cgaccagctc gggaatgacg aactcgccga
     2521 cagcagggac gaaaaccagc atggagcctg caataatgcc ggttttcgac aaaggcaggg
     2581 taatcgtcaa gaacgatttg accggccccg cgcccaaatc ggaagccgct tcaagcaggc
     2641 ggttgtcgag tttcaccagt tgcgtgtata gcggcagaat cataaacggc agataggcgt
     2701 aaaccatcac caaattgagc gaaaaggcat tgtagaacaa atccaaaggc tcgctgataa
     2761 tacccatttt aatcaacagg ttgtttacaa taccgttatg cccgagcaga cccatccacg
     2821 catagacgcg caacaggaac gatgtccaaa agggcagcat aatggcaagc agcaaaccat
     2881 tgcggacaga aggattggca cgagaaatcg cataggcggt cggataaccg accaacaggc
     2941 aaatcactgt cgtcatcaat gcggttttga ttgaagacca gtaggtcatc agatagatat
     3001 tgctgttttc actgtcgccg aacgggttaa gggtatgcca aaaattttgg aaaatatcgg
     3061 catagttttg ataactgatg gtgatattca gacgacctaa atcttcgtcg atgctggtca
     3121 gcggcgtaaa cggcggaatg gcgatttctt gttcggcaaa gctgattttc agcacgatgg
     3181 cgaacggaat cagaaacagc accaaaagcc aaatatacgg tacggcaatc accacacgct
     3241 gccccgggcg gcggaacagt ttgtttttca gtttattaag gttcattgca ttccccttaa
     3301 atcaacggaa cagtggcgta ggctggtttt ccggccagct gatatacacg gtttcgtccc
     3361 aagttggcgg tgtaatgttg cgtacatacc agtaaggtgc aggaacttgg cttttgacga
     3421 cgcgcccgtt ggcgagtttg atatggtata tggcaaagct gcccaaatag gcgatttctt
     3481 ttaccgtgcc tttcgcccag ttgtagtcgc ccaaatattc gggtttttct ttatataaat
     3541 caatatcctc tggtcgaata ctaacccaaa ggtcctgctc gctcggacca cccaaaccgt
     3601 gatcgatgcg gacgtggttt tccaaacctt cgcattcgat aacggcatag tcggcatgat
     3661 cttcaatcac cacaccgtca aagatgttgg tttcgccgat aaactcggca gtgaagcggc
     3721 tgttgggata gtcgtacacg tcgctgggtg tgccgacttg ctgcaactga ccgtcagaca
     3781 taatggcgat gcgggtcgcc atcgtcatcg cctcttcttg gtcgtgcgta accataatac
     3841 aggttacgcc gacttgttcc agcgtattga ccaactcaag ctgggtttgt tggcgcagtt
     3901 ttttgtccaa tgcaccgagg ggctcatcca gcagtagaat tttcggacgt tttgccagac
     3961 tgcgtgccaa agcaatgcgc tgctgctgac caccagaaag ctggtgaggt ttgcgtttgg
     4021 caaatttggt catctgaacc aggcggagca tttcttcgac gcgcgcggcg atttcgcctt
     4081 taggcatttt gtcctgtttc agaccaaagg caatgttttg ttctacggtc atatgcggaa
     4141 aaagcgcgta actttggaac atcatattga tggggcggtc atagggtgca agtttggtaa
     4201 tatcctgacc atcaaggata attttcccct gattggggct ttccataccc gccagcatac
     4261 gcagcagcgt ggattttccg ctgccggaac tgcccaaaag ggcgaagatt tcgtgttgat
     4321 aaatgtccaa gtcgatgtta tcgacagcgt aattgtcacc aaactttttc accaagcctt
     4381 ggatttgcag ataaggtttg gctgaagacg cagtggttgc ggtcataatg gcaatactcc
     4441 aataaaaaaa cgagtaccgg caaaacggat tttcgaatgg gtgataaaaa gctgtttgat
     4501 tgctggcggg agtcgcaacg tctgatgccg tctgaaactc ttgtgaagcg cacgggcagc
     4561 atgaaatgga acaagattcc aaagaacttt atattatatt agtttatgcg gttttcgggc
     4621 aatatagtgg attaaattta aaccagtaca gcgttgcctt gccttgccgt actatttgta
     4681 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactataa atatggattt
     4741 gagcttgtcg gaaagcaaca gaaaagaaaa caccgcccat ttttctgggc ggtgtcggaa
     4801 agcgtaatta tttacgcaga cccaagcggg taatcaacgc gcgatacgta tcgggctggg
     4861 tacggcgcaa gtaggccagc aggcggcggc gttggctgac cattttcaac aggccgcgac
     4921 ggctgtggtg gtctttggga ttggctttga agtggggggt caggtcgttg atgcggaaag
     4981 tcaacagagc gacttgtact tcggaagagc cggtgtcgcc ttctttgcgt tggaaatctt
     5041 taacgatttg tgctttttgt tctacggtca gtgccataat gaaaactcca aaaatataag
     5101 aatccccata gggattcaga caagtttgcc aagcctgaag acaacggcaa actccctatg
     5161 cccaagatag gacaacgtgg cattatgaca caattcccgc atttctgcac aatattttaa
     5221 gaccgtggaa gtaaccgtta aaaatgccgt ctgaagcatt gtctgcttga gacggcaatg
     5281 ttcagacggc atatgcgctt caaaccacga tttcttcttt cttcttcggt tctttaccga
     5341 tattgtcgcg gctcaaaccg aacattagca gaagcgggct ggcaaccaat acggaagaat
     5401 aaatgccgaa cacgatgcca atggtcaacg ccatagaaaa gccgtgcaag gccgcaccgc
     5461 cgaacaccag catagaaaca accatcgcct cggtcgaacc gtgggtaatg atggtgcggc
     5521 tcatcgttgc ggtaatcgcg ttgtcgatga cttccggcac ggcatgtccg cgcatcgccg
     5581 gcttgcggaa gttttcacgg atacggtcga agacgacgac ggattcgttc acagaatagc
     5641 ccaatacggc aaggataccc gccaagacgg tcagcgaaaa ttcccattgg aagaaggcaa
     5701 agcagccgag aataatcacg atgtcgtgca tattggcgat aatggcagat acggcaaaac
     5761 gccattcaaa acgcatcgac aggtaaataa tgatgccgat aacgacaaaa cctaaagcca
     5821 tcaatccatt acttaccaat tcctcaccga cttgcgggcc gataaattcg acttggcgca
     5881 aggtaacgtc gggactgtct tttttcagca aatccataac ctgattggac aactgtgcgg
     5941 aagtaacacc ttctttgttc ggcaggcgga tcatgatgtg tttgttcgta cccaatgcct
     6001 gaacctgtac atcacctatt ttcagcgtat cgaggcgttc gcgcatctta ttgacatccg
     6061 caccctgctg atattggact tccattaccg taccgccggt aaattcgaca gagaaattca
     6121 ggcctttggt caccaagaag aacacggcag cgataaacgt aaccaacgaa atgaaggtcg
     6181 tcagtttgcc gtagctcata aacggaatat cgcgtttgat tttaaagagt tccatagctt
     6241 actccttacc tgccgcagtt tcggcttcag gtttccatac cgcaccgatg gaaatattct
     6301 gcaatttgcg tctgcgtccg taccacagat tgaccaacgc acggaatacg acgacggacg
     6361 aatacatcga agtcagaata cccaaacagt gtacgaccgc aaaaccgcgt accgggccgg
     6421 aaccgaatac caaaagcgcg ataccggcaa tcagcgaagt caggttggaa tcgacaatgg
     6481 tcgcccatgc gtgttggaaa ccgagattga ttgcctgctg cggcggcacg ccgacacgca
     6541 attcttcgcg gatacgttcg ttaatcaaga cgttggagtc gattgccata cccaaagtca
     6601 acgccagcgc ggccataccc ggtaacgtca acgttgcctg catagcagac aaaataccga
     6661 ttaggaacag tatgttggca ctcaatgcaa tggtagaaaa gaaacccatc agacgatagt
     6721 aaaccaccat gaatgcagca acgatggcaa aaccccataa agtcgaatgg aagccttttt
     6781 cgatgttctc cttacccaaa gacggaccga tggtacgttc ttcgacaatc tgcatcggcg
     6841 cggcaagaga accggcacgc aacagcaaag acgtatcatt ggcttcggct gtcgtcatgc
     6901 ttccggaaat ttccacgcgt ccgccggtaa tggcagtacg gataaccggc gcggttacaa
     6961 cctcggattt tccttggtcg atcaaaacca tcgccatgcg tttgccgaca tttgcggcag
     7021 tcagttcgcc gaaaatgctg ccgcccgcgc tgtccaagct cagactgacg gcaggtgcgc
     7081 ccatttggtc gaaactcggt tgcgcatcgt tgatgttgtc gcccgtcagc tcgacctgtt
     7141 tgctgattag cagagtttcg ggacgatctc cgccgcttga aagcagctca taaccgctcg
     7201 gcacgttgcc ttccaatgcc tcgcgcaact tggcaggatc gtcctccacc atacgcaatt
     7261 ccaaagtcgc ggtacggccg atgatgtctt ttgccttggc agtatcctga acgcccggaa
     7321 gctgcacgac gatacggtct gcacctgact gctggatgac gggttcggcc acgcccaact
     7381 cgttcacacg gttgtgcagg gtagtgatgt tctgtttgac cgcatcggaa cgcactttat
     7441 tgaccgcctc ttccgaaagc gtcaagacga tattgctgcc gtctgaattc agcgttgctt
     7501 caggaaacag cttgcgcaac tgcggcagtg ccttttgcac atcacctgca tcctgcaaag
     7561 ggacggtcag gctgtttcca gcctgacgca ccgtgccgct gcggattttt tcgcggcgca
     7621 gttcgcggcg gatgtcgccc gaataacgtt caaacgtttt ctgcatcgcc gctttcatat
     7681 cgacctgcat ggtgaaatgc acgccgccgc gcaagtccaa acccaaaaac atcggattgg
     7741 ctttgatttt cgccatccat tcggggctgt ccgccaacag gttgagcgcg gtaatatacc
     7801 cttcgcccaa agtgttttcg atgacgtcgc gcgctttaag ctgcgtttcg gtgtctttga
     7861 aacgcacttt cagtgaattg tccacaacaa acatcccgtc ggtctgaata cccgcgtttt
     7921 gcagcgcggc atccactttg gattgagtct gttcgttgat gatgatggct tgtcggttgg
     7981 tcgatacctg cacggcgggt gtttcgccga ataggttggg cagcgaatac actgcggcaa
     8041 ccgcaatcgt gaacacaatc agcagatatt tccataaagg ataacggttc atcattgttc
     8101 cttaatggtt ggaaccccac cctttcggtg gtgtcggaat cgggctattt cagaagaggc
     8161 aaaaaccctt cccagccagg caagaccgga aggcggcatc ttgaatatgc cgccctgcgt
     8221 gtcggaacat ggtcaagcct tcggttggaa ttcaaaacaa agtgccgcat tcgggctttc
     8281 cagatgcggc ttgtcggcac aaatcaatcg acttttgcgg caatcgcatt gcgttctact
     8341 tcgacctcga tttttgtacc ctgtccgata tccacggtaa aaaactgttc gccgactctg
     8401 gttaccttac ccttgaaacc tgccgccaag accactttgt cgccgacttt caaggcggca
     8461 agcattgcct gatgcgcttt gaatttcttt tgctgcggac gcatgatcag gaagtagaac
     8521 accaccataa tcaacactaa aggagcaaat tgtgcaacag cttgattcat aatttatccg
     8581 ttctttctaa tatggttgaa aatcgagagg ggtatataat aacataagac cgtaaacaat
     8641 atatcgggtt tgccgtccgt accgaccgta taccgcagcc tgcccgtcca caaacccatg
     8701 tccttgaccc gcctaatctt gaaattctat gcactgttgc gccttttttt gggcaaaaac
     8761 gcccgcaccg catggatttc gcatcccgcc tgtgccgggc acgaacccgg cgcaaaccat
     8821 cccgattcgc ccgaccgcat cctctgcatc gagcaggcat tgcgccgcgc cggtatttgg
     8881 cagcacctcc aaaccataga ggcggaagaa atcagcgata cgcgcctcgc acttgtccac
     8941 tcgagcaaat atctgaaccg tttggaatct tgcctgcccc aaaaaggcaa gattttccgc
     9001 ctggataacg acactgcaat cagcacaggc tcgctgtctg ccgcacactt tgccgccggt
     9061 tcggcagttc aggcagtcga catggtcatg aaccgtaaag catggcatgc cttttgcgcc
     9121 gcccgcccgc ccggacacca tgcgggcagc ggcaaagccg gcggattctg cctgctgaac
     9181 aacgttgccg ccggcgtcat gcatgccatt gccgaatacc gcctgaaacg cattgccgtc
     9241 atcgatttcg atgtccacta cggcgacggt acggcagaaa tattcaaaga cgatccgcgc
     9301 atcctgtttt tcaacctgtt tgaaaccgac cttttcccct tccccgaaaa caacgatatg
     9361 cccgacggcg gcaatatggt gcacctgccc ttgccgccgg gaacgggcag ccgcacattc
     9421 cgcgaagccg tccgcaggca gtggctgccc cggcttgccg cattcaaacc cgaactggtg
     9481 ctgctgtcgg caggattcga cgcacaccgt ctagacgaat cgggcaggct caacctgcac
     9541 gaggcggatt ttgcctggct gacacacaaa atcattcaga cggcatcggg ctgccccggc
     9601 aaaatcgtat ccgtactcga aggcggctac acccttgaac ctctggcaca atctgctgcc
     9661 gagcatatcc gcgtgctggc ggggctgggc aaatccgatg ccgcaaccgc ctatcagaaa
     9721 acactggatc caacaaaaaa acggtttgcc aaaccgaaaa ccggacaggt gcgccaacct
     9781 acccaatccg accgttac
//
LOCUS       DAWEWP010000064         9767 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWEWP010000064 DAWEWP010000000
VERSION     DAWEWP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.064"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(313..546)
                     /locus_tag="WGN53_001140"
     CDS             complement(313..546)
                     /locus_tag="WGN53_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ0140135.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(712..912)
                     /locus_tag="WGN53_001141"
     CDS             complement(712..912)
                     /locus_tag="WGN53_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ0140136.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1084..1959)
                     /gene="xerD"
                     /locus_tag="WGN53_001142"
     CDS             complement(1084..1959)
                     /gene="xerD"
                     /locus_tag="WGN53_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ0140137.1"
                     /translation="MEEGLIDRLLETLWLDRRLNQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVGRYYTEARPALLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVQHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2011..2451)
                     /locus_tag="WGN53_001143"
     CDS             complement(2011..2451)
                     /locus_tag="WGN53_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ0140138.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2772..3710
                     /locus_tag="WGN53_001144"
     CDS             2772..3710
                     /locus_tag="WGN53_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ0140139.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3839..4132
                     /gene="hfq"
                     /locus_tag="WGN53_001145"
     CDS             3839..4132
                     /gene="hfq"
                     /locus_tag="WGN53_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ0140140.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPASTLVQVETVQQPAE"
     gene            4216..4782
                     /locus_tag="WGN53_001146"
     CDS             4216..4782
                     /locus_tag="WGN53_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0140141.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALNNTRCRLQEAGYSNVRFILDGHENLKQYIPKPLDAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAVGQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            4836..5210
                     /locus_tag="WGN53_001147"
     CDS             4836..5210
                     /locus_tag="WGN53_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ0140142.1"
                     /translation="MQYLFVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5220..5714
                     /gene="folK"
                     /locus_tag="WGN53_001148"
     CDS             5220..5714
                     /gene="folK"
                     /locus_tag="WGN53_001148"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ0140143.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDTRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDR"
     gene            complement(5839..6318)
                     /locus_tag="WGN53_001149"
     CDS             complement(5839..6318)
                     /locus_tag="WGN53_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ0140144.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDCKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVFQI"
     gene            complement(6538..7275)
                     /gene="ubiE"
                     /locus_tag="WGN53_001150"
     CDS             complement(6538..7275)
                     /gene="ubiE"
                     /locus_tag="WGN53_001150"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ0140145.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7318..7686)
                     /locus_tag="WGN53_001151"
     CDS             complement(7318..7686)
                     /locus_tag="WGN53_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ0140146.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7806..9425
                     /gene="mnmC"
                     /locus_tag="WGN53_001152"
     CDS             7806..9425
                     /gene="mnmC"
                     /locus_tag="WGN53_001152"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ0140147.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLREHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSPR
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMQQTYAKLALDKNYRIDAPCPYLPNAYANTAHGTRGLATAPICAAAVAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(9422..9571)
                     /locus_tag="WGN53_001153"
     CDS             complement(9422..9571)
                     /locus_tag="WGN53_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140148.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT         2602 a         2981 c         1968 g         2216 t
ORIGIN      
        1 ctaccgatta cacaaacaca tatcatcttt acacaatcat gcttccatca acagtaaaac
       61 atgatatgat tgccaacaat aacatctcac aataaatttt ctaattttta ttgaaaaaat
      121 caataaataa gaattctccc aaccgacaaa taaaataaag aaagggttaa tatgcaacat
      181 cgaagaagac tagcaattta ccaagcatcc aaacgtgctt cctttaccgg caggtcatcc
      241 gccccgcaaa aacgtaaaga acgttgattt gaaaaaaatg ccgcctgaag tcctgcttca
      301 gacggcattt ttttaccgtt cgagaaactg tttcaacctg tcctcatcca aaaaccagtg
      361 gcagatttcc tcatctcccg ccaacaaaac gggaaccagc tcattgtatt tttcttccaa
      421 aacaggattt tcatccacat cgaccacttc cagcccgaac ccgtattcat tttgaaaagg
      481 tttgagtgca tcgcgcattt tgtggcacaa gctgcaatat tcacgaaaca tcaaggtcaa
      541 tttcatccgc gtttccttat ctgtcaattt gcacacgcca aagccttaga cgcagcagaa
      601 tcatggtcta tttgggaaaa aacaatgttt tcaaggaaga tgatactcaa gtcctgccaa
      661 aaacagcaaa aatgccgtct gaacagttca gacggcattt ccaaaaccgt tttacgcttg
      721 aacgttgata cccgccgtaa ccgtcggttg cgcattgtgc gccgtcagaa acgaagcggc
      781 aagctccccg cagcagccgc agcagctcgc tacgcccaat tgcgactgca aatcgcccat
      841 tgtggtcgcg ccggcggcga ttgtttcctt gatttgatgg tcggtaacgg cattgcagat
      901 gcagacaaac attttgtgct ccgtgtgttc tcaaactatc gtaccgatag cggcttttat
      961 tatcgtatgc aaatataaat aaaaacggtt cgcattgcaa ggtcggcata cacggtttgt
     1021 cttggtaatt ttttatcaag tttgcatttt cgattgattt cttattaaac cgaagcagaa
     1081 gcctcagttt cgggaatggt gttccttcac tacaccctgc aaccatacat tggcaacatg
     1141 ggtataaatc tgcgtcgtat tcaaatcggc atgtcccaac atatcctgaa ccacgcgcaa
     1201 atccaatccg tgctgcacca agtgcgtggc gaaggcatgg cgcaggctgt gcgggctgat
     1261 gtgcccgatg cctgccctac ctgcatattc tttgacaatc atccatgcca actgacggga
     1321 aatacctgtt tttttctgac tgacaaacaa ggcatcgcaa ctcctgccct tcaacaaggc
     1381 cggacgcgcc tccgtgtaat agcgtcccac ccaatacgcc gattcctgcc ccatcgggac
     1441 catcctctgc ttgtcgccct tgcccagcgc ggtaatacag cccctgtcca aatccacatt
     1501 gccgaagttc agcccgaccg cctcgctgac gcgcaagccg gtcgcgtaca ttaattcaag
     1561 cagggccttg tcccgcaaac cgtgcggcgt gtcggtatcc ggtgcggcaa gcagtcggga
     1621 aatctgctgt tctgtaatca gggtcggaat attcttgtcg attttgggcg gtttcagcaa
     1681 acgggtggga ttgtccgtcc ttatgccttc acgctccatc catatataca ggcgtttgca
     1741 tgccgataat gcgcgcgcct gcgaactccg ttgctctccg tcaacataaa ccgccgccgc
     1801 caaatccgct tcgtccgcat ccttcagcat tctgcccgat tgggacaggc ggcgggcgat
     1861 tttttccaaa tcgcgccggt aaccgtttaa agtattctga ttgagccgcc tgtccaacca
     1921 cagcgtttca agcagcctgt cgatcaaacc ttcttccata ccgttccaaa caaatgccgt
     1981 ctgaatcttc ttcagacggc atggttcaca ttatcgggaa agcgtttcca atacttcctg
     2041 cgcgtgaccc gccactttga ctttccgcca ttcatgggcg atttctccat ccttattcaa
     2101 gacgaacgta ctgcgctcga tacctaacga ctctttcccg tacagtttct tcaatttgat
     2161 gacatcaaac aggcggcaca ctgtttcatc cttgtcgctc aacagctcga accggaaacc
     2221 ctgcttggcg caaaaattct gatgcgcctt tacgccgtcg cgggaaatac cgaccacggt
     2281 ataacccaat gcctcaaact gttccaaacg cgcattgaaa tccaagcctt ccgtcgtaca
     2341 gcccggcgta ctgtctttcg gataaaaata cacgaccaaa ggcagatgtt ctgccgaatg
     2401 aaaatccgca ccgctgctcg aaggcagggt aaattcatat ttcacatcca tagtcctact
     2461 cccgatattc ccattattca aaacggcacg cagaggcacc gccgcaattg ccaaaccaac
     2521 cccgattcta ccgccccaaa ggacaaggat tcaactgccg gaaacatcca aaccgacaca
     2581 cgacggcatg aaaaatatcc atgtcaaacc acaaaatatg ttccgattta aaaacagaat
     2641 gttataaaac ccaatcccca aaacacaaca agaccgcccg cctacgggca gtctcctgtc
     2701 agacgacata ctttacagat ggctgttttt tcaacaaaat aacgccaata ctcaaaaata
     2761 tggaatcaaa aatgtccatc catactctga aacgcctgcc ctcatcgctg ctgctcggtc
     2821 tctgcctttc cctgccgtca gcccaccttt ttgccgacaa cgacatttta gggcaatttt
     2881 tagaacagaa catgcttacc tcctccgatc cgatagaaat attcgccgaa agcacaatac
     2941 accccaccaa cacccaagcc attacaggcg gtctgattct ctcctcacag tctgccctgg
     3001 tcgtcaacaa caaaaccgga cagatactgt atcagaaaaa cgccgacagg attatgccca
     3061 tcgcctccat ttccaaactg atgagcgcga tggtcgtttt ggatgcaaac ttggacatga
     3121 acgaaaccgt taccattacg cccgacgaaa tcgaccgcct caaagggacc ggcagccgtc
     3181 ttgccatagg tacggcactt acacgcaaag aactgctgca cctgagcctg atgagcagcg
     3241 aaaaccgcgc cacccatgca ttgggcagaa cctaccccgg cggcatgggc gcatttgtcg
     3301 ccgccatgaa ccgtaaagcc caaagcctcg gtatgtacgg cagccgcttt tacgaaccga
     3361 ccggactcaa cttccaaaac gtttctaccg ccaaagacct gagccttatg gtcaacgccg
     3421 ccgcccaata tccgcaaatc cgcaccaact cgacttccaa ctacgcctcg gtacagacca
     3481 aaaacgggca gcagaactac aaaaactcca atgccctggt cagagaaggc atgtggaaca
     3541 tcgaattgca gaaaaccggc tacatacgcg aagcaggcag gtctatggtt gtcaaagcca
     3601 acattcaaaa tcaacccgtt accatcgtat tgctgaactc gcccacatcc gccacacgcg
     3661 tcaacgacgc ccgcaaaatc gaatcgtgga tgctgcagca acgctcctga catacaaatg
     3721 cccggcggaa aaccgcccaa acacaaaaaa aattggtaat ttttcctatt caaggctaca
     3781 attcgacacg cacagggcgt ctgcccgatt tcacaatcat aataacggag ttaaaaatat
     3841 gacagctaaa ggacaaatgt tgcaagatcc cttcctgaac gccctgcgta aagagcacgt
     3901 tccggtttcg atttacctgg tcaacggcat caaactgcaa ggtcaggttg aatctttcga
     3961 ccaatacgtc gttcttctga gaaacacttc cgtcacccaa atggtttaca aacacgccat
     4021 ttccaccatc gtaccggcac gctccgtcaa cctacaacat gaaaacagac cccaagccgc
     4081 accggcttcg accctcgtcc aagtggaaac cgtccagcag cctgccgaat aatccgcacg
     4141 aagcatgacg tgtcatatct ttcaatacct taccgacatc ggtaaggtat ttttattttc
     4201 agacagcatt taaaaatgtt attgcaaaac atccttccat tcgcccattg ccttttgcgg
     4261 aaggcacttc ccgaaggcgg caatgcgctg gacggcaccg ccggcaacgg acacgacacc
     4321 cttttcctcg cacaaaccgc aggcatccgg gggaaagtgt gggcattcga catccagccg
     4381 caagccctga acaacacccg atgccgtctg caggaagcag gttacagcaa tgtacggttc
     4441 atcttggacg gacatgaaaa cctgaagcaa tatattccaa agccgctgga tgcagccatt
     4501 ttcaatttcg gctggctgcc cggcggggac aaaagcctta ccacccgcac ggaaaccagc
     4561 attgccgccc tttctgccac cttatcccta ctgaaagaaa acggtatgct tattgccgtc
     4621 ctctatccgg gacacgaaaa cggcaaacag gaggcagaag ccgtcggaca atgggcaaaa
     4681 aacctgcctc aagaacagtt tgccgtttta cgttacggct ttaccaaccg gaaaaacagc
     4741 ccaccctatc tgttggcatt tgaaaaactg cgtcaaaaat aactgtttgc ggtaaaataa
     4801 gccgattcca acccttattt tgataagagc cgaccatgca gtatctgttt gtcaaataca
     4861 gccaccaaat cttcgttacc atcaccattt tggtattcaa catccgtttt ttcctacttt
     4921 ggaaaaatcc agaaaagccc ttggcgggct tttggaaggc actgccacac cttaacgaca
     4981 ccatgctgct gtttacggga ttgtggctga tgaaaattac ccatttctcc ccgttcaacg
     5041 cgccttggct cggtacaaaa atcctgcttc tgctcgccta tatcgcattg ggtatgatga
     5101 tgatgcgcgc ccgtccgcgt tcgaccaagt tctacaccgt ttacctgctc gccatgtgtt
     5161 gcgtcgcctg catcgtttac cttgccaaaa ccaaagtcct gcctttctga aacaccgtta
     5221 tgaacaacag acattttgcc gtcatcgccc tgggcagtaa tcttgaaaac cctgcccaac
     5281 aggtacgcgc cgcattggac acgctgtcgt cccatcctga catccgtctt aaacaggctt
     5341 cctcactgta tatgaccgcg cccgtcggtt acgacaatca gcccgatttt gtcaatgccg
     5401 tctgcaccgt ttccaccact ctggacggca ttgccctgct tgccgaactc aaccgtatcg
     5461 aggctgattt cggacgcgaa cgcagcttcc gcaacgcgcc gcgcacattg gatttggaca
     5521 ttatcgactt tgacggcatc tccagcgacg acacccgact caccctgccg catccgcgcg
     5581 cgcacgaacg cagtttcgtc atccgccctt tggcagaaat cctccctgat tttgttttag
     5641 gaaaacacgg aaaggttgcc gaattgtcaa aacggctggg caatcaaggt atccgtcttt
     5701 tacccgacag gtaattccgc acgcggatgc cgtctgaaag cctttcagac ggcatttttc
     5761 ctttgccgcc aacacgcgtg caaaaaaatc gccccttgga aaaggggcgc aaaaggaaca
     5821 caaaccacta ccaaaacttt aaatctgaaa cactgcctgc cgcatactgt atccgacagg
     5881 atataaagcc ctcactaaat cgtttcgaga aatccaaact tcttcatcgc cgacagaaaa
     5941 tctgcctttc tccggtacca gctccaacag aaacggttga accgccgtat gcaacttgtc
     6001 cttacaccgc cccagctttc tagacaaggc ggacagggcg cgtttgtagg cattatcctt
     6061 gcagtcaagc tcccgcgcac tgccgaccca gctcaaacga aggttgcggc tttcatcctc
     6121 aatcagcaaa atgcctgttt tgacttcccc cacctcgggc tcgcatgaaa gcgcaatata
     6181 atatttgccg tcgatgttga catacgccgc ttcatgcacc tgacagttgc agccggaagt
     6241 ggcaagcagc gaataaacag agtcccgatg cagtttcaaa atatcaaggg aaatctgttt
     6301 aaaaatactt tccggcattt ttccctcctt tcgtctgctg cgttgtttac tggattgact
     6361 gcattataca gacatctccc aatcttgcaa atgataattg ttcctatttt ttaaaaaatt
     6421 tataaattat tgatggatat gtgaattaat tttaagattt aaaatctctt tctcaaaaaa
     6481 acacaaaaat gccgtctgaa acgtgttcag acggcattgg cttcacaggc agtccgttta
     6541 aaatttcacg cccttatgca gcgcgacgat gcccgcactc atattgtgat aatccacgct
     6601 gtcgaagccc gcatccagca tcatctgttt caaagtttcc tgatcggggt gcatacggat
     6661 ggattcggcg agatactggt agctgtccgc atctttggca atcagtttgc ccatgaccgg
     6721 cagcagcttg aacgaataga aatcatacgc gccttcgagc ggcttgtaga ttttggaaaa
     6781 ctccaacacc agcaacgtgc cgccaggttt caaaacacga tacatctctt tcagcgcggc
     6841 atctttatgc gtcatgttcc gcaagccgaa cgccacggaa actaagttga aataattgtc
     6901 agggaaaggc agtttttccg catcggcaag cgacacgggc aaaatcatgc cttcgttcaa
     6961 cagacggtcg cgcccgacgg tcagcataga ggaattaata tcggtcagcc aaacctcgcc
     7021 ttccttgccg acccgtttcg cccaaccgcg cgacaaatcg cccgtaccgc ccgcaatgtc
     7081 caacacttta tcgccttttt tcaggtgcgc cgtgttgatg gtgaaatgct tccacaccct
     7141 gtgcagccct gccgacatga catcattcat aatgtcgtag tttttggcga cggagtggaa
     7201 cacttccgcc actttgccgg ctttctcatc ttcgttgacc gtgctgaacc cgaaatgcgt
     7261 tttttgtcca cccatattct aaccacctcc ctgataccgc cctacggcat cccaaactta
     7321 acaatctgat tgcttttcaa aacgcgacgc gcccgccgcc gccaatttgt ccaaatattc
     7381 ctgccacatc gcatcgtatc cgtatgccag tctgtgcaga tacgcccaat cgtaaagacc
     7441 gctgtcgtgc ccgtccgaaa aactgatttt cagcgcgtac tgtccgacag gctgcaaatc
     7501 cgtaatttgc acatctgcct taccggtctg caaaacatcc tgtcccacac cgtgtccgcg
     7561 cacttccgca ctcggcgaat agacgcgtaa aaattcggca ggcagatttt taggttcgtc
     7621 cccataagtc aaaaccaaca cggtacggtt tttcaacaaa cggatttcaa caggaatatt
     7681 attcaaaaaa cacctcggtt cggcatacaa tatccggcag gcgcaggcgt gccttcaaac
     7741 ggcgcgtatt ttaacagctt tatcccgata ccgatattca aacccacact ttaaacggca
     7801 ttgatatgga caatctcgta tgggacggcg ttcccgacat ccgcacactc gaccaaacca
     7861 tccgcaaaca cgcacacccg ctcaacctga ttgtctgcct ccccgataat cagattcccg
     7921 attttcaaac cgcacaagat gcttcggacg cggaatgccg tctgaagcac cgtttggatc
     7981 aggcaacgca gtgcctccag ttcgacagca tcaacctcat cgaacacatc ctgcccgatg
     8041 tccgcttctg gctggttccc ccttcacgca cccgccgcct gcgcgaacac ttccaccaca
     8101 tttcctggca gaccgaagcc atcccgcaaa ccgaaagtaa gcccgacaaa ccctggtttg
     8161 cacttccaca aacatccgaa cggcaaaaac cggaacacat cctcgttatc ggcgcgggca
     8221 tatccggcgc ggcaaccgcc cacgccttag catcgcacgg catttccgtt accgtattgg
     8281 aagcccgaaa agccgcccaa gccgccagcg gcaaccgcca agggctgctc tacgccaaaa
     8341 tctcgccaca cgacaccgaa cagaccgaac ttttgcttgc cggctacggc tacaccaaac
     8401 gcctgctcgg acacatcctg cccgaatccg aaacctgggg cggcaacggc atcatccacc
     8461 tcaattacag ccgcaccgaa caacaacgca atcacgaatt gggtttgcaa aaacaccata
     8521 accacctcta ccgcagcatc acgcaggcag aagccgaaaa aatcgccggt atcccactgt
     8581 ccgtcccata cgaccaccct tcatgcggac tctactggca acacggcgta tggctcaatc
     8641 cacccgcatt cgtccgcacc ctcctcaacc atccgctcat tggactacac gaagacacac
     8701 cgttaaccga catttcccac gacggggaaa agtggattgc aagcacgcca aacggcatat
     8761 tttccgccac acacatcatc tactgcaccg gcgcgcacag cccccgcctg cccgaaacca
     8821 acctcgccgc cctgcctctc aggcaaatac gcggacaaac cggcctcaca ccgtccaccc
     8881 cgttttccga acaactgcgt tgcgccgttt caggcgaaag ctacatcagc ccgtcgtggc
     8941 acggactgca ctgctacggc gcgagtttta ttcccaacag cagccatacc ggatggaacg
     9001 aagccgaaga agcctcaaac cgccaagcat tggcacacct taaccccgcc ctttccgaat
     9061 cattgtttgc cgccaaccca aacccccaaa aacaccaagg gcacgccgcc atacgctgcg
     9121 acagccccga ccaccttccc ctagtcggcg cactcggcga cattgccgct atgcaacaaa
     9181 cttacgccaa actcgcgctg gacaaaaact atcgcatcga tgccccatgc ccatatctgc
     9241 ctaatgccta cgccaacacc gcccacggca cacgcgggct tgccaccgcc cccatctgcg
     9301 ccgccgccgt tgcagcccaa atcctaggct tgccccatcc cttttcacaa cgcctgcgcc
     9361 acgccctaca ccccaaccgc accatcatcc gcgccatcgt cagaaggaag gatctaaccc
     9421 cttaagcctt tgccgcatgc cgtcccgacc acagaaaatc atattcaaat aaacatacca
     9481 atcctgacgg taaaatatcg aaaacaccca taacattttc ttcccaaaat aaggagtaag
     9541 gaatgcctaa caaattgcct ttgctgtcca agatgaatgt tccgtaacat tcgaagactt
     9601 ccttaaccct gccagccgtt tcggtttctg taccccctgt tagataagga aggaatgaaa
     9661 aagccctgtt gattgtatga agtaaagggc gttaattttt gttaatcttc ccttctcagg
     9721 aactcaatat ataaggatgt taatttttat ttcccttctc gaggact
//
LOCUS       DAWEWP010000065         9678 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWEWP010000065 DAWEWP010000000
VERSION     DAWEWP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.065"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            66..260
                     /locus_tag="WGN53_001154"
     CDS             66..260
                     /locus_tag="WGN53_001154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140149.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
     gene            431..955
                     /gene="nspA"
                     /locus_tag="WGN53_001155"
     CDS             431..955
                     /gene="nspA"
                     /locus_tag="WGN53_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ0140150.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            1091..1480
                     /locus_tag="WGN53_001156"
     CDS             1091..1480
                     /locus_tag="WGN53_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0140151.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1558..2388
                     /locus_tag="WGN53_001157"
     CDS             1558..2388
                     /locus_tag="WGN53_001157"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ0140152.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2403..3860
                     /locus_tag="WGN53_001158"
     CDS             2403..3860
                     /locus_tag="WGN53_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ0140153.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3901..4290)
                     /locus_tag="WGN53_001159"
     CDS             complement(3901..4290)
                     /locus_tag="WGN53_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140154.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKGIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(4472..4873)
                     /locus_tag="WGN53_001160"
     CDS             complement(4472..4873)
                     /locus_tag="WGN53_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140155.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(5290..5619)
                     /locus_tag="WGN53_001161"
     CDS             complement(5290..5619)
                     /locus_tag="WGN53_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140156.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(5645..6352)
                     /locus_tag="WGN53_001162"
     CDS             complement(5645..6352)
                     /locus_tag="WGN53_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0140157.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITF
                     KQQPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPYDKGNQRYKDASYHGKNDNSV
                     KSRAPTNGQAALDNSVQVKSTSPRRVGVDKANNEIVVLDKTQTFNNGFAEYHGHVRSW
                     QDLHTDQKNALKKAGLD"
     gene            complement(6488..7033)
                     /locus_tag="WGN53_001163"
     CDS             complement(6488..7033)
                     /locus_tag="WGN53_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0140158.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVGNLSDMNRKPFTW"
     gene            complement(7020..7172)
                     /locus_tag="WGN53_001164"
     CDS             complement(7020..7172)
                     /locus_tag="WGN53_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140159.1"
                     /translation="MDAKSLDGKIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            complement(7154..7426)
                     /locus_tag="WGN53_001165"
     CDS             complement(7154..7426)
                     /locus_tag="WGN53_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140160.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPMGILPKPVALSIVQYEN
                     DPGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            complement(7659..7982)
                     /locus_tag="WGN53_001166"
     CDS             complement(7659..7982)
                     /locus_tag="WGN53_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ0140161.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKSMIIEFFIEGSQKNLQSIES
                     QIISYRQDFQISIEQNEFGEMWTCYCSVNIIPSLENILAIETTLFDIAQKNDCNYEGF
                     GSYGN"
     gene            complement(7985..8692)
                     /locus_tag="WGN53_001167"
     CDS             complement(7985..8692)
                     /locus_tag="WGN53_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0140162.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDEASGKTGYKPVTARYGNPYR
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGDKAETEGVWVHNECPYGEKYRTEVGSYTNTHESGRT
                     YSGKGTRQRSQISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNN
                     YNRIESPGKKYRKQDGD"
     gene            complement(8826..9143)
                     /locus_tag="WGN53_001168"
     CDS             complement(8826..9143)
                     /locus_tag="WGN53_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140163.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEERRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(9146..>9678)
                     /gene="mafB"
                     /locus_tag="WGN53_001169"
     CDS             complement(9146..>9678)
                     /gene="mafB"
                     /locus_tag="WGN53_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0140164.1"
                     /translation="DQWMQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDF
                     SDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHRNYIEGKST
                     LNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKN
                     GVHIVPANPKTIKKVQ"
BASE COUNT         2466 a         2516 c         1948 g         2748 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaacc gtatgcctgc acctgcaaca
      181 atccgacaga taccgctgtt ttttccaaac cgtttgcaag tttcacccat ccgccgcgtg
      241 atgccgccgc caccatttaa aggcaacgcg cgggttaacg gctttgccgt cggcaaagca
      301 gccggatacc gctacgtatc ttgaagtatt gaaaatatta cgatgcaaaa agaaaattta
      361 agtataatac agcaggattc tttaacggat tcttaacaat ttttctaact gaccataaag
      421 gaaccaaaat atgaaaaaag cacttgccac actgattgcc ctcgctctcc cggccgccgc
      481 actggcggaa ggcgcatccg gcttttacgt ccaagccgat gccgcacacg caaaagcctc
      541 aagctcttta ggttctgcca aaggcttcag cccgcgcatc tccgcaggct accgcatcaa
      601 cgacctccgc ttcgccgtcg attacacgcg ctacaaaaac tataaagccc catccaccga
      661 tttcaaactt tacagcatcg gcgcgtccgc catttacgac ttcgacaccc aatcgcccgt
      721 caaaccgtat ctcggcgcgc gcttgagcct caaccgcgcc tccgtcgact tgggcggcag
      781 cgacagcttc agccaaacct ccatcggcct cggcgtattg acgggcgtaa gctatgccgt
      841 taccccgaat gtcgatttgg atgccggcta ccgctacaac tacatcggca aagtcaacac
      901 tgtcaaaaac gtccgttccg gcgaactgtc cgtcggcgtg cgcgtcaaat tctgatatgc
      961 gccttattct gcaaaccgcc gagccttcgg cggttttgtt ttctgccacc gcaactacac
     1021 aagccggcgg ttttgtacga taatcccgaa tgctgcggct tctgccgccc tattttttga
     1081 ggaatccgaa atgtccaaaa ccatcatcca caccgacaaa gcccccgccg ccatcggcgc
     1141 gtacagccaa gccgtccgag caggcgacac cgtttacatg agcggtcaaa tccccctcga
     1201 tcccgccacg atgaccgtcg tcggcaacgg cgatttccgc gccgaagcgc gccaagtgtt
     1261 ccaaaacctg caagccgtcg ccgaagcggc aggcggcacg ctggccgaca tcgtcaaact
     1321 caacgcctac ctgaccgact tgggcaattt tgccgtcttc aacgaagtca tggcggaatt
     1381 tatcgccgag cctttccccg cccgcgccgc cgtcggcgtt gcctcgctgc ccaaaggcgt
     1441 gcaggtcgaa gccgaagccg tcctcgtttt gaacgcataa ttccacgttc ggactgcatg
     1501 atatttcaaa accatgccgt ccgaaccgca acactttccc acttcacaac atctgcaatg
     1561 gcacattacg caatcggcga catacaaggc tgtttcgacg aactgaccgc gctgctcggc
     1621 aaaatcggtt tcaaccacgg cacggacacc ctctggctga cgggcgacat cgtcaaccgc
     1681 ggcccgaaat ccctcgaaac gctgcaattc tgcatccggc acgaaaacag cgtgcaaatc
     1741 gtcctcggca accacgacct gcacctgctc gctgtcggct gcggcgaagg tgcgcccaaa
     1801 cgcagcgaca caatcgaacc catactcaaa caccccgacg gaaaaaaaat gctcgactgg
     1861 ctgcgtgcgc aaccgctgtt gatacgcgag ggcagccgcg tgatggtaca cgccggcatc
     1921 ctgccgcaat ggcgcataac caaagccgaa tcgctcgcta gagaagccga agccgaactg
     1981 cgcggcaaaa aatacgtcaa attcttctcc aaaatgtacg gcaacaaacc ggccgcgtgg
     2041 gacgaaggtt tgaaaggcta tgcccgcctg cgcttcatcg tcaacgcctt cacgcggatg
     2101 cgcgccctga cctttaaaaa cgaactggat ttcgactaca aatccacagt gaaaaaaatg
     2161 ccgccttacc tgcgcccgtg gttcaaagcc cccgaccggc aaaacctcga ccgcaccatc
     2221 atcttcggac actggtcctc gctgggctac acgaatgccg acaacgtcat ctcgctggac
     2281 accggcgcgc tgtggggcgg acagctgacc gccgtcaacc tcgaaacgga agaaattacc
     2341 caagtccaag ccgccggcgg catagactgg aaaagcttcg caaaataacg gaccgcccca
     2401 ctatgcacaa aatcctgccc atcgcccacg tcctctcccg actgggtatg ctgttttcct
     2461 ttatcctgct gatacccgcc gccctctcct acgccttttc ggacggcgcg tacaccgcct
     2521 tcgccaccac cgcgaccgtt accctttccg gctcgtgcat cgtccggctc gccaccctcc
     2581 ggttcaggcg cgaactgcgc ccgcgcgacg gctttaccct cgtcctgatg ttgtggctgg
     2641 cgtttgccgc tatggctgcg atgccgatgt acctgtattt cccgaatatg ggctttaccg
     2701 acgcattttt tgaatcgatg tcgggactga ccaccaccgg cgcgaccgtc atcccccacg
     2761 tcgacgggct cgccccctcc gtcaactttt ggcggcatat gctcaactgg ctgggcggga
     2821 tgggcatcat cgtccttgcc gtcgccatcc tgcctatgct cggcgtaggc ggcacgcagt
     2881 tattcaaagc cgaaatcccc ggcattgaca aagaaagcaa aatgtcgccg cgcatttccc
     2941 aagtggcgaa aaaactttgg ttcggctaca ccctgatcac catcctcgcg gcagcctgcc
     3001 tgcattttgc cgggatgggc tggttcgatg ccgtctgtca cgcgatggcg accctctcgc
     3061 tgggcgggtt ttccacccac gatgccagca tagcttatta caactccccc ctcatcgaag
     3121 cggtcatcat cgttttcacc attgtcggcg gcatcaattt tgccaaccat ttcgccgccc
     3181 tcaacagccg cagcctcaaa acctattgga aagacgaaga atgccggacg atgctgctgc
     3241 tgctctccgg cagcatcctt gccgccgccc tgtacctgtg gcacaccggc tattacgccg
     3301 gttttaccga atccctgcgc tacaccgcct tcaacttcgt ctccatcgga ctggcaaacg
     3361 ggctggccaa caccgacttc gcacaatggc cgctcctgat ttccctgtgg atgtttttcc
     3421 tcgccaacat cctcgccaac tccggctcga ccggcggcgg catcaaaacc atacgcgccc
     3481 tcgtcctgtt caaattcagc ctgcgcgaaa tgatggtgct gctgcacccc aaagccgtcc
     3541 gcaccgtcaa aatcagcggc aaggccatac ccgaccgcct cgcgctgacc gttatgtcct
     3601 tcatcttcat ctactttatg accgtcgtcc tcttcagctt cctgctgatg gcgagcggta
     3661 tggaatttac caccgccttc accgccgtca tcgcctgcat caccaacgcc ggccccggcc
     3721 tgggcgaagt cgggcccgcc ggcaattacg ccggtttgga cgtgatgcag aaatggatct
     3781 gcgttaccgc catgctcttg ggcaggctgg aaatcttcac cgtcttcatc ctcttcaccc
     3841 ccgcctactg gaagaaataa aaaatgccgt ctgaaaactt ttcagacggc atcttgtcct
     3901 ttaaagaagc ggaagctcta caaaaggaaa gaaatgctga aacctctgat aaatttcagg
     3961 atatttctct aaggctttta atgctttttc ttttgaaata ggcggatcat agacatctat
     4021 cccccttaag aaggcaatgc ccgttaatgc atttttttga tcatttgaaa tgtaaccata
     4081 attaacgact ccaattacat cataaatttc ccctataccc ttatcaaaat caatcaaatc
     4141 aaaaatatac aatggaattg tatcaatagg agtatctctg acaactatat ccaaccataa
     4201 tttaaattca gaaagattaa tagcatcata gaacatacag actattgcaa atcctaaatc
     4261 ttcacaattt tctttactaa ttttccacat aattatttac ctttatccat tgcttcccaa
     4321 ccgatacatc cggtatttta gcttctcctt tgccgtcaaa cactcctttg gttacctgct
     4381 ttgtgtcaaa aataccgtct gaaagccgaa tatcgtttca gacggcattt tgattgttgg
     4441 gtttttaatc agagctttgt ttgataattt gttattttga ataaaataca ggttctgtcg
     4501 agctgataac ttctattaca gaatcgctaa aaaagaaaat gtaagcctta taatatttct
     4561 tttcaaaaaa accaaaactt tcttctttca accagtccat ataactgtca tctattatct
     4621 cgaaaaaata atttatagca ggatttctat cccaatcatt tttttcttta ttttcaaata
     4681 aatattctga aaattttact ttataagatt catctgttat ttgaaaatga attatattgt
     4741 caataaatat ttcaatattt aattcataat ttcggtcgga tgccaaaatg ttcacatctt
     4801 gttcggtaac acaaattttg tctacgtaaa atctgcgact attgttagaa aattgtaatg
     4861 gtttaatctt cacaatcgtt ttccatatct aatttttgtt ttcttattgc cctgccagat
     4921 aaagcaggtc tgcctccgta ctgtctatcc tcacatttac tttcctgcca tcggctttta
     4981 tctccccggt tttaccgttt ttgccaactt tgcggctttg gcggcggcaa tgttgaaggc
     5041 ggcttcgacg gtttcggcgg cattggggtt gacccgtact catccgcaaa cggcaacacg
     5101 acggacggtg tttgcttttc agccgtctgc cgttttgacc aaggtgctgc cgtggaagga
     5161 gcaagtgtag gatttttgaa gaactcagtt ctcaatatcc tatttcaaac gaaggaaatg
     5221 ccgtctgaaa attaaacacg gtttcagacg gcattttgat tgttgggttt tcaacccgac
     5281 ctacggttcc taatgatttt cagataaaaa taaagaaata tcttctaacg catcttcagc
     5341 cctaccttga agctctggat catcttgctt tgaatgtagt aaagaaataa ctttatcgta
     5401 atcaccgata tttgaatgta gcctggcgag atgtccgaaa caggttagcg ataatccggc
     5461 aatatccaag tctttatttt tcgataattc catcaatttc tcagaaatcc acggtacttc
     5521 atatgctttg gaattaaata ccatatcaag aatgccatct ataacacttt gtttatcatc
     5581 agaagaaatc aacttttctg ttttactttt atcagtcatt ttttaatttt tccttttgaa
     5641 ttaactaatc caatcctgct ttttttaaag catttttctg atcggtatgc aaatcttgcc
     5701 aacttctgac atgcccgtga tattccgcaa aaccgttatt aaaagtttga gttttgtcta
     5761 atacaacgat ttcattattg gctttatcaa ccccaactct tcgaggagaa gttgatttaa
     5821 cttgaacgga attatcaaga gctgcttgtc cgtttgttgg tgctctactt ttcacagaat
     5881 tatcattttt gccatgataa gaagcgtctt tatatcgttg gttgccttta tcatacggac
     5941 aatcattatg aacccaaacc ccttccgttt ccgcctgact gcccttgacg aagtaggtat
     6001 gccaatcggc gacggtcaga ttgtaggctt tgagcggctg ctgtttgaag gtaatgtttt
     6061 gaaccgtctg ttttgcgccg ctttcggaaa gcagggtgtc gccttttttc agacgacctg
     6121 cctgtatcca ttttccttga ctgtaaaacg ggtggatttt attggaaatc agggtttggt
     6181 tgttgccgat gccgtctgaa atttcaatgt aaacggtttc ttgatacgga ttgccgtatc
     6241 gggcggtaac gggtttgtat cccgtttttc cgcttgcctc gtccttggcg aagacgcggt
     6301 cgccggttcg gatacgggca atggctttgt agccgtctgc cgttttgacc aaggtgctgc
     6361 cgtggaagga gcaggtgtag gatttttgaa gaacttagtt cccaatatcc tgtttaaaat
     6421 aaggggaagg tcgcctgaaa attaaacacg gtttcagacg gcactttgat taaatttaag
     6481 aaaatcctta ccaagtaaac ggtttccgat tcatatccga aaggttgcca acttcattat
     6541 ccagcaagaa ctgctcaaaa gcattccaac cctttttttc caccaattct gcttcctgtt
     6601 tatacaacgg gacaagcaaa gggaaaacga tattgtagtg ttcgccataa cagacctgaa
     6661 aatcatcgtc gaaataaaat ggggcggaaa catacagtgc atccattttg ctaccttcga
     6721 taattaccct gggtaattga ataatttcac ctcttaaaat aggttgtcta ttgtcaatca
     6781 aatattcagc cactcctgat agaaaagctg caatattttc tttattccat tcttcagaac
     6841 aggtaaaaat taattcaaac ctactcttat agttcaagtc atatcgattc aagcctaaag
     6901 tagaaaaagt agttacccct ttatatggtt gtgatgggaa gacggaaacg ctgagatgat
     6961 aacgtcgatc caagcatttt ataccgcttt cgatttcacc caaataatgt tctaaatgtt
     7021 caactataat cattttttct taaagatttc atccctatcc cggcaagacg taggctgttt
     7081 tgggctccaa taccgacttt tttattgacg gaaatgccgt ctgaaaaata aagcggcatc
     7141 ttgattttgc cgtctaagga ctttgcatcc attcctcttt gctgattcca aattcaaatt
     7201 cagcctgttc aaatgccgaa tctaatgtgt catgataagt atccgtttgt tcctgaccgg
     7261 tctcatccaa ataaaacaaa taaaatcccg ggtcattttc atattgaaca atacttaaag
     7321 caacaggttt gggaagtatc cccatcgggg tacgatgaat cgttctaata ggaaaatctt
     7381 tattttcatc taagatgact ttgaataatt cttttacact catcattctt caatcccgaa
     7441 taattgttac cgcactattt tctattacac gtccattggc tttgtagccg tctgccgttt
     7501 tgaccaaggt gctgccgtgg aaggagcagg tgtaggattt ttaaagaact tagatctcaa
     7561 tatcctgttt taaatgaagg aaaagccatc tgaaaattaa acatggtttc agacgacctt
     7621 ttgattgttg tgtttttcaa cccaacctac acttcttgtt agttcccata cgaaccaaaa
     7681 ccttcataat tacaatcatt tttttgtgca atatcaaata acgtagtttc aatagctaaa
     7741 atgttttcaa gagaagggat aatattgacg gaacaatagc aagtccacat ttctccgaat
     7801 tcattttgct caatactgat ttgaaaatct tgccgataag aaatgatttg actttctatt
     7861 gattgcagat tcttttgaga accctctata aaaaactcta ttatcatcga ttttgtcaaa
     7921 tcaaacccta gttcttgata ttgtttcagt acatctgaag tagatttttc gtcaacaatc
     7981 atttttaatc tccatcttgt tttcgatatt ttttccccgg gctttcaatt cgattgtaat
     8041 tattggtact tttggaacca ccgtgagcgt caattcttct agattcatct ttgaatgcct
     8101 ctcgattatt tttggcagga gtaaaatctg tagctatatg agggtcatta ttaaccctag
     8161 cttctcttgc tccagagatt tgggaacgtt gtcttgtgcc ctttccagaa taagttcgac
     8221 cactttcatg agtattagta taagaaccaa cttctgtcct atatttttct ccatacggac
     8281 actcattatg aacccaaacc ccttccgttt ccgccttatc acccttgacg aagtaagtat
     8341 gccagtcggc aacggtcagg ttgtaggctt tgagcggctg ctgtttgagg gtaatgtttt
     8401 gaaccgtctg ttttgcaccg ctttcggaaa gcagggtgtc gccttttttc agacgacctg
     8461 cctgtatcca ttttccttga ctgtaaaacg ggtggatttt attggaaatc agggtttggt
     8521 tgttgccgat gccgtctgaa atttcaatgt aaacggtttc ccgatacgga ttgccgtatc
     8581 gggcggtaac gggtttgtat cccgtttttc cgcttgcctc gtccttggcg aagacgcggt
     8641 cgccggcttg aatatgggca atggctttgt agccgtctgc cgttttgacc aaggtgctgc
     8701 cgtggaagga gcaggtgtag gatttttgaa gaactcagtt ctcaatatcc tatttcaaat
     8761 gaaggaaatg ccgtctgaaa attaaacacg gtttcagaag acattgtgat tgttggtttt
     8821 ttaacctacg cttgcgaaat taattccttt ctcctgaata ctgcaccatt tatttggttt
     8881 aactccatag aagaatgatt agaataaata cattcaaagt cagcttcatc aaaatattct
     8941 ccacacatag accataactc tgtaatcaaa tattccatat cttcatggtc ttctgtgatg
     9001 gcttctcttg ataaataatt tttgattttt aaaaaccttc ttctttcctc gtaatctacg
     9061 gtaattaacc tgatatctga gtgtatcatg cccagcattg ctatattcat accgactcgc
     9121 agccagcttg gtaatatatt cataactatt gcaccttttt aatggtttta gggttagccg
     9181 gaacaatgtg aactccattt ttacctgaat gaatcgtacc aaaattggta cttaatcctg
     9241 atttcccatc gctgcctata ggatacccaa aatcaacaac aggatttcct cttgctcctt
     9301 tagaaataac tggataagca cctgaatgta ttccgttcaa caattcttga ggattaatgt
     9361 tttgatttaa agtactctta ccttcaatgt agtttctatg tcctgaaata tgtttccctt
     9421 gagctccatc atgaatttta gtcccaatag attttgcttt aaaatcaaca ttgcgggttt
     9481 gaaacatttc tccatctaca gactgagata atcttgaagc agtgttatgc ttgtaggagt
     9541 ctgagaaatc cccactaacc gcagccttcc ccggttttgc cgcctttgtc aggtttttga
     9601 ctttggcaaa cggcaggacg ttgaccaggg cttcgacggt ttcggcggca ttggggtttt
     9661 cctgcatcca ctggtcaa
//
LOCUS       DAWEWP010000066         9652 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWEWP010000066 DAWEWP010000000
VERSION     DAWEWP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.066"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..547)
                     /locus_tag="WGN53_001170"
     CDS             complement(<1..547)
                     /locus_tag="WGN53_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0140165.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIR"
     gene            complement(579..962)
                     /locus_tag="WGN53_001171"
     CDS             complement(579..962)
                     /locus_tag="WGN53_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0140166.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            1331..2035
                     /locus_tag="WGN53_001172"
     CDS             1331..2035
                     /locus_tag="WGN53_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ0140167.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(2099..2665)
                     /locus_tag="WGN53_001173"
     CDS             complement(2099..2665)
                     /locus_tag="WGN53_001173"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ0140168.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2731..3147)
                     /locus_tag="WGN53_001174"
     CDS             complement(2731..3147)
                     /locus_tag="WGN53_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140169.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(3199..4098)
                     /locus_tag="WGN53_001175"
     CDS             complement(3199..4098)
                     /locus_tag="WGN53_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ0140170.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4129..5586)
                     /gene="der"
                     /locus_tag="WGN53_001176"
     CDS             complement(4129..5586)
                     /gene="der"
                     /locus_tag="WGN53_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ0140171.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5740..6369)
                     /locus_tag="WGN53_001177"
     CDS             complement(5740..6369)
                     /locus_tag="WGN53_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ0140172.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RATSQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISTAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6370..7665)
                     /gene="hisS"
                     /locus_tag="WGN53_001178"
     CDS             complement(6370..7665)
                     /gene="hisS"
                     /locus_tag="WGN53_001178"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ0140173.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            complement(7791..8333)
                     /locus_tag="WGN53_001179"
     CDS             complement(7791..8333)
                     /locus_tag="WGN53_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0140174.1"
                     /translation="MQSWKARRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVRQIRQARAE"
     gene            complement(9450..9650)
                     /locus_tag="WGN53_001180"
     CDS             complement(9450..9650)
                     /locus_tag="WGN53_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140175.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         2264 a         2322 c         2457 g         2609 t
ORIGIN      
        1 cccgtatttt cttaccgtat tctgcgattg cctgtcggaa aatgccgatc aacctgccta
       61 taacggcatt ttcgccaaat tcgttcagac agttttccct aagtcgggca ggttcaaaat
      121 cagagtggtg ttcatacatt ttgatgagtg cgtcggcaag ggcatcgtcg tcgtcaacag
      181 gaaccaaata tccgttgccg tctgaaacaa tagattccgc accgccgcag cgtgttgcaa
      241 tgacgggcaa tccttgggac agtgcttcga tatagactac gccgaaggtt tcggtgcggc
      301 tggcaaggac gaatgcgtcg ctgttcctca tcaaatccaa gactgcttcg ggctgcaatg
      361 cgcccaaaaa tgtaacggca tgggtaatgc ccaagtctgc cgcctgctgt ttcagccgct
      421 gttcttcctg tccgctgccg ccgatgttca ggcgcagttg cgggcattgt gccaacgccc
      481 gggcaaaggc agtgagtagg acatcgtgtc ctttgagacg gcgaaggtgc gagacggtgc
      541 agaacacgaa atgcggtttg ttgttttttt ctctttgttc aaacggtcgg ttgaatattc
      601 cgcccagtat gttgggggag gtactgccat tcgcagccgt atttgtgttg caggacgtgt
      661 gcgaaatggc ggctgacagc gagcagtgcg gaggcgtgcg ccgctgcttt ttccatcggc
      721 tgccattggt ggggacggat cagaccgcgc gtgatggtgc tgctgtgttc cgtgacgaca
      781 taggggatgc cgtatttttg ggaaattttt tgggcgagta tgccggcata gttcatgcgg
      841 tgggcgtgaa tcaggtcggg cagcccgttt tcgcggatgt agtgtttgaa agctttcaaa
      901 cccgcacaca cccagcggat gcagtcgatg tcgatgaacg gaaagcgggg gaagaaatat
      961 atgccgtgcc atgcatagat gtccaaaccg ctttgccgat attttgcaaa accgtaaggc
     1021 cggtcaggat gcttgctgtt tctttccgca agtagcggaa catcggtgca agcacggcgg
     1081 ttttgatgcc tttcctctgc aatgccagtg cctgattttg aaaaaaatcc cgtccacatc
     1141 ctgttcggat tgcggatacc atgaggggat gacgaggacg tgcaagggtt cgggcatagt
     1201 gggattccgt atcggaaagg cggttattat aagacagacg cagaccgaat atttaaattg
     1261 ttgccttacg ctaatgcaat ttagcgcgcc gatatgctag attggcggtt ttataagtaa
     1321 ggatacggat atgttgcgtt ctattttggc ggcttccctg ctggcggtat cttttccgac
     1381 ggcggctgaa gcattgaatt acaatattgt cgaattttct gaatcggcgg gtgtcgaggc
     1441 ggttcaggat acaatgtccg cacgtttcca agtgacggcg gaaggacggg acaaaaatgc
     1501 cgtcaatgcc gagtttgtta aaaaattcaa caatttcacc agaaaatcaa aaaatggtag
     1561 ctttaaaacc gaattggtat cgcgcagtgc gatgccgcgc tatcaatata ccaacggcag
     1621 acgcattcaa acaggttggg aggagcgtgc ggaatttaag gtcgagggta gggattttga
     1681 tgcgttgaac cgttttattg ccgatgttca ggcagatgcc gcgttggaat atacggattt
     1741 ccatgtgtcg cgcgaacgcc gcaacgaggt catcgatcag gtcagcaagg atgccgtttt
     1801 gcgtttcaag gcgcgtgccg aaaagttggc gggcgttttg ggtgcgtccg gttataaaat
     1861 cgtcaaatta aatttgggac acatcggcag ccatatcgcg ggagggggag ctgctcaggc
     1921 aaaaatgctt cgtgccatgc cgatggcggc aagcgtcaat atggagggtg cggattccgc
     1981 cgcgcctggt gtggaggaaa tcagcatcag cgtcaatggg acggttcagt tctgatttga
     2041 ggtgaacggc aaatgccgtc tgaaacccga cgataagggt tcagacggca tttatatttc
     2101 aggctttggg cagggtaacg ccggtttgcc ccatatattt gccgttgcgg tctttgtatg
     2161 aggtttcgca gatttcgtcg ctctcgaaga agaggacttg cgccacacct tcgcctgcgt
     2221 agattttggc gggcaggggg gtggtgttgg aaaattcgag ggtaacgtag ccttcccatt
     2281 ccggttcgaa cggggtaacg ttgacgataa tgccgcagcg ggcgtaggtg gatttgccca
     2341 agcagacggt caggacgttg cgcgggatgc ggaaatattc gaccgtgcgc gccagtgcga
     2401 aggaattggg cgggatgatg cagcagtcgt cttcaacggt aacgaagttt ttcgggtcga
     2461 agtttttggg atcgacgatg gtgctgttga tgttggtgaa gattttaaat tcgtttgcgc
     2521 agcggatgtc gtagccgtag ctggacgtac cgtaggagat gatgcgtttg ccgtcggctt
     2581 ctttgatttg gttcggctcg aaagggtcga tcatgccgaa ttcttcgctc attcggcgta
     2641 tccatttgtc ggacttgatg ctcataatgt tttccttgtt tcttgcagtg ttcggacaaa
     2701 gcattggggg atgccgtctg aaaacggggc ttatttgttt ttgggcagct tcacttcttt
     2761 aatcatgccg ttttcgcatt tcataatgaa gagggtttcg ccgttttgcg agacggtaaa
     2821 tctgccgtgt gccaagcctt ttttgaatgt gcccgagagt accatgttgc ggaatttggt
     2881 actgtcggaa ttgaacggtt cgataaatat ttcgcggttg gcggcaacgg tataaacgcc
     2941 ttgtccgtcg aatttgccat ttttaaacga accggtatag ttgcgcccgt cttggcagcg
     3001 ccatgtgcct ttgccggcgg gtttaccgtc tttgccgaca tcgccttcgt aggtgcagcc
     3061 gggttcttga taggaagtca gaacggcgga cgcggcgggg agggcgaaca tcatggcggg
     3121 cagtaggaat gcgagatgtt taagcataag ggttattcca ttggattttg gctgacggta
     3181 taaaagccgt ctgaaaaatc aatcttgcca gccgcccaaa taggaaacca gctcttccaa
     3241 catgatgctg acggattccg ccatcaggat ttgcgaggcg aaggcgagac cggcggcatc
     3301 gtcgccgttg ctttcggctt cttcctgcaa tacgtcgagg tattggatgc gcttgagtgt
     3361 gaagtcttga gtgaggataa aggcgatttg ttcgcgccaa accaaaccga gctgggttac
     3421 ggttttaccg tttttgacgt gttgaaccac ttcgtcggcg gttaaatctt gtttggatac
     3481 tttgacgacg ggaacaatat cgcccgtgcc tttgagttcg caatcgctgt ccaattcaaa
     3541 accgccttcg caatgccctt gcaacagcca gccggtcatc aaggaagagg gcgattgctt
     3601 ggtattcggc agcgaggctt ccaaaccgcc caaagcttcg cgcagcttgg tcaggatgtt
     3661 ttctgctttg gcggaagccg cgttattgac gagcaggtag ccgtggcggg tgttaaacac
     3721 cgcttctgta cggctgctgc gggtaaacgc tcggggcagc aggtcgtctg taatttgctc
     3781 tttaagctct tgtttttctt tacggccgac attgcgggct tcattgtttt ggatttccgc
     3841 taccttctct tccaaaatat cgcggatgac gccggcaggc aggacttttt cttctttttt
     3901 cagggcgacg cgcaaggtaa agtcggcagg gaaaacgagt tcaggggaga atgaaaccgg
     3961 tgcggtaaag ccttcgctga accagtctaa gccttggcaa tgggtaaatt cagcttcagc
     4021 aagtttgtcg gcaagtacgt ctgcctcagg cagcttttct ttgttgagcg gataaaaact
     4081 aatctgcttg aaccacataa tgtttcctat tgtttgaaat gtcgggaatt atttgctgaa
     4141 ttgttttttc acactgactt tggttttctt cttgaagcgg tttttctctt ctttgcggcc
     4201 ttcgcgtttc tcaatacggt tgctcaggct gacgcggcgc agcggttttt tcttcggttt
     4261 gtcttccgca ttttcatacg ggttttccga aacattgtat tgaatccgca acggcgtgcc
     4321 ttgcaggttg aaggctttgc ggaacgtttg ggtcagatag cgcgtatagc tgtcggaaat
     4381 cgcgtgcagc gaattgccgt gtaccacaat tacgggaggg ttcatgccgc cttggtgggc
     4441 ataacgcatt ttcggacgca ccaagccggc acgcggcggt tgctgacgct cgatcgcgct
     4501 ttgtaatacg cgcgtgattt tcggcgtcgg catcttaatc atcgccgcgt tgtaggcagc
     4561 ctgaatgctg tcaaacaaac cgtctatacc gcgctctttc aatgcggaaa taaagtggaa
     4621 cttggcaaaa tcgaggaaat acagtttgcg gttgatatcg cgtttcactt gctcgcgacg
     4681 ctcttcgctg atgccgtccc atttgttgac ggcaaccacc aaggcgcggc ctgcttccaa
     4741 agcaaaaccg gcaatcgtcg catcttggtc ggcgatgtcc tgctgcgcgt ccaataccaa
     4801 aacagcgacg tttgccgctt caaccgcctg catcgctttg ataacggaga atttttctac
     4861 tgcttcatcc actttgccgc gacggcgcac gcctgcggta tcgatgatgg taaacggttt
     4921 gccttcgcgt tcgaaatcga tgtggatgct gtcgcgtgtc gtgcctgcca tatcgaaagc
     4981 aatcacgcgc tcttcgccga ggatggcgtt aaccagtgtg gatttgccga cgttcggacg
     5041 gccgatgacg gcaaagacgg gatgttttgc atcggcttct tcggcttccg gctctgggaa
     5101 ggtttccaaa atatcttcaa tcagataata cacgccatcg ccgtgcgcac ctgaaataac
     5161 ataagggtcg cccaaagcaa gttcgtagaa ctcggcggca agcacggccc tgttgccgcc
     5221 ttcgcctttg tttaccgcca gataaacagg gcgcgggctt tggcgcaggc ggtcggcaat
     5281 gattttgtct tgcggggtca ggccggtacg gccgtccacc aaaaacacaa ccgcatcagc
     5341 ttcatcgaca gcctgcaagg tttgttttgc catttcgtgc aaaatgccgc tgtccacaac
     5401 cggctcgaaa cctccggtat cgatgaccaa ataaggtttg ctgccgactt tgccgtgtcc
     5461 gtaatggcgg tcgcgggtca ggccgggcag gtcgtgtacg agtgcgtctt tggtgcgcgt
     5521 caaacggttg aataaagtag atttgccgac gttagggcgg ccgacaagcg cgatggtggg
     5581 tttcattatt gggtctttct gtgtcaagtg ccgttcggga gaactgaaca cgagcaggtg
     5641 tccgttggac acggtaaata ggttcttcac ggcaagaggc cataaagagg gagggtaaaa
     5701 tgccgtctga taaataatgg acagcatttc agacggcatt tatttcagcg aatcaagttt
     5761 catttgaacc aattcgcgac cgacagaatc ttgaggcatt ttttctaaag cctgtccgta
     5821 gttttttaag gcttcctggc tttttccctg tgcggcatag acatcgcctt tggtttccat
     5881 cagcaggggg gcgaagtccg cttcaaccgg cgtatcgagc gcggcaagcg cggcatcgta
     5941 ttttttttgt tgcaacaaca caacgcccag acgctgcgcc gccaacgctt gaatcaggct
     6001 gtctttttgg ttggacaaca cccatttcaa atggccttcg gcaacatcgt aacgctgtgc
     6061 gtcaaattcg gttgccgccg ccatcagcgt ggcttgggcg gtggaaatgg aatgcgggta
     6121 gctttgctgg agtttggtca attcggcatt gatttcgctt tgcggggctt tgttttgcgc
     6181 cttttccacg atgtttgcca gcaccgccgc cgcttcctga ttttgggaag tcgcacggtt
     6241 ttggtaaacc gtgtatccca agtagccgag cgccgccaaa atcagcaggg caaacagcca
     6301 tttgcccgtg gttttccaaa aatatttaaa gttgtccaac tcttgttgtt cttccaaatg
     6361 ggctgccatt tatgcgttct tccattgttg taaagtgttt gtcaaatcag cggcggcgac
     6421 agtttgctga ccgtgtgcgc cgttcatgtc tttgagcgta accgtaccgt tcgccagttc
     6481 gtcttgcgcc acaatcaggg caaagcgcgc gccgctgttg tcggcttttt tcatttgtgc
     6541 tttcaggctt tgatagccgg aatgctgcat tacattgaaa ccttgcgcgc gcaaggcttg
     6601 cgcgtatttc atcacctgca agtccgccct ttcgccttgg tgcattgcat agacatcagg
     6661 cgcagcgttc acttccagcg agccgtattc gctcaccaaa agcagcagcc gttcgatgcc
     6721 catcgcaaag ccgatggacg gcgcaggttt gccgccaagt tcctcaatca aaccatcgta
     6781 acggccgccg ccgcacacag ttgcctgcgc gccgagtttg tcggtcgtcc actcaaaaac
     6841 cgtctgattg taataatcca aaccgcgaac caagcgcgga ttttcaatat attggatacc
     6901 caaaccgtcc aacatcgcct tgaagcgtgc atagtggttt tgcgaagcct cgcccaaata
     6961 atccaccaaa cgcggcgcgg cgttgcagat ttcctgcaaa tcagggtttt tcgtatctaa
     7021 aacacgcaaa ggattggttt tcagacggcg tttgctgtct tcgtccaatt tatcttcata
     7081 gcgtgtcaaa tattcaacca acgccgcacg gtgtgccgct cgctcctcac ggttgcctaa
     7141 gctgttgatt tccaaagtca ggtattcgcg gatacccaat ttttcccaca agtcggcaga
     7201 catcgcgatg atttccgcat cgatatccgg cccttcaaaa cccaaagcct cgataccgac
     7261 ctgatggaat tgacgataac ggcctttttg cggacgctcg cggcggaaca tcggccccat
     7321 ataccacagc ttttgcgggc tgttgtacag aaggttgtgt tcgaccaccg cacgcagaca
     7381 agaagccgta ccttccggac gcaggctcaa actcaaagaa tcgtttgaat cggaaaaggt
     7441 gtacatttcc ttgccgacca catcggtttc ttcgccgatg gagcggacaa acaaaccggt
     7501 ttgctcgaca atcggcgtac ggatttgctg atagccgtaa gcgcgcgtcc agcggtttac
     7561 cgtatcctca aacgcctgcc aaaacgcagc cgtcagtttg aaatcttttt gctcgacagg
     7621 cagaaggtcg ttcatgcctt tgacggattg gattttttgt gccatttcaa gtaagaatgc
     7681 ttaaatcaaa ttgcgggcga ttatagcgga tttttaaggg tttgtgaggt tgggggtggt
     7741 ttgcggacgg gtaaaatgcc gtctgaaaca gttttcagac ggcatttgac ttactctgca
     7801 cgtgcttgcc tgatttgtcg gactgcaaac tctgtctgcc gcttgggatg atgtgtgaaa
     7861 aacaatttat ttgaaattgt ctcggctttt ttcggcacga ccgccaaaat cttacctgcc
     7921 aaatttccct cacgggtttg ccaagcatcc aaaaactgcg ccctgctcat cgaaacatga
     7981 cccagcgacg ggtcggcaag caaaaccgta ttgccgtcta taccgcgcaa taccgagaaa
     8041 tgatcatcct tacggtattt cagatacacg atgacgggga ttttcaactg cgcgagctgc
     8101 tcgaaagaca gggcatagcc tttcgcttca aaacccaaat ctggcataat gcgccgcata
     8161 tcctcaaacg acgcgcgcat ctgctcctta tccagctttt tcaacacttc ttcttccgtc
     8221 agcgtttgcc cgtaaaaatt gttcaaaagc gtcgccaccg aagccgcccc gcaggaaaaa
     8281 tccaaatcct gctttacaat attgaaatcc cgccgcgctt tccaactctg cactttgatt
     8341 tttccgtaaa caacaggatt atcgttaaac atcggtgcag cattcagacg ataagataaa
     8401 gaaacgacaa cacacgccaa cagaaaaaca tatttgaact tcatcatatt gtccacataa
     8461 agggcagcct gaaaatcttt caggctgccc ttgtcaaatt attcctagct ttcggctttt
     8521 ttggcaaacc aaacaatccg attacccgca taatactttc catttattga aatccgacaa
     8581 gccgcgccca aaaatgccat gcactgtcga tttccgcagc aatctttgta ccgttttctt
     8641 caaattccaa atattcaccc aataataaac ttgaaacaga acgcgtgggc agcaagtgcc
     8701 caccctacgc ttattcaaat aatttgatta aataaagaat aaagaaaatg catcaggaac
     8761 aaaattataa tctgccacct gactcacacc gcttttaaag taaggggcat caaaatcaaa
     8821 accgcaaaaa aaataatttt tgcattgatt tttaatagat ttaaaattcg aatatagtgt
     8881 ttctccagat ttaaaaagtt tatctttatc tatatatttg atgctttccc tatccaaaat
     8941 aaatattcaa acattaaaaa atcattacat gaccaagcca aatcataaat ttgattcaaa
     9001 tgggtatcat aaagataaaa ataataaggt ttgggaacag gtaaaatatt taatggaaaa
     9061 ggaggactaa ttttcttaat atctgatgac tgatatccat atctttctat attcttaaaa
     9121 atttcatctt tcttaagata acaagacatt ctgaagataa caagacattc tgacaatcac
     9181 aacctgttca tcggatatat tatctatttg catggcgcgt ataacacgcc atgcctgatt
     9241 aaaattagtc tcccttacct tagaatctat tatcttttcc aaagatgaaa atgcccctta
     9301 tcttgaactc tccagttaga acctggaagt ttcgtacctc ttaaatgtgt attaatattt
     9361 cttatagttt cattaaaatg ccacgcgctg ccgatttcaa cggtaatttt cgtaccgctt
     9421 tcttcaaatt ccaggtattc ccccattagc taacgcaaag aagcagacgc catttcggct
     9481 tcgttatgat aaacccgcct tccgttgata tagacttccg cccctgtccg ctccaaattc
     9541 caaaaattcc gtactgaaat ttccatatcc cgatattgtg cagaccatgt tttttcgaag
     9601 gttttcataa aatttcctat acctgtccaa tcggcacata tcaattgcat ta
//
LOCUS       DAWEWP010000067         9652 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWEWP010000067 DAWEWP010000000
VERSION     DAWEWP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.067"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(45..3077)
                     /locus_tag="WGN53_001181"
     CDS             complement(45..3077)
                     /locus_tag="WGN53_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ0140176.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNGAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPSGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSSGQYLSETEAFGHDSRAVCPFEDVPSERPSCRVSDTEADE
                     GAFQSEETDAVYEHLPTTDLLLPPLFNPGATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3242..3922
                     /locus_tag="WGN53_001182"
     CDS             3242..3922
                     /locus_tag="WGN53_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ0140177.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLACAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3951..4310
                     /locus_tag="WGN53_001183"
     CDS             3951..4310
                     /locus_tag="WGN53_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ0140178.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
     gene            complement(4443..4871)
                     /locus_tag="WGN53_001184"
     CDS             complement(4443..4871)
                     /locus_tag="WGN53_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140179.1"
                     /translation="MISIFKAYYDVYEFNGELLKRCIFLDEENEENNEIIKDRIYGYL
                     IYVLTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYNNEFDEE
                     DGWPDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            complement(5354..5890)
                     /locus_tag="WGN53_001185"
     CDS             complement(5354..5890)
                     /locus_tag="WGN53_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ0140180.1"
                     /translation="MDLREVKLGGETIYEGGFVSISKDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5924..6280)
                     /gene="folB"
                     /locus_tag="WGN53_001186"
     CDS             complement(5924..6280)
                     /gene="folB"
                     /locus_tag="WGN53_001186"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ0140181.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            6336..6938
                     /gene="plsY"
                     /locus_tag="WGN53_001187"
     CDS             6336..6938
                     /gene="plsY"
                     /locus_tag="WGN53_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ0140182.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAVLTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHLSWIWATLLIALLVLFRHKSNISNLLKGKEGKIGEKR"
     gene            7026..7871
                     /locus_tag="WGN53_001188"
     CDS             7026..7871
                     /locus_tag="WGN53_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ0140183.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPKLYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKELISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            8117..9091
                     /locus_tag="WGN53_001189"
     CDS             8117..9091
                     /locus_tag="WGN53_001189"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ0140184.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(9250..9468)
                     /locus_tag="WGN53_001190"
     CDS             complement(9250..9468)
                     /locus_tag="WGN53_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ0140185.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         2272 a         2800 c         2190 g         2390 t
ORIGIN      
        1 gccgtctgaa acagtcttca gacggcattt ccatttgcaa aaaatcaagc attgtccaag
       61 gggacgagaa tcgtacggtt gccgttgtgt tccggtgcgg acacaatgcc ttccgcctcc
      121 atctggtcaa tcagacgcgc ggcgcggttg tagccgatac gcaaggcgcg ctgtacgccc
      181 gaaatgctgg ctttgcgcgt tttcaggaca acggatacgg cctcgtcgta catcggatcg
      241 gtttcgtcgt cgccgctgcg cccgatgccg ggcagctctt cgctgccgcc gccgctcaaa
      301 atatcgtcaa catagtccgg ttcgccaaac tgtttcaaat attcgaccac gcggtgcacc
      361 tcttcatccg aggcaaatgc gccgtgaacg cgctgcggat aggcagtacc cggcggcagg
      421 aacagcatat cgccctgacc gagcaggttt tccgcgccca tttggtcgag aatcgtgcgg
      481 ctgtcgattt tgctggacac ttggaacgcg atacgcgtcg ggatgttcgc cttaatcaga
      541 cccgtgatga catcgacgct ggggcgttgt gtggcaagaa tcaaatggat gcctgccgcg
      601 cgggcttttt gggcgaggcg ggcaatcagt tcctcgattt tcttgcctgc cgtcatcatc
      661 aaatcggcaa actcatcgac cacgaccacg ataaacggca gtttttccaa aggttcggga
      721 tcgtcgggcg tgaggctgaa cgggttgccg attttctccc cccttgctgc ggcttcggcg
      781 attttttgat tgaaacccgc aagattgcgc acgcccataa agctcatcag gcggtagcgt
      841 ttttccattt cgttaacaca ccagttcagc gcgtttgccg ccagcttcat atcggtaacg
      901 acaggggcga gcaggtgcgg gatgccttcg taaatgctca attccagcat tttcggatcg
      961 atcataatca tacgcacgtc ttccggcgcg gctttgaaaa gcatagacag aatcatcgcg
     1021 ttgacaccca ccgatttgcc cgaaccggtc gtgccggcaa ccaacaaatg cggcgctttt
     1081 cccaagtcgg ttacgacggg ctgtccggtg atgtcctgac cgagcgcgag cgtcagcttg
     1141 gatttggatt cggcaaactc gggtgaattg aagatttcgc tcaggcgtat catttggcgt
     1201 ttcgggttcg gaagttccaa acccatacag gttttgccgg ggatggtttc gacaacgcgg
     1261 atggaagcca cgccgagcga acgcgccaag tctttttcca gattcagaac ggaattgccg
     1321 cgcacgccga catcgggttc gatttcataa cgcgtaatca cggggccgga ataagaatcg
     1381 acaaccttga ccttgacttt gaactccgcc aatttttctt cgatggtgat gctgttttcc
     1441 aacagttctt cttcggtttg cgtcgccccg ggattgaaca gcggcggcag aagcaggtcg
     1501 gttgtcggca ggtgttcgta taccgcatcg gtttcttccg attggaacgc cccttcatcc
     1561 gcttccgtat ccgatacccg gcaggacggg cgttcagacg gcacatcttc aaacggacaa
     1621 accgcccgac tgtcatgccc gaacgcttcg gtttccgaca gatattgccc gcttgaccgc
     1681 tctgccgcac cctcactgcc gtcatccgca atagcggcgg tttcctcctg ccaacctcca
     1741 ttcaaaacat catcggcaag atggtcggtt tcggcaatgc ggctgcgttg cgcctgctcg
     1801 aatcctgacg gcggttcata ggtacggttg tagatttccg atacgggagg cggcggcgga
     1861 atatcgattg cgggcatggg aactttcggc acttcgggtg gttcgaccac ccaagcatcg
     1921 ggcgcggcag gttcttcaat atggatatcg gcagtttcat cataaaccgg accgccgtat
     1981 cccacagacg aaacggtttc cgtgaaaaca ttttcggttt cagacggcat ttcgacatcc
     2041 cgtttcccga caaccgtccg actttccgga atcaggcgcg aaatccgcgc ctccgcagaa
     2101 accgttgcct tgttcggttc tttcaaattg acggaaacgc ggcggcgggc atcgcggacg
     2161 gctgtgccgt ctgaaacgga ctgtccttgg gaaacatccg gggattttgc ctccgcctgc
     2221 cctgcatgac ggcggaacgg cgcattccgt gcattttcgg ctttgactgc agaaaaggca
     2281 gacggatgct gcttctcaaa atacggctca aatccgtaat ccgcagactc ggaaaacgct
     2341 tctttgtccg catcgaacat acgggaatac gtgcgttgaa gcacagggtc gtccaaaccg
     2401 ataatctgca gcccttccat cggggtatcg gacacgcgga ctttggggac cgctgtttcg
     2461 tttttctcaa atgcatcgat atagcgtttt ttcgtttccc ttaaagccgc gccgtttaag
     2521 gcacgcgttg cttcttccaa agtgatttcc ttaaaaaccg gacggacggg cgaaatttcg
     2581 ctttcagacg gcatcaaccc ttcagcaata ctccggtcga atgggatgcg gcggttgtcg
     2641 attacggcag ttgcaatgtc ttccgtatcg gcagcctctt cctccgcagc ttctgctgct
     2701 tccgtttccg cttcttccgt cccgtttcca ctgtctgaat atccgtcgga tgcgttcgga
     2761 acatccgcac ggtttgcaga agcaggcgga acaggcctat ccggttcgga ttcgtgcgcc
     2821 agatagtttc ggcagaaacg gacgacaccg acgaacagcc aatacactgc cgttttgacg
     2881 gcatggaaaa gcatcagggc aagttcggga aaatcgggca taccgtcttt gatttcaggc
     2941 agctccgcct gtttttcccc tttcttttcc tgccacgcag aaacctcgcg catccactcg
     3001 cgttcggatt gtgcgcggac aaaaaacagg ccggcaagcg caagcaacaa aataacgatc
     3061 aaaactatcc aaaacatatg ccgtttcccg caagacaaac gattaaacgg gtttatttta
     3121 acgtgtttcc atcccaaaaa aaacaaccag ccggcaattc tgccgaaacc gattcggctt
     3181 tcggtttgcc cccgccgaca gtataatagc cgtcccttcc cttaatcttt gcggcattgc
     3241 catgattttc caaacagtct ggttttcaga tgtggtactg tcggtttcgt ggattgtcct
     3301 tatcctgata ctggcagctt ccgcgccgtc cgcattccgc tcgcttgccc gctaccgttc
     3361 cgcccttcca ctgtgtaccg taattttttc cgcagcatgg tgtctgaacg cctctgccgg
     3421 cggcggacag cttgcccaga tgaactacca tctgctcgcc gtcaatctgg ttgcactgat
     3481 ggtgggcact tccgccgcct tttggcttgc cgcgctgctg atgctgccct actgcctgct
     3541 gtttgccggt tctgccggcg catatccgcc gaacgctttg gtgctgattc tgcccgcgct
     3601 tgccgtcaac cgcctgtcgc gtatgctggt caaccgcctg ccgcccaata ttttcatctt
     3661 tattttcgtc aacggctttc tcgcttccgc cgccggcatt ctgttgaccg ggctggtgct
     3721 gaccggtatt ttggatgctg caaacgcttt tccgtccgaa atattgtgga cgaccgccct
     3781 gcccgtcttt attctgctgg cgtgtgcgga agcttttctc agcggcattt caactgccat
     3841 atttgttgca ctgaagcccc attggatcaa cactttcgac gacaaccgct atctgaagtc
     3901 cgaccgcggc atatggcggt agccttcaga cggcatagac aggcaaaacc atgctttcca
     3961 acatcatccc cctttccatc ggtgcggcac tcggtgcaac ggcgcgttgg ctgctcaacc
     4021 tcgccgttcc tgccgccatg tctcccgcca cgggcaacct gtttgccaac tggacgggcg
     4081 cgctgctcat cggcatcttt gccgaaacca tcaatcaccc gcaatggaag ctgctgctga
     4141 ttaccggttt tctcggcagc ctgaccacgc tttccggatt ttcactggaa accgtaaccc
     4201 tgctccaatc gaatcgtcct gcttcagcac ttgccaatat tttcctacac acggcaggtt
     4261 cgctgctgct gacttggctc gggctgaaga taggggcggc agtcaaataa ccgcaacaaa
     4321 ttgcttcctt tggttttgac acaaactata caagtcctaa catcttaatc atggcgcagc
     4381 atataagcgt ggatttgcag cccaatctat actttctgat tctattcatt aagccattat
     4441 gcttattcct caatcacggt ttccaccacc gaatgaatac ttttaggcaa ttgcaaaaaa
     4501 gcattccaag caattaaagc agttttaaaa gtatgcagcg aacagcttaa atcaggccag
     4561 ccgtcttctt catcaaattc gttgttatag aaaactactt tttcagcaga aatatcccag
     4621 caaaaacctt gtccacctcc tgaataaact tcattgggat tattttctgc ttgatggata
     4681 aattctaaat tttcatctat gtctttttgt agataaaggt ctcctgttaa aacataaatt
     4741 aagtatccat atattctatc tttaattatt tcattatttt cttcattttc ttcatctaaa
     4801 aatatacatc tttttaatag ttccccatta aattcataaa catcgtaata agctttaaaa
     4861 atagatatca tatcatttcc ttatcttcca taaatcggct tcggttgttt aaataccgcc
     4921 cgtgccaatt accgttctca aattcaaatt gggcttttag gtctataaca aagaaaaagc
     4981 cttggtagga taaacctacc aaggcttttt cttaaaaaaa cgctctatta ccgtgcctaa
     5041 aaatgcaaaa aagtaaccgt cataaccctt ttgccaaaat caatatcatc atccgatgaa
     5101 tgaattgacg gtatataaga aagaattaat aagctcataa accggtaaga aaaatcagta
     5161 acaaagatat tcattattaa ccattcttaa aaatgatcat gttcgtttgg gtaattatca
     5221 tacccttttc cctcttatta ctaaagcacg aatgacttta aaggacgcgc ccagagacaa
     5281 gccgaaaagg catatcccat ccgccaaaca aaatgcccgc cgacttactc cggcgggcag
     5341 ctccaacacc ctgtcaatcc ttcatcagcc agtattgcaa accgattaat gtcttgccgt
     5401 ctttaatttc atcgtttgcc aatgcctgac ggacttcctc cttcgacatc aatacggttt
     5461 ccgtaatctc gtcttcgtca ttggcaagcg tactgcccaa gcgtacgcct tccgcctcga
     5521 acagatacat tttttcgttg caaaaaccga ccgccgtata aaaactgtac aacaggcgta
     5581 cactgtcggc aacataaggc gtttcctccg ccaattctcg cagcgcacac gctgccatat
     5641 cctcgcccgc cacatccagc ttgcccgcag gaagttccaa tgtcgcctga tttgccgcat
     5701 accgccactg ccgtaccaaa accactttcc cctcgtccgt aaccgccaac acgcatgccg
     5761 caccgggatg acggatgacg atacgctgcc cttcgttacc gttgggcaac ctgaccttat
     5821 ccttgctgat gctgacgaaa ccgccctcgt aaatggtttc gccgcctaat tttacttccc
     5881 tcaaatccac ttcatatctc ctttccgttt cctattctgc cgttcaatct tcacgcttgc
     5941 cgcgctcgat ttccacgcca acctcgcgca cgccttccaa aatacccggc ttgacgattt
     6001 tcacgcgcac ccacaccgcg ccgaaatatc ccaaaaccaa atcggcaata tattccgcca
     6061 acgcttccaa aagcaggaaa tcctgttctt tcagatgtcg gcgcagcgtt tcgcatacct
     6121 cggcataatg caccgtattg gcaatatcgt cgtccgaacc cgctttctcg ggaacgccga
     6181 tgtccaaatc gacaatcagg gtctgcaacc gttcgcgttc ccagccgtac acgccgataa
     6241 gcgtatctgc cttcatgccg tgcaaaaaga ttttatccat aatccgcccc gttttttgct
     6301 aaaataaccg caccattcta agtaaagtac cgcaaatgtt caacataccg gctgttgctg
     6361 tttcctatct gatcggctca ctttctttcg ccgtcatcgt gtccaagtat tacggcatgg
     6421 acgacccgcg cacctacgga tcgggcaatc cgggggcaac caatgtttta cgcagcggca
     6481 aaaaaaaggc ggccgtgctg acgctcttgg gcgatgccgc caaaggtttg gttgccgttt
     6541 tgcttgcacg cgtgcttcaa gaaccgctcg gtttatccga cagcgcaatc gctgccgtcg
     6601 cactcgccgc gctggtcggg catatgtggc cggtgttttt cggatttaag ggcggcaaag
     6661 gcgtggcaac ggcattgggc gtgcttttgg cactctctcc cgcaactgcc ttggtctgcg
     6721 cgttgatttg gcttgttatg gcattcggct tcaaggtgtc ctcccttgcc gcattaaccg
     6781 ccacaatcgc cgcccccctt gctgcgctgt tttttatgcc ccacctttct tggatttggg
     6841 cgacgctcct tatcgccctg ctggtgttgt tccgccacaa aagcaatatc agcaacctgc
     6901 ttaaaggcaa agaaggcaaa atcggcgaaa aacgctgatt cggcatttgt tttccattac
     6961 gtcatgccgt ctgaaaccat atttccgcgt tcagacggca tttccgcaca aacggctttc
     7021 ataccatgct cagttacaga cacgcattcc acgccggcaa ccacgccgat atgctcaaac
     7081 acttcacctt gtttttagtg ctgcaatatt tcaaccgcaa agacaagccc tactggtaca
     7141 tcgacacgca cggcggcgcg ggtgtgtaca atctcgaagg cagcgaggca caaaaagtcg
     7201 gcgaataccg gcaaggcatc gccctacttc gacaagcaaa aaacctgcca accgagttaa
     7261 acggctttat cgcgcacata caaaaaatcc tgccctcgcc caaactttac tgcggttctc
     7321 cgtggctggc gcaatcgctg acccgtgtcg gcgacaaatt gcgcctgttt gagctgcacc
     7381 ccaccgactt tgtccatctg caaaacaata tgggcgaagc aggattgggc aaacgcggac
     7441 aagtgttgcg cgaagacggt tacaaagagc tgatttccct gctgccgccc ccgccgcgcc
     7501 gcgccaccgt cctcatcgac ccgccctatg aggaaaaaca ggactaccgg cgtgtaacgg
     7561 aaacgctgaa agccgcttta aaacggtttg agtccggctg ttatctcatt tggtatccct
     7621 gcctcagccg cgaagaaagc cgcaagctgc ccgaagaatt gaaaaaactc ctgcccgaca
     7681 actaccttca cgccgaactt cacgtccacg cgccgaaagc cgacggtttc ggtatgcacg
     7741 gcagcggaat gttcgtcatc aatccgccct atctgttggc ggaacaattg gcggcaaacc
     7801 tccccgccct gacgcggctg ctggcgcaag acgaaggcgc gcgctacctt ttggacagca
     7861 aaatccgctg accccgatgc cgtctgaaac ccttcagaca acatctcccg tcaaataaac
     7921 aaaaccggac gatataccgg cagtcggcac ttttaagaat aaatatcccc tcatccgcct
     7981 gccgcgttcc gacttgcccc atgccgtttt tttacactac aatcccctag actatcctgc
     8041 aaaacgcacc aaaggaatac gccacaataa cctgccgcct ttcagacggc acaaaccttt
     8101 ccgaaacgag caaactatgg acacactgac ccgattcctc cccgaacacc tgcaacaaaa
     8161 ccagctgccc gaagcactcg gcggcgtttt actgtccgtt gtttccgcct gcaccgaaat
     8221 caacgccaaa gtccgcctcg gcgcactggc cggcgtattg ggtatggcgg gcacgggcaa
     8281 tattcagggc gaagaccaaa aaaaactgga tgttatcgcc aacaacatca tgattgacac
     8341 actcaaagcc aaccctgccg ttgccggttt ggcaagcgag gaagaagact ctttcgtaag
     8401 tgccggtgaa aacggacgct atctcgtcct attcgaccct ttagacggat cgtccaatat
     8461 tgatgtcaac atttccgtcg gtacaatttt ctccatcctt gaaaaacccg aaggcgcatt
     8521 ggcaaccgaa tcattccttc aaacgggcag acagcagctt gccgctggtt acgttcttta
     8581 cgggcctcaa acccagctcg tattcacatt cggacacggc gtatatatgt tcacgctcaa
     8641 tgccgaaaac gaatttgtgc tgacaaaaga aaaaccgaaa gtacccgaaa gtaccaaaga
     8701 atttgccatc aatatgtcca accgccgcca ctggctgccc cccgtccaac aatacatcga
     8761 cgagctcttg gcgggcgaaa ccggcacgcg cggcaaaaac tacaatatgc gctgggtagc
     8821 cagcatggtt gccgaaatcc accgcatact gatgcgcggg ggcgtgttca tgtatccgca
     8881 agacaaacgc gacccgtcca aacccggcaa actgcgcctg atgtatgaag ccaatcctat
     8941 ggccctgata ctcgaacaag ccggcgcatc ggcaagcaac gcccgtcagg acatactcgg
     9001 catccgtccc gaaagcctgc accagcgcgt cgccgtcatt atgggcagca gcgaagaagt
     9061 ggattacctc aaccgcctgc attcaaaata aaaggcagcc tgaccgataa agaaaggcgg
     9121 cagcgcgcat ccgacgctgc cgccgatatt tcgtttatag ccttaaaaag atagaaaacc
     9181 acctttccga acggcaaaaa tgccgtctga aaagttttca gacggcattt taaaaattaa
     9241 agcccgaatt tatttgcccg ctgctttttg caggatggcg tacaactcgt ctttcagttt
     9301 caatttggct tttttcagct catcgatggt ttccgcaccg ctggtaaccg gattgttgac
     9361 cagaccggta attttatcgt ccagctcgtt gtgttcgtcg aacagacggg cgaagcggga
     9421 attttcctgt ttcaatttgg aaatcaaatc acggtattct gggaacatat cgctttcctc
     9481 tcttggttta cggttaaaaa gtcaaactga atctttacat taattataac aaagataata
     9541 tttttatcca gtgttttatc ggatacggca aaattttctt ttaacaccgc cgtccggttg
     9601 ataaaaacgt tgacaatatc acgctttacg cataaatttt cagcctttcc ta
//
LOCUS       DAWEWP010000068         9557 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWEWP010000068 DAWEWP010000000
VERSION     DAWEWP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.068"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            41..196
                     /locus_tag="WGN53_001191"
     CDS             41..196
                     /locus_tag="WGN53_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140186.1"
                     /translation="MHKLCLATSRSDDFIRKTAASGTISNRPTEDGILSTRPAAYAAE
                     EPLYQAS"
     gene            205..1254
                     /locus_tag="WGN53_001192"
     CDS             205..1254
                     /locus_tag="WGN53_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0140187.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKGETRKTATQAY
                     GQKASDGIKPQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGEYRRKKA"
     gene            complement(1323..2864)
                     /locus_tag="WGN53_001193"
     CDS             complement(1323..2864)
                     /locus_tag="WGN53_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ0140188.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGTSAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYGVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(3157..4512)
                     /locus_tag="WGN53_001194"
     CDS             complement(3157..4512)
                     /locus_tag="WGN53_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ0140189.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVAGKISIYETRGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPARPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGTGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKDTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            4709..5530
                     /gene="nadE"
                     /locus_tag="WGN53_001195"
     CDS             4709..5530
                     /gene="nadE"
                     /locus_tag="WGN53_001195"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ0140190.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGIHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(5598..6071)
                     /locus_tag="WGN53_001196"
     CDS             complement(5598..6071)
                     /locus_tag="WGN53_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0140191.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            6369..6701
                     /gene="trxA"
                     /locus_tag="WGN53_001197"
     CDS             6369..6701
                     /gene="trxA"
                     /locus_tag="WGN53_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ0140192.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(6754..7701)
                     /locus_tag="WGN53_001198"
     CDS             complement(6754..7701)
                     /locus_tag="WGN53_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0140193.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            8029..9417
                     /locus_tag="WGN53_001199"
     CDS             8029..9417
                     /locus_tag="WGN53_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0140194.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPTDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGRGERRYKDR"
BASE COUNT         2332 a         2660 c         2534 g         2031 t
ORIGIN      
        1 ttgaaagatt ctacccccgc acgcaaaccg atttcaaccg ttgcacaaac tttgcctcgc
       61 cacaagccgc agcgacgatt tcatccgcaa aaccgccgca tcaggtacaa tatcgaaccg
      121 tccgactgag gacggcattt tatcaacccg tcctgccgca tacgccgcag aagaaccgct
      181 ttatcaagcg agttaggaaa aatgatgtcc aaacagccca ccagcaaacg ccaatggcgc
      241 gacggcgcag ccccgtctgc caagaaaacc gccaaacctt tcaaaagcaa agcccgtccc
      301 aaaggcgaaa cgcgcaaaac cgccacccaa gcttacggac aaaaagcttc agacggcatc
      361 aagccgcaaa acgcccccaa acagcgcgcc gccaaagcca aaaaactcgt cgtccgcaat
      421 cccaaccaaa aaattatgga acacgcgcgc gatttgaaag aacgccgcag cgacctgtcg
      481 cgcatggaac ccgaacgcct gcaaaaagtg cttgccgcgt ccggcgtcgg ctcgcgccgc
      541 gaaatggaag aatggattac caacggctgg ataacggtca acggcaaaac cgcgcaactg
      601 ggcgacaaag ttacccccga cgaccacgtt accgtcaaag gcagcatcat caagctcaaa
      661 tgggcggacc gcctgccgcg catcatcctg tattacaaac aagaaggcga aatcgtttcc
      721 cgtgacgacc cgcaaggccg cgtcagcata ttcgaccgcc tgccgcaggc cgccagcagc
      781 cgctgggtcg ccatcggacg cttggacatc aacaccagcg gacttctgat tcttaccacc
      841 tccggcgaac tcgtccaacg tttcgcccac cccagcttcg aagtcgaacg cgaatacgcc
      901 gtgcgcgtct tgggcgggct gaccggcgaa caaatgcgcg tcctcaccga agaaggcgtg
      961 atgctcgaag acggcttggc aaaagtcgaa cgcatccgcg aacaaggcgg cgaaggcgcg
     1021 aacaaatggt acaacgtcgt gattaaagaa ggccgcaacc gcgaagtgcg ccgcattttt
     1081 gaaagccaag gactcaccgt cagccgcctc gtgcgcgtcg gcttcggtcc catcggactg
     1141 cccaaccgcc tcaaacgcgg gcagttctac gaactcaacc ccgccgaagt cgccaacatc
     1201 ctcaaatggg cggaaatgct gctgccgggc gaataccgcc gcaaaaaagc ctaaacccgc
     1261 caaaacacaa aaatgccgtc tgaaacatct gctgtttcag acggcatttt attcgggcgt
     1321 tttcaggaga aaaggtcgag tgctttgaca aagaccatca ccacgccgta ggcgagtggt
     1381 atgacggcca atgcccaacg ccaccagacc gatatgccgc cgccggaaac tttttcgccc
     1441 acaaggtaag cgtcggatat ggcggtttcg tcgtcggggt tgccgctgtg tgcggcggtt
     1501 ttgatgtctt tttcgtggtg tttttcgtgt acggatttga cggcgaggtt gcacaacaaa
     1561 ccgataatca gcaggcacgc catgatgtac atggttacgc cgtatgcctg tgccgccggt
     1621 atgccgctgt cgatttggct ttggcgtatg taattgacca gtaccgggcc gatgacggcg
     1681 gcggttgacc aggccagcag gatgcgcccg tgaatcgcgc cgacctgata ggtgccgaac
     1741 aggtctttca ggtaggcggg aatggcggca aatccgccgc cgtacatgga aataatcacg
     1801 caaaagccga tgatgaacag ggctttgctg ccgccctcgc cgatggaggg aacggcgaaa
     1861 tacagcagcg agccgagtac gaagaagatg gtgtaggtgt ttttgcgtcc gattttgtcg
     1921 gaaacgctcg accacaaaaa gcgtccgccc atgttaaaca ggctcaggag gctgacgaag
     1981 cctgccgccg cacctgcgcc gactgctgcc tgcctgccta tggaggtttc ggaaaagagt
     2041 tcctgaatca tcacggatgc ctgacccaat acgccgatgc cggcggttac gttcaggcac
     2101 aatacccaga acaacagcca aaactgcggc gttttcatgg cttgggacac gttgacatga
     2161 ttactgctga ccagcttgtt ttgcgttttc ggcgcggtat agccttcagg tttccagccg
     2221 tcggcaggta cgcggatggt aaacgcgccg aacatcatca gtgcgaggta aagcaggccc
     2281 aatacggcaa aggtttcggc aaccccgacc gaagcggcgt ttgaaaaggc gttcatcagt
     2341 gatacggaaa gcggagaggc cagcattgcg ccgccgccga aacccataat cgccaaaccg
     2401 gtcgccatac ccggcttgtc gggaaaccat ttcatcagtg tggaaaccgg cccgatgtag
     2461 cctaagccta agcctacgcc gccgatgacg ccgttgccca aatagagcag gaagaggttg
     2521 tgcgtacgca cgccgaatgc ggatacgaag aagcccaggc tgaaacagca ggcggcggca
     2581 aatatggctt tgcgcggacc tacccgttcc atccatgtgc cgaacagggc agccgacgtg
     2641 cccagcatcg cgagtgcgat actaaaaatc caacctacgg tcgtcagctt ccaatctccg
     2701 gccgccgatt cggttatgcc gataagtttg gtcagcggcg cgttgaatac ggaataggcg
     2761 taaatctgcc cgatggcaag gtgcaccgcc aatgctgcgg gcggtacgag ccaacggttg
     2821 aaacccggct tggcaatgct tgcctcacgg tctaaaaact tcataacatc ctctttctgt
     2881 cagttgaaaa ataaaatttc atttgcccaa tggaaactta ttgaaaatta taaaaaaata
     2941 tcggatcggt tttttatccg ccccaagatg ccccgtctga aacatttcgg gtgtacggaa
     3001 aggtttctgt tttttccgac aaattcctgc ggcttttcgc ttccggattc ccgctttttc
     3061 aggaatgacg aattaaagat tatcttaagg tcaagggact ggattcccgc tttcgcggga
     3121 atgacggcgg agggggagcg gtttttccga ttgggtttaa atgcaatcga acaaatcctg
     3181 ctgcccttgt tctttgctta cgcgcacgtc ggtttcgccg tcggcgaagg taatgtgcag
     3241 cttctgcccc tgcttcaaaa catcggcgtt gcggatgact tgtccgcgtg tatctttgac
     3301 gacggaaaag ccgcgttcca aaatgtgctg cggcgaaacg gcttcgagca atgcggcttg
     3361 ggcggtcagg ctttggcggc ggtgggtaag cagttggctg aaggcggcgg gcaatgccgt
     3421 ctgaaggcgg acgatgttgt ttttgcaaac ggaaacatca ggacggtaat gttgcagggc
     3481 ttgggtttgg cgttcaaaac gggcggtgtg ggcacggagg ttttgcttca tagagtaaga
     3541 cagcgtttgc gccagcttgc cgattgaagc gcgctgctca tcaagttttt ggcgcggatg
     3601 acggatttgc cgcgcaagcc agtcgagttt ttggctggca tcgaaatagc gttgttccaa
     3661 aacggttttc agacggcctt gcgcctgagc gagacgatgt agcgattctt ggcggttggg
     3721 gctgaccagc tccgccgcac cggtcggcgt gggcgcgcgc acgtcggcga caaaatcggc
     3781 aagcgtgaaa tcggtttcgt gtcccacgcc actgacgacg ggaaccgtgc aggcttcgat
     3841 tgcgcgcacg accggttctt cgttaaacgc ccacaagtct tcaatgctgc cgccgccgcg
     3901 acagacaatc aacacgtcgc attcggcgcg ttgcgaggcg gttttaatcg cttgtgcgat
     3961 ttgtaactcg ctgcctgtgc cttgaacagg tgtcggataa acgataacgg ggatttcggg
     4021 tgcgcggcgt ttcaaggtag taacgacatc gcgcaaagcc gccgccgcca gactggttac
     4081 gatgccgata cattgcggtc gggcgggcaa aggtttcttg cgttccgccg caaacgcgcc
     4141 ttccgcctgc aactgcgcct tcaaccgctc gtaggcttcg taaagctgcc ccaagccctt
     4201 gagccgcact tcgttcacgg taatctgaaa ttcgccccgc gtttcataaa tactgatttt
     4261 gcccgccact tcgatatggt cgccttcttt caaaggtttc gccaaacgcg ccgccgcacc
     4321 cttgaacatc gcgcaacgca cctgcgcgcg gctgtctttg agcgagaaat aataatgccc
     4381 gctggcggca cgggtcaggt tggacacttc gcccgcaatc cacagaccgg caaggtggtt
     4441 ttccaaaata cttttggcaa atgcgttcaa ctcggaaacg gacaacacgt cagaatggaa
     4501 aaaatcagac atcgaatcaa tcaaatagta aaaaatatga atatgttttg aagcccaagg
     4561 cggcatagag ccgcctaaat tgtcaacaat attataacac gcgccatctt gccgcccgcc
     4621 ttttcccata tgactttttt aagcggggaa tgggaaaaat attcatcaac ctgcctgcaa
     4681 tctattcaaa ttgaaccgcc gacaagctat gatgcggata ttttcgacag gaggaaaaat
     4741 ggatacgcag gcagttatca cacatatcgc ccgatggttg gacgaatacg ccgcacgggc
     4801 aaatgcaaaa gggtttgtcg tgggcgtttc cggcggcatc gattccgccg tcgtctccgc
     4861 actcgccgcc cgcaccggcc gccccacgct gcttctggat atgccgatac gccaacaccc
     4921 cgaccagctt gagcgggcaa ggctgcacat ccgcaatctg caacggcaat atgccaatgt
     4981 aagcgcgcaa acggtcgacc tgaccgacac cttccagacc tttgaacaaa ccgtcggcat
     5041 tcatcagacg gcatttgaca atcagccgct ttccctcgcc aacgccagaa gccgcctgcg
     5101 tatgctgacc ctgtactact acgggcagat acacggactg ctggttacgg ggacaggtaa
     5161 taagattgaa gatttcggcg tgggcttttt tactaaatac ggcgacggcg gcgtagacat
     5221 cagcccgatt gccgacctga ccaaaacgca ggtttaccgg cttgccgaag cattgggcgt
     5281 ggacgaggcg attcaaaaag ccccgccgac cgacggcctg tgggatacgg aacgcaccga
     5341 cgaagaacag atgggcgcaa gctatcccga actggagtgg gcaatgggcg tgtacggcac
     5401 gcgcaaaccc gaagattttg aagggcggca gcgcgaagtt ctggaaatct atacgcggct
     5461 tcaccgcgcc atgcagcaca aaatcaaccc gattcccgtg tgccgcatcc cgcccgaatt
     5521 gttgggctga acgtgaaaat gccgtctgaa gcggaaaacc gtgtttcaga cggcatggaa
     5581 atatccggac gcgctcctta agaatcgagt acgcgggcaa acagaatatc gttttccaaa
     5641 tgaatgtggt cgttcaaatc ctccaccatt tctttcgcca gcgcgtaaag ccgcgtccag
     5701 cttccgcaag ccccttcggg cgcttggaaa ttgtcggtca gctctttgag ccgtgcgatg
     5761 gcgcggtcgt gttcttcgtg ttcgtgcatc atcacgctga tgggcattgc cgcaccgcgt
     5821 ccgacaccct gattaatcat cggaaacagc atcctttcct ctttcatcat atgcatcagc
     5881 agttcgttct gcatataggc aagcagcccg gcaatttccg ccggaaaggt atcggcatgg
     5941 acttgggcca ctttctgagc cagcggcacc aattcttcaa attgtgcgcg gtggacattg
     6001 tggtagcgtt gcaggatatg atcgacggtt gcaccaaagg gggcggtctc ccaaacggaa
     6061 aaatcagtca tcgcagtgtt ccttttacag ggtttcgggt ttggttttga acattcatac
     6121 tttaagaatc aattcaaacg gagcatacac cgcccgcgcg cttctgtaca gcctcaaacg
     6181 tattccttac attttgataa taaaagtaat tttcagaaat aaaatactgt ccgaaccgtt
     6241 ttttagaatt tgcaaaggcg attggggcgg tacagaaaaa ctattatccc gcccgcccac
     6301 ttgaaatttt tatgcccaag ccctatcctg cacgctatcg tgccaatccc aaccgaaaag
     6361 gaaaaataat gagcagcgaa ttgattgtac acaccagcga tgccgctttt gaaaaagacg
     6421 ttttaaatgc agatatcccc gtcctgctgg acttttgggc tccgtggtgc ggcccctgca
     6481 aaatgattgc cccgattttg gacgacattg ccgccgaatt tgaaggccgt ctgaaagtgg
     6541 tcaaaatcaa catcgacgac aacgaagcca ccccgtcccg tttcggcgtg cgcggcattc
     6601 cgaccctgat ggtgttcaaa aacggcgaag tcgtcgccac caaagtcggc gcattggcaa
     6661 aaggtcagct gaccgccttt gtcgaagcct ctatcgcctg ataaagcgca atcgaaaaag
     6721 ccgccggaag attccggcgg ctttttcgca cccttaagat ttgtggcgga tttcccagca
     6781 cctatggatt tttttgttgc ggaaatcttc gggaacggat tgtttggaaa tgtctttgac
     6841 ggcgtattgt tccgatacca agtcgtctaa gacgaagctg cgcaggttgt tggaaaagta
     6901 caaaatgccg tctgaagcga gcagcttcac cgcgccgtca atcagctttt tgtggtcgcg
     6961 ctggatgtcg aggatgtcgg acattttctt gctgttggaa aaactgggcg ggtccatcac
     7021 aatgaggtcg aaccgcctgc cttccccata tgccgtctga agatattgga acacgtcggc
     7081 gcggacgatt ttatgccgtt ccgtatcgat gccgttcaat tcaaaattgc gtctcgccca
     7141 atcgagataa gtgttagaca aatcgacagt ttcgctggat gccgcgccgc cggtggcggc
     7201 atagacagtg aagctgccgg tgtaggaaaa caggtttaaa aaacgtttgc ccgccgccgt
     7261 ttcgccgact tttttgcgcg tgtttcgatg atccaaaaaa agccccgtat ccaaatactt
     7321 atcaaggttg acccaaaact tgcggccgtt ttcggtgatg acgaaatcgt cgcccgcctt
     7381 gccggttttt tcgtattgct gcaaaccttt ttggcgttcg cggcgtttga ggcggatttg
     7441 ttcgggttca aaaccggtaa cgaaagcgac ggcttccaag acttcggcaa gccacgcttc
     7501 gtattcttcg ggccgcatca gccagccggt atcgtattcc tgaaggtgga ttcgatcgcc
     7561 gtaaacatcg gcggcaaagg ggaattgggg gatgtcgcgg tcgtaaatgc gccaggcttc
     7621 aatgccgttg cgtttcgccc atttcataag gtgtttgatg tttttgccca agcggttggc
     7681 aaacggtgtg atgtcggtca ttggtttcag gcggaataaa gtggaaaacg gcaattttac
     7741 tgtaattaac gcccgattgc ttgaccgttt cgggcaaacc ctataccatc cgccgcttat
     7801 cttgtcatac gaagccatcg ccttccaacc taaaccgccc ttacgggcgc gtttcttttg
     7861 ttgctttgat tttgcaaagc ctttctgtgc aggttgtcgt cgatgtaaac cacaagcaag
     7921 ccgcttgcga caaccctgta acttcacatt ccccgtatcg ttacccttcc ctgcttcagg
     7981 ccgtctgaac ctttcggacg cgggcgttgt tgtcttccaa ggatagccat gtctattaaa
     8041 tttgccgatt tgaaccttga taaaaacatt ttgtccgccg tcagcagcga gggttacgaa
     8101 agcccgacgc cgattcaggc gcaggcgatt ccgtttgctt tggaaggccg cgacatcatg
     8161 gcttcggcgc aaaccggctc cggcaaaacc gccgcctttc tgttaccgac tttgcagcga
     8221 ctgaccaaac gcagcgaaaa accgggcaaa ggcccgcgtg ctttggtgtt gaccccgacc
     8281 cgcgaattgg cggcacaagt ggaaaaaaac gcgctggcgt atgccaaaaa tatgcgctgg
     8341 ttccgtaccg tcagcatcgt cggcggcgca tcgttcggct accaaacccg cgccctgagc
     8401 aaaccggtcg atctgattgt cgccacgccg ggccgcctga tggacctgat gcaaagcggc
     8461 aaagttgatt ttgaacgttt ggaagtgctg attttggacg aagccgaccg tatgttggat
     8521 atgggcttta tcgacgacat cgaaaccatc gtcgaagcca ccccgaccga ccgtcagact
     8581 ttattgttct ccgccacttg ggacggcgcg gtcggcaaac tggcacgcaa actgaccaaa
     8641 gaccctgaaa tcatcgaagt cgaacgcgtg gacgaccaag gcaaaatcga agaacaactg
     8701 ctgtactgcg acgatatgcg tcacaaaaac cgcctgctcg accacatcct gcgcgatgcc
     8761 aatatcgatc aatgcgtgat tttcacgtcc accaaagcga tgaccgaagt catcgcggat
     8821 gaattgtacg aaaaaggttt cgccgccaac tgcctgcacg gcgacatgcc gcagggctgg
     8881 cgcaaccgca cgctgatgga tttacgcaaa ggccgctgca aaattttggt tgccaccgac
     8941 gttgccgcac gcggtatcga cgtaccgacc attacccacg tcatcaacta cgacctgccg
     9001 aaacaggcgg aagactacgt ccaccgcatc gggcgcaccg gccgcgcagg ccgcacgggt
     9061 attgcgatta cgtttgccga agtgaacgaa tacgtcaaag tccacaaaat cgaaaaatac
     9121 attaaccgca aactgcccga actgaccatc gaaggcatgg aaccgacccg caaacgcaaa
     9181 tccgcaggcg gtaagccaaa aggcaaaggc ggctggggcg ataggaaatc cggcggccgg
     9241 cgcggcgatc ataaaccggg caaagaaggc ttcggcggca aaacgcgcgg cgaaggtttc
     9301 aagaaagaag gctttaagaa agacggtttc aaaaaaaccg gcgaaggctt caaaggcaaa
     9361 cgtaaagccg gcgattcttt tgcaggcaga ggcgaacgcc gttacaaaga ccgctaagcc
     9421 ccaacctgcc tcataaacca atgccgtctg aaaccgattt cgagtttcag acggcatttt
     9481 tgcaatgttt caacaccgcc cggctttgat acccaaagga ttaatctgca atatagtgga
     9541 ttaacaaaaa ccagtac
//
LOCUS       DAWEWP010000069         9418 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWEWP010000069 DAWEWP010000000
VERSION     DAWEWP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.069"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            134..343
                     /locus_tag="WGN53_001200"
     CDS             134..343
                     /locus_tag="WGN53_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ0140195.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            432..1646
                     /locus_tag="WGN53_001201"
     CDS             432..1646
                     /locus_tag="WGN53_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ0140196.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1733..4312)
                     /gene="clpB"
                     /locus_tag="WGN53_001202"
     CDS             complement(1733..4312)
                     /gene="clpB"
                     /locus_tag="WGN53_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ0140197.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSCLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMSIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4573..5583
                     /gene="trpS"
                     /locus_tag="WGN53_001203"
     CDS             4573..5583
                     /gene="trpS"
                     /locus_tag="WGN53_001203"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ0140198.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5589..5930
                     /locus_tag="WGN53_001204"
     CDS             5589..5930
                     /locus_tag="WGN53_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140199.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6250..6615
                     /locus_tag="WGN53_001205"
     CDS             6250..6615
                     /locus_tag="WGN53_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140200.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAKEAVTEAAKDTL
                     NKAADATQEAADKMKDAAK"
     gene            complement(6824..8332)
                     /gene="ppx"
                     /locus_tag="WGN53_001206"
     CDS             complement(6824..8332)
                     /gene="ppx"
                     /locus_tag="WGN53_001206"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ0140201.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPDGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAAPHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(8449..9180)
                     /locus_tag="WGN53_001207"
     CDS             complement(8449..9180)
                     /locus_tag="WGN53_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0140202.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLHLSLGYKNR
                     SVTRSFGIVPFVGQNLLGGSRYNFVGGFNADFSQHLSERWRLTLNAGNMWKHYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHDLTKEAEQASVRKGLRIGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTIVYRFPRKDHEYQANLSFWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNTQIFMSVEKDFK"
BASE COUNT         2173 a         2440 c         2485 g         2320 t
ORIGIN      
        1 tgcggcttcg tcgccttgtc ctgatttttg ttaatccgct atatcgattt gaatttttca
       61 gaaaatgaag cggacgtttg ggcggaggca acttgtttga taagatagca atatttcaaa
      121 acggagaaag atcatgcctt atgtcaatat taaagtaacc ggcggcaagg aagcaccgac
      181 tgccgcgcaa aaagcggaac tgatcggcgg cgtaaccgaa ttgctggcac gcgtgctggg
      241 caaaaatccc gaaacaacgg ttgtcgtgat tgatgaagtg gataccgata actggggaat
      301 aggcggcaaa agcgtcagcg aacggcgcaa agagggcagg taaaaagcct gaaaatctcg
      361 gtttgatgct ttaaattccg cgtgaaaaag agtacattcc cacccattgc ccaaaattta
      421 cggaacacat catggataaa tttcccaagt ctgcaaagct cgatcacgtc tgttacgaca
      481 tacgcgggcc ggttcacaaa aaagcccttc agttggaaga ggagggcaat aaaatcctta
      541 aactcaatat cggcaaccct gcgccgttcg gctttgaagc ccctgatgaa atcttggtcg
      601 atgtcatccg caacctgccg acttcgcaag gctattgcga ttctaaaggg ctgtattccg
      661 cccgcaaagc cattgttcac tactatcaga ccaaaggttt gcgcgatatt acggttgatg
      721 atgtctatat cggcaacggc gtgtccgagc tgattacgat gtctatgcag gcattgctca
      781 acgacggcga cgaaatcctg attcccgcgc ccgactaccc tttgtggacg gcggcggcaa
      841 cgcttgcggg cggtacggta cgccattatc tgtgcgacga agaaaacggc tggttcccca
      901 accttgccga tatggaagcc aaaatcacgc ccaaaaccaa agccatcgtc gtcatcaatc
      961 ccaataatcc gacaggcgcg gtgtacagca gggaaatcct gttggaaatc gccgaactgg
     1021 cgcgcaagca cggtttgatt attttcgccg acgagattta cgataaaatc ctttatgacg
     1081 gcgcggttca ccaccacatc gccgcgcttg cccccgacct tttgacggta acgttcaacg
     1141 gtttgtccaa atcctaccgc gtcgccggct tccgccaagg ctggatggtg ctcaacgggc
     1201 cgaaacatca cgcaaaaggt tacatcgagg gtttggatat gctctcgtct atgcgcctgt
     1261 gtgccaatac gccgatgcag cacgcgattc agacggcatt gggcggctat cagagcatca
     1321 acgaatttat tttgcccggc gggcggcttt tggaacagcg caacagggcg tgggaactgg
     1381 tcagccaaat ccccggcgtg tcctgcgtca aaccgatggg cgcgatgtat atgttcccaa
     1441 aaatcgatac cgaaatgtac cgtatccacg atgacatgaa attcgtttac gatttgctgg
     1501 tgcgcgaaaa agtcttgctg gtacagggaa cggggtttaa ttggattaaa cctgaccatt
     1561 tccgcattgt cacgctgcct tacgtccatc agattgaaga ggcgatgggc aggttggcaa
     1621 gattcctgca aacctaccgc caataagggg acggtttgtc tgccgaggat aaaaaatgcc
     1681 gtctgaaacg gagattcccg tttcagacgg cattttcaac agcaggaacg aatcaggcaa
     1741 atttcagtct gtcgccgtcg gcttctaccc tgatttcgct ttcgggcgca tagtttccgg
     1801 caagcagggc ttttgccagc gggttttcga tttccgactg gatggcgcgt ttgagcggac
     1861 gtgcgccgta aatcgggtcg aaaccggctt tggcgatgat gtccagtgcg gcatcggaaa
     1921 cagtcaggcg caggttttgt ttttccaaac gtttttccaa acctttgagc tggattttcg
     1981 caatgctgcg gatgtttgcc tgatccaaac cgtggaacac gaccacttcg tcgatgcggt
     2041 tgatcatttc aggacggaaa tgttctttca catcctccat cacaacttct tttaccgctt
     2101 cgtaatcctg aatgctcatt tgttggatgt gctggctgcc gatgttggaa gtcatcacaa
     2161 tcacggtatt tttaaaatct accgtcctgc cttgcccgtc ggtcaaacga ccgtcatcca
     2221 atacttgcag caggatgttg aacacatcgg gatgggcttt ttccacttcg tccagcagaa
     2281 tcacgctgta cggtttgcgg cgcacttgtt cggtcaggta gccgccttct tcgtagccga
     2341 catagcccgg aggcgcaccg atcaggcgtg caacggcgtg tttttccata tattcggaca
     2401 tatcgatgcg aatcagatga tcttcgctgt cgaacagaaa gcctgccagg gctttacaca
     2461 actcggtttt acccacgccg gtcgggccca agaacaggaa gctgccgtaa ggcttgttcg
     2521 gatcggcaag accggagcgg ctgcggcgga tagcgtcgga cacggcacgc acggcttcgt
     2581 cctgtccgac cacgcggcgg tgcaatactt cttccatttt cagcagtttg tcgcgttcgc
     2641 cttccatcat tttggatacg ggaatgccgg tcatacggga aaccacctct gcgatttcct
     2701 ctgcgccgac attattacgc aagagtttgt ttgccggttt tgtgctgtcc gtatctgccc
     2761 gttcggcggc tgcacgctgt ttttccaaat gctccaaatc ttcatacatc aattttgaag
     2821 ccagtgccaa atcgccttgc cgttttgcct gttcgatttt aattttgact tcgtcaattt
     2881 gtttcttaat attagcagca ccgtctgaaa ttgctttttc ggctttccag atttcgtcta
     2941 aatcggcgta ttctttttgc agaccgttga tttcctcgtc tatcagttcc aaacgttttt
     3001 tgctggcatc gtctttttct ttttcaacgt gcgccttttc catccgaagc tgaattagac
     3061 ggcggtcgat tttgtccatt gcttccggct tggtttcttt ttccatcttg acacggctgg
     3121 cggcttcgtc aatcaaatca atcgctttat caggcaggaa gcggtcggta atgtagcggt
     3181 cgctcaactc cgctgcggca acgatagcag ggtcggtaat atcgataccg tggtggattt
     3241 cataacgctc ctgtaaaccg cgcaagatag cgatggtgtc ttctacactt ggctcgccaa
     3301 ccaatacttt ttggaagcgg cgttcgagtg ccgcgtcttt ttcgatgtat tggcggtatt
     3361 cgtccaaggt ggtcgcgccg atacagtgca attcgccacg tgccaaagcc ggtttcagca
     3421 tattgcccgc atccatcgca ccgtcggttt tgcctgcgcc gaccaaagta tggatttcgt
     3481 cgatgaaaat cagagtgttg ccgtcgtctt tcgccaagtc gttcaacacg cctttcaagc
     3541 gttcttcaaa ttcgccgcgg tatttcgcgc cggcaatcaa tgccgccaag tccaaaacca
     3601 gtaggcgttt gttgcgcagg gattcgggta cttcgccgtt gacgatgcgt tgtgccaaac
     3661 cttcgacgat ggcggtttta cccacgcccg gttcgccaat cagcacaggg ttgtttttgg
     3721 tacggcgttg caatacctga atcgcacggc ggatttcgtc gtcacggccg ataacggggt
     3781 cgagcttacc gtctcgggcg cgttgggtca gatcgagcgt atattttttc aaagcatcgc
     3841 gttggtcttc ggcattggca tcgttcacgt tttgtcctcc tcgtaccgcg tcaatcgcgg
     3901 cattgatgtt ttgttcggtc gcgccggctt ctttcaaaat tttgccggcc gcatcgttct
     3961 gctgcaccaa ggcaagcagg aaaagttcac tggcgatata ggcatcgccg cgtttggttg
     4021 cggctttgtc catcaggttc aacaccgcct gcaattcgcg gctgggcaga atgtcgccgc
     4081 cctggccgga cactttcggc aggctgttca aatgctgctg caaacgctgt ttcacctgcg
     4141 gcacgttcac gcccgcatga gccaagagcg cggcagcacc gctgttttgg tcgtcaagca
     4201 gggctttcag cacaaagccc gcttccagac agctgccgtc cgcagccaac gccaaactct
     4261 gagcttctgc aagggcctgt tggaatttgg cggttaattt gtcgtatcgc atttttgttt
     4321 ccttttcaaa atgtccgctg tcgaagccta tatgtgcata attgtggata actcaagttc
     4381 tgttttctgt ttttctatat ttaattcgat atatcattga atttaaagta tataaaaatg
     4441 tataataatg tgtataacta tatcttctta atatggaaaa gtctgttgtc ggctggatgt
     4501 aggtggcaaa tcgggtataa tcggcgcatc tttttccctt tcagacggca ttgatgccgc
     4561 aaggacattt ttatgagcaa aaaacgagtt ctgaccggcg taaccaccac cggcatcccg
     4621 catctgggca actacgtcgg tgctatccgc cccgccgtcc gcgcggcgca aaaccctgat
     4681 accgaatcct tcctcttcct cgccgattac cacggtatca tcaaatgcca cgagccggag
     4741 atgattcacc aatccaccca agccgttgcc gccacttggc ttgcctgcgg actcgacccc
     4801 gagcgcacca ccttctaccg ccaaagcgac attcccgaag tgatggaatt gaactggatt
     4861 ctgacctgca tcaccgccaa gggtttgatg aaccgcgccc acgcctacaa agccgccgtg
     4921 caggcaaatg cggaaaacgg gcaggaagac cccgatttcg gcgtggaaat gggtttgttc
     4981 agttatccga ttctgatgac tgccgatatt ctgatgttca acgccaacga agtccccgtc
     5041 gggcgcgacc aaatccaaca cgtcgaaatg gcgcgcgaca tcgccggccg cttcaatcac
     5101 cgcttccgag aactcttcac cctgcccgaa gtgaaaatcg acgaaaacgt cgaactcttg
     5161 gtcggtttgg acggacgcaa aatgtccaaa tcctacggca acaccattcc gctttgggaa
     5221 aacgacaaaa aaacccaaaa atcggtcaac aaaatcatca ccaatatgaa agagccgggc
     5281 gagccgaaac agcccgacga aagcccattg tttgaaatct acaaagcctt ctccacgccg
     5341 tctgaaacgg tggaatttac gaaaatgctt gccgacggcc tggcttgggg cgaggcaaaa
     5401 aaacttttgg cggcgaaaat caacgccgaa ctcgccgaac cgcgcgaacg ctacaacgag
     5461 ctgaccgccg acccttcgca aatcgaagag attttacagg caggtgcgca aaaagcgcgt
     5521 aaagaagcac gcgaattgtt ggacaaagta cgcgatgcgg tcggcatccg cccgttgaaa
     5581 taaacccgat gccgtccgaa cctcttaaag gcttgccgga acaaaacgtc gtccggctga
     5641 ccggcaagca tcccaacgac ttggaagccg tcagcggcaa atgcatggaa accgacgata
     5701 agggcggtcc ggtaggctgg gcggaaaacg gcgtgtgcca taccttgttt gccaaactgg
     5761 tgggcaatat cgccgaagac ggcggcaaac tgacggatta cctgatttcg cattccgccc
     5821 tgcaacccta tcaggcaggc aaaagcggct atgccgccgt gcagaacgga cgctatgtgc
     5881 tggaaatcga cagcgagggg gcgttttatt tccgccgccg ccattattga ggtattcgga
     5941 catcccggaa tatatttggg tttttcaaac cttgcgaaaa aaagtccgca ccgcagggaa
     6001 actcaaaagg agggggatat gtgttacaat tttccgaact gttttcataa aatagttttc
     6061 ggacgtgttt caatatggca ttgatgccgc cttattgttc gagaaaaaaa cctttatatt
     6121 taaatataat gggttttaac taaacgggaa accgttttct ctccggccga tgggcaaaat
     6181 cagccgattg atggaacacg gttcgatttt taagcaaaac cttaatcaac aactcaagaa
     6241 ggataagaca tgaaaaaatt attgattgcc gcaatgatgg cggctgcctt ggcagcttgt
     6301 tcgcaagaag ccaaacagga ggttaaggaa gcggttcaag ccgttgagtc cgatgttaaa
     6361 gacactgcgg cttctgccgc cgagtctgcc gcttctgccg tcgaagaagc gaaagaccaa
     6421 gtcaaagatg ctgcggctga tgcaaaggca agtgccgagg aagctgtaac tgaagccaaa
     6481 gaagctgtaa ctgaagccaa agaagctgta actgaagcca aagaagctgt aactgaagca
     6541 gctaaagata ctttgaacaa agctgccgac gcgactcagg aagcggcaga caaaatgaaa
     6601 gatgccgcca aataatttgt tgccttggca aaaatgatgg gatgccgcct gccggcaacc
     6661 caaacgaaac cgcctgaaga ttttcaggcg gtttttgagt tgtatgcccc ttgtttttta
     6721 cgcctgcatg accgttccga gcagatgtgc cgcgcatatc gggatgttgc ggacaggcaa
     6781 atgccgtctg gacagggttt ggacggcatt tgttccgtaa ggttcagacg acctcgactt
     6841 taaacggcat attgattttt tgccattgga cgctttcata ttccaatgcg tcggcaatca
     6901 ggggatggcg ttccagccat tccctgtcaa tacgcaggat gaagccgcag ctttccgtat
     6961 ccgtgcgcaa ctgcatattt ttcgggaaag acaggtcttg gcgcgaacgg cagaacaggg
     7021 cagcaaggcg cagggacaaa acggcatacc acaacatttc gttggtgccg atgatgccgc
     7081 tcattttttt catatcgccg cgatgaccga tgaccagttg ggcaagtatg gtctgttctt
     7141 tgcgtgagaa acccggcata tcggcgtttt cgaggatgta ggcggaatgc ttgtgatagc
     7201 cggtgtgggc gatgtccaaa ccgatttcgt gcggcgcggc ggcgcgtccg agatactgtt
     7261 gccacaaggc aagctcttga actgtaacgt ttttagcgtg gcagaggctg tccataaagg
     7321 tttgcgcggt ctcggcggtg cgtttcgcct gattgaggct gacgtggtag cggtgttgga
     7381 actcggcaac cgtttgtccg cgcatatctt cgtttaaacc gcgcccgatc aaatcgtaaa
     7441 acacgccgtc gcgcagggcg gcttcggtta cggtcatcct gtcgagtttc atttcctcaa
     7501 acgccgccat catcacggca agtccgccgg caaaaacttc gatgcgttcc ggtttcaggt
     7561 tttcaaattt ggcttttttg accgaaccgg cttcgatgat gcgttcggcg agggcgcgca
     7621 tgcctttgta ggtaatgtcc gcctcttggg gcatttcggc ggcaagcacg tcgcggatgg
     7681 attttgccga acccgatgtg ccgacggcga aatcccaacc ttcgcgcctc atatttttgc
     7741 tgatgcgctg gatttcgttg cgggcggcgg aaatggcaga ttggaagtct ttggcggtga
     7801 ttttgttttg gaagaagcgc aggctgtagg ttacgcagcc caagggcagg ctttcagtaa
     7861 tgtcgggatt cagcgtcgag ccgatgacaa attctgtcga accgccgccg atgtcgataa
     7921 ccagcatttt gccgccgtcc ggggggaggg tgtggatcac gccggtataa atcagccgcg
     7981 cctcttcgcg cccggcgatg atttcgatgg ggaaacccag tgccgcttcg gctttgggaa
     8041 ggaaatctgc gatgtttttg gcgacgcgga atgtattggt tgccacggcg cgtacctgtt
     8101 cagggcggaa gccgcgcagg cgttcgccga attttgccag acagtccaaa gcctgttctt
     8161 gggaagcggc actcaggttt ttctgttcgt ccagtccggc ggcgaagcgc accatctgtt
     8221 tgaacgaatc gatgactttt aattgtccgt tgttgttttc gcaaatctgg aggcggaaac
     8281 tgttggaacc caaatcgacg gaggcgagga tgtttgcggg ggtggtggtc atggcggata
     8341 tcggtggggg aaaaacgcaa tgttactctg acggcgcagg cgttgacaat aaatgatgcg
     8401 gcggtttttg attctgtcca cggatgttgc cgacggcatt ttttgcgctt atttgaaatc
     8461 cttttccacg ctcatgaaaa tctgcgtgtt tttgcgtgtg taaaaacttt tcatattgct
     8521 gtcgattttc agatagcgga aattgagttg cggcgtaaag cccttccaag agattttgtc
     8581 gtgccaaaac gacaggtttg cctgatattc gtggtctttg cgcgggaagc ggtacacaat
     8641 ggtcccgggc gcgtcaaacg tcctgcgtgt atagcgcagg tttgcccgca gacccaagcc
     8701 gccgtcgaac gttttgaccg cgccgatacg caaacccttg cggacggaag cctgttccgc
     8761 ctccttcgtc aagtcgtgcg accagtccgc cccgccgtaa agcagccagt ctttcggcgc
     8821 ggaatacatc agcgtcgcgc ccgccagcgg catatggctg tcgtatcggg cggcggtgcg
     8881 gtcttcctga taatgcttcc acatattgcc cgcgtttagt gtcaaccgcc agcgttcgct
     8941 caagtgttgg gagaaatcgg cattaaagcc gccgacgaaa ttgtatcggc tgccgcctaa
     9001 gaggttttgc ccgacaaacg gcacgatgcc gaacgagcgc gttaccgaac gatttttata
     9061 gccgagcgac aggtgcaggc actgttcgct gaaatctttg ttatcccaat aatgcacgcc
     9121 gccgccgctg atgccgccgt agaggaaatg atgcccgccc gcattgattt cgcgcgacac
     9181 gcctaagccc tagcgcaaac cgtgtgcctt ttgcggcagg ctgtcggcgg ttttcgtcca
     9241 gcttctgccc gcaaattcaa tcgttttttt ggacgaagcg ttgtttatag cggattaaca
     9301 aaaatcagga caaggcgacg aagcctcaga cagtacagat agtacggaac cgattcactc
     9361 ggtgcttcag caccttagag aatcgttctc ttcgagctaa ggcgagacaa cgccgtac
//
LOCUS       DAWEWP010000070         9328 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWEWP010000070 DAWEWP010000000
VERSION     DAWEWP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.070"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1067..1735)
                     /locus_tag="WGN53_001208"
     CDS             complement(1067..1735)
                     /locus_tag="WGN53_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0140203.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEEEAKEKGWPV
                     LNFK"
     gene            complement(1732..2400)
                     /gene="hda"
                     /locus_tag="WGN53_001209"
     CDS             complement(1732..2400)
                     /gene="hda"
                     /locus_tag="WGN53_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ0140204.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2505..3122)
                     /locus_tag="WGN53_001210"
     CDS             complement(2505..3122)
                     /locus_tag="WGN53_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0140205.1"
                     /translation="MVWPEYSPQENVALFRHNGEILLSGINIKLKSGDSLLIRGPSGC
                     GKTSLLCALAGLWPFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPE
                     LAEAMNTCRLGYLIDKLDKTADWQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALD
                     EPAEAALYRALKQKLPDSIIISIGHRSTLNTFHNMHLNVGNVACD"
     gene            complement(3477..4376)
                     /locus_tag="WGN53_001211"
     CDS             complement(3477..4376)
                     /locus_tag="WGN53_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ0140206.1"
                     /translation="MPALTVTWMIYTKYLVYQIYLVIFCGVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            4420..4671
                     /locus_tag="WGN53_001212"
     CDS             4420..4671
                     /locus_tag="WGN53_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0140207.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            4673..6652
                     /locus_tag="WGN53_001213"
     CDS             4673..6652
                     /locus_tag="WGN53_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0140208.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DARCAVVAAVLGIKYQYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRSAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPGWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAPDMLSA
                     QELATMFMPQEVRKVQRGWLDLFNNSYFSVELAEYHKDEVRVSYDLSDASAVNVFDMD
                     GKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQAAVW
                     DELGHLGGNVIEAEYAVLPKTGTDDGIFLFEADRS"
     gene            6688..7602
                     /locus_tag="WGN53_001214"
     CDS             6688..7602
                     /locus_tag="WGN53_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0140209.1"
                     /translation="MKQINQALQQKLAEFKEKSGMSQTQLARGIGTSPASVSMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKAKREELVLGFVSTKTTRRIVEAMRNAHEGGEIS
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGHLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKYG
                     ELVQLHSRVAGALNLGDTMPDAELEQIARATLPEADDETIAELVKHSNGNTRRMSKLM
                     RGSVRVANKNGIRMQAGIVKKYSTLIIR"
     gene            7692..7958
                     /locus_tag="WGN53_001215"
     CDS             7692..7958
                     /locus_tag="WGN53_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140210.1"
                     /translation="MQVLKKVDWKMFVARSFWQCVPVGLTAGVWCFVAGMALHSCAKE
                     PEPVAKEPTKVEAMERQADLEVKVWEGQYESMSVEEKMKGIVYE"
     gene            8067..8240
                     /locus_tag="WGN53_001216"
     CDS             8067..8240
                     /locus_tag="WGN53_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140211.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            8233..8400
                     /locus_tag="WGN53_001217"
     CDS             8233..8400
                     /locus_tag="WGN53_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140212.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            8464..8691
                     /locus_tag="WGN53_001218"
     CDS             8464..8691
                     /locus_tag="WGN53_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140213.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            8706..9224
                     /locus_tag="WGN53_001219"
     CDS             8706..9224
                     /locus_tag="WGN53_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ0140214.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDTILNQKECFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
BASE COUNT         2483 a         1855 c         2611 g         2379 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tatacgtcct agattcccac tttcgtggga
       61 atgacgggat gtgggttttt gtgcggattt gaaccggtaa gggtggtgtg ggattggtgg
      121 tttgcttagg atcttttgga ttgtattttg tatatacatt tacttgttga taaaagataa
      181 aataaaatta gaaactaaaa gtgagaaaaa attaataata atagggatgt ataaatgtaa
      241 agactccgtt tcatagctaa ggttatctga atatatggaa aaaaagtaaa agtccataaa
      301 actaaaatat atagataatg ctaatgataa tagaattatc actttcctag agtaaggata
      361 atattttttt atcgtaaaaa aatataaaat aggataaata attgccatat agatagattt
      421 tgtaacaaaa ttaaaaataa ataataaaaa taatgtaata aagtatattt tttggctatg
      481 aaataaaatt gtacataatt gaacgagcag atcaaaaaat gaactacata taacaataaa
      541 taataacgta tttaccatac taaatttaat aggtctcatt atcatattta ataaccactt
      601 catagtatag tggattaaat ttaaaccagt acagcgttgc ctcgccttgc cgtactatct
      661 gtactgtctg cggctttgtc gccttgtcct gatttttgtt aatccactat aaatgcagag
      721 tgggtggaaa cactcacttt atggtttgct acgctctgct caattagcaa cccgataacc
      781 caatatggat aatagggtaa ttaatccaat ctaatttgtc agcatctgtt aatttattgc
      841 aaaataaagt attgaattat gtcgggtgca aatgacgaaa tataagtttc cgtgcggacg
      901 gatcaagatt cccactttcg tgggaatgac ggtggaaaga ttgttgtttt tcccgatgaa
      961 ttcctgtgtt ttttgttttt ccggataaat tcctgtggct ttgagttttt tggatttcag
     1021 cctcaatgcc gtctgaacgc cgaattgggc ttcagacggc attgcgtcat ttgaaattta
     1081 aaaccggcca gcctttttct ttggcttctt cttccagctc ggcatcgggg ttgacggcga
     1141 cgggttcgtc gacgaggcgc agcagcggca ggtcgttttt ggagtcgctg taaaaatagg
     1201 ttttgccgta gctttcgagc gtttcgccgc gttcggcgag ccattggttc aggcgggtga
     1261 ttttgccttc tttgaggctg ggcgtgccga tgtaattgcc ggtgtagcgg ccgtcggaac
     1321 cggattcgag ttgtgtgccg atgatgttgg taatgccgaa aaggtggcag acgggggtga
     1381 tgatgaactc gttggttgag gaaatcacaa gggtttcgtc gcctgccatt tggtggctct
     1441 gcactagcat acgctgcata ggcgagatgt gggggatgat gtattccgcc ataaattcgc
     1501 ggtgaaattc cgccagctct tctttgctgt aacgggcgag cggggcgagg tggaatttga
     1561 ggaatgcgtc gatgtcgagg cagccgtttt ggtagtcgcg gtagaatttt tcgttttgcg
     1621 cttcggtttc ggcagcgtca accaagcctt ttttgatgag gtattgcggc caggcgtggt
     1681 cggaatcggt gttgatgagg gtgttgtcga ggtcgaagat ggcgaggttt ttcattgggt
     1741 ttcctgttgt ttcaaaagct ggcgcaaaag cggcagggtg atgcgtttgc ccatcgtgac
     1801 ggcgtagttg tccagcgtgt cgagcatcat catcaggctg tccatatcgc gccgccagtg
     1861 tttgagcagg tattcgaaaa tttcggaatc aacggttact tggcgtgccg ccgccatact
     1921 ggcgagcgcg tcgatttttt cttggtcggt caggggtttg acttcgtaaa cgaggcagta
     1981 agccatacgc gtccgcaaat cttcgcggat gacgagctgc tggggcgtgt attccgaacc
     2041 gagcagcaaa aagcctttgc cgctgttgcg gaagcggttg aagatggaaa aaagcagggc
     2101 ttgttcttcg ttgcccagct tttcgacttg atcgacggcg aggtattccg cctcgaacgc
     2161 ggcatcggtc agcggcatgg aggcggcatc gatataggcg gcgtttttgc cggcttcgag
     2221 cgcctgtgcg acccacgcct gcaaaagatg gcttttgccc gcgccttctt cgccccaaac
     2281 ataaatgaat tgtccgtgtt tgtgtcggag gacatagacc agttccgcgt tttccgtgcc
     2341 gaggaatttg tcgaaactcg gatagtcgtg tgcggcaaag tcgaaaataa gctggttcac
     2401 ggttcggcat tccgaggggt ggtaaacggg tttattgtac gttgttttcg cgcgcctttc
     2461 caatttgaac gatgccgtct gaaaacggct tcagacggca tcgttcagtc gcaggcaacg
     2521 ttgccgacat tgaggtgcat attgtggaac gtgttgagcg tgcttcggtg tccgatgctg
     2581 atgatgatgc tgtcgggcag tttttgcttt aaggcgcggt agagtgcggc ttcggccggt
     2641 tcgtccaagg cggcggtggc ttcgtcgagc aggacgattt tgggcttgga aagcagggcg
     2701 cggacgaagg cgacgcgttg cagttcgcca ggggagagtt tgtgctgcca gtcggctgtt
     2761 ttgtccaatt tgtcaatcag ataacccaag cggcaggtgt tcatggcttc ggctaactcg
     2821 ggatgctgtt tgtcgatgtc cggataacaa accgcgtcgc gtaggctgcc ttgcgccgtg
     2881 tacgggcgtt gcggcaggaa gaggatgtct tgatgcggcg gacggctgac tttgccgctg
     2941 ctgccgaacg gccaaagccc agccagcgcg cacagcaacg aagttttgcc gcaaccgctc
     3001 gggccgcgta tcagcaggga atcgccgctt ttgagtttga tgttgatgcc gctcaacagg
     3061 atttcgccgt tgtggcggaa cagagcgacg ttttcctgtg gggaatactc cggccatacc
     3121 actcaacacg gcctatgatg gcgatgtcgt cttgggtatt actcaaatct atttcaaacg
     3181 gtgcgtaacg tggattttca gacgttacaa gcagtttgcc cggtatacgt tgcacacgtt
     3241 tgacaaagag gtcattgcct atacgcaaga catataggcc gtcgcgcggc tcggtttcgg
     3301 cgtggttgat tagaatgttt ataaaaggct ttaactagaa actgaacatg gaacttgtat
     3361 aatagttcca tgtttgaaaa aatcttttaa tatcatacat ctaaaaaaac aaagcatctg
     3421 aagaccataa gctaaacatg aaacttgaat ttaaaggcgg agtttcatgt ttaagcctat
     3481 tttttccgtt ttttagtcga tactgctgca tatgtttttt gcaaatcttc atcacttaat
     3541 tcagtcaggc tttctactga gaaatttttt tcgaggtaac ttttcaacca atcttcaagc
     3601 tcaagctgtt ttacatttaa tttgatataa gcgtatttct tttttcgcca gtcgggatcg
     3661 ttgttctttg atgttgctgt attgctcaac ctacccagcc attttctgag ataagtttct
     3721 gctttgtcgt aatcgctcaa tgcgattagt ttgtaacttg gaactttaca gtgagcattc
     3781 agactagccc aaattgcacg gattgatttt ggcgaccgtt tggcaatctc ttctgctgcg
     3841 gcaacttgtt taacaagttg ttgcagcttt gaagcttgct caagcgttat atgttccttg
     3901 ccgggttttg aatcgacacg ggttctgatg acgtgatttt gggtattaat ttgatgaaca
     3961 accgaaccag atgaggcgat aacagcgtta ctgacgttgc cgttaatctg attctcaacg
     4021 tttgatttgc caatagcgtc gtccaatttt ttattaaggt cgtctgaaac agcatttaaa
     4081 tttgccgaat gaatgcctgt aaagacaaat tgaatatcca cccctaattg gacagcagca
     4141 agtaaaaagt ccgcaggaat gttgacctgc ccgctttcat acagtctcaa actttctgca
     4201 ctacatccag tttgctcagc aaaattcttt cgagagtatc caagtcggct tctctcttca
     4261 actaaattca atgctaaatc aatgcgttcc ataaaaacac cgcaaaatat taccaaatat
     4321 atttggtata ccaaatattt tgtgtatatc atccaagtaa cagttaaggc aggcattaaa
     4381 gcctgtaagg taagtaatgg attctatcat ggaggcatta tgcaagcgaa cgaaatctat
     4441 aaatccttag aagaaaaaaa tatatcggca agaatgttgg cttcggcttt aggggtaact
     4501 aaccaatctg tttctgaggt cataagaaat ggacgaggaa gcaaaagaat tgcggaggca
     4561 atcgcgaaag tgattcaaaa agatttagtt gaagtttttc cgcattacaa gaaaacagat
     4621 tgtcgagaca aaaagatagc tgaattgaaa gagatgctcg gagtgggttg aaatggaata
     4681 tatggatctc aaaagcctgc ttaattttgg gttgccaaat atgccaagaa cagtaatagg
     4741 gctgagaaaa aaagcgcaaa gggatggttg gcaaacgaga aggcgtcaag gtaagggtgg
     4801 tggtgttgaa tatgccctcc ctgccgaaat ccgagcagcc atcatgaaac ggcagtcgga
     4861 cgagctggcg gagaagatgc cgaaaaccct gccccaagtc agaccgggga cggcgatgtc
     4921 ggctcaggca ctggctgaag cggccaagct gttgaacgag aaacaacggt cggtggcgga
     4981 tgcgcgatgt gcggtggtgg cggcggtgtt ggggattaaa taccaatacg attgctctgc
     5041 caaggctgcg gtggctcagt ttttgggctt gctggcagaa ggtaaattgg acgcggtcac
     5101 gcttgggaac ttggaaaagg ccaatgaccg cagccggtcg gcgaaggttg gcgaacgtac
     5161 tttagacggc tggatttctg cttatttgaa agcggaaaac gcgacggagc ggttggttgc
     5221 tttggctccg aagacgacga aggcggtcaa gccgattgaa agttacggat ggttgccgat
     5281 gtttatgcag tttcacaata ttccgtccgc gccaaagctg gcgcacagct accgccggtt
     5341 tgtgcagtgg gcggaagcgg aaaatatgcc ggtcaatgat gtgcctaact tgagtatggt
     5401 gcggcgcgtt tgggacaagc tgccgctgat tatgcaggag cgcggcagga aaacgggggc
     5461 ggcttataaa tcgctgctgc cttatgtgaa acgtgattgg ggggctttga agccgaacga
     5521 tgtttggatc ggcgacggcc acagctttaa ggcgaaggtg gctcatccgg tacacggcag
     5581 gccgtttaag ccggaagtga cggtgattat tgatggttgt acgcggtttg tggtgggttt
     5641 ttcggtgtcg ttggctgaaa gttgtgtggc ggtatcggac gctctgcgta tcggggtcaa
     5701 gcactttggt ttgccgatta tctactactc ggataacggt ggcggccaaa ccggcaagac
     5761 gatagaccat gaaatcacgg gtattacgtc ccgattgggt atccggcatg aaacgggtat
     5821 cgcgggcaac ccgcaaggcc gaggcatcat cgagcgatgg tggaaagaca atctgattga
     5881 gatggcgcgc cagtatgaga cgtttgcggg cgcggggatg gacagcagta ccaagaacct
     5941 gatgtaccgc aagatggaaa gtgcgtttaa cgctttggaa aaaggcaagg atttgacgga
     6001 ggaacaacag aaatatttga aaaaactgcc gggctggtcg cgttttatcg cggatgtggt
     6061 caagtgtatc gacgaataca acaaccgccc gcacggcgag ctgccccgac atccggacgg
     6121 cgggcattat tcgccgaagg cttatcggga aatgaggctg gaacaggacg gtatcgcgcc
     6181 ggatatgttg tcggcgcaag agctggcgac gatgtttatg ccgcaagagg tgcgaaaggt
     6241 acagcgcggt tggctggatt tgttcaacaa ctcttatttt tcggtggagc tggcggagta
     6301 tcacaaagac gaggtacggg tcagctacga tttgagcgat gcgtcggcgg tcaatgtgtt
     6361 tgatatggac ggcaagttta tcactaaggc gcaggccaac ggcaataccc gcgaggcttt
     6421 cccgacggct cgtatcgacc aactggcgga aaaacgccga aaaggcaaaa tcaagcgggc
     6481 ggaaaatgca atcaagctcg caaatgcaga agtcaatcct gctttggaac aggctgcggt
     6541 ttgggacgag ctgggacatt tgggcggaaa cgtcatcgag gcggagtatg cggtattgcc
     6601 gaaaacgggc acggacgacg ggattttctt gtttgaggcg gatagaagtt aaaacggttt
     6661 taaacctctt ttaaaaggac taaaaaaatg aaacaaatca atcaagcatt gcaacaaaaa
     6721 ctggctgaat ttaaagaaaa atcgggtatg agccaaaccc aactggcacg cggtatcggt
     6781 acttcgccgg catccgtcag tatgtatctg aacggtactt atgcggaaaa aggcggcaat
     6841 tatgaaacca tcgagccaaa aatcgaggcg tttttggaga tgcaggacag caaagcgaaa
     6901 cgcgaagagc tggtgttggg ttttgtatcg actaagacga cacgccgaat cgtggaagcg
     6961 atgcgcaatg cgcacgaagg cggcgagatt tccgttatct acggtcaggc gggtttgggc
     7021 aagacgcagg cggtcaaaaa ctactgcgag aaaaaccctg cagccatctt gattgaggct
     7081 aatccgagct ttacggcttt ggtcttgatg cgcaagttgg cgacggcggc gaaggtatcg
     7141 gcgatgggca gcttgaatga tttgtttgaa tcggtatcgg acagattgcg tgattcaggc
     7201 catctgattg tggtcgatga agcggaaaac ctgccattac gcgcccttga gattatccgc
     7261 cgactgcacg atgagactgg ctgcggtttg gtgttgagcg gtatgccccg actggtggcg
     7321 aatttgcgcg gtaagtatgg cgagttggta cagcttcaca gtcgtgtggc gggtgcgctg
     7381 aatctaggtg atacgatgcc cgacgcagag ttggaacaga ttgcgcgggc aaccctaccc
     7441 gaagccgatg atgaaacgat tgcggaactg gttaagcata gcaatggtaa tacacggcgt
     7501 atgagcaagt tgatgcgcgg atcagtacgg gtagcgaata aaaacggtat tcgaatgcaa
     7561 gcgggaatcg tgaaaaaata ttcgacattg attatccgat aggccgtctg aaacggtaag
     7621 tctctgacag ggctatatat tttttaccct atgattttaa taagttattg ttttctaaag
     7681 gaaaactcaa aatgcaagtt ttgaagaaag ttgattggaa gatgtttgtg gcacgctctt
     7741 tttggcagtg tgtgccggtt ggtttgacgg cgggcgtgtg gtgttttgtg gcgggaatgg
     7801 cgttgcattc ctgcgcgaaa gaacccgaac cggttgcgaa agagccgacg aaggttgagg
     7861 cgatggaaag acaggcggat ttggaggtca aggtatggga agggcaatac gagtcaatga
     7921 gcgtcgagga aaaaatgaaa gggattgttt atgagtgata agccaatttt attgagccct
     7981 gcggcgaaaa aagaggcttt ggcacgggcg gtaaaggaaa tccgcgcgaa atatggcgat
     8041 aaggcgattg tgaaaggatg tgtgaaatga gtttcggacg acgtaatacg gattggcagg
     8101 cttggggaca acaccgcagg cgtgcgacgg cgcgaatggc gcaaaaaagc cgggagcgcg
     8161 aaatcgagga atatcaggcg cagtttgaag ataaggacgg caaaggtcgt ctgaaaacgg
     8221 aaggaaacaa agatgaatga aaaagattta atcgaatggc tcgaagaccg tggcgagctg
     8281 atggtcatga agaaggacgg cgaaggcttt gtgattgcag cgcgtgcgcc ggacgggatg
     8341 tggaagactg ccgaggcgga aactttggct caggcgataa ctttatggga ggaagtgtga
     8401 tgtttaagtt tttgagaaac cgcgcgaaag ttgaagcaat tttaaacaag aaaggaaagt
     8461 gaaatgaaag tgtatgtttt taaaattagt aatgaaaacg gtaagttaaa gatagaactc
     8521 cccgaaatcc caatgggtaa gcaaattgac gaagttgatt tgattgctgg gctaaccacg
     8581 gaatttattg caagtatgtt acgtgatgcc caaaaagatc gtcgcaaatt cgtaatcgac
     8641 gcatcaaatc aattggctgc aatccagaca tatcaaaaaa tcttcaatta aggaaggaag
     8701 agaaaatggc taaaacccga atcaaacagc ccgccatcga agcggcacaa gacaaaacgg
     8761 aagttactgc atttatccgc caaatcggcg acttgcagcg cgaagtcaaa cgcctggaaa
     8821 ccgaagccgg agacaaaaaa gcggctatcg aagaagaata tgccgccaaa gccgcgccga
     8881 tgtgtgccga aatcatgagc ctgaccgaac gtgtggccgc ctactgcgaa gcgcataagg
     8941 acgagctgac ggaaaatggt aaaaccaaaa ccgtggactt taccaccggc ctgattaaat
     9001 ggcgcatccg tccgccatct gtcaaggtaa cgggcgtggc cgccgtcctg gcgtggctct
     9061 cggaaaaatc cgcctttgcc gagtttgtcc gcactaaaaa ggaaatcgac aaagacacca
     9121 tcctgaatca aaaagagtgt ttttcagacg gccaagttcc ggggattaag attgtgagcg
     9181 gcgtggagga ttttgtgatt gagccgagcg aacaaattct ataaaatcaa cagcctgatt
     9241 tcaattcagt gtgttgaaac gtgattgagc ctacggagca ggagcttgcc tagccggttc
     9301 aaataggctt taacccatga tttaaagg
//
LOCUS       DAWEWP010000071         9325 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWEWP010000071 DAWEWP010000000
VERSION     DAWEWP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.071"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            168..1511
                     /locus_tag="WGN53_001220"
     CDS             168..1511
                     /locus_tag="WGN53_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ0140215.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     DRYAPDALAKLSGKEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTGRVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LLGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1514..2746
                     /locus_tag="WGN53_001221"
     CDS             1514..2746
                     /locus_tag="WGN53_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ0140216.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFAVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2739..3515
                     /locus_tag="WGN53_001222"
     CDS             2739..3515
                     /locus_tag="WGN53_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ0140217.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3515..4141
                     /locus_tag="WGN53_001223"
     CDS             3515..4141
                     /locus_tag="WGN53_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ0140218.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4145..4738
                     /gene="nqrE"
                     /locus_tag="WGN53_001224"
     CDS             4145..4738
                     /gene="nqrE"
                     /locus_tag="WGN53_001224"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ0140219.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4752..5969
                     /gene="nqrF"
                     /locus_tag="WGN53_001225"
     CDS             4752..5969
                     /gene="nqrF"
                     /locus_tag="WGN53_001225"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ0140220.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6121..7176
                     /locus_tag="WGN53_001226"
     CDS             6121..7176
                     /locus_tag="WGN53_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0140221.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     TGGYRTAMSSEFEKLLR"
     gene            7202..7417
                     /gene="nqrM"
                     /locus_tag="WGN53_001227"
     CDS             7202..7417
                     /gene="nqrM"
                     /locus_tag="WGN53_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ0140222.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7541..8119)
                     /gene="grpE"
                     /locus_tag="WGN53_001228"
     CDS             complement(7541..8119)
                     /gene="grpE"
                     /locus_tag="WGN53_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ0140223.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8303..9121
                     /gene="cysE"
                     /locus_tag="WGN53_001229"
     CDS             8303..9121
                     /gene="cysE"
                     /locus_tag="WGN53_001229"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ0140224.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2252 a         2624 c         2314 g         2135 t
ORIGIN      
        1 taaaaacggc ttccgcaagg aaaaaagcag caaatctgac aaacaggtac aattcctgat
       61 agaatctaac actttgctac attaatttat tcaatgcttt tgatgccacg cgacacggta
      121 tcaaaacacg ccgtttttac cttaaccatc acaagtaatg caaatatatg attaaaatca
      181 aaaaaggtct aaacctgccc atcgcgggca gaccggagca agtcatttat gacggcccgg
      241 ccattaccga agtcgcgttg cttggcgaag aatatgccgg tatgcgcccc tcgatgaaag
      301 tcaaggaagg cgatgccgtc aaaaaaggcc aagtgctgtt tgaagacaaa aagaatccgg
      361 gcgtggtgtt taccgcgccg gcttcaggca aaatcgccgc catccatcgc ggcgaaaagc
      421 gcgtacttca gtcggtcgtg attgccgttg aaggcaacga cgaaatcgaa ttcgaccgct
      481 acgcgcccga tgccttggca aaattgagcg gcaaagaagt gcgccgcaat ctgattcaat
      541 ccggtttgtg gactgcgctg cgtacccgtc cgttcagcaa aatccctgcc gtcgatgccg
      601 agccgttcgc catcttcgtc aatgcgatgg acaccaatcc gctggcggca gaccctgtgg
      661 ttgtgatcaa agaagccgcc gaggatttca gacgaggttt gctggtattg agccgtttga
      721 ccgagcgtaa aatccatgtg tgtaaagcag caggcgcaga cgtgccgtct gaaaacgccg
      781 ccaacatcga aacacatgaa ttcggcggcc cgcatccggc cggtttgagt ggcacgcaca
      841 ttcatttcat tgagccggtc ggtgcaaaca aaaccgtttg gaccatcaat tatcaagatg
      901 taattgccat cggacgtttg tttgcaacag gccgtctgaa caccgggcgc gtggttgcct
      961 tgggtggttc tcaagtcaac aaaccacgcc tcttgcgtac cgttttgggt gcgaaagtat
     1021 cgcaaattac tgcgggcgaa ttggttgacg cagacaaccg cgtgatttcc ggttcggtat
     1081 tgaacggcgc gattgcacaa ggcgcgcacg attatttggg acgctaccac aatcagattt
     1141 ccgttatcga agaaggccgc agcaaagagc tgctcggctg ggttgcgccg cagccggaca
     1201 aatactccat cacgcgtaca accctcggcc atttcctgaa aaacaaactc ttcaagttca
     1261 acacagccgt caacggcggc gaccgcgcca tggtgccgat tggtacttac gagcgcgtga
     1321 tgcccttgga tatcctgccc accctgcttt tgcgcgattt aatcgtcggc gataccgaca
     1381 gcgcgcaggc attgggttgc ttggaattgg acgaagaaga cctcgctttg tgcagcttcg
     1441 tctgcccggg caaatacgaa tacggcccgc tgttgcgcaa agtgctggaa accattgaga
     1501 aggaaggctg attatgggct tgaaacattt tctggaaaaa atcgaaccgc acttcctgcc
     1561 gggcggcaaa catgaaaaat ggtatgccct ctacgaagcg gcggcgacga ttttctacac
     1621 atccggtgcg gtaacgcgca aagcggcaca cgtccgcgac gcgctcgact ccaagcgcat
     1681 gatgattttg gtgtggctgg ctttgttccc cgccatgttt tacggcatgt acaacgtcgg
     1741 cgcacaggca ttcggtgcct taacgcccga tttgctgcaa caaagcatcg ccaacgactg
     1801 gcattacacc cttgccgacg ctttgggcat caatatgtcg tctgaagcgg gcgtgttggg
     1861 caaaatgctg ttcggcgcga tttacttcct gccgatttac gcgaccgtat ttgccgtcgg
     1921 cggtttctgg gaagtcttgt tcgccaccgt acgcaaacac gaaatcaacg aaggtttctt
     1981 tgttacctcg attctgtttg ccttaatcgt tccgcccacg ctgccgctgt ggcaggcggc
     2041 tttgggtatt tctttcggcg ttgtggttgc gaaagaagta ttcggcggta cgggtaaaaa
     2101 ctttatgaac cctgcgctgg caggccgcgc cttcctgttc ttcgcctacc ccgccaactt
     2161 gagcggcgat gcggtttgga cggcggttga cggctattcc ggcgcaaccg cgctggcgca
     2221 atgggcggca cacggtgcag acggcctgaa aaacgccgta accggtcaaa ccatcacttg
     2281 gatggacgcg tttatcggca aactgcccgg ctccatcggc gaagtctcca ctttggcact
     2341 cttaatcggc ggcgcgttta tcgtgtttgc ccgcatcgct tcttggcgca ttattgccgg
     2401 cgtgatgatc ggtatgattg cgatgtcttc gctgttcaac ttcatcggtt cggacaccaa
     2461 cgctatgttt gccatgcctt ggtactggca tttggtcgtc ggcggcttcg ccatcggtat
     2521 gctgtttatg gcgaccgacc ctgtttccgc ttcctttacc aatgtcggca aatggtggta
     2581 cggcgcgctg atcggcgtga tgtgcgtgtt gatccgcgtg gtcaatccgg cttaccccga
     2641 aggtatgatg ttggcgattc tgtttgccaa cctgtttgcc ccgattttcg actatttcgt
     2701 cgcacaagcg aacatcaaac gcagaaaggc gcgcagcaat ggctaagaaa ttcgataaag
     2761 acagcttcag cggcacgctg attgtcgtgt tggcggtcag cctgatttgc tcggtcatcg
     2821 ttgccggtgc ggtcgtcggc ttgaaaccca tccaagagaa acaaaaactc caagacaaac
     2881 aaggctatat cttgagcgta gccggtttga tggataagga caccgacatc ggcaaaacct
     2941 ttgccgagcg tatcgagcaa cgtgttgtcg atttggcgac cggcgaatac gtcgccgatg
     3001 cgcctaaaga cttcagcgcg cgcatcgcag gcaaagaccc tgcccaaagc atccgcatca
     3061 aacccgaaga cgatttggca ggcatcaaaa gccgtgccaa atacaccgag gtttatttgg
     3121 taaaaggcga agacggcaaa atcgggcaaa tcatcctgcc tatgcacggt aacggtttgt
     3181 ggtcggtcat gtacggcttt gtcgccatcc agcccgacgg caacaccatc aacggcatta
     3241 cctactacga acaaggcgaa accccgggct tgggcggcga aatcggcaat ccgttgtggc
     3301 agcaaaaatt cgtcggcaaa aaactgtttg acggacaagg caaactcgcc ctgcacgtcg
     3361 gcaaaggcgc gggttcggac aaagaacacg gcgtagatgc cctctccggc gcatcgctga
     3421 catccaaagg cgtgcaaggt tcgttcgcct actggttcgg cgaaaacggc tatatcccct
     3481 acctgaacaa attgaaatca gcaggagcgc aataatggct gatatgaaac gcttgaaaca
     3541 tttgatgttt tcacccttta tcgacaacaa cccgattgcc ttgcaggttt tgggtatttg
     3601 ttcggctttg gcggttacca ccaaacttca gacggccatc gtgatgggta tttccgtagc
     3661 tttggtaacc ggtttttcca gcttcttcat ttcgctggta cgcaactaca tccccaacag
     3721 catccgtatt atcgtgcaaa tggcgattat cgcgtcgctg gttacgctgg tcgaccaact
     3781 cttgcaggca tttgcctacg aattgtccaa acagctttcc gtattcgtcg gtctgattat
     3841 taccaactgt atcgtgatgg gccgcgccga agcatttgcg atgaaagagc cgccgctgga
     3901 aagcctgatc gacggcatcg gcaacggcgc gggctacggg ataatgctgc ttgtcgtcgc
     3961 caccgtccgc gaactgattg gctcgggcaa actcttgggc tacaccgttt tccaaaccgt
     4021 gcaggacggc ggctggtatc agaccaacgg cttgttcctg ctcgccccca gcgcgttctt
     4081 catcatcggc tttttgattt ggggactgcg tacctggaaa cccgaacagg cggaggaata
     4141 agctatggaa cactatttga gcctcttcat caaatccgtc ttcattgaaa atatggcact
     4201 atccttcttt ttgggtatgt gcacgttttt ggcggtatcc aaaaaagtat ccaccgcatt
     4261 cggtttgggt gtggcggtaa ttttcgtact cgggctgtcc gtccctgtca accaactcgt
     4321 ttactcgctg ctcaaagacg gcgcgattgt cgaaggcgtg gatttgacct ttttgaaatt
     4381 catcaccttc atcggcgtga ttgccgcttt ggtgcagatt ttggaaatgt tcttggataa
     4441 attcgtcccc gccctctata acgcattggg catctacctg ccgctgatta ccgtgaactg
     4501 cgcgattttc ggcgccgtat cgtttatggc gcaacgcgaa tacaacttcg gcgagtccgt
     4561 tgtatacggc ttcggcgcgg gcttgggctg gatgttggcg attgtcgctt tggcgggcat
     4621 taccgaaaaa atgaaatatt cggacgctcc caaaggcctc aaaggcttgg gcattacctt
     4681 tatcgccgcc ggcctgatgg cgatggcgtt tatgtcgttc tctggcatcc agttataaga
     4741 aagggatcgg tatggagatt attttaggta tcgtgatgtt taccgtcatc gttttggttt
     4801 tggcactgat gattctgttt gccaaatcca aactggtcag cgaaggcgac atcaccatca
     4861 aagtcaacgg cgaaaaagag ctgacgatgc ccgccggcgg caaactcttg ggcgcgcttg
     4921 ccaacgaagg catctttatc ccctccgcct gcggcggggg cggttcgtgc ggacaatgcc
     4981 gcgtcgtcgt aaaaagcggc ggcggcgaca tcctgccgac cgagctgtcc cacatcagca
     5041 aacgcgaagc acgcgaaggc tgccgtctgt cgtgccaagt caacgtcaaa accgacatgg
     5101 acatcgaagt ccccgaagaa gtgttcggcg tgaaaaaatg ggaatgcacc gtcatctcca
     5161 acgataacaa agccacgttc attaaagaac tcaagcttgc cattcccgaa ggcgaagaag
     5221 tccccttccg cgccggcggc tacattcaaa ttgaagcccc gccgcacacc gttgcctaca
     5281 aagacttcga cattcccgag gaataccacg aagactggga caaatacaat ttgtggcaat
     5341 acgtttccaa agtggacgag ccgattttgc gtgcctactc tatggcttcg tatcctgaag
     5401 aaaaaggcat cattatgctg aacgtgcgta tcgccacgcc gcccccgcgc gtacctgatg
     5461 cgcctccggg acaaatgtcg tcctacatct ggtcgctcaa acccggcgac aaagttacga
     5521 tttccggccc gttcggcgaa tttttcgcca aagacaccga tgccgaaatg gtatttatcg
     5581 gcggcggtgc gggtatggct ccgatgcgtt cccacatttt cgaccagttg aaacgtttga
     5641 actccaaacg taagattacc ttctggtacg gcgcacgttc caaacgcgaa atgttctatg
     5701 tcgaagactt cgaccaactc gcagcagaat tcccgaactt cacatggcac gtcgccctgt
     5761 ccgacccatt gcctgaagac aactgggacg gctacacagg cttcattcac aacgtggttt
     5821 acgaaaacca tctgaaaaac cacgaagcgc cggaagactg cgaattttat atgtgtggtc
     5881 ctccgatcat gaaccagtcc gtcatcaaaa tgcttaaaga cttaggcgtg gaagacgaaa
     5941 acatcctctt ggacgatttc ggcggttaaa tattcggctt caaacacaaa aggaagaaac
     6001 ctcggtttct tcctttttct atccgatgcc gcccgaaccc ccttcagacg gcatcggcat
     6061 agaaacatat cggccgtttt gcgatataat tcaaaccatt tgccaaaacc gtcaaacact
     6121 atgccgtctg aaacacgcct gccgaacttt atccgcgtct tgatatttgc cctgggtttc
     6181 atcttcctga acgcctgttc ggaacaaacc gcgcaaaccg ttaccctgca aggcgaaacg
     6241 atgggcacga cctataccgt caaatacctt tcaaataatc gggacaaact cccctcacct
     6301 gccgaaatac aaaagcgcat cgatgacgcg cttaaagaag tcaaccggca gatgtccacc
     6361 tatcagcccg actccgaaat cagccggttc aaccaacaca cagccggcaa gcccctccgc
     6421 atttcaagcg acttcgcaca cgttactgcc gaagccgtcc gcctgaaccg cctgacacac
     6481 ggcgcgctgg acgtaaccgt cggccccttg gtcaaccttt ggggattcgg ccccgacaaa
     6541 tccgttaccc gtgaaccgtc gccggaacaa atcaaacaag cagcatctta tacgggcata
     6601 gacaaaatca ttttgaaaca aggcaaagat tacgcttcct tgagcaaaac ccaccccaag
     6661 gcctatttgg atttatcttc gattgccaaa ggcttcggcg ttgataaagt tgcgggcgaa
     6721 ctggaaaaat acggcattca aaattatctg gtcgaaatcg gcggcgagtt gcacggtaaa
     6781 ggcaaaaacg cgcgcggcga accttggcgc atcggcatcg aacagcccaa catcgtccaa
     6841 ggcggcaata cgcagattat cgtcccgctg aacaaccgtt cgcttgccac ttccggcgat
     6901 taccgtattt tccacgtcga taaaagcggc aaacgcctct cccatatcat taatccgaac
     6961 aacaaacgac ccatcagcca caacctcgcc tccatcagcg tggtcgcaga cagtgcgatg
     7021 acggcggacg gcttgtccac aggattattc gtattgggcg aaaccgaagc cttaaagctg
     7081 gcagagcgcg aaaaactcgc tgttttcctg attgtcaggg atacaggcgg ctaccgcacc
     7141 gccatgtctt ccgaatttga aaaactgctc cgctaaacat acaaaccgat taggaaacac
     7201 catgaaaacc ctgctcctca ccttcggcat cttcctgacc gtcatcatcg gtatggcggt
     7261 cggctatatc ttctccaaac gcaccatcaa aggcagttgc ggcggcatta ccgccttggg
     7321 tatgaaaaaa atgtgcgact gcgacacgcc ttgcgacacc ctgcaaaaga aactggatga
     7381 agaaaaacag gcaggcggca tacgggttga ccgttaacac cgaaacaaaa tgccgtctga
     7441 agcccgacag gcgggttcag acggcattgc cgctttactt cgatcggttt atacgcaaaa
     7501 cgcgcttcag gaaataaatc agattattcc ccagacgcct tcaggcttcc ttctgtgcca
     7561 ccgtaaccat agccgggcgc aacacgcggt cggacagcgt ataacccttc ttcatcacgc
     7621 ccaccacggt attgggttcc tgttcgcttg ccaccgcctg catcgcctga tggatattcg
     7681 gatcgagctt atcgcccgct ttaggattga tttccttgat ttgcgtagca tcaaatgctt
     7741 tctgcaactc gttcaaagtc atctgcacgc ccattttcag cgcatcgaaa ttgccgctct
     7801 gatccaaaag cgccatttcc agataatcct tgaccggcag catttccacg gcaaacttct
     7861 gtccggcgaa cttgtgcgta tccgcaattt cctgctggtg gcggcggcgc aggttttgct
     7921 cgtttgccaa agcgcgcagc tgctcgtctt tcaactgcgc ttccagctcg gcaatccgcg
     7981 cctgcaaatc ctcataagcc ggctctgcgg cagcctgttc ctgcacaccg tccgcatttc
     8041 ctactgtctc gacggtttcc accgcctcca cattttcaac cgcttcttca ctgttttgct
     8101 gctgtgtctg ttcgctcata tcgtatccct taaaacaaat tggaaatcaa aatccagtta
     8161 ttacccgcgc aagataagga cattttcaag aaacttcaag caaaagcaga gaatttcaaa
     8221 ttcattttca aatcccctac cgaaaaaata atatagacgg taagatactg ccgaccatat
     8281 cgattttcaa acgtcatcac tcatgaaaaa agaccattta aacacaaccg gtttcgacct
     8341 ctggcacacc atccgcgaag aaaccgcggc cgcggtctcc gccgaaccga tgctggcaag
     8401 ttttttgcac caaaccgtgt tgcgccacga gtccctcggc tccgtccttg cctaccacct
     8461 ttccagcaaa ctcggcagcc cgattatgga cgtgcgcgcg ctgtttgaaa tctaccagca
     8521 ggcgttgggc agcgacaccc aaatcggcaa atgcgtcgaa gcagacttaa aagccatcta
     8581 cgaacgcgat cccgcctgcg acgaatattc gctgccgctt ttatatttca aaggcttcca
     8641 cgcgattcag gcacaccgca tcaaccatcg gctgtatctc gacgggcgca aaacgctggc
     8701 gtatttcctg caaaaccgta tgtccgaagt attcggcgtg gacatccatc ccgccgcccg
     8761 tttcggatac gggctgatgc tcgaccacgc caccggcttt gttgccggcg aaaccgccgt
     8821 gttgggcaac aatatttcga ttttgcacgg cgtaacgctc ggcggttcgg gcaaagaagg
     8881 cggcgaccgc caccctaaaa tcggcgacgg cgtgatgatc ggcgcaaacg cctcgatatt
     8941 gggcaatatc cgcatcggca gcaatgccaa aatcggggcg ggcagcgtcg tggtttcaga
     9001 cgtgccgccg tccatcacgg ttgtcggcgt acccgccaaa cccgtggcgc gatcgctcaa
     9061 aacaccgtcg gcggatatgg atcaaaatat ccagtttacc gaaatcgact ttatgatttg
     9121 aatatccgca ccaatgccgt ctgaaacgcc aaacgggctt cagacgacat tgcccgcttc
     9181 aagcctgcgc cttgatatgc cgccgatgtc ggcacttgcc agtcggacaa aatgcctttt
     9241 attcaataca cccgattccc acgcaaccgg aaatatagtg gattaacaaa aatcaggaca
     9301 aggcgacgaa gccgcagaca gtacg
//
LOCUS       DAWEWP010000072         9318 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWEWP010000072 DAWEWP010000000
VERSION     DAWEWP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.072"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..402)
                     /locus_tag="WGN53_001230"
     CDS             complement(<1..402)
                     /locus_tag="WGN53_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0140225.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTRE"
     gene            641..1540
                     /gene="crgA"
                     /locus_tag="WGN53_001231"
     CDS             641..1540
                     /gene="crgA"
                     /locus_tag="WGN53_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ0140226.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1767..4982)
                     /gene="carB"
                     /locus_tag="WGN53_001232"
     CDS             complement(1767..4982)
                     /gene="carB"
                     /locus_tag="WGN53_001232"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ0140227.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFKLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEQGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4989..5537)
                     /locus_tag="WGN53_001233"
     CDS             complement(4989..5537)
                     /locus_tag="WGN53_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140228.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(5540..6103)
                     /locus_tag="WGN53_001234"
     CDS             complement(5540..6103)
                     /locus_tag="WGN53_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0140229.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(6510..6677)
                     /locus_tag="WGN53_001235"
     CDS             complement(6510..6677)
                     /locus_tag="WGN53_001235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140230.1"
                     /translation="MADFDWIIENNRIKPTELSIYKGRLKNKKQPAQPVFPAEPLIPT
                     AAPSSLPVGKR"
     gene            complement(6681..7070)
                     /locus_tag="WGN53_001236"
     CDS             complement(6681..7070)
                     /locus_tag="WGN53_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ0140231.1"
                     /translation="MSTHEKLLTADNPVLHQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7662..7787)
                     /locus_tag="WGN53_001237"
                     /pseudo
     CDS             complement(7662..7787)
                     /locus_tag="WGN53_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7833..8966)
                     /gene="carA"
                     /locus_tag="WGN53_001238"
     CDS             complement(7833..8966)
                     /gene="carA"
                     /locus_tag="WGN53_001238"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ0140232.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2119 a         2343 c         2383 g         2473 t
ORIGIN      
        1 ttcgcgggta aacgcctcaa tcggcgcatt ccaagtcagt gaaagcatat gttttttgcc
       61 ttgcaacaag ccgcctgtgc cgtagccctc agtcggattg acgcggtgtc tgccgtcgct
      121 ttggtagagt ttgccgtgtc cagcggttaa tacttcgtct atgtattttt tcactgtcca
      181 aggctcgtgc atccaccagc ccggcatctg ccaaatcacg gcatccatcc aaacgaattt
      241 ttcgatttct gcctcaacat catagccggc atcaatcacg gtttcttgaa cattgtgtcc
      301 gagcgcggtc aaaacttctt tcgctttttt gtgaagcgtg cggtttaacc cgccgtgaga
      361 atgtccgaac gccttgccgc cgtctaataa taaaatattc atttgttacc tcgtttgtga
      421 attgatgaat gtattgtgcc gttttacttt ataatttaaa agtgcaaaaa taagaaaaca
      481 cttttgcgtc aaatgaaata atcagatgtt tcaacacacg ggatgacaca taaagcgtct
      541 ccctatgtgc cgtcctgatt cggaaggggt tacacccctc ccaaataaag tctgatcctg
      601 ccgccctaaa gggcggggtt ccaaccgaaa aggaaacacg atgaaaacca attcagaaga
      661 actgaccgta tttgttcaag tggtggaaag cggcagcttc agccgtgcgg cggagcagtt
      721 ggcgatggca aattctgccg taagccgcat cgtcaaacgg ctggaggaaa agttgggtgt
      781 gaacctgctc aaccgcacca cgcggcaact cagtctgacg gaagaaggcg cgcaatattt
      841 ccgccgcgcg cagagaatcc tgcaagaaat ggcagcggcg gaaaccgaaa tgctggcagt
      901 gcacgaaata ccgcaaggcg tgttgcgcgt ggattccgcg atgccgatgg tgctgcatct
      961 gctggcgccg ctggcaacaa aattcaacga acgctatccg catatccgac tttcgctcgt
     1021 ttcttccgaa ggctatatca atctgattga acgcaaagtc gatattgcct tacgggccgg
     1081 agaattggac gattccgggc tgcgtgcacg ccatctgttt gacagccgct tccgcgtaat
     1141 cgccagtcct gaatacctgg caaaacacgg cacgccgcaa tctacagaag agcttgccgg
     1201 tcaccaatgt ttaggcttca ccgaacccgg ttctctaaat acatgggcgg ttttagatgc
     1261 gcagggaaat ccctataaga tttcaccgca ctttaccgcc agcagcggtg aaatcttacg
     1321 ctcgttgtgc ctttcaggtt gcggtattgc ttgcttatca gattttttgg ttgacaacga
     1381 catcgctgaa ggaaagttaa ttcccctgct cgccgaacaa acctccgata aaacacaccc
     1441 ctttaatgct gtttattaca gcgataaagc cgtcaacctc cgcttgcgcg tatttttgga
     1501 ttttttagtg gaggaactgg gaaacaatct ctgtggataa ttctataaaa acctgccata
     1561 aaattgtttt tactcactta ataactttga gaaaaaataa ataagatgat gtatagccgg
     1621 cggcaatggc agtctatatg gggagcgtga tagggctgtt ccttatgtac ggcttgtgtg
     1681 tggcaacatc ggctttccaa atgacaaaat gccgtctgac accgtaaaaa aaggtttcag
     1741 acggcatttt caacctgatt caggcatcag ttgcggtttt tcaaacgccc gtgcaactct
     1801 tgaacgctgt acacgcccag atggtctcgg cttttcgcac cttcgctcat cgcttcgccg
     1861 ccggcagtgg tggtgtattg cggcacacgt tgctgcaatg cgctttggcg gatgatgtgg
     1921 ctgtcggaca cggattgcgg atcgctggaa acggtattca cgaccagtgc gatttcaccg
     1981 tttttcagtg cgtcgccgat gtgcgggcgg ccttcgggga ctttattgat ggtttgtaca
     2041 atcagtccct gttcggtcag gtattgcgcc gtgccgcgtg tggcgcagat gccgtagcct
     2101 aaaacctgga agtttttagc ggttttgata acgcgttctt tgtcttcttc gcgcacggag
     2161 aggaagattt tgccggtcgg gttgaggcgt tcgcccgcac cgagttgggc tttgtagtag
     2221 gcttcgccga agcttgcgcc cacgcccatc acttcgccgg tggagcgcat ttccggtccc
     2281 aaaatcgtgt ctacgcccgg gaatttgatg aatgggaaca cggcttcttt aacggcatag
     2341 aaatcgggga caacttcttt ttccacgcct tgttctttca gggaaatacc tgccatgcag
     2401 cgtgcgccga ctttggcgag cggcacgccg gtggctttgg agacgaaggg cacggtacgg
     2461 ctggcgcgcg ggttcacttc caacacgaac accacgccgt cctgcacggc aaactgcacg
     2521 ttcatcagac cgaccacgcc cagcgcgtac gccatcgctt tggtttggcg gcggatttcg
     2581 tcttggattt cttcgcttaa ggagtagggc ggcagcgagc agccggagtc gccggagtgg
     2641 atgcccgcct gttcgacgtg ctgcataatg ccgccgataa ccacgtcttt gccgtctgaa
     2701 acgcagtcca catcgacttc aatcgcgttg ttgaggaaga aatcgagcaa cacggggctg
     2761 tcttcggaaa cctgcacggc ttcgcgcatg tatttttgca aggcttcggc agagtggaca
     2821 atctgcatgg cgcggccgcc caaaacataa gacgggcgca cgaccagcgg atagccgatt
     2881 tcttcggctt tgacgagtgc ttcttcttcg ttgtgggcga tgcggttggg cggttggcgc
     2941 agacctaagt cgttcaacac tttttggaag cgttcgcggt cttcggcggc gtcgatgctg
     3001 tcggcggatg tgccgatgat gtttacgccg ttttcaacca gcgcgttggc gagtttgagc
     3061 ggggtttgac cgccgtaatg cacaatcacg ccccacgggt tttcggtgcg gacgatttcc
     3121 aacacgtctt ccaatgtcag cggctcgaaa tagaggcggt cgctggtgtc gaagtcggtg
     3181 gacacggttt ctgggttgca gttcaccata atcgtttcaa agcccgattc gcgcagggcg
     3241 agtgcggcgt gaacgcagca gtagtcaaac tcgatgccct gaccgatgcg gttcgggccg
     3301 ccgccgagaa tcatcacttt tttacggtcg gaaggacggg attcgcattc ttcttcgtaa
     3361 gtggagtaaa gataggcggt ttcggtggcg aactcggcgg cgcaggtgtc aacgcgtttg
     3421 taaaccggat gcagcttcag cgcgtagcgg tgttcgcgca cttctttttc gcttacgttc
     3481 aacaattgtg ccaaacgttt gtcggagaag cctttgcgtt tcagacggcg tagggcggcg
     3541 aaatccaaat cactcaaaat gccgtctgaa accgcttttt cttccttcat caagtcttcg
     3601 atttgcgcca ggaaccaagg gtcgatggcg cagatttcgt ggatttcttc cagcgtgaag
     3661 ccggcgcgga acgcgtctgc cacaaacagc atacgttcgg ggccggggtt tgccagttcg
     3721 cggcggattt ccgctttgtc ggagcttctc ggattgaagc cgcacaagcc tgtttccaag
     3781 ccgcgcaggg ctttttggaa gctttcctga atcgtgcggc ccatcgccat cacttcgccc
     3841 acggatttca tctgcgtggt caggcggtcg tctgcggcag ggaatttttc aaacgcaaaa
     3901 cgcgggattt tggttaccac atagtcgatg gacggctcga atgacgcggg ggttttgccg
     3961 ccggtgatgt cgttgcgcaa ctcgtccagc ttaaagccga ccgccagttt cgccgccacc
     4021 ttcgcaatcg ggaagcccgt cgctttggaa gccagcgcgg acgaacggct cacgcgcggg
     4081 ttcatctcaa tcacaatcat ctcgccgttt tcagggttca ccgcaaactg cacgttcgag
     4141 ccgccggtat ccacgccgat ttcgcgcaat actgccaacg aagcattacg catgatttgg
     4201 tattctttgt ccgtcagcgt ttgcgcaggc gcaaccgtaa tcgagtcgcc cgtatgcacg
     4261 cccatcgggt cgaagttttc aatcgagcag ataatgatgc agttgtcgtt cttgtcgcgc
     4321 accacttcca tctcgtactc tttccagccg agaacagact gctcaatcag cagctcgtgc
     4381 gtaggcgacg catcgaaacc gcgttcgcaa atcgccaaaa actcatcttt attgtaggca
     4441 atgccgccgc ccgaaccgcc catcgtgaaa gacggacgaa tcagggtagg gaagccgacc
     4501 tgttcttgcg ctgccaaagc ttcgttcatc gtgtggcaga caaaagattt cgggcaggag
     4561 aggccgattt tttccatcgc ttctttaaaa cgaccgcggt cttccgcctt gtcaatcgcg
     4621 tcttccgtcg cgccgatcag ctcgacattg tatttcgcca gcacgccgtt gcgcgccaaa
     4681 tccagcgcac agttcagcgc ggtctgaccg cccatcgtgg gcagaatcgc atcaggacgc
     4741 tccttggcga taatcttctc caccgtctgc cacataattg gctcgatgta ggtaacatcc
     4801 gccatttcag ggtcggtcat gatggtggcg gggttggaat tcaccagaat gactttatag
     4861 ccttcttcac gcaaagcctt acaggcctgt gcgcccgaat agtcaaattc gcaggcctga
     4921 ccgataacga tagggccggc gccgatgata aggatggatt ttaggtcggt acgtttgggc
     4981 atgggtggtt actcttgaat taaagaaggt tgttttgtta catagctttt aagaaaagta
     5041 tttccctttt ctaaaattga agctaaaaca tcatcttcta tgttagataa aatattctct
     5101 aaatctgcgc ttgatttaat tttaaagaat tcttttgttt gagaatggta tacaagagaa
     5161 ggtttattta gctgtaaaag ttctgctata ttatttaatg aaccaatact cttaccatcc
     5221 cataaaataa agccataatc agcaatttct gccattttct tatcttttgc tgtataaaat
     5281 acccttccag ttcctttgct gtctacttgg acaaactgcc aattccccac attatttcgg
     5341 taaattttgc cagaaaagta aatatgcaca ttagcataat cttgttcttg tagaaatttt
     5401 tggatagctt tatcagcccc attagcatct ccaataacaa tatcaaaatt attgctaaga
     5461 atattgttaa ttctttctcg tatttgtgga tttagtcgtg aaatgctgcg tgaaccagaa
     5521 aagaaaatag tgcgcatttt tatcctctag tctttgtcat tgctaaaaca attacagatc
     5581 tagcattttt tgaaaacaat ttttcagttg atatttccaa tgttgctccg ctatcaaata
     5641 aatcatcaat tactaaaata ttcttattgg atagatctat attgtcaatt gtgatggaat
     5701 tattcagtat ttctagtttt tctgattttt cttcaatatt tttgagtggt gtgtgtgagc
     5761 tagattttct taaaatagga gaatatggaa tattcaagcg atcacttagt tctttggcaa
     5821 ttaattgaac tggttgatta atacgctctg ttgtaaaagg ggcgggaaca attaaattta
     5881 tagattctaa accactaaat ttttgtaaaa tatagtctac cagcaaagaa acattttggg
     5941 tttgattacg atattttaat tgataaaccc attctccaat cacacttctc ttgctatcaa
     6001 agtgaggatg ccctaattca tcataaccta acaaaatact tttttgcata tgatggtcta
     6061 aagcaaatcc ttttgtccag ttaccattta gttctatcgc catttcattg actccttgtc
     6121 aaaattaagt ataacgagca atcctagcaa gccgtagccc gcatgtaggg tatgtgcggt
     6181 acgcacgcac gcgttcttca ttttccctgt aaccccaacc caccgcatgc gcgccttgcg
     6241 gcacacactc tgccgacgga ttcaaaggtc gtctgaaaaa acgctcgcgg cgtttgttac
     6301 atctgccttg cgataaaggc tgcctgaaaa attcccactg ttgatatttc gggcattctt
     6361 tctcaaaccg cctgcaaccg caaaatctgc tgaaacctac tccctccccc gtgggggagg
     6421 gctggggaga gggcattctc cgaatttcgg caacctttcc taacactcta accgccaaga
     6481 cacaagcctt gcggcttgtt gccctctctc taacgctttc ccacagggag agaggacggg
     6541 gcggctgttg gaattaaggg ttctgcagga aaaacaggtt gtgcgggctg ttttttgttt
     6601 ttcagacggc ctttataaat gctcaattca gtcggcttta tcctattatt ttcaataatc
     6661 caatcaaaat cagccatctg ctatcgtgca gcttgctttt ccaattcttg caacacgagc
     6721 aggatttctg tcagcacatc gtttgtctgc tgtaaaatct cgtgattcca aaaccgcagt
     6781 acggtaaagc ccaagctgtt gagatatgcc gtccgcgcgt ggtcgtatgc ggcttgttct
     6841 gtgtgctgcc ctccgtctgc ttcgacaatc aacttgggcg ttacgcacat aaaatcaaca
     6901 atatagtttc ccatcggctg ctggcggcgg aatttatagc cgttcagacg gcctgcccgc
     6961 aggtgctgcc acaattttgc ttccgcctcg ctcatttctt ggcgcatggc tttggcgcgt
     7021 tgatgcagga cggggttgtc ggcggttaaa agtttctcgt gcgtgctcat tctgttgtcg
     7081 ggttactgtt tgtgtaagcc ttacagtttg ttgccctctc tctgactctc tcccatggga
     7141 gagaggacgt ggtggcggtt gggattaagg gttctgcaag gaaaacaggt tgtgcaggct
     7201 gctttttatt tttcagatga cctgaaacaa cagctgcaaa tctaagttgc ctttcaaatc
     7261 gcctgcaacc gcaaaatctg ctgcaacctt ctccctcccc cgtgggggag ggctggggag
     7321 agggcattct tcgaatttcg gcaaccttcc ccaacaccct aaccgcccaa acacaagcct
     7381 tgctgtttgt tgccctctct ctaacgcttt cccacaggga gacaggacgg ggcggctgta
     7441 gtgattaagg gttttgcaag gaaaacaagg aaaacaagtt gtacaggctg ttttttattt
     7501 ttcaagcgac ctgaaatcaa cgcttgtaaa tccaagtcgc ttttcaaact gcctgcaacc
     7561 gcaaaatctg ctgcaaccta ccccctcccc cgtgggggag ggctggggag agggcatttt
     7621 ccaagttgcg gcaatctttc ccaaatccct tagtcttcta aaatacaagc cttacggttg
     7681 ttgccaaatg aaacttcttc catacccaaa acttgcgtgt aaaacgcaat ggttcggtca
     7741 atgtcggcaa cggtcagtac cagatggtcg agtgcgctaa tcttcatttc ccgctttctt
     7801 tttcaggcgg cggcagtgcc gtctgaaagc cattatgccc gttttgccgc tttcatattg
     7861 ccaatgaatt tgtcaaacaa atagccgaca tcttgcggac cggggctggc ttcggggtgg
     7921 ccttggaagc agaacacagg tttgtcggtc agctcgatgc cttgcaaagt gttgtcaaac
     7981 aaggatttgt gggtaatgcg tgcgttagcg ggcagggtgt cggcatcaac ggcaaaaccg
     8041 tggttttggc tggtgatgac gactttgccg ctgtccaaat cttgcacagg gtggttcgca
     8101 ccgtggtggc tgaagcgcat tttcagggtt ttcgcgccga tagcgaggct gatgagctgg
     8161 tgtcccaagc agatgccgaa aatcggtttg ccgctttcca tcagtttttg cacggcttcg
     8221 atggcgtagg tgcaaggctc ggggtcgccg gggccgttgg acaggaacac gccgtcggga
     8281 ttgagtgcca acacgtcttg tgcgctcgtt tgggcgggga cgacggtcag gcggcagccg
     8341 cgcgaggcga gcatacgcag gatgttggtt ttcacgccga aatcgtaggc gacgacgtgg
     8401 taaggctgtt cgtcaggggt aacgaaaccc ttgcccaatt cccattcgcc ttccgtccat
     8461 tcgtaagttt ccgtgcagga aacttctttt gccaagtctt tgccgaccat actgccgaac
     8521 gcggcgatga gttcttgcgc tttttcaacc gtagcatccg cgccggtcaa gatcgcgccg
     8581 ccttgcgcgc ctttttcacg caacagcgtg gtcaggcggc gggtgtcgat gtcggcgatg
     8641 gcgacggttt tgttgcgtac caaatagtcg tgcaggcttt cggaggcacg gaagttgctg
     8701 tgcaagagcg gcaggtcgcg gataatcagg ccggcggcgt aaacgctgcg gctttcttca
     8761 tcttcggcgt tggtgccggt gttgccgatg tgggggtagg tgagggtaac gatttgtttg
     8821 cagtaggacg ggtcggtcag gatttcctga tagccggtca tcgaagtatt gaacacgact
     8881 tcgccggaag tcgaaccttc gtaaccgatt gatgtgccgt ggaatacgct gccgtcagcg
     8941 aggacgagga gggcgggggt gctcatgatg ggaatcctgt ttttaataaa attcttgaca
     9001 atgccgtctg aagggacttc ggcagggggc atccggtcgg aaaaatccgg tcaaaaaaaa
     9061 acacgccgcg caagatagac gcgtaacgtg cttttcgggc aagtgcctat accctgaaag
     9121 cagcatattt taagacgaaa catacccgat ttcaagcgca aacgcggcag gcgggctgat
     9181 atgttcgcgc gaaaacggaa atccgtggcg taaaaccgcc cgctctgcgc ccactatgcc
     9241 cgttacgcat ttccccacgt ccgtccggcg aaactatggg aatacccgaa tcgtcattcc
     9301 cacgaaagtg ggaatcca
//
LOCUS       DAWEWP010000073         9301 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWEWP010000073 DAWEWP010000000
VERSION     DAWEWP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.073"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            40..645
                     /locus_tag="WGN53_001239"
     CDS             40..645
                     /locus_tag="WGN53_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ0140233.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QLSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            708..1070
                     /gene="ssrA"
                     /locus_tag="WGN53_001240"
     tmRNA           708..1070
                     /gene="ssrA"
                     /locus_tag="WGN53_001240"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1387..2136)
                     /locus_tag="WGN53_001241"
     CDS             complement(1387..2136)
                     /locus_tag="WGN53_001241"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0140234.1"
                     /translation="MPSVELLLEIVGLPRGTFYYQLAVQSAEDKYADLKRHIHDIYRR
                     HKGRYGYRRIAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEI
                     LKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWF
                     KFNPLYNNDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(2141..2551)
                     /locus_tag="WGN53_001242"
     CDS             complement(2141..2551)
                     /locus_tag="WGN53_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ0140235.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            complement(2548..2778)
                     /locus_tag="WGN53_001243"
     CDS             complement(2548..2778)
                     /locus_tag="WGN53_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140236.1"
                     /translation="MVSNPATGKISHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGA
                     MVGGYALIKRGIAAVPQLAEVKKSANQEGGRQ"
     gene            complement(2768..2977)
                     /locus_tag="WGN53_001244"
     CDS             complement(2768..2977)
                     /locus_tag="WGN53_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140237.1"
                     /translation="MSKKSILALMAAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKSVTNNRLFPMAGRLGL"
     gene            complement(2988..3491)
                     /locus_tag="WGN53_001245"
     CDS             complement(2988..3491)
                     /locus_tag="WGN53_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ0140238.1"
                     /translation="MSDEFNRFIERVLSHEGGYVNHPQDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            complement(3581..4063)
                     /locus_tag="WGN53_001246"
     CDS             complement(3581..4063)
                     /locus_tag="WGN53_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140239.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            complement(4060..4971)
                     /locus_tag="WGN53_001247"
     CDS             complement(4060..4971)
                     /locus_tag="WGN53_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140240.1"
                     /translation="MFDLEVALRRVLSAAPTVQDTQIEQYNQLTQIMHRTLDPKDVKG
                     NSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPPSGYSFLRKEAFLYIVGHHV
                     IFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFKAVGNCDKLSLIQQHGVKSI
                     LLDVSAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSDEELEAQSEIHINLEVLLNG
                     NSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVIQPSDVKLSKSVRIGRYDEA
                     NSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            complement(5261..5506)
                     /locus_tag="WGN53_001248"
     CDS             complement(5261..5506)
                     /locus_tag="WGN53_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140241.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            complement(5535..5912)
                     /locus_tag="WGN53_001249"
     CDS             complement(5535..5912)
                     /locus_tag="WGN53_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0140242.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            6026..6205
                     /locus_tag="WGN53_001250"
     CDS             6026..6205
                     /locus_tag="WGN53_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0140243.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            6217..6507
                     /locus_tag="WGN53_001251"
     CDS             6217..6507
                     /locus_tag="WGN53_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0140244.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            6552..7427
                     /locus_tag="WGN53_001252"
     CDS             6552..7427
                     /locus_tag="WGN53_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ0140245.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(7449..7760)
                     /locus_tag="WGN53_001253"
     CDS             complement(7449..7760)
                     /locus_tag="WGN53_001253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140246.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLMLTASSLRYSVARLMPNSCACCLRYAERAPRPIAS
                     A"
     gene            7739..8290
                     /locus_tag="WGN53_001254"
                     /pseudo
     CDS             7739..8290
                     /locus_tag="WGN53_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8384..8647
                     /locus_tag="WGN53_001255"
     CDS             8384..8647
                     /locus_tag="WGN53_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140247.1"
                     /translation="MADILPSDKQRQPPKTKGNPMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMIAATGLVTAIAAVLALIFKFA"
     gene            8684..8965
                     /locus_tag="WGN53_001256"
     CDS             8684..8965
                     /locus_tag="WGN53_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140248.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            8977..9189
                     /locus_tag="WGN53_001257"
     CDS             8977..9189
                     /locus_tag="WGN53_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0140249.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
BASE COUNT         2552 a         2370 c         2137 g         2242 t
ORIGIN      
        1 ccgcccgaaa cagccatcgg acaaataaca ggaaaaaata tgaataccga cttccaaaac
       61 atcctcaaac aactgggcaa acaagcccaa aaagaagcgg cagaaaaaca ggcagccgaa
      121 aaaaacaagc aaaaacaaga acaggatttc gatttttcac aagcagtcgg acaactctcc
      181 ccattaaaaa accggcagca atattatgcg ccgcccgaca aaacccccat caaagtccgt
      241 cccaaagaca accgcgccga cgaagaaaac tacttctaca tcggcagcac atacaacgac
      301 ccgcccgcca gcttcagcaa aaacggacag ggaaaaaacg acatccaacg cctgaaaaac
      361 ggatactatc ccgtcgttac cgatgtcgac ctgcacggct acacacagga agaagcccaa
      421 aaagtcctca acgaattcat cgcattcacc caaaaacgcg gcgtatgcgg cgaaatcatc
      481 cacggcagcg gattaggttc caaaggctac aaacccgttc tgaaaaatat gacccgaaac
      541 tggctgatgc aacaccccga cgtactcgcc tacgccgaac cccgggaagg caacgacggc
      601 tgcgtccgca tcctgctcaa acgcaaatta aggcagcaag actgacaaat ttctccgaca
      661 gacttggcag caccgcacaa tatccgtata atttgcaaac catttttggg ggcgaccttg
      721 gtttcgacgg gggttgcgaa gcagatgcgg gcataccggg gtctcagatt cccgtaaaac
      781 actgaattca aatagtcgca aacgacgaaa cttacgcttt agccgcttaa ggctagccgt
      841 tgcagcagtc ggtcaatggg ctgtgtggcg aaagccaccg caacgtcatc ttacattgac
      901 tggtttccta ccgggttact tggcaggaaa taagacttaa ggtaactggt ttccaaaagg
      961 cctgttggtc ggcatgatgg aaataagatt ttcaaataga cacaactaag tatgtagaac
     1021 gctttgtaga ggactttcgg acgggggttc gattcccccc gcctccacca taccctattt
     1081 tcagcagcat cctgcaacac aaaaaagcct tgaacttcaa cagtttcaag gcttttttgt
     1141 gttgcaggat gctggcgtat gcttgagttt tcgccattta tggggtaact tttggggtaa
     1201 cttttcacga attaccccaa aaaacgcctt aaaaatgcaa aagtcatcac gcattttttt
     1261 aaaaaggtta cccaaaactt aaatgtaatc ctttatttat aagcttgcag ctttccaaac
     1321 aagttacccc caaacggata aaaaaatgcc gaagccgaac tgaccccaaa aagtcggaca
     1381 gtttaatcaa gcggctttca gggactgaat tctgtactga acaaggctca gcccttttaa
     1441 tttcaacttg attctatcgt tgttgtatag tggattaaat ttaaaccagt acggcgttgc
     1501 ctcgccttgc cgtactattt gtactgtctg cggcttcatc gccttgtcct gatttaaatt
     1561 taatccacta taaaaaaaca tctgatattg ccaaccttga tccgaatgca gtatcggctt
     1621 ttcagacgat cccggtttct ccggcgcacc tttcagtatc tcgccggcca aatcgaaagt
     1681 cgggcggatt tgaatacggt aactgacgat ttccccgtta aacaaatcca taatcggaga
     1741 aaggtatatc ttttctccgc ctacattgaa ctcggtaacg tccgttaccc atttctcatt
     1801 cggcttttct gcatggaaac agcgtcgcag gatattcggc gcaattttgc cgacttctcc
     1861 tttgaacgag cggtatttgc gccgccgtat cactgccttc agccccgtct tcgccatcag
     1921 acggctgact ttcttgtgat tgaccggtgt tcctgcgtga cggatggctg ccgcaatcct
     1981 ccggtagccg tatcttccct tatgtcgtcg ataaatatca tggatatgcc gtttcaaatc
     2041 ggcatatttg tcttctgccg attggacggc caattggtaa tagaaggtgc cgcgtggcag
     2101 tccgacaatc tccaacagca gttcaacgga tggcattgcc ttaaccctgc gacgagttgc
     2161 gttctttctg ccgcacttct ttcccatgga ttaaggcatc gggctttttt agggcagcca
     2221 tttccgctgt aaggcaagcc aattccgcaa gcagttcttc cttggttttc agatagccgg
     2281 cttttttcgt ttgcggcgga tactgttttt tcacgggctt tcttccttgt ttttgggaaa
     2341 agtcgtacca ccgctgcctt tcgtcctgcg cttttttaag ctgcaaggca tattgttccg
     2401 ccgcaaccat agaagaatgc cgataggtgg caatcagggc ggtttgttcc gcttcggcct
     2461 tgtcggcggc gtatcggtaa ccgcacgtcc acacaagcaa aagcacaaat gcccaaagca
     2521 gccgtttttt ggctcggaca aattcaatca ttgccgcccc ccttcctgat ttgcggattt
     2581 tttgacttcc gccaactgcg gtacggcggc gatgccgcgt ttgattaatg catacccgcc
     2641 gaccatcgcg ccgtatgccc accaaagcca ttcgggcgcg tccgccgcct gcacgaactt
     2701 ccaagtcatg gcggctgcgg cgacgtttgc ccaaagtttg gtatggctga ttttccctgt
     2761 ggccggatta gagaccaagc cgcccagcca tcggaaaaag gcggttattt gtgacgcttt
     2821 ttttggctgc gcgtttcgcg gcggctaccc cgctcttgcg gtgggcttgc gtccaacatc
     2881 cctgagtcgg catggcgtag cgaatgccta ggtcgctgtg gctgaaatcg ggctgcatgg
     2941 ctgcggccat cagggcgaga atggactttt ttgacatggt ttgctcctca atcggcattg
     3001 tctgcggcgg cgtgaatcag gttttgcgcc acacggcgta cccagccctt gccgaaagag
     3061 gtgaacgtac ccagcttggt ataaaagacc agacgctcgg cgttgaaccg caataaaagg
     3121 tcgttttcgg gaagggaatt gatggctttg aggctgattt cgccgatgat tccatcgtcc
     3181 ggcacgcctg cggcgcgttg gagcatacgg gcggcattgc cgtaaccgtg gttgacgcag
     3241 gcatcaaaaa attggaacgc gaccgcttcc ggcatttggt cggcgcggta acgctcccaa
     3301 aacgctttac ggtaaatgct gattgcctgt tcacgcgtca tggcacgcat ggagccgttg
     3361 tagccgtttg cctgggcggt gcgcttagtg atgccccaat tggtttcgcc gccggggtct
     3421 tgagggtggt tgacgtaacc gccctcgtgg gagaggactc gttcgataaa tcggttgaat
     3481 tcgtctgaca tggaaaaatc cctgtattga attaaaaatc aatacaggga ttttagaaaa
     3541 ggtcgtctga atgggatttt aaagggggtt aaaactcgtt ctagattttg ggtttcttct
     3601 tttccattaa ttttttaaac tcttcttttc ggcttttaat taaattccca ggtaagaata
     3661 cagaataaaa aatggaaata caggctaaaa ataaatagct ccgttcaagc caaaaaatgg
     3721 ctggtccatt tttgtattct tctttatgag ccaacaagat gacaataaaa acacaaacta
     3781 atacggcaag atatgtgtaa aaaagtaagg aatgttttat ataccgttgt tcttgtacgc
     3841 cttcacgaat ttgaatttgt cgccaatttg catcttcatc aaacctcata tcactaaaca
     3901 atgtcataat ggcaattaaa aaaccggcta agattgcaaa tacgttaata attaaatcaa
     3961 cggccttctc gttcccatga acaaaaggtt gccctttcca tgctaaaaat gctgatatac
     4021 aagcaatcaa caaaaaacgt agtaagggca gatatttttt cattgttcag ttaagtttct
     4081 gctttggagt tctaggaaat attcactgat tgctgtaaat gccgagtcag ggagtaacga
     4141 gttggcttca tcgtatcgtc ctattctaac agatttagac agttttacat cagagggctg
     4201 aatcacttca ccttgttgcg tggtaatgga aaatccttga ttaaccgttt catcatcaat
     4261 aatttcctct gcctgctcct gcatcagcga ttgcgcttca atactggctc tcgaattacc
     4321 gtttagcaag acttctaaat tgatatgaat ttcggattgt gcttccaatt cctcatcact
     4381 caattcgctt gtaaatacac taccaacttt ccctaatgct ttagcaatgg ttgaacggct
     4441 atcttgatac agtctgtttc tggataattg atatgccgaa acatctaaaa gaatactttt
     4501 taccccatgt tgttgtatca atgacaactt gtcgcaatta cctactgctt tgaattcaat
     4561 attaaattga acaatatccg gattgttctc tctcaattta ttgctaagta aacttaaata
     4621 agaagcaacc gttgatgctg acaaaaaacc atgtccgcaa aaaattacat ggtgtccaac
     4681 aatatacaaa aaagcctctt tacgcaaaaa ggaatatcca cttggaggag cttgtgttcc
     4741 accatgatca tcttgctgta caggtctatt gctcatagtg cggatatgtt catcttttgt
     4801 accgctgcca atgtgtatca gtaatccacc aattgaattg ccttttacat ctttaggatc
     4861 caaagttcta tgcatgattt gggtaagctg attgtattgt tcaatttgtg tatcttgaac
     4921 agtaggagca gctgacaaaa cccttcttaa tgctacctct aaatcgaaca agctatggtt
     4981 ttgctgcaca cgtaaataat gaatagtttt agtagttttt ttgccatttg tacagtcctt
     5041 tctcttttaa tatctgtgtc gaaaaaataa attcagacgg catcaattca aatctttcaa
     5101 cacctgaacc acctgcccga tgacctgtat gtcggggtag tttttaatgc gttttaaaat
     5161 gcacggccgc ttcaattgct taatatccgt ttcagaacac cggagcagcc cgactgtcgg
     5221 agtgtcctaa atgccgtctg aaaatttcag acggcatttt ttatttttca aatagctctt
     5281 tgccgattgc ttggtaatct acttcttttt cagttggatt taccgtgtct gcataggctt
     5341 ctaccaaaga aaacacacca gcttggctta gataatgcac ttttaccgcc ttagctaatg
     5401 ccgaagccaa aggttcggga atttcgatat tgttctgggt ggctgcctct ttaacgattt
     5461 cttccgctcg ctcaccccat ttgatttgtt gttcaaagtc aaacataaga ctgtctccat
     5521 tttggattta aaaatcacgc tgttttatgc gtggctgaaa cttgagtatt ttcggctgct
     5581 tttttctctg cgccttttgc cacactgaga atgacaaatt tgccgttatc gctggcttgg
     5641 cggtaatacc ctaataactc tgactcttcc ttactaattt cagaattttc agacggccta
     5701 attccctgtt cagcagcaaa taagctgctc acatctattc ccatattcaa aaaagagagc
     5761 aatacatctc cactaggcat aaatacgcca cgttcgtatt tgccccatgt ttcgcgctca
     5821 atacctgctt tttcagcagc ttgtgcttgg gtaagcccta aaaactttct tttttctttc
     5881 aaacgattac caaaaagaga tttagaaatc acaaaataat ccttgcaaaa gagattttaa
     5941 aatctcataa ttcacgcatt ggtaatgcac taattaattt ctagtgcata aatagttcac
     6001 tattttatca caatgggaaa gggctatcta tggctatact gttgaaaaac taaaagaaaa
     6061 ttttgcccaa aacggccaaa cgctggcgca gtgggcaaga gagaacggct ttaagccgcg
     6121 cgatgtgtat ctggtggtcg gcggacagcg caaaggcaat tacggcaagg ggcatgaaat
     6181 tgccaagaag ctgggactga aataaggagg atgcggatgg cagaaagtaa aagggtacaa
     6241 cggctattga gggtctttat cgcgcttgac gagcatccga ttatcggtct gagcaataag
     6301 gatttatcgg tcggactggg gctgacgcca tcgcaagtca gcagggatat tgatgatttg
     6361 gttgcctcgg gattggtcat caaacttgaa aacggcaact acgcctacgg catcaaaacc
     6421 ctgcaaatcg cagagcggtt caggcagcag catgaacggc tgcaaagcaa gattgccgaa
     6481 atcggcaagc gggtcgatgt ggattaacgg attttgaaag tttccgacgt cggaaacttt
     6541 ggagataaaa aatgaataca gaaattgaaa ttatcgcaac cgacaaagta agcaatcagg
     6601 cagcgatgcg cagtgtgctg gtgatggagc agtggggtaa cggcgagact tatagcgaag
     6661 agcgttgggt tgaacgcggt cgtcaagcag tacggcagac gatggaaggc atgttcgaac
     6721 tgggacgtgc gctgattatt ttaaaagagc atacggaaca cggacggttt atggaaattg
     6781 tgaaaagcca atttgggctg ggtatcgcag aaacatcacg tctgatgtca gccactcgcc
     6841 gctttgcgac cccgcaaatg cagaaagccg cgccgaagct gatggatttg ggcaagtcaa
     6901 agctgctgga actcttggtc gaagaagacg ttacgttggt gggacttgcc gaaggagaag
     6961 aagtcaacgg catgaccttt gacgatgtgg accgaatgac ggtgcgcgag ctgcgcgtcg
     7021 ccctgcgcga aagccgtgaa aacctcgccg ccaaagacga agtgatgaaa accaaaacag
     7081 ccaaaattga tgagctgacc gagaagctgg ctaagaagca aaccgttgtc agagagccga
     7141 aggcggaaga cgtgggcagt gagttggcga tgcagttaac aagccttgag gtcggtatcc
     7201 gcagtcaagt gagccgtttg aaagatttgt tcgaccaact taatgcgcac agtgaggcac
     7261 acgagatcag ccatcaggca aagatggtcg gcacgctcaa tcaaattatt ttggactgcg
     7321 agcaactgcg cgaaagctat gccctgccga ctgaagcacc gacagacaat gtgccggaat
     7381 ggttgggcgg tgaaacggga gaaggcgatg aatccggcaa tgattgagcg tcttaaggca
     7441 gtcgagaatc aggcggaagc aatgggacgc ggcgcacgct ctgcatatct taagcagcag
     7501 gcgcaggaat tgggcatcag ccttgccacg ctataccgca agctggaggc ggtcagcatc
     7561 aagccgacgc gcaaacggcg cagcgatgcg ggcaagacgg agcttaaacc ggaagaagcc
     7621 aaattgattt cggcggtttt ggtggaggcg atgaggcgca acggcaagcg gttgatgtcg
     7681 gtgcggcagg cggtggaaat gctgcgcgcc aacggaaaaa tcgaggcggc gcggattgat
     7741 ggggaaaccg gggaagtcat cccgtccgtc cgcactgctt tccgcccgct ggcaagacat
     7801 cgacctcacc gcccggttat ggcacatccc cgccgccgac atgaagacca aacacccctt
     7861 taccgtccca ctgtcggact gggcggtaga aatactccga gagctgcaca cacagaccgg
     7921 cgacaacatc caccttttcc caggcatccg cccacggcgc aaacccgccc cccatcccga
     7981 ccacatcagc atcaaattcg cccacaacgc catccgacga ctgggctatg acggcagcac
     8041 ccccggcaaa tccaaacaca caatgcacgg attccgccac ctcttcacca acatcagcct
     8101 gaccgccgga aaagacaccc tcacaacaga cctcgccctc ggccacatca gccgcgccgc
     8161 cctccaacgc gccggcttct caagcctgca ccactacctg accgccgaca gctaccgcct
     8221 ccaagagcgg cgcgaactcg ccgaatggta cagccgccgc caccgccaag catacgaagc
     8281 cgccgccaaa accccgcaaa acaaaaaacc gcctgaaaaa aattcagacg gcatcctgcc
     8341 caaaacactt gcaataaaac ctaaaaggta ttaatgtaca cacatggcag acatcctgcc
     8401 atccgacaag cagcggcaac cgccaaaaac gaaaggaaat ccaatgaaca taatcgaaca
     8461 agaaatgaaa cactccagtc tgagaaaagt agatgccgaa atcgccaaaa tcattgcaga
     8521 cgcgcacaaa atcaacgccg aatccgtaaa aatagcccaa gagtcccgct ggtatcccat
     8581 gatagcggcc accgggctgg ttaccgccat cgccgccgtg ttggcactga tattcaaatt
     8641 tgcctgacca caagccccgc gaggggcttt atccctcaac aaaatgaaca gagccgcact
     8701 attcaaccgc taccccgaat ggattatcgg gcaagacggc gcaagccgtt tcattaccca
     8761 ttgccgctat ccccgcctga ttgccaaaat ccacaggcag acagacggcg aatgcccggg
     8821 cgggcattac cgccattccg aaaacggcat caccctttac gattttattt ttttcgacgg
     8881 caaaccggca gacgaagccc gatttgccgc cgtccttacc gaaacctgcc gcagggcagt
     8941 caaaaaaata tgcgaaacaa aataaaccgc aacgacatgg aactcggcta cacgccttac
     9001 aaccttcgca cccttcgcaa ccgctgcaaa ctgacacagg ccgagttggc gcaaatcgtc
     9061 ggggtgaagc actacataca ggtaggccgc tgggaagccg aacccgatac cgaaacccgt
     9121 cgggccgata tgccgctgga aaaatggcgg cagttcctcg attggataga aaaaacaaac
     9181 gccgtctgaa accttcagag tgcgaagcct gcccgccggg tacggcattt tttacccacc
     9241 cccaaaccta aaagccccac gccgcttgcc cccgacccgc cgccgcccca aaataaactc
     9301 a
//
LOCUS       DAWEWP010000074         9269 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWEWP010000074 DAWEWP010000000
VERSION     DAWEWP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.074"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(58..1338)
                     /locus_tag="WGN53_001258"
     CDS             complement(58..1338)
                     /locus_tag="WGN53_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ0140250.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYKTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1560..2180)
                     /locus_tag="WGN53_001259"
     CDS             complement(1560..2180)
                     /locus_tag="WGN53_001259"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ0140251.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAVIDSNLGLDEVRNSIEKVLDGHFGC"
     gene            complement(2240..3235)
                     /gene="mltG"
                     /locus_tag="WGN53_001260"
     CDS             complement(2240..3235)
                     /gene="mltG"
                     /locus_tag="WGN53_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ0140252.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3319..3891
                     /gene="ampD"
                     /locus_tag="WGN53_001261"
     CDS             3319..3891
                     /gene="ampD"
                     /locus_tag="WGN53_001261"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ0140253.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVERLFANRLDPNDHPFFGLIHTLRVSSHFLIKRDGKTVQFVSCDNMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFAEAQYRSLEALLDAICRHYPVTAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFLVDRNAV"
     gene            4087..5373
                     /locus_tag="WGN53_001262"
     CDS             4087..5373
                     /locus_tag="WGN53_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ0140254.1"
                     /translation="MIYIVLFLAVVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5521..8046
                     /gene="ligA"
                     /locus_tag="WGN53_001263"
     CDS             5521..8046
                     /gene="ligA"
                     /locus_tag="WGN53_001263"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ0140255.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMLKADFVALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVVKVNSLAQQHELGFI
                     SRAPRWAVAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKSIEGLQTDKSLPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            8103..>9269
                     /locus_tag="WGN53_001264"
     CDS             8103..>9269
                     /locus_tag="WGN53_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ0140256.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQC"
BASE COUNT         2191 a         2788 c         2330 g         1960 t
ORIGIN      
        1 ctgacagcaa aatgccgcct gaaaacagtt cagacggcat tttaaaccgc aaacggctta
       61 cagcttccat tcgctcagct tggcagcgta agcttccaaa tctgcaatcg gacgggttgc
      121 cacgccgctt tccatcgctg ctttggcggc agccgtagca acgcgcggca gcaggcggga
      181 atcgaacgga gtcggaatca ggtattccgc gccgaattca aatttcttac cgtaagcggc
      241 aaccacttct tcggttactt cttccatcgc caaatccgcc aaagcataca cgcaggcgcg
      301 tttcatttct tcgttgatgg tcgtcgcgcc gacatccaac gcgccacgga agatgaacgg
      361 gaagcacaat acattgttca cttggttcgg gaagtcggag cggccggtac cgataaccac
      421 gtccggacgg gtttctttcg ccagcggcgg caggatttcc ggattcgggt tggccatagc
      481 gaacacgatg ggtttttcgt tcatcgtgtt caacatttcg ggggtcagca ggttcgcgcc
      541 ggacagaccc aagaagatgt ctttgccttt aaccgcatcg gccaatacgc gctggccgtt
      601 gtcttcaatg gcgtagaact gtttagactc atccatgcga tctttgtctt cgcgggtttt
      661 gtaaatcacg ccttttgagt cgcaaacggt cacgttttcg cgtttcaagc ccaaatccag
      721 caattggttc aagcaggcaa tcgcggccgc acccgcacca gagcaaacca aggtcgcttc
      781 ttcgatttta cggccggtaa aacgcagggc gttcaatacg gcagcggcgg taatgatggc
      841 cgtgccgtgc tggtcgtcgt ggaatacggg gattttgcag cgtttgcgta attcgcgttc
      901 gatgtagaaa cactcgggtg ctttgatgtc ttcgaggttg atgccgccga aggtcggctc
      961 taaagcggcg atgatgtcca cgagtttttg cgggtctttt tcatcgattt cgatgtcgaa
     1021 cacgtccaca ccggcgaatt ttttgaacaa tacgccttta ccttccatca cgggtttgcc
     1081 cgccagcgcg ccgatgtcgc ccaagcccaa aacggccgta ccgttggaaa tgacggcgac
     1141 caagttgcct ttggcggtgt atttgtaggc attttgcgga tcggcatgga tttccataca
     1201 aggagcggct acgcccggag agtacgccaa cgccaaatct ttgtcggtcg ccaaagattt
     1261 ggtcggggta acggaaattt tgcccggaac gggtaattcg tggaacttga gggcggcttc
     1321 tttcaatgag ttttccatgt gtcaatcctg ttgcgagaaa aagatttgaa tcggactgtc
     1381 cgaaacgctt tcgggtcatc atgttacggg cggttcgtta atattgccga cgcatcctgc
     1441 cttgtaaccg agcgtaattc tatcataccg aaagtttggc gcataccgcc attttcacct
     1501 gcggcactgc cgtttgattt tcggcgcggg cggatgcgct tgtttcaata tttgccgcat
     1561 taacagccga aatgtccgtc caacactttt tctatgctgt ttctgacttc atccaagccg
     1621 aggttactgt cgataacggc gtaacgttcc ggacaggcgg cggcacggtt cagataaacg
     1681 ctgcgcacac gcatgaagaa atctgcctgt tcctgctcga aacggtcttt ctcgcgcgtc
     1741 tgcccgatac gcgccatcga cacttccagc ggcacatcca acagcagggt caaatcgggg
     1801 cgcaaaccgc cctgcaccca atgttccaaa atttcaatgt cttcagacgg catcccccgc
     1861 ccgccgccct gataggcgaa ggttgcatcg gtaaaacggt cggacacgac atggatgccg
     1921 tctgaaagtg caggcaatat cacgtcttca atatgctgca ttctggcggc gaacatcatc
     1981 agtgtttccg cacgcaaacc ggctttggtt tcagggttga gcaggatttc gcgcaaggcc
     2041 tcaccgaccg gcgttccgcc cggctcgcgc gtgaacagca cgggcagccc cctccgttca
     2101 aaccatgctt tgatgacggc aaggttggtg gattttccgg ctccgtctat gccgtccaaa
     2161 gtgatgaatt gcggtttcat gcttgcagtt ccggtaaggg tatgccgtct gaaaacacaa
     2221 acttttcaga cggcatggtt tattttttca aaatatattt gcggacggcg gcattgtgtt
     2281 cggtcaaatc atggctgaac tggctcaagc ccgtaccgtc cattttagaa acaaaataaa
     2341 ggtatttttc gccagacgga tgggcggcgg catcaagcgc ggccttgccg ggcagcgcaa
     2401 tcggggttgg cggcagaccg ccgcgcgtgt aggtgttgta cggcgtgtcg cggcgcaggt
     2461 cggctttacg gattttgccc ttgtatgccg cacccatgcc gtaaatcacg gacgggtcgg
     2521 tttgcaggcg cataccgatt ttcaggcggt tgacgaagac ggaagcgaca tggtcgcggt
     2581 cggcttcatg ccccgtttcc ttttcgacca ggctcgccat aatcagcatt tcataaggat
     2641 ttttataagg cagcccgtcc tgcctgcttt cccatgcctc attcaggcgg cgttgcatcg
     2701 ccttgtaggc ggtttggtaa atccgtaaat cgctgccgcc cgcatcgatt tcgtagctgt
     2761 cggggaaaaa ctgcccttca ggattgccgc tgaaggcatc aggggcaact tccgccatca
     2821 gtttttcatt gctccagcct ttggtgtcgt gttcgatgtc gggcgttgcg tcgatgactt
     2881 tcctcatatg cgaaaaacgc gaaccttcga taatctgcac ggtaacggaa tccggcctgc
     2941 cgccgcgcat tttctgcaag atatcccaag cagacacttc cgaaggcagt ctgtacgtcc
     3001 ccgtatgcag cctgttgtgc acacccaaaa cgtaggccgc cgccgtcaaa acatgcctgc
     3061 tgaacacgat gcggtcttcg gcaagtttcc tgccgaccga cgaaataccc tggtttttgg
     3121 caattttaat tcggtatgcc ctgccgttgt ctttagggac gaaaagcagc gcggcgaaaa
     3181 cggctgccga tacggtcaaa aaaacggcag accatttcaa caattttctc aacatggtag
     3241 gattcccagc attcggaata cgtcttaaaa ttcgggcgac agtataaccc aaattgcccg
     3301 ccggacagga agaacatcat ggacaatcac gccgaagcac actggcaaaa cggctggctt
     3361 caaagcatac gccatacccc gtcacccaat ttcagcccga gggaaacggg ggaaacggtt
     3421 tccctgattg tgttgcacaa catttcgctg ccgccgttcg aatatggtac ggatgcggtt
     3481 gagaggcttt ttgccaatcg cctagacccg aacgaccacc cgtttttcgg attgatacac
     3541 actttgcgcg tatccagcca tttcttaatc aaacgcgacg gcaaaacggt acagttcgtt
     3601 tcctgcgaca atatggcata ccatgccggc gtatcttcat tcggcggcag ggaaaaatgc
     3661 aacgcatttt caatcggcat cgaactggaa ggctgcgatt tcgaaccgtt tgccgaagcg
     3721 caataccgtt cgctcgaagc cttgttggac gcaatctgcc gccactaccc cgtcacggcg
     3781 gtaacggggc accaagacat cgcgcccggc cgcaaaaccg accccggcca ttttttcgac
     3841 tggcggcgga tacgggagaa agggtttctc gtggacagaa atgccgtctg aaaaccacag
     3901 atttcagacg gcatttcgga aaatttccgc ttatttcgac acactgctgt cagaccctgc
     3961 aattttcgtt ataattcaaa cgttataatc ggccgttccg tcagactgcc ggaaacgcaa
     4021 gcccgattta accgcagtac ccgcactttg cgtatcctgc atcccgaaat ttaacaagtg
     4081 attgccatga tttacatcgt actgtttcta gctgtcgtcc tcgccgttgt cgcctacaac
     4141 atgtatcagg aaaaccaata ccgcaaaaaa gtgcgcgacc agttcggaca ctccgacaaa
     4201 gatgccctgc tcaacagcaa aaccagccat gtccgcgacg gcaaaccgtc cggcgggtca
     4261 gtcatgatgc cgaaacccca accggcggtc aaaaaaacgg caaaacccca agaccccgcc
     4321 atgcgcaacc tgcaagaaca ggatgccgtc tacatcgcca agcagaaaca ggcaaaagcc
     4381 tccccgttca aaaccgaaat cgaaaccgcc ttggaagaaa gcggcattat cggcaactcc
     4441 gcccacaccg tttccgaacc ccaaaccgga cattccgcac cgaaacctgc cgacgcgccg
     4501 gcaaaacctg cacccgttcc gcaaacacct gcaaaaccgc tgattacgct caaagaactg
     4561 tcaaaagtcg aattaccctg gtttgacgtg cgcttcgact tcatctccta tatcgcgctg
     4621 accgaagcca aagaactgca cgcactgccg cgcctttcca accgctgccg ctaccagatt
     4681 gtcggctgca ccatggacga ccatttccag attgccgaac ccatcccggg catccgctat
     4741 caggcattta tcgtgggtat tcaggcagtc agccgcaacg gacttgcctc gcaggaagaa
     4801 ctctccgcat tcaaccgcca ggtggacgca ttcgcacaaa gcatgggcgg tcagacgctg
     4861 cacaccgacc ttgccgcctt tatcgaagtg gcttccgcac tggacgcatt ctgcgcgcgc
     4921 gtcgaccaga ccatcgccat ccatttggtt tccccgacca gcatcagcgg cgtagaactg
     4981 cgttccgccg taacgggcgt gggtttcgtt ttggaagacg acggcgcgtt ccactatacc
     5041 gacacgtcgg gctcgaccat gttctccatc tgctcgctca acaacgagcc gtttaccaat
     5101 gcccttttgg acaaccagtc ctacaaaggc ttcagtatgc tgctcgacat cccgcactct
     5161 ccggcaggcg aaaaaacctt cgacgatttg tttatggatt tggcggtacg cctgtccggc
     5221 cagttgaacc tgaatctggt caacgacaaa atggaagaag tttcgaccca atggctcaaa
     5281 gacgtgcgca cttatgtatt ggctcgtcag tccgagatgc tcaaagtcgg tatcgaaccg
     5341 ggcggcaaaa ccgcattgcg cctgttctcc taaaatcgaa tgcaatcaaa gcggatatgt
     5401 cgttacggca tatccgcttt ttttcgggta aaattcagct gttttcaacc tatcattttt
     5461 cagacgacct ttgcccgttt catcccgtat cagacaaagg tcgtctgaaa accttctctc
     5521 atgaatccaa actcaaaaca taacactaac tttaccaatc ttcttaaacc gtctgatagt
     5581 gatataaaac aatttgccgc acaacacatc cgccacctca ccgacctgct caaccgctat
     5641 gcctacgaat actacaccct cgacgcgccc agcgtacccg atgccgaata cgacaaatta
     5701 ttccgcgagc tcgaagcgtt ggaattaaac catcccgagc tcaaactgcc cgacagtccg
     5761 acccagcgcg tcggcggcga gcctttggcg ggatttgccg aagtgcgcca cgaagtgccg
     5821 atgctgtcgc tgaccaacgc tttttccccg caagatgaaa acggcgtgtt cgaccatgcc
     5881 gaaatgtacg ctttcgacca acgcgtgcgc gacggtttgg acggcggcaa acccgaatac
     5941 gttatcgaac ccaaattcga cggcctcgcc atcagcctgc tctaccgcga cggcgtattg
     6001 gtgcaggcgg caacgcgcgg cgacggcacg acgggcgaag acgttaccca aaacatcaaa
     6061 accgtatcca acatcccctt gcggctgcac ggcgaaaata cgcccgaact catcgaggta
     6121 cgcggcgaag tgctgatgct caaagccgat tttgtcgccc tcaataaaag acaagccgaa
     6181 aacgggcaaa aaccctttgc caatccgcgc aacgccgccg ccggcagcct acgccaactc
     6241 gattcgcgca tcaccgcgca acgcaaactg cactttttcc cctactccgt cgcccgccag
     6301 caagatggtt tcgtcgcgga agaacacatc caggaactcg cctatttcca agcactcggc
     6361 ttcagtctgc ccaatggcaa tttcggctgt ttcaaaaata tcgacgaagt attggcgttt
     6421 tacgaacaca tgcagcaaaa acgccccgaa ctgccctacg aaatcgacgg catggtggtt
     6481 aaagtcaaca gcttggcgca acagcacgaa ctcggcttca tctcccgcgc gccgcgttgg
     6541 gcggttgccc acaaattccc tgccgaagaa gccctgacca ttgtcgaagc gattgacgtg
     6601 caaatcggca ggacgggcgc ggtaacgccg gttgcccgat tacagcccgt attcgtcggt
     6661 ggcgtaactg ttaccaacgc caccctgcac aatcaggacg aagtttcccg aaaagacgtg
     6721 cgcgtcggcg ataccgtcgt cgtgcgccgc gccggagacg tgattcccga agtcgtgcgt
     6781 gtgatttttg aacgccgccc gatgcaggaa accgccgttg ctgtttcaga cggcatcggg
     6841 catcggcagg acgatttgtt tgccgaagca ccggctgcaa accaaaccca atccgttccg
     6901 ctctacaagc cctaccgcct gccgacccat tgccccatct gccgcagcga aatcgaacgc
     6961 gaagaaggcg aagccgtcgc ccgatgcagc ggcggtatgc tttgtcaggc acaacgcgcg
     7021 caaggcttaa tccacttcgc ctcgcgcaaa gccatggaca tcgacggctt gggtgaaaaa
     7081 caaatcgaac agcttgtcgc ccaagacctc gtccgccact tcgccgacct ctaccgcctc
     7141 gatattccga ccctgcaaaa aatgaaggaa acggcggaca aaaccgttgc cgaatcggat
     7201 caaatgccgt ctgaaggcag ttcggtcggt gcgtcgggca aacataaaaa acaaccgacc
     7261 aagtgggcgg aaaacatcct cgcaggcata gaatccggca aaacgcccga actcgcgcgc
     7321 ttcctgttcg cgctcggcat ccgccacgtc ggcgaacgca ccgccaaaac gctggcacag
     7381 gcattcggca cattggaacg cgtccgccgc gcccccgaac ccgtcctcgc ctgcctgccc
     7441 gacatcggta cggtagtggc gcgttccatt gcccacttct tcgcccaagc cgaacagcag
     7501 gcgatgatag acgagctgct cgccgcaggc gttgccccgc aagcccaagc cgtcagcctg
     7561 cctgccgcac aatatgccga accgcaacgc tggattgccc gcctgcccgg tttcaaaatt
     7621 agcgaaaaca aagcccaagc cttatgggaa ctcgccggca aaagtataga gggtctgcaa
     7681 accgacaaat ccctccccgc cgactggcaa gcatggcgca gcgaaccaca aaacgccgcc
     7741 ctactcgaaa acctgaaaac cttcttcgcg caaatgccgt ctgaagacga ggcggcgcaa
     7801 ggttcagacg gcatcaataa agccgtagcg ggcaaaacct tcgtgttaac tggcacactg
     7861 cccaccctca aacgcgacca agcccaatcc ctaatcgaag ccgcaggcgg caaagtttcc
     7921 ggcagcgtgt ccaaaaaaac cgattacgtc gtcgccggag aagccgcggg cagcaagctg
     7981 gaaaaagcca atgccttggg cgtttccgtc ctcagcgaag ccgaactgct taccctgctc
     8041 ggctgaaccc atgccgtctg aaccgccttc agacggcatt gcccactttt ccgaaccaca
     8101 ccatgcacac catttccttt ccaaaccgca cccgactcac cgccctgccg cccctgtcgc
     8161 tttacatcca catcccgtgg tgcatcaaaa aatgcccgta ttgcgacttc aattcccaca
     8221 gcctgaaaaa cggattgccc gaagccgcct atatcgacgc gctgctgacc gatttgcagc
     8281 ttgaattgcc caatatttgg ggcaggccgg tggaaacgat ctttttcggc ggcggtacgc
     8341 ccagcctgtt tcaggcggaa tcgattgacc gtttgttaag cggcgtgcgt tcgctgttgc
     8401 gcttgcagcc ggaagcggaa attaccttgg aagccaatcc ggggacattt gaaatcgaga
     8461 agtttcaagg atttaaagac gcgggtatta cgcggctttc tatcggcgta cagagtttca
     8521 acgacgatat gctctcaagg ctggggcgcg tccacaacgg taaggaagcc ttaaccgcta
     8581 tcgataccgc cttgaaatta tttgataaag tcaatatcga cttgatgtat gccctgccga
     8641 accaaaccgt tcagacggca ttggacgacg tacaaaccgc tatcgctacg ggcgcgaccc
     8701 acatcagcgc gtatcatctg acgatggaac cgaacacgcc gttcggtcat acgccgccga
     8761 aaggtttgcc gcaagacgaa gccgccctcg acatcgaaga cgcggtacac ggcgcgctgg
     8821 aaggcgcggg ttttgtccac tacgaaacat cggcttttgc gaaaccagcc atgcagtgcc
     8881 gccacaattt gaactactgg cagttcggcg attatttagg cataggcgcg ggcgcgcacg
     8941 gcaaaatttc ctatcccgac cgcatcgagc gcaccgtccg ccgccgccac cccaacgact
     9001 acctcgcctt aatgcaaagc caaccgagtg aagccgtcga acgcaaaacc gtcgctgccg
     9061 aagatttgcc gttcgaattc atgatgaacg ccctgcgcct gaccgacggc gtacccgccg
     9121 cgatgttgca ggagcgcacg ggcgtaccga gtgccaaaat catggcgcaa atcgaaacgg
     9181 caaggcaaaa aggcctgctg gaaaccgacc ctgccgtatt ccgcccgacc gaaaaaggac
     9241 gcttgttttt aaacgatttg ctgcagtgt
//
LOCUS       DAWEWP010000075         9188 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWEWP010000075 DAWEWP010000000
VERSION     DAWEWP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.075"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(112..1554)
                     /gene="aldA"
                     /locus_tag="WGN53_001265"
     CDS             complement(112..1554)
                     /gene="aldA"
                     /locus_tag="WGN53_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ0140257.1"
                     /translation="MKQLAMYINGRFENDFNSEWRDVLNPSTEEAIAREPKGGKADVD
                     RAVAAAREAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGTEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDADNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1662..2579)
                     /locus_tag="WGN53_001266"
                     /pseudo
     CDS             complement(1662..2579)
                     /locus_tag="WGN53_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            2752..3552
                     /locus_tag="WGN53_001267"
     CDS             2752..3552
                     /locus_tag="WGN53_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0140258.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            3601..4377
                     /gene="mlaE"
                     /locus_tag="WGN53_001268"
     CDS             3601..4377
                     /gene="mlaE"
                     /locus_tag="WGN53_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ0140259.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSASFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            4428..4922
                     /gene="mlaD"
                     /locus_tag="WGN53_001269"
     CDS             4428..4922
                     /gene="mlaD"
                     /locus_tag="WGN53_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ0140260.1"
                     /translation="MKKNILEFWVGLFVLLGVAAVAFLAFRVAGGAAFGNSGKTYTVY
                     ADFSDIGGLKANAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            4959..5549
                     /locus_tag="WGN53_001270"
     CDS             4959..5549
                     /locus_tag="WGN53_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ0140261.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALTKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            5606..5884
                     /locus_tag="WGN53_001271"
     CDS             5606..5884
                     /locus_tag="WGN53_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ0140262.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            5881..6708
                     /locus_tag="WGN53_001272"
     CDS             5881..6708
                     /locus_tag="WGN53_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ0140263.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            6833..7195
                     /locus_tag="WGN53_001273"
     CDS             6833..7195
                     /locus_tag="WGN53_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ0140264.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7192..7578
                     /locus_tag="WGN53_001274"
     CDS             7192..7578
                     /locus_tag="WGN53_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0140265.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            7575..8063
                     /locus_tag="WGN53_001275"
     CDS             7575..8063
                     /locus_tag="WGN53_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0140266.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8144..8311)
                     /locus_tag="WGN53_001276"
     CDS             complement(8144..8311)
                     /locus_tag="WGN53_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0140267.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(8278..8658)
                     /locus_tag="WGN53_001277"
     CDS             complement(8278..8658)
                     /locus_tag="WGN53_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ0140268.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGTNCEICRGLVVGKNVMMEPECLFYSNNHKFDHS
                     KNALRATRKSVRLRWRTMSGRGTG"
     gene            8668..8794
                     /locus_tag="WGN53_001278"
                     /pseudo
     CDS             8668..8794
                     /locus_tag="WGN53_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8896..9111
                     /gene="rpmE"
                     /locus_tag="WGN53_001279"
     CDS             8896..9111
                     /gene="rpmE"
                     /locus_tag="WGN53_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ0140269.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         2089 a         2627 c         2379 g         2093 t
ORIGIN      
        1 gacaaaaaca tcgatgtcgg tcatgactga ttgccggtga aactgtaaaa atgccgtctg
       61 aacctgaaaa cgggttcaga cggcattttc tatcggggtt ttaaagcggc attaaatgtc
      121 ggtttccaga taaacgactt gggtttgcag atattcttcc aaaccgtgtt tgccgtccgc
      181 gccgccgatg ccggattttt tccagcccgc gtggaaaccc tgcatggctt cgaagttttc
      241 gcggttgatg taggtttcgc cgaattgcag gcggcgggta acgtagaagg cttcgtttaa
      301 attagtcgta taaacagaac tggtcagacc aaactcgcaa tcgttagcca gtgcgatgac
      361 ttggtcgagc gtgtcgaaag cggaaacggg cagcacgggg ccgaaggttt cttctttcat
      421 aatgtccata ctgttgtcgg cgtcggtcag cagggtcggc tcgaagaaat agccgcgccc
      481 ttcggcgcgt ttgccgccgc agaccagttt cgcaccttgt ttgactgccc gttccacttt
      541 ttcggcaacg gctttgacgg cgcgctcttc aatcagcgga cccatttcca gcgcgcctgc
      601 ttcggcttcg gcagggttgc cgtagcgcac gcctttcatg gcggcggtca ttttttcaat
      661 gaacgcgtct ttcaggctgc tgtggacata gacgcgctcg gcgcagttgc agatttgacc
      721 ggtgttgccg acgcgcgaag ccaagatgga tttcaccgcc aaatccaaat ccgcgtcttt
      781 caacacgatg gcaggggctt tgccgccgag ttccagcgaa actttggtga tgttggcgga
      841 agcggcttcc atcacttggc ggcctgcttc gacggagccg gtcaggctga ccatatcgac
      901 ttgcggatgg gcggacaagg cattgccgat ttccgtgccg gggccgttca ccacgttgaa
      961 cacgcctgcg ggcaatccga ccgcatcgac gatttcggcg aagatatggc agttgatcgg
     1021 ggttacgctg ctgggtttga cgacgatggt gttgcccgtt accaaagcgg gacccatttt
     1081 gcgggcaatc aggaagaaag ggaagttcca cggcaaaatg ccggcaatta cgcccagcgg
     1141 acgtttgaac aataaaatgt tttcgcgcgg gcggtcgctt tggatgattt cgccttcgta
     1201 acggcgcgcc cattcggctt ggtaatcgag atagtcggcg gtgaacatca cttccacgcg
     1261 tgccaagtct ttggttttgc caccttcggc aacgatggtg tcggtcagct cgtcggcacg
     1321 ttcgcgtatg ccttgggcga ttttgcgcaa atacgcgccg cgttcgaccg caggcagacg
     1381 ctcccaagcc ggttgcgcct cacgcgccgc cgctacggcg cggtcaacgt ctgctttgcc
     1441 gcctttgggt tcgcgggcga tggcctcttc ggtagacggg ttcaatacgt cgcgccattc
     1501 gctgttgaag tcgttttcaa agcgaccgtt gatgtacatt gccaattgtt tcatttcggg
     1561 ttctccagtt ttgtagtcag atgtagtttt agtttattcc caaataaatt gccgtacaag
     1621 tttctttaca cgcggatttc gggtttgaaa tcaaatgcgg cttaccggtt ttcttttttg
     1681 gtttccgccg gcgtgctgcc gaaatattgc ctgaatgcct gagtaaagct ggaaacgtgg
     1741 cggtagccgc ataaatatgc ggtttcgcct atgcttttcc ccccgttttg caacagatag
     1801 agcgcgtgct gcatttgttt gtggtgcagc cactcgcttg cggtaatgcc gaaatggtcg
     1861 cgcatacggc gttgcagcgt cctttcgctg atgttcagcg catctgtcag tcggttgact
     1921 tggtgtgcgc cgccgtcgaa cgcggcattt agggtgcggc tgaagtcttc agacggcata
     1981 gcgtctgctt ccgccgtttg ccccaccgcc ggcccgatgc cgtctgaaac cgtgtcccac
     2041 aagtccgaca gcagccgcaa cacgtctgcc tcgcggcgta atgttttgcc caaatgcccc
     2101 tttgggacgg cttgcaggca ggatgccgcc aagccgcgca ggttcggggg caaatcctat
     2161 atcctgaccg gttcgcggta aagcaggggt gcgaaacgcg cgtattccgg acgcagcagc
     2221 cattgttcca tacctttaat tgtcattttg accgttttgc cgcctcggta aagatagcgg
     2281 ctgaacagga cttcttcccc gacagcaatt aggacaatct tgccgccgtc cgcatcgatt
     2341 tggaagcggc agcggttgat gccgaagtcc aaactgcctt ccagcaagag cacgaacgac
     2401 acataaggtt cggcgaggcg gctgctgcaa aaatcacacc gtgccgttac tgtgccgccg
     2461 tggatggaaa tgccgttgga caaagtatcg aaacggtagc tgccctccac atagctgccg
     2521 ccgccgcttt tgactagatt gacgaaccgg ctgctgttca aatgttctgc tttttccata
     2581 gttttctgat aaaggttatc atttgaaaga taacattttt cgccacagca aacaatccgc
     2641 cgccgacaac ggcattttca caacatttac cgtccgtatg ccgtctgaaa attgcccgtc
     2701 ttcccgataa gccttaaaat acacgccgtc aaacctaccg tccgtccgta catgagccca
     2761 tcccccttta tcgaaatgaa agacgtcgcc ttcgcgtatg gcgaccgccc gattctgaag
     2821 aacatcaatt tcagcattcc gcaaggcaat tttgctgccg taatgggcgg ttcgggcagc
     2881 ggcaaaacca cgctgatgcg actgattaca ggacagattc gtccgcagtc cgggcaggtt
     2941 ttgattgaag gacgggattt ggcgggcttt tcggctgacg agttgtatga acaccgccgc
     3001 cgtatgggcg tattgttcca acacggcgcg ctgtttactg atttgtcggt attcgacaat
     3061 atcgcttttc cgatgcgcga actgacgcgc ctgccggaag cggtgattcg ggatttggtt
     3121 ttgttgaaat tgaacgcggt cggtctgcgc ggtgtggaaa acctgatgcc gtccgagttg
     3181 tccggcggga tgtcgcgccg cgtcgcgctt gcccgcacga ttgcgctcga ccctgaaatt
     3241 atgttgtacg acgagccgtt taccggcctc gatccgattt ccttgggcgt gattgcccac
     3301 ttgatcagcc gcgtcaacaa ggctttgcgt tcgaccagta ttatggtaac gcacgacatt
     3361 gaaaaatctt tggaaatcgt cgatcaggtg attttcttgg cgcacggcga aattatgttc
     3421 tccggctcgc cgcaggaaat gcgcgaactg gattcgcctt gggtgcgcca gtttgtcggc
     3481 gggctggcag acggccccgt agcataccgt tatccggcgc aaacgtcgtt gcagcaggat
     3541 ttgctcgggt aaattgagcc ggtgccgtct gaaaccgttg aaccttacat atagaaattt
     3601 atgaacttta tccgttccgt cggggcgaaa accctcggcc ttattcaatc cctcggcagt
     3661 atcacgctgt ttctgctgaa tattctggcg aagtccggta cggctttcgt ccgtccgcgc
     3721 ctgagcgtgc gccaagtgta ttttgccggc gtgctgtcgg tgttgattgt tgccgtttca
     3781 gggctgtttg tcggcatggt tttgggtttg cagggctata cgcagttgtc gaaattcaaa
     3841 tccgccgata ttttgggcta tatggtcgcg gcttcgctat tgcgcgaact gggtccggtg
     3901 ttggcggcga ttctgtttgc cagcagcgcg ggcggcgcga tgaccagcga aatcggtttg
     3961 atgaaaacga ccgaacagct cgaagcgatg aacgtgatgg cggtaaaccc cgtcgcccgc
     4021 gtggttgcgc cgcgcttttg ggcgggcgtg ttttccatgc cgcttttggc ttcgattttc
     4081 aacgtggcgg gtattttcgg cgcgtatttg gttggtgtaa cctggctggg cttggacagc
     4141 ggtattttct ggtcgcaaat gcagaacaac atcacgatac attacgatgt aatcaacggt
     4201 ctgatcaaat ccgcctcgtt cggcgtggcg gtaacgctga ttgccgtgca tcagggcttc
     4261 cactgcgtcc cgacctcgga aggcattttg cgcgccagca cgcgcacggt ggtttcgtcc
     4321 gccctgacga ttttggcggt cgattttata ttgaccgcgt ggatgtttac agattgacag
     4381 ggcgtctgaa gacgaaacgc ggaacatcgg atattcaagg aactttaatg aaaaagaaca
     4441 tattggaatt ttgggtcgga ctgtttgtcc tgctcggcgt ggcggcggtt gcctttctcg
     4501 ctttccgcgt ggcgggcggc gcggcgttcg gcaattccgg caagacttat accgtatatg
     4561 ccgatttcag cgacatcggc ggtttgaagg ccaatgcccc cgtcaaatcc gcaggcgtat
     4621 tggtcgggcg cgtcggcgct atcggacttg acccgaaatc ctatcaggcg agggtgcgcc
     4681 tcgatttgga cggcaagtat cagttcagca gcgacgtttc cgcgcaaatc ctgacttcgg
     4741 gacttttggg cgagcagtac atcgggctgc agcagggcgg cgacacggaa aaccttgctg
     4801 ccggcgacac catctccgta accagttctg caatggttct ggaaaacctt atcggcaaat
     4861 tcatgacgag ttttgccgag aaaaatgccg acggcggcaa tgcggaaaaa gccgccgaat
     4921 aaacgtatgc catcatattc aaactaggac acttaatcat gaaaaaatcc tccctcatca
     4981 gcgcattggg catcggtatt ttgagcatcg gcatggcatt cgccgcccct gccgacgcgg
     5041 taaaccaaat ccgtcaaaac gccactcaag tactgagcat cttaaaaagc ggtgatgcca
     5101 acaccgcccg ccaaaaagcc gaagcctatg cgattcccta tttcgatttc caacgtatga
     5161 ccgcattggc ggtcggcaac ccttggcgca ccgcgtccga cgcgcaaaaa caagcgttga
     5221 ccaaagaatt tcaaaccctg ctgatccgca cctattccgg cacgatgctg aaattaaaaa
     5281 acgccaacgt caacgtcaaa gacaatccca tcgtcaataa aggcggcaaa gaaatcatcg
     5341 tccgcgccga agtcggcgta cctgggcaaa aacccgtcaa catggacttc accacctacc
     5401 aaagcggcgg taaataccgt acctacaacg tcgccatcga aggcgcgagc ctggttaccg
     5461 tgtaccgcaa ccaattcggc gaaatcatca aagcgaaagg cgtggacgga ctgattgccg
     5521 agttgaaggc taaaaacggc agcaagtaat ccataagcca tgccgccaga ctgaagatgg
     5581 catggatttt tccctaccga acactatgca tacagaactc aacaacggaa cactgcacat
     5641 cagcggcgac gtaaccgtca aaaccctgac cgccgccgcc tttacccgct tcagacagca
     5701 atgtcgtctg aaagacaccc gcgcggttga tttgagcggc gtcggacgcg ccgattccgc
     5761 ctgcgtgtcg ctgctgctcg aagtgctgcg cggttgcaaa ggcagcgtca ggctgaccgg
     5821 cattcccgaa tccgtgcgcg cgctgtccga actgtacgaa atcaaagact ggctgaaatc
     5881 atgaaaaaaa ccgcctatgc cttcctcctg ctgatcgggt tcgcttccgc ccctgcattt
     5941 gccgaaaccc gccccgccga cccttatgaa ggctacaacc gcgccgtttt caaattcaac
     6001 gaccaagccg accgctacat tttcgcccct gccgcgcgcg gctaccgcaa agttgcgccg
     6061 aaacccgtcc gcgccggcgt gtccaatttt tttaacaacc tgtgcgacgt ggtcagcttc
     6121 ggcagcaata tcttgcgctt ggacatcaaa cgcgcaagcg aagaccttgt ccgcgtcggc
     6181 atcaacacca ctttcggttt gggcgggctt atcgacatcg ccggcgcggg cggcattccc
     6241 gacaataaaa acaccttggg cgacacgttt gcttcgtggg gatggaaaaa cagcaattat
     6301 ttcgtgttac ccgtcttagg gccgtccacc gtccgcgacg cgctcggcac gggtattacc
     6361 tccgtttatt cgcccaagaa tatcgtcttc cgcacccctg tcggacgctg gggcacgact
     6421 gccgtatccg ccgtcagtac gcgcgaaggc ctgctcgatt tgaccgacag tctggacgaa
     6481 gccgccatcg acaaatacag ctacacgcgc gacctctata tgaaagtccg tgcgcggcag
     6541 accggtgcaa cacctgccga aggtacggaa gataacatcg acatcgacga attggtcgaa
     6601 agtgccgaaa ccggcgcggc ggaaactgcc gttcaagaag attccgtatc cgaaacacag
     6661 gcagaagcag caggggaagc cgaaacgcaa cctggaacac aaccttaaag ccatgccgtc
     6721 tgaaaatccg tgttcggacg gcattccctt ctgtcggaac ggacagtccg aaaccacaga
     6781 ttgggctata atctcttttt tgcgcatacc gcgcgacagc aaaggaaaaa ccatgtacga
     6841 agtcaaccgc agcgtattcg tcctcatccc tttagaacct ttttggaact ggctgcaaac
     6901 cctgccgggc aaccacctcg acggactgac tcttgaagac atccaagccg acgccaattc
     6961 ctaccttgtc cgcccgtgcg aaaccgccga cgaagtatgg gacgaaatcg aagcccgttt
     7021 tgaagacatt ttcgccgccg aacttgccga ctggtgcgaa gacgaacgcg agtggcctgc
     7081 gctcgatgcc gacattttca acgaatggtt cgacatccag ctttctaccg tcatcaccga
     7141 cctcgaacac gaaccgcttg cccgcgaagc cttccaaccc atcaacctga actgatgaag
     7201 ctgactgtcc gcaactacca tctcgacggc tacggccatg tcaacaatgc gcgctacctc
     7261 gaatttttcg aagaagcgcg ctgggcgttt tttgaagaac gcggactgct gcacgagttg
     7321 gcaggcctca tactgattgt cgcccgcatc gacatccgat acagccgccc cgccgtcgaa
     7381 ggcgacgtat tgcagttttc atgccgcctg aaaacccccg gcacgcgccg tattgtgctg
     7441 acccagacga ttacactgcc aaacggcaaa accgccgcag aagccgacat caccctgatg
     7501 cccgtccacg ccgccacaca acgcaccgtc agcctgcccg ccacccttgc ccgcgcattg
     7561 gaagccttgt ccgaatgaaa aaaatactca ccgccgccgt cgtcgcactg atcggcatcc
     7621 tccttgccat cgtcctcatc cccgacagca aaaccgcgcc cgccttctcc ctgcccgacc
     7681 tgcacggaaa aaccgtttcc aacgccgacc tgcaaggcaa agtaaccctg attaattttt
     7741 ggtttccctc ctgtccgggt tgtgtgagcg aaatgcccaa aatcattaaa acggcaaatg
     7801 actataaaaa caaaaacttc caagtccttg ccgtcgccca gcccatcgat ccgatagaaa
     7861 gcgtccgcca atatgtcaaa gactacggtt tgccgtttac cgtcatgtat gatgcggaca
     7921 aagctgtcgg acaggcgttc ggcacacagg tttatccgac ttccgtcctt atcggcaaaa
     7981 aaggcgaaat cctcaaaact tatgtcggcg aacccgattt cggcaaactc taccaagaaa
     8041 tcgataccgc gctggcgcaa tagcgatgcc gtctgaagcg caaaaaacag aaacgatgtt
     8101 cctgtttttt cagatcatcc gccccgacac gttcagacgg cattcaacct tccggcagat
     8161 tctttttcac cactgccgga ttgcctgccg ccaaagagta gggcggaatg tcttttgtaa
     8221 ccaccgcgcc tgcgcccacg accgaaccgc gtccgacggt tacgcccgcc attacaatca
     8281 ccctgtgccc cggccagaca tcgtcctcca acgtaatcgg acggatttcc gtgtagccct
     8341 caaagcgttt tttgaatggt caaacttgtg gttatttgaa taaaacagac attccggctc
     8401 catcatcaca tttttgccga ccaccagccc acggcagatt tcacagtttg tcccgatgcc
     8461 cgagccgtcg cccaaaaccg tatccggaaa cacatacgcc ccgcgttcga tattgacccc
     8521 gcgtccgata tgcggagaaa cccgccgcgc caaaaaaccg cgcacccgtc tgccgacgat
     8581 gccgatgccg cgcatatgtg acggcggcaa ccccgaaccg gcaaaacgca gcagcaggaa
     8641 aaagaatttt gatcccatca aacctccctc aaaagaactt tttatagtgg attaacaaaa
     8701 attaggacaa ggcaacgaag ccgcagacag tacagatagt acggcaaggc gaggcaacgc
     8761 tgtactggtt tttgttaatc cactatacat tttatcgaat atactcttga cgtaacaaca
     8821 gaaaatagag agaatgtcgg acttgtctgt agcataggta catcccctat gcccgctttt
     8881 ttgaaaggaa atcaaatgaa acaaggtatt cacccgaact accacgaagt caacgttacc
     8941 tgctcttgcg gcaacaaatt cgcaaccaaa tccgcgatgg aaaaagaaaa cttcaatatc
     9001 gaggtttgct ccctgtgcca cccgttctat accggcaccc aaaaaatcgt tgataccacc
     9061 ggccgcgtgg acaaattcaa caacaaattc ggcaacctgt tcaaacgctg attgccgctt
     9121 tgcaagaatt aagccctgcc atcatttcga tggcagggct ttttaatatt tggcacactg
     9181 acttctta
//
LOCUS       DAWEWP010000076         9063 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWEWP010000076 DAWEWP010000000
VERSION     DAWEWP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9063
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.076"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..120
                     /locus_tag="WGN53_001280"
     CDS             <1..120
                     /locus_tag="WGN53_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEZ0140270.1"
                     /translation="LEEIAWRNGWLSSEEIRNLAKPMCKNEYGRYLLRLVGKD"
     gene            175..714
                     /gene="rfbC"
                     /locus_tag="WGN53_001281"
     CDS             175..714
                     /gene="rfbC"
                     /locus_tag="WGN53_001281"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0140271.1"
                     /translation="MNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERTF
                     VQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVLS
                     ADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLGE
                     PSLSAKDLAGKMLAEAATF"
     gene            876..2924
                     /locus_tag="WGN53_001282"
     CDS             876..2924
                     /locus_tag="WGN53_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ0140272.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILPHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPEINRL
                     VQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMGIYFNAEAPSRLEHILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGNSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYH
                     NLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQY
                     ADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGCFAKKLGKIKQLYRSFK
                     "
     gene            3061..4320
                     /locus_tag="WGN53_001283"
     CDS             3061..4320
                     /locus_tag="WGN53_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ0140273.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            4687..6093
                     /locus_tag="WGN53_001284"
     CDS             4687..6093
                     /locus_tag="WGN53_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0140274.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     DHLLQNGHPEQALPWLEAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAETQAHAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            6311..7525
                     /gene="gltS"
                     /locus_tag="WGN53_001285"
     CDS             6311..7525
                     /gene="gltS"
                     /locus_tag="WGN53_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ0140275.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            7624..8640
                     /locus_tag="WGN53_001286"
     CDS             7624..8640
                     /locus_tag="WGN53_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0140276.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            8651..8848
                     /locus_tag="WGN53_001287"
     CDS             8651..8848
                     /locus_tag="WGN53_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140277.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            8866..9015
                     /locus_tag="WGN53_001288"
     CDS             8866..9015
                     /locus_tag="WGN53_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140278.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFLCVSYADVPTHRTIQKAS
                     PYVPS"
BASE COUNT         2335 a         2525 c         2117 g         2086 t
ORIGIN      
        1 ttggaagaaa tcgcatggcg taatggctgg ttgagcagcg aagaaatcag aaacttggca
       61 aagccaatgt gtaaaaatga atatggccgc tatttattgc gcttggttgg caaagattaa
      121 gaggttgctt ttaccagttt tcaggccgtc tgaaacgctt tttaggaaaa catgatgaat
      181 atcatcgata cccgcattcc cgatgtcaaa ttgctcgaac ccaaagtctt cggtgacgag
      241 cgtggttttt ttatggaaac cttccgtgat gaatggttcc gccaaaatgt agccgagcgc
      301 actttcgtgc aggaaaacca cagcaaatcc acccaaggcg tattacgcgg tttacattat
      361 caaaccgaaa atacacaagg caaactggtg cgcgtggtat caggcgaagt ctttgatgtt
      421 gccgtggata tgcgtcgtga tagtcctact ttcggccact gggtcggtga ggttttatcg
      481 gcagacaaca aacgccaatt atgggtgccg gaagggtttg cccatggatt ttatgtgttg
      541 agcgatgaag cggaattcgt ctacaaatgc accgactatt acaacccgaa tgcggaacat
      601 tccctaattt ggaatgatgc cacagtggga atcgactggc ctttattggg agaaccaagc
      661 ctatcggcca aggatttggc aggcaagatg cttgctgaag cggccacatt ttaattttca
      721 cttttaggtc atcgtgggta atttgtagca agatgaccta atttttacca gctagcctac
      781 taactaaatt tctactatat ttaaatagtc agaaaattta cacaaaatct gttaataaga
      841 tattatattg tcagtaatat tttttcctat ttattgtgat tcctgcctac attcctcttc
      901 gctctttcgg tatacgtaaa attccgaata ttacgcaatt tctaccggaa tattcactta
      961 acaaaaccgt attcaacgcc caaaccatca ttggctgggg cttacgcccg accacccacc
     1021 aagcacgtgc ttttgcacaa tcacatggct tgccgtttgt agccttggaa gacggttttt
     1081 tacgctcact gggcttaggc gtggccggtt atccgccctt cgcactggtt tacgacgata
     1141 tcggcatcta ttacgacaca acccgcccat cgcgtttgga gcagttgatt ttagccgcaa
     1201 acatgccgtc tgaaatgctt aaccaagcgc agcaggccat tgatttgatt ttgccgcacc
     1261 gtttgtctaa atataaccat gctctggatt tttcagacgg cctcgtacaa gacagcaaaa
     1321 acattgtttt ggtaattgat caaactttcg gcgatatggc gattcaatat ggcggcgcag
     1381 atgaagccac attccgccaa atgtttcaga cggccttagc cgaaaatcct gatgcggaaa
     1441 tctgggtcaa aacccatccc gatgtattga gcggcaagaa acagggttac cttactgatt
     1501 tggcacagca gccgcgtgtg cgtttattgg ctgaagacat taatccgatt tcgctgctgc
     1561 aagcggtgga caaagtgtat tgcgtgacct cgcaaatggg ttttgaagcc ttgttgtgcg
     1621 gcaagccttt ggtaacgttt ggtttgccgt ggtatgccgg ctggggtgta agcgacgacc
     1681 gccatcctga aatcaaccgc cttgttcaaa cccaacgccg cgccacccgc aacttgctgc
     1741 agctcttcgc cgcagcctat ctgcaataca gccgctacct caaccccaat accggcgaag
     1801 caggcagcct ctttgatgtc atcgactatc tggcgacggt caaacgtaaa aacgacaaat
     1861 tgcgtggcga gttatattgc gtcggtatgt ctttgtggaa acgcgcggtt gccaaaccgt
     1921 tctttaacgt accctcttgc cgtctgaaat ttatctcttc cacccaaaaa ctggcaaggg
     1981 tcaaactgtc cgacgatgca cgcatcctgg cttggggcaa cggcaaagag gccatcgtcc
     2041 gctttgccga acaacaccac atccccctgc tgcgcatgga agacggcttt atccgctcgg
     2101 tcggactcgg ctccaactta gtgccgccgc tgtcgctcgt taccgacgat atgggtattt
     2161 acttcaatgc cgaagcccca tcccgccttg aacacatcct gcaaaaccaa aacttcgacg
     2221 atcaagactt tcagacggcc ttgaagttgc aaaaaatgct gaccgaaaac catatcagca
     2281 aatacaacgt cggcaactca gactttaccg ccccatcaac cgacaaaacc gtgatcctcg
     2341 ttcccggtca ggttgaggat gatgcgtcta tccgctatgg ttcgccccaa atctatcaca
     2401 atctggattt gctccgtacc gtacgcgaac gaaaccccaa tgcctatatc atctacaaac
     2461 cgcatcccga tgtagtcagc ggtaaccgca tcggccatat ttcccctgaa gatgctgcac
     2521 gatatgccga ccaaaccgcc gaacaagccg acatcctgac ctgtctccaa tacgcagacg
     2581 aaatacatac catgacttcg ctgaccggtt ttgaagcctt gttgcgcggc aaaaaagtca
     2641 gctgctacgg cctgcctttt tacgcaggct gggggcttac ccaagatctg ctccccatcc
     2701 cgcgccgtag ccgcagactt gagctttggc agctgattgc cggcacgctc atccactatc
     2761 ccgactacat ccaccccgaa acccatcagg ccataaatgc ggaaaccgca gcccaaatcc
     2821 tgatacgaca aaaaaatatg caaaaaaaca acaacggatt acatcgcggg tgctttgcca
     2881 aaaaattagg taaaatcaaa caactatatc gatctttcaa ataaatacca tcaaagttaa
     2941 cgatgcgtca taaacttgcc tctattgcgg catcattgcc tttgcatcgt taattctctt
     3001 ggcgtatgct tgaaagttca acctaaaact attacataaa aaacaaaacc acattgcaac
     3061 atgaaacaga ccgtcctcaa aaataacctg caaaacctgc ttgaaagcgc agaaaatatc
     3121 ctgctgcttc aaggccctgt cggcgatttt tttctgcgcc ttgccgactg gctgactgca
     3181 aacggcaaaa ccgtacataa attcaacttt aatgcaggcg acgactattt ttatccgccc
     3241 actcaagcgc ataccgttgt ttttaacgac aactacgatg cctttcctga gtttttgcaa
     3301 gaatacatca ctcaacatca catccaggcc gttgtctgct ttggcgacac acgcccttat
     3361 cacgtcattg caaaacgcat tgcaaacgaa aaccaagcca gtttctgggc gtttgaagaa
     3421 ggctatttcc gcccctacta catcacctta gaaaaagacg gcgtcaacgc attttccccg
     3481 ttgccgcgcc gtgccgactt ttttcttgaa caattcccta agcttgccca gcaagaatat
     3541 aaagcgccaa cgccggtaca cggcggtttt acgcccatgg caaaaaacgc tatccgttac
     3601 tatatcgagt tgttccgcaa tccacgcaaa taccccgact acatccacca ccgcgcaccc
     3661 aatgccggcc attacctcaa accgtggtcg ctctccatcc tcaagcgttt gaactactat
     3721 attgaagaca tccaaatcgc caaacgtgtg gaagcaggca aatacggcaa gttttttatt
     3781 gttcccttac aggtattcaa cgacagccaa gtccgtatcc attgcgactt tcccagcgtc
     3841 cgcagcttcc tgctccatgt tttgagttca tttgccgagc acgcgcctgc cgataccaac
     3901 atcatcatca agcatcatcc gatggaccgc ggttttatcg actactggcg cgacattaaa
     3961 cgctttatca aagaacaccc cgaactcaaa ggccgcgtga tttatgtcca tgatgtcccc
     4021 ctgcccgttt tcctgcgcca cggtctcggc atggtcacca tcaacagcac cagcggcctg
     4081 tccggactga ttcacaatat gccagttaag gttctcggcc gtgcctatta tgatattccc
     4141 ggcattactg accaaaatac cttggcagaa ttttggaatc atccgacacc gcctgacaaa
     4201 gagctgttcc atgcctaccg aatgtaccac ctcaacgtga cccaaattaa cggcaacttc
     4261 tacagtcagg tgtttttccc caacaaaaaa acctccaact cttccacacc agtaatctga
     4321 cttagcgaag gaagttcagg ccgtctgaaa acatttcaga cggcctgaaa caatcaatac
     4381 cttagctact gccatgtaaa taaaacacaa aaatctgcat ttatcattaa caataaatta
     4441 caaaaacagt ataatgaccg agctgccatg agcgcatacc gactcaacct gagcccttta
     4501 taacacacaa aatatggatg tatccccagg caaaacaata taacaagcca aacatcctaa
     4561 agataagccg gcaaggaaat acactctgca aaacgatgcc gagcaatatt tttacaaagc
     4621 cctcaaccgg tatcgccgcc catatgccgc agcatccgtc ttccacttta tatccgcccg
     4681 caaaccatga ccgccgctcc tgatatccta taccgtcaag ccgccgccct tttggaacaa
     4741 tccaataccg cccaagccct gcccctgctg caacaggccg cagagcaggg atatacggaa
     4801 gctgcttttg tattgggcga ccatctgctg caaaacggtc atccggagca ggcacttcca
     4861 tggttggaag ccgccgcggc ccaacgccat cccaaggcac tctttaccct gctgcaacaa
     4921 cgcgaacaca acggcacccc gaccggacag cttcttaacg aatacgcctg gctgagtgag
     4981 caaggtcacc tcgaagccca attaattctc atgcgttacc acacgcagcg caacgatcca
     5041 caatcgctct actgggcgga gcttgccgct gcccaatatg ccgcacctgc gtattaccat
     5101 ttggcacgcc atcatcaacg ccaaggcgac gttgcaacag ctatcgaaca atacgaaaaa
     5161 gcggcagcgc tcggcgtgac tgcagcctgc tggcaactcg gtcaaatcta cttctacggc
     5221 acaggtgtca gccccaacca cgcacaagcc gaacactatc tcgaacaagc cgcgcaagcc
     5281 ggccacatcg ccgcacaaac cctgctggcc gaccttcttg ctgcccaacg caaacccgaa
     5341 gccttagaat ggtatcgtcg tgccgccgac aaagagcaag cggaagcaca ggccaagctg
     5401 gctcaatacg ccctgaccgg cgaactttcc gaacgcgatc cgtttcaagc ggcacgatat
     5461 gccaaagccg ccgctgagaa agaccatccc gaagccctga aaatcatggg cgacctctac
     5521 cgctacggcc tcggcatcaa agctgacaac catatcgcgc acgattacta ccaccgtgct
     5581 gccgcgctgg gttctgccgc cgcagcacaa aaactcatca gcgacgccgc gctgtaccat
     5641 ccgcaacaat acgaacaaat caaaactgcc gccctgcaac agcaacaaac cgaaaccatc
     5701 taccgtttgg cggaaacaca agcccatgcc atcggtcgtc ccgccgacta taatgccgca
     5761 cgcaaaaatt acatggaagc tgccgggttc caccataaaa acgcggcggc tgccttaggc
     5821 cgcatctacc attacggcct cggtacggcg caagatcctc gggcggctgc acactggtac
     5881 gccattgctg ccgaacaaaa ccacccttcc gcccaatacc acctcgcctg tttttactat
     5941 cacgggcaag gtgtcggctg tcatgttccg accgcctgct actggctgca ggccgccatc
     6001 ggcaacggcc acacttcggc cgaatcatta atatccctat tagaacaatg gcgacgcgaa
     6061 gcacaccatg ccatcggaca aaaggccgtc tgaaaagatt tacactcgca ttttttgaca
     6121 atctttaact attcccctaa tatttgccag ttatttttca cggacacgcc attgttttca
     6181 tttctttctg aaaacacctt gtccgcgcat caataccatg acactcggcg gataacgcca
     6241 agcgttgaaa cacactacat ccggaacaaa aacggatgct cggaaaaata tttctaggag
     6301 gtgaaacaac atggaatggg aattcaacag ttattacaca ctgattgccg ccacgctcgt
     6361 gttgctggtt ggtaaatttc tggttcaaaa aatcaaattc ttacgagact tcaatattcc
     6421 cgagccggtc gctggcggtt tggttgccgc gattatcctg tttgcgctgc acgaggcgta
     6481 cggcgtgagc ttcaaatttg agaaaccgct gcaaaatgcg tttatgctga tttttttcac
     6541 gtccatcggc ttgagcgcgg atttttcccg tttgaaggcg ggcggtttgc cgctggtggt
     6601 ttttaccgcg attgtgggcg gatttatctt ggtgcaaaac tttgtcgggg tcggactggc
     6661 tacggctttg ggtttggacc cgctcatcgg tctgattacc ggttcggtgt cgctgacggg
     6721 cggacacggt acgtcaggtg cgtggggacc taattttgaa acgcaatatg gcttggtcgg
     6781 cgcaaccggt ttgggtattg catcggctac tttcgggctg gtgttcggcg gcctgatcgg
     6841 cgggccggtt gcgcgccgcc tgatcaacaa aatgggccgc aaaccggttg aaaacaaaaa
     6901 acaggatcag gacgacaacg cggacgacgt gttcgagcag gcaaaacgca cccgcctgat
     6961 tacggcggaa tctgccgttg aaacgcttgc catgtttgcc gcgtgtttgg cgtttgccga
     7021 gattatggac ggcttcgaca aagaatacct gttcgacctg cccaaattcg tgtggtgtct
     7081 gtttggcggc gtggtcatcc gcaacatcct cactgccgca ttcaaggtca atatgttcga
     7141 ccgcgccatc gatgtgttcg gcaatgcttc gctttcgctt ttcttggcaa tggcgttgct
     7201 gaatttgaaa ctgtgggagc tgaccggttt ggcggggcct gtaaccgtga ttcttgccgt
     7261 acaaaccgtg gtgatggttt tgtacgcgac ttttgttacc tatgtcttta tggggcgcga
     7321 ttatgatgcg gcagtattgg ctgccggcca ttgcggtttc ggcttgggtg caacgccgac
     7381 ggcggtggca aatatgcagt ccgtcacgca tactttcggc gcgtcgcata aggcgttttt
     7441 gattgtgcct atggtcggcg cgttcttcgt cgatttgatt aatgccgcga ttctcaccgg
     7501 ttttgtgaat ttctttaaag gctgattttc cgcctttccg ccaaagcacc tgcaaggttt
     7561 accgcctgca ggtgcttttg ctatgatagc cgctatcggt ctgcaccgtt tggaaggaac
     7621 atcatgtatc ggaaactcat tgcgctgccg tttgccctgc tgcttgccgc ttgcggcagg
     7681 gaagaaccgc ccaaggcatt ggaatgcgcc aaccccgccg tgttgcaagg catacgcggc
     7741 aatattcagg aaacgctcac gcaggaagcg cgttctttcg cgcgcgaaga cggcaggcag
     7801 tttgtcgatg ccgacaaaat tatcgccgcc gcctacggtt tggcgttttc tttggaacac
     7861 gcttcggaaa cgcaggaagg cgggcgcacg ttctgtatcg ccgatttgaa cattaccgtg
     7921 ccgtctgaaa cgcttgccga tgccaaggca aacagccccc tgctgtacgg ggaaaccgct
     7981 ttgtcggata ttgtgcggca gaagacgggc ggcaatgtcg agtttaaaga cggcgtattg
     8041 acggcagccg tccgcttcct gcccgtcaaa gacggtcaga cggcatttgt cgacaacacg
     8101 gtcggtatgg cggcgcaaac gctgtctgcc gcgctgctgc cttacggcgt gaagagcatc
     8161 gtgatgatag acggcaaggc ggtgaaaaaa gaagacgcgg tcaggatttt gagcggaaaa
     8221 gcccgtgaag aagaaccgtc caaacccacg cccgaagaca ttttggaaca caatgccgcc
     8281 ggcggcgatg cgggcgtacc ccaagccgca gaaggcgcgc ccgaaccgga aatcctgcat
     8341 cctgacgacg gcgagcgtgc cgataccgtt accgtatcac ggggcgaagt ggaagaggcg
     8401 cgcgtacaaa accagcgtgc ggaatccgaa attaccaaac tttggggagg actcgatacc
     8461 gacgtgcaaa aagagttggt cggcgaacaa cgcaagtggg cgcaggaaaa aatcagcaac
     8521 tgccgacaag ccgccgcgca ggcagaccgg caggaatacg ccgaatacct caagctgcaa
     8581 tgcgacacgc ggatgacgcg cgaacggata cagtatcttc gcggctattc catcgattag
     8641 gggcaaaccg atgactaccg tccccaaaag caggattccc gtcaaaccgc tgcccgaaaa
     8701 aaccacagac gaagccaaag tcgaaaaatg gcggcagctc ggtgcggaac acggtttgtc
     8761 gggcgaatgg gcagttgccg ccagattggg cgagaacggt tttaccgaag aacagatgga
     8821 aaatatcgcc aacctgttcg gcagataaag ggaaaattga cggaaatgcc gtctgaaacc
     8881 ctgttatcgg tttcagacgg cattttgact aatacggtac gcaggcgcaa aacagccggc
     8941 tttttatgtg tttcctatgc tgatgttcca acacacagga cgatacaaaa agcgtcgccc
     9001 tatgtgccgt cctgattcgg aaagggttac gctcctccca aatatagtgg attaacaaaa
     9061 acc
//
LOCUS       DAWEWP010000077         8922 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWEWP010000077 DAWEWP010000000
VERSION     DAWEWP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.077"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            188..622
                     /locus_tag="WGN53_001289"
     CDS             188..622
                     /locus_tag="WGN53_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0140279.1"
                     /translation="MNFALSVITFTLASFLPVPPAEAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(671..2014)
                     /locus_tag="WGN53_001290"
     CDS             complement(671..2014)
                     /locus_tag="WGN53_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ0140280.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLVSDGIPLAGAWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(2019..2672)
                     /locus_tag="WGN53_001291"
     CDS             complement(2019..2672)
                     /locus_tag="WGN53_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ0140281.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIVAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2823..3113
                     /gene="gatC"
                     /locus_tag="WGN53_001292"
     CDS             2823..3113
                     /gene="gatC"
                     /locus_tag="WGN53_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ0140282.1"
                     /translation="MALTLADVDKIARLSRLHLTAAEKEKSLQELNDIFAMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            3176..4621
                     /gene="gatA"
                     /locus_tag="WGN53_001293"
     CDS             3176..4621
                     /gene="gatA"
                     /locus_tag="WGN53_001293"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ0140283.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAITGFDPKDSTSLEREKEDYTRDLNQPLKGVKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4618..5550
                     /locus_tag="WGN53_001294"
     CDS             4618..5550
                     /locus_tag="WGN53_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ0140284.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5593..7023
                     /gene="gatB"
                     /locus_tag="WGN53_001295"
     CDS             5593..7023
                     /gene="gatB"
                     /locus_tag="WGN53_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ0140285.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPELR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGSKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ASGQGKPTANWMNGELAAALNKAGLELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPESTIAEIIEKHGLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7115..8125
                     /locus_tag="WGN53_001296"
     CDS             7115..8125
                     /locus_tag="WGN53_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ0140286.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQIGGHSEQALSEAEKAQGKILMCCTTAQSDISLNIPGYKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSGRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8254..8886)
                     /gene="pdxH"
                     /locus_tag="WGN53_001297"
     CDS             complement(8254..8886)
                     /gene="pdxH"
                     /locus_tag="WGN53_001297"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ0140287.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
BASE COUNT         2278 a         2744 c         2171 g         1729 t
ORIGIN      
        1 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatattttt gggaatctgt
       61 tttaccccaa tatataaagc accatattaa ggcggagttt cttccccact ttgaaccgaa
      121 cccggaaaag acaccgccca agccaatcct gatgctgccc cggcagccaa ccatcaagga
      181 aaccctaatg aactttgctt tatccgtcat cacatttacc ctcgcctctt tcctgcccgt
      241 cccgcccgcc gaagccgccg tctttacttg gaaggacggc ggcggcaaca gctattcgga
      301 tgtgccgaaa cagcttcatc ccgaccagag ccaaatcctc aacctgcgga cgcgccaaac
      361 caaaccggcg gtcaaacccg cccaagccga cgcagggaag cgcacagacg gcgcggcaca
      421 ggaaaacaat cccgacactg ccgagaaaaa ccggcagctt gaggaagaaa agaaaagaat
      481 tgccgaaacc gaacggcaga acaaagaaga aaactgccgg atttcaaaaa tgaacctgaa
      541 agcggtggga aattcaaatg caaaaaacaa ggatgatttg attcggaaat acaataacgc
      601 cgtaaacaaa tactgccgtt aatcggctct agcgcaaacc ccatgccgtc tgaaacggca
      661 cggggtttgt ttatttctgc cagtaggttt tgacgttgac gaactcgtac agcccgaatt
      721 cggacaattc gcgcccgtaa ccggaatctt tgactccgcc gaaaggcagg cgcaaatcgc
      781 tgctggtatg gcggttgata aacaccgatc ccgcctgtat tttttcggca aaccgccaag
      841 cgcgttcggt atcggcggta taaatgcagg cacctagccc gaacggggaa tcattggcaa
      901 gggcgatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc ccgaatactt
      961 cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg ggataaaacc
     1021 agcctcgccc ttgtgggatt tttccgccgg tcaggcatac cgcgccgttt gaaacggcat
     1081 cttcaacctg cccgtgaacc ctgtcccgca aatcttcgcg gtgcagcggt gcaagcgtag
     1141 tatcgggatg tttggggtcg cccattttca atttggcgca ttcggcaaga aacagcgtga
     1201 taaaacgatc ggctgcggct tcggttacga tgatgcgttt ggcggcgttg cacgattgcc
     1261 ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc gcgttccaaa tcggcatcgg
     1321 gcatcacgat aaaggcgttg ctgccgccga gttccaagac ggttttcttg aggtttgcgc
     1381 ccgcatgtgc cgcaaggatg cgccccgtat gcgtcgagcc ggtaaacgcc atcgcatcgg
     1441 tatcttcaac cgccttgagc gtgcccgcct catccaacca cgcgcctgcc aaaggaatgc
     1501 cgtctgaaac caaatcaaac aacgcctgac tgacgcgtgc cacgctgggc gcgggtttga
     1561 cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc tgccagacgg
     1621 gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcaaacctt acctgactca
     1681 aactcgcctg cgtcgcgatg gttttgtggg cgagcagttc gggagcgagg cgggcgtaat
     1741 agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcagg cagcgtccga
     1801 cttcttcgca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg tcggcaaatt
     1861 tttgcagacg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg ccgcgcattt
     1921 tcaaatccgc cagccgccgt tcaaactccg cataatcttg agctggacgg cggtaaagcg
     1981 tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag ttgcgggaat
     2041 atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat catacccatg
     2101 atacggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt ccctgcaaca
     2161 attaaaatgg cataacaaat cgcactgacc accaaaccgg ccacgataat cagcgcgatg
     2221 tcgctgtacg ttttggctgc cgaagcgtaa ataatcacgg tcgaaatacc gcccgggccg
     2281 atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg ggcggggcgc
     2341 gcctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc gttgccgttc
     2401 atcatcgaaa tggcgatcag cagcaccaaa atcccgccgc cgacctgaaa cgaaccgacg
     2461 ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc aatcacggca
     2521 aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct gtgcccgttg
     2581 gtcaggtcaa ggtaaagcga caacgcgcta aacggattaa tcagcaccaa aaaagccaca
     2641 atcagcttgc cgatttccat gcccaatccc attatttccc cctccttcaa acccgtgcgg
     2701 caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa ttaaaaattc
     2761 aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca aggatgcttt
     2821 tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga ctgcacctga
     2881 ctgcggcaga aaaagaaaaa tcgcttcaag aattaaacga cattttcgct atggtcgaac
     2941 agatgcaaac catcaacaca gacggcatcg aaccgatggc gcatccgcac gaggccgccc
     3001 tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat caggcggttg
     3061 ctccggaagt acgcaaccgt ctgtatatcg taccgcaagt tatcgaagaa taatccgaat
     3121 atgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa gatttatgac
     3181 ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt ccgccgtcga
     3241 actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca acggctacat
     3301 caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac gtatcgcgca
     3361 gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt tctgccaaac
     3421 cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc cttacaccgc
     3481 caccgtcgtc caaaacctgc tcgacgaagg catggtaacg ctcggccgca ccaatatgga
     3541 tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggcgcggcca aaaatccgtg
     3601 gaatccggaa cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg ttgccgcgcg
     3661 ccttgcccct gccgcgctcg gttcggatac cggcggctct atccgccagc ctgcatcgca
     3721 ctgcggcatt accggcatca aacctaccta cggcacggtt tcccgcttcg gtatggtcgc
     3781 ctacgcctcc agcttcgacc aggccggccc gatggcgcaa actgccgaag actgcgcgat
     3841 tctgttgaat gcaataacag gtttcgaccc caaagactcc accagtctcg aacgtgaaaa
     3901 agaagactac acccgcgatt tgaaccaacc gctcaaaggc gtgaaaatcg gtctgcccaa
     3961 agaatatttc ggcgaaggca acagcgccga tgttctgacg gcattgcaaa acaccattga
     4021 tttgctgaaa gcccaaggcg cggaattgat tgaagtttcc ctgccgcaaa ccaagctgtc
     4081 catccccgcc tactacgtcc tcgcctccgc agaagccggc accaaccttt cacgttacga
     4141 cggcgtacgt tacggacacc gtgccgccca attcgccgat ttggaagaaa tgtacggcaa
     4201 aacccgcgcc gaaggtttcg gcagcgaagt caaacgccgc atcatgatcg gcacttatgt
     4261 actgtcgcac ggctactacg atgcctacta tctcaaagcc caaaaactgc gccgcctcgt
     4321 tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc cgaccgcacc
     4381 cactgcagcc ccaaaaatag gagcggatgc ttcgccggtt gaaacctact tgagcgatat
     4441 ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg caggcttcag
     4501 cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg aagccaaaat
     4561 cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac gacccgaatg
     4621 aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta tgcgccttta
     4681 tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa agaaatttac
     4741 gccttcgagc agtttgtcaa cgcctcccct atccgtcagg ggctgttcct ccactgcccg
     4801 caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg caaacgccgt
     4861 ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac agacatcctg
     4921 caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga cggcatcagc
     4981 ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt atctttgcgc
     5041 gacgaagcag gcaaccggct gtatatggcg actttcgcct ttgtcggcac acacctgctg
     5101 acagcctccg tacaagggcc tgcgggtgaa gaagccaaag acactgtccg ccgcataacc
     5161 aaacaacttc acggcttgcg tccccaacaa ctgatggtaa ccgcactgca atatttcgcc
     5221 gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt caaactgcgc
     5281 tggaaactta aaaagcgcgt caaaatgaat tacgacgcat tctggcagga atacggcgca
     5341 agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa agaccttacc
     5401 gacatcgaaa gcaaaaaacg ctcgatgtac cgcaagcgtt atgaaatgct ggacaacatg
     5461 gttgcagaga tgaaggacgg tctgaaaaca gaagcacgcg gcatttcaga cggcatccaa
     5521 acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga tattttagag
     5581 aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt ccaattgaac
     5641 accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc caacgcgcac
     5701 gccagcgtag tggaatgcgc gctgccgggc gtactgccgg taatgaaccg agaagtcgtt
     5761 gaaaaagcca tcaaattagg cttggctttg gatgcgaaaa tcaaccggaa aaacgtgttc
     5821 gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag ccagttggat
     5881 ttgccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt gaaaaccatc
     5941 aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtgcatga aggcttgaac
     6001 ggcgcgaccg gtatcgacct gaatcgcgcc ggcacgcctt tattggaagt cgtatccgaa
     6061 ccggaattgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca cagcttggta
     6121 acctggctgg acatttgcga cggcaatatg gcggaaggtt cgttccgcgt cgatgccaac
     6181 gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat taaaaacctc
     6241 aattccttcc gtttcttgga gcaggcgatt aattacgaag cggaagcgca aatcgagatt
     6301 ttggaagacg gcagcaaagt gcagcaggcg acgatgctgt tcgacccaga aaaaggcgaa
     6361 acccgcgtaa tgcgcctgaa agaagacgcg caagactacc gctacttccc cgacccggat
     6421 ttgttgcccg tcatcatttc agacgctcaa atgcaaaaag ccaaagcaga aatgcccgag
     6481 ctgccgaaag aaatggcggc acgtttcgtg gcagattacg gcgtgtccga atacgacgcg
     6541 cgcctgctca ccgcaagccg cgcacaggcc gcctattttg aagaagccgc caaagcaagc
     6601 gggcaaggca agccgactgc caactggatg aacggcgaac tcgccgccgc gctgaacaaa
     6661 gccggcctgg aacttgccga cagcccgatt accgccccgc gcctcgccgc gctggtgggc
     6721 aaaatcgccg acggcacatt aagcagcaag ttagcgaaaa aagcctttga agccatgtgg
     6781 gcagaacccg aatccaccat tgccgaaatc attgaaaaac acggcttgca gcaaatgacc
     6841 gataccggcg cagttgaagc gatggtggac gaagtgctgg caaacaacgc caaagccgtg
     6901 gaacagttta aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca agtgatgaaa
     6961 gccagcaaag gcaaagccaa ccccgcgcaa gttcaagaac tgattaaagc caaacttgcc
     7021 tgataagcaa gatgccgtct gaaacccaga tccgacgttc agacggcgtt ttcacagtct
     7081 gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccgtta ccctgcccga
     7141 ccaaaccacc tttgccgccg gcgacggtga aaccgttttg gccgctgccg cccgtcaaaa
     7201 cctcaatctg ccccattcct gcaaaagcgg tgtctgcggg caatgcaaag ccgaactggt
     7261 cagcggcgat attcaaatag gcggacactc ggaacaggct ttatccgaag cagaaaaagc
     7321 gcaaggcaag attttgatgt gctgcaccac tgcgcaaagc gacatcagcc tcaacatccc
     7381 cggctacaaa gccgatgccc tacccgtccg caccctgccc gcacgcatcg aaagtatggt
     7441 tttcaaacac gatgtcgccc tcttgaaact tgccctgccc aaagcccctc cgtttgcctt
     7501 ctatgctggg caatacatcg atttactgct gccgggcaac gtcagccgca gctactccat
     7561 cgccaattcg cccgaccaag aaggcatttt ggaactacac atccgcaggc gcgaaaacgg
     7621 tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag gcatcgtccg
     7681 cgttaaaggc ccgctcggtt cgtttaccct gcaggaagac agcggcaaac ccgtcatcct
     7741 gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc ttatccgcca
     7801 agacagcggc cgcgccgtcc atttctactg gggcgcgcgt catcaagatg atttgtatgc
     7861 cctcgaagaa gcacaagggt tggcatgccg tctgaaaaac gcctgcttca cccccgtatt
     7921 gtcccgcccc ggagagggct ggcagggaag aaaagggcac gtacaagaca tcgcggcaca
     7981 agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggctctccgg ccatgaccga
     8041 acaagcaaaa aatctgtttg tgcaacagca taagctgccg gaaaacttgt ttttctccga
     8101 cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg ctttccgttc
     8161 ggaacacaaa aaacttcccg tccgtatttt ccccataaaa aatgccgtct gaagcccgat
     8221 tccggttttc agacggcata tgttttttcc tgttcaaggc gacagccgct cgcgtatcca
     8281 gccaccatcc agcaaacggt attggatgcg gtcgtgcagc ctgctcggcc tgccctgcca
     8341 aaactcaatc aaatcgggaa tcacaatata gccgccccaa tgcggcggac gcggcacgtg
     8401 caaaggatgt ttgagtccga ccgccgccgc ctttgccacc aataccgcct tgttcggaat
     8461 cacctcgctc tgcgcacttg cccacgcacc caaacggctc tgatacgggc ggctctcaaa
     8521 atattcgtcc gacaacttct ccgccagcct ttcaacacgc ccttccacgc gcacctgacg
     8581 ctccagctcc ggccaaaaaa acgtcatcgc cgcaaatgga tgagcatcca gcgaacgccc
     8641 cttgcggctg tgataattcg taaagaaaac aaaaccttca gaattaactt ccttcagcag
     8701 caccatacgg ctgttaggcc tgccgcgtcc gtcgaccgcc gccacattga ccgccgtcgg
     8761 ctcgttgacc tgtgcgcgta ccgcctcgtc caaccaccgc tcgaactgct cgatcggatt
     8821 gtcggcgcaa tcgccttccg acaattcccg tttgctgtaa tcttcccgaa tattgtgcaa
     8881 atccatttac cgctcctctt aatagtggat taacaaaaac ca
//
LOCUS       DAWEWP010000078         8824 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWEWP010000078 DAWEWP010000000
VERSION     DAWEWP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.078"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            179..1354
                     /locus_tag="WGN53_001298"
     CDS             179..1354
                     /locus_tag="WGN53_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ0140288.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(1565..2113)
                     /locus_tag="WGN53_001299"
     CDS             complement(1565..2113)
                     /locus_tag="WGN53_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ0140289.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WGN53_001300"
     CDS             complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WGN53_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ0140290.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            4140..6119
                     /gene="tkt"
                     /locus_tag="WGN53_001301"
     CDS             4140..6119
                     /gene="tkt"
                     /locus_tag="WGN53_001301"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ0140291.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEKGAKLEAEWDKLFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DGYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIINGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDTAYQAAVLPEGLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKEFGFTVDNVVDTVKSVL"
     gene            6267..7655
                     /gene="fumC"
                     /locus_tag="WGN53_001302"
     CDS             6267..7655
                     /gene="fumC"
                     /locus_tag="WGN53_001302"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ0140292.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(7720..8643)
                     /locus_tag="WGN53_001303"
     CDS             complement(7720..8643)
                     /locus_tag="WGN53_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0140293.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAKPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
BASE COUNT         2230 a         2537 c         2161 g         1896 t
ORIGIN      
        1 cactataata ttttctctcc cgattgaaac aggcgtaaca gaatatgccc gaagctccgg
       61 ctgctttctt gtttaccgcc gcgatattta gagtataata ccaaatttga gcaatagttc
      121 taaaacagtt agaaccattt ttcatgagcc tgactgattc gtacactcgg agaaactgat
      181 gcagaatatt tttgaccctt tggttattcg tggaaaatcc cttaccccca tcgtgcaagg
      241 cggtatgggg gtcggtgttt ccgcatcggg tttatccagc gcggtggcgc gtgaaaacgg
      301 tatcggaacg attgccagtg tggatttgcg ccaccttcac gaagacctac tcgccgaatc
      361 acaaatcaat ccgagtgaag agaaatatac atctttgaac tgtaccgcat tagacaggga
      421 aatccaaaaa gccaaaagcg cttcagaggg aaaaggactg attgcggtca acgtgatgaa
      481 ggcggtcaaa gaccacgccg cgtatgtccg ccaggcttgc gaatcagggg cggatgcggt
      541 tgtaatgggt gccggcctgc ctttagacct gccggaaatg accgagggct atcataaaga
      601 tgtcgcgctg ctgccgattc tgtccgaatc gcgcggtatt aatatcgtct tgaaacgttg
      661 gatgaaaaaa ggcatattgc ccgatgcgat tgtagtcgaa catcctgccc acgcggccgg
      721 acatttgggt gcatcaaccg ttgaaggcgt aaacgatgcc aagttcgact tcaaacgcgt
      781 gattgaggaa acgtttgaag ttttcaaaag tttagggctg gaaagcgaaa aaatcccgct
      841 tattcttgcg ggaggcatgg caaattttga aaaagtcaaa accgccctaa agaactgggg
      901 agcatccgcc gttcaaatcg gtacggcttt tgccgttacc gaagaaggag atgcacacct
      961 taacttcaaa aaaacgctcg ccggtgcgga aactgaaaaa gtagtcgaat ttatgtctgt
     1021 tgccggtttg ccggcgcgcg gtgtccgcac caaattccta gacagctaca tcaagcgtga
     1081 aagcaaactt cagacaaacg ccaaagccga cccgcgccgc tgtacccaag gtttaaactg
     1141 cctaaccagt tgcggtctgc gcgacgggct ttccaaagca ggacagttct gtattgatat
     1201 ccagcttgcc gccgcattcc gtggagaagt agataaaggc ctgttcttca gaggtaaaga
     1261 cccgctgccc ttcggcaatg ccatccgcac cgtccgcgag acgatacaat atctgctgac
     1321 ggggagcgaa cctgttgcaa cgctcggacg ctgacatcag gtaggatttg atttgcaaac
     1381 aaaatgccgt ctgaagggct ttcagacggc gtttttcagg ctgcgttccg gaatagtgtt
     1441 aaaaaataaa cgggatgaga tacatttatt ttcgtccgac aaatcaaacc atcgcgccga
     1501 atgatcaaat aatgcctgca cggcattaca tctggcaaag caatgcaatg aaaacacggc
     1561 ttttttattt gctttcagta ttattgaaaa gcttgtccat cggggtcaaa tcgaccgcat
     1621 tgccttggct ggtaatccat ttgttctcga cgcgccacac gcctgccgcg tcctccacgc
     1681 gcacatacca gtggttggtc ggcgaaaggg ttttgaacac cgcctcatat tccgccctgc
     1741 cgttctgcgc gctgccgacg ggcttgaggg cgacggtttg atcgtccgcc ttgcgggtcg
     1801 ggtgcatcag cagcaggttc aaaggctgtt tgccgtcaaa ctcgccgccg acaaacactt
     1861 ttgccgcatt catatcgggg gaaatgagaa cctgcacccc gatatgccgt ctgacggctt
     1921 cttcatcccg atgaagctgg atgtcgatat gtttgccgtc tttataataa tcgtccgtaa
     1981 ccaaatctgt cgcgtgctgc tgcgcgacaa aaaacatagc gacgctggcg atgacgacaa
     2041 aaatcggccc cgccatcaag atccacggcc agacgtgttt gtaccaaggt ttgattggag
     2101 tgttttgaga cacggttatt ctccgataaa ggttgcatct tcttccaaga cgaccggctt
     2161 gccgtcgggc gcgccgcttt cgcggtattg gaaggtaaat tcgatagggt ggctgccttt
     2221 gtccgcgtat tccggaatgg tggacacttg gacgggaagg gttaccgttt cgcgcggggc
     2281 aaccttgata ccgccttcgg gcagcccggt cagggcgatt tcgtcaaagc ctttgacact
     2341 tgcggtaatg agctgttctt tttcactttt gttgatgata cgcaggctgt atgcgttttc
     2401 cagccagcct ttggcgtttt cgcgcaccag tacgccacgg tctttcaaaa tatcgacctc
     2461 gaccattttg cgcgtggaca aaccggccag gaaggcaatg ataactaacg ccaacaccgc
     2521 gccgtaacct gccacgcgcg gtctgagcag ccgtttttta atgtcttttt cagaatattc
     2581 gtgttccagc gcgctttcgg tcgtataacg gattaatccg cgcggatagc ccattttgtc
     2641 cataatctca tcgcacgcgt cgatacaggc ggcgcagccg atacattggt attgcagacc
     2701 gttgcggatg tcgatgccga cggggcagac ttggacgcac atcgcacagt tgatgcagtc
     2761 gcccaaaccc gcctcttcct tattgaccgt tttcttgcgc gcgccgcgcg gttcgccgcg
     2821 ttccgcgtca taagaaacaa tcagcgtgtc cttgtcgaac atcgcgcttt ggaaacgtgc
     2881 atacggacac atatgcaggc atactttttc acgcataatg tgggcgaaga agaaggtcat
     2941 aaagccataa aacgctgcgg caaacatcgc gccgccacct gctgctccag tgaataaatc
     3001 gggaacgaac tggcggatag ggacaaacca gcctgcaaac gtgatgcccg tccacgcgca
     3061 gacaaggaaa atcagcaggt atttggtggc tttgatgcgg attttagtga aattccacgg
     3121 cgatttttcc agtttcagcc gtttgtttct atcgccttcg accaggttgt caatccacag
     3181 cataatttcg gtgtaaaccg tttgcgggca ggaatagccg caccacagtc gccctgcaat
     3241 cgtcgtccac caaaacagcc cgaaggcgca aatcatcagc agcaaggcaa ggtaaatcaa
     3301 atcgcccacc cccaacgaca atccgaaaat gaagaaatgc cgttcgggga tattgaaaac
     3361 gacggcctgc ctgccgctcc agttgaacca cggaatgacg taaaacacaa actgcgtcgc
     3421 caatacggcg gcgatacgca gtttggcgaa ccgtccttcc gcctttttgg gatggatgcg
     3481 ttcgccttcg ggatggattt gaatcacgct ggctcgcgga tcgaatgttt tttttgcttt
     3541 cggcgcggct tttgtttgtt cggacgtgcc gattccggat gccggactgc cggcttggtt
     3601 ttccgtggtc attctgcatt ccttagattt ttgattgatg gtttgcccgt taccgccgcc
     3661 gtttgctttt cagacgtcat ttttcttgtt ttttaaggcg ttgtgtttca agttttgaga
     3721 aaatccgttt ttcccaaaat atatttccgc tattgtacaa ctttatgcgc cgtccggatg
     3781 tatggggcgg atacatttcc catccgcatc aaaacgcctg gattttacct taccgcccga
     3841 acaaaatccg aatacggtta aaaaaaaaga ctaaaaaaac cgacaccccc atatcggcag
     3901 aaccgacggc gcaagctcat aaacaaacgc tatcgacaat ccggcacaca atctataact
     3961 ttttatttca aaaggaataa tggcaggctt cgcccgcaaa tcgaaaatcc ttccccgcct
     4021 gtcccctgcc gccgccttcc cacgcgtccg cccttttctt gaaagcataa gcgaatcggg
     4081 cgataatcaa cgctttccga ttatccactt atctgaaaca ccagcaagga aaatacaaaa
     4141 tgtctcaact ggcaaacgca atccgcttcc tctcggccga tgccgttcaa aaagccaatt
     4201 ccggccaccc cggcgcgcct atgggtatgg cggaaatggc ggaaacattg tggacgaaat
     4261 tcctcaatca caaccccgcc aaccccaaat tctacaaccg cgaccgcttc gtcctctcca
     4321 acggccacgc gtctatgctg ttgtacagcc tgctgcacct gaccggctac aacctaagca
     4381 ttgaagactt gaaaaacttc cgccaactgc acagcaaaac ccccggccat cccgaatacg
     4441 gctacaccga cggcgtggaa accacgaccg gcccgttggg gcaagggatt gccaacgcgg
     4501 tgggtatggc attggcagaa aaaatccttg ccgccgaatt taataaagac ggtttgaaca
     4561 tcgtcgatca ttacacctac gtctttatgg gcgacggctg tctgatggaa ggcgtatcgc
     4621 acgaagcctg ttcgctcgcc ggcaccttgg gcttgggcaa actgattgtt ttatatgatg
     4681 acaacaatat ttccattgat ggtaaagtgg acggctggtt taccgaaaac atcccgcaac
     4741 gctttgaaag ctacggctgg cacgtcgttc ctaacgtaaa tggtcatgac accgccgcca
     4801 ttcaagccgc catcgaagcc gcacgtgccg aaaccggcaa accgtccatc atctgctgta
     4861 aaaccttaat cggcaaaggc agtgccaata aagaaggcag ccacaaaacc cacggcgcac
     4921 ctttgggtgc ggacgaaatc gaagccacgc gcaaacattt gggctggact taccctgcct
     4981 ttgaaatccc gcaagaaatt tacgatgcgt ggaatgccaa agaaaaaggc gcgaaactgg
     5041 aagccgaatg ggacaaactg ttcgcgcaat accaagccaa atatcctgcc gaagccgcag
     5101 aatttgtgcg ccgtatggat aaaaaactgc cggaaaactt tgacgggtac gttcagacgg
     5161 cattgaaaga agtgtgcgcc aaagccgaaa ccgtcgccac ccgcaaagcc agccaaaaca
     5221 gcatcgaaat cttggcaaaa gagctgcccg aactggtggg cggctctgcc gacctgaccc
     5281 cgtccaatct gaccgactgg tcaaacagcg tctccgttac ccgcgacaaa ggcggcaact
     5341 acatccacta cggcgtgcgc gagttcggca tgggtgcgat tataaacggc ttggtattgc
     5401 acggcggcgt gaaacccttt ggcgcgacct tcctgatgtt cagcgaatac gagcgcaacg
     5461 ccctgcgtat ggcggcactg atgaaaatca accccgtgtt cgtgttcacc cacgattcca
     5521 tcggtttggg cgaagacggc ccaacccacc agccgattga gcaaaccgcc accctgcgcc
     5581 tgattccgaa tatggacgta tggcggccgt gcgacaccgc cgaatccttg gtcgcttggg
     5641 cagaagccgt caaagccgcc gatcacccgt cctgcctgat tttcagccgt caaaacctga
     5701 aattccaagc gcgcagcgaa caacaactga acgacatcaa acgcggcggc tacgtcatca
     5761 gcgaagccca aggcaacgcc caagccgtca tcattgccac cggttccgaa gtcgagctgg
     5821 ctttggaagc gcaaaaagca ctcgccgcgc aaaacatcgc cgtgcgcgtc gtttccatgc
     5881 cgtccaccaa cgtattcgac cgccaagaca ccgcctatca agccgccgtc ctgcccgaag
     5941 gcctgccgcg cattgccgta gaagccggac acgccgacgg ctggtacaaa tacgtcggcc
     6001 tgaacggcgc agtcgtcggc atcaaccgct tcggcgaatc cgcccctgcc gatctgctgt
     6061 tcaaagaatt cggctttacc gtggacaatg tggtcgatac agtgaaatca gtgctgtaac
     6121 cccacaccta aacaaatgcc gtctgaaacc aattagggct tcagacggca tttgtttgcc
     6181 ctctctcctt gatacttcct cattcctcca atctccatta taatatttgc gaatcactct
     6241 tattcacatt tcaaaaggag aaacgtatga acacccgcac cgaacacgac accatgggca
     6301 atgtcgaagt cccgtccgaa gcctattggg gcgcgcagac ccagcgcagc cgcaacaatt
     6361 tcaaaatcgg cggcgaaacc ctgccgcagc cgctgattta tgctttggcg ctggtgaaaa
     6421 aagccgctgc cgcgaccaat gtttccctcg gcaggattaa gccggaacag gcggatttga
     6481 ttacgcaggc ggcggatgat gtgttgaacg gcaggcttga cggacagttt cctttagtgg
     6541 tttggcagac cggctccggc acgcagtcca atatgaacat gaacgaagtg ctggcgaacc
     6601 gcgccaacga aatcgccggt acgggtttgg cggcgtatca gcccgtccat cccaacgacc
     6661 atgtgaacca cgcgcaatcg accaacgacg cattcccgac cgccatccac gttgccgccg
     6721 cgattgaaat caaccgccac ctcatccccg ccgtaaaagc cctgcgcgac accttggaca
     6781 aaaaagccca agccttcgcc cccatcgtca aaatcggccg cacccacttg caggacgcga
     6841 cgccgctgac tttaggccaa gaattttccg gctacgtttc ccagctcgac cacggcttag
     6901 gccgtctgaa tgacgcgctt aaagacttgt atgaacttgc tttgggcggt acggcggtcg
     6961 gcacgggttt gaacagccat cccgaatacg ccgaaaaagc cgccgccaaa ctcgccgaat
     7021 tgtccggctt gccgtttgtc agcgcgccga acaaatttga agccttgggc ggacgcgatg
     7081 ccgccgttgc cgcttcgggc gcattgaaaa cgctggcggc aagcctgaat aaaatcgcca
     7141 acgacatccg ctggctggca agcggcccgc gctgcggttt gggcgaaatc aaaatccccg
     7201 aaaacgagcc gggttcgtcc atcatgccgg gcaaagtcaa cccgacccaa tgcgaagcga
     7261 tgaccatggt gtgctgccaa gtgttcggca acgacgttac catcggcatg gcgggcgcgt
     7321 cgggcaattt cgagctgaac gtctatatgc ccgttatcgc ctacaacctc ttgcaatcca
     7381 tccgcctgtt gggcgacgcg tgcaacagct tcaacgaaca ctgcgccgtc ggcatcgaac
     7441 ccgtaccgga aaaaatcgac tatttcctgc accattccct gatgctggtt actgcgttaa
     7501 accgtaaaat cggctacgaa aacgccgcca aagtcgccaa aaccgcctac aaaaacaaca
     7561 aatcgttgcg cgaaaccgcc gttgagttgg gcttgctgac gggcgaagaa tttgacgaac
     7621 tggtcgttcc tgccgatatg gttcatccgc gctaatcttt ccctcaaata aaatgccgtc
     7681 tgaaacctcg ttcagacggc attttccgtt gcctgcgaac tagcggcgtt tgaacagcct
     7741 gtcccccacc gccgccgtaa ccgcaccccc gaccacgacc agtgcgccaa cataacccaa
     7801 accgttcata tccggcgcgg caaaagtatc aggcatcaca taatgcccga gcaaagaaaa
     7861 tattacggta aacacgggga gcaaggttgt taccgcgctg actttggaag cctcccaatg
     7921 tttcaacgcc tcgccgaacg agccgtaacc gattaacgta ttcaagcagc aatacgcaaa
     7981 acaaacccac gccaacgtac cgtccaaact tccgatgtgt gccggtttgg caaacggcag
     8041 gaacacggcg gcacttgccg cataaatcaa cagcagaatc tgttgcggtc cgaattgcgc
     8101 cgacagcagc ttttgcgcca cggcataaca cacccatgcc atactgcctg ccgcacacag
     8161 caacacgccc ttcgcatacg cgcccaaacc cgacaactcg ccgaatttat cgttaaaaaa
     8221 cataagcaaa ccggcaagca gcaaaaccaa gccgattttc tgagcggcag tcatccggtc
     8281 tttaaacacc aacacaccga caacaatcat cgtaaacggc gaaatctgcc acaaaacctg
     8341 cgtcgtggtc ggcgaaatat aatgcagccc ttgggcaatc agcacaaagt ttgccgaaat
     8401 gcccgccacg ccgagcagca gcagcctgaa tgagcaccaa gaaaaatccc gccacttcgg
     8461 cagccgcccg cccaatgcca gcaaaacaaa caataccgcc gccgccacgg taaaacgcac
     8521 ccacaccagc gtcggcgcat cgacaaactt caatacctgc cgcacggcaa tcggcagcgt
     8581 tccccacgtc atcgccgcca aaagtgccaa cgcgaagcct aggagcggcc tttggttttc
     8641 catcctgatt ttcctatttt taaacaaccg tattgccgga cgatgccggt ttgccgcatc
     8701 gggcaatgat ggttcaagcg tttgacgttt gattccaacc ctttgatttc aaacaaaccg
     8761 gctgaagctc ggctattgct tcgcgctatt tgaaaacacc gcctgaattt taaaatatag
     8821 tgga
//
LOCUS       DAWEWP010000079         8604 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWEWP010000079 DAWEWP010000000
VERSION     DAWEWP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.079"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(280..3132)
                     /gene="gcvP"
                     /locus_tag="WGN53_001304"
     CDS             complement(280..3132)
                     /gene="gcvP"
                     /locus_tag="WGN53_001304"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ0140294.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDEFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAKADDGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGINVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3163..3522)
                     /locus_tag="WGN53_001305"
     CDS             complement(3163..3522)
                     /locus_tag="WGN53_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140295.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRASLMAMLPL
                     FVVILVLLNIVFPLLAHPLAWLVPAGFMVLVGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCCPCPNHPMSKNSA"
     gene            3470..3700
                     /locus_tag="WGN53_001306"
     CDS             3470..3700
                     /locus_tag="WGN53_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0140296.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKTPYRRNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            complement(3693..4334)
                     /locus_tag="WGN53_001307"
     CDS             complement(3693..4334)
                     /locus_tag="WGN53_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ0140297.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4324..4875)
                     /locus_tag="WGN53_001308"
     CDS             complement(4324..4875)
                     /locus_tag="WGN53_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ0140298.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKRQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4879..5397)
                     /locus_tag="WGN53_001309"
     CDS             complement(4879..5397)
                     /locus_tag="WGN53_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ0140299.1"
                     /translation="MRLFPIAAALSLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGQVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFIPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5397..7037)
                     /gene="pqiB"
                     /locus_tag="WGN53_001310"
     CDS             complement(5397..7037)
                     /gene="pqiB"
                     /locus_tag="WGN53_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ0140300.1"
                     /translation="MTDNSPPPNGHAQAHVRKNNTFLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYADDTVIATQGGGLDDLQVKLADLLDKFGKLPLDKTVSGLN
                     ASLAELKSTLKSINTLSNNPQTQNIPNELNQTLKELRTTLQGVSPQSPIYGDVQNTLQ
                     SLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7030..8490)
                     /locus_tag="WGN53_001311"
     CDS             complement(7030..8490)
                     /locus_tag="WGN53_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ0140301.1"
                     /translation="MGSAVGGQPLEKIFLPDDKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDRGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1926 a         2199 c         2432 g         2047 t
ORIGIN      
        1 gaggcaacgc tgtactggtt tttgttaatc cactatattt gtttgtccgt gtggaaatga
       61 catcaaaggg tgtgaatagg cttttcatct ttgaaatctg tttgcacgat cggatgaata
      121 aaaagtagga tgataaaaaa gctgatgtat ggatactggt ttaactcatt tgttttaaag
      181 ttatagtcga gatacgtgta ttggtgcaac cagtctgccg ttgacgaaaa tgccgtctga
      241 aaatgtttca gacggcattt gtcttcgggg tttcaacggt cagtcttcat aattttccat
      301 cggtgggcag ctgcacacga gattgcggtc gccgtacacg tcgtccacac ggttgacgct
      361 cggccagaat ttgtgttcgc ggacgaatgg caacggaaag acggcttctt cacgggagta
      421 cggatgcgcc cattcgccgg ttacatcgga tgcggtatgc ggagcgttga ccagcggatt
      481 gtcctctttc ggccattcgc cgcgcccgac tttcagcact tcctgtttga tttgtttcag
      541 ggcggcgatg aagcggtcga gttcggcttt gctttcgctc tcggtcggct cgatcatcag
      601 cgtaccggca acggggaagg aaaccgtcgg ggcgtggaag ccgtagtcca tcaggcgttt
      661 ggcgatgtcg gtttcagtga tgccgctttc ggctttgagc ggacgcaaat cgacgataca
      721 ttcatgcgcg acgcggccgt ttttgcctgt atacagaatc ggatagtctt cgctcaggcg
      781 tttggcaacg tagttggcgt tgagcaatgc ccagcgtgtc gcctgttcca tgccttgttt
      841 gcccatcatg gtcaggtaca tccaagtaat cggcagtatg gacgcagaac cgaaggccgc
      901 agcagcaacg gcggtttggt cggcactggc gctgtgggtg tcggtcaaag cgtgtcccgg
      961 tgcgaacgga gccagatggg ctttcaagcc aatcggaccc atgcccggac cgccgccgcc
     1021 gtgagggata cagaaggttt tgtgcaggtt catgtgcaac acgtccgcgc cgacttcggc
     1081 aggctgcatg atgccgattt gggcgttgag gttggcgccg tccatgtaaa cctgtccgcc
     1141 gttttcatgg acgatgcggc agatgtcgcg gatgccttct tcgtacacgc cgtgggtgga
     1201 cggataggta atcatgatgg cggacaaagc gtcgcggtgt tgctcggctt tggctttcaa
     1261 atcgtcgatg ttgacgttgc cgtgttcgtc ggtatcgacg acgacgactt tcaaaccgag
     1321 catggcggcg gtggcggggt tggtgccgtg ggcggatttg ggaatcagac agatgttgcg
     1381 ctgcgcttcg ccttgcgctt cctgatagcg gcggatggca agcataccgc tgtattcgcc
     1441 ctgcgcgccg gagttgggct ggaaggaaat tgcatcaaaa ccggtgatgg atttcaggct
     1501 gttttccata tcggcgagca attcgcggta gccggcggtt tgcgcttcag gagcgtaagg
     1561 atggatgtcg gagaactccg cccaagtaat cggcaacatt tccgcagtcg cgttgagttt
     1621 catggtgcag ctgcccaatg aaatcatgct gcggttcatc gccaagtcgc ggtcttcgag
     1681 ttttttcagg tagcgcagca tttcgtgttc ggtgtggtaa cggttgaaca cgggatgttg
     1741 cagaatgtcg tcttgacgca gcagttcggc gttcagacgg cctttgacat catcggcaaa
     1801 tgcgaacgta tccttgccgg taaacgcgcg gtacaaatca accaaatctt cgcatgccga
     1861 tgtttcgtgg aatgcagccg caacttgagt atcgttaacg cggcgcaggt tatagcccga
     1921 ttccaaagca gcggcaaaca cttggtctgc tttctctttg ttgccgaaat cgacggtaac
     1981 ggtatcgaag aagactttgt gaaccacatt aatgccgtct gaaaccagcg catcggcaaa
     2041 agcagaagcc agcgcgtgaa tgcggttggc gatgcgtttc acgccttcag ggccgtggta
     2101 aacggcatac atacccgcca aattcgccag caatgcttgt gcggtacaaa tattggacgt
     2161 ggctttttcg cggcggatgt gttgctcgcg ggtggacaac gccatgcgca aggcaggttt
     2221 gcctgatgcg tctttggata cgccgatgat gcggccaggg gcggaacgtt tgaacgcgtc
     2281 tttaaacgcg aaataagcgg cgtgcggccc gccgaagccc ataggcacgc cgaaacgttg
     2341 ggtgttgccc aaagcaatat ccgctcccaa ttcggcaggt gatttcagta aaaccaagct
     2401 catgatgtcg gcggcaacag caacgatcgt gccttttgct ttcagacgac cgataacatc
     2461 ctgcaagtct tgcacatcgc cgtctttgcc gacgtattgg aacagcgcgc cgaagtattc
     2521 gccgtcatcc gctttggcaa aatcgctgac caccagctcg aagccgaaat atttggcgcg
     2581 ggttttcatc acgtccaaag tttgcggata cacgcgcgca tccacaaaga aacgctcgga
     2641 tttcaccttg cccacgcggt gcgccatcgc catcgcttca gccgcagcag ttgcctcgtc
     2701 cagcaaagac gcgcccgcca cagggaaacc ggtcaaatcg atacacactt gttggaagtt
     2761 taacaacgct tccaaacgac cctgggcaat ttccgcctga tacggcgtgt aggcggtgta
     2821 ccaacccgga ttttccaaca cgttgcgcaa aatcacgttc ggcacgcggg tcgggtaata
     2881 gcccaaaccg atataggact tgttgatcac gtttttcgac gcaatgcctt tcaatttcgc
     2941 caacgcgtcc gcttcggtca gggcatcggg caaatcgagt tcggacggca tacggatgct
     3001 ttgcggcacg gtattgccga caaattcgtc catgcttttc tcgccgacag cggcaagcaa
     3061 cgccgcttcg tcgccgaaac tcaaatggcg cgcggcaaat tcgttcgggt tgaacaattc
     3121 agatagtttc atttctactc cttcgggttt gtagggctgc catcaggcag aatttttaga
     3181 catcggatga ttcgggcaag ggcagcacaa aagacgggat aatggcggac aatgcgccag
     3241 aatgcagcag gtcaggacaa gcagcgccac aagcggcagc gataagggaa agccgccgac
     3301 caaaaccatg aaacctgcag gcaccagcca agccaaggga tgcgccagaa gcggaaaaac
     3361 aatattgagc agaaccaaaa tcacaacaaa cagcggcagc atcgccatca ggctggcacg
     3421 cctgtatgtt gcgtacgggt cggatgcttc ttcaggttcg caatattcca tgatttcagc
     3481 ctgtcctggc tttatccaca gcaggctcgg acggtcggtc atgccgtccg aaccggcagg
     3541 ccgtcttttt caaaaaacgc cataccgccg caacttcatg cggctgtatg gcgtaggttt
     3601 tgcacttcag acggcatatg cgtcccgaag cagtttggtg ttccagtcgg ttcatgatta
     3661 tataaacaac ttgggtgcgg ataaaccttt gtttatctaa acccctgttt tctcaaaaat
     3721 tgattcaaat cttcggcaaa tttccgatag cctttgccgt tggcgtggat ttggtcggat
     3781 ttcagattat tatcgcccaa aatttccgcc cacgcgccgc cgaacagcgg aatgccgtat
     3841 tcctcggaca aatcctcata cagcggatga tcgctcaaat gcccgaacaa cgcgcccagt
     3901 gtgatgtgcg gcacgccgac gaggacggcg gggatgtttt ccttctgcac ggtttcgatg
     3961 attttcgcga tattggcgcg ggtctgctcc ttgggaactt tgcgcagaaa gtcgttgccg
     4021 cctatgccga caatcacaag cttgggtttg cgtgccaaca gcgcgggcag gcgcgacagg
     4081 gcttgggcgg atgtatcgcc cgatacgccg ccgttgacaa tattccaacc cgtcagtttt
     4141 tgcagttgcg cggggtagga ttcgccaggg tttgcgccgt agccgaaggt aagcgaatcg
     4201 cccaaggcaa gtacggtgct tccttcggga attttggcgt gggttcgggc ggattttctg
     4261 ccgcaggcgg taaggagcaa cgcgcctgcg ccgaggagga aggttcttct gttcattggt
     4321 ttttcagatg gcatcgagcc atttcgggtg gtatttcagg gtttcacggt aaaccggaca
     4381 gctttcggcg tgcgcgcctt tgaggtagcc ggtactcatc agaaattcgc cgacgatttc
     4441 gccgccgacg aatttgaaat gttttttaaa gagtttaacc cattcgtctt tgcttcgcgg
     4501 atggtgcgtg tcgagccagt tcttgaacga gccgtattct tgttgcaacg cttggatttg
     4561 ccgtgcattg aaaatggcgg catcgatttt caggcggttg cggacaatgc ccgcgtcggc
     4621 aagcaggcgt tcgcggtcgg tgtcgtcgaa ggcggcaacc gtatcgatgt cgaaaccttc
     4681 aaatgccgtc tgaaacgcct gccgcttctt cagcatcagc gtccagttta atcctgcctg
     4741 attgatttcc aacaccagcc gctcaaacaa ttcattgtcg tcctcaatcg gaaaaccgta
     4801 ttgcgtgtcg tggtaatgtt tgttcgggtt atcggtgttt tcgggaagtg aggcggcaaa
     4861 ttcgcaatag ttcacgtttt actcgaccat ctgttgcgcc gcctgtttca gtccctgttc
     4921 gagtgcggcg gtcatcgcgg cgtagccgtc accctgctgt tcggtttcga tatggaaggg
     4981 tctgttcgta ccgtcgggta ggacggcgta gccgctgatg agggttttgc ccgtgtagct
     5041 gccttggaat gcgtcaatat agaccgtcca tttttcggta ctgccgctgc gtgaggcagg
     5101 aataaagatg cgtgtgctgt ccaaacggtt gaatgcattg ctcaacgccg cttcgagcat
     5161 atcgtccaag gtgtccgccc aaacgtggtt ttgtgcggtg ttgaggcggt aggggtcggt
     5221 ttgatagacc tgtccgccgc gtttgagcgg ttcggcaaga cggacttcga cggcagtttc
     5281 gccgccttgc gttgcaggac ggatgtagcg gctgtcgggc aacacgaaat attgtgtgct
     5341 ttgcacagta ccgcaggcgg caagcgacag ggcggcggca atcgggaaaa ggcgcattat
     5401 cggcttcctt tcgggatagg gtctttgctg ctgctgttga aaatcagcgc gttgggtttt
     5461 tctttcaaag tgttaatgac gggttgaacg tcttttaagg ttttgtccaa actttgcagc
     5521 gtattttgta cgtcgccgta gataggcgat tgcggcgata cgccttgcag ggttgtgcgc
     5581 aactctttca gggtttggtt caactcgttc ggaatgtttt gcgtttgcgg attattgctt
     5641 agcgtgttaa tggatttcag cgtggatttg agttcggcga gcgaagcgtt taaacctgaa
     5701 acggttttgt ctaaaggcag tttgccgaat ttgtccagca aatccgccaa tttgacctgt
     5761 aagtcgtcca aaccgccgcc ttgtgtagcg atgacggtat cgtctgcata aacggtatgc
     5821 ggtcgcagct tgggcgaaga cgaaggctga tcgttcaact caatcatttt gctgccggtc
     5881 agcaggttgt tgctggagat ggtggcggtc aggcctttgt ttaaagccgt ctgaaattgt
     5941 tgtttccaat gctctttgct ttgttcgtcg gcattgattt ccaaacggga aggctcgatg
     6001 cggatgcgta cgggaatcca gccgttttca aataaatgca ggctgtcgtt gcggtcaaaa
     6061 taagggacat cggaaaccac gccgacattc agccctttgt actcgacggg cgaaccgacg
     6121 gtcaggccgc gcacggattg tttgaaaaac gcggtgtagt acagcgagcg gtcgtcaggc
     6181 aggttggcga cttcgctgcg gctgtcgtaa agcgtgaagc tgtcttcgct tttgacgttt
     6241 ttactgtttt tggttttcgg cgaatcaaat gaaatcgcgc ccgacagcag ggcaggcaga
     6301 ggggcggaat tgagtttgat gccgctgcct gtggtttcga tattgatgcc gctttccagc
     6361 caaaaacggc tggcggaatg aatcagtttg tcgttggggc tttggatgaa gatggtgtaa
     6421 tgcacgcttt ggtcggacgg gtcgaaatgc gcgctttcga cttgcccgac cataaagttt
     6481 tcatacaaaa cagggctgtt gacgttgagg atgcggtcgt ttttaccaat caaattcaag
     6541 cgcagcccgc tttgcccgat ggcggtaacg ggcggaatgt cctgcacttg gaacacgtct
     6601 tttgcctcgt cgcttttgcc gggtgtaaaa gcgatgtacg aacccgaaag cagcgtaccc
     6661 aaaccggtta cgccgctttg gtcgatacgc ggcttgacca cccaaaactg ggtatcgctg
     6721 cggatgaggc cggatacgtc cgcattgagt tgggcggtta cttccacgcc tttttggtcg
     6781 tcgcgcagtt tgattcgggt aacgcgtccg acatcgatgc tcaatacttt gatgaccgta
     6841 ttgttgacct caatgccttc cgcgctgtcc atcaagagcg taaccacagg ccccctgttg
     6901 cggatttcct taatccacag ccagccgccg gcaatcagcg cgatcagcgg aaccagccag
     6961 acggcagaga ggaaggtgtt gtttttgcgg acgtgtgctt gggcgtgtcc gtttggagga
     7021 gggctgttgt cagtcatgtt tttccgtttc gttgaaagca atgccgtctg aagcgcgttt
     7081 gtcccaaagc aggcgcgggt cgaaataata ggcggacagc atcgtcagaa tcacgaccag
     7141 gcagaaatag actgccgcac tgcccggaat gacgcgcgcg gcataagtgt ggaacgaaca
     7201 cattaaaata ataatcacaa aaatatcaat catcgaccag cggccgaccg cttcggtgat
     7261 gcggtagagg tgtgacaatt tctttgcgcc tgtcggcaaa gcgaagcggg cggacgcaat
     7321 taaaaccgac attgccgcaa tcttcagcac cggcaccaaa atactcgcgc tgaaaataac
     7381 cgccgcaatc agcctgtcgc cctcgtccca catataagcg atgccgttaa ggatggtatt
     7441 gacctccgtg gcggcaggat tggacgaaat cataatcggc aggatattgg cagggaaata
     7501 caaaataacc gccgccgtca gaaacgccga cgaaatactc agacttttcg gccgtcggcg
     7561 gtacagttcc gcgccgcaca caccgcaggg ggattcggca ctgtcgcgga aatacaggca
     7621 gcggctgcaa caggttttgc cttccgatgc cgtctgaacc gcattatccc ccgtcagccg
     7681 cccgatttga aaatacaccc aatgctgggg aacggatacc gaagtccgaa tcagcataac
     7741 cgacagcgcg aacatcagat aaaacgccga cccgaagcga acctctgcca cagacgagag
     7801 cttgatatac gccaccagcg tggaaacaaa aaacacatcc accatcatcg cctgcctcaa
     7861 gcgcaccatc acacgcgttg ccaaacgcag cgcaggatac acccgtttcc gtatcagcgc
     7921 ggcatagaca tacaggcaca gcagcagaaa cagaaccggc gcgccgaagg tcagcacaaa
     7981 catcacttcg gccaaaaaac cataatcctg aaacaccatc aggcgcatca tctcgggcag
     8041 cgaaaggacg gatgccgcac ccggtatccc gacctcgata tacgtcatac cgtaagcaaa
     8101 cgccatcaaa atcagcgaag ccgccgcata agcgggcggg gcggaaaaag gatgcctgcc
     8161 caccctgaag agcttatagc cgcagcgcgg gcagaacgcc gcctcccccc tgtccaaccg
     8221 aggcacatcc gcgcggcagc cgcattccgg gcaatctacc gtatgcggcg gtagggcttc
     8281 gtcgcggcag ttgccgcgaa ggctgtgggg gatattttcg gcaaacggct tcatcgggaa
     8341 agtggcaaat ataagtggcg gcattataaa ggaaaaaagg cgatacaccc aatcaggatg
     8401 caaaaccggc aggttcggca gtatgaacaa acccttgtcg tccggcaaaa atattttttc
     8461 gagcggctgt ccgccgactg ccgaacccat ttgataaaat cccgattctg taaagaatag
     8521 taaaatacct gcttaaaaac atcacgcaaa tgatattttt tccaaaaata tgtgataata
     8581 aatttcgttt atgattcttt atta
//
LOCUS       DAWEWP010000080         8500 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWEWP010000080 DAWEWP010000000
VERSION     DAWEWP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.080"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            83..1267
                     /gene="coaBC"
                     /locus_tag="WGN53_001312"
     CDS             83..1267
                     /gene="coaBC"
                     /locus_tag="WGN53_001312"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ0140302.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1334..3490)
                     /locus_tag="WGN53_001313"
     CDS             complement(1334..3490)
                     /locus_tag="WGN53_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0140303.1"
                     /translation="MPVPQPTAPYDHLTAEARTLLFRTASYLKPEEQVELEKAVAYAF
                     HAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3583..3789)
                     /locus_tag="WGN53_001314"
     CDS             complement(3583..3789)
                     /locus_tag="WGN53_001314"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ0140304.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WGN53_001315"
     CDS             complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WGN53_001315"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ0140305.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4593..5192
                     /locus_tag="WGN53_001316"
     CDS             4593..5192
                     /locus_tag="WGN53_001316"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ0140306.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5238..6026)
                     /locus_tag="WGN53_001317"
     CDS             complement(5238..6026)
                     /locus_tag="WGN53_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ0140307.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVHEVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            6351..7706
                     /gene="yegQ"
                     /locus_tag="WGN53_001318"
     CDS             6351..7706
                     /gene="yegQ"
                     /locus_tag="WGN53_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ0140308.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLNVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDARLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDKNGWATIEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMRGKEKALIARVLN
                     P"
     gene            7840..8316
                     /locus_tag="WGN53_001319"
     CDS             7840..8316
                     /locus_tag="WGN53_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ0140309.1"
                     /translation="MPAVCQSGSAPKNAHTASAGTPAGESCKVIRDDTAGSGKNIIYR
                     CSNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQTGSFHVRVVMRANVVR"
BASE COUNT         2165 a         2327 c         1998 g         2010 t
ORIGIN      
        1 ttgtatccaa acggtatcta atgtatccac actttgttat agaatggctg ctgttttttc
       61 ttcgtaatta gaaattgtca aaatgggcaa acatattctt ttaggtgtaa cgggcagtat
      121 tgcggcgtat aagtcttgcg agttggttcg tctgctgaaa aaacaggggc attcggttac
      181 ggtggttatg agccgctcgg caactgaatt tgtttctccg ctgacttttc aggctttaag
      241 cggcaatcct gtcctgaccg acacgcacgg cggcaacggt tcaaacggta tggaacatat
      301 caacctgacc cggaatgcgg atgtttttct gattgcgccg gcaagtatga ataccgtggc
      361 aaaaatctgt aacggcgtgg cagataacct actgaccagt ctggcagccg cacggaaatg
      421 tccgcttgcc atcgctcccg cgatgaatgt ggaaatgtgg ctcaaccctg ccaaccaacg
      481 gaatatcgca caactggttt cagacggcat tactgtctat atgccgggct tgggcgaaca
      541 ggcttgcgga gaaaatggca tgggaaggat gccggaacct gccgaattgc tggatttgct
      601 tccggacttg tggacaccga aaattttaag gggcaagaaa atcttgatta ccgcaggtgc
      661 gacatttgaa gccattgacc ctgtcagggg catcacaaat acctccagcg gaaaaatggg
      721 ggtagctttg gcgcgggcgt gccgtgccgc cggtgcggaa gtcagcctga ttcacggaca
      781 gcttcaaacc gcgctgcctt tcggcatatc cgatacggtt caagccgtca gcgctgaaga
      841 tatgcatcgc gcagtgcatc gtttgattga aaaacaagat gcttttattt ctgttgccgc
      901 cgtctcagac tataaggtta aaaacaggag tacacaaaaa ttcaaaaaag ataaaaatgc
      961 caaaccgtta tccatcgaat tggatgagaa ccccgatatt ttggcttcta ttgcctcatt
     1021 gccgaacccg ccgttctgca tcggttttgc cgctgaaacg gagaatgtaa tggcatatgc
     1081 acgagaaaaa cgtattaaga aaaaaatacc ggtgatagtt gccaatgatg tttcaatcgc
     1141 gatgggcaaa ccgaccaacc ggattaccat tatcggggac gacggggaac tatcttttcc
     1201 cgaaacaagt aaagatgaag cggcgatgca gattgttgaa aggcttgccg tatatttgaa
     1261 caaataagca attgaacgga taaaccataa aacgggttgc ctgttaatca aaaggcaacc
     1321 cgttttacct gcttcaactt ctgatgactt tgcggatata tggaatacta tgcagatttt
     1381 gaataatctg attcaactga tccaaattct tgactttcaa taagaatttg aattcgacaa
     1441 aaccttccgt tcccgactgg gatttagacg gtgtttcgac cgactcgatg tccgcaccgg
     1501 aatcggaaat cgcttgcgcc attaatgcca acaggccgtg gctgtcttcc gattggactt
     1561 gaagcccgac acggtagttc tgcccgttca tattttccca gtctgcatcc agctgctgtt
     1621 cgggatcgga cttcaacaac gtcgggcagc tatccctatg gataatcatg ccttttccct
     1681 taaccaacag caaacggatg gaatcgccgg gaacagggtg gcagcactct gcaaaatgaa
     1741 tatgcccgct ttcctgacca tcgactttaa tggaactgag cctgacctcg ctgccgaaat
     1801 gctcccctgc caactcagca atgtgcatgg cgacataaac aggcagggta tgccccatcc
     1861 ctacgttgta cagcacttct tcaaacgatg tctgcttgtc gttgagatcg gcaagatatt
     1921 tttccttgat gccgtctgaa agcaggacat ctttgggcag caaactggac agggcttttt
     1981 gtaagaggct ctctcccaaa acgaccgcat cgtgccggtt aaggttttta atatattggc
     2041 gtatggcact gcgcgccctg ccggacacgg cgaaattcaa ccacgcggga ttgggtttgg
     2101 cgtgttcgga tgtgataatt tcaacagaat caccggtttt gagcttcgta cgcaacggca
     2161 tcatgatatt gttgatacgt gcggcaacgg ttttgtgccc gatatcggta tgcaccgcat
     2221 aagcaaaatc gacaggcgtt gcccctttgg gcaaagttag gatttttcct tttggcgtaa
     2281 ggatgtagat ttcgttcgga aacaaatcga ctttgacgtg ttcgagaaac tcaatggcat
     2341 tggcactgct tgcctgcaaa tctaagatat ttttcagcca ccggtttgtg tgaagcaccg
     2401 cctgatcgac cgtcttagaa tatgatttat agctccaatg tccggcgatt ccaccttcgg
     2461 caacagcatc catttccttg gtacgtatct gaacttcaat cggcaagccg taagggccga
     2521 ccaaagtcgt atgcagactt tgatacccgt tgcttttcgg aatggcgata tagtctttga
     2581 accgcccggg cttgggctga tagagggtgt gcaatgcgcc gagtgcggca taacaggctg
     2641 gaatgctgtt gacaatgacg cggaaaccgt aaatatccat aacctcggca aagcgcagct
     2701 ttttcgccat cattttctga tggatgccgt acaggttttt ttccctgcct ttgattttgg
     2761 cctctatatt cgcgcctacc agccgctggc cgaatgcgcg caagactttg ccgacaacgt
     2821 cctgccggtt cttccggctc ttgtccatcg ctttttttaa agtctcgtag cggttgggat
     2881 gcaggttttg gaacgataaa tcctgaagct cttgatatgc gttattcaaa cctatacggt
     2941 tggcaatctg tgcatagatt tcaagggttt cccttgcaat ccggcggcgt ttgtccgggc
     3001 gcatcgaacc gagtgtccgc atattatgca gacggtcggc aagtttaacg acaatcacgc
     3061 gcacatcttt ggtcattgcc aaaatcagtt tgcggaaact ctccgcctga tgttccgcat
     3121 gatcttcaaa tttgagtttt tcgagtttgg acagaccgtc caccatctcg gcaatcgtat
     3181 tgccgaacac cgccgccatt tccccttttg tcacgcccgt atcttccaat acgtcgtgca
     3241 tcacgcctgc acacagaccc tgtatgtcca tatgccaaag ggcgagctgc gtcgcaacgg
     3301 caattggatg cgtaatgtat ggttcgccac ttttccgggt ttgtccgtca tgagcgtgaa
     3361 atgcataggc aacggccttc tcgagctcaa cctgttcttc aggcttgaga taggaagcgg
     3421 tacggaaaag caaggtgcgt gcttcggcag ttaaatggtc gtaaggtgcg gtgggttgag
     3481 ggacaggcat ttcagacggc tttcggaaca tattttattt ttcgtttcaa accgtcggac
     3541 tgcacggcgg cagaatgtgc cgacgtgcgt ttccggcaga atttatttat tgcgcgtcaa
     3601 cagttccgta ccgatatgcc cggcggcgat ttcccttaag gcggtaacgg tcggtttgtt
     3661 attgcggaca tcgtccacaa gcggcgtatt gccattttca agctggcggg cccgacgggc
     3721 cgctaccaat gtcaggtcaa aatggttgga aatttttccg gtacagtctt cggtggtaat
     3781 acgtgccata ttatttgctt tctttcaaaa atatttaaaa taggaaactg ggtattttcg
     3841 cggtttttta ggaattttcc aacaaatctg caataaatcc cagttgccgc gaccttttca
     3901 gacggcaggc attcacaata tggcgcaaat cctcctccgc ccgcgccaag tcatcattga
     3961 ccacgacaaa atcgaacaat acggactgtt cgatttcatg ccttgccttc gacagcctcc
     4021 tttggataac ttcccgactg tccgtcccgc gtccgttcag acgtgcagca agcacatcga
     4081 aagacggcgg caggataaag atgccgacgg cttccggcag cgcgttgcga acctgcgccg
     4141 cgccctgaac gtcgatttcc aaaattacgt catagcctgc cgccgccaac gcattcacgc
     4201 cctccgcgct tgtgccgtaa tagttgccga acacgtctgc gtattccaaa aaagcttcct
     4261 gcgcgataag cgactcaaac tcttctttag aaacaaaatg ataatgtacg ccgtttactt
     4321 cgccttcacg cggcggacgc gtcgtgtgcg atacggaaac gcgcaaaccg ttatggtttg
     4381 ccaacagccg cgacaccagc gtggttttgc ccgtgccgga agcggccgaa atgataaaga
     4441 tgttgccttt tcgataagcg gacatatttt ttacctgtat attttccagc cgattgtatc
     4501 acaacggaca ctcggtttcc tatttgccga tgcccatatt ttgccgctat tgttttgatt
     4561 gatttggcaa gcggcaggct gacggctaca atatggcgtt aaaaacatca aacttggaac
     4621 acgcaatgct ggttcatccc gaagctatga gtgtcggcgc gcttgccgac aaaatccgca
     4681 aaatcgaaaa ctggccgcaa aaaggcatct tattccacga catcacgccc gtcctgcaaa
     4741 gcgcggaata cttccgactt ttggttgatt tattggttta ccgctatatg gatcagaaaa
     4801 tcgacatcgt tgccggtttg gacgcgcgcg gcttcattat cggcgcggca ctcgcctacc
     4861 atctcaacgt cggttttgtc cccatccgca aaaaaggcaa gctgcctttt gaaaccgtat
     4921 cgcaaagcta cgcgctcgaa tacggggaag ctgcggtgga aatccacacc gatgccgtca
     4981 aacccggttc gcgcgtgctg ctggtcgatg atttggttgc cacgggcggc acaatgcttg
     5041 ccgggctgga actgatccgc aaactcggcg gagaaattgt cgaagctgcc gctattttgg
     5101 aatttaccga ccttcaaggc ggcaagaata tccgtgcaag cggcgcgccc ttatttaccc
     5161 tgcttcaaaa cgaaggctgt atgaagggct gaaaaccgac cctgccgtct gaaaccggca
     5221 gggttgttat tatgcgttca aatcacgccc aaatcttgca agcccctcaa cacgccgtct
     5281 tcatcaacgc tggggcaaac atatttcgcc gcttctttcg ccgcctgttc cccgttgccc
     5341 attgccacgc cgaacccgac ttcagacagc atttccacat cgttcaaacc atcgccgaac
     5401 gccatcacgt ccgccatttc caaacccaat gcttcaacca cgcttctgat gccgtctgtt
     5461 ttcgacgcac ccgcaggcag cagatcgacc gcttcctcat gccagcgcac cgttttcaag
     5521 ccttcccgtt ccacaatatc cgaccaaagc ggcatttcgt tttcctccgc aaacaccagc
     5581 atctgataca ccggtttgct tgaaaaataa tccttatcgg caaaaaaatc gctggcgata
     5641 tgcttcaagg cgcggcacac gcattccgac agtgcggaca ccgcaatccc ctctccgccg
     5701 acaaacgcat aatccatgcc caagccatcc aaatgcgcgc aaaccctgcc catcaaaccg
     5761 gcatccatcg gtacttcgtg cacagttttt ccgtgcagca gcgcaaactg tccgtttatc
     5821 gttaccacgg catccatacc cgtttccgcc atcatgtccc tgaccttttc gggaatcgtc
     5881 gccaaagacc gccccgttgc caacgccgtc aatatacctt tgccgcgcaa agccgccacc
     5941 gccgttttca cggaaggacg caaggtatcc gtatatttgc ggtacagcgt atcgtcaata
     6001 tcgaaaaaca cgattttggg attcatcaca ttctctctcg cattcaaatt gccgcattgt
     6061 atcccaagca ggcaaatact tgataaatcc ttataaattt cccggcaaaa ttgaccgcga
     6121 acacgaaaag gcggataatc cgcccatcct caaacccttt tcagacggca tttgcagcaa
     6181 tgccgtctga aacattttta caaagcacac aaatcatgtt tcaacacaca ggacgacaca
     6241 taaagcgtcg ccctatatgt tgccctgatt cggaaagggt tacacccctc ccaaataaag
     6301 tctgattcta ccgccctaaa gggcggggtt tcaaccgaaa aggaaacacg atgaaagcac
     6361 ccgaactctt attgcccgcc ggcggattgg aaagaatgcg cgccgcctac gattacggcg
     6421 cagatgccgt ttacgccggc agcccgcgtt actccctgcg cgcccgcaac aacgaatttg
     6481 ccaaactcaa cgtccttgag caaggcatta aagaagcgca cgagcgcaac aaaaaattct
     6541 ttttgaccgt caacaccctg ccgcacaact ccaaactcaa aacctttgtt tccgatatgg
     6601 aacccttgat tgccatgaaa cccgacgcgc tgattatggc ggatccgggt ttgatcatga
     6661 ccgtgcgcga aaaatggccg gaaatgccga tccacctgtc cgtacaagcc aacaccacca
     6721 actactgggg cgtgaaattc tggcagaata tcggcgtcga acgcatcatc ctgtcgcgcg
     6781 aattgagtat ggaagaaatc gccgaaatcc gccaacaatg ccccgacatc gaactcgaag
     6841 tcttcattca cggcgcattg tgcatcgcct actccggccg ttgcctactc tccggctact
     6901 tcaaccaccg cgaccccaac caaggcacct gcaccaactc ctgccgttgg gactacaaag
     6961 tccacaacgc tacggaaagc gatgcgggcg atgcccggct tctgcaaggt ttcaactttg
     7021 aaaaagccca agaagaagcc aaccaaaact ttgaaggcat caacggtcaa aaacgccatc
     7081 cctacgccga caaggttttc ctgattgaag aatccaaccg tccgggcgag atgatgccga
     7141 ttatggaaga cgaacacggc acctacatca tgaactccaa agaccttcgt ggcattgaag
     7201 tggttgagaa actcgccaaa atcggcgtgg acagcctcaa agtcgaaggc cgcaccaagt
     7261 cgctctatta tgtcgcacgc gtcgcccaat cctaccgcaa agcgattgac gatgccgtcg
     7321 caggccgtcc gtttgattac agcctgttga gcgaactcga aggccttgcc aaccgcggct
     7381 acaccagcgg cttcctcgaa cgccaccaca ctcaggatta tcaaaactac ctgaccggcc
     7441 attccaccgc caaacaaagc caatacgtcg gacacgttac cgaaatcgac aaaaacggct
     7501 gggcgaccat cgaagttaaa aaccgctttg ccgtcggcga ttcgctcgaa atcatccacc
     7561 cgaacggcaa ccaaaccatc aaattggaac aaatgacccg caaaggccaa cctgtcgatg
     7621 tcgcacccgg caacggcatc caggtcaaaa tccccaatat gcggggcaaa gaaaaagccc
     7681 tcatcgcacg cgtgttgaac ccctgagcca caatgccgtc cgaaccccgt tttcagacgg
     7741 cattttgttg aatcttacgg tacaatggca atgatttccc acagcaaaca aggacatccg
     7801 acatgaaaaa aaatcctcat actctccgcc gccgccctga tgccggcagt ctgccaatcc
     7861 ggcagcgcac ctaaaaacgc gcacacggca tccgccggca caccggccgg agaatcatgc
     7921 aaagtcatcc gggacgacac cgcaggaagc ggcaaaaaca tcatttaccg ttgcagcaac
     7981 ggtcaggcgg caataaatgc agccattgca gacggcgtat tggaatcagg gattcccgtc
     8041 agcttcggcg gcgggggcgt accggttgca aacggcagga atctggttac acggcaagca
     8101 gcaaacggcg ttggcaaatc cgactccgag gcctgcgaac acgccctaat caatgccgcc
     8161 cgcaaattcc agcagaccgc aggcaaactc ggcgggcgca gtgttaccgg cttccacagc
     8221 tacttcgaca agcagccgct gcaaggcggg caatacgact gtcagacagg ttcgttccac
     8281 gtccgcgtgg tcatgcgcgc caatgtcgtc cgttaacata tcggcaatca aaaaattagg
     8341 ctgtgtttta gtaatctgtt gatttcaatt atttgcaaag gaaaagacaa ttattttttc
     8401 ggttaggaag agacctatcc tattgaatac atttaagtta aatacgtcta tcaacactat
     8461 attatagtgg attaacaaaa atcaggacaa ggcgacgaag
//
LOCUS       DAWEWP010000081         8354 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWEWP010000081 DAWEWP010000000
VERSION     DAWEWP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.081"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(254..1186)
                     /gene="cysK"
                     /locus_tag="WGN53_001320"
     CDS             complement(254..1186)
                     /gene="cysK"
                     /locus_tag="WGN53_001320"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ0140310.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1380..1985)
                     /locus_tag="WGN53_001321"
     CDS             complement(1380..1985)
                     /locus_tag="WGN53_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140311.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1982..2833)
                     /gene="dapF"
                     /locus_tag="WGN53_001322"
     CDS             complement(1982..2833)
                     /gene="dapF"
                     /locus_tag="WGN53_001322"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ0140312.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPELGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            2970..3266
                     /locus_tag="WGN53_001323"
     CDS             2970..3266
                     /locus_tag="WGN53_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140313.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANRILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3280..3948
                     /locus_tag="WGN53_001324"
     CDS             3280..3948
                     /locus_tag="WGN53_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0140314.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(4020..6140)
                     /gene="pnp"
                     /locus_tag="WGN53_001325"
     CDS             complement(4020..6140)
                     /gene="pnp"
                     /locus_tag="WGN53_001325"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ0140315.1"
                     /translation="MFNKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTSV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6377..7240)
                     /locus_tag="WGN53_001326"
     CDS             complement(6377..7240)
                     /locus_tag="WGN53_001326"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ0140316.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPANVIQKTVEK
                     YQEALTLLTQD"
     gene            7439..8302
                     /gene="rfbD"
                     /locus_tag="WGN53_001327"
     CDS             7439..8302
                     /gene="rfbD"
                     /locus_tag="WGN53_001327"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ0140317.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         2000 a         2404 c         1929 g         2021 t
ORIGIN      
        1 gtggattaac aaaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtctg
       61 cggcttcgtc accttgtcct gatttttgtt aatccactat aaaaaatatt tccctcaagc
      121 gatgtggcgg caccaacacc tgattaaaac acaaccggcg ttttcggcat atacgcaaaa
      181 acgcattttc cggcacggcg ttacaccgat accgaaaaat gcgtctgaat gtttcgacaa
      241 tccgattaaa gcattatgcc aaatctgcaa aaagtggcgt agagagatag cgttcgccat
      301 aagaaggcag cagcacgact atcagcttgc cttcgttttc aggctgtttg gcaagctgca
      361 acgcactcca aaccgccgca ccggaagaaa tacccaccaa aatgccttct ttttccgcta
      421 ttgcgcgggc ggtttcaaaa gccgcttcgt tcgacacttt ggtaatgctg tcgtaaattt
      481 tggtattcaa aacggtcgga ataaagcctg cgccgatgcc ttgaatcggg tgcgggcctt
      541 tttcgccgcc gctcaatacg ggtgaagctt caggctcgac ggcaaccact ttaacttcgg
      601 gtttgtattt tttcaacact tcgcccacgc cggtaatcgt accgcccgtg ccgacgccgg
      661 caacgaagac atcgactttg ccgtccgtat cccgccaaat ttcctcggcg gttgttttgc
      721 ggtggacttc ggggtttgcc tcattgtcga actggcgcgg cataaaataa gtgtcgggat
      781 gcgcgtccac caaggatttc gctttggcaa tcgcgcccgc cataccttcg gcggcagggg
      841 tcagaatcag ctccgcacca aacgcgcgca acagcatttt gcgctcctta ctcatgcttt
      901 ccggcatggt aatcgccagc ttgtagccgc gtgcggcaca taccattgcc aaaccgacac
      961 ccgtattgcc gctggttgct tcgacaatga cggtgttttt gttgattttg cccgcttttt
     1021 cggcaccctc gatcattgct tcggcaatgc ggtctttgac gctgctgcct ggattgaaaa
     1081 attccagttt cacggcaacc tccgccttca aaccttcggt cagacggttc agtttgacca
     1141 aaggcgtgtt gccgattagt tcggtgatgc tgtttgcaat tttcatcttc tgctcctgaa
     1201 taataaggtt tcgacagtaa ggcgcaatcc ccataggcat cctgtccgat tgcaggggat
     1261 ttgcgccgaa acataaacaa atttcggata tggctttatc ctaaacctat gccgcctaaa
     1321 aaaccaatat ccaaaatttc tttctttata actttttgtt ccgatacggc cgcaccgtat
     1381 tatcttccaa tttctgaaag cagcagcacc agtaccgaga cctgcatcaa cgctgccgtc
     1441 aacccgccga gtcgcagctg cgagccgccg aacgttgacg gcggctgccg gtctataccg
     1501 cagcggtgga cataggcaac cgccatcagc acaaactgca acacgaacca ataaaccggc
     1561 ttcagcgcat aaagtttgag cagtgccgca aacacaaaca cttgaaccgt tcccgaaaag
     1621 caataatgca ccaacgcaca tattccgaca aaagcaagcg tcatcgatac attactgacg
     1681 gcaaacagcc cgagcagcgg atgttcgggg tctgcctgcc atccgtttcc atccgttttc
     1741 cggttccaat gcccgatgaa aaaaaatatg ctgcccaaag tcaggtaaaa atgggggatg
     1801 aacaagggcg cggcgcgggt cattccccat atgccgggca tcagctttgc ccccaaaacc
     1861 gatatgccca gccacagcat aatactcgcc caaaaccatt gcaacattcc tgcgcggaat
     1921 atcagaaaca gaaaaaccgc cgcatacagt aaggcagaca aaaaatccaa atgcagcaaa
     1981 atcatgaata cgccaactcg ccttcaaaca ccgcttccgc agggccggtc atcatcacat
     2041 cgccgccgca ggcccattcg atatataaag tcccgcccgg caaaatcacc tctaccgttt
     2101 tcccttcatc caacaacccc agacggatac ccgccaccac agccgcacac gcgcccgtac
     2161 cgcacgcttg ggtttcgccc acgccgcgct cgaacacgcg caaacgaatc gcggttcggc
     2221 tgacaatctg cataaagccg acgttgacgc gttcgggaaa ctgcctgtgc ggttcgataa
     2281 gcgaaccggt ttcgcgcacc ggcgcgcatt ctacgtcatc gaccacaatc accgcatggg
     2341 ggttgcccat attgacgcag ctgacaggct gaatgccgga ttcgagatgc accccgtaaa
     2401 tacaggcatc atccccctcg cccaattcgg ggacaaacgg tatttcagac ggcataaacc
     2461 tcggtttgcc catattgacc gtaaccatgc cgttatcgga caatttcgga aaaataacgc
     2521 catttgccgt ttcaacacaa atttctttct tatcggtcaa acccttgtct gcaacaaaac
     2581 gggcaaaaca acgcgctccg ttgccgcatt gcccgacctc gctgccgtcg gcattgaaaa
     2641 tacggtaacg gaaatccacg ccttcggttt ccgaacgccc gaccaccaaa agctggtcga
     2701 agcccacgcc tcggaagcgg tccgcccatg ccgcaatcgg cgcgtcctcg ggggtaaaat
     2761 cctgactgac cgcgtcaatc accataaaat cgttgcccaa accgtgcatt ttggtaaatt
     2821 tcaacgtgtt catattgttc cgaaatcccg tatcgtctgt tgcttccgaa tccgaaatcc
     2881 cccgcaccga agggaaatgc ttccatctcc cgccgcctga ggattaaaat cagccattat
     2941 actgtttgtt accccttaca agaaacacca tgttccgcat attactgttg cttttccctt
     3001 gtgcaatcct tgccgcctgc acccgaaaca taccggcaaa cccggccgtt tcaaattgcg
     3061 cgcatcttca aaaccgcacc atcctgtatt ttgacgcaca cggcaaaccc gccgacggcg
     3121 cagtaaaggg cggattttac aaaattatcg aaatacaggg tgcaaaccgc atccttattc
     3181 aagactttaa cagcacgggc aatgccctcg gcggatccta tgtgccggac tgccgcaaat
     3241 catccctttt cccgaaaaat atttgacttc aaactgccta tgaaccacga catcactttc
     3301 ctcaccctgt tcctactcgg tttcttcggc ggaacgcact gcatcggtat gtgcggcgga
     3361 ttaagcagcg cgtttgcgct ccaactcccc ccgcatatca accgcttttg gctgatcctg
     3421 ctgcttaaca caggacgggt aagcagctat acggcaatcg gcctgatact cggattaatc
     3481 ggacaggtcg gcgtttcact cgaccaaacc cgcgtcctgc agaatatttt atacacagcc
     3541 gccaacctcc tgctgctctt tttaggctta tacttgagcg gtatttcttc cttggcggca
     3601 aaaatcgaga aaatcggcaa accgatatgg cggaacctga acccgatact caaccggctg
     3661 ttacccataa aatccatacc cgcctgcctt gcggtcggaa tattatgggg ctggctgccg
     3721 tgcggactgg tttacagcgc gtcgctttac gcgctgggaa gcggtagtgc ggcaacgggc
     3781 gggttatata tgcttgcctt tgcactgggt acgctgccca atcttttggc aatcggcatt
     3841 ttttccctgc aactgaaaaa aatcatgcaa aaccgatata tccgcctgtg tacgggatta
     3901 tccgtatcat tatgggcatt atggaaactt gccgtcctgt ggctgtaagc cccattcatt
     3961 cccgataccc gtaaaaatac cgtctgaaac ctgttcagac ggcactttca ccctaagcgt
     4021 tactcggcgg cattttcctc acgggcaggc gcgtccagca gggctttgat ggacagacgg
     4081 acacggcctc tgtcgtccac ttccaatgct ttcacgttca ccacctgacc gacttgcagg
     4141 tagtcgccga cattgcgtac gcgctcgtgg gcgatttggc tgatgtgtac caaaccgtct
     4201 ttgcccggca tcacgctgac aatcgcgccg acattgttgt cgaggatttt caccacagtg
     4261 ccttcgtaca ctttgcccac ttccacttcg gcagtaatct gctcgatgcg ttttttcgcc
     4321 gcatcgccgg cttcttgagt ggttgcggca atggtaatcg taccgtcttc ggcaatattg
     4381 atttccgtac cggtttcagc ggtaatcgaa cggatggttt caccgccctt accgataact
     4441 tcgcggattt tgtcttggtt gattttcatc gtgaacaagc gtggcgcgtg tgcggacagc
     4501 tcttgcgggc ccgcaacggc ggctttcatc tgatccaaga tgtgcagacg cgcttctttg
     4561 gcctgtgcca aagcgatttg cataatttct ttggtaatgc cttggatttt gatgtccatt
     4621 tgcagcgcgg taacgccttc ggtcgtaccg gccactttaa agtccatatc gcccaagtgg
     4681 tcttcgtcgc ccaaaatgtc ggtcaggacg gcaaatttgt tgccttccag aatcagaccc
     4741 atcgcgatac cggcaacgtg tgctttcaaa ggcacgccgg cagacagcag gctcaggcag
     4801 ccgccgcaga cggaagccat agaggaagag ccgttggatt cggtaatttc ggagaccacg
     4861 cgcatggtgt agctgaaatc ttcaggtttc ggcaatacgg ccaacaatgc acgtttagcc
     4921 aaacggccgt gaccgatttc acggcgtttc ggtgcgccca tgcggcccac ttcgccggta
     4981 gagtacggcg gaaagttgta gtgcagcata aagcggtcgg tgtattcgcc ggacagcgcg
     5041 tcgatgattt gctcgtcgcg cgaagtaccc aaagttgcaa cggccaaagc ttgggtttcg
     5101 ccacgggtaa acaatgcaga accgtgcgtg cgcggcaata cgctggtttg gatgttcagc
     5161 ggacggacgg tgcgggtgtc gcggccgtcg atgcgcggtt ggccatccaa aatttggctg
     5221 cggacgacat cggcttccaa gtgtttgaaa atgcctttga tttcgttggc tgccaaagtg
     5281 tcggtttctt cggtaatcaa cgcttctttc acggcattcc aagcttcgtc caacttggca
     5341 gaacgcgctt gtttttgacg gattttgaac gcttctttaa tggtttcgcc ggcaatcccg
     5401 cggactttgg caaccagttc ctcattggtt tcaggtgctt tccaatccca aagttccgga
     5461 ttgacttcgt cggcaaattc attgattgca ttgatggcaa cctgcatttg atcgtggccg
     5521 taaaccaccg cgcccagcat cacgtcttcg ggcaggattt tggcttcgga ttccaccatc
     5581 aacacggctt ttgaagtacc ggcgaccacc aagtccaatt gcgatttagc caattcggct
     5641 ttagtcgggt tcaaaacgta cacgccgttt acataaccca cgcgtgccgc accaatcgga
     5701 ccggcaaacg gtacgccgct caacaccagc gcggcagatg cgcccaacat tgcaggaatg
     5761 tcggaatcga tttccggatc gacagacacc accatcgcta cgatttggat gtcgtggtag
     5821 aaaccttcag ggaataaagg acgaatcgga cggtcgatca gacggctggt caggatttct
     5881 ttttcgcttt gtttgccttc gcgtttgaag aagccgccgg ggattttgcc tgcggcgtaa
     5941 gtacgctcga ggtaatcgac ggtcaggggg aagaagtctt gaccttcttt cacttcttta
     6001 ttggtggtaa cggcaaccaa aacgacggtg tcgcccatag atactttaac ggcagcggcg
     6061 gcttggcggg cgatttcgcc ggtttccaaa gtaacggttt gattaccgta ttggaaggtc
     6121 ttaacgtgtt tattgaacat tattgttcct ttcaaaatac cgcactgcta aaacactaat
     6181 aatgcacact aaaatccgaa tgtgcatagt tagggtttca gaccgtgcgg caggttataa
     6241 acaagctttc agacggcact ccggacgctg aaaacagccc ccgattataa aggcaaccat
     6301 ccttaaaatc cagtattgat acaataaaaa ggccgtctga accatatttc tttcagacgg
     6361 ccttcaaact taaaaatcaa tcctgagtca gcaaagtcaa tgcttcctga tatttttcga
     6421 ctgttttttg aatcacattg gcaggcactt taggcgcagg ggcttttttg ttccaaccgc
     6481 tttgttccag ccagtcgcgg acaaattgtt tgtcaaaaga cggcggattt gtgccgactt
     6541 tatattggtc ggcaggccag aagcggctcg aatcgggggt caatacctca tccatcagcg
     6601 tcagcgtacc gtttgtatcc aaaccgaatt caaatttggt atcgcaaatg atgatgccgc
     6661 gcgatttggc atattccgcc gcttctgtat aaagctgaat cgctttggcg cgcacttccg
     6721 ccgccaattc ttttccgata atgcgttcgc attcttcaaa gctgatgttt tcatcgtgat
     6781 cgccgactgc ggctttggtt gagggcgtaa aaatcacttc aggcagttgt tgcgcttcct
     6841 gcataccttc aggcagttga ataccgcaaa ccgagccggt tttttgataa tctttccaac
     6901 cgctgcctgc cagataacca cgcacaatcg cctctacttt caccggagtg agctttttag
     6961 ccacgacggc gcgtttctct aaagctttgg cttcgttttc aggcaaaaca tcgtaaaccg
     7021 tttgaccggt aaagtggttg ggcataatat gcaccagttt tttaaaccaa aaattggaaa
     7081 tctgcgtcag aatctcccct ttgctcggaa tcgggtcgtc caaaatcaca tcgaatgcag
     7141 acaggcggtc ggaagcgacc atcagcatac gtttatcgtc gatttcatat aaatcgcgca
     7201 cttttccaaa atagatcttt accaaaccaa tctcactcat ttcgcccccc ctgaaaatat
     7261 cttgaaaata ccgacccgac acccgacagg tttgaatcac aaaccaatat tctagccgaa
     7321 gtcggcgcaa aacaataccc atggcacaaa aaaccaaccc gtcaaccgtc ggcaaagttt
     7381 tggcactata ataccgacag caagtcctac aatacacttt taccaaagga aatacctcat
     7441 gagaatccta ttgacaggct cgaaaagcca actggcacgc tgcctgcgcg accgtcttcc
     7501 ggaagactgg gaaaccattg cgacggattc cgcatcccta gacattaccg atgccgatgc
     7561 cgtctgcaac atggtcaaaa gtttccaacc cgacgctatt gtcaacacgg ctgcctatac
     7621 tgccgtcgac aaagcggaag gcaatgcggc agcggcattt gccgtcaatg cttccgccgt
     7681 ttacaacctt gccttggcag cacatcgcgc ccatgcccga ttcatccaca tctcaaccga
     7741 ctatatcttt gacggtaaag ggaaaaggcc ctatcaggaa agcgacttta ccaatccttc
     7801 caatgtatac ggacaatcca aaaccgcagg cgagctgctc gcactgtctg ccaatcccga
     7861 cagccttatc ctgcggactt cttggctgtt tagcgaatac ggggacaact ttgtccgcac
     7921 gatgctgaac cttgcgcggg aacgttcccc gctgtccgcc gtccacaacc aaatcggctg
     7981 cccgacctat gccggcgatt tagctgccgc catcatccgc cttttgcagc aaacgtcgcc
     8041 cgtgcgcggc atttaccact acgccggcgg caaatccgta tcctggtacg aatttgccca
     8101 acatattttc caagcggcat cgcaacagca gacatccttc cccgttcccg aattgactgc
     8161 cgtttcagac aaggaatatc cgaccgccgc ccccaggccc gcatacagca ttttggactg
     8221 ccgcaaaatc gaaaacgact tcagcatcaa accgtcagac tggcaaaaag cccttgcaca
     8281 ggtcgtttcc aagctgctct gatgccgccc gcctctccgt ttccgccgtc aagcaccgcc
     8341 ttggcggttt tctt
//
LOCUS       DAWEWP010000082         8318 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWEWP010000082 DAWEWP010000000
VERSION     DAWEWP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.082"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(337..1722)
                     /gene="pntB"
                     /locus_tag="WGN53_001328"
     CDS             complement(337..1722)
                     /gene="pntB"
                     /locus_tag="WGN53_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ0140318.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKGANSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1788..2171)
                     /locus_tag="WGN53_001329"
     CDS             complement(1788..2171)
                     /locus_tag="WGN53_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ0140319.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAAQSTINTLACLFILFRLAFIWCYIA
                     DKAAMRSLMWAGGFACTVGLFVAAA"
     gene            complement(2175..3716)
                     /locus_tag="WGN53_001330"
     CDS             complement(2175..3716)
                     /locus_tag="WGN53_001330"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ0140320.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESSAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLN
                     MFKKG"
     gene            4023..4856
                     /gene="serB"
                     /locus_tag="WGN53_001331"
     CDS             4023..4856
                     /gene="serB"
                     /locus_tag="WGN53_001331"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ0140321.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SLLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKAQAAADACINFGGLERVRGLF
                     R"
     gene            4922..6145
                     /locus_tag="WGN53_001332"
     CDS             4922..6145
                     /locus_tag="WGN53_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0140322.1"
                     /translation="MGRKKLWFALVAAGVIGGLVGIVLTELMHFIQHTAYGYGADGVY
                     TSFREGVAQASGMRRVAVLTLCGAVAGVGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFASAGGKRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVYFRRSAEKFPFLKRDNPRIIPLAIALFALIGAIAVWFP
                     EILGNGKAGNQLTFGGLTDWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLNMPLTAAVFILELTYAPAALLMPLCT
                     GMAGAVWTARKMGCK"
     gene            6310..7890
                     /gene="purH"
                     /locus_tag="WGN53_001333"
     CDS             6310..7890
                     /gene="purH"
                     /locus_tag="WGN53_001333"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ0140323.1"
                     /translation="MPSIKRALISLSDKTGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     LNRADLKVVSKRQPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDANLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1859 a         2268 c         2348 g         1843 t
ORIGIN      
        1 acaaagccaa cgagtttttg ggtatgaccc gcctgccgac attcttatgt aacgatgtgg
       61 ttaaaaatcc gcaagtggaa aaatacttgg cagattatca ggcacacttg gaaaaagtgt
      121 tcggctaatt aaaaatccat cttcaacacg gagatggatt ttgtttgttt cgttgatttt
      181 gtgtcagttt cagatgtagg tgcttattcg gacgggccgt ctgaaaatgt ttgccccaat
      241 gcaaaaaaaa tcactgcaaa ccttcataaa cggggtttgc agtgattttt tcaaatcaaa
      301 cagattgaaa acctgcgccg aattgttcag acggcattat tttttcagtt cggacagaat
      361 gtcatccacg gttttcttcg catctccgaa acacattacg ctgttttcgt tgaagaacag
      421 cgggttttgt acacctgcgt agccggtatt catcgagcgt ttgaagacga cgacttcttt
      481 tgccttccat acttccaaca cgggcatacc cgcaatcggg ctgttcgggt cggtttgggc
      541 ggcaggattg acggtatcgt tcgcaccaat caccaagacc acatcggttt cggggaagtc
      601 gtcgttgatt tcgtccattt ccaacacgat gtcgtagggg actttggctt cggcaagcag
      661 tacgttcata tgaccgggca ggcggccggc gacggggtgg atgccgaagc gcacttctgt
      721 gccgttttta cgcaaaagct cggtgatttc ggcaacgggg tattgcgctt gtgcgactgc
      781 cataccgtag cccggggtaa tgatgacact gtttgcgcct ttcagcattt cggcaacatc
      841 ggcagctttg acttctcggt attcccctat ctcttggctg ccggaagatg atgtgccgct
      901 gtcgctgccg aaaccgccgg cgattaccga gacaaacgac cggttcatgg ctttgcacat
      961 aatgtaggac agaatcgcgc cgcttgagcc gaccaacgcg ccggtaacga tgagcaggtc
     1021 gttggagagc ataaagcctg ccgctgcggc cgcccagccg gagtaggagt tgagcatgga
     1081 cacgaccacg ggcatatctg cgccgccgat ggaggcaacc aagtgccagc cgaatgcgag
     1141 ggcaatcagg gtcataatca gcaggatgaa gccgctgccg tcaatgccga cgaatacgag
     1201 cagcaacaca aacgatacgc caagtgccag tgcgttgagc ttgtgtttgg cgggcagttg
     1261 cagcgggctg ctgctgattt tgccgttgag tttgccgaat gcgaccagcg agccggtaaa
     1321 ggttaccgcg ccgatgaaga tgcctaaata cacttcgacc agatggatgg tgtgcatatc
     1381 gtgcgaaacg ttgcccggcg cgatatagct gttgaagccg accaaaaccg ccgctaggcc
     1441 gacgaagctg tgcagcaggg caatcagttc gggcatttcg gtcatttcca cctttttggc
     1501 tttgtagatg ccgattgccg cgccgatgag catggcgatg atgatccagc ccagtccgtg
     1561 ggtattgtcg gaaaaaacag ttacaaaaag ggcgaccgcc ataccggcga taccggaata
     1621 gcagccctgt ttggcggttt cctgtttgga cagccccgcc agtgagaaga tgaataaaat
     1681 tgcggcaacg atatacgccg ctgttacgag tcctgaagac atggaaattc tccgattttc
     1741 gatgatttgt tttcaatgcc gtccgaactg ttcagacggt atctgtttca agcagccgcg
     1801 acaaacagcc cgacggtgca ggcaaatccg cctgcccaca tcagtgagcg catagcggct
     1861 ttgtcggcga tatagcacca gataaaggcg aggcggaaca ggatgaacag gcaggcaagc
     1921 gtgttgatgg tcgattgcgc cgcattgccg gttgcgtgtg ccgtcaaaac ggcggcggca
     1981 aacggtgcaa aggcttcaaa accgttttgc tgcgcggcgt gggcacgggc ggctgtgcct
     2041 tgcgtgtgcg ccagaaaatc gcgcggattg tggttgtctt taaaccggaa tccgcccgct
     2101 tttttggcat atgccgcaca aaaaagcggc aataggcagg caatcaggat gcaccaatag
     2161 gcgaaagtca tggcttaccc tttcttaaac atattcagca tacgccgtgt aaccgcaaag
     2221 ccgccgaaga tgttgatgcc ggcaatcagg atggcaacaa acgacagcag cgaaacaaag
     2281 ccgttgccct gaccgatttg cagcagcgcg ccgacgacga tgatgccgga gatggcgttg
     2341 gtcacggaca tcagcggcgt atgcagcgag tggctgacat tccaaacgac gtagtacccg
     2401 atgacgcagg caaggacgaa cacgataaag tggttcagga atgctgcggg tgcgaccgcg
     2461 ccgacccaca gcaccaatac ggcggcgatg acggcgggcg cgagtttttt ccacagggaa
     2521 acaggtttcg gctcgggctt ggcggcaggc gcggcttttt cagacggcgt ttgctgcggc
     2581 tgggcggaaa cttgaatcgg cggaggcggg aaggtgattt cgccgtcgtg ggtaacggtc
     2641 atgttgcgga taatcacgtc ttcgaagtcc aacgtgattt cgccgtcttt gttcgggctt
     2701 aacagcttgg tcaggttgac caagttggtg gcgtaaagct gggaagactg tccggcaagg
     2761 cggtttgcca tgtcggtgta gccgatgatt ttcacgccgt tgccggttac ggacaattcg
     2821 cccgggcggg tgagttcgca gttgccgccc gtcgccgccg ccaaatcgac gatgacggag
     2881 ccggatttca tgctttccac catttctttg gtaatcagct tgggcgcggg tttgcccgga
     2941 atggcggcgg tggtgatgat gatgtccact tctttcgcct gctcggcaaa gagcttcatc
     3001 tcggcggcga taaattcgtc gctcatcact ttggcgtagc cgtctccgct gccgcccgat
     3061 tcttgtggga agtcgagttt caggaacttg ccgcccatcg attcgatttg ttccgccact
     3121 tccaagcggg tatcgaacgc gcgtaccact gcgcctagtg aatttgccgt accgattgcc
     3181 gccaaacctg ccacacctgc accaatcacc aaaacctgcg caggcggcac tttgccggcg
     3241 gcagtaattt gaccggtgaa gaaacggccg aaggcgttgg cggcttcaat cacggcgcgg
     3301 tagccgctga tgtttgccat cgaagacaaa gcgtccaaag cctgcgcgcg ggaaatacgg
     3361 ggcaccatgt ccatcgccag cgcattgact ttcttggcgc gcaaggcttc gaccaaagcc
     3421 tcgttttggc gcggccacag gaagctgacg atggtttgac cttcgttcaa aagcggcagc
     3481 tcgccttcgg acggcgcgtt aaccttataa atcaaagggc aggcccaaac cgccgctttg
     3541 tcggcaacgg ttgcgcctgc tgtttggtaa gcggcatcgt ccaaacttgc cgccaaacct
     3601 gcactgcttt cgacaacggt ttcaaagccc agtttgccca gcagggcgac ggtggcgggc
     3661 gtgcaggcga cgcgggtttc gccggataat gactcgcgtg ggataccgat tttcatctct
     3721 gaatcctttt tcgggttgtt tactttttcg ggttgtttat atgtatcgtg ggttaaattt
     3781 aaatcggggc agggcggagc aacgccgtac cagtttaaaa ccgattcact tcaaatgtta
     3841 atatatttta gataatcccc ttataacgaa ttttcatcag gctggcaata gttgcggcat
     3901 tttcccgtgt tgtccgacac atactgccgt ttgcgtttga agtcattttc cgattgcagt
     3961 cccgcccgcc gtaacattct ttgccgtttc actatataat ccgcattttt tgagccgccg
     4021 ttatgccgca agccctcgtc ctccaatttc cctccgccga agccctgcct tccgacttcc
     4081 cctcccgcct gcccgaacct gattgcgccg atgaaaagcg tatgcgtttt attgttgaag
     4141 aaggattttc tttaagcgaa aaagacgcgg cgttgcttgg cagccgtcaa atcgaccacg
     4201 ccgttttgcc ggatatggat ttcgacgaac tcggtttgat tgtcagcgat atggattcga
     4261 cgctgattac catcgaatgc gtcgatgaaa ttgcggcagg cgtgggttta aaagacaaag
     4321 tagcggaaat taccgagcgt tcgatgcgcg gcgaactcga tttcgagcag tctttgcgca
     4381 gccgtgttgc gctgttggca ggattggacg aacagatttt ggcggacgtt tatgaaaacg
     4441 ttttgaagct ctcgcccggt gcggaatttt tgttggacga atgcaaaagg cacaatgtga
     4501 aattcctgct ggtgtcgggc ggcttcacgt tttttaccga aaggctgcaa caacgcctcg
     4561 gcttcgaata ccaacacgcc aacgttttgg aaattgaaaa cggcaggctg acgggtcgtc
     4621 tgaaaggcag aatcatcgac gcgcaggcaa aggcggattt attgcgcgaa taccgcagcc
     4681 tcctcggatt gcagccgcat caggtgttgg cgatgggcga cggtgcgaac gatattccga
     4741 tgctcaaaga agcgggcata ggcgtggctt accgtgccaa accgaaagcg caggcagccg
     4801 ccgatgcctg tatcaacttc ggcggtttgg agcgtgtacg cggcctgttc aggtaggcgg
     4861 ataggaaacg gatgccgtct gaaaaccgtg ttcagacggc atttgaacgg caggaacgac
     4921 agtgggacgc aagaaacttt ggtttgccct ggtagcagca ggcgttatcg gcggtctggt
     4981 cggcattgtg ctgacggaac tgatgcactt catacagcat accgcctacg gttatggcgc
     5041 ggacggcgtg tacacttcgt tccgcgaagg cgtggcacag gcttccggta tgcggcgcgt
     5101 tgccgtgctg acgctgtgcg gcgcggtcgc aggagtcggc tggtggctgc tcaaacgttt
     5161 cggcaagccg caaatcgaaa tcaaagccgc cttgaaacag ccgttgcagg ggctgccgtt
     5221 tctgacgacg gttttccatg ttctgctgca aatcataacg gtcggactcg gttcgccgct
     5281 cggacgggaa gtcgcgccgc gcgaaatgac tgccgcgttt gcttccgcag gcggcaaacg
     5341 cttgggtttg gatgaaggcg aaatgcgcct cttgattgcg tgtgcctcgg gcgcgggctt
     5401 ggcggcggtg tataatgtgc cgcttgcctc cacacttttc atcctcgaag cgatgttggg
     5461 cgtgtggacg cagcaggcgg ttgctgccgc gttgctgact tcggtcattg ccaccgctgt
     5521 ggcgcgtatc ggtttgggcg acgttcagca gtatcatcct gccaaccttg ccgtcaacac
     5581 atcattgctt tggttttccg ccgtcatcgg cccgatactg ggcgcaacgg cggtatattt
     5641 ccggcgcagt gccgaaaagt tccccttcct caagcgcgac aatccgagaa ttattccttt
     5701 ggcgattgct ttgtttgcac tcatcggcgc gattgccgtt tggtttcccg aaattttggg
     5761 caacggcaaa gcaggcaacc aactgacctt tggcggattg accgactggc aacacagcct
     5821 tgagctgacc gccgtcaaat ggctggttgt cttgatggcg ttggcggcag gcgcatacgg
     5881 cggactgatt acgccgtcta tgatgctcgg cagcaccatt tcttttgcag ccgccgccgc
     5941 gtggaacagc gttttccccg aaatgccgtc tgaaagcgcg gcagtcgtcg gcgccgccgt
     6001 tttcctcggc gtttccctca atatgccgct gaccgccgcc gtctttattt tggaactcac
     6061 ctacgccccc gccgctttgt tgatgccttt atgtacgggt atggcgggcg cggtatggac
     6121 ggcaaggaaa atgggatgta aataaccgaa aggcaaaatg ccgtctgaaa ccggttttca
     6181 gacggcattc ctttggggat tgtcaacaat atctgcaaaa acctactgcc agaaagtgga
     6241 aaatagcgga taataccgcc ccgacaacat taccgtttcc catcccaacg tcagaaaaag
     6301 gaaatcccta tgccttccat caaacgcgcc ctgatcagcc tgtccgacaa gacgggcgca
     6361 gtcgaatttg cccaaaccct gaccaagctc ggtgtcgaaa tcctctctac cggcggcacg
     6421 gcgaagctgc ttgccgatgc gggcgttccc gtgattgaag ttgccgacta caccggtttc
     6481 cccgaaatgc tcgacggccg cgtgaaaacc ctgcatccga aaatccacgg cggtattttg
     6541 ggccggcgcg atttggacga acacgtcgcc aagatggaag aacacggcat cggcaacatc
     6601 gacctcgtgt gtgtcaacct ctaccccttc gccgccacca tcgccaaacc aaactgcacg
     6661 ctggaagacg cgattgaaaa catcgacatc ggcggcccga ccatggtgcg ctccgccgcg
     6721 aaaaactgga aacacgtcgc catcgttacc gacacagccg atttcccggc catcgccgcc
     6781 gaactcgaag ccaacaatgg cgcattgagc gacaaaaccc gcttcaacct gtcgcgcaag
     6841 gcgttcagcc acaccgccca atacgacggc atgatttcca actacctgac ctcactttca
     6901 gacgacgttt tgagcggaga gcctgaaatc ggcgaatttc caagccagtt caaccaaagc
     6961 tggattaaag tgcaagacat gcgctatggc gaaaacccgc atcagcgcgc cgcgttctac
     7021 cgcgatattg accccgccgc aggcagcctt tccgcctaca aacaactgca aggcaaagaa
     7081 ttgtcttaca acaacatcgc cgatgccgat gccgcatggg aagccgtcaa atcctttgac
     7141 gcgccagcct gcgtgatcgt caaacacgcc aatccgtgcg gcgtcgccgt tgcagccgat
     7201 acattgaccg cctacaaact cgcctacgtc accgacacca ccagcgcgtt cggcggcatc
     7261 atcgccttca accgcgaagt ggacggcgaa accgtcaaac agattaccga caaccagttt
     7321 atggaagtct tgatggcgcc gaagtttacc gccgaagccc tcgaaatcgc cgccgccaag
     7381 aaaaacgtgc gcgtattgga agtgccgctt gaggcaggcg caaaccgctt cgaactcaaa
     7441 cgcgtcggcg gcggactgtt ggtacaaacc cctgatattc accgcctcaa ccgcgccgat
     7501 ttgaaagtcg tctccaaacg ccaaccaacc gagcaagaat ggaacgacct gatgtttgtt
     7561 tggaacgtcg caaaatacgt caaatccaac gccatcgtct tcggcaaagg cggtcaaacc
     7621 tacggcatcg gcgcaggcca aatgagccgc gtggacagca cccgcatcgc cgcccgcaaa
     7681 gcgcaagatg ccaatctcga cctcaacggc gcatgtgccg cttccgatgc tttcttcccc
     7741 ttccgcgacg gcgtggacgt gattgccgag cagggcatca aagccatcat ccatccggca
     7801 ggttcgatgc gcgatcagga agtcttcgac gcagcggacg aacacggcat cgccatggtc
     7861 gtaaccgacg tgcgccactt ccgccattaa cgcagatgaa caaggcaatg ccgtctgaag
     7921 ggctttcaga cggcattttg cgctattttg tgaaggtagg gatgacggtt cgggtattcc
     7981 tgacagggtg gattttcaag gtgttgtata gggtgtagga ggattcgtaa aaggtgggat
     8041 gcagggtgtg cttcagcccg ctgcatcaaa aatttttgga gaatcggcgg gagtcggcgg
     8101 ttttggtttc ggcggggacg gtggaaatgg gtaacattga cggaatcgac ggaagcggtg
     8161 ggcttcagcc caccgcttgt atattggaat caccgtatca tagcgccacc cgcgcccacc
     8221 caaggcacac aaccgttgcg tagctcaggg agcggcaggg caacccatcg acacaaccgg
     8281 acagttgccg gacaacacaa ccgaatgcaa ggcaggtt
//
LOCUS       DAWEWP010000083         8275 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWEWP010000083 DAWEWP010000000
VERSION     DAWEWP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.083"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(32..490)
                     /locus_tag="WGN53_001334"
     CDS             complement(32..490)
                     /locus_tag="WGN53_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140324.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGTEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(1054..1257)
                     /locus_tag="WGN53_001335"
     CDS             complement(1054..1257)
                     /locus_tag="WGN53_001335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140325.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
     gene            complement(1821..2756)
                     /locus_tag="WGN53_001336"
     CDS             complement(1821..2756)
                     /locus_tag="WGN53_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0140326.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESIDYVDERKGVP
                     LAAMKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(2759..3379)
                     /locus_tag="WGN53_001337"
     CDS             complement(2759..3379)
                     /locus_tag="WGN53_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140327.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQIELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSREWIKQVVKSLPD
                     GSPAKAVVLKANQNGKLKTAIAGVDRQTGKAVILSVKVPSKTNIRR"
     gene            complement(3458..3883)
                     /locus_tag="WGN53_001338"
                     /pseudo
     CDS             complement(3458..3883)
                     /locus_tag="WGN53_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(3931..4203)
                     /locus_tag="WGN53_001339"
     CDS             complement(3931..4203)
                     /locus_tag="WGN53_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140328.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            complement(4391..4879)
                     /locus_tag="WGN53_001340"
     CDS             complement(4391..4879)
                     /locus_tag="WGN53_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140329.1"
                     /translation="MKKDIFYCEQWSYGYKRLHKPFSEKQAEEKHIKGELYSAVIGSA
                     TQPEYVITLREEVGFFSVDFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLDSH
                     DLAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYSQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(4876..7377)
                     /locus_tag="WGN53_001341"
     CDS             complement(4876..7377)
                     /locus_tag="WGN53_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0140330.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRIILSAADIAAENGK
                     IKLQSYGDQSYYAGQGELYTLERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATVFDAPKGSINIEAGRKLTLYAVEELNYDKLDSHKKRKFLGITYD
                     KVHDTSTQQMQTALPSRVVAESANLQSGWDTKLQGTQFETTLGGAVIRAGVGDQARAD
                     AKIILEGIKSSIHTETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGMTPAAAA
                     VVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATTGVAAGTS
                     AAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAAVTAGVLQ
                     GISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNLGDAALGA
                     IVSTVHGEVASKIKFNLSEDYITHKIAHAIAGCAAAVANKGKCRDGAIGAAVGEMVGE
                     TLLDGRDVGKLSPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVENNSLLARR
                     RVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAIMRNPREPWTRHDVQTYRQ
                     YYNQLRESRGIPVLPIYRMRINNGNEFNRIMSSKYPYNELYVANPKSVTGHFRVDSYN
                     PATREIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAVISNVPSTPASLRGRKLTGKL
                     ILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
     gene            complement(7627..8010)
                     /locus_tag="WGN53_001342"
     CDS             complement(7627..8010)
                     /locus_tag="WGN53_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140331.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(8015..>8275)
                     /locus_tag="WGN53_001343"
     CDS             complement(8015..>8275)
                     /locus_tag="WGN53_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEZ0140332.1"
                     /translation="PETKIFKPISVDKYHNIASPPRGTLRNIDGEYKLLETIAQQLGN
                     NRNVSGRIDLFTELKACQSCSNVILEFRNRYPNIQLNIFTGK"
BASE COUNT         2169 a         1792 c         1622 g         2692 t
ORIGIN      
        1 atcgattccg ttgtattttc gcaagacacg tttagtctag agtctgtata ttacatttat
       61 ttttagggtc tgctagccaa tttcttgttc ccttcattat tttatcttct gaaagaaaat
      121 tatttttttc catgctatta atattaatga tatgattttt atttaaaata atttttccat
      181 ataaaatttc cggtttgtaa tagcaagagt ctgaagcaaa tccttgcccg tcaatataag
      241 aaaaataaat gttatcatcc tccttataat attctaaaac caatccggat attattttat
      301 caaaattccc ttcagttcca tctttcatga ctaaaatata attagcaggc caacctttat
      361 cgtaaacata ttcaaactga tagttcccga aactctcgat atccgaacta aaaaagaaga
      421 aaagcaaatt aatagccaat acaaaaacaa ataagaacaa aaatagcaaa attttcaact
      481 tagttaacaa tatttacctc tcctttaaat tcaatcctga aaggtacccc ttaccctggg
      541 ctaccaatta tagtttccat atttctaaat attgttttta cattactttt ttctcccccc
      601 aaaggaattc attttaaaat catgcttttc aggtgctaat cgatacttac cattaacatc
      661 tttaatcaca gatatatttc catgtatagc ccaacgtgaa aaatctgagt attatataca
      721 gttatacctt actgaaacaa agctagctgt acatatctgt accatatttt tactacaaac
      781 aaaaaatatt tataattatt aaggataaga taaaaacccc caacgccaga ttaattctta
      841 ataatttcaa ataaaaaata tttgagattt tttcttttcc aaataatata ttgaaatatt
      901 taaagttaag gtagcttgat ttaagtagaa aatcaaaaaa accattaatt aatttttctt
      961 ctttaaatat tttatatgat attataaaat taaataacat tgcgaaaaca tatgaaatca
     1021 tcaaaatacc aaatatatag tgggaatgca ttattattta gctccttctt ttggctctga
     1081 caaattgaca gtccattgat atccaatgct tgcatttgca attgctgacg cacctgcccc
     1141 tgttttccct gttagtatca atggttcttt tcttgcaaga tgtgcacctg cacaaccacc
     1201 taatactaaa ccaccaccta ttgtaaatcc ttcctgttgt atctctaatg ataacatttt
     1261 cttcttgtgc cgcccttaat acagattgtg gaattggatt gacctgagca ggaacttcta
     1321 aaataagttt tccttcaagc tgtcttcctc ttagcagatt aggtgtactt ggaacattgg
     1381 aaatgacaaa gtgagtgcgc aaagccacct gaaggcgatg tgtttgaacc tgttgaaagc
     1441 ggccaacagg ctaaatgcgc ccgctgccgc ctaaaaggca gcccggatgc ctgattatcg
     1501 ggtgtccgag gaggattaag ggggtatttg ggtaaaatta ggaggtattt ggtacgaaaa
     1561 cagccgaaat cctgtgtttg ggtttcggat gtcggggaag ggcttttttg caaaggtctc
     1621 aactcatgtt attgcaaaga tctcatgcct tgttttgcca gcaggttgag ttcttctccg
     1681 gcaatcagcg tggtaaagcc ccgccacaac acagatctat gtactgtgga agggcttcaa
     1741 tttatagctt tcaaaaatct ttcagcttta agacggccta aagttgtaat ctataaaaca
     1801 ggagctcaga gttttacggc tcactctcgc ttcgcgttac caaataccag cttatcggtt
     1861 ttgaacgtat agtaatctat ccaatcctgc agatattcat acgggtaaga caagtcatac
     1921 tgtttcataa aatcgaagat gtcttgatat tcgtctgccg gcagcggatc gtaaacaatc
     1981 aaatccctcg gtgcgatttc ttgatctatg cccaaatcga aaccgtgcat ggatgcgatt
     2041 ttggcgtagg taacgatttg cggctgcagg tagaaatcga aataggacaa tgtttctttg
     2101 gcagccatac gcgcggtttt tttatcgaaa agcggctcta aggcggcctt catcgcctcg
     2161 gcatcctgtt cggcgaaagc gaggaagaag cggtaatcgt acaaccgctt ttgcagccat
     2221 ttgctagggg tgggatgcgc caagactttc tcgctacgtt gtttcaaccg atcaagctgc
     2281 ttaccctcca ccatcagcag agtattgtaa atcatatgac ggttgaggtc gtatcggttt
     2341 acgaaggctt ctgtatcgtt ggcgatgttg tcgatattgc gcaccaaaaa ctcgcgcagc
     2401 tgcgggctgt cgctcatcag catcagaaac atcggatctt gaatgttgag catgtcgcag
     2461 ggaaagaaga agggttcggg gtcttcatca tctacactct gcaaaatacc caactttcct
     2521 gcaacataag catattgctt gaatttctta agatcatgtt caaatagata ggcatgggaa
     2581 gccgcagctt cagtgtacat taaaatgtat ttcatcgctg caagcggaac gccttttctt
     2641 tcatctacat aatctataga ttcggggcaa ccgctattga aattagcagt attgcctatg
     2701 attacattag taatatgttc ataccatttt tgggtggtca tcatattgtg ccccattgtt
     2761 atctccttat attggtttta gaaggaactt tgacagaaag aataacggcc ttacctgttt
     2821 gacgatcaac gcctgctatt gccgttttta atttgccgtt ctgatttgct tttaagacaa
     2881 ctgccttagc aggactacca tcaggtaaac tttttacaac ttgtttaatc cattcacgac
     2941 tcatctgcgt atatccaccc gcaccattcg gattcagctg taccgtaccg ttcctaatct
     3001 gcttactttc tacaatcaaa acaacactac catcggcagc ctgccataca tgatcaaaac
     3061 cgttatttcc gccgtatttg ccgcccgaaa gcactctgaa gccattttgt ttagctaaag
     3121 agtcaaataa ctgctcggtc attttacctg tcgtgtccct tccttcgcca atagctttca
     3181 gcaattgttt gtcagttgct gcagtaaccc ttaattcaat ttgaccgatt ttgattttgg
     3241 ttttactgac atagccctgt gcagtggcaa cggttgctgc cttgtccaag gcttttttca
     3301 tgccttgtaa attttttgcc gctttcgcta ctttataggc ctgtatcgat tcaccgatac
     3361 ccggaaccac acccagcaaa gcaaacaggt gatcggcagc ggtctgtgct tgtacaaaac
     3421 tctgtatatc accgataatt ggaataaatc ctgtctgaag aagcaaaact tctcgatatt
     3481 cttcaggatt ttcacgtatc catttttgtt gttccttttg aaattcatca cactttgcac
     3541 caacgcatct aggatacaaa gtattattct ccaccgctat ctgtgccgca ttcgccgctg
     3601 tattcacatc cccgcctaca acggcagacg cagtgccggc aaccagtttt gaataggcag
     3661 taatcttcgc tttagatttt ttgatttcgg tcgcactcat acgactgaaa tcagtattct
     3721 taaccaaagc ctcaccaaca atctcaccga ctgcagcgcc tatcgcgcca tcctgacact
     3781 tgcccttatt cgccgccgct gccgcacagc ccgctatggc atgggcaatc ttgtgggtgg
     3841 tatatcgctg ccagccctct ctgttgataa tcttgcgtat gattacgccg tttgatggct
     3901 aatcgtacct acgcaggctt aaaatttcca tcaatccata tcatttgagt tttcatcttc
     3961 ataatatgga tgatcaaaca aatctccttc ccaaatacat tcactcgtta taccttggga
     4021 ttccatgaaa ggaacataag aataaaaatc tttttcatat tctccataaa atatattcat
     4081 acctttgagc catactgcat cgcattcatc acacagaata atttcatcat gaagatattt
     4141 aggtttcgaa tggtagagat agccttgttg acaagcagga cacattttca ttctcttatt
     4201 catgattact ccatataatc gatttatttt tgtgggtaag cagtagtgat ttgattgctg
     4261 cctttagtct gtacaacaat acgtatatag caatctgata atttcctggt ttttatcagc
     4321 tttatcctca gattccaaac ccaataataa aagccgtttg aaattttttt cagacggcct
     4381 taaaacttaa ttaaataatg tccggattta acaccaaatc atattcgcca aactttggaa
     4441 atagaattac cttatctttt tgggaatata attctgtttt ttcatatcgc tcattattaa
     4501 taaaatcctc tttcaaaaca tagcaatcat ccgtattttc attgaagaaa taagtatatc
     4561 cttctgctaa gtcatgagag tctaaagtta tataatctct ccatacagcc atagaaagaa
     4621 aataataatt cgaattggaa tatttttgaa attgatgggt taaataatcc cttccaaatt
     4681 tatcgaaaaa atctaccgaa aaaaaaccta cttcctctcg caaggtaatt acatattcag
     4741 gttgtgtcgc cgaacctatt acggcactat ataattcccc tttaatatgt ttttcttcag
     4801 cttgtttctc agaaaaaggc ttatgaagtc tcttataacc ataagaccac tgctcacaat
     4861 aaaaaatatc tttcttcatt tgtaaatcct tcctgttgta tctctaatga taacattttc
     4921 ttcttgtgcc gcccttaata cagattgtgg aattggattg acctgagcag gaacttctaa
     4981 aataagtttt cctgtaagtt ttcttcctct tagcgaagca ggtgtacttg gaacattgga
     5041 aatgacagca ccagggctat attttctaac agcctccttg atataaccaa tccccgtact
     5101 ttcttggatt tgagaaaatt gggtaaattt tcttgaaata atttcccttg ttgcaggatt
     5161 atacgaatct accctaaaat gtcccgttac cgatttagga ttggctacat aaagctcatt
     5221 ataagggtat tttgatgaca tgatacggtt aaattcattg ccgttgttta tcctcattct
     5281 ataaattggg agaacaggaa tgcctctgga ttcccttaat tgattataat attgcctgta
     5341 tgtttgtaca tcatgtcttg tccacggctc tctaggattc ctcataatag caatcccgtt
     5401 aaatttcgga tccagccttc ggatttgatt ggtaatggcc tggatttcaa gaatggcata
     5461 ttcatgttcc aattcttgtc gcggagtcca acgtatattt accctcctgc gagctaaaag
     5521 actattattc tccactgcca cagtagccgc attcgccgcc gtattcacat cccctttaac
     5581 caatgccact gcgctgcctg cgataatctg cgagtaggct atgacttttt ggcgttcttg
     5641 gggtgacagt ttgcctacat cgcgtccgtc caacagggtt tctcccacca tctcgccgac
     5701 tgccgcgccg attgcgccgt cccgacattt gcctttattt gctaccgctg ccgcacagcc
     5761 cgctatggca tgggcaatct tgtgggtaat gtagtcttcg ctgagattaa atttgatttt
     5821 gctcgctact tctccgtgta cggtactgac tatcgcaccc agtgcggcat cgcccaagtt
     5881 gtctttgagg ctgccgccgt tgatggcggt atggacactt gcggcagcgg tgctgttgat
     5941 caggttagcg gtcagtttgc ctgctgcggg actgtgaaaa tgtttgctga cggcttcggc
     6001 tgcttgggtg ttcagcccgc ttatgccctg cagtacgcct gcggttacgg cggcggtggc
     6061 ggcctgtctg acggtgctgc ttttgcccag ttctttcagg gtatggttta tgtctccttt
     6121 gttgttgatg agggaaactg cggcttggct ggcgagactg gccagtgcgg ctttgcctgc
     6181 ggctgtggtg atagctgcag ctgatgtgcc tgctgctact ccggttgtgg ctgccgttcc
     6241 tgctgctact ccagttccgg ctgccgttcc tgctgcggct cctcccgctc ctgcgcctgc
     6301 cgcgcccgcc gttccggcgg ctgccggggc ggacagtgca ccgtaggtca atacggttac
     6361 gacgataacg acgacagctg ctgctgcggg tgtcatgccc tcctgtttgt agtcccattt
     6421 atcgtaagca agctgcacct gattccagtt gatgtttttc gcaacttgaa gttgtttcaa
     6481 ataagcatac tcgggctgct tggtgagggt ttcgatttgg gttttcagat tgcctttcgg
     6541 aatgtcgaca atgtaaccgc cgggagcaga gagtacgggc gcaacggggc cggtgaaact
     6601 tggcagttgc agggtttcga tgttgctgcc gcgtcctgcc tgtttttgcc agagggcgga
     6661 tttgctgctg cttacggttt ctgtgtggat gctgcttttg atcccttcga ggataatctt
     6721 ggcatcggcc cgtgcctggt cgcctacgcc tgcacggatg accgcgccgc ccagcgtggt
     6781 ttcaaactgc gtaccttgta acttggtatc ccaacctgat tgcagattag ctgattcagc
     6841 cactacacgg cttggtaagg cagtttgcat ctgttgggta gaggtatcat gtaccttatc
     6901 ataagtaatg ccgagaaatt tgcgtttctt atgactgtct agtttgtcgt agttgagctc
     6961 ttctacggca tagagtgtca atttccgccc ggcttcgatg ttaatgctgc ctttgggggc
     7021 atcgaatacg gtggcgtagg cgtcgatgct gccaccagat ttgatgtcga taccttggga
     7081 tgcgctgagg tttactgcgt cgggcttggc gtttttgtgt tctttgactt cggtaatgtg
     7141 tttgcggttg taccatttgc cggttttgta gctgcggcgt tctaaggtat agagttcgcc
     7201 ctgcccggcg taataggatt ggtcgccgta ggattgcagt ttgattttgc cgttttctgc
     7261 ggctatatcg gcggcactca aaatgatgcg gctgttctca ttggcatacg gtgcgctaat
     7321 gctcacaccg gttttacccg aaagttctgc agcaagcgta gggggtgtct tcagcacgca
     7381 cgcgtctggg cagtatatag ggaatctgaa tatttacttg aataacaaat gccgtctgaa
     7441 aaattgtgag cttttcagac ggcattgagc cgtaaatcat ggaacgcgtg cgtgctgaag
     7501 cacacacctt acgcatggat tttaggtttc atgcaggcta cagcttgctg cggcggcggt
     7561 ataaaagtat ttttagtgat ttaccgtttt tggtaatttt gcccgacggg gttaaaaata
     7621 cagttgctac tcttgctgca tgatattggc aagatttaat ttaaatgttt gttgaatctt
     7681 gtttacatcc ttagcaaaaa tatgatagtc actcgaagaa tagatagatt ttattctatc
     7741 caaattagtt tgttgataac attttttcat ttccgcgatg aaaaaattat cttttcctct
     7801 ttcattgaga cagttatttt ctaacagata gattgcccat gctgtaaaaa taaatgtact
     7861 atcttccgga tattcagata aataaatttc gaattcaatt atagaatatt cagctgcttc
     7921 aattaatgtt atatctaaat ctctgacaaa tttttccgtt atatttttaa ataatttctt
     7981 gaattctata taatttttta ttttttccat gattctattt tcctgtaaaa atatttaatt
     8041 gaatatttgg atagcgattt ctaaactcta aaataacatt gctgcaagat tgacaggcct
     8101 ttaattctgt aaatagatca attctacctg atacattacg attatttccg agttgctgtg
     8161 ctatagtttc aagtaattta tattctccat ctatatttct taatgttcct ctaggaggag
     8221 aggcaatatt atgatattta tcaacagata taggtttaaa aatttttgtt tcagg
//
LOCUS       DAWEWP010000084         8238 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWEWP010000084 DAWEWP010000000
VERSION     DAWEWP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.084"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="WGN53_001344"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="WGN53_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ0140333.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WGN53_001345"
     CDS             complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WGN53_001345"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ0140334.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1963..2286
                     /locus_tag="WGN53_001346"
     CDS             1963..2286
                     /locus_tag="WGN53_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ0140335.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAVLLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2333..2809
                     /locus_tag="WGN53_001347"
     CDS             2333..2809
                     /locus_tag="WGN53_001347"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ0140336.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2887..3312
                     /gene="nusB"
                     /locus_tag="WGN53_001348"
     CDS             2887..3312
                     /gene="nusB"
                     /locus_tag="WGN53_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ0140337.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3680..4714)
                     /gene="pyrC"
                     /locus_tag="WGN53_001349"
     CDS             complement(3680..4714)
                     /gene="pyrC"
                     /locus_tag="WGN53_001349"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ0140338.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAVLPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQDILFLVHGEVTDPEIDIFDREAAFIERVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPVSVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4774..5379)
                     /locus_tag="WGN53_001350"
     CDS             complement(4774..5379)
                     /locus_tag="WGN53_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140339.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            complement(5380..5604)
                     /locus_tag="WGN53_001351"
     CDS             complement(5380..5604)
                     /locus_tag="WGN53_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ0140340.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            5713..6324
                     /locus_tag="WGN53_001352"
     CDS             5713..6324
                     /locus_tag="WGN53_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ0140341.1"
                     /translation="MRRTALLLLALTAGTSLAAGFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGDVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            complement(6384..7256)
                     /locus_tag="WGN53_001353"
     CDS             complement(6384..7256)
                     /locus_tag="WGN53_001353"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ0140342.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRREMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(7293..8078)
                     /locus_tag="WGN53_001354"
     CDS             complement(7293..8078)
                     /locus_tag="WGN53_001354"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ0140343.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAENA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
BASE COUNT         1988 a         2499 c         1959 g         1792 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccag accttcaaaa
      181 atactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgctcccc gcctttgccg attaaaatcg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggttcaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcta
      421 aaaaacgcgc cgatttgtcc gtaaccatat cttcgggata catcggcacg ctttcgggca
      481 gatacggctt gagcagctcc aacaggttgg caatccgcaa tccgtgtttc gcactgaccg
      541 cctccgccgc cgcaaattca aattcggcgc gcacttgggc aacaaacgcc tccagcgcgt
      601 aacggtcttt cgccttgtcc ttgtcgattt tgttgaccac taaaatgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg acacggtcgg catcggtaaa gcgcatcgct tccacgacga
      721 aaaccaccac atccacgccg ccaagcgcct cggtaacgtt ttgattcagc ctgtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaagccgg gcgtatcgac aaacacgaac tgcgcggtat
      841 cgtcggtata aatccccgtt acgcggttgc gcgtcgtctg cgcctttttg ctggtgatgc
      901 tgattttctg accgatgaga tggttcatca gcgttgattt gcccacgttc ggacgaccga
      961 caatcgccac gaagccgcaa cggtatccgc cggcggcgcg ttcccctgca aggaaggttt
     1021 caatatccat attcgctttc atatccacat ttcagacggc atattcacgc gccgcctcat
     1081 ttctttttct tcttcagcgg cagcttctct tccagccatt tcaaagcctc tttcgccgcc
     1141 tcctgttccg ccgccttgcg gctcgtccct ttggcacggc acacgaaacc cagttcgccc
     1201 aaatcgcagg aaaccacaaa catactgtcg ttggcatggc cgatttgctc ttcgatacgg
     1261 tatttcggca aagcaaagcg gcgtgcctgc aacgcctcct gcaaagcagt tttgccgtct
     1321 tttgcctgat tttgaaaatc ggcgcgccgg acgcgatcgg caaacaaatg gcgcaccacc
     1381 ttttccgccg tgttgaaatc ggcatcgaag ctgacggcag caaacatcgc ctccatcgcg
     1441 tctgccagta tcgaaggccg tctgaagccg ccgctcttca actcgcccgc ccccaagtac
     1501 aggccgtcgc cgacattcat ttccgccgcc atttccgcca gcacgccctc attgaccaga
     1561 cttgcccgca accgcgacaa ctcgccctcg gtcaacttcg gaaacgcgtc aaacagcatc
     1621 cgcgccaccg tataattcaa aatcgaatca ccgacaaact cgaaccgctc gttgtgcttc
     1681 gcatgatggc tcctgtgcgt caaagcctgc cgcaaaagcg aaatatcgcg gaacgcgtag
     1741 cccagtttct tctgtatcgc cgcgtgtgcc tgctgtttca aaacatcgtc tttcattacc
     1801 gtcttccgca aaaaacagcc cgaatgcgtt tccatactcc aaaaatacag aaacacctta
     1861 aggctgcaaa cggcgcacat tgtaccacca aaccgccgcc gtctgccgac aacgcacgaa
     1921 tcgtggtaaa atccgtaagt tttcccccaa cgtattcccg taatgaacgc atttgcacaa
     1981 gcactttcct cggcactcga ccgctgtatc gccaccaaca ccgtcgccaa acaaaaccgg
     2041 cccgtcggct tcctctaccg cgaagccccc gtttttgaaa acgacagcgg ctggcgtttc
     2101 ttcagcggcg acgagaccga cgaatacacc gacgatcccg acaacttcag catcgtcagc
     2161 ctcgccgaca tcgccaaaac caaccctgaa accgccgtac tgctttccca acctgaaggc
     2221 agcgcgtggg aattggcaga agacggcaca ttccaaaccg tcgccgactg gcaaccgcaa
     2281 gactgaaacc ttcagacggc attccgaaac cccgaatacc aaaggacacc caatgaacac
     2341 catcgccccc aaccttgacg gcaaacacct ccgcatcggc atcgtacagg cacgtttcac
     2401 caacgaaatc ggcagcgaaa tgctcaaagt ctgctgccgc accctccaag aattgggcgt
     2461 ggcagacgaa aacattaccg tcgccaccgt acccggcgcg cttgaaatcc ccatcgcgct
     2521 gatgaacctt gcctcttctg aaaaatttga cgcactgatt gccatcggcg tcgttatccg
     2581 tggcgaaacc taccatttcg agctggtttc caacgaatcc ggagcagggg tcagccgcgt
     2641 cgcactcgac tacaacatcc cgattgccaa tgccgtcctg accaccgaaa acgacgcaca
     2701 ggcaatcgaa cggattgaag aaaaagcctc ggatgccgcc aaagtcgccg tcgaatgcgc
     2761 caacctcgtc aacctgctgc tcgaagaaca gtttgaagac gaagaataac aaacagggcg
     2821 tacaccaata cgccctttcc gtttcagacg gcataccgtg ccgcccaacc gtttcaagga
     2881 aaaatcatga aaacagcccg ccgccgttcc cgcgagcttg ccgtacaagc cgtttaccaa
     2941 tcccttatca accgcaccgc cgcgcccgaa attgctaaaa acatccgcga aatgcccgac
     3001 tttgccaaag cggacgaaga attgttcaac aaacttttct tcggtacgca aaccaacgca
     3061 gcggactaca tccaaaaaat ccgccccctg ctcgaccgcg acgaaaaaga cctcaatccc
     3121 atcgaacgcg ccgtcctgct gaccgcctgc cacgagctgt ccgccatgcc cgaaacgccc
     3181 taccccgtca ttatcaacga agccatcgaa gttaccaaaa ccttcggcgg cacggacggg
     3241 cacaaattcg tcaacggcat cctcgacaaa ctcgccgccc aaatccgccc cgacgagccc
     3301 aaacgccgtt gatgcatcac aaatttaaag aaagttctat gtccttattt ctgctttctt
     3361 tgtttcgtca ttcccacgca tgtgggaatt tggttttttg aattttgggt atttcaaata
     3421 aattctgaaa cttcaatctc gtcattcccg cgcaggcggg aatccggttt tttgagttgc
     3481 tgccgttttt gataaactct agtcgcatcg aatttccaga ttcctgcctt cgtgggaatg
     3541 gcgaaatttg aaaattgtgg gaatttatcg gaaataaccg aaacgtaaca aactcaaacg
     3601 ccgttgacgt atcaatcagt cagcaacaca aatgccgtct gaaagccctc ccttcagacg
     3661 gcattttgtt tccagcccat caatactgca ccgtccagcc gatttcgccg cccgcgcgca
     3721 tcgggacaag ttcgccgtcg ccgtagggaa cacttacggg gacggtttgg ctttgtttga
     3781 cgagggtgat cgtgccggcg ttttccggaa tgccgtaaaa ccttgcgcca tttttcgagg
     3841 caaaggcttc gagtttgtcc aacgcgcctg ctttttcaaa cacttcggcg taaagctcga
     3901 cggcggtcat cgcgctgaac ataccggcgc agccgcaggc gttttctttg gcggacttgg
     3961 cgtgcggcgc ggagtcggtg ccgaggaaga atttgtgcgc cttctcgccg gtaacggcgg
     4021 caaccaatgc ctgacggtgg gtttcgcgct tgaggacggg caggcagaaa tgatgggggc
     4081 gcacgccgcc gaccaagagg tcgttgcggt tgagcaggag gtgttgcggg gtcacagagg
     4141 cggcaacgtt gtcgcctgct tccaaaacca ggcgggcggc ttcggcggtg gtaatgtgtt
     4201 caaacacgac tttaagattc ggcacttgcg ccaaaacggg tttcatcacg cgctcgataa
     4261 aggcggcttc gcggtcgaaa atgtcgattt cggggtcggt tacttcgccg tgaacgagga
     4321 acaggatgtc ctgttttgcc atttcttcca acacggggat gagcttgaat aggtcggtta
     4381 cgccggaatc ggaattggtc gtcgcgccgg cggggtagag tttgaaggcg acgatgccgg
     4441 cggctttggc ttcgcgcacc agctcgggcg tggcttggtc ggtcaggtaa agcgtcatca
     4501 gcggctcaaa cgcgctgcct tcgggcaaca ccgccataat gcgcgctttg taggcaagcg
     4561 cgtcggctac actgacgacg ggcggtttga ggttgggcat aatgacggcg cgccccattt
     4621 ggcgggcggt ataaggggca acggctttga gcgcgtcgcc gtcgcgcagg tgcaggtgca
     4681 tatcgtcggg gcggataatg gtcagggttt gcatggtttt cctttgtttt atttgaatgt
     4741 tcagacggca tgccgacttt atgccgtctg aagttagggg agaattaggg gtttcccgtt
     4801 gtgggaacgg tgcgtcaggt tcaaaactgc ctgtacgggt aaatccgggg aatcggcggt
     4861 ttgtgcggtc aatacggttt cttcggagct gcctgcttcc cgccaggcat agctgacttg
     4921 gcggatttca aatgatgcct gcaccgactg ttccgccctc aattctatat tggcattttt
     4981 cggcggctgt cggacaagtc cgtcaaaatg ttccaacagc gcggcactgt ctgcccgctc
     5041 ggctttcaca ctgcaactgt cgcgcgtcag gctcaaataa taaatgccgc catcgcgttc
     5101 aagcgtccac aacgcctgta catcgggttc tgcaacagtg ggcggcagat gggacaggtc
     5161 ttcctttccg acagggacaa aatgtccgac agcggcaaac ttggaaaccg attcaaaact
     5221 gccttgagtc aggacgcagg cactccgaaa caaatcgacg gctacacgcg cgaaatcctg
     5281 atgccgtaca ccggattgcc ggttttgggc attacacgca gcaagcacaa atgccgacag
     5341 caataacatc aatcgggaca tccgcatttc agacggcatt tatttgtgct tgacgaccag
     5401 cgtgaacacg ccgtcctgtt cggaagcatc caacagcaca tgaccggttt ggcggcaaaa
     5461 agcctcaaaa tcccccggtg cgccgccgtc ggttgccaga acggtcaaca cgtcgccctg
     5521 ctgcatttgc gccaaagcct ttttcgcccg caaaatcggc aggggacatt tcaatccggt
     5581 tacgtctaaa gtttcgctgt tcatcgtttg taccgtttaa aaaatgggaa tcggggggag
     5641 attatacccc agttgcttgc agcccccttt gccaacccat aaaataacgc cgttttcaat
     5701 ttggagaaac ttatgcgccg caccgccctc ctgctgctcg cactcaccgc cggcacgtcc
     5761 cttgctgccg gcttcaacca aaaagacacg ccgatcaaca cccgctaccg cgaaacgccc
     5821 gaagaagccg ccgcccgcga atttaaagaa cataccgcca aactgccgcc gctgcccgat
     5881 acgcactcgg acggctggtt cgacatttat gtggatgaaa attacggcaa acagccgaaa
     5941 atcctgctcg acagcctgca aattatgccc gcgcccgacg gcagcatccg ctacattctc
     6001 aatatccgct ccgacaaagg ctacgacaac ctgactgccg aaggcatatt ctgcgcccgc
     6061 ttctccatca ggttcggcaa cggcaaacct tcctcctata aagtgttcgg ctacggcgac
     6121 acggtcaacc gccgctggat acagccccga aacgccgaat ggaaaccgat aggcggtaca
     6181 ttaagccgca acgacgcact gcgcaccgtc ctgtatcagg cattctgcga gggcgatgta
     6241 cccgccgaca cgaaaggttt ggtacaacgc ctgaaagaac gtgccggacg ctatgccccg
     6301 tcgataaagc cgcacaacaa ataaccagac acaaaaatac cgtctgaaac atcggtttca
     6361 gacggtattc gattcatcag ccatcaagcg gcggaaactt tgtcctgacg gcacaacagc
     6421 gtaatcaaat cactgatgcg ccgcttcatc tcacgccggt caacaatctg gtcgattgcg
     6481 cctttctcca gtaaaaactc agcacgctgg aagccttccg gcaaggtttc ccgtacagtc
     6541 tgttcaataa cgcgcggtcc cgcaaaaccg attagggcat tgggttcagc caatacgaca
     6601 tcacccaaaa atgcgaagct ggcggatacg ccgcccatag tcggatcggt caacaccgat
     6661 ataaacggca ggcgtttttc cgtcagcaaa tgcagcgcgg cactggtttt cgtcatctgc
     6721 atcaatgagt tcacaccctc ctgcatacgc gcaccgccgg aagccgccac acagacgaac
     6781 ggacaattgt cggcaaccgc ccggcggacg ccctgtacga atcgttcgcc cacaaccgaa
     6841 cccatcgaac cgccgataaa gcggaactca aacgcagcaa ccacaacggg cagaccgttc
     6901 atcttgcctt tcattaccac caaagcatca tcttccccgg tcagcttgcg tgccgcactc
     6961 aaacgatccg gatatttttt gccgtcttta aacttcaaag gatctgtcgg tttgacattt
     7021 ccggcaacct cctctctgcc atcctcatcc aaaagcagat tcaggcgttg tcgtgccgac
     7081 aacggattgt ggtggttgca tttcggacaa acctgattgt tctgctgcaa ctcggtcgaa
     7141 taaacggttg ccgaacaaga cggacatttg tgccacaaac cctcgggaac attggaagaa
     7201 ccgtctttgc tgcggttttt gattttgggt ggcaggattt tatctaacca gctcatggac
     7261 aactcctaaa atatttagaa tgcgtgacag aatcagcgca cggcatcctt caactctttg
     7321 accaaagcac cgacggccgc agcctcgttg cccgcattgt tttcaatctc tttcacaatc
     7381 cggctgccga caatgaccgc gtcggcaacc cggccgattt tgcgcgcgtt ttccgcattg
     7441 ctgatgccga aaccgacacc gatgggaata tcgatatact gatgcaaata ctctatttta
     7501 cgcgaaacct catccgtatc caaacttgcc gcgcccgtta cgcccttgag cgaaacataa
     7561 tagacaaatc cgcctgccag ctcggcaatg gttttaatac ggtcttccgt cgtcgtcggc
     7621 gcaatcagga aaatacagtc gaccccgtta tccttcagct cgcgatagag cggatcgatg
     7681 gtttcgacag gggaatccac cgtcaacacg ccgtccacac ccgccattgc ggcttcctga
     7741 gcaaactccc gataacccat cttatgtaca gggttcaaat atcccatcaa aacaaccggc
     7801 gtttgcgtgt cggtttcacg gaattttctg acgacatcca agacatcgcg cagcgaaatc
     7861 ccgtttgcca acgcccgctc cgccgcacgc tgaataaccg gtccatccgc catcggatcg
     7921 gaaaacggca cgcccaactc caaaatatcc gcaccgtttg caaccatgcc gtgcatcaat
     7981 gccaaagttg tccgaatatc ggggtcgccc accgtaatat agggaatcaa tgcctttccg
     8041 ccatccaaag cggcaaaagc ctgccggatt ctgctcattt gtctcttcca tcctatcggc
     8101 aaatatgcca taaaacacaa tcagtaaatt tttcccagta taacatttgc ggtacggata
     8161 ggcgaatacg gcaaacagaa accacgcaaa tcagagccgc cgtttgaacg aggcggaaat
     8221 aaacggcaag catctcgg
//
LOCUS       DAWEWP010000085         8220 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWEWP010000085 DAWEWP010000000
VERSION     DAWEWP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.085"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(63..623)
                     /locus_tag="WGN53_001355"
     CDS             complement(63..623)
                     /locus_tag="WGN53_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ0140344.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WGN53_001356"
     CDS             complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WGN53_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ0140345.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2243..3284
                     /locus_tag="WGN53_001357"
                     /pseudo
     CDS             2243..3284
                     /locus_tag="WGN53_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3542..4180)
                     /locus_tag="WGN53_001358"
     CDS             complement(3542..4180)
                     /locus_tag="WGN53_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ0140346.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4361..6196)
                     /gene="edd"
                     /locus_tag="WGN53_001359"
     CDS             complement(4361..6196)
                     /gene="edd"
                     /locus_tag="WGN53_001359"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ0140347.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYASMPKSIKIEMLQDTVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKAGNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLSAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLIDEAE
                     WNAREVEHIDLGANQQGCGRELFAGFRSLTGGAETGAMSFGGEFA"
     gene            6712..8157
                     /gene="zwf"
                     /locus_tag="WGN53_001360"
     CDS             6712..8157
                     /gene="zwf"
                     /locus_tag="WGN53_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ0140348.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAARGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT         1922 a         1951 c         2269 g         2078 t
ORIGIN      
        1 ctaaatatct agcaaaaaag tgcggtcaaa attcaccgca cttttcgttt cgaacaatcg
       61 aattatttaa tcacgccaca tgccatacgt gggccgccac cgccaagtgg agctggatga
      121 tcggagtgat tatcaccacc cgtgtggatc ataatagagt gaccgcgaac atcatctaaa
      181 tgtttaagac gtggtgctaa aacaggattt gttgctgtgc catcatgcaa tacagttaat
      241 gcaggtaaat cacctaagtg tgcatcatct tgccatgggt aaccatgttg ttttgcacct
      301 ttaggatccc agtgaccgcc tgcgcctaaa ccagctgtca atttaccttc tttttctttt
      361 ggctcacagc ttgggttttc atggatgtgg aaaccatgta agccttcgct taatccttgt
      421 aaatcagggg taaacacaag accatagtta gattcagtaa tagtcactgt acccacatct
      481 ttgttaccgt ttactggatc aagttgttgc actttcactt caatagaagc acctttaggt
      541 atcgtattat gctcgtgtgc ttgcgcaaca ccaactgaac atactgcact aaccgctagt
      601 gctaataagg ttttcatatt cattgcaatc tcctttattg cagtaaaaac tcgcgactat
      661 tttagtcaat tttgttttaa aagccgataa atttttacga aaattttaca ttaattgtta
      721 tttgcagaaa attagaaccc gttatcacgt cttggataaa tgccaaaacc attgtgattc
      781 agagaattaa aaagaaaaat ttcggaatcc tcatcctttt ctgaaacctg attgcggtat
      841 ccatggcttt gtctttgtca tttaagtttg tgttcatgtt tttgaccttc tttgcttttt
      901 aaatcctttt taaattgccg acttcttccg ttccaaatgt cccatttgaa cattatcccg
      961 ccgcctaaaa tacgacccaa attcatcggt tgttcttgct gcgatacctg caaacaacag
     1021 ttcaatgggt ttattttgtt tattctaacc tattaccgtt taaataaatt tccaaaggct
     1081 tgggcaggcc gtaatctttc aaatgcgtcg gctttatcca aatgccgtct gaagggcttt
     1141 cagacggcat ttgtccttca aacggcgtaa tcatcagcaa ccggtgcgtc agccggtggg
     1201 tcagggcggt ttgttcgtcc atatctgcca tggtcaggga gaatttggcg gcaaagtcgg
     1261 aaagcccgtt caaactttca aaacacggca cgcaatacag cccgccccaa atgcctttgg
     1321 cggggcgttt ttccagcaaa atcgcgccgt tccggttgcg gataatcagc cagtaaagcg
     1381 gcagggtcgg tacttcggcg gcggtttttt tgcgcggcag ctcggcggtg cggttttgct
     1441 tttttgcttc gcagatgtcc gccatcgggc attggtggca caagggtttc gtccgtttgc
     1501 acacggttgc gcccaaatcc atcaaacctt gtgtataggc aggcatatcg gcgttttcag
     1561 acggcagcag gctttcggca agtgtccaga gcgagttttc aaattttttg tcctgcggat
     1621 tgccgtcgcg ggcgaacacg cggcagagta cgcgtttgac gttgccgtcc aaaatggttt
     1681 cgcggcggtt gaaggagaag gcgcaaatgg cggcggcggt gcttctgcct acgccgcaga
     1741 gcgtttccaa gtctttgcgt tccgatggaa acgtgccgcc gaattgcctg acgacttgtt
     1801 gcgcggcttt gtgcaggttg cgcgcgcggc tgtaatagcc caagcccgcc cacaacgaca
     1861 acacttcgtc ttgcggcgcg gcggcaagcg tctgaacggt cgggaatttt tctaagaaac
     1921 gcggatagta gtccaacacg gtggcgacct gcgtttgctg gagcatgatt tcggaaagcc
     1981 agacgcaata agggtttttg acctgccaag ggaggtggtg gcgaccgtgt tgtttttgcc
     2041 agcggatgag ccgttcggag aaggggatgg gtgtgttcat taatatcaat cggtggtttt
     2101 atttatattt aaaacagtat gttattgtat aacattgtga aaataattct tattgactta
     2161 ttttttgtag gggtatataa ctcatataaa gaaactttat tgcggtattg aaattattta
     2221 tcaacaagca aggagtatca gcatgaaagc aatggtttat cacggcgcaa acgacatccg
     2281 ttttgaagaa aaaccccgtc cgcagattat cgatccgacc gatgcggtgg tgaaaatcgt
     2341 caaaaccacg atttgcggta ccgatttggg tatttggaaa ggcaaaaaaa accgaagtcg
     2401 ccgacggtcg tattctcggt catgaaggca tcggtattgt agaagaagtc ggcgaggctg
     2461 taaaaaacat caaagtcggc gataaagtca ttatttcatg cgtcagcaaa tgctgtactt
     2521 gcgacaactg caaaatccaa ctttattcgc attgctgcaa cggcggctgg attttgggct
     2581 acatgattga cggcacgcag gcagaatacg tccgcacgcc ttatgccgac aacagcctcg
     2641 tgccgctgcc cgacaacgtc aacgaagaag tcgccctgtt gttgagcgac gctttgccga
     2701 ccgcacacga aatcggcgtg caatacggcg atgtcaaacc cggcgacacc gtattcatcg
     2761 caggtgccgc tcctgtcggt atgtccgcct tgttgaccgc ccaactgtat agccccgccg
     2821 ccattatcgt gtgcgatatg gacgaaaacc gtttgaaact ggcgaaagag ttgggcgcga
     2881 cccataccat cagccccgcc tcaggcgatg tctccaaaca agtttttgcc attgtcggcg
     2941 aagacggcgt agattgtgcc atcgaagccg tcggtattcc tgcgacttgg aatatgtgcc
     3001 aagacatcgt gaaaccgggc ggtcatatcg ccgttgtcgg cgtacacggc caatccgttg
     3061 atttcaaact ggaaaaactc tggattaaaa acctcgccat cactaccggt ttggtcaatg
     3121 ccaatactac cgaaatgctg atgaaggcaa tttccagcag ctcagtcgat tacaccaaaa
     3181 tgatgaccca ccatttcaaa ttcagcgaat tggaaaaagc ctacgacgtg ttcaaacacg
     3241 ccgccgaaaa ccaagcgatg aaagtggttt tggaagcgga ttaatcggcc ttgaatcagt
     3301 tgttttttta agcggatgcc gtctgaatgc gtttcagacg gcattttttc agccgcgtag
     3361 tatgggctgt ccggctgctg tcgaatgaac tgaacgttat ttttattatg cggaaggcgg
     3421 atgggcattt taggggcggt atgtctgatc agtgctgccg tgtatgccga ctgtgccctc
     3481 aatcggcaaa atgccgtctg aaacgggaaa ggtttttcag acggcatttt tacgatgcga
     3541 atcaggcttt gggtttcaac gccgccgctt ctttggcgag gcgggtgatg gtgtcccagt
     3601 ctttgttttt cacggcttct ttcggtgtca gccaagagcc gccgacgcac aggacgttgg
     3661 gcagtgccaa gtactcgggc gcggtggcga ggctgatgcc gcctgtcggg cagaagcgaa
     3721 catcggcgta aggaccgtac agggctttga gcatggcttt gccgccaacg acttcggcag
     3781 ggaagagttt gagggtgtcg atgccgtgtt ctaaagccag ttggacttcg cccggggtgg
     3841 caacaccggg aatcaggggg atgccgctgt tgtggccggc tttggcgagg gattcgtgca
     3901 gccccgggct gatggcgaaa accgcgcctg cgtcttcgac ggctttgagc tgttcgggat
     3961 tggttaccgt acccgcgccg acgatggcgt tgggcacttc tttggcaatc aggcggatgg
     4021 cgtcgaggcc gacaggggtg cgcagggtga tttcgagggt agggatgccg ccttcgacaa
     4081 gggcgtggga caaatcgatg gcggtgctta agtcgtcaat cgccattacc ggcacaactg
     4141 cgccggcggt caaaatttcg cggggggtca gtttggacat ttcggttctc cgggtggaat
     4201 ggggtatttt attaagatgg gacaggttgt aggtcggatt caagaaaccg acatcttgga
     4261 cggctgtgtc ggattcgaga atccgaccta cgtctatctt aaaacgcttg aataattttt
     4321 cagacggcct tcggtctgaa aaggccgtct gaaacgcaca tcaggcaaat tcgccgccga
     4381 agctcatcgc gccggtttcc gcgccgccgg tcaaactgcg gaagccggcg aagagttcgc
     4441 gaccgcagcc ttgttggttc gcgcccaagt cgatgtgttc gacttcgcgg gcgttccatt
     4501 cggcttcgtc aatcaggacg ttgagttcgc cgctaacgga gtcgaagcgg atcaggtcgc
     4561 cggtacggat tttggcgatg ttgccgccca tcagggcttc gggtgtcatg tggatggagg
     4621 ctggaacttt gccggacgcg ccggacatac gaccgtcggt cagcagtgcc actttgaagc
     4681 cgcggtcttg caggatgccc aaaggcgggg tcagtttgtg caattcgggc ataccgttgg
     4741 cacgcgggcc ttggtagcgg acgacgcaga cgaaatcgcg ttccaactcg ccgcgttcaa
     4801 atgcggacaa tacttcgcgt tggtcgttga acacgatggc aggtgcttcg atgatgcggc
     4861 agccttcgcg cacggcggac actttaatca cgccgcgacc gatgttgcct ttcatcaggc
     4921 gcagaccgcc gtcgggggag aacgggttgc cggctttgcg caggatgtcg tcgttgccgc
     4981 tggtttcggg ggcttcgcgc cattcgagtt tgccgtcgat aaggaaaggc tctttggtgt
     5041 agtggcgcat accgtgtccg acaacggtat cgacatcgtc gtgcaacagg cctgcgtcca
     5101 gcaattcgcg gataacgaaa ggtagaccgc cggcagcggt aaagtggttt acgtcggctt
     5161 tgccgttggg gtacacacgg atcagcaatg ggatgatgga agaaatttcg tcaaagtcgt
     5221 cccagttcaa aatcacgccg gcggcgcgtg ccatggcaac gaggtgcatg gtgtggttgg
     5281 ttgagccgcc ggttgccatc aggccgatta gggcgttaat gaaggatttc tcggtcaaca
     5341 tttcgcccaa tggtttaatc gtgccgtttt tgatgccgcg tgcgaggtgt ccggcggcat
     5401 agcgggtcag ggcttcacgc aggtcggtgt aagggtggac gaaggcggcg gcaggcaggt
     5461 gcacgcccat catttccatc atcatttggt tggagtttgc cgtgccgtag aaagtgcagg
     5521 tgcccgggct gtggtaagaa cccatttcgc ttttcaaaag ttcgttgcgt ccgaccttgc
     5581 cttcggcgaa aagctggcgg gtgcgggctt tttctttgtt gccgataccg ctggacatcg
     5641 ggcctgcggg gacgaagata cccggaatat ggccgaaaga caacgcaccg atcatcaaac
     5701 cgggaacgat tttgtcgcac acgcccataa acaggctgcc gtcaaacatt tgatgagaca
     5761 gaccgacagc agtactcatg gcaattacgt cgcgggagaa cagcgacaat tccatgccgg
     5821 catagccttg ggtaataccg tcacacatgg cgggcgtacc gccggcaact tgcgcggttg
     5881 cgccgttttt ctgcgcttcg tctttgattt ggtcagggaa gtctttaaac ggctggtgtg
     5941 cggaaaccat gtcgttgtag gcggtgatga tgcctaagtt gggaacggtg tcctgaagca
     6001 tttcgatttt gatgcttttg ggcatggagg cgtagccgtg cgccaaattg ctgcagccga
     6061 gttggttgcg ctctaagcgt cccatctgtt tggcactacg gattttcgcc agatattttt
     6121 cacgcgtcgg acggctgcgt tcaataatgc gcccggtgat ttcggcgagt ttggagtgga
     6181 taggagtagt gttcacggtc ggtctcctgt cggaacgggg ggtattgggg atttgtttgt
     6241 gcgtttctga tttttgtatc gggtattcag acggtgagta tagtggatta acaaaaacca
     6301 gtacggcgtt gcctcgcctt gccgtattat ctgtactgtc tgcggcttcg tcgccttgtc
     6361 ctgatttttg ttaatccact atattttaaa aggtataccg tctgaaagtg ggaagcgggt
     6421 gttattatag tacaaaattt gtaattttgt tacaaaaaaa tcagacatcg aaaagaaatt
     6481 tgatttaaat cgcagaatct tccgattgtt gtaacggaat aggggaaacc tgcccgattt
     6541 ttggactagg tcaaataaat tgtaaaaatg ggaatagaca aaaagtgtag cggtgtgtag
     6601 tattactact cattatgccg cctgcctttg tttccgatat tgcgatgcag cgtgatgggg
     6661 gaaacaggtt tcagacggca tcgaaaaatt ctttcctgac gagataatac gatgagtaca
     6721 caaacaaatt ttgatttggt gttgttcggt gcgaccggcg atttggcgat gcgcaagctg
     6781 ttgccctgtc tgtaccaggc gcatgttgcc ggtctgcttc atcccgaagg gcgtatttta
     6841 ggggtcagcc gcagcgagtt ggataccgaa ggttttctgg cgaaggtgga aaccagttcc
     6901 aaaatccatg tgaaagaaaa tttttccgat gaggcttggg catcgtttgt cgaacgtctc
     6961 gcatatctca aagtcgatgt tacgcagccg gacgattttg ccgctttggg cgatttggtc
     7021 aaagcgcgca aagaaaccga caatgtcgtc atctatcttt ccaccgcgcc gaaattcttc
     7081 gcgcaaacat gtgaaaacct tgccaaaatc ggtttgaacg ccgacaacgt gcgtgtggtt
     7141 ttggaaaaac cgttgggtac ggatttggct tcttcccagc aaatcaatac cgatgttgcg
     7201 cgttatttca aagaagggca aatttaccgc atcgaccact accttggcaa agaaagcctg
     7261 caaaacctgc ttgcgctgcg ttttgccaat gtaatgttcg agccgctgtg gaacaataaa
     7321 tacatcgaaa gcgtgcagtt gaccatcgcc gagcagttgg gcgtggaaga gcgcggcgag
     7381 ttttacgaca ttaccggcgc gttgcgcgat atggtgcaaa accatttgat gcagatgctg
     7441 tgcatgactg cgatggaagc ccccgccagc ttggatgccg acgcggtgcg cgatgaaaaa
     7501 gtcaaagtca tcaagtcatt gaagccgctg accgtcgagt ctgtcaatga aaacgtcgtg
     7561 cgcggacaat ataccgccgc cagaggcatg aacggctatc ttgaagaaat caacgttccg
     7621 caagacagct ttaccgaaac ctacgtcgcc attaaagccg aaatcgaaaa cgaacgctgg
     7681 aagggcgttc ccttctacct gcgtaccggc aaacgcatgg cgggcaaagt ggcggaaatc
     7741 gttttgaact tcaaagattt gaacagccat atttttgaag gcagccgcac cgcgcccaac
     7801 cggctcgtta tcgagttgca accatatgaa tccgtgcgcc tctatacgca gatgaaaacc
     7861 ccgggggcag gaaataaggt cgaaaccgtg ccgctggcaa ccgatttggg caaagcattg
     7921 gaaagccgcc gcgcggaagc ttacgagcgc ctgctgctgg atgtgattaa cggcaaactc
     7981 gctttgttta accgccgcga cgaactcgaa gccgcgtggg aatatgtgat gccgattttg
     8041 gaaaactgga caaataacac cacgccgccg cacggctacg gcgcacactc gtgggggcct
     8101 gaagccgcgc gcgaactatt ggcgcgcgac ggacacaagt ggcacgaaga gcagtaatac
     8161 aataatgcgt tcagacggca tggggtttga aatgccgtct gaacgtaagt aaagcagtat
//
LOCUS       DAWEWP010000086         8135 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWEWP010000086 DAWEWP010000000
VERSION     DAWEWP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.086"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(68..292)
                     /locus_tag="WGN53_001361"
     CDS             complement(68..292)
                     /locus_tag="WGN53_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ0140349.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(293..1681)
                     /locus_tag="WGN53_001362"
     CDS             complement(293..1681)
                     /locus_tag="WGN53_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ0140350.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1767..2588)
                     /gene="prmC"
                     /locus_tag="WGN53_001363"
     CDS             complement(1767..2588)
                     /gene="prmC"
                     /locus_tag="WGN53_001363"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0140351.1"
                     /translation="MTFDEWLGLSKLPKNEARMLLQYASEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYLLGWREFYGRRFAVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPSLETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2595..3038)
                     /locus_tag="WGN53_001364"
                     /pseudo
     CDS             complement(2595..3038)
                     /locus_tag="WGN53_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGN53_001365"
     CDS             complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGN53_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ0140352.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      4765..4868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4935..6158
                     /gene="cytX"
                     /locus_tag="WGN53_001366"
     CDS             4935..6158
                     /gene="cytX"
                     /locus_tag="WGN53_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ0140353.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGVSANNISAKLSETPIAVAVAVVGTLLAVLLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            6155..7255
                     /locus_tag="WGN53_001367"
     CDS             6155..7255
                     /locus_tag="WGN53_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0140354.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECTPEDLQAQYDWLIDCRGYGAKTAWNASAEHGSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVVGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSLARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALVDGKDAPERDEESGLAYIRRQD"
     gene            7276..7893
                     /gene="thiE"
                     /locus_tag="WGN53_001368"
     CDS             7276..7893
                     /gene="thiE"
                     /locus_tag="WGN53_001368"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ0140355.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAENPEAVVKAFQALWDG"
BASE COUNT         1736 a         2505 c         2204 g         1690 t
ORIGIN      
        1 cttaggtttg catcggcgga atattcaaac acagcctttt ttaaggaaat ccggatacgg
       61 cggcgcatca ataatgcggc ggaatctcgt cgcgcaggga atacggctct tgcgcgtcgg
      121 gattcctgtc ctgcattttt tgatacagca gcctcaactg agcctgctgc aaatccagcg
      181 tctgccgcaa ttccgccacc atcgcgttca ggccggcgat tacgtcctcc tgaagcgcgg
      241 attggatttc cagctctgtg atgcggtgtt ccaactcttg aaccgcgtcc atttacagca
      301 ccatcgcggc aatccagccg gcaatcagca gcgggatatt gtagtggatg aaggtcggga
      361 taacggaatc gcggatgtgg tcgtgttgtc cgtcggcgtt cagccccatc gtcgggccca
      421 acgtggaatc ggacgcaggc gaaccggcat cgcccaacgc ccccgccgtg ccgacgatgg
      481 cgacggtggc aagcggcgaa aaacccaaac cgacacacaa aggcacataa atcgcggcaa
      541 taatcggcaa agtggaaaag gacgaaccga tgcccatcgt taccaaaagc cccaccacca
      601 gcatcgccaa tgccgccata cctttgctgt tgccgaatat cgccatactg ctttccacca
      661 gcggctgaat atgcccggtc gcattcatca cggcggcaaa accctgcgcg gcaatcataa
      721 tgaagccaac catcgccatc atcttgatgc cttcgccgaa tacgtcgttt gccttgtcgc
      781 ggttgatgac gcccaacatc ataaatacgg cgaaaccgag catcgcgccc aacaccagcg
      841 agtcttcata catcaactgg atggcaaagc atacggcaat ggcggcggcg gcggccaggc
      901 tgcggtaggc ggacggctgc ggacggtttg ccgcatcggc gttgcccgcc gtatcggcat
      961 tgttgctttg gtacaggcgc ggtttgcggt aatggacaaa cgccagcagg agtccggcca
     1021 gcattcccaa cgcgggaatc gccattgccg ccattacgtt gatgtttttc acatcaagct
     1081 gcggcgcggc ggaatggatg ttgcccaaca ggatttcgtt caaaaaaatc gcgccgaagc
     1141 cgtaaggcag gaacatatag gtcgtaacca gcccgaaagt aatgacgcac gcaatcaggc
     1201 ggcggtcgat tttcaggcgg ttgaacacca aaagcagagg cggaacaatc atcgggataa
     1261 aggcaatgtg gatggggatg atgttctgac tcatcatgcc catcacaagg atgatggaaa
     1321 gcagcagcca tttgaccgcg ccctcgcccg aacgcacgct gtcgggcata ccgccccggt
     1381 tcagcttgcg gacgaccgcg ccggcaagct gctgcggcag gccggaatgg gtaatcgcca
     1441 ttgcaaacgc gccgagcatc gcataagaaa gcgcaatctt cgcaccgcct tccaaacctt
     1501 tgttgaacac ggggataatc cccgcctggc tgacctgtcc cgccgcatcg gcaatgtttt
     1561 gcagcggcat acccgccacc gcgccgccga caaacgcgcc gaccgtcagg ctcaatacca
     1621 cgtgcacgcg cgacagcgac agcaccagca taacgattac ggcaactacg actgcattca
     1681 ttgtttatcg ctccaaacct ataaatgttt acatatcgaa acacatcata acccaataac
     1741 ggaaaacccg ccaattttgc aaacaattat ttcaaatgct tcatatactt ccccagcgta
     1801 accctgtcca aacccgccaa gtccggcagg gtttccactc ccgaaaaacc attctccgcc
     1861 aacacgccgc gcaccgccgc gccctgatcg aaaccgtgtt ccagcaataa aaaaccgcct
     1921 tccgccaaac ggtcgggcgc gccttgcgcc aaggtgcgga tgcagcttag gccgtctgaa
     1981 aagtcggtca gcgcgatttg cggctcaaac cgcaaatcgc cttgcgacaa atgtttatca
     2041 ccgttttcga tatagggcgg gttggacacg atgatgtccc atttcccttc agacggcata
     2101 tcggtgtcga accacgaacc gtgtgcaaat tcgacccgcg cgcccaaatc cgccgcattt
     2161 ttccgcgccg tttcaaggga gggcgggctg atgtcggatg cgcgcacaaa cgcatcgggg
     2221 cgttcgagcg cgacggttac ggcaaccgcg ccgctgcccg tccccaaatc ccacacgcgc
     2281 ccgttttccg gcaggcgcgc caatacggct tcgaccaaat gttcggtttc ggggcgcgga
     2341 atcagcacat tcgggttgac ggcgaaacgc cgtccgtaaa actcgcgcca gcccaacaga
     2401 taggcaaccg gctcgccgtt cagacggcgt tgcgccagcc tgtccgcccg ctgtcggact
     2461 tcgtccggca tttcttcacc gccccgcgtc aacaactgca cgcgcgtata ttccgaagca
     2521 tattgcagca gcattcttgc ttcatttttg ggcagttttg acaagcccaa ccattcgtca
     2581 aacgtcattt ttatcccgtc tgccgctgat gcggcttttc tttccttatt ctttccggca
     2641 aacgtaccga tggtggcaac cgcaaatgcg gcataccaca aataaaatcc tgcaccgtag
     2701 cgcacaatat ccgatgtatt ccctgcttca tcgacgtata cggctttcac actgaaagcc
     2761 accaacgcca agccccaaag tgccgcatgg acaggcacga ccttcttccg caacgccagc
     2821 aaaacaatgg ccgccaacca aacataattc gcatagaccg cacaatacct gatatccaaa
     2881 gaagcaaata tcgaccccaa aatcaaaacg gtcaaaccct ccatgcttcc atgattgccc
     2941 aaataaaatg caacattgga taaagacgct atccacaggg caaccgacac cagcaacatc
     3001 actatgggaa aacttgattt ccgattctgt tcctgcatgg ttttatccta atgtaaaagg
     3061 ccgcctgaaa acctttcaga cggcatcgtg ccggattccg cgtcagattg cgctgccgcc
     3121 gacggtcagc ccggcatcga tacgcagggt cggctgcccc acgccgacgg ggacgctctg
     3181 cccttctttg ccgcacacgc cgacaccgct gtccaacgcg gtatcgttgc ctatcatcga
     3241 aacgtgtttc agcacttcgg gcccgttgcc gataatggtc gcgcctttga cggggtattg
     3301 cagcctgccg ccttccaccc accatgcttc ggacgcgctg aacacaaatt tgccgctggt
     3361 aatgtccacc tgtccgccgc caaagttgac ggcgtaaatg cctttgtcga tggacgcgat
     3421 gatttcttcc ggctcatagc tgccattttc cataaaggta ttggtcatgc gcggcatcgg
     3481 gacggaggcg tagctttcgc ggcggccgtt gccggtggat tgcgtacccg tcaggcgggc
     3541 attggtttcg tcctgcatat agccgactaa aatgccgtct tcaatcaata cggtgcggcg
     3601 ggtttcgttg ccttcgtcgt cgatgttgag cgaaccgcgc cggccggcaa tatcgccctg
     3661 atcgacgacg gtaacgcctt tggcggcgac gcgctcgcct attctgccgg aaaagacgct
     3721 ggtccccttg cggttgaaat cgccttccaa accgtgtccg accgcttcgt gcagcaacac
     3781 gcccggccag ccgttgccca aaacgacggt catttcgccg gcgggcgcgg ggcgggattc
     3841 gaggttggtg agtgcctgtt tgacggcggc atcgacaaac tgccgcacca agttttcatc
     3901 gaaataagcc aagtcgtagc gtccgccgcc gcccgcgctg ccctgttcgc ggcgttcgcc
     3961 ctgtttggcg atgacggtaa cgttcaggcg caccatcggg cggatgtcgg cggcgtgttt
     4021 gccgtccaga cgggcgaggt aaaccatatc gtattcgcag gtcaaaccgg ccatcacttg
     4081 cacgatgcgc ggatcggcgg ctttggcgat tgcttccact ttgttcaaca gcgcgacttt
     4141 ggcggcggaa tcgaggccgg caatggggtc ggacgcggaa caaaccggct tgccgcgcgt
     4201 ttcagacggc attttggcgg acaccttgcc gcctgccgcc ccaatcgcgc ggacggcgcg
     4261 ggcggaacgg tttatcgaat cgatgcacag gctgtcggcg taggcaaagg cggttttgtc
     4321 gcccgaaacg gcgcgcacgc ccacgccctg attgatttgg aagctgcccg atttgacgat
     4381 gccctcttcc aaatgccagc tttcataagc ggtgcgctgg cagtagatgt cggcgtaatc
     4441 gacgtggtgc gcgccgatga tgcacaggct tttggcgagc agttcggggg aaaggcggtt
     4501 ggcttcgagc agccgcgcct gtacggcgga ataggtcgga tgcatagtgt cggcgcataa
     4561 aaaatcaggg gcttgattat acggcatttg ttatatagtg gattaacaaa aaacagtacg
     4621 gtgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     4681 ttttgttaat ccactataga aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg
     4741 ccccctgctt ttgtgtacac ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag
     4801 attgcgaaag cagaatccgt agaacctgtc ggggtaatgc ctgcgtagga aacaaaccgt
     4861 caaatgcctt atcaggcttc cgttcccttt tccgcacttc cccgcccacc ttccttcttt
     4921 ttaaaggacg tcatatgtcg ggcaatgcct cctctccttc atcttccgcc gccatcgggc
     4981 tgatttggtt cggcgcggcg gtctcgattg ccgaaatcag cacgggtacg ctgcttgcgc
     5041 ctttgggctg gcaacgcggt ctggcggctc tacttttggg tcatgccgtc ggcggcgcgc
     5101 tgttttttgc ggcggcgtat atcggcgcac tgaccggacg cagctcgatg gaaagcgtgc
     5161 gcctgtcgtt cggcaaacgc ggttcagtgc tgttttccgt ggcgaatatg ctgcaactgg
     5221 ccggctggac agcggtgatg atttacgccg gcgcaacggt cagctccgct ttgggcaaag
     5281 tgttgtggga cggcgaatct tttgtctggt gggcattggc aaacggcgcg ctgattgtgc
     5341 tgtggctggt tttcggcgca cgcaaaacag gcgggctgaa aaccgtttcg atgctgctga
     5401 tgctgttggc ggttctgtgg ctgagtgccg aagtcttttc cacggcaggc agcaccgccg
     5461 cacaggtttc agacggcatg agtttcggaa cggcagtcga attgtccgcc gtcatgccgc
     5521 tttcctggct gccgctggcc gccgactaca cacgccaagc acgccgcccg tttgcggcaa
     5581 ccctgacggc aacgctcgcc tatacgctga cgggctgctg gatgtatgcc ttgggtttgg
     5641 cagcggcgtt gttcaccgga gaaaccgacg tggcaaaaat cctgctgggc gcaggtttgg
     5701 gtgcggcagg cattttggcg gtcgtcctgt cgaccgttac caccactttt ctcgatgcct
     5761 actccgccgg cgtaagtgcc aacaatattt ccgccaaact ttcggaaaca cccatcgccg
     5821 ttgccgtcgc cgttgtcggc acactgcttg ccgtcctcct gcccgttacc gaatatgaaa
     5881 acttcctgct gcttatcggc tcggtatttg cgccgatggc ggcggttttg attgccgact
     5941 ttttcgtctt gaaacggcgt gaggagattg aaggctttga ctttgccgga ctggttctgt
     6001 ggctggcggg cttcatcctc taccgcttcc tgctctcgtc cggctgggaa agcagcatcg
     6061 gtctgaccgc ccccgtaatg tctgccgttg ccattgccac cgtatcggta cgccttttct
     6121 ttaaaaaaac ccaatcttta caaaggaacc cgtcatgacc cgtatcgcca tcctcggcgg
     6181 cggcctctcg ggaaggctga ccgcactgca gcttgcagaa caaggttatc agattgcact
     6241 tttcgataaa ggctgccgcc ggggcgaaca cgccgccgcc tatgttgccg ccgccatgct
     6301 cgcgcctgcg gcggaagcgg tcgaagccac gcccgaagtg gtcaggctgg gcaggcagag
     6361 catcccgctt tggcgcggca tccgatgccg tctgaacacg cacacgatga tgcaggaaaa
     6421 cggcagcctg attgtgtggc acggacagga caagccatta tccagcgagt tcgtccgcca
     6481 tctcaaacgc ggcggcgtag cggatgacga aatcgtccgt tggcgcgccg acgacatcgc
     6541 cgaacgcgaa ccgcaactcg gcggacgttt ttcagacggc atctacctgc cgaccgaagg
     6601 ccagctcgac gggcggcaaa tattgtctgc acttgccgac gctttggacg aactgaacgt
     6661 cccttgccat tgggaacacg aatgtacccc cgaagacctg caagcccaat acgactggct
     6721 gatcgactgc cgcggctacg gcgcaaaaac cgcgtggaat gcttctgccg aacacggcag
     6781 caccctgcgc ggcatacgcg gcgaagtggc gcgggtttac acacccgaaa tcacgctcaa
     6841 ccgccccgtg cgtctgctcc atccgcgtta tccgctctac atcgccccga aagaaaacca
     6901 cgtcttcgtc gtcggcgcga cccaaatcga aagcgaaagc caagcccctg ccagcgtgcg
     6961 ttccgggctg gaactcttat ccgcactcta tgccgtccat cctgccttcg gcgaagccga
     7021 catcctcgaa atcgctaccg gcctgcgccc cacgctcaac caccacaacc ccgaaatccg
     7081 ttacagcctc gcccgacgcc tgattgaaat caacggcctt ttccgccacg gcttcatgat
     7141 ttcccccgcc gtaaccgccg ccgccgtcag attggcagtg gcactggttg acggaaaaga
     7201 cgcgcccgaa cgtgatgaag aaagcggttt ggcgtatatc cgaagacaag attaaagccg
     7261 tctgaaagga accccatgac cttcccgccc ttgaaatccc cgctcaaatt ctacgccgtc
     7321 gtccccaccg ccgattgggt ggggcgcatg gtcaaagcag gtgccgacac ggtgcaactg
     7381 cgctgcaaga ccctgcacgg cgatgaattg aaacgcgaaa tcgcccgctg cgtcgccgcc
     7441 tgtcaaggca gccgtacgca gcttttcatc aacgaccact ggcgcgaggc aatcgaagca
     7501 ggcgcgtacg gcgtgcatct cggacaagaa gacatggaca ccgccgacct tgccgccatc
     7561 gccgccgccg gtttgcgctt gggtttgagt acgcactccg ttgccgaact cgaccgcgcc
     7621 ctgtccgtac accctagcta cgtcgccagc ggcgcgattt tcccgaccac gaccaagcaa
     7681 atgcccaccg ccccgcaagg cttggacaaa ctgtgcgagt atgtgaaaca ggcaggcggc
     7741 acgcccgtcg tcgccatcgg cggtatcgac ttgaacaacg cccgagccgt actcgccacc
     7801 ggcgtttcct cactcgccgc cgtccgcgcc gtgaccgaag cggaaaatcc cgaagcggtg
     7861 gttaaagcgt ttcaggcttt gtgggatgga taaaaccgaa agaagaaaat tcaatttgcc
     7921 gtgtcggcaa aacttagccc gttatcgcaa acatacttaa ctttaaatgt ggcatatcat
     7981 caaattccgt catttccgcg caagcgggaa tccgccttaa aacttgagaa accatcattt
     8041 gaaaaacagt ttccgaattt caaaaatgga ttcccgcctg cgcgggaatg acggcaaccg
     8101 gccggttgcg tatcaaaaaa taaagtaatt cggct
//
LOCUS       DAWEWP010000087         8074 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWEWP010000087 DAWEWP010000000
VERSION     DAWEWP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.087"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            96..404
                     /locus_tag="WGN53_001369"
     CDS             96..404
                     /locus_tag="WGN53_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140356.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            486..1517
                     /gene="ruvB"
                     /locus_tag="WGN53_001370"
     CDS             486..1517
                     /gene="ruvB"
                     /locus_tag="WGN53_001370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ0140357.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1540..2268)
                     /gene="rpe"
                     /locus_tag="WGN53_001371"
     CDS             complement(1540..2268)
                     /gene="rpe"
                     /locus_tag="WGN53_001371"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ0140358.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            2414..2848
                     /locus_tag="WGN53_001372"
     CDS             2414..2848
                     /locus_tag="WGN53_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ0140359.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(2861..3724)
                     /locus_tag="WGN53_001373"
                     /pseudo
     CDS             complement(2861..3724)
                     /locus_tag="WGN53_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(3732..4349)
                     /locus_tag="WGN53_001374"
     CDS             complement(3732..4349)
                     /locus_tag="WGN53_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ0140360.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(4512..5127)
                     /locus_tag="WGN53_001375"
                     /pseudo
     CDS             complement(4512..5127)
                     /locus_tag="WGN53_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
     gene            5333..7105
                     /locus_tag="WGN53_001376"
     CDS             5333..7105
                     /locus_tag="WGN53_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ0140361.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGESDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFSDGISLTGDDRTLLQTLIRQLGVSLASAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENISFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            7102..7758
                     /locus_tag="WGN53_001377"
     CDS             7102..7758
                     /locus_tag="WGN53_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0140362.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1983 a         2368 c         1890 g         1833 t
ORIGIN      
        1 agttttgagg acgataccca atccaagctt tgcaacagcc gccgccatat ccgctataat
       61 tcacgcttca gccatcctgc ccccgacata aaatcatgac cctgaaaacc gatttattgc
      121 ctaaaatcaa caacgaagat tatcaacgcc tcatcctcaa acacagtgcg gaatttagcg
      181 gtggcgaaat ccgcctgttg aacgaaatcc tcgaaaaatt caatttcgac gttgttcagg
      241 cgcaggcatt ggcgcaagcc gtcatgcagc aaatccgctt cgaccccaac gcctaccaca
      301 tcgacagcga cgacgaagac accaccggca tctgccccca ctgcatcaac ccgcctatgc
      361 cgcccctgcg cgactatctc gtttggcgcg aaacccgcgg ataaaacgct tttgaccgtt
      421 atcttttcaa tgccgtctga aacgccgccg accgttcgga cggcataccc gacaaaggga
      481 acactatgct gcaaaccgac aacctgaccg ccgcgcaacc gcaacgcatc gttgccgccc
      541 aaaccgcctc cgcacaggaa gaactgctcg aacgcgccct ccgccccaaa acgctggacg
      601 actacatcgg gcaagacaaa gccaaagaac agcttgccat tttcatccaa gccgccaaaa
      661 aacgcggcga agcactcgac cacgttttgc tcttcggccc gcccggactg ggcaaaacca
      721 cactggcgca catcatcgcc aaagaattgg gcgtaaattt gcgccaaacc agcggccccg
      781 tcctcgaacg cgcaggcgac ctcgccgccc ttttgaccaa ccttgatccg cacgatgtat
      841 tgttcatcga cgaaatccac cgcctcagcc ctgttgtcga agaaatcctc tatcccgcgc
      901 tcgaagacta ccggctcgac attatgatag gcgaaggacc cgccgcccgt tccgtcaaaa
      961 tcgacctgcc gcccttcacg ctcatcggcg cgaccacccg cgccggtatg ctgaccaatc
     1021 cgttgcgcga ccgcttcggc atcgtctccc gccttgagtt ttacgaaaac cgagacctta
     1081 ccaccatcgt cagccgttcg gcacaactgt tgcagctcga tatgtccgaa gaaggcgcgg
     1141 aagaaatcgc caaacgcagc cgcggtacgc cgcgcatcgc caaccgcctg ttgcgacgcg
     1201 tgcgcgattt cgccgacgtg aaaaacaacg gcacaatcga cggcggcatc gccgatgccg
     1261 ctttaagtat gctggacgtg gacgcgcagg ggctggacgt gatggacagg aaatttctcg
     1321 aagccgtttt gcacaaattc ggcggcggcc cggtcggttt ggacaatgtt gccgccgcca
     1381 tcggcgaatc tacagacacc atcgaagacg ttatcgaacc ctaccttatc caacaaggct
     1441 tcctgcaacg caccccgcgc ggcaggatgg cgaccgaacg cgcctacctg catttcgggc
     1501 tgcccgtcga aaaataacgc aatgccgtct gaaacagagc taattttcag acggcatttc
     1561 tatttcaatc attggcgcaa ggttcagcct gccgcttttt tccagttccg ccctcatcgc
     1621 atcaatcacc gccttatagt ctggtttgcc gaaaatcgca gaaccggcaa caaaggtatc
     1681 cgcaccagct cgggcaacgg cggcaatatt gtcggttttg atgccgccgt ccacttcgat
     1741 ggcgatgtgc cgcccgcttt gtgcttcgta ccgatccagc atcgcccgca cccggcggat
     1801 tttttcaagg gtgtgcggga tgaagctttg tccgccgaat ccggggttga ccgacatcag
     1861 caaaaccata tccagcctgt ccaatacgtt ttccaacaga tatacgggcg ttgccggatt
     1921 caacaccagc cccgcctgac agcccatatc acgaatcagg ctcaagctgc ggtcgatatg
     1981 gcggctcgct tcgggatgaa aagtgataat cgatgctcct gctttggcaa acgactgaat
     2041 caggtcgtca acgggttcga ccatcagatg cacatcgatc ggcacgcttg cataaggctt
     2101 caacgccgcg caaaccatag ggccgaaggt caggttcggc acataatggt tgtccatcac
     2161 gtcaaaatgg atcagatctg cacctgccgc aatgacgctt tccacctctt ctccgaggcg
     2221 ggcaaagtct gccgacaaaa tactgggtgc gatacggtag gcggtcatat tttttccttc
     2281 aatgccttta cgggcggact caacgcggga aagccccgaa acagcattat taacaaaagt
     2341 taactgccat aataccatct ttcacacggc aatcaagtat attgtccgaa atatctggat
     2401 taaaggaaaa gccgtgccac tgcctgctcc ctgccgtttt gccaaacctg ccgcctcttt
     2461 tttaagtatg gctttgcttt cctgtcagct ttcccacgcc gccacggctt atatcccccc
     2521 gaacgatttt caaccgaact gcgacatacg ccgactcggg ctgacccaaa gtcagcacaa
     2581 tgagctgcgt aaaatccgca ccgccttcaa aatggcgggc gacagggcgc gtttgaaggt
     2641 tatgcattcc gaacacagcc gccgccggtc tgtcgtcgaa atcatttcct cggatgtttt
     2701 taatcggaac gaggcgcgcg attatgtcga aagccgctat ttgtccggta tggattttgc
     2761 ggtggacgaa ttggaaatcc aacaccggtt cttccatatc ctcacaccgc aacagcagca
     2821 aatgtggctt tcttcctgcc tcaaataatc cccgaaacgc tcacaacgcc cgttgtttcg
     2881 gcagcctgcc cgcccagtcg caggcaaact gccacgcgga acgtcccgaa cggctgcccc
     2941 gcgtctgcgc ccactgcaat gccgccatct gtgcggtttc atcatacggc acgccgaaat
     3001 cttccagcca attttgcacc gccgccagat aatcgttttg atcgaacgga taaaaactga
     3061 gccacaatca gaatcggtcg gacagggata ttttttcttc caccgcttct ttctgatgga
     3121 tttccccccg catccccgtc gtaccggcat tctcgtcaaa atattcgggc atcaggtgcc
     3181 gtctgttgga agtcgcgtaa accaaaacgt tggcgcaacg ttgagacaga ccgccgtcta
     3241 acgcggtttt caatgcctta taggtttcat cgccgctttc aaacgacaaa tcgtcgcaaa
     3301 atacgataaa tttttccgga cgttccttca aaagcgtcaa caggtaaggc aggccgatta
     3361 aatcgctttt atcgacttcg atcaggcgca atcccttatc cgcatattcg tgtagcaggg
     3421 ctttgaccag cgaggatttg cctgttccgc gcgcgccgct catcaataca ttgttcgcgg
     3481 gtctgccgac aatgaactgt tcggtattac gcaccagcaa ttcggtttgc ctgccgactc
     3541 ccgccagcct taccaaggga aaggtgtgcg gatcgggcaa gtgttccaaa aaaccttttt
     3601 tgcccgcact ctgccagcgg aaggcaagcg cgttccaatc cgtatgcccg ggttcgggcg
     3661 gaagcacggc atccaaacgc cgcaaaacgg cataggcttt atcgagaaat tcgttcaatt
     3721 ccatctctgc ctcactttgc atatctttgc gccatcagcc gttcgacggt atcgacgatt
     3781 gcctgcgtat tcggatcgat ttcgatgttg atcctgtcgc cgacctttct gctgccgaac
     3841 agcgtccgtt ccaaagtttc gggaatcaga tggacattga aacggccgtc ttcgactttg
     3901 cctatggtca ggctgcaacc gtccaagccg acgaaccctt tggtcaggat atagggtttg
     3961 agttcatgcg ggagcgaaaa ccaaaccgtg cggttgaacc cgtcccgttc gatttcgaca
     4021 ataggcacgg ttgccataat gtgtccgctc atgacgtgtc cgccgatttc gtctccgaaa
     4081 cgcgccgccc gttcgatgtt gacgcaatcg ccttccttca gcagccccaa attggttttt
     4141 gccaaagttt ccgccattaa atcgaaactg acgcggtttc cttcgatttc ggtaatcgtc
     4201 aggcagcagc cgttattggc gaccgatgcg ccgcgttgca gattgtccgc cgcctcttgc
     4261 ggaagctcga cgacataagt gtgaaatgcc tccgacgggc ggtggattgc cgtcagtttt
     4321 cccaatcctt gaacaatgcc tgtaaacata atcctgtttc cctgtgtcgg taaaaatggt
     4381 gcaaattgta gcatctcccc gcgaaaaatg ccgtctgaaa tgccttcaga cagcattatg
     4441 cctccgattc gggcaaaaac cgcccggtat ggcttgacct ttcctttcca cgccggtcgg
     4501 cggtcttgcc cttatccctc ctgcaaatcg atttgcgtgt tcaagtcggc aaaatgcccg
     4561 tcaaactcga atctgaccgg ccgcgccctt tgctgctgca accagcttct taatgttttc
     4621 atacccgaaa acagataggg aatcgcgctt tgcagaatct gcgggcggat atacataatg
     4681 ttgtagtgca tcgttatcgg cgtttccaca taatacgcat tgcacaacgg tgtgcgtttc
     4741 gacacggttt caaacctcgc caccaaatcg cccggcagat acggcatatc gcacggcaca
     4801 accaaaagcc agtcggcagt cgccaactgc aaatcgttgg ctgcggtaca caatgccgaa
     4861 agcgggccga aatgctgcca ctgccgtgcg tccggaaaaa tatgcggact tcttcgagca
     4921 tattcttcca aattccggtt ggtgctgatg gcgatatggc tgacctgcgg cctgaccctg
     4981 tcgatgacat gatctatcag tgccttaccc ccccaaaaga gcaagcccct tgtcctcgcc
     5041 tcccatacgg ctcgcctgac cgtcggccag tatcagggca aaagttttca ttgcggatgt
     5101 tctcttggaa aagttcgagg ttttcatgat tgcagtctgc cgttcccaat gactcaaaat
     5161 gccgtctgaa gcagacggca aataaattca tattatctga attttatcat aacatgattt
     5221 aacactgaag cggtacggat tatgttttcc ttaaccaatt ctttctgagc agttgtatct
     5281 aatcccaaag aatgatattg tttgcattat ttagaacaat ttttcgccga gcatgatact
     5341 gccagcccgt ttttcagacg gcatcagcct ttccctacgc ctgaaactcc tgaccggagt
     5401 gtgggtcggg ttggcggcat tgtctgtcgt tttgacactg ctgctctctt tgcgtctgga
     5461 aaacgcggcc tccgtcatcg aagaggcggg caacttgaga atgcaggcat accgtctggc
     5521 atacatggcg ggtgaaggat ctccccgctc ccaaatagac aatcaggttg acgaatttga
     5581 aaaaagcctg aatcgtattt cacaaagtga tgccatccat ccgctgattc cttcggacac
     5641 ccctcttgct tatgatttga tacaatccat gctgattata gattggcagg cacacatcct
     5701 ccccccgctc cagtcctacc ggcgaccgac tcaggtcgat ctctaccgct ttgccggaaa
     5761 catcgaactg tttttgcagg cattggaaaa tgccaacgaa aaaaacacat ggtggctcag
     5821 gcgttttcaa tgggcaatta tgttgatgac gctggtgtcg tctgtactga tgctgttttg
     5881 gcaccagatt tgggttatcc ggccgctgca ggcgttaagg gaaggtgcgg aacgcatcgg
     5941 acggaggtgt ttcgatattc cggttcccga aggcggtacg ccggaattca aacaggtcgg
     6001 gcgttgtttc aatcaaatgg gcggcaggtt gaaaatttta tatgatgatt tggaaggaca
     6061 agtcgccgag cagacacgca gtctcgaaaa acaaaatcaa aacctgaccc tgctgtacca
     6121 aactacacgg gacctgcacc aatcctacat accgcaacag gctgcagaac attttctaaa
     6181 ccgtatcctg cccgccgtag gagcagattc cggcagagtt tgtttggacg gcgaatccga
     6241 tgtttatgtt tccattcatc atgcggattg cggcacagca gcttcggatt tggggaagta
     6301 ccatgaggaa atcttcccca ttgagtacca gaacgaaaca ttgggcaggc tgttgctcag
     6361 cttttcagac ggcatttccc tcaccggaga cgaccgcacg ctgcttcaaa ccctgattcg
     6421 gcaattgggc gtatcgctcg ctagcgcgaa gcaggaagaa gaaaaacgcc tgcttgcagt
     6481 attgcaggaa cgcaacctga ttgcgcaagg attacatgac agcatcgcac aagcattaac
     6541 gttcctaaac ctacaggtac agatgctgga aaccgccttt gccgaaaaca aacgggagga
     6601 agccgcagaa aacatcagct tcatcaaaac aggcgtgcag gaatgttatg aagatgtccg
     6661 cgaactgctg ctcaacttcc gtaccaaaat cagcaataaa gaatttcccg aagccgttgc
     6721 cgacctattc gcccgcttta cgcaacaaac cgggataacg gtcgaaaccg cctgggaaaa
     6781 cggttcgttc ctgccgcctc aggaagcgca gctccaaatg atttttatcc tgcaggaaag
     6841 cctgtccaac atccgcaaac acgcccgcgc cacccatgta aaattcaccc tttccgaaca
     6901 cggcggacgc tttaccatga ccatccaaga caacggacaa ggtttcgaca cggagaaaat
     6961 aggagaaccc acgggcagcc atgtcggact gcacatcatg caggagcgtg ccaaacgcat
     7021 ccatgccgtt ttagaaatcc gttcccaagc tcaacaggga accaccgtct cattgacggt
     7081 tgcatctgaa gaaagcttga aatgactatt aaaattattc tgatagacga ccataccctc
     7141 ttccgcagcg gcattaaagc ccttttgtcg cgccaacacg gttttgaagt catcggcgaa
     7201 gccgcagacg gcctctcggg catcaaaatg attagtcggc tgcaacccga tgtcgtcctg
     7261 ctcgatttgg atatgcccgg catgaacgga cgcgaagcac tctcccaaat catcagcatc
     7321 aatccgcagc aggcagtgat tatgctgacc gtttccgaag acagcgacga cttgaccgaa
     7381 tgtattcgca tcggcgcgcg cggttacctg ctgaaaaaca tcaacgctga ctttctgctc
     7441 gaaagcatac gcaaagccgc cgaaggcgat aatgtattct cgcccgagat gaccgccaaa
     7501 ctcgttaaaa gcctgatttc cccccaacct gcccaaggga cacaggcact ctcctcactt
     7561 acccctcgcg aactggaaat cttgggctat ctcgccgcag gacacagcaa caaaatcatc
     7621 gcccgccacc tcgatcttgc cgaatccacc gtcaaagtcc acgttcaaaa cctgctccgc
     7681 aaactcaacc tcagcagccg ggtgcaggcc gctgtttacg ccatccggca caacgtcccc
     7741 caacccgtgc tggaataagg cgggttcaga cggcatatta ggggttttaa tccccgtacg
     7801 gtcattcgga taacagacca agcatgtaag tttatgcccc cataagtacg cttggcatag
     7861 cagtaatatt gttcggttta gtgttttccg tttgccccta tctgatactg caatatcagc
     7921 tatgccgtct gaaaacgtat catcatgata ttttcagacg gcataataaa aagcggaaat
     7981 actaatgcgg ggtaaaatgt tccattccga atcccataaa tatacaatgg cttatatcgt
     8041 ttaagcatgt gtaacccacc ctgatatcaa tcaa
//
LOCUS       DAWEWP010000088         7891 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWEWP010000088 DAWEWP010000000
VERSION     DAWEWP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.088"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            297..1610
                     /locus_tag="WGN53_001378"
     CDS             297..1610
                     /locus_tag="WGN53_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ0140363.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1833..2008
                     /locus_tag="WGN53_001379"
                     /pseudo
     CDS             1833..2008
                     /locus_tag="WGN53_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2004..3254
                     /locus_tag="WGN53_001380"
     CDS             2004..3254
                     /locus_tag="WGN53_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0140364.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVRGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGMEQQGTGLGLSIADTLATEYGRYL
                     ELSDSRRFGRGLLIRALLDKESLK"
     gene            complement(3288..4781)
                     /gene="rng"
                     /locus_tag="WGN53_001381"
     CDS             complement(3288..4781)
                     /gene="rng"
                     /locus_tag="WGN53_001381"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ0140365.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIDLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            4994..5251
                     /gene="grxC"
                     /locus_tag="WGN53_001382"
     CDS             4994..5251
                     /gene="grxC"
                     /locus_tag="WGN53_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ0140366.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5272..5715
                     /gene="secB"
                     /locus_tag="WGN53_001383"
     CDS             5272..5715
                     /gene="secB"
                     /locus_tag="WGN53_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ0140367.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5801..7843
                     /gene="recG"
                     /locus_tag="WGN53_001384"
     CDS             5801..7843
                     /gene="recG"
                     /locus_tag="WGN53_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ0140368.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTATETLEQLQTALPELNIG
                     LVHGRMKAAEKAEVMAQFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLSELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQAREIAPILIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1660 a         2054 c         2324 g         1853 t
ORIGIN      
        1 atcccgcgtg tacggcacgc cccgcgctcc gcaatgatag gggcggtttc gtttgaatcg
       61 gcgcaccggg atgaatgccc ccgcgccgtc ccgataccgg tttgccggtt gttccgtatc
      121 ggttgccgcc gtgaaatgac ggacggatac aggaatggcg gcaggcgtaa atgggaggaa
      181 atgaccgatt gacttgtttc aaaataaaaa agtagaatgc aaggcttatc ccgcgtctgt
      241 gggcggtttt gcctgcacgg tcggttttct gtttaaaata aaataaagga gaaatgatgc
      301 tgacgtttat cggactgctg attatcgggg tcatcgtatg gttgctgctg acggaaaaag
      361 tgtcgcccat catcgcatta atcttggtgc cgctgtttgg ggcgttgctg gcggggtttg
      421 atgtatccca attaaaagaa ttttattcgg gcggcaccaa atcggtgatg cagattgtga
      481 ttatgtttat gttttccatt ttgttttttg gaatcatgaa cgatgtgggg ctgttccgtc
      541 cgatgatagg cggtttgatt aagctgactc ggggtaatat cgtggcagtg agtgtgggga
      601 cggtcttggt gtcggtggtg gcgcagttgg acggggcggg tgcgacgacg tttttattgg
      661 tcgtccccgc ccttttgccg ctttacaagc gtctgcatat gaatccttac ctgctgtttt
      721 tgctgctgac ttccagtgcg ggattgatta accttctgcc gtggggcggg ccgactgggc
      781 gggttgcaag cgtgttgggc gcagatgtgg gcgaattgta taaacctttg ttgacggtgc
      841 aaattatcgg tgtagtgttt atccttgcgc tgtccctgct tttgggtgtg cgtgaaaaaa
      901 ggcggattgt ccgggagttg ggcgcgttgc ccgccgtggc ggatttgata aagccggtgc
      961 ctttgtcgga agaagaacaa aaattggcgc gtccgaaact gttttggtgg aatgtcctgc
     1021 tgtttttggc ggcgatgagc ctgctttttt cgggcatctt cccgccgggt tatgtattta
     1081 tgctggctgc aacggcggcg ttgcttttga attaccgcag cccgcaggaa cagatggagc
     1141 ggatttatgc ccacgccggc ggcgcggtga tgatggcgtc cattattttg gcggcaggta
     1201 cgtttttggg gattttgaag ggtgcgggga tgttggacgc gatttccaaa gaccttgtgc
     1261 atatcctgcc ggacgcgctg ctgccttatc tgcatattgc catcggtgtg ttgggcattc
     1321 cgcttgagtt ggttttgagt acggacgctt attatttcgg actgtttccg attgtggagc
     1381 agattacctc gcaggcgggc gtggcgcctg aagcagcagg ttatgcgatg ttgatcggca
     1441 gtatcgtcgg cacttttgtt acgccgcttt cgccggcttt gtggatgggc ttgggtttgg
     1501 cgaaattgtc gatgggcaaa cacatccgtt attcgttttt ctgggcgtgg ggtttgtcgc
     1561 tggcgatatt ggccagttcg atagcggcag gaatcgtgcc tctgccgtaa acggcgaagc
     1621 cgtctgaagc ccgaatcgtt cctgtggtct gtcgcaggct ctgcctgcct gcccgtgtgt
     1681 tgcaatcgcg ccccaatgcc gtctgaaggc agttgccggc gcggtttttt tgtgctgcag
     1741 ttttgaaaaa taccggcaag cgtaaagacc gctaaattgc ggctaatttt ggggtgttag
     1801 aataggcacg gtttattttg aagggaaagt tgatgcgtgt tttgctggtg gaagacgatg
     1861 cgatgattgc ggaggcggtg tcggcaagtt tgaaagacgg cggctatgcg gtggattggg
     1921 tcaaaaacgg cgcgcggttg ccgctgccca gccttatgac ttgatgctgc tggatttggg
     1981 tttgccgggg cgggacggtt tggatgtttg ttccgctggc aatgcttgca ggtatgtttt
     2041 cctactacga aaccttccac gaaacggaag cgttgcagga cgacctgctc cgtcaggcgg
     2101 cattgtacgt cgcccccgat tccaaacccg aaactttgcc cgagggcgac ggcgatacgc
     2161 gtattttggt acagatgccg cagcaggaag accctgttgt cagcctgcct gcgcatctgg
     2221 cggacggtct gcacacgctt caggcggacg gggacgacga ttattaccgc gtctatatcc
     2281 gcacgaccga acagggacgg attgccgtta tgcaggaaaa cgaataccgt gaagatttgg
     2341 cggcggatgc ggcacggcaa agcgtgttgc ccctgctggc ggcactgccg ctgatgatac
     2401 tgctgaccgt gtggattacg cacaaagcca tgcgccccgt ccgcaaattg tcgcaaagtc
     2461 tcgaacaacg ccgaatcaat gacctgtctg ctttgagtgt ggacaatatt cccagtgaaa
     2521 tcagagggtt cgtaaccgcc atcaacctgc ttttgaaacg tgcggatgaa gacatccgcc
     2581 accgtcagcg gtttgtcgcc gacgcggcac acgaattgcg tacgccgatg acagcccttt
     2641 cccttcaggc ggaacggctc aacaatatgt cgctcccacc cgatgcagcg cggcagcctg
     2701 ccgttttgca gcagagcatc aggcgcaaca aacacctgct cgaacagctt ttggcactgg
     2761 cgcgttcgca gtcggacgaa acccctctga cgaaaacgac attcggtctg caaagccgtt
     2821 tccgccaagt gttgcaggaa ctgatgccgc tggctttgga aaaacgtcag gacatcggtg
     2881 tggcggtcag aggcgatgtc gaagtgtctg ccgacgaaac agaaatctat acgctgatta
     2941 aaacctttgc cgataacgcg gtacgttaca cgccgcctga aggcaggata gatttgggtt
     3001 tcacggacga agggaaatat cttgccgtgt gggtggaaga taacgggaac ggcattcccg
     3061 aatccgaacg cgcccgcgtc ctcgatccgt tttaccgtat tttgggaatg gagcagcagg
     3121 ggacggggct ggggctgtcc atcgccgaca cgcttgccac agaatacggc agatatttgg
     3181 aattgtccga cagccgccgg tttgggcgcg ggctgctgat acgcgccttg ttggacaagg
     3241 aatccctgaa atagacagat gccgtctgaa ggcttcagac ggcattttta catcaaaacg
     3301 atgtcgtatt gttcctgcgt gtaagcggtt tcgactgcca gagaaatcgg tttgccgatg
     3361 aaatctatca gcattgccaa ggattgcgat tcttcgtcca aaaacaaatc gatgacgttg
     3421 ggggcggcga ggatgcggaa actttcggca tcgtaacggc gcgcttcgcg gacgatttcg
     3481 cgctggattt cgtagcatac ggtttgcggc gttttcagac ggcctctgcc ttggcaggaa
     3541 gggcagggtt cgcagaggac ttggtttaag ttttcgcgcg agcgtttgcg cgtcagctcg
     3601 accagtccca ggctggtaaa accgtgcagg gtaacgcggg tacggtcgaa ggcgagggct
     3661 ttggcaagct cctgcaacac ggcttcgcgg tggctttcct gcgccatatc gatgaagtcg
     3721 atgatgatga tgccgccgag gttgcgcagc ctcaattcgc gggcgatggt gtggcaggct
     3781 tcgaggttgg tgcggaaaat cgtttcatcg aaattgcgtg cgccgacgaa gccgccggtg
     3841 ttcacgtcta tcgtggtcat cgcttcggtg gattcgataa tcaggtagct gccgaagttg
     3901 aggttgacgc gcggttgcag ggcgcggctg atttcctgct cgacgttgtg ggtttcaaat
     3961 agcgggcgtt cgcctttgaa caattctatc ctgcccaatg cgccgtggac gtattgttcg
     4021 gcaaaacgcg tcatgcggcc gtggtttacg gtggaatcga cgaggatttt ttgcgtgtcg
     4081 cagccgacca tatcgcgcaa cacgcgcaaa ctcaagggca aatcctgata aagcagggtt
     4141 tccggcggcc ggattttcgc ctgttcttgg atgtgttccc acactttggt caggtagtcg
     4201 atgtcggact ggagctgttc gtcggtggcg ttttcggcgt tggtgcggat gatgtagccc
     4261 cggcaggcat tttccggcag gagcttgtcg aggcgttcgc gcaggctgct gcgttcggca
     4321 tcgtcttcga tgcgttggga cacgccgatg tggtcttctt gcggaagatg gacgaggaaa
     4381 cgccccgcca gcgagatttg ggtggaaagc cgcgcgcctt tggtgttgat cgggtctttg
     4441 atgacctgca ccaaaacaga ctgcccttca aacagcatat gttcgatgcg ctgggtttct
     4501 tcggggttgc ggcgttgttc gaggacatcg acgatgtgta aaaacgccgc gcgttccaag
     4561 tcgatgtcga taaacgcgct ctgcatccca ggcagcacgc ggcgcaccac gcccaaatag
     4621 atattgccga ctaggctgtg ttcgctgttg cgctcgatgt gcagctcgca gatattgttt
     4681 tcctccaaca ccgctacgcg cgtttcctgc ggcgtgatgt tgaccaatat cgtttcgggc
     4741 gggcgcgcga tgtctttggg gatggggagt cctgacaaca tggtttttcc tgaaaatgta
     4801 ataaaaatat tttgcatccg ttccgccgcg cagcgcgggt gcaaaaatat ttttcacaat
     4861 gtctatcata ctttaaaaaa gaaactttga cacattccgc acatgccgtt tgaacgtgca
     4921 tctgttccgc acttgccaaa caggggaaat gcccctatat tgattatcat tgcaaaactt
     4981 tcggaaaccc aatatgcaga ccgttaccat gtacacaggt ccgttttgtc cctactgcgc
     5041 gatggcgaaa aggctgctgc acgcggcagg tgtcggacat atcgacgaaa tccgtgtcga
     5101 tgcaagcccc gaagcctttg ccgaaatgca gcagctttcg ggacagcgca gcgtgccgca
     5161 gattttcatc ggcgaaacgc acgtcggcgg atttaccgac ctctaccgcc tccagcagga
     5221 aggcgggctg gacggactgc tgaaccctta acccccaact aggaaaacaa aatgagcgaa
     5281 gaactgcaac ccgtattcag catcgagcga ctgtatgtca aagacttgtc tttggaagtg
     5341 ccgcacgcgc cgcaaatctt tttggaacag ggcgagcccg aagtggatat gcgcgtttcc
     5401 accggcagcc aaaagctgga agacggctac tacaacgtgg atgtaaccgt taccgtaacc
     5461 gccaaattgg ataacgagcg cacgatgttt ttgaacgaag taacccaaag cggtattttc
     5521 cgtttggaaa acatccccga agaagatgtg cagctgctgc tgggcgtggc gtgtccgaac
     5581 atcctcttcc cttacgcgcg cgaagccgtt tccggtacgg taacacgtgc cggcttcccg
     5641 cccgtcctgc tcgcgccgat taattttgag gcgatttacc aacaacagca ggaagccgaa
     5701 gccgccgggg cttgattcct gcctgccgat gccgtctgaa tccgtttcag acggtatttt
     5761 tgttttcgga taaaataggt gcgttcaaag gaatctgcct atgatgtcgc ccgaaaccca
     5821 gaaacagctc aaaatcaccg atgtttccgc caagaagctc gacaaactca acctccatac
     5881 cgcgtgggat ttggtgttgc acctgccgct gcgttacgag gacgagacgc acattatgcc
     5941 gattaaggac gcgccgattg gcgtgccgtg ccaggtcgag ggcgaggtta tccatcagga
     6001 agtaacgttc aaaccgcgca agcagttgat tgtccaaatc gccgacggtt ccggcagcgt
     6061 cctttttctg cgcttcatcc acttttacgc cagccatcag aaacagacgg cggtcggcaa
     6121 acgcatccgc gccgtgggcg aaatcaaaca cggcttttac ggcgacgaga tgattcatcc
     6181 caaaatccgc gatgccgagg gcggcggttt ggcggaaagc ctcacgccgg tttacccgac
     6241 cgtaaacggt ttgaaccagc ccactttgcg ccgtattatt cagacggcgt tggacgttac
     6301 gccgctgcac gacacgctgc ccgatgccct gctgtgccgt ctgaagctgc cacacctcgc
     6361 cgaaagcctg cgtcttttgc attcgccgcc gccgagtttc acgattcatc agctttcaga
     6421 cggcacgctg cctgcatggc aacggctcaa attcgatgaa cttttggcgc aacagctttc
     6481 gatgcgcttg gcgcgacaga agcgtatcgg cggcacggcg gcggcattgg gcggcgacgg
     6541 cacattgacc caagccctgc gccaagccct gccgtttgcc ctgaccgatg cgcaagaaaa
     6601 agttgtttcc gaaatctgcc gcgatatggc gcaaacctac ccgatgcacc gcctgctgca
     6661 gggcgatgtc ggcagcggca aaaccattgt cgccgccttg tccgcgctga ccgccatcga
     6721 atccggtgcg caggtggctg taatggcgcc cactgaaatc cttgccgaac aacattttat
     6781 taaatttaaa caatggctcg aacctttggg cattgaagtt gtccgccttt ttggcagttt
     6841 gcgtaaaaaa gccaaagacg aagccaaagc caaactcgcc gacggcagcg tcaaaatcgc
     6901 cgtcggcacg cacgccctgt tttcagacgg cgtggcgttt cacaatttgg gcttgaccat
     6961 tgtggacgaa cagcaccgtt tcggcgttgc ccaacgcctc gccctcaaaa acaaagggcg
     7021 cgaagtccat cagctgatga tgtccgccac gcccatcccg cgcacgcttg cgatgagttt
     7081 tttcgccgat ttggacgtgt ccgtcatcga cgaattgccg cccgggcgca caccgattaa
     7141 aacgcgcctc gtcaacaacg tccgccgcgc cgaagtcgaa ggcttcgtcc tcggcacttg
     7201 ccgaaaaggg cggcaggcgt attgggtctg cccattgatt gaagagagcg aaaccctgca
     7261 actgcaaact gctaccgaaa ccctagagca gcttcagacg gccttacccg aactcaacat
     7321 cggcttggta cacgggcgca tgaaagccgc cgaaaaagcc gaagtcatgg ctcagtttgc
     7381 cgcaggccgt ctgaacgtct tggtcgccac caccgtcatc gaagtcggcg tagatgtgcc
     7441 caacgccgcc ctgatggtga tcgaacacgc cgagcgcatg ggcttggcgc agctgcacca
     7501 attacgcggt cgggtagggc gcggcgcggc agaaagcgtg tgcgttctcc tgtttgccga
     7561 gccattgagc gaactcgcca aagcgcggtt gaaagtcatc tacgaacaca ccgacggctt
     7621 tgaaatcgcc cgccaagacc tcaacatccg cggccccggc gaatttctcg gcgcgcgtca
     7681 aagcggcgtg cctatgctgc gtttcgccaa gctcgaagaa gacttacacc ttttggaaca
     7741 agcgcgcgaa atcgccccga tactgattga gcaaaacccc gaaatcgtcg aagcgcattt
     7801 ggcaagatgg ctttccggca gggaagggta tttgggcgtg tgagcaaaat gccgtctgaa
     7861 atattagaaa aaaaagtgta acggaaatct g
//
LOCUS       DAWEWP010000089         7749 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWEWP010000089 DAWEWP010000000
VERSION     DAWEWP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.089"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(13..1215)
                     /gene="trpB"
                     /locus_tag="WGN53_001385"
     CDS             complement(13..1215)
                     /gene="trpB"
                     /locus_tag="WGN53_001385"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ0140369.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1400..2110)
                     /locus_tag="WGN53_001386"
     CDS             complement(1400..2110)
                     /locus_tag="WGN53_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ0140370.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFGDADYFKVPSNGEAAHS
                     EKAFDAEKIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECR
                     HICTTGGKATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKK
                     AAAYRAFFEMAGLCEKQL"
     gene            2307..3086
                     /gene="rsmA"
                     /locus_tag="WGN53_001387"
     CDS             2307..3086
                     /gene="rsmA"
                     /locus_tag="WGN53_001387"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ0140371.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            3259..3987
                     /locus_tag="WGN53_001388"
     CDS             3259..3987
                     /locus_tag="WGN53_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0140372.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            4060..4419
                     /locus_tag="WGN53_001389"
     CDS             4060..4419
                     /locus_tag="WGN53_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140373.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDINKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            4424..4864
                     /locus_tag="WGN53_001390"
     CDS             4424..4864
                     /locus_tag="WGN53_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ0140374.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVSQCIIPDMTDKESYRRLKVWVLWRQPKKTAETDTPS"
     gene            4896..6170
                     /gene="folC"
                     /locus_tag="WGN53_001391"
     CDS             4896..6170
                     /gene="folC"
                     /locus_tag="WGN53_001391"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ0140375.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKVGTLTSPHLLRYNERIAVNAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFHPQHSDDPTRNRNALPFPALRGAYQLSNAACTLTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHGIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6183..7181
                     /gene="ftsN"
                     /locus_tag="WGN53_001392"
     CDS             6183..7181
                     /gene="ftsN"
                     /locus_tag="WGN53_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0140376.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETNGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKETKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            7174..7671
                     /locus_tag="WGN53_001393"
     CDS             7174..7671
                     /locus_tag="WGN53_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ0140377.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
BASE COUNT         1943 a         2202 c         1997 g         1607 t
ORIGIN      
        1 agacggacga ggttacagtt taatcccttt gagcttcgcg acggtattga tgtctttgtc
       61 gccacgaccg gataggttga ccagaatcac ttggtctttg cccattttcg gcgcgttttt
      121 caccgcccaa gcaacggcgt ggctggattc gagcgcgggg atgatgccct cgaagcggca
      181 gagcaagtca aaggcttcga gtgcttcgtc gtctttggca acagtgtatt cgacgcgctt
      241 gatgtcgtgc agatggctgt gttccgggcc gatgccgggg taatccaagc ctgcggaaac
      301 agagtgcgta cccaaaacct gaccgttttc gtcctgcatc agatagctgc ggaaaccgtg
      361 caatacgcca atcggtgcgc ccgaagtaat cggcgcggcg tgatcggggg tgttcacgcc
      421 caaaccgcca gcctccacgc cgacgaggcg cacgttttct tcgccgatat acgggtggaa
      481 caaaccgatg gcgttcgatc cgccgcccac gcaggcaacg gcaacgtcgg gctgtctgcc
      541 gatggcttcc tgcatctgcg ctttagcttc gttgccaatc acgcattgga aatcgcgcac
      601 catttcggga tacggcgcgg ggccggcggc ggtaccgatg atgtagaacg tgtcgtccac
      661 gcgggcgacc cattcgcgca tggcttcgtt catcgcgtct ttcagcgtgc ggctgccgct
      721 ttctacacca accacgttcg cacccaataa tttcatacgg aacacgttgg gcatttggcg
      781 ttggatgtcg tccgcgccca tatacacgtc gcaagtcata ccgaagcgtg cggcaacggt
      841 ggcactcgcc acgccgtgct gacccgcgcc ggtttcggcg atgacgcgtt ttttacccat
      901 gcgttttgcc aacagtgcct gaccgatggt gttgttgact ttgtgcgcgc cggtgtggtt
      961 caagtcttcg cgcttcaacc agatttgcgc accgcccaga tgttcggaca accgcgcggc
     1021 gtggtaaacg gggctgggcc tgccgacata atgtttcaaa tcatggcgga acgcttccca
     1081 aaattcagga tcgtttttcg ctgccttata ggcatccgcc agctcttgca aagccggaat
     1141 cagggtctcg gacacgaaca gcccgccgtg ttcgccgaaa aagcccttct cgtcgggcgc
     1201 gtggtagttc ttcatcagat ttcgtccttg tgcttcaaat tttcagacgg caatttatag
     1261 tggattaaca aaaaccggta cggcgttgcc ccgccttgcc gtactatttg tactgtctgc
     1321 ggcttcgccg ccttgtcctg atttttgtta atccactata tttttcaaac atgccgtccg
     1381 aacgggaaat tgtcggcaat tataactgtt tttcacacaa acccgccatt tcgaaaaacg
     1441 cccgatacgc cgccgccttt ttcgccaaac tcaaaggata ggcacgcgaa gtcgaaggca
     1501 ggcgcgtcag cgtcaaatcc cgtccggcaa acggaaacgg cacggtttcg cccgttttcg
     1561 gcattttgat gccgccacct tgaatatcga gcaggatttc cgtcgccttg ccgcctgtcg
     1621 tacaaatatg gcggcactcg ggtatttttg ccaacaccgc cgccaaatcg acggtttcaa
     1681 ctacctttaa aaacttgtcg gacgcattgc cgtgctgacg taccgccttc aaaacggtcg
     1741 gacaggacgc aatcccccgt tcgtgcaaaa acgccttgat tttctcggca tcaaacgctt
     1801 tttcagagtg cgcagcctcc ccgttggacg gcactttgaa ataatccgca tcgccgaaaa
     1861 acaccagccc gtaaacgcgc cacatatcgt tttggaaatt cggataatga aactgcatcg
     1921 cgcgtttgtc ttccttgggc ggaaacgtcc ccatcatcat gaccgcagcc ttcggcggca
     1981 acacggaatc gaagggatgg gtttcgattt caagcagatt ttcagacacg ttcaatcctc
     2041 caacaggcaa aacggcagac agccggtttt tgcaccaaac cgccaaacac ggcaagcctt
     2101 caaacagcat tatcacgccc tgccgttttc ggttacaatg ccgacatcaa acggatactg
     2161 taaacacatt tatgtttcaa cacacaggac gacacataaa gcaccgccct atgtgccatc
     2221 ctgatttgga aggggttacg cccctcccaa atagagtctg attctaccgc cctaaagggc
     2281 ggggtttcaa ccgaaaagga aacacgatga aagaacacaa agcccgcaag cgtttcgggc
     2341 agaatttttt gcaggacacg cggattatca gcgatattgt caacgccgtg cgcccgcagg
     2401 cggatgatgt cgtgattgaa atcggtccgg gtttggcggc gattaccgaa cctttggcga
     2461 aaaagctgaa ccgcctgcac gttgtcgaaa tcgaccgcga catcgtatgc cgtctgaaaa
     2521 cgctgccgtt tgcggataaa ctggtgattc acgaaggcga tgtattgcag tttgatttca
     2581 acggcatcgc aggcaaaaag aaaatcgtcg gcaacctccc gtacaacatt tccacaccgc
     2641 ttttgttcaa gctggcggag gtggcggacg atgtcgtcga tatgcacttt atgctgcaaa
     2701 aagaagtggt cgagcgtatg gttgccgcgc cgaaaagcaa cgactacggc cgcttgggcg
     2761 tgatgctgca atattttttc gatatggaaa tgctgattga cgtaccgccc gaatcgttcg
     2821 accctgcgcc gaaagtggat tccgccgtcg tccgtatgat tccggtgaaa caccgcatcg
     2881 gcaaggcgga cgatttcgag catttcgcca aactggtgaa actcgccttc caccaacgcc
     2941 gcaaaaccat acgcaacaac ctgaaagagc ttgcaggcga cgatgatttg caggctgtcg
     3001 gcatcaatcc gcaagaccgt gccgaacaca tcgcgccgga aaaatacgta gcgttgagca
     3061 attatctggc gggcaaggcc gtctgaaaag ggagtaagcc gatgaagaaa tacctgcttt
     3121 tacccctgat actgccgctt gcagcctgcc tgaccaatcc gaacgaccgt ttttttgacg
     3181 gcagacgcta cacgcttcat catatggagc tcccgtaacg acaacctgca acggcaaatg
     3241 ccgtctgaaa gaaaagacat gatcaaattc aaaaacgtac acaaacattt caaagacctg
     3301 cacgtcatca acggcgtgaa cttggaaatc aaaaaaggcg aagtggtcgt cgtctgcggg
     3361 ccttcgggca gcggcaaatc cacactcatc cgcaccgtca accagcttga aagcatcgaa
     3421 agcggcgaga tttgggtgga cggtgtcaac gttgccgacc caaaaaccga tttgaacaaa
     3481 atccgcgaag aagtcggctt tgtgtttcaa agtttcaacc tctacccgca cctgaccgtg
     3541 ttggaaaaca ttaccctcgc accgatgaag gttaaggggc aaaatgcgga acaggcggag
     3601 aaaaaggcaa tggagctttt ggaacgcgtc ggactggcac acaaaaaaga cgccttccct
     3661 tcccaacttt ccggcggcca gcagcaacgc gtggcgattg cacgcggttt ggcgatggaa
     3721 ccgcgcgtaa tgttgtttga cgaaccgacc tccgcactcg accccgaaat ggtcggcgaa
     3781 gtgttgaaag tgatgaaaga cttggcagaa agcggcatga ccatgatgtg cgtaacccac
     3841 gaaatggggt ttgcccgcga agttgccgac cgcgtgattt tcgtcgataa agggcaaatc
     3901 ctcgaagacg aaacgccgga agcgtttttt accaacccga aacacgaacg cgccaagcag
     3961 ttcctgcaac aggtcatgac ccattgatgc tatgccgtct gaaaaccgaa cgttcagaca
     4021 gcatatcccg acaccgcaag atgattgaga aagaaaacca tgtattttgt tgaccgcacc
     4081 gccgtcgtcc tcaagccgac cgcgcgcttt ttggaatggc tcaaaagcac cgacgaaaat
     4141 atgcccgacc tgaccataga gcagcttcgt gccaactgtt ccgtcttcct cgttccgcag
     4201 ttcgacgaac cggaagccgt cgtttcctac ttcgacgaac gctaccgcca gattttcgag
     4261 gcggagctgg caggttggga catcaataag gataaatggc cgcaagatat ggggctgaag
     4321 gcgttttggg aatttttcga catcgaaatc cacgatatgg tgttggacat ggaagaagcg
     4381 gaattgaaca ttacccccgt gttcgacaac atgatgtaag gctgtgcgcg cctttcagac
     4441 ggcattgagg ccgtcccgca tcctgaacat cctgaccgta tcgctccact cggcttccct
     4501 tgccgtctgc ctgacctggt tttacggccg gatgatgtgg ttcggtttgg cggctttggt
     4561 ggcaagttac gcatactcgc tcaggataac gaatctgaaa caccgccatg ccataaccgc
     4621 cattaccata gaccgcgacg gacaggcgga aatcgtatcc ggcaaagaca aaacagcggc
     4681 aacacttgcg ggcagcagta tggttacgcc ttatgcctta ttcttacaat gggacaccgg
     4741 cggcaaaacc gtcagccaat gcatcatacc cgacatgacg gacaaagaat cctaccggcg
     4801 gctgaaagtc tgggtgcttt ggcggcagcc gaaaaaaacc gccgaaaccg atacaccaag
     4861 ctgatttcag acggcatttg aacacggaac actcaatgaa aacactgcaa gactggctct
     4921 cccatttgga aaccgcccac agcggtggct tgatcgatat gggcttggaa cgcgtaggcg
     4981 aagtgaaaaa acggatgaag ctcacgccgc aatgccccgt cgtcgttgtc gcgggaacaa
     5041 acggcaaagg ttcggtctgc gcctacctga cacagattta caaacaggca ggctttaaag
     5101 tcggtacgct gaccagcccg catttattgc gttacaacga acgcatcgcc gtcaacgccg
     5161 aacccgtttc ggacgatacg attattgcct ctttcgagcg catcgaagcg gcgcgcggcg
     5221 aaatatcgct gacttatttt gaattcaatg ccttggcggc cgtcgacatc ttcatgcgcg
     5281 aacaggttga cgtgatgata ttggaagtcg gcttgggcgg acgcttggac gcggtcaacg
     5341 catttgacgg cgattgcgcg gtggttacca gcgtggattt ggaccatcag gcgtttttgg
     5401 gcgatacggt cgagcaggtc ggctttgaaa aagcaggcgt gttccgcagc ggcaaacccg
     5461 ccatctgcgg tcaaaacccc gcgcccgcat cattggtcgc acacgccgaa gccataggcg
     5521 cgaaactgct gatggtgcag cgcgatttcg agtttcacgc gctggaaaac atccaatgga
     5581 actaccgctt ccatccgcaa cattcagacg accccacgcg caaccgcaat gccctgccct
     5641 tccccgcatt gcgtggcgca taccagcttt ccaacgccgc ctgcacgttg accgtattgg
     5701 aatgcttgaa cgacaaattg cctgtggaca tcggcgccat caaacgcgga ttgctgctgg
     5761 ttgaaaatcc cggccgcttc caagtcctgc ccggccgtcc gctgaccgtt ttggatgtcg
     5821 gacacaatcc ccacgctgcc cgcgccctgc gccgcagcct gattaatttg gcgtttgcgc
     5881 aaaaacgcac cgccgtattc agtatgctgt ccgacaaaga catagacggc gtgttggaaa
     5941 tcgttaaaga ccagtttgac gagtggtata tcgcaccgtt ggatgtaccg cgcggcatga
     6001 cgctcgagag cctgcaaaac aaactgcacg aacacggcat cgaaaacata cagacattcg
     6061 ccgccgtccg cgaagcatac cgggcggcag cctcaaaagc gggcgagaac gacagaatcg
     6121 tcgttttcgg ctcatttcat accgttgccg atgtcatgtc ggtactttaa aaggaaactt
     6181 ctatgtccga aaacaaacaa aacgaagtcc tgagcggtta cgaacaactc aaacggcgca
     6241 accgccgccg cctcgtaacg gcaagttgcc tggttgccgc ctcctgcatc ctgctggcag
     6301 ccgccctcag ttccggccct gccgaacaga ctgccggcga aacaaacggc gtagaaaaca
     6361 aagcggcagg tgcggcacaa acccctgcct tgaaatccgc cgccgacaaa ccgcaggact
     6421 tggcaggcga agacaagcct tctgccgccg acagcgaaat cagcgagcct gaaaacgtag
     6481 gcgcgccgct ggtgctgatt aacgaccgcc tcgaagacag caacatcaaa ggtttggaag
     6541 catccgagaa actgcaacag gcagaaaccg ccaaaaccgc accgaagcag gcaaaacaac
     6601 gcgctgccga aaaagtgccg gcaactgccg acagtacgga tacggtagcg gttgaaaaac
     6661 cgaaacgcac tgccgaaaca aaaccgcaaa aagcggaacg cactgccaaa gccaagccca
     6721 aagccaaaga aaccaaaacc gccgaaaaag ttgccgacaa accgaaaact gccgccgaaa
     6781 aaaccaaacc ggatacggca aaatccgaca gcgcggtaaa agaaacgaaa aaagccgaca
     6841 aggctgaaag caaaaaaaca gccgaaaaag accgttcgga cggcaaaaaa cacgaaacgg
     6901 cacaaaaaac cgacaaagcg gacaagacca aaaccgccga gaaggaaaaa tccggtaaaa
     6961 aagccgccat tcaggcaggt tatgccgaaa aagaacgcgc cttaagcctc cagcgcaaaa
     7021 tgaaagcggc gggtatcgat tcgaccatca ccgaaattat gaccgacaac ggcaaagttt
     7081 accgcgtcaa atcaagcaac tataaaaacg caagggatgc cgaacgcgat ttgaacaaat
     7141 tgcgcgtaca cggtatcgcc ggtcaggtaa cgaatgaata acctccccgt tgccgacctc
     7201 ctcgtcttcg ccatcatcgc cgcctgcatc gtgctatccg cgatgcgcgg cgtgattgcg
     7261 gaggcaggtt cgatggcggc atgggtggtt tccttctttt ttgccaaact ctttgccgca
     7321 cccttcgccg acctcgcctt tgcctcgttc caaccccgcc tgtttgcatt ggctctgtcg
     7381 ttcatttccc tgttcgtcat tgcctgtctg atccagaaaa tgctccgttc gctgctgacc
     7441 ggcgcagttt cggcggtcgg tttgggcttt gccaaccgca ttttgggcgg cgtattcggt
     7501 gcattgaaag gcattttgat tattaccctg ctggtcatgc tcgcttcaaa aaccgacctg
     7561 cccgataccg aagaatggcg gcaatcttac acactgccgt ttttcgtatc gctttccgaa
     7621 gccgtgttga accatagcgg cggcacggcg gaaactccgg aagacgattg attgccctgc
     7681 tggcgtttca gacggcattc tcttttatgc cgtctgaaat gttttttcct ttcctcagcc
     7741 atatcacag
//
LOCUS       DAWEWP010000090         7732 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWEWP010000090 DAWEWP010000000
VERSION     DAWEWP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.090"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="WGN53_001394"
     CDS             complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="WGN53_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ0140378.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1556..1711
                     /locus_tag="WGN53_001395"
     CDS             1556..1711
                     /locus_tag="WGN53_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140379.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1708..2139
                     /locus_tag="WGN53_001396"
     CDS             1708..2139
                     /locus_tag="WGN53_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ0140380.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2273..6637
                     /gene="app"
                     /locus_tag="WGN53_001397"
     CDS             2273..6637
                     /gene="app"
                     /locus_tag="WGN53_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ0140381.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFTYKIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFHD
                     NNNGTGKIDAKHEHNSLPNRLKTRTVQLFNVSLSETAQEPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTNTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     DLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSLLSVTPPASVESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAG
                     RDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPATTAFP
                     RARRARRDLPQLQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDR
                     RNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDD
                     GIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGSKIRRRVLHYGIQARY
                     RAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHI
                     SITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAK
                     GPQLEAQHSAGIKLGYRW"
     gene            complement(6746..7468)
                     /locus_tag="WGN53_001398"
     CDS             complement(6746..7468)
                     /locus_tag="WGN53_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0140382.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASVSLEDIQTILNLKRAYQYVISHISNGEPVNISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGIDYFGTHEDIDFPDPPNMG"
BASE COUNT         2067 a         2154 c         1869 g         1642 t
ORIGIN      
        1 aaccaaacgg atcggattcc cgtttttatg ggataacgga atgttcagcc ggacaccgcc
       61 acgatgcttt cgatccgttt attttccgat acaaaacctc gaaaaaatca cgccgagcag
      121 gtcgtccgcc gtaaactcgc ccgtgatttc gccgcatgcg acctgcgcca agcgcaagtg
      181 ttcggcaaac agctcgattt gatggttgcc gcacaatgcc gccagcgaca attcttcctg
      241 cgctgctttg agtgcgttga cgtgccgcgt ccgcgccaaa aacagccctt cgccctcgcc
      301 ctgccatccg gcctcgcgca acaacgtccg tttcagcgcg tccaagccgt cgccggtttt
      361 cgccgacagc gcgatgacgg tttccgcgcc cgtaccgaac ccgcctgccg cgtgtgcgtg
      421 caaatcggat ttgctgtgga tttcgatgcg tttcaactcc ggcggcaacg cgtccaaaat
      481 cgcccgtgtc ttgtcgttca cgccttcgcg cggatcgacc aacaccagcg cgacatcggc
      541 ttcggatacg gctttgcggc tgcgttcgat gccgatgcgt tcgaccacgt cgtccgtctc
      601 gcgcaaacct gccgtatcga caatatgcac cggaacgccg tcaatcagga tacgttccct
      661 gaccgcgtcg cgcgtcgttc cggcaatatc ggtaacaatc gccacttcgt cgcccgccag
      721 cgcgttcaac aggctggatt tgccgacatt cggcgcgccg accaatacga cattcagacc
      781 ttcgcgcaaa atcgcgccct gctgcgcgtt ggcaagcaca tcatccacgg cgcggcgcaa
      841 gccgtccagt ttgccgcgtg cgtctgccgc ttcgagaaaa tcaatgtctt cctcgggaaa
      901 atctaacgtc gcttcgacca gcatccgcaa ggtaatcaag tcttcgacta gaccgtgtat
      961 ccgccgcgaa aaatcgcctt tgagcgagcg caacgccaga cgcgccgccg aacggctgga
     1021 tgcgtcaatc aaatccgcca cgccttccgc ctgtgccaag tccagtttgt cgtttaaaaa
     1081 cgcacgcttc gtaaactcgc ccggttcggc aaggcgcgcg cccaattcca aacagcggtt
     1141 cagcagcata tccatcacta ccggcccgcc gtgtccctga agctcgataa catcttcgcc
     1201 cgtaaaactt gccggtgcgg caaaaaaaag caaaagcccg ctgtcgattg cctgaccgtc
     1261 cgcgtccgta aaatcagcat aggttgccac gcgcggcttg ggcgttttcc cacacaaagc
     1321 ctctgccatc ggcagcaggt ttttccccga tatgcgtatc acgcccacgc cgccgcgccc
     1381 gggtgcggta gcgactgccg caatcgttgg aacgttatcc gacataaaac ccccgaaaat
     1441 tcaaaacagc cgcgattata gcaaatgccg tctgaagtcc gacggtttgg ctttcagacg
     1501 gcataaaacc gcaaaaatgc ttgataaatc cgtccgcctg acctaatata accatatgga
     1561 aaaacgaaac acatacgcct tcctgctcgg tataggctcg ctgctgggtc tgttccatcc
     1621 cgcaaaaacc gccatccgcc ccaatcccgc cgacgatctc aaaaacatcg gcggcgattt
     1681 tcaacgcgcc atagagaaag cgcgcaaatg accgaaaacg cacaggacaa ggcgcggcag
     1741 gctgtcgaaa ccgtcgtcaa atccccggag cttgtcgagc aaatcctgtc cgacgagtac
     1801 gtgcaaataa tgatagcccg gcgtttccat tcgggaccgt tgccgccgcc gtccgacttg
     1861 gcgcaataca acgacattat cagcaacggg gcagaccgca ttatggcaat ggcggaaaaa
     1921 gaacaagccg tccggcacga aaccatacgg caagaccaaa ccttcaacag gcgcgggcaa
     1981 ctgtacggct tcatcagcgt catcctgata ctgctttttg ccgtcttcct cgtatggagc
     2041 ggctaccccg caaccgccgc ctcccttgcc ggcggcacag tggttgcctt ggcgggtgct
     2101 ttcgtgattg gaagaagccg agaccaaggc aaaaattaat gacaaatcct agggcgtgct
     2161 tcatatccgc ccgaacgccg aaccgcacat ataggcacat cccgcgcgcc gcccggaagc
     2221 ggaagccgcg ccctcccaaa caaacccgaa tcccgtcaga taaggaaaaa taatgaaaac
     2281 aaccgacaaa cggacaaccg aaacacaccg caaagccccg aaaaccggcc gcatccgctt
     2341 ctcgcctgct tacttagcca tatgcctgtc gttcggcatt cttccccaag cttgggcggg
     2401 acacacttat ttcggcatca actaccaata ctatcgcgac tttgccgaaa ataaaggcaa
     2461 gtttgcagtc ggggcgaaag atattgaggt ttacaacaaa aaaggggagt tggtcggcaa
     2521 atcaatgaca aaagccccga tgattgattt ttctgtggtg tcgcgtaacg gcgtggcggc
     2581 attggtgggc gatcaatata ttgtgagcgt ggcacataac ggcggctata acaacgttga
     2641 ttttggtgcg gaaggaagca atcccgatca acatcgtttt acttataaaa ttgtgaaacg
     2701 gaataattat aaagcaggga ctaacggcca tccttatggc ggcgattatc atatgccgcg
     2761 tttacataaa tttgtaaccg atgcagaacc tgttgaaatg accagttata tggatgggcg
     2821 gaaatatatc gatcaaaata attaccctga ccgtgttcgt attggggcag gcaggcaata
     2881 ttggcgatct gatgaagatg agcccaataa ccgcgaaagt tcatatcata ttgcaagtgc
     2941 gtattcttgg ctcgttggtg gcaatacctt tgcacaaaat ggatcaggtg gtggcacagt
     3001 caacttaggt agtgaaaaaa ttaaacatag cccatatggt tttttaccaa caggaggctc
     3061 atttggcgac agtggctcac caatgtttat ctatgatgcc caaaagcaaa aatggttaat
     3121 taatggggta ttgcaaacgg gcaaccccta tataggaaaa agcaatggct tccagctggt
     3181 tcgtaaagat tggttctatg atgaaatctt tgctggagat acccattcag tattctacga
     3241 accacgtcaa aatgggaaat actcttttca cgacaataat aatggcacag gaaaaatcga
     3301 cgccaaacat gaacacaatt ctctgcctaa tagattaaaa acacgaaccg ttcaattgtt
     3361 taatgtttct ttatccgaga cagcacaaga acctgtttat catgctgcag gtggtgtcaa
     3421 cagttatcga cccagactga ataatggaga aaatatttcc tttattgacg aaggaaaagg
     3481 cgaattgata cttaccagta acatcaacca aggtgctgga ggattatatt tccaaggaga
     3541 ttttacggtc tcgcctgaaa acaacgaaac gtggcaaggc gcgggcgttc atatcagtga
     3601 agacagtacc gttacttgga aagtcaatgg cgtggcaaac gaccgcctgt ccaaaatcgg
     3661 caaaggcacg ctgcacgttc aagccaaagg ggaaaaccaa ggctcgatca gcgtgggcga
     3721 cggtaaagtt attttagatc aacaagcaga tgaaaataat aaaaaacaag cctttagtga
     3781 aatcggcttg gtcagcggca ggggtacggt gcaactgaat gccgataatc agttcaaccc
     3841 cgacaaactc tatttcggct ttcgcggcgg acgtttggat ttgaacgggc attcactttc
     3901 gttccaccgt attcaaaata ccgatgaagg ggcgatgatt gtcaaccaca atcaagacaa
     3961 agaatccacc gttaccatta caggcaataa agatattgct acaaccggca ataacaacag
     4021 cttggatagc aaaaaagaaa ttgcctacaa cggttggttt ggcgagaaag atacgaccaa
     4081 aacgaacggg cggctcaacc ttgtttacca gcccgctgca gaagaccgca ccctgctgct
     4141 ttccggcgga acaaatttaa acggcaacat cacgcaaaca aacggcaaac tgtttttcag
     4201 cggcagacca acaccgcacg cctacaatca tttaaacgac cattggtcgc aaaaagaggg
     4261 cattcctcgc ggggaaatcg tgtgggacaa cgactggatc aaccgcacat ttaaagcgga
     4321 aaacttccaa attaaaggcg gacaggcggt ggtttcccgc aatgttgcca aagtgaaagg
     4381 cgattggcat ttgagcaatc acgcccaagc agtttttggt gtcgcaccgc atcaaagcca
     4441 cacaatctgt acacgttcgg actggacggg tctgacaaat tgtgtcgaaa aaaccattac
     4501 cgacgataaa gtgattgctt cattgaccaa taccgacatc agcggcaatg tcagccttgc
     4561 cgatcacgct catttaaatc tcacagggct tgccacactc aacggcaatc ttagtgcaaa
     4621 tggcgataca cgttatacag tcagccacaa cgccacccaa aacggcgacc ttagcctcgt
     4681 gggcaatgcc caagcaacat ttaatcaagc cacattaaac ggcaacacat cggcttcggg
     4741 caatgcttca tttaatctaa gcgaccacgc cgtacaaaac ggcagtctga cgctttccgg
     4801 caacgctaag gcaaacgtaa gccattccgc actcaacggt aatgtctccc tagccgataa
     4861 ggcagtattc cattttgaaa gcagccgctt taccggacaa atcagcggca gcaaggatac
     4921 ggcattacac ttaaaagaca gcgaatggac gctgccgtca ggcacggaat taggcaattt
     4981 aaaccttgac aacgccacca ttacactcaa ttccgcctat cgccacgatg cggcaggggc
     5041 gcaaaccggc agtgcgacag atgcgccgcg ccgccgttcg cgccgttccc tattatccgt
     5101 tacacctccg gcttcggtag aatcccgttt caacacgctg acggtaaacg gcaaattgaa
     5161 cggtcaggga acattccgct ttatgtcgga actcttcggc taccgcagcg acaaattgaa
     5221 gctggcggaa agttccgaag gcacttacac cttggcggtc aacaataccg gcaacgaacc
     5281 tgcaagcctc gaacaattga cggtagtgga aggaaaagac aacaaaccgc tgtccgaaaa
     5341 ccttaatttc accctgcaaa acgaacacgt cgatgccggc gcgtggcgtt accaactcat
     5401 ccgcaaagac ggcgagttcc gcctgcataa tccggtcaaa gaacaagagc tttccgacaa
     5461 actcggcaag gcagaagcca aaaaacaggc ggaaaaagac aacgcgcaaa gccttgacgc
     5521 gctgattgcg gccgggcgcg atgccgtcga aaagacagaa agcgttgccg aaccggcccg
     5581 gcaggcaggc ggggaaaatg tcggcattat gcaggcggag gaagagaaaa aacgggtgca
     5641 ggcggataaa gacaccgcct tggcgaaaca gcgcgaagcg gaaacccggc cggctaccac
     5701 cgccttcccc cgcgcccgcc gcgcccgccg ggatttgccg caactgcaac cccaaccgca
     5761 gccccaaccg cagcgcgacc tgatcagccg ttatgccaat agcggtttga gtgaattttc
     5821 cgccacgctc aacagcgttt tcgccgtaca ggacgaatta gaccgcgtat ttgccgaaga
     5881 ccgccgcaac gccgtttgga caagcggcat ccgggacacc aaacactacc gttcgcaaga
     5941 tttccgcgcc taccgccaac aaaccgacct gcgccaaatc ggtatgcaga aaaacctcgg
     6001 cagcgggcgc gtcggcatcc tgttttcgca caaccggacc gaaaacacct tcgacgacgg
     6061 catcggcaac tcggcacggc ttgcccacgg cgccgttttc gggcaatacg gcatcgacag
     6121 gttctacatc ggcatcagcg cgggcgcggg ttttagcagc ggcagtcttt cagacggcat
     6181 cggaagcaaa atccgccgcc gcgtgctgca ttacggcatt caggcacgat accgcgccgg
     6241 tttcggcgga ttcggcatcg aaccgcacat cggcgcaacg cgctatttcg tccaaaaagc
     6301 ggattaccgc tacgaaaacg tcaatatcgc cacccccggc cttgcgttca accgctaccg
     6361 cgcgggcatt aaggcagatt attcattcaa accggcgcaa cacatttcca tcacgcctta
     6421 tttgagcctg tcctataccg atgccgcttc gggcaaagtc cgaacgcgcg tcaataccgc
     6481 cgtattggct caggatttcg gcaaaacccg cagtgcggaa tggggcgtaa acgccgaaat
     6541 caaaggtttc acgctgtccc tccacgctgc cgccgccaaa ggcccgcaac tggaagcgca
     6601 acacagcgcg ggcatcaaat taggctaccg ctggtaacac cggatatgcc gaaaggggtt
     6661 tgacgatgcc gccgtgcgct gtcaaaccct ttttctgccg ccgccttgtg cctgctccat
     6721 tgtctgataa ccgtcaaatt gatttttagc ccatgtttgg tgggtcgggg aaatctatat
     6781 cttcgtgcgt gccgaaatag tctatgcccg atatacaatt ttgatacaca aacttggaaa
     6841 tatcggtatc gtcgccggag cgatagaatg cggacagttt ttcattgaat tgcggcatct
     6901 gcatttcgga gatttccaag atgccgcagc cccccgccat catcagaccg ttggcaaata
     6961 aggttgccgt tcgtttgttg ccgtcccaaa aaacctgctg ccgcatacaa taaagcataa
     7021 aacggacggc tgcctccgtc gtcgaaccgc tttgcagtcc gatattttgt aacacgcggg
     7081 ccacttcaat ttccttcact ggattcgggg catgacggga accgtccaat agcgttacgc
     7141 cgaccgaacc ggtacggaaa tcaccgggtg ccaaagaatc gtccttggca acaatgttgt
     7201 tgattttttt aaggagtgaa atattgaccg gttcgccatt tgaaatatgc gaaatcacat
     7261 attgataggc acgtttcagg ttcaagatgg tttggatgtc ttcaagtgac acggaggcta
     7321 catttttgcc atggataatt tgttcggtct gcaataaggt cgtctgacaa ttctcaaaac
     7381 ggctgaggtt gtgaatttgg gcaaccaata ctttctttgc caaaaaaata ttttctttca
     7441 aactcaactt gtatttgtcg ggaaacacga taattcctta aataattaaa gcagtacgtt
     7501 aaatcatcat cttatcatca ctttcggggt ttatgttgca tgcgtccgtt ttctaatacg
     7561 gtttcccatc ccgcgaaacg aggcggaggc ctgtgcaaaa aacctgttca cgaaattttc
     7621 cgcattaacg atacgctgaa aatgcgctaa aaatgactgt gtttgaatat cggttgattt
     7681 catccgtttg tgtaacctcg cgccggtttt gtcatgattt tgcattatag tg
//
LOCUS       DAWEWP010000091         7726 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWEWP010000091 DAWEWP010000000
VERSION     DAWEWP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.091"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(69..485)
                     /gene="dksA"
                     /locus_tag="WGN53_001399"
     CDS             complement(69..485)
                     /gene="dksA"
                     /locus_tag="WGN53_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ0140383.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(632..1423)
                     /locus_tag="WGN53_001400"
     CDS             complement(632..1423)
                     /locus_tag="WGN53_001400"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ0140384.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1472..1879)
                     /locus_tag="WGN53_001401"
     CDS             complement(1472..1879)
                     /locus_tag="WGN53_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ0140385.1"
                     /translation="MEIRAIKYTATAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRAKEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1879..2565)
                     /locus_tag="WGN53_001402"
     CDS             complement(1879..2565)
                     /locus_tag="WGN53_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ0140386.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2685..3728
                     /locus_tag="WGN53_001403"
     CDS             2685..3728
                     /locus_tag="WGN53_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ0140387.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3891..5117
                     /locus_tag="WGN53_001404"
     CDS             3891..5117
                     /locus_tag="WGN53_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0140388.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5403..7553
                     /gene="yccS"
                     /locus_tag="WGN53_001405"
     CDS             5403..7553
                     /gene="yccS"
                     /locus_tag="WGN53_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ0140389.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQENVANAYEALGSYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETSSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLSETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1901 a         2566 c         1692 g         1567 t
ORIGIN      
        1 gactggtgcg cctttgtgaa tatactgtct gaaacttagg gcttcagacg gcattttgtt
       61 tgccgccatc agtcggcaaa ctgttttttc atcctctctc ggcgttcttg ggactcaaca
      121 gataaagtgg ctgtcgggcg tgccagcagc cgcttcagac cgataggctc tcccgtatcg
      181 gcacagaatc cataatcccc ttcatcaata ttgcggatgg tcgcctgtat tttactgaga
      241 agttttcgtt cccgatcgcg ggtacggagt tccaatgcgt actcttcttc ctgtgtggca
      301 cggtcggcag gatcgggggc tgattcgtgt tcttggagat gccctgtcgt agcggaagca
      361 ttttcgatga gttcgtcttg catttttacc agcaattcgc ggaaaaaagc caaatggtcg
      421 tcattcatat aatcgtcttc cggcccgctc caattcaaaa tatcttgttc tgtcagcttt
      481 gccataatgt atatcctctc ggttcaccga aaaaaacaaa gcaccacaaa gtggctttat
      541 tcctttgatt tttataaggc ttattgccta aggcgttttt gaaaacgcgc atcataacac
      601 gttttggatt ggttttttta ttttctttac attattgata ttgccgttcc atttcctgcg
      661 aacggcgcac acaggcacaa acgccctcac ttatggcttc ggcgacacga tgccgcctga
      721 aggcttccac ggcttcgtgg gttgtcccgc cttttgacgt tacatttttt tgaagcttct
      781 cgaaatcttc acccgtctgc tcggcaaggg caaccgctcc tttaaacgtt gccagactga
      841 gcgcgcgtgc ttctgccata tcaaaccctt gtcggatggc ggcattttgc aatgcgtcca
      901 gcagataaaa cacatatgcc ggcccgctgc cgctgatacc ggtaatgctg tgcatttgcg
      961 cttcatcttc caaccaaaca gtcaaaccga ctgatttcat gattcgatcg gcaatcctgc
     1021 ggtctgtttc cgatacttcc gcttcggcat acataccgga tacgcccagc ccgatttttc
     1081 cgggtgtatt cggcataacc cggacaatgc ggcgtgttcc cccgaggtaa cggctgagcg
     1141 tatcgaccgg caatccggct gcgacagaaa gcaccaatgc gccgttggtg cggatatttt
     1201 tgcacgcggc ttccatatcc tgcggcttga cggcaaggat taaaacatcg tcggaatgaa
     1261 gctccggcaa ggtttcagaa gtttcgaccc ccaactcttt ttgcaaacgt tcgcgctttt
     1321 ccgcaccccg gttggcaata tggatgcggt aaccgccttg tttgaccaat ccgcccgcaa
     1381 cggcagccgc catattgccg ccgccgagaa aataaacatt cattatttct accctattca
     1441 aaattacctg actaaattca ttcccaattt tttaccatcg gacgcgctcg gacagaccgt
     1501 tgcgccgcaa cccctgcttt ttcaaacaat caaaaccttc ctgtttgaac gtctcaatca
     1561 aaggtttgcc gtcacgcgta cgccaatcca acccgcaggc ttcaaacttc cctattttcc
     1621 tgtaaaagta agattgttgt gcggattcat attcctttgc cctgacggaa cgattgccgt
     1681 ctgaaagcgg tatctcgtag tcctctaaag acggcggact ctcgccgcca atatcccaaa
     1741 aatcgatggc ggccggtttt ctcggcttac accagccgga cagggacgaa cactcatacc
     1801 aacccgccaa ccggcagcct gcaaccgtaa aggcaagcaa cgcagccgtt gccgtatatt
     1861 ttattgcccg aatttccatc atcccctttt cccgaaaatc gcgctgccga tacggacgtg
     1921 tgtcgcaccg cactcaatgg cggcaggcat atcgtccgac atccccatag acagcacgtc
     1981 cgccttaacg ccagccgtat tgaggtcggc aagcaacttt tgcatggtct gaaactgggt
     2041 tttcaactcc gtttcactgc tgttggcttt ggcaacgcac atcagtccgc gcacgacaag
     2101 gttgggcaac ttcgccactt cgaccgcaag cgcgaccgct tcttcgggag caacaccgtg
     2161 ctttgccgcc tcgcctgcaa tattcacctc gatacacacc tgcaaaggcg gcattgaaga
     2221 aggacgctgc ccactcagcc ggacggcggt ctttagacgg catacggtat gcacccaatg
     2281 cgcgcgttcg gcgacaaact tggttttgtt ggactgcaca tcgccgatga cgtgccacac
     2341 gatgtcggtc aaatccgcca actcttccgt tttgccgtac cactcctgaa tatagttctc
     2401 gccgaaatca cgctgtccgg cggcgtaaac ttcgcggatg ccgtctgaag ggaaagtctt
     2461 accgacggca atcaggctga cggaatgcgg cttccttccc gcctgcagaa ccaattttcc
     2521 gatacggtcg gacacctccc gataacgttc ttgcaacacc gtcatagatt atcccctaat
     2581 taaaaatgat taaacagttg aaacccctcc agtcagggcg gtacaatcaa ggttgttaga
     2641 accattccaa ccaatcgaaa cattatacta aacagagccg cattatgcag attaccgact
     2701 tactcgcctt cggcgctaaa aacaaagcat ccgaccttca cctgagttcg ggcatatccc
     2761 ctatgattcg ggttcacggc gatatgcggc gcatcaacct tcccgaaatg agcgcggaag
     2821 aggtcggtaa tatggtaact tcggtgatga acgaccacca gcggaaaatc taccagcaaa
     2881 acttggaagt cgacttctcg ttcgaactgc ccaacgtcgc ccgattccgc gtcaacgcct
     2941 tcaacaccgg ccgcggtccc gccgccgtat tccgcaccat tcccagcacc gtcttatcgc
     3001 tggaagaatt gaaagccccg agcattttcc aaaaaatcgc agaatcgccg cgcggcatgg
     3061 tattggttac cggccctacc ggttcgggca aatcgaccac gcttgccgcg atgatcaact
     3121 acatcaacga aacccagccg gcacacatcc tgaccatcga agacccgatc gaattcgtcc
     3181 accaaagcaa aaaatccctg attaaccagc gcgagctgca ccagcacacc ctcagcttcg
     3241 ccaacgcgct gcgttccgca ttgcgcgaag accccgacgt tatccttgtc ggcgagatgc
     3301 gcgaccccga aaccatcggc ttggcactga ccgccgccga aaccggacac ttggttttcg
     3361 gcacgctgca cacgaccggc gcagcaaaaa ccgtcgaccg tatcgtggac gtattcccgg
     3421 cgggagaaaa agaaatggtg cgctctatgc tgtccgaatc gctgaccgcc gtcatctccc
     3481 aaaacctgct gaaaacgcac gacggcaacg gccgtgtcgc ctcgcacgaa atcctgattg
     3541 ccaaccccgc cgtccgcaac ctcatccgcg aaaacaaaat cacgcagatt aactccgtcc
     3601 tgcaaaccgg gcaggcgagc ggtatgcaga caatggacca atcgctgcaa tcgctggtgc
     3661 gccaagggct gatcgcaccg gaagtcgcac gcagacgcgc gcaaaacagc gaaagtatga
     3721 gtttctgaca cacaaccgcc ttccggccat accggcggga aaacaaggcg caaacacgcg
     3781 gggcgggacg cagcatcccg cccggctacc tttccgaaca agaagcgtcc gccttcctgt
     3841 tgaaacctgc cgccgcaaac tgcaaggctt aaaccgaaaa gaagctaacg atgaataccg
     3901 ataacctgca cgacatcttg gacgaaatgg ttcaagtgta ttctcaaaaa aaacaaagcc
     3961 gatccgaaac cccggccgaa atcggcgcac acttccaccc gctgctcgac cgcctgtgcg
     4021 aaaccgcaga agcacaaaac gcgtccgaca tccttatcag caaaggattc ccgccctcgt
     4081 tgaaaatcaa cagcgcatta accccgcagc cgcaaaaggc gctgacgggc gaggaaaccg
     4141 ccgccatcgc cgcatcgacg atgaacgccg aacaatcgga aatattccgg cgcgacggcg
     4201 aaatcaacta ctccgtccag tcgcgcagcg gcacgcgcta ccgcgccaac gcctaccaca
     4261 gccaaggcag cgcaggtttg gttttgcggc gcatcaacca cgtcatcccg caaatgcagg
     4321 aattgggcct gcccgaaaaa ctcaaagacc tcgccgtcgc accgcgcggg ctgctgatta
     4381 tcgtcgggcc taccggttcg ggcaaatcca ccacgatggc gactatgctc gaacaccgca
     4441 acaaaaccct gcccagccat atcgttacca tcgaagaccc gattgagttt atctacaaac
     4501 cgcgccgctg catctttacc cagcgcgaaa tcggcgtcga caccataaac tggcagacgg
     4561 cggtacaaaa cgctatgcgc cagtcccccg acgtggtctg catcggcgaa gtccgcagca
     4621 gggaaagtat ggaatacgcg atgcagctcg cccaaaccgg ccacctgtgc atttttacgc
     4681 tccacgccaa caccgcgccg cagtcgctcg aacgcatact caacttctac cccaaagaac
     4741 agcacaacca aatactgatc gacatcgccc tcaacctgac cggcatcatc tgccaacgcc
     4801 tcgccctcaa acaagacaaa acgggcagga cggcggttgt cgacttgctc atcaacacgc
     4861 ccgccatcca agacttcatc ctgaagggcg acctgatgaa catcagtaaa atcatggaaa
     4921 ccgccaaaac cgacggaatg cagacgatgg atcaaaacct tttcgaactg taccgtcacg
     4981 gcatcatcag ttacgaagaa gccctgcgcc agtccgtttc cgccaacaac ctgcgattgc
     5041 acatccaact gcacaaagaa ggcaaaacgc ccgaactcct ttacgacagg gtcaacggtc
     5101 tcaacctcat ttcctgatcc gcaaaaccca atgccgtctg aaaaccgcat ccccgttttc
     5161 agacggcatg attttatccg ccccattcat gtgctacact ttatagtgga ttaaatttat
     5221 agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagcacaa atagtacgga
     5281 accgattcac ttggtgcttg agcaccttag agaatcgttc tctttcagct aaggcgaggc
     5341 aacgccgtac tggtttttgt taatccacta tattcacttc ataacaataa accggtaaaa
     5401 ccatgaaaac cccactcctc aagcctctgc tcattacctc gcttcccgtt ttcgccagtg
     5461 tttttaccgc cgcctccatc gtctggcagc taggcgaacc caagctcgcc atgcccttcg
     5521 tactcggcat catcgccggc ggccttgtcg atttggacaa ccgcctgacc ggacggctga
     5581 aaaacatcat cgccaccgtc gccctgttta ccctctcctc gctcacggca caaagcaccc
     5641 tcggcacagg gctgcccttc atcctcgcca tgaccctgat gaccttcggc ttcaccattt
     5701 taggcgcggt cgggctgaaa taccgcacct tcgccttcgg cgcactcgcc gtcgccacct
     5761 acaccacact tacctacacc cccgaaacct actggctgac caaccccttc atgattttat
     5821 gcggcaccgt actgtacagc accgccatca tcctgttcca aatcatcctg ccccaccgcc
     5881 ccgttcaaga aaacgtcgcc aacgcctacg aagcgctcgg cagctacctc gaagctaaag
     5941 ccgacttttt cgatcccgac gaagccgaat ggataggcaa ccgccacatc gacctcgcca
     6001 tgagcaacac cggcgtcatc accgccttca accaatgccg ttccgccctg ttttaccgcc
     6061 ttcgcggcaa acaccgccac ccgcgcaccg ccaaaatgct gcgttactac tttgccgccc
     6121 aagacataca cgaacgcatc agctccgccc acgtcgatta tcaggaaatg tccgaaaaat
     6181 tcaaaaacac cgacatcatc ttccgcatcc accgcctgct cgaaatgcag ggacaagcct
     6241 gccgcaacac cgcccaagcc ctgcgcgcaa gcaaagacta cgtttacagc aaacgcctcg
     6301 gccgcgccat cgaaggctgc cgccaatcgc tgcgcctcct ttcagacagc aacgacagtc
     6361 ccgacatccg ccacctgcgc cgccttctcg acaacctcgg cagcgtcgac cagcagttcc
     6421 gccaactcca gcacaacggc ctgcaggcag aaaacgaccg catgggcgac acccgcatcg
     6481 ccgccctcga aaccagcagc ctcaaaaaca cctggcaggc aatccgtccg cagctaaacc
     6541 tcgaatcagg cgtattccgc catgccgtcc gcctgtccct cgtcgttgcc gccgcctgca
     6601 ccatcgtcga agccctcaac ctcaacctcg gctactggat actactgacc gcccttttcg
     6661 tctgccaacc caactacacc gccaccaaaa gccgcgtgta ccaacgtatc gccggcaccg
     6721 tactcggcgt aatcgtcggc tcgctcgtcc cctacttcac cccctccgtc gaaaccaaac
     6781 tctggatcgt catcgccagt accaccctct ttttcatgac ccgcacctac aaatacagtt
     6841 tctccacctt cttcatcacc attcaagccc tgaccagcct ctccctcgca gggttggacg
     6901 tatacgccgc catgcccgtg cgcatcatcg acaccattat cggcgcatcc cttgcctggg
     6961 cggcggtcag ctacctatgg cccgactgga aatacctcac gctcgaacgc accgccgccc
     7021 ttgccgtatg cagcaacggc gcctatctcg aaaaaatcac cgaacgcctc aaaagcggcg
     7081 aaaccggcga cgacgtcgaa taccgcgcca cccgccgccg cgcccacgaa cacaccgccg
     7141 ccctcagcag caccctttcc gacatgagca gcgaacccgc aaaattcgcc gacagcctgc
     7201 aacccggctt taccctgctc aaaaccggct acgccctgac cggctacatc tccgccctcg
     7261 gcgcataccg cagcgaaatg cacgaagaat gcagccccga ctttaccgca cagttccacc
     7321 tcgccgccga acacaccgcc cacatcttcc aacacctgtc cgaaaccgaa cccgacgact
     7381 ttcagacagc actggataca ctgcgcggcg aactcgacac cctccgcacc cacagcagcg
     7441 gaacacaaag ccacatcctc ctccaacagc tccaactcat cgctcggcag ctcgaaccct
     7501 actaccgcgc ctaccgacaa attccgcaca ggcagcccca aaacgcagcc tgaaaaagtt
     7561 tcggcatttt gtaagagagg gcagattgtc agacaggttg caagatagtg gataaagctt
     7621 tgcgcagggt aaatgcgtag caactgaacc gtcattccca cgaacctaca tcccgtcatt
     7681 cccacgaaaa cagaaaacca aaaacagaaa cctaaaatcc cgtcat
//
LOCUS       DAWEWP010000092         7677 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWEWP010000092 DAWEWP010000000
VERSION     DAWEWP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.092"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(171..1472)
                     /locus_tag="WGN53_001406"
     CDS             complement(171..1472)
                     /locus_tag="WGN53_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ0140390.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPNLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDGPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(1462..2373)
                     /locus_tag="WGN53_001407"
     CDS             complement(1462..2373)
                     /locus_tag="WGN53_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0140391.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            2481..2879
                     /locus_tag="WGN53_001408"
     CDS             2481..2879
                     /locus_tag="WGN53_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0140392.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            2928..3533
                     /locus_tag="WGN53_001409"
     CDS             2928..3533
                     /locus_tag="WGN53_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0140393.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEIVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALCAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            3719..5830
                     /locus_tag="WGN53_001410"
     CDS             3719..5830
                     /locus_tag="WGN53_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0140394.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGYSRAFTASIDPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSINTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDEQNDPYTWAVGGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAMLGWNHKNVNITFAAANLLNQKY
                     WRSDAMPGAPRTYTARVNYSF"
     gene            6033..6692
                     /locus_tag="WGN53_001411"
     CDS             6033..6692
                     /locus_tag="WGN53_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ0140395.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            6863..7558
                     /locus_tag="WGN53_001412"
     CDS             6863..7558
                     /locus_tag="WGN53_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0140396.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         1895 a         1947 c         2053 g         1782 t
ORIGIN      
        1 ttcatcgact tctatttttg ccggcgatgg gaacggtgtc ggcttgggtg gcggtgattt
       61 gggcatacct gatgattgaa agtgaaaaaa acggaagata ttgagtcatt cggacgcaat
      121 gccgtctgaa acggaagttc agacggcatt tgttttaggt tccgtaccgc ttagggaata
      181 ccggcgacag gataggcggg atagccgtgg gtatcgaccg aacaggcaaa ccgccaaggc
      241 gtgtggacgg tgtcggcgga caggtgggcg agttcgggaa tgtgccgttt gataaaagta
      301 ccgtccgggt cggtacggta tgaggcggcg gcaatgtcgg ggcaggtgtg ccgtgaggcg
      361 gcaagccacc agttgccttg gttgattgcc gcatcgaaat cggtcagctg ccgggcaaac
      421 catatctcgc cttcgcggcg ggggaggttt aaaacgtggc agaaaaaatc cgcgctcaag
      481 cgtctcaggg cggggtggag gctgccggtt ttgtgcaaac agcgcatcgc ggcatcgata
      541 atcggaatgc cggtctggcc ctgctgccaa agcgtcaggc gcagggtgtg ttccggattg
      601 ccgtctgaag ggccgtcatc cgtgtgctgc aaggcaagtt gaaggaaaaa atcgcggcgg
      661 atgatgttgt ccgcccacgc gttcagacgg cgttcgaggc tttcccgcgc gagcaggcgc
      721 ggcgagatgc agccggcact caaatacgcg cccatcagcg aagtgtgttt gcgcgagggg
      781 aaatccttta aaacggagta ggaatccgcc tgttcgagaa accgccgcca ctgccgccaa
      841 gccgccgttt cgccgctgtt ttgcggcagg aagatgccgt ctgaaagggc ggcaggcggc
      901 ggggcggaaa ggttttcggg gaagggttgg cggtatgccg cgaataggtt cggaccggcg
      961 gggggctgct tggaaaagcg gtcgagccat acttcgcggt agcggtcgaa atcggtgtgt
     1021 gccgtgccgc cgtcgggtat caggtcggtt ttgccgaaaa cggcgcggtc attgacgaag
     1081 gttaacgcga tgccgtgttt gtccaattca tgccaaaggg cgttgtcggc gagtttgtcg
     1141 gcaaaagtat gggattcgtc ggcgatgacg gcgcggatat tgaggcggac ggcgagccgg
     1201 acgagctcgg caggcgatgc cgccgtgtag agcgggatgc cgcgccctgc aagcccttgg
     1261 gcgagttcgg cggcggattg gcggtagaac gcggcgcggc gagggttgtc tgtttcggca
     1321 tcgtcaatcc aaataccgat aacgggcaaa ccttcggcaa cggcggtgca taaggcggcg
     1381 ttgtcgcgga tgcggaggtt ttggcggaac cagacgagcg tgtgtgcggc gcacgtgtcc
     1441 gcataaaggg ggcgggcggt ttcagacggc atttcggcag cctttcctgc tggcgatttt
     1501 ttcgttcaga aaatcgatga agctgcggac tttcgcgctt aagaacgccc tgtccgcata
     1561 aacggcattc aggcggtcgg tcgggacggt atattcgggc agcagcctca ccagcgagcc
     1621 gcagcgcaaa tcgtgttccg ccgcccaaag cggctgataa ccgatgcacg cgcccgcctt
     1681 aatcatttcg cgcatcatca gcgtgttgtc ggtacggatg acgggggtca gttcaagccg
     1741 gtattttttg ccgtccgatt tgcgggtgag gtcgagtttc tgctggttgg tgtaggtcgg
     1801 caggacggcg ggcagccccg ccacttcttc cggcgtttcc ggtacgccgt tgcgcctcag
     1861 gaaatcgggc gaggcgagca gggcaaattc gatttccgcc agcgggcgcg caatcagcga
     1921 cggggacagg gtttgggaaa cgcgcaacgc caaatccacg ccttcggcaa tcaaatcgac
     1981 gtggcggttg tccaaaatca gttctaatgt cacttcggga taacgttcgc ggtattccgc
     2041 cagccagttg catatctggc tgccggcaaa ccacagtggc atcgttacgc gcagcagccc
     2101 ctgcggtttt tccgtccccc cggcggcttt ttgcgcggca tcgtcgagcg tgtcgagcgc
     2161 gtaactgcat tgccggtagt attcttcccc ggcttcggtc aggctgaggt tgcggctgtt
     2221 gcggtgcagg agtttggctt ggacggtgtt ttccaagtgg ctgacgtgtt tgcttgccat
     2281 tgcggtggag atgccgagcg cgtcggcggc gcgggtgaag ccgccgcttt ggacgacttg
     2341 gcggaaaacc ttgaggctga acagggtgtc catattttct tgtgtggaaa agttgtatca
     2401 ataaaagcag tatatatttg aaaaggggaa acatctatac tctaccgcct gaaatgaaga
     2461 caaatatcaa aggagctttt atgtccgatt gctgcaaccg tatccaaccg gttttgcttt
     2521 ctgttttgcg tatcgtaacc gcttacctgt ttttgttgca cggtacgtcg aaaatcttcg
     2581 ccttccccat tgaaatgggc agcggttcgc ccggcgggct gttgctgctt gccggtattt
     2641 tagaaattgt cggcggcatt ttgctggtgt tgggcctgtt tgcgcgccct gccgcgtttg
     2701 ttttgtccgg ccagatggcg gttgcctatt ttatggcgca cgcttccgga aatgctttgt
     2761 tcccgattgc caacggcggc gagtccgcag tgctgttctg cttcgtattc ctctatatcg
     2821 cggcggcggg cggcggagca tggtcgctgg acaggctgtt tttcaagcgt aaagcctgaa
     2881 tcagactgcc taaagtgtat tttgttgaat gtttttgagg aaaagaaatg acccgtcaat
     2941 ctctgcaaca ggctgccgaa agccgccgtt ccatttattc gttaaataaa aatctgcccg
     3001 tcggcaaaga tgaaatcgtc caaatcgtcg aacacgccgt tttgcacaca ccttcttcgt
     3061 tcaattccca atctgcccgt gtggtcgtgc tgtttggcga agagcatgat aaggtgtggc
     3121 aatttgtcga agacgcgctg tgtgccgtcg tgcctgccga cagttttgaa ccgaccgcgc
     3181 aaaaattgaa cctgtttaag gcgggtgcgg caactatttt gttttatgaa gatcaaaatg
     3241 tcgtcaaagg tttgcaggag cagttccctg cttatgccgc caactttccc gtttgggcgg
     3301 accaggcgaa cgcgatggtg cagtatgccg tttggacgac acttgccgcg gtcggcgtag
     3361 gtgcaaacct gcaacattac aatcccttgc ccgatgcggc gattgccaaa gcgtggaata
     3421 tccccgaaaa ctggttgttg cgcgcacaaa tggttatcgg cggtattgaa ggggcggcag
     3481 gtgaaaagac ctttgaacca gttgcagaac gtttgaaagt gttcggcgca taatttcgcg
     3541 gtcaaaaaaa tgccgtctga accctgttca gacggcattt ttcagtatca ggcggcgagt
     3601 tttccgcatt ctgagacctt tgtttacaaa tatcatgttc aatatagtta aaagaaatta
     3661 ttctcatttc ctccgtgagg caatataatt cggttgtttt gttaaattga gtataaaaat
     3721 gaaaatatca tttcatttag ctttattacc cacgctgatt attgcttcct tccctgttgc
     3781 tgccgccgat acgcaggaca atggtgaaca ttacaccgcc actctgccca ccgtttccgt
     3841 ggtcggacag tccgacacca gcgtactcaa aggctacatc aactacgacg aagccgccgt
     3901 tacccgcaac ggacagctca tcaaagaaac gccgcaaacc atcgatacgc tcaatatcca
     3961 gaaaaacaaa aattacggta cgaacgattt gagttccatc ctcgaaggca atgccggcat
     4021 cgacgctgcc tacgatatgc gcggcgaaag cattttcctg cgcggttttc aagccgacgc
     4081 atccgatatt taccgcgacg gcgtgcgcga aagcggacaa gtgcgccgca gtactgccaa
     4141 catcgagcgc gtggaaatcc tgaaaggccc gtcttccgtg ctttacggcc gcaccaacgg
     4201 cggcggcgtc atcaacatgg tcagcaaata cgccaacttc aaacaaagcc gcaacatcgg
     4261 tgcggtttac ggttcgtggg caaaccgcag cctgaatatg gacattaacg aagtgttgaa
     4321 caaaaacgtc gccatccgtc tcaccggcga agtcgggcgc gccaattcgt tccgcagcgg
     4381 catagacagc aaaaatgtca tggtttcgcc cagcattacc gtcaaactcg acaacggctt
     4441 gaagtggacg gggcaataca cctacgacaa tgtggagcgc acgcccgacc gcagtccgac
     4501 caagtccgtg tacgaccgct tcggactgcc ttaccgcatg gggttcgccc accggaacga
     4561 ttttgtcaaa gacaagctgc aagtttggcg ctccgacctt gaatacgcct tcaacgacaa
     4621 atggcgtgcc caatggcagc tcgcccaccg cacggcggcg caggattttg atcatttcta
     4681 tgcaggcagc gaaaatggca acttaatcaa acgtaactac gcctggcagc agaccgacaa
     4741 caaaaccctg tcgtccaatt tcacgctaaa cggcgactac accatcggcc gttttgaaaa
     4801 ccacttgacc gtaggcatgg attacagccg cgaacaccgc aacccgacct taggttacag
     4861 ccgcgccttt actgcttcca tcgatccata cgaccgagca agctggccgg cttcgggcag
     4921 attgcagcct atcctcaccc aaaaccgcca caaagccgac tcctacggca tctttgtgca
     4981 aaacatcttc tccgccacgc ccgatttgaa attcgtcctc ggcggtcgtt acgacaaata
     5041 cacctttaat tccgaaaaca aactcaccgg cagcagccgc caatacagcg gacactcgtt
     5101 cagccccaac atcggcgcag tgtggaacat caatcccgtc cacacacttt acgcctcgta
     5161 taataaaggc ttcgcgcctt atggcggacg cggcggctat ttgagcatca acacgtcgtc
     5221 ttccgccgtg ttcaacgccg accccgagta cacccgccaa tacgaaaccg gtgtgaaaag
     5281 cagttggctg gacgaccgcc tcagcactac gttgtctgcc taccaaatcg aacgcttcaa
     5341 tatccgctac cgccccgacg agcaaaatga tccctacact tgggcagtcg gcggcaaaca
     5401 ccgttcgcgc ggcgtggaat tgtccgccat cgggcaaatc atccccaaaa aactctatct
     5461 gcgcggttcg ttgggcgtga tgcaggcgaa agtcgttgaa gacaaagaaa atcccgaccg
     5521 agtgggcatc catttgaata acaccagcaa cgttaccggc aacctgtttt tccgttatac
     5581 cccgaccgaa aacctctacg gcgaaatcgg cgtaaccggt acaggcaaac gctacggtta
     5641 caactcaaga aataaagaag tgactacgct tccaggcttt gcccgagttg atgccatgct
     5701 tggctggaac cataaaaatg ttaacattac ctttgccgca gccaatctgc tcaatcaaaa
     5761 atattggcgt tcggatgcca tgcccggcgc gccgcgcact tatacggcgc gggttaatta
     5821 cagtttctaa tcccgttcgt gcaacagaat gccgtctgaa aatcctgata atccgtaaat
     5881 tcagcacacg aactttttga caggctttat ctacatcagt ataatccaaa aagaaatagc
     5941 gcccttggca gtacgcatta cccattaaat catagcgggt atgccgatcc caagggcttt
     6001 tttatatctt tgacaaatat aggggaagca atatgaaaac cgccattttg attgacggtg
     6061 cattttttat taagcgggta cggtcattag gcctgtctca aaatccacac gatgcaaaaa
     6121 gaattgctga tttggctttc cgatattcga tgctgcatct gaaagataaa atcaacggca
     6181 cagatcaaat ggatgattta tatagaattt tcttttacga ctgcgtgccg tttgaaaaga
     6241 aaatgcacaa tcccgtatct catcgtgcca tagatttttc aaagactccg gaagccatat
     6301 ttcgttgcaa tctgcatacc gaattgaaga agaagcgtaa attagcgtta cgtttaggca
     6361 agctgtcgga caatacagca tggatattaa aaccccaagt catgaaaaat cttctgaaaa
     6421 acccgtcaac tcaaattacg gaaaacgatg tcgtgctcga tgttaaacaa aaaggtgtag
     6481 atatgcgtat aggcttggat atttcatcta ttaccttaaa aaaacaagcc gataaaatca
     6541 tcttgttttc tggtgattcc gattttgtcc cagcagccaa attagccaga cgggaaggta
     6601 tcgattttat tcttgatcct atgtggaatc atgtcccacc tgacttaatg gaacacatcg
     6661 acggattgaa gacaaaatgt ccgaaaccgt aaattactag aacaaaggcc gtctgaaaat
     6721 aaggctaatt atctaagcca tatttcagac ggcctttata aaggctgaaa taaaactaaa
     6781 cacatttttt cactcaaatc gaacacgcct tcaataaaat gccataaaat ccgcacatta
     6841 atctgacaca caagagatac ctatgaaact gaaaacctta gctttgactt cattgaccct
     6901 gttggcattg gccgcttgta gcaaacaggc tgaaaccagt gttccggcag acagcgccca
     6961 aagcagctca tctgctccgg cagcccctgc tgagttgaac gaaggtgtga actacactgt
     7021 attgtctacg cctattccgc aacagcaggc cggtaaaatc gaagtattgg aatttttcgg
     7081 ctacttctgc ccgcattgcg cccatcttga gccggtcttg agcgagcaca tcaaaacgtt
     7141 taaagacgat acctatatgc gccgggagca tgtcgtgtgg ggtgatgaaa tgaaaccttt
     7201 ggcacgtttg gcggccgcag tggaaatggc cggtgaatca gataaagcca acagccatat
     7261 tttcgatgcg atggttaatc aaaaaatcaa tctggccgat accgataccc tgaaaaaatg
     7321 gctgtccgag caaacagcgt ttgacggcaa aaaagtattg gctgcatttg aggctcctga
     7381 aagccaagcg cgtgcggctc aaatggaaga gttgaccaat aaattccaaa tcagcggcac
     7441 accgactgtg attgtcggcg gcaaatacca agttgaattt aaagactggc agtctggtat
     7501 gaccacgatt gaccagttgg tggataaagt acgcgaagag cagaaaaagc cgcaataagt
     7561 tgaggattga atgagtaaag gccatctgaa aataggattt cagacggcct tttgtattta
     7621 ggctttatag aagagatgat tgcttaaagc cttatggttt taaatcagaa tatatag
//
LOCUS       DAWEWP010000093         7670 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWEWP010000093 DAWEWP010000000
VERSION     DAWEWP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.093"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(308..730)
                     /locus_tag="WGN53_001413"
     CDS             complement(308..730)
                     /locus_tag="WGN53_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ0140397.1"
                     /translation="MQVTSKWIDGMCFVGTTESGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(848..1519)
                     /locus_tag="WGN53_001414"
     CDS             complement(848..1519)
                     /locus_tag="WGN53_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ0140398.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1535..2161)
                     /locus_tag="WGN53_001415"
     CDS             complement(1535..2161)
                     /locus_tag="WGN53_001415"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ0140399.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLHHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGVVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2268..3074)
                     /locus_tag="WGN53_001416"
     CDS             complement(2268..3074)
                     /locus_tag="WGN53_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0140400.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAASEPAAASAAQGDTSSIG
                     STMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMM
                     KFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQP
                     SKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYI
                     PSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3333..3773)
                     /locus_tag="WGN53_001417"
     CDS             complement(3333..3773)
                     /locus_tag="WGN53_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ0140401.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3836..5242)
                     /locus_tag="WGN53_001418"
     CDS             complement(3836..5242)
                     /locus_tag="WGN53_001418"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ0140402.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5412..6527
                     /gene="lptF"
                     /locus_tag="WGN53_001419"
     CDS             5412..6527
                     /gene="lptF"
                     /locus_tag="WGN53_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ0140403.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6524..7594
                     /gene="lptG"
                     /locus_tag="WGN53_001420"
     CDS             6524..7594
                     /gene="lptG"
                     /locus_tag="WGN53_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ0140404.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRFFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
BASE COUNT         1663 a         2097 c         2021 g         1889 t
ORIGIN      
        1 aataaattaa tatatggctt aaaataacgg gatgcccgcc ttccgcccgc ccgcagaagc
       61 aggcgggtat cattttaaaa cgcggcattt aaaatttgac cgaaaattgt tgacaatccg
      121 gaatcaagtc tgcacaatac cccgacaagt ccaagtatta taaaggctga ataaagagga
      181 aacagcaggc agatatattc gggaggtgca gtccgaatat atctgctttt ttatgcgcct
      241 ccggattgcc tgccgcacct ttcccttcag acggcatcag ccgtttcccc ataatgccgc
      301 ccgatgccta tttatctgcc ccggcaattt caaaactgtg ggtaatcttt gccgctttgc
      361 ccaacataat cgaagccgaa cagtattttt cggcagacat ctgaacggcg cgctcaatgg
      421 ccgattcttt caaatcatgc ccgaatactt tgaaatggat gtggatttcg gtaaacacgc
      481 gcggcgcatc gtccgcccgt ttcgccgtaa ccgtcgcacg gcagtcagtc actttctgac
      541 gctgtttttc ggcaatcatc accacatcga tgctcgaaca gcccgccacg cccaacagca
      601 gcatttccaa agggctgggc ccgcgcttgg ccgcaccttc tgccgccgaa ccctccataa
      661 cgacgctgtg cccgctttcc gtcgtgccga caaagcacat tccgtctatc cattttgatg
      721 taacctgcat ggtgtcattc ctgaaaatag cgttaaaacc gctttgcata tggcgttatt
      781 gtaaacaatt tcaagcggct tatgcagaaa tatggacaaa acggcaaaaa aacgcctgaa
      841 aaccgattta cggtttggcg gactggccat tgatctgcac cgatttgagt ttcagcgtat
      901 aggttttgcc gtcgtcggta tagccgattt gtgccggaat attgttcagg gacggtgcga
      961 agaaatacat taccgtatcg tcgccgcgcc gcacccgata tttgacgact tcggtttcca
     1021 cgccgcctat gctgtatttt cctgtacccg ccttattcaa accgccgacg gaataaagtt
     1081 ttttgccgtt ggtgattttc agccccgggg ggagtttcgc gtcatttgcc gccaactgcc
     1141 aggcaagcgt gaacaaatcc atagccttgg ggctttgctc ggttttgctc tcgcccgctt
     1201 tgccgtaggt tacgctgccg tcggcgaatt tggcttccgc atacagtttg cccctgcgta
     1261 tgtctctata gtaggtaggg tgcagggtat tgccgacaac cgtaccgccg gactcgaaac
     1321 ggatattgta tagcggcact ttaatcgtcg aaacgatttt gtaagcattg ccgctgcgtt
     1381 caaatgtcat cgtggcggga atgccgtagc tgccggaata gtgcagcacg gcggattggg
     1441 gcagccctgc cgcatacgcg cacggcaggg cggcggacaa aatggcggcg gaaaatatat
     1501 ttttaaaagt cttcatcatt tgctcccgcc cggtttacgc cgtcagaaaa cgggcggcat
     1561 cggcgttttc cgaatttctg acgcggtttc cctcgataat caggcggccg gcggcaaaat
     1621 cggcaacggc tttcggatag agtttatgct cgacagccaa aacccgggcg gcaacatcgt
     1681 ctgccgtatc gccgtcgagt atcggcacaa ccccttgcga tacaatcggg ccgcaatcca
     1741 gttcggcagt aacgaaatgg atggtgcagc cggcaacgcg acaacctgcc tccaaagcgc
     1801 gttcgtgcgt atgaagtccg gtaaacgagg gaaggatgga cgggtgaatg ttcatcagcc
     1861 tgccttcgta atgggcacag aactcagggg tcagaatccg cataaaacct gccaaaacca
     1921 ccaagtcggg ttgatatgcg tcgatttttt ccatcatggc ggtatcgaag gcaagtcgtg
     1981 attcaaagtt tttatgatgc aggctgtcgg tcgggatgcc gcgttcggcc gcccattgca
     2041 aaccggcagc cgtttcgctg ttgctcaaca cggcggcaat gcggacgttg ggaatggcgg
     2101 cattgacgat tgcctgcata ttgctgccgc gtccggaaat caggatgacg atgtttttca
     2161 taatggtacg cttttgaaag ggatgccgtc tgaaccgctg tttggtggtt tcagacggca
     2221 tttgccgtaa aaatgcccga aaacctgttt cgggcatgga ttcggactta atttactttt
     2281 ttgatgtcga cttgagccgg ctgcttggcg ggcgcgtttt cgggtgcgcc gattttgacc
     2341 agtttcacat caaataccaa agtggcgttc gggccgattt tgtcgcccgc accctgttcg
     2401 cggtaggcaa ggttggacgg gatgtagaac gtggcttcgc cgccttcttt cagaagctgt
     2461 acgccttcgg tccaacccgg aatcacttgg ctcaaaggga aggtgaccgg gccgccgttg
     2521 gctttgctgc tgtcgaatac cgtaccgtca atcaggcggc cttcgtattc cacggtaacg
     2581 atgtcgtctt tggacggctg tttgccttca ccctgtttgg tgattttgta ttgcaggccg
     2641 gaagcagtgg tcttcacgcc gtctttggcg gcattttctt tcagaaaggc ttcgcctttt
     2701 tctttattgg ccttcgcgtc cgccttgtgt ttttctacgg ctttagcctg ttgttcctga
     2761 aggaatttca tcatgacttc ctgagcctgc tcttcagtca ttttgatttc tttgccgtca
     2821 tacactgcct gcatggcttc ggtaaagact ttcaaatcga tttccgcacc ctgttccttc
     2881 atttgcttca gggagcgtcc gatgtccacg cccatcgcat agcttgcctg ctgcatcgtg
     2941 ctgccgatcg aagaggtgtc gccctgcgcg gcagaagcgg cggcaggttc ggatgcggct
     3001 tcttttttgc cacaggcgga aagtgccaaa gcggcggaaa gggtcagtgc gctgattttg
     3061 aaaatggtgt tcatgatgga tcttcgctgt cgataaggtc ggaaaaacgg gattatagcc
     3121 gagtttgaat gtttcaacac acaggatgac acataaagcg tcaatcgtgt gttgccctgt
     3181 tttggaaggg attgaacctt ccaaaataag ttttgattct accgccccga gggacagatg
     3241 tccaagtggc ggggttcaac cgataaggaa attttaatca aatagaatca agcctgttta
     3301 aattttgtaa atgcggcatt tcagacggca ttttatgcct tgccctccat gccgtgatgt
     3361 tcgatggcaa aaccgcttcg gcggtaggcg gtaaagcgtt cgcgcgcgtc ggcgagatct
     3421 tccaagctgt tgccgacgat ttccaaaacg cgcgcgggca agaccggagc ggtgttccaa
     3481 aaaccgtcgg acaggttcaa aacagtcatg ccttcgggaa ttcggggcgg attgccgccg
     3541 caagcaagca ggacggatgt ttcagacggc atggcttctt ccatttccca aatttcgtgc
     3601 ggaatgaaac tctcggcttc gtattgccaa agcattttgt ccaattcctg aagccgcccg
     3661 aacgaatcgg accacaccag tatcctgccg ccgtcccgaa tcgcacgggc aatcaggcgg
     3721 caggtgaaaa tcggaacttg ggcaacgtgc gtgtaaaagg tggctttcgg catattgttt
     3781 gagcatttgg caggacgatg ccgtctgaaa ggcttcagac ggcattgtgg caaaattaaa
     3841 gattccgcag atagttcagc agcaagggaa cgggacggcc agtcgcacct ttttccgctc
     3901 cggatttcca cgccgtaccc gcgatgtcaa ggtgcgccca cgggtagcct tcggtgaagt
     3961 aagacaggaa tgttgcggcg gtaatcgtgc ccgcgccggg cgtgccgata ttggggatgt
     4021 cggcaaagtt ggatttgagc tggtctttgt aggtttcaaa gagcggcagt tgccacgctt
     4081 tgtcgtccac gttgtaggaa gcggcaagca ggctgtcgac caaatcttga ttgttgccca
     4141 tcacgccgct gacatcgtgc cccaaggcga caatacacgc gccggtcagg gtggcgacgt
     4201 cgatgacggc tttgggtttg aactgttcgg cgtaagtgag cgcgtcgcac aaaatcagac
     4261 ggccttcggc atcggtgttc aacacttcga tggtcagacc tttcatactt ttcacgacat
     4321 cgcccggttt gtttgccgcg ccggaaggca tattttcaca agtggcgacg acggcaatca
     4381 ggttaatcgg cagttgcagt ttgacggcgg cgcagaaggt gctgatgacg gttgccgcgc
     4441 cgcacatatc aaacttcatt tcgtccatgt tcaggccggg tttgagggaa atgccgccgg
     4501 tgtcgaaggt aatgcctttg ccgaccaata ccacaggcgc ggcttctttg tccgccgcac
     4561 cgaaatagct cagttcgacc aaatagggat cttcgacact gcctttggcg accgaccaaa
     4621 acgaacccat gttttctttg atgtagtctt tttcgatgat tttggcgtgc gcgcccagtt
     4681 tttcggcttc ggctttggcg gtgcgcgcta aaaattcggg cgtgcattcg ttgggcgcgg
     4741 cgttgcccaa gtcgcggcag aggctttgtc cgtaaacttg cgcttcggcg acgcgcaagg
     4801 cttctttaac gacggcttcg tgtgcgctgt ggaacacggc ggtttcaaat ttggcgggct
     4861 tggcttcttt tttgtagcgg tcgaaacggt aggcggcgtt gccgaacgca atcgcaaacg
     4921 cttcggctac ggcggcggct tgcgcttcat cgaaggcatg gatgtccaca ttgaccgttt
     4981 cctgattttg cgcccatttg gcggcttcgg cggcggcctt gttcaatgcg gcgcggccgg
     5041 tgcttttcag acagcatacg gcaacagcct gcaaaccgtt gcctgtcggg atttttgtgt
     5101 cggcaaaatt ttgaccttct tcaagcgaag acaaaagggc aagggcggtc ggatggttta
     5161 attgcgcctc ttcggtgcag acaaataact gtgcgcctgc ctgctgttcc tgcaagattt
     5221 cggtttttgt gctaaattcc acgtttattc tcctgattga gacggttgtc ggtagttttc
     5281 ggacggcctt tcgctcaaaa gaccgtctga agacggctgg cacgattgta ccccatttga
     5341 agcaccgtct gaaaccttgc gcggacaatc cgcctgcgcc gaaccgctta ccgccccctg
     5401 accgcgattc tatgatttat caaagaaacc tcatcaaaga actctctttt accgccgtcg
     5461 gcattttcgt cgtcctcttg gcggtattgg tctccacgca ggcaatcaac ctgctcggcc
     5521 gtgccgccga cgggcgtgtc gccatcgatg ccgtgttggc attggtcggc ttctgggtca
     5581 tcggtatgac gccgcttttg ctggtgttga ccgcatttat cagtacgttg accgtgttga
     5641 cccgctactg gcgcgacagc gaaatgtcgg tctggctatc ctgcggattg gcattgaaac
     5701 aatggatacg cccggtgatg cagtttgccg tgccgtttgc cgttttggtt gccgtcatgc
     5761 agctttgggt gataccgtgg gcagagctac gcagccgcga atacgctgaa atcctgaagc
     5821 agaagcagga attgtctttg gtggaggcag gcgagttcaa cagtttgggc aagcgcaacg
     5881 gcagggttta ttttgtcgaa accttcgata ccgaatccgg catcatgaaa aacctgttcc
     5941 tgcgcgaaca ggacaaaaac ggcggcgaca acatcatctt cgccaaagaa ggtaacttct
     6001 cgctgaacga caacaaacgc acgctcgaat tgcgccacgg ctaccgttac agcggcacgc
     6061 ccggacgcgc cgactacaat caggtttcct tccaaaaact caacctgatt atcagcacca
     6121 cgcccaaact catcgacccc gtttcccacc gccgtaccat tccgaccgcc caactgattg
     6181 gcagcagcaa cccgcaacat caggcggaat tgatgtggcg catctcgctg accgtcagcg
     6241 tcctcctact ctgcctgctt gccgtgccgc tttcctattt caacccgcgc agcggacata
     6301 cctacaatat cttgattgcc atcggtttgt ttttaattta ccaaaacggg ctgaccctgc
     6361 tttttgaagc cgtggaagac ggcaaaatcc atttttggct cggactgctg cctatgcaca
     6421 tcatcatgtt cgtcatcgca atcgtacttc tgcgcgtccg cagcatgccc agccagccct
     6481 tctggcaggc ggttggcaaa agtctgacat tgaaaggcgg aaaatgaacc tgatttcacg
     6541 ttacatcatc cgtcaaatgg cggttatggc ggtttacgcg ctccttgcct tcctcgcttt
     6601 gtacagcttt tttgaaatcc tgtacgaaac cggcaacctc ggcaaaggca gttacggcat
     6661 atgggaaatg ctgggctaca ccgccctcaa aatgcccgcc cgcgcctacg aactgatgcc
     6721 cctcgccgtc cttatcggcg gactggtctc tctcagccag cttgccgccg gcagcgaact
     6781 gaccgtcatc aaagccagcg gcatgagcac caaaaagctg ctgttgattc tgtcgcagtt
     6841 cggttttatt tttgctattg ccaccgtcgc gctcggcgaa tgggttgcgc ccacactgag
     6901 ccaaaaagcc gaaaacatca aagccgccgc cattaacggc aaaatcagca ccggcaatac
     6961 cggcatttgg ctcaaagaaa aaaacagcat tatcaatgtg cgcgaaatgt tgcccgacca
     7021 taccctgctg ggcattaaaa tctgggcccg caacgataaa aacgaactgg cagaggcagt
     7081 ggaagccgat tccgccgttt tgaacagcga cggcagttgg cagttgaaaa acatccgccg
     7141 cagcacgctt ggcgaagaca aagtcgaggt ctctattgcg gctgaagaaa actggccgat
     7201 ttccgtcaaa cgcaacctga tggacgtatt gctcgtcaaa cccgaccaaa tgtccgtcgg
     7261 cgaactgacc acctacatcc gccacctcca aaacaacagc caaaacaccc gaatctacgc
     7321 catcgcatgg tggcgcaaat tggtttaccc cgccgcagcc tgggtgatgg cgctcgtcgc
     7381 ctttgccttt accccgcaaa ccacccgcca cggcaatatg ggcttaaaac tcttcggcgg
     7441 catctgtctc ggattgctgt tccaccttgc cggacggttc ttcgggttta ccagccaact
     7501 ctacggcatc ccgcccttcc tcgccggcgc actacctacc atagccttcg ccttgctcgc
     7561 tgtttggctg atacgcaaac aggaaaaacg ttgaaccaat gccgtctgcc ccccttcgga
     7621 cggcattggt ttttattatc atattcccgc agacagatag ccgttcccta
//
LOCUS       DAWEWP010000094         7659 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWEWP010000094 DAWEWP010000000
VERSION     DAWEWP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.094"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            191..1726
                     /locus_tag="WGN53_001421"
     CDS             191..1726
                     /locus_tag="WGN53_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0140405.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1723..1815
                     /locus_tag="WGN53_001422"
     CDS             1723..1815
                     /locus_tag="WGN53_001422"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ0140406.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="WGN53_001423"
     CDS             complement(1851..3095)
                     /gene="lysA"
                     /locus_tag="WGN53_001423"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ0140407.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDATKTIFSGVGKNEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTASVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3106..3276)
                     /locus_tag="WGN53_001424"
     CDS             complement(3106..3276)
                     /locus_tag="WGN53_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0140408.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDTARFGAIQTGLQL
                     QNKPQSAPQTRK"
     gene            3347..3670
                     /gene="cyaY"
                     /locus_tag="WGN53_001425"
     CDS             3347..3670
                     /gene="cyaY"
                     /locus_tag="WGN53_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ0140409.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3701..4120
                     /locus_tag="WGN53_001426"
     CDS             3701..4120
                     /locus_tag="WGN53_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ0140410.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCVNDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSREPT"
     gene            4142..4648
                     /gene="luxS"
                     /locus_tag="WGN53_001427"
     CDS             4142..4648
                     /gene="luxS"
                     /locus_tag="WGN53_001427"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ0140411.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVGNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            4794..7586
                     /gene="polA"
                     /locus_tag="WGN53_001428"
     CDS             4794..7586
                     /gene="polA"
                     /locus_tag="WGN53_001428"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ0140412.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGIESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDVFGSDSIGEQAALNAEMPSEKQAEKATAPERLDYQA
                     VTTEAQFAALLDKLSRANTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLGNPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAADQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVRNCLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
BASE COUNT         1767 a         2070 c         2083 g         1739 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtattcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc tgccggaatt
     1441 acgcaagcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct tcagacggca
     1861 ttttgatgtt cggcttgcag gcaggcgagt tcgtttgcca tttgctgttc caaggtttcg
     1921 cgccggcgga tgagtcggta ttcgttgccg tccaccaaca cctctgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat actggctccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tcgccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcgcaaatc
     2101 ggaccgacga tgttggcggt cagaggctca atgtctttgg tttcgaccgc ctcgatgtga
     2161 tgataggcat cataaagcgc cgggcgcatc aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgta tttgacgaat tcgacgcgtg tcagcagcga acctgcgttg
     2281 ccgaccaagc tgcggccggg ctcaagaatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaacggctt gggcatacgc gcccaaatca ggcacatttt cgtcttggta aacaatgccg
     2401 acgccgccgc ccaagtccaa gtgttccaac acaatgcctt cggcggcaag cgcgtcaacc
     2461 aaaatcaaaa tgcgctcgca ggcttcgacc agcgggctta agtcggtcag ttgcgaaccg
     2521 atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg gcggtaggct
     2581 tcgagcgcgt cggcgtaggc aatgccgaat ttgttggctt tcagacctgt ggagatgtag
     2641 ggatgggttt tcgcatcgac atcgggattg acgcgtaggg agacggacgc ggttttgccc
     2701 aaacgcgcgg caaccttttg aatacggtcg atttcgggaa tgctttccat attgaagcat
     2761 ttcacgcctg cattcagcgc gaactcgatt tccgcctcgt ttttgcccac gccggaaaaa
     2821 atcgttttcg tcgcgtcgcc gcctgccgcc aaaacgcgtg ccaattcgcc gccggacaca
     2881 atgtcaaaac cgctccccaa agaagcaaag tgtttgatga tgctcaggtt gccattggct
     2941 ttaaccgcat agcagacgag cggggagagc tcggcaaagg cagtttggta gtgttcaaat
     3001 gcttcggtca gcgcggattg gctgtacaca taaagcggtg tgccgaatgc ttcggcaagg
     3061 cgggggtagg ggacttgttc gcaaaacagg gtcatatctt cgttttcatt ttcgggtttg
     3121 tggggcggat tgcggtttgt tttgaagctg caaaccggtt tggattgcgc cgaaacgcgc
     3181 cgtatcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggcgg aaagcaggag
     3241 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
     3301 aatgtggcaa gattcggtat cttaaacaaa aaccacacaa aaagccatga tgaccgaaag
     3361 cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa tcgacgaaaa
     3421 cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag ccgaaaacgg
     3481 cacgcaaatc atcgtcaacc gccacacgcc caaccaagaa ttatggattg ccgccaaaag
     3541 cggtggctac catttcgccg aacaaaacgg caaatggctg gcaacgcgcg acggacggga
     3601 tttttacgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg tagagattga
     3661 ggaattgtga ttcaatttga tataaaaaga ttgttaccca atggcacaat tacctctata
     3721 tcggactgcc gaaatcggaa attttactgt cggcacgcct aaagttttag aatcattttc
     3781 caaacatatc ccttatggtg tcgtctttga agacgacggc gacacgggct acttctatgc
     3841 tgcttcgcaa gacgggattt tagatgcctt gcacatctac aatgtcgaag atgtatccga
     3901 caaacatatc cccaatcatg tcttgatttt atgggatgat gcctgcacca tagccgcatt
     3961 gtgtgtcaac gactacattc atgccgtcta tgattttgtc gaacaggcag gatattgccg
     4021 caacggcttc cctgaagcag gcggcgaatg ggtgaaagtc gaaaaccgcg tcttggatga
     4081 tgaattgctg gacaaaatcc tatcccgaga acctacataa ccctcacaaa aggataccca
     4141 aatgccccta ctagacagtt tcaaagtcga tcacacccgt atgcatgccc ccgccgtacg
     4201 cgtggcgaaa accatgacca cgcccaaagg cgacaccatt accgtgttcg acctgcgctt
     4261 ttgcattccc aacaaagaaa tcctgcctga aaaaggcata cacacgctgg agcatttgtt
     4321 cgcaggtttt atgcgcgacc acttgaacgg caacggcgtg gaaatcatcg acatttcccc
     4381 gatgggctgc cgcaccggtt tctacatgag ccttatcggc acgccttccg aacagcaggt
     4441 cgccgatgca tggctcgcct cgatgcagga tgtgggcaat gtcaaagacc aaagcaaaat
     4501 ccccgagttg aacgaatacc aatgcggcac ttatcaaatg cactcgctcg ccgaagcgca
     4561 gcaaatcgcg caaaacgtgt tggcgcgcaa agtggcggtg aacaaaaacg aagagctgac
     4621 gctggatgaa gggctgctga acgcctaatc cgccaaaaat gccgtctgaa caagggtttc
     4681 agacggcatt tgccttttcc gttataatcc ggggttgtgc ggggtcgggt tttaagccgg
     4741 catcgtcctt ccctattttt ttctgtccct tatcggtttt aagcgggttt tttatgtcca
     4801 acagacctac actcctcctc gttgacggct cgtcctacct ctaccgtgcg tatcacgcga
     4861 tggggcaaaa cctgaccgcc cccgacggcg cgccgacggg tgcgctgtat ggcgtattga
     4921 atatgttgcg ccgtttgcgg tcggaatatc cgcacgatta ttgcgcggtg gtttttgatg
     4981 cgaaaggcaa aaatttccgc catcaaatgt ttgaagaata caaggcgacg cgcccgccga
     5041 tgcccgacga tttgcgcccg caggcggaag cactgccgga tttagtgcgc ctgacaggct
     5101 ggccggtatt ggtgattggg caggtggagg cggacgatgt gatcggcacg ctggcgaaac
     5161 agggggcgga acatggtttg cgagtcattg tttcgaccgg cgataaggac atggcgcagt
     5221 tggtggatga gcgcgttacg ctggtgaaca cgatgagcgg agaaacgctg gatattgaag
     5281 gcgtgaaggc aaaattcggc gtgcgccccg accaaatccg cgattatctc gcgctgatgg
     5341 gcgacaaggt ggacaacgtg ccgggcgtgg aaaaatgtgg cccgaaaacg gcagtgaagt
     5401 ggctggaagc ctacggctcg ttgcaaggcg tgatggaaca cgcttcggaa atcaagggca
     5461 aagtgggcga gaatctgcgt gccgccctgc cgcgcctgcc gctgtcgtat gatttggtaa
     5521 cgattaaaac cgatgtggac ttgcacgccg agctttcaga cggcatcgaa agcctgcgcc
     5581 gtaccgcgcc gaaatgggcg caactggttg tcgatttcaa acgctggggc ttccgcacct
     5641 ggctgaaaga agcggaatca aacatgaata ccggctcgac cgatgatgtg ttcggcagcg
     5701 acagcatcgg tgagcaggcg gctttgaatg cggaaatgcc gtctgaaaaa caagctgaaa
     5761 aagccaccgc ccccgaaagg ctggattatc aagccgttac caccgaagcg cagtttgccg
     5821 ctttgttgga caaactgtcg cgggcgaaca caatcggcat cgatacggaa accacgtcat
     5881 tagacgcgat gaacgcctcg ctggtcggca tcagcatcgc tttccaagca ggcgaagcgg
     5941 tttacatccc cgtaggacac agcctgaccg cagcgcctga acagcttgat ttacaagacg
     6001 tattaggccg tctgaaaccg catttgggaa accccgccct aaaaaaaatc gggcaaaacc
     6061 tcaaatacga ccaacacgtt ttcgccaact acggcatcgc cctgaacggc attgccggcg
     6121 acgccatgct cgcttcctac atcatcgaga gccatctcgg acacggcttg gacgaattgt
     6181 ccgaacgctg gctcggcttg gaaaccatta cctacgaatc gctgtgcggc aaaggcgcga
     6241 agcaaatcgg ttttgccgat gtcgccatcg ggcaggcgac cgaatacgcc gcccaagacg
     6301 ccgatttcgc cctgcgcctc gaagcgcacc tgcgcgcgca aatggacgaa aaacagcttg
     6361 aaatgtatga aaaaatggag ctgcccgtcg cgcaggtatt gtttgaaatg gaacgcaacg
     6421 gcgtgcaaat cgaccgcgcc gaactcgccc gccaaagcgc ggaactcggc gccgagctga
     6481 tgaagctcga acaacaagcc tatgccgccg cagaccagcc gttcaacctc aattcgccca
     6541 aacagctgca agaaatcctg ttcgacaaaa tgggcatccc caccaaaggc ctgaaaaaaa
     6601 ccgccaaagg cggcatttcc accaacgaag ccgtgctcga acagctcgcg cccgactacc
     6661 ccctgcctaa aatcatcctg caaaaccgca gcctggcgaa gctcaaatcc acctacaccg
     6721 acaaactacc cgaaatgatt tcccccaagg acggccgcgt gcataccacc tacgcccaag
     6781 ccgtcgccat taccggccgc ctcgccagca acaaccccaa cctgcaaaac atccccatcc
     6841 gcaccgccga aggccgccgc gtgcgccgcg cctttaccgc accgcaaggc agcgtcatcg
     6901 tttccgccga ctattcccaa atcgagctgc gcattatggc gcacctctcc ggcgacaaaa
     6961 ccctgattgc cgcgttccaa aacggcgaag acgtacaccg ccgcaccgcc gccgaagtgt
     7021 tcggcactgc gcccgaaaac gtctcgtccg agcaacgccg ctacgccaaa accatcaact
     7081 tcggcttaat ttacggtatg gggcaatacg gtttggcaaa atcattgggc atcgacaacc
     7141 tttccgccaa aaactttatc gaccgctact tcgcccgcta ccccggcgtc gccgaataca
     7201 tgcagcgcac caaagaacaa gccgccgccc aaggctacgt cgaaaccctg ttcggcagaa
     7261 ggctctattt gcccgacatc cgcaacaaaa acgccaacgc ccgcgcagga gccgagcgcg
     7321 ccgccatcaa tgcccccatg caaggcaccg cgtccgacct catcaaacgc gccatgatag
     7381 acgtgcgaaa ctgtctttca gacggcatcg gcagcaaact ggtcatgcag gtgcatgacg
     7441 aactggtgct ggaagtcgtt gaaaccgaac tggattttgt caaagaaaaa ctgccgcaga
     7501 ttatggcgaa agtggacggc ggattattgg atgtaccgct ggtggctgag gttggcgtag
     7561 gggagaattg ggaagaggca cattgattga aaggtgttat atgctatctt tatttaaata
     7621 aaatttaatt tttggtatat tttttctaaa tgttcctat
//
LOCUS       DAWEWP010000095         7548 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWEWP010000095 DAWEWP010000000
VERSION     DAWEWP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.095"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            24..419
                     /locus_tag="WGN53_001429"
     CDS             24..419
                     /locus_tag="WGN53_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0140413.1"
                     /translation="MFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAGHGI
                     LYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLF
                     AAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WGN53_001430"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WGN53_001430"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ0140414.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFSERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1376..1723
                     /locus_tag="WGN53_001431"
     CDS             1376..1723
                     /locus_tag="WGN53_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ0140415.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="WGN53_001432"
     CDS             1728..2321
                     /locus_tag="WGN53_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ0140416.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDILVGISTSGNSANVIEAIKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="WGN53_001433"
     CDS             2385..2993
                     /locus_tag="WGN53_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ0140417.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3651
                     /locus_tag="WGN53_001434"
     CDS             3001..3651
                     /locus_tag="WGN53_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140418.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3691..4470
                     /gene="map"
                     /locus_tag="WGN53_001435"
     CDS             3691..4470
                     /gene="map"
                     /locus_tag="WGN53_001435"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ0140419.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4625..4924
                     /locus_tag="WGN53_001436"
     CDS             4625..4924
                     /locus_tag="WGN53_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0140420.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5162..5536
                     /locus_tag="WGN53_001437"
     CDS             5162..5536
                     /locus_tag="WGN53_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0140421.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5724..7190)
                     /locus_tag="WGN53_001438"
     CDS             complement(5724..7190)
                     /locus_tag="WGN53_001438"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ0140422.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSTPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1945 a         2290 c         1846 g         1467 t
ORIGIN      
        1 aagaggcgga atgggcgggc agcctgttcg gcctgcacaa atctttcggc ttcctcacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cagttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 attgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcaccgcgcg attggttgcc
      661 gtcggcagac aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt
      721 tttcgtaatt tcgcgcgcca gcattaatcg gcgttcggag aacagttccg ccatatcggc
      781 aagcgtcgca ccgatgcggt gcggcgtttc aaacatcacg acgggaaacg ccgcccgcac
      841 ccatttggca aacagtttcc tgcgctctcc cgatttcggc ggtacaaaac cgttgaaata
      901 aaaatcggat cccgccacac cggccacact caaagccgcc atcaccgcgc ttgcgccgac
      961 aacagggaca actttaaacc cgacctcacg cacgcggcgg gcgagtttcg cgcccgggtc
     1021 gcacacggcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata
     1081 gccgacaatc ttgtccgcca tctgccgttc gttgtgttcg cgcacgctga caagtttgcc
     1141 ctgaatgccg tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgtcg gaggctttct gcaagtgttt
     1321 ctgaaacata aaaaatgccg tctgaaaaac agacattata aaggttaaac cgattatgcg
     1381 cctaaaccac aaacagggcg aggcagggga agatgccgcg cttgccttcc tccaatccca
     1441 aggctgcaca ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgccgca tacagcattt ccccatccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa cagaacaggt tgacaaacgt accgtgccgc ctcgacgcgg tactcatcga
     1681 aggcagccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccgcccat tttgccgaaa gcatccgtgc caagcaggaa gccggaaaag
     1801 tattggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaacgacg
     1861 gcaagattct ggcgtgcggc aacggcggtt cggctgccga cgcgcagcac ttcgccgccg
     1921 aaatgaccgg acgctttgaa aaagagcgca tggaactcgc cgccgtcgcc ctgaccacag
     1981 acacctccgc gctgaccgcc atcggtaacg actacggctt caaccacgtc ttcagcaaac
     2041 aggtacgcgc cctcggacgc gccggcgaca tcctcgtcgg catttccact tccggcaatt
     2101 ccgccaacgt catcgaagcc attaaagcgg cacacgaacg cgatatgcac gtcatcgccc
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttctgctca
     2221 acgtccccca tccgcgcacc gcccgcattc aggaaaacca catcctgctg atacacgcca
     2281 tatgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagcc cccgatgaaa cccaaaccgc
     2401 acaccgtccg caccctgatt gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg aagcgccgcc gtcggcgcga aatccgccgt cgaccgccga accaccggcg
     2521 cgcaaaccga cgacaacgtt atggcgttgc gtatcgaaac caccgcccgc tcctatctgc
     2581 gccaaaacaa ccaaaccaaa ggctacacgc cccaaatctc tgttgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgagaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtat acaactacat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgccgtattg
     3121 ctcatctgta tcaccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcgaca aaacccttca agccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcaacctg ccgacacagc tcaaaccgac
     3421 aggcagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cccacaaacg tcccccgtcc cgaaccccga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacac ccaaagaaaa agaaacgccc aaagaaaacc ataccaaacc ggacaccccg
     3601 aaaaacacgc cgcccaaacc ccataaagaa attctcgaca acctcttctg acccggcacg
     3661 gcaggcacac ccgcaatcca aggaagcatt atgaacggca tcatcatcaa aacccccgaa
     3721 gaaatcgaaa aaatgcgcga gctgggcaaa ctcgtcgccg aagccctcga ctacatcgga
     3781 caattcgtca aacccggcgt aaccaccgac gaaatcgaca aactcgttta cgactaccac
     3841 gtcaacgtcc aaggcggcta tcccgccccc ctgcactacg gcaacccgcc ctaccccaaa
     3901 tcctgctgca cctccgtcaa ccacgtcatc tgccacggca ttcccgacga caagccgctc
     3961 aaagaaggcg acattatcaa catcgacctc accatcaaaa aagacggctt ccacggcgac
     4021 tccagccgta tgtttaccgt cggcaaagtc tcccccatcg cccaacgcct gatcgacgta
     4081 acccacgcct ccatgatggc gggcatagaa gccgtcaaac ccggcgcgac actgggcgac
     4141 gtaggttacg cctgccaaca ggttgccgaa aacgccggct attccgtcgt acaggaattc
     4201 tgcggacacg gcatcgggcg cggtttccac gaagccccgc aagtgttgca ctacggaaaa
     4261 aaaggacagg gctccgttct aaaaccgggt atgattttta ccgtcgaacc gatgatcaac
     4321 caaggcaaac gccacctgcg tatcctcaac gacggctgga cggtggttac caaagaccgc
     4381 tccctctccg cccaatggga acacgaagtc ttggtgaccg aaaccggcta cgaaatcctc
     4441 accgtcagcc ccgcctccgg caaaccctga agccggacgt atccgcccca taaaaacaaa
     4501 caatgccgtc tgaaagaaac ggcagatatg atatataata taaaaacagg cttgacccgg
     4561 cacattacga aaacaaagca aatcggaatt tgccccgcaa ccagacaaac ttaaaggaag
     4621 ttttatgaaa atatttgaaa atatagaaga tgttaaagcc atccgtaaaa agaccgggct
     4681 gaaccagata gacttctggg gcaaggtcgg cgttacccag tctggaggct cgcgctacga
     4741 aaccggccgc aaaatgccca aacccgtacg cgaactgctc cgcctcgtcc atatcgaatg
     4801 catcgatttg gcgaaagtca acaaaaaaga tatggaaatc gccgccctgt tgaaaaaaca
     4861 ccatcccgac ctgtatgccg agttgtccaa acagaccaag tccgaaagaa aaaaacaaag
     4921 ttaaaccgca acctccggat gcccgacagt ttttcatttc cgaaaaacgc aaacaatgcc
     4981 gtctgaaaca ccggacaggt cgccgtatcc cgcctgccgc ccctgcctca aaccgccgaa
     5041 ccgcccgaac ccgccttttt acaaacttta tccaatttcc tgtttatttc aggatacgcc
     5101 gacattagaa tgtcaaacag ctcgaaacgg gcaaactcca catccatcca aaggaataaa
     5161 aatgaaactt ctgaccaccg caatcctgtc ttccgcaatc gcgctcagca gtatggctgc
     5221 tgctgccggc acgaacaacc ccaccgttgc caaaaaaacc gtcagctacg tctgccagca
     5281 aggtaaaaaa gtcaaagtaa cctacggctt caacaaacag ggtctgacca catacgcttc
     5341 cgccgtcatc aacggcaaac gtgtgcaaat gcctgtcaat ttggataaat ccgacaatgt
     5401 ggaaacattc tacggcaaag aaggcggtta tgttttgggt accggcgtga tggatggcaa
     5461 atcttaccgc aaacagccca ttatgattac cgcacctgac aaccaaatcg tcttcaaaga
     5521 ctgttcccca cgttaatcag gcaacaaaaa acagcgtttt cagaaatgaa aacgctgttt
     5581 ttttgaccgt tccattattc gcaaaaggga aaaaacgatt acctgccccg tgtatcaaaa
     5641 cctgccctgc cggatgaagg gcataaccgg cagggacggc atcaacacca tatgggggta
     5701 cggcttttct tgaaagattc ggcttaaata tccaacactt tcgcggtata ggcgataatt
     5761 tcatccgccc tttcggggtt ttcgttcaac ttgatgccgt aacccggtac cagctctttc
     5821 agacggcctt cccaagacgg ggcgcgctcg gggaagcatt ggtgcatcag ccggatcatc
     5881 agcggcacag cagtcgatgc gcccggcgac gcgcccagca atgcggcgag tgagccgtcg
     5941 gcgtgggcga caatctccgt accaaactgg agcacgccgc ctttttcgga gtctttttta
     6001 atgatttgga cgcgttgccc tgcggtgatg agttcccagt cgtcggggtt tgcctcgggg
     6061 tagtattcca gcagggaggc gaagcgttct tctttggttt tacgcaattc gcccagcagg
     6121 tatttggtca gcggcatatt cgcccagccg gcgcacagca taggatagag gttgtccata
     6181 tggatggaca gcggcaaatc cataagcgag ccttgcttga ggaagttgga acggaagcct
     6241 gcataagggc cgaacataag gtggcgtttg ccgtccacgt tgcgtgtgtc gaggtgcggg
     6301 acggacatcg gcggcgcgcc gacggaagcc tgcccgtaca ctttggcgtt gtgttgttcg
     6361 gcggtttcag gggtgctgtt gcggaagaac aggccggaca cggggaagcc gccgtagcct
     6421 ttgccttcgg ggatgccgga tttttgcagc agggtcagcg cgccgccgcc cgcgccgagg
     6481 aagaggaagc gggtacggag ggtgagctgc ccgtcggggt tgcgggtatc ggcggttttg
     6541 agcacccacg cgccgtcgga ttcgcgtttg atgtcttcga cgtggcggtt gaactcggtt
     6601 tttacgccct tgccctgcaa atatttcacc atttggcgcg tcagccgtcc gaaatcgaca
     6661 tccgtgcctt cggcggagta gttggcggcg acgggttggt tttcgtcccg gccgcgcatc
     6721 atcagcggag cccaatcgga aattttgttc cgatcggtgg aaaattccat attttcaaaa
     6781 agtttttggg ttttaaacgc gtcataacgt ttttgaagat aagaacaatg gtcttcattc
     6841 atcaccaaag acatatgcgg cacggcattg atgaaggaat tgtcttccaa cttgccttcc
     6901 gcgaccaacg tcgcccaaaa ctggcggctg acatgaaact gttcggcaat attgagggcg
     6961 cgcgccggat cgataatccc atttgcaccc aacggcgcat agttcaattc gcacagcgcg
     7021 gaatgccccg tgccggcgtt gttccacgcg tttgacgatt ccaacgccac atcttccaag
     7081 cgttcaatca gggtgatttc ccaagacggt tcgagttctt tgagcaaaac gcccaaagtc
     7141 gcgctcataa tgccgccgcc caccaagaca acgtctgtcg cttcagccat ggtttactcc
     7201 taaaaaacag gcatcttctg cccttatggt tatttgccgt actacaaacg cctgaatcgc
     7261 aaaagcaggg aaaaccggca atggtgtgtg tccgagtatg ctgtttcggg gttggaatgc
     7321 gttgcaagta tggcttccga caccgcttca ggggcttgta atatgttatc gtgaatgtag
     7381 tggattttac tgggaaatgc aaagtttttc tgtcgcccgc caagcccggg aactgcgaaa
     7441 tgaaaaataa aaatagttat ttatctatat atatcaaatt tttaatagat aaaaaaccaa
     7501 aattgtttat atattaattt ttaaaagatt gtcagcatat tgcgttaa
//
LOCUS       DAWEWP010000096         7515 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWEWP010000096 DAWEWP010000000
VERSION     DAWEWP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.096"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(104..2893)
                     /gene="ileS"
                     /locus_tag="WGN53_001439"
     CDS             complement(104..2893)
                     /gene="ileS"
                     /locus_tag="WGN53_001439"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ0140423.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVACQKKDFIRLGVLGDWDKPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKGELLSAEDCEHYDKLSDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQERLAGDYYPRYTFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGREGETRHYA"
     gene            complement(3034..3954)
                     /gene="ribF"
                     /locus_tag="WGN53_001440"
     CDS             complement(3034..3954)
                     /gene="ribF"
                     /locus_tag="WGN53_001440"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ0140424.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSDCFQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKCW"
     gene            complement(4481..5776)
                     /gene="tyrS"
                     /locus_tag="WGN53_001441"
     CDS             complement(4481..5776)
                     /gene="tyrS"
                     /locus_tag="WGN53_001441"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ0140425.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVKSIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLYQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(5839..>7515)
                     /locus_tag="WGN53_001442"
     CDS             complement(5839..>7515)
                     /locus_tag="WGN53_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0140426.1"
                     /translation="AFYIRRIKRILPAFFAVLAATLIGGFFLFTKDDFFLLWKSALTA
                     LGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVARKSPRVQFG
                     FLAALCALSLAASFMPSAFDKYYLPHLRACEMLVGSLTAVWMRYRQQRNLAVGRCCAA
                     AGALFSACILSACLFAYSKQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKFFQWKIT
                     VAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSTAAAIVLILLLSLISYHCIEKPFKK
                     WKGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNTGKQCLW
                     GDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYASRVFQNWA
                     ACRAVYRYAEEHLPLYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHKLSSEKQ
                     AVYLMADNLASSYNVQRAYILSSRIPGYRQTLRPDDESTLKANARIRELAAKYPNVYI
                     IDAAAYIPANFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH"
BASE COUNT         1654 a         1922 c         2152 g         1787 t
ORIGIN      
        1 gcaatcttga aacccctccg ccgttataaa gacaaattgc ggcacaaaaa atgccgtctg
       61 aaatgctgtt cggcggtttc agacggcatt tgctcaaact ttatcaggcg taatggcgcg
      121 tttcgccttc tctgccgaca ttctctgcac agcgtttgca gacggtttca tagcctgcaa
      181 ccgcgcccac atcgcgggtg tagtgccagc agcgttcgca tttttcacca tcactggctt
      241 tagcggcaac ggcaagttcg ctgcctactt tcacttctgc tttagacacc agcaaggcaa
      301 agcgcaattc ttcgcccaaa gcgttcaggt agccggccat ttcttccggc gcagtgattt
      361 cggcttcggc ttgcaaggac gaaccgacgg ttttgtcggc gcgcaaaggc tcgatggcgg
      421 cggttaccgc ttcgcgggct tcgcggattg ccgtccattt tttcaccagt tcggcttcgg
      481 ctttttcgtt gatggtcggg aactcgtgcc aggtgtggaa gaggacgctg tcttcttcgc
      541 cgccgccgat gatgtcccac gcttcttcgc cggtaaagca caaaatcggc gcaatcaaga
      601 gaaccaaact gcgcgtgatg tggtacaggg cggtttgcgc gctgcggcgg gcgcggctgt
      661 cggctttggt ggtgtagagg cggtctttca ggatatcgag gtagaacgcg cccaagtctt
      721 ccgagcagaa agaaacaatg tcttttacag caaagtggaa ggtatagcgc ggatagtaat
      781 cgcctgccag acgctcttgc agctgacgtg ccaataccac ggcgtagcgg tcgatttcca
      841 ccatgtctgc ctgttgcacg gcatcttcaa taggattgaa gtcgctgagg ttggcaaaca
      901 ggaagctcaa ggtgttgcgg atacggcggt agctttcggt tacgcgcttg aggatttctt
      961 tggaaatcgc caattcgccg ctgtaatcgg tggatgccgc ccacaggcgc aggatgtccg
     1021 cgccgaactc gttatacact tcttgcggcg cgacgacgtt gccgatggat ttcgacattt
     1081 tgcgcccgtt ttgatccacc acgaaaccat gggtcagcag ctgtttgtac ggcgcgcgac
     1141 ccattgatga ggcgcagccg gtcagcatgg acgactgaaa ccagccccgg tgttgatcgc
     1201 tgccttcgag gtacaagtca gcaggccagt ccaattcttc gcgctgtttc acaacggaat
     1261 aatgggtcga accggagtcg aaccatacgt ccatcgtgtc ggagagtttg tcgtaatgtt
     1321 cgcaatcttc cgcgctcaac aattcgccct tatcgaggga gaaccaagcc tcgatgcctt
     1381 tttcttcgat gcgttgcgcg actttttcca aaagttcggc agagttcgga tgcagctcgc
     1441 ccgtttcttt gtgaacaaag aaagtcatcg gcgtgcccca atagcgttgg cgcgaaacca
     1501 cccagtcggg gcggccttca atcatggctt ccaaacgcgc acgaccccat gatgggaaaa
     1561 attcagtgtc atcaacggct ttgatggctt tatcgcgcag ggttttacca tcggcacctg
     1621 ctttatccat accgacaaac cattggccgg tcgcacggta aatcaacggc gttttgtgac
     1681 gccagcagtg cgcgtagctg tgttcgattt tgctgcttgc caagaggttg ccggtttctt
     1741 ccaaccattg caggataacg ggattggcct cccaaacgct catgccggca acgcgcggcg
     1801 tttcactgat gtatttgcct tcggcgttga cggggttgta aagctcgatg ccgtatttat
     1861 tgcagacggc gtagtcttcc aaaccatgag caggcgcagt atgcaccaaa ccggtaccgg
     1921 cgtcggtggt aacgtggtcg ccgttgagca tgggaatatc gcgttcgagg aacggatgat
     1981 tcatgtgcag gttttccagc ttgtcgccgg tggtttcggc aagaatagca atgccgtctg
     2041 aaaaaccgta acgtttgagc gcgtcttctg ccaaatcttt cgccagcacc aatttgcctt
     2101 tgggcgtatc gattaattga tacaccacgt ccgcaccggc agacacggct tggctcgctg
     2161 gcagcgtcca aggcgtggtc gtccagatga cggcaaacgc tttgccctcg atgcctgcca
     2221 aaccgaatgc agcggcaagc gcagcggtgt ctttaaacgg atagcccacg tcaatcgcag
     2281 gcgatacttt gtctttgtat tccacttcgg cttcggccaa tgaagagccg cagtccaagc
     2341 agaactgaac cggtttcgcg ccgcggtaga gatagccgga tttgtagatt tcgccgagca
     2401 tacgcacggt atcggcttcg gttttaaaat ccatggtcag gtagggcttg tcccagtcgc
     2461 ccaacacgcc cagacggatg aagtcttttt tctggcaggc aacttgttcg gcggcatatt
     2521 cgcggcacaa ttcgcggaaa cgcgctttgg gcatgtcttt gccgtgcagc ttttctacca
     2581 tcacttcgat aggcaaaccg tgacagtccc aacccggcac ataaggcgcg tcaaaaccgg
     2641 cttgggtttt gctgcggata atgatgtctt tgagaatttt gttgacggcg tgaccgatgt
     2701 ggatgtcgcc gttggcatac ggcgggccgt cgtgcaggat gaacttcgga cgacctttgg
     2761 cgatttcgcg cagtttttgg tagcgttttt gctcgtacca gcttttcagc catgcaggct
     2821 cgcgcttggc aagattgccg cgcatcggaa acgggctttc gagcaggttt acggttttgc
     2881 tgtaatcggt cattttttaa tctctattgt tacaatattt cggttcagac ggcattgcgc
     2941 ctcaaacagt atttcacaac gggaaacccc tatgccgtct gaacagttaa aaggttgatt
     3001 gtagcccaat cggatggttt gtataaggtt tttctaccaa cactttgcgg cttccatatc
     3061 ggcttcaatc tgccttttca gttcttccat accgtcaaac ttttcctcat cgcgcagttt
     3121 gtgcaggaag cggacgttca accgttgtcc gtacaaatcg ccttgaaagt cgaacaggtg
     3181 gacttccagc ttttgaaaac agtcgctatc aacggtggga ttgaagccga agctcgccac
     3241 gccgcgccgc gtgccgaacg cgccgtccgc ttcgacgacg aacacgccgc cgagtgcata
     3301 acggtggcgg ggcaggcgga tgttggcagt cggggcgttt aaggtgcgtc cgagttttct
     3361 gccgtgcacc accctgccgc tcaagacgta gtcgtgtccc aaaagttttt tcgcataggc
     3421 aaggttgccg tctgaaaggg cttgtcgcac ggcggtactg ctggtgcgga tgtcttcgac
     3481 gatgacggaa ggcgtacgct cggtctgcat atcgggctgt tgtgccaaaa gttcaaaaca
     3541 gccttcccgc cccgcaccga aacggaaatc gtcgccgacg agcaaataac gcgtattcaa
     3601 ggtttgacgc agcaggcggt cgataaaccc ttgcgcggat atttcggaaa aattttgatc
     3661 gaaacgcaaa acccagacgg catcgacaca gcctgtgcct tccaataatt cgagcttggt
     3721 gcgcaggggg ctgatccgac acggtggcat cctgccggtg cggagtgcga aaaattcttt
     3781 gggttggggt tcgaaaacga cggtcacgac gggtaatccg cgcgtatcgg cttcgaggcg
     3841 gagtttttgg aggatgtgtt tgtgtccgag gtgtacgccg tcaaaattgc ctatggttac
     3901 ggcggccccg tgcggaaagt cgggcgcgtt gtgccgcccc agcctgattc tcattgttat
     3961 gttcgggtat gtggtgaaac aggcggtcat tgtaaacggt attgcggttt atagacagtg
     4021 tgccgcccgt tacgcccgcc cgacgcggga aaagtaggca aatttcccgc agccgaacgc
     4081 gccaaacgca taaagcgcgc agcaggcgcg gtgctatgtg ttgaaacatc gccccaaacc
     4141 tgatgcaaca tcagtgaaaa tcgttctttt ttaaccaggt aaaccgagac aagcaaccct
     4201 ccgccgtcat tcccgcgcag gcgggaatcc ggacctgtcc gcacagaaac ttatcggata
     4261 aaaacagttg ctcaaacccc gagattctag attcccactt tcgtgggaat gacggttcag
     4321 ttgcgttccg acaacaccgt aatcttgaaa ctcgtcattc ccgctcaggc gggaatctag
     4381 aacgtggaat ctaagaaacc gttttgcccg ataagtttcc gtgcggacag gtccggattc
     4441 ccgcctgcgc gggaatgacg gcatttctgc ggcaatcgga ttatttccaa accaaaagcg
     4501 cgtggttgcg tttgccgcgc cgaaggatgg tgtatttgcc gaaacgtttg tgttcgccgt
     4561 tcagcaggca ggcatcgtcg gggcgttcgg cggcgtggtt ggggttgttg gcttcggcag
     4621 gtttgccgtt gagcaaaacc gctttgctgt tcacaaagcc gcgcgcttct ttattggagg
     4681 atgccaaacc ggttttgacc aaggcttcga cgacattgat gccgtctgaa acttcaaatg
     4741 caggcaggcc gtcgagggcg agctgctcga agtcgctttc ggtcaggctg ctttggtctt
     4801 cggcaaacag gctttcggaa atgcgctgcg cggcggcaag ggcttcttcg ccgtgaatca
     4861 ggcgggtcat ttcttcggcg aggatgcgtt gcgcttcggg cttgctgccg ctggctttat
     4921 ctttcgcttc gatagcgtcg atttcttcga tggacaggaa ggtaaagtat ttcaggaatt
     4981 tatacacatc ggcatcggcg actttcagcc agaattggta gaactgatag ggcgaggttt
     5041 ttttcgcgtt cagccacacc gcgccgcctt cggttttgcc gaatttggtg ccgtctgatt
     5101 tggtaaccaa aggcagagtc agtccgaaca cttgtttttg gtacaggcgg cgggtcaggt
     5161 cgataccggc agtgatgtta ccccattggt cggagccgcc gatttccaaa accgcgccgt
     5221 ggcgtttgtt caactcggcg aagtcgtaac cttgcagcag ggaataggcg aactcggtga
     5281 aggaaatgcc tgcgccgtcg cggtcgatgc gctgtttgac ggattctttg ttcagcatgg
     5341 cgttgacgga gaaatgcttg ccgatgtcgc gcaggaagtc gaggcagttc attttgccga
     5401 accagtcggc gttgttcgcc ataatggcgg cgtttccgcc ttcaaagctc aagaaagggg
     5461 ttaattggct gcggatgctt ttcacccagc cggcaacggt ttcggcggaa ttcaagctgc
     5521 gttcggcggc tttgaagctg gggtcgccga tcataccggt cgcaccgccc accagtgcaa
     5581 tcggcgtatg ccccgcctgt tggaagcggc gcaatgccaa tacgggcagc aggtgtccga
     5641 tgtgcaggct gtcggcggtc gggtcgaagc cgcaataaag ggaaattttt tgttcgttca
     5701 acaaagcgtc taaggcttcg atgtcggtgg tttgcgcgat taggccgcgc gattgcaggt
     5761 cttggatgac gctcatcggt ctctttcaaa aaaaattagc ggttttgcaa accgccgatt
     5821 gtaacaaatt taagcgaatc aatggttatg gcgcgtatcg agaaaccgtt gtttttcgga
     5881 aaaacgcttc gccaattccg tgccgccgta agggttgatg tggtctttgt ccgagtaaac
     5941 cggcaatccg ccgatttgga aatttgcggg gatataggcg gcggcatcaa taatatagac
     6001 gttggggtat ttggctgcca attccctgat gcgtgcattg gctttcagag tgctttcgtc
     6061 gtccgggcgc agggtttggc ggtaacccgg tatgcgtgaa gacaagatat aggcgcgttg
     6121 tacgttgtaa gacgaggcaa ggttgtccgc catcaggtaa acggcttgtt tttcggatga
     6181 gagtttgtgc agcatacggt cgaatttttg gaaaaaaccg gcatcgtagg caagggagcg
     6241 gctgttttcg ggcatttggc tgccccagcg catcgccaaa accacttttg aatacagggg
     6301 caggtgttct tcggcatagc ggtaaacggc gcggcaggcg gcccagtttt ggaacacacg
     6361 ggacgcgtag ccttccacat aggcgcaagc gtcggcggaa accatagtgg cggaccattt
     6421 ttcttttttg cccacggcat cgaagaatgt tttgtaatgg tcggcgtggg agtcgcccaa
     6481 aaccagcagc tccggctgtt tttccgtatc cccccacagg cattgtttgc cggtattgtt
     6541 gtggcaggag gtgttggaac gcgtcagccc caagcggtcg tattgcgcca taaacggcag
     6601 cctcatcgcg aaaaacgagc ccgcccccaa aatgagcata ggcaaggcat aaatccataa
     6661 aacggattgt gcgaacgaac ctttccattt tttaaacggc ttttcgatgc agtgataaga
     6721 aatcagggaa agcagcaata tcaggacgat tgctgccgct gttgaataag gcggcaggtt
     6781 gtccgggccg atatagcgca taaaggccaa tatcggccaa tgccacagat aaagcgaata
     6841 ggaaatcaaa ccggcggcaa cagtgatttt ccattggaaa aattttttaa gcgggtgttc
     6901 gtaatgattg aaataaatca gcgcggcaac agccagacag ggaatcaaag cggcgggtcc
     6961 cgggaaatag gcggtttgtt tcgaataggc aaacaggcag gcggacagta tgcacgcgga
     7021 aaacaatgcg ccggcggcgg cacagcatct gccaacggcg agattccgct gttgccggta
     7081 ccgcatccac acggcggtca gcgatccgac cagcatttcg caggcgcgca ggtggggcag
     7141 gtaatattta tcgaacgcgg aaggcataaa ggaagcggca aggcttaagg cgcacaatgc
     7201 ggcgaggaag ccgaactgta cgcgcgggct tttgcgggcg acaagcagca gcaatatcgg
     7261 aaagacaaag taaaattgtt cttcgaccga caaagaccaa atgtgcagca agggcttttc
     7321 ttcctgcgcg ggatcgaaat aatccttccc ccttgcaaaa tacaggttgg aggcgaaacc
     7381 caaggcggtc agcgcggatt tccacaaaag aaagaaatca tctttggtga ataaaaagaa
     7441 gccgcctatc agcgttgccg ccaatacggc gaaaaatgcg ggcaaaatcc gtttgatgcg
     7501 gcggatataa aatgc
//
LOCUS       DAWEWP010000097         7492 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWEWP010000097 DAWEWP010000000
VERSION     DAWEWP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.097"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(16..771)
                     /locus_tag="WGN53_001443"
     CDS             complement(16..771)
                     /locus_tag="WGN53_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ0140427.1"
                     /translation="MKYVRLFFLGAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(896..2068)
                     /gene="nirK"
                     /locus_tag="WGN53_001444"
     CDS             complement(896..2068)
                     /gene="nirK"
                     /locus_tag="WGN53_001444"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ0140428.1"
                     /translation="MKRQALAAMIASMFALAACGGEQAAQAPAETPAASAEAASSATQ
                     AAAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAASFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAIAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASASEKSVY"
     gene            2442..4697
                     /locus_tag="WGN53_001445"
     CDS             2442..4697
                     /locus_tag="WGN53_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ0140429.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELVAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPNETFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            4876..5409
                     /locus_tag="WGN53_001446"
     CDS             4876..5409
                     /locus_tag="WGN53_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ0140430.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5472..6656)
                     /locus_tag="WGN53_001447"
     CDS             complement(5472..6656)
                     /locus_tag="WGN53_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ0140431.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDAQRVADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6696..7100)
                     /locus_tag="WGN53_001448"
     CDS             complement(6696..7100)
                     /locus_tag="WGN53_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ0140432.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1664 a         1800 c         2090 g         1938 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agacatattt
       61 ggattgcagg ctggtgcgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccgata gacgcgccgc tgcaaaacat ttgcgcgttg gcattgccgg aagaaagcag
      181 gctgctgttg aaatcttccg tccattccca aatcagcccg tgcatatcat aaacgcccca
      241 gtagttcggg cggcctttgc cgacatcgtg caggcctttc cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      421 ggcgttggcg gcaaaccagg aaacatttgt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      541 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagccgccgc
      721 ttgagtgccg gcgagtgccg cgccgaggaa aaataaccgg acatacttca taaagcctcc
      781 tgacaggcgg ttaaaatcaa tcttccgaaa ggaaagattg gttgttaaaa aaccactgcc
      841 gtgcgtaatg aagtacagcg gcagtggttc gtcccgctaa tgacgggtat ccaatttaat
      901 aaacgctttt ttcggatgca gaggctgccg gggcagaagc tgcgggagcg gaagcagcag
      961 gagctgcacc gttaccggcg taagcggtat cactcaattt ttgagtcatg atttcagggt
     1021 tttccgcacc ctctactttc aattggccca acgcgccttt gttgaatgcg cggaagatgg
     1081 agtggtcgac caaagtgtag ctgcccggga tgtcgacttt gaattcgacg atggcggaac
     1141 cgccggcagg aacgatggtg ctttgtacgt tttcgttaat cagtttgccg ccttcaacat
     1201 aaactttgtc gaagatttcg ccgatgacgt ggaaggaaga caccaggttc gggccgccgt
     1261 taccaacgta catacgcacg gtttcgcctg ctttggcttt cagggcgttg tcgccggcga
     1321 tagcacctac gtgaccgtta aatacaacgt attcaggttg ttcggcgatg gctttgtcca
     1381 tatcgaacgg ttgcaggcct tgcgcgcctt ttttgccttt ggtgtagaag tcgccttgga
     1441 cgatgtagaa ctctttatcc actttcggca gaccttcttt aggctcgact aaaatcagac
     1501 cgtacatacc gttggcgatg tgcataccga ccggcgcgac ggcgcagtgg tagatgtaca
     1561 ggcccggttg cagggctttg aagctgaatg tagaggtacg gcccggggca gtgaaggagg
     1621 ctgccgcgcc gccgccttgg ccggtagctg cgtggaagtc gacgttatgg ggaacggtag
     1681 aagaaggatt gttggagaat tcgacttcta cggtatcgcc ttcgcgcacg cggatcatac
     1741 ggcccggaac gtcgccgtca aatgtccagt agcggtattc cacaccgtcg tccattttca
     1801 tggttttttc gacggtttcc attttcacgc ggactttggc agggtagtcg cggtcgattg
     1861 caggaggtac ttcgggagcg tgggtggtaa ccgcatcgat aacgggcagt tcgcctgccg
     1921 gagtttcggc ggcagcttgt gtagcggagc ttgcagcttc agcggaagcg gcaggggttt
     1981 cagcaggggc ttgagcagct tgttcgccac cgcaggcggc taaagcaaac atggaagcga
     2041 tcattgcggc taatgcttgg cgtttcataa tgttttcctt ttgtaagaaa agtagggaga
     2101 ttggttaaga actggcgtga ttatgcgttt gccccattac aggtattttg atttaagtca
     2161 aataattcat aaaatatgaa cttaaaatga tacttctaat actttataac tactaaaaat
     2221 tacaataaat aagctgattt gcttaagggc ttcggcaaat caggcaaagg cagggtagtt
     2281 gccgactaaa gaataatgat agtcattatc atatatgtat ttgttttata tgcgagtttg
     2341 tttgaacaaa tgtttatttg tgcggtaaac cgtgctacaa tctttaacat tcatattctg
     2401 tgaattttaa acaaccactt ttaccaaaag gagctctaaa aatgggacag tacaagaagc
     2461 tatggtactt gctgtttgcc gttctggcgg tatgctttac cattcttggc tatatgggca
     2521 gcgaggttta taaaaaagcc ccgccttacc ctgaacaggt cgtttctgca tcaggcaagg
     2581 ttctgatgac gaaagacgat attttggcag gtcagtctgc atggcaaagt accggcggta
     2641 tggaggtcgg ttcgattctg ggtcatggtg catatcaggc tccggactgg actgcggact
     2701 ggctgcatcg tgagttggtt gcatggttgg atttgactgc gcaacagact tacggcaaaa
     2761 aattcgatga agtttctcct gaggaacaag ctgtcttgaa aacacgcttg gctgacgaat
     2821 accgtaatca aagccgtgtg aaagaagatg gttctgttgt catcagcgat acgcgtgtaa
     2881 aagctatcga aagcatcctg ccttactacc acggtgttta tggcgacgat cctaagctgc
     2941 aaaccactcg tgaacacttt gcaatgaaaa acaatacatt gccaagtcaa gaggcacgtg
     3001 aaaaactgtt cgacttcttc ttctggactt catggtctgc ttcgaccaac cgtcctaatg
     3061 aaacctttac ctataccaac aactggccgc acgagccttt gatcaacaat gtgccgacta
     3121 ctgaaaacta tatgtggtca ttcaccagcg ttgttcttct gttgatgggc atcggtttgt
     3181 tgatgtgggg ttattctttc ctgaccaaac acgaagaagt ggaagtgccg tctgaagacc
     3241 cgatttccaa aatccaactg accccttcac aaaaagcatt gggcaaatac gtcttcctga
     3301 cggttgccct gtttgtggta caagtattgc tgggcggtct gaccgcgcac tacaccgtcg
     3361 aaggtcaggg tttctacggc attgacgagg cgttgggctt tgaaatgtcc gactggttcc
     3421 cttacgccct gacccgtact tggcacatcc aatccgccat cttctggatt gcaaccggct
     3481 ttttgacggc aggtttgttc cttgcgccga ttgtcaacgg cggcaaagat cccaagttcc
     3541 aacgtgcagg cgtgaacttc ctctatatcg ccctcttcat cgtggtcggc ggctcttacg
     3601 cgggcaactt cttcgcgttg acgcacatcc ttcccccgga atttaacttc tggttcggac
     3661 accaaggtta cgaatacctc gatttgggac gtttctggca actcctgctg atggtcggcc
     3721 tgctgttgtg gctgttcctg atgttgcgtt gcacggtttc cgccttcaaa gaaaaaggcg
     3781 tggacaaaaa cctgctggca atctttgtcg cttctatggt cggtgtgggc gtattctacg
     3841 caccgggact gttctatggc gagaaatccc cgattgccgt aatggaatac tggcgttggt
     3901 gggtggttca cctgtgggtg gaaggcttct ttgaagtatt tgccactgcc gcttttgcct
     3961 ttgtcttcta caatatgggc tttgtccgcc gcagtaccgc cacagcttct actctggccg
     4021 ctgccgccat ctttatgttg ggcggcgttc cgggtacgct gcaccacctg tacttctccg
     4081 gctctacctc cgcctctatg gcaatcggtg cctgcttctc cgctttggaa gtcgtgccgc
     4141 tggtgttgct gggccgtgaa gcatacgagc attggtctta ccaacacctg tccgactggg
     4201 cgaaacgcct gcgttggccg ctgatgtgct tcgtggcagt cgccttttgg aatatgatcg
     4261 gtgccggtgt attcggtttc ctgattaacc cgccgatttc cctgttctat atccaaggtt
     4321 tgaatacttc tgctgttcat gcgcacgccg ccttgttcgg tgtgtacggt ttcttggcat
     4381 tgggcttcgt attgctggtt gcccgttatt tgaaaccgaa cgcgcagttt gacgacaaac
     4441 tgatgacttg gggcttctgg ctgctcaacg gcggcttggt cggtatgatt gccatcagcc
     4501 tgttgccggt tggcgtgatt caggcatacg cctccatcac gcacggcctg tggtatgcac
     4561 gcagcgaaga gttcctgcaa atggaaatcc tcgacacctt gcgttgggta cgtacggcag
     4621 ctgacttggt ctttatcggc ggtgcaatct gcgttgccat ccaagccact aaaatcgtat
     4681 tcggccgcga caaataagtt ttgcggtatg aaatgagcaa ctgcacccgt tttcgggtgc
     4741 agttttttat tgcggattgg gacgggaatg gaaaaggatg tggatttttt attttaatcg
     4801 gctatacaat tgctgctttc atgccgtctg aagcacgttt cagatggcat cggtttacat
     4861 cagataagga taaaaatggg tatttacgat tttcaaatga aagatgcgga aggcaatgcg
     4921 gttgatttgt caggctatcg cggcaaggtt ctgctgattg tcaacacggc aacgcgttgc
     4981 ggtctgaccc cgcaatacga ggctttgcag aagctgtatg cacaatatac cgcagaaggc
     5041 ttggagattt tggattttcc gtgcaaccag ttccgcgaac aggctcccga aagcagcgga
     5101 gaaattgccc aagtgtgtat gatgaagttt ggcacgaagt ttaagatttt cgacaaaatc
     5161 gaagtcaatg gagcaaatac cgcgcccttg tatgcctacc tgaaatccgt caaaccgcaa
     5221 gataaaggca atcatctgtt taaagatttc gtgttgaagc tcgcagcttt gggagagaag
     5281 cgcgacgaag gcgacatcaa atggaacttt accaaattcc tcgtcaaccg cgacggcgaa
     5341 gtggtcgagc gttttgcgcc tagtgttacg cccgaagaaa tcgaagccga tattcgggca
     5401 ttgctgtaat ctgccgccgt cttgggaaat gccgtctgaa ggcttcagac ggcatttgtc
     5461 cgtttgcggg tttattccga aatatcggca acaccgcttt cggggaagat gggcaaatcc
     5521 ggcggtggga gcagggtatc caaagtcagg gcggacgcat cgagtgtggg gtagccttca
     5581 aacgcgaccg aataaccgct gtttccgttc atacggattc gggcgtgcgc gcccagaggg
     5641 aaagtgattt tgtcggcaat gtgcccgaag ggaaagcccg tcagcacggg gattttcgcc
     5701 gtgcgcgaaa catggttggc aacggcggaa aaatcataag acggatcata gacatctcga
     5761 attttttcca tacggaaatt gccgaacacg atggcgcgct gtttcttcaa aatacccgaa
     5821 agatacagcg tattgagcat acgttcgatg cggtagggct gttcgccgac atcttcgagg
     5881 aacaaaatgc cgccgtcgat gtcgggcata taaggcgtgc cggcgagcga ggcgaggacg
     5941 cttaagttgc cgccccacaa tatgccttcg gtttcgacat cggcgcgttg gatataagga
     6001 acatcaacgg tcaggcggtt ttgggttgca cccttgataa aggcatccat cgtaaacgca
     6061 ccgggggcgg gtttgccaaa atcgctataa gccatcgggc cggcaaaact catcatattg
     6121 ccttttgcca acaatgccag ctggacggcg catacgtcgc tgaatccgaa aaacagcgtg
     6181 ccgtgttcgc gcatccttgc gccgagcgaa gcaaaatcga tatgcggcag aatccgcgcc
     6241 gcaccgtaac cgccgcgcaa gcccatcagc actttaggcg tggcgacgcg gccggaagcg
     6301 acctcttgga aatcggcgac acgttgtgcg tccgtaccag caaaccgttg gaagcggcgg
     6361 ctgcccgcct gttggttggt tacggtaaaa ccggcattgt aaaggcgggt taaggctgtg
     6421 ttgacgcggt tggtgtcttc ggcaaagcct gacgaagcga caacgcgcag aaggttgccg
     6481 tccgaacttt gacggcgtgg cgtttggaga ggcacggttc gggctttcac aacagaatgg
     6541 gaagagggaa ggggcggaac ggatgtggcg gatgtgccgc aagcctgaag cagtcccgcg
     6601 ccggcggcgg cggtgcaggt tttcagaaaa cgacggcggg aggtcggttc ggtcataatt
     6661 tccctttcat acggtttcag acggcatcgg cgggcttaaa gcagcttttt aagctgttcc
     6721 gtccaatctt gcggcagggt cataccgtgt tttcggacgg ggtttgccca aatcggcgca
     6781 gggaaggacg catcgttttc aaagcgggcg ataatatgcc aatgcaggtg cggcacgaca
     6841 ttgcccaagc tggcgaggtt aatcttggca ggttgaaaca cttggcgcat agcggcttcg
     6901 actttgtaca ccatttccat caattcgccg cgttccgctg ccgaaaggtc ggtcatttcg
     6961 gcaatatgct tacgccaaat gacgcggcag aatgcaggcg aaccgctgtc gttatggacg
     7021 gcgatgacgc gcagcttggg ggtttgcagc aaaatatctt catcttgtgc cgtgcagatg
     7081 gggcagggtt gggcggtcat ttttccttat tcctgaaagt gtttaaaaaa cggtagggta
     7141 atttttaata tttcaatctg tctgtccgca ttgccgttaa ttgtaccact tcatagggcg
     7201 gtgctctatg tgtcgtcctg tgtgttgaaa catatcggac gcgtttgaac agttgtgcgg
     7261 caaactgttt tagcggcagg caagagatgc cgtctgaagc ccattgtccg tcattcccac
     7321 acaggcagtt gctacggttg ctgaagcccc ttagccgtcg cgcaggcggt tgctacggtt
     7381 actaaaatcc cgtcattccc gcgcaggcgg gaatccagtc cgttcggttt cagttgtttc
     7441 tgataaatct tgcagcgttg ggtttttaga ttcccgcttt cgcgggaatg ac
//
LOCUS       DAWEWP010000098         7362 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWEWP010000098 DAWEWP010000000
VERSION     DAWEWP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.098"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            46..990
                     /gene="tehA"
                     /locus_tag="WGN53_001449"
     CDS             46..990
                     /gene="tehA"
                     /locus_tag="WGN53_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEZ0140433.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAAASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYVGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GVLSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRG"
     gene            complement(1064..1747)
                     /locus_tag="WGN53_001450"
     CDS             complement(1064..1747)
                     /locus_tag="WGN53_001450"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ0140434.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDKDIMGLEIPTGQPLVYKLDDN
                     LKVLEKFYL"
     gene            2044..4347
                     /gene="parC"
                     /locus_tag="WGN53_001451"
     CDS             2044..4347
                     /gene="parC"
                     /locus_tag="WGN53_001451"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ0140435.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSSK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRVVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAVFRLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEIQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTTFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTVDSGETVLPPVAVYASSLINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNTGESMTHTAVSSEAEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4368..5885
                     /locus_tag="WGN53_001452"
     CDS             4368..5885
                     /locus_tag="WGN53_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0140436.1"
                     /translation="MVISNPRELEKLKDRIPNLINIARIAIVLPLLIMHIFGLEVSGH
                     ENLYASWTTWAFYLWLAISCWMIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVGSSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
                     TASATFILVAASYLSAIFTSLSVKYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAV
                     VVINVEHQTILFNKKAKDLLPMLEIGQHTVLFDPITVLWDKASSRTFERNIDTPALTA
                     RIRAVPMNKEQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFW
                     AEFKQEFLLGHPDAAGCIRPDIQGGSLTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWHTPTSAI"
     gene            5937..6404
                     /locus_tag="WGN53_001453"
     CDS             5937..6404
                     /locus_tag="WGN53_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEZ0140437.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNVDQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            6508..7260
                     /locus_tag="WGN53_001454"
     CDS             6508..7260
                     /locus_tag="WGN53_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0140438.1"
                     /translation="MTDILIDNTATETVRTLIQAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1672 a         2214 c         1912 g         1564 t
ORIGIN      
        1 cactatatct gtgcgttacg acggcatgcc gtctgaaggg tgtttatgtc tgcatctaag
       61 aaatttccga ttcctttgag ctatttcagc atcgcgctgg gcttgtttgc cttggggctg
      121 tcgtggcgtt acggcgcgtc tgtcgggctg ctccccgcct tggccgccga atcgctgctt
      181 gcggcggctt cggttgtctg gctcttgctg gtggcggcat acctgatcaa aatgtttgcg
      241 taccgaaacg attttttgtc tgatttgcgc gacttggtgc aatgctgctt catcagcgcg
      301 attccgatta ccgctatgct ggagggactc gcgctgaagc cctatcaggc aggcgcggcg
      361 gcagtcctga tttatgtcgg cgttgccgga cagttggcgt tttcgatgta tcgggcggcc
      421 ggtctgtggc gcggcctgca ttccttggag gcgacgacgc cgattattta tctgcctacg
      481 gttgcgacaa actttgtcag cgcgtcatct ctggcggcgt tggggcatca tgattatgca
      541 gctttgtttt tcggcgcggg tatgttttcc tggctgagct tggaagcctc catcttgggc
      601 aggctgcgca cggcggcacc ggtcggcacg gcggcgcgtg gcgtggtcgg catccagctt
      661 gcgcccgcct ttgtcggctg cggcgcgtat tttgccgtcg gcggtaaagt cgacggtttt
      721 gcgttggcat taatcggcta cggctgcctg cagcttttgt tcttgctacg cctgacccgc
      781 tggttttggg aaggtggttt tacgatgagc ttttggggat tttcattcgg tttcgcggca
      841 atggcaggat gcggtctgca tctggcggct tctggcgtat tgtcgggctt ggggctgacg
      901 cttgccaccg ccggatcggc aggcgtggcg ctgctgcttg tcggtacgct gcaccggata
      961 gcgacggggc gtttcttggt acgcggctga tgcgttttgc cgccttgtca aaaatgccgt
     1021 ctgaacgctc aagccttcag acggcatttt ttatttcaca cccttacagg tagaattttt
     1081 cgaggacttt caaattgtcg tccaatttgt acaccaacgg ctgaccggtc gggatttcca
     1141 agcccatgat gtctttgtcg gaaatgccct cgatgtgttt tgccagcgcg cgcagggagt
     1201 tgccgtgcgc cgccaccaag acgcgtttgc cgctcaaaat cgcgggggcg atttggtctt
     1261 cccaaaacgg caatacgcgc tccagcgtta ctttcaggtt ttcgccgtcg ggtacgacat
     1321 cggcaggcag atgggcatag cggcggtctt tgtgtgcgga aaactcatcg tctttgtcca
     1381 aaagcggcgg cagggtgtcg tagctgcgcc gccagatgcg gacttgctcg tcgccgtatt
     1441 gttcggcggt ttgttttttg tccaggcctt gcagttggcc gtagtggcgt tcgttcagcc
     1501 gccatgtttt gatttgcggt acgaacagtt ggtcggattc ttccaaaacg atgttgcagg
     1561 tcttaatcgc gcgggtcagg acggatgtga aggcgatgtc gaactcatag ccgttttctt
     1621 tcagtttttt accggcggcg gcagcctcgg caagcccctg ctcgctcagc ttcacgtcgc
     1681 gccagcctgt aaacaggttt ttcgcgttcc attcgctttg tccgtggcgg ataaatacca
     1741 gttccatatc gtctccaatg tgtgaaagtg ggaaagcctt atttataaca tattttcaca
     1801 tttcccgtat ttgattcaga ttcagacacg cgcccactat ggtttgccgt tttgatttac
     1861 aataatgtcc tttgttttac attccgcata cacaatgttt caacacacag gacgacacat
     1921 aaagcaccgc cctatgtgtt gccctgattt ggaaggggtt gccccctccc aaataaagtc
     1981 tgattctacc gccccgaagg acagatgtcc aagtggcggg gtttcaaccg aaaaggaaat
     2041 acgataaata tgcaaccgca cgctccacat accgattcca atacgctgat gctcggccga
     2101 tacgccgaac gcgcctatct cgaatacgcc atgagcgtgg tcaaaggccg cgcgctgcct
     2161 gaagtttcag acggccaaaa gcccgttcag cggcgcattt tgtttgcgat gcgcgatatg
     2221 ggtttgacgg cgggggcgaa gccggtgaaa tcggcacgcg tggtcggtga gattttgggt
     2281 aaataccatc cgcacggcga cagttccgcc tatgaggcga tggtgcggat ggcgcaggat
     2341 tttaccttgc gctatccctt aatcgacggc atcggcaact tcggttcgcg cgacggcgac
     2401 ggtgcggcgg cgatgcgtta caccgaagcg cggctcacgc cgattgcgga attgctgttg
     2461 tccgaaatca atcagggaac ggtggatttc gtgccgaact acgacggcgc gtttgacgag
     2521 ccgctgcacc tgcccgcccg cctgcctatg gtcttgctca acggcgcgtc aggcatcgcg
     2581 gtgggcatgg cgaccgagat tccgtcgcac aatttgaacg aagtgacgca ggcggcgatt
     2641 gcgctgttga aaaagccgac gctggaaacc gccgacctga tgcaatatat tcctgcgccc
     2701 gattttgccg gcggcggtca aatcatcacg ccggcggacg aattgcgccg gatttatgaa
     2761 accggcaagg gcagcgtgcg cgtgcgtgcg cgttatgaaa tcgaaaaatt ggcgcgcgga
     2821 caatggcgcg tcatcgtaac cgagctaccg ccgaacgcca attcctccaa aatccttgcc
     2881 gaaatcgaag agcagaccaa cccgaaaccg aaagcaggca agaagcagct caaccaagac
     2941 cagctcaata ccaaaaagct gatgctggat ttaatcgacc gcgtgcgcga cgagtccgac
     3001 ggcgaacatc ccgtacgact ggtgttcgaa ccgaaatcca gccgcatcga taccgatacc
     3061 ttcatcaaca cgctgatggc gcaaacttcg ctggaaggca atgtgtccat gaacttggtg
     3121 atgatgggtt tggacaaccg ccccgcgcag aaaaacctga aaacgatttt gcaggaatgg
     3181 ctggatttcc gcgtcgttac cgtaacacgc cgtctgaaat tccgtttgaa ccaagtggaa
     3241 aaacggttgc acatcctcga aggccgtctg aaagtctttc tgcacatcga cgaagtgatt
     3301 aaagtcatcc gcgaatcaga cgacccgaaa accgatttga tggcggtgtt caggctgacc
     3361 gaaatccaag ccgaagacat tttggaaatc cgcctgcgcc agttggcgcg tttggaaggt
     3421 ttcaaactcg aaaaagaatt gaacgaattg cgcgaggaac aaggccgtct gaacatcctt
     3481 ttgggcgacg aaaacgaaaa acgcaagctg attatcaaag aaatacaggc ggatatgaaa
     3541 caatacggcg acgcgcgacg cacgctggtg gaagaagccg gacgcgccgt gttgacgcag
     3601 accactgccg acgaacccat cacgctgatt ctgtcggaaa aaggctggat acgcagccgc
     3661 gccggacaca accttgattt gagccaaacc acgttcaaag aaggtgaccg cctcaaacaa
     3721 acccttgaag gccgcaccgt tttacctgtc gtcatcttgg attcatcggg cagaacctac
     3781 acgctcgatg ccgccgaaat ccccggcggg cgcggcgacg gcgtaccggt ttcatcctta
     3841 atcgagctgc aaaacggcgc gaaacccgtt gcgatgttga caggattgcc ggaacaacat
     3901 tatttattat caagcagcgg cggctacggc ttcatcgcca aactgggcga tatggtcggg
     3961 cgcgtgaaag cgggcaaagt agtgatgact gtggatagcg gcgaaaccgt cctgccgccg
     4021 gttgccgtct atgcttcttc gctgatcaac cccgactgca aaatcattgc cgccaccaat
     4081 caaaaccgcg ccctcgcctt ccccatcggc gaattgaaaa ttatggcgaa aggcaaagga
     4141 ctgcaaatca tcggattaaa caccggtgaa tcgatgacgc ataccgccgt ttcttccgag
     4201 gcagaaatcc tgattgaaag cgaaggcagg cgcggcgcag cgcacaaaga ccgcatcccc
     4261 gtctccctgc ttgaggcaaa acgcggcaaa aaaggcagac tattacccat atcgggcagc
     4321 ctgaaacagc tttcttcccc caaataaacc cgattccaca cataattatg gtgatttcca
     4381 acccccgcga acttgaaaaa ctcaaagacc ggattcccaa tctgatcaac atcgcccgca
     4441 tcgccatcgt cctccccctg ctgattatgc atatcttcgg gttggaggtc agcgggcatg
     4501 aaaacctgta cgcctcatgg acgacatggg cattttactt gtggctcgcc atttcctgct
     4561 ggatgatttt cttttccatc atccatccgc attggcaatg gcagtcgctg aaaatgccgc
     4621 gtttcagcgc ggtggcggac atcacgatga tcggcgtgct gacctacctg ttcggcggca
     4681 tcgactcggg gttcggcatc ctgatcctgc ccttcgtggg cagctcctgc ctgctcagtt
     4741 acgggcgtta ccccctgctc tatgccagct acgccgccat cctgctgata ttcaacgcca
     4801 ttgccgacgg cgatatcggc aaatatccgc acatatcgga tgcccgaacc gcctcggcaa
     4861 ccttcatcct cgtcgccgcc tcctatcttt ccgccatctt cacctcactg tcggtcaaat
     4921 acatcgacag cgccggccaa ctggcccgcg acaaccacct cgcctaccgg cgcatcaaag
     4981 gcttgagcca aaccgtactc gaacgcgttc aagaagccgt cgtcgtcatc aacgtcgagc
     5041 atcagaccat actgttcaat aaaaaggcaa aagacctgct acccatgctt gaaatcggac
     5101 agcatactgt cctgttcgac cctattaccg ttttatggga caaggcctct tcgcgtactt
     5161 tcgaacgcaa tatcgacacg cccgccctga ccgcccgcat ccgcgccgtg ccgatgaaca
     5221 aagagcagaa caagctgctc atcctctaca tccgcccgca aagcgaaatt caggcagaag
     5281 ccctgtccgt caaacttgcc gcactcggac aactgaccgc caacctcgcc cacgaaatcc
     5341 gcaacccgat gtccgccatc cgccatgcaa acgacctgtt gcgcgaaaat atggaagcgg
     5401 gggcggcaga tccgttcaac gccaaattgt gcaaaatcat cgacggcaac gtctgccgta
     5461 tcgacaaaat gctcgaagac atttcctcgc tcaacaagcg caacaaaacc gaacgcgaaa
     5521 ccatcggcct gataccgttt tgggcggaat tcaaacaaga gttcctgctc ggccatcccg
     5581 atgccgccgg ctgcatccgt ccggacattc agggcggcag cctgaccgcc tatttcgatc
     5641 ccgcccacct gcggcaaatt atgtggaacc tcgccaacaa cgcgtggcgg cacagccgca
     5701 aacagcccgg ctcgatttcc gtcaccatcc gccccgcgca aaaaaacacc gtctgcatcc
     5761 tctttgccga ccgcccgaag tgcaggaaca cctgttcgaa cccttttaca ccacggcgga
     5821 aaacggcacc ggcctcgggc tgtatgtcgc ccgcgaactg gcacacgcca acttcggcga
     5881 tttgacctac ctgccggaag ccaaatgttt caaactcaca ttaccggaaa aaagcaatga
     5941 ctgaactgca acatcccgtc ctcgtcgttg atgacgaaac cgatattctc gacctgatgg
     6001 aaatgaccct gatgaaaatg ggcttgcgcg tccataccgc gtcaggcgtt gccgaagcaa
     6061 aaaacaagct cgacagccaa cgctattcgc tcgtcctgac cgatatgcgt atgccggacg
     6121 gctcggggct agaagtcgtc caacacatca acagccgcct gctcgatacg ccggttgctg
     6181 tcatcaccgc cttcggcaat gtcgatcagg cacaggaagc cctgcgttgc ggcgcgttcg
     6241 accccgatac catgcagata caggactatc tcgaccaaat cgaacgcgac atcatcgaac
     6301 aaaccctcaa acaaaccgaa ggcaaccgca cgcaggccgc caaacgcttg ggcatcagct
     6361 tccgttcgat gcgctaccgt atggaacgcc tcaacatcgg ctgacgacaa aacggcatcc
     6421 gcaccatctc cgcccacccg aaaaaatgcc gtctgaaacg gcacggcaaa gcgggttcgc
     6481 cccacgcccg aacggacaca aaacaccatg accgacatcc tcattgacaa caccgccacc
     6541 gaaaccgtcc gcaccctgat acaggcattc ccccttgtgc ccgtttccca accgcccgaa
     6601 caaggcagtt acctccttgc tgaacacgat accgtcagcc tcaggcttgt cggggaaaaa
     6661 agcagcgtca tcgtcgattt tgcctccggc gcggcacaat accggcgcac aaaaggcggg
     6721 ggcgaactca tcgccaaagc cgtcaaccac accgcgcacc ccaccgtttg ggacgcaacc
     6781 gcaggattgg ggcgcgacag cttcgtcctc gcctcgctcg ggctggccgt taccgccttc
     6841 gagcaacatc ccgccgtcgc ctgcctgctt tcagacggca tccgccgcgc cctcctcaat
     6901 cccgaaacgc aagacaccgc cgcgcgcatc accctgcatt tcggcaacgc cgcagtacaa
     6961 atgcccgcac ttgttcaaac acaaggcaaa cccgacatcg tctatctcga ccccatgtat
     7021 cccgaacgcc gcaaaagtgc cgccgttaaa aaagaaatga cctacttcca ccggctcgtc
     7081 ggcgaagcgc aagatgaagc ggcactcctg cataccgcac gccaaacagc aaaaaaacgc
     7141 gtcgtcgtca aacgcccccg cctcggcgaa caccttgccg gacaagcccc tgcctaccaa
     7201 tacaccggca aaagcacccg cttcgacgtt tacctgccct acgggacgga caagggataa
     7261 cgcccataaa acaagacacc gaaaaatttg ccgttcttat gcaaacgaga aaccggtttt
     7321 tgcgtttcga ctgttttgga taaatcatca caccttaaag tt
//
LOCUS       DAWEWP010000099         7348 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWEWP010000099 DAWEWP010000000
VERSION     DAWEWP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.099"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            144..1154
                     /gene="dusB"
                     /locus_tag="WGN53_001455"
     CDS             144..1154
                     /gene="dusB"
                     /locus_tag="WGN53_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ0140439.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTKKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNAQAGSALMQNEPLVAAILEAVVRAVDVPVTLKTRLGWHDDHKNLPAVARIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAAAVFKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIGEMPDGEQARREINRLDSAAAQYDMLAGYLERLAGKTDRWACGYRTN
                     AF"
     gene            1184..1423
                     /locus_tag="WGN53_001456"
     CDS             1184..1423
                     /locus_tag="WGN53_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ0140440.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            1426..1962
                     /gene="ruvC"
                     /locus_tag="WGN53_001457"
     CDS             1426..1962
                     /gene="ruvC"
                     /locus_tag="WGN53_001457"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0140441.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPPDAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            2013..2882
                     /locus_tag="WGN53_001458"
     CDS             2013..2882
                     /locus_tag="WGN53_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ0140442.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWDGKKRETVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDAQILKGRNRYDNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWESFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            3632..4426
                     /locus_tag="WGN53_001459"
     CDS             3632..4426
                     /locus_tag="WGN53_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0140443.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSTQTEDKPHWSEFAQAWK"
     gene            4638..7241
                     /gene="pepN"
                     /locus_tag="WGN53_001460"
     CDS             4638..7241
                     /gene="pepN"
                     /locus_tag="WGN53_001460"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ0140444.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSTKLLSVKINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDCFTTMGGRNVKIEFYTAEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAESRL
                     KNNIFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RTFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
BASE COUNT         1790 a         1959 c         1972 g         1627 t
ORIGIN      
        1 ccgcctgtga gtggcggggc agggaatctg tgtgcggatt atgccataaa acggtgtccg
       61 ccccaatcgc gggcgcgtcc ggagattgga aatcctgctt aaaaaatgta caatggcgca
      121 cttttttgaa acgcggatcc attatgcaca tcggcggcta ttttatcgac aaccccatcg
      181 cacttgcacc gatggcgggc attaccgaca aacccttccg ccgcctctgc cgcgatttcg
      241 gcgcgggttg ggcggtgtgc gaaatgctga ccagcgaccc gacacttaga aataccaaaa
      301 agaccctgca ccgcagcgat tttgccgatg aaggcggcat cgttgccgtg cagattgccg
      361 gcagcgatcc gcagcagatg gcggatgccg cgcgttacaa cgtcagcctt ggggcgcagg
      421 ttatcgacat caacatgggc tgtcccgcca agaaagtgtg caatgcccaa gccggcagcg
      481 cgctgatgca gaacgagccg ctggttgccg ccattctcga agccgtcgtc cgtgcggtgg
      541 acgtacccgt taccctcaaa acccgtttgg gctggcacga cgaccacaaa aacctgcctg
      601 ccgtcgcccg catcgccgaa gattgcggca ttgccgccct tgccgtccac ggacgcactc
      661 gcacgcaaat gtacaaaggt gaagcggctt acgacctgat tgccgaaacc aaatgccgtc
      721 tgaacatccc ggtctgggtc aacggcgaca tcacttcgcc gcaaaaagcc gccgccgtct
      781 tcaaacaaac cgccgccgac ggcatcatga tagggcgcgg cgcgcaaggc agaccgtggt
      841 tcttccgcga tttgaaacat tacgccgaac acggtgtttt accgcctgcc ttgagtttgg
      901 cagaatgcag tgccgccatt ttgaaccacg tccgcgccat gcacgcgttt tatggtgaga
      961 ccgccggtgt gcgcattgca cgcaaacaca taggctggta catcggcgaa atgcccgacg
     1021 gcgaacaggc gcggcgtgaa atcaaccgct tggacagtgc ggcggcgcaa tacgacatgc
     1081 ttgcaggtta tcttgaaaga cttgccggaa aaaccgaccg ttgggcgtgc ggctaccgca
     1141 caaatgcctt ctgaacactt gattatcctt tgaaagtgca atcatgcccc atacccttcc
     1201 cgatatttcc caatgtatca gacaaaattt ggaacaatat ttcaaagacc tgaacggtac
     1261 cgaaccttgc ggcgtgtacg atatggtatt gcatcaggtg gaaaagccgc tgctggtgtg
     1321 cgtgatggag caatgcggcg gcaaccagtc caaagcctcc gtcatgttgg gactgaaccg
     1381 caatactttg cgtaaaaaac tgattcaaca cggtttgctg tgaatatgtc ggcaaccgtc
     1441 cgtatcttag gtatcgaccc gggcagccgc gtaacgggtt tcggcatcat cgatgtcagg
     1501 gggcgcgatc atttttacgt cgcctccggc tgcatcaaaa cgcctcccga tgcgcctctg
     1561 gcagacagga ttgccgtgat tgtgcggcac atcggcgaag tcgttgccgt ttacaagccg
     1621 cagcaggcgg cggtggaaca ggtgttcgtc aacgtcaatc cggcatcgac gctgatgctg
     1681 ggtcaggcta ggggcgcggc attggcggca ttggtcagcc ataagctgcc cgtttcggaa
     1741 tacacggcct tgcaggtcaa acaggcggta gtcggcaaag gcaaggcggc gaaagaacag
     1801 gtgcagcata tggtggtgca gatgctgggg ctttcgggaa cgccgcaggc ggatgcggcg
     1861 gacggtcttg ccgtcgcgct gacccacgcc ttacgcaacc acgggcttgc cgccaaactc
     1921 aatccttcgg ggatgcaggt caagcgcggc aggtttcaat agtttcagac ggcatttgta
     1981 ttttgccgtc tgaaaagaaa atgtgtatcg agatgaaatt tatatttttt gtactgtatg
     2041 ttttgcagtt tctgccgttt gcgctgctgc acaaacttgc cgacctgacg ggtttgctcg
     2101 cctacctttt ggtcaaaccc cgccgccgta tcggcgaaat caatttggca aaatgctttc
     2161 ccgagtggga cggaaaaaag cgcgaaaccg tattgaagca gcatttcaaa catatggcga
     2221 aactgatgct tgaatacggc ttatattggt acgcgcctgc cgggcgtttg aaatcgctgg
     2281 tgcgttaccg caataagcat tatttggacg acgcgctggc ggcgggggaa aaagtcatca
     2341 ttctgtaccc gcacttcacc gcgttcgaga tggcggtgta cgcgcttaat caggatgtac
     2401 cgctgatcag tatgtattcc caccaaaaaa acaagatatt ggacgcacag attttgaaag
     2461 gccgcaaccg ctacgacaat gtcttcctta tcgggcgcac cgaagggctg cgcgccctcg
     2521 tcaaacagtt ccgcaaaagc agcgcgccgt ttctgtatct gcccgatcag gatttcggac
     2581 gcaacgattc ggtttttgtg gattttttcg gtattcagac ggcaacgatt accggcttga
     2641 gccgcattgc cgcgcttgca aatgcaaaag tgatacccgc catccccgtc cgcgaggcgg
     2701 acaatacggt tacattgcat ttctacccgg cttgggaatc ctttccgagt gaagatgcgc
     2761 aggccgacgc gcagcgcatg aaccgtttta tcgaggaacg cgtgcgcgaa catcccgagc
     2821 agtatttttg gctgcacaag cgtttcaaaa cccgtccgga aggcagcccc gatttttact
     2881 gactacgtaa aattacaaaa catatcaggc gtttcaagtc gaaattcctg attgtttttg
     2941 gaaatttgaa atccgggtcg tgcaaacagg atttgccgga cggttttaac ggttcagttg
     3001 tttgtaaaaa caatgctttt taagattgac aaaaaaccag aatcaggttt tagggcttat
     3061 tccgcgaaca aagggggcgg atgccgaaaa tccgattaat atattaaaat taaatttgtt
     3121 taaaaacata aagttgtaaa caagtatctc atataagcct ttttcattaa acagatagtc
     3181 agatattttg tgctaaaaat ttatataata tttaaattaa tatcaagtta taaaaatata
     3241 tagaatttta ttttgtttat ttataatttt aagcaataat ctaaatcagg cattttgatt
     3301 cccctgtttt aaaaaatact tggcaaggtg tgaaaaaagg cgtacattcc gctacacaga
     3361 attacagacg cagcggagca atgccgtctg aaaggatttt ccggtcagtc ttgcgattgg
     3421 tcggggtttc atcggaaacg gtgaaacgaa agtttgccgg cgtagagttg agctacgcgg
     3481 gcaaagccgc aggcgaaagc ctgtattgtt tgtgaagcgt aaatctctga tttgaggtat
     3541 tggggcaatc ctgtgggggg ttgcctcttt ttttatccgc cttttaatga cacaataggc
     3601 gcaagtctgt ttttgaatgc aaggtactgc catgaatacg attttggctt tcgatattga
     3661 aaccgtaccc gatgtgcagg gtatccgaac attgtacgag ctgccgtcct cgctgcccga
     3721 cgatgaagtg gtgctgtttg cccagcaaaa acgccgtgct cagacgggtg gggattttat
     3781 gcagcatcat cttcatcaga ttgtggcgat ttcgtgctgc atgcgttggg ggcaggataa
     3841 agtccatgtc ggcaccatcg gcgagatgga cgatggcgaa gaagtcgtga ttgccaaatt
     3901 tttcgagttg attgaaaaac atacgccgca actggtcagt tggaacggcg gcggtttcga
     3961 cctgccggtt ctgcattacc gctccctgat atacggcatt aacgccgcgc gctattggga
     4021 tatgggcgac ggcgatttcg gcgacagccg cgatttcaag tggaacaact acatcagccg
     4081 ttatcaccaa cgccactgcg atttgatgga tttgctcgcg ctttaccagc cgagggcaaa
     4141 cgtgccgctg gacgatatgg cgaaactgtg cggttttccg ggcaagctgg gtatggacgg
     4201 cagcaaggtg tgggatgcgt tccacgcggg caggctgaaa gaaatccgca attattgcga
     4261 aaccgatgcc gtgaatacgt atttgatgta tctgcgtttt tgtctggtca gcggcagatt
     4321 cgacgcggac gaatacgaaa tggaaatcaa gcggatcaga aactatcttt ccacccaaac
     4381 agaagataaa ccgcattggt cagaatttgc ccaagcgtgg aaatagaaaa cctgctgtcc
     4441 gaaggcggta cgcttgtgcc gccgtttcgg tttcagacgg catatggtgg attaaattca
     4501 aaccggtaca gcccgtacta tctgtactgt ctgtggcttc gtcgccttgt cctgattttt
     4561 gttaatccgc tttaaaagct gtatccgtgc tgtctgaagc cttttccagt caaaccaaac
     4621 aatattgaaa gaaaaaaatg agcaaaaccg tccgttatct gaaagattac caaacgcctg
     4681 cctaccgcat ccttgaaacc gacctgcatt ttgatattgc cgaaccgcaa actgtcgtga
     4741 aatcccgttt gacggtcgag ccgcagaggg tgggggagcc gctggtgttg gacggttcga
     4801 cgaaactctt gtccgtcaaa atcaacggcg tggcggcgga ttatgtgttg gaaggcgaga
     4861 ctctgacgat tgcagacgtg ccgtccgaac gcttcaccgt cgaagtggaa accgagatcc
     4921 tgccggcgga aaacaaatcg ctgatggggc tgtatgcttc cggtggcaat ctgtttaccc
     4981 aatgcgagcc ggagggcttc cgcaaaatca cgttctacat cgaccgtccg gatgtgatgt
     5041 ccaaattcac gaccaccatc gttgcggaca aaaaacgcta tcccgttttg ctctccaacg
     5101 gcaacaaaat cgacggcggc gagtgttcag acggccgcca ttgggtgaaa tgggaagacc
     5161 cgtttgccaa accgagctat ctgtttgctt tggtcgcggg cgatttggcg gtcacggaag
     5221 actgtttcac cacgatgggc ggcagaaacg tcaaaatcga gttttacact gccgaggcgg
     5281 acaagcctaa ggtcggcttt gccgtggaat ccttgaaaaa cgcgatgaag tgggacgaaa
     5341 cgcgcttcgg tttggaatac gacttggata ttttcatggt cgtcgccgtg ggcgatttca
     5401 atatgggtgc gatggaaaac aaggggctga acatttttaa taccaagttc gtccttgccg
     5461 acagccgtac cgccaccgat accgattttg aaggcatcga atccgtggtc ggacacgagt
     5521 atttccacaa ctggacaggc aaccgcgtta cctgccgcga ttggttccag ctttcgctga
     5581 aagaagggtt gaccgtgttc cgcgaccaag aattttccgg cgaccgcgcc agccgcgccg
     5641 tgcgccgcat cgagaacatc cgcctgctgc gccagcacca gttccccgaa gacgcaggcc
     5701 cgaccgccca tccggtgcgc cccgccagct atgaggagat gaacaatttc tacaccatga
     5761 ccgtttatga aaaaggcgcg gaagtggtgc ggatgtatca caccttgctc ggcgaagagg
     5821 gcttccaaaa aggcatgaag ctctatttcc aacgacacga cgggcaggcc gtgacctgcg
     5881 acgatttccg cgcggcgatg gcggacgcga acggcatcaa tctcgaccag ttcgccttgt
     5941 ggtacagcca ggcgggcacg cccgttttgg aagcggaaag ccgtctgaaa aacaatattt
     6001 tcgagttgac cattaaacaa accgtgccgc ccacgcccga tatggcggac aagcagccga
     6061 tgatgattcc cgtcaaaatc gggctgctga accgcaacgg cgaagcggcg gcattcgatt
     6121 atcagggcaa acgcgcgacc gaagccgtat tgctgctgac cgaagccgaa cagatcttcc
     6181 tgctcgaagg cgtaaccgaa gccgtcgttc cctcgctgct gcgcgggttc agcgcgccgg
     6241 tgcatctgaa ctatccgtac agcgacgacg acctgctgct tctgctcgcc catgacagcg
     6301 acgccttcac gcgctgggaa gccgcacaaa cgctctaccg ccgtgccgtc gccgccaacc
     6361 ttgccgcact ttcagacggt attgaactgc cgaaacacga aaaactgctt gccgccgtcg
     6421 aaaaagtcat ttcagacgac ctcttagaca acgccttcaa agccctgctt ttgggcgtgc
     6481 cgtctgaagc cgagctgtgg gacggcgcgg aaaacatcga cccgctgcgc taccatcagg
     6541 cgcgcgaagc cttgttggac acgcttgccg tccgcttcct gccgaaatgg cacgaattga
     6601 accgtcaggc ggcgaagcag gaaaaccaaa gctacgagta cagccccgaa gctgccggct
     6661 ggcgcacgct gcgcaacgtc tgccgcacct tcgtcctgcg cgccgacccc gcgcacatcg
     6721 aaaccgttgc cgaaaaatac ggcgaaatgg cgcaaaacat gacccacgaa tggggcatcc
     6781 tgtccgccgt caacggcaac gaaagcgata cgcgcaaccg cctgctggcg cagtttgccg
     6841 acaagttttc agacgacgcg ctggtgatgg acaaatattt cgcccttatc ggctcaagcc
     6901 gccgcagcga caccctgcaa caggttcaaa ccgccttgca gcatccgaaa ttcagcctcg
     6961 aaaaccccaa caaagcccgt tcgctcatcg gcagcttcag ccgcaacgtc ccgcatttcc
     7021 acgcagaaga cggcagcggc taccgcttca tcgccgacaa agtcatcgaa atcgaccgct
     7081 tcaacccgca ggtcgccgcc cgtttggtgc aggcgttcaa cctctgcaac aagctcgagc
     7141 cgcaccgcaa aaacttggtg aaacaagcat tgcagcgcat tcgggcgcag gaaggattgt
     7201 cgaaagacgt gggcgaaatc gtcggcaaaa ttttggattg aggccgtcaa acagaaaaac
     7261 aatgccgtct gatatagtta cacacacgtt ttatcatcac ttccccatcg ttttattacg
     7321 caatggcaaa acggccgcaa agtcatat
//
LOCUS       DAWEWP010000100         7332 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWEWP010000100 DAWEWP010000000
VERSION     DAWEWP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.100"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            198..410
                     /locus_tag="WGN53_001461"
     CDS             198..410
                     /locus_tag="WGN53_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ0140445.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            558..2141
                     /locus_tag="WGN53_001462"
     CDS             558..2141
                     /locus_tag="WGN53_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ0140446.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     KHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEAFKTEDVAENLARRFPGLTF
                     DRMKINSQLLLQTILGRFSVSSDNAGKQFFEDHKTYVPARFTDYVAAFVEHGAEAFVQ
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2223..3320
                     /locus_tag="WGN53_001463"
     CDS             2223..3320
                     /locus_tag="WGN53_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ0140447.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIGVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3434..4549
                     /locus_tag="WGN53_001464"
     CDS             3434..4549
                     /locus_tag="WGN53_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ0140448.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNLASVSTENRYKIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4674..5129
                     /locus_tag="WGN53_001465"
     CDS             4674..5129
                     /locus_tag="WGN53_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ0140449.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5200..5676
                     /locus_tag="WGN53_001466"
     CDS             5200..5676
                     /locus_tag="WGN53_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ0140450.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5664..6590
                     /locus_tag="WGN53_001467"
     CDS             5664..6590
                     /locus_tag="WGN53_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0140451.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6655..7212
                     /locus_tag="WGN53_001468"
     CDS             6655..7212
                     /locus_tag="WGN53_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0140452.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGFTEDLVQKATDKAVANESGNGLKN
                     TAGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATHGLYQNVPVQPVKIRRIVVGQ"
BASE COUNT         1925 a         1801 c         1905 g         1701 t
ORIGIN      
        1 cgccgattgc caaggcgatg atgctcaacc agggtttttg tgaagcgttc attgttgtcg
       61 tcctgatggg aattgccgcc attatgcgca agcgtccgtc tttttccaaa ccgccgtacc
      121 gtttccgcgt ttgcccaagt tcacgccccg acttacaata acgcccgtca gattgcagtt
      181 ccaacgaaag gaaaatgatg gaaaccctta tcctcgacat cggcggcatg agctgcggcg
      241 gctgcgtcaa aagcgttacc cggatattgg aaggcgtaaa aggcgtggca agcgtcgaag
      301 tcagccttga aaacaaaagt gcgaccgtcg gatacgaccc cgcgcaaacc gatgcagggg
      361 cgttgattga agccgttgaa gacggcggct atgatgccgc gttgaaataa agcggcaaaa
      421 atgccgtctg aagccttcgc gcctccccga tgccgtctga agtacgctcc gcgccttcag
      481 atggcatttg atttagttga acgggtagga tgaattggtt aaacttgcgg gcaatagtgc
      541 cttcagacaa acaaaatatg ccgaacaaaa agaaaaaagc cgcctttgcg cgtgctgcaa
      601 cggagaacgg tgtgccggac gtgtccaaca tcaaaaattc atatgatgat ttgatgtatg
      661 aatccaaagc attttcccaa accgccatca acgccttgga ggctcgcgcc cgcatgatgg
      721 gtttgcagcc cgcgcccgcc gccaatgcca aggtcttgga gctgggctgt tcgatgggcg
      781 gcaacatcat cacgcaggct ctttattacc cggatgcgga atttgtcggt atcgacttgt
      841 ccggcaggca ggttgcgcag ggcaacgcca tcattgaaaa aatgggcttg aaaaatgtgc
      901 gtctggaaga aaaagatatt ttgaccgtcg atgagtcatt cgggaagttc gactatatca
      961 tcgtccacgg catttggtcg tgggtgcctg acgcagttaa agacaaaatc ttttcgattt
     1021 gccggaacaa cctgaccaaa cacggcattg cctatatttc atacaatgtt taccctggct
     1081 ggaaacggca ggagcagttg cgcgaaatta tgtactttgc cggcagggat gtgcttgaag
     1141 aacccttgga agcgcggacg cggaaaggct tggacgcgct caaggcgctg gcggaaattt
     1201 tggaaaacga caaaggcttg ggcggcggcg gcaaacttcc ggcgattcaa aaaatattga
     1261 atcacaatac ttattatgtc gcacacgaat atatggaaat attcaacgac ccgatttacg
     1321 ttaacggctt catcgaatgg gcaaaccgcc accggctggc gtatatcggg gataccgatt
     1381 tgcacgcgtc ttttgtttct tggatggcgg agcatacgcg ggagcggatt ctggcattgg
     1441 cagggggcga ttatattgcc aaagaatttt acagcgatat tttatccgac cgccaattcc
     1501 gccgttcgct tttatgccgt gaggaagtcg gggatactgt caggcgtgac gagtcggttg
     1561 ccgtcgaagt gatagaaagt ttgaatttcc gcccggcaag aggggaaaca atcaactttg
     1621 atgaaaacga taccctgctt tcgggcatac gcgatgtgat gaaaaccgga gaggcgttta
     1681 aaacggaaga tgttgcggaa aatctcgccc gccgatttcc cggtttgaca ttcgaccgta
     1741 tgaaaatcaa ttcccagctt ttattgcaaa ccattctcgg gcgtttttct gtttcatcag
     1801 acaatgcggg caaacaattt tttgaagacc ataaaaccta tgtgccggcg cgttttacag
     1861 attatgtcgc cgcctttgtg gaacacggcg cggaagcgtt tgtccagcct gccaaccgtt
     1921 acaacgaaag caccccctca ttcgggtacg ggcatttgta cattatgcgg caattgtcgc
     1981 ggccgacgag caaacaggca ttgattgaaa cggttgccga aaacttgaac atcgtcagca
     2041 ccacgcccga cggtttgaca ttccatccgc ctgccgaagt gtatgtggaa gaaatattgg
     2101 cagacttggc agacaggcat tttctcgttt cggcggattg acgggcaagt cccgacggca
     2161 atccgtccga tatattttca aacaggaaaa acacacactg cgaaaagcgg tacacgacat
     2221 atatgaacca gacatttacc ttgcccgata cgcgcccgta tccccaaaat ccgattaaaa
     2281 accacctgct gctcaatgcc taccagttgg cgcataattc ttcccaggct tcgcgcaaac
     2341 tctcgtccgg ccaacttcaa accgaaatca gggggatgct tgagcaaaac cactatatca
     2401 acctttccct cgcgctgacg atgtcgcccg atgccggaac ttatgccgcg ctgctttcca
     2461 gtgtgaacgc ggtactcgat tgcgagaaag aaggcgaagt gcagtggttc gccctgccgg
     2521 tcgtgctggt gtccggctgc aagaaagaac gggcaatcga gatgaagctg ccgacggagg
     2581 gattgtttgc ctgtttgcaa aactatccgc acctgcgcgc gttgacgcaa gagacgcaat
     2641 ggctgcctta tcttgtacat tcctccgatt tgagtgcggt cgcgccggat gagtggtggc
     2701 gtgccaaaca aaataccgaa gcggcggcgc aacacttacg ccgtttcgcc ccgcgccctt
     2761 tgcttttgcc cgaagggcag tccgtccacg tcgtttacgc gctgggtttc ggcagcggca
     2821 aagttcagac ggcattgggt cagaaccttc ttcaggcagg tctgcccttg atgcaggtat
     2881 ggcaggaaaa tcttgcatcg gaaggcgtta cgctgtttgc caatccgctt tccccggatt
     2941 ctccggtacg cgcgctttcc gacggcagcc acacgcgcca gcgcatggcg atggatgtgt
     3001 ttgcggcaaa cgcgatacgc gccgtccgta tgcagagtcc gcgcgtcggc gtggtcgcag
     3061 cggcaaaggc gggcggacag attttattcg ggtttaatgc gaccgacggc gcgtttgaag
     3121 tcgtgccgca ggtgttttcg tggcagcttt cctttaccga caatattggc gttattcagc
     3181 aaaatttttt ggatttaatg gcagaatgcc gtgtagaaca cgtttacctg ttgcacaatc
     3241 ccttgggcga acaggaaagc atcccgagct atgcggaagc attgaaacgg gaagggcaca
     3301 atccgttttt cagcgcatag tgatttgtag tagtgatagg ctttctcatt tctataataa
     3361 atcaaaataa agagcacaac actttttcat tctgtgttgt gccttgagtg aaacgaaagg
     3421 atgaattata aatatgaaga ttgtaattgc accggattcg tttaaggaaa gcttgacagc
     3481 tcaacaggta gctgaagcaa taaaaagagg cttccaacaa tcgatagcag atgtggaatg
     3541 tctcctctgt cctgtcggtg atgggggaga aggcactgta gatgctatcc gacattctct
     3601 tgacctagaa gaaaaatgtc tccaagtgac aggacctttt ggacaaaaag aagtcatgcg
     3661 ctattttcaa aaagaacaac tagccctatt cgaagttgct gatttggttg gtcttggaaa
     3721 aatcccgcta gagaaacgaa atccattaca aatccaaact cgtggtattg gagaattgat
     3781 tcgccacctc attagtcaag aaattaaaga aatctatatt ggcgttggcg gtacggccag
     3841 taatgatgga ggtattggga ttgctgctgg tttaggttat caattttatg atgaggatgg
     3901 aaatgcctta cccgcttgcg gtcaatcctt actaaaccta gcttctgttt caacagaaaa
     3961 tcgctataaa attcctgaag atgttcacat tcgtatttta gcagatgttg tgagtccctt
     4021 atgtggacac caaggtgcga cctatacgtt tggcaagcaa aaagggttgg attctactat
     4081 gtttgaggcc gtagatcagg caatacaaga tttttatgaa aaagtctccc ctgcaacatt
     4141 aaaacttaaa ggagcaggag ctggtggagg catcgctggt ggtttgtgcg cctttgctca
     4201 ggcaagtatc gtatctggaa ttgacacctg cctggacttg attgactttg ataagaaagt
     4261 gtcagatgtt gacttggtta tcgttggtga aggaagacta gatcgtcaaa gtttagcagg
     4321 gaaagcgcct attggtgtag caaaaagaac ccctgtcgga gttcctgttg tcgctatttg
     4381 tggcagtctc gttgaagatt tgccttccct gccatttgaa aatatccaag ctgccttttc
     4441 tattttggag aaaagtgaac ctttagaaga tagtttgaaa aatgccagtc tctatttgga
     4501 gcatacggct tctaatattg gacacttatt aaatatgcct aagatttagc caaaccattt
     4561 cttccagatg gatgttttgg ctggttctgc cttatgcggt tacgattaac cgaccgatga
     4621 cgtctgaaag ggtttccgct tcagacggca tcgcgccgga acagggaggg gatatgaaaa
     4681 aaccgaaaat cctttttgtc tgcctcggca acatctgccg ttcgccgatg gcggaataca
     4741 ttttgcgccg ccgcgccgcc gaagcgggca ttccccttga agcggacagc gcggggacat
     4801 cgggctggca cgacggcgaa gatatgcacc gcgagacggc aaagatattg aaaaaacacg
     4861 gtatcgatgc ttcaggcttt accagccgca aaatccgcca aagcgatgcg gcggcgtttg
     4921 actgcatcat cgcgatggac ggcaagaatt tgtccgaatt ggaaagaacc ttcggcaggc
     4981 ggccggaaaa aatattcaag ctgaccgacc tgatacccga aagcggttac gaccatgtcc
     5041 ccgatccgtg gtatacgggc gattttgaag aaaccttcag gcttgcggat gcgggctgtc
     5101 gggcattgtt ggaaaagatt tccaaataaa gcaattgaat acaaatataa aaccgcctcc
     5161 tgccgtatcg gttgttcaga cggcataaac agagaattta tgaaaacaaa tttcaaacag
     5221 aaaattatcg aacaggcacg cagcgaaggc ttgcaggtaa ccgctttgcg cgagcaggta
     5281 ttagatattg tcttgcagca aagcggcgtg attaaagcct acaacgtctt gtcgcagatg
     5341 cagcagcaaa gcgagggcgt gcttgcaccg cctaccgcct accgcgccct tgatttttgg
     5401 gcggagcagg gcgttttgca caaagtggcg gcggtcaacg gctatatttt gtgcagccac
     5461 gcgcagcacg agtgcgacga ccattgccac gaccacgaag aagccgaagc gcaccacagc
     5521 gcgtttattt tggtctgcac cgaatgcggc acggcggacg agcaaaccct cagccacgag
     5581 tgggcggcac tgcgcgcagg cgttgccgaa agcggctttg cgctgaaaga agaacacgtt
     5641 gttttaactg gaatctgtaa aaaatgtcag aaatgaaaaa aaccaaagtc cacctgattt
     5701 caggttttct gggaacaggc aaaaccactg cgctcaaaag cctgatggcg caaaaagacc
     5761 cgaacgaaaa atgggtcatc atcgtcaacg agttcggcga aatcggcatt gacggcgcgg
     5821 tattgagcga caacggtatc cctgtggcag aaatcgccgg cggctgtttg tgttgcaccg
     5881 ccggcccgca aatgggcgta accgtgcaga aaatgctgcg cgacgccaag cccgaccgcc
     5941 tgatgattga agcaagcgga ctggctcacg cagccagcgt catcgacgaa ctcaaagcca
     6001 aaccgctgga cagccttttg gaaatcggcg ccgtctttac cgtcgtcaat ccgcgccagt
     6061 tcatcaaccc cgattacgcg caacaggcgt tgtataaaga ccaaatcggc atctgcgacg
     6121 tattggtcgc cagcaaaacc gatttatgca cccccgaaca gcttgccgaa tttcacgaca
     6181 aagcggcaaa actgttcccg cccaaagcca aagtggttga agtccaaaac gcacaactcg
     6241 acatccggtg gctcgatatt cccgtcatcg aaaaatcacg ctaccgcctc aaagccctgc
     6301 cggataatac catgggcttc cagtcgcaag gtttcacatt ccccgccgga cgcgatttcg
     6361 acggcgaaaa gctgaccaac tttttcaacg acctgcccaa aatgaccgaa ggactcgtcc
     6421 gcgccaaagg cgtgtttcaa gtattgggaa cgtgggtgtg gctcaactgg gtagacggac
     6481 agtggggcgc gaaccaagtc tcatggcggc gcgattcgcg ttttgaattg attgccaaat
     6541 cgtttgatgc ggatttaatc gaacaaaagc tcaaagacgc attggaatag cgttcacgcg
     6601 ccttcagacg gcatatgaaa tgaaaatgcc gtctgaaggt ctgaggagga cgggatgaaa
     6661 cccaaattca aaaccgtttt aaccgcgctg cttttggcgg tttccctgcc gtctatggcg
     6721 gcaacccatg ttttgatgga aaccgatatg ggcaatatcc gtttggtttt ggacgagtcc
     6781 aaagccccca aaaccgttgc taatttcgtg cgctatgccc gaaaaggctt ttacgacgac
     6841 accgtttttc accgcgttat cgacggtttc gttatccagg gcggcggatt taccgaagac
     6901 ttggtacaaa aggcaaccga taaggccgtt gccaacgaat ccggtaacgg cttgaaaaac
     6961 actgccggta ccatcgccat ggcgcggacg gccgatccgg attccgccac cagccaattc
     7021 tttatcaatc tggcggacaa tgattcactc aactacaaaa acggacaata cggctatacc
     7081 gttttcggca gggtcgaaag cggcatgaac accgtttcca aaatcgcccg cgtcaaaacc
     7141 gccacgcacg gcctttatca aaacgtaccc gtacagcccg tcaaaatccg tcgcattgtt
     7201 gtcggacagt aacacgcaga cagatgttca gacggcgtcg cccgtttcac aaaaaacgcc
     7261 gtctgaagtg aaaaaatatt ttaaaacaga tagttgatat tgacaaattc aaaccgagga
     7321 ttttaaaatg ct
//
LOCUS       DAWEWP010000101         7204 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWEWP010000101 DAWEWP010000000
VERSION     DAWEWP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.101"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(64..148)
                     /locus_tag="WGN53_001469"
     tRNA            complement(64..148)
                     /locus_tag="WGN53_001469"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Leu,seq:caa)
     gene            425..1078
                     /locus_tag="WGN53_001470"
     CDS             425..1078
                     /locus_tag="WGN53_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ0140453.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1157..1810
                     /locus_tag="WGN53_001471"
     CDS             1157..1810
                     /locus_tag="WGN53_001471"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ0140454.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1914..2813
                     /locus_tag="WGN53_001472"
     CDS             1914..2813
                     /locus_tag="WGN53_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ0140455.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2810..4099
                     /gene="hisD"
                     /locus_tag="WGN53_001473"
     CDS             2810..4099
                     /gene="hisD"
                     /locus_tag="WGN53_001473"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ0140456.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4145..5242
                     /locus_tag="WGN53_001474"
     CDS             4145..5242
                     /locus_tag="WGN53_001474"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ0140457.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5239..6156
                     /gene="hisB"
                     /locus_tag="WGN53_001475"
     CDS             5239..6156
                     /gene="hisB"
                     /locus_tag="WGN53_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ0140458.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6251..7120
                     /locus_tag="WGN53_001476"
     CDS             6251..7120
                     /locus_tag="WGN53_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0140459.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1880 a         2123 c         1723 g         1478 t
ORIGIN      
        1 ttagttttat ctatttcatt aaacagcaat agacaaaaaa aataaccgct ctaaaagcgg
       61 ttgtggtgcc cagggtcgga ctcgaaccga cacaccttgc ggcgggggat tttgagtccc
      121 ctgcgtctac caatttcgcc acctgggctg gtgaagaagt cgtcattata atggcttttg
      181 aaattctgta aacctttttt ttgaaattat tttatctgtt tttattttat ttttgatttt
      241 aaatagaatt tttattattt taatcttact gttctttccg ctccaaagat tctgtatgat
      301 tcggcaattc ctgccgtgca gacaacgtaa aaaaatacta cattaaatct gccaaacgcg
      361 ttaagatgga aatattcaaa ttccgtacga atcaggtttt gctatttatt cttgggagat
      421 tgtcatgttt tccgtaccgc gttccttttt gccgggcgtt ttcgtacttg ccgcgcttgc
      481 cgcctgcaaa cctcaagaca acagtgcggc gcaagtcgct tcttcaagtg catccgcgtc
      541 ggctgcggaa aatgcggcaa agccgcaaac gcgcggtacg gatatgcgta aggaagacat
      601 cggcggcgat ttcacgctga ccgacggcga aggcaagcct ttcagcctga gcgatttgaa
      661 aggcaaggtc gtgattctgt ctttcggctt tacgcactgt cccgatgtct gcccgacaga
      721 gcttttgacg tacagcgaca cgttgaagca gttgggcggg caggctaagg acgtgaaagt
      781 ggtgttcgtc agcatcgatc cggaacgcga cacgcctgaa atcatcggca agtatgccaa
      841 acagttcaat ccggacttta tcggtctgac ggcaacgggc gaccaaaacc tgccggtcat
      901 caagcagcaa taccgcgtgg tttctgccaa agtcaatcaa aaagacgaca gcgaaaacta
      961 tttggtcgac cactcttccg gtgcgtatct catcgacaaa aacggtgagg ttgccatttt
     1021 ctcgccttac ggaagcgagc cggaaacgat tgctgccgat gtaaggactc tgctctgata
     1081 aaaccgtatg ccgtctgcac cgtcggcgcc tattcagacg gcattattgt ttcaaccgac
     1141 aaaggacatc cacaccatgc aggataatgc tttgacaatc gccttatcca aggggcgcat
     1201 ttttgaggag acgctgccgc tgcttgccgc tgccggcatt gttccgactg aagagcctga
     1261 aaaatcgcgc aagctgatta tcgggacgaa ccatgaaaac atccgccttg tcattgtccg
     1321 cgcaaccgat gtgccgactt atgtccgcta cggcgcggcg gacttcggca ttgcgggcaa
     1381 agacgtgctg atcgaacacg gcggcacggg gctttaccgg cctttggatt tggagattgc
     1441 caagtgccgc atgatggttg ctgtgcgtaa agggtttgat tacgaagcag cttcgcaacc
     1501 cggatgccgt ctgaagattg ccacgaagta tcctgaaatc gcggcatctc attttgccgg
     1561 caagggtgtc catgtggaca ttatcaaact gtacggctcg atggaacttg cgccgctggt
     1621 cggcttgagc gatgcgattg tggacttggt ttcgacgggc aacaccttga aggcaaacgg
     1681 cttggaagca gtcgaacaca tcgtcgacat ttccagccgc ctggtggtca acaaggctgc
     1741 tttgaaaacg aaatacgcgc tgctggagcc gattattcag gcgttcggcg gcgcagtgaa
     1801 ggcgaagtaa gcatccattt gaataaagat gcgttttcag acgaccctat ccgttcccgc
     1861 cgacaggtcg tctgaaaata tcaccggcag taaactgtat aggagaagtt aaaatggttg
     1921 caaaaataaa aaaattctca gattcaaccc tttccgtttt gaataacggc gagcgtcggt
     1981 tttatgtcta ttgtctgacc gacctgaaaa aagacaaaat cctctacatc ggcaaaggct
     2041 gcggtaatcg tatcttcgag catgaatggg ttgctagtcg ttcacaagac ccagtctccg
     2101 gcgagattat cgatcggaaa ctcaaagcca tctccaaatg caagaaactc ggtcgctata
     2161 tcatcagcta tcatctgact gaagtcgaag cactcgccgc cgaatctgcc ttaattcatt
     2221 ttgttaaatc tgtcttgggt aaaaaactca aaaataaaat tgccgggcat ggtccgggtg
     2281 gtattagcgt agaagaacta gatcgccgct ttggattctc ttctctccca cttaacgaga
     2341 ttaaccccga cgggctgatt ctcgccatca aaatccacaa tgctttcgat ttagatactg
     2401 acgaagaatt agactacctt ttcgacaacc aagacgatgc caacctcaaa tcgcgtacgt
     2461 tgggcaactg ggttatcggt aaagatgttg cttcaaaagt gaaatacgtt atcggcgttc
     2521 acaccggtct gcaaaacgct gttgtcagtg catacgaagt ggacggtttt gaaacaatgg
     2581 ttgaggaaac caaaaacggt agaaaacaat cccgttaccg tttccgcact acctctcgta
     2641 gcgaagaggt attagccaaa ctcggtctgc aacaaaaatg cctgcccgaa ttgaagtttg
     2701 gtagcggggg agaaaaagcg tatatcagac ccaaaacaga gacagaaact gaacaagaga
     2761 atattcagac gacccccaat ccaaaaataa aaaaggaaaa aaccaaatca tgaaaaaact
     2821 caacacccaa tcgcccgatt tccaagccgg actcaaagcc ctgctggctt ttgaaaccgc
     2881 gcaaaacccc gaaaccgaac gcatcgtcgc cgacatttgc gccgacgtgc aaaagcgcgg
     2941 cgatgcggct ttgattgaat acaccaacaa attcgatcag acaaacgcta aaagcatcga
     3001 tgatttaata ctcacgcaag ccgatttgaa cgcggcgttc gagcgcattc cgaacgacgt
     3061 tcagacggca ttgcagaccg ccgcccgccg tgtcgaaagc taccaccaac gccaaaaaat
     3121 ggaatcgtgg agctacaccg atgaagacgg cacgctgttg ggacaacaga ttaccccgct
     3181 tgaccgcgtc ggcatttacg tccccggcgg caaggcggca tatccgagtt ccgtcatcat
     3241 gaacgccatg cccgcccacg tcgcaggcgt gaaagaaatc atcatggtcg tgccgacacc
     3301 gaaaggcgaa cgcaacgaca tcgtgcttgc cgccgcatac gtcgccggcg taaccaaagt
     3361 cttcaccgtc ggcggcgcgc aggcggttgc cgccctcgcc tacggcacgg aaaccatccc
     3421 ccaagtcgat aaaatcaccg gtccgggcaa cgccttcgtc gccgccgcca aacgccgcgt
     3481 gttcggcgtg gtcggcatcg acatggtggc ggggccgtct gaaatcctgg tcatcgccga
     3541 cggcacgaca cctgccgatt gggtggcgat ggatttgttc agccaggccg aacacgacga
     3601 aattgcccaa gccatcctca tcggcacgtc gcaagcgtat ctcgacgaag tagaagccgc
     3661 tatggaccgc ctgatcgaaa ctatgccgcg ccgcgacatc atcgaagcct cgctcggcaa
     3721 caggggcgcg atgatactcg ccaaagactt ggacgaagcc tgcgaaatcg ccaactacat
     3781 ttcccccgaa cacttggaac tgtcagtcga aaatccgcag gaatgggcga aaaaaatccg
     3841 ccacgccggt gcgattttca tgggacgcta caccggcgaa agcctcggcg actactgcgc
     3901 cggtccaaac catgtattgc ccaccagccg aaccgcccgc ttttcctcgc ctttggggac
     3961 atatgatttc caaaaacgct ccagcctgat tcaggtttcg gaacagggcg cgcaaaaatt
     4021 aggcgaaacc gccagcgtgc tggcacacgg cgaaagcctg accgcccacg cccgcgcggc
     4081 agagttccgt atgaaataat gccgaaacgg cgtacaggca tattccaacc attaaggaaa
     4141 cacgatgaaa tccgtccgct ccttcatccg cgacgacata caagctatgt cggcatatca
     4201 gattgccgac gttccgcccg gctttgccaa actcgattcg atggaaagtc ccgtccaccc
     4261 ttttgccgga catgaaacgc tgttgcagga atggcaggca cggcttgccg ccgcgcccat
     4321 ccatctttac cccaatccct ccggcagcgg tttacaggaa gcattacgtt cggcgttcga
     4381 cattcccgac tgcgccgaca tcgcgctggg caacggctcg gacgagctga tacagttcat
     4441 cacgatgctg accgccaaac cgggcgcggc aatgttggca gccgaaccca gtttcgtcat
     4501 gtaccgccac aacgccgcgc tgtacggcat ggattatgtc ggcgttccac tgaacggaga
     4561 tttcaccctc aacctgcccg ccgtcctcga agccgtcagg aagcaccgcc ctgccctgac
     4621 ctttatcgcc taccccaaca accccaccgg cgtatgtttc acgcgtgccg aaatcgaagc
     4681 cgtcatcgaa gcttcagacg gcatcgtcgt cgtcgacgaa gcctacggcg cattcaacgg
     4741 cgacagcttc ctgccgcagg cgggcagcat tcccaacctc atcgtcatgc gtaccgtcag
     4801 caaaatcggt tttgccggac tgcgtatcgg ctatgcggca ggctgtcccg aagtcatcgg
     4861 cgaactgcaa aaaatcctgc cgccctacaa tatgaaccaa ctgagcctga ccaccgccaa
     4921 actcgccctg caacactacg gcatcatctc tgccaacatc gacagcctga aaaacgaacg
     4981 cgaacggatg ttcgccgaat tgggcaaaat atgccgtctg aacacctttc caagtcaggc
     5041 aaacttcatt accatacgcg tacccgatgc cgatttgttg tttgacacgc tcaaacaaaa
     5101 ccgcatcttg gttaaaaaac tgcatggcgc gcacccgctt ttggaacact gcctgcgcat
     5161 taccgtaggc agccccgcac aaaacgatgc cgttctcaac atcattcgcc aactttactg
     5221 ccaaccaacg gatttcctat gaatttgact aaaacacaac gccaactgca caactttctg
     5281 accctcgccc aagaagcagg ttcgctgtcc aagctcgcca aactctgcgg ctaccgtacc
     5341 cccgtcgcac tctacaaact caaacaacgc cttgaaaagc aggcagaaga cccagatgca
     5401 cgcggcatcc gtcccagcct gatggcaaaa ctcgaaaaac acaccggcaa acccaaaggc
     5461 tggctcgaca gaaaacaccg cgaacgcact gtccccgaaa ccgccgcaga aagcaccgga
     5521 actgccgaaa cccaaattgc cgaaaccgca tctgctgccg gctgccgcag cgttaccgtc
     5581 aaccgcaata cctgcgaaac ccaaatcacc gtctccatca acctcgacgg cagcggcaaa
     5641 agcaggctgg ataccggcgt acccttcctc gaacacatga tcgatcaaat cgcccgccac
     5701 ggcatgattg acatcgacat cagctgcaaa ggcgacctgc acatcgacga ccaccacacc
     5761 gccgaagaca tcggcatcac actcggacaa gcaatccggc aggcactcgg cgacaaaaaa
     5821 ggcatccgcc gttacggaca ttcctacgtc ccgctcgacg aagccctcag ccgcgtcgtc
     5881 atcgaccttt ccggccgccc cggactcgtg tacaacatcg aatttacccg cgcactaatc
     5941 ggacgtttcg atgtcgattt gtttgaagaa tttttccacg gcatcgtcaa ccacagtatg
     6001 atgaccctgc acatcgacaa cctcagcggc aaaaacgccc accatcaggc ggaaaccgta
     6061 ttcaaagcct tcgggcgcgc cctgcgtatg gcagtcgaac acgacccgcg catggcagga
     6121 cagaccccct cgaccaaagg cacgctgacc gcataaaaaa ccataccgtc tgaaacaccc
     6181 gcaggctttt cagacggtat cggaacagat aagattacac tacactacaa acagaaaagg
     6241 agtaaacatc atgtccgcaa acgaatacac acaaatcggc tggataggct tagggcaaat
     6301 gggtctgcct atggtaacgc ggctcttgga cggcggcatc gaagtcggcg tatacaaccg
     6361 ctcgcccgac aaaactgccc ccatctccgc caaaggcgca aaagtttacg gcaacaccgc
     6421 cgaactcgtc cgcgactatc ccgtcatttt cctgatggtt tccgactatg ccgccgtgtg
     6481 cgacatcctg aacggagtcc gcgacggatt ggccggcaaa atcatcgtca acatgagcac
     6541 catctccccg accgaaaacc tcgccgtcaa agcacttgtc gaagccgcag gcggacagtt
     6601 tgccgaagca cccgtttccg gatcggtcgg gcccgccacc aacggcacgc tgctgattct
     6661 gttcggcggc agcgaagccg ttttaaaccc gctgcaaaaa atattttccc tcgtcggcaa
     6721 aaaaaccttc catttcggcg atgtcggcaa aggttcgggc gcgaaactcg tcttgaactc
     6781 gctcttgggc attttcggcg aagcgtacag cgaagcgatg ctgatggcgc ggcagttcgg
     6841 catcgatacc gacaccatcg tcgaagccat cggcggctcg gcaatggact cgcccatgtt
     6901 ccaaaccaaa aaatccctgt gggcaaaccg cgaattccca cccgccttcg ccctcaaaca
     6961 cgcctccaaa gacctcaacc tcgccgtcaa agagcttgaa caggcaggca acaccctgcc
     7021 cgccgtcgaa accgttgctg ccagctaccg caaagcagtc gaagccggct acggcgaaca
     7081 ggacgtttcc ggcgtttacc tgaaattggc agaacactga ttgccttttc caaacacaat
     7141 gccgtctgaa catatttcag acggcatttt tatcacccca cgcttaaaat cagtcccgat
     7201 tatg
//
LOCUS       DAWEWP010000102         7092 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWEWP010000102 DAWEWP010000000
VERSION     DAWEWP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.102"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..138
                     /locus_tag="WGN53_001477"
     CDS             <1..138
                     /locus_tag="WGN53_001477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0140460.1"
                     /translation="LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVATARR
                     N"
     gene            138..749
                     /locus_tag="WGN53_001478"
     CDS             138..749
                     /locus_tag="WGN53_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0140461.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLKIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVPSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            746..1306
                     /locus_tag="WGN53_001479"
     CDS             746..1306
                     /locus_tag="WGN53_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ0140462.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            1317..3290
                     /locus_tag="WGN53_001480"
     CDS             1317..3290
                     /locus_tag="WGN53_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ0140463.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLSTAVGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGMTAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDTA
                     AVGYEDHNERQRSALVSTWTDGDLNDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(3969..4280)
                     /locus_tag="WGN53_001481"
     CDS             complement(3969..4280)
                     /locus_tag="WGN53_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HEZ0140464.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMQTGAR
                     A"
     gene            complement(4288..4566)
                     /locus_tag="WGN53_001482"
     CDS             complement(4288..4566)
                     /locus_tag="WGN53_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0140465.1"
                     /translation="MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPS
                     DMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFNDGNAEVVDYQDYH"
     gene            4651..5256
                     /locus_tag="WGN53_001483"
     CDS             4651..5256
                     /locus_tag="WGN53_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140466.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQTQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            5235..5531
                     /locus_tag="WGN53_001484"
     CDS             5235..5531
                     /locus_tag="WGN53_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ0140467.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            5528..5737
                     /locus_tag="WGN53_001485"
     CDS             5528..5737
                     /locus_tag="WGN53_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140468.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(5847..6990)
                     /locus_tag="WGN53_001486"
                     /pseudo
     CDS             complement(5847..6990)
                     /locus_tag="WGN53_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         1787 a         2077 c         1934 g         1294 t
ORIGIN      
        1 ctgcaagtgc gtatcggcca gcgtttttac cgcactaccg cccgcgccgt tatcggcagc
       61 ggcggcacgg cggaaatacc ggcaatcgcc gacgagccgg gcgcggccgc caatgtggcg
      121 acggcgaggc gcaactgatg gccgcccccg ccggtgtggc caccgaatgc cgccttaccg
      181 tacaaggcgg caccgaccga gaaagcgatg cctcactgct ggcgcgtctg ttgaaaatca
      241 tccgccgacc gcccgcaggc ggcaaccgtt acgactataa aaactgggcg ttgagtgttg
      301 acggcgtaac cagcgcatat gtttatccgc tgcgccgcgg cttgggtacg gtggatattg
      361 ccattacctc cgccgacggt gtgccatcgg aagaaactgt gcgccgcgta caggcttata
      421 tcgacgagat gcgcccggta acggcaaaaa atgcgctggt actcaagcca accgtaacgg
      481 cggtgcctgt taccgtgcaa gtcaagctcg acggcatcga cttggacgag gccaagcgcc
      541 gcatacggac ggccctaaaa gaatatttcg acaccctgat ccccggcgac ggcctgactg
      601 tgtcgcaaat cgaggcggct atcagcaatg tggatggtgt gatcgaccgc cgtctgactg
      661 cgccgacggc caaccgtgcc gccgatacgg ttaaccgcat cgagtggttt aaagcgggcg
      721 cgattaatgt aacggagatg ccgtcatgag ctatcaagac atcttgcggg gtctgttgcc
      781 ccccgtgtcg tatgcccgca atgccccgcg tgtgcgggcg caggcagaaa tagacggcgc
      841 agcgctggat gcggtagcgg aatcggctca aagcgttgcc gatgccgtcg acccgcgcag
      901 cgccggccaa atgctggccg attgggagcg cgtattaggt ttggacggta cgggcaaaaa
      961 ccgccagcgc cgtgtgttgg ccgtcatggc caagctaaac gaaacaggcg gcttgagtat
     1021 tccttatttt gtgcgtttgg ccgaggcggc gggctatcaa atccaaatcg acgaaccgca
     1081 gccgttccgc gccggtgtca accgtgcggg cgaccgtctt gcgccgcagg aaatcatgtg
     1141 ggtgtggcac gttaacgtgc gcggcggcaa caaccgcatt acccgattcc gcgccggtat
     1201 ctcggcggcg ggcgacaggc tgaccgatta cagcgatgcc gtgatcgaaa gcctgttcaa
     1261 ccgcctcaag cccgcccaca ccgctatccg atttacctac cgataaggac taaaccatgc
     1321 accccatcga aacccccgat aagaccttcc acgacggcga cggcgtgtcc gaattgggca
     1381 ccatcctgcc cgcgtggtgg ctcaaccaag tgcaatccga gctgctggcc gtgctgactg
     1441 cggccggtat ccagccggat aagtcgcagc ctaatcaatt actggcagca ctgaatagac
     1501 tggctgtagt taccaccggc aaccaagaaa tcggcggcca aaaaaccttt accgcccaaa
     1561 cccaattcca aagcggcatc catttatccg ccaaccagac gaactggaac ggcggccaca
     1621 aagcctacat cggcgcggat gccgacaacg cccacatcgt cttcggcgac gacaccctgc
     1681 gcctgcacgg cgcaaacaac cgcatttcct acaacaacca cgacatcttc cacaaagcca
     1741 acaaaccgcg ttttgccgaa gacatcgaag gcaaaccgaa cacactgtcc ggatacggca
     1801 tcggcaattt caaagtcgaa accttccggg gcgatttgaa caccctcaaa acagacggca
     1861 tctattccct gtcgacggcg gtcggcagct ccaacctgcc cgttgaaaac accgcctgcc
     1921 atatccaagt catcgccggc acgaaacacg gctggtgcag gcagttgggt tatcccgcct
     1981 acacgtccga cgtgtacgaa cgccaccaaa cgagcagcgc aaacgacaac tggtccgcat
     2041 ggaaaaaact caattcggac ggcatccccg tcggcgcgat cgtatccttt cccaaagccg
     2101 tccgaaaccc cgcaggctat ctcaaagcca acggcacgac ctttgcacaa aacaccttcc
     2161 ccgacctcta ccgcgccttg ggcaacagca accgcctgcc cgatttaagc cgtaccgaca
     2221 tcggcatgac cgcgtggttt ccgtccgacc aaatcccgac cggctggctg gcgtttgacg
     2281 acatccgcac gcgcgtaacc gaaaccgctt atcccgagct gtaccgtctg ctgaccggaa
     2341 aatacggcag catccaaaac gtcccgcagg cggaagaccg ctttatccgc aacgcaggca
     2401 acagcttggc agtcggaacg aagcaggaag acgaaatcaa acggcacgtc cacaaagtat
     2461 tttcacactg gacaaaccac acagacacgg cagccgtcgg ttacgaagac cacaacgaaa
     2521 ggcagagaag cgcgctcgta tcgacttgga cggacggcga tttaaacgac aacggctttt
     2581 taaccccgcg cccggacagc aaaatggcga caggcggcga cgaaaaccgc cccaaagccc
     2641 tggttttaaa actgtgcatc aaagccgccg acaccttggg cgaagccgtg ttttggataa
     2701 agtcccacgg cgaaaccatc aacgccggcg cgctggacgc gggcacgctg gcgcaaaacc
     2761 tccaagacaa agcagaccgc gcccacaccc acaccgccgc ccaaatccaa gggctggacg
     2821 aaaaaatcag caccgccgtt gccgcgcaat tcacacgcca aaccatcggc ggcgtggata
     2881 ttgtcagatt ccccgacggc acaatgatac agaccggcag ctacaggttc acacgaagcg
     2941 gcggccccat cgaaaacgaa gtcgtcttcc ccgtcgcctt tgccgacggc aacgtcaaat
     3001 gcttcgtatc cgaacgccat tcgggacgcg ttaccggcga tcgaaggcaa cacaactggc
     3061 tgtttatccg cgcaaaaaac cacgccgccg ccattattac caactggtac gaaggcagtt
     3121 gcgactggat ggccatcggc aaagccgcct cgggaaacgc cgccagctcc ccgataggcc
     3181 ccgaaatacc tgaaaccaac gaagaaccgc aaagagagag tggaagaaca tcaaccggac
     3241 cccgaaaccg ccgccgccga gacggcttgc tcgaggcact gcaagactag cgggctgtag
     3301 agatggctgt agagacgggc tgtagagatg gctgtagaga tggctgtaga gacgggctgt
     3361 agagatggct gtagagacgg gctgtagaga tggctgtaga gatggctgta gagacgggct
     3421 gtagagacgg gctgtagaga tggctgtaga gacgggctgt agagatgggc tgtagagatg
     3481 ggctgtagag atgggcttca gcccgccgat ccaagcaatc cgaccgaaac cggccgcgcc
     3541 gccaatcccc cgaaacctat gccccgccaa tcctgccact cttcgtcatt cccgccgctt
     3601 tcgtcattcc cgcgaaagcg ggaatccagc ccctaaacgc ggcaggaatc tatcggaaaa
     3661 aacagaaacc cccgccaccg tcattcccgc gcaggcggga atccagaccc caaacgcggc
     3721 aggaatctat cggaaacggc tgaaaccgaa cggactggat tcccgcctgc gcgggaatga
     3781 cggcggcagg ggtttcggga ttcccgcctt cgcgggaatg acggaaagtg gcgggaataa
     3841 cgaaaggcgg gaatgaccgc gcaaaaagcc gctgccccct tcggacggca ccggcaacaa
     3901 aaaaccgcac ggccgaaacc gcgcgggaaa ggatagtcgg gcgcgcccga taagcagcgg
     3961 ccgcagggtt aagcccttgc acctgtttgc atcgaagaca aaggcggttc gggaaataag
     4021 ggcgcaatat caaaagtttt gcgttgcctg atttgccaca aatcataacc ggcctgcata
     4081 ttcagccaaa aatcaggcga agtattgagc agttggctta aacgaaccgc catctcgggc
     4141 gtaatcgaag attgggcgtt gacaatgcgc gataaggcaa cccggctgac acccaggcgt
     4201 ttcgctatat cggtaatgct tgcaccgtta atatactctt ttaaaacaag gcccgggtgg
     4261 gcaggattgt gcattttcat attattccta gtgataatcc tgataatcga caacttcggc
     4321 attgccgtcg ttgaaacgaa aagtcaaacg ccaatttccg ttgactttaa ccgcccaatg
     4381 tcccgacaaa ctgcccttga ggggatgaag attccaactc ggcacagcca tatccgaagg
     4441 cgtttgcgcg gcattcagcg cggttagaag cagattcagt ttgacggaat gcccggcttg
     4501 gatgcccgat agcgagcctg ttttaaaaaa gcgttcaagc cctttgtgtt tgaaagaaac
     4561 aatcatcatc ccaccccgtc tgtatcgcca aaggatacaa aacttttatt taattgtaaa
     4621 gcgacgatat acaaaaaagg aaaccccgaa atgaccatct atttcaaaaa cggcttttac
     4681 gacgacacat tgggcagcat ccccgaaggc gcggttgccg tccgcgccga agaatacgcc
     4741 gcccttttgg caggacagac gcagggcggg cagattgccg cagattccga cggccgcccc
     4801 gttttaaccc cgccgcgccc gtccgaatac cacgaatggg acggcaagaa atgggaaatc
     4861 ggcgaagccg ctgccgccgc ccgtttcgcc gaacaaaaaa ccgccacggc attccgcctc
     4921 gcggcaaagg cggacgaact caaaaacagc ctcttggcgg gctatcccca agtggaaatc
     4981 gacagctttt acaggcagga aaaagaagcc ctcgcgcggc aggcggacaa caacgccccg
     5041 accccgatgc tggcgcaaat cgccgccgca aggggcgtgg aattggacgt tttgattgaa
     5101 aaagttgtcg aaaaatccgc ccgcctggcc gttgccgccg gcgcgattat cggaaagcgg
     5161 cagcagctcg aagacaaatt gaacaccatc gaaaccgcgc caggattgga cgcgctggaa
     5221 aaggaaatcg aagaatggac gctaaacatc ggctgaaaaa atacgtttac cacctgttgg
     5281 tagccatcga ccaactgttc aacgccctaa ccggcggcgc ggcggacgaa accctctcaa
     5341 gccgcaccta tcgcggcgcg cggctcgccc aaaagcccaa aacccgctgg aaggttttat
     5401 acaccctgat caacggcgtg tttttcgacc gccaacactg tcggcaggcg tatatcagcg
     5461 aactgaaagg caggcagcac gacgcgcggt tcaaccaaag ccgcgccgcc ggggaaaagg
     5521 ggacgcgatg aactacttcg gcatggtaga gtttctgcgc ctgatggcaa tggtgcggcc
     5581 gccatttgtc ttcttctccg gcacccgcag cgaactgcct acttatcttg acctcgtggc
     5641 agaactgcgc ctgaccggat gggagcgttt tgcaggcagc caaaccttga ctgtgagcag
     5701 cacatcaaca gcaattcaag ctacgacgac cacctgattt ataagttctg accgcaagta
     5761 gcgtactact tttaaaggca taagataatc cccgtttaac agaccattaa acggggataa
     5821 atttgtgcaa aagctaatac aatttcctac gcttcggcgg tgcaaaagct gccgccaatt
     5881 cgtgcaaaag ctgccgccgc cttacattcc agtgcaatgc cgtctgaaac ttcgctaatc
     5941 tcgggttgcc gcgcgctgtg ttgttcttcg gtactcagca aaaagccgta cagacgctcc
     6001 aatcgggcgc ggtaggcggt gaatttgttg tagaacatcc ggaaaaaaga tagcgcgttt
     6061 tgcagtcgcg caaaggcttg gacggtctgc tggatgtcgc cgattttgat ttgtccggca
     6121 aacaggcgcg gcgcttgcaa aataatgggg aagagtttga tgccgttggt gaacatatcg
     6181 ttaaagccgc ttaagcagac gctttgccgc gcgatgcgcc aacggttgcg gataatggct
     6241 ttaaagcggt cggaaagccg gccgtgttcg tgttgttcgc cgttgtaaaa cgctacgctt
     6301 tcggcgtggt cgcgtacgcg gatgagggaa taacggtagt cgccgttgag tttttcgttt
     6361 tcatagttgt aacgaatcaa ggggttgcct atccacatcg cgataaaggt cgccaaaatc
     6421 acaaaaataa agacgagcca aacaatgccg cgcggaatgt cgaagccgaa cacggtcagg
     6481 atgccagcca agccccacaa aacaacggca aattccaaag aggtaacgac cgaattgacc
     6541 atgccgcgta caaattcgat ggtcgaagcg atgaaatcct gcgcgtcctg ttggatacgt
     6601 tggtcgatgt tgtccggcgc gtggcggcgc atttgcaggc ggtagtagtt tttgtcggca
     6661 agccagcgcg tcgtcagcac ttcattgagc tgctccgacc atttaatcgc caagccttga
     6721 tcgaggaagt cgttgacgac attgttaaac gcccgtatca gcaccacgcc cgcgttcatt
     6781 gcggcaaaca tccaaaatgc cgaagcattt caaatcctgc atcgagtcgt aaagcccttt
     6841 ggacataaag gtactcaaca cattcagccg catttcggtt aacaccagcg taatcatcgc
     6901 cgtaatcagc agcaagactt tgaccgcgct tttcggtgtc agacaaagcc aaagcggtgt
     6961 ggaataaagc tcggtttgcc atttctgcat gggaaatttc ttacggtatc aatgccgtct
     7021 gaaaaagacg ggtacagttg attttttgat gaagtttggg gaagttttgc cggtcagggt
     7081 acattgcgtg tt
//
LOCUS       DAWEWP010000103         7088 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWEWP010000103 DAWEWP010000000
VERSION     DAWEWP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.103"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(66..425)
                     /gene="rnpB"
                     /locus_tag="WGN53_001487"
     ncRNA           complement(66..425)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WGN53_001487"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(504..1607)
                     /gene="prfB"
                     /locus_tag="WGN53_001488"
     CDS             complement(504..1607)
                     /gene="prfB"
                     /locus_tag="WGN53_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ0140469.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1710..2603)
                     /locus_tag="WGN53_001489"
     CDS             complement(1710..2603)
                     /locus_tag="WGN53_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ0140470.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2945..3394
                     /locus_tag="WGN53_001490"
     CDS             2945..3394
                     /locus_tag="WGN53_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0140471.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3468..3776
                     /locus_tag="WGN53_001491"
     CDS             3468..3776
                     /locus_tag="WGN53_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140472.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3892..4734
                     /locus_tag="WGN53_001492"
     CDS             3892..4734
                     /locus_tag="WGN53_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ0140473.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4724..5470
                     /locus_tag="WGN53_001493"
     CDS             4724..5470
                     /locus_tag="WGN53_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ0140474.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5492..6079
                     /locus_tag="WGN53_001494"
     CDS             5492..6079
                     /locus_tag="WGN53_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ0140475.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6085..6264
                     /locus_tag="WGN53_001495"
     CDS             6085..6264
                     /locus_tag="WGN53_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ0140476.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6496..6936
                     /locus_tag="WGN53_001496"
     CDS             6496..6936
                     /locus_tag="WGN53_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140477.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1808 a         1884 c         1615 g         1781 t
ORIGIN      
        1 caacctaaat aaaaatgccg tctgaaccat atttcaggtt tcagacggca tttgcgtgtc
       61 ggatgcacac cggacaggcg gtaagccggg ttctgtctcg gacagtcatt cctctaggca
      121 taccgttacc agtatgctca agcaacctac ccgaacgctc ggcgggcagc gtcattgcgt
      181 tctgtttggt cttgctccga atggggtttg gcctgccgca tattgttacc aaatgcgcgg
      241 tgcgccctta ccgcaccttt tcacccttgc ctgtgctgcc aaagcagcca tcggcggttt
      301 tgctttctgt tccactttcc gtcgcgttac cgcgcccggc cgttaaccgg cattctaccc
      361 tgcggagccc ggactttcct ccccgtatgc cttacgcgat acgcggcgac tgtctgctcg
      421 tcccgtgtgc ggcgcggatt ataacacgaa acgcaaaaat gccgtctgaa acggtacagg
      481 tttcagacgg catacagcct aaactacacg ccctgtttca ggctggcttc gataaagccg
      541 tccaaatcgc catccaatac ggctttggtg ttgccgactt cgtagcctgt acgcaagtct
      601 ttgatacgtg aggaatccaa aacatacgaa cggatttggc taccccaacc tacatcggat
      661 ttaccttctt ccaacgcctg tttctcttca ttgcgtttgc gcatttccaa ttcatacagt
      721 ttggacttca acatttccat cgcggcggct ttgttggcgt gttgcgaacg gtcgttttga
      781 cattgcacca caatccccgt cggctcgtgg gtaatacgca cggcggagtc ggttttgttg
      841 atgtgctgac cgcccgcgcc cgatgcgcga taggtgtcga tacgcaaatc ggcggggttg
      901 atttcgattt cgatggaatc atcgatttca gggtaaacga acacggaggc aaacgaagta
      961 tggcgtttgt tgttcgagtc aaacggtgaa tagcgcacca agcggtgaac gccagtttcg
     1021 gtacgcagca aaccataggc gtattcgcct tccactcgga tagtcgcgcg gttgatgccc
     1081 gcgatttcgc cgtcgtcttc ttcgaggatt tcgattttga agcctttgcg ctcggcgtag
     1141 cggctgtaca tgcggaacag catacccgcc cagtcttccg cttccgtacc gcccgcgcct
     1201 gcggtgatgt cgataaagca gttgttcggg tcggcgggct ggttgaacat ccgtttgaac
     1261 tccaaatccg ccatctgttt ttccagcccc gccacatctt cctgcacggc ggcaaaacct
     1321 tcttcgtcgt tttcttcgac ggtcatttca atcagcatgc ggttgtcttc gatgcccgaa
     1381 gcgatgttgt cgagcgtcaa cacgatgcct tcgaggattt tgcgctcttt gccgatttct
     1441 tgggcgcgtt tcgggtcgtt ccaaagttcg gggtcttcgg aaagaccgat aacttcttcc
     1501 aatcggtctt tcttaccctg ataatccata taaactcgga tgtcttcgct gcgcttttcc
     1561 aaatcattca gggtattgtt gagctggttg attacttcgg cttccatgat tcttttgttc
     1621 tttcaaaatt ttaggggcgt attgtacggg attcgggtat ttttttctat ggataaagcc
     1681 ttctagaaac acgttcggac ggcatagcgt caataacggt atgccgccag tttgcgtttg
     1741 atttcaggca atgcggcacg tgctgcctcc tcacccaact ggatggcacg ttttttctga
     1801 tcgaatccgc cgactgcacc caaatccaaa acctgcggtt tgataaccac atccgcctgc
     1861 cccaactcat tttgcaacgc ggaaacgctc attacgttca gcgtctgatc gagataagag
     1921 aagaagcctt ggctgatgtt tttgcccgga cgggcggaaa tatcgacggc aatcacgaaa
     1981 ttcgccccct gccgccgggc ggcactgacg ggcacgggct gcgacagacc gccgtcaaca
     2041 tatgtatgcc tgccgatgat aacgggttgg aacacattgg gaatggcggc ggaagcgcgc
     2101 acagcctgcc cggcattccc ttgattgaaa gcgacggcct tgccggtttc aaaatcagta
     2161 gcaacagcgg caaatttgat gggaaactgc tgaatctgcc tgccgccgac ttttcggttg
     2221 atgtaatttt gcagcttttc gcctttgata aaaccactgg tggacaaggt taaatcgact
     2281 aaatcggttt tgcctaaaat ttcggcttcc aattcgaggc ggtcgggcga catacccgat
     2341 gcaaaaaggc tgccgacaat cgaacctgcc gatgtgccgg taaccacctt cacaggaata
     2401 ccgttttctt tcaaaacctt aataatacct acatgggcaa atcctttaga tgcgccgcca
     2461 ccgagtgcca aaccgaccac tgcggcgggt ttggcggttt gcaccggctt gcgggcggca
     2521 ttatttcccg ccgtgccgca ggcggcaagc aacgcggcgg cggcgattgc caaaagcggt
     2581 ctgatttttg aaaacgttac catattttcc attcctttat atatcgcacc ccgtcaaaaa
     2641 gagggattgc ttttcttaac aacccccttt gacagccaag caaatggagg ctttgttaag
     2701 tcatcatcaa aattaatatt tctttttttc ctttacggaa attatatttg aaggcatact
     2761 atccaaggtg ggaattatct cacaacaccg ccgttatcca aatatcccgc ctttttccct
     2821 tcctttccat caaaatactt tctttttata ttcattaact tgttaaatca ttggctgccg
     2881 ggtgtcagtt tttccgacaa aatccgtcta atggggtatc aacagaacca aaacaggaac
     2941 acttatgaaa atcggaacaa cttggcagac ggcatccgct atgctggttt tgcgtctgtt
     3001 tgccgcatat gaatttttgg aatcgggttt gcaaaaatgg aacggggaga attggttttc
     3061 tgaaatcaac gatcaattcc cattcccgtt caacttgctg ccggacgcgt taaactggaa
     3121 tctcgctatg tatgcggagc ttttactgcc cgtactgttg cttttgggtt tggcaacgcg
     3181 tctgtcggca ttagggctga tggtcgttac cgccgtcgct tgggctgcgg ttcacgccgg
     3241 ttcgggttac aatgtctgcg acaacggtta taaaatggct ttaatttata tcgtggtatt
     3301 aatcccgctg cttttccagg gtgcgggcgg atggtcgctg gatacgctgc tgaaaaaacg
     3361 gttttgcccc caatgccgtc tgaagcaaga ttgattcagt cgtggaatct gactttaaac
     3421 attccaacct tatctcgtta acttgatatt ctgaaaagga aatagaaatg aacaaaaaca
     3481 ttgctgccgc actcgccggt gctttatccc tgtctttggc cgccggtgca gttgctgcca
     3541 acaaaccggc aagcaacgca acaggcgttc ataaatccgc ccatggctct tgcggcgcgt
     3601 ccaaatctgc cgaaggttcg tgcggcgcgg ctggttctaa agcaggcgaa ggcaaatgcg
     3661 gcgagggcaa atgcggtgcg accgtaaaaa aaacccacaa acacaccaaa gcatctaaag
     3721 ccaaggccaa atctgccgaa ggcaaatgcg gcgaaggcaa atgcggttct aaataatccc
     3781 accccttcaa accaagccgc gtttttcagt aaaatgcggc ttttttaacg gcaaacaaag
     3841 attttttaac aagcacatca ttcttttgtg ccatccgaac cgggtaaaaa tatgattcaa
     3901 cacgcaggct tgggctaccg ccgcgacttg gcggaagact ttctctcgct ttccgaaaac
     3961 agcccgatat gctttatcga agccgcaccg gaaaactggc tgaaaatggg cggctgggcg
     4021 cgcaaacagt ttgaccgtgt ggcggaacgg ctgccgctgg cgttgcacgg attgtctatg
     4081 tcgctgggcg ggcaagcacc gctggatact gatttgatag acggcatcaa agaaatgatg
     4141 cgccgttacg attgcacgtt tttctccgac catttgagct actgccacga cggcggtcat
     4201 ctttacgatt tgttgccgct gccctttacc gaggaaatgg tgcatcatac ggcgcggcgt
     4261 atccgcgaag tgcaagaccg tttgggctgc cgcatcgccg tggaaaacac gtcctactat
     4321 ctgcattccc cgcttgccga gatgaacgag gtcgagttcc tcaacgccgt cgcacgtgag
     4381 gccgattgcg gcattcatct ggatgtgaac aatatctacg tcaacgccgt caatcacggc
     4441 ctgttgtcgc cggaggcttt tttgaaaaac gttgacgcag atcgcgtgtg ctatatccat
     4501 attgccggac atgacgtgga aacgccggaa ttgttgattg atacacatgg cgcggcagtt
     4561 ttgccgactg tttgggactt gctcgaactt gcctatgcca agctgccgac gattccgccc
     4621 accctgttgg aacgcgattt taatttcccg ccttttgccg aactcgaagc cgaagtcgcc
     4681 aaaatcgccg attatcaaac gcgtgccgga aaggaatacc gccgtgcagc ctgaaacctc
     4741 cgcccaatac cagcaccgtt tcgcccaagc catacgcggg ggcgaagccg cagacggtct
     4801 gccgcaagac cgactgaacg tctatatccg cctgatacgc aacaatatcc acagctttat
     4861 cgaccgctgc tataccgaaa cgcggcaata ctttgacagc gaagaatgga gccgtctgaa
     4921 agaaggtttc gtccgcgacg cgcacgccca aacgccctat tttcaagaaa tccccggcga
     4981 gttcctacaa tattgccaaa gcctgccgct tttagacggc attttggcac tgatggattt
     5041 tgaatatacc caattgctgg cagaagttgc tcaaattccg gatattcccg acattcatta
     5101 ttcaaatgac agcaaataca caccttcccc tgcggccttt atccggcaat atcgatatga
     5161 tgttaccgat gatttgcatg aagcggaaac agccttgtta atatggcgaa acgccgaaga
     5221 tgatgtgatg taccaaacat tggacggctt cgatatgatg ctgctagaaa taatgggttc
     5281 ctccgcgctt tcgtttgaca ccctcgccca aacccttgtc gaatttatgc ctgaggacga
     5341 taattggaaa aatattttgc ttgggaaatg gtcaggctgg actgaacaaa ggattatcat
     5401 cccctccttg tccgccatat ccgaaaatat ggaaggcaat tccccgggcc aaaaccatct
     5461 atccgcataa aattaccttg ttcccgatac tatgccgcta cccgacctga ccgatgccga
     5521 attaatagag tcgcgtaaac tgcttctgca ttttgcgcgg cttcagttgc ccgaccaccc
     5581 tgatttggct gaagatttag tgcaggaaac attgctgtcc gcatacagcg caggcgacag
     5641 ttttcaaggc agggcacttg tcaacagctg gctttttgcc atattgaaaa acaaaattat
     5701 tgacgcatta cgtcaaatcg gaaggcagag gaaagtcttt accacactgg atgacgagct
     5761 actggatgaa gcatttgaaa gccatttttc ccaaaacggg cattggacgc cggaagggca
     5821 gccgcaacat tggaacactc cggaaaaatc attaaacaac aacgaattcc aaaaaattct
     5881 gcaaagctgc ctatacaacc tgcctgaaaa caccgcacgg gtatttaccc tgaaggaaat
     5941 actcggtttt tcatccgacg aaatacaaca aatgtgcggt atcagcacgt ccaactacca
     6001 caccattatg caccgcgccc gagaatcatt gcgccaatgc ctgcaaatca aatggttcaa
     6061 ccaagaaaac ccgaagtaaa cgttatgaaa aaatgccgcg atatcgccct gcttctttcc
     6121 aaacatcagg accgggaaac caccccgggc gagaagattt ccatatacac acacctgctg
     6181 ttctgtccgt attgccgtga atataaaaga caacttcaaa ccatcaaaag atcactggca
     6241 aaaacaacca gaacttcaaa ataaatgccg tctgaaaagg cttcagacgg cataagctga
     6301 cggaaacaaa tcaaaccgat ttactgttat cggcagttca tccataatac acgcttcaaa
     6361 agcagcatat tcctccatac ggaatatata aatacgcaaa acacgaaggc tgcatcaatt
     6421 tgctttattt gccttatttc acagacggcg ctacccctcc cgcccaaccc gttctttctg
     6481 aatgagcaga tttcaatgat taaggaaacc ctaatgcgcc caatcttcct atctttcgtt
     6541 ttactcccta ttttgataac cgcctgcagc acaccggaca agtctgcccg atgggaaaat
     6601 atcggcacaa tctcaaacgg caatattcat acatatatca ataaagacag cgtgagaaaa
     6661 aacggaaatc tgatgatttt ccaagataaa aaagttgtta ccaatctaaa acaagaacgt
     6721 tttgccaaca cccccgcata caagactgcc attgccgagt gggaaatcca ctgcaacaac
     6781 aaaacatacc gcttaagttc gctacagttg tttgatacaa aaaacacgga aatttccaca
     6841 caaaactaca cagcctcttc cctccgcccg atgagcatcc tgtccgggac attaaccgaa
     6901 aaacaatatg aaaccgtatg cggaaaaaaa ctctgattgc aacttataca caaacttacc
     6961 cacaaacctt atcataaaaa tgccgtatga aatactgaaa tatcagcatt tcagacggca
     7021 ttttgccatt ccctgaaaat tatccacaaa gttatccaca ttatttttta aaaccggctt
     7081 ccatccga
//
LOCUS       DAWEWP010000104         7007 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWEWP010000104 DAWEWP010000000
VERSION     DAWEWP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.104"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(75..1496)
                     /locus_tag="WGN53_001497"
     CDS             complement(75..1496)
                     /locus_tag="WGN53_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ0140478.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1854..2213)
                     /locus_tag="WGN53_001498"
     CDS             complement(1854..2213)
                     /locus_tag="WGN53_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ0140479.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2210..2815)
                     /locus_tag="WGN53_001499"
     CDS             complement(2210..2815)
                     /locus_tag="WGN53_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ0140480.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2862..3344)
                     /locus_tag="WGN53_001500"
     CDS             complement(2862..3344)
                     /locus_tag="WGN53_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0140481.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3548..3645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3856..4956
                     /gene="gcvT"
                     /locus_tag="WGN53_001501"
     CDS             3856..4956
                     /gene="gcvT"
                     /locus_tag="WGN53_001501"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ0140482.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRNGQKQFD"
     gene            5119..5505
                     /gene="gcvH"
                     /locus_tag="WGN53_001502"
     CDS             5119..5505
                     /gene="gcvH"
                     /locus_tag="WGN53_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ0140483.1"
                     /translation="MSSNIPAELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDVLLTAEQYAGEVD"
     gene            5657..6904
                     /locus_tag="WGN53_001503"
     CDS             5657..6904
                     /locus_tag="WGN53_001503"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ0140484.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSIQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1783 a         1677 c         1974 g         1573 t
ORIGIN      
        1 tgtcggaagg ggggatattg atttgattat gccggaattt aaaatgccgt ctgaatgttc
       61 agacggcata gcgtttacag cagtttgaaa acgaaaaaga taagggtatg tacgatgaag
      121 acgggtgtca ggaaggcgac cgaccacatc atatagccga agaaagtcgg catcggtacg
      181 ccgcgctgtt cggcaatggc cttgaccatg aagttcggtg cgttgccgat gtaggtcagt
      241 gcgcccatga ataccgaacc catagaaacc gccagcagcg aatgaaacag ggtacccgtc
      301 atcaaggctt gggcatcgcc gcccgccata ttgaaaaaaa cgagataagt gggcgcgtta
      361 tccaagaatg ccgacaatat gccgctcatc caaaaataca tcgtattaat cggatgacct
      421 gccgtatcgt gaaccagcga taccaccccg cccagcgcgc ctgcctcgcc tgctttcaga
      481 atgctcagga cgggaaagat ggtgatgaag atgccgagga agagtttgcc cacttcggcg
      541 atgggttcaa agttgaattc gttgcctgcg cggacttgtt tgggcgtgat tgccatagat
      601 acggcggtca atgcaatcag gatgacatcg cggacgaggt tttgcagggc gtaacggctg
      661 ccgaggattt caaatcccgg gtgttcgggt ttccaaaggc cggacattag aaccgcgccg
      721 accacgcccg aaagcaggag gaagttccat ttgccgaaga tggcgatttt ttcgggtttt
      781 tcctgttgtg ccggcgtatc ttgtgcaatg ctttcctgtt tgaagaaacg gttgtcgatg
      841 aaatagaagg cggtcaacag gacagcggtg ctgatcagga cgggggcgaa catatgtttg
      901 accgtccaca tgaaatctac gcctttgagg aagccgagga agagtggggg gtcgcccaaa
      961 ggggtcagac cgccgccgat gtttgcaacc aggaaaatga agaagatgac gatgtgcacg
     1021 cggcgggtac ggttttggtt ggctttcagc agcggacgaa tcatcagcat tgctgcgccg
     1081 gtcgttccca tgatagaggc aagtgccgta ccgacggcaa gcagggcggt gttgagcttg
     1141 ggtgtgccgt tcaagtcgcc ccaaaccaaa atgccgcctg aaatggtgta cagggcaagc
     1201 agcagcagga tgaaggggat gtattcttca acgagtgcgt gtgcgacggt atggataccg
     1261 gcggacgcgc caaaaaccaa actgaacggg atgaggaaga gcaatgtcca aaaggcggtg
     1321 attttgccgt aatggtgatg ccaggtatgc gaaaaaaaca agggacccaa tgcgatagac
     1381 agcaaaatca gggcaaaggg caggctccac agcaggttta ggtttgcgcc gtccaaatct
     1441 gcggcgtaaa ccgatgcggg gaaaagcatt agtgaaaaca ggggtaggtg gcgcatcgtg
     1501 tttcctcgat tcaagcactg ccttgcgcgg cgcgtgggag tgatacaggc accgtgccgc
     1561 ccggacatag gcggactgtg ctgcctgttt gtttttgaaa aaagttttcc gattgattgt
     1621 aaagtaaata atcaggataa accagtttcc caaaccggaa ggcggcggga aggcggattg
     1681 ctgtgcttgg gaatatatgt tctttttgat aaataatttt atttaaaaca aataaatata
     1741 tgtctttaat aaataatcta tcgaaaacga aaaatgaatt tattttaaca tatatttgca
     1801 atgaaacagg tttgcccccc gtttgtttgc ccttatccct ttcagtacgg cattcaagat
     1861 tcgggcctgc gccacatcca tatggcgaca agggaacaaa aaaccgatga aaccgccccg
     1921 acccaccagc gttggggaaa ctgccaaaac attatcaggc aggatgcggt catcatactg
     1981 atggcgaata ttttggcttt gcgcggcact gcgccgtttt gttcccagtt atgaaccatc
     2041 ggaccgaaat agcggtgccg gtgcagccag cggtgaaagc gcggggatgc ctttgcccag
     2101 caggcggcgg agagcagtac gaacggcgtg gtcggcaaca gcggcaaaaa aatgccgatg
     2161 atacccaaca gcagggagat gcagccgcag gcaattaaaa gataacgtat cattttgaaa
     2221 tatttttctt attgtgcgga taagggcagg atgtgatacc gagttttgcc cagccttcat
     2281 gtcccatttt ttccagcagg gcgatattgc gttcgaatat tgccgaagcg tcgggaaagg
     2341 cttgtgcggc tttggcaatg ctgtcttcgc ggatgaggtg cagcgtcgga tagggagaac
     2401 ggttggtgta gttgccgatg tcgtcgggtt cggtattctg aaagcggaat tggggatgga
     2461 acggtgcgat ttggacgatg ccttccaagc cgttttcgat aacggcggca tcggcaatat
     2521 cgagcatatc gttgaatacg tcgaaatcgg ggaatagggt cgggtgaacc agcagggtgg
     2581 tttccagttc ggtggcgggt gtattgccca gtcgctgcag ttcttcgtcc aagtcttcca
     2641 aaaaactgtc aaggtgtttg gcttcgctga ttgcgatgcg gacaaggttt ttaacgtggg
     2701 gggctttggc aaagggacac aggttcagac cgatgacggc tttttccaac cattgtccgg
     2761 tgtgttcggc aacagcatct ttattttcgg aagtattgat attcattatt gtcatgtaaa
     2821 tgtgtttgca gattgcacgt gcgggaaaat cgggaagggc actattcctt cagcaggtgg
     2881 ttgagcggca gggaggtggt gtgtttgatt tcttttaaaa caaagctcga ttgcgcatct
     2941 tgtacgccgt ggtgggacag gagcgtatcc aaaacaaaat gggaaaacgc gttcatatcg
     3001 gtaaaaaacg cctgaagcag gtagtcggtt tcccctgtca gggcgaagca gctcaagact
     3061 tcaggccatt ttcgaaccga tgcggcaaag tcttcccgcg cgtcttttgc tttgcggatg
     3121 gaaacgcgga taaatgcctg aagccccagg ttgacggatt ccggagacag cagcgcggca
     3181 tattggcgga cgatgccggc atcttccaac tgcttcagac ggcgcaggca cggagaaggc
     3241 gaaagtgcga cacgttcgga cagttcgaca ttggtcagcc tgccgttttc ctggagaacc
     3301 tgtaagattt taatatcggt tttgtctaaa gtgagttggg gcatatttgc gttccgtttt
     3361 aaggaattcg gattgtctgt ccgtatgttt gcggcaatcc gcacagatgg agaccatatt
     3421 aacatataaa aagttatacc gtcatccggg acaaattttg ttttcggaaa atcatgtgaa
     3481 aacagaggcg gtcggtttgc atctctttta agacggcttg cccaaaccgc cgattcaaga
     3541 cataatcggg aaatgtgcag gagagtgtta cacccaacta caatgtaacc accgaaggcg
     3601 cagacaccct taaatcgctc aggtatcagg gactgcacat tgaaacaaac aatctggaga
     3661 gcggcgttgg aataacgtcc accgaagggg agaaggccgt ctgaaccacc attcagacaa
     3721 ccgcgcaaag cagtgagcag actggtttgc catcatgcgg atacggccga aaatctcagg
     3781 ttcaaggaca gatagggtca tccgcgcaca ggtgcgcggg tggcatctga acaaaaaatc
     3841 cggagaaact tgagaatgat tactctgaaa accaccccat ttcatcaagc ccatcaagat
     3901 gcaggcgcga agccggtaga ttttgccggc tgggagctgc ccatccatta tggttcgcaa
     3961 atcgccgaac acgaagccgt gcgcaccgac gccggtatgt ttgacgtatc ccatatgctt
     4021 gttaccgacg tagcaggcgc aaatgccaaa gcctttttcc gcaaattgat tgccaacgat
     4081 gtcgccaagc tcgcttttgt cggcaaagcc ctttattccg ctttgctcaa cgacaacggc
     4141 ggcgtgattg acgacttgat cgtttaccgc accaatgaag ccgaaaccca ataccgcatc
     4201 gtgtccaacg gcgcgactcg cgaaaaagat acggcacaat tccacaaagt cggacaagag
     4261 ttcggtgttt ccttcaatcc gcgctacgac ctcggcatgc tcgccgtaca aggccctaaa
     4321 gccattgaaa aactcctgac cgtcaaaccc gaatgggcag atgtcgtcca taacctcaaa
     4381 ccgttccaag gcgcggattt gggcaacgac tggtttgtcg cccgcaccgg ctacaccggc
     4441 gaagacggcg tcgaagtcat cctgcccggc accgaagccg tcgcattctt caaagccctg
     4501 caagcagcag gcgtacaacc ttgcggtctc ggcgcgcgcg acaccctgcg catggaagcc
     4561 ggcatgaacc tctacggcaa cgatatggac gacgatacca gcccgctcga agcaggcatg
     4621 ggttggaccg ttgatttgaa agacgaaagc cgcgattttg tcggcaaagc tgccttgttg
     4681 gcattgaaag aaaaaggcgt tgccgtcaaa caggtcggtt tgctgctcgg caaaggcggc
     4741 atcctgcgcg cgcatatgga agtgttgacc gacaaaggca aaggcgaaac caccagcggc
     4801 gtattctctc caagtctgaa acaatccatc gccatcgccc gcgtaccgaa agattttgac
     4861 ggcgataccg ccaaagtgct gattcgcggc aaggaagcgg acgtgcgcgt actgaagctg
     4921 ccgttcgtcc gcaacggtca aaaacagttt gattaatatc ggttcagacg gcattttcat
     4981 ttcatatgcc gtctgaaagc aggttttaat tgtcgtccga tacggacgtt tgtagaaagc
     5041 attgaacaag gcatctgtgg atattgattc atgcagatgc cgtctgaaaa taacccccta
     5101 tcaatggagt atcaaaccat gagcagcaac atcccggcag aactgaaata cgttgccagc
     5161 cacgaatggc tgcgccttga agaagacggt atcatcaccg tcggtattac ccaccacgcg
     5221 caagagctgt tgggcgacat cgtgtttgtc gagctgcctg aagtcggcgc aaaccttgcc
     5281 gccgaagaac aagccggtgt ggttgagtct gtaaaagccg cgtccgacgt gtacgcaccg
     5341 attgcaggtg aagtcgttgc cgtcaacgaa gatttgccaa gcgctccgga aactgccaac
     5401 agcgatcctt acggtgcagg ctggttcttc aaactcaaac cggcaaacgt tgccgattac
     5461 gacgttctgc tgactgccga acaatacgcg ggcgaagtgg attaaaccgc ccggctgccc
     5521 gatggcaacc gccggacaaa cggaaactgc accttcagac ggcatttttg cggtcggagg
     5581 tgcagttttt tgtccgtgtt ttaaggaagc agttaggcta taataacggt ctatattcat
     5641 ctttaccgat tttttcatgc aacttaccgc tgtcggactc aatcatcaaa ccgcaccttt
     5701 aagcatacgg gaaaagctgg cgtttgccgc ggcctgcctg cccgaagccg tccgcaatct
     5761 tgcccgaagc aatgcggcaa cggaggcggt aatcctttct acctgcaacc gtaccgagct
     5821 ttactgtgta ggtgattcgg aagaaatcat ccgttggctc gcagactatc acagccttcc
     5881 catagaagaa atcagcccct acctttatac tttggggatg caggagactg tgcgccatgc
     5941 tttccgcgtc gcctgcggct tggattcgat ggtgttgggc gagccgcaga ttttaggaca
     6001 gattaaggat gcggtcaggg ttgctcaaga gcaggaaagt atgggtaaga aactcaatgc
     6061 cctgttccaa aaaacctttt ctgttgctaa agaggtccgt accgatactg ccgtcggcga
     6121 aaactcggtt tccatggctt ccgcttccgt caagttggca gagcagattt tccccgacat
     6181 cggcgatttg aatgtcttgt ttatcggtgc gggtgagatg attgagctgg ttgccactta
     6241 ttttgccgcc aaaagtcccc ggctgatgac ggttgccaac cggacgctgg cgcgtgcaca
     6301 ggagttgtgc gacaagctcg gtgtcaacgc cgaaccgtgc ctgctgtccg atctgcctgc
     6361 cattttgcat gagtacgacg tggtggtttc ttcaacggca agccagttgc ccattgtcgg
     6421 caaaggtatg gtggagcgcg cattgaaaca aaggcagagt atgccgttgt ttatgcttga
     6481 cttggccgtg ccgcgagaca ttgaggcgga agtcggagat ttgaacgatg cctatcttta
     6541 tacggtggac gatatggtca atatcgtcca aagcggcaag gaggcaaggc agaaggccgc
     6601 cgccgccgcc gaaacgctgg tgtccgagaa ggttgccgaa tttgtcaggc agcagcaggg
     6661 caggcagagt gtcccgttaa tcagggcatt gagggatgag ggagagaaag cgcgcaaaca
     6721 ggtcttggaa aatgcgatga aacagcttgc caaaggcgca acggcagaag aggttttgga
     6781 aaggctgtcg atccaactga ccaacaagct gctgcattcg ccgacccaaa ccttgaataa
     6841 ggcgggggaa gaagataaag atttggttca cgccgtcgcg cagatttatc atttggacaa
     6901 ataacggtgc gccggaaaag gatgccgtct gaaggggttt cagacggcat tttttgtgca
     6961 ggccggcaac atcgtgaaat cccacattat atcgatgtaa tcacaaa
//
LOCUS       DAWEWP010000105         6981 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWEWP010000105 DAWEWP010000000
VERSION     DAWEWP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.105"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            80..589
                     /locus_tag="WGN53_001504"
     CDS             80..589
                     /locus_tag="WGN53_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ0140485.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMEKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            932..1372
                     /gene="nadR"
                     /locus_tag="WGN53_001505"
     CDS             932..1372
                     /gene="nadR"
                     /locus_tag="WGN53_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ0140486.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            1487..1987
                     /gene="hpaC"
                     /locus_tag="WGN53_001506"
     CDS             1487..1987
                     /gene="hpaC"
                     /locus_tag="WGN53_001506"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ0140487.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTNLSPEERFAY
                     HIWHYGKTGQPEIEGALAHLHGHIVGQHEIGTHFVFYVRLDEIKNCVGKRPALLYFRR
                     QFRSLD"
     gene            2005..2700
                     /locus_tag="WGN53_001507"
     CDS             2005..2700
                     /locus_tag="WGN53_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ0140488.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(2931..3362)
                     /locus_tag="WGN53_001508"
     CDS             complement(2931..3362)
                     /locus_tag="WGN53_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0140489.1"
                     /translation="MSSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPID
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            3537..5213
                     /locus_tag="WGN53_001509"
     CDS             3537..5213
                     /locus_tag="WGN53_001509"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ0140490.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAVLLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGVGGADLARKVVNAIENQPNNFNFSYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSSGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            5327..6418
                     /gene="ychF"
                     /locus_tag="WGN53_001510"
     CDS             5327..6418
                     /gene="ychF"
                     /locus_tag="WGN53_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ0140491.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            6456..6662
                     /locus_tag="WGN53_001511"
                     /pseudo
     CDS             6456..6662
                     /locus_tag="WGN53_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6678..6782
                     /locus_tag="WGN53_001512"
                     /pseudo
     CDS             6678..6782
                     /locus_tag="WGN53_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6793..>6981
                     /locus_tag="WGN53_001513"
     CDS             6793..>6981
                     /locus_tag="WGN53_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0140492.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTMILHREMSGGGG"
BASE COUNT         1630 a         1792 c         1961 g         1598 t
ORIGIN      
        1 agataaccgg aagccaagtt ttgagacttg gctttttgtt tgcctgccgg ttgcgcgggc
       61 gtaccgtttg agagagcgta tgaaaaagaa actgctttcg ggcatcaaat ttgccgttca
      121 gacggcattg gtgtttttat tggtgtcgct gtttttggat tggatacgca agcccgaaga
      181 gccggccggt gcggcagggc ggcctttgac tctgctgtcg gggcagcggc tgactttggg
      241 acagtttagc cgagataagg cggttttggt gtatttttgg gggagctggt gcggcgtgtg
      301 ccgttatcag tcgccgataa tcgatgattt ggcggcggac ggcgttccgg tcgtcggcgt
      361 ggcggtacgt tccggcagtt cggcggaagt ggcggcgtat atggaaaaac gcggcttggg
      421 ctttccgacg gtcaacgatg aggacggggg tttggcaagg tcttggcgga ttgtcgcaac
      481 gcctgccgtc gttttggtca aaaatgggaa aatggttcac tatacgacgg gaatcagcag
      541 ttattggggc ttgcgcgcac gaatttttca ggcggatgtt ttcggttaac ctttgttttt
      601 attaaatttt ccgctatgta gagagtcaaa cggcatatgc cttgcgcggc ggatgcggcg
      661 gcttttgttt tttctggaag aaagttcttt cgacagtaaa tgtaatactt tgttgagatt
      721 gtagggaaaa tagactaaga tataactatt aaaatatttt tagataggat tatcggaatt
      781 aaagtctttt atacccagtc gtccgatgcg gtttatagcg tattgttgct atatgttcgt
      841 tttgttatat aacggttgca tcaaaattta cgcccacagg ctttcccgac ggtttgaaag
      901 tttgattttc gataacttgg agacttgaac aatgcctacc caatcaaaac atgcgtctat
      961 caatatcagt ctgatacagg caagggaagc cctgatgacc caattcaggc ctattctgaa
     1021 tcaggcgaat attaccgatc agcaatggcg gattatccgt cttttggcgg aaaacggcac
     1081 gctggacttt caagatttgg cgaatcaggc gtgtattctg cgccccagcc tgaccggtat
     1141 cctgacccgc cttgaaaaag cgggtttggt tgtccgcctg aaaccttcca acgaccaacg
     1201 acgtgttttt ctgaagctga ctgccgaggg cgagaagctg tatgaggaaa tcggcgaaga
     1261 agtggacgag cgttacgacg ctatcgagga agtgctgggc cgcgagaaaa tgctgctgct
     1321 taaagacctg ttggcagaac ttgccaaaat cgaggatgcg ttgaactcgt aatacgccgt
     1381 aacgcgcgga aatgtccgac cgacggcttt ttgaatcaga actgctgcac atggggggtg
     1441 ccttgtgtgc agcattctta tataggggac agtttaaagg ggaaaaatgg cggatttgca
     1501 gaaaactttt caaacttcgt tccgtgatgc gatggcatct tgcgcggcag gcgttcatgt
     1561 catcacgaca gacggtgcgg cagggcgtta cggcattaca atgacggcgg ttgcgccggt
     1621 taccgacgag ccgccgaccg tgatgctgtg catcaaccgg agtgcgcgaa tcattccgat
     1681 cctgtcggaa aacggcagcc tctgcatcaa tatgctggcg gacgaacatc aggatgttgc
     1741 cgaacatttt gccggattga ccaatctttc acccgaagaa cgttttgcct atcatatttg
     1801 gcattacggt aaaaccggac aacctgaaat agaaggcgcg ttggcgcatc tgcacgggca
     1861 tattgtcggc caacatgaaa tcggcacaca ttttgtgttt tacgtcaggc tcgacgaaat
     1921 taaaaattgc gtgggcaaac gtcccgcgct gctttatttc agacggcagt ttagatcttt
     1981 agactgatat tcggacagat atatatgaaa gcgatgatac tggcggcagg acgcggcgag
     2041 cgtatgcgtc ctttgaccga taccactccg aagccgctgc tcgatgtggc gggtaagcct
     2101 ctaatcggtt ggcacctatg ccgtctgaag caggcggggt ttaccgaaat cgtcatcaac
     2161 cacgcttggc tgggtcggca gatagaagat gctttgggcg acggctcggc ttatggcgtg
     2221 aacatcgcct attcgcccga acccgcaggc ggcttggaaa cggcaggcgg catcgcgcag
     2281 gcattgccgc tgttgggtgg gcagccgttt ttggtggtca acggcgacgt gctgaccgac
     2341 atcgatttta ccgccgcgtt tcaggcggca tcgtccctgc cggaacatat ttccgcccat
     2401 ctgtggctgg tggaaaatcc gccgcacaac cccgacggcg atttttccct gctgcccgac
     2461 ggcagcgtgc ggccggaagt aaatggcggc aacggattga cattcagcgg cgtgggtatt
     2521 taccgtcctg aaatgtttga cggaatcgaa gcgggcagtg tggcgaaact cgcgcccgta
     2581 ttgcgtggcg aaatgcggca aaaccgcgtg agcggtcaga agcatacggg cttgtggctg
     2641 gatgtcggca cggtatgccg tctgaaagag gctcaagccc ttgcaggggc ttggaagtga
     2701 aataaaacgg tggcttgccg agccggctgc cgaacggttc cgaccggatg tccagcatat
     2761 tttcgtggag gcgtgaaaat agggcgttcg gggctttccg tccaacaaat gccatctgaa
     2821 gcgcggcggt ttgccgtcgg cgcggtgtcg gattttattg ggtaactttg ttcagaccgc
     2881 cgcccgtcag attgtcaaaa cccggtttca ggcggtatgg cggattcggt ttaacgtttc
     2941 aacgccagca ccaacacgcc cgccgttacc agccccaagc ctatccattc ctgcgtgttc
     3001 gggcgttcgt ccaagaaaac caccgccatc agcgcgacca agaccaggct gaatttgtcg
     3061 atgggggcga cttgcgaggc gttgcccagt tgcagggctt tgaagtaggc gagccaggat
     3121 gcgccggtag caaggccgga taggataagg aatgtccagt tgcgccccgt aaagccgttc
     3181 acaccctgcc atttgccggt gtaggtcaaa aacaatacca aagcggcgag gatcaccaag
     3241 gtgcggataa aggtggcgaa atcggaatcg atgccctgta aacccatttt ggcgaaaacg
     3301 gcggtcaatg aggcgaagcc tgccgatgcc aatgcccaaa acagccatgc gttgctgctc
     3361 atgttttctc cttggattgt gaacaatatg aacggtattt ttgttgcagc gtcaaaaatt
     3421 tcactgcggg tttggtgcgg ataacgttat aatatgcctg atattatttt caatccacct
     3481 gtttgtcgcc tgatgctttc agacggcatg tccctcctca tttctaaagg aaaatcatga
     3541 gcttcaaaac cgatgccgaa atcgcccaat cctccaccat gcgcccgatt ggcgaaattg
     3601 ccgccaagct gggtctgaat gccgacaaca ttgagcctta cggtcattac aaggcgaaaa
     3661 tcaatcctgc cgaagcgttc aaactgccgc aaaaacaggg caggctgatt ttggttaccg
     3721 ccattaatcc gactccggcg ggcgaaggca aaaccaccgt aaccatcggt ttggcggacg
     3781 cgttgcgcca catcggcaaa gatgccgtga ttgccctgcg cgaaccttct ctggggccgg
     3841 tgttcggcgt gaaaggcggc gcggcaggcg gcggctatgc ccaagttttg ccgatggaag
     3901 acatcaacct gcacttcacc ggagattttc acgccatcgg tgcggcaaat aatctgcttg
     3961 ccgcgatgct cgacaaccat atctatcagg gcaacgagtt ggacatcgac cccaaacgcg
     4021 tgctgtggcg gcgcgtggtc gatatgaacg accgccagtt gcgcaacatc atcgatggca
     4081 tgggtaaacc cgttgacggt gtgatgcgcc ccgacggttt tgatatcacc gttgcctccg
     4141 aagtgatggc ggtattctgc cttgccaaag acatcagcga tttgaaagag cgtttgggca
     4201 acatcctcgt cgcctacgcc aaagacggca gccccgttta cgccaaagat ttgaaggcac
     4261 acggcgcgat ggcggtcctg ctcaaagatg cgattaagcc caatttggtg caaaccatcg
     4321 aaggcacgcc cgccttcgta cacggcggcc cgttcgccaa catcgcccac ggctgcaact
     4381 ccgtaaccgc aacccgtctg gcgaaacacc ttgccgatta cgccgtaacc gaagcaggct
     4441 tcggcgcgga tttgggcgcg gaaaaattct gcgacatcaa atgccgcctt gccggtttga
     4501 aacctgatgc ggcggtcgtc gtggcgactg tccgcgcttt gaaatacaac ggcggcgtgg
     4561 aacgtgccaa cctcggtgaa gaaaacctcg aagccttggc aaaaggtttg cccaacctgc
     4621 tgaaacacat ttccaacctg aaaaacgtat tcggactgcc cgtcgtcgtt gcgctcaacc
     4681 gcttcgtgtc cgactccgat gccgagttgg cgatgattga aaaagcctgt gccgaacacg
     4741 gcgttgaagt ttccttgacc gaagtgtggg gcaaaggcgg cgtgggcggt gcagacttgg
     4801 cgcgcaaagt cgtcaacgcc atcgaaaacc aaccgaacaa cttcaatttt tcctacgatg
     4861 ttgagctgag tattaaagac aaaatccgtg cgattgccca aaaagtgtac ggcgcggaag
     4921 atgttgattt cagcgcggaa gcgtctgccg aaatcgcctc gctggaaaaa ctgggcttgg
     4981 acaaaatgcc gatttgcatg gcgaaaaccc aatattcttt gagcgacaac gccaaactct
     5041 tgggctgccc tgaaggcttc cgcattaccg tgcgcggcat taccgtttct tccggtgctg
     5101 gtttcatcgt tgcgttgtgc ggcaatatga tgaaaatgcc gggtctgccc aaagttcccg
     5161 ccgccgagaa aatcgacgtg gacgcagaag gcgtgattca cggcttgttc tgaacggttt
     5221 ttgaaaccag atgccgtctg aagccgtttc agacggcatt ttttcggaac gcgggcggcg
     5281 gtatgctata atccgccgtt aaatttctct attttcagga aaaaacatga gtttgaaatg
     5341 cggcatcgtc ggtttgccca acgtcggcaa atccaccctt tttaacgcgc tgacccaatc
     5401 gggtatcgaa gcggcaaact atcctttctg taccatcgaa cccaacgtcg gcatcgtcga
     5461 agtccccgat ccgcgtatgg ccgaattggc aaaaatcgtc aatccgcaaa aaatgcagcc
     5521 tgccatcgtc gaatttgtcg atattgccgg tttggttgca ggcgcgagca aaggcgaggg
     5581 cttgggcaac cagttccttg ccaacatccg cgaaaccgat gcgattgtga atgtcgtgcg
     5641 ctgctttgac gacgacaaca tcgtccacgt tgcaggccgc gtcgatccga ttgccgacat
     5701 cgaaaccatc ggcacagagt tggcacttgc cgacctggca agtgtggaaa aagccatcgt
     5761 ccgcgaagaa aaacgcgccc gttcaggcga caaagacgcg caaaagctgg tcgatttgtg
     5821 caaaaaactg ctgccgcatc tggacgaagg caaacccgtg cgttccttcg gtttggacgc
     5881 ggaagaacgc gcgatgctca aaccgctgtt cctgctgacc gccaaaccgg cgatgtatgt
     5941 gggcaacgtc gccgaagacg gttttgaaaa caatccgcac ctcgaacgcc tgaaagaatt
     6001 ggcggcaaaa gaaaacgccc ccgtcgtcgc cgtttgcgcc gcaatggaaa gcgaaatcgc
     6061 cgaattggaa gacgacgaaa aagccgagtt cctcgccgaa atgggcttgg aagaaccggg
     6121 cctgaaccgc ctgatccgtg ccggctacga ccttttgggg ctgcaaacct actttaccgc
     6181 cggcgtgaaa gaagtccgcg cttggacgat acacaaaggc gacaccgccc cgcaagccgc
     6241 cggcgtgatt cacaccgact ttgagcgcgg cttcatccgc gcccaagtca tttcttacga
     6301 cgactttgtc tcgctcggcg gcgaagccaa agccaaagaa gccggcaaaa tgcgtgtgga
     6361 aggcaaggaa tacgtcgtgc aggatgggga tgtgatgcac tttttgttta acgtgtaacc
     6421 caaatgcggc aggttttcag acggctttgc cgaaaatgcc gtctgaagtc ggttttggtg
     6481 gctttcggcg tttccatacc gccggaatgc ggctgcatca aaataaaatc ccgcccgcat
     6541 ttccgatttg ccctccccga ttcctgcaaa acaaaccgcc tgccccgctg ttgcaaaaag
     6601 ccgtccggca ttccgaatat cccgagcccc gatacaaaat gacctttcag acggcatttg
     6661 cgccgccgcg tttcgagtat agtggattaa ctttaaatca ggacaaggcg acgaaaccgc
     6721 agacagtaca gatagtacgg caaggcgagg caacgccgta ctggtttaaa tttaatccac
     6781 tataaaaaca ttatgagcca agccttaccc taccgcccgg acatcgacac attgcgcgcc
     6841 gccgccgtct tgtccgtcat cgtgttccat atcgaaaaaa attggctgcc gggcgggttt
     6901 ctcggtgtcg atatattttt cgtgatttca ggctttttga tgacgatgat tcttcaccgc
     6961 gaaatgtcgg gggggggggg g
//
LOCUS       DAWEWP010000106         6970 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWEWP010000106 DAWEWP010000000
VERSION     DAWEWP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.106"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            337..636
                     /locus_tag="WGN53_001514"
     CDS             337..636
                     /locus_tag="WGN53_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0140493.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPAGMAVSSLPPQPH"
     gene            576..812
                     /locus_tag="WGN53_001515"
     CDS             576..812
                     /locus_tag="WGN53_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0140494.1"
                     /translation="MRLRDAGGYGGFFPAAAAALTQRPYATLDLTAHYKIGKSTRIGL
                     DFENVFNKRYRPMPDIHVYGTPRSLTATVKHIVD"
     gene            complement(1004..2407)
                     /gene="mtrE"
                     /locus_tag="WGN53_001516"
     CDS             complement(1004..2407)
                     /gene="mtrE"
                     /locus_tag="WGN53_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ0140495.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(2462..5665)
                     /gene="mtrD"
                     /locus_tag="WGN53_001517"
     CDS             complement(2462..5665)
                     /gene="mtrD"
                     /locus_tag="WGN53_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ0140496.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(5677..6915)
                     /gene="mtrC"
                     /locus_tag="WGN53_001518"
     CDS             complement(5677..6915)
                     /gene="mtrC"
                     /locus_tag="WGN53_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ0140497.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1616 a         1953 c         1827 g         1574 t
ORIGIN      
        1 atcaaacatc aaattggact gaccaatcag ggcggattct aatgacacgc cgttcccgcc
       61 gtcaacggca tttacctcgc accgcccccg aaacacaaga aaaaactaca ccaaactaca
      121 atttttgttt atacaaatat ttgttttgac aggatttaaa cgaaagctcc gattcaaatc
      181 tgccgaaccg cccaaaaata tatagtggat taacaaaaat caggacaagg cgacgaagcc
      241 gcagacagta cagatagtac ggtaaggcga ggcaacgccg tactggttta aatttaatcc
      301 attctattga cctgaatatt aaagtttcgc aaagtaatgc aacgttgctt taattggttt
      361 gaccactatt gccgacgatt agaaaaatat tttcggagat gttcaattat ggaaacttgg
      421 gttcaaaact acacggcaat cggcggcagc ctgtatctga ctgccgccgc cgcactctta
      481 cccatcgtct ttttctttgc cgcgctgacc gtcctgaagc tgaaaggcta tcaggcgggg
      541 ctttatacgc tgctgattgc gcttgccgtt gccgtattcg gcttcgggat gccggcgggt
      601 atggcggttt cttccctgcc gccgcagccg cactgaccca acgcccctac gccacgctgg
      661 atttgaccgc gcattacaaa atcggcaaat ccacccgcat cggtttggac tttgaaaacg
      721 tgttcaacaa acgctaccgc cctatgcccg acattcacgt ttacggcacg ccgcgcagcc
      781 tgaccgcaac cgtcaaacat atagtggatt aacaaaaatc aggacaaggc gacgaagccg
      841 cagacagtac aaatagtaca acttggtgct tcagcacctt agagaatcgt tctctttgag
      901 ctaaggcgag ccaacgctgt actggttttt gttaatccac tataaagaaa gaaatgccgt
      961 ctgaaacctt atcgtttcag acggcatttt attgcccgac cggttatttg tcggtttggg
     1021 tatcccgttt caatccgccg ccgagtgcct tgtacaaatc ggcaaggttt tcggcgcggg
     1081 tcagttgtgc cgacaaagcc gcaccttccg ccgaatagct gctgcgttcc gcatcgagca
     1141 agtcgagcgc gccggatacg ccgtgtttgt aacgcagacc gaccaaacgc aacgcttctt
     1201 tagaggcgcg gctttgtttg cttaaagcgt cataggcttt atccaactgc tcgcgcgccg
     1261 ccaatgcgtt tgccacgtct tgaaatgcgg attggacggc ggattcatag gcaacgattt
     1321 gtgcctgttg gcgcagcttg gctacatcaa ggttcgcctt gttcgtaccc caggtaaaaa
     1381 tcggcagggt aatagacggc gcgaacgacc aaacgcccgt gccgcttttg aacaacccac
     1441 ccaattcggc agaacccgta ccgacggttc cggtcaggcg gatggatggg aaaaaggcgg
     1501 cgcgtgccgc accgatattg gcgtttgcct gtttgagcgc gtgttcggca gcacggatat
     1561 cgggacggtc gagcaatact tcggaactca aaccggccgg cagtttttca acaaaaaact
     1621 gcttgtccag cggcaaaccg gcaggcaggt cttcgggtat cggttggtta atcaaggttg
     1681 ccaaggcatt gcgcgcctgt tcgcggctgc gcgcggcatg ggcataatcg gctttggcag
     1741 attcgattag ggcttcctgc tgacgtaggg cgacggcgga aatcacgcct gccttgtaac
     1801 gtaattcgga cagcttgtag gtttcctcgc gcgttttcaa aacacgttgc gccaaagaca
     1861 tcgcttcttc ggcgtaacgt tcgttgaaat aggctttggc aacggtggca atcaggctca
     1921 aatgtgccgc atcgcggttg gcggtgctgg cgaaatagcc ttgcagtgcc gcctcgctgc
     1981 tgctgcgtac acgcccgaac aaatcgagtt cgtaagatgc cgcacccagt ccgactttgt
     2041 agctgctgct tacattgccg ccgctcaagc tgccttggcg cgagtcgttc gcattggcgg
     2101 caagcgtggg caggaggttg ttgcgctcaa tcatgtattg tttgcggtag atttcgctgt
     2161 tcaatacggc ggtacgcaaa ctggtattgc gctcgagtgc gatgtcgatc agcttttgca
     2221 ggcgcgggtc ggcaaaatag tcatgccaac ctaaatcgac cgcacggatg ccgctgtcgg
     2281 cggtatcgtt tttgaacgtt tcggcaactt cgactttggg ctgctcgtat tggggaatca
     2341 tggtgcaggc ggataatgcg aaggctgctg caacagaagt caaggtggtt ttcaatgtag
     2401 tatccataaa aaagtcctga tgccgtctga aaacccgtag gcgttcagac gtcatggttg
     2461 cttaatgttg gctgtcgtcc gaaccggtga tgcccgcttc ggcggcgtgt tttactgcca
     2521 tttcgtgttc gtgcgcggtt tctttgaaga atttgcgcac caccacatag aaaagcggaa
     2581 caaggaacac ggacaagagc gtgccgatga gcatccccca gaatacggtc gtgccgatgg
     2641 cgcgctggct ggcagaactt gcaccgccgg caatatacag gggaaccacg cccaaaataa
     2701 aggcgaacga ggtcatgata atcggacgga aacgcaggcg ggcggcttcc aaagcagctt
     2761 cgaccgcgct tttcccttgc gcttgaaggt ctttggcaaa ttcgataatc aaaatcgcgt
     2821 ttttcgcact caaacccatc acggtaacga aaccgacttg aaagtagatg tcgttggcga
     2881 acgagggaac gctgcccaac agtccttcaa acaggttgcg cccggttacg cccgcagccg
     2941 caccgatcaa acccaacgga atcacaagga tgaccgccag cggaatcgac cagctttcat
     3001 aaagcgcggc aagtaccaaa aatacggctg caaccgccaa accgtacaaa atcagggttt
     3061 gcgagccgcc ttttgcctct tcgcgcgact gtccgcccca ttcgaagctg taaccgccgc
     3121 ccaattcgtc aaccattttt tgaaccgccg ccatagcctg cccggtggaa acgccggttg
     3181 caggcgaagc ggacagcttc atcgaaggat aaccgttgaa gcgtacgctc tgttccgtac
     3241 cgttttccca agaaacagta gcaatggtgg aaagcggtac ggcgacgccg gatttgttcg
     3301 gcacggtcag gttcaaaata tcggcaggct gcatacgggc atcctcgtcg gcctgcacca
     3361 tcacgcgttg cagacggcct tggttcggga agtcgctgac ataagacgaa ctcagcgcac
     3421 ttgccaatgc ggtgcggatg tcggcaaacg aaatgccttg cgccgccgcg gcggcacggt
     3481 tgatgtcgat tttcaactgc ggcgagtctt ccaaaccgcc ggcacggacg gtgctggggt
     3541 caaacaaacc gctggcacgc attttctgaa tcaactcgtt gcgcttcgcc agcaatgcgg
     3601 tatggccggt attgttgcgg tcttgcaggt tgatgctcag gcccgaacca ttgcccaact
     3661 ccaaaatcgg aggcgggacg acggcgatgc caaaaccgtc tttaagcgtc cccatcatca
     3721 tacccgtcag cttgccggca atcgcaacgg catcgctgcc gggcgcggta cgctcgttcc
     3781 aatctttcaa tatggcaaaa cccatcgcca tattctgacc gctgcccgaa aagctgaagc
     3841 cggaaacggt aatgatgttt tctatttcag gaatgctttt cgccagttgg gtaacttgcg
     3901 ccaaagtcgc attggtgcgc tcttgggtcg ctcctgcagg cagttgtacg ctgaccatga
     3961 cgaagccttg gtcttcggtc ggcaggaacg aagtcggcag gcgcataaac aggaacacgc
     4021 ccacaaccgc caagccgata tagacaacca tcatgcggaa agtcttacgc agcactttgg
     4081 caacccggcc ttcgtaaccg tgcgtccaac tgttgaattt cttgttaaac cagccgaaga
     4141 aacctttttt ctcttcgtga tgccctttcg ggattgtctt caacattgtg gcacacaaag
     4201 caggggtaag ggtcagcgca aggaaggcgg aaaatgcgat tgatgacgcc atcgtcaggg
     4261 caaactgttt gtaaatattg cccgtcgccc cgctgaacat cgccaacggt acgaacacgg
     4321 aaatcagaac ggcggtaata ccgatgaccg cgcccgaaat ctgacccatc gcttttttgg
     4381 tcgcttcttt gggcggcaag ccttcacccg ccataatgcg ctcgacgttt tcaaccacca
     4441 caatcgcgtc atcgaccacg atgccgatga ccaaaaccat cgcaaacatg gtcagtacgt
     4501 taatcgacat gcccatataa gagatgaagg cgaaaccgcc caacagcgaa atcggtacga
     4561 cgatggtcgg aatcagcgta taacggatgt tttgcaggaa gagatacatt acgacaaaca
     4621 ccagcaccat cgcttcgatt aaagtatgaa tcactttttc aatcgaaatt tcgacgaatt
     4681 tggaagtatc gtaaggggtt ttccagctca taccctgagg aaagtatttt tccaacgtcg
     4741 ccatgcgttc tttaaccgcc tttgccgtcg ccatcgcatt gccgctgttg gacagcatca
     4801 ccgccatacc ggtggtattt acaccgttca gacgggttga ggaagaatag tcttccatac
     4861 ccagtccgac ccttgccaca tccttcaggt aaacattaga accgtcggta ttggcgcgga
     4921 ggatgacgtt gccgaattct tctgccgtac ccaactgccc ttgcgccgtt acggtagccg
     4981 taaccgtctg tccgcgaacg gcgggaagcg aaccgataga acccgctgaa atctggacgt
     5041 tctgggcgga cagcgcactg ccgatatcgg caaacgacaa attgtagttt tgcagtttct
     5101 taggatcaac ccaaatccgc atcgcgcgtt gcgcgccgaa caggcgtacc tgccccacgc
     5161 cttcgatacg ctgcaactcg ggaacgatat tacgctgcgc gtagtcgttc atctcttcgg
     5221 ttgactgcac atccgacgaa agcatcacaa tcatcaggaa attggaacgc gccttggata
     5281 cggttacgcc gtattgctgg acggttgccg gcagcgtgct caatacttcg gaaagcttgt
     5341 tctgcacttc cacctgcgcc agattctcgt cggtatcggg cgtaaaggtc aggctcacgc
     5401 tgccgctgcc gctcgaatcg gcagaagtgg acagataatc caaaccttcc acgccgttca
     5461 tattccgctc gatcacggaa agcacgctgt cttccattac ctgcgcggac gcgcccggat
     5521 aagtggccct cagggtgatg gtcggggcgg cgacagacgg atattgcgaa acaggcaggc
     5581 ttttaatgcc gaaaataccc gccgcaataa tgaaaatcga aataacccac gcgaaaatgg
     5641 ggcggtcgat aaaaaattta gccatcgatg ccttccttat tccgcttcag aagcagtttt
     5701 ggcttcagat gccgtctgaa cgccggattg aggcgcggcg gcttggtttt cagacggcgc
     5761 ccattctttg ggcgttacct ttttcgcacc cgttataccg gcgatactga tgccttccac
     5821 aaccaccttg tccccgtcct tcagacccga cgtaacaatc caattcgtac cctgctgttg
     5881 cgcgaccgtt acctcgcggg gttccatacc gccttgggcg ttcacaatca tcacggtatc
     5941 tttcgcaccg cgcgttaccg cctgctgcgg cacgatgaat gcgttatcca ccgccacttg
     6001 gtccatcagc acgcgcacat acaggccggg catcaggata ttctgatcgt tcggtacggc
     6061 ggcgcgcagg gtaatctgac cggtcgattc gttgacggtc ggatcggcaa acagcaggcg
     6121 gcctttttca gggtaaactg tgccgtcgtc aaatttgatg ccgaccgcaa tcacaccatc
     6181 cgccgccagc agtttgcctt cggctatctg acggcgcaat ttcatcactt cggatgcaga
     6241 ctgggtaacg ttcacataca tcggattggt ttggcggatg gttgccagca cggtcgtatc
     6301 gcccgcattc aacagcgtac cttcggaaac tttggactga ccgataaagc cggaaatcgg
     6361 cgcggtaatg cgcgaacggt tcagattaat gccggcagat ttgattgccg cctgtgctgc
     6421 tttgacacct gcctcggcag aacgtttcgc cgttaccgca gcatcgtatt cctgccggct
     6481 gacggcttcg gcggcaacca aaggcttgta ccgcgccaaa tccgcatccg ctttggcaag
     6541 cgttgcctga gccgttgcca gttgcgcgcg tgcgctttcc agacctgctt cataagtgga
     6601 actgtcgatc tgatacagcg gctgtccggc acggacataa ctgccttctt ggaacaggcg
     6661 tttttggatg atgccgccga cttgggcgcg gacatcggcg gtacgcagcg attccaaacg
     6721 ccccggcaac tcgacggtca atgcgacggt ttgcggatgg acggttacga caccgacgac
     6781 gggagccggt gcttcccgac cagcaggctg cccgccctgc gccgcgtctc cgcctttacc
     6841 gcaagacgac agtaccaatg caacggcggc agccaacgcg gccgcacgca tcgccttaaa
     6901 agcataaaaa gccattattt atcctatctg tctggtttga tgtaaagggt tttgccaatc
     6961 aacaggcatt
//
LOCUS       DAWEWP010000107         6727 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWEWP010000107 DAWEWP010000000
VERSION     DAWEWP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.107"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="WGN53_001519"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="WGN53_001519"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0140498.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAVEGASLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1102..1512
                     /locus_tag="WGN53_001520"
     CDS             1102..1512
                     /locus_tag="WGN53_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ0140499.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAA
                     DDAEAFGRFMDELIAYLEANDFEMAGGGDCGDFSCLFQKSAPRGLGETEKKAIISWLA
                     GREDVATLHATPLVDGFYGDDGWENYSETYKQAD"
     gene            1533..3596
                     /locus_tag="WGN53_001521"
     CDS             1533..3596
                     /locus_tag="WGN53_001521"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ0140500.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNKTLGHRFLSNGEITIENADSYAAQMREQGKVVA
                     PFAERKAAIQTALERQARRLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTADPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3613..4659
                     /locus_tag="WGN53_001522"
     CDS             3613..4659
                     /locus_tag="WGN53_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0140501.1"
                     /translation="MPSEAFRRHRAYRENKLQSLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTPPAGVWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            4701..5528
                     /locus_tag="WGN53_001523"
     CDS             4701..5528
                     /locus_tag="WGN53_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0140502.1"
                     /translation="MQNHVISLASAAERRAHIADTFGAHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGMPVCQLDPALCAQELHYAKFHDQN
                     SALGSLIEHDRCLNSKQQRRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5540..5635
                     /locus_tag="WGN53_001524"
     CDS             5540..5635
                     /locus_tag="WGN53_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ0140503.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            5677..6519
                     /locus_tag="WGN53_001525"
     CDS             5677..6519
                     /locus_tag="WGN53_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0140504.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1718 a         1792 c         1773 g         1444 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacctt ctgggcagac aaaggctgca
      181 ccgtcatcca acccttcgac atggaagtcg gtgccggcac atcccatccc gccacctgcc
      241 tgcgcgcgct cggtcccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccta
      301 aagacggccg ctacggcgac aatcccaacc gcctgcaaca ctactatcaa ttccaagtcg
      361 ccctcaaacc cgcccccgcc aatatccaag acctctatct tgattctctg cgcgaattgg
      421 gcatcgaccc caaagtccac gacatccgtt ttgtcgaaga cgactgggaa aatccgactt
      481 tgggcgcgtg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctacttcca acaagtcggc ggcattgact gcacccccgt actcggcgaa atcacctacg
      601 gcatcgaacg cttggcgatg tacctgcaag gcgtggaaaa cgtctatgac ctcgtttggg
      661 caaaaacgct ggacggcaat accgtaacct acggcgacgt gtaccaccaa aacgaagtcg
      721 aacaatccac ctacaacttc gaatacagcg atgccgattg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgttgaagg agcaagcctt gcgctgcctg
      841 cctacgagct ggttctcaaa gcggggcata cgttcaacct cttggacgcg cgcggcgcga
      901 tttccgtaac tgagcgggca acttatattg gacgtattcg tgcattgagc cgcgccgtgg
      961 cgcaaaaata tgttgaaagc cgcgaaaaac tgggcttccc tttaatgaag gcaaatgctg
     1021 cctaaggggg cgacgatttt ttacgttcgg gcggcacgat aaggcagccg tccggaagcc
     1081 attatatttt ttaggaaaat catgcctgtc tatttccgca ccccaagcag gaaacgcctg
     1141 cacaaaatga accgccgcca gcataaaaag ctgcatttgg gcgagtttca aacattgatt
     1201 ttcggcgttc aaggcagttt gcaccaagct gcggacgatg ccgaagcgtt tggccgcttt
     1261 atggacgagc tgattgcgta tcttgaggca aacgactttg aaatggcggg cgggggcgat
     1321 tgcggcgatt tttcgtgcct gtttcaaaaa tccgcccccc gaggcttggg cgaaaccgag
     1381 aaaaaagcca ttatctcttg gctggcaggc cgcgaagatg tcgccacttt gcacgccacc
     1441 ccgctggttg acggttttta cggggatgac ggctgggaaa attacagcga aacctataaa
     1501 caggcagatt aaccaaattc aaagaaagcc gaatgatgac ccaaaccctt ttaatcgaac
     1561 tccttaccga agaactcccg cccaaagctc tgaataatct gggcaaccat tttgccgccg
     1621 ccgttgccga aggcttggaa aaagcgcaac tggttgacgg tgcagccgaa tttacggctt
     1681 atgcctcgcc gcgccgtttg gcggttcaag tcaaaaacgt caaagccgtt caggccgacc
     1741 aaaaaatcgt gaaaaaaggc cctgccgtgg cgaatgccat gaaagacggt gcgccgacca
     1801 aggctttgga aggtttcgca cgcggtgcgg gcgcgaaaat cgaagacttg accatcgtcc
     1861 acgacggcaa gcaggacgtg tacgcctacg aatacgtcca aaccggcaaa ccgttgggcg
     1921 agcttttgga agacattatc aatgccgcag tgaaaaaatt gccgattcca aaagtaatgc
     1981 gttggggcag cagcacattt accttcgtgc gccccgtcca cgggctgatt gtgctgcacg
     2041 gcggcgatgt cgtgaacgtc agcgttttgg gtctgcaaag cggcaacaaa accttgggac
     2101 accgcttcct ctccaacggc gaaatcacca ttgaaaacgc cgacagctac gccgcacaaa
     2161 tgcgcgaaca aggcaaagtc gtcgctccgt ttgccgagcg caaagccgcg attcagacgg
     2221 cattggaaag gcaggcgcgc cgtctgaacg ccgccgtcgc cgccgatgaa gccttgttgg
     2281 acgaagtaac cgcgctggtc gaatggcctg tggtattgga agccggtttt gaggaacact
     2341 tcctcgccgt gcctcaagaa tgcttgattt tgacgatgca gcaaaaccaa aaatacttcc
     2401 cgctgctcga ccaaaacggc aagctgatga accgcttcct gctggtctcc aacctgcaaa
     2461 ccgcagaccc gtcgcacatc atccaaggca acgaacgcgt attgcgcgcg cgcctgtctg
     2521 atgccgagtt cttctacaag caagaccaaa aagcgactct ggaaagccgc ctgcccaagc
     2581 tggcaaacgt ggtgtaccac aacaaaatcg gttcgcaggc cgaacgcatc gaacgcctgc
     2641 aaagcattgc cgcccacatc gctaaagcgt tgggcgcaga tgctgccgca gccgaacgcg
     2701 ccgcacgttt ggcaaaagcc gacttggtga ccgaaatggt cggcgagttc cctgaactgc
     2761 aaggcacgat gggcaaatac tatgcccgct tggacggcga aaccgaagaa atcgccgaag
     2821 ccatcgagca gcactatcag ccgcgttttg ccggcgacaa gctgcccgaa agcaaaattg
     2881 ccgccgccgt ggcactggcg gacaagctgg aaaccctggt cggcatttgg ggcatcggtc
     2941 tgattccgac cggcgacaaa gacccctacg ccctgcgccg cgccgccttg ggtattttgc
     3001 ggatgctgat gcagtacggt ttggacgtga acgaactgat tcagacggca ttcgacagct
     3061 tccccaaagg tttgctcaac gaaaaaacgc cgtctgaaac cgccgacttt atgcaggcgc
     3121 gccttgccgt gttgctgcaa aacgattatc cgcaagacat cgttgccgcc gtactcgcca
     3181 aacagccgcg ccgtttggac gatttgaccg ccaaactgca ggccgttgcc gcgttcaaac
     3241 aactgcccga agccgccgcg ctcgccgccg ccaacaaacg cgtgcaaaac ctgctgaaaa
     3301 aagccgatgc cgagttgggc gcggttaacg aaagcctgtt gcaacaggac gaagaaaaag
     3361 ccctttttgc tgctgcgcaa ggcttgcagc cgaaaatcgc cgccgccgtc gccgaaggca
     3421 atttccaaac cgccttgtcc gaactggctt ccgtcaagcc gcaagtcgat gccttctttg
     3481 acggcgtgat ggtgatggct gaagatgccg ccgtgaaaca aaaccgcctg aacctgctga
     3541 accgcttggc agagcagatg aacgcggtag ccgacatcgc gcttttgggc gagtaaccgt
     3601 tgtacagtcc aaatgccgtc tgaagccttc agacggcatc gtgcctatcg ggagaataaa
     3661 ttgcagtctt tagtcagcgt attgatttgc gcctacaacg tagaaaaata tttcgcccaa
     3721 tcattagccg ccgtcgtgaa tcagacttgg cgcaacttgg agattttgat tgtcgatgac
     3781 ggctcgacag acggtacgct tgccattgcc aaggattttc aaaagcggga cagccgtatc
     3841 aaaatccttg cacaagctca aaattccggc ctgattccct ctttaaacat cgggctggac
     3901 gaattggcaa agtcaggaat gggggaatat attgcacgca ccgatgccga cgatattgcc
     3961 gcccccgact ggattgagaa aatcgtgggc gagatggaaa aagaccgcag catcatcgcg
     4021 atgggcgcgt ggctggaagt tttgtcggaa gaaaaggacg gcaaccggct ggcgcggcat
     4081 cacaggcacg gcaaaatttg gaaaaagccg acccgacacg aagatattgc cgactttttc
     4141 cctttcggca accccataca caacaacacg atgattatga ggcgcagcgt cattgacggc
     4201 ggtttgcgtt acaacaccga gcgggattgg gcggaagatt accaattttg gtacgatgtc
     4261 agcaaattgg gcaggctggc ttattatccc gaagccttgg tcaaataccg ccttcacgcc
     4321 aatcaggttt catccaaata cagcatccgc caacacgaaa tcgcgcaagg catccaaaaa
     4381 accgccagaa acgatttttt gcagtctatg ggttttaaaa cccggttcga cagccttgaa
     4441 taccgccaaa taaaagcagt agcgtatgaa ttgctggaga aacatttgcc ggaagaagat
     4501 tttgaacgcg cccgccggtt tttgtaccaa tgcttcaaac ggacggacac gccgcccgcc
     4561 ggcgtgtggc tggattttgc ggcagacggc aggatgcggc ggctgtttac cttgaggcaa
     4621 tacttcggca ttttgcaccg attgctgaaa aaccgttgaa aaacgccgtt ttatccaaca
     4681 gacaaaaaac aggataaatt atgcaaaacc acgttatcag cttggcttcc gccgcagaac
     4741 gcagggcgca cattgccgat accttcggcg cgcacggcat cccgtttcag tttttcgacg
     4801 cactgatgcc gtctgaaagg ctggaacagg caatggcgga actcgtaccc ggcttgtcgg
     4861 cgcatcctta tttgagcgga gtggaaaaag cctgctttat gagccacgcc gtattgtgga
     4921 aacaggcatt ggacgaaggt ctgccgtata tcgccgtatt tgaagatgat gtcttgctcg
     4981 gcgaaggcgc ggagaaattc cttgccgaag acgcttggct gcaagaacgc tttgaccccg
     5041 attccgcctt tatcgtccgc ttggaaacga tgtttatgca cgtcctgacc tcgccctccg
     5101 gcgtggcgga ctactgcggg cgcgccttcc cgctgttgga aagcgaacac tgggggacgg
     5161 cgggctatat catttcccga aaagcgatgc ggtttttcct ggacaggttt gccgccctgc
     5221 cgcccgaagg gctgcacccc gtcgatctga tgatgttcag cgattttttc gacagggaag
     5281 gaatgccggt ttgccagctc gatcccgcct tgtgcgccca agagctgcat tatgccaagt
     5341 ttcacgacca aaacagcgca ttgggcagcc tgatcgaaca cgaccgctgc ctgaacagca
     5401 aacagcaaag gcgcgattcc cccgccaaca cattcaaacg ccgcctgatc cgcgccttga
     5461 ccaaaatcag cagggaaagg gaaaaacgcc ggcaaaggcg cgaacagttc attgtgcctt
     5521 tccaataaaa ggaggagaga tggacatcgt atttgcagca gacggcagga tgcggcggct
     5581 gtttaccttg aggcaatact tcggcatttt gcgccgattg ctgaaaaacc gttgaaaaac
     5641 gccgctttat ccaacagaca aaaaacagga taaattatgc aaaaccatgt tatcagctta
     5701 gcttccgccg cagaacgcag ggcgcacatt gccgatacct tcggcaggca cgacattccg
     5761 tttcagtttt tcgacgcact gatgccgtct gaagaactga atcggatgat ggcggaattg
     5821 gtgccgggat tggcaaaaca gcacctgttg agcgaagtgg aaaaagcctg ctttatgagc
     5881 cacgccgtat tgtggaaaca ggcattggat gaaggtctgc cctatgttgc cgtatttgag
     5941 gatgatgtcc tgcttggcaa agacgcagaa aagttccttg ccgaagatac ttggttggaa
     6001 gagcgttttg ataaggattc tgcctttatc gttcgtttgg aaacgatgtt tgcgaaagtt
     6061 attgtcagac cggataaagt cctgaattat gagaaccggt catttccttt gctggagagc
     6121 gaacattggg ggacggctgg ctatatcatt tcgcgtgagg cgatgcggtt tttcttggaa
     6181 aggtttgccg ttttgccggc agagtggatt aaagcggtag attggatgat gtttacttat
     6241 ttctttgata aggaggggat gcctgtttat caggttaatc ccgccttatg tacccaagaa
     6301 ttgcattatg ccaagttcct cagtaaaaac agtatgttgg gtagcgattt ggaaaaagat
     6361 agggaacaag aaagaagaca ccgccgttcg ttgaaggtga tgtttgactt gaagcgtgct
     6421 ttgggtaaat tcggtaggga aaagaagaaa agaatggagc gtcaaaggca ggcggagctt
     6481 gagaaagctt acggcaggcg ggtcatatcg ttcaaatagt ttgtgtaaaa tatagcggat
     6541 taaaattaga aatggataca ctgtcattcc cgcgcaggcg ggaatctagt ccgttgagtt
     6601 tcagtcattt ccgataaatc accgcaacgt taaatttcta gattcccgcc tgcgcgggaa
     6661 tggcggtggt gggatttctg tttttccgac aaattcctaa aacttaaagt tccgttattc
     6721 ccgcgaa
//
LOCUS       DAWEWP010000108         6487 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWEWP010000108 DAWEWP010000000
VERSION     DAWEWP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.108"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            109..1650
                     /locus_tag="WGN53_001526"
     CDS             109..1650
                     /locus_tag="WGN53_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ0140505.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAAMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGWQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRACSDKAVSPVMPSEPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDGGEGGK
                     RTETL"
     gene            1679..2278
                     /locus_tag="WGN53_001527"
     CDS             1679..2278
                     /locus_tag="WGN53_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ0140506.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2275..2880
                     /gene="nadD"
                     /locus_tag="WGN53_001528"
     CDS             2275..2880
                     /gene="nadD"
                     /locus_tag="WGN53_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ0140507.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLASQGVSDGIPPAAARYIREHGLYEK"
     gene            2939..3325
                     /gene="rsfS"
                     /locus_tag="WGN53_001529"
     CDS             2939..3325
                     /gene="rsfS"
                     /locus_tag="WGN53_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ0140508.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3379..3849
                     /gene="rlmH"
                     /locus_tag="WGN53_001530"
     CDS             3379..3849
                     /gene="rlmH"
                     /locus_tag="WGN53_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ0140509.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYVLKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKTWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            3924..4190
                     /locus_tag="WGN53_001531"
     CDS             3924..4190
                     /locus_tag="WGN53_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ0140510.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4347..5030
                     /locus_tag="WGN53_001532"
     CDS             4347..5030
                     /locus_tag="WGN53_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ0140511.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5154..5666
                     /gene="coaD"
                     /locus_tag="WGN53_001533"
     CDS             5154..5666
                     /gene="coaD"
                     /locus_tag="WGN53_001533"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ0140512.1"
                     /translation="MSPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTIAERRDMLHDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETVKRYVPPAVYQ
                     KMIAEHHNNA"
     gene            5702..6355
                     /locus_tag="WGN53_001534"
     CDS             5702..6355
                     /locus_tag="WGN53_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ0140513.1"
                     /translation="MWEILFRHQDFVAINKPQGISVHRSDGEVSLTATLAAQLGVEKV
                     WLLHRLDKQTGGILLFALNPQSAAVLAAQFAERKMKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNMENIAVTRFFSIRISEKMRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDRLYGGTESETTFLYAWKIQFTYQQQEIEITAPLKNAWQAENISHVLEEFYMAKAG"
BASE COUNT         1422 a         1691 c         1873 g         1501 t
ORIGIN      
        1 ttcagacggc gggaggaagg cggatacggt atgtagtcgg attgaaatat acggtatgtg
       61 gggcttttgg gtacaatcgc aacatcagtg tttcaagtgg ggaaggcgat ggatggacgg
      121 cgttgggcgg tatggggtgc ttttgccctg ctgccttcgg cttttttggc ggcaatggtc
      181 gttgcgcctt tgtgggcggt ggcggcgtat gacggtttgg cgtggcgcgc ggtgctgtcg
      241 gatgcctata tgctcaaacg tttggcgtgg acggtatttc aggcagcggc aacctgtgtg
      301 ctggtgctgc ctttgggcgt gcctgtcgcg tgggtgctgg cgcggctggc gtttccgggg
      361 cgggctttgg tgctgcgcct gctgatgctg ccgtttgtga tgcccacgtt ggtggcgggc
      421 gtgggcgtgc tggctctgtt cggggcggac ggtctgttgt ggcgcggctg gcaggatacg
      481 ccgtatctgt tgttgtacgg caatgtgttt tttaacctgc ccgtgctggt cagggcggcg
      541 tatcaggggt ttgtgcaagt gcctgcggca cggcttcaga cggcacggac gttgggcgcg
      601 ggggcgtggc ggcggttttg ggacattgaa atgcccgttt tgcgcccgtg gcttgccggc
      661 ggcgtgtgcc ttgtcttttt gtattgtttt tccgggttcg ggctggcatt gctgctgggc
      721 ggcagccgtt atgccacagt cgaagtggaa atttaccagt tggtcatgtt cgaactcgat
      781 atggcggttg cttcggtgct ggtgtggctg gtgttggggg taacggcggc ggcagggttg
      841 ctgtatgcgt ggttcggcag acgcgcgtgt tcggataagg cagtttctcc tgtgatgccg
      901 tctgaaccgc agtcggtcgg ggaatatgtg ctgctggcgt ttgcggcggc ggtgttgtct
      961 gtgtgctgcc tgtttccttt gttggcaatt gttgtgaaag cgtggtcggc cggcgaatcg
     1021 tggcgtgtgt taatggaaag tgaaacgtgg caggcggtgt ggaatacttt gcgcttctcg
     1081 gcggcggcgg tgtttgcggc ggcggttttg ggtgtggtgt atgcggcggc ggcgcggcgg
     1141 tcggcgtgga tgcgcgggct gatgtttttg ccgtttatgg tgtcgccggt ttgtgtttcg
     1201 gcgggcgtgc tgctgcttta tccgcagtgg acggcttcgt tgccgttgct gctggcgatg
     1261 tatgcgctgc tggcgtatcc gtttgtggca aaagatgttt tgtcggcttg ggacgcactg
     1321 ccgccggatt acggcagggc ggcggcgggt ttgggcgcaa acggctttca gacggcatgc
     1381 cgcatcacgt tccccctctt gaaaccagcg ttgcggcgtg gtctgacttt ggcggcggca
     1441 acctgcgtgg gcgaatttgc ggcaaccttg ttcctgtcgc gtcccgagtg gcagacgctg
     1501 acgactttga tttatgccta tttgggacgc gcgggtgagg ataattacgc gcgggcgatg
     1561 gtgctgacat tgctgttggc ggcgttcgcg ctgggtattt tcctgctgtt ggacggcggc
     1621 gaaggcggaa aacggacgga aacgttataa tgcgaaccct ttttcagagg acggcaaaat
     1681 gagcgagttg gataacatcc ttgcccataa ccggcagttt gtcgagtcgg gcgaatatga
     1741 aaaatacttt accgacaaat accccgaacg cgggctggca gttttgtcct gtatggatgc
     1801 gcggattatc gggctgctgc ccgacgcgtt gggtttgaaa aacggcgatg ccaagctgat
     1861 taaaaatgcc ggcgcgctgg ttacgcaccc gtggggttcg gtgatgcgga gccttttggt
     1921 tgccgtgttt gaactgaagg tcagagagat tatggtcatc gcccatcacg attgcggtat
     1981 gcaggggctg aatgccgaag aattcctcgg gcgcgtccgg gaaagccgga ttcccgaaga
     2041 ccgtatcgaa accctgcgtt atgccggtat cgacctcgac ggctggctga ccggtttcga
     2101 caacgtcgaa gacagcgtgc gccacacggt ggaccttatc cgtaaccatc cgctgatgcc
     2161 gcgccatatc gccgttcacg gattggtcat ccatcccgtt accggcaaac tgacgctggt
     2221 tgtggacggc agtgtttcag acggcatgga cttatcggaa ggaatggaaa catcatgaag
     2281 aaaatcggac tgttcggcgg cactttcgac ccgatacaca acgggcatct ccacatcgcc
     2341 cgcgcctttg ccgacgaaat cggcttggac gcggttgttt tcctgccggc aggcggcccg
     2401 tatcacaaag acgcagcctc cgcttccgcc gccgaccgcc ttgccatggt cgaactggcg
     2461 acggcagaag acgcgcgttt tgccgtcagc gattgcgaca tcgtccgaga aggtgcaacc
     2521 tatacttttg ataccgtcca aatcttccgc cagcagttcc catccgcgca actctggtgg
     2581 ctgatgggca gcgacagcct gatgaagctg cacacatgga aaaaatggca gatgctcgtg
     2641 cgcgaaacca atatcgccgt cgccatgagg cagggcgaca gcctgcacca aaccccgcgc
     2701 gaactgcacg cgtggctggg caagtccctt caggacggca gcgtccgcat cttgtccgcc
     2761 ccgatgcata atgtgtcgtc aacggaaatc cgccgcaacc ttgccagcca aggcgtttca
     2821 gacggcatcc cgcctgccgc cgcacgctac atccgcgaac acggtttgta tgaaaaataa
     2881 agtcaaatca gtaagaaacg gctataatgc cgtctgaaaa catcccgtta ggaaaataat
     2941 gaacgaacaa gaactgcaag acctgcaaaa aatggtcggg gtcgccgtca acgccctcga
     3001 agacatcaaa gccaaagaca tttccgttct cgaaacgcaa gacaaaactt cgctgtttgc
     3061 cagaatgatt atcgccagcg gcgacagtac gcgccaagtc aaagcactgg ccaacaacgt
     3121 tgccgtcgat ttgaaagaag ccggttttga aatcctcagc accgaaggcg acagcggcga
     3181 atggacgttg gttgatgcag gagacctcgt cgtccacgtt atgctccctg ccgtgcgcga
     3241 cttctatgat atcgacaccc tttggggcgg cgagaaaccg agtttccacg ccggaatgca
     3301 gaagccgtgg cacgcggcag actgattccc gatgccgtct gaatgttcag acggcatttt
     3361 cctgtaaggg agaaagcatt gaacatcacc gttttggcag tcggcaccaa aatgccgcgc
     3421 tgggttgatg aggccgtcgc cgaatacgcc aaacgcttcg gacgcgacgt cgcctacgta
     3481 ctcaaagaaa tcaaacccga aaaacgcggc gcgggcgtga atgccgccca aggaatggcg
     3541 gcggaagaaa aacgcatcct cgaagccata ccgcaaggcg cgttcctcgt cgtccttgac
     3601 gaacgcggca aagcaccgac ctccgtcgag ctggcggaac acctcaaaac ttggcagcaa
     3661 aacggcgaac acgtctgctt cgtcatcggc ggcgcggacg gcatgaccga ccgcctcaaa
     3721 cagcaggccc gcatgatgat gcgcctgtcc agcctgaccc tgccgcacgg gatggtgcgc
     3781 gtccttctga ccgaacaact ctaccgcgcc gcctccatcc tgcacaacca cccctaccat
     3841 cgggaataag aaggcttttg tccgcactcc caatcggtta aaatcccccc gttatttctg
     3901 aacacacagc aaaggaatcc gctatggcac gcatggtatt ctgcgtcaag ctcaacaaag
     3961 aagccgaagg catgaaattt ccgccgcttc ccaacgaatt gggcaagcgc atttttgaaa
     4021 acgtatcgca agaagcgtgg gcggcgtgga cgcgccacca aacgatgctg attaacgaaa
     4081 accgtttaag cctcgccgat ccgcgcgcgc gcgaatacct ggctcagcag atggagcagt
     4141 atttcttcgg cgacggcgcg gatgccgttc agggatacgt tccgcaataa cggttttccg
     4201 ttttgaacac aggctgtccg aaactgcttc agacggcctt taaaatacgc cggacaactt
     4261 tatcttcacg caaccggaca aattttgaca ttgggcaatg ttaaaatccc ccattattcc
     4321 caaccgcgct ttcaggagca gatgatatgc aacacgacgt ttacgactac accgcgcata
     4381 cggtttctaa aaacaccgtc ctgcagaaaa cctaccgcct gctcggattt tcattcattc
     4441 cggcagccgc aggcgcggca cttgccgcca atgccggttt caatttttac gccgccttcg
     4501 gttcgcgctg gatagggttt gccgtcgtgt tggcgttttt ctacggtatg atccacttca
     4561 tcgagaaaaa ccgttacagc aataccggcg ttaccctgct gatggtattc acattcggta
     4621 tgggcgtatt gatcggcccc gtgctgcaat acgcgctcca tattgccgac ggtgcgaaaa
     4681 tcgtcggcat tgccgccgcg atgaccgccg ccgtcttttt aacgatgtcc gccttggcgc
     4741 gccgaacccg gctcgatatg aacgcgctcg gacgcttcct gaccgtaggt gcggtcattc
     4801 tgatggtcgc cgtggttgcc aatctgtttt tgggtattcc cgcactcgcc ctgaccattt
     4861 ccgccggttt tgtcttgttc agttccttaa tgattatgtg gcaggtacgc accgtcatcg
     4921 acggcggcga agacagccac atcagcgcgg cactgacact gtttatctcg ctttacaaca
     4981 tcttcagcag cctgctgaac atcctgttgt ctttaaacgg cgaggattga tgccggtaaa
     5041 acgccgtccg gctatgccgt ctgaaaatgc ttcagacggc attttacttt gggcatacaa
     5101 tacggatgca cacgcaccaa cacacagccg gctatgaggc cggaaggaat catatgtcac
     5161 cgaatacacc gcgccgcgcc gtttatgccg gcagtttcga tccgcccaca ttggggcatc
     5221 tgtggatgat acggcaggcg caatctatgt ttgacgaact catcgtcgcc atcggcatta
     5281 atcccgacaa acgcagcacc tacacgatag ccgaaaggcg ggatatgctg cacgacatta
     5341 ctaaaatgtt tcccaacgtc agaaccgatg tatttgaaaa ccggtttttg gtgcattacg
     5401 cccgtgaggt agatgcagga ttcatcgtgc gcggcatccg ttccgcctcc gattatgaat
     5461 atgaacgctc catgcgccat atcaacagcg acctcgcacc cgaaatatcc accgtattcc
     5521 ttatgccgcc gcgcgaaatt gccgaagtgt cgtccactat ggtcaaagga ctggtcgggc
     5581 cggaaggctg gacggaaact gtcaaaagat atgtgccgcc ggccgtgtat caaaaaatga
     5641 ttgcagaaca tcacaacaat gcctgacaga aagcttctgt caaatatatt caggttcaaa
     5701 tatgtgggaa atcttattca gacaccaaga ttttgtcgcc atcaataaac ctcaaggcat
     5761 atccgtccac cgttccgacg gcgaagtcag tctgacggca acacttgcgg cacagttggg
     5821 cgtagaaaag gtatggttgc tgcacaggtt ggacaagcag accggaggca tattgctgtt
     5881 tgcccttaat ccccaaagtg ccgctgttct tgccgcacag tttgccgaaa ggaaaatgaa
     5941 gaaaacctat ttggctttgt ccgaccgcaa accgtccaaa aaacaaggat ggataaaagg
     6001 cggcatggaa aaatccagac gcggaatgtg gaagctgacg cgcaatatgg aaaatattgc
     6061 cgttacccgg tttttcagta tacgtatcag tgaaaaaatg cggctgttca ttcttgagcc
     6121 tcataccggt aagacacatc agctgagagt ggcaatgaaa agtctgggta gtcctatttt
     6181 gggggaccgc ttatatggag ggacagaatc cgaaactacg ttcctgtatg catggaaaat
     6241 acaatttacc tatcaacaac aagaaatcga aattactgca cctttgaaaa atgcatggca
     6301 ggctgaaaat atctctcatg tgctggaaga attttatatg gcaaaagcag gttaataaat
     6361 ataaattaat ttataaacaa tatgataaat atttttataa aaatttttca agaaaacggt
     6421 tgaccgcaaa tctcttcaag cgtatagttc gcttcttcgc tgctgacgaa acggcggtga
     6481 tgaagta
//
LOCUS       DAWEWP010000109         6485 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWEWP010000109 DAWEWP010000000
VERSION     DAWEWP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.109"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            16..342
                     /locus_tag="WGN53_001535"
     CDS             16..342
                     /locus_tag="WGN53_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0140514.1"
                     /translation="MGEKIKDGKNQKTVLSREEEQKICNTFTHKQAVEDFSVVIGYDE
                     IKAKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKDRLA
                     MLKFNS"
     gene            353..490
                     /locus_tag="WGN53_001536"
     CDS             353..490
                     /locus_tag="WGN53_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0140515.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYR
                     K"
     gene            complement(650..1987)
                     /gene="ribB"
                     /locus_tag="WGN53_001537"
     CDS             complement(650..1987)
                     /gene="ribB"
                     /locus_tag="WGN53_001537"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ0140516.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHNLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPAADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2208..3223)
                     /locus_tag="WGN53_001538"
                     /pseudo
     CDS             complement(2208..3223)
                     /locus_tag="WGN53_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(3391..3984)
                     /gene="ribA"
                     /locus_tag="WGN53_001539"
     CDS             complement(3391..3984)
                     /gene="ribA"
                     /locus_tag="WGN53_001539"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ0140517.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3977..4654)
                     /locus_tag="WGN53_001540"
     CDS             complement(3977..4654)
                     /locus_tag="WGN53_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140518.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVQSAAVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADEAAQAEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            4808..4892
                     /locus_tag="WGN53_001541"
     tRNA            4808..4892
                     /locus_tag="WGN53_001541"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4842..4844,aa:Leu,seq:tag)
     gene            5413..6447
                     /gene="purM"
                     /locus_tag="WGN53_001542"
     CDS             5413..6447
                     /gene="purM"
                     /locus_tag="WGN53_001542"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ0140519.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGELDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQDLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         1605 a         1592 c         1692 g         1596 t
ORIGIN      
        1 atcgacgcat cgggcttggg cgaaaaaatc aaagacggca aaaaccaaaa aaccgtactt
       61 tctcgcgaag aagaacaaaa aatctgcaat actttcacgc acaaacaagc agtggaagat
      121 ttcagcgtgg tgatcggcta cgatgaaatc aaagcgaaga atcacagctt gtcggcgggg
      181 cagtatttcg aggtaaagat tgattatgtg gatatttccg ccgacgaatt tgcgcaaaaa
      241 atggcgggat tttcagcgga tttggataaa cttttcgccg aatctgccga attggaaaaa
      301 gagattaagg atagattggc gatgttgaaa ttcaattcat aaataccgat gaatgaaacc
      361 ggtcaaactt aaaatgccgt ctgaaagcct ttcagacggc attttgtttg gtttggaaaa
      421 tacttatgcc gttctttccc tgaaagagag aatccaaaaa accaaagcca caggaattta
      481 tcggaaatga caaaaacccg acgaaccgga ttcccgcctg cgcgggaatg acgaattaga
      541 agttacccga aatttgcaaa aacaaaatcc aacccaacag accgggtttt cgtttgcacg
      601 gaaataatgc aataaataaa gcaaatataa agtatttgaa tttactatat tatttttccg
      661 cttcttcaaa gccgacgact tccaaaccga agccggtcag accggtgaaa gatgagggct
      721 gcccgaggac gcgcagtttt ttgacgttga ggccgacgag gatttgtgcg ccgatgccgt
      781 agcttttgct gtcccatttg taggcttggt ttgcgccttt gggtagggtg cggtcgagca
      841 gggatgcgcc gtcttcggtg cggtgcagaa ggatgacgac gccgctttct gcctgttgga
      901 cgcgctcgag tgctttgggc aatgaccatg aatggcgcgg gttggcttga atgaagtcca
      961 tcacactgaa gggttcgtgg acgcggacga gggtttcggt gtcggcggcg ggcgtgcctt
     1021 tgacgagggc gaggtgggtt tcgccggaga gtttgtcgac gtaaacgtgt tgttggaact
     1081 cgccccacgg ggtttgtaca ggcgcattgc ccatgtcttc gagcaggctt tcggtacggc
     1141 tgcggtattc gatgaggtcg gcaatggtgc cgattttaag gttgtgttct tcggcaaact
     1201 tcatcagctc gggcatacgc gccatggtgc cgtcgtcgtt gatgatttca cagatgacgg
     1261 cggcaggaat cagcccgttc atttgcgcca agtcgacgcc ggcttcggtg tgtccggcgc
     1321 ggacgagtac gccgcctttt tgggcgcgaa gcgggaagat atgaccgggt tggacgatgt
     1381 ctgcgggttt ggcggtcggg gaaacggcgg tttgaatagt cagggcgcgg tcggcggcgg
     1441 aaatgccggt ggtaatgccg tgtgcggctt cgatggagac ggtaaagttg gtgccgtatt
     1501 gcgcgccgtt tttttgggtc atcatcggca gcccgagttt ttcgaccatt tcgccgtcca
     1561 tcggcaggca gaccaagccg cgcgcgtgtt tgatcatgaa gttgatggct tcgggcgtga
     1621 cgaattgcgc cgccatcagc aggtcgcctt cgttttctcg gtcttcggca tcggtgatga
     1681 tgaccatttt gccggctttg atgtcggcaa ggatttcggg gatgggggaa atatgggaca
     1741 tggtgtgttt tcctgtgtgt tcgggcgggc gggcggtgtg ttcggtatcg agccagaagg
     1801 cgggcatttt tgccgcctgt tcgattttac gggctttttt ctcgccgaag gatttgtttt
     1861 tcaaaagggc ggagagttcg cctgtgttga gggcaacggc ttcggcaaaa cgggtttgca
     1921 aaccgccgta gtgttctgct atccattgcc gcaggttgcg gcggcgcaga ccggctgtgt
     1981 cggtcatcgt ttcatccttt gtagaaatcg gatgtagcgg attgtaatcg cgtatttacc
     2041 aaaaagcaaa tctgtttttt cgccaaaccg aattatttgc tttttggtaa acagatgccg
     2101 tctgaagccg gattcgtggc ttcagacggc attgccgcaa tccttgccgg ccgggggctg
     2161 tttgtcccaa cttcccgcgc acttctgatt tggggtttgc cggaagctta ttccgaaaaa
     2221 aatttctgat aaacggcctt gattgccggg tacagcatag gcggtacgag caggcgcaat
     2281 atcggcaatc ctatgatgaa tgcggtcttc ccgataaaaa ccggacggat tgccttcgcg
     2341 ccgaatatct ttctgcattt ttctttaaat gaaaaatatt tccggtagat gaggataatg
     2401 tttttggaaa aataaagcct taccaatata gacctcagat agccgatatt gtcgaagaca
     2461 aatttttgca aaccgccgta gatgggggaa tttttgtttt cgactaaaaa atccacaccc
     2521 cggtcgagat gcgtcagcac atcgctgaaa tttttgtatt tgatgttgtg gctgatagag
     2581 ccgccgagaa cgcggtactg gtaaaaaggg ttatcgtaaa aggcaaaagt cttgataaaa
     2641 cgcgccaatt gcaaactgta cgggaaatcc tcgtgaatgt atccttttgg gaaaaacaga
     2701 ttgtttttga tgattatttc ccgcctgacg atttttgtcc acgcgttggc gatatagtac
     2761 cgcccctcca ccagcgtttc aaaatggcgg acaaaatcat tatccgaaaa gtccgcccct
     2821 ttggggatgt cgcggtaatt gaaggacgag ggatgcagga tcaaatcaac ctttttgtct
     2881 gcaagttgtt gtaaatcaaa gagaatcccc ccccccgcgc gttttttgaa cgattggtgt
     2941 cggcccaata gtcgtcgctg tccaaaaaga ttaggtaatc gccttttgcc gcccggatac
     3001 cggcgttgcg ggcatccgac agcccgccgt tttcttgatg aatcactttt atatgcggat
     3061 atttgcctgc atattcgtcg caaatcttcc cgcagccgtc cggcgaaccg tcatcgacca
     3121 aaatcatttc ataatcggca aaattttcgg caagcacgga atccacgcag cagcggaggt
     3181 atttttccac attgtaaata gggacgatga tggagaaaat cataaatatc aatccattat
     3241 attaagatgt ttgcgcgtat gcctcaaacc cgcacccgca atgcgtttgc atccgcaccc
     3301 tgtaacttta taaaacttat tgctttatca aggtatggaa acctgtttcc cgaaaggcgg
     3361 cgcaggatgc ccgttccctg catctttgcc ttattccgac atcagatgcc ccagcttgtc
     3421 tgctttggtt tggaggtagc gtttgttttc aaggttttcc ccgacgtgca gggggatgcg
     3481 ttcgaccacg ttaatccccg catctttcag ggtttggatt ttttcggggt tgttggtcag
     3541 cagtttgacc gagcggatgc ccagatattc gtagatagat tgcgccaaac ggaaatcgcg
     3601 ggcatcgacg ggcagcccga gtgccaaatt ggcttcaacc gtatccatac cttggtcttg
     3661 cagatgatag gcgcggattt tgttaatcag cccgatgccg cgtccttcct gacgcagata
     3721 gacgatgatg ccgcgcccct ctgtctgtac cgccctcatg gccgcttcaa gttgaggtcc
     3781 gcagtcgcat tttctcgaga acagcgcatc gcccgtcagg cattcggagt ggatgcgcgt
     3841 cagaaccgga ttgccgtctg aaaaattacc gacggtcagc gcgacgtgtt cctgcccgtt
     3901 tgcctcttca aagccgtgca tcgtaaatac gccccattcg gtcggcaggc ggcaggatgc
     3961 cacatggttc aacatttcag acatcttcac tcccatcttt tccggcagac atacccaaca
     4021 gcagtttcaa aggttcggac aaagccaagg caagagccac ccattccgcc tgcgctgcct
     4081 catccgcaca ctctgcgcat tcaaattcca catgaaccac gcccaacaca ccgccacttt
     4141 ccgtgcagac cggaatggaa atttgcgccg ccgaagcatg attgcgttct cccgaaagct
     4201 cccccaaatc caaccaacgg cgtacatccg aggcaacatt catccaaccg ctttgcgccg
     4261 aacggcaagc cagcgaaacc ttgcctgccg cttcatccaa atcccacagg ttttccaaaa
     4321 cctcgccctg tcgggacagg catatcagtt ggaaagcctg gttttcagat ggcatcaagg
     4381 catagaccgc cgcactctgc acgcctgtcg agcgcgaaaa caccgaatcc aaagccatga
     4441 aaagccgttt cagtgcatct tcgcggacat tgtcgcacga caggtaatcg gcaagtttcc
     4501 aaccctcatc actgcgccac aaaaccgaac ggtctatcga agccgtcccc atatccatta
     4561 ccgtctgcgc cgtcagatac gccgcccgaa cctcgtcaag cggcagcttc aaaccctgag
     4621 tcagcagaaa atccttaatc aaagcagcag gcatacccaa attccgtcaa taaaaacaaa
     4681 aaaccgcccg attccgatgt caggcggacg aaaaacagat tttaccgccc caaaccccaa
     4741 gccatcaaca gccccgccga aaaccttgca caaaaaaaca aaatctgtat aatcgtccaa
     4801 ttcccatgcg gacgtggcga aattggtaga cgcaccagat ttaggttctg gcgccgagag
     4861 gtgtgagagt tcgagtctct ccgtccgcac caataatgat aaataaatta tatagttaat
     4921 gcttatcggg acaaattagt gccacaaaac ataagtcgtc tgcttttcag cgggcggctt
     4981 ttttatctgc ctatatgttt caacacgcag aacggtacat aaagtgccgc catttgttcc
     5041 gcaccgaatc ggaaacgggt gcttccgccc gcaatcagat tcaccgcccc taatgccggg
     5101 gtttcaaccg gaaagaagaa aatattgatt ttgatgatgg catcaaaaac ccccttccgc
     5161 tcgtctgcca aaaggaatgg ttaagaaaag caatgcgtct ttatgatgga acgcgatata
     5221 taaggaattg agggaaaggg caggcgggtt tctgcccgtt aatcctgcgg acggtcccgc
     5281 caaacccgac acgcgcgggc atatggcgga ctgtttacaa tattgtctaa aaatctgaac
     5341 ctgcaaacgt gaaataaggt agaatacgcc ccgttatttt accgtccaat ccccatttac
     5401 caaggaaaaa cgatgagtac ttcattgagt taccgcgatg caggtgtcga tatcgacgca
     5461 ggcgaccaac tggtcgaaaa catcaaaccg tttgccaaac gcaccatgcg tccggaagta
     5521 ttgggggatt tgggcggttt tggcgcattg gtcgaaatcg gcaagaaata taaaaatccc
     5581 gtattggtca gcggtacgga cggcgtgggt accaagctca aacttgcctt tgattgggat
     5641 aaacacgata cggtgggcat cgaccttgtt gcaatgagtg tcaacgatat tttggttcaa
     5701 ggagcagaac ctctgttttt cttggattat ttcgcctgcg gcgaattgga tgttccgcgc
     5761 gcaaccgatg tcatcaaagg cattgcacaa ggttgcgaag aatccggctg cgccctgatt
     5821 ggcggggaaa ctgccgaaat gccgggtatg tatcccgtcg gagaatacga tttggcgggt
     5881 tttgccgtcg gcgtagtgga aaaagagaat gtcattaccg gccgcagcat cggcgcgggc
     5941 gatgtggtat tgggtttggc ttccaacggc gcacattcaa acggctattc ccttatccgt
     6001 aaaatcatcg aacgcgacaa tcccgatttg gatgccgagt ttgataatgg caaaaccttg
     6061 cgtgaggctg ttattgcgcc gacccgtctg tatgtgaaac cgattcttgc cgctttggaa
     6121 aaatttacca ttaaaggtat ggcacacatt accggcggcg gcattaccga aaacgtgccg
     6181 cgcgtgttgc ctgaaaacac ggttgcacaa atcgatgcta aatcgtggga attgcccaag
     6241 ctcttccaat ggcttcaaaa ggcgggcaat gtggaaaccc aagaaatgta ccgaaccttt
     6301 aactgcggca tcggcatggt cgttattgtt gctgccgaag atgccgatgc ggttcaggat
     6361 ctcttgggtg aacaaggtga aacagtttac cgtttaggtt tgatccgtga gcgtcaggga
     6421 gacgagcatc aaacccaggt tgcctgattg cttctatagc gaaatgccgt ctcaaacatg
     6481 ggttt
//
LOCUS       DAWEWP010000110         6417 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWEWP010000110 DAWEWP010000000
VERSION     DAWEWP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.110"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            176..1234
                     /gene="alr"
                     /locus_tag="WGN53_001543"
     CDS             176..1234
                     /gene="alr"
                     /locus_tag="WGN53_001543"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ0140520.1"
                     /translation="MRPLNVQIRLGNLRHNYQILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLIRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1298..2653)
                     /locus_tag="WGN53_001544"
     CDS             complement(1298..2653)
                     /locus_tag="WGN53_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ0140521.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAASKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(2669..2941)
                     /locus_tag="WGN53_001545"
     CDS             complement(2669..2941)
                     /locus_tag="WGN53_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ0140522.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3067..3804)
                     /locus_tag="WGN53_001546"
     CDS             complement(3067..3804)
                     /locus_tag="WGN53_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ0140523.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4085..4984)
                     /gene="prmB"
                     /locus_tag="WGN53_001547"
     CDS             complement(4085..4984)
                     /gene="prmB"
                     /locus_tag="WGN53_001547"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0140524.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5129..5722
                     /locus_tag="WGN53_001548"
     CDS             5129..5722
                     /locus_tag="WGN53_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140525.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNKSLNIGVIVREFPDDHKHYDHFILKDE"
     gene            5834..>6417
                     /locus_tag="WGN53_001549"
     CDS             5834..>6417
                     /locus_tag="WGN53_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ0140526.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANPDAVSTCFKADKGAETVNNDTEHNAT
                     YFSHFPSKQKSTDGGRCPFLYRFPYFLSAGRAGFA"
BASE COUNT         1719 a         1620 c         1499 g         1579 t
ORIGIN      
        1 atttgggaaa gcgattatat caggaaaagc aaaccgcctt cctacctgaa aactgctgct
       61 tcggcttgaa gacacaaggg tctttaatat tttaaaagcc ttgccgttgg attataattc
      121 cctaattgat ttcttaattt tgctaataaa cacttgtttg gtaaggaatg aatttatgcg
      181 tcctttgaac gtgcagatca ggttgggcaa ccttaggcac aattatcaga ttttaaagga
      241 aatgcacgga ggcaagctgt tggcggtagt gaaggccgac gcatacggac acggtgcggt
      301 cagatgtgct ttcgcgctgg cagacttggc agacggcttt gccgtggcga caatcgatga
      361 aggaatcagg ctgcgggaga gcggcattac ccatccgatt gtccttttgg aaggcgtatt
      421 tgaagcatca gagtacgaag cggtcgaaca atactcgctt tggccggcag tcggaaacca
      481 atggcagctt gaggctttgc tgatccgcca ttggaaaaaa cccgtcaaag tctggttgaa
      541 aatggattcg gggatgcacc gtaccggttt tttccctcat gattacgctt cggcatatgc
      601 ggcattgaaa cagtcggaat atgtggacag tattgtcaaa ttctcgcatt tctcctgtgc
      661 ggacgaaccc gaaagcggta tgacggaaat acagatggaa gcattcgatt tgggtacgga
      721 agggctggaa ggcgaagaaa gccttgccaa ttcggcggct attttgaatg ttcccgaagc
      781 acgcagggat tgggggcgcg cgggcttggc gttatacggc atttccccgt tcggaggcag
      841 cgatgacagg ctgaaacccg tgatgaggct ttcaacccgt atttttggcg aacgcgtttt
      901 acagccgcac tcccctatcg gttatggcgc aacattttat accagcaaat ctacgcgcgt
      961 cggtctgatt gcctgcggtt atgcggacgg ttatccgcgc cgcgccccaa gcaattcccc
     1021 cgtcgctgtc gacggcaaat tgacccgggt catcggcagg gtctctatgg atatgatgac
     1081 catcgagctg gacgcttcgc aagaaggttt gggacacgag gtcgaactgt ggggcgatac
     1141 ggtcaacatc aatactgttg ccgaagcggc cggaaccatc ccttacgaat tgatgtgcaa
     1201 tatcaaacgt gcaaaattca cttatatcga gtaatcaagt ccaaacaaaa atgccgtctg
     1261 aagcctttca gacggcattt ccccatcaaa accgcaatca gtttttcatc gattgaaccg
     1321 gtgcgggaat cctgccgccc cggttgacga acacttcgca tgagccttct tttaccggca
     1381 ttacaggcgc gtagcccaac aggccgccga actcgacgct gtcgccgacg gtttttccgg
     1441 ttaccggaat aatgcgcacg gcagtggttt tgctgttgat catgccgatg gcggcttcgt
     1501 cggcgatgat gccggaaatg gtgtgtgcgg gcgtatcgcc agggacggca atcatgtcca
     1561 gcccgaccga gcagacggcg gtcatcgctt cgagtttatc caacgtcagc acgcctgctt
     1621 cggcggcggc aatcatacct tcgtcttcgg aaacggggat aaacgcgcca ctcaaaccgc
     1681 caaccgcgct cgaagccatc atgccgccct ttttcacggc atcgttcagc aatgccaaag
     1741 ctgctgttgt gccgtgcgta ccgcagacgc tcaaacccat ttcttcaaga atgcgcgcca
     1801 ccgagtcgcc gacggcaggg gtcggtgcca gcgacaagtc gagaatacca aacgggatat
     1861 tcagcatttt tgaggcttcg cggccgatga gttcgcccac gcgggtaatt ttgaaggcgg
     1921 ttttcttcac aacttcggca acttcggtca atgtcgttgc atccgaattt tccaacgcgg
     1981 cttttacgac acctgggccg gatacgccga cattaatcac agcatccgct tcgcccgatc
     2041 cgtgaaacgc acccgccata aacggattgt cttccaccgc attgcagaac acgacgattt
     2101 tcgcgcagcc gaaaccttcg ggcgtaattt cagccgtgcg tttgatggtt tcgcctgcca
     2161 gcttgaccgc atccatattg ataccggcgc gcgtactgcc gacattgatg gagctgcaca
     2221 cgatgtcggt ggtcttcatc gcttcgggaa tggaacggat taacacctcg tctgaaggcg
     2281 acatgccttt ttgcaccaac gcggaaaagc cgccgataaa ggacacgccg atggctttgg
     2341 cagccttatc caaagtttgc gccacgctga cgtaagaatc ggctttgctc gccgccgcga
     2401 tttgggcaat cggcgtaacg gaaatgcgct gattcacaat cggcacgccg tatttggcag
     2461 acagatattt tgccgtcgcc accaagtctt tgccgaccgt ggtgattttg ttgtaaatgt
     2521 tttggtttaa cacgtcgatg tcggtgctga tgcagtcgtg caaatcaatg ccgatggtaa
     2581 tggtgcgcac gtcaaaattc tggtcggcca ccattttgac ggtttctaaa atctcgccgg
     2641 attgaatact catggctctc cctccgactt aaatacggtg catggcttgg aaaatttctt
     2701 cgttttgcat acggatatca agcgcgagtt ttttgctctc ttccgcaaac aaatccaaaa
     2761 cctcctgccg agatttgggg catttcgagg tatccaccaa gataatcatg gtaaaaaaat
     2821 cgtccatcag ctgttggctg atattaagga tgttgattcg gttttccgcc aaaattttgg
     2881 aaacatcgta cacgatgccg acgcggtctt tgccgatgac ggtgatgact gaattgttca
     2941 caggcttact ccttgcagat atccgttaaa gtccgaaatt ataccaccgt tggattttga
     3001 agaaatattg tcaacaatat atacatacaa aatgccgtct gaaactattt cagacagcat
     3061 caagattcag ggttcgatta aataaccatc cttatcccac tgggttttcc tgaccaactt
     3121 gtcatcctga taaacagcct cgctcttttt agaaccatct tcataccact ccaaaaccac
     3181 cccgttgcgt tgatggtggc ggatagacag ttccgagagt aatcggccgc tttcatccca
     3241 agtcagaatt ttggcaggct catcgttgac cataaccatt tccgttttga tattgccgtc
     3301 ggcataccat tgcttccata cgccgtttgc cttattctgt ttaaactgga tttcgctttc
     3361 cttgccgccg ttacggtaat agcggtaccc cgtaccttca ctcaaaccat ttttataagg
     3421 cataacggca gattttttac cgttcggata ccagttgacc cactccccgt ccggcttacc
     3481 cttgctgaag ccccccgcca tttttttctg accattaaaa tgccacaaaa tcaacatacc
     3541 gttttgcagg gtaggcacaa aagatttgat ttgcgttgaa gcaacgatat aaggttcgga
     3601 atatttcttc atcgacggat aataaaaatc ctgcgcgtgc gcaataccct ccgccacact
     3661 atattgcctg atataagcgg cagaagacat cgtcgccgtc agtttcccgt tctgattaaa
     3721 ataaacagaa taggtctgcg ccggcaaagc ggccgaaaaa cccaacagga cagttgaaaa
     3781 tacaatccga gataattttt tcattgcaat agcgatataa aaacaaggct gtgttttagt
     3841 aatcttttga tttcaattat ttgcaaggga aaagacaatt attttccggt taggaataaa
     3901 cctattctat tgaatatatt gaagccaagt acgcctatca acactatatt aaaacactgc
     3961 caaaaacaat taacttataa acaatatggt aaggatttct ctgccaagca tcaaacccga
     4021 gacaacgtat cgtaaaaatg ccgtctgaaa acaaatcgtc ttcagacggc atttcccctt
     4081 caactcactc ttcacccaat aactgctcgc gcgtcaagag gaaaacaaaa ccgtcgcccc
     4141 cgctggtttc caaccaagta aaaggcaact ccggatacgc tgcttccaat acatccctgt
     4201 tatgcccgat ttccaccagc aatacacctt tgggattcag aaactttgcc gcattcagaa
     4261 gaatctgcct ggtggcatcc aacccgtccg ccccgctgcc caatgccaat tccggttcgt
     4321 gcaaatactc ttcaggcaat aactcaaccg attccgcatc cacataaggc ggattggaaa
     4381 caatcaaatc ataagtgcct tccaatcctt caaacaaatc cgtatgaata agccggatgc
     4441 gttcttccaa accataatct tcgacattaa tccctgccac ttccaaagca tccaagctca
     4501 catcaaccgc atcaatttgg gcatcaggat aatgatgcgc catctgaatg gcaaggcaac
     4561 cgcttccggt gcaaagatcc aaagcattat gcaccagctc atcgtattct atccaaggac
     4621 gaagtccgtt acccaacaat tcataaataa aagaacgagg tatgattacg cgctcatcca
     4681 catagaaatc aaactctccc tgccatgcct ggtgtgtcaa ataagcggct ggaatgtgtt
     4741 cgacagcacg acgctcaata accgccagca cttcctcttt ttcagcttcc aagagttttg
     4801 catcaagata tggggcaagc atatccaaag gcaaattcaa agtatgcaga atcaaataag
     4861 ccgcttcatc atgcgcatta tctgttccat gaccaaaaaa gagccctgcc tcattaaaac
     4921 ggctgactgc aaaacgtaaa atatcgcgga tagtcgtcaa ttcttgtgct gcctgattaa
     4981 acataatatg aaccattctg cgtatagata cttttaatta taacagaaac aacaagcaaa
     5041 ccttttcata tcgccaaata accacccaat ctacccatac aactacataa atgcccgcgc
     5101 gaaaaccatc gcccgaacgg aaacgacaat ggccgacggt atgggcaatc tgattggctg
     5161 ggaaaaaacg gggcttgttg tcggtaagca gtggataacc gcaaaagacg acaaggtgtc
     5221 cgatgtctgc aatgccaacg gcgagatggg cgtaatcggg ctttacgagc ctttctcaca
     5281 cggcgcattg acgatacccg gtcatccgaa ctgccgatgc gaggttgttt ccgtatcggg
     5341 tggcgaattg ggggaatttg ccgaaaaaaa ggagcttcgt aaagcggcta tgcagtatgc
     5401 gcgggataac tttatcggca aaagctatgt caatgaaaac agcgggcatg aactgaaggt
     5461 aacttggcaa ggtgtgaaac acgctgcgtc aaaggcaaat caggcggaat tatccatcat
     5521 gacaaaactt gatgacttat tgcgctacgc aaaatatgag ggttcttatt cggataggaa
     5581 aggtcatcct aatattattg cagcacataa gtatcgtgcc gttgccaagg ttgggaataa
     5641 gtctttaaat atcggtgtga ttgtaaggga atttccagac gaccataaac attacgacca
     5701 tttcatcttg aaggatgaat aaagcccttt tgcagtgtcg ttctggagcg gatagcgtta
     5761 aggcaagtac acttccagcc ttgaaaaagg gctttaaatt cagcatgcca tttatacagg
     5821 caggagtaaa cccatgacaa agttatacgc agaaatcgcc aagatggaga cgcaggacga
     5881 cgacacggtc aaggtttggg gttacgcttc aagcgaggaa atcgattcgg acggcgaagt
     5941 catcgcggcg gcagctatga aggcggcgat tcccgattat atgaagtttg gcgcggggcg
     6001 cgagatgcac ggctcaaacg ctgcgggaac ggcaattgaa atcaacgttg aagacgacgg
     6061 cataaccttt ttcggtgctc atatcatcga ccccgttgtc gtgtcgaaag tcaaaacagg
     6121 cgtttacaaa ggcttttcca tcggcggcag cgttaccgcc cgcgatgatt tgaacaagtc
     6181 gcaaatcacg ggcttgaagc tgacggaaat cagccttgtc gagcgccctg ccaatcccga
     6241 tgcggtgtct acctgcttta aggcggacaa aggtgcggaa acggtaaaca acgatacaga
     6301 acataatgct acatatttta gccatttccc ttccaaacaa aaaagcaccg acggcggccg
     6361 atgccccttc ctttacaggt tcccctattt tttatccgcg ggcagagccg gttttgc
//
LOCUS       DAWEWP010000111         6271 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWEWP010000111 DAWEWP010000000
VERSION     DAWEWP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.111"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..151)
                     /locus_tag="WGN53_001550"
     CDS             complement(<1..151)
                     /locus_tag="WGN53_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ0140527.1"
                     /translation="MLKTLTFAALHFSTAFSITYVLTGSIGVSGAVALVEPLINTVVF
                     YSGLTK"
     gene            273..1226
                     /locus_tag="WGN53_001551"
     CDS             273..1226
                     /locus_tag="WGN53_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0140528.1"
                     /translation="MNQKKVVVLDADTLPGRTFHFDFPHELAVYGTTDAAETAGRVRD
                     AHIIITNKVMISADIIAHAPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPAVREGYVAFENAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTNEPPKNGNLLLKARL
                     PNLIVTPHTAWASREALDKLFDILLANIHAFVKGEAQNQVV"
     gene            complement(1300..3354)
                     /gene="metG"
                     /locus_tag="WGN53_001552"
     CDS             complement(1300..3354)
                     /gene="metG"
                     /locus_tag="WGN53_001552"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ0140529.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKAGGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGVKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CGRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGKLKDVSDSALLAKLTAQSEAIAECYENREYAKALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTILTAYLAPVLPQTAAN
                     AARFLNLDAITWANTRVTLGEHTINKYEHLMQRVEQKQVDDLIEANKQSIQTTPAPVE
                     DSKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     SYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATAEGKLKLLDVDTGAQPGDK
                     VG"
     gene            complement(3470..5308)
                     /gene="glmS"
                     /locus_tag="WGN53_001553"
     CDS             complement(3470..5308)
                     /gene="glmS"
                     /locus_tag="WGN53_001553"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ0140530.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGSIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKSGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPTDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKNSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTVPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            5587..6105
                     /locus_tag="WGN53_001554"
                     /pseudo
     CDS             5587..6105
                     /locus_tag="WGN53_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247907.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1337 a         1484 c         1905 g         1545 t
ORIGIN      
        1 tttttgttaa tccactatag aaaacgacgg tgttgattag aggctcgacc agcgcgaccg
       61 cccccgatac gcctatactg cccgtcagta cataggttat actgaaggcg gtgctgaaat
      121 gcagtgcggc aaaagtcagg gttttaagca tcatcatctc tcggattgga cattgacgga
      181 gggatgataa agattatcat aaggctgtgc ggtttaaatt tgctatttgc tgttagtgta
      241 aataaatcat tttctaaata gggataaaaa ttatgaatca gaagaaagta gtcgtattgg
      301 atgcggatac tttacccggt cggacttttc acttcgattt cccacacgag cttgcggttt
      361 acggtacgac agatgctgcg gaaacggcag gacgggtgcg cgacgcgcat attatcatca
      421 ccaataaagt gatgatttca gcggatatca ttgcacacgc tccgcaactt gaattgattg
      481 ccgtcagtgc gaccggtgtg aacaatgtcg atattggggc ggcgaaggcg gcgggcgttg
      541 cggtatgcaa tgtccgcgcc tacggaaacg aatcggttgc ggaacacgcc tttatgctga
      601 tgattgcgtt gatgcggaat ttgcctgcct atcagcgtga tgttgcggca ggattatggg
      661 agaagtcgcc gtttttctgc cattacggcg cgccgattcg ggatttgaac ggtaaaacgc
      721 tggcggtttt cggacgtggc aatatcggac ggacgcttgc cggatacgcg caggcattcg
      781 gtatgggggt ggtgtttgcc gaacacaaac acgcgcccgc tgtgcgcgaa ggctatgttg
      841 cctttgaaaa tgcggtacgg gctgccgatg tgttgtcgct gcactgtccg ctgaacgcac
      901 aaactgaaaa tatgataggc gaaaatgagt tgcggcagat gaagcccggt gcggttttga
      961 ttaattgcgg gcgcggcggg ctggtggatg aaaacgcgct gcttgccgca ctcaaatacg
     1021 ggcagatcgg cggggcgggc gtggatgttt tgacgaatga gccgcctaaa aacggcaatc
     1081 tcctgttgaa ggcccgcctt cccaatctga ttgttacgcc gcataccgcg tgggcaagcc
     1141 gcgaggcttt ggacaagctg ttcgatatat tgttggcgaa cattcacgcc tttgtgaaag
     1201 gcgaggcgca aaaccaggtg gtttgagctt gtcgggatga cgaaaaaatg ccgtctgaac
     1261 gcccaaaggg ttcagacggc attgttttga tatttctgct taaccgactt tgtcgcccgg
     1321 ttgcgcgcct gtatccacat ccaagagctt cagttttccc tctgccgtgg cggcactcaa
     1381 aatcatacct tcggatacgc cgaattttgc cattttgcgc ggggcgaagt tggcgacggc
     1441 gatgaccatt cttccgttta actcggcagg gttgggataa gacgcggcga tgccggagaa
     1501 gatgatacgt ttttcaaaac cgaaatcgag gtcgaatttc aggagtttgg tgctgccttc
     1561 gacggcttcg cagttcaata ctttggcgac gcgcatatcg attttcataa agtcgtcgaa
     1621 acttgcctgt tcggcgactt tttcgtattt gctgtcttca acaggcgcgg gggttgtctg
     1681 aatactttgt ttgttggctt cgattaaatc gtccacttgt ttttgctcca ctcgttgcat
     1741 taaatgttcg tatttgttga tggtgtgttc gcccaaggtt acccttgtgt tcgcccaagt
     1801 aatcgcgtcc agattgagga aacgcgcggc gttggcggcg gtttgcggca acacgggggc
     1861 gaggtaggcg gtcagtatgg tgaaggcgtt gatgagctcg ctgcatactt cgtgcaggcg
     1921 ttcgtcttga ccttcttgtt tggcgagttc ccacggtttg ttggcatcga cgtattcgtt
     1981 cacggcatcc gccaaagcca taatgtcgcg cagggctttg gcgtattcgc ggttttcgta
     2041 gcattcggcg atggcttcgc tttgtgcggt cagttttgcc agcaatgcgc tgtcggaaac
     2101 gtctttgagt tttccttcaa agcgtttggc gatgaaacct gaagcgcggg cggcgatgtt
     2161 gacgtatttg ccgacgaggt cgctgttgac gcgcgagata aagtcttgca ggttcaaatc
     2221 gatgtcttcg attttgctgt tgagtttggc ggcgatgtag tagcgcatcc actcggggtt
     2281 taggccttgt tccaaatagg atttggcggt gataaacgtg ccgcgcgatt tggacatttt
     2341 ttgtccgtcc acggtcaaaa agccgtgtgc gtacacgccg gtcggggcgc ggtggccgga
     2401 gaaatgcagc atagcgggcc agaacagggc gtggaaatag aggatgtctt tgccgatgaa
     2461 gtggtacatc tcggtttggc tgccggcttt gaagtattcg tcgaaatcga tgccgatgcg
     2521 gccgcacagg tttttaaacg atgccatgta gccgacgggc gcgtccagcc agacgtagaa
     2581 gtatttgccc ggcgcgtcgg ggatttcaaa accgaaataa ggcgcgtcac gggaaatgtc
     2641 ccagtcggac agcgtagttt cttcgccttc gcccagccat tctttcattt tgttgagggc
     2701 ttcggcttgc agatggggct tgccgtcgtg cgggttgttg ccggaagtcc atgctttgag
     2761 gaagtcggcg cattcgccca gtttgaagaa gaagtgttcg gattcgcgca attcgggttt
     2821 cacgccggag acggcggaat acgggttgat cagttcggtc ggggagtagg tcgtgccgca
     2881 gacttcgcag ttgtcgccgt attggtcttg ggcgtggcat ttggggcatt cgcctttgac
     2941 gaagcggtcg ggcaggaaca tttgcttttc aggatcgaaa agctgctcga tgacgcggct
     3001 ttcgattttg ccgccggctt tcaatgcgcg gtaaatgtct tgggaaaact gtttgttttc
     3061 aggggaatgg gtgctgtaat aattgtcgta gccgatgaaa aagccggtaa agtcggcgag
     3121 gtgttcttcg cgcactttgg caatcatgtc ttcgggcgcg atgccttgtt tttgcgcggc
     3181 aagcatcacg ggcgtgccgt gggtgtcgtc ggcgcagcag tagtggcact cgtggccgcg
     3241 cagtttttga aagcgcaccc aaacgtcggt ttggatgtgt tcgaccatgt ggccgaggtg
     3301 gatgctgccg ttggcatagg gcagggcgga ggtaactaag attttgcgtg tcatattgtg
     3361 ctttgcaaac aatgggtaaa ggcggattat accgcaaatc aaacggggaa atgccgtctg
     3421 aagcctgaaa aatcgggctt cagacggcat ttttgccaac cggcggtatt tattcgacgg
     3481 ttacggattt cgccaggttg cgcggcttgt ccacatccgt gccgcgcgcg agggcggtgt
     3541 ggtaggcgag gagctgcacg gggacggtat gcacgacggg ggataattct ccgacatggc
     3601 gcggtgcgcg gatgacgtgc acgccttcgg tggcattaaa attgctgtcg aggtcggcaa
     3661 agacgaaaag ttcgccgccg cgcgcgccga cttcctgcat attggctttg actttgtcca
     3721 gcaggctgtc gttgggcgcg atgacgacga cgggcatatt ttcgtccacc agggcaagcg
     3781 gcccgtgctt cagttcgccg gcaggatagg cttcggcgtg gatgtaggtg atttccttca
     3841 gcttcaacgc accctcgagg gcaatcgggt aatggatgcc gcgccctaaa aacagcgcgc
     3901 tgtttttctt agcaaactgt tgcgcccaag cggcaatttg cggttcgagg ttcaaagcgt
     3961 gctgcacgct gccgggaagc tggcggagtt cttcggtgta acgtgcttca tcttcgccgg
     4021 ataccaaacc gcgcactttc gccagcgtta ccgccaaacc gaacagcgcg accagctgcg
     4081 tggtaaacgc cttagtcgag gcgacaccga tttccgctcc ggcgcgggta taaagcacga
     4141 ggctgctttc gcgcggcagg gcggattcca tcacgttgca gatggagagg ctgtggcggt
     4201 gtcccaagga tttggcgtat ttcaaagcct ccatcgtgtc cagcgtttcg ccggattggg
     4261 aaatggtaat gaccagttgg tccggatcgg caatcacgct gcggtatcgg tattcgctgg
     4321 cgatttcgac gtcggtcgga attttggcga tggattccaa ccaatatttg gcggtcagcg
     4381 cggcgtaata ggaagtgccg caggcaagga ttttgacgct gcggatgctt tcaaacacgc
     4441 ttttggcgtt tttgccgaag ttttcgggga tgaagccgcc gtcgaggaaa acctccgccg
     4501 tgtctgcaat cgcgcgcggc tgctcgtgga tttccttttg cataaagtgg ctgtacggcc
     4561 ccagctccaa agaggcgagc gagagttcgg ataccttgac tttgcgttcg gcaggcaggc
     4621 cgcttttatc agtcagcctt ttgatgccgt ctgaagccag cagtgcgatg tcgccgtctt
     4681 cgaggtacgc cacgcggcgc gtaaaggcga tgacggcgga tacgtccgaa gcgataaagg
     4741 tttcatcgtc gcccaaagcg accaaaagcg ggcagcccat acgcgccaca actaattcat
     4801 caggcttgtc ttgggcaata accgcgatgg cgtatgcgcc gtggaaacgt ttgaccgctt
     4861 cctgcacggc ttcaaacagc ctgccgccgt tttgcgcgta ttcgtgattg atgctgtggg
     4921 cgatgacttc ggtatccgtt tgcgattcaa aacggtatcc caaaccttcc aaacgtttgc
     4981 gttcgctttc aaagttttcg atgatgccgt tgtggacgac ggcaatcata ccgccgctga
     5041 tgtgcgggtg ggcgttcggc tcggtaacgc cgccgtgtgt cgcccagcgc gtatgcccga
     5101 tgccgatgct gccgctgatg cctttttcgc gtgctgcgtc ctccataagc tgcacgcgtc
     5161 cgacgcggcg cacgcgtttg attttgccgt cggtgttgac ggcaatacct gatgagtcat
     5221 acccccggta ttcgaggcgt ttgaggccgt cggtcagaaa atcgacgacg ttgtgatggg
     5281 cgcggatggc gccgacgata ccgcacataa ctgttcctta gtatccggtt gaaaaaaaac
     5341 agatgcggac ggctcccgtg ccgcaccttc cgcttcggat tataaaccgc ctagtgtcgg
     5401 aaaacagcaa aatgccgtct gaaggcttgg gcttgctcaa aaaaggaggg atttccccgc
     5461 ctgaccggga tgggcgttca gacggcattg cctgctgctg gtttatagtt tttgcaaatc
     5521 aatattgaca agctgaaaaa aaaaaacaat atactcgccc agtcttaatg ttaaaggagt
     5581 atggaaatga aacaaatgct tttggccgtc ggcgtggcgg cggtgttggc gggctgcggc
     5641 aaggatgccg gcggttacga ggggtattgg cgcgaaaagt cggacaaaaa agagggcgtg
     5701 attgctgtca aaaaagaaaa aggcaattac ttccttaata aaatcaacgt atttacaggc
     5761 aaggaagagt ctttgctttt gtctgaaaaa gacggcgcgc tttcgataaa cacggggata
     5821 ggggaaatcc cgatcaaact ttccgacgac gggaaagagc tgtatgtcga acgcaggcag
     5881 tatgtcaaaa ccgatgcggc gatgaaggac aaaatcatcg cccactagaa aaagtgcgga
     5941 caaacggaac aggcataccg cgacgcgcga aatgcgttgc cgtcaaacca aacgtatcag
     6001 cagcgtcagg cggcgatcga gcagttgaaa cggcggtttg aagccgagtt tgacgaattg
     6061 gaaaaagaaa tcaaatgcaa cggcaaaccg acattgttgc tttagcagag gacaaccggg
     6121 aggatgccgc cgtccgaatc ggacgtgcgg ttttcgtacc ggtacgggcg ggcaggaatg
     6181 tccgcttttt ttgttcggac gcgtttgaat acccgtttga ttccgtccgt ttgcaagggg
     6241 tatttccgtt cgggcggaaa ttatagtgga t
//
LOCUS       DAWEWP010000112         6255 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWEWP010000112 DAWEWP010000000
VERSION     DAWEWP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.112"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(391..825)
                     /locus_tag="WGN53_001555"
     CDS             complement(391..825)
                     /locus_tag="WGN53_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ0140531.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGN53_001556"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGN53_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ0140532.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2392)
                     /locus_tag="WGN53_001557"
     CDS             complement(1556..2392)
                     /locus_tag="WGN53_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ0140533.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     ETGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMENDE
                     QGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVKFE
                     LN"
     gene            2647..3285
                     /locus_tag="WGN53_001558"
     CDS             2647..3285
                     /locus_tag="WGN53_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ0140534.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3737..4537)
                     /locus_tag="WGN53_001559"
     CDS             complement(3737..4537)
                     /locus_tag="WGN53_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ0140535.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(5516..6157)
                     /locus_tag="WGN53_001560"
     CDS             complement(5516..6157)
                     /locus_tag="WGN53_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ0140536.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMMMSEKSGVPLYFDRAL"
BASE COUNT         1676 a         1565 c         1288 g         1726 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgtctgcg cagaaatgga gacggcgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacagcccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aatactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgagaaaa accactgctt ttcacgattt
     1681 taacggactc gacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 cgtcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttaccgct gccttctccg cgccccgttc cgtctccttt ggtaccggtt tcttccccat
     1921 taccctgctc gctgtctgct ttggcagaag cattgctggg atgttcggca ggtttttcag
     1981 acggcttctc gaccggtttt tccgccggtt tcgggacagg cttcgcttcc ggcttaggct
     2041 ctggtttcgg tttttcttcg ggtttcggct tttcttcagg tttcggctct tccttaggct
     2101 gctgaatatc cgcatccgcc tttttcgtaa ccaccggctt caaaaccggc ttgggcggct
     2161 cgacaggttt gggcggctca ggctcaggtt gcggctcagg cgcagcaggc gcacctgcgc
     2221 cttcgggtgc accgtcccct ccgccaaaat cacccaaatc gacaaattcg ataacatttc
     2281 ctgactctat cacgggcagc ttgtgcgcct gccagagcaa tgccaccatt gccaaatgca
     2341 gcagtgcgac ggaaaacacg actgcggggg ttaaaattcg ttctttatcc ataattcggg
     2401 cataataata gcaacaattc ctatttgcaa cctattttta caatttttgg tcatatgaat
     2461 gtctgttccg ttcacaggca aaccgtgttt aaacgctgta ttacagcaaa tcatcagata
     2521 acgggccggc agaaaaaatg attccgtctg atttcttatt ccaataaaat caggttagat
     2581 gatatattgc cgcttctgtc tgtcagccgt ttcgggctgc acaccacatc tgttcaaagg
     2641 aaaaccatgt ttcaaaattt tgatttgggc gtgtttctgc ttgccgtcct gcccgtgctg
     2701 ctcgccatta ccgtcaggga ggtggcgcgc ggctatacgg cgcgctactg gggagacaac
     2761 actgccgaac aatacggcag gctgacactg aaccccctgc cccatatcga tttggtcggc
     2821 acaatcatcg taccgctgct tactttgatg ttcacgccct tcctgttcgg ctgggcgcgt
     2881 ccgattccta tcgattcgcg caacttccgc aacccgcgcc ttgcctggcg ttgcgttgcc
     2941 gcgtccggcc cgctgtcgaa tctggcgatg gctgttctgt ggggcgtggt tttggtgctg
     3001 actccgtatg tcggcggggc gtatcagatg ccgttggctc aaatggcaaa ctacggtatt
     3061 ctgatcaatg cgattctgtt cgcgctcaac atcatcccca tcctgccttg ggacggcggc
     3121 attttcatcg acaccttcct gtcggcgaaa tattcgcaag cgttccgcaa aatcgaacct
     3181 tatgggacgt ggattatcct gctgcttatg ctgaccgggg ttttgggtgc gtttattgca
     3241 ccgattgtgc ggctggtgat tgcgtttgtg cagatgttcg tctgactggc tttcagacgg
     3301 cataaacgct ccagaaaacg cggcaggaca tattgccctg ccgcgttttc ctgtagtgta
     3361 atcttatttt tttcatcatt attagaacca agttgcatga taataccttt cattaactga
     3421 aacactgatt aagaaactcc agtctgtcta atgatgaggt tttcacatcg ccaaaacttg
     3481 ccaatcaaat gctggattta ttgccgtctg agatttggtc aaatccaaag gcgacattct
     3541 tagaccctgt gtgtaaatca ggggtatttt tgcgtgaaat cgtcaaacgc ttggatgaag
     3601 gcttgaccaa tcaaatacca gataaacaaa ctcgcattaa ccacatttta aaaaatcaag
     3661 tttttggaag tactgccacg tatgtaggta gctttgaccg atatttgcat aaaaactcct
     3721 ttgctggtga aaggaattat tttgccaatt ttaaaatatt tctagcacca aatagtacaa
     3781 tgacaaagac aatcatgcca atgattaaat caggatagct agaatgagtc aataacgtca
     3841 atgctcccgc cgctatcaca ccgatattga tgataatgtc attggatgta aaaatcatgc
     3901 tggctttgat atggatttct ttattttgat ttttgctcag tagatataag cacagccagt
     3961 ttgcaatcaa tgccaaaaat gccgtgccaa tcatcagttg ataattgggc agctgctcag
     4021 caccgataaa acgcctaatc acttctatca ccccaaataa cgccaatatt atctgcgtta
     4081 tccccgccaa aaatgccaca cgttttttat acgccagcgt cataccaatg gctgatagcg
     4141 ccaatatata gacaaagctg tccgccagca tatctagact atcagcaatc agccccatag
     4201 aattagcaaa aataccaacc gaacactcta tgataaaaaa cacaaagtta atcatgagca
     4261 cttgatataa taatcttttt tctaagtgct catcaggctt gttaaacact atcttatcaa
     4321 caatcacttc ggtggaaatg atatgactat caaaattaag cggttcaagt acttgtaaaa
     4381 tcgttgtatc ttgattatcg tgatagacgg ttaagcaccg cccagcaata tcaaactgta
     4441 attcataaat atcagacaca tcttttaaac gcatgcgaat gagctgttct tcggacgggc
     4501 agtccatttt ggtaatgtta aaaatggtct ttttcatcta tttagttcct tgttttgatc
     4561 aggttggctc aaataaatct gtgtttatat tgctgcttgg taatttttgg atggtttgag
     4621 taaattgatt aggttaaaat ttacctttgg aagtaccgcc acgcataata gtttagatat
     4681 gtttataatc tctggataaa aaaacgtaat aagtgtttgc tggataacaa agtccaaacc
     4741 aatagcagat aaaataaggc atccaccccc tttcttcatt aaggataaga gatatcgaga
     4801 aacaaatcat aactaaacag aaaaaatttt ggagataaag ccatttcatt cccctattca
     4861 agaatctggc caagataggt attttgtata ctctagaaaa gaaaggcatt tccaagggaa
     4921 acatatcaga taaaaacttt tgtttatttt ttactataga tagaacccta tttctcaaga
     4981 taaatccaaa tgcaatgcca attatcatta ttaataaaca aaggatagta taactatgga
     5041 taaccttcat taaacaaaaa gaagaaatgg cagataaatt acatacgata tagtggaata
     5101 taaaatattt acggtctaaa ccttgttcag ttgcaatttt tttatactta ccttgcgcaa
     5161 aaaaatcaaa gaaatccatt aaactatctt tcacattaga aatttaaaag ctaaataata
     5221 caacaaacaa tgtgaagtac tattcatggt ttatttaaaa aataatacta ttctgaacat
     5281 tatttagata cagaaattaa caaattagaa ctaaacaagc ttttaaatac tttaatttta
     5341 ttggaaagct ataaaaggaa ctataacttt acacactagt cacttctttt taagaggcaa
     5401 aagggattgg gaaggtcgtc ttggagataa gcactggtat ttcggccaat ggtaaataga
     5461 gtttacctca aatagggtag aacctccttc atctgtcagt taataacagc cacttttaca
     5521 atgccctgtc aaaataaagc ggcacgcccg atttttcact catcatcatc aaataaccca
     5581 tcaccttttg aggccattcg ataccgcgca ccacggtcag attcctcaaa acggggaaaa
     5641 ccaaaatatc ctccatgccg attccgccgt tgatgccgtc tgaagcaccg tccatcaaat
     5701 tctccaactc ttgcaaatct gcgtttatgc gttcgagata ttgggcggtt ttattcaaat
     5761 tggcggaaaa gctgccgatg cttttctctt ttttgtctgt aaaatatttt accgcttccg
     5821 gcgttgcaaa ttcgggcagc ccgattttga tcacgcgcgg ctgcaccagt ttgtcgttgt
     5881 atccgcccac cttgtccagc cacgcccgta tctcggggcg gacttcgtct ttcagacggt
     5941 cttcgcggtc gaaatgccgc acaatgtcca aactctcgcc cataaacgaa ccgtcttctt
     6001 tttgcaggac gggcacttgt ttcgcaccga tcataccgat cggcgttgcc tcgtcgtcgt
     6061 ttgccagcac ggcttcttca acgtccgcgc caaacagccc ggcagccatc cgcgcacgca
     6121 cacaaaacgg gcaatggtcg taaatataca gtttcatcaa aatattcctc gtcaacctgt
     6181 cggtaccggc taccttaaca ccccgcgcca cccgaaacaa gtttatcttc ccgcctatgc
     6241 accgtaaata aataa
//
LOCUS       DAWEWP010000113         6244 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWEWP010000113 DAWEWP010000000
VERSION     DAWEWP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.113"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(550..1134)
                     /gene="rnhB"
                     /locus_tag="WGN53_001561"
     CDS             complement(550..1134)
                     /gene="rnhB"
                     /locus_tag="WGN53_001561"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ0140537.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAAGPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLFAQQALF"
     gene            complement(1202..3097)
                     /gene="mnmG"
                     /locus_tag="WGN53_001562"
     CDS             complement(1202..3097)
                     /gene="mnmG"
                     /locus_tag="WGN53_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ0140538.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKATIREMLENQENLDLFQQAVEDVTLDGERISGVVTAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGSADMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIQGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3372..4799)
                     /locus_tag="WGN53_001563"
     CDS             complement(3372..4799)
                     /locus_tag="WGN53_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0140539.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            4959..5948
                     /gene="pdxA"
                     /locus_tag="WGN53_001564"
     CDS             4959..5948
                     /gene="pdxA"
                     /locus_tag="WGN53_001564"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ0140540.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFCIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
BASE COUNT         1292 a         1637 c         1827 g         1488 t
ORIGIN      
        1 tcattcccgt aaaaacaaaa aatcaaaaca gaaaactgaa atatcgtcat tcccgcgcgg
       61 gcgggaatct aggtctgtcg gtacggaaac ttatcgggaa aaacggtttt tccaaccctg
      121 agactccgga ttcctgtttt cgcgggaatc cggttttttg agtttcagtc atttttgata
      181 aattcttgta gctttgagtt tctagattcc cgcttttgcg ggaatgacgg tttggaagtt
      241 acctgaaatt caaaaaaaaa cggaaaccgg acggattgga ttcccgtttt cacgggaatg
      301 acggatttta ggtttctttt ttgattttct gtttttcgcg ggaatggcgg tttggaagtt
      361 acctgaaatt caaaaaaaaa aaacggaaac cggacggatt ggattcccgc ctgcgcggga
      421 atgacggatt ttaggttttt ttttttgatt ttctattttt cgcaggaatg acggtttggg
      481 ttctttctct ttggagttgc gatgccggaa atgccgtctg aaggcttcag acggcatttt
      541 tgtgccggtt taaaacaagg cctgctgcgc gaacaggttt ctgacggggg cgaagtcgcg
      601 gcggtgttcg ggcagcacgc cgtatttttc gagggcttcc aaatgctgct tcgtgccgta
      661 acctttgtgt ttgtcgaaac cgtattgggg atggcgttgc gccagtgcgt acatttccgc
      721 atcgcgggcg gtctttgcca aaacggatgc ggcggatatt tcgatgattt tgctgtcgcc
      781 tttgacgacg gcttcggcag ggatgttcaa atgttcggga atgcggttgc cgtcgatgaa
      841 tattttttcg ggacgcacag ccaaaccgtc aacggcgcgt ttcatcgcga gcatggtggc
      901 gtgcaggatg ttgaggctgg cgatttcttc gggcccggcg gcggcgacgt gccacgcaac
      961 cgcctgattt tttatcattt cggcaagcgc gtcgcgtttt ttctcgctga gttttttgga
     1021 gtcggtcagt ccgggcaggt cgaatatttc cggaaggatg acggcggcgg caaacacgct
     1081 gccgactaaa ggcccgcgcc ctgcctcgtc cacgccggcg gtcagtatgt gcatgatgtt
     1141 tcctgtcggg atggcacgat gccgtctgaa cggtgtttca gacggcatcg cgccgatgtg
     1201 tttatttcgc gtctttaaac ccgcgcttca aatgcaccat cagcaatgcc actgccgcag
     1261 gcgttacgcc ggaaatgcgg cttgcctgcc cgacggtttc aggtttgtgc tggttgagct
     1321 tttgctgcac ttctgccgac aagcctttga ctttgccgta atcgatgccg tcgggcagtt
     1381 ttaaggtttc gatgtcgcgg cggctgtcga tttcttcgtt ttggcggtcg atatagcctt
     1441 ggtatttgac ttggatttcg acttgttcga ccacttcggc ggagaggcgt tcagacggca
     1501 tcgcgccttc gagcgtcatc agcgcggcgt agtcgaggtt cgggcggcgc aggaggtcgt
     1561 gcaggttggc ttcgcggctg agcttttgcc cgaatacgcg gatttgttcg tcttcggcga
     1621 gtttttgcgg cgtgtaccac gttgttttca aacgttggat ttcgcgttcg atggcttcgc
     1681 gtttttcgtt gaacatgcgc cattgcgctt cacccaccaa gccgattttg tagccgtctt
     1741 cggtcaggcg catatcggcg ttgtcttccc tgagttgcag gcggtattcg gcacggctgg
     1801 tgaacattcg gtagggttcg ttcacgcctt tggtgatgag gtcgtccacc aatacgccga
     1861 gataggcttg ttcgcggcgc agcaggagcg ggtcttgttc gcggacgtat tgcacggcgt
     1921 tcgcgcctgc cagcagtcct tgcgcggcgg cttcttcgta gcccgttgtg ccgttgattt
     1981 gtccggcgaa gaagaggcct tggatggttt tggtttcgag gctggctttg aggttgcgcg
     2041 ggtcgaagta gtcgtattcg atggcgtagc cagggcgcag gatatgggcg ttttcaaggc
     2101 ctttcatgct gcgcacgagc gcgatttgga tgtcgaacgg caggctagtg gagataccgt
     2161 ttggatagta ttcgtgcgtg gtcaggcctt cgggctcgag gaaaatctgg tggctgtctt
     2221 tgtcggcgaa gcggttgatt ttgtcttcga tagacggaca ataacgcgga cccacacctt
     2281 cgattttgcc agtaaacatg gggctgcggt cgaagcctga acggatgatg tcgtgggttt
     2341 gcgtgttggt atgcgtaatc cagcaggaca cttggcgagg gtgcatatcg gcgctgccgc
     2401 gtacggacat tacgggaacg ggcgtgtcgc cgggctgttc ggtcagttgg gagaagtcaa
     2461 tcgtgcgccc gtcaatacgc ggcggcgtgc cggttttcag acggccttgc ggcaggttca
     2521 attcgcgcaa acggccgccc agcgatttgg cggcggggtc gccagcgcgg ccgccttcgt
     2581 agttttccaa accgatgtgg attttgccgg acaaaaacgt gccagcagtt agcacgacgg
     2641 cgcgtgcttt aaactccacg cccatcgcgg taaccacgcc gctgatgcgt tcgccgtcga
     2701 gcgttacgtc ttcgacggct tgttggaaaa ggtcgaggtt ttcttggttt tccaacattt
     2761 cgcggatggt ggctttgtac aggatacggt cggcctgcgc gcgcgtggct cgcactgccg
     2821 cgcctttgct ggcgtttagg cggcggaact ggatgcctga tttgtcggtg gccaacgcca
     2881 tcgcgccgcc gagcgcgtcg agttcgcgta ccaaatgccc tttgccgatg ccgccgatag
     2941 aggggttgca cgacatttgt ccgagcgttt cgatattgtg tgtgagcaaa agcgtctgcg
     3001 cgcccatacg ggcggcggcg agcgcggctt ccgtgcctgc gtgtccgccg ccgacgacga
     3061 taacgtcgta ggttttgggg taaatcatgt gggtcatgtg tgtattgcct gacggtgttt
     3121 cagacggcat ttatagtgga ttaacaaaaa ccagtacagc gttgcctcgc cttgtcgtac
     3181 tgcttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttgaacc actatattca
     3241 atatgccgtc tgaaaaacga aatggattca aaagtaaagg gttgggattg tacgcttgtt
     3301 cgccctgttt ttacagtgtg cggaaaggga aaagccgctt cgcggggaag cggctccggt
     3361 aagggcggga tttaccaaac gtcggatttg atacggcgtt tcaggcccgg atgttcggaa
     3421 agtttgaact cggggtcttt gcccattttc agcttggcgg tgtaatcgcg cagcagcata
     3481 aacgccaagg gcgagagcag caggatggcg acaaggttga tccacgccat aatgcccatc
     3541 gccatatccg ccatatccca gaccaaaggc acattggcaa ccgcgccgaa atagacccac
     3601 gccaaaacca gcatacggaa aacggcggta atcagccaat ggcttttgat gaattggacg
     3661 ttggactcgg cataggcata gttgccgata acggtggaaa aggcaaacat aaacaggatg
     3721 acggcgagga agcccgcgcc ccattgcccc acttggctga caatcgccgc ctgcgtcagc
     3781 gccgcaccgc tcaaatcgcc gtaaggctgt tggtaaatca agatgatgaa ggcggtgcaa
     3841 gaacaaacga tgatggtatc gacaaacacg cccagcattt gaatcatacc ttgcgaaaca
     3901 gggtgtttca cttcggcggc ggcggcggcg ttcggcgcgg aacccatacc cgcctcgttg
     3961 gaatacaggc cgcgtttgat gcccatcatc atcgtttgcg aaatcagacc gccgagtaag
     4021 ccgcctgctg ccgcgtcgaa tttgaacgcg cccgaaaaaa tctgaccgaa cacgtccgga
     4081 atcatcggaa tattggtcaa aatgatgaaa agcgcgataa agaggtacaa aaccgccatc
     4141 agggggacga cgatttccgc cgctttagat atgcgcctga tgccgccgaa gataatcggc
     4201 gcggttaaaa tcaccagggc gacgccgaca taatgaggct cccaacccca cgccgctttg
     4261 acggtatcgg caatggtatt ggtctgaacc gcttcaaaca caaagccgaa acagaaaatc
     4321 aggctcaggg cgaacaacac gcccagccat ttctgcccca gcccttgagt gatgtagtag
     4381 gcagggccgc cccggaaatg gtggttgtcg tagtcgcgga ctttaaagag ctgcgccagc
     4441 gaagattcga caaacgccga actcataccg attaaggcgg ttacccacat ccaaaacacc
     4501 gcgcccggcc cgccgacttt gatggcgatt gccacgcccg cgatattgcc cacgcccacg
     4561 cggctggcaa ggccggttac aaatgcctga aacggcgtga tgccgtgagg gtcgtccccc
     4621 tgtttgcggc cgccgagcat ttctttgatg ctgcgcccga acaggcggaa ttggacaaag
     4681 cccgtggtta cggtgaagaa aagccccgta cccaaaagca tataaaccaa gtatgaccac
     4741 atcggatcgt tgatggcgcc gacccagccg tgcagccatt cggtaaagtt ctcgttcata
     4801 tcgcttcctt aaagttgaaa ctcgcacata ttggcggtat gcaagcaggg tttaaatttt
     4861 gtaaacgccc attctagcag attgtcaaca aaatcagaaa aatttacatc gccgcgcggc
     4921 tgcggcgtta gaatcgcatt ttgtttggag caaacacgat gaaacagcct gtttttgccg
     4981 ttacttccgg cgagcctgcc ggcatcggcc ccgatatttg tttggacttg gcgtttgcac
     5041 gcctgccctg ccgctgcgcg gtattgggcg acaaacacct gttgcgcgcg cgcgccgaag
     5101 ccttgggcaa aagcgtcgtc ctgcgcgact tcgatccaga atcaggcggc gcggcatacg
     5161 gcgagctgga agtgctgcac atccctgccg tcgaagcggt tgaggcgggc aaactcaatc
     5221 ccgccaacgc cgcctatgtg ctgcaacttt tggacaccgc gctcgcaggc atttcagacg
     5281 gcattttcga cggcatcgtt accgcgccgc tgcacaaagg catcatcaac gacgcgcgcg
     5341 caagcacagg ttttttcagc ggacacaccg aatatctggc ggaaaaaagc ggcacggggc
     5401 aggtcgtgat gatgcttgcc ggcaaaggcc tgcgcgtcgc cctcgtaacg acccacctgc
     5461 cgctgaaaga cgttgccgcc gccatcacgc aaccgctgat tgaaagcgtc gcacgcattt
     5521 tgcatcacga cttaaaacac aaattctgca tcaaaaatcc caaaatccta gtcgccggac
     5581 tcaaccccca cgccggtgag ggcggacacc tcggacacga agaaaccgac accattatcc
     5641 ccgcgctcga aaacctgcgc cgcgaaggga taaacctcgc cggcccgtat ccggcggata
     5701 ccttgttcca accgtttatg ctcgaaggtg cggatgccgt attggcgatg taccacgacc
     5761 aagggctgcc cgtgttgaaa taccacagtt tcgggcaggg cgtgaacatc acactggggc
     5821 tgccctttat ccgcacctcc gtcgatcacg gcaccgcgct cgatttggcg gcaaccggca
     5881 gggcggattc cggcagcctg ataactgccg tggagaccgc cgtcgagatg gcgcgcggca
     5941 gcctttaaag atgataaaag acccgtcatt tccgcgcagg cgggaatccg gtctgttcgg
     6001 tttcagttgt ttttgggttt cgggtaattt ccaaatcgtc attcccgcgc aggcgggaat
     6061 ccagaccttc gggcgacggc aatattcaaa gattatctga aagtttgagg ttctagattc
     6121 ccgttttcac gggaatgacg aaaggttgcg ggaatccggc ttgttcggtt tcggtttttt
     6181 ttgaggtttc gggcaacttc taaaccgtca ttcccgcgca ggcgggaatc caggcctttg
     6241 ggcg
//
LOCUS       DAWEWP010000114         6203 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWEWP010000114 DAWEWP010000000
VERSION     DAWEWP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.114"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            272..718
                     /gene="yciA"
                     /locus_tag="WGN53_001565"
     CDS             272..718
                     /gene="yciA"
                     /locus_tag="WGN53_001565"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ0140541.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIAQGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPILAGLLPTP"
     gene            complement(769..1590)
                     /locus_tag="WGN53_001566"
     CDS             complement(769..1590)
                     /locus_tag="WGN53_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0140542.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPNMVARGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1709..2167
                     /locus_tag="WGN53_001567"
     CDS             1709..2167
                     /locus_tag="WGN53_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0140543.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2332..3447)
                     /gene="lst"
                     /locus_tag="WGN53_001568"
     CDS             complement(2332..3447)
                     /gene="lst"
                     /locus_tag="WGN53_001568"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ0140544.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKTGDETGGTVRILLGSPDKEMKEISEKAAKDFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKN"
     gene            3548..3652
                     /locus_tag="WGN53_001569"
                     /pseudo
     CDS             3548..3652
                     /locus_tag="WGN53_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3650..3820)
                     /locus_tag="WGN53_001570"
     CDS             complement(3650..3820)
                     /locus_tag="WGN53_001570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140545.1"
                     /translation="MAIMAYFGGFVRALFDLGVFERGGVKGRGKWFSRVWRRSEVLCL
                     MCGGIFSVKLIL"
     gene            3880..6105
                     /locus_tag="WGN53_001571"
     CDS             3880..6105
                     /locus_tag="WGN53_001571"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ0140546.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTSDIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVIVPEATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAEGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNGKSPKRKAGELDNRGSHFYLTLYWAQELAEQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAVDIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1662 a         1814 c         1387 g         1340 t
ORIGIN      
        1 tgacgcggcg ggaatctatc ggaaatgact gaacccccga gattctagat tcccactttc
       61 gtgggaatga cggttcagtt gcttagggta ggatttggcg ggattggcgg actgaagccc
      121 actctacagc cccgccctac aatacgcctt gcgaatctgt cccacgcccg cccttgtatg
      181 tctcaaatgg gttaaactac gcacttcccg acttcccgtt tcgggcaggg gtgcaggttc
      241 gatttatctt tcaataaaac aaggaaactt tatgcagcac gaagaaggca accgccaacg
      301 ccctcaaggc gaactgctcc tgcgtaccgt cgctatgccg cgcgatacca atcccaacca
      361 agacattttc ggcggctgga ttatgtcgca gatggatttg ggcggcggca tattggcagc
      421 ggaaatcgca caaggacgca tcgttaccgt cgccgttcag gaaatgaact tcatccgccc
      481 ggtcaaggtc ggcaacgtcg tctgctgcta cgggcattgc gtccgcgtgg gcaatacttc
      541 cctccagctt aaagtcgaag tctgggtgaa aactttgatg aacgattgcg ttaccgaaga
      601 ccgccacctc gtaaccgaag ccgtgttcac ttatgtcgcc atcgatgcgg aaggcaatcc
      661 gcgcccgatt ccgaaagaag gcaaccccat attggcgggc ttattgccta ctccgtaaaa
      721 atgcccgtaa aaatgccgtc tgaaactgtc ttcagacggc attccgcatc atttgaacca
      781 agatctgatg cgggaaaaca gcgacatact ctgtttttca aagtcttccc gcacttccgg
      841 cacggctttt tttatcacct tcattcctgc aaaagtctgc gccacgggca taatttcaat
      901 cgtgttcaca ttcatatgcg ccggccggcg gtacagccac aatgcggttt ccgcaatatc
      961 ttcggggcgg ataaattcca caccctcata cacgcctgct gccttctcgt catcgccttt
     1021 gaagcgcacg ttggaaaact ccgtattgcc gcacaaaccc ggctcgatat tggtaacgcg
     1081 gatgttctta tccgccaact ccgcgcgcaa attcaggctg aactggcgca caaacgcctt
     1141 ggtcgccccg taaacgttgc tgccggcata agcataattg cctgcaatcg aacccaaatt
     1201 catcacataa ccgccgcccc gagccaccat attcggcaaa atcttgcgcg ttagaaacgt
     1261 caaacccaaa acattggttt gaatcattgt ttcccaatct tcaaaatccg ccttgtccgc
     1321 cgtgtccaga cccaaagcca gcccggcatt gttgatgagg cagtcgatgt cggaaaattc
     1381 atcagggatg ccgtttaagg cgttttccac cgactcgcgg cgcgacacgt ccatttccaa
     1441 agggtaaaac aaagcaccca attcatccgc caaggcctga agcctgtccg cacgacgcgc
     1501 cgcaccgata acgcggtatc ccgccccgac aaacgcgcgg cacatcgctt cgccgaatcc
     1561 tgccgaagca ccggtaatta aaacagccat tgtttttcct ttctttctcg tttcccaaac
     1621 ctgtttcagg tatcataata cccttcagac ggcatcgcgc cggctgaaaa tgacaacaac
     1681 cataccacca cgacaggaca cattcaatat gaaaacccaa atcagccttg ctgctgccgc
     1741 catcacactg ctcctttccg cctgcggagg cagcggaata ccttcccaac ccaaaggcga
     1801 aatttccgaa aaccgcaccg ccgcgttcaa atctatgatg cccgacttct cgcgtatggg
     1861 caaaatggtc aaaggcgaag aaccttacga tgtcgaaaaa ttcaaacagg cggcagcggc
     1921 gtttgccgac agcagcaaaa aaccgtttac gctttttgag tccgacccgc aaggcaacgg
     1981 gcgcgcactg cccgccgttt ggtcggatgg tgcaaaattt gaagccgaaa aaacaaaatt
     2041 cgccgccgcc gtcgaaaaac tcaacgccgc cgcccaaacc ggcaaactgg acgaaatcaa
     2101 agccgcctac ggcgaaaccg gcgcaagctg caaatcctgc cacgacagct tccgcgcgcc
     2161 cgaataacat ccaaaacaaa acaatgccgt ctgaagactt cagacggcat tttctttgcc
     2221 atttcagcac acgcaaagca atcagaaatt atcgtcaatc caaatgaaaa aacgctatcc
     2281 ttatttttac cccacgcact ccgccatttt ctattttgat ttgctatgcg attaattttt
     2341 atcgtcaaat gtcaaaatcg ggatgccgga ttgggtaaac agggcataca ccggcttgag
     2401 ccaataatct tctggaaggg aagccggttt caatgcgtaa acgtgcacat tgggaaaatc
     2461 cttcatcgtc aacgccgcgc cgctgaaaaa ggtataaatt tcatacctcg tatgcgggtt
     2521 tttcttaatt tcgcgcaaaa tatagtcttc gatgacatag ggcgaattta atgtggttac
     2581 gccggaaagc ccgtaggttt ggcgggggtg cggcgcgaca tattgtatgt tgaaatcttt
     2641 tgccgccttt tccgaaattt ccttcatctc tttgtcgggt gaacccaaaa gtatccgcac
     2701 cgtgccgccc gtttcgtccc ccgtcttcag ttcggacgca tcgaacagcg gcaggtaagt
     2761 catcttgcgg cggccgtcgt ccataatgtt tttcaaaccc ttgaatatcg tgtaatgctc
     2821 gtcggaagca ttgcgggttt tggcgatgct ccaatctccg atcatcatcc gggcaaaatt
     2881 ccgcttgatc gtcccgttta cagaaaactc atcgcccaaa tagctgctgc tttgaattaa
     2941 attgcctgtc ccgtcgtcaa aggttttgat ttccgcatcc gggtaagtgc tcaaaaaggc
     3001 ggcaatgctg actttttcca aacttgccaa ataaatccgc ttgactttcg gcagcagcat
     3061 cgactttacc ttcagctccg ccatcgtcgg aatgaaatta aacgatttgt tcaaaccgta
     3121 gggcaggtgg aaaaaatacg cccgctccgc cttatccttt atctgattga aataataatc
     3181 gtatttttca ttcctgtttt cagacatcag caccacataa aaccgctccc ccggatgctg
     3241 cgccataatc ctttccgcca ccttcatctg caatatggta tagcagaaaa tcagattgac
     3301 cggttccccc tcttcattga agagtttgtc cttcagcagg gaaaccgcat tcctttcccc
     3361 ctgatttacc cggtcaaatg tataaaatat cccgaaacaa aaaacaatca aacacaacac
     3421 ggtcaaacaa gcctttttca agcccatatc cctaaaactc cattccgaca aattgaacat
     3481 acgccccgag tgtcccggga cggaaaaatg cggaatttta gcagatattc cgacagttac
     3541 cgaatattat agtggattaa atttaaatca ggacaaggcg acgaagccgc agacagtaca
     3601 agcagtacga caaggcgagg caacgctgta ctggtttaaa tttaaaccac tataaaatca
     3661 atttcaccga aaatatgccg ccgcacatca ggcacagcac ctccgaccgc cgccaaacgc
     3721 gggaaaacca tttccccctt cctttcacgc cgccgcgttc aaacacgcca agatcgaaca
     3781 aagcgcgaac aaagccgcca aaatatgcca taatagccac gcacattccc cgcgcaatgt
     3841 gttccaaaag aaaaaaccaa cctacaagag aataatccta tgactcaaaa atccaccatt
     3901 gtttataccc ataccgacga agcacccgcg ctggcgaccc aatcgctgct gccgattgtg
     3961 caggcttttg cccgccacgc cgatattgat gtcaaaacca gcgacatttc tctctccggc
     4021 cgtattttgg cggcgtttcc cgaatacctg accgaagcgc aacgcgtacc tgatgcgctt
     4081 gccgaattgg gcgaactggt gaaacaaccc gatgcaaacg taatcaaact gccgaacatc
     4141 agcgcgtccg taccgcaact gactgccgcg attaaagaat tgcaatctaa aggctttgcc
     4201 gttcccgact atcccgccga cccgcaaacc gatgaagaaa aagccgtacg cgaacgctac
     4261 gaccgcatca aaggcagcgc ggtaaaccct gtcctgcgtg aaggcaactc cgaccgtcgc
     4321 gcccctaaag cagtgaaaaa ctttgcgaaa aaaaatccgc acagcatggg cgcatggacc
     4381 aaagactcca aaacccacgt tgccaccatg caaagcggcg acttttttca taacgaacaa
     4441 tctgttatcg tacctgaagc gacttccgta tccatcgtat tcaccgacaa acaaggcaac
     4501 aaaaaagagc tgcgcgagcc cgtcgccctg aaagccggcg aaatcatcga cgcgaccgtg
     4561 atgagtaaaa aagccctgct cgccttcctt gccgaacaag taaaagatgc gaaagcaaaa
     4621 ggcgtgttgt tctcgctgca catgaaagcc actatgatga aagtgtccga cccgattatc
     4681 ttcggacacg ccgtcaaagt attcttcgcg cctgtttttg aaaaattcgg cgacaaactg
     4741 gctgccgcag gtgtcaacgt caacaacggc ttcggcaacc tgcttgccaa tctggacaaa
     4801 ctggatgcgg acacccgcac cgccgtcgaa gccgaaatcg ccgccgttta cgctgccaac
     4861 cccgatttgg cgatggttga ttccgacaaa ggcatcacca acctgcacgt tcccagcgat
     4921 gtcatcgtcg atgcctctat gcctgcgatg attcgcaatt ccggccgtat gtgggacaaa
     4981 aacggcaaag cgcaagacac caaagccgtg attcccgaca gcagctatgc cggcgtttac
     5041 caagcaacca tcgacttctg ccgcgaacac ggcgcatttg acccgacaac catgggtact
     5101 gtgcccaacg tcggactgat ggcgcaagcg gcggaagaat acggctcgca caacaaaacc
     5161 tttgaaatcg aagccgacgg tcaggttcaa gtcattgatg cggaaggaaa agtcctgatg
     5221 caacacgacg ttgaagcagg cgacatctgg cgtatgtgcc aaaccaaaga cgctccggtc
     5281 aaagactggg tgcaactcgc cgtcaaccgc gcccgcctga gcaacacgcc tgccgtgttc
     5341 tggctcgatg aaaaccgtcc gcacgacaag agcctgctcg ccaaggttaa agcctacctt
     5401 gccgaactgg ataccaatgg cctcgacatc cgcgtcctcg ctcctgaaga agccgccaag
     5461 ttcagcttgg gtcgtctgaa aaacggcgaa gacaccatct ccgtaaccgg taatgtcttg
     5521 cgcgactacc tgaccgactt gttcccaatt ttggaactcg gcacaagcgc gaaaatgctg
     5581 tctatcgttc cattaatgaa cggcggcggt atgtttgaaa ccggcgcggg cggttctgca
     5641 ccgaaacacg ttcaacagtt cctcgaagaa aaccacctgc gttgggactc tttgggcgaa
     5701 ttcctcgcac tcgccgtatc gtttgaacat ttggcgcaaa aaaccggcaa tgccaaagcc
     5761 caagtcctcg ctgacacttt ggatgccgcc accgaaaaac tgctgttgaa cggcaaatcg
     5821 cctaaacgta aagccggcga actcgacaac cggggcagcc atttctacct taccctctac
     5881 tgggcgcaag aattggcgga gcaagacaaa gatgccgaac tgaaagccgc atttacgcca
     5941 ttggcagccg ctttgaccgc cgacgaagcg aaaatcgttg ccgagctttc cgccgtacaa
     6001 ggcaaagcgg tcgacatcgg cggctactac gcagccaatc ctgaaaaagc ggcacaagtg
     6061 atgcgtccga gcgtaacctt taatcaggca ctaaacgcct tataagcaca aaggtaaaaa
     6121 tgccgtctga acattcgtcg ttcagacggc attagttatc cctatctgcc tgattatgat
     6181 taaacgccgc acccattgga tat
//
LOCUS       DAWEWP010000115         6155 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWEWP010000115 DAWEWP010000000
VERSION     DAWEWP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.115"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..785)
                     /locus_tag="WGN53_001572"
     CDS             complement(<1..785)
                     /locus_tag="WGN53_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140547.1"
                     /translation="MNITIVAPYCSLPSEPHFNRFWYLAELLSQSHDVLLITSNFKHY
                     DKSFRRPEDAEAASQGRLKVMLLEESGYSKNVSLGRVTSHYRFVKHFEKWLENCRPGE
                     QDVVFSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHNLLPFA
                     SRANQAYRYADALVAVSQTYLDRAKEANPNVPGEVVYIGADFPKLDAAPAKNFGDDKI
                     RFFYLGTLSYSYDVETVCKGVRKLLDAGENVELHIMGGGPDLD"
     gene            complement(799..1871)
                     /locus_tag="WGN53_001573"
                     /pseudo
     CDS             complement(799..1871)
                     /locus_tag="WGN53_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            complement(1868..3283)
                     /locus_tag="WGN53_001574"
     CDS             complement(1868..3283)
                     /locus_tag="WGN53_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ0140548.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLPLTVG
                     LLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRHAPFSPAVLHRGLRYGIPIA
                     LSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRAI
                     EENAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPPLF
                     CTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFWLF
                     FVFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAVYLAGCI
                     LRHWKDLHKLFHYLKKQGFPL"
     gene            complement(3478..4587)
                     /gene="ribD"
                     /locus_tag="WGN53_001575"
     CDS             complement(3478..4587)
                     /gene="ribD"
                     /locus_tag="WGN53_001575"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ0140549.1"
                     /translation="MFSDTDISMMETALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETAGGK
                     IDLNHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNDNAF"
     gene            complement(4620..5084)
                     /gene="nrdR"
                     /locus_tag="WGN53_001576"
     CDS             complement(4620..5084)
                     /gene="nrdR"
                     /locus_tag="WGN53_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ0140550.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPSCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTKHRLYTSGQRD
                     IPSAALADMVLKELLEHNTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            complement(5113..5955)
                     /locus_tag="WGN53_001577"
     CDS             complement(5113..5955)
                     /locus_tag="WGN53_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ0140551.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT         1278 a         1558 c         2016 g         1303 t
ORIGIN      
        1 ctgtctaaat cggggccgcc gcccatgatg tgcaactcga cgttttcgcc ggcatccaac
       61 agcttgcgaa cgcctttgca cacggtttcc acgtcatagc tgtaactgag cgtgcccaag
      121 tagaaaaagc ggattttgtc gtcgccgaaa tttttggcag gcgcggcatc gagttttgga
      181 aagtcagcac cgatatagac gacttcgccg ggtacgttcg gattggcttc tttggcacgg
      241 tcgagataag tctgcgatac ggcgaccagc gcgtcggcgt agcggtaggc ttggttggcg
      301 cgtgaagcaa agggcagcag gttgtgtggt acttttttca aaaacggtac gactgaggag
      361 aaagactccg gccatacgtc ctgtacatcg acaatcagct tgtagcccaa acgcgctttg
      421 tgtttgccca acagcaggtt ggtggcaatc agcggatagg cggaaaagac gacgtcttgt
      481 tcgcccggac ggcagttttc caaccatttt tcaaaatgtt tgacgaagcg gtaatgactg
      541 gtcacgcgcc ctaaagacac gtttttgctg tatccgcttt cttccagcaa catcactttc
      601 agacggcctt gtgaggcggc ctcggcatct tcgggccgtc tgaaagattt gtcgtagtgc
      661 ttgaagttgc tggtaatcag caatacgtca tgcgactgcg acaacagctc ggccagatac
      721 cagaagcggt tgaaatgcgg ctcggacggc agcgagcaat aaggggcgac gatggtaatg
      781 ttcatgtttc agacggcctt acatttcgta cgcagccatg gtggtgcggt agatttcgtc
      841 gtcggagcat aaagtttcga cgtgtttgtg cagggctttg cccatttgac cgcgcagctc
      901 tggatgcttg atgagtgtat cgacggcttc gataaatgct tcttcatcgc cgaaaggaat
      961 gcagtagccg gtttggccgg tgatgaccat ttcggaaata cctgccatgt tgtaggtcac
     1021 gacgggtgtg tcgtaaaggc cggcttccaa aatattgttg ccgacgcctg cgccgtggtc
     1081 acctacgcaa tgaggcgtgt tcaccaaaat atcgacttct ttgaagtagc cggtcaagtc
     1141 gcggacgccg ccgaggaagg taactttgtc ttcgatgccc aaacgtttgg cttgggcttt
     1201 gaggttgtcc atttcttcgc cgatgcctgc aacgttgagg catacaggca taccgcggtc
     1261 aaccattttc ttcaaaatat ccagcattaa atgcacggcg cggacggtgt ccaaacggga
     1321 aagtgtgccg agtacggcgc attctttttt ggttttccgg aaatgaaatt ccgggggggg
     1381 gggagtgcgt tgtaggcgta ggcaatccgg ccggcgggga agccgtggcg gatgagtttt
     1441 tcgcgctcgt gtttacagtt gccgatgatg tacacgccca gcttgtcgaa gagcttggcg
     1501 attttggggt aggttgccgc gtccaaaccg tgcgagtggt agatgatttt ggttttcggc
     1561 gaaacgattt tggcggcaag ggcgcaggct ggaacaacgc gcgccatttg gcagtcgatg
     1621 atgtcgggtt gttcgcggcg cagcatccgg gcgtaggcaa aggtgctttt gaggtagccg
     1681 gcaagcccgc cccggtaaaa atcaaccggc tgccaacgga tgcccgatgc ttgcgcctct
     1741 ccgacaaaag gcccgtccga agaagcaagg atgatgtcgt gcccgcgccg tttgagcagc
     1801 cgcccgaggc ggacggtggc ggtttccgtg ccgcccaagc ccgacataga agtggtcagg
     1861 atgattttca taatggaaac ccttgttttt tcaaataatg aaacagtttg tgcaaatctt
     1921 tccagtggcg caggatgcag cctgccagat ataccgccca tacgccggca aacagggggt
     1981 agtttgccgg tgtgccgaag caggtgtagg ccgccgagga ggtcaggcag agcaatgtgt
     2041 gcgtataaag cggcaggcgt ttgagcggct gccacaggcg acaggagctt tctgtcttga
     2101 aaacaaaaaa caaccagaat gaggtggcac aggcaaccgc cgcgccgcgc gcgccgccgg
     2161 acggtacggc aagccccagc agcagcaggt ttgccgccag cgcgcccaag gtggcgagcg
     2221 cgatcgggcg tgtttttcgg acgacgttca aaccgatgcc gctgatttct accagcgtgc
     2281 aaaacagcgg cggcagcata cacgatacga cgataaaccg gacggcagcg tagttttccg
     2341 gcagcaggag ggaggcgagg ggcgagaaaa tgccggtcag gcagagggcg gaggcaagca
     2401 gggcggcggc ggattctgcc gttgccgaga ggcgggcggg cggggcgttt tcttcgattg
     2461 cgcggaaaat atacggtgtc cagaccgttg aaaagatgct ttggaacaat aatgccgccc
     2521 cgccgaacga aatacccatc gaataaacgc cgagctgttc cagaccggca tattttttca
     2581 ggaacaaacg gtcggcggat gccagccccc aataggcgat gctgctcagt gcgatcggta
     2641 tgccgtagcg cagcccccgg tgcaggacgg cgggcgaaaa cggtgcgtgc cggacggcct
     2701 tcagacggca tcggttttga aacagcaaaa aggcggcggc ggcaaggttt gccagcgcgt
     2761 aaacggcggt caggacggcg gtgttcgccg gaaagtgcag cagcccgacc gtcagcggca
     2821 gcaggatggc gagcttgggc acgagttgcg cggacgaaaa ggcaagggcg cgcccttcca
     2881 tacgcaaaac cagtaagaga aagcggatgg gcaggaagct cagttcaaac agcaccagcc
     2941 cgatgccggc ggcggcatcg tcgagcgaaa acaggatttc agacggcagg gacgggcggg
     3001 aaagcagcag ggcggctatc gcggcggcag acagcagcgg cggcaggaac agggttttga
     3061 acaaggtgtc tttgtcggcg gcggcatagt attcgcggac gtatgcctga tccagcccga
     3121 ggcacaatac cgacaccgtc agtcccgccg ccgtctgcat cagcacgatg cgcccgatgt
     3181 cgtcggcggg gaaataccac gacagcagcg gcaggatgat gacggctaaa accgcgctgc
     3241 cgatcgagcc tgccgcgtag ccgaggattt cttttgtgtc catttttgat gtccgggcgg
     3301 cggcgggatg ctgcctgtgc cgtctgaagc ctttcttgat cggaatttga cggctttcag
     3361 tccgttgcgg ctgccggcgg ggtgcggcag tccgggttgc gcttttccgg gcgggcggcg
     3421 gtctgaacgg ggccgttttt tatcggcgtt attatatagt gaaacggcgc aaacccttta
     3481 aaaggcgtta tcgtttttcc ggaacacggt tttgatgtcg tgtccgagga tttcgcttga
     3541 aacgggtgtc cacaagggcg gtgcggaaag ggcggcgcgg ttttcgggca gggaaagcag
     3601 gtctttgccg ctgccgagga ttttgggcga acggtacagc acgatttcgt ccgccagatc
     3661 ttctgccaga aatgcggatg tgagttcgga gcctgcttcg accatgattt ctccgaaacc
     3721 ttcgtcggca aggaggcgca tcaggtggtt caggtcgatt ttgccgcctg ccgtttcaga
     3781 cggcatcagg atgcggatgt gtgcgtgttc ccgatagggg cggagtttat cttcatcgcg
     3841 ttccaaggtg gcgatgtagg ttggagattg tccgtcggtc agaaggtggc tgtccaaggg
     3901 caggcgcagg cggctgtcta acacgatgcg tgcgggttgg cgcaaagttg gaaaagcgcg
     3961 gacgttgagc cggggattgt ccgccaacac cgtgccgatg ccggtcagca ccgcgcagct
     4021 ttcggcacgc aaaacctgta cgtcggcacg cgcgtcttcg ccggtaatcc aaaagctgct
     4081 gccgtctgaa agggcggttt tgccgtccag cgaaacggcg catttgaggc ggacgaaggg
     4141 gcggcggcgt tcgatgcgcg acaggaagcc tcggttgagt tcccttgcct gatgttcgag
     4201 taaaccgcat tccgtcttga tgcctgctgc ttcgagcagg gcaagccctt tgcctgcaac
     4261 cagcgggttg gggtcgcgca tggcggcgac gacgcgtgtt acaccggaac ggagcagagc
     4321 ttcggcgcag ggcggtgtgc gtccgtaatg gctgcacggt tcgagggtaa caaaggcggt
     4381 cgcgccttgt gccatttcgc ccgcctgacg cagggcgtgg acttcggcgt gcggttcgcc
     4441 cgctttgacg tggaagcctt gcccgacaat ttggctgccg tgtgcgataa cgcagccgac
     4501 gcgcggattg ggcgaagtgg aaaaacgtcc caaagcggca agtcggaggg cggtttccat
     4561 catggatata tctgtgtccg aaaacatagg gataccgtat cagtatgggt tggggagaat
     4621 caggctttgc ggtctgtttt gacggcttgc gccagccacg aggcaaagtc tgccggattg
     4681 tcgaagcgtt tgtgcagggc ggcgaaacgg acggcggctt ccgtgttgtg ctcaagcagc
     4741 tcttttagca ccatgtcggc aagtgcggca gaggggatgt cgcgctgacc cgaagtgtag
     4801 agcctgtgtt tcgtcaggcg gacggtttcg tcgatctgtt cgggtgtcag ggcggatttt
     4861 cgggcggcgg cggtcaggtc gttgcggagg cgttgtgcat taaagggcga acgttttttg
     4921 tccggaccga tgacggcggg cattttgagt tcggcggttt cgagcgtgcc gaagcgtttg
     4981 ccgcagctgg ggcagtggcg gcggcggcgc acggcgttgc gttcttccat caggcgcgaa
     5041 tcggcgacgc gggtgtcggg gtgggcgcaa aacgggcatt tcatagcgtt tcttcctgat
     5101 gccgtctgaa cgtcaaaccg atacgccggc ggcgcgggcg atttccagac cttcttcggc
     5161 actcatatag acggggtttt cgggacggtc gtgtcggacg atgttgcctt cgcggaacat
     5221 gaccagttcg tccacggcaa gttgggacca tgattcgtcg cgggtcagtg gcagggtggc
     5281 gataacggcg acgcggtcgg agggcgtggt tacttcggca aaatcgacca tcacgtcgtc
     5341 gtcgagcagg cgcgccttgc cgaacggggc ttggcggacg atgtagtgca gcagtgtgct
     5401 ggcgtgggca aacagggcaa tgccgtctga aagcataaag ttaaacagcc cgaacttgcg
     5461 gatttcgtgc gtcagccccg caatcgcgtc aaacagcgtg tcgtcgtcgg gacgggcggc
     5521 aaagcgggtg cgcaggcggt tgaggatgtg gcagaacgcg cgttcggaat cggttgtgcc
     5581 gacggggtgg aaaaattcgc cctgttcggg gaaaaaatca atcaaatgtc cgttgtgggc
     5641 aaacagccag tagccgcccc acatttcgcg cataaaggga tgggtgttcg ccagcgaggt
     5701 ttgtccttgc gatgctttgc ggatatgtgc gatgacgttt tccgatttga tttggtaggc
     5761 acgcacgagg tcggcgacgg gggaatttgc gctcggcttg tcgtcgtgga acaggcgcac
     5821 gcctttgcct tcgaaaaagc cgatgccgaa accgtcggca tgatggtcgg taatgccgcc
     5881 gcgccggcgg aagccttcaa aggaaaacat aatatcggtc ggcgtattgc agttcatgcc
     5941 cagcagttga cacatagttt gtccaaatga ttcagatggt cgcaagtatt cggattatac
     6001 cccgaactga aaatgccgtc tgaaatacgg cttgttcccc attattccca cgaaaacaga
     6061 aaacaaagac ggaaacttaa gattccgtca ttcccgcgca ggcgggaatc tggaatttca
     6121 atgcctcaag aatttatcga aaaaaaccaa aaccc
//
LOCUS       DAWEWP010000116         6126 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWEWP010000116 DAWEWP010000000
VERSION     DAWEWP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.116"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(160..2088)
                     /gene="dnaK"
                     /locus_tag="WGN53_001578"
     CDS             complement(160..2088)
                     /gene="dnaK"
                     /locus_tag="WGN53_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ0140552.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTSD
                     IDDVILVGGQSRMPKVQEAVRDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2296..2568)
                     /locus_tag="WGN53_001579"
     CDS             complement(2296..2568)
                     /locus_tag="WGN53_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140553.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2754..3440
                     /locus_tag="WGN53_001580"
     CDS             2754..3440
                     /locus_tag="WGN53_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0140554.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRNTKNLSVISVKGDSMEGVLNDGDSILVN
                     HSENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNHPADDVEII
                     GRVEWFGRTI"
     gene            3572..3910
                     /gene="erpA"
                     /locus_tag="WGN53_001581"
     CDS             3572..3910
                     /gene="erpA"
                     /locus_tag="WGN53_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ0140555.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3927..4073
                     /locus_tag="WGN53_001582"
     CDS             3927..4073
                     /locus_tag="WGN53_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140556.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4070..4534
                     /locus_tag="WGN53_001583"
     CDS             4070..4534
                     /locus_tag="WGN53_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0140557.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPKVDCGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWEKRAELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     GDGEIPEYLELHSEREDDISAPSQAEPPPRNIKPLVFGLLIAFLGYLLGKIF"
     gene            4569..6080
                     /gene="ubiB"
                     /locus_tag="WGN53_001584"
     CDS             4569..6080
                     /gene="ubiB"
                     /locus_tag="WGN53_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ0140558.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAVWVERLFADGKRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1382 a         1360 c         1644 g         1740 t
ORIGIN      
        1 actatataaa aaatttccag agaaccgata caacagttgg aacttgggtt tgggaatatt
       61 acggtagatg aacttggaac ctctgttatg ctatggtctt ttatctcaat tgaaaaaagc
      121 gcgaatcaaa cggttcgcgc ttttttcaga cggtattaat tattttttgt cgtcttttac
      181 ttcttcaaag tcggcatcta cgacatcatc gtctttcttt gcagaagcat tggcttgttc
      241 gctttcgcct gcttgggctt cagcttgtgc ttgagcgtaa accatttccc ccagtttttg
      301 gctggctgcg cccagcgcct cggttttggc atcgatagcg gctttgtcgt cgcctttaac
      361 tgcttcttcg gcttctttca gcgcggcttc gattttttct ttctcggctg cgtcgagttt
      421 gtcgccgtag tcggccaaag attttttcac agagtgaatc agggcttcag cttggttgcg
      481 ggaagcgacc aattcagtca gttttttatc ttcctcggca ttggcttcgg catctttcac
      541 catgcgttcg atttcttctt cgctcaaacc tgaagaacct tggatggtga tgttggctgc
      601 tttgccagtg cctttgtctt tggcggaaac gtgcaggatg ccgttggcgt cgatgtcgaa
      661 ggtgacttcg atttgcggca taccgcgcgg tgcgggtgcg atgtcgccca agttgaactg
      721 acccaaagat ttgttggcag aagcgcgttc gcgttcgcct tgcagtacgt ggatggttac
      781 tgcgctttgg ttgtcttcgg cggtagagaa cacttgcgac gctttagtcg ggatagttgt
      841 gttcttttga atcagcttgg tcatcacgcc gcccatggtt tcgataccca aggacagagg
      901 ggttacgtcc agcagcaata cgtcgctgcg gccgccgctc aatacttcgc cttggatcgc
      961 tgcgcctacg gcaacggctt cgtcagggtt cacgtctttg cgcggttctt tgccgaagaa
     1021 atctctaacg gcttcttgta ctttcggcat acgggactga ccgccgacca aaattacgtc
     1081 gtcgatgtcg ctggtgctca agccggcatc tttcaatgcg gtgcggcaag gctcgataga
     1141 gcgggcaatc aggtcttcaa ccaggctttc gaatttggcg cgggtaattt tcatcgccaa
     1201 gtgtttcggg ccggttgcgt ccatggtgat gtacggcagg ttaatttcgg tttgctggcc
     1261 gctggacaat tcgattttgg ctttttcggc agcttctttc aggcgttgta gagccatcac
     1321 gtcttgtttc aaatcaatgc cttgttcttt tttgaactcg gcgatgatgt ggtcgatgag
     1381 gcgttggtcg aagtcttcac cgcccaagaa ggtatcgccg ttggttgcca atacttcgaa
     1441 ttgtttgtcg ccgtcgaggt tggcgatttc gatgatggaa atatcgaaag taccgccgcc
     1501 caagtcatat acggctactt tgcggtcttt gttgtcgcct ttgtccatac cgaatgccaa
     1561 agcggctgcg gtcggctcgt tgatgatgcg tttcacgtcc aaaccggcga tacggcctgc
     1621 gtctttggtg gcttgacgtt ggctgtcgtt gaagtaggca gggacggtaa tcacggcttc
     1681 ggttactttt tcgcccaagt aagcttcggc ggcttctttc attttacgca ggacttctgc
     1741 ggaaatttga ggaggagaca gctctttgcc ttgtgctttt acccatgcgt cgccgttgtt
     1801 ggctttgatg atttcgaaag gcatagattc gatgtcgcgt tggacttctt tgtcttcaaa
     1861 tttgtggccg atcaaacgtt tggcggcgta aatagtgttt ttggcgttgg ttaccgcttg
     1921 gcgtttggca ggcgcaccga cgaggatttc gccgccgtcc aaataagcga taacggacgg
     1981 cgtggtgcgt gcgccttctg cgttttcgat cactttggtt tgaccgtttt cggaaatggc
     2041 caaacaagag ttggttgtac ctaagtcgat accgattact tttgccatgt ggataatcct
     2101 atttgatttt gcttattttg agaaatatgt tggaacattt tgtcccgatg ggctgtaaat
     2161 agggcgggcg gcgggctgtt tcaagctaca gcatggctat aagtatataa ctttatgaat
     2221 atattggttt tatatttgat ttaatacatt tggctccaat gcattcaagc ataatgtttc
     2281 aaatggcagg caggtttatt catagacgat gccggcgagc atttcctgtt cgttcaagtt
     2341 gccgtactct ttttcccagt cgtgtgaaga ctcgatgatg tcgcattctt tggaaaggga
     2401 gacttgttct gcatccatat ctttggcgtt cagtatgttg aattgttcgc atagggatgc
     2461 ggataaagtg atgtcaggct gtttggcttc agaacggttt tcttggaagg caaagcagaa
     2521 tgcggtaaat gccgcagcat agataagata tttgccggtt ttcttcattt tctatccttt
     2581 ttctgtcaac tcaggattaa acctatggaa aatcttaaaa attatgtatt gagtaagaaa
     2641 aatcataatt taaatttagt ttatcatagt tgttccgttt ttgggatagc taaggtaaaa
     2701 tatatttcat gtttacttta gatgattgaa tgaaggggag cggaaggata tttatggaaa
     2761 cctttaaaga cagactggtt tttttatgga aaagcgaagc gaagcaggca aaaatcgcat
     2821 ccgatattga aatgacgatt gcgggcttca gcaggatatg gaatgaaggc ggtctgccga
     2881 agtctgaaac attaaaaaaa atcaagcagt tgaaggggtg tagtatcgat tggctgctga
     2941 ccggggaggg taatccgttt ccggatgaag ccccaaaaaa atcccttgct tacgatacct
     3001 tgggaaatga agtcgatacg gacgagtttg tctttgtgcc gagatatgat attcgggcgg
     3061 ctgcgggata cgggcagttt gtcgatcatg aggaaccggt atttacaatg gcgttcagac
     3121 ggcattggat tgagaattat gttacccgca atacgaaaaa cctgtctgta atttccgtca
     3181 agggggattc gatggagggg gttttgaatg acggcgattc gattttggtc aaccatagcg
     3241 agaatacgcc gagggacgga ctgtatgtgt tgcggattaa tgaaaatctg ctggttaaac
     3301 gtttacagat tgtaccgggc gggataatca acgttatttc cgccaacgag gcttaccctg
     3361 cttttgaaat taatttgaat catcctgccg atgatgtgga aatcatcggg cgggtcgagt
     3421 ggttcggcag gacgatttga gtttggggct tgaaatcggg agcggtcaaa cttatctgta
     3481 aggcagttcc ttttcaggga acaagccccc gcttgccttt gaagggggtt tgagagagaa
     3541 tgcagaaaat attatattaa ggaataacac catgtcggat gaaagcccta ttatttttac
     3601 tgacagctgc tgtgccaaag ttgccgattt gattgccgaa gaaaacaatc ccgatttgaa
     3661 attgcgggtt tttgtcaatg gcggcggctg ttcgggtttc cagtacggat ttacttttga
     3721 tgaaatcaaa aacgacgacg attttgaaat tgagaaaaac ggtttggtct ttttggtcga
     3781 tccgatgagc tatcaatatc tggtcggcgc ggaaatcgac tatacggaaa gtttgcaggg
     3841 ttcgcaattc gtcatccgca atccgaatgc ggaaacaacc tgcggttgcg gatcgtcgtt
     3901 ttccgtatga ccgcttggtt tgtgtgatgc cgtctgaacg ttcagacggc atttttactt
     3961 ttggaaaata tattatcggg atgaattcac atataatccg attgtttgaa gatgaatcgg
     4021 gtttcccgaa aggaacgggc ggaacggtat caggcgtatt tgttccctta tgattgagat
     4081 gagtaaagat taccgaaacg atttgtacga tgtatatgtt tcctacccgc ccaaagtgga
     4141 ttgcgggctt atccgggagt gtctcaggga gaatctcggc gaagaaaagg cggaaggatt
     4201 gattgagtcc ctcgattcca aaccgcaagt gctggttgag gaaaaatgta cctgggaaaa
     4261 gcgggcagag ttgcacgatt atttcagcta tttgggtttg gatattatta ctcggagata
     4321 tatggagtta gaaacggtcg tgccgccgga ggaaggggag ggcgaaggag aaggcgggga
     4381 tggggaaata cccgaatatc ttgaacttca tagcgaacgg gaagatgata tttccgcacc
     4441 ttcgcaagcc gaaccgccgc cccgcaatat caaaccgctg gttttcgggc tgctgatagc
     4501 ctttttgggc tatctgctcg gtaagatttt ttgattgtcc gataaatgct gtattcggga
     4561 ttttatatat gaaatggttg aaacgcctga cggttattgt cgggactttt taccgctatc
     4621 ggctggcagg tctgtgcgct tcgctgatgg gtagcggttg gatatgcgct ctgctgaaaa
     4681 tgatgccgca gtcgtccaaa ttgaaaaacg aaccgcctgc cgtccgtctg cgccttgcct
     4741 tggaaagcct ggggccgatt ttcatcaagt tcgggcaggt tttgtccaca cgccctgatt
     4801 tgattccgca cgattacgcc gtcgaactgg caaagctgca agacaaagtg ccgccttttg
     4861 acgcgcggct ttcgcgtgaa caaatcgaaa aatcgttggg tcagtccatc gaaaagctgt
     4921 atgcggaatt tgaaaccgag cccgtcgcca gcgcgtccat cgcccaagta cacaaagccc
     4981 gcctgcattc gggcgaacga gtggctgtca aagttttgcg ccccaacctt ttgcccctta
     5041 tcgaacagga tttgtcgctg atgcgtttcg gcgcggtttg ggtggagcgt ctgtttgccg
     5101 acggcaagcg tctgaagccg ctcgaagtgg tggcggagtt cgacaaatac ctgcacgacg
     5161 agttggattt gatgcgcgaa gccgccaatg ccggacagct cgggcgcaat ttccaaaaca
     5221 gcaatatgct gattgtgccc aaggtgtttt acgactactg caccagcgac gtgctgacaa
     5281 tcgaatggat ggacggcacg ccggttgccg acatcgccaa actcaaagca gacggcatcg
     5341 atttgcacaa actcgccgat tacggcgtgg aaatcttttt cacgcaggtc ttccgcgacg
     5401 gctttttcca cgcggatatg caccccggca atattttggt tgccgccgac aaccgctaca
     5461 tcgccctcga tttcggcatc gtcggcacgc tgaccgatta cgacaaacgt tatctcgcca
     5521 tcaatttcct cgcctttttc aaccgcgatt accggcgcgt cgccaccgcc cacatcgaat
     5581 cgggctgggt gcctgccgac acgcgtgcgg aagaattgga agccgccgtc cgcgccgtgt
     5641 gcgaaccggt gttcaacaaa ccgatttcgc agatttcctt cggcttggtg ctgatgcgcc
     5701 tgtttgaagt cagccgccgc ttcaatgtcg aaatccaacc gcagcttgtg ctgctgcaaa
     5761 aaacgctgct caacatcgaa ggcttgggcc gccagctcga tcccgatttg gacttgtgga
     5821 aaaccgccaa accgtttttg gtgaaatgga tgaacgggca ggtcggccct aaagcccttt
     5881 ggcgcaacct caaaaacgaa gcccccgact gggcgcaaat catcccttca ttgccgcgca
     5941 aaatcagtgc gttgattgat gaaaaccgcc agcaggaaat gcgtgatgcc tatatccatt
     6001 tggtcaaagt gcagcagcgg caaagcctgt ggctggctgt gattgcggtt gttttgctgc
     6061 tgattttgct tttgaaatag gctttgtccg aatcatcgcc cgactccgcc cgtttataag
     6121 gaaatc
//
LOCUS       DAWEWP010000117         6083 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWEWP010000117 DAWEWP010000000
VERSION     DAWEWP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.117"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            965..1201
                     /locus_tag="WGN53_001585"
     CDS             965..1201
                     /locus_tag="WGN53_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ0140559.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGFGVQFSKPTFGFSKKW"
     gene            2242..2379
                     /locus_tag="WGN53_001586"
     CDS             2242..2379
                     /locus_tag="WGN53_001586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140560.1"
                     /translation="MNTETIYATVFCILAATISGLLVKFNVIKIETSINSKFMLLGIS
                     I"
     gene            2424..3689
                     /locus_tag="WGN53_001587"
     CDS             2424..3689
                     /locus_tag="WGN53_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ0140561.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            3692..5779
                     /locus_tag="WGN53_001588"
     CDS             3692..5779
                     /locus_tag="WGN53_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ0140562.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAIFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(5861..6034)
                     /locus_tag="WGN53_001589"
     CDS             complement(5861..6034)
                     /locus_tag="WGN53_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140563.1"
                     /translation="MSWWEFREFWGILQRSQTVKLAQKWEQEQKKQQIQQKKETEKSP
                     KHKASRDDWEMER"
BASE COUNT         2010 a         1055 c         1082 g         1936 t
ORIGIN      
        1 aaaattccat gtttgtgtac tgaaatgctt caaaacacca aaccaagttt cgttttctaa
       61 aatacgaaac cattactgct gcctaaattt ttttggattg ctaaattatg gcagtatgat
      121 tttggatttt aaattgaaag gcaagaaaaa tgtcaaaaaa tgatgtagtt aaagtaattg
      181 gtatattccc cctattgtcc gaacaataga gcagacttcc cggcaggtca aattgtttta
      241 aaacacggcg aacacaaaaa ggttaatcac ggctttggtg tgattcacat tttggcagaa
      301 cacaaagcag atttaacgcg gtttggatta agccacgatg aaacaggtgt aaccctttta
      361 tgtggaaatg atttttcaat caggcgcaaa aatttttagt gaatttagtg atgtaagggg
      421 caatcatcgt cctatgattg tctattctcg gattgggaca gtcgttttag aacgcaaaga
      481 agttgagggg gaaagtgtgt attcagttgt taccgcgttt gggcgaacaa gccctcgtgg
      541 tacacaaatt ggtacaatgc cataaaaaga caaaagcacc caataatttc aggtgctttg
      601 tgtagtagcc gcagcctctc cttagcttga tagggtcgga taccaatctg acgattgagc
      661 tactgaaatt ggttagcgac aaccaacctg cccacatcag aacgcccgtt cgctggtttg
      721 tacgtcctga aaaagctctt gcattaagtt aatcataatg ggaaatttaa attttttcaa
      781 tgcttactta aacaaaagcc ccactccacc attaggagtt tctttttcag tatacaagta
      841 aatattttta aaatattgat ttaatttaaa ataaaatact tgcaaaaaaa aagtattaaa
      901 ttaaacttga gaaaggttaa ttctgattta catttccaac catacttctt tacaggagaa
      961 aatcatgaaa gagttacaca cctctgaatt agttgaagtg tcaggtggca aattccatat
     1021 ctctgcacag ggtggcggca acctaggtaa aaaagatatg gttgctgttg gtaaaattgg
     1081 tgcttcctat tcccctaaca atagtggagt agagttttct gttagcaagc aatttggata
     1141 tgtacaaggt tttggtgtac agttttcgaa acctactttt ggttttagta aaaaatggta
     1201 agattttttg ttttatcctt tctgacatta ataaatctat gctcattaag cgcatgcaat
     1261 agccacttta caggaaatat caatccatta ggtactcaca ataaagttgc taatcccagt
     1321 tgtgccaata gtgccaatag tcatatcaga caacccagta gtaaaaacta tgatccaact
     1381 gaatatagtg cttggttaca gcatatgcat gattgcaaat aatgagtaac gatgaaaatt
     1441 tacttttttc tcaaccacac ttaacaaagg tgaatattat gcaagttttg actttgaatg
     1501 aaatttaaca agtttctggt gctgcttgta actggcgtga tttctcaaaa aataccattg
     1561 gtagtgcatt aggtggagca gctggtgggg caattgttgg ttcatttgca ggtggtattg
     1621 gtgctattcc aggtgcgaaa ttcggagcta ttggtggtgc aatcactggt gctgtacaat
     1681 atggaagcac ttgttggtgg taatattcct taataaaact agggtatttt gatattttct
     1741 attcaaaata ccctagtttt tcataagaac ttaaatacaa aaaggaacaa ataatgaaaa
     1801 aatatagtga ttattttaaa tatttaatct tttttttgat tttactccca acaaattatc
     1861 tcgtatctca ttatgtggta caaacctcaa tgagtatgtt aagcatttta agttcttcta
     1921 taataacaac ttgccttttt tttgttttta caaatttatc ccaatcaaag aaacataaat
     1981 aagtacacat gtctaacaat cactcatttt tcagaccaga agtctttgta gctcaacgga
     2041 acaagtggac aggaccagta ggctgggttg gcgcaattgg agctggtatt ttctctgctg
     2101 ctggcggata caatatcggt cgtggcatga tgaagccata agataattac atcattaagg
     2161 aaaaggtaat ttcagttaca gcaatatgta ttgaagttac ctttttctat ttagattgaa
     2221 caattttgaa agagaaaaat tatgaatact gaaaccattt acgccactgt cttttgcatt
     2281 ttagctgcaa ccatttctgg attattggtt aaatttaatg taattaaaat agaaacatca
     2341 atcaatagca aatttatgtt attaggcata agtatttaat tattggtgtt tttctatcca
     2401 ttttttttaa gaaataataa taaatgtccc acttattccg aaaagaagtc tttgtagccc
     2461 aacaaaataa gtggacaggt caggttatct tgacccgtcc attctctttt ttatttctga
     2521 ctttttgcgc ttttctcatt gctctgtgta tcattatctt tttgattttt ggtagctata
     2581 ccaataaaac aaccgttgaa ggtcaattac ttccaactat gggggtggtt cgtgtttact
     2641 cttccgatat cggcacgatt acgcataaat ttgttgaaga tggtaacttt gtcaaagctg
     2701 gcgaaccatt gttcaaactt tccacatcgc gttttggcga aaaaggaaac gtacaagcca
     2761 aattggcagc agaagccaac cttaaaaaaa ctttggcatt acaagaattg gaacgtttaa
     2821 agcgcattca tcaaaatgag caaaaaaatg ttcataacaa cattcatcgt ttaaacaatc
     2881 aattagagaa tattaaacag caaattacag ggcaaaatcg tcaaattcgt ttagcggaaa
     2941 aaacccttaa caagaacaag tttttagcca gtcaaggcgc agtatcccaa caagataaga
     3001 tgaccgccga aagccattta ttggaacaac gctcacgttt ggagagccta aaacgtgaac
     3061 aaaataatgc aatcagggaa cttgatgaac agaaaatcac attaagcagc ctgcctgaac
     3121 gccataaaac cgaattgagc caactcaacc gtgcgattac ggaaatgaac caagaaattt
     3181 tggattttga tttgaaatcc gaacaaacca tacgagctag taaatcaggt tatatttcaa
     3241 caattaatgt tgatataggg caacaagttg aaccgtctaa attgctgtta agcattgtcc
     3301 ctgaacaaac tgaattggtc gccaatcttt acatacccag taaagctgtt ggttttatta
     3361 aaccgaaaga taaagttgtt ttacgttacc aagcgtaccc ttaccaaaaa tttggacatg
     3421 ccacaggaga aattatttca gttgccagaa ctgctctcgg taaacaagag ctatcaggtt
     3481 taggtatcat tttcactaac ccaaccttat taaatgaacc tgcctatctt gtgaaagtta
     3541 aattggaaaa acaaacgatt aaagcatacg gagaaaacaa gccgcttcaa attggcatga
     3601 ttttagaagc agatattctc catgaacgaa aaaaattgta cgaatgggta cttgacccac
     3661 tttacagcat ttcaggaaaa atcaattaaa aatggattat ttatcaagac tgtcctttgg
     3721 atttaacaaa aagctacctg tcattctgca aacagaagtt gctgaatgtg gtttagcatg
     3781 cctgacatcc atcttgtcct attatggctt tcacactgat ttaagaacgt tacgccaaaa
     3841 atacaccctg tcattaaagg gcgcaaatct tgcagacatc atgagatttg gcaatgaaat
     3901 gaatttaacg ccacgagctt tgcgtttaga gttagatgag ctgtcaaatt tacaactacc
     3961 ctgcattctc cattggaact taaaccattt tgttgtactt tgttccattt ccaaagacag
     4021 tatcgtcatt atggaccctg ctgtcggtat gcgaaaaatc aaaatggacg aagtttcaca
     4081 aaaattcaca gggattgccc tagaattatt ccccaatacc cattttgaag agaaaaaaga
     4141 aacaaagaaa atcaaaatat tatctctatt aagggggatt tcaggcttaa aacgctcttt
     4201 aattcaaatg cttatattag ctatttcttt ggaagtcttt gcattggtta gtccattctt
     4261 tatgcaatgg gtaatagacc atgtcattgt aactgctgat aaaaatttat tactgaccct
     4321 tactttggga tttggtttac tggctatcct gcaacagtta attagcctgt tacaagcatg
     4381 ggtaggtatg cacctatcta caactcttaa tttacaatgg aaagccaata tatttaaaag
     4441 gttacttgac ttacctaatg actatttcag taaacgacat ttaggagatg tgatttcaag
     4501 atttggttca atagatcata tccaagaaac actaacttct actttttttg ttttagtttt
     4561 aaatagctta atggctattt ttactttcgt gttaatgaca atttacagca ctcaattatc
     4621 gctgattgtt cttttaacac ttgttttgta catactaatt cgttggcttg catattaccc
     4681 attaagaaat gcaacagaag aaaatattgt tcatgaagcc aaacaaaact catatttcat
     4741 ggaaaccatt cgtggtatcc aatcagttaa attatttgat aaacattatc aaagacatgg
     4801 cacttggatg agcctatttg tgaatacagt caataccaag ctgacaacag ataaactctc
     4861 tgctttattt gaattttcaa ataaactgtt gtttagcatg gaaaatatta tcataattta
     4921 tcttggtgca agcgcaattt tagatggttc atttacagtc ggtgttctga tggctttttt
     4981 ggcttataaa gggcaatttg aaagcagaac agcttctctc gttgaccaat acatccaaat
     5041 caaaatgtta gggcttcatg ctgaacgttt ggctgacatt actttaaatg aaacagaaac
     5101 tgaaattatt aagtataatc atatacctaa attagataat gaacaactgg ttcttaaagt
     5161 tgaaaacgtc tcattcagat atgctgataa tgagccatat ctttttgaaa acattaattt
     5221 ggaatttaaa gataatgaag cagttgtttt aacaggacaa tctggtcggg ggaagtccac
     5281 tttgttaaac attttaacag gtagcctaaa acctgaaact ggtacagtta gtattaatgg
     5341 gcatgatata tatcaagttt ctccatcctt tattagggga ttgagcggga ttgttcgcca
     5401 agatgatgtc ctttttgcag gttctattgg ggaaaatatt tcattttttg atgaaagccc
     5461 aaatatggag ctcattgaac aatgtgcaaa aatggcacaa atacatgacg atatacttaa
     5521 aatgccaatg ggctatgaga ccttgattgg cgatatggga aatatcttat caggtggaca
     5581 aaagcagaga gttatcttgg ctcgtgcatt gtataaacga cccaaaattc tatttttaga
     5641 cgaagcaagt agccatttag atgtagaaaa tgaacaaaaa attaaccata acctaaaaag
     5701 tcttggtatt atgaaaataa tggttgcaca ccgccaagaa acaattcaat cggcagataa
     5761 aattctgaat ttaggttgaa cagaacaaga cttcattttt ctttaacaaa aagtgaagtc
     5821 ttttttcaaa taatttaata gaatacatga aaatagcggt ttaacgttcc atttcccaat
     5881 catcacgact ggctttgtgt tttggcgatt tttcagtttc ctttttctgt tgaatttgtt
     5941 gttttttctg ctcttgttcc catttttggg ctaatttcac ggtctgagac ctttgcaaaa
     6001 ttccccaaaa ttccctaaat tcccaccaag acatttaggg gatttctcat gagcaccttc
     6061 ttccaacaaa ccgcccaagc cat
//
LOCUS       DAWEWP010000118         5960 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWEWP010000118 DAWEWP010000000
VERSION     DAWEWP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.118"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            28..1383
                     /locus_tag="WGN53_001590"
     CDS             28..1383
                     /locus_tag="WGN53_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ0140564.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLFYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKSY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLIITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMMGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1387..2433
                     /locus_tag="WGN53_001591"
     CDS             1387..2433
                     /locus_tag="WGN53_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ0140565.1"
                     /translation="MSDTPATPATPATPATPATRDFGLIDGRAVTGYVLSNRRGTRVC
                     VLDLGGIVQEFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDIN
                     GRTYRVEANEGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDI
                     SYRLDEDNRLTVSYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKL
                     PVSTVSDDLEVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRR
                     RRISIYSDRNGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGD
                     TREATIAYGIESLS"
     gene            2667..4925
                     /locus_tag="WGN53_001592"
     CDS             2667..4925
                     /locus_tag="WGN53_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ0140566.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFDVCKFLSVAQKELALIRWEAVSIDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDDRSYIATQTVTNPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEAEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTAALEK"
     gene            4938..5603
                     /gene="pgmB"
                     /locus_tag="WGN53_001593"
     CDS             4938..5603
                     /gene="pgmB"
                     /locus_tag="WGN53_001593"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ0140567.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGISIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVSEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLET
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKASGALPIGVGKAEDLGSDIPLVSGTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(5813..>5960)
                     /locus_tag="WGN53_001594"
     CDS             complement(5813..>5960)
                     /locus_tag="WGN53_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0140568.1"
                     /translation="SFGTLHRKFRYARSTYFGLIKVSAQSHLKAMCLNLLKAANRLSA
                     PAAA"
BASE COUNT         1342 a         1735 c         1602 g         1281 t
ORIGIN      
        1 ttttcaaatc aaaaaaggat ttaccttatg tcggaatata cgcctcaaac agcaaaacaa
       61 ggtttgcccg cgctggcaaa aagcacgatt tggatgctca gtttcggctt tctcggcgtt
      121 cagacggcct ttaccctgca aagctcgcaa atgagccgca tttttcaaac gctaggcgca
      181 gacccgcaca atttgggctg gtttttcatc ctgccgccgc tggcggggat gctggtgcag
      241 ccgattgtcg gccattactc cgaccgcact tggaagccgc gtttaggcgg ccgccgtctg
      301 ccgtatctgt tttatggcac gctgattgcg gttattgtga tgattttgat gccgaactcg
      361 ggcagcttcg gtttcggcta tgcgtcgctg gcggctttgt cgttcggcgc gctgatgatt
      421 gcgctgttgg acgtgtcgtc gaatatggcg atgcagccgt ttaagatgat ggtcggcgat
      481 atggtcaacg aggagcagaa aagctacgcc tacgggattc aaagtttctt agcgaatacg
      541 ggcgcggtcg tggcggcgat tctgccgttt gtgttcgcgt atatcggttt ggcgaacacc
      601 gccgagaaag gcgttgtgcc acaaaccgtg gtcgtagcat tctatgtggg cgcggcatta
      661 ctgattatta ccagtgcgtt cacaatcttc aaagtcaaag aatacgaccc ggaaacctat
      721 gcccgttacc acggcatcga tgtcgccgcg aatcaggaaa aagccaactg gttcgaactt
      781 ttaaaaacgg cacctaaagc gttttggacg gttactctgg tacagttttt ctgctggttc
      841 gccttccaat atatgtggac ttactcggca ggcgcgattg cggaaaacgt ctggcacacc
      901 actgatgcgt cttccgtagg ttatcaggag gcgggcaact ggtacggcgt tttggcggcg
      961 gtgcagtcgg ttgcggcggt gatttgttcg tttgtattgg cgaaagtgcc gaataaatac
     1021 cataaggcgg gttatttcgg ctgtttggct ttgggcgcgc tcggcttttt ctccgttttc
     1081 ttcatcggca accaatacgc gctggtgttg tcttatacct taatcggcat cgcttgggcg
     1141 ggcattatca cttatccgct gacgattgtg accaacgcct tgtcgggcaa gcatatgggc
     1201 acttacttgg gcctgtttaa cggctctatc tgtatgccgc aaatcgtcgc ttcgctgttg
     1261 agtttcgtgc ttttcccgat gatgggcggc ttgcaggcca ctatgttctt ggtagggggc
     1321 gtcgtcctgc tgctgggcgc gttttccgtg ttcctgatta aagaaacaca cggcggggtt
     1381 tgagcgatga gcgatacccc cgctaccccc gctacccccg ctacccccgc tacccccgct
     1441 acccgcgatt tcggcctgat cgacgggcgt gccgtaaccg gctatgtgct gtccaatcgg
     1501 cgtggtacgc gtgtctgcgt gctggacttg ggcgggattg tgcaggaatt ttccgttttg
     1561 gcagacggcg tgcgcgaaaa cctcgtggtg tcgttcgacg atgcggcttc ctatgcggac
     1621 aatccgtttc agattaacaa gcagataggg cgcgtggccg gacgcatccg cggtgcggcg
     1681 ttcgacatca acggcaggac ttaccgcgtg gaggccaacg aaggcaggaa cgcgctgcac
     1741 ggcggttcgc acgggctggc cgttacccgt ttcaacgcgg tggcggcaga cggccgttcg
     1801 gtggtgctgc gcagccgcct gcaacagtcg gccgacggtt atcccaacga tttggatttg
     1861 gatatttcct accgcttgga cgaggacaac cggcttaccg ttagctatcg cgccaccgcg
     1921 ctcggcgaca cggtgttcga cccgacgctg cacatttact ggcggctgga cgcgggcctg
     1981 cacgatgcgg ttctgcatat tccgcagggc ggacatattc cggccgatgc cgaaaaactg
     2041 cccgtctcaa cggtttcaga cgacctcgaa gtatttgatt tcagccggcc caagccgctg
     2101 gatgccgccg ttgccgccct gcgccgcgaa acgggtcggg ccggttttga cgacgcttac
     2161 cgcgtgccgt ccgatatagg ccgtcccgcc gctgtgttgc aagccggacg ccgccgtcgt
     2221 atcagcatat acagcgaccg caacggcttg gtcatcttta ccgccgcccc gcaggatttc
     2281 gcgcggcacg atgcgggcgt ttacgacgcg ctggcgaccg aggcgcagac gctgcccgac
     2341 agcctgaatt ggcccgagtt cggcaatatt cgtctgaaca agggtgatac cagggaggcg
     2401 acgattgctt acggcatcga atccctttct taggagcttc ctaacaccgg ttgcagacgg
     2461 cctttttata gtggattaac aaaaaccggt acggcgttgc ctcggcttag ctcaaagaga
     2521 acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactattt gtactgtttg
     2581 cggcttcgtc gccttgtcct gatttttgtt aatccactat aagatttcac cattccctca
     2641 aatcaatcca aacaggagct tcataaatgt acacaagaat tatggaaatc agcccttgga
     2701 cgctgcgttc ggcaaagctg gaaaaagaac acaaacggct gcaagagagc ctgaccagtt
     2761 tgggcaacgg ctatatgggt atgcgcggca gctttgagga aacctactcc gccgacagcc
     2821 acttgggcac ctacatcgcc ggcgtgtggt tccccgacaa aacccgcgtc ggctggtgga
     2881 aaaacggcta tcccaaatat ttcggcaaag ccatcaacgc gctcaatttc agcaaagtca
     2941 aaatctttgt cgatggtcaa gaggtggact tggcgaaaaa cgatgttgcc ggcttctccg
     3001 tcgaactcga tatgcagcac ggcgtgttgc gccgctcgtt caccgtattc ggcgtgcgtt
     3061 tcgatgtgtg caaattcctg tccgtcgcac aaaaagagct ggctctcatc cgctgggaag
     3121 ccgtatctat cgacggcaaa acccaccaag tccgcatcga ttccatcatc gatgccgacg
     3181 tgaaaaacga agattccaac tacgaagaaa aattctggca agtattggac aaaggcgttt
     3241 cagacgaccg ctcctacatt gccacccaaa ccgtcaccaa tcccttcggc gtggaacaat
     3301 ttatcgtcaa cgccgagcaa actttcgccg gcagcttcaa agccctcggc ggcagccaaa
     3361 ccgactggca ggtctccaat tctttcgagg ccgaagtcgg cggcacgccc aaaacctttg
     3421 aaaaacgggt tattgttacc accagccgcg attatcaggg cttggaagca gtgaaagccg
     3481 caggccgtgc cttgtcggaa aaagtcgcag gcgttgcgtt tgaaaccttg ctggacgcgc
     3541 acaaagcagg ctggctgcac cgttgggaaa tcgccgacgt ggtcatcgaa ggcagcgacg
     3601 aagcgcagca aggcatccgt ttcaatctgt tccaactgtt ctccacctac tacggcgaag
     3661 atgcgcgcct gaacatcggc cccaaaggct ttaccggcga aaaatacggc ggcgcgacct
     3721 attgggatac cgaagcctat gccgtaccgc tctacctcgc actggcggaa cccgaagtta
     3781 cccgcaacct gctgcaatac cgccgcaacc aactgccgca ggcgcagcac aacgcgcgcg
     3841 aacagggctt ggcgggcgca ctctatccga tggtaacgtt tacgggtatc gagtgccaca
     3901 acgaatggga aatcaccttc gaggaaatcc accgcaacgg cgcgattcct tacgccatct
     3961 acaactacac caactacacc ggcgacgagg gctatcttgc caaagaaggc ttggaagtct
     4021 tggtcgaagt gtcccgcttc tgggcggacc gcgtccactt ctccaaacgc aacggcaaat
     4081 acatgattca cggcgtaacc ggcccgaacg aatacgaaaa caacatcaac aacaactggt
     4141 acaccaacac cctcgccgca tgggtattgg actacacccg cgaagccttg gcgaaatacc
     4201 cgcgtccgga tttgaacgtg cgtgccgccg agttggaaaa atgggcggac atcagcgcga
     4261 atatgtaccg tccgcatgac gaagaactcg gcgtattcgt gcaacacgac ggcttcctcg
     4321 acaaagacat ccgccccgtg tccgcgcttt cgcccgacga tttgccgctc aaccagaaat
     4381 ggtcgtggga caaaatcctg cgttcgccct ttatcaaaca ggcggacgta ttgcaaggca
     4441 tctatttctt cggcgaccgt ttcaatatcg acgaaaaacg ccgcaatttc gacttctacg
     4501 aaccgatgac cgtgcacgaa agctcgttgt cgccgtgtat ccactctatt ctcgccgccg
     4561 aactgggcaa agaagaaaaa gccgtggaaa tgtaccggcg caccgcacgc ctggacttgg
     4621 acaactacaa caacgacacc gaagacggcc tgcacattac ttctatgacc ggctcgtggc
     4681 tcgccatcgt ccaaggtttc gcccaaatga aaacttgggg cggtaaactc agcttcgcac
     4741 cgttcctgcc gagcgcatgg acaggctacg ccttccatat caactaccga ggccgtctga
     4801 ttaaagtcgc cgtcggcaaa gaaaacgtcg tcttcaccct gctcaaaggc gagccgctcg
     4861 atttgcaggt gtacggcaaa gacatcacac tcaacggcag ccataccgcc gcgttggaaa
     4921 aataaggagg gcgcaaaatg acgtttaccg cagtgctctt cgatctcgac ggcgtcatca
     4981 ccgacaccgc cgaataccac taccgcgcgt ggaaaaaact cgccgaagaa ctgggcatca
     5041 gcatcgaccg caagtttaac gagcagctca aaggcgtgtc gcgcgacgat tcgctcaaac
     5101 gcatcctcgc gcacggcggc aaaaccgtca gcgaagccga gttcgccgaa ctgacccgcc
     5161 gtaaaaacga caactacgtc gagatgattc aggcagtcaa acccgaagac gtgtatcccg
     5221 gcattttgcc cctgctggaa acattgaaag caaacggcaa aaaaatcgcc ctcgcgtccg
     5281 ccagtaaaaa cggcccgttc ctgctcgaac gcatggggct gacccacttc ttcgacaccg
     5341 tcgccgaccc tgccgccgtc gcgcattcca aacccgcccc cgacatcttc ctcgccgccg
     5401 ccgagggcgt gggcgcggac atccgccgct gcatcggcat tgaagacgct gccgcaggtg
     5461 tcgccgccat caaagcctcc ggcgccttac ccatcggtgt gggcaaagcc gaagacttgg
     5521 gtagcgacat cccgctggtg tccggcaccg ccgagctgac ctacgcctac ctgcaaaacg
     5581 tgtgggcaca gtcgggcagg taaaacgcgt cagataaagt gttaaggaag taaaaggccg
     5641 tctgaacagt gtttcagacg gcctgagacc tttgcaaaat tcctgtccct cccgacagcc
     5701 gaaatccaaa cacaggattt cggctgtttt cgtaccaaat acctcctaat tttacccaaa
     5761 tatcccctta atcctgcccg gatacccgat aatcaggcat ccgggctgcc ttttaggcgg
     5821 cagcgggcgc acttaacctg ttggccgctt tcaacaggtt caaacacatc gctttcaggt
     5881 ggctttgcgc actcacttta atcagtccga aataggttga ccgcgcatag cggaatttac
     5941 ggtgcagcgt accgaagctc
//
LOCUS       DAWEWP010000119         5945 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWEWP010000119 DAWEWP010000000
VERSION     DAWEWP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.119"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(79..483)
                     /locus_tag="WGN53_001595"
                     /pseudo
     CDS             complement(79..483)
                     /locus_tag="WGN53_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(789..2048)
                     /gene="rho"
                     /locus_tag="WGN53_001596"
     CDS             complement(789..2048)
                     /gene="rho"
                     /locus_tag="WGN53_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ0140569.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTSNYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(2268..4652)
                     /gene="ppsA"
                     /locus_tag="WGN53_001597"
     CDS             complement(2268..4652)
                     /gene="ppsA"
                     /locus_tag="WGN53_001597"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ0140570.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYSGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            5064..5885
                     /locus_tag="WGN53_001598"
     CDS             5064..5885
                     /locus_tag="WGN53_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ0140571.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1279 a         1376 c         1691 g         1599 t
ORIGIN      
        1 tctttgagct aaggcgaggc accgccgtac tggtttttgt taatccacta taaaaattta
       61 tggggctgtc ctagataact aggataaact cgattttact aattgtttta aaatggaaat
      121 ttgaactttt atctcactgt tgttaaaacg tcgttcgtac ccctttaaat acagctcaaa
      181 atgcgctttg ggaatgccgt caaacttgcg taaatgacgt tttgcccggt ttcaaaagtt
      241 cccaattcca ttgatatggt tttgtcgttc agcaaaataa ctttcatctg cttctacttt
      301 gccgtcaaac atttccaaat gcggactgtt ttgataaata agtaatcgta aacgatgaaa
      361 ataataggct gcggtatttt tattaacgcc tactaactct gctgctgttc ttgcagttac
      421 acctgcgaca aacagttcaa tgagtttatt ttgtttatac cggcttagac gactttttct
      481 cataggggca actctaactt aatttgaatt tccctagtta tctaggacag ccccaaattt
      541 atacaaaaat gagcgcggtt cggcgcaacc ttgaatcaag ttcccgcatc ggttttcatt
      601 gccggtacgg atgcgttcaa gccggttttg caaaggccgc gctttcggca agcggacacg
      661 gacactgccg acggttgcgc cgttaacggg gggagggagg agctgcgccg accgtgtgaa
      721 tgaaagtgcc gtctgaaacc cgattttcag gcttcagacg gcatttcgca ttaatgcggg
      781 cggcgcgttt atttgccgcg catcagttca aagaaatcat cattgttttt agaggctttg
      841 atttttccga ttaaaaattc ggctgcctcg atttcgtcca tcgggtgcag gaacttgcgt
      901 aagagccaca tacgttgcaa ctggtcgttc ggcacaagca gctcttcgcg gcgcgtgccg
      961 gatttgttga tgttgatggc ggggaagagg cgtttttccg ccatacggcg gtcaaggtgc
     1021 aattccatat tgccggtgcc tttgaattct tcgtaaatca catcgtccat acggctgccg
     1081 gtttcaacca atgcggtggc gatgatggtc agcgaaccgc cttcttccac gttgcgcgcc
     1141 gcgccgaaga aacgtttggg gcggtgcagt gcgttggcat cgacaccgcc ggttaggatt
     1201 ttgcccgagg taggcacgac ggtattgtag gcgcgggcaa ggcgggtaat cgaatccagc
     1261 aggatgacca cgtctttttt gtgttctacc atacgcttgg ctttttcaag caccatttcg
     1321 gcaacttgga cgtggcgttg cgccggttcg tcaaaggtgg aggaaaccac ttcgccgcgg
     1381 acggaacggc tcatttcggt tacttcttcg ggacgctcgt caatcaagag gacgatgagt
     1441 tcgacttcgg gatagtttga ggtaacggcg tgggcaatgt tttgcagcat cacggtttta
     1501 ccgcttttgg gcggggcaac caagagggcg cgctgacctt tgccgatagg ggaaatcagg
     1561 tcgatggcac gtccggtcag gttttcttcg gactttaagt cgcgttccag cttcaactgt
     1621 tcggtcggaa acagcggggt caggttttca aacaggattt tatggcggca tacttccggg
     1681 tggtcgccgt tgatggtatc aagcctgacc agggcaaaat agcgttcgtt gtcttttggg
     1741 acgcgcacgc ttccttcgat ggtgtcgccc gtatgcaggt tgaagcggcg gatttgggtg
     1801 ggcgagacat agatgtcgtc ggggccggca agataggacg tgtccgcgct gcggaggaag
     1861 ccgaagccgt cgggcaggat ttcaagcgtg ccggagcagg tgaaaccctc gccttttttc
     1921 atcatctggc ggacgatggc aaatacgaga tcttgtttgc ggaatcggtt ggcgttttcg
     1981 atgccgtgtt cttccgccaa ttctaagagt ttggaaatgt gcagggtttg taattcggag
     2041 acgtgcataa taatgatgta ttttgaagag gaaaaagaca ggcagatgcc gtctgaaaga
     2101 agaagctgac cgttgccggt tgctcgggga agggggaatt gtaggcagtc ggcgcgtggg
     2161 tgtcaaatat tatcgcggac ggggcatcgg caggaaatgc cgtctgagcg gagctgcttg
     2221 gaaaaaaata cccccgcgct tttcaggctc gggggtatgg gcattgatta tttgttcaat
     2281 tcattcgcca aatatagcca agtttcgatg acggtatccg ggttcaggga aacgctttca
     2341 atgccttcct caaccagcca tttggcgaag tccggatggt cggacgggcc ttgaccgcag
     2401 atgccgacat atttgttctg cttgcggcag gcggagatgg caaggtgcag catcactttg
     2461 acggcagggt tgcgttcgtc aaacgattcg gataccaagc cgctgtcgcg gtcgagaccg
     2521 agggtcagtt gggtcatgtc gttcgagccg atggagaagc cgtcgaagta ttgcaggaat
     2581 tgttccgcca ataccgcgtt gctcggcagc tcgcacatca taatcaggcg caggccgttt
     2641 ttgccgcgtt ccaagccgtt ttctttcagg gctttgacaa cggcttcggc ttcgcccaaa
     2701 gtgcggacga acggaatcat gatttcaacg ttggtcaacc ccatttcatc gcggacgcgt
     2761 ttcaaggctt tgcattccaa ggcgaaacag tctttgaagt tgtcggcgac ataacgcgcc
     2821 gcaccacgga agcccaacat cgggttttct tcatgcggtt cgtatacgtt gccgccgacc
     2881 aggttggcgt attcgttgga tttgaagtcg gacatacgga cgatggtttt acgcggataa
     2941 accgatgcgg ccaatgtcgc cacgccttcg gcgattttat cgacgtagaa gtcgacaggg
     3001 gacgcgtaac cggcgatacg gcgggtaatt tccgctttta attcgtcgtc ttgtttgtca
     3061 aattccaaca aggctttggg gtggataccg atttggcggt tgatgataaa ttccatacgc
     3121 gccaagccga tgccttcgct gggcaggttg gcgaagctga atgcgagttc gggattgccg
     3181 acgttcatca tgacttttac aggtgcttta ggcatgttgt ccaaagcaac atcggtaatt
     3241 tgtacgtcca gcaggccgga gtagatgaag ccggtatcgc cttcggcaca ggatacggta
     3301 acttcttgac cgtttttcag caattcggtt gcattgccgc agccgacaac ggcaggaata
     3361 cccagttcgc gcgcgatgat ggcggcgtgg caggtacgtc cgcccctgtt ggtcacgatg
     3421 gcggaagcgc gtttcatcac cggttcccaa tctgggtcgg tcatgtcggt aaccagtacg
     3481 tcgccggctt cgacggaatc catctcggaa gcatctttaa tcaggcgtac cttgccctga
     3541 ccgactttct gaccgatggc gcggccttcg cacaagacgg ttttttcgcc gttgatagaa
     3601 aagcggcgca ggttgcggct gccttcttcc tgggatttga cggtttcggg acgggcttgc
     3661 aggatgtaga gtttgccgtc caagccgtcg cgtccccatt cgatgtccat cgggcggccg
     3721 tagtgttttt cgatggtcag tgcgtaatgc gccaactcgg tgatttcttc gtcggtaatg
     3781 gagaagcggt tgcggtcttc ttcggggaca tcgacgttgg ttaccgattt gccggcttct
     3841 gctttgtcgg taaaaatcat tttgatgtgt tttgagccca tggttttgcg caggatggca
     3901 ggtttgcctg ctttcagcgt gggtttgaac acatagaatt cgtcgggatt gactgcgcct
     3961 tgtacgacgt tttcgcccag accgtaggat gaagtgacaa agacgacttg gtcgtaaccg
     4021 gattcggtat cgagggtgaa catcacgccg gacgcgccgc tgtcggaacg caccatacgc
     4081 tgcacgccgg cggaaagggc gacgatgtcg tgttcgaagc ctttgtggac gcggtaagaa
     4141 atggcgcggt cgttgtacag ggaggcgaat acatggcgca tcgcttcttt aacgttttcc
     4201 aagccgttga tattcaggaa ggtttcctgt tggccggcaa atgatgcgtc gggcaggtct
     4261 tcggcggttg cggaagagcg tacggcaacg gaaatgtccg cgccgccggc atcggcaacc
     4321 attttgttcc atgccgcttc gatttcggca tcgagctgtt cggggaaggg cgtgtccaaa
     4381 atccattggc ggatttcttt gccgacgcgt gccagttcgg caacgtcttc gacatctaat
     4441 tttgccagtg cggcggaaat gcgttcgctc agaccgttgt gtgcgaggaa tgcgcggtag
     4501 gcatcggctg tggtggcaaa gccgccgggg acgcgaacgc ctttttcggt cagctgactg
     4561 atcatttcgc ccagcgaggc gtttttaccg cccacgcgtt caacatctgt catacgcagg
     4621 ttttcaaacc aaattacgta gttgtcggcc atttgtgtgt ccaatccaaa atatgttaaa
     4681 aaagaaacaa atccgcgtgc ttattttaag cgattcgttc ctctgctgtc atgtgtttta
     4741 tccgttttaa aatcatgatg ccgtctgaaa aattgcggtt tcggcgtgtg tagcggtttg
     4801 aaacttgcag ccggtatact tctttttttg ggtattttct ttgtaaaaca ggtggtttga
     4861 ataggttaat gttttttctg tttgattttt ttgtttattt tttaaaattt tctgccaaaa
     4921 aatactttat ataaataatt ttatttcaaa attatattgt gtctgtttgg gtgtaatccg
     4981 aggtaggtgt gctgcgagtg ctttccttgt gtctgccgct gctgttatga tgggatttta
     5041 aacctgtgtt ttaaggatgg aagatgagca gtccgcgcca tgtgttttac atttccgacc
     5101 gtaccggtct gactgccgag aatatcggcg aggctttgct gaaccagttt ggcaatctgt
     5161 cgttcaaacg ccatacgcat ccgtttgtcg atacgccgga aaaggcgcgc gcggtggtgg
     5221 agaaggtcaa tcggagccgg caggaaaacg gtcagcgtcc gattgcgttt gtcagtgtgg
     5281 ttgatgacga aatccgtcgg attatcaaag gggcggatgc ttttcagatt aatttctttg
     5341 agactttttt gggactgttg gagaaagaac tcaataccga agccacggca tccgagcagg
     5401 ggcatcacag tatcggtaat acgaagcgtt acgatgcgcg tatggaggcg gtgaatttct
     5461 cattgaacca cgatgacggg gtcagcgata agaaccttca ggaagcggat gtaatcttga
     5521 tgggtgtatc gcgttcgggc aaaacgccga cctgccttta cctcgccctg caatacggca
     5581 tccgtgcggc aaactatccg ctgattcccg acgatttgga atcggccgat ctgccgcgta
     5641 tggtcaagcc ttatagggat aagctgttcg ggttgaccat ccagccggaa cgtttgcagg
     5701 ccatccgcca agagcgccgc ccgaattcaa cttatgccaa aatcgataca tgccgcagcg
     5761 aggtggcgga cgcgcagagt atgttcagac ggcatgggat tccgtttgcg aatacgacgg
     5821 ataaatcagt ggaagagctt gccgtacaca tccttcaggc gtgcaagctc aaacgcaggt
     5881 tttgacgggt tttgattcgg tttgaaggtg gaactgccgt ctgaaatcag gtttcagacg
     5941 gcagt
//
LOCUS       DAWEWP010000120         5929 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWEWP010000120 DAWEWP010000000
VERSION     DAWEWP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.120"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            55..1593
                     /gene="murJ"
                     /locus_tag="WGN53_001599"
     CDS             55..1593
                     /gene="murJ"
                     /locus_tag="WGN53_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ0140572.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            1726..2682
                     /locus_tag="WGN53_001600"
     CDS             1726..2682
                     /locus_tag="WGN53_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEZ0140573.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSGSHYAVELMLAVRDRGWNGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGERGENALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARSLHGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHAGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            2733..3515
                     /gene="trpC"
                     /locus_tag="WGN53_001601"
     CDS             2733..3515
                     /gene="trpC"
                     /locus_tag="WGN53_001601"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ0140574.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADNIEAEVGKLF"
     gene            3581..5881
                     /gene="recQ"
                     /locus_tag="WGN53_001602"
     CDS             3581..5881
                     /gene="recQ"
                     /locus_tag="WGN53_001602"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ0140575.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFISSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMAGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGETEVMLRPLK
                     RGKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         1293 a         1762 c         1618 g         1256 t
ORIGIN      
        1 aaagtctgat cctgccgccc taaagggcgg ggtttcaacc gaaaaggaaa tacgatgaat
       61 atgctgggag ctttggcaaa agtcggcagc ctgacgatgg tgtcgcgcgt tttgggattt
      121 gtgcgcgata cggtcattgc gcgcgcattc ggcgcgggca tggcgacgga tgcgtttttt
      181 gtcgcgttca aactgcccaa cctgcttcgc cgcgtgtttg cggagggggc gtttgcccaa
      241 gcgtttgtgc cgattttggc ggaatataag gaaacgcgtt ctaaagaggc gacggaggct
      301 tttatccgcc atgtggcggg gatgctgtcg tttgtgctgg tcatcgttac cgcgctgggc
      361 atacttgccg cgccttgggt gatttatgtt tccgcacccg gttttgccaa agatgccgac
      421 aaatttcagc tctccatcga tttgctgcgg attacgttcc cctatatctt attgatttca
      481 ctttcctctt ttgtcggctc ggtactcaat tcctatcata aattcagcat tccggcgttt
      541 acgcccacgt ttctgaacgt gtcgtttatc gtattcgcgc tgtttttcgt gccatatttc
      601 gatcctcccg ttaccgcact ggcatgggcg gtttttgtcg gcggcatttt acaactcggc
      661 ttccaactgc cctggctggc gaaactgggt tttttgaaac tgcccaaact gaatttcaaa
      721 gatgcggcgg tcaaccgcgt catgaaacaa atggcacctg cgattttggg cgtgagcgtg
      781 gcgcagattt ctttggtgat caacacgatt ttcgcgtctt atctgcaatc gggcagcgtt
      841 tcatggatgt attacgccga ccgcatgatg gagctgccca gcggcgtgct gggggcggca
      901 ctcggtacga ttttgctgcc gactttgtcc aaacactcgg caaaccaaga tacggaacag
      961 ttttccgccc tgctcgactg gggtttgcgc ctgtgtatgc tgctgacgct gccggcggcg
     1021 gtcggactgg cggtgttgtc gttcccgctg gtggcaacct tgttcatgta ccgcgaattc
     1081 acgctgtttg acgcgcagat gacgcaacat gcgctgattg cctattcttt cggtttaatc
     1141 ggtttgatca tgattaaagt gttggcatcc ggcttttatg cgcggcaaaa catcaaaacg
     1201 cccgtcaaaa tcgccatctt cacgctcatc tgcacgcagt tgatgaacct tgcctttatc
     1261 ggcccactga aacacgtcgg actttcgctt gccatcggtc tgggcgcgtg tatcaatgcc
     1321 ggattgttgt tttacctgtt gcgcagacac ggtatttacc aacctggcaa gggttgggcg
     1381 gcgttcttag caaaaatgct gctctcgctc gccgtaatgg gcggcggact gtgggcggcg
     1441 caggcttgcc tgccgtttga atgggtacgc gccggcggaa tgcggaaagc ggggcagctc
     1501 tgcatcctga ttgccgtcgg cggcggactg tatttcgcat ctctggcggc tttgggcttc
     1561 cgtccgcgcc atttcaaacg ggtggaaagc tgaccgatgc cgatatcctt tttcggaaaa
     1621 cgctttttcc gcgcctatat tcgggcgcgc atcccccacg tccgcaccag ccggacgggc
     1681 tgccgcatca tcaatgccgt ctgaaaacaa gaaaaccgat accttatgat tttaacaccg
     1741 ccggacacgc cctttttcct ccgcaacggc aatgccgaca cgattgccgc caaattcctg
     1801 caacgccccg cacctgcata ccgccgcgag ctgcttccag acagcacggg taaaaccaaa
     1861 gtcgcctacg acttttcaga cggcatttcg cccgacgcac cgctggtcgt gctgtttcac
     1921 ggtttggaag gaagcagtgg cagccattac gcggtcgaac tgatgctcgc ggtacgcgat
     1981 cgcggttgga acggcgcagt cgtccatttc cgcagctgcg gcggcattgc caacaccgct
     2041 ccggtgttct accacttggg cgataccgcc gaaatcgcct ttactttgga cacgcttgcc
     2101 gcgcgttacc gtgaaatata cgccgtcggc gtatcgctgg gcggcaacgc gctggcaaaa
     2161 tatttgggcg aacggggcga aaacgcgctg ccgcaagccg ctgccgtcat ctccgcaccc
     2221 gtcgatgcag aggcggcagg cagacgcttc gacagcggca tcacgcggct gctctacacg
     2281 cgctacttcc tccgcaccct gatacccaaa gcacggtcgc tccacggttt tcagacggca
     2341 tttgccgcag ggtgcaaaac actgggcgag tttgacgacc gcttcaccgc accgctgcac
     2401 ggctttgccg accggcacga ctactaccgc caaacttcct gcaaaccgct gctcaaacac
     2461 gttgccaaac cgctgctcct gctcaatgcc gccaacgacc ccttcctgcc gcccgaagcg
     2521 ctgccgacac cggacgaagt gtccgaagcc gttaccctgt tccagccggc acacggcgga
     2581 cacgccggct ttgtcggcag caccggcggc aggctgcacc tgcaatggct gccgcagacc
     2641 gtcctgtcct atttcgacag cttccgcaca aacaggcggt aacggtttga tgctaatatt
     2701 ccccctttcc ccggacaaat acggaacacg acatgaccga catcctcaat aaaatcctta
     2761 ccgccaaggc acaggaagtt gccgctcaaa aagccgccgt caacgccgaa cacatccgcg
     2821 cacttgccgc agaagccgcg cccgtccgca gcttcatcgg ttcgatacgc ggcaaacacc
     2881 gcctaaacct gcccgccgtc attgccgaaa tcaaaaaggc aagcccgagc aaaggcctga
     2941 tccgtccgga cttccgaccg gccgagattg cacgcgccta tgaaaacgcc ggggcggcgt
     3001 gtttgtccgt actgaccgac gaaccctatt tccaaggttc gcccgaatac ctcaaacagg
     3061 cgcgcgaagc cgtatcgctg cccgtgctgc gcaaagactt catcatcgac gaatatcagg
     3121 tttatcaggc gcgcgcatgg ggggcggatg ccgtcctgct gattgccgca gcactggaac
     3181 aggaacaatt ggaacgcttt gaagcggtgg cgcacgaatt gggcatgacc gtcctgctcg
     3241 agctgcacga tgaaagcgaa ttggaaaaat gccgcaacct gaccacgccg ttgtggggtg
     3301 taaacaaccg caacctgagg acttttgaag tctccctcga ccaaaccctg tcgctgctgc
     3361 ccgcgctgga aggcaaaacc gtcgttaccg aaagcggcat tacaggcaag gcggatgtgg
     3421 aatttatgca atcgcgcggc gtgcatacct tcctgatcgg cgaaacgttt atgcgtgccg
     3481 acaatattga ggcggaagta ggcaaactct tctaaatccc gatttcagac ggcattccct
     3541 ttctttaacc gacacaccct ttaccggaca cggaaccatc atgcaccgac cgaccgccaa
     3601 acagattctg cacgaagtat ttggttatcc cgaatttcgc ggcaggcagg aggctgtcat
     3661 caatactttg gcaggcggcg ggagtttgac ggtgctgatg ccgacgggcg ggggcaagtc
     3721 tttgtgttac cagattcccg cgctgatgcg cgaaggcgtg gcggttgtcg tatcgccgct
     3781 gattgcgctg atgaacgacc aagtggccag cctgcatgtg gccgggattg aagcagcggc
     3841 agtcaacagc ggcacatcgg cagatgaggc gcgcgagatt gccgacaagc ttgcccaagg
     3901 ccgtctgaaa ctgctttatg tcgcgccgga acgcttggtt accgatcgct ttttgcgttt
     3961 tctcgaccaa caaaccgtca gcctgtttgc cattgatgag gcgcattgcg tcagccagtg
     4021 gggacacgat ttccgccccg aataccaaca gctcggcatg cttgccgaac gctatccgaa
     4081 cgtcccgcgc atcgccctga ccgccaccgc cgatgccgcc acgcgcgccg acatcaagca
     4141 ttatctgcac ttggacgatg cgcccgaatt tatttccagc ttcgaccgcc cgaatattta
     4201 ttatcaggtt atcgaaaaaa acaacggcaa aaaacaactg cttgaattta tccgcaaaga
     4261 aatggcgggg caaagcggca ttgtgtattg cctaagccgc aaaaaggttg aagatgtggc
     4321 gcagtttttg cgtgaaaacg gattaaacgc gattccgtat catgctggtt tgagcatgga
     4381 cgtgcgcgag gaaaaccaac gccgctttac gcatgaagac aatattatcg tggtggcgac
     4441 cgtggcgttc ggcatgggca tagacaaacc cgacgtgcgc tttgtcgccc atctcgatat
     4501 gccccagagt gtcgaacatt tctatcagga atcggggcgt gccggcaggg acgggctgcc
     4561 tgccgcgagc tggctgtgtt acggcttgaa cgattgggtg ttgctgcgcg aacggattgc
     4621 cgaaggcaac agcgatgagg tgcaaaagca aatcgaaatg caaaaactcg atgccatgct
     4681 ttccgtctgc gaaaccgccg cctgccgccg cgtactgttg ctcaaacatt ttggcgaaga
     4741 atccgaaccc tgcggccatt gcgacaactg cctgcatccg cctgtgcggt ttgacggcac
     4801 ggtgttggta caaaaattac tcagctgcgt gtaccgcgcc ggacaacgtt ttgccgccgg
     4861 ttacatcacc aaccttttgc gcggtaaaag cgacgattgg atacgcggca accggcacga
     4921 acaactgtct accttcggca tcggtgcgga gttgtccgac aaagaatggc gcagcgtcat
     4981 ccgccagtgc atcagcctcg gctacctcac cgtcaacatt gcccgatatc aggcattgca
     5041 actgaccgaa gccgccaaaa aagtcctcaa aggcgaaacc gaagtgatgc tgcgtccgct
     5101 caagcgcggc aagcccgccg cccgcaccct caaagacaac tggctgcgta ccgaacgcga
     5161 agaacgcctg tggcaggcat tgcgcgtttg gcgtatgaaa caggcagaag ccgaaggcat
     5221 ccccgcctat atgattttcg gcgacaaaac cctgcgcgac cttgtcgaaa aaatgccgca
     5281 aaacctcaac gggctgcacg acatctacgg cttgggcgaa gccaaaaccg aacgtttcgg
     5341 acacggcata ctcgaagtct gccgcaacgc cgccggcttc agccgcgacg cggtcatccg
     5401 tccgcaaacc gaacgcgaac aacaactgcg tcaaaaactc gaggcctggc ggtatgaaca
     5461 ggcaagggcg gaaaactgcg ccctgcatgc cgtcctctcc gacgaaagcc ttgccgatat
     5521 gcttgccgat acgcccgaaa ccgaaaccga cctcgaaggc gtacacggct tgggcagcgt
     5581 acgcgccgcc aaatacggac gggacatcct cgccgtctgc cgtccgtttt cagacggcat
     5641 cgatgaaacc gccaaacaca aacgccgcct gatgcgcgcc ctgatccaat ggtgcaacga
     5701 aacggcaaaa cacgaacagt ccgaacccta ccgcattctc agcaaagccg ccctacgcgc
     5761 cattgccgcc aaacagccgg aaggtttggc ggagcttgcc gccgtatatg gcgtaggcga
     5821 agaaaaagcc gcacgttacg gtgcggcggt gttggcggta ttggaacgaa atgccgtctg
     5881 aaaaccgtta ccaagtttca gacggcattg cctctattta aaaattcct
//
LOCUS       DAWEWP010000121         5928 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWEWP010000121 DAWEWP010000000
VERSION     DAWEWP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.121"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            309..1256
                     /locus_tag="WGN53_001603"
     CDS             309..1256
                     /locus_tag="WGN53_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0140576.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVALHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1408..2532)
                     /gene="rluD"
                     /locus_tag="WGN53_001604"
     CDS             complement(1408..2532)
                     /gene="rluD"
                     /locus_tag="WGN53_001604"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ0140577.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTLWIKEGAVIVNDKPSQPKDKMIGGEQIRVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWRGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCSDPVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2531..3334
                     /locus_tag="WGN53_001605"
     CDS             2531..3334
                     /locus_tag="WGN53_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ0140578.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3392..5611)
                     /locus_tag="WGN53_001606"
     CDS             complement(3392..5611)
                     /locus_tag="WGN53_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ0140579.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTAVRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRIRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1435 a         1869 c         1427 g         1197 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat atgtttcaac aggggatggc
       61 acatcaagca ccgcactgcg tgttgcccga acgagactac cctccgatta gattctatcg
      121 ctataaacag acgggtttca accgaaaagg aatggggata aagtccattt ctgacacctc
      181 tcgggcgatg ccgtctgaaa accaatctcc actttcagac ggcattgttt aggcacggtc
      241 attttgcaaa ttaaggttaa ggcgtgtatg cttgccttcc cttaaaaaca aacacgcaac
      301 gcattattat gaatatcctc agtaaaatca gcagctttat cggaaaaaca ttttccctct
      361 gggccgcgct ctttgccgcc gccgcttttt tcgcgcccga caccttcaaa tgggcggggc
      421 cttatattcc ttggctgttg ggcattatta tgttcggtat gggtttgacg ctcaaacctt
      481 ccgacttcga tattttgttc aaacatccca aagccgtcat catcggcgta atcgcacaat
      541 tcgccattat gccggcaacc gcctggctgc tgtccaaact gttgaacctg cctgccgaaa
      601 tcgcggtcgg cgtgattttg gtcggctgct gcccgggcgg tacggcttcc aatgtgatga
      661 cctatctggc gcgtggcaat gtggctttgt cggttgccgt tacgtctgtt tccaccctga
      721 tttccccatt gctgactccc gccatcttcc tgatgcttgc cggtgaaatg ctggaaatcc
      781 aagcggccgg tatgttgatg tccatcgtca aaatggtttt gctccccatt gttttgggtt
      841 tgattgtcca taaggttttg ggcagtaaaa ccgaaaagct gaccgatgcg ctaccgctgg
      901 tttccgttgc cgccatcgtg ctgattatcg gcgcggttgt cggggcaagc aaaggcaaga
      961 ttatggaaag cggcctgctg atttttgccg ttgtcgcact ccacaacggc atcggctacc
     1021 tgctcggctt ctttgccgcc aaatggaccg gcctgcctta tgatgcacaa aaaacgctgg
     1081 ccatcgaagt cggtatgcaa aactcggggc tggccgccgc gcttgccgcc gcacactttg
     1141 ccgctgcgcc ggtcgttgcc gttccgggcg cattgttcag cgtgtggcac aatatctccg
     1201 gctcgctgct ggcaacttat tgggcggcca aagccggtaa acataaaaaa ccctaaacgc
     1261 ctttcatcat aatgccgtct gaacggggca aacgaaaccg gcggtttctg cccgacccct
     1321 tcagacgcct ttgtttgaaa tcggcgggga cgattatccg gctgctccga ttcggctacc
     1381 tgcgttcgcc ccgcctttca agccacctca ctccctcaca taaaccactt cgacatcgta
     1441 gtcgtcttcg ttccaatcgt catcgtcgtc cgcgccgaat ttgtcctgcc attcctcttc
     1501 attgctcaaa gacgaatcca aaccggcttc aagacggagg acggacaaca aatgatatat
     1561 gtcgtccgga atcggtgcct caaacgaaac ggtttcgccg ctttccggat gggtgaaact
     1621 caagcggtag gcgtgcaatg cctgacgcgc gcccaaactt ttaacggctt ctttcaccgg
     1681 gtcgctgcac ggatggcgcg ggttgccgta cacgggatcg ccggcaagcg gatggttggc
     1741 ctcgcgcata tggacgcgga tttggtgcgt cctgcccgtt tcgagcgagc attcgatata
     1801 gctgtgggta agatagcgtt ccaacacttt gacgtgggta acggctggtt tgccgccgaa
     1861 tttgacgact gccattttca ggcggttgtg cggatcgcgt ccgatttggg tttcgatttt
     1921 accgtcgaag gggacgatgc cgttggcgac ggcgcggtag atgcgtttga ccgtgcgctc
     1981 ttgaagctgc ctcacgaggg aattttgcgc cggcagggtt ttggcaacca ccatcagccc
     2041 gctggtttct ttgtccaaac ggtgtacgat gcctgcacgc ggtacttggc tcaattcggg
     2101 acagtgcgcc aacaggccgt tgagcagcgt ccccctccag ttgcctgccg ccggatgcac
     2161 caccagtccg gccggtttgt tgacgacaat gacggtatcg tcttcgtaaa caatatccaa
     2221 agccataggc tctggaacaa acgccagatt ttcctcactc ggacggacgg ttacacggat
     2281 ttgctcaccg cctatcattt tgtctttggg ttgcgaaggt ttatcgttta caataaccgc
     2341 gccctcttta atccataatg tcaggcggct gcgcgaatag tcgggcagaa gtttcgccaa
     2401 caccgcatcc aaccgcccgc cggcaagctc gagcggaacg gtcagaccga tacggttttc
     2461 agtttcggaa gctgacgcaa agtctgaatc gtcgctataa tcggattcat tatcaaagga
     2521 agtattctgc atgaaaaaaa ttcttttaac ggtttcatta ggtttggcac tgagtgcctg
     2581 tgccactcaa ggtacggtcg ataaagatgc ccagattacc caagattgga gtgtggagaa
     2641 gctctatgcc gaagcccagg acgaattgaa cagcagcaat tatacgcggg ctgtcaagtt
     2701 atacgaaatc ttggaatcgc gcttccccac cagccgccat gcccggcagt cgcaactgga
     2761 taccgcatac gcctattata aagacgatga aaaagacaaa gctctggcgg caatcgaacg
     2821 cttccgccgc cttcacccgc aacacccgaa tatggattac gcgctatacc tgcgcggttt
     2881 ggtactgttc aacgaagatc agtctttctt gaacaagctg gcctcgcaag actggtccga
     2941 ccgcgacccg aaagccaacc gcgaagcgta ccaggcgttt gcggaactcg tccaacgctt
     3001 ccccaacagc aaatacgccg ccgatgcgac cgcacgtatg gtcaaactgg tcgatgcact
     3061 gggcggcaat gaaatgtcgg tggcgcgcta ctacatgaaa cgcggtgcat atatcgccgc
     3121 cgccaaccgc gcccaaaaaa tcatcggcag ctaccagaat acgcgttatg tcgaagaatc
     3181 gctcgccata ttggaacttg cctaccaaaa actcggcaaa ccacagcttg ccgccgatac
     3241 gcgccgcgtg ttggaaacca acttcccgaa aagcccgttt ttgacgcacg cttggcagcc
     3301 cgacgatatg ccctggtggc gttactggca ttaagcccat cttcgtaatg ccgtctgaag
     3361 ccgtttcaga cggcatttta tgtttcaaac ctcattcaaa cggttttttc tgccaataga
     3421 atttataccc tctcaaacgt tgagccttca cgccgccgcg ccccaagccg aattgcagcg
     3481 cgcccgacaa atcggtacgc aacaatttga tgccgtgtgc acggatacgg ttttgcaccg
     3541 cttcggtcgg atgtttgtag gcgttggcat aaccgcttga agcaacggca tattcgggcg
     3601 agacggcatt gaggaacacg cccgacgagg acgtattgct gccgtgatgt cccaaaacca
     3661 aaacctggct gtacaggttg cctccatact tgccgaccag gctttcctcg cccttcgtat
     3721 ccaaatcgcc cgttaccagc agtgccgcac cgcccgccac aacacgcaaa acacaacttt
     3781 tcccattatc atcgatgttt ttgcgttcag acggcatcaa aaactcaaaa tccacgccgt
     3841 cccattgcca acgctgttcc gcacaatgcc gcgccccctc ataaaattcc ggctgcccgg
     3901 cataaatccc gccgttcggt atcttgccga ctgcctgaaa accgccgtcg tggtcgctgt
     3961 cgtgatgcga cagaaccagc ttgtccaaac ggcggacacc cgccgcattc aaactcggca
     4021 caatccccat ctgcgccgcc gctgccgttc cggtgtcaaa caaaagatga tgatttgccg
     4081 tctgaaccga caccgacaaa ccctgccccg catcccaaac cgtaaccgca acctcatttt
     4141 ccggcacgcc gggtgaacgg taaaacacaa accctgccaa cagcaacacc gcccacggac
     4201 gcaaacccaa gccgcgcggc agcagcaaca gcaaagcggc acacaccgcc aacaccaaca
     4261 gcggcaaagg tgcggcggca acggcaaact cgggcgacac atcggcaagc cacaccaaaa
     4321 accgcaaagt atattccgcc aaaaatgccc ccaactgttg caaaggcgca aacggcacga
     4381 ccgaacccag caacgccagc ggcgtcaata cccaagaaaa ccacggaatc gccaccgcat
     4441 tgaccaaagg gctgattaaa ggcagcgaag caaacagata accgagcaaa accagcgaca
     4501 acaccgaagc cgcccactgc ccgcgcacgg cggtttgccg tttcccttcg tgcaaacgcc
     4561 ccgaacacgc ccatatcagg gccgccacca aaccgaaaga cagccaagtc cccacaccca
     4621 agaccgccga cgggtcgaac agcagcaccg ccgccaacgc ctgccaccac gtcgcccacg
     4681 ccgacaatct tcccctgcgc caagcccacg caaacgccgc caacatcaaa acgctgcgct
     4741 gcgtcggcac ggaaaaaccg gcaagcagcg cgtaaaacag cgcgcctgca caccccgccg
     4801 ccaaaaccca cgcacgcggc cgggcaggaa ggcgcgggga acaggcaagc agccgcttcg
     4861 ccagccacgc aaacattacc gccaccatcg taacgtgcaa acccgaaata ctgaccaaat
     4921 gcgtcagccc caacggccgg aacgcctgcc acaattcggg gcgcaatgcc gactgttcgc
     4981 ccacgctcaa cgcgcgcatc agcccgatgc cgtctgaaag cccgccgtcc gcatccgcct
     5041 gctgccaatt acggctgatg cggctgcgcc aaaccgcaat cccccaaccg cttccgccat
     5101 gcagcaaaac cctgtccgca ccgaccgtcc ccgcgccgcc tatcccgttg gataatgccc
     5161 acgcctcacg gttcaaaccg cgcaggttca attcgccgac gacggggtgc acacgtgccg
     5221 ttatccgcca tctgctcccg accgcccatt cgcgccgttt gtagtccgac agcagcaaat
     5281 caaacgtccg accaccgctg tccacagcct ttgccgcaaa ctgcacgcgc cgcccgtccg
     5341 acctcggcat atccgccact tccaccgtca acggcacacc tgaaaccgcc tccgcccgcc
     5401 attgcgaaga cagtgccgct tccgttctga atacgccgta agccgcgccc gccaacacgc
     5461 acagcatcag accggcaaac gcaaaacgcc ttgcaaacac agcaaaaacc gcaaaagccg
     5521 ccagccaaaa cagccaatgc ggcacgaccg gcagcgcaaa cgaagccgcc gcgccgacaa
     5581 cccaacacgg caaaaaccaa cgacggaaca cgtctcccca ccccagatat ttcaaaaata
     5641 aacggataat aaagcaaatc cgaaaaaccg tcataacaag atttgaaaat gatatagtgg
     5701 tttaaattta aatcaggaca aggcgacgaa gccgcagaca gtacaagcag tacgacaagg
     5761 cgaggcaacg ctgtactggt ttttgttaaa ccactataag aatgcaaacg aaaagcctca
     5821 gtattatccg agacaacggc aaactttcca tcgtcattcc cgcacaagcg ggaatccaaa
     5881 cttgtccgca cggaaactta tcggataaaa cggtttctta gattccac
//
LOCUS       DAWEWP010000122         5827 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWEWP010000122 DAWEWP010000000
VERSION     DAWEWP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.122"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1040..3766)
                     /locus_tag="WGN53_001607"
     CDS             complement(1040..3766)
                     /locus_tag="WGN53_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HEZ0140580.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAEQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKSSDTLSKEQVLNIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGVSQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSS
                     EYGNGALAGSVAFQTKTAADIIGEGKQWGIQSKTAYSGKDHALTQSLALAGRSGGAEA
                     LLIYTKRRGREIHAHKDAGKGVQSFNRLVLDEDKKEGGSQYRYFIVEEECHNGYAACK
                     NKLKEDASVKDERKTVSTQDYTGSNRLLANPLEYGSQSWLFRPGWHLDNRHYVGAVLE
                     RTQQTFDTRDMTVPAYFTSEDYVPGSLKGLGKYSGDNKAERLFVQGEGSTLQGIGYGT
                     GVFYDERHTKNRYGVEYVYHNADKDTWADYARLSYDRQGIDLDNRLQQTHCSHDGSDK
                     NCRPDGNKPYSFYKSDRMIYEESRNLFQAVFKKAFDTAKIRHNLSINLGYDRFKSQLS
                     HSDYYLQNAVQAYDLITPKKPPFPNGSKDNPYRVSIGKTTVNTSPICRFGNNTYTDCT
                     PRNIGGNGYYAAVQDNVRLGRWADVGAGIRYDYRSTHSEDKSVSTGTHRNLSWNAGVV
                     LKPFTWMDLTYRASTGFRLPSFAEMYGWRAGESLKTLDLKPEKSFNREAGIVFKGDFG
                     NLEASYFNNAYRDLIAFGYETRTQNGQTSASGDPGYRNAQNARIAGINILGKIDWHGV
                     WGGLPDGLYSTLAYNRIKVKDADIRADRTFVTSYLFDAVQPSRYVLGLGYDHPDGIWG
                     INTMFTYSKAKSVDELLGSQALLNGNANAKKAASRRTRPWYVTDVSGYYNIKKHLTLR
                     AGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
     gene            complement(3853..5652)
                     /locus_tag="WGN53_001608"
     CDS             complement(3853..5652)
                     /locus_tag="WGN53_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0140581.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVETVQDMHSKPK
                     YEDEKSQPESQQDVSENSGAAYGFAVKLPRRNAHFNPKYKEKHKPLGSMDWKKLQRGE
                     PNSFSERDELEKKRGSSELIESKWEDGQSRVVGYTNFTYVRSGYVYLNKNNIDIKNNI
                     VLFGPDGYLYYKGKEPSKELPSEKITYKGTWDYVTDAMEKQRFEGLGSAAGGDKSGAL
                     SALEEGVLRNQAEASSGHTDFGMTSEFEVDFSDKTIKGTLYRNNRITQNNSENKQIKT
                     TRYTIQATLHGNRFKGKALAADKGATNGSHPFISDSDSLEGGFYGPKGEELAGKFLSN
                     DNKVAAVFGAKQKDKKDGENAAGPATETVIDAYRITGEEFKKEQIDSFGDVKKLLVDG
                     VELSLLPSEGNKAAFQHEIEQNGVKATVCCSNLDYMSFGKLSKENKDDMFLQGVRTPV
                     SDVAARTEANAKYRGTWYGYIANGTSWSGEASNQEGGNRAEFDVDFSTKKISGTLTAK
                     DRTSPAFTITAMIKDNGFSGVAKTGENGFALDPQNTGNSHYTHIEATVSGGFYGKNAI
                     EMGGSFSFPGNAPEGKQEKASVVFGAKRQQLVQ"
BASE COUNT         1377 a         1488 c         1287 g         1675 t
ORIGIN      
        1 gttcccacgg tcaggatttc agattgcgga catctgtcag aaaagacaaa aaaccttccg
       61 ccgtcattcc ctacaggcga gaatccgatc cgttgaaatt cggttgtttt aaataaattc
      121 ttacagcttt gattttctgt ttttccgata acgccgtaac tttgaaacgc gaaagcggta
      181 atccgatccg ttgggatttt gcaacttcaa atcaatccgc aaactgaaat cccgtcattc
      241 ccgcgcagtc gtgaatccga acgcgtccgc acgaaaacct gcatcccgtc attcccacgg
      301 aagtgggaat ctaggacgta aaatctcaag aaaccgtttt atccgataag tttccgcacc
      361 gacagacctg gattcccgcc tgcgtgggaa tgacgaaatt tcagcgagcc gtagggtggg
      421 ctgtagggtg ggcttcagcc caccaatttc accgcatcaa aggtttttgg gaatacggcg
      481 gtttcggttt tcggtgggaa tgggcggaat ggataacatt gacggagttg ggggaatagg
      541 tggaaacggt gggattggtg ggctgaagcc caccctacag ccagccctat gtccacgctg
      601 cacatcctac gcccgccttg cgtttcttat aagcagcatt ttacgcaata ctttgcgtcc
      661 gctgaaaaaa tttcaaacgg tcttttttat caaatgcgaa aaatataacg tcattcccgc
      721 gcaggcggga atctagtctg tcggtgcgga aacttatcgg ataaaacggt ttctttagat
      781 tttacgccct agattcccgc ctgcgcggga atgacgggat ttgaggtttc tgtttttgat
      841 tttccgcctt cgcgggaatg acggtttaga agttacccga aacctcaaaa aaaaccgaaa
      901 ccgaacggtc tggattcccg cctgcgcggg aatgacgggt ttcgagatta cggtgtatcg
      961 gggatgatgg gaaacggtgg gaattgtgta aaaaatgccg tctgaagcct ttcagacggc
     1021 atttgcggcg tttggacgtt taaaacttca tttccaagct aaatgtgtag tttcggccgg
     1081 gggcggcata tcggttgtaa acgccgacat ttttgtgttg gttgactgcg ccgccggcag
     1141 tttgccgcac attttcccaa gtaacatagc ggtagttgag gaggttgtac acacctgcgc
     1201 gcagggtcag gtgtttcttg atattgtaat atccggaaac atccgtaaca taccaaggcc
     1261 gcgtccgccg tgatgctgct tttttagcat tggcattacc gttcaacagc gcctggctgc
     1321 cgagcagttc gtcaacagat tttgccttgg aataagtaaa catcgtattg atgccccata
     1381 ttccgtcagg atggtcgtaa cccaaaccca atacatatcg tgaaggttgg acggcatcaa
     1441 agagatatga agttacaaac gtcctgtcgg cgcgtatatc ggcatctttg accttgatac
     1501 ggttataggc aagcgtggaa tacaacccgt ccggcaaccc gccccatacg ccgtgccaat
     1561 cgattttacc caaaatattg ataccggcta tccgtgcatt ttgggcattt cggtatccgg
     1621 ggtcgccaga agccgaagtt tgcccgtttt gagttcgggt ttcataaccg aatgcaatca
     1681 ggtcgcgata ggcattgttg aaatagctgg cttccaaatt gccgaagtcc cctttaaata
     1741 caatacctgc ctctctatta aaggattttt ccggtttcag atccaacgtt ttcaaagact
     1801 ccccggctct ccagccatac atttcggcaa acgacggcag acggaagccc gtagaagcgc
     1861 gataagtcaa atccatccag gtgaaaggtt tgaggactac gcccgcgttc caagaaaggt
     1921 tgcggtgagt gccggtagag acactcttat cttccgaatg cgtgctgcgg taatcgtaac
     1981 gtatgcctgc tccgacatcc gcccacctgc ccaaacggac attgtcttga acggctgcat
     2041 aataaccgtt gccgccgata ttcctcggtg tgcagtctgt ataggtgtta ttgccgaaac
     2101 ggcatatcgg cgatgtattg accgtggtct tgccgataga caccctatac gggttgtctt
     2161 tgcttccgtt gggaaacgga ggctttttcg gggttatcaa atcatatgcc tgaactgcgt
     2221 tttgaagata ataatcgctg tgggacaatt gcgacttaaa gcggtcgtac cctagattga
     2281 tactcaaatt gtgacggatt ttggccgtat caaatgcctt tttaaatact gcttggaaca
     2341 ggtttcggct ttcttcataa atcatccggt cggatttata gaaagaatac ggtttattgc
     2401 cgtcgggacg gcaattttta tccgaaccgt cgtgagagca atgcgtctgc tgcaaacggt
     2461 tgtccaaatc tataccttgc cggtcataag aaagtcgggc gtaatcggcc caggtatcct
     2521 tatcagcatt atggtaaaca tattcgaccc cgtagcggtt tttagtatgg cgttcatcat
     2581 aaaacacgcc ggtaccgtaa ccgataccct gcaatgtact gccctctccc tgaacaaaca
     2641 gcctttctgc cttattatcg cccgaatatt tgccaagacc tttcagcgaa ccgggtacat
     2701 aatcttcact ggtaaaatag gcaggaacag tcatatcccg tgtatcaaag gtctgctgcg
     2761 tacgttcgag aacggctccg acataatggc ggttgtccaa atgccaaccc ggtcggaaca
     2821 gccatgattg gctgccatac tcaagcgggt tcgcaagtaa gcggttggag ccggtataat
     2881 cctgcgtgct gacggttttg cgctcatctt tgaccgaggc atcttctttc agcttgtttt
     2941 tacaggccgc atatccattg tggcattctt cttcgacaat gaaatatctg tactgactgc
     3001 caccctcctt cttgtcctcg tccaacacca gccggttgaa gctctgcaca cccttgccgg
     3061 catctttatg cgcatggatt tcccgacccc gccgtttagt ataaataagg agggcttccg
     3121 cgccgccgct gcgtccggca agcgcaaggg attgcgtcag ggcatggtct tttcccgaat
     3181 aggcagtttt actctgaatg ccccactgtt ttccctctcc gataatgtcg gctgcggttt
     3241 tggtttgaaa tgcgaccgaa cctgccaatg cgccgtttcc gtattctgat gaattcgaac
     3301 ccttgctgat ttcaacggcc ttgacgtttt catactcgat ttcattgatt gcgccgctgc
     3361 tacccgccgt cctcgtccca cccaatgccg cctgcgcggt gtaggactgt atttgcgaaa
     3421 cgccgtctac cgttaaggaa acgcggtttt tatccatgcc gcgtattgaa tagccggaac
     3481 ttgcgccccg accctgttcg accacggcaa tacccggatc ataacgggtc aggtctcgga
     3541 tattcaaaac ctgttcttta cttagcgtat cggaagactt gaccaacttg cccagcccgg
     3601 ttacttcgtt atcgcggcgg gttttctgtt ttttggcttt tacctgtatg gtatccaact
     3661 gtttttcctg tgcttgttcg gcttgcacat tttctgcata aacgggcagc gcggtcatta
     3721 aagacaggca taaaatattt aatcggaaca aatgttgctg ttgcatagtg tttccctaat
     3781 cttcgctttc agacggcatc ggaaggaacg gtgccgtctg aagccttatt ctcgattgtt
     3841 cggcagccgt gcttattgca caagctgttg gcgtttcgca ccgaatacca ccgatgcttt
     3901 ttcttgtttt ccctctggtg catttcccgg aaatgagaac gatccgccca tctcgatggc
     3961 gtttttgccg tagaaaccgc cggatacagt ggcttcaata tgcgtatagt gggaatttcc
     4021 ggtattttgc ggatccagcg caaagccgtt ttcaccggtt ttcgccacac ctgaaaaacc
     4081 gttgtcctta atcatggcag taatagtaaa cgcaggagac gtacggtctt ttgccgtcag
     4141 tgtgccactg atttttttag tggaaaaatc cacgtcaaac tctgccctat taccaccttc
     4201 ctgattggag gcttcgccgc tccagcttgt gccgttggca atatatccgt accaagtacc
     4261 gcgatatttg gcgtttgcct ccgtccttgc cgccacatcg gatactggag tgcggacacc
     4321 ttgcaggaac atatcgtctt tattttcttt tgacagcttc ccaaaactca tgtaatccaa
     4381 gttggaacaa cacaccgttg ccttcacgcc gttttgctca atctcgtgct gaaatgccgc
     4441 cttattgccc tcagacggca gcagtgaaag ctccactccg tcaaccagca gctttttcac
     4501 atctccaaaa ctgtctattt gctctttctt aaactcctcg ccggtaatac ggtatgcatc
     4561 tatcacggtt tccgttgcag gccctgccgc gttttcccca tccttcttat ctttctgctt
     4621 cgcaccaaac accgctgcaa ctttgttgtc gttgctcaag aatttaccgg caagttcctc
     4681 gcctttcggc ccgtaaaatc cgccttccaa actgtcggag tcggaaataa agggatgact
     4741 tccatttgtt gcacctttat ctgccgccaa cgccttacct ttgaaacggt tgccgtgaag
     4801 agttgcttga atggtgtaac gcgtagtttt tatttgtttg ttttcactat tattttgagt
     4861 aatacggttg ttacgataaa gtgtgccctt tattgtttta tcagaaaaat caacctcaaa
     4921 ctcactagtc ataccaaaat cggtatgacc ggatgatgcc tctgcctgat tacgcaatac
     4981 cccttcttct aatgcagaca acgcccccga tttatctcct cctgctgcac tacccaatcc
     5041 ttcaaacctt tgtttttcca tagcatcagt aacataatcc caagtacctt tataagttat
     5101 cttttccgat ggcagctcct tggaaggttc tttcccttta tagtaaagat atccgtcagg
     5161 tccaaaaaga actatattat tcttaatatc aatattattt ttattaaggt aaacatatcc
     5221 cgaacggaca taagtgaaat ttgtataacc aactacacga ctttgcccat cttcccattt
     5281 tgattcaata agttcagaac taccccgttt tttttccaat tcatccctct cactaaaact
     5341 atttggttct cctctttgca gttttttcca atccattgaa cccaatggtt tgtgcttttc
     5401 cttatattta ggattaaaat gtgcattccg gcgaggtagt tttactgcaa agccataagc
     5461 cgcgccgctg ttttccgata catcctgttg gctttcaggc tggctttttt catcctcata
     5521 cttaggtttg gagtgcatat cttgcacggt ttccacgctg tccaaatcga aactgccgcc
     5581 gccacccaga caagcactca acaaaaacac aggcagcacc atagcagcct gatttaccaa
     5641 tggattgttc atataaaccc aattcaatta aagaatgata aggattatta ttttatttat
     5701 ttttaacaaa tttgcaaata cttttttatt tttttaggga atacaccaaa atcccgtcat
     5761 tcccgcgcag tcgtgaatcc gaacgcgtcc gcacggaaac ctatatcccg tcattcccac
     5821 gtatatc
//
LOCUS       DAWEWP010000123         5822 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWEWP010000123 DAWEWP010000000
VERSION     DAWEWP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.123"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(103..690)
                     /locus_tag="WGN53_001609"
     CDS             complement(103..690)
                     /locus_tag="WGN53_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ0140582.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTSKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            853..2259
                     /gene="dnaB"
                     /locus_tag="WGN53_001610"
     CDS             853..2259
                     /gene="dnaB"
                     /locus_tag="WGN53_001610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ0140583.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMSGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            2414..3091
                     /locus_tag="WGN53_001611"
     CDS             2414..3091
                     /locus_tag="WGN53_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ0140584.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTVNNKCDSGKKGQGMLAFGDKNGNK
                     GYDNDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTTDRVVWTFNQNGTFGYTK
                     DQHLTKQSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRGDRRTR
                     RTMCQYK"
     gene            3115..3726
                     /gene="pilV"
                     /locus_tag="WGN53_001612"
     CDS             3115..3726
                     /gene="pilV"
                     /locus_tag="WGN53_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ0140585.1"
                     /translation="MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMRNHTLSAVDGDFAIDAI
                     KTKAQLAEEQLKRFSYELKNALPDAAAIRYAVCKDSLGAAPTLSAGSTFSPNCDNKAN
                     GDTLIKVLWVNDSAGDSDIARTNLEVSGDNIVYTYQARVGGRE"
     gene            3723..4661
                     /locus_tag="WGN53_001613"
     CDS             3723..4661
                     /locus_tag="WGN53_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ0140586.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHTEKDVVSDVAQKNRL
                     FSLKRNSTNKLIPITESLNIGYPGFTQRLNALIFQYGIDDVNASADTTVVSSCAKIAK
                     PGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGRIAGEEGLFRFQLNDDGQ
                     WGNPQLLVKKINKMDIRYIYVSGCPEDDDAGKEETFRYTDKFNSAQDAVTPAGVEVLL
                     SSGTDTKIAASSDNHIYAYRIDATIRGGNICANRTL"
     gene            4640..5233
                     /locus_tag="WGN53_001614"
     CDS             4640..5233
                     /locus_tag="WGN53_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ0140587.1"
                     /translation="MRKQNALTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRFCPANSTDLCIDKKGMEYKKGTRSVSKPPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            5238..5711
                     /locus_tag="WGN53_001615"
     CDS             5238..5711
                     /locus_tag="WGN53_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ0140588.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSSDVGCEAFSNRKK"
BASE COUNT         1591 a         1285 c         1505 g         1441 t
ORIGIN      
        1 aagtgggaat ctagaaataa aaagcagcag gaatttatcg gaaataactg aaattcaata
       61 ccgcaaaaat ctacccgaaa tgatatagcg gattaacaaa aatcaggaca aggcggcgaa
      121 gcgtttggcg acttcgtccc agttgacgat ttcccagaaa cctttcaggt agttgggacg
      181 gctgttgcgg tagtcgatgt agtaggcgtg ttcccacacg tcgcaggtca gcagcggcgt
      241 gttttcagtg gtcagcggcg tagcggcgtt ggaagtagaa accaaatcca atccgccggc
      301 aggggttttt accagccacg cccaaccgga accaaaagta ccggccgcgc aggcattgaa
      361 cgcttcttgg aatttctcga agctgcccca tttcgcgtcg atggcggcgg ccagttcgcc
      421 ggcaggtttg ccttgacctt tggacgtgaa acccagccag tagaaggtgt ggttccaagt
      481 ttgtgccgcg ttgttgaaca cgccgcccga agattttttc acaatctctt ccaaaggcag
      541 gttttcaaat tcggtgcctt tgatttgatt gttcaggttg gtgatgtagg tttgatggtg
      601 tttgccgtag tggaattcca aagtctcttt gctcagatgc ggggacaatg cgtccagttc
      661 ataaggcagt tgcggcagct tatgttccat tttgtgctcc taatattgtt ttaaatgttg
      721 tattttggca gtgttgctgc aaatagcttg gcagcgcaca tattttacct gttttgaggg
      781 atggaaacca attaaacctg ctttacgcta taatagaaga ttgcaatttc ggcacgacag
      841 ataggatgta ccatgaacga ttacgcagcc atgccgtctg aagaccgtga ggtcggcgca
      901 ttgtcgctgc ctccacattc gatggaggcg gaacaatccg ttttgggtgg gttgatgctg
      961 gaaaatccgg cttgggacag gattgccgat gtggtttccg gtgaagactt ctaccgccac
     1021 gaacaccgcc tgattttccg atccattgcc aaattaatca atgagagccg tcccgcagat
     1081 gtgattacgg ttcaggaaga tttgcagcgg aacgaagaat tggaagcggc aggcggattt
     1141 gaatatctga ttacgctggc gcaaaacacc ccgtctgccg ccaacatccg ccgctatgcc
     1201 gaaatcgtgc gcgagcgttc catcatgcgc cagctcgccg aagtggggac ggaaatcgca
     1261 cgcagcgcat acaatccgca aggcagggac gcggggcagc ttttggacga ggcggaaaac
     1321 aaagtattcc aaatcgccga aagcacggcc aaatccaagc agggcttttt ggagatgccc
     1381 gatttgctga aagaagtcgt acagcgcatc gatatgctct actcgcgcga caatcccgat
     1441 gaagttaccg gcgtgccgac ggggttcatc gacctcgaca aaaaaacctc gggcctgcaa
     1501 cccggcgacc tgattatcgt cgccggtcgt ccttctatgg gtaagaccgc cttttctatc
     1561 aatatcgccg aacacgttgc cgtagaaggc aagctgccgg ttgccgtctt ctcaatggag
     1621 atgggcgggg cgcaactggt catgcgtatg ctcggctcgg tcggacgctt ggatcaaagc
     1681 gtcctgaaaa ccggcaggct cgaagacgaa cactggggtc gtctgaacga agcagtcgtc
     1741 aaactctccg acgcgcccgt gtacatcgac gagaccccgg gtttgactgc gctcgaactg
     1801 cgcgcccgcg cccgtcgtct cgcccgtcaa tttaacaata agcttggatt aatcgtcatc
     1861 gactacctgc aactgatgtc gggttcgggg cgttcggaca accgagcttc ggagctggga
     1921 gagatttcac gttcgctcaa agcgttggcg aaagaattgc aagtccccat catcgccctg
     1981 tcgcaattga gccgtaccgt cgaatcgcgt accgacaaac gcccgatgat gtccgacctt
     2041 cgcgagtccg gcgcaatcga gcaggatgcc gacctgatta tgtttatgta ccgcgacgaa
     2101 tactacaacc aagactcgcc catgaaaggc cttgccgaat gtatcatcgg caaacaccgc
     2161 aacggtcccg tcggtaaaat cttcctcaca tggacgggac aattcaccaa attcgacaat
     2221 gctgcctata ttcccgagga ggcaaagata gaagattaaa tggctatata aaaatttatt
     2281 aggcgaaatc aggcaaaatc gtttaaaatc atgctgagag attgccctaa aaaataaaac
     2341 gcggtcttga ggcatttttg cattcagccc gcatataatt gaaaatacat taccagttaa
     2401 cgtcctatcg cttatgtgta cacgaaaaca acaaggtttc acgctaacag agctgctcat
     2461 cgtgatggtc attgcagcca ttatggcgat gatagccctc cccaatatga gccaatggat
     2521 tgcatcccgc cgcattgcca gtcacgcgga gcggattgcc aaccttttgc gtttctccag
     2581 gggcgaagcc gtccggctca atctccctgt ctatatctgt cctgttcaag ttaaaaaaga
     2641 cggtacggtc aacaataaat gtgactccgg caagaagggg cagggaatgt tggctttcgg
     2701 cgacaaaaac ggcaataagg gatatgacaa tgatacggcg gatgttcttc tccgcagtgt
     2761 ggtattgaat gatgatatca atgataagcg gattaattat gccttcaacc atatcgcttt
     2821 tggtcagact cagccgacca ccgaccgtgt agtttggaca ttcaatcaaa acgggacgtt
     2881 cggttatacg aaagaccagc atcttacaaa acaatccagc tttttttatt ccgacggtta
     2941 tatccaaatc gtgctgacag atgcgagagc agtttcagat gccgataaga aattccgttc
     3001 ggcggtggtt ttgattgaca gcagcggcag ggtcgaagtt tgccctaggg gtgatcggcg
     3061 tactcggcgt actatgtgcc agtataaata acagtttcag ttttaaaaat gaatatgaag
     3121 aataatgatt gcttccgcct gaaagattcc cagtccggta tggcgctgat agaagtcttg
     3181 gttgctatgc tcgttctgac catcggtatt ttggcgttac tgtctgtgca gttgcggaca
     3241 gtcgcttccg tcagggaggc ggagacacaa accatcgtca gccaaatcac gcaaaacctg
     3301 atggaaggaa tgttgatgaa tccgaccatt gattcggaca gcaacaagaa aaactataat
     3361 ctttacatga gaaaccatac actatcagct gtggatggcg attttgcgat tgatgccata
     3421 aaaactaagg cgcagttggc agaggaacaa ttgaagagat ttagttatga gctgaaaaat
     3481 gccttgccgg atgcggcagc catccgttac gccgtctgca aggattcgtt gggtgctgcg
     3541 ccgacattgt ccgccggcag tactttttct ccaaattgcg ataataaggc aaacggagat
     3601 actttaatta aagtattgtg ggtaaatgat tcggcagggg attcggatat cgcccgtacg
     3661 aatcttgagg tgagcggcga caatatcgta tatacctatc aggcaagggt cggaggtcgg
     3721 gaatgagacg taaaatgcta aacgtaccaa aaggcagtta tgatggtatg aagggtttta
     3781 ccattattga atttttggtt gcgggcatgc tcagtatgat tgtcctgatg gcggtcggat
     3841 cgagttactt cacatcccgg aaattaaatg atgcggcaaa cgagcgtctt gctattcaac
     3901 aggatttgcg gaatgcggca acattaattg tccgcgatgc aagaatggcg gggagcttcg
     3961 gttgtttcaa tatgtctgag cataccgaaa aggatgttgt ttccgatgtg gcgcaaaaaa
     4021 accgtctttt ttccttaaaa aggaacagta caaataaact tatccccata acggaatctt
     4081 taaatatcgg atatccgggt tttacccagc gccttaacgc attgattttc caatacggaa
     4141 tcgatgatgt taatgcaagc gccgatacta ccgtcgtcag cagctgtgcc aaaatagcaa
     4201 aaccgggtaa gaaaatatct accttgcaag aagcaaagag tgcattacag attactaatg
     4261 atgataaaca aaatggaaat atcacccgtc aaaggcatgt ggtcaatgcc tatgcggtcg
     4321 gcaggattgc cggtgaggaa ggtttgttcc gcttccaatt gaatgatgat gggcagtggg
     4381 gtaatcctca gttgttggtt aaaaagatta ataaaatgga tatacggtat atttatgttt
     4441 ccggctgtcc tgaagatgac gatgccggca aagaggaaac attcagatat acggataaat
     4501 tcaacagcgc ccaagatgct gttacgcccg ccggggtgga ggttttattg agtagcggta
     4561 ctgataccaa gattgccgct tcttcagaca atcatattta tgcttaccgt atcgatgcga
     4621 caatacgcgg gggaaatata tgcgcaaaca gaacgctttg acgggaatcc cgacttctga
     4681 cggacagagg gggttcgcgc tgtttatcgt gctgatggta atgatcgtcg tggctttttt
     4741 ggttgtaact gccgcgcagt cttacaatac cgagcagcgg atcagtgcca acgaatcaga
     4801 caggaaattg gctttgtctt tagccgaggc ggctttgcgg gaaggcgaac ttcaggtttt
     4861 ggatttggaa tatgatacgg acagtaaggt tacatttagc gaaaactgtg gaaaaggtct
     4921 gtgtaccgca gtgaatgtgc ggacaaataa tgataatgaa gaggcttttg acaatatcgt
     4981 ggtgcaaggc aagcccaccg ttgaggcggt gaagcgtttt tgccctgcaa attctaccga
     5041 cctgtgcatt gacaagaaag ggatggaata taagaaaggc acgagaagcg tcagcaaacc
     5101 accgcgctat attatcgaat atttgggcgt gaagaacgga gaaaatgttt atcgggttac
     5161 tgccaaggct tggggtaaga atgccaatac cgtggtcgtc cttcaatctt atgtaagcaa
     5221 taatgatgag taataaaatg gaacaaaaag ggtttacatt gattgagatg atgatagtcg
     5281 tcgcgatact cggcatcatc agcgtcattg ccataccttc ttatcaaagt tatattgaaa
     5341 aaggctatca gtcccagctt tatacggaga tggtcggtat caacaatatt tccaaacagt
     5401 ttattttgaa aaatcccctg gacgataatc agaccatcaa gagcaaactg gaaatatttg
     5461 tctcaggcta taagatgaat ccgaaaattg ccgaaaaata taatgtttcg gtgcattttg
     5521 tcaataagga aaaaccaagg gcatacagct tggtcggcgt tccaaagacg gggacgggtt
     5581 atactttgtc ggtatggatg aacagcgtgg gcgacggata caaatgccgt gatgccgctt
     5641 ctgcccgagc ccatttggag accttgtcct cagatgtcgg ctgtgaagcc ttctctaatc
     5701 gtaaaaaata gggctgtttt gccgatgccg tctgaagatt aattttcaga cggcattttt
     5761 atgggtatag tggattaaca aaaatcagga caaggcgacg aagccgaaga cagtacaaat
     5821 ag
//
LOCUS       DAWEWP010000124         5805 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWEWP010000124 DAWEWP010000000
VERSION     DAWEWP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.124"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(238..345)
                     /locus_tag="WGN53_001616"
                     /pseudo
     CDS             complement(238..345)
                     /locus_tag="WGN53_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(391..1119)
                     /gene="pdxJ"
                     /locus_tag="WGN53_001617"
     CDS             complement(391..1119)
                     /gene="pdxJ"
                     /locus_tag="WGN53_001617"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ0140589.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1143..1934)
                     /gene="recO"
                     /locus_tag="WGN53_001618"
     CDS             complement(1143..1934)
                     /gene="recO"
                     /locus_tag="WGN53_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ0140590.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(1968..3095)
                     /gene="pheA"
                     /locus_tag="WGN53_001619"
     CDS             complement(1968..3095)
                     /gene="pheA"
                     /locus_tag="WGN53_001619"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ0140591.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3134..4366)
                     /locus_tag="WGN53_001620"
     CDS             complement(3134..4366)
                     /locus_tag="WGN53_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ0140592.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYHVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            complement(4886..5740)
                     /locus_tag="WGN53_001621"
     CDS             complement(4886..5740)
                     /locus_tag="WGN53_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0140593.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGVGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT         1354 a         1686 c         1574 g         1191 t
ORIGIN      
        1 acataatcat cgcctcaata tttaatatcc caaaatatag atttaaaacc atcgcctttg
       61 tggtttgtca gcattcctaa ttgagataga aagtcaaata atttcttgtt tctccggctg
      121 ccatcaagat ggtttagatt aagacctcga acaatacaat acccaatact ctgtactgta
      181 ttgtgaattt cttctatcaa actattattg tttgcagata aaatatactt ttgattcata
      241 tagtgaatta aatttaaacc agtacggcgt tggcttgcct tgccgtacta tttgtactgt
      301 ctgcggcttc gccgccttgt cctgatttaa atttaatcca ctataacata tccctgtcgt
      361 gaaatgctgt ctgaaagggt ttgcctgccc ttacggcagc agccttgccc tgaacatcgc
      421 ctccttcatt tggcgcacgg cttcgggcag tccgaggaag agggcttggg caatcagcga
      481 atgcccgatg ttcagttcgc ggatggcgag gatttgggcg atgggggtaa cgttgtgtat
      541 ggtcagtccg tgtccggcgt tgacgaccaa gcccaaatcg ccggcaaaat gcgcgccgtt
      601 ttgcagacgc tcgaattgct tgagttgttc ggcgtggctg cgcgcgtcgg catacgcgcc
      661 tgtgtgcagc tcgacaacgg gcgcgccgac atcataggcg gcttggattt gcctgtcgtc
      721 ggcatcgata aagagagaga ctcggatgcc tgcgtcggtc aggattttgg tgaacccggc
      781 gattttttcc tgttgcgcca atacgtccaa gccgccttcg gtcgtgattt cctgacgttt
      841 ttcaggcacg atgcacacgt cttccggcat cactttaagc gcgttttcga gcatttcttc
      901 cgtcaacgcc atttcaaggt tcaggcgcgt gcggatggcg tttttgacgg caaacacatc
      961 cgcgtctttg atgtggcggc ggtcttcgcg caggtgcatg gtaatcaaat ccgcaccgtg
     1021 cgtttcggca accagtgccg cctccacggg gctgggataa gtcgtgccgc gcgcattgcg
     1081 gacggtagcg atgtggtcga tgttgacacc taaaagcata atctttcctt ttatttctgc
     1141 cttcagacgg catttgaagc cgtgccgtcc gaagtcggga cggtttcccg ggcggtttct
     1201 ttgcggtcaa actgccgtat ctgttccaac acctgccgcg atttcagccc ctcgggcaac
     1261 aggtggcgga taaaaagccg tgtgattttc aatgcctgtt gcaggctttc ggcggtgcgg
     1321 aaactgcctt cgcgcaaatc gatcaggctt tgcccttcga caattgcgcc ggcttcgtgc
     1381 gaaagcgcgt ctgcgtcctc gcatacgggc atgaccgcct cttcgggcat cagccggtag
     1441 gttttgtccg ccaaaatatc gccgcccgtg ccgtcggcgt gcaaatcggg ggcaacgccc
     1501 aacaggttca gcagccgcca ctcgaaacgg cgcaagtcgt cgatataagc ggctttgcag
     1561 cacaccgcct ccatcacttc cgccaacgcg tcgtataact cgggcaacgg atcttcgcgg
     1621 acggtcaact tcagcatcaa ctcgttcaca tacaatccgc tgaacaaagc cctaccctgc
     1681 ggctgcggcc acccgcctat ccattcggcg cgatgtaggg tcttcaactc ttgcgaacca
     1741 taccaagacg cactcaccgg cacaaacggc accaacacgc cgcgcaattc gctctgccgt
     1801 ttgcgcgcgc ttcttgccaa aagtgccaca cgcccgtaac ggcggctgaa cgcttcgacc
     1861 cgcaggctgc tttcgcgcca gggcgaggaa gccaacagga agacgggttc gtggttgact
     1921 cggtgttcgg acatcatgcc tgaaatgccg tctgaacgct gccgccgcta caaaacggcg
     1981 gtcgggtacg aaccgatgac tttgacgaac gaagcgcgtt cgcccaagcg ttccaatgcc
     2041 gtctgaatct gcgcgtcccg gcggtgtcct tcgatgtcga tgaagaacag gtattcccac
     2101 aaaacggatt tactcggacg gctctcaaac ttggtcatgg aaatacccga ctcagtcagc
     2161 ggttgcagca gcgaggcaac cgcgcctgcc cggttgggcg cggaaacggc cagcgaagtc
     2221 ttgtcgctgc cgcttgcacc ggtttcgtga tgtcccatca ccaagaagcg cgtggtgttg
     2281 ttcggttcgt cttcgatgca ctcggcaacc atatcgagtc cgtagatttc cgccgccgtg
     2341 cgtccggcga tggcggcaac cgtaccgtcg tccgattcgg caaccagcct tgcggcttcg
     2401 gcattgctgg acacggcaat ccgttcggcg ttgggcaggt gtctgcccaa ccagtcgttg
     2461 cactgcgcca acgcctgcgc gtgggaaaag actttggcaa tgccttcggt gctgccgttg
     2521 tttttacgca aaaggttgtg gtggatgcgc aaaacgactt cgccgcacgc ctgcaacgcg
     2581 gtaacggcaa gcaaatccaa cgtgcgcccg accgagcctt cggtcgagtt ttccacgggc
     2641 gcgaccagat aatccgcctg acgggtttca acctgcttga agcagttgtc tatggtcgga
     2701 cacgccatgg tgtgcgcggc gtgtccgaaa tgcttgatcg ccgcctgctg ggtaaacgtg
     2761 ccctgcggcc ccagataggc gatggtcagc gggcgttcga cggcgaggca ctcgctcatc
     2821 acttcccgaa acaggcgtgc taccgattcg tcgggcagcg ggcctttgtt caaatcctga
     2881 atgcggcgca acacggcgac ttcgcgttcg gggcggtaca ctgcgcccgt gcctttcagc
     2941 tcgccgatgg cgtgggcgtg ttgcgcacgt tcgttgagca ggcgcaggat ttcggcatcg
     3001 atggtgtcga tggcgttgcg gtggggaagg aggagttcgt cgatagtttg ggacataatt
     3061 tcgcctgaac agtaatttat cgtgcattct aacatcaaat gccgtctgaa acggtttcag
     3121 acggcatttt ccgttaaagg tattcgctct gttcgttttc cttccacgcc cggtgcgaac
     3181 acagccacag aagcgcaatg ccgcaagagg tggacgcggt catcgttgcc gccatcacgg
     3241 ttgccgaacc gtcgtgcaag aaggtcgccg ccatacccac ccccgcgccg attaaggact
     3301 ggaacacgcc caacacggcg ttcgcgctgc cgccctcttc tttgaaataa gacataaagc
     3361 atgcctgcgt gttcgcgccg accaagccct gcgtaccgac ggaaaacatc acgcacgcaa
     3421 ccagcagcca aaacgggggc aacccgaaaa acagcacggc ggcgagttgg gacaggttgg
     3481 cggcaaattg gacgacaatc ccccacagca ggatgctttg cggatgcgcg ccggttttga
     3541 gccgccacgc ggtaatacgg ttgaaaaaca tcatcgtgat gatgttgagc gcaaacgccc
     3601 aagcgtactg gtgcggcgta acgtggtaga gctgctggta cacgaaggaa gattcggtca
     3661 gaaaggcgaa catcgaaccg aagctgaatg cctgaaaaaa cagataaccc atcgcggcac
     3721 gggttttcaa tacgcgcttg aaccgccccg ccaccagccc gaacacgtcc ctgccgattt
     3781 tgccgccgac ggcgggcttg ggcaggaaat actgtaccaa accgagcagc accagcgaat
     3841 acgccgccag aaaaacaaaa atcgcctgcc agccacccaa gccctgcaac aatgcgccga
     3901 ccatgggtgc gaccagcggc acaaccatca aaatgatgcc gataagggca aacatctggg
     3961 cggctttgcg tccggaataa taatcgcgca ccattgcgcc gacgatgacc acagtcatgc
     4021 ccgcaccgaa tgcctgcacg acgcgcaggt tgaggagctg ttcggcactc gaagcaaata
     4081 cgatggcggc aacggcaagg cagtagacgg ccagtccggt cagcgcgacg ggtttgcgcc
     4141 ctttgatgtc ggacaccgaa ccgccgacca cctgtccgaa cgccgtgccg aacataaaca
     4201 aactcaggct ctgttcgatg cggtggacat ccgcgttcag cgactgcgcc atttcgggaa
     4261 tcgcgggcag gtaggcatcg atggaaaacg gcatcagcgt aaccagcatc gccatcaaaa
     4321 ccgccatcag tttttcgctc atttcaggat aatgggcaga aggcataaat tctcgtccga
     4381 ttcgcaaaca aaccgcccgg cggcggaaaa cacaatacgc ggaaacggaa aattttaaca
     4441 caccggcaca aacaggcggc aacaaaagcc ccggcaaaac cggacgaacc ggaacgatat
     4501 agtggattaa caaaaaccag tacagcgttg ccttgcctta gctcaaagag aacgattctc
     4561 taaggtgctt aagcacccag tgaatcggtt ccgtactatc tgtactgtct gcggcttcgt
     4621 cgtcttgtcc tgatttttgt taatccgcta taaaatcccg tcattcccgc gcaggcggga
     4681 atccggacat ccaacgctgc agcaatttgt cggaaataac cgaaactcaa aaaaccggat
     4741 gcccgcctgc gcgggaatga cgatgcaaag atggtcgtta tttcggataa atatccgtct
     4801 tttcgttccg gatacccgct ttcgcaggaa tggagatgga aagatttcag cctattcggc
     4861 aatcatcaaa taccgcttcc caactttatt tttgatgcga cagccggccg gcaaccaagg
     4921 tggcggcgat gacgacaaac acgcccaagg cggacacggg cgacaggtgt tcgcccaaaa
     4981 tcaaaaccgc cagcagcacg ccgacgacgg gttcgagcga aatcaacagt cccgacacgt
     5041 tggcaggaac acggctcatc cccttgttcc acagccaata ggcgtaccag ccgcacccca
     5101 cgcccaaata cagcagcgac aataccattc cgacgctcca gtccacggta taactttgcg
     5161 ccaaagcaag cgaaaacggc aggcacatca acgatgcggc ggcaatggaa acagatgtga
     5221 atgccggtgc gccgatgcgt gcaatcagcc tttgcgtcgg acgcatagcg gcacaaaagc
     5281 ccgcgcccgc caacaacacc agcaggcagc cgaaccagcc gacttcgccg ccctcttccg
     5341 caccgcccgc catcagcagc gcgacaccgg caaatgccgc cgcgccgcat atccagtggt
     5401 aggcacgcgc tttgtcgttg aagaaaaagt gtccgacaaa caccatcagc agcggctcga
     5461 gtccgacaat gaccgatgcg ctggcggcgg aagtgtattt caacccgaca aactgaagca
     5521 gcagggtcag cacatagttg acgaacgaca caatcagcaa cggcttccat tcctcacgcg
     5581 gaatcttgcc gacatgacgg cggcaggcgg gcagtgcagg cagcgcagca atcagcaggc
     5641 gcacgccgac catcaatgcg ggatcgatgc cgccatagac atatttggcg gcaataaacg
     5701 agctgctcca gataatcagg gcaaggattt ggtaaaacat agtgagtgat aaagtcagat
     5761 ggtcatacaa aagtatagcg gattaacaaa aatcaggaca aggcg
//
LOCUS       DAWEWP010000125         5694 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWEWP010000125 DAWEWP010000000
VERSION     DAWEWP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.125"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(81..842)
                     /locus_tag="WGN53_001622"
     CDS             complement(81..842)
                     /locus_tag="WGN53_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140594.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKVVPTYAPLSRPSETGTDASVP
                     KLDDASRNRVLKAYLKKWQEEHNAAVADCGEEIETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIEGGENADEAESGTNEPAVSENGENDAEETLMPV
                     QLNILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            942..1193
                     /locus_tag="WGN53_001623"
     CDS             942..1193
                     /locus_tag="WGN53_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140595.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEVA
                     LESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1245..2051
                     /locus_tag="WGN53_001624"
     CDS             1245..2051
                     /locus_tag="WGN53_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ0140596.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDEQAAK"
     gene            2358..3881
                     /locus_tag="WGN53_001625"
     CDS             2358..3881
                     /locus_tag="WGN53_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ0140597.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKSIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTGLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            3979..5391
                     /gene="trkA"
                     /locus_tag="WGN53_001626"
     CDS             3979..5391
                     /gene="trkA"
                     /locus_tag="WGN53_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ0140598.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1433 a         1716 c         1386 g         1159 t
ORIGIN      
        1 ttttgcgcct gttccatgag gctttcaagt cggatgagaa tgcaaatgcc gtctgaaacg
       61 gctttcagac ggcatggcaa tcagcgtttg tattttaact cgtacttgat gtcgttgagg
      121 attttgcgga catcgtgttc caacacgtct tcgactaccg cccccgcctg ctcgtgcagc
      181 atctgctgga gctgataggt gaaaaccgcc atctgctttt gcaccgccgt tcggatgatg
      241 ccgttgacgg tatcggtcag atgggggcgc aggcgtttga tcagccgttc ggtcagctcc
      301 tgttcggaca ggcagaacac ttcgcgccgg ttgacggctt tcgggttcag gatattgagt
      361 tggacgggca tcaacgtttc ttccgcatcg ttttccccgt tttccgaaac cgccggctca
      421 ttcgtgccgg attctgcctc gtcggcgttt tccccgcctt caatctgtcc ggtttcaaat
      481 tcgacactgt cttttttggt atcaaaccgg attctccgcc gcgattcgat gtgtttttcc
      541 gaaaccgaca tttgcaggga agcctgcgcg ttgagccagt tttcctgaag gacgatcatc
      601 gggtcggttt cgatttcttc cccgcaatcg gcaacggcgg cattgtgctc ctcctgccat
      661 ttcttcagat acgcctttaa cacacggttt cggcttgcat cgtccagttt cggcacagac
      721 gcgtccgttc cggtttcaga agggcgggac agcggcgcgt aagtcggcac taccttcata
      781 cggcgcgtct gacgcaggtt ttccaaacgt ttttcccaat tcggctcttt attcgcatcc
      841 attttcggct tccggtcctt aatctttgca agcagacaaa cccgcgccca aagcgcggtt
      901 tgatataatg gcgcatttta acagattcgc gaggatacat catgggcagc atcgaacagc
      961 gtttggaata tctggaagag gcgaacgacg tgctgcgtat gcagaaccac gtcctgtcca
     1021 ccgccttcaa agccttaatc cgcgcccttc ccgccgacac cgccgaagtc gcgctcgagt
     1081 ccatccagct tgcttttgag gacgccttgg cagaattgag ctatgaggac agcccgcata
     1141 cggatttgtt ccacgacgtt acttatgcgt ttttccgtga aaaagaacgt taattttatg
     1201 ttaaactgat tttttaggct ttttgattac cgaaaggaat tttgatgaat atgaaaaaat
     1261 ggattgccgc cgcccttgcc tgttccgcac tcgcgctgtc tgcctgcggc ggtcagggca
     1321 aagatgccgc cgcgcccgcc gcaaaccccg acaaagtgta ccgcgtggct tccaacgccg
     1381 agtttgcccc ctttgaatct ttagactcga aaggcaatgt cgaaggtttc gatgtggatt
     1441 tgatgaacgc gatggcgaag gcgggcaatt ttaaaatcga attcaaacac cagccgtggg
     1501 acagcctttt ccccgccttg aacaacggcg atgcggacgt tgtgatgtcg ggcgtaacca
     1561 ttaccgacga ccgcaaacag tctatggact tcagcgaccc gtattttgaa atcacccaag
     1621 tcgtcctcgt tccgaaaggc aaaaaaatat cttcttccga cgatttgaaa aacatgaaca
     1681 aagtcggcgt ggtaaccggc tacacgggcg atttctccgt atccaaactc ttgggcaacg
     1741 acaatccgaa aatcgcgcgc tttgaaaacg ttcccctgat tatcaaagaa ctggaaaacg
     1801 gcggcttgga ttccgtggtc agcgacagcg cggtcatcgc caattatgtg aaaaacaatc
     1861 cgaccaaagg gatggacttc gttaccctgc ccgacttcac caccgaacac tacggcatcg
     1921 cggtacgcaa aggcgacgaa gcaaccgtca aaatgctgaa cgatgcgttg gaaaaagtac
     1981 gcgaaagcgg cgaatacgac aagatttacg ccaaatattt tgcaaaagaa gacgaacagg
     2041 ccgcaaaata agcccgccca tccgaacaca atgccgtctg aagccctttc agacggcatt
     2101 gttcatcaat cggcctacaa tgaactgcct gctgatttct ccctaccgca aagcaacagg
     2161 caaagattac aaatatcaaa atccgagtaa aacagtattt tattaaaaca aattgataat
     2221 caagagatta gaattatgta ttgtctttac cgtacaaacg ctggcactat ttcaacctga
     2281 taaaaaacag ccttcaaaaa ggttgcttaa aacagcagca gacacttacc gccacaacct
     2341 tgaaaaggaa cacaatcatg accgtcatca aacaggaaga ctttatccaa agcatttgcg
     2401 atgccttcca attcatcagc tactaccatc cgaaagacta catcgacgcg ctttataagg
     2461 cgtggcagaa ggaagaaaac cccgccgcca aagacgcgat gacgcagatt ttggtcaaca
     2521 gccgcatgtg tgccgaaaac aaccgcccca tctgccaaga taccggtatc gcgaccgtct
     2581 tcctcaaagt cggtatggat gtgcagtggg atgcggacat gagcgtcgaa gaaatggtta
     2641 acgaaggcgt gcgccgcgcc tacacttggg aaggcaacac cctgcgcgcc tctgtcctcg
     2701 ccgatccggc cggcaaacgt caaaacacca aagacaacac ccccgccgtc atccatatga
     2761 gcatcgtgcc gggcggtaaa gtcgaagtaa cctgcgcggc aaaaggcggc ggctctgaaa
     2821 acaaatccaa actcgccatg ctcaatcctt ccgacaacat cgtcgattgg gtattgaaaa
     2881 gcatcccaac catgggcgca ggctggtgtc ctcccggcat cttgggcatc ggcatcggcg
     2941 gcacgcccga aaaagccgtg ctgatggcaa aagagtccct gatgagccac atcgacattc
     3001 aagagttgca ggaaaaagca gcgtcaggtg cagaattgtc caccaccgaa gccctgcgcc
     3061 tcgaactctt tgaaaaagtc aacgcgctag gcatcggcgc gcaaggcttg ggcggtctga
     3121 ccaccgtgtt ggacgtgaaa atcctcgatt acccgaccca cgccgcctcc aaaccgattg
     3181 ccatgattcc gaactgcgcc gccacccgcc acgtcgaatt tgaattggac ggctcaggcc
     3241 ctgtcgaact cacgccgccg cgcgtcgaag actggcccga tttgacttac agccccgaca
     3301 acggcaaacg cgtcgatgtc gacaagctga ccaaagaaga agtggcaagc tggaaaaccg
     3361 gcgacgtatt gctgttgaac ggcaaaatcc tcaccggccg cgatgccgca cacaaacgcc
     3421 tcgtcgatat gctcaacaaa ggcgaagaat tgcccgtcga tttcaccaac cgcctgattt
     3481 actacgtcgg ccccgtcgat ccggtcggcg atgaagtcgt cggtccggca ggtccgacca
     3541 cagccacccg catggacaaa ttcacccgcc aaatgctcga acaaaccggc ctcttgggca
     3601 tgatcggcaa atccgagcgc ggcgtggcca cctgcgaagc catcgccgac aacaaagccg
     3661 tgtacctcat ggcagtcggc ggcgcggcgt atctcgtggc aaaagccatc aaatcttcca
     3721 aagtcttggc gttccccgaa ttgggcatgg aagccattta cgaatttgaa gtcaaagaca
     3781 tgcccgtaac cgtcgccgta gatagcaaag gcgaatccat ccacgccacc gccccgcgca
     3841 aatggcaggc gaaaatcggc atcatccccg tcgaatcttg aggcgccatg ccgtctgaac
     3901 acaaaatctg ccttcagacg gcatttccgc ccccggttgc ggtacaatcc accatttcat
     3961 cactcggcga cccacaccgt gaaaatcctc attttaggca acggacaggt aggttctacc
     4021 gtcgcacaaa accttgccgc catacccaac aacgacgtaa ccgttatcga catcgacgaa
     4081 aaagcattgc aggaaacagg cagccgcctc gatgtccaaa ccgttttcgg caacggcgca
     4141 tcccccttca cattagaacg cgccggcgcg gaagatgccg acttgctgct cgcgctctcc
     4201 cgcagcgacg aaaccaacat cgtcgcctgc aaagttgccg ccgacctgtt caacatcccc
     4261 ggccgcatcg cgcgcgtccg ttccagcgaa tacctcgaat acctcagccc caagctcgaa
     4321 aacaacgaaa acggcagcct ttccatattc ggcataaccg aaaccatcag ccccgaacag
     4381 ctcgttaccg aacagcttgc cggcctgata gactgcccgg gcgcattgca ggttttacgt
     4441 tttgcagacg accgcgtgcg gatggtcatc atacaggcgc ggcgcggcgg actgcttgtc
     4501 ggacgcagca ttgccgacat cgcccaagat ttgcccgacg gggccgactg ccaaatctgc
     4561 gccgtttacc gcaacaaccg cctcatcgtc cccgcgccgc aaaccgtcat catcgaaggc
     4621 gacgaaatcc tatttgccgc cgccgccgaa aacatcggcg cggtcatacc cgaattgcgc
     4681 cccaaagaaa ccagcacccg ccgcatcatg attgccggcg gcggcaacat cggctaccgt
     4741 ctcgccaagc agctcgaaca cgcatacaac gtcaaaatca tcgaatgccg gccgcgccgt
     4801 gccgaatgga tagccgaaaa cctcgacaac accctcgtcc tgcaaggttc ggcaaccgac
     4861 gaaaccctgc tcgacaacga atacatcgac gaaatcgacg tattctgcgc cctgaccaac
     4921 gacgacgaaa gcaacattat gtccgccctt ttggcgaaaa acctcggcgc gaagcgcgtc
     4981 atcggcatcg tcaaccgctc aagctacgtc gatttgctcg aaggcaacaa aatcgacatc
     5041 gtcgtctccc cccacctcat caccatcggc tcgatactcg cccacatccg gcgcggcgac
     5101 atcgttgccg tccaccccat ccggcgcggc acggcggaag ccatcgaagt cgtcgcacac
     5161 ggcgacaaaa aaacttccgc catcatcggc aggcgcatca gcggcatcaa atggcccgaa
     5221 ggctgccaca ttgccgccgt cgtccgcgcc ggaaccggcg aaaccattat gggacaccat
     5281 accgaaaccg tcatccaaga cggcgaccac atcatctttt tcgtctcgcg ccggcgcatc
     5341 ctgaacgaac tggaaaaact catccaggtc aaaatgggct ttttcggata aaccgcccca
     5401 ttccggacat attgccgcca agcggtatgg aagcggaata atggtaggtg ggcttcagac
     5461 ggcatccgcc ctccccgtca ttcccgcgta agcgggaatc cagaccttgg gatagcggca
     5521 atattcaaag gttataaaag acccgtcatt cccgcgcagg cgggaatcca gaccttggga
     5581 tagcggcaat attcaaaggt tagctgaagc tttagagatt ctagattccc gtttccacgg
     5641 gaatgacgaa aggttgcggg aatccaaacc attgggcaac agcaatattc aaag
//
LOCUS       DAWEWP010000126         5618 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWEWP010000126 DAWEWP010000000
VERSION     DAWEWP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.126"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="WGN53_001627"
     CDS             complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="WGN53_001627"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ0140599.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGMHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(1445..2425)
                     /locus_tag="WGN53_001628"
     CDS             complement(1445..2425)
                     /locus_tag="WGN53_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ0140600.1"
                     /translation="MTAARAGLVRFNYLKVKQAVQSKQIKGFKLWKSYDEQLEILRSR
                     GLQIDDEKKALGYLRTIGYYRLSGYFYSFRQFAPESSKSRLDEFITDTRFEDVKSLYM
                     FDKKLRQLALDALERIEVALRVNIAHTLGRHDPLAYKDSKYSATGFDHAEWLDRYNRL
                     VEREKKTGFVKHHLEHYADLPVWVACEVWDFGTMSMLYKGMLNKDKDSIAKLYRLKDG
                     KHLQTHLHAFNFIRNVSAHHSRLWNRDITFKASLKGLSDAEWKSLPINKPFVYFCLMK
                     RMLDVICPRTEWGNRLLALLDEFPDVANEAVSLRDMGLENNPRDWRLWQG"
     gene            complement(2391..4670)
                     /gene="nrdA"
                     /locus_tag="WGN53_001629"
     CDS             complement(2391..4670)
                     /gene="nrdA"
                     /locus_tag="WGN53_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ0140601.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKDAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPNKFEGSKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQADIQDDGCAGGACKI"
     gene            5199..5510
                     /locus_tag="WGN53_001630"
     CDS             5199..5510
                     /locus_tag="WGN53_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ0140602.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         1406 a         1364 c         1310 g         1538 t
ORIGIN      
        1 gaacaataac cgatcctacc cgctgttttt cccatcatac aacatacaaa tgccgtctga
       61 aacatccggc ttcagacggc attttgtcaa aaaacgttta caactcaaaa tcgcccaaat
      121 catccgtatt cacttcagaa tcgatctgac cgatcaaata agaggatatt tccacttcct
      181 gcggcgcgac ctgtacgttg tcggacgaca gccacgcatt aatccacgga atcgggtttt
      241 gatttgcgcc ttcaaatccg gccggcaacc ccaccgcctg catacgcaga ttggtaatat
      301 attcgacgta ttgggataag atttctttgt tcaaaccgat catcgaaccg tccttaaaca
      361 aatatgccgc ccattctttt tcctgttccg ccgctttttt gaagagttgg aaacattcgt
      421 cctgcaactc ggcggcaatt tctgccattt cagaatcatc aacacccgaa cgcatcagat
      481 taagcatatg ctgcgtgccg gtcaggtgca gggcttcgtc gcgggcaatc agtttgatga
      541 ttttggcgtt gccttccatc aactcgcgct cggcaaaagc aaacgagcag gcgaatgaaa
      601 cgtagaaacg gatggcttcc aacacgttga cgcacatcag gcagagatag agttttttct
      661 tcaacccgcg caaagacaca gtaatgggtt ttccgccgac attatgcatc ccttcgccca
      721 acaggttgta atactgggtg tattcgatta agtcatcgta atagcaggca atgtcttcgg
      781 cgcgggcggt aatgtattcg ttttcgacaa tatcatcaaa cacgaccgac ggatcattca
      841 caatattgcg gatgatgtgg gtatagctgc gcgagtgtat ggtttcgctg aagctccacg
      901 tttcaatcca cgtttccagc tcgggaatcg aaaccaaagg cagcaaggca acattcggac
      961 tgcgcccttg gatggaatcg agcagtgttt ggtatttcag attgctgatg aaaatatgtt
     1021 tttcgtgttc aggcaggttg gcgtagtcga tacggtcgcg cgacacgtcg atttcttccg
     1081 gccgccaaaa aaacgacaat tgtttttcaa tcagtttttc aaatacctcg tatttctgct
     1141 ggtcataacg ggcgacattc acaggctgac cgaaaaacat cggctcattc agcgcgtcgt
     1201 ttttggtttt gggaaaggtg ctgtatgaca tgactaggtg ttcgcaggac atggttttct
     1261 caggtttatt taaatttagg aatttggatt tcagacagcc ttatatgttt taagtaaggt
     1321 catctgaatg tatctgtaca ttgaattctt gccttaaata tcgctgttag ttataatctt
     1381 ccccaacaac tcccccaact ttgccaacct acgcggttgg agcaacaagg ttgggggctt
     1441 tcttttatcc ctgccacaat ctccagtctc gcggattatt ttccaaaccc atatcgcgca
     1501 aacttacagc ttcattcgcg acatcgggaa actcatccag taaagcaaga agacggtttc
     1561 cccattcggt tcgcggacaa ataacatcca acatccgttt catcaggcaa aaatacacaa
     1621 acggtttatt gataggcaat gatttccatt ctgcatcaga caaacctttc aagctggctt
     1681 taaaagtaat atccctattc cataatcgac tgtgatgtgc cgaaacgttg cggataaagt
     1741 taaatgcgtg cagatgtgtt tgcagatgct taccgtcttt caaacgatac aatttggcaa
     1801 tagagtcttt atctttattc aacatccctt tatacagcat agacatcgtg ccgaaatccc
     1861 atacttcgca ggctacccag acaggtaagt cggcataatg ttccaaatga tgtttaacaa
     1921 aaccggtttt cttttcgcgc tcgaccagcc tgttatacct atccagccat tccgcatggt
     1981 cgaaacctgt ggcagaatat ttgctatctt tgtaagccaa tgggtcatgc cgtcctaatg
     2041 tatgggcaat atttacccga agcgctactt caattctttc cagtgcatct aatgccaatt
     2101 gccgcaattt cttatcgaac atataaaggc ttttgacatc ttcaaaacga gtatctgtaa
     2161 taaactcatc caacctgctc ttagaacttt caggagcaaa ttgtcggaaa gagtaaaaat
     2221 aaccgcttaa tcggtaataa ccgatggtgc gtaaatatcc taatgccttt ttctcatcat
     2281 caatctgcaa tcctctactt cttagaatct ccaactgctc atcgtaagat ttccaaagct
     2341 tgaagccttt tatctgcttg ctttgcacag cctgtttcac tttcaaatag ttaaatctta
     2401 caagccccgc ccgcgcagcc gtcatcctga atatcggctt gcgtatcgtc cgcgccgtcg
     2461 cgggtgttgt ggtagtacag ggttttgacg ccgtatttgt aagcggtcaa taagtctttg
     2521 agcatttgtt tcatggaaac tttgctgcct tcgaatttgt tcgggtcgta ggcggtattg
     2581 gcggaaatcg attgatcgac gaatttttgc attacgccga caagtttcaa gtagccttcg
     2641 ttgccgggaa gctgccacag ggtttcgtag gcgtctttca gggtttcaaa ttccggtacg
     2701 acttgtttca aaataccgtc ttttgatgct ttgaccgtta ccaatccgcg cggcggctcg
     2761 ataccgttgg tggcgttggc gatttgcgaa ctggtttcag acggcatgag tgcggttaga
     2821 gtagagttac gcaggccgta tttgacgatt tcggcacgca ggctttccca gtcgtaatgc
     2881 aaaggctcgc cgcagacggc atccaaatct tttttgtagg tgtcgatggg cagtttgcct
     2941 tgcgaataaa cggtttggtt aaagagcgta cacgcaccgt attctttggc aaggtttgcc
     3001 gatgctttga gcaggtaata ctgtatggct tcaaaggtac ggtgggtcag accgagcgcg
     3061 gagccgtcgc tgtagcggac accgtttttc gccagataat aagcatagtt aatcacgccg
     3121 atgccgagcg aacggcggcc catagtagag gtacgcgcgg cttctaccgg atatccctga
     3181 taatctaaaa gtgcatcgag cgcacgaacg gtcaagtcgg caagcccttc caattcgtcc
     3241 aagctgttta atgcgcccaa gttaaaggca gacagtgtac acagggcgat ttcgccgttc
     3301 ggatcgttga tattgtccag cggtttggtc ggcagggcga tttccataca caagttggac
     3361 tgatgaacag gcgcgacgcg cggatcgaac gggctgtgcg tattgcagtg atcgacgttt
     3421 tgaatgtaga tgcgcccggt tccggcacgc tcctgcatca gcgtggaaaa caggtcggca
     3481 gccggaatga tgcgcttgcg gatatcaggg tcttgctcgt atttcgtata gagccgctca
     3541 aattcgtctt ggtcggcaaa aaacgcttcg tacaatcccg gaacctcgtt gggcgaaaac
     3601 agcgtaatgt tgccgccctt aatcaggcgg gtgtacagca ggcggttgat ttgcacgccg
     3661 taatcaagct gacggatacg gttgtcttcc acaccgcggt tgtttttcaa caccagcagg
     3721 ctttcggctt cgatatgcca caaggggtag aacaaggttg ccgcgccgcc gcgcacgccg
     3781 ccttgcgaac aggatttgac cgccgcctga aacattttaa agaagggaat gcagccggta
     3841 tgccgcgctt cgccgccccg gatttcgctg tccaaaccgc ggatacgtcc ggcattgatg
     3901 ccgatgcccg cacgctggga aacgtatttc acaatcgcgc tggtagtggc attgatggaa
     3961 tccaaactat cgtcgcattc aatcagcaca cagcttgaga actggcgcgt aggcgtacgc
     4021 acgccgctca taatcggagt cggcagcgat actttaaatg tagaaacggc atcgtaaaac
     4081 cgtttgacgt aacccaagcg cgcctctttc gggtatttgc tgaaaaggca catcgccacc
     4141 aaaacatata aaaactgcgg cgtttcgtaa atttggcggg taacgcggtt ctgtaccaga
     4201 tatttgcctt cgagctgttt gacagcggca taggaaaagg acatatcgcg ttcgtggtcg
     4261 atataggcgt tcagttcgtc aaattcttcg cggctgtaat cctcaaggat atgcctgtcg
     4321 tattttccgg catcggtaag ttttttaacg tggtcgtaaa ggtgcggcgg ctcgtactcg
     4381 ccgtaggcta ttttacgaag atggaaaatc gccaaacgcg cggcaaggta ttggtagtcc
     4441 ggggtatctt ccgaaattaa atcggcagcg gctttgatga tggtttcgtg gatgtcgtcg
     4501 gtgcggatgc cgttgtagaa ctggatgtgc gatttcaact cgacctgaga tacggaaaca
     4561 ttttccaatc cgtccgccgc ccaagtgacg acacggtgaa tcttatccaa atcaatggct
     4621 tctaatcttc cgtctcgttt ggttactttc aaatcagtcg gtgtattcat cgcttcctct
     4681 tccactcttg atattcaaga cacagtcttt tcaaataaat taaggcagac aatatagtgg
     4741 atttttggca ttttctccag tcttgacaac ggttgtattt ttcagtttca ggcaatgcgc
     4801 gataaaaccc gcctgtcgta tttttcctat aatatttgtt ttatctgata attctttacc
     4861 gataaaaaac gggtaaattt tttgcctttt gaccggctcc ggctacaagg cggtgaaata
     4921 aggattttcc gacgaaaaag gaaagcttcc tgttttctgc ccctgcaaat tgttaaattt
     4981 tgcaaagtat gattttgccg cgccgccgcc gacaaattcc attttcttac cgattggaat
     5041 ttattattga gattaatgtg ttatttgaat ctgcatatca aacggcaagt tttgtcggct
     5101 gaatgagtct gaaactgccg acaatttgcc cgttccatcc ttccatccta tactggaaag
     5161 aatgacaaac tgaaaggatc gtcatgtctg tcaaaaagat gcttgccata ctgttgtctg
     5221 caatattggg actggtatcg acaactgccg ctgccggtac gtcagaaccc gcccaccgcg
     5281 ataccaaaca tatccgcaag gcaaacaagc agatgctgca ccccgaatgc aggaaatatt
     5341 tggaacgccg tgccgcgtgg taccgatcgc aaggcaacgt gcaggaattg cgcgaaaaca
     5401 aaaaggcgcg caaagcattc cgctccctgc cttatgcgga acagaaaatc caatgccggg
     5461 cggcttatga ggctttcgat gatttcgacg gcggcagttt ccgccgttaa tcccatataa
     5521 aatatgccgt ctgaacacag gttcagacgg cattttccat agatacaaca aactatcctt
     5581 acggcatttc gtttgaggtg ctcccgttgc cgcagcct
//
LOCUS       DAWEWP010000127         5584 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWEWP010000127 DAWEWP010000000
VERSION     DAWEWP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.127"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..369)
                     /locus_tag="WGN53_001631"
     CDS             complement(<1..369)
                     /locus_tag="WGN53_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
                     /protein_id="HEZ0140603.1"
                     /translation="MEDKQGMKKAVAGVMTDASSDGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYEKYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGF"
     gene            471..686
                     /locus_tag="WGN53_001632"
                     /pseudo
     CDS             471..686
                     /locus_tag="WGN53_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(838..1896)
                     /gene="trpD"
                     /locus_tag="WGN53_001633"
     CDS             complement(838..1896)
                     /gene="trpD"
                     /locus_tag="WGN53_001633"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ0140604.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(1961..2551)
                     /locus_tag="WGN53_001634"
     CDS             complement(1961..2551)
                     /locus_tag="WGN53_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ0140605.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVYHSGKGMFKGLPNPVTCTRYHSLVIERGTLPECLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEYGHDMLNNFFIEFQNFKPQKI"
     gene            2973..5249
                     /locus_tag="WGN53_001635"
     CDS             2973..5249
                     /locus_tag="WGN53_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0140606.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKILSGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
BASE COUNT         1440 a         1560 c         1274 g         1310 t
ORIGIN      
        1 gaaaccgaaa acttcttcgg cgagcatgga cgcggcgcga ataaaatctt cgtcttccaa
       61 aagacttaca cgaacgccgt atttatcgaa aaaggttttt tcatgaaatg aaaagctaat
      121 ttgatcgatg aacgccgaat cagatacacc gcgacgaagc ggaacgccta aaaggtttcc
      181 tttgccgtct gttatgtact tttcataaca ctcgaagact tcttgaaccc tgcccgccgt
      241 ttcggtttct gtaccccccc tgttagataa ggggggaaga tttgaagcgg ttgccggctt
      301 cctgccgtcc gatgacgcgt ccgtcatcac gccggcaacc gcctttttca tcccttgctt
      361 atcttccatg gtgcgaatcc tcaaaaacgg gcaaaaaaaa gccctgttac ttgtagaaag
      421 taaaggacgt taatttttgt taatcgtccc ttcttaggga cgcaatatat aaggtttata
      481 ccgtcgttgt tcctaatgcg caatcagcga cattgttgcc aattatccga aagaaagtta
      541 agcctgatgg cattgtatac acggatacct ttcgtagtta tgatgtgctt gatattagtg
      601 aatttagcca tttacgtaag tttaacggca ttcccaaaga gcatttgggg ctgcatttaa
      661 agaaatgcca atggcatttt aaatagaatc tcaaattcag attttaagac aattagttaa
      721 tgggaatttg gtctggttat ctagtacagc cccaagtttt tatataaaaa cagtttggta
      781 agttctcttc gtgaagtgct tatgttttgc cacaaaagtt ccataatcat ggcggcatca
      841 cccaagctcc atctttccaa gaaaatggca tctttgttgt ggttgaaaac cgacaaactc
      901 ctcttttttc gatttggccc tgcctgaatc gatggcttcc cgtgcggcag atatgccgtc
      961 tgaaagcgaa gcagcgacat ttccggcata cagggcggcg gcggtgttga gcaatacgat
     1021 atcgcgcgca gccccttctc ttccttccag cacctcattc attttcaaca aagattcctg
     1081 agtattggca actttgattt catccaaatt gcggcgggtt tcgataccga aatcttctgg
     1141 gcggatgtcg tattcgctga tttttccgtc tttgagctcg gcaacgcgtg ttttgcccgt
     1201 cagtgtaatt tcatccaaac cgccctcccc gcaaacaacc aaaacgtgtt ttgaaccaag
     1261 ttgttgcaag acccgcgaca aaatgccaca caaatcggtg tggaacacgc ccaaaagctg
     1321 gttcggcgcg cccgcaggat tcgttaacgg acccaatatg ttgaaaatac ttcggaaacc
     1381 gagcgaacgg cgtacagggg cgacatggcg catggcactg tggtgattgg gcgcgaacat
     1441 aaacccgatg ccggtctgcc tgatactttg ggcaacctgt tcgggagtca ggttgaggtt
     1501 tgcgcccatc tgctccacca cgtcagccgc accgctggag gaagagaccg accggcctcc
     1561 gtgtttggca accttcgcgc ctgccgctgc ggcaacaaac atcgaagtcg tcgaaatatt
     1621 gaaggttttc gcgccatccc cgcccgtacc gacgatatcg accagcccct ctgcattctc
     1681 cagcggcact tttgtcgcaa actcacgcat gacggctgca gctgcggtaa tttcggaaac
     1741 ggtttcaacc ttgatacgca atcctgtcaa aatggccgct atctgctccg gcagaacctg
     1801 tcctctcata atctgacgca tcaagtcggt catttcatcg taaaacaact cgttattgct
     1861 gattaatcgt tcgatggcct gttgcggtgt aatcattttt ttgtcctccg ttcaatattc
     1921 ggacgaaaat gccgtctgaa gggcttcaga cggcatcacg tcagattttt tgcggtttga
     1981 agttttgaaa ttcgataaaa aaattgttca acatatcatg tccgtattcg gtcagcagag
     2041 cttcagggtg gaattgtacc ccctcaacgg catattcctt atggcgcacg cccataatct
     2101 cgccgtcttc cgtccatgcc gtgatttcca aacattcggg cagcgtgccg cgttcgataa
     2161 cgaggctgtg ataacgcgta caggtaaccg gattgggcaa acccttaaac atacccttgc
     2221 ccgaatggta cacgggcgac accttgccgt gcatcagcgt tttggcgcgg actatcctgc
     2281 cgccgaacgc ctcgcctatc gtctgatgcc cgaggcacac gcccataatc ggcagcctgc
     2341 cggcaaaatg gcgcatcgcc gccacggaaa tccccgcttc tttgggcgaa cacggaccgg
     2401 ggccgataac gagatattgc ggattcaatg cctcgatttc ctccaacgta atatcatcgt
     2461 tgcggcgcac ggcaacttcc tgccccaatt cagtgaaata ctggacgatg ttgtaagtaa
     2521 aactgtcgta attgtcgata aacaaaagca ttttgtgatt aacctattga tttataattt
     2581 gtttgttatt aactgcattt ccgatacccc catccatacc ctcttatatc ttagcgtgcc
     2641 cgatgcgccc tcgtgaacct ggcgcagagc ctcgcgttct gacaagtacg ccattttgcc
     2701 accttttcga ccgttcggga acgtgccgtt tttatgcact cataaatttt ggttatcccg
     2761 cagccgaaca taatgggcaa acacagcgga atctgcgtca acatcaaccg cgcccccgaa
     2821 ggacggcgga tacaaatgga caccgcccga tgccgcaacc gtttcatttg aaaaactttt
     2881 gcctatgccc tctgagtgtt ctcaaaatct tatgatataa ggaatgcaat gttatataat
     2941 aacaaacttt tgaaatgcga cgagaagcca aaatggcaca aactacactc aaacccattg
     3001 ttttatcaat tcttttaatc aacacacccc tcctcgccca agcgcatgaa actgagcaat
     3061 cggtggattt ggaaacggtc agcgtcgtcg gcaaaagccg tccgcgcgcc acatccgggc
     3121 tgttgcacac ttcgaccgct tccgacaaaa tcctctccgg cgataccttg cgccaaaaag
     3181 ccgtcaactt gggcgatgct ttggacggcg tgccgggcat tcacgcctcg caatacggcg
     3241 gcggcgcgtc cgctcccgtt attcgcggtc aaacaggcag gcggattaaa gtgttgaacc
     3301 atcacggcga aacgggcgat atggcggatt tttcgcccga tcacgccatt atggtagata
     3361 ccgccttgtc gcaacaggtc gaaatcctgc gcgggccggt tacgctcttg tacagctcgg
     3421 gcaatgtggc ggggctggtc gatgttgccg atggcaaaat ccccgaaaaa atgcctgaaa
     3481 acggcgtatc gggcgaactc ggattgcgtt tgagcagcgg caatctggaa aaactcacgt
     3541 ccggcggcat caatatcggt ttgggcaaaa actttgtatt gcacacggaa gggctgtacc
     3601 gcaaatcggg ggattacgcc gtaccgcgtt accgcaatct gaaacgcctg cccgacagcc
     3661 acgccgattc gcaaacgggc agcatcgggc tgtcttgggt tggcgaaaaa ggttttatcg
     3721 gcgtagcgta cagcgaccgt cgcgaccaat atggtctgcc tgcccacagc cacgaatacg
     3781 atgattgcca cgccgacatc atctggcaaa agagcttgat taacaaacgc tatttacagc
     3841 tttatccgca cctgttgacc gaagaagaca tcgattacga caatccgggc ttgagctgcg
     3901 gcttccacga cgacgatgat gcacacgcac acacccacag cggcagaccg tggatagacc
     3961 tgcgcaacaa acgctacgaa ctccgtgccg aatggaagca accgttcccc ggttttgaag
     4021 ccctgcgcgt acacctgaac cgcaacgact accgccacga cgaaaaagca ggcgatgcag
     4081 tcgaaaactt ttttaacaac caaacgcaaa acgcccgcat cgagttgcgc caccaaccca
     4141 taggtcgtct gaaaggcagc tggggcgtgc aatatttaca acaaaaatcc agtgctttat
     4201 ctgccatatc cgaagcggtt aaacaaccga tgctgcttga caacaaagtg caacattaca
     4261 gctttttcgg tgtagaacag gcaaactggg acaacttcac gcttgaaggc ggcgtacgcg
     4321 tggaaaaaca aaaagcctcc atccgctacg acaaagcatt gattgatcgg gaaaactact
     4381 acaaccatcc cctgcccgac ctcggcgcgc accgccaaac cgcccgctca ttcgcacttt
     4441 cgggcaactg gtatttcacg ccacaacaca aactcagcct gaccgcctcc catcaggaac
     4501 gcctgccgtc aacgcaagag ctgtacgcac acggcaaaca cgtcgccacc aacacctttg
     4561 aagtcggcaa caaacacctc aacaaagagc gttccaacaa tatcgaactc gcgctgggct
     4621 acgaaggcga ccgctggcaa tacaatctgg cactctaccg caaccgcttc ggcaactaca
     4681 tttacgccca aaccttaaac gacggacgcg gccccaaatc catcgaagac gacagcgaaa
     4741 tgaagctcgt gcgctacaac caatccggtg cggacttcta cggcgcagaa ggcgaaatct
     4801 acttcaaacc gacaccgcgc taccgcatcg gcgtttccgg cgactatgta cgaggccgtc
     4861 tgaaaaacct gccgtcccta cccggcaggg aagatgccta cggcaaccgt cctttcatcg
     4921 cacaggacga ccaaaatgcc cctcgcgttc cggctgcgcg cctcggcgtc cacctgaaag
     4981 cctcgctgac cgaccgcatc gatgccaatt tagactacta ccgcgtgttc gcccaaaaca
     5041 aactcgcccg ctacgaaaca cgcacgcccg gacaccatat gctcaacctc ggcgcaaact
     5101 accgccgcaa tacgcgctat ggcgagtgga attggtacgt caaagccgac aacctgctca
     5161 accaatccgt ttacgcccac agcagctttc tctctgatac gccgcaaatg ggccgcagct
     5221 ttaccggcgg cgtgaacgtg aagttttaaa atcggacagg caaacaaaaa aggttttcag
     5281 acggcattct gaaaaccttt tttcttttta atcaatttgt taggaaatat ggcaaagaca
     5341 aagaacaaac cactatgcca tcattcccat tttctttgtt tcatcattct tgctttcacg
     5401 ccgtcattct cgcaaaagca gaaaaatcaa aagcagaaac ccatcattcc cgcgcaggcg
     5461 agaatccaat tcattcggtt tcagccgttt ccgataaatc gccttagcat ttgggtttta
     5521 gattcccact ttcgtgggaa tgacggcgta aaggtttctg ttttttaagt tttaggtaac
     5581 taat
//
LOCUS       DAWEWP010000128         5539 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWEWP010000128 DAWEWP010000000
VERSION     DAWEWP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.128"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..558
                     /locus_tag="WGN53_001636"
     CDS             <1..558
                     /locus_tag="WGN53_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0140607.1"
                     /translation="DTERFFPQPGETADLRAKYGFADHETVFLFPSTGHTRKGLELLA
                     DFFEHTGLPVKLAVVGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVG
                     VESVLCGTRVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSD
                     PMRALNYNPSLSHHIDRLTDILASV"
     gene            complement(625..2277)
                     /locus_tag="WGN53_001637"
     CDS             complement(625..2277)
                     /locus_tag="WGN53_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0140608.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGVPWRWAGAALFAAAFVSMWYFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFAKQMPSETQPCGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGLVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRKLVGDYRNYLYDVLKIAS"
     gene            2447..3020
                     /locus_tag="WGN53_001638"
                     /pseudo
     CDS             2447..3020
                     /locus_tag="WGN53_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            3017..3580
                     /gene="gmhB"
                     /locus_tag="WGN53_001639"
     CDS             3017..3580
                     /gene="gmhB"
                     /locus_tag="WGN53_001639"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ0140609.1"
                     /translation="MKLIILDRDGVINQDRDNFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            3610..4353
                     /locus_tag="WGN53_001640"
     CDS             3610..4353
                     /locus_tag="WGN53_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0140610.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            4350..5042
                     /locus_tag="WGN53_001641"
     CDS             4350..5042
                     /locus_tag="WGN53_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0140611.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPEHIWFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPTPSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            5153..5338
                     /locus_tag="WGN53_001642"
     CDS             5153..5338
                     /locus_tag="WGN53_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140612.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRPSETRNP
                     PGPTCGMKRLSWREVFY"
BASE COUNT         1438 a         1672 c         1304 g         1125 t
ORIGIN      
        1 gatacggaac gcttctttcc acaacccgga gaaactgctg acctgcgcgc caaatacggc
       61 tttgccgacc atgaaaccgt tttcctgttc ccatcgaccg gccacacgcg caaaggtctg
      121 gaactgcttg ccgacttttt cgaacatacc ggcctgcccg tcaagcttgc cgttgtcggc
      181 tccccgctcc cccgccctat gaaaaacgtc gtcggactgg gcttctgcac cgatatgccc
      241 gaactctacc gcgccgccga ctttaccatt atggcctccc tgtatgaacc cttcgggctg
      301 gtcggtgtcg aatccgtcct atgcggcaca cgcgtcgtcc tctccgaaaa catggcatgt
      361 acagaggtca tgaacgaaga agccggattc tttttctcac gccaaaaccc ggaaaccctg
      421 gcgcaagccg ttgcccaagc cgtcagcctt aaaaaacagg gcggacaccg cctgtccgac
      481 ccgatgcgtg cactgaacta caacccgtcc ctgtcgcacc acatcgaccg actgaccgac
      541 atactggcat ccgtataaca aaatgccgtc tgaacgagtt ttcagacggc atttttgcaa
      601 ccgttgcagc cgtgcggcaa aaagctaact ggcaattttc aaaacgtcgt acagatagtt
      661 ccgatagtct ccgaccagtt tccggttcgg gcagtcttcc aaccctccgc cgcacaaacc
      721 gcgcatcgcc atattcgcac gcatgaagcc gtctttggct tccagaccag ccgcctccat
      781 cagcacgccg cctgcaaagg caaggtcgag gaaatcctgc cgctgtacga atccgccggc
      841 aatattgctc ctgacggcaa actgcgttac ataatccggc tgcgcgtaat cccatttctt
      901 cctgacggac acgccctcaa acggtacctg atgatcgccg aaataaccga aaacaaaggg
      961 tttgccgcgt tcgtgcagat aacggtcgaa actttccacc gctttgtcca aatcggcaat
     1021 gcgcccgatg tagtcgttaa gcgcggatac ggttttcgca ttcaaatcag gtgcatccaa
     1081 atcaaacaca ttgtcggtat ccgtccgata cggcccgtgc tccttcatgg tcagcacata
     1141 tacgaacatc ggctgccgca cgttttccaa atcgggatgg cgtttttcca aaatcatccg
     1201 cgcatactgc atcatttcct cactggaaat atgccacagg tttttgccca tcggcgcggg
     1261 atagcccaaa tcttgcggct ggaacatcag attaaagccg aaatggtcat atgctgcctt
     1321 ggcattgtag ttgcccttgg taaacggcga gagtgccacg caaaaataac cgtgttcgcg
     1381 caggttgcgg accaaaccgg tctgcaaatg cggcacgacc gagtaaaaca cgccacttgc
     1441 caacgcgccg aaatccgtgg acggaacacc cgccaaaaac gcaaattcgg acttccacgt
     1501 tgcgccgccg aaagtatgca cgcgcaaagg tgaggaaaat acggtatctt cctgccgccc
     1561 gaacattttc aaatcgggaa tcttggcggc agcaaagtcg aaacagtgcg gatcgagcgt
     1621 cgattccatc agggtaacga caatatcggg tttttcatcc gacatgccgc acggctgggt
     1681 ttcagacggc atctgtttag caaacgcttc cccgtcgccc tcgaacacgg gtacttggaa
     1741 aaacaccgcc cgacaggaca tcggcaggtt caaaaacacg tcgcgcccgt cgtccggcag
     1801 cgagtccagc cacgtcttta ccgcgcccgg atgtttggaa aaataccaca tcgacacgaa
     1861 cgccgccgca aacaacgccg cgcccgccca ccgccacggc acacccaaag aatccgcacc
     1921 gctccagccg aaaaccgcat atgccgccaa agccagcagc cccgccatac cgataaccgc
     1981 ttccttataa tgaaacagcg tttcccaatt ccgccagtcg gcaatcagca aaaagtcgga
     2041 aatcaacagc ggctgtttgt aataatggat tttcagcctg tggaacagta tcagcaccac
     2101 aaaccagacc gaagcgaaat tcaaggcgcg ctgccactga gccgtcaaca tcagcatacc
     2161 gcccgccaaa aagacaaaca gcgcggatgc gaaaaaatac gtccagcggt agtgcgacct
     2221 gataatcagc accaacgccg ccgttacaaa caaaaatagg aaaacatagg caaccatccc
     2281 gtcccactcc tcaaaacggc atacctgagc cgttgcgaat tattatcgaa agtgtgcatt
     2341 ataatgcagc acaggcgcat cactgccgat atttcagtat aattattcga gatggaatca
     2401 ctgaaaatgg tgaagttaca atgagccctt ttagaggaac taaaaagtga attataaaat
     2461 atacgatggt gcaaagatta ttaaaaaaga aaatttatca attggggatt attctcaaat
     2521 tgatgatttt gtatttttta attgtggaga gaaaagtatt ttaggaagtt ttgttcatat
     2581 atcatcattt accagtatta ttggtggtgg agaattttat atggatcatt tttcaggatt
     2641 aagcgcaggg tgtagaatta ttaccggtag cgatgatttt atgggggggg ggggggttaa
     2701 ccaatccaac agtcccatct aaatatacta atgttaaaaa atctagtgtt agaattggaa
     2761 aacatgcaat tttaggaaca aatgtaacag tattaccagg cgttactatt ggtgatgggg
     2821 ttgctgttgg agcaggggct atagtgagaa aaaatcttga gccgtggact atttatgtcg
     2881 gggtagattg taaaccactg aagcgcagat cacaagataa aatattggag ttagagaaac
     2941 atcttgtagc agaatttaat ctagatttat gtcctataat cccatccccg aacctgccca
     3001 aaccaaagga aacgcgatga aactcatcat tctcgaccgc gacggcgtca tcaatcagga
     3061 ccgcgacaac ttcgtcaaat ccgttgacga gtggatacct gtcgaaggca gcatggatgc
     3121 ggtggcattt ctgacgcagg caggctacac cgtcgccgtt gccaccaacc aatccggcat
     3181 cgggcgcaaa tattttaccg ttcaaaacct taccgaaatg cacgccaaaa tgcaccgcct
     3241 cgtccgtcag gcaggcggcg aaatcaacgg catctggttc tgcccgcaca ccgatgccga
     3301 caactgcaac tgccgcaagc ccaaaccggg tatgattgaa gacatcatcg gacgcttcaa
     3361 cgcccaagcc tcggaaacct ggctggtcgg cgacagcctg cgcgatttgc aggcaatcga
     3421 tgccgtcggc ggaaaacccg cgctggttct gaccggaaaa ggcaaaaaaa cgctctccca
     3481 acacggacac gaattacccg aacacacaca ggttttcgat accttgctcg atttctcaca
     3541 atacatcatg caggaaaacg ccgcaccgca agccgactga acataccgca tttccgacaa
     3601 ggcaaaacca tgctcatcat ccgcaacctg atttactggc tgatactctg ttccaccctg
     3661 attttcctct ttccctttat gctgctcgcc tcgcctttcc gggacggggc gcacaagatg
     3721 gcgcgggtct gggtcaaaat cctcaacctc tcgctcaaac acatcgtcgg gctcaaatac
     3781 cgcatcatcg gcgcggaaaa catccccgac cgccccgccg tcatctgcgc caaacaccaa
     3841 agcggctggg aaacgctcgc ccttcaggac atttttccgc cgcaggttta cgttgccaaa
     3901 cgcgagttgt tcaaaatccc ctttttcggc tggggcttga aactggtcaa aaccataggc
     3961 atagaccgca acaaccgccg cgaagccaac gagcagctca taaaacaggg gttggcgcgc
     4021 aaaaacgaag gctattggat taccattttc cccgaaggca cgcgccttgc gcccggaaaa
     4081 cgcggcaaat acaaactcgg cggcgcgcgc atggcgaaaa tgtttgagat ggacatcgtc
     4141 cccgtcgccc tcaacagcgg cgaattttgg ccgaaaaact cctttctgaa atatccgggg
     4201 gaaatcaccg tcgtcatctg tccgaccatc ccgcacgcaa gcggcagcga agccgaattg
     4261 atggaaaaat gcgaacatct catcgaaacg caacaaccgc ttatttccgg cgcaggcccg
     4321 tttgccgcca aaatgccgtc tgaaaccgca tgaccgcctt tgtccacacc ctttcagacg
     4381 gcatggaact gaccgtcgaa atcaagcgcc gtgccaagaa aaacctgatt atccgccccg
     4441 ccggcacaca taccgtccgc atcagcgtcc caccctgctt ctccgtctcc gctctaaacc
     4501 gctggctgta tgaaaacgaa gccgtcctgc ggcaaacact ggcgaaaaca ccgccgccgc
     4561 aaactgccga aaaccggctg cccgaacata tctggttcca cggcagacag cttgccctca
     4621 ccgcccatca agacacgcaa atcctgctga tgccgtctga aatccgtgtt cccgaaggcg
     4681 cacccgaaaa acagcttgcg ctgctgcggg actttttgga acggcaggcg cacagttacc
     4741 tgattccccg cctcgaacgc cacgcccgca ccacacaact gttccccacc ccctcctcgc
     4801 tgacctctgc caaaaccttc tggggcgtgt gccgcaaaac cacaggcata cgcttcaact
     4861 ggcggctagt cggcgcaccg gaatacgttg ccgactatgt ctgcatacac gaactctgcc
     4921 acctcgccca cgccgaccac agccgcgcgt tctgggcact gacccgccgc ttcgcaccct
     4981 acacgcccga agcgaaacag tggctcaaaa tccacggcag ggaacttttc gccttaggct
     5041 gacgcggccc gggcaggcat ccgccaattc agacctcctc ccaaacggac atccggaaag
     5101 cagcatcaca gccggcgaca cataccgctg ccgccgcttt cagacggcat ccgtgccgga
     5161 acaggcacgc gcccgcccga ttcaaaccgc gatgacgctt tgccgccggt tcggggcagg
     5221 atggcggcac acacgccgtc tgccgcgttt catttcacac cgcccttccg aaacccgaaa
     5281 cccgcccggt ccgacgtgcg gtatgaaacg cttaagctgg cgcgaagtct tttactgatt
     5341 tgcccgcgaa aatgccgtct gaaaggtttt cggacggcat ttttttgcgt ttcccaggat
     5401 ggcggcggat tcgtaaaagg cggtcagggt ggattgtagg atgggttgag acctgccgaa
     5461 tccgccgcat ctgccaaatc taccgccgtc attcctacga aagtgggaat ctagaacgcg
     5521 gggttaagaa aacctgcat
//
LOCUS       DAWEWP010000129         5456 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWEWP010000129 DAWEWP010000000
VERSION     DAWEWP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.129"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(145..1941)
                     /locus_tag="WGN53_001643"
     CDS             complement(145..1941)
                     /locus_tag="WGN53_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ0140613.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPAHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPNDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2399..3652
                     /locus_tag="WGN53_001644"
     CDS             2399..3652
                     /locus_tag="WGN53_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0140614.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3807..5318
                     /gene="lysS"
                     /locus_tag="WGN53_001645"
     CDS             3807..5318
                     /gene="lysS"
                     /locus_tag="WGN53_001645"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ0140615.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
BASE COUNT         1179 a         1383 c         1605 g         1289 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat atttgcttgc
       61 ggcaagatga aaacggtgcg ggatgttttg ggaaaccaat gccgtctgaa gggctttcag
      121 acggcatttt ttgcgccgtg ctgtttaacg cgccagcggt tcggtgcgtt tgatcagcca
      181 cgcttttgcc gcgccttcgg tcagcggctc gaggcggcgg cggacttcgg cgtggtagcg
      241 gttgacccag tcgatttcgc cgtcggtcat gagggcggtg tccatcaggc gggtgtcgat
      301 ggggcagagg gtcagggttt caaaacagag gaagctgccg aattcggttt cttgaggggc
      361 ggcgacggct tggttggcgg caaggttttc aatgcggatg ccccattttc ccgggcggta
      421 gagtccgggt tcgatggagg taaccatgcc ttttttcatg gcggtttcgg gcgtggcggg
      481 ggcggcgaag gcgatgcgct gcgggccttc gtggacgttg aggaaatagc ctacgccgtg
      541 tccggtgccg tggccgtagt cgcattgcgc ctgccacagg ggtttgcggc aaatcgcatc
      601 aatcagcggc gaggggatgt tttctgggaa cacggcttcg gcaagcgcga tatgggcttt
      661 gagaacgagg gtgttgtcgc gtttttgttc ggcggtgggt gtgccgacag ggacgacgcg
      721 ggtgatgtcg gtcgtgccgc ctttgtattg cgcgccggag tcgatgagca aaagcccgtt
      781 gccgctgatg gtgctgtggc tttcgggcgt tgcgctgtaa tgcggcagtg cgccgttggc
      841 gttgaagcct gcgatggtgt cgaaactcaa tgaaatgaag cctgggcgca cgctgcggtg
      901 gcgataaagc atggtgtcca cgtcgatttc ggtcaggctg ccgccgttgc cgatgatgtc
      961 ttcaaactcg gcgaagaaac cgcacaacgc cgcgccgtcg tgttccatcg cttcgcggat
     1021 gcgggcgatg tcggcttcgg atttgcagga tttgaacagc gtggacgggt tgattccctc
     1081 gataaggcgc acgctttcgg gcaggcgcac aagcgtgctg acggcggttt tgttcggttc
     1141 gataagcaac gcgccgccga tttgcgcgag tttgtcggca acttgggcgt aaggctcgac
     1201 cgtgatgcct gcggtttgca gcgcggcggc ggcttcggcg ttcagacggc atcggtcggt
     1261 aaacaggacg gcgttgtctt tgccgatcag caggaaggac acgaaaacag ggttgaaagg
     1321 tacgtcgctg ccgcgcaggt tggtcagcca ggcgatgtcg tcaagcgagg aaaccaagtg
     1381 gtaatccgcg cctttttccg ccatcacggc gcgcacgcgg gcgagttttt cggcggcggt
     1441 ttcggagacg taggcggggt cgtggatgaa cacggtttcg gcggggatag cgggacggct
     1501 tgtccacact tggtcaagca gatcgtttgg gtgttcgatg cggatgtttt tggcggtcag
     1561 ggattgcgcc aaagtgcgtt tgccggtgag cgagaccata tcggaaggga tgccgacggc
     1621 ggcgttttcg ggcaggtttg ccgcgagcca ttcgttatac ggcggcactt gcccgctttt
     1681 ttgcagttcg atgccgctgc ccgcaagctg tttggcggct tgttcccaat agcggctgtc
     1741 cacccatacg cccgcttcat cggcggtaac gacgaatgtg ccgaccgagc cggtaaagcc
     1801 cgacaattcg cggcgcgcct gccagtgtgc gggcaggtat tcggacaggt gggggtcggc
     1861 ggaagggatg acgagcgcgt ccaagccttg cgccttcatg gcttcgcgca atgcggacag
     1921 gtaattcgat acggtattca taataattcc tccaatcggg ttttgcggct tcagacggca
     1981 tggacggaaa tctgaaatgc cgtctgaaaa acataaccat cttatcaaat cgccagcctg
     2041 ctgcaaaacg gacgggctga tcggatggtg cgtgtttgcg cgaaaaaagc gatgtaaggt
     2101 tgtttgttgt aaaattttag gcataaaatg tcgtttcatg cctttttttg gaagtaatac
     2161 ggaaaatata gtcaagtttg agcatgaagt tctaatagtg tgtgttggca ggtatttgga
     2221 ttacgttaca atacgcgctt tcatcaaaat ttccctaccg ggcggtatcc tgtttttgaa
     2281 gacggtagga tttgggtttg tttgggggca atcccgccca aattggagca atagataggg
     2341 ctgtgtccga atatgcggct ctgtgtgttg aaacacggtt aaaaatacgg aagactttat
     2401 gtccgaacaa catatttcga cttggaaaag taaaatcaac gcactggggc cggggattat
     2461 gatggcttcg gcggcggtcg gcggttcgca cctgattgcc tcgacgcagg cgggtgcgct
     2521 ttacggctgg cagatcgcgc tcatcatcat cctgaccaac ctcttcaaat acccgttttt
     2581 ccgcttcagc gcgcattaca cgctggacac gggcaagagc ctgattgaag gttatgccga
     2641 gaaaagccgc gtttatttgt gggtattcct gattttgtgc atcctctccg ccacgattaa
     2701 cgcgggcgcg gtcgccattg taaccgccgc catcgtcaaa atggcgattc cctcgctgat
     2761 gtttgatgcc ggcacggttg ccgccttgat tatggcatcc tgcctgatta ttttggtgag
     2821 cggacgttac cgcgctttgg atcgcgtttc caaaatcatc atcgttactt tgagtatcgc
     2881 cacgcttgcc gccgccggca tcgctatgtc gcgcggtatg cagatgcagc ccgattttat
     2941 cgagccgaca ccgtggacgc ttgccggttt gggcttcctg atcgcgctga tgggctggat
     3001 gcccgcgccg attgaaattt ccgccatcaa ttctttgtgg gtaaccgaaa aacaacgcat
     3061 caatccttcc gaataccgcg acgggatttt tgatttcaac gtcggttata tcgccagtgc
     3121 ggttttggct ttggttttcc ttgcactggg tgcgtttgtg caatacggca atggcgaagc
     3181 agtgcagatg gcgggcggca aatatatcgg gcaattgatc aatatgtacg ccgttaccat
     3241 cggcggctgg tcgcgcccgc tggtggcgtt tatcgcgttt gcctgtatgt acggcacgac
     3301 gattaccgtt gtggacggct atgcccgtgc cattgccgaa cccgtgcgcc tgctgcgcgg
     3361 aaaagacaaa acgggcaacg ccgaattctt tgcctggaat atttgggtgg cgggcagcgg
     3421 tttggcggtg attttttggt ttgacggcgt aatggcgaat ctgctcaaat ttgcgatgat
     3481 tgccgctttt gtgtccgccc ctgtgtttgc ctggctgaat taccgtttgg tcaaaggtga
     3541 tgaaaaacac aaactcacat caggtatgaa tgcccttgca ttggcaggct tgatttatct
     3601 gaccggtttt accgttttgt tcttattgaa tttggcggga atgttcaaat gataacgatg
     3661 ccgtctgaaa ccgcaaaccg ctttcagacg gcattgtcgc gtttatggaa aaaaatgccg
     3721 acatcgcgta aaatatgcgc aaattttgtg attcggtcag gtcgtctgaa acagattgcg
     3781 cctgatttat tttttcggaa aaccctatga gcgaacaaaa ccatccgcaa accgagccgc
     3841 agttggacga aaaccaaatc atcgccctgc gccgcgaaaa actgcacaac atccgccaac
     3901 agcgcaacgc ctatcccaac gacttcaaac gcgacagctt cgccgccgat ttgcacgccc
     3961 aatacggcga aatcggcaaa gaagaactcg atccgcaagg cattcccgtc aaagtggccg
     4021 gccgcatgat gctgaagcgt caaatgggca aggcgagctt tgccaccatt caagacgtgt
     4081 ccgggcaaat ccagctttat ctgaacaaca aaggcgtgag ccaagaagtt ttggacgact
     4141 tcaaccattg ggatttgggc gacatcgtcg gcgcggaagg cactttgttc aaaaccaacc
     4201 acggcgaact gaccgtacgc gtgtccgaca tccgcctgtt gtccaaatcc ttgcgtccgc
     4261 tgccggacaa acacaaaggc ttgagcgatc aggaaaccaa ataccgccaa cgctatgtcg
     4321 atttgattgc caacgaagaa tcgcgcaata cctttatcaa acgcagccaa atcatccaat
     4381 ccgtgcgtaa ttttatggtg ggcgagcatt atctcgaagt cgaaaccccg atgatgcacc
     4441 cgattcccgg cggcgcgacg gcaaaaccct tcgttactca tcacaatgcc ttagatattc
     4501 cgctttacct gcgtatcgcg cctgagctgt atttgaaacg cctggttgtc ggcggcttgg
     4561 aacgcgtgtt tgaaatcaac cgcagcttcc gcaacgaagg catgtccgtg cgccacaacc
     4621 ccgaatttac gatgattgaa ttttacgaag ccttttccga ctacgaacgt atgatgcaga
     4681 tggcggaaga catcatccgc aacgcatcgc gcacagtaaa cggtacggca aacatcactt
     4741 acaatggcaa agaagtcgat ttggaaagcc cgttcgagcg tctgaccatc ctcgaagcca
     4801 tcaaaaaata caatccgcac tacaccgacg agcagttgaa cgatgcggaa tggctgaaaa
     4861 aagaaatcgt caaacacggt gaaagcctgc cgccgtcccc gggcatcggc agcctgcaac
     4921 tcgcgctgtt tgaaggttgc gccgagggca agctgtggaa cccgactttc atcatcgatt
     4981 acccggtcga agtttccccg ttggcgcgcg cttcggatac caaacaaggt ctgaccgaac
     5041 gtttcgaatt gttcgttgtc ggccgcgaac tggcaaacgg ctattccgag ttgaacgacc
     5101 ccgaagacca agccgaacgc ttcaaagcgc aagtggcgca aaaagacgcg ggcgacgacg
     5161 aagcgatgca ctacgatgcc gactacatcc gcgcgatgga gttcggtctg ccgccgactg
     5221 gcggttgcgg tatcggcatc gaccgcttgg taatgttgct gaccgattcg caaaccatcc
     5281 gtgacgtgat tctgttcccg cagatgcgcc ccgagtaatc ataaaaacag ttgaaatggc
     5341 aatgccgtct gaacccgatt ggattcagac ggcattttgt atggcaggat tgatagtgaa
     5401 ttaacaaaaa tcaggacaag gcggcgagcc gcagacggta caaatagtac ggaacc
//
LOCUS       DAWEWP010000130         5424 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWEWP010000130 DAWEWP010000000
VERSION     DAWEWP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.130"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1..368)
                     /locus_tag="WGN53_001646"
                     /pseudo
     CDS             complement(1..368)
                     /locus_tag="WGN53_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            complement(453..668)
                     /locus_tag="WGN53_001647"
     CDS             complement(453..668)
                     /locus_tag="WGN53_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140616.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRP"
     gene            complement(956..1309)
                     /locus_tag="WGN53_001648"
     CDS             complement(956..1309)
                     /locus_tag="WGN53_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140617.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            1545..2972
                     /locus_tag="WGN53_001649"
     CDS             1545..2972
                     /locus_tag="WGN53_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ0140618.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3041..4444
                     /locus_tag="WGN53_001650"
     CDS             3041..4444
                     /locus_tag="WGN53_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ0140619.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(4608..>5424)
                     /locus_tag="WGN53_001651"
     CDS             complement(join(4608..5140,5140..>5424))
                     /locus_tag="WGN53_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ0140620.1"
                     /translation="PRYYEQCKNISQTAATFNLSRNTLYLWIRLKKQTGSLKHQVTGL
                     NAVKSDRQKPAQYIGQHQDAYLHEIAKHFDCTAATICYALKQMGITRKKRPTTYKEQD
                     PAKVTHYLTQPAEFSDYQRVYLDETGFDRYLFRPYARSLKGQIVKAQISGKRYQRLSL
                     VSAQVGNRLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREM
                     AEKLGHKVLPLAPYSPELNPIEKVWANIKQYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1368 a         1311 c         1397 g         1348 t
ORIGIN      
        1 gaaaccgaaa acttcttcgg cgagcatgga cgcggcgcga ataaaatctt cgtcttccaa
       61 aagacttaca cgaacgccgt atttatcgaa aaaggttttt tcatgaaatg aaaagctaat
      121 ttgatcaatg aacgccgaat cagatacacc gcgccgaaga ggaacgccta acaggtttcc
      181 tttaccgtcc gttatgtacg tttcgtaaca ttcgaagact tcctgaaccc tgcctgccgt
      241 ttcggtttct gtccccccct gttagataag gggggaagat ttgaagcggt tgccggcttc
      301 ctgccgtccg ctggcgcgtc cgtcatcacg ccggcaaccg cctttgtcat cccttgctta
      361 tcttccatgg tgcgaatcct caaaaacggg caaaaaaaag ccctgttact tgtagaaagt
      421 aaaggacgtt aatttttgtt aatcgtccct tcttagggac gcaatatata aggttttaaa
      481 acgctaagaa caaaatccga taacggctct gaagaagtaa gtctttcttt attgcacaag
      541 atagcaaact tccacggcat tctcgcccca cccgtgccgt ccaagccttt ttcaaagaca
      601 tagatggttt tcttggcaat cacttcgtca gtttggcggt cgataatccg tatggtcgca
      661 cctgccaccc agtgcctgcg cagcttggaa tcgacattgt tttcgaacgt taccgcataa
      721 cgggcggggt gtatatgatt aaatatttga tttatcggaa aatctttacc tgaataccga
      781 ataatatcgg aatggttggg ttgcaggaca tccacatagc ggtattcccc atcgctaaaa
      841 aatcctagga aacgagcaat aaaatttacg ccttcgctgg tctgtaaagc cgcattgtcc
      901 cacatcgggt ctctggtttt tgcatctgcc gaaacggtac gctcaggtac taccttcaac
      961 agcataatcc cttccacatt gtccgccgtc tggtaaatct ttcccccgcc gttttgcatt
     1021 gttcgttaaa cacggcttcg gcttctttgt attttctgtc ccactcttct tgtgcctgta
     1081 tttcttcttt gatcgggccg aattgtttgg gaataatcca aacaaacagc atcaggatag
     1141 cggcggcggt caggctgcct gaaaggattt tgccggggtt ccgtttgggc tttttatagg
     1201 caaagcggac gagaaaccaa agcaacagca gcatggtgcc ccaatagccg attgagaata
     1261 ggatggccaa accttctagg aaatggcgta aatcgtttgt ggtaaacatg ggttgttcct
     1321 gtggttaaat gtgcaggctg ctttttgccg aaccttgccg catctcaaaa gcagcctgcg
     1381 cttcagcgtt gcgttacgca gtaaaataat gaatatttgt aacgacttgg gtattttttg
     1441 tcaatattcc cgcctttccc ttaacagctg ccgcgctttc cgttaaaatt cctttacata
     1501 tttatattgt ttcctgtttc tatattgcca aggttatacc cgttatgttt ttctccgccc
     1561 tgaaatcctt tctttctcga tacattactg tatggcgcaa tgtttgggcg gtgcgcgacc
     1621 agttggaacc gcccaaacgc acggcggaag aacaggcgtt tttgcccgcg catttggaac
     1681 tgaccgatac gccggtctct gccgctccga aatgggcggc gcgttttatt atggcgtttg
     1741 cgcttttggc tttgttgtgg tcctggttcg gcaaaatcga tattgtggcg gcggcttcgg
     1801 gcaaaacggt gtcgggcggg cgcagcaaaa ccatccagcc gctggaaacg gcggtggtta
     1861 aggcggtaca tgtgcgcgac gggcagcatg tgaaacaggg agaaacgctg gcggaactgg
     1921 aggctgtggg aacagacagc gatgtggtgc agtcggagca ggctttgcag gctgcccaat
     1981 tgtccaaact gcgttatgaa gcggtattgg cggcattgga aagccgtacc gtgccgcata
     2041 tcgatatggc gcaagcacgg tctttaggtc tctccgatgc cgatgtgcaa tcggcgcagg
     2101 tgttggcgca gcaccagtat caggcatggg cggcgcagga tgcgcaattg cagtcggctt
     2161 tgcgcggcca tcaggcggaa ttgcagtcgg ccaaggcgca ggagcagaag ctggtttcgg
     2221 tgggggcgat cgagcagcag aaaacagcag actaccgccg tttgcgggcc gacaatttta
     2281 tttcggaaca tgcgtttttg gagcagcaga gcaaatcggt cagcaattgg aacgatttgg
     2341 aaagtacgcg cggtcagatg aggcagattc aggcggccat tgcacaggcg gagcagagtc
     2401 gggtgctgaa tacgcagaac ctgaaacgcg atacgctgga tgcgctgcgc caggcaaacg
     2461 aacagattga ccaataccgc ggccaaacgg ataaggcaaa gcagcggcag cagctgatga
     2521 caatacagtc gcctgcggac ggcacggtgc aggaattggc cacctatacg gtgggcggtg
     2581 tggtgcaggc tgcccaaaaa atgatggtgg ttgcgcccga tgacgacaaa atggacgtgg
     2641 aagttttggt attgaacaaa gacatcggtt ttgtggaaca gggacaggat gcggtggtga
     2701 agattgagag ttttccctat acgcgctacg gttatctgac gggcaaggtg aaaagtgtca
     2761 gccatgatgc ggtaagccac gaacagttgg gcttggttta tacggcggtg gtgtcgctgg
     2821 acaaacatac cttgaatatt gacggcaaag cagtgaatct gacggcgggc atgaatgtca
     2881 cggcggagat taaaacgggt aaacggcggg tgctggatta tctgttaagc ccgctgcaaa
     2941 ccaaattgga cgaaagcttt agggagcgat aggcggatcc gtactgggca tttgttatcc
     3001 gccggttcgg acatgcagac tgcctgaaac cattgcccgg atgacattgc tcaatctaat
     3061 gataatgcaa gattacggta tttccgtttg cctgacactg acgccctatt tgcaacatga
     3121 actattttcg gctatgaaat cctatttttc caaatatatc ctacccgttt cactttttac
     3181 cttgccacta tccctttccc catccgtttc ggcttttacg ctgcctgaag catggcgggc
     3241 ggcgcagcaa cattcggctg attttcaagc gtcccattac cagcgtgatg cagtgcgcgc
     3301 acggcaacaa caagccaagg ccgcattcct tccccatgta tccgccaatg ccagctacca
     3361 gcgccagccg ccatcgattt cttccacccg cgaaacacag ggatggagcg tgcaggtggg
     3421 acaaacctta tttgacgctg ccaaatttgc acaataccgc caaagcaggt tcgatacgca
     3481 ggctgcagaa cagcgtttcg atgcggcacg cgaagaattg ctgttgaaag ttgccgaaag
     3541 ttatttcaac gttttactca gccgagacac cgttgccgcc catgcggcgg aaaaagaggc
     3601 ttatgcccag caggtaaggc aggcgcaggc tttattcaat aaaggtgctg ccaccgcgct
     3661 ggatattcac gaagccaaag ccggttacga caatgccctg gcccaagaaa tcgccgtatt
     3721 ggctgagaaa caaacctatg aaaaccagtt gaacgactac accgacctgg atagcaaaca
     3781 aatcgaggcc atagataccg ccaacctgtt ggcacgctat ctgcccaagc tggaacgtta
     3841 cagtctggat gaatggcagc gcattgcctt atccaacaat catgaatacc ggatgcagca
     3901 gcttgccctg caaagcagcg gacaggcgct tcgggcagca cagaacagcc gctatcccac
     3961 cgtttctgcc catgtcggct atcagaataa cctctacact tcatctgcgc agaataatga
     4021 ctaccactat cggggcaaag ggatgagcgt cggcgtacag ttgaatttgc cgctttatac
     4081 cggcggagaa ttgtcgggca aaatccatga agccgaagcg caatacgggg ccgccgaagc
     4141 acagctgacc gcaaccgagc ggcacatcaa actcgccgta cgccaggctt ataccgaaag
     4201 cggtgcggcg cgttaccaaa tcatggcgca agaacgggtt ttggaaagca gccgtttgaa
     4261 actgaaatcg accgaaaccg gccaacaata cggcatccgc aaccggctgg aagtaatacg
     4321 ggcgcggcag gaagtcgccc aagcagaaca gaaactggct caagcacggt ataaattcat
     4381 gctggcttat ttgcgcttgg tgaaagagag cgggttaggg ttggaaacgg tatttgcgga
     4441 ataaagcagg ctgaaacggt tatgaaattc ccaaagcagc ctgcaccccg tttcgaaagt
     4501 gcaggctgct ttgggattga tccgatactt tcacattctc attgccttta aataaagcac
     4561 agttaaaaac acatttaaat taagtgcgaa ttaattcaaa tgtatattca attaaaatca
     4621 aaataggaca gtagtgcatc gtcaaatcgg gcgtaatcag acaaaacggt tcgcagatac
     4681 tgcttaatat tcgcccacac tttctcaatc gggttgagct caggtgaata aggtgcaaga
     4741 ggcaatacct tatgtcccaa tttttccgcc atttcccgta agacacccat acggtgaaat
     4801 cgcgcattat ctaaaataat caccgatttt tgagtcaatg cgggcagtag gcattgctga
     4861 aaccacgctt caaaaaagac tccggtcatc gtattttgat aaaccatcgg agcaatcagc
     4921 cggttgccga cttgtgcgga caccaaagat aagcgttggt atctttttcc acttatctgc
     4981 gctttcacta tttgcccttt caggctgcgg gcatagggac ggaacaggta gcggtcaaat
     5041 cctgtttcat ccaaataaac acgttggtag tcggaaaatt cggccggctg tgtcaaataa
     5101 tgcgttactt tggccgggtc ttgttctttg taagtggtgg tctttttttg cgcgttatcc
     5161 ccatctgttt gagtgcatag caaatggtgg ctgccgtaca atcaaaatgt ttggcgattt
     5221 catgcagata ggcatcctgg tgttgcccaa tatattgagc cggtttttgc ctatccgatt
     5281 tgacggcatt tagaccggta acttgatgtt ttaggctgcc tgtttgtttt ttaaggcgaa
     5341 tccacaggta aagcgtgttt cttgacaagt taaacgttgc tgcggtttgg ctgatgtttt
     5401 tgcattgttc gtaatatcgt ggat
//
LOCUS       DAWEWP010000131         5386 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWEWP010000131 DAWEWP010000000
VERSION     DAWEWP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.131"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(103..2934)
                     /gene="lbpA"
                     /locus_tag="WGN53_001652"
                     /gene_synonym="tbpA"
     CDS             complement(103..2934)
                     /gene="lbpA"
                     /locus_tag="WGN53_001652"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ0140621.1"
                     /translation="MNKKHGFQLTLTALAVAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETHPHGDIADGVAYGINRLDAFRQTYGIKKPSEGGEYFLAEGESEL
                     KPVAKVAGNGNYLNNQLNRWVKERIEQNQPLSAEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAKKF
                     RDKSGVYDGDDFRDGLYFVPNIEEWKGDQKLIRGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISEYS
                     DYTDKGDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2931..5114)
                     /locus_tag="WGN53_001653"
     CDS             complement(2931..5114)
                     /locus_tag="WGN53_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0140622.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAKPSIEIMPVNRPAVGAAMRLPRRNTAFHREDGTEIPNSKQAEEKLSFQEG
                     DVLFLYGSKGNKLQQLKSEIHKRDSDVEIRTSEKENKKYDYKFVDAGYVYVKGKDEIK
                     WTSDYKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDAKRHRAGKAVGI
                     DNLGYYTFYGNDVGATSYAAKDVDEREKHPAKYTVDFGNKTLTGELIKNQYVKPSEKQ
                     KPLTIYNITADLNGNRFTGSAKVNPDLAKSHANKEHLFFHADADQRLEGGFFGDKGEE
                     LAGRFISNDNSVFGVFAGKQNSPVPSGKHTKILDSLKISVDEASGENPRPFAISPMPD
                     FGHPDKLLVEGHEIPLVSQEKTIELADGRKMTVSACCDFLTYVKLGRIKTERPAAKPK
                     AQDEEDSDIDNGEESEDEIGDEEEGTEDAAAGDEGSEEDEATENEDGEEDEAEEPEEE
                     SSAEGNGSSNAILPVPEASKGRDIDLFLKGIRTAETNIPQTGEARYTGTWEARIGKPI
                     QWDNHADKEAAKAVFTVDFGKKSISGTLTEKNGVEPAFRIENGVIEGNGFHATARTRD
                     DGIDLSGQGSTKPQIFKANDLRVEGGFYGPKAEELGGIIFNNDGKSLGITEGTENKVE
                     ADVDVDVDVDVDVDADADVEQLKPEVKPQFGVVFGAKKDNKEVEK"
BASE COUNT         1176 a         1452 c         1232 g         1526 t
ORIGIN      
        1 ggaaatcttg tttatatcga atcaaaaaaa cctgcacctt aatcagttgg cggtttagtc
       61 cgacttttgg ggtgcagatc aagctttcag acggtatttc ctttaaaact tcatttcgag
      121 cgcgagactg aagttcctgc ccggtgcggc ataccttcca tagttgctgt cgccgccgtg
      181 ccggtttgcc gtgctttccg cggtctggcg caaggattcc caagtaacgt agcggtagtt
      241 gccgatattg tagatagccg ccctcaaggt cagccgtttt ttcagattca gataggcgga
      301 aacgtctgcc gtcgaccaag aagacgacgc tctttttgtc gaatatcgtt tttgatcgcc
      361 tgccagataa gcaagctcgt cagggttttt ccctttggaa taggtcagca taatgtttgc
      421 gccccatttc ccctcgggct ggtcgtatcc gaaccccaaa acataacgcg acggctgtac
      481 cgcatccaaa gcatagctgc ggagagacag tcccggccgg ttggataccg atttcggttt
      541 gatacggttg tacgccaaag tcgtgtacaa accttcgggc agtttgccgt acacaccgtt
      601 ccagtcgatt tttcccaata tattaacgcc ttggagcgac atattttggg cattgtaata
      661 atcgcgtata tcaatctctg tcaattgtcc tgcctgattc ggcaatttgg ttttgtgatc
      721 ggcaacggca atcatatcgg tataacggtt gcggaagctg ctgatttcca aaaagccgaa
      781 atcgcccttc cactgcaaac cgatttcgcg gttggctgcc ttttccgatt tcagggcggg
      841 acgctgccag cctttcggat aatcgtgata aatgtctatc ccgaaaagtt cttggaatga
      901 gggcgttctg aagccgctgg aggcgcggta agacaaagaa aaatgccggt tcggtttgaa
      961 cacgatgccg ctgttccacg aacggtcaac ataccgcccg ctgcggacga gttcttccga
     1021 cgtggtgaag tttttccggt cgtacctgcc gcccaagctg aaatcgaaat atttgccgat
     1081 tgaaaaacgg tcgttcaaag aaatgtggat attgctgccg ttgatttttc ttggcacgca
     1141 tttgcgggaa cgcagggttt cgatgtagcc gcagaccgac ccttcgacga cttcgggctt
     1201 acccaaaaga tacttgtctc ccttatcggt gtaatccgag tattccgaaa tccttgccgc
     1261 attgtgagaa agctgttcgg ggcgggaaat cgcgttggaa gcatcgtaac cgaagcccaa
     1321 agtcagatgg tgtttcgtcc atttgttttt cagcgatttc tcaaacgagg cattcaaaac
     1381 attgtgctgt tcgcggtagt ggaaacggtc gctgctgtcg taggaatacg gtttgtccgc
     1441 cgacgcgcgg caggatttgt ccacggcagg atacacggcg caattcagct tcagcgtgtt
     1501 gttgtcggtt gccacgccct gtttgtcaaa cgacaacacc gccttatccg cccagttatc
     1561 cgagtatttc tcattttcat aacggtacag caaacccata cggcggcggc ggtgatgttc
     1621 gtcaataaat ttggtgcggg aatatttcaa acctatgccc ctaatcaact tttgatcgcc
     1681 cttccactct tctatattcg gcacaaaata caagccgtcg cggaaatcgt cgccgtcgta
     1741 caccccgctc ttgtctctaa actttttcgc ctcgtccgta ccgtaatact gtttttccgt
     1801 catatcgcgg atatcgtaac gctgtttggt atcctcaaac acgccgccga cataatgcct
     1861 gccgccgaag cggtagccca gcttggcaag ccacgaaccg ctgcggtaat ccatcggatc
     1921 gggcaatatc ctgccgccgc ccgtgtaagc ttgggcggac aggttttcgt ggcgcgcctg
     1981 cgcctcccgc accatcgctt cttcttcagc acttaaaggc tgattttgtt caatacgttc
     2041 ttttacccag cggttgagct ggttgttcaa atagtttccg ttgcctgcca cttttgccac
     2101 aggcttgagc tcactctcgc cctccgccag gaaatattcc cctccttcac ttggtttttt
     2161 aatgccgtat gtctggcgga acgcgtccaa acggtttatg ccgtatgcca ccccatccgc
     2221 aatatcgccg tgcgggtgcg tttcccgccc ctgacgttcg gttcggatta gcagcccttc
     2281 ccaaccgtct ttactgaaac ccgcgccgag cgacttcata aattggcggt ttttactgcc
     2341 gtaggcggtt tttgcctgta tcccccagct tttgccgtct gaaatcaggt ctgccgcctc
     2401 tttggtgcgg aaagcgaccg cgccgccgag tgcgccgctg ccgtgatcgg acgaaccggc
     2461 acctttgtcg atttccaccg tgctgatgtt ttcatattcg atttcgttga ttgcaccgct
     2521 gccgccgcgt ccgccgtatc cgctcaacga tccctgcacg gtaaacgcct gtatttgggc
     2581 aacgccgtca accgaaaccg ccacacggtt tttatccacg ccgcgtatcg agtagccgcc
     2641 gctcgcgccg ttgccctgtt cgacaaccgc cacgcccgga tcgtagcgcg tcaggtcgcg
     2701 gataccgagt acctgttctt tgttcaacgt ttccgacgtt ttgacgattt tgcccaaacc
     2761 ggtcgcctct ttcgatcgcc gtcccacttt ggcagcacgg acggtaacct ctttcaggga
     2821 ttgggtctgc gccgcatcag gtgccgccgt ttcagggttt gccgcatatg acgggaaggc
     2881 ggcggcaaca gccaaggcag tcagggtaag ctgaaaaccg tgtttcttat tcatttttcc
     2941 acctctttat tatctttctt cgcaccgaat accacgccga attggggttt aacttcaggt
     3001 tttaactgtt caacatcagc atcagcatca acatcaacat caacatcaac atcaacatca
     3061 acatcagctt caactttatt ttcagtacct tcagttatac caagagattt cccatcatta
     3121 ttgaaaataa taccgcccaa ttcctccgcc ttcgggccgt aaaatcctcc ttctacacga
     3181 agatcattag ctttgaagat ctgcggtttg gtcgaaccct gcccggaaag gtcgatgccg
     3241 tcatcccgag tgcgcgctgt cgcatggaaa ccgttgccct caatcacgcc gttttcaata
     3301 cggaaagcag gttctacacc gtttttctcc gtcagcgttc cggaaatcga tttcttgccg
     3361 aaatcaacgg taaatactgc ttttgccgct tctttatccg catgattgtc ccattgaatg
     3421 ggtttgccga tacgcgcttc ccaagtgccg gtatagcgtg cttctccagt ttgcggaata
     3481 ttcgtttctg ccgtgcggat acctttcagg aaaaggtcga tatccctgcc tttagaggct
     3541 tccgggacag gcaggatggc gtttgaactg ccgttgcctt ctgccgacga ttcttcttca
     3601 ggttcttcag cttcgtcttc ttcgccgtct tcgttttctg tggcttcgtc ttcttcgctg
     3661 ccttcatctc ctgcggctgc atcttcggtg ccttcttctt catcgccgat ttcgtcttcg
     3721 ctttcttcgc cattatcaat gtccgaatcc tcttcgtcct gcgccttcgg tttggcggcg
     3781 gggcgttcgg tttttatccg tccgagtttc acataggtca aaaagtcgca acaagcactg
     3841 acggtcattt tcctgccgtc ggcaagctcg atggttttct cttggctaac caaaggaatt
     3901 tcatgccctt cgacaagaag tttgtcggga tgaccaaaat cgggcatagg agaaatggca
     3961 aacggtcggg gattttcacc acttgcctca tcaacggaaa ttttcagaga atccaagatt
     4021 ttggtgtgtt ttccagacgg cacggggcta ttttgtttgc ctgcgaatac accgaatacg
     4081 ctgttgtcgt tgctgataaa ccgtccggca agctcttccc ccttatcgcc gaaaaaaccg
     4141 ccctcaagcc gctgatcggc atcggcatgg aaaaacaaat gctccttatt ggcatggctt
     4201 ttcgctaaat caggattgac cttggcactg ccggtaaagc ggttgccgtt taaatcggca
     4261 gtgatgttgt aaatggtcag cggtttttgc ttctcactgg gtttgacata ttggttttta
     4321 atcagctcgc ccgtcagggt tttgttaccg aaatctaccg tatatttagc aggatgtttt
     4381 tccctttcgt cgacatcctt agccgcataa gaagttgcac caacatcgtt accataaaat
     4441 gtgtaataac ccaaattgtc aatgccaacc gccttacctg ctcgatgacg tttggcatcg
     4501 gtcatatatt gccagttacc agaatattcc accgttcccg cactcggtaa agattgggaa
     4561 ggacgttctc cggaataata tacaaaaccg tcataaccta agcggttgga aaactgcttg
     4621 taatctgaag tccacttaat ttcatctttt ccctttacat atacataacc tgcatctaca
     4681 aatttataat catatttttt attttccttt tctgatgtcc taatttctac atcggaatca
     4741 cgtttatgaa tttcgctttt aagttgttga agtttatttc cttttgaacc gtataaaaac
     4801 agaacatcac cttcttgaaa cgacagcttt tcttctgctt gtttgctatt tggaatttcc
     4861 gtgccatctt cacgatgaaa agcagtattc cgccttggca gccgcattgc cgcaccgacg
     4921 gcgggccggt tgaccggcat gatttctata gaaggtttgg cagggggcgg agtgggaacg
     4981 tccttagact tgaaagtgac ggggtacgcg gtcggcgttg attcgacaac aggctgcacg
     5041 ccgaaattgc cgccgataca agatgccaaa agtaagggca acaagacaat gccgccataa
     5101 ttcggtttac acatccctac ttttcctcct atttgattaa taataattat cattatatta
     5161 atatgtacag ataatatcaa cccgttttta tagtgaatta acaaaaatca ggacaaggcg
     5221 acgagccgca gacagtacag atacattccg tcattcccac gaacctacat tccgtcattc
     5281 ccacgaacct gcaccacgtc attcccacga aagtgggaat ccagttcgtt cggtttcgct
     5341 tgttttaagt ttcgggtaac ttctacttcg tcattcccac gaacct
//
LOCUS       DAWEWP010000132         5321 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWEWP010000132 DAWEWP010000000
VERSION     DAWEWP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.132"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            295..1728
                     /locus_tag="WGN53_001654"
     CDS             295..1728
                     /locus_tag="WGN53_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ0140623.1"
                     /translation="MNEYSQLIKHPDISLSPISDSIGIGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALASLAYEAGIPQDLLPVVSGSASEIGHEFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSAIYDEFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGASCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1837..2625
                     /locus_tag="WGN53_001655"
     CDS             1837..2625
                     /locus_tag="WGN53_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ0140624.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDQWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            2993..4549
                     /locus_tag="WGN53_001656"
     CDS             2993..4549
                     /locus_tag="WGN53_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0140625.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4566..5312
                     /gene="surE"
                     /locus_tag="WGN53_001657"
     CDS             4566..5312
                     /gene="surE"
                     /locus_tag="WGN53_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ0140626.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKNPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         1248 a         1341 c         1515 g         1217 t
ORIGIN      
        1 gtttggattt ttgccatact ctaccacacg ttgcaactgc aatctttgtt ccttatatat
       61 tatcaaaggg ctgcattact tttctttcta aattaccatt gtataaaaag tatttttctt
      121 aaacgacagg gattgcgccc gcgccatatt cagacaccgc ccggatgttg cactgcccga
      181 tcggatgctt cagacggcat cggaagggtt tgcagttttg gaatatgaag atgataatgt
      241 ccgcgagatt gacggcattt gaaagcggtt attccgcata ccggaggata cgaaatgaac
      301 gaatattccc aattaatcaa gcatcccgat atttcccttt ccccgatttc agacagcatc
      361 gggatcggca atccggcgac gggtgaaatt ttggcgtatg tccgcaacac gggttcggac
      421 aaactgaaaa acctgattca aaaggcggcg gcagcgcaaa aattatgggc ggcgaaaact
      481 gctttggaac gcgccgacat attgtggcgt tggtattttt tagttaaaga aaacaaagaa
      541 gcactggcgc gcctgatgac gatggagcag ggcaaaagcc tgaccgaggc gcgcggcgag
      601 attgattatg cggcttcgtt tgtgcgctgg tttgcggaag aggcgcggcg gattgacggc
      661 gatgtgttaa cgagtgtaaa agcgtcgcaa aaactggttg tgttgaaaca gcctgtcggc
      721 gttaccgccg cgattacgcc gtggaacttc ccatctgcga tgattgcgcg caaggccgcg
      781 cctgctttgg cggtgggttg cgcgatgatc gtcaaacccg catcgctcac gcctttgagt
      841 gcgtatgcct tggcttcgtt ggcttacgaa gcgggcatac cgcaggattt gttgcctgtt
      901 gtcagcggca gtgcttcgga aatcggccat gaatttgcta cgaacccgat tatccgcaaa
      961 atcagcttca ccggctcgac cgaagtcggc gcaaaaattt ttgccgacag cgcggcggac
     1021 attaaaaaac tcagtttgga gctgggcggc aacgcgccgt ttatcgtgtt tgacgatgcc
     1081 gatttggaca aagccgtcga aggcgcgctc gccagcaagt tccgtaacag cggtcagacc
     1141 tgcgtctgca ccaaccgcgt ttacgctcaa tccgccattt acgacgaatt ttgccgcaaa
     1201 ttgagtgaaa aagcagccgc gctcaaattg ggcaacggct tggaggatgg tgtgaaccaa
     1261 gggccgctga ttgaggaaaa agcggtggag aaagtcgagc agcacatcgc cgacgcgctt
     1321 gctaaaggtg caagctgcct gaccggcggc aaacgcagcg cgttgggcgg aacgtttttc
     1381 gaaccgactg ttttaagcgg cgtaacggcg caaatggcgg tggcacgcga agaaaccttc
     1441 gggccgttgt gtccggtatt ccgttttgaa accgaagccg aggtcatcga ggctgcgaac
     1501 aatacggaat acggtttggc agcttacctt ttcaccgccg acaccgcccg ccaatggcgc
     1561 gtcggcgaag ccttggaata cggtatggtc ggcatcaata cgggcttaat cagcaatgaa
     1621 gcggcaccgt tcggcggcgt gaaacgtagc ggtttgggac gtgaaggcag caaatacggt
     1681 gcggacgaat atctagaatt gaaatatctg tgtatggatg tcgggtgacg gatgccgtct
     1741 gaacggcagg atttcagacg gcgccgcatt ttaagcagtc tctatctgtt gtacaatgcg
     1801 ccctgttttt acggttattt ttgatttgaa caagatatga tggaaaacgg aaaaacagtc
     1861 ccgagatggc gttttgcctt gaaaagtgcg ggctggcacc tcttaatcag cctgtcggtt
     1921 gcagggctgg cggcattgct ggtttttaag gtttggtatc cttatcctta tgccgagctg
     1981 acgggagggc tgtcgcttta tcagctggtg gtggctgtcg atattgtatg tggtccgctg
     2041 ctgactttaa ttttggcaag cccgaagaaa aagacaaagg cacgcatggt cgatttttcc
     2101 atggtcggca tcatccagct ggcggctttg gtgtacggtc tgcacagcgt ttcgctggcg
     2161 cgtcctgtgg tggaagcgtt tgaacaggat cgtatgacca ttgttacggc ggcggaagtc
     2221 gtggtcgaag atttgcacaa agcccccgaa gggctgcaaa gcctgtcgtg gttcggcatc
     2281 cgccgcattg cattgaaaga acctgaggat gcggatgaga agaacaagac gctggatttg
     2341 tccctgaaag gtatcgagcc gagtatgcgt cccgaccagt ggctgccgta ttccgacaag
     2401 gaagcagaag aaatccgcaa acatctgaaa ccgctgaaag tcttggcgga tgcgagaaaa
     2461 acgacggttg cggacattct gaaacaggca ggtctcgccg aaggggagga gctgtattac
     2521 ctgccgttta ccagcagcag gcagaaagag tggatagtca ttaccgataa agagggcaac
     2581 accaaaggct acgcgccgat agacggcttc atcatcaccc cttaagcgtt gggactccgt
     2641 ccgcactcga acatccgttc ttcgcggcgg tagaatcaga ctgtatttga gaggggcgca
     2701 acccctcaat cagggcaaca cataggggaa ggctttatgt gccgtccgtg tgttgaaaca
     2761 tttcaaaata agctcatgcc gtccgaacat cctttcagac ggtatggcat tttgccattg
     2821 ccccgataag ctgttaaact atttaaatta tttcgaccga aggtacacgc acatgcaaga
     2881 ccccgagcag agcagcaagc ccgcccgccg ttttttgtgc gtaccgtctg ttcaggcagc
     2941 ttgagcgctt caggctgcct tttcgcgttt gaacttgagg aatacgaaaa tcatggattt
     3001 cagttggttg gcagaaccgc atacctggat aggttttgcc acgcttttgg tgttggaagt
     3061 tgtattgggg atagacaatc ttgtctttgt ggcgattttg gcaaacaagg tccagcccgc
     3121 acggcgcgac cgcgcacgga ttatcgggct ggggctggca gtcgtcatcc gcatcattat
     3181 gcttgctttt atggcgcaca tcatcacgct gaccgagccg ctgttccaaa tcggtggcct
     3241 cgccgtttcc ggcaaggaca tgattatgct cgcgggcggt attttcctgc tttacaaagc
     3301 caccaccgaa ctgcatgaac gcctcgaagg gcacaaccgt tttaccgttg ccgacaacca
     3361 aaaaaaacac gcgccgtttt ggggcgtggt cgcgcaaatc ctgatactgg atgccgtgtt
     3421 ttccatcgat tcggtcatta ctgcggtggc gatggtcgat catatcgtcg tggcgatggg
     3481 tgcggtcgtc gtcgcgatgg ctgtaatgat ttctgccagc aaactcttga ccgaatttgt
     3541 cgacagacac cctaccgtcg tgatgctctg ccttggtttt ttgttgatga tcggtttcag
     3601 cctgattgcc gaagccttcc atttccacat tcccaaaggc tacctctacg ccgccatcgg
     3661 cttctcgatt ttaatcgaat tgtttaacca gatttcgcag cgcaacagcc gcaaaaacga
     3721 ctacatcggc agctcgtggc gcaagcgcac cgccgaaaac gtcttgggta tgatgggtat
     3781 acgcgaaagc gtgcttgccg acgcgggcgg cgaatccggg gacgacgcgc attttgaaga
     3841 aaacgaaaaa tcgatgatac gcagcgtgct gacgcttgcc gaacgcccga ttatgggggt
     3901 gatgatccca cgccgcgaca tcgaacggct ggacatttcc caaagccgcg aagaacagtg
     3961 tgcccaactg caaaacacgc cttacagccg cctgctcgtt gtcggtaagg cgggcgtgga
     4021 cgaacctttg ggctacatca acaaaaaaga cctgctgtcc caactgctgg aaacaggcgg
     4081 tctcgacatt cagacggcat tgcgccagcc actcgtcctg cccgacagca ccaccgcgct
     4141 gggcgcaatc gaactcttcc gccaaagcag cgcggattat gctttggtgg tggacgagtt
     4201 cggcgcggta ttgggcatgg taaccatgaa agacctgctc gaaaccatcg caggcgagtt
     4261 ccccgaagaa tttgagcgcg aagaagaacc agccgttcag gggaatcccg atgaaagcct
     4321 gacggtggaa ggcgcgttgg aatatgtgga actcgcaccg caactcaacc tgccgcagca
     4381 ggaggaagat gccgatttcc atacggttgc cgggctgatt atggaagaat tgcaaaccat
     4441 ccccgatgtc ggcgattttg ccgatttcca cggctggcgg tttgaagtgg tcgaaaaaga
     4501 agggcagcgc atcgagcggg tcaaaatcac caaattgccc gaagaataag cattcaggat
     4561 agaaaatgaa cgttttgatt tccaacgacg acggctacct ctccgaaggt attgccgttt
     4621 tggcgcgcgt tacggcggaa tttgccaacg tcagggtggt tgcgcccgaa cgcgacagga
     4681 gcggggtcag caattcgctg acgctggaac gccctttgca gttgaaacag gcgcaaaacg
     4741 ggttctacta tgtcaacggc acgccgaccg actgtatcca catcgggcag tctgtatttt
     4801 cggattttca ggccgatttt gtcttttcgg gcatcaaccg gggcgcgaat atgggggacg
     4861 acacgcttta ttcggggacg gttgcggcgg caaccgaagc ctaccttatg ggcatacccg
     4921 ctgtggcgtt ttccttaaac gacgcttccg gacgctattg ggcaaccgca gaaaaggcac
     4981 tgtggacatt gttggcgcat tttttcaaaa accccccgca gtcccctatt ttgtggaaca
     5041 tcaatatccc cgccgtcgag ccggaagatg tgcggggcat taaaatcgcc cgtttgggca
     5101 ggaggcatca cggtcagaac gtcatccccg cgcgcaatcc gcgcggcgaa cagatttatt
     5161 ggataggccc ggtaggcagg gtttccgaca aagaagaggg aacggatttc ggtgaatgcg
     5221 gcgcaggttt cattaccgta acgccgctgc aaatcgacct gaccgcctat ccggacatgg
     5281 cggaaacagc ggcgttctgg catgcggact gaccgtttca t
//
LOCUS       DAWEWP010000133         5255 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWEWP010000133 DAWEWP010000000
VERSION     DAWEWP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.133"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            109..804
                     /gene="pgl"
                     /locus_tag="WGN53_001658"
     CDS             109..804
                     /gene="pgl"
                     /locus_tag="WGN53_001658"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ0140627.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            785..1771
                     /locus_tag="WGN53_001659"
     CDS             785..1771
                     /locus_tag="WGN53_001659"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ0140628.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1821..2669
                     /gene="hexR"
                     /locus_tag="WGN53_001660"
     CDS             1821..2669
                     /gene="hexR"
                     /locus_tag="WGN53_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ0140629.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2790..4436
                     /gene="pgi"
                     /locus_tag="WGN53_001661"
     CDS             2790..4436
                     /gene="pgi"
                     /locus_tag="WGN53_001661"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0140630.1"
                     /translation="MKHFHDLPAWPKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNCT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDESAVAKHFVAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPAILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLIRLYLQTNRK"
     gene            4450..5001
                     /locus_tag="WGN53_001662"
     CDS             4450..5001
                     /locus_tag="WGN53_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0140631.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHVCSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSELACKTAETEARLEELH
                     NRKKALIDEMAREADKKELPKRL"
BASE COUNT         1335 a         1464 c         1391 g         1065 t
ORIGIN      
        1 cagtcccgat atttggtatc aaaagcataa acaactgttc ggccgacata ttgctcaacc
       61 cgtttccata acggaatatg ccgtctgaaa taaaaaaagg acacaaatat gtttgtttgg
      121 cacgaatacg aaaatgcggc agaagcggcg cagtctttgg ctgacgcagt ggcggatgct
      181 ttgcagggcg cactggacga gaagggcggt gcggtgttgg cagtttccgg cggacgttcg
      241 ccgattgcat ttttcaacgc cctgtcgcaa aaagatttgg attggaaaaa cgtcggcatc
      301 accttggcag atgaacgcat cgtgccgacc gtccacgccg acagcaatac cggtttggtg
      361 cgcgaatacc tgttgaagaa caaagcggaa gcggcaatgt ggattcctat ggtggaagac
      421 ggaaaaactg aaaccgaatt acatcccgat gctgttgtcg attatgcact gaaacattac
      481 aaacagcccg atgttttggt tttgggtatg ggaaacgacg ggcatacggc ttcgattttc
      541 ccgaaagctc cgcagtttca gacggcaatc gacggttcgg caggtgtcgc gctggtgcat
      601 accacgcccg ttaccgcgcc gcacgagcgc gtcagtatga ccttggatgc gattgcccat
      661 acggggcatg tgtttttggc gatacggggc gaagagaaaa aagccgtgtt cgaccaagcc
      721 gcacaaggcg aaaaccgcga atatccgatc aacctcgttt tgaaccatca aggagtgaac
      781 tgccatgtct tctacgccga ataaacaagc cggatatccc cgactggtcg ccgatatcgg
      841 cgggacgaat gcacgctttg cgctggaaac cgcgccgcgc gtcattgaaa aagccgccgt
      901 gcttccgtgt aaagactacg atacggttac cgatgcggtg cgtgcctatc tgaatcaaag
      961 cggtgcaaca gccgtacggc acgcggcatt tgccatcgcc aacccgattt tgggcgactg
     1021 ggtgcagatg accaaccacc attgggcgtt ttccatcgaa accacccgtc agactttggg
     1081 gctggacacc ctcatccttt tgaacgactt taccgcgcag gcattggcgg taacgcagac
     1141 ttcaagcaaa gacctgatgc aggtaggcgg gcaaaagcct gtcgaatttg cccccaaagc
     1201 cgttatcggc cccggtaccg gcctgggcgt gagcggattg gtgcacagcc acgcaggctg
     1261 ggtggctttg gcgggcgagg gcgggcatac cagtttcccg ccgtttgacg atatggaagt
     1321 gctgatttgg cagtacgcca aaaacaaata cggccatgtt tccgccgaac gctttttgag
     1381 tggcgcgggc ttgagcttgg tttacgaggc tttggctgca aaacagaaag ccaaacccgc
     1441 caaactgatg ccgtctgaaa tcacggaaaa ggctttgagc ggcgcgtcgc ctttgtgccg
     1501 tcagactttg gacatcttct gcgccatgct cggcacggtt gcttccaacc tcgccctgac
     1561 gctgggcgcg cgcggcggcg tgtacctgtg cggcggcatt attccccgcg tgttggaata
     1621 tttcaaaact tccccgttcc gcagccgttt cgagaacaag ggcaggtttg aagcatatct
     1681 tgccgcgatt cccgtgtatg tcgtcttgag cgagttcccc ggaatttccg gtgcggctgc
     1741 ggctctcgac aaccatttga gaaacgttta accacagcgg ctccttgcag cggggctgca
     1801 ttatcgaagg gcatatcatt atgttaagca aaatcagcga atcactggca aacctttccg
     1861 gtgcggaacg caaagtcgcc gaatgtgcat tggcggaacc caaatggttc gttcatgccg
     1921 ccgttgccga aattgccgaa cgtgcgtccg tcagccagcc gaccgtcatc cgattctgcc
     1981 gcagcttggg ttataaaggg ctgcccgagt tcaagctcgc cttgtccgcc agcatcggtc
     2041 atgagggtat gccctatgtc cacgaagaac tcaatgccga cgacgatatg gcaagcgtgg
     2101 tcgagaaagt gttgggcaat gccgccgcct cgctgttggg cgaacgccgc ttcctgaaag
     2161 agtcggagct ggaaaacgcc attgccacgc tgatgcacgc ccgtcgcgtc gagttttacg
     2221 gtgtcggcaa ttccggtatc gttgcgcagg acgcgcaaca caagtttttc cgtttcggaa
     2281 tgtccacggt cgcctatgtc gatacgcaca cgcagctgat ggcagcatcc gttttgagcg
     2341 atcaggatgt tttggttgcc atttccaaca cgggttcgtc tatcgaactt ttggatgcgg
     2401 tcagcatcgc caaagaaaac ggcgcgtccg tcatcgcact gacccgcaac gattcgcctc
     2461 tggcgcaact tgccgactgc gtgttgagcg ttgccacaca ggaaaatgcc gaactctaca
     2521 cgcccatggt ttcccgcctc ttgcagcttg ccgtcatcga cattctcgcc atcggacttg
     2581 ccctgcgctt gggcgatgct gccagcctgc aattgcagaa aagcaaaaaa agcatacaca
     2641 acaagcacat cgattacgac aaagattgac cttcagacgg catcccacaa atgccgtctg
     2701 aaatgccgaa caacggccgt cggcggcttg cggcagtttc cggcagcctt ttcatcccac
     2761 aacaaaaaac ctcattcagg agcatataga tgaaacactt tcacgactta cccgcatggc
     2821 cgaaattgtg gaaccacttt gacgacagca aaacattgca tatgcgcgaa atgttcgagc
     2881 aagacccgca gcgtgcggaa cgctactggc tgcaagtcgg cggactgacg ctggactact
     2941 ccaaaaaccg catcaacgac gaaaccatgt cgcttttgtt cgagttggcg cgtgaagcag
     3001 gcgtgccgga gcggatgcgg cagatgttcc acggcgaaaa aatcaatacg acggaaaacc
     3061 gcgccgtcct ccacgtcgcc cttcgcaact gcaccaattc gccgattgtg gttgacggtg
     3121 aagatgtgat gccaaaagtc aaccgcgttt tgcaacgtat gggcgaattt gcacacgaag
     3181 tccgcagtgg aagctggctg ggctatacca accaagtcat taccgacgtt gtcaacatcg
     3241 gcatcggcgg atcggatttg ggcccgttga tgatgtgtac cgcgctcaaa cctttcggtc
     3301 atccacgcct caatatgcac ttcgtctcca acgtggacgg ctcgcaactg cgcgacgtat
     3361 tgtccaaagt ccaccccgaa accacgctgt tcattatcgc ctccaaaacc tttaccacac
     3421 aggaaacgct gaccaacgcg ctgaccgcgc gcgaatggtt tttgaatcat gcgggcgatg
     3481 aaagcgccgt cgccaaacat ttcgttgctg tttccaccaa ccaaaaagcc gttgccgaat
     3541 tcggcatcga caccgccaat atgtttgaat tttgggactg ggtcggcggc cggtacagcc
     3601 tgtggtccgc catcggattg ccgattatgc tgtatctcgg cgaagaaaac ttcatcgaaa
     3661 tgctcaacgg cgcgcacctg atggaccaac atttcatcaa cactccgctc gagcgcaacc
     3721 tgcccgccat cctcgccctt atcggcatct ggtacatcaa ctactacggc ggcggaagcc
     3781 acgtcatcgc accttacgac caacacctcc accgcctgcc caaattcatc cagcagctcg
     3841 atatggaaag caacggcaaa caggttacgt tggacggcaa agcagtcgga cacgaaacct
     3901 cgccgattat ttggggcgaa acgggcatca acggccagca cgccttcttc caactgctgc
     3961 accaaggcac gcacatcacc cccatcgacc tcatcgcctc gcttgaaaaa cgcagcaacc
     4021 tgcccggaca ccacgaaatc ctgcttgcca acgtcttcgc ccaagcagaa gcctttatgc
     4081 gcggcaaaac ccccgacgaa gtccgcgccg aactcaaagc gcagggtatg gatgaggcgc
     4141 gtatcgaaga gctggttccg cacaaaacct tctccggcaa ccgcccgacc aacctcatcc
     4201 tgatggataa ggtcaacccc cgcaatatgg gcagcctgat tgccatgtac gaacacaaaa
     4261 ccttcgtaca aggcatcatt tggggcatca acagcttcga ccagtggggc gtggaactcg
     4321 gcaaacaact ggctaaaacc attttgggcg aactgaccgg cgaaaccggg ccgcaaaagc
     4381 acgacagttc gaccgaacgc ctgatcaggc tttacctgca aaccaaccgc aaataaaacc
     4441 tgcggaaaaa tgccgtctga acgccgaccg tttcaaacgg catttttatc gaacaggaaa
     4501 accgtcggta aacttgcaga gcgtgtgcaa tcccgatatg atggtttgca taaatttaaa
     4561 catgtatgtt ccgcagctat ggcactgatt aaagagccgt tggacaaagc gaaacaaagg
     4621 aacgaagaac ttgaagcggc agaagaagcg gcggcgcagg aggcattggg tcgggagcag
     4681 gaagtcgacc gcgtatccga atgggaagaa cgctacaagc tgtcgcgcag cgagttcgag
     4741 caattctgga aaggattgcc tcaaaccgta cagaataagc tgcaagcctc acagaaaaca
     4801 tggaaaagcg ggatggataa aatctgtgcc aacaatgcga aagctgaagg tgaaacgcca
     4861 aacggcataa aattcagcga actggcatgc aaaacggcgg aaaccgaagc acgcttggaa
     4921 gagctgcaca accgtaaaaa agcccttatc gacgaaatgg ccagggaagc ggacaagaaa
     4981 gaactgccaa agcggctctg aacagcgcgg ttcaggcact gcccgccgat attgccgaaa
     5041 ccgtcatgcc cgagtaccgc aactgacaaa acggtttgaa tgccaaatgc gccgacggcg
     5101 acgaatacgg cgtggcacaa tctgactgcc gtaccagaga aatcaatgcg aaaaccaaag
     5161 aaatccaagg ttatctgatt gactgaaact tggatgcggg aatgtcggcg gcttttgcgt
     5221 ttttgtcgtt tttatagtga attaaattta aaccg
//
LOCUS       DAWEWP010000134         5198 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWEWP010000134 DAWEWP010000000
VERSION     DAWEWP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.134"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..480
                     /locus_tag="WGN53_001663"
     CDS             <1..480
                     /locus_tag="WGN53_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140632.1"
                     /translation="AAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRK
                     GAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVL
                     PDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            499..873
                     /locus_tag="WGN53_001664"
     CDS             499..873
                     /locus_tag="WGN53_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0140633.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            1258..2688
                     /locus_tag="WGN53_001665"
                     /pseudo
     CDS             1258..2688
                     /locus_tag="WGN53_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
     gene            2707..2970
                     /locus_tag="WGN53_001666"
     CDS             2707..2970
                     /locus_tag="WGN53_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140634.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            3077..4444
                     /locus_tag="WGN53_001667"
                     /pseudo
     CDS             3077..4444
                     /locus_tag="WGN53_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
BASE COUNT         1607 a         1100 c         1261 g         1230 t
ORIGIN      
        1 gctgctccta aagaaacaat aaacaatttg gcaaatttag ccaaagcaga acagcagatt
       61 ttattccgta ttgcccaacg cgatacgcaa ctggatgcat ggaagacggg atttaacaat
      121 agagtaagga aaggagcagg cttgcttgat gcaagtaata ttccgataac cattaacgga
      181 aaaaccatca aacctgtaca agccataagc ttaaagggag cacccgttta cagcggcgta
      241 agcgaacagg agatttttgc gctttatcgg cagatgactg gccagaatcc gaattttaga
      301 gttttgcctg acggaagatt agcaaatggc attatcagta ctggagaatg ggcaggaaca
      361 aaaattgcat taagaaattt ttcaaaaaca gagaattcaa ctcaagcacg atggacatta
      421 gatttgcaga atcctccatc atttattaaa ggtactaaat tggagcttaa attccaataa
      481 tttacaaagg attttaccgt ggatgagaaa caaaaaatta agattcttga ttttcaaatc
      541 gatttatcct caatttttaa ctcttataaa aatcaaatgg gtattaatat tcaagataaa
      601 aacttaaaaa acaatttctg ttcttttatg gaagaactgt taaatgacgg ttcaatccgt
      661 ttacatgatt ataccgacgg tatcggaatt cctctaactg gaacttcaaa agaacaagtg
      721 cagaaattga aagacatatg gcctactttg gaagatgccc aagcaatatg gcctgaagac
      781 ccttggtatt acttagaatg gctttggtgg gatattgcgt gtccaataga tttggccgat
      841 ttgccgaata ttgatattta tgagcaagcg taggtatggt tagccgcctt tagcggcgta
      901 accgtacgca tatcagcaaa ctttataaaa taacaaggcc gtctgaaatc tgtttttcaa
      961 ctttttcaga cggccttgca acttggcatt tcattcgtac ggttacgccg ctaaaggcgg
     1021 ctaaccgtac ctacgagctc tgataaaaat gatttatgga agcaagctgt agcctgcatg
     1081 aaacctaaaa tccatgcgta aggtgtgtgc ttcagcgcgc acgcgttcca tgatttacgg
     1141 ctcaatgccg tctgaaaagc tcaatttttt cagacggcat ttgttatgca agtaaatatt
     1201 cagattccct atatactgcc cagatgcgtg cgtgctgaag acacccccta cgcttgctat
     1261 ttgaaacagc tccaagtcac caaagacgtc aactggaacc aggtacaact ggcgtacgac
     1321 aaatgggact ataaacagga aggcttaacc ggagccggag cagcgattat tgcgctggct
     1381 gttaccgtgg ttactgcggg cgcgggagcc ggagccgcac tgggcttaaa cggcgcggcc
     1441 gcagcggcaa ccgatgccgc attcgcctcg ctggccagcc aggcttccgt atcgctcatc
     1501 aacaataaag gcgatgtcgg taaaaccctg aaggagctgg gcagaagccg cacagtgaaa
     1561 aaactggttg tagcagcggc catcgcaggc gtatccaaca aaatcggtgc ttcctccctt
     1621 gccacttgga gcgaaacccc ttgggtaaac aacctcaacg tcaacttggc taatgcgggc
     1681 agtgccgcgc tgattaacac cgccgttaac ggcggcagtc tgaaagacaa tctggaagcg
     1741 aatatccttg cggctttggt gaatactgcg catggagagg cagcaagtaa aatcaaacaa
     1801 ttggatcagc actacattgc ccataagatt gcccatgcca tagcgggctg tgcggcagcg
     1861 gcggcgaata agggcaagtg tcaggacggc gcgattggtg cggtcgtggg ggagattgtc
     1921 ggggaggctt tggttaagaa taccgatttc agcggtatga ctgcttctga aattgaaaaa
     1981 gctaaagcga atattactgc gtatgcaaaa ttggtagccg gagcgactgt aggtgttaca
     2041 ggaggcaatg ttgatgtggc ggcaaatgct tccgaaacag ctgttaaaaa taatgcatta
     2101 gatattattt gggatatcgg caacctcgta tgggacggcg gtaaatggat ttacgccaaa
     2161 tctattggcg ataagcagat ggctcgagaa gcggcgattg attttggtgt ggatgccgcc
     2221 gcagctgccg ttccctttgt tccggcaggt gcgactaaaa tcagccgagg cggggcttat
     2281 gttctgaagg cgggagacga agcagttgat acggctaaag ccatacagga aattcagaag
     2341 cagaccggaa tcaagcttac ttatgataag gttaataagg tttggacaac accggcgggg
     2401 ttagattatg ggttagatgc taagcatggt aataggatta aacatgtttt agcccataca
     2461 attccaaatc caaacaaacc tgttcattct gtttttaatg tgtcccgtaa agaagttttg
     2521 cctttggttg atgaagcttg gagaatgaaa ggaaatcctt tgccaaatga ttcatccgta
     2581 tatcttgtag atatgaagaa acctattgga acaaaaggag aaacaaaagt gcggattgtt
     2641 gtgcaaaaag gaacaaataa aatcatttct gcatatcctc agaaataatt aagaaaggaa
     2701 tctcttatgg ataaagaaat taaaatttgc ccaagatgtg agcaaggcta cctttatcat
     2761 gcaaagccta aatatttctc tggggaggtc attttatgcg atgaatgtta tgctatgtgg
     2821 cttggggata tgaaaatttt ttacggacaa tatggaaaag atttttatga ttatcatgag
     2881 tttatgaaag ataaaggcat agaagaaata aatatgtggg aaggagagct ttttgatcac
     2941 ccatattatg aggatgaaaa atttaaataa ttgattttct gttccccgaa tttgggaaat
     3001 acgatgatat tttaaaccca aatattattt aaagtagcaa taggccgtct gaatatccgt
     3061 ttttcagacg gcctcaatgc aactgctggc agccgaaggc attcaccaac accaattgaa
     3121 tgttcagaaa agtacccgtt tcatcggcat caaagtgggt aaaagcaatt acagcaaaaa
     3181 cgagctgaac gaaaccaaac tgcccgtacg cgttatcgcc caaacagcca aaacccgttc
     3241 cggctgggat accgtactcg aaggcaccga attcaaaacc accctttccg gagccgacat
     3301 acaggcaggg gtgggtgaaa aagcccgagc cgatgcgaaa attatcctaa aaggcatcgt
     3361 taaccgcatc caaaccgaag aaaagctgga atccaactcg accgtatggc aaaagcaggc
     3421 cggaagcggc agcacggttg aaacgctgaa gctaccgagc tttgaagggc cggcactgcc
     3481 taagctgacc gctcccggcg gctatatcgc cgacatcccc aaaggcaacc tcaaaaccga
     3541 aatcgaaaag ctggccaaac agcccgagta tgcctatctg aaacagctcc aagtagcgaa
     3601 aaacatcaac tggaatcagg tgcagcttgc ttacgacaga tgggactaca aacaggaggg
     3661 cttaaccgaa gcaggtgcgg cgattatcgc actggccgtt accgtggtca cctcaggcgc
     3721 aggaaccgga gccgtattgg gattaaacgg tgcggccgcc gccgcaaccg atgcagcatt
     3781 cgcctctttg gccagccagg cttccgtatc gttcatcaac aacaaaggcg atgtcggcaa
     3841 aaccctgaaa gagctgggca gaagcagcac ggtgaaaaat ctggtggttg ccgccgctac
     3901 cgcaggcgta gccgacaaaa tcggcgcttc ggcactgaac aatgtcagcg ataagcagtg
     3961 gatcaacaac ctgaccgtca acctagccaa tgcgggcagt gccgcactga ttaataccgc
     4021 catcaacggc ggcagcctca aagacaactt gggcgatgcc gcactgggtg cgatagtcag
     4081 taccgtacac ggagaagtag cgagcaaaat caaatttaat ctcagcgaag actacattac
     4141 ccacaagatt gcccatgcca tagcgggctg tgcggcagcg gcggcgaata agggtaagtg
     4201 tcaggatggt gcgatcggtg cggctgtggg cgagatagtc ggggaggctt tgacaaacgg
     4261 caaaaatcct gccactttga cagctaaaga acgcgaacag attttggcat acagcaaact
     4321 ggttgccggt acggtaagcg gtgtggtcgg cggcgatgtg aatacagcgg cgaatgcggc
     4381 taaagtcgcg attgaaaata acctattatc tcaagaagag tatgctctta gagaaaaatt
     4441 gatcaaaaaa gccaaaggga aaggcctatt atctttagat tggggcagcc tgaccgaaca
     4501 agaggcaagg cagtttatct atttgattga gaaagatcga tattctaatc aattgcttga
     4561 ccgatatcaa aaaaatccaa gtagtttaaa taatcaagaa aaaaatattc ttgcatattt
     4621 tattaaccaa acctctggag gtaacacagc ttgggcagct tcgatactga aaacgcccca
     4681 gtcaatgggt aatctcacta ttccttccaa agatattaat aacaccttat cgaaagccta
     4741 tcaaacattg agtcgttatg attcttttga ttacaaatca gctgttgccg cacaacctgc
     4801 actttactta ttaaacggac cgcttggctt cagtgtcaaa gcagctactg tggcagcagg
     4861 aggatataac attggacagg gagcgaaagc aatctctaat ggagaatatc tgcatggtac
     4921 agttcaggtt gttaatggca cattgatggt tgcaggatct gtatctgcac aggctgcaat
     4981 atcggccaag cctgcacctg ttacccgtta tctgagcaat gacagtgctc ctgctttaag
     5041 acaagcttta actgctgaaa gccagagaat ccgcatgaaa ctgccggaag agtatcgaca
     5101 aatagggaat cttgcgatag caaaaattga tgttaaagga ttaccgcaaa ggatggaagc
     5161 atttagttct ttccaaaaag gggaacatgg atttattt
//
LOCUS       DAWEWP010000135         5165 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWEWP010000135 DAWEWP010000000
VERSION     DAWEWP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.135"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            397..5103
                     /gene="iga"
                     /locus_tag="WGN53_001668"
     CDS             397..5103
                     /gene="iga"
                     /locus_tag="WGN53_001668"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ0140635.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGNGEHHWNITFG
                     TNSKIGSTAVRLAGNEKDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGIN
                     NDITWLGAGIDVTDGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVI
                     LNQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFE
                     HIRNVDEGARIVNHNTSHASTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRR
                     PIPQGKDLYYKNYRYYALKSGGSVNAPMPENGVTENNDWVFMGYTQEEAKKNAMNHKN
                     NQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLS
                     GRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQSASFSSGRNV
                     SNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGN
                     VNLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNN
                     ASDAQSANQYHTLKINHLSGNGHFHYLTHLAENLGDKVLVKESASGHYQLHVQDKTGE
                     PNQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRV
                     KPVPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAE
                     AERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQKVEA
                     EREAQALAVRRKAEAEEAKRQAAELARQQEEARKAAELAAKQKAETERKAAEIAEQKA
                     EAEREAAELAKQKAEEEGRQAAQSQPKRRNRRAIPPELSSDATTRALPRIARNSNPDA
                     SDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQAAAQPQ
                     AQAAAQPQAQAVAQADAVSTNTNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEK
                     NSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQ
                     ASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQKANFNRTSIQTGLTLGN
                     TLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLT
                     VKPLLSAAYFANYGKGGVNVGGNSFAYKADNQQKYSAGAALLYRNVTLNVNGSITKGK
                     QLEKQKSGQIKIQIRF"
BASE COUNT         1742 a         1209 c         1121 g         1093 t
ORIGIN      
        1 acggaaacct gcaccgcgtc attcccacga acccacatcc cgtcattacc acgaaagcgg
       61 gaatctagga cgcagggtta agaaaaccta catcccgtca ttccctcaaa aacagaaaac
      121 caaaatcaga aacctaaaat cccgtcattc ccgcaaaagc gggaatccaa aaacaaaaag
      181 ccgcagaaat ttaacggaag aaccgaaccc cgaaaatcgg cttcccgcct tcgcttgaat
      241 aacagaacaa aggaagcgga aataaaaaca tcgggattct ttttttaaat tggcaataaa
      301 acaccaaaat taatgattca cacaacaaaa aatagtcaac accgccaaac cggcgtaaaa
      361 tacaaaacat tatcaccctt aaaacggtaa aaccttatga aaaccaaacg ttttaaaatt
      421 aacgccatat ccttatccat ctttcttgcc tatgccctta cgccatactc agaagcggca
      481 ttggtcagag acgatgtcga ttatcaaata ttccgtgact ttgcagaaaa caaaggcaaa
      541 ttttttgtcg gcgcaaccga tttatcagtg aaaaacaaac aaggtcaaaa catcggcaac
      601 gcattatcca acgtaccgat gattgatttt agcgttgcag atgtcaacag acgtacatta
      661 accgtcatcg atccccagta tgccgtcagc gtcaaacatg taaaaggaga cgaaatctct
      721 tattacgggc atcacaatgg gcacttagat gttagcaatg atgaaaacga atatcgttca
      781 gttgcacaaa atgactacga accaaataaa aattggcatc acggtaatca aggtcgtctt
      841 gaagactaca acatggcacg ccttaataaa ttcgtaacag aagtcgcacc tattgcacca
      901 accagtgcag gcggcggcgt tgaaacctat aaagataaaa accgtttttc tgagtttgtg
      961 cgagttggag caggtacgca atttgaatat aacagccgct acaatatgac agagctttca
     1021 cgagcttatc gttatgccat tgcaggtacg ccttatcaag acgtgaatgt cacatcgaat
     1081 ctaaatcaag aaggcttgat tggcttcggt gataattcaa aacatcattc cccagaaaaa
     1141 ctcaaagaag ttttatctca aaacgcattg actaactatg ctgtgttagg cgatagcggt
     1201 tcaccattat ttgcttatga taaacaagaa aaacgctggg tctttttagg tgcttacgat
     1261 tattgggcag gctatcaaaa aaactcttgg caagaatgga atatctataa aaaagaattt
     1321 gcagataaaa tcaaacaacg cgataacgcc ggcaccatca aaggtaatgg agaacatcat
     1381 tggaacatta cttttggcac aaacagcaag attggttcta cagcggtaag gcttgccggc
     1441 aatgaaaagg atgcaaacaa cggacagaat gttacctttg aagacaacgg cactttggta
     1501 ttggatcaaa acatcaacca aggcgcgggc ggtctgtttt tcaaaggcga ttacacagtc
     1561 aaaggtataa ataatgacat cacttggcta ggtgcgggga ttgatgttac cgacggcaaa
     1621 aaagtcgttt ggcaagtcaa aaatccgaat ggcgacagat tggcaaaaat cggcaaaggc
     1681 acattagaaa taaacggcac aggcgttaac caagggcaat taaaagtcgg cgacggtacg
     1741 gttattctga atcaacaagc cgatgccgac aaaaaagtcc aggctttctc ccaagtcggc
     1801 attgtcagcg gacgcggtac attggtatta aatagttcaa atcagattaa tcccgataac
     1861 ctatatttcg gtttccgtgg cggtcgtttg gatgccaatg gcaatgactt gacttttgaa
     1921 cacatccgca acgtggatga aggcgcgcgc attgtcaacc acaacacaag ccacgcctcc
     1981 acaatcacgc taacgggtaa atctttaatt accaatccga acagcctttc ggtacatagc
     2041 atacaaaatg actatgacga agatgattat agttattact accgaccaag gcgcccgatt
     2101 ccgcaaggca aagaccttta ttacaaaaac tatcgttatt acgccctaaa atccggcggc
     2161 agcgtgaacg caccgatgcc cgagaacggt gtaacagaaa ataacgactg ggtctttatg
     2221 ggatatacgc aagaagaagc caagaaaaac gcgatgaacc acaaaaacaa tcagcgtatt
     2281 agcggtttta gcggtttctt tggcgaagaa aacggaaaag gacataacgg tgcattaaac
     2341 cttaatttca acggcaaaag cgcgcaaaac cgtttcttgt taacaggcgg cactaattta
     2401 aacggaaaaa taagcgtaac tcaaggcaat gtcttgttat caggtcgtcc aacaccacac
     2461 gcaagagatt ttgtgaacaa atcttcagcc cgaaaagacg cacatttctc caaaaacaat
     2521 gaagtcgtat ttgaagacga ctggataaac cgcacattca aagccgcaga aattgcggtt
     2581 aatcaatccg catcattctc ttccggtaga aatgtatcca acattaccgc caacatcacc
     2641 gcgacagaca acgccaaagt aaatttgggt tacaaaaacg gcgatgaggt ttgcgtgcgc
     2701 tcggactata ccggttatgt tacctgcaac acaggcaact tatccgataa ggctttaaac
     2761 agctttggag cgacacagat taacggcaat gtgaatctga accagaatgc ggcattggtc
     2821 ttgggcaagg ctgcattatg ggggcaaatt caagggcaag gaaacagccg tgtcagccta
     2881 aaccaacata gcaaatggca tttgactggc gacagccaag tacacaatct gtcattggcg
     2941 gatagccata ttcatttgaa caatgcctcc gatgcgcaaa gtgcaaatca ataccacacg
     3001 ctcaaaatca atcatttatc cggtaacggg cattttcatt atctgacgca cttggcggaa
     3061 aatcttgggg ataaagtgct tgtgaaggaa tccgcatccg gtcattatca gctccatgtt
     3121 caagataaaa caggcgaacc caatcaggaa gggctgaatc tctttgatgc atcatccgta
     3181 caagaccgct cccgcctttc tgtttccttg gcgaataacc atgtagattt aggcgcattg
     3241 cgttatacaa tcaaaacaga aaacggtatt acccgattgt acaatcctta tgccgagaac
     3301 cgccgccgag tcaaaccggt cccgtctcct gccacaaaca cggcttctca agcacaaacg
     3361 gacagtgcac aaattgccaa gcctcaaaat atcgtcgtcg caccgcctag cccgcaggca
     3421 aatcaagccg aagaagcaaa acgccaacaa gcaaaagcgg agcaagtgaa gcgtcagcaa
     3481 gcagaagcag agcgtaaatc ggcagagtta gcaaaacaaa aagcagaagc cgaacgcgaa
     3541 gcaagagagc tggcgacccg ccaaaaagca gaacaagaac gcagcagcgc cgaacttgca
     3601 cgtcgacatg aaaaagaacg cgaagcggca gagttgtcgg caaaacaaaa agtcgaagcc
     3661 gaacgtgagg ctcaagcatt ggcggtacgc cgaaaagcag aagccgaaga ggcaaaacgc
     3721 caagctgcag agcttgcacg ccaacaagaa gaagcacgaa aagctgcaga gttggctgct
     3781 aagcaaaaag cggaaactga acgcaaagca gcagaaattg ccgagcaaaa agcggaagcc
     3841 gaacgtgaag ccgccgaact tgccaagcaa aaggcggaag aagaaggtcg ccaagcagca
     3901 caatctcaac ccaaacgtcg taaccgcagg gctataccac cggaactgtc atctgatgca
     3961 acaacccgag ctttgcctag gatagcgaga aattcaaatc ctgatgccag cgattatgaa
     4021 gaaatcccct tggatgcgtt ggaagatgaa gatgtatccg aatcggttga cacatcagac
     4081 aaacagcctc aagacaatac ggaacttcat gaaaaagttg agacagtgag tttgcaacca
     4141 agagccgcgc agccgcgagc tcaagccgcc gcgcagccgc aagcccaagc cgccgcgcag
     4201 ccgcaagccc aagccgtcgc gcaagccgat gcagtcagca ccaataccaa ctcggcttta
     4261 tctgacgcaa tggcaagcac gcaatctatc ttgttggata caggtgcttc attaacacgg
     4321 cacattgcac aaaaatcacg tgctgatgcc gaaaaaaaca gcgtttggat gtcgaacatc
     4381 ggttatggcc gtgattatgc ttccgcacaa tatcgccggt ttagttcgaa acgcacgcaa
     4441 acacaaatcg gcattgaccg cagcttgtcc gaaaatatgc agataggcgg cgtattgact
     4501 tactctgaca gtcagcatac ttttgatcag gcgagcggca aaaatacttt tgtgcaagcc
     4561 aacctttatg gtaagtatta tttaaatgat gcttggtatg tggccggcga tattggtgca
     4621 ggcagcttga gaagccggtt acaaacacag caaaaagcaa actttaaccg aacaagtatc
     4681 caaaccggcc ttactttggg caatacgctg aaaatcaatc aattcgagat tgtccccagt
     4741 gcgggaatcc gttacagccg cctgtcatct gcagattaca agttgggtaa cgacagtgtt
     4801 aaagtaagtt ctatgtcagt gaaaacactg acggccggac tggattttgc ttatcggttt
     4861 aaagtcggca accttaccgt aaaacccttg ttatctgcag cttactttgc caattatggc
     4921 aaaggcggcg tgaatgtggg cggtaattcc ttcgcctata aagcagataa tcaacagaaa
     4981 tattcagcag gtgccgcgtt actgtaccgt aatgttacat taaacgtaaa tggcagtatt
     5041 acaaaaggaa aacaattgga aaaacaaaaa tccggacaaa ttaaaataca gattcgtttc
     5101 taaaatacta aattcatagc aaaataaaat gccgtctgaa ctcaggcttc agacggcatt
     5161 tttat
//
LOCUS       DAWEWP010000136         5075 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWEWP010000136 DAWEWP010000000
VERSION     DAWEWP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.136"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(162..3260)
                     /locus_tag="WGN53_001669"
     CDS             complement(162..3260)
                     /locus_tag="WGN53_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0140636.1"
                     /translation="MNKTLKRQVFRHTALYAAILMFSHTGGGGAQAETRNYAIVMNEQ
                     NQPNVKQEGSYSTLREKDRKRKFDFNANRGGGGSVFFDNTDTLVSQQRGTAVFGTATY
                     LPPYGKVSGFDEKRLTERGNALNWINTTHPGLIGYSYAGVVCRDSTSCPKLVYKTRFS
                     FDNTGLAKNAGSLDRHPDPSRENSPIYKLKDHPWLGVSFNLGSENTVKNGNSFNKLIS
                     SFSEDNNNQTIVSTTEGSPISLGDQQREHTAVVYYLNAKLHLLDKKQIQNITDKTVQL
                     GTLRPRVETTGRSWLNFWATWKIEDKGNITVRLDLPEVKAGRCVNANNPNKSTKAPSP
                     ALTAPALWFGPVQNGKAEMYSASVSTYPDSSSSRIFLQNLKRKNDSNRPGRHSLETLT
                     ENDIKSRQPNFTGRQTIIRLDSGVQQIKLQGNEVANFNRNDGKNDTFGIVSEYGLTPD
                     ANEWKKVLLPWTVRAFNDDGRFNTVNKEENNGKPKYSQRYRIRENGNNGNNGNNGKRD
                     LGDIVNSPIVAVGEYLATSANDGMVHIFKQSGGDKRSYNLKLSYIPGTMPRKDIQNTE
                     STLAKELRAFAEKSYVGDRYGVDGGFVLRQVELSGKKHVFMFGAMGFGGRGAYALDLT
                     KAENGNPTAVSLFDVKHDNNGKNSNNSVQLGYTVGTPQIGKTHNGKYAAFLASGYATK
                     TIDSTDNKTALYVYDLESSGTLIKKIDVPGGKGGLSSPTLVDKDLDGIVDIAYAGDRG
                     GNMYRFDLSGNNPNSWTVRPIFEGTKPITSAPAISQLKDKRVVIFGTGSDLSEDDVDN
                     KDIQHVYGIFDNDTDTGTAQDGQGKGLLEQVLSEENKTLFLTDYKRSNGSGSKGWMVK
                     LQPRQRVTVKPTVVLRTAFVTIRKYEDNGCGAETAILGINTADGGKLTKKSARPIVPA
                     ANQAVAQYSGHKQTTKGKSIPIGCMQKGNEIVCPNGYVYDKPVNVRYLDEKKTDGFST
                     TADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMNDLDSLDITGPTCGMKRISWREV
                     FY"
     gene            complement(3789..4868)
                     /gene="apbC"
                     /locus_tag="WGN53_001670"
     CDS             complement(3789..4868)
                     /gene="apbC"
                     /locus_tag="WGN53_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ0140637.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSIDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSNGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
BASE COUNT          990 a         1279 c         1434 g         1372 t
ORIGIN      
        1 atcggtttga cggtatttaa ttgaattgtg gaaattgatg gattcagtga gattggcgag
       61 atgaagccta ccctatagcc cgccttttac gaacccgccc ctcccgaaaa acgcaaaaaa
      121 tgccgtccga aaacctttcg gacggcattt tcgcgtgcaa atcagtagaa gacttcacgc
      181 cagctgattc gtttcatacc gcacgtcggg ccggtaatgt ccaagctgtc caaatcgttc
      241 atcagcaggg tgcgcacccc tttttgggag aagcagcggt tgtttttgcc ggaacgcttg
      301 ccggcggggt ctataccgct gccgcccgca tcgccgtctg ccgttgttga aaatccgtct
      361 gttttctttt cgtccaggta gcgcacatta accggtttgt cgtaaacata tccgttcggg
      421 cagacgattt cattgccttt ttgcatacaa cctataggga tggatttgcc tttggtggtt
      481 tgcttatggc cggaatattg cgcgacagcc tgattggctg ccggcacaat cgggcgcgcg
      541 cttttcttgg tcagcttgcc gccgtcggcg gtattgatgc ccaaaatggc ggtttccgcg
      601 ccgcagccgt tgtcttcata tttgcggatg gttacaaagg cggtacgcaa taccacggtc
      661 ggtttgacgg taacgcgctg tctgggctgc aatttcacca tccacccctt actgcccgat
      721 ccgttggatc gcttgtaatc ggtcaggaat aaggttttat tttcctcact aagcacttgc
      781 tcgagcagcc ctttgccctg cccgtcttgc gccgtacccg tgtctgtgtc attgtcaaaa
      841 ataccgtaaa cgtgttggat gtctttgttg tccacatcat cctcactcaa atcactgccc
      901 gtgccgaaga tgaccacgcg tttgtctttc agttgggaaa tggcgggcgc ggaagtaatc
      961 ggttttgtgc cttcaaaaat agggcgtaca gtccaactgt tcgggttgtt gccgcttaaa
     1021 tcaaagcggt acatattccc gccgcgatcg ccggcatagg cgatatcaac aatgccgtcc
     1081 aaatctttat ccaccagcgt gggggacgaa agcccgccct tgccgccggg tacatcgatt
     1141 tttttaatca gcgtaccgct gctttccaaa tcatacacat acagcgcggt tttattgtct
     1201 gtgctgtcaa tggttttagt cgcataaccg gaggcgagga aggcggcgta tttgccgttg
     1261 tgggttttgc cgatttgcgg cgtgccgacg gtgtagccta attgcacgct attattgcta
     1321 ttcttgccat tattgtcatg tttgacatca aacagggaaa cggcggtcgg gttaccgttt
     1381 tcggctttgg tcaaatccaa ggcgtatgcg cctctgccgc caaaacccat cgcgccgaac
     1441 ataaacacgt gttttttccc gctcagttcg acttggcgca aaacaaagcc gccgtccacg
     1501 ccgtagcggt cgcccacata gcttttttcg gcaaaggcgc gcagctcttt ggcaagggtg
     1561 gattcggtgt tttgaatatc cttgcgcggc atcgtacccg ggatataact gagcttcaga
     1621 ttgtagctgc gcttgtcccc gccgctttgt ttgaagatat gcaccatccc gtcgttggcg
     1681 gaagtagcca aatactcgcc gaccgccacg atggggctgt tgacgatgtc gcccaaatca
     1741 cgcttgccgt tgttgccgtt gttgccgttg ttgccgtttt cgcggatgcg gtatctttgg
     1801 ctgtattttg gcttgccgtt gttttcttct ttgttgactg tgttaaatcg accgtcatca
     1861 ttgaaagcac gaaccgtcca aggcagcagc acttttttcc actcattggc atcaggcgtg
     1921 aggccatatt cactaacaat gccgaaagtg tcgtttttgc cgtcatttct attaaaattg
     1981 gcgacctcat tgccttgaag tttgatctgc tgtacgccgc tatccaatcg gatgattgtt
     2041 tgccgccctg tgaaattcgg ctgtcgactt ttaatatcgt tctcagtcaa ggtttcgagg
     2101 gaatggcggc cgggtctgtt ggagtcgttt tttcttttca gattttgaag gaagatgcgg
     2161 ctgctcgaac tgtcggggta ggtggaaacc gaagcggaat acatctccgc cttaccattt
     2221 tgcacagggc cgaaccacag cgcgggggcg gtcagtgcgg gggaaggggc tttggtactc
     2281 ttattggggt tatttgcgtt gacgcagcgg cctgctttga cttccggcag gtcgaggcgg
     2341 actgtaatgt tccctttatc ttcaatcttc cacgtagccc aaaaatttag ccagcttcgt
     2401 cctgtcgtct cgacgcgcgg tctcaaggtg cccaactgca ctgttttgtc ggtgatattt
     2461 tgaatctgtt ttttatccag caggtgcagc ttggcgttca gataatagac cacggcggta
     2521 tgttcgcgct gctggtcgcc aagggaaata ggggagcctt ctgtcgtaga gacgatggtt
     2581 tgattattat tgtcttcact aaaagaagat atcaatttgt tgaatgagtt gccatttttg
     2641 acggtattct cgctgcccaa attgaaagac acgcccaacc atggatgatc cttcaatttg
     2701 taaatgggcg aattttcgcg gcttgggtcc gggtgcctat ccaggctgcc cgcatttttt
     2761 gccaaaccgg tgttgtcgaa ggaaaatcgg gttttataga caagtttggg acagcttgtg
     2821 ctgtctctgc atacgacacc ggcgtagctg tagcctatca accctgggtg ggtcgtatta
     2881 atccaattaa gggcattgcc gcgctctgtc agcctctttt catcaaaacc ggaaaccttg
     2941 ccgtagggcg gcaggtaggt ggctgtgcca aaaacggcag tacctctttg ttgggaaaca
     3001 agggtatcgg tattgtcgaa aaagacagaa cctccacccc ctctgttcgc attaaagtca
     3061 aatttgcgtt tcctgtcttt ttcccttaag gttgaatatg aaccctcctg ctttacattc
     3121 ggctgatttt gttcgttcat gacaatagcg tagttacggg tttccgcctg cgcccccccc
     3181 ccgccggtat gggaaaacat caagatggcg gcataaagcg cggtatggcg gaaaacctgc
     3241 ctttttaaag ttttattcat cgtatttcct tttcggtcaa accccccgcc cttcgggcat
     3301 ccgtccttcg gggcggcagg atcaggcttt gtccgggagg ggtgcaagcc cttccgaatc
     3361 agggcggcgc cttatgtgcc gccctgcgtg ttggaacata ttttatccct taaatcttgc
     3421 ttcgcgggat accgtcggcg gcaggcgcgc cgcaaacggc gggattgggc aaaccgcccg
     3481 attccgcgcc cgccgaattg ccggttggaa aacccctttg gcccggctgc caaaggggga
     3541 tgttcggaaa agtaacacgc ccctttggcc ggatgcaatg tatagggtta ccgcattatt
     3601 ctacccttgc gcgctgatca tggtaaagcg gttttcggca atccgcaacc gcctccttgg
     3661 cccgccattg ccgcgaaagc gggaatccgg aacgtaaaat ctcaaaaaaa ccgttttatc
     3721 cggcaagttt gcccgccggc aggcgcgggg cttgggacgg cattgctgtt gcggtttcgg
     3781 acgcggcttt attcgacgac gattttgggg aagcggctgc tgaagtcttt gcctttgtcg
     3841 gcaatgctca gggcgatttg gaatgcggca tcggtgtaga ttttggcgat ggcggggtgt
     3901 tcgtcgaaca gttgcgccgg tgtgccgccg tccatggctt cgcgcacggg caggcttagg
     3961 ggaagctgtc cgagcagggg gacgttgagg cgggccgcca aatctttgcc gccgtccgtg
     4021 ccgaacagtg cttcgctatg cccgcagttg gagcagatgt ggacggacat gttttccaat
     4081 acgcctaaaa tgggaatgtt gaccttgcgg aacatatcca cagccttgcg cgcgtctatc
     4141 agcgcgatgt cctgcggcgt ggttacgatg acggacccgg taacggggat gcgttgggac
     4201 agtgtgagtt ggatgtcgcc cgtacccggg ggcaggtcga taaacaggta gtccacttcg
     4261 tcccactcgc tttgaaacat cagttgctgc aaggcttggc tgaccatcgg cccgcgccag
     4321 acgacggctt ggtcggtgtc gacgagaaag ccgatggaca taacctgtat gccgtttgaa
     4381 gattcgacag gaatcagttt ttggtttttc tggtcgggtt tgcggtcgtg tacgcccagc
     4441 atcgtgggct ggctagggcc gtaaaggtcg gcatcgagca cgccgacgcg cgcgcccatg
     4501 cgcgccattg cggcggcaag gttggcggtg gttgttgatt tgcccacgcc gcctttgcca
     4561 gaggcgacgg cgatgatgtt tttcacgcct ttgatggtgg taacgccggg ctggactttg
     4621 tgtgtgccga tttcggtgtc tatggacaga tggatgtgtg tgtcgcctgt cagcggcatc
     4681 aggatttcct gtatgcggtc ggctgtttct gaggcaatgt gcgcgacggg gaagccgaaa
     4741 tgcagggcga tatggatgcc gtctgaacgc tgttcgaccg aacggacggc cttttcgccg
     4801 ccgagcgtgc gtgccgtatt cggaacggcg acggtgtcga ggagggtgcg gatgttttgt
     4861 atgttcataa tgtgtgtttc tgtgtgcagt ttgggaaacg ggcggaaagt gtacattgat
     4921 ttcgggcggt ttgcctttcc ttgtgcgaac ttttacacgg tatgcggcgg attgtgaaaa
     4981 tccgttgtca aaatatcccg tgcaaacagt ggtctgcaat taagcctgta aaatttgctt
     5041 gacgaggtag gatggtgctg tataatgcac ggctt
//
LOCUS       DAWEWP010000137         5003 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWEWP010000137 DAWEWP010000000
VERSION     DAWEWP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.137"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            12..422
                     /locus_tag="WGN53_001671"
     CDS             12..422
                     /locus_tag="WGN53_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140638.1"
                     /translation="MVQTVFFYPKETIMSKDKNQITVGINREIIDTETDSSAEFHVIR
                     HVSLDYATGFNVVLLDSYVRKSTFERRGRSVGSMQLNIPGVPPRGTDVLDWAYQAVVA
                     AVEGGAVDAYGQPISANSLTGAELVYADLPEAAI"
     gene            486..911
                     /locus_tag="WGN53_001672"
     CDS             486..911
                     /locus_tag="WGN53_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140639.1"
                     /translation="MSEPIITPAAVYIFGGVVGAGYLLGMPVDAVVLGAVASAAVTMI
                     SEPKSRWMVVAYIVIGGLLGGAFAPPLTHFLIGEVVGSHPSLGEQRIQFAHAFAPVVV
                     GLLWQVLLRFARAVWPSFEKHADDIVEWLLNLLPWRPKK"
     gene            908..1183
                     /locus_tag="WGN53_001673"
     CDS             908..1183
                     /locus_tag="WGN53_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140640.1"
                     /translation="MKLLLILSAAPSLISAYLMICRLNGRKWSALSPEAWAYLAFLGG
                     AIYTFYIVFAYGLTPSLGKFMMDIAACVYFGSRSVRVVKWRHRHGLK"
     gene            1322..1795
                     /locus_tag="WGN53_001674"
     CDS             1322..1795
                     /locus_tag="WGN53_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0140641.1"
                     /translation="MQVTKNFSLRELTRSETARRAGVPNEPSPADMDNIYYTAQQLEK
                     IREYVGRPIIVTSCFRSERVNKLVGGSPTSAHRHGLAADCDASGMTSPAFAKLLIKMR
                     DEGALKFDQLILEFPERGDGAWVHIGFRRNSPQRNQILTATKKNGKTVYLPGLHP"
     gene            1807..2316
                     /locus_tag="WGN53_001675"
     CDS             1807..2316
                     /locus_tag="WGN53_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140642.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRNRNLPATTTAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAVSSQRAE"
     gene            complement(2369..2716)
                     /locus_tag="WGN53_001676"
     CDS             complement(2369..2716)
                     /locus_tag="WGN53_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0140643.1"
                     /translation="MYIPDKGDIFHLNFDPSSGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSSGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(2718..2954)
                     /locus_tag="WGN53_001677"
     CDS             complement(2718..2954)
                     /locus_tag="WGN53_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEZ0140644.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDVGNEVV"
     gene            complement(3313..3804)
                     /locus_tag="WGN53_001678"
     CDS             complement(3313..3804)
                     /locus_tag="WGN53_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ0140645.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACAIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(4242..4610)
                     /locus_tag="WGN53_001679"
     CDS             complement(4242..4610)
                     /locus_tag="WGN53_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEZ0140646.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMACLDGALSRIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
BASE COUNT         1230 a         1210 c         1235 g         1328 t
ORIGIN      
        1 aaaccgtctg aatggttcag acggtctttt tttatccaaa ggaaaccatc atgtcaaaag
       61 acaaaaatca aatcaccgtc ggaatcaacc gtgaaatcat cgataccgaa accgacagtt
      121 ctgccgagtt tcacgtcatc cgacatgtgt cgctggatta tgcaaccggc tttaatgtgg
      181 tgttgttaga cagctatgtg cgcaaatcga catttgaacg tcgcggacgt tcggtcggtt
      241 cgatgcagtt gaatattccg ggcgtgccgc ctcgcggtac ggatgtattg gactgggcat
      301 accaggctgt tgtagcagcg gttgaagggg gcgcggtcga tgcctacggt cagcctattt
      361 ccgccaacag cctgaccggc gcggagttgg tctatgccga tttgcctgaa gcagccatct
      421 gattcataac acggaaggtc ggctgaaaaa cagtttcaga cgaccttcgc ataagggaaa
      481 agaaaatgtc tgaaccgatt attacccctg ccgccgttta tatttttggc ggcgtggtcg
      541 gcgcaggtta tctgttgggg atgcctgtcg atgcagtcgt gctgggcgca gtggcttcgg
      601 cggcggtaac gatgataagc gagccgaaaa gccgctggat ggtagtggcc tatatcgtta
      661 tcggcgggct tttgggtggc gcttttgccc caccgctgac gcatttctta atcggcgaag
      721 tagttggcag ccatccgtcg ctgggtgagc agcgcataca gtttgcacat gcctttgccc
      781 ccgtcgttgt cggcttgttg tggcaggtat tgttgagatt tgcccgcgct gtctggccat
      841 cgttcgaaaa gcatgcagac gatattgtgg agtggctgct gaatcttctg ccgtggagac
      901 cgaaaaaatg aaattacttt tgattctttc tgccgcccca tcactgattt cggcgtactt
      961 gatgatttgt cgtctgaacg ggcgcaaatg gagtgcattg tcgccggagg cgtgggcgta
     1021 tctcgccttt ttaggtggag cgatctatac cttttacata gtctttgctt acggactgac
     1081 gccgagctta ggcaaattta tgatggatat tgccgcctgt gtatatttcg gcagccgttc
     1141 ggtacgggtt gtcaaatggc ggcatcggca tggtttgaaa tagtttgcct gaaaaaagca
     1201 aaacgccgca aagtatgcag cttcgcggcg ttttcgtatc aaaactttat acgcagaatt
     1261 attgagatag ccgcctgtcg tttaggcggt tttttatttg aaaagaacat ggagaaccga
     1321 aatgcaagtt accaaaaatt tcagtctgcg cgagttgaca cgcagtgaaa ccgcgcgtcg
     1381 cgcaggtgta ccgaacgaac cgtcgcccgc cgacatggac aacatttatt acaccgcaca
     1441 gcagcttgaa aaaatccgcg aatacgtcgg tcgcccgatt atcgtaacaa gctgcttccg
     1501 cagcgagcgg gtaaacaaat tggtcggtgg cagcccgaca tcggcgcacc ggcacggact
     1561 tgccgccgat tgtgacgcat cgggtatgac ctcgccggca tttgccaaac tgttaatcaa
     1621 aatgcgtgat gaaggcgcgc tgaaattcga ccagttgata ctcgaatttc ccgagcgcgg
     1681 ggacggcgca tgggtacaca tcggtttccg acgcaacagc ccgcaacgca accagatact
     1741 gaccgcaacc aagaaaaacg gcaaaaccgt gtatctgccc gggctgcatc cttgaggtcg
     1801 tctgaaatgg atattttatt gaaatactgg aagccggtag gcgtattgct gctaatcgtc
     1861 ctgattttta ccgcatggca tttcgaccgt gccgaaaaat accgcatggg acgggaggct
     1921 gctgctgccg aaatttcgaa tcgtctgaaa gacggctata tcgagcaggc aaagcaggcg
     1981 cgttctgccg agcagaaggc cgctgccgcg tttgccgaac gacaaaccaa attagaagag
     2041 gaaaaacaaa atgctgaaaa aactgttgcc gctatgcgtc ttgagcttaa ccgcctgcgc
     2101 cactacgccg ccgcccaaaa tcgcaacaga aacctgcccg caaccactac cgccgccacc
     2161 gcatctgatg gcgcgtcaga ttcccaaggc tggctattat tcggacagtg cgctgaaaaa
     2221 tatgcaggac tggcagaaac agccgacgga catgcagccg atttaaggga atggcaggca
     2281 tatggtcatg cagtaagcag tcagcgtgcc gaataagcaa ccgcccgaac ctgtaagaaa
     2341 agattacagg ttcgggcggt ttcagcattt aatcgaataa gacggcgccg atgcgcgcca
     2401 gcacatcgtc caatacataa tcgggtacag tttctttaaa agaagccttg cggatttgcc
     2461 agtccagaga tttgagctgg tggcagtgga cattgccctg cgtttccgtt cctgcaccga
     2521 gtaaggttga aatcatgccg ctgcttcgtg cagccgctgc attcccctgt gaaatggggc
     2581 aggcaaaaac caatcccgtt gcgcggttga atgcttttgg agacagagcc agcgcaaacc
     2641 gcccgccctt gatttccttg ccgctggaag ggtcgaaatt caaatggaaa atatcgcctt
     2701 tgtcgggaat atacatttca gacgacctcg ttgccgacat catccaagat ttcccagcct
     2761 tctacgcgcg gcggggtttc ttccatttcg gcaagcaagt ctgccaagcg gaaacgtcgg
     2821 gcagcacgca cacggagttc gccgttatgt acttccgcta ccaaagcgtc gccgatttta
     2881 aaatccaatt gtttcagcat gtcggcaggc agtcggacgg ccgccgagtt cccccatttt
     2941 tggacacgca acataatctt cacctttatt gtatctacaa agtagataca tattaccata
     3001 aaatttcagt tgttcaaata cttgtgcaga atacgccaaa agccgtccga actgtttcgg
     3061 acggcttttg tactgtattt gcgccttcag gcaatatttt gttatccatt ttcaagatgc
     3121 aaaagctttc taattgcttg atgtcggatt tcggttgttt agggatacaa aaccaagtaa
     3181 actaaaactg tttgattgga aaatgctccg caaggagaaa attatgttca aaaaatcact
     3241 ttataaggct gctttggcgt atttcggcga ttgcgtggct gcccatattt cagaacagtt
     3301 ttgactgttt attcacaaat cagatgcctt tagggggttt gctttccata atgcaaccaa
     3361 aatttccaac tctctaaata ttgtgtcttt gcgttcttct tcgcgtattt tcataacaag
     3421 aggcgaaact gcattccaca attttatgaa gttggtgcaa tgcagccgtt taaacaaatc
     3481 ctcatccaat actcccgagt tgattgcgca cgcataaaac tcgtgccgat tgattacggt
     3541 aagtaaatgc cctctctctt tttcccattg tccgccctta ttttgataaa tttcatacag
     3601 gtcgatccgc gcgttgttgt cgtcaactgc cgtgccgcga atataaggcg aaatatgatt
     3661 gtcggcttct acaaattgtg catcttggta atcattcaaa ataacatcta atgtcgcctt
     3721 ttgctttgaa gttttcttat tgatgaatat tgtccccagt gcaatgacgg cggttactga
     3781 aacaacggtc agttgccaaa acatcagcca ttcggccgtc ccccataggt taaactgtac
     3841 gctgtgcctg aatatgtaaa ataaagtgaa tacagataca aacaaggagg ttagggcaat
     3901 tactgacaac caacctcctt gttttatttg tttccgcacc ttaccagcca ttaaaaccct
     3961 catttttgta tttcatcgta tttccttaat ggttgtaacc ttgcttgtcg atatctgtat
     4021 attaatgccg tgcgcccgtt ctttcaaggg gtgatttaaa aatcaggcat ccttgacatc
     4081 ctctcctgct taaaggcggg gggactcctg ccgtgtaaac aaatgccgtc tgaagggctt
     4141 tcagacggca taaaaaaacc gcctttgtgt aaagcggttg caaaaaagcc tttcaataaa
     4201 aatgccgtct gaatttcaga cggcattgtt gtcggatatg cctattcctt atccagccgg
     4261 cgcagggttt cggcgagctg tttgaagtcg gtttctcccg ccgccaaact gacaatcaag
     4321 tcgtcaagcc cttggtcggc ggcaatgctg atgccctgca aatccagata ggtcaacatt
     4381 gttaaaagcg cggtgcgctt gttgccgtcg ggaaaggcgt gggctttggc tatggcttgt
     4441 gcatagaggg cggcgatttc gtagatgtcc tcaaggtttt catactgccg ccagttggca
     4501 atccgcgaca atgcgccgtc caagcacgcc atatccgccc gccctttcaa acccgcttca
     4561 tccgccaata cggtttgatg gataagcgcg accagttcgc cgtctatcat ttgtcggcaa
     4621 gtgccttgac ggctttttga tgggttttgg caatgcggcg ggcggcggca agcagtacgc
     4681 gtttgcctgc ttcgccttta agctcgattt tgacggggcg tgtattttgg ttttgcatcg
     4741 ctgctccttg tctgtttgcg ggcattttag cttttttccg gcagcttggg aaatgccgtc
     4801 cgaaaacact tcagacggca ttcttctaat agtgtaatgc aatagttact ccaagatttt
     4861 tgtatatggc tgtttttatg gtaaattaaa ttttcataag aaataaatat atttaaatca
     4921 atgaaataaa atttagtcga atcccaccgt ttccgccaga aaacataacc gccctgaatc
     4981 agggcggttc ttttttgttc aag
//
LOCUS       DAWEWP010000138         4857 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWEWP010000138 DAWEWP010000000
VERSION     DAWEWP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.138"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(210..932)
                     /gene="rmpM"
                     /locus_tag="WGN53_001680"
     CDS             complement(210..932)
                     /gene="rmpM"
                     /locus_tag="WGN53_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ0140647.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPVVVVEQAPQYVDETISLSAKTL
                     FGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYVVA
                     NNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPDRR
                     VDVKIRSIVTRQVVPAHNHHQH"
     gene            1296..2246
                     /locus_tag="WGN53_001681"
     CDS             1296..2246
                     /locus_tag="WGN53_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ0140648.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2359..3093)
                     /gene="fnr"
                     /locus_tag="WGN53_001682"
     CDS             complement(2359..3093)
                     /gene="fnr"
                     /locus_tag="WGN53_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ0140649.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTYFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3298..4719
                     /gene="hemN"
                     /locus_tag="WGN53_001683"
     CDS             3298..4719
                     /gene="hemN"
                     /locus_tag="WGN53_001683"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ0140650.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTASV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1241 a         1330 c         1173 g         1113 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tccgaacctg caccccgatt ctgacgatag
       61 ccgtttcaga aaaccaccca aaaaaacaaa ccccgcaaaa gcgggggttt gtttcacatc
      121 atcgatgccg accgacgttt cacgaatatt gcaacagtac ctggtacaaa atccactaac
      181 ctcatgcatc ggcaagatat tgcctagcct tagtgttggt gatgattgtg tgccggcaca
      241 acctgacggg ttacgatgct gcggattttc acatccacac ggcggtcagg ttcgatacat
      301 gcaatcagag cctcacgttt tttggcttta gagactttcg cacccagttt ggcaacttcg
      361 gcttcacaaa cttgagtcat ttgcgcttga gattcgccca agccgacagc agaaattcta
      421 gaaacaggta cgccgttgct gaccaggttg tttgccacta cgtatgcgcg gcgttcggac
      481 agggcctgat tgtatttgtc agaacccata aagtcggtat ggccttcgac gcggacagat
      541 tggatattgg tttgacccag gcgttgcgcc aatactttca ggttgtcttg agcttcggcg
      601 cgcaatgaat ccttatcgaa accgaacagg gttttggcag acagggaaat ggtttcatca
      661 acatattgcg gagcctgctc cacaacgacg acaggagcgg gttcgggttc tggctcgggt
      721 tcgggggcag caaccgcatc gccgcattct acgcgacctt ggcttgcttt atcaaagtag
      781 gcgtttttcc agcattcgcc atagttgttg cgtacgattt cgttcgactg gccgcttacg
      841 gtgtaaccct gaacggacgc ctcgcccgca acagcagtgc cggaagcgag caatgcaacg
      901 aataatgcgc ttaatttcag ctgtttggtc attttattcc ctcattaaat ttgtacagca
      961 gatacagaag caactgctga attgccaaca tacaggccgc tgtagaaaca tggcggcaaa
     1021 tcggatattt cagtctccac tataaagaag cagggggagg actgtcaata ctcaacgcct
     1081 gaaaacaacc gtaaaacagc ctgtttcctg atagtctatc atgccgaaaa atcaattccg
     1141 tgtcattact ttagggggat tttttccata tcgcacgatt gccgttgcac aaaaacaaca
     1201 aacacaacgg caaagcccca taccgtaccg gcaagaaaat atgataaatt ataaacaatg
     1261 ttcagccacc cgacacagac ccacaccgac ccgccatgaa attacaacaa ttgaaatacg
     1321 ccttagaagt ttaccagcac aacctgaacg tttccgaagc ggccgaagcc ttgtttactt
     1381 cgcaacccgg catctccaaa caaatcaaat tgctggaaga agaaatcggc attcagattt
     1441 ttatccgcag cggcaagcgc gtggtttcgg tctcgcagcc gggcaaggtg gttttggata
     1501 ttgcggaacg tattttacgc gatgtacaga acattaaaaa tatcggcagc gagtttaccg
     1561 gacaggacag cggttcgctg acggttgcca cgacgcatac tcaggcacgc tatgccctac
     1621 ccttgattgt tgccgatttt gtgaaacgct atccgaaagt caacctgacc atcaaacagg
     1681 ggagccctgc cgccatcgca cgtatggtta cctcaggcga ggcggacttg gcgattgtta
     1741 cggaacggat agacgaccat cccgaactgg gaaaacttcc ctgctatgac tggactcatg
     1801 cggttatcgt accgaacgac caccccttgc tcgaatgcag aaaccccctc cgcattgaag
     1861 atttggcgag gtttccgctg attacttatg aatttgcatt caatgcgggc agcagcatcg
     1921 cgcgggcatt ttccaaagcc cgtttggaac agccggatgt cgcattggcg gcggcggata
     1981 cggacgtatt gaagacttat gtgcgcttgg gtttgggcgt gggactgatg gcgaaaatgg
     2041 cgtacaaccc ggatacggac ggcgatttgc agcttgtgga tgcggcacac ctgttcgagc
     2101 cgtcgccgac gtggattgct ttgcgcagcg atacttattt gcgcggatat gcctacgact
     2161 ttatccaagc gtttgcgccg cacctgacac gcgagaaggt ggataggatt ctgtacacgc
     2221 ccatcagcga ggatttttcg atttaggcgg ctgccggttt tcagacggca ctttgcggca
     2281 gatacaacaa acaggacaga tgttttcgtc tgccctgtgt ttattgagaa tgccgtctga
     2341 aatgttcgta cgggttaatc aaatggcgtg cgaacaaccg gataccatct tcttcaacac
     2401 ctgcagattg aggatcttga tgtgtttgtg ttcgacggaa atcaatcctt cctgatgaaa
     2461 tttagataat gtgcggctga cggtttcaag tttcagcccg agataactgc cgatttcttc
     2521 gcgggacatt cttaagatga agtcgttggc agcaaaacct cgggaataaa ggcgttggga
     2581 aaggttcaac agaaaggcgg caatccgctc ttcggcgcgc atattgccca acagcagcat
     2641 aacaccttgg tcgcgcacga tttcacgact catcatgcgg aaaaagtagg tacgcaggct
     2701 ggggatattc tgccccagtt cttcgatgtg ggtaaacggc agttcgcaca cttcgctgtc
     2761 ttccaaggcg accgcgtcgc aactgtgcac atgggaacag atgccgtcca tgccgatgag
     2821 ttcgcccgac ataaagaaac ccgttacctg atcgcggccg tcctgactgg cgacggttgt
     2881 tttgaagaag cccgaacgga tggcaaagag cgaggtaaag gcttcgccga cacagaacag
     2941 gtattcgccc tttttcaggc ggcggctttg acggatgacg gcatcgagtt ggctgagctc
     3001 gttgggcagc agcccgacag gcaggcagag ttcccgcaaa gaacaggaag aacacagcgt
     3061 tttcatctga tgtgtagtat tatgcgaagc cataccgtac ctttttgtgc gctttgcccc
     3121 atcatgatta tagtggatta aatttaaacc agtacggcgt tgcctcgcct tgtccttatt
     3181 taaatttaat ccactatatg tgcttattga cacatatcaa gacaggttta tcatactgtg
     3241 gcattctacc aaactatcca aaatttgact aacatcataa aaccgcacac acacaccatg
     3301 aaaatcattc agatacagaa caatcacaat gtaaacgatg accgccccga gtttgaccgc
     3361 gcgctgattg ccagcctgcc cgccagcggc ccgcgctaca cttcctaccc taccgccgac
     3421 cgtttccatg acggtttccg cgaaggcgaa tatatcaaag ctttacattt gcgcggtatg
     3481 ggcgcgttaa acaaaccgct ttccctttac attcacattc cgttctgcaa caccatctgc
     3541 tactactgcg gctgcaacaa aatcatcacc aaagacaaaa gccgcgccga tgcctacatc
     3601 gaatatcttg aaaaagaaat ggaactgctc gctccacatc tgaacggacg gcaccagctt
     3661 gcccaactgc acttcggcgg cggcacgccg acctttttga gcgacgaaca gatcgaacgt
     3721 gtcttccgca tgatacgcaa acatttcgag ttaatcccca ccggcgaata ctccatcgaa
     3781 atcgacccgc gcaaagtcag ccgcgacacc gtcctcatgc tcggcagact cggcttcaac
     3841 cgcatgagca tcggcattca ggatttcgac cccaaagtgc aggcagcggt caaccgcatc
     3901 caaagttacg aagaaaccaa agaagtcatc gatgcggcgc gcgaagcggg gttcaaatcc
     3961 gtcagcgtcg atttgattta cggcctgccg caccagactt cggaaagcat caaaaccacc
     4021 atcgataccg ttttgtcgct cgatcccgac cgcctcgccc tttatcacta cgcccacctg
     4081 ccgcacgtgt tcaaaccgca acgccgcatc gataccgcct ccgtccccga cagcgaagag
     4141 aagctcgata tgctgcaata ctgcgtccaa accctaaccg aacgcggcta cgtcttcatc
     4201 ggcatggatc atttcgccaa acctgacgac gaactctcca tcgccctcaa agaaggcttc
     4261 ctccagcgca acttccaagg ctattcgacc tacgcggatt gcgatttggt cgccatcggc
     4321 gtgtcgtcca tcggcaaaat cggcagcacc tattcccaaa acgaacgcga catcgatgcc
     4381 tactatgccg ccatcgacga aggcagactg cccatcatgc gcggttatca gctcaatcag
     4441 gacgacatcc tgcgccgcaa tatcattcag gatttgatgt gccgcttcgc gctcgactac
     4501 cggatttacg aaagtatgtt cggcatcccg ttcgaccgct acttcaaaga cgaactggcg
     4561 gatttggaaa aactcgccgg tttgggattg gtgcgcctga acagccacgg gctgaccgtt
     4621 accccgaaag gacgcttcct catccgcaac atcgccatgg tgttcgacta ccacctgcgc
     4681 cacaaggaaa ccaaggcgaa atattcgcaa acggtgtgat tgcgtctaac gtacaaatgc
     4741 cgtctgaaag gctttttcag acggcatttt gctgccggca ggataagtgt tttcaagaac
     4801 aggcggcggc atatcataac gttccgcacc tttgtgtccg accgttccga aaccaag
//
LOCUS       DAWEWP010000139         4838 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWEWP010000139 DAWEWP010000000
VERSION     DAWEWP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.139"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            97..903
                     /locus_tag="WGN53_001684"
     CDS             97..903
                     /locus_tag="WGN53_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ0140651.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            complement(961..2226)
                     /locus_tag="WGN53_001685"
     CDS             complement(961..2226)
                     /locus_tag="WGN53_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ0140652.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLNPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEAIIKEFQTKGFITNE
                     AGYGKEITNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            2633..3343
                     /locus_tag="WGN53_001686"
     CDS             2633..3343
                     /locus_tag="WGN53_001686"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ0140653.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPKTE
                     AGLALLKSLGIPLAVVTNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            3416..3877
                     /gene="recX"
                     /locus_tag="WGN53_001687"
     CDS             3416..3877
                     /gene="recX"
                     /locus_tag="WGN53_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ0140654.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IDVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            3951..4112
                     /locus_tag="WGN53_001688"
     CDS             3951..4112
                     /locus_tag="WGN53_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140655.1"
                     /translation="MSEQPEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            4265..4747
                     /locus_tag="WGN53_001689"
     CDS             4265..4747
                     /locus_tag="WGN53_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0140656.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
BASE COUNT         1273 a         1417 c         1101 g         1047 t
ORIGIN      
        1 aaagacagtt ttcagacagc aaatccgtct tcacacgata cctattttgt tataacataa
       61 caaaatcttt gacccacacg agacaaaggc tgcaccatga agaaaacatt gacactgctc
      121 gccgtttccg ccctatttgc cacatccgcc cacgcccacc gcgtctgggt cgaaaccgcc
      181 cacacgcacg gcggcgaata ccttaaagcc gacttgggct acggcgaatt tcccgaactc
      241 gaacccatcg ccaaagaccg cctgcacatc ttcagcaaac cgatgcagct ggttaccgaa
      301 aaaggcaagg aaaacatgat tcaacgcggc acatacaact accagtaccg aagcaaccgt
      361 cccgttaagg acggcagtta cctcgtcatc gccgaatatc agcctacttt ctggtcaaaa
      421 aacaaagcag gctggaaaca ggcgggcatc aaagaaatgc ctgacgcaag ctattgcgaa
      481 caaacccgaa tgttcggcaa aaacatcgtc aacgtcggac acgaaagcgc ggacaccgcc
      541 atcatcacca aaccggtcgg acaaaacttg gaaatcgtcc cgctggacaa tcccgccaac
      601 attcacgtag gcgaacgctt caaagtccgc gttctgttcc gtggcgaacc gctgcccaat
      661 gccaccgtta ccgccacctt tgacggcttc gacaccagcg accgcagcaa aacgcacaaa
      721 accgaagcac aggctttctc cgacagcaca gacgacaaag gcgaagtgga catcatcccc
      781 ttgcgccaag gcttctggaa agccaatgtc gaacacaaag ccgacttccc cgatcaaagc
      841 gtgtgccaaa aacaggcgaa ctactcgact ttaaccttcc aaatcggcca ttcgcaccat
      901 taatcccgcc cgcacaaaaa tgccgtctga aggcttcaga cggcattttt tgttcaaaca
      961 tcaataccaa ccgcgcagtt tcatcgcttt ttcaacacgg cggatactca tcatgtaaga
     1021 cgcggttcgc aaatcgacat catactcttg cgccaagttc catatatcgc ggaacgcacg
     1081 gcgcaggacg acggtttctt tttcctgaac ttcgtcaaac tcccaataat agccttgcag
     1141 attttgtacc cattcaaaat aagataccac cacgccgccg cagttcgcca aaatatcggg
     1201 cacgaccaat acgccgtttt gacgcaggat aacgtcggct tcgggcgtag tcgggccgtt
     1261 cgcgccttcg accacgattg tcgcgcggac tttaccggca ttttcagaag tcagttggtt
     1321 ttccagtgcg caaggggcaa gtacgtccac ttccagagcc aaaagttcgg cgttggtaat
     1381 ttccttgccg tagccggctt cattggtgat gaaacctttg gtttggaatt ctttgatgat
     1441 tgcttccata tccaagccgt tttcgttgta aatggcaacg tcaacagtag aaaccgcaac
     1501 aactttcgcg ccggattgat gcgcgtaata acctgtgtgg taaccgacat taccgaagcc
     1561 ttggatggcg taagtggcac ccttcacgtc tttgcccagt ttttccaagg cttggacggc
     1621 ggcgaggttc acgccgtaac cggtagcctc ggtacgcgcc aaagagccgc cgaactcaac
     1681 cggtttaccg gtaaatacgc ccggtgcaga atgtttcacc acgttttcat aggcatcaac
     1741 catccaagac atgattttgc cgttggtgtt cacatcaggt gcgggaatat cgattttctc
     1801 gccgatcagc ggagaaatgg cttcggaata agcgcgggcg atgcgttcca gttccgcctc
     1861 ggaataatcg cgcggattca aggtaacgcc gcctttgccg ccgccgtaag gaatacccgc
     1921 aacgcagcat ttgatggtca tccaaatcga cagggctttg acttcgtcca gattcactcc
     1981 gggatggaag cgcacgccgc ctttataggg gccgacggcg ttgttgtgtt gcgaacggta
     2041 gcccgtgaag gttttgaccg tgccgtcgtc gagtttgacg gggaaattga cttccaacac
     2101 gcgggtcgga ctcttcagga tttcataaac ggccggatcg gttttcagcc ggtcgcaggc
     2161 ggttttgacc tgtttgcgcg cgatttcaaa cggattgagg gtttcttttg caagggcttc
     2221 agacattttg cttccttttc acaaaaagat tttcaggggc gtgcatttcg ctcattacta
     2281 taaccaattt cccgacaaaa tgtaatagcg tgtagtcggc ataagcccga tttgattaat
     2341 ctatatatga ttttattttt caagccgcac ggaatcagcc cggactccgc ctgaaaaagc
     2401 agcgcgcgcg tccgaaaaat aaatgtcaaa ttaaataaag atataggcat ccttttattt
     2461 tttaaaaatt taattggaac atcgctggga tttcaaaccc ttgcccgatt ccgccccgaa
     2521 atccgaaaac accgcctgca aaacctgttg cgattgttaa caatctatac attagaagcc
     2581 ctgtgcaaac gatgttaaaa taaacccttt caacccgaca ggaaatcaga ttatgaatgc
     2641 agccatcgaa cacgtccaag ccgtcgcctt cgatttggac ggcacactgt gcgattccgt
     2701 ccccgacctt gccgccgccg cagaagcgat gttggagcaa ctcggtatga aaccgctgcc
     2761 tgcaaaagtg gtcgaaagct atgtgggcga cggcatcggc aaactggttc accgcgtcct
     2821 caccaacgac cgcgaccgcg aagccgattc cgaactgtgg gaaaaaggtt tcgtatccta
     2881 tatgaaatac taccgcgacc atttgagcgt cttcacccgc ccctatccca aaaccgaagc
     2941 cgggctggca ttgctcaaat ctttaggcat cccgctcgcc gtcgttacca acaaaaacga
     3001 aatccttgcc gtcgaactgt tgaaacagct cggacttgcc gactacttca gcctgatact
     3061 cggcggcgac agcctgcccg agaaaaaacc cagccccctg ccgctgcggc atgccgccga
     3121 agttttgggt atcgatgttg caaacatggt tatggtcggc gactcgcgca acgacatcat
     3181 cgccgccaaa gccgccggct gcctgagcgt cggcgttacc ttcggttacg gcgatatgac
     3241 gctgctctcg caagacgatg cgacccgccc cgactggatt atcggctcgc tgcccgaaat
     3301 ttacgaaaac ctgcaaccgc agaaaaataa agacgaagaa taaaacaatc tgttcagacg
     3361 gcatcggaat ccgtttccca tgccgtctga atctttccgt gccgaaaccg ccgccatgaa
     3421 accgcaaaaa tccctacgcg cccgcgcgat ggacatcctc tcgcgccaag aagtcagccg
     3481 catcggtctg aaacgcaaac ttgcaccgca cgccgaaagc gaagaagagt tggaaaacgt
     3541 gttaaacgaa tttgccgaac gcaactggca gtcggatttg cgctatgccg aagcctatat
     3601 ccgcagcaaa agccgcaaac acggttcatt gaggctgaaa caggctttgg cgcaacaggg
     3661 catagatgaa gaaaccagcc gcaacctgct tcccgaccgc tcaagcgaaa aacaggccgc
     3721 catagacgtg ttgcgtaaaa aattcaaaca ccccgccgcc gaccttaaag aaaaacaaaa
     3781 acaggcgcgt ttcctcgcct atcgcggatt cgatgccgat accgttcaga cggcattgaa
     3841 acacgcttgg gacgaaaatt gggaagacgg ctgctgacct tccttcttga atctttttgc
     3901 acggcaacat aaccttacgc ccgtttccaa cttttccgat tgagaacaaa atgtccgaac
     3961 aacccgaaaa aaatcataac cccctccttg aggacgaacg caagaacccc gtgtaccgca
     4021 tggggcaggc agttgccgga ttcatgctcg tcgtttgggt cgccgtattg gcactcgtgt
     4081 ttttcctggt cttccgtttt tggctttcct aaacaaaatg ccgtctgaag gcttcagacg
     4141 gcatcggcgg tccatttcgg cagactatcc catcatagct ttttttagct tgaattccac
     4201 ttccccattc cctaaaattt ccccgcaccc atttcaaaac accctttctt aaaacaggta
     4261 caccatgaca caacaacgcc aactgccttc ccacgaactc attatgtccg aactgatgat
     4321 gccggacacc gccaatttca gcggcaacgt acacggcggc gaactcctgc tcctgctcga
     4381 ccaagtcgcc tattcctgcg ccagccgtta cagcggcaat tattgcgtta ccctgtcggt
     4441 tgacaaagtc ctgtttaaag aacccatcca catcggcgat ttggttacct tttacgccgc
     4501 tgtcaactac acgggacgca cctcgatgga aatcggcatc cgtgtcgaag cacaaaacat
     4561 ccgcaccggc gaaatccgcc acaccaacag ctgctacttc acgatggttg cggtcaaaga
     4621 cggcaaaccc gtccctgtcc ctccgctgga aatcctgacc gaccgccaac gctgccgcta
     4681 cgaaaaagcc aaaaaacgca gagacatcag cctgcaagcc tctgaagaca tgtcctgcgg
     4741 ctgctgacgg cggactatgc cgtctgaaag acaggcacat cgcgccatcc gtttccattg
     4801 caaacggatg aaatcaagca aatatagtgg attaaatt
//
LOCUS       DAWEWP010000140         4831 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWEWP010000140 DAWEWP010000000
VERSION     DAWEWP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.140"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..143)
                     /locus_tag="WGN53_001690"
     CDS             complement(<1..143)
                     /locus_tag="WGN53_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0140657.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LWT"
     gene            384..1538
                     /gene="obgE"
                     /locus_tag="WGN53_001691"
     CDS             384..1538
                     /gene="obgE"
                     /locus_tag="WGN53_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ0140658.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ENVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDSPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEQAAAKAEIAEQQPKTDTGVFKPE"
     gene            1621..2670
                     /locus_tag="WGN53_001692"
     CDS             1621..2670
                     /locus_tag="WGN53_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0140659.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3358..4779
                     /locus_tag="WGN53_001693"
     CDS             3358..4779
                     /locus_tag="WGN53_001693"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ0140660.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGRGRPGWHIECSAMSENLFG
                     NTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNNAQL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARSDKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
BASE COUNT         1411 a         1183 c         1135 g         1102 t
ORIGIN      
        1 atcgtccaca ggacaatggt ggtcagaatg ccgatgaaac cggcaacggt cagccagtgc
       61 agcgcgcgtg tcaacgggtc gtaaacggca ggttggtttg tgttcataaa aaacttatta
      121 tatgtaacgg tttgaacgga cattccgtcg ggtcggaatg tcaaaaaggc gcgattgtac
      181 caaagaagtt ggacataatt ttgtttgcag gctgaagatt tgcttaaaaa ttcattaaga
      241 tgggcggaac aaatagtttg ggtacggcgt gttgaaatac cgcttatccc ttataatagc
      301 gtccgaaatt ctgttcagac ggcatcaaaa cacacggtaa tccgtctgaa cccccatttg
      361 aaaccaacaa acaagagcat tgaatgaaat tcatcgacga agcaaaaatc gaagtcgccg
      421 caggcaaagg cggtaatggc gcaaccagtt tccgccgcga aaaattcgta ccgcgcggcg
      481 gtccggacgg cggcgacggc ggcaaaggcg gcagcgtttg ggcagaagcc gacgaaaaca
      541 ccaacaccct cgtcgaatac cgcttcgtta aacgctacca agccaaaaac ggcgaaaaag
      601 gccacggttc cgaccgttac ggcgcaggtg cggacgacat cgtcctcaaa atgcccgtcg
      661 gcacccttat ccgcgacctc gacaccggcg aaaacgttgc cgacctcact tatcacggac
      721 agcgcgtctg ccttgccaaa ggcggcaaag gcggcttggg caacatccac ttcaaatcct
      781 ccgtcaaccg cgccccaaaa caatccacgc ccggcgaaga aggcgaagcc cgttccctgc
      841 aactcgaact caaagtcctc gccgatgtcg gcttattggg tatgcccaac gccggcaaat
      901 ccaccctgat taccgccgta tccgccgcac gccccaaaat cgccaactac cccttcacca
      961 ccctgcatcc aaacttaggc gtggtacgca tcgacgaaaa ccacagcttc gtgatggccg
     1021 acatccccgg cctgattgaa ggcgcggcag aaggcgcggg tttggggcat cgtttcctca
     1081 aacacttatc gcgcaccgga ttattgctgc acgtcgtcga tttggcgccc ttcgacgaaa
     1141 ccgtcaaccc ggccgaagaa gcacttgcca tcatcaacga attgcgcaaa tacgatgaag
     1201 aactttacgg caaaccgcgc tggctggtgc tgaataaact cgacatgctt gacgaagaag
     1261 aagcccaaac gcgcactgcc gccttcctcg aagccgtcgg ctgggactcc cccaaacccg
     1321 acgaccgttt ccaatttgac atggaaaccc cgcgcctctt ccaaatcagc gcgctgaccc
     1381 accaaggcac gcaggaattg gtacaccaaa tcaaccaata cctgaccgag aaaaaacgta
     1441 tcgaagccga aaaagcagaa gcggaacagg cagcggcaaa agccgaaatt gccgagcagc
     1501 agccgaaaac ggatacgggc gtgtttaagc cggagtgaaa aaagaaaggg tttcagacga
     1561 tatttgttct acaaaaggtt gtttgaaaca attttaaagc aagattgaaa tggattaaaa
     1621 atgtcatcta accaatatat ccagtcgctt gagttgacaa actttaccgt tcttccaaac
     1681 gatacttttg aattttctga aaatctcaat gttattgttg ccgaaaacgg ttgtggcaaa
     1741 acacatttgt taaaaatttt atacagcctg ctagaagtaa catctaacac caaaaacaga
     1801 ttactgaaaa cagaattgca aaaaagtttt gccgataaat tactgaatgt tttccgtccc
     1861 gattccttgg gcaggttgag caaacgtttg caggggcgcg ggcgtacgga aatcgtttta
     1921 aaactgcaaa acggcactac gcatagtagt ctgaattttt ccagtaactc atcatcgcaa
     1981 gtcaatgttc aaagcatcgg attgaaagaa aatgaatata ctccgacacc tatcttctta
     2041 cccagccgcg aattaattac actttgccct tggttcactt ctttgtatca aaaccaaagc
     2101 atccctttcg aagaaacttg gtttgatgcc tgtatgcaat taaatcaccc gcttgccaaa
     2161 ggccccagag aaacaaaaat tcgagaattg ctcgaaccta ttgagaacgc gatgggcgga
     2221 aaagtatctg aagaacaagg aaggttttat ctatcgctga caaatacggg cggtaagatt
     2281 gaagcacctt tggtagcaga gggtttgcgg aaatttgtca tgattgcccg gttaatcgca
     2341 acaggggcat tgctggataa aggctatcta ttttgggatg aaccggaagc taatctgaat
     2401 cctaaattga tcaaagtggc tgcccgtatt atttggtctt tatctcaaca gggtattcag
     2461 gtttttattg caacacactc tttattctta ttgagagaat tggaactttt gaagcttgaa
     2521 aataacacgg atttacctgt acgttttttc tcgttgattg cttccgatga tggaacaaaa
     2581 gtggagcaag ggaacagtat taatgatttg aacacattga tattgttaga tgaaaacatt
     2641 atgcaggcag acaggtatct gaatatttga ggttaagcta tgactacaat cacagaaaag
     2701 agattaacct ttgcttttcc tgaagattat tacgttacca agtatgacga atgggaacat
     2761 tataaaattt ttcagaacag ttgtaattta cgtaataaaa ttgatacaaa tgaaaaggga
     2821 aagaacggga taaatcaaag tgtagatgac gataatggca gctcaggtgt agatataata
     2881 gcccttcatg aatcgacact ttggctaatc gaaattaaag attattaccg acttgggctt
     2941 gagccaaatg cccaatcaat agatgaaaaa ctttctgacc taccatattt aatcgcaaga
     3001 aaaattcgag attcattggc aggattggtt tctgctaaat ttaaagcaga aaaacaggaa
     3061 gaaaaagatt tttctcgctt ggctttagat tgtaacgaaa taaaaatagt ccttcatatt
     3121 gaaatgccta gtataagaag taaactttat ccctcgtcat cagatttagc aaacttatta
     3181 aaagataagt ttaaattatc agaatttaca aaaaattttg caaactgtta tgcagaacca
     3241 attttcacaa atatcagcca tattaataat ccgcaactac gtaatgttcc ttggtccgta
     3301 tctacaggaa cagaacagaa attatcatcc gagcaacaaa gattaatcca tagtcccatg
     3361 accaccatct acaacaccct tacccgccaa aaagaaccct ttgcccccat cgaccctgaa
     3421 aacgtgcgta tgtacgtttg cggcatgact gtttacgact attgccactt aggccatgcc
     3481 cgtgtgatgg tggtgtttga catgattgcc cgttggttgc gcgagtgcgg ctatccgctc
     3541 acttatgtac gcaacatcac cgacatcgac gacaaaatca ttgcccgtgc ggctgaaaac
     3601 ggcgagacca tcggcgagct gactgcgcgt ttcattcagg ctatgcacga agatgccgat
     3661 gctttgggcg tgttgcgtcc cgatatcgag ccgaaggcga cggaaaacat tccgcaaatg
     3721 attgcgatga ttgaaaccct gattcaaaac ggcaaggctt atcctgccgc caacggcgac
     3781 gtttactacg ccgtgcgcga gtttgccgct tacggccaat tatcgggcaa atcgttagac
     3841 gacctgcgtg caggtgagcg tgtggaagtg gacggtttca aacgcgatcc gcttgatttt
     3901 gtgttgtgga aagcggcgaa agcaggcgaa ccggcgtggg aaagcccttg gggcagggga
     3961 cggccgggct ggcacattga atgctccgcc atgagtgaaa acctgttcgg caataccttc
     4021 gacatccacg gcggcggcgc ggatttgcag tttccgcacc acgaaaacga aattgcccaa
     4081 agcgtcggcg cgacggggca tacctgcggt catcatcacg cgcaaaccca ccacgggcaa
     4141 agcatcgcca gccacgtcaa atattggctg cacaacggct ttatccgcgt ggacggcgaa
     4201 aaaatgtcca aatcactggg caacttcttc accatccgcg aagtgttgaa acaatacgac
     4261 cccgaagtcg tgcgcttctt catcctgcgc gcccactacc gcagcccgtt gaactattcc
     4321 gacgcgcatt tggacgacgc aaaaggcgcg ttgacgcgtt tgtatacgac tttgaaaaat
     4381 acgccgcccg ccgatccgat gccgtctgaa gccggcgatg actacacgcg ccgcttttat
     4441 gttgcgatga atgatgattt tgatacggtg aaagcggtgg cggtgttgtt cgaacttgcc
     4501 ggcgaagtga acaaaaccaa taacgcgcaa ctggcaggct gcctgaaagc cttgggcggc
     4561 atcatcggac tgctgcaacg cgacccgacc gagttcctgc aaggcggcgc ggcttcagac
     4621 ggcctctcca acgaagaaat cgaagacttg attgcccggc ggaaacaggc gcgctccgat
     4681 aaaaactggg cggaatccga ccgcattcgc gaccttctga acgaacacaa aatcattttg
     4741 gaagacaacg ccggcggcac gacttggcgg cgcggttgaa atccatcgaa aagggtaaat
     4801 gtgccgcatt ttgccctttt ttgttcagac g
//
LOCUS       DAWEWP010000141         4776 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWEWP010000141 DAWEWP010000000
VERSION     DAWEWP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.141"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            214..993
                     /gene="pgeF"
                     /locus_tag="WGN53_001694"
     CDS             214..993
                     /gene="pgeF"
                     /locus_tag="WGN53_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ0140661.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1045..1524
                     /locus_tag="WGN53_001695"
     CDS             1045..1524
                     /locus_tag="WGN53_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140662.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1526..2524
                     /locus_tag="WGN53_001696"
     CDS             1526..2524
                     /locus_tag="WGN53_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ0140663.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPSKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            2645..3064
                     /locus_tag="WGN53_001697"
     CDS             2645..3064
                     /locus_tag="WGN53_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140664.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAIIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3140..3655
                     /locus_tag="WGN53_001698"
     CDS             3140..3655
                     /locus_tag="WGN53_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0140665.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLYLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3739..4623)
                     /locus_tag="WGN53_001699"
     CDS             complement(3739..4623)
                     /locus_tag="WGN53_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ0140666.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGSQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARKREGKKLGE
                     HLLQRLEAMEEIIDALSELFPTLLETHKEKIRARLVEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1065 a         1262 c         1337 g         1112 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactatat aaagctcgga gaaggtatat aagggcatca
       61 ttcccgcacc tgcaaaaatc cgaacccgaa cgtttgaaaa caatcccgaa tctccgaatt
      121 cccgcctgtg cgggaatgac gaaaaaacaa acatccattt gccccgaagg cagttaatca
      181 accctttccg ccacacacct attccaatat ccaatgaaaa ccatcacaga aaccctaaat
      241 cttgccccga aaggcaaaaa cttcctgacc gccgattggc ccgcgcccgc caatgtgaaa
      301 accctgatta ccacgcgcaa cggcggcgtg agccaaggtg cgtatcagag tttgaacctc
      361 ggtacgcacg tcggcgacaa tcccgaagcc gtgcgccgca accgcgaaat cgtgcaacag
      421 caggtcggat tgcccgttgc ctacctcaat caaatccaca gcaccattgt cgtcaatgct
      481 gccgaagcat tggacggcac gcccgatgcg gacgcttccg tggacgacac gggcaaggtt
      541 gcctgtgccg tgatgaccgc agactgcctg cctgttttgt tttgcgacag ggcgggtacg
      601 acggttgccg ccgcacacgc gggctggcgc ggtttggcgg gcggcgtatt gcaaaacacc
      661 attgcagcga tgaaggttcc gcctgtcgaa atgatggcgt atctcggccc cgccatcagt
      721 gcggatgcgt ttgaagtcgg acaggacgtg tttgatgcgt tctgcacgcc catgcccgaa
      781 gccgccgccg catttgaagg cataggcggc ggcaaattcc ttgccgacct ttacgcgctc
      841 gcccgcctga ttctgaagcg cgaaggcgtg ggcggcgtgt acggcggcac gcattgtacg
      901 gttttggaac gggatacttt cttttcctac cgccgcgacg gagcgacagg gcgtatggcg
      961 agcctgattt ggctggacgg caatgccgtc tgaacacgcc gctgatataa tctaccgact
     1021 ttgtgctttt gagaaaggca agccatgaac aaactgtttc ttactgccgc agtgctgatg
     1081 ctgggcgcgt gcggtttcca cctgaaaggt gcagacggca tttctccgcc gctgacctac
     1141 cggagctggc acatcgaagg cggacaggca ttgcagtttc ctttggaaac cgcgctgtat
     1201 caggcttcgg gcagggtgga cgatgctgcc ggcgcgcaga tgaccctgcg tatagacagc
     1261 gtttcccaaa acaaggaaac ctacaccgtt acccgtgcgg cagtcatcaa cgaatatctt
     1321 ttgatattga cggttgaagc gcaggtattg aaacgcggcg agccggtcgg caaaccgatg
     1381 accgtgtccg tccgccgcgt ccttgcttat gccgacaacg agatcttggg caaacaggaa
     1441 gaggaagcgg cattgtgggc ggaaatgcgg caggatgccg ccgaacagat tgtccgccgc
     1501 ctgacctttc tgaaggcgga atgacgtggc ggcacatatc ggacgcattg atacggacgc
     1561 gcctttgaaa cccctgtacg tcatccacgg cgaggaagaa ctgttgcgta tcgaggcatt
     1621 ggacgcattg agggcggcgg cgaagaaaca aggttacctt aatcgggaag tttatacggc
     1681 agacaatgct ttcgattgga acgagctgct gcaaaccgca ggcagtgcgg gtctgtttgc
     1741 cgatttgaag ctgttggaac tgcatatccc taacggcaag cccagcaaaa ccggcggcga
     1801 ggcgttgcag gattttgccg cccgcctgcc ggaagatacg gtaacgctgg ttttactgcc
     1861 caaactggag aaaacccagc tccagtccaa atggtttgcc gcattggcgg caaaggggga
     1921 agtgtgggaa gccaaaccgg tcggcgcggc ggctttgccc caatggatac gcggacggct
     1981 ggacaaaatc ggtttgggta tcggggcaga cgcattggca ctgtttgccg agcgcgtgga
     2041 aggcaatctg ttggcggcac gtcaggaaat cgacaagctc gcgctgctgt atccgaaagg
     2101 gcataccgtc aatatcgatg aggcgcaaac cgccgttgcc aacgtcgccc gcttcgacgc
     2161 gttccaactg gcaggcgcgt ggatgaaggg cgatgtcctg cgcgtatgca ggcttttgga
     2221 cggattgcgg gaagagggcg aagaaccggt gctgttgctg tgggcggttg ccgaagacgt
     2281 gcggacgctg atccggcttg ctgccgccct gaagcagggg cagagcatcc aatccgtccg
     2341 caacagcctt aggctttggg gcgacaagca gacgctcgca ccgcttgcag tcaagcggat
     2401 ttccgtcgtc cgcctgcttg acgcgctcaa aacctgtgcc caaatcgacc gaatcatcaa
     2461 aggcgcggaa gagggcgacg catggacggt attcaaacgg cttgtcgtgt cgctggcgga
     2521 ataaagcggt aatccccaaa atccgaaaat actgtaaaat accgttaatc ctgaaaagta
     2581 ttcaccaatc cgtccgaaaa catttcagac ggcacgacca cctcaataaa ggaacattaa
     2641 ccctatggac aataagacca aactgcgctt gggcggcctg attttactga ccaccgccgt
     2701 tttaagcctc attatcgtat tgattgtcga ttcctggccg cttgccatcc tgcttgccgc
     2761 catcatcgtc gccgccgctg cgggcggttt tgtttggaca tcccgccgac agcaacgcca
     2821 gtttatcgaa cgcctgaaaa aattcgacat cgatcccgaa aaaggcagaa tcaacgaggc
     2881 aaacctgcgc cgtatgtacc acagcggcgg acaacaccag aaagatgcga ttaccctgat
     2941 ctgcctgtcg caaaaatgtt cggtggacga ggcgcacgct atgttcaaaa aacgcccgac
     3001 acgtcaggaa atcaatcaaa tggcggcaaa acagtcgcgc ggtcagaaac gtccgcaccg
     3061 ttaaccgccg caaggcatct ttgcataaat gccgtctgaa gcctgttggc gtttcagacg
     3121 gcatattctg attgaaaaga tgatgatact gaaaaccgcc ccgctcaaac gccgctttgc
     3181 cgccatgctg tatgagatgc tgctggtcgg tgcggcaacc tgtttggcag cattgtttgc
     3241 cggtattgcc gccatttttc tgaatcccgt ttctatcgcg gtttctgcat tggtaacgag
     3301 tatcctgata atgggatcat ggtggcttta ttttcgcgcc aactggcacg gtcaggggca
     3361 gaccttggcg atgaggacat ggaaaatcgg cttgtgcgac cttaacggca tacagccgtc
     3421 tttgtacctg ttgcgcctgc gctttatttg ggcgtgcata ttcatcgtct tcattcctat
     3481 gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg cggccggcgc
     3541 ggcattgatt tggctgattt taccgtgggg gttcgcactg ctgaatcccg accggcagtt
     3601 tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc cttaagcctt
     3661 tataccgcaa aggtttcaaa ctgaaaaaat gccgtctgaa agggctttca gacggcattt
     3721 gcttatcggg gaaaccgatt attcgatatt ctgcacttgt tcgcgcatct gttcgatcag
     3781 gactttcagc tcgaccgagg cttgggtgca ttcggtagcg atggatttgc tgcccaaagt
     3841 gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgtttgccgc tgctgccttt
     3901 gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgtaggcggc tgaactcttc
     3961 gtcgatgtcg gatttttgga taaagagggc aaattcctgt tgcaggcggt cgttgtcgat
     4021 gctgccgacc gcttcgacga ggcgggcgcg gattttttct ttatgtgttt ccaacagggt
     4081 aggaaagagt tcgcttaatg catctatgat ttcttccata gcctcaaggc gttgcagcag
     4141 gtgctcgcct aatttcttac cttcccgctt gcgtgcggca gtaaagtctt ttaacgcttt
     4201 ttcggtcagt tcggtaatgc tttttgccaa ttcttccgta ttttcccttt ggcttgccaa
     4261 tacgccgggg aaacgcagga tgtcggcaac gcccagtttt gccaaatcgt gatgcttgcg
     4321 gaggtctttg ttgatttcgg caagctgtcc gaccaagtcg cgattcagtt ccaaggactg
     4381 actgccgttt tccgcatctt gaatttggat tttgcattcg actttgccgc gtgcgatatg
     4441 ggatgaaatt ttctcgcgga taccgctttc caaatagcgc aaatcgtcgg gcatcctgat
     4501 ttgaatatct agaaaacggt ggttgaccgc acgcaggtcg agattgatgc gtttgctgcc
     4561 gcactctgcc gccgcgttgg caaatccggt catgctgtgg atgtggatat ttccgctgct
     4621 catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt atggcggcaa
     4681 gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc acgcgctttt
     4741 gttgctgcaa ggaagggaaa tgtatagtgg attaac
//
LOCUS       DAWEWP010000142         4724 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWEWP010000142 DAWEWP010000000
VERSION     DAWEWP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.142"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            297..995
                     /locus_tag="WGN53_001700"
     CDS             297..995
                     /locus_tag="WGN53_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0140667.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1052..2056)
                     /locus_tag="WGN53_001701"
     CDS             complement(1052..2056)
                     /locus_tag="WGN53_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ0140668.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2126..4534
                     /locus_tag="WGN53_001702"
     CDS             2126..4534
                     /locus_tag="WGN53_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ0140669.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
BASE COUNT         1141 a         1439 c         1243 g          901 t
ORIGIN      
        1 ttgaaagggg tgtaaccctt ccgaaccagg gcaacgcata gggcggtgct ttatgtgccg
       61 tcctgtgtgt tggaacatca ttagttcccc aacagccgta tccgtctggg gcgacggcgc
      121 gtattgtacg acagaaaccg cttcagacgg catcgggttt gatgccgtcc gaagcggttt
      181 ccccaaacga aaacgtcctt ttccggaggc aaactgtatc aacacgcgga aatgcaataa
      241 aatagccgga ttccgattga tttccaacat ctgtttccaa catcacggag agccgtatga
      301 aatccaaaca cctcgccctc ggcgttgccg ccctgttcgc acttgccgcg tgcgacagca
      361 aagtccaaac cagcgtcccc gccgacagcg cgcctgccgc ttcggcagcc gccgccccgg
      421 cagggctggt cgaagggcaa aactatactg tccttgccaa cccgattccc caacagcagg
      481 caggcaaagt cgaagtcctt gagtttttcg gctatttctg tccgcactgc gcccacctcg
      541 aacctgtttt aagcaaacac gccaagtctt ttaaagacga tatgtacctg cgtaccgaac
      601 acgtcgtctg gcagaaagaa atgctgacgc tcgcacgcct cgccgccgcc gtcgatatgg
      661 ctgccgccga cagcaaagat gtggcgaaca gccatatttt cgatgcgatg gtcaaccaaa
      721 aaatcaagct gcaagagccg gaagtcctca aaaaatggct gggcgaacaa accgcctttg
      781 acggcaaaaa agtccttgcc gcttacgaat ctcccgaaag ccaggcgcgc gccgacaaaa
      841 tgcaggagct gaccgaaacc tttcaaatcg acggtacgcc cacggttatc gtcggcggca
      901 aatataaagt cgaatttgcc gactgggagt ccggtatgaa caccatcgac cttttggcgg
      961 acaaagtacg cgaagaacaa aaagccgcgc agtaagcccg tttgaaaaat gccgtctgaa
     1021 acttggtttt cagacggcat tttgattggg tttaaaacgt aaagcccgtt tccagttctt
     1081 catcgccgac cagttcgacc aagagcgcgt agagcggggc gagttcggca taacggcgcg
     1141 atacgcggcg caggtaattg aggaaacgcg ggatttcggg acggtatttg tctttgccgt
     1201 cacggtagta caggcgtgcg aagatgcctg caaccttcaa gtgccgctgc acgcccatcc
     1261 attcgaacca gcggtaaaac tcgtcaaacg cttcggggac gggcaagccg gcagcccgcg
     1321 ccttttccca gtagcggata accaagtcca agacaaattc ttcttcccat tcgataaagg
     1381 catcgcgcaa cagcgacacc aaatcgtagg aaatcgggcc gtaaagcgcg tcttggaagt
     1441 ctaaaacgcc cggtctgccg cgcgtcagca tcaggttgcg gacgataaag tcgcggtgca
     1501 catagacttt gggctgcgcc aacaggggcg gcagcagcgt atcgacggtt tgctgccaaa
     1561 gttggcgttg tttgaatgtt aattcgcgcc ccaattcttt tgcgacaaac cattccggga
     1621 acaggttgat ttcgcgcagc atcgtttcac ggtcatattc gggcaaaacc ccttcacggc
     1681 ttgccttctg caattcgacc agctcgccga ttgcctccaa aagcagggct ttgtgcgccg
     1741 cttcgccctg ctcctgaagc atcgcggtca aaaacgtcgt attgcccaag tcgttcaata
     1801 ccacaaaccc caaatccgtg tccgcgtgca atacctgcgg tacattgacc atgtcaaaca
     1861 gtttctgcac tttcaaataa ggtgcgacac tcatcttgtc gggcggtgca tccatgcaga
     1921 cgacactgct gccgtctgaa aacgttgcac ggaaatagcg gcggaaatca gcatccgccg
     1981 ccgcgaagga cagatcgaag tcccgttcgg gatagatggt ctgaagccaa tttttcagtt
     2041 cggtttgccg ttgcataaca gtactaaagc atttcaggtt acaataaacg ctattctaac
     2101 tggcaaaccg acttgagggg cgattttggc tcgtttattt tcactcaaac cactggtgct
     2161 ggcattgggc ttctgtttcg gcacgcattg cgccgccgcc gatgccgttg cggcggagga
     2221 aacggacaat ccgaccgccg gaggaagcgt tcggagcgtg tccgaaccca tacagcctac
     2281 cagcctgagc ctcggttcga cctgcctgtt ttgcagtaac gaaagcggca gccccgagag
     2341 aaccgaagcc gccgtccgag gcagcggcga agcatccatc cccgaagact atacgcgcat
     2401 tgttgccgac aaggtggaag gacagtcgca ggtgcaggtg cgtgccgaag gcaacgtcgt
     2461 cgtcgaacgc aaccggacga ccctcaatac cgattgggcg gattacgacc agtcgggcga
     2521 caccgttacc gcaggcgacc ggttcgccct ccaacaggac ggtacgctga ttcggggcga
     2581 aaccctgacc tacaatctcg agcagcagac cggggaagcg cacaacgtcc gtatggaaac
     2641 cgaacacggc ggacggcggc tgcaaagcgt cagccgcacc gccgaaatgt tgggcgaagg
     2701 gcattacaaa ctgacggaaa cccaattcaa cacctgttcc gccggcgatg ccggctggta
     2761 tgtcaaggcc gcttccgtcg aagccgatcg ggaaaaaggc ataggcgttg ccaaacacgc
     2821 cgccttcgtg ttcggcggcg ttcccatttt ctacacccct tgggcggact tcccgcttga
     2881 cggcaaccgc aaaagcggcc tgcttgttcc ctcactgtcc gccggttcgg acggcgtttc
     2941 cctttccgtt ccctattatt tcaaccttgc ccccaatctc gatgccacgt tcgcgcccag
     3001 cgtgatcggc gaacgcggcg cggtctttga cgggcaggta cgctacctgc ggccggatta
     3061 tgccggccag tccgacctga cctggctgcc gcacgacaag aaaagcggca ggaataaccg
     3121 ctatcaggcg aaatggcagc atcggcacga catttccgac acgcttcagg cgggtgtcga
     3181 tttcaaccaa gtctccgaca gcggctacta ccgcgacttt tacggcaaca aagaaatcgc
     3241 cggcaacgtc aacctcaacc gccgtgtatg gctggattat ggcggcaggg cggcgggcgg
     3301 cagcctgaat gccggccttt cggttctgaa ataccagacg ctggcaaacc aaagcggcta
     3361 taaagacaaa ccgtatgccc tgatgccgcg cctttccgcc gattggcgca aaaacaccgg
     3421 cagggcgcaa atcggcgtgt ccgcacaatt tacccgcttc agccacgaca gccgccaaga
     3481 cggcagccgc ctcgtcgtct atcccgacat caaatgggat ttcagcaaca gctggggtta
     3541 cgtccgtccc aaactcggac tgcacgccac ctattacagc ctcaaccgct tcggcagcca
     3601 agaagcccga cgcgtcagcc gcactctgcc cattgtcaac atcgacagcg gcgcaacttt
     3661 tgagcggaat acgcggatgt tcggcggcgg agtcctgcaa accctcgagc cgcgcctgtt
     3721 ctacaactat attcctgcca aatcccaaaa cgacctgccc aatttcgatt cgtcggaaag
     3781 cagcttcggc tacgggcagc tctttcgcga aaacctctat tacggcaacg acaggattaa
     3841 caccgcaaac agcctttccg ccgccgtgca aagccgtatt ttggacggcg cgacggggga
     3901 agagcgtttc cgcgccggca tcggtcagaa attctatttc aaggatgatg cggtgatgct
     3961 tgacggcagc gtcggcaaaa aaccgcgcaa ccgttccgac tgggtggcat ttgcctccgg
     4021 cagcatcggc agccgcttca tcctcgacag cagcatccac tacaaccaaa acgacaaacg
     4081 cgccgagaac tacgccgtcg gtgcaagcta ccgtcccgca cagggcaaag tgctgaacgc
     4141 ccgctacaaa tacgggcgca acgaaaaaat ctacctgaag tccgacggtt cctattttta
     4201 cgacaaactc agccagctcg acctgtccgc acaatggccg ctgacgcgca acctgtcggc
     4261 cgtcgtccgt tacaactacg gttttgaagc caaaaaaccg atagaaatgc tggcgggtgc
     4321 ggaatacaaa agcagttgcg gctgctgggg cgcgggcgtg tacgcccaac gctacgttac
     4381 cggcgaaaac acctacaaaa acgctgtctt tttctcactt cagttgaaag acctcagcag
     4441 tgtcggcaga aaccccgcag acaggatgga tgtcgccgtt cccggctata tccccgccca
     4501 ctctctttcc gccggacgca acaaacggcc ctgaccgtcg gaaacccggc aggagcaccg
     4561 ttcccgcaca agacggcatt ccaccgacaa ccccaaaccc gccatcaaag gcaggattca
     4621 aacgataagg aaagaatgat gaaaatcaaa gccctgatga ttgccgccgc attgctggca
     4681 gcagccgatg tccacgccgc accgcaaaag gcaaaaaccg catc
//
LOCUS       DAWEWP010000143         4650 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWEWP010000143 DAWEWP010000000
VERSION     DAWEWP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.143"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            110..1051
                     /gene="miaA"
                     /locus_tag="WGN53_001703"
     CDS             110..1051
                     /gene="miaA"
                     /locus_tag="WGN53_001703"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ0140670.1"
                     /translation="MPTPKAFALLGPTACGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSHAERSFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSPTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRLYPSLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVTDPFSDGNSCTRLIEAAKRFFGA"
     gene            1055..1774
                     /locus_tag="WGN53_001704"
     CDS             1055..1774
                     /locus_tag="WGN53_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ0140671.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGAVQTFLLLKASGLT
                     LTKSTLWQLQSLLDGTPPQEMAQHDKTRLLAELKNHPPVAAFPPQGEMAHFMGLALEQ
                     AKLSALMGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1779..2267
                     /locus_tag="WGN53_001705"
     CDS             1779..2267
                     /locus_tag="WGN53_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ0140672.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALVGSIIGMFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2333..3085
                     /gene="rlmB"
                     /locus_tag="WGN53_001706"
     CDS             2333..3085
                     /gene="rlmB"
                     /locus_tag="WGN53_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ0140673.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(3147..4538)
                     /locus_tag="WGN53_001707"
     CDS             complement(3147..4538)
                     /locus_tag="WGN53_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ0140674.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLIFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
BASE COUNT         1162 a         1490 c         1126 g          872 t
ORIGIN      
        1 aaggcatacg gtaaacacgc ccccttgccg tcggcgtgct tccgcaaacc aaaatataac
       61 gtcggcacgc tttccgctat aatccgtatt tttccaaacc tgaaacgtta tgccgacccc
      121 gaaagccttt gccctgctcg ggccgaccgc ctgcggcaaa accgcgctcg ccctcaaaat
      181 tgccgaaacc ttgcctgtcg aaatcatcag cctcgattcc gcgctggttt accgcgatat
      241 ggacatcggc acggcgaaac cgtctcatgc cgagcgttcc tttgtaccgc accaccttat
      301 cgacatcatt acgcctgttc agacatacag tgccgcccgt tttgtcgaag actgcacgcg
      361 tctgattggc gaaatcacgg cgcgtgggaa atatccgctg attgtcggcg gcacgatgat
      421 gtatttccgc gccctgaccc aaggtttgaa cgatttgccc gaagccgacg cctgcctgcg
      481 tgccgacttg gacgaacaaa aacaaatgta cggcttggat ttcctctacc gcaccctgca
      541 aaaagtcgac cctgaaacag catgccgtct gaaaccgaac gacagccagc gtatcggacg
      601 cgctttggaa gtttattatt taaccggcaa accgatgagt acgcatctcg gcagcccaac
      661 ctcccatacg ctccccttcg atttgcatac cgccgccctg attcccgaaa accgcgcccg
      721 cctgcatgaa aacatcgccc tgcgtttcca cctgatgctt gaacagggct ttatcggcga
      781 agtagaaaac ctgcgccgcc tctatcccag cctgaccgcc gactcccctg ccatacgctg
      841 cgtcggctac cgtcaggcat gggaatatct ggacggaaaa accgatttcc cggcatttgt
      901 cgagaaaggc attgccgcca cgcgccaact tgccaaacgc caactgactt ggttgcgcaa
      961 aacaccttta gactgcgtta ccgacccgtt ttcagacggc aattcatgca cacgcctgat
     1021 tgaggcggca aaacggtttt tcggcgcata aactatgctg accacgccgc cgctcgcccc
     1081 caaaaccgtc gccgccctac acaggctcgg tatccgtacg ctcgaagaac tgcggcaaaa
     1141 cggcgcggtt caaaccttct tgttattgaa ggcatcaggt ttgaccctca cgaaaagcac
     1201 gctgtggcag cttcagtcgc tgctggacgg cacgccgccg caagaaatgg cgcaacacga
     1261 taaaacccgc ctgcttgccg agttgaaaaa ccatccgccc gtcgccgcct ttcccccgca
     1321 aggcgaaatg gcgcatttca tgggtttggc attggaacag gcgaaactgt ccgcattaat
     1381 gggcgaaata cccgtcgggg cagtcatcgt ttcagacggc aaaatcatcg catcggcaca
     1441 caacacctgc attgccgact gcaatgtcag ccggcacgcc gaaatcaacg ccttggcaca
     1501 ggcaggcagc gaaatgcaaa actaccgcct tgacggatgc gacatctaca tcaccctcga
     1561 accctgcgcc atgtgcgcgt ccgcactgat acaggcacga atcaggcgcg tgatctacgg
     1621 tgcggcagaa cccaaaaccg gcgcggcagg aagcatcgtc aacctgtttg ccgacaaacg
     1681 cctcaatacg cacaccgcca tacggggcgg aatcctgcaa gaagaatgcc gcgccgtatt
     1741 aagccgtttt ttccaaaaca aaagaaaagg ttgaacctat gaccgccttg ctcgtcatcc
     1801 tcgccctcgc cctgatagcc gccggtacgg cgggcatcgt ttaccccgcc ctgcccggat
     1861 tggcattgat gtttgccgga acctggctgc tcgcctactc cggcggctac caaatctacg
     1921 gcgcgggcgt tttgtggacg gtcggactca tcagccttgc cggcatactg gcggactatg
     1981 tggcaggcat atgggggaca aaatataccg gagcgggcaa gctcgccgtt cgcggcgcat
     2041 tggtcggcag tatcatcggt atgtttttct cccttcccgg actaatactc ggtcccttta
     2101 tcggcgcggc ggcaggcgaa ctgatcgatc ggcgcaatat gcttcaggca ggtaaagcgg
     2161 gcttgggtac gctgttgggg cttgtcgtcg gcacggcgtt caaaatcggc tgcgccgtat
     2221 ccatcttgtt tatcctgttg gtgaaataca tcgcctacct gttttaaccc cttcagacgg
     2281 cataccggca aattgctaca atgccgtctg aacccttttc acaaagcacc atatggcaaa
     2341 ccaaagacct atctacggct tccatgccgt caacgcccga ttgtggcaaa accccaaatc
     2401 catcgtcgaa ctctatgtcc aagaaggcaa aaacgatgta cgcacgcgcg aagtgttgga
     2461 aaaggcggca aacgaaaaca tccgcgtata ttttgccgat gccgaccgcc tcaacgccat
     2521 cagcaaaggt gcgcgccatc agggcgtggt cggatttatc gatgcctcca aaaaccacgt
     2581 ccacctcgaa gacgtattgg aaaacttaag cgaaccgccg ctgctgctga tactcgacgg
     2641 catcaccgat ccgcacaacc tcggcgcgtg cctgcgtacc gccgacgcaa tgggcgtaca
     2701 tgccgtcatc gcaccgaaag acaaaagcgc ggggctgaac gccaccgtca gcaaagtcgc
     2761 cagcggcgcg gcggaaaccg tcccctacat caccgtaacc aacctcgccc gcaccctgcg
     2821 cgagctgaaa gaatacggca tttggatcat cggcaccgat atgggcggca acgccgacct
     2881 ttaccattgc gatctgcccg acagcgcggc atgggtgatg ggcaacgaag gcgacggtat
     2941 gcgccgcctg acacgcgaac attgcgacat gctggtgtcc atacccatgt tcggcacggt
     3001 cgaaagcatg aacgtctccg tgtctgccgg aatggtatta agcgaaaccc gccggcagcg
     3061 cgtattaaaa aacgaaaaag cgtaagcctc aaaaacaatg ccgtctgaaa cagggattct
     3121 gtttcagacg gcattcgttt tcaaaatcag tgttccaagt ggtcggtatc aaacttgacc
     3181 gccgcctcgg ttttatcttc gggcaagacc aaattcagca agactgccgt aatgccgccg
     3241 gcggaaatag agttttggaa caagacaggc aggtttttaa acacttccgg ctcaaacgcg
     3301 acacccaagc ccaaaccgac cgacgttgcc gcaattaccg cttcgcgcct gcggatgccg
     3361 tggctgacca aaatccgcac gcccgcaatc gcaatcaagc cgaacatcaa aaccatcgcg
     3421 ccgcccaaca ccggactcgg aatcgtcgta aacgcgcgtc cgacaacggg gaacagaccc
     3481 aacagcacca aaatcacggc aatatatttg cccacatggc gcgaagccac gccggtcatc
     3541 tgaatcacgc cgttgttttg cgcaaacgtc gtcagcggca gcgaacccaa agccgtcgca
     3601 atcaccgaca ccaagccgtc agccaacacg ccgccgcgca ggcgtttggt gtattcctcg
     3661 ccttcaatcg gctgatcgga caccattgcc gtcgccgtca aatcgccgac cgcctcaaac
     3721 acgctcaaca ggaaaatcgc gcccgccaca ataaatgcgt gccagtcgaa agcaaaaccg
     3781 tatttaaacg gtacgggcag cgtaaccagc ggcaggtttt gcagcgcgga aaaatccacc
     3841 ttgcccaaaa acagcgcgac aatgtaaccg gcaatcagac cgaccgcgat gccgctcatc
     3901 cgcaacagcg ggtttttcat gcagttgaat atcaacacaa tcagcaacac cagcgaagcc
     3961 agccccaagt tttccatcga gccgaacgtg ccgtccgcct tcgcgccgaa gccgccgccg
     4021 aaatcggtaa taccgacgtg taccaaactc aagccaatga gcatcacgac cacgccgctg
     4081 accgtcggcg taatcacttt tttcaaatac ggcaaaagcc acgccgagaa acacaccaaa
     4141 aacgcgccga caaacgatac acccaagagc gtcgaaatca tcgcatcctt agtcaaaccg
     4201 ccctccttca tccctgcgcc cagcgcaatc atcacggtaa cgaacgaaaa attcaccgac
     4261 tggatggaca gcatccccga accgaccggc ccgaagcggt tgacctgcaa ataagtgccg
     4321 acacccgacg caaccatcgc catcgacacg agatacgccg tcatctccac cggcaattcc
     4381 agcgcgccgc ccacaatcag cgcgggcgta atcatcggca caaaaatcgc caaaagatgg
     4441 gtaaccgcgc tcaagagcgc attaccgaac ggcggcctgt cttccaaacc gtacaccaaa
     4501 tcaggcgcat ccgcaccttt gcccaactga ccgctcattg tatttcctta ttttaaaggt
     4561 tgaaaaatcc aagcgcggat tgtagcgaat ctgccgcacg gcttcaattt gcatttcaga
     4621 cggcatccgt ctggtccaca cgtttaccac
//
LOCUS       DAWEWP010000144         4574 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWEWP010000144 DAWEWP010000000
VERSION     DAWEWP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.144"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(58..1851)
                     /locus_tag="WGN53_001708"
     CDS             complement(58..1851)
                     /locus_tag="WGN53_001708"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ0140675.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIVKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2096..3205
                     /gene="proB"
                     /locus_tag="WGN53_001709"
     CDS             2096..3205
                     /gene="proB"
                     /locus_tag="WGN53_001709"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ0140676.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSNATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3218..4480
                     /locus_tag="WGN53_001710"
     CDS             3218..4480
                     /locus_tag="WGN53_001710"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ0140677.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRLDLEAAAGNIPESMTDRLLLDEKRIGAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRNRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVW
                     HIETHSTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1045 a         1244 c         1271 g         1014 t
ORIGIN      
        1 gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca
       61 aatcgtaccg ctgccttgcg ctttcggttt ggcggcacta aattccagtt tgctcaaagc
      121 ggaaaggtag gctttggcag tggcaaccaa aacgtcggta tccgcgccct gaccgttgac
      181 gacgcggttg ccgcgtgcca gacggacgct ggtttcgcct tggctttccg taccttgcgt
      241 gacggcgttg acggaataaa tctgcaaagt cgcgccgctt tgcgccacgc tttcaatcgc
      301 tttgaaaatt gcatcaaccg gaccggaacc ggttgcggaa gcgcgttttt cttcgccttt
      361 gatgctgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttggga
      421 gatgaatttg tagctctcgg cattcatgct gcccatttcg tcggatacca gtgcgtgcag
      481 gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcggg caaacgcggc
      541 gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc
      601 gttgcggccg gacaatttgc ccaagctcaa acggtttgtt gcccagccga ccgattcggc
      661 ggacataatc tcgtaagttt cgcggtgttt cagcaccccg tcctgatgga tgcccgattc
      721 atgcgaaaag gcattggcac cgacaatggc tttgttgggc tgcacgggat agcccgtaat
      781 ggtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc
      841 gaacaagtcg tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc
      901 acgttcgccc aagccgttga cagtacattc cacctgacgc gcaccgcctt ttaatgcggc
      961 aagcgaattg gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc aaacgacttt
     1021 gccgccgttg ggcgttttga caatcagttc gcggaaaaat tcttcggttt tgtacgggat
     1081 ggaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa ttaccgcgcc
     1141 gcagatttcg gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac
     1201 atcgtcggtg tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt
     1261 cggcttcatc ttcaatttgt actccatatg gatggggctg gtggcgatga aggtgtggat
     1321 gcgttttttc ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc
     1381 gcgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc
     1441 gaaatcgccc gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc
     1501 cagctggcgg gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgactgctc
     1561 gccgtcgcgc agggtggtat caaaaataat tacgcggttg gtctgtgtca tttctgttct
     1621 ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggtctgc cttgcgattc
     1681 ggcaagcgcg gtcagctttt gcagttgcgc cagaggcagc gagccgcgcg tgcgccattt
     1741 atagatggcg gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag
     1801 cgcgttcacg ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct
     1861 tgtctaaaat aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa
     1921 aacggcaata taaatttgaa aaatctcaat aagccgccat agattttatt aattttatac
     1981 attttgtcca ttttattgca gacgcggcgt gattgttgac aattgcggca gaacagggca
     2041 aattcggcgg cacaaggtgg caacgagaca ggacaaacca acaaggatgg aaacaatgaa
     2101 atacaaaaga atcgtattta aagtcggcac atcttcgatt acccattcgg acggcagtct
     2161 ctcgcgcggc aaaatccaaa ccatcacctg ccagcttgcc gcattgcatc atgcgggaca
     2221 cgagctggtc ttggtgtctt ccggcgcggt tgcggcaggg ttcggtgcgc tgggtttcaa
     2281 aaaacgtccg gtcaaaatcg ccgacaaaca ggcttccgcc gccgtcgggc aggggctgct
     2341 gatggaagaa tatacggcaa acctgtcttc agacggcatc gtgtccgcac aaatcctgct
     2401 cagccgcgcc gactttgccg acaaacgccg ctaccaaaat gccggcggcg cactttccgt
     2461 gctgctgcaa cgccgcgccg tccccatcat caatgaaaac gatacggttt cggttgagga
     2521 attgaaaatc ggcgacaacg acacattgag tgcgcaagtg gcggcgatga tacaggcaga
     2581 cctcttggtg ctgctgaccg acatagacgg tctttacacg ggcaacccga acagcaatcc
     2641 cgatgccgta cggctggaca aaatcgaaca catcaaccat gaaatcatcg aaatggcggg
     2701 cggctcgggt tcggcaaacg gcacaggcgg tatgctgacc aaaatcaaag cggcgacgat
     2761 tgcgaccgag tccggcgtac cggtgtatat ctgttcctcg ctcaaacccg atgcacttgc
     2821 cgaagctgcc gaacatcagg cggacggctc gtttttcgtc ccccgtgcca aaggtttgcg
     2881 gacgcagaag caatggctgg cgttctattc cgaaagccgg ggcagcgttt atgtggacga
     2941 aggtgcggaa cacgctttgt ccgaacaggg gaaaagcctg ctgatgtcgg gcattgccgg
     3001 aatcgaaggg catttttccc gtatggacac tgtaaccgtg tacagcaacg caaccaaaca
     3061 gcctttgggc aaagggcgcg tcctgttcgg ttctgccgcc gccgaagacc tgctcaaatc
     3121 gcgtaaggcg aaaggcgtgt tcatccatcg ggacgactgg atttccatca cgcccgaaat
     3181 acgcctgctt ctgaccgaat tttagaaaag gaaacccatg tcaaacacac aaaaacagct
     3241 tgcccttgcc aaagcggcaa aaaaatccgt caacacggcg gatacggaag aaaaaaaccg
     3301 cgcgctgctt gctatggcgg acagcttgga agcggcggcg gcagatattt tggcggcaaa
     3361 ccgtttggat ttggaagcgg cggcaggcaa cattcccgaa agcatgaccg accgcctttt
     3421 gttggacgaa aaacgcattg gcgcgatggc ggacggcatc agggcggttg ccgcgctgcc
     3481 cgaccccgtg ggcgaaatac tggaaacctc gactctgccc aacggcttgg aaatcgtcaa
     3541 aaagcgtgtg gcgatgggcg ttatcggcat tatttacgaa agccgcccga acgttacgtc
     3601 cgatgcggcg gctttggcac tgaaaagcgg cagcgcggtc gtactccgca gcggcaaaga
     3661 tgcattccaa tccgcacgcg ccatcgttgc cgccctgaaa acggggttgg cacaaacccg
     3721 catcgacccc gacgcgctcc agttgattga agacaccggg cgcgaaagca gttacgaaat
     3781 gatgagggcg aaagattatc tagacctgct gattccgcgc ggcggggcgg ggctgatacg
     3841 ggcggtggtt gaaaatgccg tcgtgccggt cattgaaacg ggaacgggca tcgtccacat
     3901 ttatatcgac aaggacgcgg attgggacaa ggcgctccgt atcgtgtaca acgccaaaac
     3961 cagccgcccg tccgtgtgca actcgatgga agtgctgctg gtgcatgaag acattgctgc
     4021 cgacttcctg cccaagctcg aacggctgtt ggttcgcaac cgtatagaag ccggactgcc
     4081 gcccgtccgc ttccgtttgg atccgcaggc ggcgcggcat atcggcggcg aagcggcggg
     4141 tgcagacgat ttcgataccg agtttttaga ctacatcctc gctgtgaaaa ccgtcgcttc
     4201 ggtcgaagag gcggtctggc acatcgaaac ccacagcaca caccattcag acggcatcgt
     4261 taccgaaaac cgccacgctg cggactattt cacgacccat atcgattctg ccgccgtgta
     4321 tgtcaacgcg tccacgcgct ttaccgacgg cggcgaattc ggcttgggtt gcgaaatggg
     4381 catctccacg caaaaactcc acgcccgggg tccgatgggt ttgaaagagc tgacgagtta
     4441 caaatacatc gtacaaggca cgggacaggt cagggaataa tccgaattca aatgtctgat
     4501 atagtggatt aactttaaac cggtacggcg ttgcctcgcc ttagctcaaa gagaacgatt
     4561 ctctaaggtg ctga
//
LOCUS       DAWEWP010000145         4554 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWEWP010000145 DAWEWP010000000
VERSION     DAWEWP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.145"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(104..1042)
                     /locus_tag="WGN53_001711"
     CDS             complement(104..1042)
                     /locus_tag="WGN53_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0140678.1"
                     /translation="MLELNGLCKRFGNKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIWLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1051..1925)
                     /locus_tag="WGN53_001712"
                     /pseudo
     CDS             complement(1051..1925)
                     /locus_tag="WGN53_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
     gene            complement(2107..2183)
                     /locus_tag="WGN53_001713"
     tRNA            complement(2107..2183)
                     /locus_tag="WGN53_001713"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2147..2149),aa:Met,seq:cat)
     regulatory      2333..2432
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2523..4424
                     /gene="thiC"
                     /locus_tag="WGN53_001714"
     CDS             2523..4424
                     /gene="thiC"
                     /locus_tag="WGN53_001714"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ0140679.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGGNVTQLHYARRGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTADRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTSKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1111 a         1304 c         1199 g          940 t
ORIGIN      
        1 gttgtttcgg ataaattttg cagccctgat aaaaaatatg gctgctttgg taaaaaaatg
       61 ccgtctgaaa ggttttcaga cggcattttg tttttaagaa gcatcaacgg aagcggacga
      121 tttccctgtc ttcgatatgg atgcgtaccg tatcgtttcc cgatatttca ccggcgtgcg
      181 gcatatcgag gttcagccac aggatgccgt gttccggatg gaggacggac aggctgaacg
      241 attcgggcaa acaggtacgg gataatacgc ggcactccat gccgtcttgg tcgaaacgca
      301 ccgcatgttg cggaatatgg cggtcatcgt cggtattggg caaacccatc agtcgggcga
      361 cctgcacgcc ggcaggcgtt tgaaccaagg tttcgggcgt accgcattga aggattttcc
      421 cctcgtgcat gacggcgatt tcgtccgccg ccgtgcaggc ctcttcgggc gaatgcgtca
      481 ccaaaacggc agggatgccg cccttgcgga tacgttcggc agtcatgcgg cgcagccggt
      541 cgcgcaaatg cgtgtccaaa ctggaaaacg attcgtccaa cagcagcagg gaagggcgga
      601 caaccaaagc gcgcgccaac gccaaccgtt gcttctcgcc tccggaaagt ttttcaggct
      661 tgcggtgcgc ctcgttttcc agtccgactt cggcaagtgc cgccatggca aggcgttcgg
      721 cttcggcttt cggcattttt tgcattttca aaccgaatgc cgcattttcc agcgcactca
      781 tatggggaaa cagcgcgtaa tcttgaaaca tcagagaaat acggcgtttt tcgggcggca
      841 tacgggtaat gttttccccg ttcagccata tttccccacc gtccggccgg acaatccccg
      901 caatcatatt cagcagggtg gattttccgc atcccgaccg ccccaaaacg gcgagtattt
      961 tgccgcgccc gacagtcagg cagatgttgt cggcgacggt tttattgccg aagcgtttgc
     1021 agagtccgtt cagttcaagc atggcgcatc ctataaacgt atgccgtcca gccactcgga
     1081 cagcggatgg gcggcatcaa tcagaccgta acggcaaaac gcgtccagcg taaccagttg
     1141 cgcgtcgtgc atcatgtttc ccgacaacat ggtggcaacc aagctgccga tgtccatttt
     1201 ctcaaaacac gcaacctcac cgtcctgatt ttcaggtagg aaggtttcgg gcaggacggc
     1261 atcgaataca tacaggattt cattgtgcac accccagctg acggggcgca ggctgtgcag
     1321 ctgcgatacc gggcggatga gcggaaacag cgttttatcc aaaccggctt cttcgctgct
     1381 ttcgcgacac acggtttcag acggcaattc accgctggaa acaccgccgg cggcagtatt
     1441 gtcgagtttg tcgggatcga ctgctttgtg cggactgcgc ctgcctatcc agaaatgcca
     1501 tcggccgtcc gattcggtca gaccgttgag atggacggcg cggctgagta gtccgaacgg
     1561 gcggaaagcg gcgcgttcaa gtgagaacaa gatattgccg ccgccgtcgg tcggtcgaaa
     1621 cactcgttgc gccatccgtg aagcagcccc gccttgttcc atgtgcgggt gaggtgctgc
     1681 aagcggccgc ccatatctga ccagccgtcc gcattcagaa aaatgccgtc tgaagactcc
     1741 gagcagcctg cctcccagtc ttttttgacg cgctccgccc attccggcga caggttgccc
     1801 aaaggcaggc cgttcagata cagtgttttc cagcaacttt ctgcaccgta acttgctttt
     1861 gcccactcga acagggcatc aaggtcgtgt ttgctgacgg attcggtaaa acggacggtc
     1921 ggcatagtcg gctttcttga agacaatgcg gaagattgta gccaaactgc ctgaatttgc
     1981 aaccgcaaac agacggcggg ctgccgttcc gatattgcgg caggaaggaa aatccggcaa
     2041 aaaacggagg cggcaggaaa caggcaaaac aaaacccctc ggcattgaac cgaggggttt
     2101 gatatttggt tgcgggagca ggattcgaac ctacgacctt cgggttatga gcccgacgag
     2161 ctaccatgct gctccatccc gcgtcagaag atgaaactat acggcagatt ttttatgttg
     2221 tcaaacatta tttccgcgaa aaatcataaa attttgccgt ccggcggttt atgcctgatt
     2281 tgaaatatca ttttctttac aaaagttcat gttcgtgatt taattttggt taacatcgaa
     2341 acaggggtgc tgcctgatgt tcaggcggct gagaaatacc ctttacaccc gatcgggata
     2401 atacctgcgt ggggagtttt cacggattct gtctttcaga cagcattggt tttcaaatgc
     2461 cgtctgaaaa cgcaaaacgc tcctgtttct ttattctaaa cgagaaaaca ggagcatttt
     2521 ttatgactac gccaaaaaaa accgccaaaa cttccggcaa cgaagcgcgc gaacttgccg
     2581 acttgagcga agacatcggt atccgcttca aatatccgaa ctcggaacgc gtgtatctgc
     2641 aaggcagccg cgacgacatc cgcgtgcctt tgcgtgaaat ccgtcaggac gacacctata
     2701 cggcgcaagg cgcggaagcc aacccgccga ttcccgtcta tgacaccagc ggcgtgtacg
     2761 gcgatccagc ggcacacatc gacctgaaac aaggcctgcc gcacatccgc accgcatggc
     2821 tgaacgaacg cggcgatacc gaaatcctgc ccaagctctc cagcgaatac ggcatcgaac
     2881 gcgcacacga tccgaaaacc gcccatctgc gcttcaacca aatcacccgc ccgcgccgcg
     2941 cgaaaagcgg cggcaacgtt acccagcttc actacgcgcg ccgcggcatc atcacgcccg
     3001 aaatggagtt tgtcgccata cgcgaacgtt taaaattaga cgaattgtcc caaaaatcgg
     3061 aatacgccaa actcttgaaa cagcacgcag ggcaaagttt cggcgcgaat atcccgaccc
     3121 atcccgacca aatcacgcct gaattcgtgc gccgagaaat tgccgccgga cgcgcgatta
     3181 tccccgccaa catcaaccac cccgaactcg aaccgatgat tatcggccgc aacttccgcg
     3241 tcaaaatcaa cggcaacttg ggcaattccg ccgtcacttc cagcctgacc gaagaagtcg
     3301 aaaaaatggt gtggtcgctg cgttggggcg cggacacgat tatggatttg tccaccggcg
     3361 cgcacatcca tgaaacgcgc gaatggatta tccgcaacgc gcccgtcccc atcggcaccg
     3421 tgccgattta ccaagcgttg gaaaaaaccg gcggcattgc cgaagatttg acttgggatt
     3481 tgttccgcga caccttaatc gagcaggcgg aacaaggcgt ggactatttc accatacacg
     3541 caggcgtgtt gctgcgttat gtgccgatga ctgccgaccg cctcaccggc atcgtatcgc
     3601 gcggcggttc gattatggcg aaatggtgtt tggcacatca tcgggaaaac ttcctttaca
     3661 cgcatttcga cgaaatctgc gaaattatga aggcgtatga cgtatcgttc agcctcggcg
     3721 acggtttgcg tcccggctgc attgccgatg ccaacgacga atcccaattc gccgagctgc
     3781 acaccttggg cgaattgacc tccaaagcgt ggaaacacga cgtacaggtg atgatcgaag
     3841 gtcccggcca tgtgccgctg caacgcgtca aagaaaatat gaccgaagag ttgcaacact
     3901 gttttgaagc accgttttac accctcggcc cgctcgttac cgacatcgcc cccggctacg
     3961 accacatcac ctcgggcata ggcgctgcca atatcggctg gtacggcacg gcgatgctct
     4021 gttacgttac ccccaaagaa catttgggac tgcccgacaa agaagacgtg cgtaccggca
     4081 tcatcaccta caaactcgcc gcccacgccg ccgacctcgc caaaggctgg ccgggcgcac
     4141 agttgcgcga caacgccctg agcaaggcgc gtttcgagtt ccgctggcgc gaccagttcc
     4201 gcctaagcct cgaccccgaa cgcgccgaaa gcttccacga cgaaaccctg cccgccgaag
     4261 gcgcgaaaat cgcccacttc tgttcgatgt gcggccccaa attctgctcg atgaaaatca
     4321 cgcaggaagt gcgcgactac gccgacaagc aaaaagccca acggcagggc atggaggaaa
     4381 aagcggtcga gttcgtcaaa aaaggagcga agatttacag ctaaaacgtc aagcaaaaaa
     4441 tgccgtctga aacccggaaa aaaggcttca gacggtattc tttcgcttgt gaaaatatag
     4501 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat agta
//
LOCUS       DAWEWP010000146         4523 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWEWP010000146 DAWEWP010000000
VERSION     DAWEWP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.146"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1017..1787)
                     /gene="xth"
                     /locus_tag="WGN53_001715"
     CDS             complement(1017..1787)
                     /gene="xth"
                     /locus_tag="WGN53_001715"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0140680.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMARHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSTVERQWFQNLLDLGLTDSLRHIHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRVDLETRALERPSDHAPVAAEFDW"
     gene            complement(1847..2128)
                     /locus_tag="WGN53_001716"
     CDS             complement(1847..2128)
                     /locus_tag="WGN53_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0140681.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLSRLRVEGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAV"
     gene            2185..2463
                     /locus_tag="WGN53_001717"
     CDS             2185..2463
                     /locus_tag="WGN53_001717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140682.1"
                     /translation="MIVIIFINLIINQIFMDDCTGLYVPFPTFFANTRKGGLSDGIKL
                     PPLPCLTLLPNGIIRIVQKMPYNRRTSCPADASVPSGKQNTCLKCCFF"
     gene            complement(2596..3477)
                     /gene="nadC"
                     /locus_tag="WGN53_001718"
     CDS             complement(2596..3477)
                     /gene="nadC"
                     /locus_tag="WGN53_001718"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ0140683.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGISTATARAVAEVAKYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCDSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3552..4490)
                     /locus_tag="WGN53_001719"
     CDS             complement(3552..4490)
                     /locus_tag="WGN53_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140684.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVGRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIEEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT         1040 a         1134 c         1205 g         1144 t
ORIGIN      
        1 ctgatgcaga tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
       61 caaatagtac ggcaacgctg tactggttta aatttaatcc actatatttt gtaatagctg
      121 ccattatgcc tactaggata tataccttct atataatcct tatttaaata aaaaccctta
      181 taaaaatctg tgttatctgt cgggattgaa aaaaagattt tatctttctc atcaggttta
      241 tatttgctat caatatcaaa aagagaataa ggattacaat gagttcgaca tactaggtaa
      301 aaatttatat cttggatcat gaaatattcg atatttatca ggatcgaaag atgctaaaaa
      361 ttcatcaata tcggtactgg atattttgtc atcactaata aacatcgcta gagatgggcg
      421 actttgattt tccatttgtt gattagtaaa ggatataaat actaacttgc cattgtcaaa
      481 ccacatagat ttatatagtg gattaaattt aaaccagtac gtcgttgcct cgccttgccg
      541 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatag
      601 ttattgcctt tgggagaatc aattctgaca gcacataaca taggttcatc tttacagtcg
      661 ttatattggg tttcatcaaa ctcaactgca tagcacaata aaggcaatag gaaaaaaagg
      721 agattaattt attactatac ataaggcagc ctgattattc attctaaaat aaatccggtt
      781 tttaatattt gattctgctc cggattatat acataataaa ttttactgct ccaaaatctg
      841 atattaaccc tatggatttc ataaattttt cagaaaaata ggctgtgctt gaacaatccg
      901 ccgactccgg ctgtttgtca gggaaaaggc aattattttc ggttagtcag cagcctatcc
      961 tattgaatca cttaaagata tggatgtttg tcaacgccat atcaaaacac ggtcttttac
     1021 caatcgaatt ctgccgccac gggcgcgtgg tcgctcggac gttccagcgc gcgcgtctcc
     1081 aaatcgacac ggacatcctt caaaacggcc gccatttcgg atgtaaccaa tatatggtcg
     1141 atacgcaggc ccagtttgcg ctggaacatc gcgccgcgat agtcgaacca tgtgtaaaac
     1201 gcaccttcgg gatggatatg gcgcaaactg tcggtcaatc ccaaatccag caggttttga
     1261 aaccactgcc gttcgacggt cgaacagtgg attttttcat accatttttc agggtcgtaa
     1321 cagtccgcat cggcaggcgc gatattgaaa tcgcccaaca gcaccagttt gccgtggcgt
     1381 gccatttcat cgcggacaaa ttcggttaac gcggcaaacc attgttcttt atatttgaat
     1441 ttcgggctgt cgagggcttc gccgttgacg caatagacat tgatgacgcg cacgccgccg
     1501 acggttgccg caatcacgcg ccgttgcgga tcgtccggca gcgcgggcag cccgatatgc
     1561 acgtcttccg gcgcgttgcg gctgacgatt gccacgccgt tgtaggtttt ctgcccgctc
     1621 caaacacagt gccagcccat catctgcaag gcggcagccg gaaatttgtc ctgatcgagt
     1681 ttgagttcct gcaaaaccaa aatatcgggc ggattgtcgg caagcaggtt ttgcacctgc
     1741 ggcagccgca cattgagcga attgacgttc caagtggtga ttttcataac atttccgaaa
     1801 aatgccgtct gaaacgttca gacggcaaaa ggaaaggatt ttaacactat accgcgcgtt
     1861 tgttttccaa atcgcggatt gtccgcagaa tcgttgccgc gtcttcggaa atcaggcggt
     1921 attcgataat gcggtggtaa cgcgtaaagt ccaccagacc ttccacgcgc agtcggctca
     1981 aatggccgga aacggcggtg gccggcaggg ataaggattt tgccagttcg gcgatattgc
     2041 gttcgctgtc caacagttgg atcagtatcg ccatacgttc gggatgcgcc ataagtttga
     2101 gtatggtgtg gagcggtatt gtgttcatcg gtattcctaa aacctttgcc gcccgccaag
     2161 cggattgacg ggcgacagat atggatgatt gtgattatct ttataaatct tattataaat
     2221 caaatattta tggatgattg tacaggattg tatgttcctt ttcccacttt ttttgcaaac
     2281 acgcgtaaag gcgggctttc agacggcata aaactgccgc ccctaccctg cctcaccctg
     2341 ttgccgaacg gaatcatccg cattgttcaa aagatgcctt acaaccgccg cacgtcttgc
     2401 cctgccgatg cctccgtgcc ttcgggcaaa caaaatacct gcctgaaatg ctgttttttc
     2461 taaagcatca cgaccttcac gaaaaatgcc gtctgaagaa tctttcagac ggcatttcct
     2521 ccttgaggca ataaccggat tacggttagg cggttccttg tctgacatat cagacaactg
     2581 ccgacaccct aaaactcatg ccaagaaatc caaacctatg tccaacgagc ggctgctgtg
     2641 ggtcagatag ccgagggcga tgccgtccac gccggtttgc gccacgcgct tcaggcggtc
     2701 gaagccgatg ccgcccgatg cttcgcaata gacggtgtgg gggtgggcgg tttgcgtgtg
     2761 gcagcggttt gccgcttctt tcagggtttc gtcgtccatg ttgtccagca aaatccgttc
     2821 cgcgcccgct gcgatggctt cgtccagttg tgccaacgtg tccacttcga tttctacgca
     2881 ggtcaacggt ccgaccgcct gttttgcctg ccgaacggct tgggcgatgc tgtcgcaata
     2941 ggcgaggtgg ttgtctttga tgagtacggc atcgtccaaa cccatgcggt ggttcacgcc
     3001 gccgcccgcc ctgacggcgt atttttgcag gacgcgcagc agggggatgg ttttgcggct
     3061 gcacacgatg tctgtaccgt atttggcgac ttcggcaacg gcacgcgcgg tggcggtgga
     3121 gatgccgctc aagtgcgtga ggtagttgag cgcggtgcgt tcggcggcga gcagcgcgcg
     3181 ggcgttgcct tcgacggcgg caagcgtctg acctgcgcgg acgacttgcc cgtctcggat
     3241 ttcggcttgg aagcggacgg acggatccat cgtctgaaag gcgaggcgcg ccaagtccat
     3301 gccggcgata acaccgtctt cgcggctgac gaggaaaagt ttggcggttt tatcgggcgc
     3361 gatgacggcg gcggacgtaa tgtcgccgcg cctgcccaaa tcttcgctca aggcttgttc
     3421 caccatgggg cgcaacaggg tgtcgggcag tgggaagagg gtgttttcag acggcatggg
     3481 gtttcctttg ttgaaaaaga ttgatgttgc accgatgggc aaaacttcgt ttgatgtcgt
     3541 ctgaaaacgg gctaacggct gcccagcggc aatccgatgt ccgaaatcag cctgtcgggc
     3601 aggacgtcgt cgcggaagcg gcaaagctgc gcgggacggc ctttgctgcc cgatacgccg
     3661 gtatccgacg gctcgataag gttttgctgc tgaatctggc ggcggaagtt ttgcttgtgc
     3721 agcaatctgc cgctgatggc ttcgacgctg ttttgcagtt gcagcaacgt gaattcgggc
     3781 ggcatcagtt cgaaaatcac ggggcggtat ttgattttgg cgcgcaggcg ggacagggcg
     3841 gtcgccagta cgcggcggtg gtcgtggcgc atgggctgcc ccgtaagcgc gaagtcgaag
     3901 tttgcctgcg gctcggcggc ttcctctatc aggccgcttt catacaacat ttcatagcgt
     3961 tgcaaaacgt attcttccga ccagttttcc ggttcgacgc cccaacacaa atgaatgcgc
     4021 ttgagccgct tttggcggac ttcctccgtg tccgccgagt ttgcccaaat gcgcaggcgg
     4081 ccgacgacgg cgtcgcgctg cccgccgtcg gtgcgcaagt cttcccacgg gaaatagccg
     4141 tagcagtcct gccatttcgc atcgggatgc aggatgctgt cggctgcctc gcgcaccagc
     4201 cccaaatagc tgacgtacaa gacgggcatg ccgtgttcgt tgcggcggtg ggtatcgaca
     4261 aaggtgtaaa gctgttccac atagcccata ggctgcgaag tctgcttggc aacccacagc
     4321 ttcacgcccg cctgcaggga attgcgcaga ggggagagcg ggccgttggg caggagcgtg
     4381 ccttgggcga cggtcaacac ccgcaggccg ccgtcggtaa cggcaatcaa tacgggaacc
     4441 agctcgacga tgctttgcgg cggggcttcg gcttcggggt aggcatccat gctcggcttt
     4501 ctgcgggtgg aaaaggcttt att
//
LOCUS       DAWEWP010000147         4510 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWEWP010000147 DAWEWP010000000
VERSION     DAWEWP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.147"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(127..1269)
                     /locus_tag="WGN53_001720"
     CDS             complement(127..1269)
                     /locus_tag="WGN53_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ0140685.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGVGGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WQVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1318..1740)
                     /locus_tag="WGN53_001721"
     CDS             complement(1318..1740)
                     /locus_tag="WGN53_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140686.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1733..2542)
                     /locus_tag="WGN53_001722"
     CDS             complement(1733..2542)
                     /locus_tag="WGN53_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0140687.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCNDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2624..3001)
                     /locus_tag="WGN53_001723"
     CDS             complement(2624..3001)
                     /locus_tag="WGN53_001723"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ0140688.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3201..3968)
                     /locus_tag="WGN53_001724"
     CDS             complement(3201..3968)
                     /locus_tag="WGN53_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0140689.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSIFGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3978..>4510)
                     /locus_tag="WGN53_001725"
     CDS             complement(3978..>4510)
                     /locus_tag="WGN53_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ0140690.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKAPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVKIIKNASDIQEFSAQDDGLIIIDNVNGLHAGT
                     DYTDKNRHYIRARITV"
BASE COUNT         1106 a         1130 c         1096 g         1178 t
ORIGIN      
        1 cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
       61 aatatcggcc ggatattaaa aaaagccgtc tgaacatttt tcagacggct tttttgtaca
      121 ctttcctcaa cggttcgatc cctgcaccat atcaatcagg aacaggcggc agtcgaggtt
      181 gccgttataa agcggaattt tccgcttggg cgacaggcgc atgaatttgg gcatttccct
      241 gtcgccggta aacataccga cctgccagcc ggcatagtgt tttttcaacc acgtccccaa
      301 ctgcggatac agtgcctgca aagcgcggac ttcctcaagg cgcacaccgt agggcggatt
      361 ggacaccata atgccgccca cgccgttcgg acggacggat tgcgcgtcgg caacgctgaa
      421 ggaaacgatg tcgtccaccc cggcactgcg tgcgttggcc aatgccgtct gaatgatgcg
      481 gcggtcgctg tcgctgcccg aaatcggtgc aaaagccggt ttaacctgcg cttcggcacg
      541 gcggcgcaaa tccgaccaca gcgttttatc gaaattttgc agtttttcaa aaccgaaacg
      601 gcgcatcata cccggcgcgc ggcgggcggc aatccaagcg gcttcgatag caatcgtgcc
      661 gctgccgcaa aacgggtctt gaaacggctg cgtgccgtcg tagcctgccg agagcagcaa
      721 tccggcggca aggttttcgc gcagcggggc ttcgccggta tcgagacggt agccgcgttt
      781 gaacagggct tcgcccgaag tgtcgatgaa gatttcgaca ttgcgttcgt ccaaaaaggc
      841 gtggatgcga acatcgggcg cggctttgtc cacgctcgga cgtgcgtcgt aaatgtcgcg
      901 gaaagcgtcg cagacggcat ctttgacggt cagtccgaca aattggaggc ttttgacgtt
      961 ggcacgcttt gcctcgattt tgactttgaa cgtctgctgc aaggtaaacc agttgaacca
     1021 gttgatgttt ttggcaagtt tgtaaatgtc gcgctcggtt cggtatgtgc ctttggtcag
     1081 gcgtagcagg atgcggccgg cggtacgcga gtgcaggttg gcggcgtaaa cctgttccaa
     1141 tccgccccgg caggaaacgc cgccgtcaaa cacttgtaca tcggtacagc cgaggctttc
     1201 gagttcttga gataaaacgg tctccaagcc gcgcgggcag gtggcgaaaa gtgtatataa
     1261 agtattcata acgtgtcctt tccggacgga tgttccggca atggcggatt gaaaggttta
     1321 ttcggcggta aatgccgtct gaaatgtccg actgccgatt gtaatgtccg ccgtaaaatc
     1381 gcgcctgcct tcgacacaga tgggcaggcg gatgcgtact gcctgccaag tccccgacgg
     1441 ttgccgctcg aacatatagc ggttgaaacc catatccata tttttcatac tgaagctgat
     1501 gctgacctgt tccgtgccgg cgggcgcgtg ttcgatataa atatcaaacg gttttttggt
     1561 tgaaacggcg gcggcgcgga cgcggcttcc gtccggcagc gtgcaaccct cggtcaaatc
     1621 gcattgcgcc gccacagctt gcggctgctg cgcctgccac cattgcaaca aaacgagctt
     1681 gacggcggca aaggcaatca gcagcagtgc ggcaagcagt aatttacggt ttttattcat
     1741 gattcttccc tattgtttca tcccttacca ccacgtgcgc ccccaatccg agccatagtt
     1801 ttccataacg tgcaaccgtc tgtccgtttg ctgcaagtac gagcggcacg gatacgccct
     1861 gccgcagggc ttctgccgcc tcttgtgcct gaacatcatt gcacacgcgc cacacgacaa
     1921 catccgcgcc cttttcctgc gcctgccgcc attgttcgcg ggtctgcacc atcatccaca
     1981 cgctgtcggc gtgttcgggt ctgtcctgcc attgcgccgc ctccatcaaa acgttcgcac
     2041 cgcttccctc tgccgaaccc aaaggcagga cgcggtacgc gcccatactg ttctccccgt
     2101 gcaaacccgt tttcaggctg ccgtacaaac ggcgcggaac ggacagcgaa cgcaaaagcg
     2161 cgccgttggc gggcagcatg ggggcaacgg taaaatcacc cgccttctgc caagaccatt
     2221 cctgcccttc gcgggattgc ggttcgcccg tccattggcc ggggttgacc cataggaatt
     2281 tcaggcagac gcgggcgtgt tcgtaggaat ggattttggt cagccaaggc gtggcggcaa
     2341 ggatgcggat gccgagttct tcttcaaact cgcgttgcaa ggcttggaag tcggtttcgc
     2401 ccgcttcgac cttgccgccg gcaaattccc aatatccggc atagggtttg ccttcggggc
     2461 gcgagctgag caggtagttg ccgtctgaat cgagcaggat gccggcaacg acgcggataa
     2521 ggggtcgggt gtcttgaatc atggcgggta cgcggttggt cggtcggagt gcaaatgagg
     2581 gacgagcaat gccgtcaacg ggtttgccgc atttcagacg gcattatttt tcggcaacga
     2641 caaacgccaa tacggtgtct tcttcgtcgc tcatgctgag gctgacgcgg ctgatgcctt
     2701 gttcctccag ccatttggac agggcggggc cgtagaaaaa ttcgggcttg cccaatgcgt
     2761 catgcccgat gccgatgttg cggaaggaaa ccgcgccgcg tatgcccgtg ccgacggctt
     2821 tggcgaaggc ttctttggcg gcaaagcgtt tggcgaggta gttgacgggt ttgcccgctt
     2881 gcggaaattc aagcagctct tccggagtga ggatgcgccc ggcaaacgcc tgtccgaatt
     2941 ttttgtttaa gcggatgatg cgcttgaggg aaacaatgtc tgtgccgatg ccgtaaatca
     3001 tatttgcgct ccttcgccct tggttgcggg tagtgatgaa tgtgatgatg aatgccgtca
     3061 atttcttggg gcgttaaaat ggcgtgaata tccacttttg gggttttgtc ggtaacaatt
     3121 ttcactgtaa ccttttgcat ttgaaattct aaaacatcgt gccaattatt taataaggag
     3181 ttaaggcatt taataatgaa ttaaagtttg attaaacccg aatgcccgat tttcagagcc
     3241 aataccaata aaacaataaa aattaacatt ttaaaaaaat ttgggctaat cttagtcctt
     3301 aaccgaattc caacatacaa tccaataaca gacaatacgg acagtaaaaa tattaaaccg
     3361 tattcactct tatttaataa ccaatactgg tctcttagca tatatatttg aacaattttc
     3421 gccaaaagat agcatagatt gcttgatttt acgatacgat ttttattttc tgtttcgcta
     3481 agcaaaaata ttaacaatat gggagacatg gcattggttg aaccgccgat gatgcctgcc
     3541 aaaaacccaa aaagaaccat attcttatta ttggcaacta cttgaatatt ttttgctttt
     3601 gcacatacat ttaaaatacc attgacagaa taatacaatg taatgattgc catcagtaaa
     3661 agcagccaag acactggaag tatcaaaagc aacttcaccc caaaaatgct gccaacgacg
     3721 ctgccgatag caagcaattt ataggttttt aaataataaa caatctcttg ccaaaaacct
     3781 tttttgttat tgctgcatag aaccaacaag ctcattaaca ggcttggtaa tgccaccaag
     3841 gcaacaacct tagacaatgg catgttaaaa gccaatgcgg ttgtaccgag catcggaaat
     3901 cccatgcctg taattccgtg cagtattgcg gcagcaacaa aaacgataga ttgcattatt
     3961 tcttgcataa ccgtccttta tacagtaatt cttgctctaa tataatgcct gtttttatcc
     4021 gtataatcag ttccggcatg caagccattg acattgtcaa taataatcaa accatcatct
     4081 tgtgcagaaa attcttgaat atcactcgca ttttttataa tctttacaag ataatcaagt
     4141 gcaagtacca tttcctttga tactttattt ctattttctt caataccttt atagatacaa
     4201 tcacttctaa atctaatcat ttgagtattg accgataaga tattacccca tctcacacct
     4261 tgttttttat caaatgatgc tggtgcttta aatggatata aattgcccgt taatgttttt
     4321 aagtgttttt taccggtttc tgttttagat aactgattga caatatctga tgaacttaaa
     4381 aatagggaat tacctccatc attggctgat tttactacat acataaccaa ataactttcc
     4441 gggttttcac taaaagatga atcactatga agtggacatt ctccaacatc ttccgagaaa
     4501 gttatatcat
//
LOCUS       DAWEWP010000148         4387 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWEWP010000148 DAWEWP010000000
VERSION     DAWEWP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.148"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            281..1402
                     /gene="dnaJ"
                     /locus_tag="WGN53_001726"
     CDS             281..1402
                     /gene="dnaJ"
                     /locus_tag="WGN53_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ0140691.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1598..3616
                     /locus_tag="WGN53_001727"
     CDS             1598..3616
                     /locus_tag="WGN53_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ0140692.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMIIFARRVLKAGR"
     gene            complement(3660..4217)
                     /gene="rfbC"
                     /locus_tag="WGN53_001728"
     CDS             complement(3660..4217)
                     /gene="rfbC"
                     /locus_tag="WGN53_001728"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0140693.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMRKDSPTFGKWVGEILS
                     AENKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPEAEHSLIWNDPTVGIDWPLQGE
                     PNLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(4271..>4387)
                     /locus_tag="WGN53_001729"
     CDS             complement(4271..>4387)
                     /locus_tag="WGN53_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEZ0140694.1"
                     /translation="LEEIAWRNGWLSDEKLEELARPMAKNQYGQYLLRLLKK"
BASE COUNT          922 a          991 c         1295 g         1179 t
ORIGIN      
        1 gataaaaaat aaaataaggg gaagtctgca gccgcatcaa atgccgtctg aacatgcgtt
       61 cgggcggcgt ttttataaca aaaacacttc atggcggttg gttttatgcc tgtctaagtt
      121 tttgtgtcgt gcatacctga agatttcaga cggcatcggt ttatgctgtc tgaaaagtgt
      181 attccgtttc agtttgtaag ctatggcagt ctgtttgtct tgtgttttgc gcaattgccc
      241 ttattttgag ccgtgatttt attttgaatt agatgaaaaa atgagtaatc aagattttta
      301 tgcgacgctg ggtgtggcaa gaacagctac cgatgatgag attaaaaaag cctaccggaa
      361 attggcaatg aaataccatc ccgaccgcaa tcctgacaat aaagaggcgg aagagaagtt
      421 taaagaagta caaaaggcgt atgaaacttt gtccgacaag gaaaagcgcg ctatgtacga
      481 ccagtatggt catgcggcgt ttgaaggcgg cggacagggg ggcttcggag ggtttggcgg
      541 atttggcggt gcgcagggtt ttgactttgg ggatattttc agccaaatgt ttggaggcgg
      601 ttcggggcgc gcccagcctg attatcaggg tgaggacgtt caagtcggta tcgaaatcac
      661 gcttgaagaa gccgcaaaag gtgtgaagaa acgcatcaat attccgactt atgaagcgtg
      721 tgatgtctgt aacggcagtg gcgcgaaacc ggggacatcc ccggaaacct gcccgacttg
      781 caaaggttcg ggtacggtgc acatccagca ggcgattttc cgtatgcagc agacttgtcc
      841 gacctgccac ggtgcgggca aacacattaa agaaccttgc gtcaaatgcc gtggcgcggg
      901 gcggaataag gcggtcaaga cggtggaagt caatattccc gccggtatcg atgacgggca
      961 gcgtatccgt ttgagcggcg aaggcgggcc gggtatgcac ggtgcgcctg ccggcgactt
     1021 gtatgtaacc gtccgcattc gggcgcataa gattttccaa cgcgacggtc tggacttgca
     1081 ttgcgaactg ccgatcagtt ttgccacggc tgctttgggc ggggagttgg aagtgccgac
     1141 cttggacgga aaggtcaagc tcaccgtccc caaagaaacc caaaccggca ggaggatgcg
     1201 cgtgaagggt aagggtgtca aatctttacg cagcagcgcg accggcgatt tgtactgcca
     1261 tattgttgtc gaaacgcctg tcaatttgac cgaccgtcaa aaagagcttt tggaagaatt
     1321 tgagcggatt tctaccggct tggaaaacca aacaccgcgc aagaaatcgt ttttagacaa
     1381 gctgcgcgat ttgtttgatt gattttaagg ttcggaaaca agcagccgta tcggggaatc
     1441 tccctgatac ggctgttttt gtttgtttaa aaatagtttt tattttcaat ggggtatggg
     1501 gcagggtggg ataactgttt ttaactgttc tttttaaaac ttgacatcat ggcgcgatgc
     1561 caacaatatg tgaacgtctg ttgtcaaagg aagaataatg aataaatctt tatccgggtc
     1621 ggtagaagaa taccgcgaac tgacgctccg aggcatgata ctcggtgcat tgatcactgt
     1681 aatttttact gcgtccaacg tttacctcgg tttgaaagtc gggctgacct ttgcctcgtc
     1741 gattccggcg gcggtgattt cgatggcggt tttaaagttt ttcaaaggca gcaatatttt
     1801 ggaaaacaac atggtgcaga cccaagcttc ggcggcgggt acgctttcga ccatcatctt
     1861 cgtcctgccc ggtttgctga tggcgggcta ctggagcggt ttcccgttct ggcagacgac
     1921 gcttttatgt attgccggcg ggattttggg ggtgattttc accattccgc tgcgttacgc
     1981 gatggtggtg aaaagcgatt tgccttatcc ggaaggtgtg gcggctgctg aaattttgaa
     2041 agtgggcggt catgaagaag gggataaccg tcagggcggc agcggcatca aagagctggc
     2101 ggccggcggt gcgttggcgg gattgatgag cttttgcgcc ggaggtctgc gcgtgattgc
     2161 cgacagcgcg agttattggt ttaaaagcgg tacggcgatt ttccagctgc cgatgggctt
     2221 ttcactggca ttgttgggcg cgggctattt ggtcggactg acgggcggta tcgccatcct
     2281 gttgggcatt tcgattgctt ggggcattgc cgtgccgtat ttctcctcac acattccgca
     2341 accttccgat atggaaatgg cggcgtttgc gatgaagctg tggaaggaga aagtgcgttt
     2401 tatcggtgcg gggactattg gcattgcggc ggtttggacg ctgttgatgc tgctcaagcc
     2461 gatggtggaa ggcatgaaga tgtcgttcaa gagttttggc ggcggtgcgc ccgctgcgga
     2521 acgcgccgaa caggatttgt cgcctaaggc tatgattttt tgggtgctgg cgatgatgtt
     2581 tgttttaggc gtgtcgtttt accactttat cggcgattcg cacattacgg gcggcatggc
     2641 ttggcttttg gtggtcgttt gcacgctttt ggcttccgtc atcggctttt tggtcgccgc
     2701 cgcctgcggt tatatggcag gtttggtcgg ctcgtcttcc agcccgattt ccggcgtggg
     2761 catcgtgtcc gtcgtcgtta tttcactggt tttgctgctg gtaggcgaat ccggaggttt
     2821 gttggcggat gaggctaacc gcaaattttt gctggcactg actttgtttt gcggctcggc
     2881 agtaatctgc gtggcttcga tttccaatga caacctgcaa gacttgaaaa ccggctacct
     2941 gctcaaagcc acgccttggc ggcagcaagt cgccctgatt atcggctgta tcgttggtgc
     3001 gctggttatt tcgcccgtgt tggaactgct ttacgaagcc tacggcttta ccggcgcaat
     3061 gccgcgcgaa ggcatggatg cggcgcaggc tttggcagcc cctcaagcga ctttgatgac
     3121 gaccatcgcg tcgggcattt tcgcccacaa ccttgaatgg gtctatatct ttaccggcat
     3181 cgcgattggc gcggtgttga ttgccgccga tttggtgttg aaaaaatcat caggcggcaa
     3241 acttgccctg cccgtccttg cggtcggtat gggtatttat ctgccgccgt ccgtcaatat
     3301 gcccatcgtg gcaggcgcgg tgttggcggc ggtgttgaaa cacatcatcg gtaaaaaagc
     3361 ggaaaaccgt gaaggccgtc tgaaaaacgc cgagcgcatc ggaaccttgt tctccgccgg
     3421 tctgattgtc ggtgaaagtc tgattggtgt gattatggcg tttattattg ccttctccgt
     3481 gaccaacggc ggctcggacg cgccgctcgc gttgaatctg caaaactggg atgccgccgc
     3541 ttcttggctg ggcttggcgt tcttcgttac cggaatgatt atttttgcac ggcgcgtact
     3601 gaaggcgggt aggtagcctg tcggagaaaa tgccgtctga aacgttcaga cggcattttt
     3661 tatcgggaaa gtgtaaggcg gagcttttcg gcttgcgccc acgttttgcc ggcaaggtct
     3721 ttgggcgaca ggttcggctc gccttgtagc ggccagtcga tgccgacggt cggatcattc
     3781 caaattagtg agtgttcggc ttcagggttg taatagtctg tgcatttata gacgaactcg
     3841 gcttcatcgc tcagtacata gaagccgtgt gcgaaacctt cgggtaccca cagttggcgt
     3901 ttgttttctg cggacagaat ctcgcctacc catttgccga aagtggggga gtctttacgc
     3961 atatcgacgg ccacgtcgaa tacttcgccg acaactacgc gtacgagttt gccttgtgtg
     4021 ttttcggttt ggtagtgcag gccgcgcaat acgcctttgc cggatttgga gtggttttcc
     4081 tgcacgaagg tgcgttcgca gacttgggtt ttaaaccact cgtcgcggaa ggtttccata
     4141 aaaaagccgc gcgcgtcgcc gaagacttgg ggctcaagca gttttacgtc aggaatggcg
     4201 gtatcaatga tgttcatctt tttatctttc atctaaaggc cgtctgaaaa gtttcagacg
     4261 gcctcaaaca ttattttttc aacaggcgca gcaaatattg gccgtattgg tttttcgcca
     4321 tcgggcgcgc caattcttcc agtttttcat cggaaagcca accgttgcgc caagcgattt
     4381 cttccag
//
LOCUS       DAWEWP010000149         4369 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWEWP010000149 DAWEWP010000000
VERSION     DAWEWP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.149"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(35..1414)
                     /gene="norM"
                     /locus_tag="WGN53_001730"
     CDS             complement(35..1414)
                     /gene="norM"
                     /locus_tag="WGN53_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ0140695.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGTVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVLWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            1749..2900
                     /locus_tag="WGN53_001731"
     CDS             1749..2900
                     /locus_tag="WGN53_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0140696.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGTVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTSERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            3004..4302
                     /locus_tag="WGN53_001732"
     CDS             3004..4302
                     /locus_tag="WGN53_001732"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ0140697.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAILAYYNVQLQHLHNAEPVKAEDVMAVIEKVALRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
BASE COUNT         1045 a         1197 c         1249 g          878 t
ORIGIN      
        1 tgttaatcca ctatattttt aatcggcgga gttttcagac agccttatgc gatctgacca
       61 tctccctact gcacaattcc aagcaccaca ccaaggcgat ggcggcgatg gtgagcgagg
      121 caatcaatgc cgtccagaag ccgtaaatgc ccatatcgaa acggtaggcg agcaggtagc
      181 ccggcagcag accgcagccc caaaaggcgg cggcgtggat gaacatcggc acctttgtaa
      241 ctttgtagcc gcgcaaggcg taggaggcga tacattgggt gaagtctgcc ggttggaaca
      301 agccggcgaa cagtaagacg gtggcggcga tgcttaaaac cgccggatca ttgttgtaca
      361 tacttaccag cggcgaacgg aataatacca aggaaagcac ggtaatcacg gcgagcatcc
      421 atcctaacac cagtgacacg cccgaaatat aacgcgcccg cgaaaattcg cgccgcccaa
      481 gcgaaaagcc gatgcgcacc gtccccgccg agccgacgct ttgcggaatc atatagagaa
      541 tccccgacaa actgatgccg acctgctgcg ccgccacata atcctcgccg aaaggcgcaa
      601 tcaaaaacac gataaacgaa aacgcgctgg cttccaaaaa ataagacagc ccgatgggtg
      661 cgccgatttt ccaaatctgt ttgaacaccg cccaatccgg tttgccgaat ttcgccgtca
      721 gtccgaatgg gcggaagaaa ttttccttgg cgatataaat ccacaatgcc agcgcgctga
      781 accacaacac tgccatcgtc gccagtccgc agcctgcgcc gcccaaagcg ggcataccga
      841 atttgccgta aacgaaaata tagttcagcg gcacgttcaa cacaaacgcc gcaaagctga
      901 ccaacataat caggcgcggg cggttcaggc tggaggcgta ggcgtgcagt gcgcggtgta
      961 ccattgccgc cggcatcgcc aagctggtga acagcatata ctgcgccatt gtgccttcca
     1021 cataatcgct caaggtcagc cagttgcgga acggcgtaat cgccgcccac atcaataccg
     1081 tgccgaacac gcccaaaaac agcccgaacc aaatcccctg ccgccccgtt tcgcccactt
     1141 cgtcggtttt acccgcgccg taaagctggg caatcatcgg gttcagcgcc gccataatgc
     1201 ccataaaggt aatataaacc gtggcaaacg cgctgctgcc caaagccacc gccgccaagt
     1261 cttccttgcc tgcaccaccc gccatcacag tatcgataaa accgatgccc acctgcgcga
     1321 cctgcgccaa cagcatgggc agggcaaggg cggtcagcag gcggacttct ttcaggaaga
     1381 cggaaaagga aaagcggttg aggtcgagca gcataagtgt tcaatcaaca aaaatgccgt
     1441 ctgaagcaga aaacggcagc aggaagtgat gagaaataat ggtgcacatt atatcgtaaa
     1501 aaaatgctgt gccgtcagac ggcggataca gggtataaaa gtatattcag attgtgtgta
     1561 ttttatggta aagtttggtt ttaacgactt gacggcattg agccgtcgga caggggctgt
     1621 tcggattctg aatcggaagg aagcatcgcc gttttgacag cggcgtgatg cgttgcggca
     1681 aagatgccgt ctgattgcgg atcgggcagc cttttgtatt tacatgataa aatagaaggc
     1741 agattctcat gcagacatgg cagcttccgg aacatatcgc cgacgtactg cccacgaacg
     1801 cgcggcagct tgaaagcgcg agggagcagt tgttggcact gttccgcgta cacggttatg
     1861 aactggtaca gcctccgctg atggagtacg cacattccct gctgacgcat atcgatgcgg
     1921 ggctttccct gaaaaccatt ttggtaacgg acaggctcag cggcaggcag ttgggcatac
     1981 gcgccgacat cacgccgcag gtggcgcgta tcgatgccca tcttttatcc gccaaccaag
     2041 ggattaatcg gttgtgttat gccggtccgg tgttgcacgc gcagcccgac ggtctgctga
     2101 atatgcgcga acccttgcag gcaggggcgg aaatgtacgg ttttgccgac atccgtggcg
     2161 acatcgagct gatagacctg atgctgaaaa gcatgaaaat tgccgatatg ggcaaagtgc
     2221 tgctttcgct ggggcatatc ggcatatttc gcgccttgtc cgatgcggcg catttggatg
     2281 cggggcagtc tgcaacgctg cttgccttga tgcaggataa agataccggg acggtcgaag
     2341 cgcaggtcaa ggcttggaag ctggacggca tgtgggcaaa agcattctcg ctgctgccgc
     2401 gcctgtacgg cgggcgtgaa gtgttgtccg acgcgcgcgg acggttgccg gatttgtcgg
     2461 cggtcggcgg cgcgttagac gaattgcagg cggtgtgcga cgcattcccc gattgtgaaa
     2521 tccatatcga cttgtccgag ctgcgtgtcg acaattacca cacgggcttg ctgtatgccg
     2581 cctatgccgc cgatttccac gacgcggtcg cgcgcggcgg gcgttatgac ggattgggcg
     2641 gatatttcgg cagggcgcgc ccggcaacgg gattcagttt cgacttgcgc agctttatcg
     2701 ggcgtttgcc caccagcgaa cggcagcccg ccgtgttggt cgatgcggaa gatgccgaag
     2761 cggcgcgcga agcagtcgaa gccttgcgtg aacaagggca gtgtgtcgtg attgactacg
     2821 gtatcggaca caatgtttcg gaagagcttg caggccgtct gaaaaagacg gacggcgttt
     2881 ggcaggtcgt gaaacgctaa atacccgttc atggcggatg aaaggcaaat cgtggtgggg
     2941 cgcaaagccg caccggtttg gggatttccg caataatttt taatatcgat aggttatatg
     3001 gctatggcta aaaatgttgt agtaatcggc gcacagtggg gcgacgaggg taaaggtaaa
     3061 atcgttgact ggctggcgga agaagccggc ggcgtggtgc gcttccaagg cggccacaat
     3121 gcgggtcata ctttggtcgt cggcggcaaa aaaaccattt tgcgcctgat tccgagcggc
     3181 atcctgcatg aaagtctgga ctgcttcatc ggttcgggcg ttgtcgtctc ccccgaagcc
     3241 ctgttgggcg aaatcgacga gttaaacgct gcaggcgtga aaaacgtcga aggccgtctg
     3301 aaaatcgcgc cgacctgccc gctgatcctg ccttaccaca tcgcgctcga ccaagcccgc
     3361 gaagcatcgc gcggcaaagg caaaatcggc acgaccggcc gcggcatcgg ccctgcctac
     3421 gaagacaaag tggcacgccg cgccattcgc gccgccgatt tgctgcatcc tgaaaaactg
     3481 cgtgaaaaac tggatgccat ccttgcctat tacaatgtcc aactgcaaca tctgcacaat
     3541 gccgagccgg ttaaagcgga agacgtgatg gcggttatcg aaaaagtcgc gctgcgcatt
     3601 gcgccgatga ttaccgacgt gtcccgcgtg ttgaacgaga aaaacaaaaa cggcgaaaaa
     3661 ctgctgtttg aaggcgcgca aggtgcgttg ttggacatcg actacggcac ttatcccttc
     3721 gttacctcgt ccaactgtct ggcgggagcc gcttccgcag gcgcgggcgt aggccctcaa
     3781 atgctggatt atgttttggg catcgtcaaa gcctatacca cgcgcgtcgg ttcaggcccg
     3841 ttcccgaccg aattgttcga cgaagtaggc gcaggtttgg cggaacgcgg acacgaattc
     3901 ggctcggtaa ccggccgcgc acgccgctgc ggctggtttg atgccgccgc cctgaaacgc
     3961 tccatccaaa tcaacggcat ttccggtatg tgcattacca aactcgatgt aatggacggc
     4021 gttgaaacca tcaatatctg cgtcggctac gaattgcccg acggcggcaa aaccgatatc
     4081 ctgccttgcg gttccgatgc ggtggaaacc tgcaagccga tttacgaaac catgcccggc
     4141 tggcgtgaat ccactttcgg cgtgaaaagc tacgacgcat tacctgccaa tgccaaagca
     4201 tatttgaaac ggattgaaga agtctgcggt gcgccggtcg ccatcgtctc caccggcccc
     4261 gaccgagaag aaacgattgt gctgcatcat ccgttcgcat aaggttttgc agtaaaaatg
     4321 ccgtctgaag cccctccgag ttcagacggc atttttgctg ccgttttat
//
LOCUS       DAWEWP010000150         4317 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWEWP010000150 DAWEWP010000000
VERSION     DAWEWP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.150"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..1175
                     /locus_tag="WGN53_001733"
     CDS             <1..1175
                     /locus_tag="WGN53_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0140698.1"
                     /translation="IQARGYQYDTETDKFVKGYEYSNCLWYEDERRINRTYGCYGVDS
                     SIMRLMSDYSRFPEVKELMESQMERLARPYWEKLRNRPDMYYFKNYNFKRCYFGLNGG
                     DCLVAKGDDGRTFISFSLQGNSKYKEEMDAKKLEEILSLKVDANPDKYIKATGYPGYS
                     EKVEVAPGTKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEAP
                     NAQPLPEVSPAENPANNPAPNENPGTRPNPEPDPDLNPDANPDTDGQPGTSPDSPAVP
                     DRPNGRDGKDGGLLCKFFPDILACDRLPEPNPAEDLNLPSETVNVEFKKSGIFQDSAQ
                     CPAPVTFTITVLDSSKQFAFSFENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            1478..1762
                     /locus_tag="WGN53_001734"
     CDS             1478..1762
                     /locus_tag="WGN53_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ0140699.1"
                     /translation="MKLLAALIPLLMSVVGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            1763..2950
                     /locus_tag="WGN53_001735"
     CDS             1763..2950
                     /locus_tag="WGN53_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ0140700.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGSKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIILLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEHQAVFQDKTEGEPVNNGNLTADMFVPTLSE
                     KPESKPIYNGVRQVRTFEYIAGCVEGGRTGCTCYSHQGTALKEITKEMCKDYARNGLP
                     FNPYKEESQGRDVQQSEQHHSDRPQVATLGGKPWQNLMYDNWQERGKPFEGIGGGVVG
                     SAN"
     gene            3165..4121
                     /locus_tag="WGN53_001736"
     CDS             3165..4121
                     /locus_tag="WGN53_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0140701.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         1403 a          961 c          978 g          975 t
ORIGIN      
        1 acatacaggc acgaggctac caatacgaca ccgaaaccga caaatttgta aaaggctacg
       61 aatatagtaa ttgcctttgg tacgaagacg aaagacgtat taatagaacc tatggctgct
      121 acggcgttga cagttccatt atgcgcctta tgtccgatta cagcagattc cccgaagtca
      181 aagaattgat ggaaagccaa atggaaaggc ttgcccgtcc gtattgggaa aagttaagga
      241 atcgtcctga tatgtattat tttaaaaact acaattttaa acgttgttat ttcggattga
      301 acggcggaga ttgtttagtt gctaaaggtg atgatggtag aacttttatc agtttctcac
      361 ttcaaggaaa ttcaaaatac aaagaagaaa tggatgccaa aaagctggaa gagattttat
      421 cgttgaaagt cgatgccaat cccgacaaat acataaaggc aaccggatat cccggttatt
      481 ccgaaaaagt agaagtcgca cccggaacaa aagtgaatat gggacccgtc acggacagga
      541 acgggaatcc cgttcaggtt gtcgcaacat tcggcaggga ttcgcaaggc aacaccacag
      601 tggatgttca agtaattccg cgtcccgact taacccccgg aagcgcggaa gcgccgaacg
      661 cacagccgct gcccgaagta tcgcccgctg aaaaccccgc aaacaacccg gcccccaatg
      721 agaaccccgg cacgcgtccc aatcccgaac ccgaccccga tttgaatccc gatgcaaatc
      781 ccgatacgga cggacagccc ggaacaagcc ccgattcccc ggccgttccg gaccgcccaa
      841 acggcaggga cggcaaagat ggcgggcttt tgtgcaaatt cttccccgac attctagctt
      901 gcgacaggct gcccgagccc aatccggcag aagatttaaa tctgccgtct gaaaccgtca
      961 atgtagagtt taagaaatcc ggaatctttc aagattccgc acagtgtccc gcccccgtta
     1021 cgttcactat aaccgtgctt gattcaagca agcagttcgc gttcagcttt gagaacgcat
     1081 gtaccatagc cgaacggcta aggtacatgc ttctcgccct tgcttgggcg gttgccgcct
     1141 ttttttgtat ccgcacagta tcccgtgaag tctagcaggc gcagcaccgc cgggcttcag
     1201 taacttgtgc caaggcaggg ggaggacgtc cagaaagatt tgtaaagacg gctttatcgt
     1261 ctttataaat ctttttggat accccttgcc gccccgccaa aagaacacac tctgctgcaa
     1321 gggcaggtgg taaggcgcgc gctttttgcg ccgtccccct gcccccgcag cgtcgcaagt
     1381 gagactgggg gtgtgggggc tagtccccgc aaaatctttc agattaagaa acattttttt
     1441 aatgaggcaa ccgtgccttt taagaaaggg atagcaaatg aaattgttgg ccgcattgat
     1501 tccgctcttg atgagcgtcg taggccgtat attgactgca ttgggattga tggccgtgac
     1561 ctattcgggg gtggatagat tggtagccca ttttcaacag gcgataaccc atagcataac
     1621 gggcgcacct caagcaatgt tacagctttt ctatataagc ggcggtggta ctgttctaaa
     1681 cattcttttc ggcgcgatcg cctttattct gtcattcaaa caaatgacaa aactagcaac
     1741 ctcaatcggg aagaaaaaat aaatggcaga gatctgtttg ataaccggca cgcccggttc
     1801 agggaaaaca ttaaaaatgg tttccatgat ggcaaacgat gaaatgttta agccggatga
     1861 aaacggcata cgccgtaaag tatttacgaa catcaaaggc ttgaagatac cgcacaccta
     1921 catagaaacg gacgcgaaaa agctgccgaa atcgacagat gagcagcttt cggcgcatga
     1981 tatgtacgaa tggataaaga agcccgaaaa tatcgggtct attgtcattg tagatgaagc
     2041 tcaagacgta tggccggcac gctcggcagg ttcaaaaatc cctgaaaatg tccaatggct
     2101 gaatacgcac agacatcagg gcattgatat atttgttttg actcaaggct ctaagcttct
     2161 agatcaaaat cttagaacgc ttgtacggaa acattaccac atcgcttcaa acaagatggg
     2221 tatgcgtacg cttttagaat ggaaaatatg cgcggacgat cccgtaaaaa tggcatcaag
     2281 cgcattctcc agtatctata cactggataa aaaagtttat gacttgtacg aatcagcgga
     2341 agttcatacc gtaaataagg tcaagcggtc aaaatggttt tatactctgc cagtaataat
     2401 attgctgatt cccgtttttg tcggcctgtc ctataaaatg ttaagtagtt atggaaaaaa
     2461 acaggaagaa cccgcagcac aagaatcggc ggcaacagaa catcaggcag tatttcagga
     2521 taaaacagaa ggcgagccgg taaacaacgg taaccttacc gcagatatgt ttgttccgac
     2581 attgtccgaa aaacccgaaa gcaagccgat ttataacggt gtaaggcagg taagaacctt
     2641 tgaatatata gcaggctgtg tagaaggcgg aagaaccgga tgcacatgct attcgcatca
     2701 agggacggca ttgaaagaaa ttacaaagga aatgtgcaag gattacgcaa gaaacggatt
     2761 gccgtttaac ccatataaag aagaaagcca agggcgggat gtccagcaaa gtgagcagca
     2821 ccattcggac agaccgcaag ttgccacgtt gggcggaaag ccgtggcaaa atcttatgta
     2881 tgataattgg caggagcgcg gaaaaccgtt tgaaggaatc ggcgggggcg tggtcggatc
     2941 ggcaaactga agaaaacggc aagagagaaa aaagacccgt aaaccgtttg aatatagacg
     3001 gcttacgggt ctttgtttcg cgcaaagcaa gggctaaggc agtcaggcag caaatcccgc
     3061 aatgtattaa aacagacgcg tagaaatgcc ggctgccttt atccatcctc aaaattgaat
     3121 atcatcctag ccgtatcaag gctgtataaa taaggaaaat accaatgaat ataatcgggc
     3181 tggacatctc aaaggatacc atagacgcaa cattgcataa aacaaacgga agtatccatt
     3241 acattaaatt taagaataat gatgatggat taaaacagtt tagattgtgg ataaagggaa
     3301 acagaatcag aaaagcctat atcggcatgg aggcaacagg catctattac gaaaaggcag
     3361 cagatatgct ttcttcctac tataccgttt acgttatcaa tcccttaaaa atcaaggact
     3421 acgggaaaag caggtttaac cgtaccaaaa ccgacaaagc agattcaaac ctgatagcag
     3481 attacataaa aaggcatcaa gatacattga taccgtatca gatacccaaa aacaaagcac
     3541 tgcaaaaact gattaacctt aaaaatcaat tacagcaaca tcagaagcaa attaaaaacc
     3601 gtcttcatag cactgaagaa gacttcataa ggaacataca tcaagacttg atagatacca
     3661 tacaggacaa gatggaacag gtaaaaatag ccatatccga acaaatcaaa aaacaaacgg
     3721 acaataacca ttaccgcaat cttcaaacca tcccgagcat aggcaaagac accgcatcag
     3781 ttctttatgc gcaactgaca gaaaaacatt ttaaaaccgc aaaccagttt gtatcctatg
     3841 ccggattaag tcccgccatc atacaatcag ggacaagcgt aagaggtcgg ggcagattga
     3901 gccgatacgg aaacagacga ttaaaaagta cgctgtatat gcccgccctt tgtgcttacc
     3961 gttttaacgc atttccgaaa ttaataaata atctgaaaaa agcgggtaag ccaaagatgg
     4021 taatcatcgt tgccatcatg cgcaaactgg cgaagctcgc ctattacatt gttaaaaccg
     4081 gccagcctta cgatgcggaa agacaccgat tgaatcaata aaattcaaca aaattaaacg
     4141 gttacgcgaa tatatttgtg taaccgtgca tttgcatatc gtaaataaac gtaaataaaa
     4201 ataacaatat aaatcagtat attgcaactt tgttttttat tttgtgttga cgggcaacat
     4261 atcatcatct gcgcgggaat gacggcggag cggtttctgt tttttccgat aaatgcc
//
LOCUS       DAWEWP010000151         4307 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWEWP010000151 DAWEWP010000000
VERSION     DAWEWP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.151"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            94..365
                     /locus_tag="WGN53_001737"
                     /pseudo
     CDS             94..365
                     /locus_tag="WGN53_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            405..1439
                     /locus_tag="WGN53_001738"
     CDS             405..1439
                     /locus_tag="WGN53_001738"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ0140702.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPSADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1645..2226
                     /locus_tag="WGN53_001739"
     CDS             1645..2226
                     /locus_tag="WGN53_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ0140703.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2295..2477
                     /locus_tag="WGN53_001740"
     CDS             2295..2477
                     /locus_tag="WGN53_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ0140704.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            2474..3235
                     /gene="kdsB"
                     /locus_tag="WGN53_001741"
     CDS             2474..3235
                     /gene="kdsB"
                     /locus_tag="WGN53_001741"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ0140705.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSSLETIESLEQLRVL
                     WHGYSIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            3258..3575
                     /locus_tag="WGN53_001742"
                     /pseudo
     CDS             3258..3575
                     /locus_tag="WGN53_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            3680..3877
                     /locus_tag="WGN53_001743"
     CDS             3680..3877
                     /locus_tag="WGN53_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140706.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            4034..4126
                     /locus_tag="WGN53_001744"
     tRNA            4034..4126
                     /locus_tag="WGN53_001744"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4068..4070,aa:Ser,seq:gct)
BASE COUNT         1075 a         1039 c         1166 g         1027 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gctaaggcga gccaacgccg tactggtttt
       61 tgttaatcca ctataaatga aagatactgg caaatgaaag agatgaaacc tgtccgttat
      121 catattggcg atatgcccga gacttcaaaa caaaccgcct cccgtcatga cgacagggca
      181 gtgggtgttg acgatgattg ttttcatgat tcctttggtc aatttttgtt tgggtgttcg
      241 gcagaggcaa cccgaaccgc gccaatttct gtaaagcgca gttgcttatt tacctgattg
      301 gttcgcttat cggtttggtc ttcgcgttgt ttataggtgg gtctgtatcg ggtacgcatg
      361 attaatgccc cgggctgatt ttgcttcgag gatttgtatc gaatatgccg aattgtttca
      421 aatttcatac cgttatcgaa cggcattggc aaaaacctta tccggttttg tcttttctgc
      481 ttaagccgct ctccgggctg tttgccaaaa ttgcggcaaa acggcggacg gattttttat
      541 cgggaaaacg gcaaagcgaa aagctgcccg tgcctgtggt cgtggtcggc aatattcacg
      601 cgggtgggac ggggaaaacg ccgattgttg ccgcgctggt gtcgggtttg caggaaaagg
      661 gcgtcaaggt cggcatcatc agccggggct acgggcgcaa gagcaaggcg gttcatgtat
      721 tgaatgctga gagccgagcg gaagatgcgg gcgatgagcc tttgctgctg ttccgcaaaa
      781 ccggtgcgcc gacggcggtg ggcagcagcc gtgcagaggc aggcagggcg ttgctggcgg
      841 cgcatcccga catcggactg attgtggcgg acgacggttt gcagcattac gccctgcggc
      901 gagatgtgga aatcgcggtg tttccgtcgg cggatacggg gcgcacggat ttggatttac
      961 tgcccaacgg cagtttgcgc gaacctttgt tgcggctgga ttcggtggat gcggtcgtcg
     1021 tcagcggcgg caaggcggat gcgctgttta ggccgtctga aaatatgttt cacagccgta
     1081 tcgaagcggg acggatttac cgtttgaaca atccgtccga aatactggac acaggccgtc
     1141 tgaaaaatca aaccgtcgtc gccgtggcag gtattgccaa gccggcgcgt tttttcgatt
     1201 cgttgcggaa tatgggcatt accgtgaagc gaaccgtcgc gctgcctgac cacgccgaca
     1261 tttcggcggc agatttgccc gatgcggacg cggtcattat tacggagaaa gatgcggtca
     1321 aattttcaga cggcatttgc accgataatg tttgggtgtt gcccgtttgt gcgataatcg
     1381 aacctgattt ggcggcgttt gtgttggagc ggttggaaga tgtaccgaag gccgtctgaa
     1441 agcacggttt gggcggagtg attacggatt tgaataagaa cgcctcgcgc cgtcattccc
     1501 gcgcaggcgg gaatctaagt ctcgaatttt caggaatgcc taggaggctt cagaaatccc
     1561 aaatctccgg atttccactt ggacaggaat gagaaaatcg gtcgtatttt ttatctgcat
     1621 taatcattcg ttaaagtatt gaatattaaa ctgaaaacct tgttattgcc cttcgccacg
     1681 ctggcattgt gcaccaacgc ttttgccgcc ccgcccagcg acgcgtcgtt ggcgcgttgg
     1741 ctggatacgc agaattttga ccgggatata gaaaaaaata tgattgaggg ctttaatgcc
     1801 ggatttaaac cgtatgcgga caaagccctt gccgaaatgc cggaagcgaa aaaagatcag
     1861 gcggcaaaag cctttaaccg ctatcgtgag aatgttttga aagatttgat tacgcccgaa
     1921 gtgaaacagg ctgtccgcaa tactttattg aagaatgccc gtgagatata cacgcaagaa
     1981 gaaattgacg gcatgattgc cttttacggt tcgcctgtcg gtcagtccgt cgttgccaaa
     2041 aatccgcgct taatcaagaa atcgatgagt gaaatagcag tatcttggac tgcattgtca
     2101 gggaaaatcg cgcgacatca tctgcccgcg tttacggaag agttgcggcg catcatctgc
     2161 ggcggtaaaa atcccgatgc gggctgtaaa caagccggac aggttgggaa aaggcatcag
     2221 aaataaatga tagccgtctg aaatattgaa gagggcatcc gattgattga accatcaaac
     2281 ccgaaagcaa ccctatggaa aaaaaattct tagacatcct cgtctgcccc gttaccaaag
     2341 gcaggctgga atatcatcag gacaaacagg aattgtggag ccgtcaggcg aagcttgcct
     2401 atccgattaa agacggcatt ccctatatgc tggaaaacga agcgcgagcg ttgagcgaag
     2461 aggaactcaa agcatgactg aattcgtcgt attgattccg gcgcggctgg attcatcgcg
     2521 cctgcccgga aaagccttgg cggatatcca cggtaaaccg atggtcgtgc gcgttgccga
     2581 acaggcggca aaaagtaaag ccgtgcgcgt cgtcgttgcc accgaccatc ccgatattca
     2641 gacggcctgt caggcgcacg gcgtcgaagt cgtcatgact tcaaaccggc acgaaagcgg
     2701 cacgacgcgc cttgccgaag cctctgccgc gctgaagctg ccgccgcatc tggtggttgt
     2761 caacgtacag ggcgacgagc cgctgattgc ccccgaactc atcgaccgca ctgccgaagt
     2821 cttggtggaa aacaacgtac agatggcaac tgcggcacac gaattgcacg atttcgacga
     2881 attgatgaat cccaatgccg tcaaagtcgt cctcgacaaa aaccgcaacg ccatctactt
     2941 cagccgcgcc ccgattccct atccgcgcga tgcgatgcgt gcaggaaaac gcgaaatgcc
     3001 gtttgaaacc gccgtcctgc gccatatcgg catctacgcc taccgcgtcg gcttcctgca
     3061 acgctacacc gaaatgagcg tttcgtcgct ggaaaccatt gaatcgctgg agcaactgcg
     3121 cgtcctgtgg cacggctact cgattgccgt cgaaaccgcc aaagaagccc ccgccgccgg
     3181 tgtggatacg caggaagatt tggacagggt ccgcgccgta tttcagaccg tataaaacag
     3241 gttcaaaggg aaaagatatg cagcaacata ttgaaaagtg gcagcactta agccgtgaag
     3301 aacagaaaat ccttgccgaa gtatggggtc tcgtgcaaaa cgatgatcag gaggttcact
     3361 atgaaatgct taaattgaac gcacccgacg aagccagcgg cgaattttgg ttcagaatgg
     3421 cagaaacact cagcaccctg ccgcccaacc gttccctcga ccttagaatg aacggcggca
     3481 ggctggcgac cgccgtatcc atcctttccg tcatgattga agacaatccc gacataccgc
     3541 agctttgggc gcaaaaaatt accgcgctca attatagtgg attaaattta aaccagtacg
     3601 gcgttgcctc gccttgtcgt actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     3661 ttttgttaat ccactatatt tggcacacgg gcacaaagcc cgtgccgacg gtttggcaca
     3721 acagcccgac aaagcggcag aagccaacga ggaggaatac ctgaccaaag ccctgtcgca
     3781 aaacctgctg tcaacattgg atgccgcgct tgcacgtttt cctgaagacg cgtggtttca
     3841 ggaaatcaaa caggatgcac aaaagcattt tgcttgagga tgtggcagtc aggaatattt
     3901 ccattcagga agaaaagaag tgcctgattg ggtataatca gggtaaatct tattttattt
     3961 caaaagatta atatttgctt tctgtttttc cttgacggta tcggaaaagt tgattatagt
     4021 tacagcttcc ttaggagtaa tggctgagag gctgaaggca cttccctgct aaggaagcat
     4081 gtggggtcaa cctgcatcga gggttcgaat ccctcttact ccgccagata aaaaatagac
     4141 gctgtgtttt acagcgtcta tttttttatg caattttata gcgggttggt gcaaaaccag
     4201 tatggtattg ccctgtcttg attctgaatt ttgttatagt ggattaacaa aaaccagtac
     4261 ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctg
//
LOCUS       DAWEWP010000152         4294 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWEWP010000152 DAWEWP010000000
VERSION     DAWEWP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.152"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(60..1316)
                     /locus_tag="WGN53_001745"
     CDS             complement(60..1316)
                     /locus_tag="WGN53_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ0140707.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWNEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLQSLKIDL
                     DTGKYLRLDSAKHQTDGFFAAVLQRK"
     gene            complement(1352..2209)
                     /locus_tag="WGN53_001746"
     CDS             complement(1352..2209)
                     /locus_tag="WGN53_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ0140708.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQSFHAFEPPLNPYGWRGIYIQD
                     WAEYGFERQEVRYHLLDRMAYLAPARAAETETLNETEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(2270..3085)
                     /locus_tag="WGN53_001747"
     CDS             complement(2270..3085)
                     /locus_tag="WGN53_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0140709.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            3149..3319
                     /locus_tag="WGN53_001748"
     CDS             3149..3319
                     /locus_tag="WGN53_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140710.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            3329..4114
                     /locus_tag="WGN53_001749"
     CDS             3329..4114
                     /locus_tag="WGN53_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ0140711.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT          883 a         1050 c         1304 g         1057 t
ORIGIN      
        1 gatgaaatgc cgtctgaacg ctttgaaaag ggttcagacg gcattttgtt taaacagggt
       61 tatttgcgtt gcaatacggc ggcgaagaag ccgtcggttt ggtgttttgc cgaatcgagg
      121 cgcaggtatt tgccggtatc caaatcgatt ttcaggcttt gcagaagttc ggcgcagttg
      181 acgggttcaa attcgggatg ttcggacagg aaacgttcga cttgcagctc gttttcttcg
      241 ggcaggatgc tgcaagtggc gtaaaccaag cggccttgcg gttttaccag tttggaggca
      301 gcatcgagga tgctgtgttg ctgttccaaa aggttggcca cggtctcggc agattggcgg
      361 tatttgaggt cgggattgcg gcgtaaagta cccaaaccgg agcagggcgc gtccaccaac
      421 acacggtcgg ctttgcctgc cagtcgggcg atacggctgt cgtgttcgct gctgatgcgt
      481 tcggggtgga tattggtcag tccggcgcgg gtcatacgcg gtttgaggtt ggcaaggcgt
      541 ttttcggcga tgtcgaaggc gtagattctg cctttattgg ccatttgcgc accgacagcc
      601 aaggttttgc cgccggcacc ggcacagaaa tcgacaatga tttcgccgcg ttttgcgccc
      661 accaataaag caagcagctg gctgccttcg tcttggactt ccagtgtgcc gtctaaaaac
      721 agttcgtgtt tgttaagcgc gattttgttt ttcaggcgga tgccccaagg cgaataaggc
      781 gttgcctctg catcggcact ttcggcttgc aacagcggca gcactttatc gcgtttgcct
      841 ttcaaagtgt tgacgcggat gtcgagcggc gcggcttggt tgatgctgcg tccgaaggcg
      901 aggatttctt cttcgttcca atgctgttgc agttgttcga ccagccattg cggcaattct
      961 gcggcggtat tcagaccgtc tgaaaactcg gttttgcggg ctttcaaatt gccgaggaac
     1021 tctgtttctt cttcatcaag caggtctttg atttggctga tgttggtgct tctgccgaga
     1081 accagtgcgg cgagtgcggc tttgcgtggc tgcgcgtgag gacggcgcag ggcagtgctg
     1141 attttttgat agtggcgcag cgcggcaaag gcggtttcgg cgatttcgtg gcggtcttgg
     1201 cggccgagct ttttatgttc gcggaagtag gcggagagga cggcatcgga aggctgtttg
     1261 aaagtcagca tttcagccaa aactttggcg gtatggtcga gttgtgcggc gttcataatg
     1321 taatgggttc tcttaaagtt ggcaaagggt ttcagacggc cttcataatg cgttcgattt
     1381 cgtgccaaaa gccgtccggc cgacattcca aaacggcaat caagccttgg tcgatacggc
     1441 ggatttcggt ttcgttcaat gtttcggttt cggcggcacg cgcaggtgcg aggtaggcca
     1501 tgcggtcgag cagatggtag cggacttctt ggcgttcaaa cccgtattcc gcccagtctt
     1561 gaatatagat gccgcgccag ccgtatgggt taagcggcgg ctcaaaagca tggaaacttt
     1621 gtggaaaaaa tccgatgccg cgcacgatgg aaacgggttt tacgtcaagc ggcagaccgt
     1681 gttgccgcaa ggttcggatg ccgtctgaag tgtgcggcag accgagttgg gcggtcagtt
     1741 tggcggcttt tcccaacagc gtgtctttgg ggtcgagtcc gcgcatgtct tcgagcctgc
     1801 ccgtgccgcc gccgtaatat ttgcaggtca gctcgatatg gtagggcttg ccgttaagcc
     1861 ttaccacaaa atccgccgcg ccttgcgtat tgccgtccga accggaaacc gtgaggttgt
     1921 gcgcgagcag ttcggcgtgt ggcgcgttgg caaaccaaaa tgccagcagt tcttcggcgt
     1981 aaatgcccaa gcggtgtccg aacggggcgc gtcgggtgag gtgttccgtc agcggcgcgg
     2041 gatcggcatc caatgccaaa agatagcgga aaccgcgttc ccccaatagt tcgcgcacgc
     2101 ccaattcgca accgctttgc cacaaaggcg gcgcagtcag cagcgaggcg aggtcgcgga
     2161 cgggttggct ggtgagcttc caccataatg cgtctagggc gtaattcatg cttatccgaa
     2221 tatcagtcat gccgtctgaa aagctggaaa tgttttcaga cggccttaat cagtcttttt
     2281 tatggatacg atggctgacg gtattgaaaa aaccgcgcag gatatcgatg tcttcggttt
     2341 gcgtgttggc gcgtccgaac aggctctgca tacggcgcat cagacgctcg ccgttgcggc
     2401 ggttgaaaaa gccgatgtcg ttcatcacgc tttccatgtg ggcgaccatg cctttgattt
     2461 gctcgtgggt cgcagcgtgg tcttcttgtt gaagatgggt catgggcgaa ccggtttggc
     2521 tgaagatttc gtagcacacg acctgcacgg cttgggcgag gttgagcgag aaatagtcgg
     2581 gattgccgtt gatggtcatc agtcggttgc aggcttggac ttcttcgatg ctcaagccga
     2641 aagtctcgtt gccgaaaacc agtgccactt tctcgcctcg gtttgcggtc tgcagtaatt
     2701 cggataccaa atcgcgcggg gtttgcagcg gcgcagtaat ttcgcggcgg cggctggtca
     2761 gggcgcaggc gatggtggtg tcggcaaggg cttcgtccaa agaagcggca atggtggcat
     2821 tttccaaaac gtctgccgcg ccggaagcga ggatgaagct ttcttccggt aatttaaacg
     2881 attgaggatg ctccgggtca aacacgggcg ggttttccgt catcggcgtt gccatcagat
     2941 ttggggcgac gatggtcagt ttgtgcagac ccattgtttt catcgcgcgc gcggcagagc
     3001 cgatgttggc gggatggctg gtgcgcgtga ggatgatgcg gatgttgtcc agataagcgg
     3061 gcagggcggg tttgagagta gtcatgttcg gttttcggtc ggaatggaat agggtataat
     3121 gcgccgtctt tttcgttcgg acggtcaaat gccgtctgaa atgttcttta acaacgtcgg
     3181 aaatttaacg ttgcgcctga agcagacacg cgtcgggcgg tattttaccc gatttggcag
     3241 gatttacccc aaccaaataa actccgattt tgccgctccg aaggactgcg cgtccgaacc
     3301 ggcggtatgc cacccttaag gaaatctgat gaatccgttt ttgaatacag cctttaaagc
     3361 cgcccgccgt gccggtcaga tgatgatacg cgccgcgggc aatctcgatg ccgtcaaaac
     3421 cgacagcaaa gcattcaacg attttgtttc cgatgttgac cgcaattccg aaatcatcct
     3481 ggttgaggct ttgaaagaag cctatccgca ccacaaaatc acttgcgaag aaagcggctc
     3541 ccacggtaaa gccgcagccg aatacgaatg gattatcgat ccgctcgacg gcacgaccaa
     3601 cttccttcac ggccatcccc aatacgccat ctctatggca ctcctgcaca aaggcgtgtt
     3661 gcaagaagct ttggtgtacg cccccgaacg caacgacgtt tacatggctt cgcgcggcaa
     3721 aggcgcgttg ctcaatgacc gccgcatccg cgtttccaac cgcatcgaat tgaaccgctg
     3781 cctgatcggt accggctttc ctgttgtcga tcaaagcatg atggacaagt atctggcgat
     3841 tttgaaagat ttcttggcaa aaaccgccgg cggccgtcgt gaaggcgcgg cttctttgga
     3901 tttgtgtgct gtggcgactg gtcgttttga cggctttttc gagttcaacc tcaaaccttg
     3961 ggatattgcc gcaggcgcat tgattgttca ggaagcgggc ggtatcgtaa ccgatatgac
     4021 gggtgaagac ggctggctgg aaagcggcga tattgttgcc gccaatccca aagtgctggc
     4081 gcaaatgtta aaaatcattt ccgcacacgt ttaaatttca gcccgctccc gggaatgcgg
     4141 gaaaacgggc gggtatgccg tctgaagtat cgcgccgata tttcagacgg catttttact
     4201 ggcagcgggg gcaggaaggt ggaaacggct aaatgcccga aactgccgaa gatggaaacc
     4261 catatctgat taaacggaga accctgtacg attt
//
LOCUS       DAWEWP010000153         4260 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWEWP010000153 DAWEWP010000000
VERSION     DAWEWP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.153"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            537..2264
                     /gene="ilvB"
                     /locus_tag="WGN53_001750"
     CDS             537..2264
                     /gene="ilvB"
                     /locus_tag="WGN53_001750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ0140712.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQEFMPSEDALGKWWKTIEEWRSQDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            2275..2766
                     /gene="ilvN"
                     /locus_tag="WGN53_001751"
     CDS             2275..2766
                     /gene="ilvN"
                     /locus_tag="WGN53_001751"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ0140713.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2833..3126
                     /locus_tag="WGN53_001752"
     CDS             2833..3126
                     /locus_tag="WGN53_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ0140714.1"
                     /translation="MSNIKIVALVTVKPEYTETLVAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            3206..4219
                     /gene="ilvC"
                     /locus_tag="WGN53_001753"
     CDS             3206..4219
                     /gene="ilvC"
                     /locus_tag="WGN53_001753"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ0140715.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1105 a         1015 c         1077 g         1063 t
ORIGIN      
        1 tcaatatatt aagtcgttgt tagccctctt ggttctgtca atgccgtcca atacagtccg
       61 gtgcaatgag ccattttcag gaagtatggt tgggaatatt tttgatatat caaaaataaa
      121 atcaagttca atcaatatcc cgagtcttta ttgggatgaa accctgtaaa ttttacaatt
      181 cttgatatcg aagaagggtt tttcacatct gcccctccta cacaaactgt caacaatttc
      241 catctttcaa aaaaggtact cctgatttta ttcttttaaa atcaatcaat aatttctctg
      301 ctttcaaaaa atattaaaag atgaaagatt tttcttgatt gaactgtcgg tttgcgttat
      361 cgtttctact ctttttcaca aactctgtgt tcctttccaa ttgattggat aatccgtctg
      421 ttgccgtgtt cccccaatgc gcgtacccta aatcgctgct tggtggaatt gcattacagg
      481 tgctgtggca aagcggtttg tccctattgg tttgaaaccg tataaaagag gtcgttatgc
      541 agttatcagg cgcgcaaatc atagtgcaga gtctcaaagc cgaaggtgtc gagtacgttt
      601 tcggttatcc cggcggtgcg gttatcgaaa tctacgatgc ccttttccaa ctcaataaat
      661 tcaagcacat tctgacccgt cacgagcagg cggcagtaca cgcggcagat gcgtatgcgc
      721 gcgtcagcgg taaggtgggc gtggcattgg ttacatccgg cccgggcgtt accaatgcac
      781 tgaccggtat tgctactgcc tatacggatt cgattccgat ggtggtcatc agcgggcagg
      841 taggcaattc cctgattggt acggatgcgt tccaagaagt tgatacggtg ggtattaccc
      901 gtccgtgcgt caaacacaat ttcctggtta cggacatcaa tgagttggcg gaaaccatta
      961 aaaaggcgtt ccaaattgcc gcaagcggcc gaccggggcc cgtggtggtt gatgtcccga
     1021 aagatgttac gcaggcgatg gcgaaattca gctatcctca ggaagacatt tttatccgtt
     1081 cgtatcaacc ggttgttcaa gggcatatcg gtcagattaa aaaggccgtg cagatgttgg
     1141 catctgccaa acgtccggtc gtctattttg gcggcggcgt ggtgttgggt aatgcttctg
     1201 aagagctgac ccgatttgtc cgaatgacgg gtgctccgtg cacgggtacg ctgatgggct
     1261 tgggcgctta tccttccggc gaccgccaat tcctcggtat gctcggtatg cacggtactt
     1321 acgaggcaaa ccttgccatg cagaatgcgg atgtcgttct tgccgtaggt gcgcgttttg
     1381 acgaccgtgt ggtttccgta ccgtccaaat ttttcgagaa ggcgaagaag gttatccata
     1441 tcgatgtcga tccttccagc atcgccaaac gcgtgaaggt ggacattccg attgtcggcg
     1501 acgtgaaaaa tgttttgtct gagatggttg cgctgtggca aaaacaagag ttcatgccgt
     1561 ctgaagatgc tttgggcaaa tggtggaaaa ccatagagga atggcgttcc caagattgct
     1621 tgtggtttga caacggcagc gaaattatca agccacaata tgtgattcag aagcttgccg
     1681 agattaccgg caattcggca atcatcacat cggatgtagg gcagcatcaa atgtttgcgg
     1741 ctcaatatta tcccttcgaa cgtccgcgcc aatggctcaa ttccggcggt ttgggtacga
     1801 tgggcgtggg tctgccttat gcgattggtg caaaacttgc cgccccggat caagacgtat
     1861 tctgtattac cggcgacggc tcgatccaga tgaacatcca agagttgtcc acctgtttcc
     1921 aatatcggat tccggttaac gtcattacgc tgaacaacgg ttatctcggt atggtacgcc
     1981 agtggcagga aatatattac ggcggccgag agtcggaaac ttatttcgat tctttgcccg
     2041 atttcgtcaa acttgccgag gcatacggcc atatcggtat ccgcgtggac aagaagtctg
     2101 atgtggaagg tgcgttgttg gaagcattga accaaaaaga caggctggtg tttatcgact
     2161 tcctgaccga ccagaaacag aatgtgatgc ccatggtcgg caacggcaaa ggtttggacg
     2221 aaatggtact tccgccgcat atgcgtgcgg acgaaaaggc gtaaggagag gcaaatgcga
     2281 catatcttat ctgttctgat tgaaaacgaa tcaggtgcga tgagccgcgt ggtcggtttg
     2341 ttctctgcac gcgattacaa tatcgattct ttggcggttg cgccgaccga agacaaaacc
     2401 ctgtcccgta tgaccatcgt tacccacggc gacgagcagg ttatcgaaca aatcaccaag
     2461 caactcaata aattgattga agtgattaaa gtggtcgatt tgaacgaaag ccgttttgtc
     2521 gaacgcgaac tgatgttggt aaaagtccgt gccgccggca aagaccgcga cgaatttttg
     2581 cgtttgaccg aaatctaccg gggcagcatc atcgacgtaa ccgaccgcag ctatacgatt
     2641 gaaattaccg gctcgaccga caagctggat tcctttttgg aaacggtcgg acgcgcccaa
     2701 attttggaaa ccgtacgtac aggcgcggcc ggcatcggtc gcggtgagcg tattttgaaa
     2761 atttaacgcc gtaacctttc agacggcacg gtatttgaat gccgtctgaa aaacgaacgg
     2821 caggagagat ttatgtcgaa cattaaaatt gtcgcactgg ttaccgtcaa accggaatac
     2881 acagaaacac tggtagcaca gtttaaagaa ctggtcaaag ccagccgtgc agaagagggc
     2941 aacatcagct acgatctcca tcaggaaatc ggtaaaccga accgttttgt tttcgtggaa
     3001 aattggaaat cccaagcagc tattgacgaa cataacgcca gcgcgcattt ccaagccttc
     3061 gtccagtccg tcgacggcaa aaccgaagcg ttggaaatcg tattgatgaa tgaagttgcc
     3121 gcttaagcgg cacactctgt ttaacccgtc tgaagccgct caacatttta aggcttcgac
     3181 aaccatttac cttaaaggaa atcaaatgca agtctattac gataaagatg ccgatctgtc
     3241 cctaatcaaa ggcaaaaccg ttgccatcat cggttacggt tcgcaaggtc atgcccatgc
     3301 tgccaacctg aaagattcgg gtgtaaacgt ggtgatcggt ctgcgccaag gttcttcttg
     3361 gaaaaaagcc gaagcagccg gccatgtcgt caaaaccgtt gctgaagcga ccaaagaagc
     3421 cgatgtcgtt atgctgctgc tgcctgacga aaccatgcct gccgtctatc acgccgaagt
     3481 tacagccaat ttgaaagaag gcgcaacgct ggcatttgca cacggcttca acgtgcacta
     3541 caaccaaatc gttccgcgtg ccgacttgga cgtgattatg gttgccccca aaggtccggg
     3601 tcataccgta cgcagtgaat acaaacgcgg cggcggcgtg ccttctctga ttgccgttta
     3661 ccaagacaat tccggcaaag ccaaagacat cgccctgtct tatgcggctg ccaacggcgg
     3721 caccaaaggc ggtgtgattg aaaccacttt ccgcgaagaa accgaaaccg atctgtttgg
     3781 cgaacaagcc gtattgtgcg gcggcgtggt cgagttgatc aaggcgggtt ttgaaaccct
     3841 gaccgaagcc ggttacgcgc ctgaaatggc ttacttcgaa tgtctgcacg aaatgaaact
     3901 gattgttgac ctgattttcg aaggcggtat tgccaatatg aactactcca tttccaacaa
     3961 tgcggagtac ggcgaatacg ttaccggccc tgaagtggtc aatgcttcca gcaaagaagc
     4021 catgcgcaat gccctgaaac gcattcaaac cggcgaatac gcaaaaatgt ttatccaaga
     4081 gggtaatgtc aactacgcgt ctatgactgc ccgccgccgt ctgaatgccg accaccaagt
     4141 tgaaaaagtc ggcgcacaac tgcgtgccat gatgccttgg attactgcca acaaattggt
     4201 tgaccaagac aaaaactgat tgttttcaaa cgggactgcc tacacatcgt gtaggcagtt
//
LOCUS       DAWEWP010000154         4234 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWEWP010000154 DAWEWP010000000
VERSION     DAWEWP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.154"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            13..1326
                     /locus_tag="WGN53_001754"
     CDS             13..1326
                     /locus_tag="WGN53_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ0140716.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHQHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            1409..2128
                     /locus_tag="WGN53_001755"
     CDS             1409..2128
                     /locus_tag="WGN53_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0140717.1"
                     /translation="MATLSDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            2293..2964
                     /locus_tag="WGN53_001756"
     CDS             2293..2964
                     /locus_tag="WGN53_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ0140718.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            2974..3342
                     /locus_tag="WGN53_001757"
     CDS             2974..3342
                     /locus_tag="WGN53_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ0140719.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            3339..3986
                     /locus_tag="WGN53_001758"
     CDS             3339..3986
                     /locus_tag="WGN53_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ0140720.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
BASE COUNT          996 a         1300 c         1109 g          829 t
ORIGIN      
        1 ttggaggaat aaatgatgaa cacgccgcat ccgcgcccga aaatcgccgt catcggcgca
       61 ggctgggcag gactgtccgc cgccgtcacc ttggcgcggc acgccgacgt taccctgttt
      121 gaagccggcc ggcaggcggg cggcagggcg cgcacactgg ccggaaatac cgacggtttc
      181 ggttttttgg acaacgggca gcacattttg ctcggcgcat accggggcgt gttgcgcctg
      241 atgaaaacca tcggttcgga tccccgtgcc gcctttttgc gcgtaccgct gcactggcat
      301 atgcacggcg gtttgcagtt ccgcgccctc cccctgcccg cgccgctgca tattttgggc
      361 ggcgtgctgc ttgcccggcg tgcaccgact gcattcaaag ccaaactgct tgccgatatg
      421 tccgatttgc agaagtccgc acgcctcgga cagcccgaca cgacagtggc gcaatggctg
      481 aaacagcgga acgtgccgcg tgccgccgtg atgcagtttt ggcagccctt ggtttggggc
      541 gcgctcaaca cgcctttgga aaccgcaagc ctgcgcgtgt tgtgcaacgt tttgtccgac
      601 ggcgtgctga cgaaaaaatc cggcagcgac tatctcctac ccaagcagga tttgggcgca
      661 atcgtcgccg aacccgcctt ggcggagctt caacggctcg gcgcggacat ccgcctcgaa
      721 acgcgcgtat gccgtctgaa caccctcccg gacgggaaag tgctcgtcaa cggcgaagct
      781 ttcgatgccg ccgtccccgc caccgcgccc taccacgccg ccgcgctcct gcccgaaggc
      841 acgcccgaac acgttcagac tgcatatcaa aaccttcgct accacgccat caccaccgtc
      901 tatctgcgct acgccgaacc cgtccgcctg cccgccccgc tgaccggcct tgccgacggc
      961 acggtgcaat ggctgctttg ccggggcagg ctcggactgc ctgaaaacga agtgtccgcc
     1021 gtcatcagcg tttccgaccg cgtcggcgcg tttgcaaacc gggcgtgggc ggacaaagtt
     1081 cacgccgacc tcaaacgcat ccttccgcat ttgggcgaac ccgaagccgt gcgcgtcatc
     1141 accgaaaaac gcgccacaac cgcagccgat gccccgccgc cggatttgtc gtggttgcac
     1201 cagcaccgca tcttccccgc cggcgactac ctccacccag actaccccgc cacgctcgaa
     1261 gccgccgtac aatcaggttt cgcgtcggcg gaagcctgcc tgcaaagcct gagcgatgcc
     1321 gtctgaaaac gccggccggt atcgggacgc tttacaaggt gcggcaaaac gctaaaatac
     1381 cttaaccgac caccaaaaag gaaaaagtat ggcgacactg tccgacaaaa ccatcttagt
     1441 taccggcgca tcgcaaggtc tgggcgaaca ggtcgccaaa gcctatgcgg cggcaggcgc
     1501 aaccgtgatt ctggtggcac gccatcagaa aaaactggaa aaagtctatg acgcgattgt
     1561 cgaagccgga taccccgaac cattcgccat ctgctttgac cttattagcg cggaagaaaa
     1621 agaatttgaa catttcgccg ccaccattgc cgaagccacg caaggcaaac tggacggcat
     1681 cgtccactgc gccggctatt tttacgccct ctcgccgctg gatttccaaa ccgtcgccga
     1741 atgggtcaac caataccgca tcaacaccgt cgcacctatg gggctgaccc gcgccctgtt
     1801 cccgctgctg aagcagtcgc ccgacgcgtc cgtcatcttc gtcggcgaaa gccacggcga
     1861 aacacccaaa gcctactggg gcggcttcgg cgcgtccaaa gccgcgttga actacctgtg
     1921 caaagtcgcc gccgacgaat gggaacgctt cggcaacctg cgcgccaacg tcctcgtccc
     1981 cggacccatc aattccccgc aacgcatcaa atcccatccg ggcgaagcca aaagcgaacg
     2041 caaaagctac ggggacgtgc tgcccgcatt tgtctggtgg gcaagtaccg aaagcaaagg
     2101 gcggagcggc gaaatcgttt acctctaaat ccggaaaagg caggcatccc gcccgcgccg
     2161 cgattccgtt tgaaaaatat ccgtaccttc cgcgcgcatc cgtcccgcac agacaaacac
     2221 cttgacttaa atttacataa ggacaataat gataacccgt tatttcctta acttgacctt
     2281 agggtaaatt ttatgaaaac cgtttccacc gccgttgtcc ttgccgccgc tgccgtttca
     2341 ctgaccggct gtgcgaccga atcctcacgc agtctcgagg tagagaaagt cgcctcctac
     2401 aatacgcaat atcacggtgt tcgtaccccg atttccgtcg gaacattcga caaccgctcc
     2461 agcttccaaa aaggcatttt ctccgacggg gaagaccgtt tgggcagcca ggcaaaaacc
     2521 attctagtaa cgcacctgca acagaccaac cgcttcaacg tactgaaccg caccaatttg
     2581 aacgcattaa aacaggaatc cggcatttcc ggcaaagcgc ataacctgaa aggcgcagat
     2641 tatgtcgtta ccggcgatgt aaccgaattc ggacgcagag atgtcggcga tcatcagctc
     2701 ttcggcattt tgggtcgcgg caaatcgcaa atcgcctatg caaaagtggc tctgaatatc
     2761 gtcaacgtca atacttccga aatcgtctat tccgcacagg gcgcgggcga atacgcactt
     2821 tccaaccgtg aaatcatcgg tttcggcggc acttccggct acgatgcgac tttgaacggc
     2881 aaagttttag acttggcaat ccgcgaagcc gtcaacagcc tggttcaggc tgttgacaac
     2941 ggcgcatggc aacccaaccg ttaaggagaa atgatgaatc cgaaaacctt gagccgtttg
     3001 tcgctgtgtg cggcggtctt ggctctgacc gcctgcggcg gcaacgggca aaaatccctg
     3061 tattattacg gcggctatcc cgataccgtc tatgaaggtt tgaaaaacga cgacacttcg
     3121 ttgggcaagc agaccgaaaa gatggaaaaa tactttgtgg aagccggcaa caaaaaaatg
     3181 aatgccgccc cgggtgcgca cgcccatctg ggactgctgc tttcccgttc gggagacaaa
     3241 gagggcgcgt tccgccagtt tgaagaagag aaaaggctgt ttcccgaatc gggcgtattt
     3301 atggacttcc tgatgaaaac aggtaaagga ggcaagcgat gaaacctttg attttagggc
     3361 ttgccgccgt gttggcgctg tctgcctgcc aagttcaaaa agcgcccgat ttcgactaca
     3421 cgtcattcaa ggaaagcaaa ccggcttcaa ttttggtggt tccgcctctg aacgaatcgc
     3481 ccgatgtcaa cggaacatgg ggtgtactgg cttcgaccgc cgcgccgctt tccgaagccg
     3541 gctattacgt cttccccgcc gcagtcgtgg aggaaacctt caaacaaaac ggcttgacca
     3601 atgccgccga tattcacgcc gtccggccgg aaaaactgca tcagattttc ggcaatgatg
     3661 cggttttgta cattacggtt accgaatacg gcacttcata tcaaatttta gacagcgtga
     3721 cgaccgtatc cgccaaagca cggctggtcg attcccgcaa cggaaaagag ttgtggtcgg
     3781 gttcggccag catccgcgaa ggcagcaaca acagcaacag cggcctgttg ggggctttgg
     3841 tcagcgcagt ggtcaatcag attgccaaca gcctgaccga ccgcggttat caggtttcca
     3901 aaaccgccgc atacaacctg ctgtcgccct attctcacaa cggcatcttg aaaggtccga
     3961 gattcgtcga agagcagccc aaataagcat atgccgtctg aacctctccg agcttcagac
     4021 ggcatatgcg aatcttgata cttgcaagcc gcaccgccca aaatcgagcg gccggtttca
     4081 atatccgaac aaggttcggg ttctgtattt tgcagaaccc gaacctttta ttcaaattta
     4141 aacttaattc cggcgtggtt gtatggtata gtggattaac aaaaatcagg acaaggcgac
     4201 gaagccgcag acagtacaaa tagtacggaa ccga
//
LOCUS       DAWEWP010000155         4214 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWEWP010000155 DAWEWP010000000
VERSION     DAWEWP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.155"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(83..1129)
                     /gene="recA"
                     /locus_tag="WGN53_001759"
     CDS             complement(83..1129)
                     /gene="recA"
                     /locus_tag="WGN53_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ0140721.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGIEMHIT
                     EGTQDETDGERPEE"
     gene            1386..2150
                     /locus_tag="WGN53_001760"
     CDS             1386..2150
                     /locus_tag="WGN53_001760"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ0140722.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2170..4185
                     /gene="rep"
                     /locus_tag="WGN53_001761"
     CDS             2170..4185
                     /gene="rep"
                     /locus_tag="WGN53_001761"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ0140723.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVSDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGNPSD"
BASE COUNT          999 a         1154 c         1155 g          906 t
ORIGIN      
        1 aaactcaaat caagccgttc ggaggctgct caaaaaaaac ggtacttcgc aacagaagta
       61 ccgtttatcg ggatttcagg ttttattctt cggggcgttc gccgtcggtt tcgtcctgcg
      121 tcccttcggt gatgtgcatt tctatgccgt tgagggcgcg gatttttgcg tcgatttcgt
      181 cggcaatttc gggattctcc ttcagccaga cgcggacgtt gtctttgccc tgaccgattt
      241 tcgcgccgtt gtagctgtac cacgcgccgg atttgttgat gatgtcgttt ttcacgccga
      301 tgtcgatcaa ttcgccttcc caactgatgc cttctccgta gaggatgtca aactctgcct
      361 gacggaacgg cggcgcgact ttgtttttga tgactttgac gcgggtttcg ttgcccaata
      421 cttcttcacc ttttttgatg gatccggtgc ggcggatgtc gaggcggacg gaagaataga
      481 atttcagcgc gttgccgccg gtcgtggtct cggggctgcc gaacattacg ccgatcttca
      541 tccggatttg gttgatgaac acaaccagcg tgttggtttt tttgatgtgt ccggtcagtt
      601 tgcgcaaagc ctggctcatc aggcgcgcct gcagtccgac atggctgtcc cccatatcgc
      661 cttcgatttc ggctttgggg acgagtgcgg ctacggaatc gacgactacc atatctatgc
      721 cgcccgaacg gacgagtgtg tcgcagattt ccaaagcctg ttcgccggta tcgggctggg
      781 acaggtaaag ctcttcgact tttacgccga gtttgcgggc gtaaacggga tcgaaggcgt
      841 gttcggcatc gacaaaggcg cacacgccgc cgtttttctg gcattgggcg acggcttcga
      901 ggcagagggt ggttttgccg gaggattctg ggccgaagat ttcgacgatg cgcccgcgcg
      961 gcagaccgcc gactccgagg gcgaggtcta atccgagcga tccggtggaa atgacttcga
     1021 ggttttcttc ctgctggctg ccgtccattt tcatgatggc gcctttgccg aaactttttt
     1081 cgatttgcgc cagtgcggcg gcaagtgctt tgcttttgtc gtctgacatt ggggttactc
     1141 cggaacaaat gcggtatgtg ggatgcggcg caacacgggc tgcggcgcgg ggtgcgtatc
     1201 gttttcccga tgtgcgggct gtcggtaatg ccgctgcttc acggggctgc cattatcgca
     1261 tatttccttg cttgccgata tgcgccagga cgcggcggct tgtgccggaa cggtgtccgg
     1321 atgccgtcta aaaggcggcc ggctttgtta taatggcggc tgttttttct gtgtgtgcct
     1381 gttttatgtg ttcctgcctt gttgtcaaaa ataccgttat cggaagcgga cgcaccaaaa
     1441 tcgccgtgcc gcttgtcgcc cgcgatgccg ccgaactttc cgccgtactt gagcaaatca
     1501 aaaatatgcc cttcgatatt gcggagttcc gcgccgactt tttggaatgc gcgggcagta
     1561 tcggcgaaat attgcaccac acgcagaccg tccgcgacgc gctgcccgac aagccgctgc
     1621 tgtttacgtt cagacggcat ggcgaaggcg gctcgttccc gtgttcggac gattattatt
     1681 ttaaactgct cgacgcgctg atcgaaagtc gcctgcccga cattatcgat atcgagctgt
     1741 tttccggcga aaccgccgtc cggcgcgctg tggcgaatgc tcaaaaaaac ggcatcgccg
     1801 ccctgctctg caatcatgag tttcaccgca cgccgccgca agaagaaatc gtatgccgtc
     1861 tgaaacagat ggaggactgc ggcgcggaca tctgcaaaat tgcggtgatg ccgcaaagcg
     1921 cggaagatgt gctgactttg ctttccgcca cgctcaaagc gaaagagctt gccgccaaac
     1981 cgattgttac gatgtcgatg gggcagacgg gggcggtcag ccggcttgcc ggacaggtgt
     2041 tcggctcaag catcacgttc ggttcgggaa cgcaaaactc cgcgccgggg caaatcggcg
     2101 tatccgccct ccgtgcgaca ctcgactgcc tcgaaaacgg cgcagactga tttcagacag
     2161 catcaaaaca tgatgaaact caatccccaa cagctcgaag ccgtccgcta cctcggcggc
     2221 ccactgctcg tccttgccgg tgcaggcagc ggcaaaaccg gcgtgattac tcaaaaaatc
     2281 aagtatttga ttgtcaatgt cggctatctg ccgcataccg ttgccgcgat tacctttacc
     2341 aataaagccg ccgcggaaat gcaggagcgc gtcgccaaaa tgctgcacaa atcgcaaacg
     2401 cgcgggctga cgatttgcac gttccactct ttgggcatga agattctgcg cgaagaggcg
     2461 aaccatatcg gttacaaaaa aaacttctcc attctcgatt ctaccgacag cgcgaaaatc
     2521 atcggcgaac tcttaggcgg tacgggcaaa gaagccgtat tcaaggcgca gcaccagatt
     2581 tccttgtgga aaaacgattt aaaaacgcct gaagatgtcg ttcagacggc atcgaacatt
     2641 tgggaacaac aaaccgcacg cgtgtatgcg agctatcagg aaaccttaca aagctatcag
     2701 gcagtggact tcgacgactt gatccgcctg cctgccgtgc tgttgcagca aaacagcgaa
     2761 gtgcgcaaca aatggcagcg gcggctgcgt tatctgctgg ttgacgaatg ccaagatacg
     2821 aatacctgcc aatttacgtt gatgaaactt ctgaccggcg cagaaggtat gtttaccgcc
     2881 gtcggcgacg acgaccagtc catctacgca tggcgcggcg cgaatatgga aaacctgcgc
     2941 aaaatgcagg aaaactatcc gcagatgaag gtcatcaaac tggagcaaaa ctaccgctcc
     3001 accgcgcgga ttctcaaaat cgccaacaaa gtcatcgaga acaaccccaa gctgttcacc
     3061 aaaaaacttt ggtcgaaatt gggcgaaggc gagcccgtca aagtcgttgc ctgccaaaac
     3121 gagcagcacg aagccgactg ggtcgtcagc caaatcgtca aacaaaaact catcggcggc
     3181 gacaaaaccc aatatgccga tttcgccgtg ttgtaccgcg gaaagcatca ggcgaggatt
     3241 ttcgaggaag cattgcgcgg cgcgcgcatc ccctaccgtc tctccggcgg acaaagcttt
     3301 ttcgacaaag ccgaaatcaa agacgtgttg tcttatgtgc ggctgcttgc caaccccaat
     3361 gacgatcccg cctttctgcg tgccgttacc acgcccaaac gcggcatcgg cgatgtcacg
     3421 ctgggcaagc tcaacactta cgcgcacgaa cacgaatgca gcctgtatga agccgcacaa
     3481 aacgaagaag cccttgccac gctgaacaat accaaccgcc aacacctgca agcctttatg
     3541 gatatgttcg gcagctaccg cgccaaagcg gaaaccggcg aagcaggcga gttcatcaac
     3601 agcctgctcg aagaaatcga ctatgaaaac catttgatgc aaaacgaaga aggcaaggcc
     3661 ggcgaaatca aatggcgcaa tgtcagcgat ttggtatcat ggtttgcgcg aaaaggcggg
     3721 gaagacggca aaaacatcat cgaactcgcc caaaccgtcg ccttgatgac gcttttggaa
     3781 ggaaaagacg aagaagaaac cgatgccgtc tcgctatcca cgctacacgc cgccaaaggt
     3841 ttggagtatc cgtatgtttt ccttgtcggt tgcgaagaag gcgttttgcc gcacaacgac
     3901 agtatcgaag agggcaacgt cgaagaagaa cgccgcctga tgtacgtcgg catcacccgc
     3961 gccaaacgcc aactcacgct gacccactgc gtcaaacgca aaaaacaagg cacatggcag
     4021 ttccccgaac ccagccgatt catagacgaa atgccgcagg aagatttgaa aatcctgggg
     4081 cgcaaaggcg gcgagccgat tgtcagcaag gaagaaggca gacgcaacct tgccgatata
     4141 atcggaaggc tcgacagcat gaaaaaaggc aatccgtcgg attaaaccaa gatagggaaa
     4201 cgaagccgga gaca
//
LOCUS       DAWEWP010000156         4192 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWEWP010000156 DAWEWP010000000
VERSION     DAWEWP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.156"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(79..315)
                     /locus_tag="WGN53_001762"
                     /pseudo
     CDS             complement(79..315)
                     /locus_tag="WGN53_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
     gene            511..969
                     /locus_tag="WGN53_001763"
                     /pseudo
     CDS             511..969
                     /locus_tag="WGN53_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            869..1237
                     /locus_tag="WGN53_001764"
     CDS             869..1237
                     /locus_tag="WGN53_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ0140724.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1306..2844
                     /gene="lnt"
                     /locus_tag="WGN53_001765"
     CDS             1306..2844
                     /gene="lnt"
                     /locus_tag="WGN53_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ0140725.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3108..3971
                     /gene="rpoH"
                     /locus_tag="WGN53_001766"
     CDS             3108..3971
                     /gene="rpoH"
                     /locus_tag="WGN53_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ0140726.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
BASE COUNT         1020 a         1295 c          967 g          910 t
ORIGIN      
        1 cgaccttgcc gtcgggcagg atgtaccacg ccaaatcccc gcctgaagca cggtgtcgga
       61 ctgcatttcg attcggctat acgccttttc acgcaggctg taattgaatt tccacaaacc
      121 gccgaacact gccggcggac gcgcgaccag catcaggaac gccgcaatcg ccaaggcttc
      181 cgccgccccg tccaacacgc cggcgggaga aaccagcagc aaaggcattc ccaaacgtgc
      241 gggtaaagtg gtcgaaacca cgctcagaaa caacagtgcg ccacccggca ggaacagact
      301 gcccatccaa tccacgctaa cgattctccc ttaaaattca ttaaattaaa atcgaaagac
      361 tgtaacacgg cattttcttt tcacgaaact ttacttcacg tccgccacag cgttcagacg
      421 gcattttcct gtttcatgcc gcctgaaacc gcccaaatcc gcgttctcct atataatgtc
      481 ggactttgcg ccgcatttca ggattcttta atggcatttg cctcactatt taccctgccg
      541 gacgacatca ccgccgtttt ggacgatgtt gccctgatga ccaaagccgc cgcaaagaaa
      601 accgccggcg tggtcggcga cgatttggca cccaatgcca accgtgttac gggcgtatcg
      661 gcagaacgcg agttgccgat tgtttggtcg atggcgaaag gttctttttc caacaagccg
      721 gttttggtgc ctgcggccct gctgctttcc gccttcctgc cgcaactcat tacgccgctt
      781 ttgacggcag gcggcattta tctgtgcttt gaaggcgtag aaaagctgct gcacaagttt
      841 ctgcaccgac atgacgcgca tgctcgtaat ggcggcaatc ggcatcggca tgactgtttt
      901 ggtgtacgga atcgttgccg tcatcgtcaa actcgacgac ttaggcatgc tgctgatgcg
      961 ccgcccacaa accttttcgc gcagtttggg gcaaatgctg actgccttta tgccttgctt
     1021 tatgcgcggt ttgagcgtgg tcggcacgct tgccatgttt cttatcggcg gcgtactggt
     1081 cgcccacaac ctcggccttc tgcacgattt cctgcacgcg caacactggg acgctggctg
     1141 ggcggagtac ttcgccaacc tcgttgtcgg gctgctttcc ggttcgattg cctgcgcccc
     1201 cgccttgccg ctgatgaatc gtttcggcag gcattgattt ctttttcaaa taccgatgcc
     1261 gtttgaaaga tgttcagacg gtatcttccg aacagacaga tgaatatggt ttccaaactg
     1321 gacaaatact ggcagcaccc cgccctctac tggcctttgc tcatcctttt tgccgccgcc
     1381 acccccttta ccttcgcacc ctactaccac ttttggctga tgcccttgat tttcggtgcc
     1441 ttcgtccgcc tcatcgaact gcgtccgcgt tttgctgtct cttccgccta cctgttcggc
     1501 ctgaccgcat acacgacaca gttctactgg atacacaccg ccctgcacga cgtttccggc
     1561 ctgcccgacc tctatgccgt accgctgacc ttcctactcc ccgcctacct tgccctttat
     1621 ccggcactgt gtttctggct gtggaaaaaa tttaccctgc ctcggggcat aaaaatcggt
     1681 ttggtactgc ccatcctgtg gacactgacc gagtttgccc gcgaacgttt cctgaccgga
     1741 ttcggctggg gcgcaatcgg ctactcccaa atcaccccgg acagcccgct cgccggcttt
     1801 gccccattgg gcggcatcca catggttaca ctggcaaccg cctttctcgg tgtctggctg
     1861 gttttggcga gtgataacac cgcacgttcg ggcaaacgcc tgcttccaat tatcctgatt
     1921 gccgccctgc ttgccgcagg ctacaccgcc cgacaaaccg acttcacccg ccccgacggc
     1981 agccgcagca ccgtcgccct gcttcaaggc aacatcgacc aaaccctcaa atggcgtgaa
     2041 gaccaagtca tcccgaccat acagaaatat tacgaacagg tcggcaaaac cactgccgac
     2101 atcgtcatcc tgcccgaaac cgccatcccc gttatgcgcc aaaacctgcc ggaaaacata
     2161 ctggcaaaat ttgccgaaca ggcgcaaaac aacggcagcg cgctcgccgt cggcatcagc
     2221 caatacactt cggacggcaa cggttacgaa aacgccgtca tcaacctgac cggttatcag
     2281 gaaaacaatc aggacggcat cccctactac gccaaaaacc acctcgtccc cttcggcgaa
     2341 tacaaaccgc tgcccttcct gaccacgccg ctttacaaaa tgatgaatat gcccctttcc
     2401 gacttccgca aaggcggcgg taagcaatcc gccctgctga tgaaaaacca aaaaatcgcc
     2461 ttcaacatct gttacgaaga cggattcggc gacgaactga ttgccgccgc caaagatgcc
     2521 acactgcttg ccaatgccag caatatggcg tggtacggaa aatccaacgc catgtaccag
     2581 cacctccaac aatcgcaggc gcgggctatg gaactcggac gctatatggt ccgtgccacc
     2641 aacaccggcg caaccgccat catctccccc aaaggcaaca tcatcgccca agcccaaccc
     2701 gatacggaaa ccgtattgga aggacacatc aaaggctatg tcggcgaaac gccctatatg
     2761 aaaaccggca gttcatggtg gttgatgggc atattgaccc tagccgcact gattcttttc
     2821 atcttccgaa acaaagaaca ctgatacctg cacagcccgt ccggacgggc tgtctttttt
     2881 caaaacggct atttacatac aattacgtat gtaatataat tctgaaatcg aattgagcca
     2941 tcaggtttac aaaatcaatc aaacctgcct tacaaaattt tcctgtcttt caaaccttag
     3001 cgcatatctc ctgccaaaca tacaaaatac aagccaagca gtgatatact gcatcccaac
     3061 acagcaaacc gccgatacgc agtttcagcc gaaaggaaat gccccaaatg aataacgctt
     3121 tcgcattacc cgccatccaa agcggcaacg gcagcctcga acaatacatc cacaccgtca
     3181 acagcatccc gatgctgtcc caggaagaag aaacccgcct cgccgaacgc cggataaagg
     3241 gcgacctcaa cgccgccaaa caactcatcc tgtcccacct gcgcgtcgtc gtttccatcg
     3301 cgcgcggcta tgacggctac gggctgaatc aggcagacct gattcaggaa ggcaatatcg
     3361 gactgatgaa ggcggtcaaa cgctacgagc ccggtagagg cgcgcgtctg ttttcatttg
     3421 ccgtacactg gattaaagcc gaaatccacg agtttatttt gagaaactgg cgtttggttc
     3481 gcgttgccac caccaaaccg caacgcaagc tgtttttcaa tttacgcagt atgcgtaaaa
     3541 acctcaacgc cctgtctccc aaagaggcac aagacatcgc cgacgatttg ggtgtcaaac
     3601 tttccgaagt tctggaaatg gaacagcgca tgacgggaca cgacatcgcc atcatggcag
     3661 acaacagcga cgacgaggac agtttcgccc ccatcgactg gcttgccgac cacgattccg
     3721 aaccgagccg gcaactatcg aaacaggcgc attacgccct gcaaacagaa ggtttacaaa
     3781 acgctttggc gcaactggat gacaggagcc gccgtattgt ggaaagccgc tggcttcaag
     3841 acgacggcgg gctgacgctg caccagcttg ctgcagaata cggcgtctct gccgagcgca
     3901 tccgccagat tgaagcaaaa gccatgcaga aactgcgtgg tttcctgacc gaagaagccg
     3961 aagcggtttg aaatatacgc aataaaatac ccgacaaatt gttttgtcgg gtattttatt
     4021 tggtaaacat caatgcggtt tgtgcgtcgt gctgtacacg attttatccg tccacgccgt
     4081 tgcaattgcc gaaatcaaaa agcagacatg caccagacac tgccgcatca gctgcttttc
     4141 cgacagattg gcggaattga tatagtggat taacaaaaat caggacaagg cg
//
LOCUS       DAWEWP010000157         4128 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWEWP010000157 DAWEWP010000000
VERSION     DAWEWP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.157"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            43..675
                     /locus_tag="WGN53_001767"
     CDS             43..675
                     /locus_tag="WGN53_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ0140727.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="WGN53_001768"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="WGN53_001768"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ0140728.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAGTLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCHNFVEIGLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPVSRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKVFIGSLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2198..4066
                     /locus_tag="WGN53_001769"
     CDS             2198..4066
                     /locus_tag="WGN53_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0140729.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSRFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTASGKRQLLSLLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDTTLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRGGALQ"
BASE COUNT          935 a         1113 c         1073 g         1007 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcgggc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 acggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttcagag tacggcctga
      841 tgaaggcgcg cgtcaaagtc gaattaaact ggctcaaagc cctggccgcc gagccgaaga
      901 ttgtcgaagt gccgcccttc agtgccggaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg ctgaaaaaac gttttgccga agtgccggaa gtcgccgccg
     1081 tgagtgagtt catccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa gctggccgaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggcc
     1261 agcccgccac gcccacaaca ctgggcaaag aaaccgccaa cgtcgtgtac cgcctgcaac
     1321 gccagttcaa aatccttcag gcgcaagaat tcctcggcaa aatcaacggt gcggtcggca
     1381 actacaatgc ccatatggcc gcttatcccg atgtggattg ggaaacccac tgccacaact
     1441 tcgttgaaat cggcctcggt ttgaccttca acccctatac catccaaatc gagccgcacg
     1501 actatatggc tgaattcttc caaaccctca gccgcatcaa cacgattctc atcgacttta
     1561 accgcgacgt ttggggttat atttcattgg gttacttcaa acaaaaagtc aaagcgggcg
     1621 aagtcggctc ttccacgatg ccgcacaaag tcaaccccat cgattttgaa aactccgaag
     1681 gcaacctcgg tatggcaaac gccgtattgg gttttttggc ggaaaaactg cccgtctcac
     1741 gctggcagcg cgatttgacc gacagcaccg tcttacgcaa tatgggcgtg ggcgtgggct
     1801 atgccgtgtt gggctttgcc gcccacctgc gcggtctgaa caagctcgaa cccaaccctg
     1861 ccgcgcttgc cgccgatttg gatgccactt gggagctgct cgccgagccg attcaaaccg
     1921 tgatgcgtcg ttacggcgtg gccaatcctt acgaaaagct caaagacctg acgcgcggca
     1981 aaggcggcat tacgcccgaa gtgctgaaag tcttcatcgg atcgttggaa attccggcag
     2041 aagccaaagc aaaattgctt gagctgactc ctgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacgggggc gatatttatg caagctgtcc gatacaggcc
     2221 tgaaattgac ggattgcggg ccgttgccgt gctatccgtc attattttcc acctgaataa
     2281 ccgctggctg cccggagggt ttttgggggt ggacattttc tttgtcatct cgggattcct
     2341 cattaccggc atcattcttt ctgaaataca gaacggttct ttttctttcc gggattttta
     2401 tacccgcagg attaagcgga tttatcctgc ttttatcgcg gccgtgtcgc tggcttcggt
     2461 gattgcctct caaatcttcc tttacgaaga tttcaaccaa atgcggaaaa ccgtggagct
     2521 ttctgcggtt ttcttgtcca atatttatct ggggtttcag caggggtatt tcgatttgag
     2581 tgccgacgag aaccccgtac tgcatatctg gtctttggcg gtagaggaac agtattacct
     2641 cctgtatcct cttttgctga tattttgctg caaaaaaacc aaatcgctac gggtgctgcg
     2701 taacatcagc atcatcctat ttctgatttt gactgccaca tcgtttttgc caagcaggtt
     2761 ttataccgac atcctcaacc aacccaatac ttattacctt tcgacactga ggtttcccga
     2821 gctgttggca ggttcgctgc tggcggttta cgggcaaacg caaaacggca gacggcaaac
     2881 agcaagcgga aaacggcagt tgctttcatt actctgcttc ggcgcattgc ttgcctgcct
     2941 gttcgtgatt gacaaacaca atccgtttat cccgggaatg accctgctcc ttccctgcct
     3001 gctgacggca ctgcttatcc ggagtatgca atacgggaca cttccgaccc gcatcctgtc
     3061 ggcaagcccc atcgtatttg tcggcaaaat ctcttattcc ctatacctgt accattggat
     3121 ttttattgcc ttcgcccatt acattacagg cgacaaacag ctcggactgc ctgccgtatc
     3181 ggcggttgcc gcattgacgg ccggattttc cctgttgagc tattatttga ttgaacagcc
     3241 gcttagaaaa cggaagatga ccttcaaaaa ggcatttttc tgcctctatc tcgccccgtc
     3301 cctgatactt gtcggttaca acctgtacgc aagggggata ttgaaacagg aacacctccg
     3361 cccgttgccc ggcgcgcccc ttgctgcgga aaatcatttt ccggaaaccg tcctgaccct
     3421 cggcgactcg cacgccggac acctgcgggg ttttctggat tatgtcggca gccgggaagg
     3481 gtggaaagcc aaaatcctgt ccctcgattc ggagtgtttg gtttgggtgg atacgacact
     3541 ggcagacaac ccgttatgtc gaaaataccg ggatgaagtt gaaaaagccg aagccgtttt
     3601 cattgcccaa ttctatgatt tgaggatggg cggccagccc gtgccgagat ttgaagcgca
     3661 atccttccta atacccgggt tccaagcccg attcagggaa accgtcaaaa ggatagccgc
     3721 cgtcaaaccc gtctatgttt ttgcaaacaa cacatcaatc agccgttcgc ccctgaggga
     3781 ggaaaaattg aaaagatttg ccgcaaacca atatctccgc cccattcagg ctatgggcga
     3841 catcggcaag agcaatcagg cggtctttga tttggttaaa gatatcccca atgtgcattg
     3901 ggtggacgca cagaaatacc tgcctaaaaa cacggtcgaa atacacggac gctatcttta
     3961 cggcgaccaa gaccacctga cctatttcgg ttcttattat atggggcggg aatttcacaa
     4021 acacgaacgc ctgcttaaat cttcccgcgg cggcgcattg cagtagcctg ccttcttgtc
     4081 ggatattgcc tttggcagcc tatgccgctg tttgcccttc ggggcggc
//
LOCUS       DAWEWP010000158         4098 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWEWP010000158 DAWEWP010000000
VERSION     DAWEWP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.158"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(202..1098)
                     /gene="argB"
                     /locus_tag="WGN53_001770"
     CDS             complement(202..1098)
                     /gene="argB"
                     /locus_tag="WGN53_001770"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ0140730.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGYIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKMGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1268..2398
                     /locus_tag="WGN53_001771"
     CDS             1268..2398
                     /locus_tag="WGN53_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ0140731.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFDNAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDHQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGVNLNREWENPTVEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANVTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            2471..3322
                     /locus_tag="WGN53_001772"
     CDS             2471..3322
                     /locus_tag="WGN53_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ0140732.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3405..4067)
                     /locus_tag="WGN53_001773"
     CDS             complement(3405..4067)
                     /locus_tag="WGN53_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ0140733.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          904 a         1195 c          997 g         1002 t
ORIGIN      
        1 aaaatcaaag ccggagaatt tatcccaaac aaccggattt caaaaaaacc agattcccgc
       61 cttcgcggga atgacggatt ttaggtttct gttttggttt ctgttttcgc gggaataaca
      121 acttagaaat tgcccgaaac cccaaaaaca gaaaccggac aaacgggttt tccgccaaag
      181 ccggcatttt ccgactttgc ttcaggcatc ttccccgccg cctaaaatca tcgaaccgat
      241 accggcatca gtaaagattt ccaacaaaag cgcgttgggc aacctgccgt cgatgatgtg
      301 cgtggctttc acaccgttga cggcggcttc gaccgcagaa gcgattttcg gcagcatacc
      361 gccatacagc gtgccgtcgg caatcagttc atcaatccgt ttcggcgtga gtttggtcag
      421 cagattgccc attttgtcca tcacaccggc gatattcgtc atcatcaaga gtttttcagc
      481 gttcaattct tccgccaatt tgcctgctac caaatcggcg ttgatgttga acgcttcgcc
      541 tttttcacct acgccgacgg gggcgacgac gggaatgtag ccgcgttcta tcagcccttt
      601 aaccaaaccg gtatcgatgc tttccaccgt accgacctgt ccgatgtcca cgccattctg
      661 ttcgggcgta tcgatcaaaa gtttcttcgc cttaatgaaa tgatcgtcgc gtccgctcac
      721 gccgaccgcg tgtccgccat atgtgttaat catcgacacg atttctttat tgacatgccc
      781 gcccaacacc atttcgacaa tatccatcgc ctctttgtcg gtaacgcgca ttccttggac
      841 aaactcaccc tttttgccga ctttttcaag catcgcattg atctgcggcc cgccgccgtg
      901 aacgatgacg ggatgaatgc cgaccagctt cagcagcacg acatcgcggg caaacccttc
      961 tttcaaggca ggttcggtca tcgcgttgcc gccgtatttg atgacggcga ccgaaccgga
     1021 aaaccggcgg atgtaaggca gcgcttcggc aaggatacgc gccttgtcgg cggcggaaat
     1081 aatgttttca gactccataa cggctcccta ttgttgacaa tttcggctta tgttaacgcc
     1141 aaacgccgtc tgaaacaatc ttcagacggc atccggcaga ctttcgggca ggttgaagcc
     1201 ttgcgatacg ggcggtaaaa tcgcggttta tccggttcga gaaaccggcg acagcaagga
     1261 ctttcccatg attaaaatca gcacccgctt cgatgccggc tcggtcgtcg tcaaagacct
     1321 gaccgatcct tccaacatcc gcctcgccct gcgtcccgac aacgcctccg attttgcaca
     1381 atggttctac ttccggctgc aaggcgcggc atatcaaaac tgcatcatgc attttgacaa
     1441 tgcggcagaa gccgcctacc cgaaaggctg ggaaggctat caggcgtgtg cctcatacga
     1501 ccgccgcaac tggttccgcg taccgacttc ctacgaaaac ggcgtactga ccgtcaacca
     1561 taccccgctg tccaatagcg tgtactacgc ctatttcgaa ccctattccg aagaacagca
     1621 cctcaacctc ctcggcgacg cgcaaggcag cggcctgtgc cgcatcgacg acttgggcag
     1681 caccgtgcaa gggcgcgaca tcaatctgct gaccataggc aaccaggtcg aaagcgattt
     1741 gaaaatctgg attaccgccc gccagcatcc aggcgaaacc atggcggaat ggtttgtcga
     1801 ggggctgctc ggcagactgc tcgaccatca ggatgcaacc gcgcgcaccc tgctcgatcg
     1861 cgccacgttc tatatcgtcc ccaacatgaa ccccgacggt tcggcactgg gcaaccttcg
     1921 caccaacgcc gccggtgtga acctcaaccg cgaatgggaa aatccgactg tggaaaaaag
     1981 ccccgaagtg ttcttcgtgc gtgaaagaat gctggaaacc ggcgtggatt tgtttttgga
     2041 tatccacggc gacgaagggt tgccctttgt ctttgttgca ggaactgaag gcgtgccgaa
     2101 ctacaatccg cgcatttccg cgttggaaac acagttcaaa accgcccttt tgaacgccag
     2161 ccccgatttc caagatgaat acggttacga aaaagatgcg cccggtcagg caaacgtgac
     2221 cttggcaaca aactgggtcg gcaaccgttt caactgcctt gcctatacgt tggaaatgcc
     2281 gttcaaagac aatgccaacc tgcccgacga cgacttcggg tggaacggtc aacgttcgtt
     2341 gcgcttgggc gaagcggtgc tttccgccat tttgaacgtc gccggcgatt tgcgttgaca
     2401 ctccccactg aaaaatgccg tctgaaagcc cttccccttt tcagacggca tttgaaaacg
     2461 aaagcccgtt atgattaccc atccccaatt cgaccccgtc cttatcagta tcggtccgct
     2521 tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta cctttctcgg
     2581 cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgcttg acgacttcct
     2641 cacttggggc attttgggcg taatttttgg tgggcgtttg ggttacgtcc tgttttacaa
     2701 gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag gcggaatgtc
     2761 gttccacggc ggctttttgg gtgtagttat tgccatatgg ttgttcggcc gcaaacacgg
     2821 catcggcttc ctcaaactga tggacacggt cgcaccgctc gttccgctgg gtctcgcttc
     2881 gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg acatcaacgc
     2941 attttgggca atgggcttcc cgcaggcgcg ttacgaagat gccgaagccg ccgcgcacaa
     3001 tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc cctcgcagct
     3061 ttatcagttt gcacttgaag gcatctgcct gttcgccgtc gtttggctgt tctctaaaaa
     3121 acagcggccg accggacaag tcgcctcgct cttcctcggc ggctacggca tattccgctt
     3181 cattgccgaa ttcgcacgcc aacccgacga ctatctcggg ctgctgacct tggggctgtc
     3241 gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct ttgtccggtt
     3301 cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca tggaaatccg
     3361 gtttcagacg gcatttttac gtttggttca tggaaacaaa cgcttcaatt cttaccgcaa
     3421 cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc tgttttttcg
     3481 ccttccctgc ggacggtttg cggcttgttg atgaccgcct gccagtagcg gtagatatcc
     3541 gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt gaattgcaga
     3601 cggatagtgc cgttgtcctc ttcgtcgtaa ataccgccca atgccatgat gggataaaac
     3661 aactcttcaa acgcttcatc atcgacggct tcaaaccaat cggtcggcac aatatccaaa
     3721 ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc gttttcatac
     3781 agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat cgccattgcc
     3841 aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc gtcgcccaac
     3901 acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag cgccgtcata
     3961 aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc atccaacaat
     4021 tcgctcaacc gccgtttgga tgcttcggta aatttttggg aatccatcat tttccttttc
     4081 aaatgggttt tgcgccct
//
LOCUS       DAWEWP010000159         4096 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWEWP010000159 DAWEWP010000000
VERSION     DAWEWP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.159"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1013..1555
                     /locus_tag="WGN53_001774"
     CDS             1013..1555
                     /locus_tag="WGN53_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0140734.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
     gene            1685..3346
                     /locus_tag="WGN53_001775"
     CDS             1685..3346
                     /locus_tag="WGN53_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ0140735.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHKDGSVKGIATGNM
                     GVGKNGEPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGFQSLPKLSFKGGVLVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVENFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPESASGPNYGGM"
     gene            complement(3480..3974)
                     /locus_tag="WGN53_001776"
     CDS             complement(3480..3974)
                     /locus_tag="WGN53_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ0140736.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3971..>4096)
                     /locus_tag="WGN53_001777"
     CDS             complement(3971..>4096)
                     /locus_tag="WGN53_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0140737.1"
                     /translation="AGMAGLSGQISLTVPVLLTAQVLWVIIPLVLAAGIFRKRQI"
BASE COUNT         1030 a         1012 c         1093 g          961 t
ORIGIN      
        1 aatttcgatt taacttcggc acaccgtctc aacagcttaa aatcctgcaa aaatgatgtg
       61 gaatttaggg ctaatctagt acagcccctt agatttaatt cactatacct gcgtaatggt
      121 atcgggtact catcatcaac ctgccttgca ttcggttgtg ttgtccggca actgtccggt
      181 tgtgtcgatg ggttgccctg ccgctccctg tgctacgcaa cggttgtgtg ccttgggtgg
      241 gcgcgggtgg cggccgggcg gtttgttgct atgatacggg agttccgata tacaagggtg
      301 ggcttcagcc caccaatcaa cgaccgaaaa agaaaaaacg ttgggctgaa gcccactcta
      361 cgcaattacg caactgttat cgtggggatg acgggatttt aggtttttgt ttttctgttt
      421 ttgcgggaag gatgggtttt aggttgcggt catttatcgg aaaagcagaa atcgctccgc
      481 cgtcattccc gcgaaagcgg gaatctgaac acgtccgtag ggaaacctat atcccgtcat
      541 tcccacgaaa gtgggaatct agaacctctg aattttcaga caacctttga atattgccgc
      601 cgtcccgcgt tctagattcc cgcctgcgcg ggaatgacga ttcatcagtt tcccgaaacg
      661 cccgaaaaac cgaaaccgag caaaccggat tcccgcctgc gcgggaatga cgatttataa
      721 gtttcccgaa acgcccaaaa aaaccgaaat ccgacaggcg ggatttttgc tttcgtgggg
      781 atgacgggat tttaagttac ggtcatttat cggaaaagta gaaatcgctc cgccgtcatt
      841 ccagcgaagg cgggaatctg aacacgtccg cacggaaacc tatagtggat taacaaaaat
      901 caggacaagg cgacgaaacc gcagacagta cagatagtac ggcaaggcga ggtaacgccg
      961 tactggttta aatttaatcc actataatcc tgttgcttat ggaaaaatca aagtgcagag
     1021 ttggaaagag aggcgggatt tcaatattgt aaagcaggat ttggattttt cctgtggggc
     1081 ggcttcggtg gcgacgcttt tgaacaattt ttacgggcaa aagctgacgg aagaggacgt
     1141 gttaaaaaaa ctggataagg agcagatgcc cgcgtcgttt gaggatatgc ggcgcattat
     1201 gcctgatttg ggttttgaag cgaaaggcta tgccctgtct ttcgagcagc ttgcacagtt
     1261 gcaaatcccc gtcatcgtgt atctgaaata ccgcaaggat gatcatttct cggtattgcg
     1321 cggtatagac ggcaatacgg ttttgcttgc cgacccgtcg ctgggtcatg tttcaatgag
     1381 cagggcgcag tttttggatg cttggcaaac ccgtgaggga aatttggcag gtaagatttt
     1441 ggcggttgtg ccgaaaggga gggatgccgg cggggataag gcgtttttca cacgcagtcc
     1501 gaaacgtcag acggagtttg cggtcggaca ggtaaaatgg tggcgtgctt attgaaattt
     1561 cgacaaaggt cgtctgaaaa ccgaaaatat ggttttcaga cgacctttgt tgtatttggt
     1621 aactatatgt tctcgttgta taattacgga tttgcaattc aataataaat acacaggaac
     1681 cgccatgaca gaatccatca cccgcgacag tatgcaatac gatgtcgtga ttgtcggcgc
     1741 aggcccatcg ggtttgtccg ccgccatcaa actcaagcag cttgccgaac aaaatgggcg
     1801 cgaaatcagc gtttgcgtgg tggagaaagg ttcggaggcg ggtgcacatt cgctggcggg
     1861 cgcggttatc gatccgattg cgctgaatga gttgattccc gactggaaag aaaaaggcgc
     1921 gccgctgacg cgtacggtaa cgcaggacaa agtgttgttc ctgactgaga aaaaagcgtt
     1981 caatctgcct attaccccaa atttcgacaa tcacggcaac tacatcgtca gcttgggcga
     2041 agtcgtgcgc tggttggcgg agcaggcgga aaatttgggc gtggaaattt atccgggctt
     2101 tgccgctgcc gaagtgctgt atcacaaaga cggttcggtc aaaggcattg caaccggcaa
     2161 tatgggcgtg ggcaaaaacg gcgagccgac cgattcattc cagcccggca tggagctttg
     2221 ggcacggcaa accctgtttg ccgagggctg ccgaggttcg ctttccaaac aaatcatcga
     2281 acgcttcaaa ctcgaccaaa acagccagcc gcaaacttac ggcttgggca ttaaagaagt
     2341 ttgggaagtg ccgtccgaac agcatcagcc cggtttggtg gtgcacagcg caggctggcc
     2401 gctggacagc aaaacctacg gcggcgcgtt tgtttaccat ttcgacgaca acaaagttgc
     2461 cgtcggcttc gtggtcggtt tggactatca aaatccttat ctgtcgccgt ttgaagagtt
     2521 ccaacgtttc aaaacccatc ccgaaatccg caaaaccttt gaaggcggcc gccgcattgc
     2581 ttacggtgcg cgttcgctga ttgaaggcgg ttttcaaagt ttgccgaagc tctcgttcaa
     2641 aggcggcgtt ttggtcggcg atgcggcggg tttcctcaat atgccgcgca tcaaaggcat
     2701 tcataccgcg atgaaatccg ccatgctcgc cgccgaagcc gtgttcccct tgttggaaaa
     2761 ccttgaagaa gtggaaaact tcgacagcgg caaagaggcg gcggattatc agcaacgttt
     2821 tgaacaaagc tggctgtatc aagagcttta cgccgcgcgc aatgtccgtc cgtcattcaa
     2881 atggggcgtt tacctcggct caatctacac cggcatcgat cagatgattt tcagaggcaa
     2941 agccccgtgg accttgaaac atcacggcaa agacaacgag cagcttaaaa aagccgccgc
     3001 gtgcaagccg attgattatc cgaaacccga cggcgtattg accttcgacc gcttgagcag
     3061 cgtcttcctt gccaatctcg cgcacgaaga aaaccagccc gaccatttgg tgctgaacaa
     3121 tccgcaaacg atgatagacg tgaactacaa agaatacgcc tcacccgaaa cgcgctattg
     3181 tccggccggc gtgtatgaaa tcgtcgaaga aaacggcagc ccgcgcctgc aaatcaacgc
     3241 ggcaaactgc gtgcactgca aaacctgcga catcaaagac ccgacgcaaa acatcacttg
     3301 gatttgcccc gaaagcgcga gcgggccgaa ttacggcggg atgtaaacct tattccgccg
     3361 ttacgcccgg tcaggaataa ggaatggggc agttgttcaa acctgccccg tttgaaccta
     3421 tgacaaaaaa tgccgtctga accttggctt caacgttcag acggcatttt tgtttggatt
     3481 tacttgaaaa tataggtatc aggcatatcg tcgaaaccga caaccttacc gcctttatcc
     3541 tttgcaaatt tctcagcctg ctctttgttg ccgaacggca gcgcgtcttc cgcacccata
     3601 ccgccgataa agccgctgtc gatgacgtaa aaggcttttt tcgcatccat ccactccgtg
     3661 tcggcattgg gattcgtcca atcggtaaca ttgcccatat cggtaacgta aatcacgcgg
     3721 atgcctttgg gttcttcggg cagcttggta tagccgaaca tctgcttgat ggtggagaac
     3781 caaacgggct gatcgggttt gccgttcaag aaaatctggg ctttggggcc gttgtgttcg
     3841 gtcaggttca tactgcaata gtgtccgacc gaacggtcgc taatctgccg gggtaaaggc
     3901 ggcggcccct cttccgcctg ccggcaggca cttaaggcgg aaacggcaac aattgccaac
     3961 agggttttct tcatatttgt cgctttctaa aaattccggc tgccaaaaca agcggaatga
     4021 taacccataa aacctgagcg gtcagcaaaa cggggacggt caggctgatt tgtccgctca
     4081 aacccgccat acccgc
//
LOCUS       DAWEWP010000160         4061 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWEWP010000160 DAWEWP010000000
VERSION     DAWEWP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.160"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            39..462
                     /locus_tag="WGN53_001778"
                     /pseudo
     CDS             39..462
                     /locus_tag="WGN53_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            568..1131
                     /gene="pgsA"
                     /locus_tag="WGN53_001779"
     CDS             568..1131
                     /gene="pgsA"
                     /locus_tag="WGN53_001779"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ0140738.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1211..1286
                     /locus_tag="WGN53_001780"
     tRNA            1211..1286
                     /locus_tag="WGN53_001780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1244..1246,aa:Gly,seq:gcc)
     gene            1312..1387
                     /locus_tag="WGN53_001781"
     tRNA            1312..1387
                     /locus_tag="WGN53_001781"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1345..1347,aa:Gly,seq:gcc)
     gene            1411..1486
                     /locus_tag="WGN53_001782"
     tRNA            1411..1486
                     /locus_tag="WGN53_001782"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1444..1446,aa:Gly,seq:gcc)
     gene            1508..1583
                     /locus_tag="WGN53_001783"
     tRNA            1508..1583
                     /locus_tag="WGN53_001783"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1541..1543,aa:Gly,seq:gcc)
     gene            1609..1682
                     /locus_tag="WGN53_001784"
     tRNA            1609..1682
                     /locus_tag="WGN53_001784"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1641..1643,aa:Cys,seq:gca)
     gene            1785..1874
                     /locus_tag="WGN53_001785"
     tRNA            1785..1874
                     /locus_tag="WGN53_001785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1819..1821,aa:Leu,seq:taa)
     gene            1978..3030
                     /gene="sohB"
                     /locus_tag="WGN53_001786"
     CDS             1978..3030
                     /gene="sohB"
                     /locus_tag="WGN53_001786"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ0140739.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3081..3944)
                     /locus_tag="WGN53_001787"
     CDS             complement(3081..3944)
                     /locus_tag="WGN53_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ0140740.1"
                     /translation="MENVETHSQNHRKRLIGTFFLVGLENLLMLVYPVFGGWAINAVI
                     AGQAWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVT
                     ARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPR
                     FAAISENLYFRLKNSLERDNHFIRKGDERQLDRHYGLLARLRVLISNREAFGYLCVGT
                     AMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRI
                     EWSKRNIKAGT"
     gene            complement(3957..>4061)
                     /locus_tag="WGN53_001788"
     CDS             complement(3957..>4061)
                     /locus_tag="WGN53_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0140741.1"
                     /translation="KVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1025 a          965 c         1058 g         1013 t
ORIGIN      
        1 atcgctgggt atggtttgcc gccataagct gtctgctgtt gtccgccgtt tttattgcac
       61 cttacctgac ggcatttcac gaacaagaaa aaacattcga atatgcggaa ttgaccgtta
      121 ccgcccccaa ccgcagcgga cgggcaatca aactggatgc agacgggcag cattaccggc
      181 tttcctgcta tgggttcgac agtttgtgca caggcggcaa tatcggcaga accatcaggg
      241 cggagcaggt tagagccgtt ttaagcgaaa ccgtcggcaa aggtttttta aacggcgttc
      301 tgttggaata ccgcaacagt ggcagtgtct ataagcaata aagattttgc ccgcacggaa
      361 gaccgccttg tcgaagtgtt ggcacaacct gccgttttct gcctgaaact gggtattttg
      421 cttttattgc ccgccatttt cctgcgtttg aaaagaatgt gaaaacagat tggcggggcg
      481 ggggaatgcc catgcagcaa ttttacgttt tgggttatga ggataaccgt tataatcaca
      541 actttacagt ctgcacagag aaaaccgatg ccttggaatc tccctatctt cctcacatgg
      601 ttgagggtct tgctcattcc tgtcatgaca gccctttttt acctgccttt tccgtggttc
      661 gcggaggaaa cgattaatct caccgccgcc gtcatttttg ccgttgccgc cttaaccgac
      721 tggtttgacg gatttttggc aagactgtgg aaacagactt ccgatttcgg cgccttcctc
      781 gatcccgtcg ccgacaagct gatggttgcc gtatcgctac ttttactggt caaactcgac
      841 cggacctatg ttttgtttgc catgatcatt atcggcaggg aaattaccat ttccgcattg
      901 cgcgaatgga tggcgcaaat gggcaaaagg aacagcgttg ccgtcgccac cgtcggtaag
      961 ttcaaaaccg ccgcgcaaat gctggcaatt ttcctgctgc tgctgaatat tcccgatttt
     1021 tacggattta atcttgttgt tatcggcaat gtattgatgt ttatcgcatc tttgctgaca
     1081 gtctggtcga tgctgtatta tctgaaaatg gcgtggaaag aaatcgcctg aaaaaaacat
     1141 aaaaatagct tgacggcaaa aacagaatcc ataataattg cgtctcttcg atgttggaga
     1201 atgaaatcgg gcgggaatag ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc
     1261 gagttcgaga ctcgtttccc gctccaaaaa tttcattttc ccacaatcat tgcgggaata
     1321 gctcagttgg tagagcgcaa ccttgccaag gttgaggtcg cgagttcgag actcgtttcc
     1381 cgctccaagt tttatttcaa acatatcaac gcgggaatag ctcagttggt agagcgcaac
     1441 cttgccaagg ttgaggtcga gagttcgaga ctcgttttcc gctccaaaaa ccgatatgtt
     1501 tgaacgcgcg ggaatagctc agttggtaga gcgcaacctt gccaaggttg aggtcgcgag
     1561 ttcgagactc gtttcccgct ccaagttttt tagataagcc agttttaggg cgagatagca
     1621 aagtggttat gcagcggatt gcaaatccgt ctacgccggt tcgattccga ctctcgcctc
     1681 cattatcgta ctacggcggg gtggcagagt gtttatgcta tgaggagtgc aatcttcata
     1741 taggccggtt aaaatccgcg cccccgcctc cacctttcac aaatgcccgg gtggtgaaat
     1801 aggtagacac aacggactta aaatccgtcg ggactaaaca tcccgtgccg gttcgattcc
     1861 ggctccgggc accaagctga aaatgacaat gccgctccaa gcggttattt ttttatctgt
     1921 cggacggtga tggacaatat tgacatgttc atgcctgaac aagaggaaat ccaatcaatg
     1981 tggaaagaaa ttttactgaa ttacggtatt ttcctgctcg aactgcttac cgtgttcggc
     2041 gcaattgcgc tgattgtgtt ggctatcgta cagagtaaga aacagtcgga aagcggcagt
     2101 gtcgtactga cggatttttc ggaaaattat aaaaaacagc ggcaatcgtt tgaagcattc
     2161 tttttaagcg gggaagaggc aaaacatcag gaaaaagagg aaaagaaaaa ggaaaaggcg
     2221 gaagccaaag cagagaaaaa gcgtttgaag gagggtgggg agaaatcttc cgaaacgcaa
     2281 aaatcccgcc tttttgtgtt ggattttgac ggcgatttgt atgcacacgc cgtagaatcc
     2341 ttgcgtcatg agattacggc ggtgcttttg attgccaagc ctgaagatga ggttctgctt
     2401 agattggaaa gtccgggcgg cgtggttcac ggttacggtt tggcggcttc gcagcttagg
     2461 cgtttgcgcg aacgcaatat tccgctgacc gtcgccgtcg ataaggtggc ggcgagcggt
     2521 ggttatatga tggcgtgtgt ggcggataaa attgtttccg ctccgtttgc gattgtcggt
     2581 tcggtgggtg ttgtagcgga agtaccgaat atccaccgcc tgttgaaaaa acatgatatt
     2641 gatgtggatg tgatgacggc gggcgaattt aagcgcacgg ttacttttat gggtgaaaat
     2701 acggaaaagg gcaaacagaa attccgacag gaactggagg aaacgcatca gttgttcaag
     2761 cagtttgtca gcgagaaccg ccctcaattg gatattgagg aagtggcaac gggcgagcat
     2821 tggttcggtc ggcaggcgtt ggcgttgaac ttgattgacg agatttcgac cagtgatgat
     2881 ttgttgttga aagcgtttga aaacaaacag gttatcgaag tgaaatatca ggagaagcaa
     2941 agcctgatcc agcgcattgg tttgcaggcg gaagcttctg ttgaaaagtt gtttgccaaa
     3001 cttgtcaacc ggcgggcgga tgtgatgtag tttgccgaac ggatggaaat gccgtctgaa
     3061 gcgtgttcag acggcatttt tcaagttccg gctttgatgt tccgtttcga ccactctatc
     3121 cgttgtccga tgtctttcaa attggaatat tgttcgacca atcgcggcac gtcgtccaaa
     3181 ctcatggcaa acatccacag ataagtgccg accgaataga catgccccgc gctgctgtag
     3241 cctttgagcg tcatcatcac aaaagcaaag ccgaacaaaa tacccatcgc cgtgccgacg
     3301 cagagatagc cgaaggcttc gcggttggaa atcagcacac gcaggcgcgc aagcagtccg
     3361 taatggcggt ccagctgccg ctcgtcgcct tttcggataa agtggttgtc gcgttccaag
     3421 ctgttcttca ggcggaaata caggttttcg ctgatggcgg caaaacgtgg caaaagccat
     3481 aaaaacaacg caagtatgcc caccgccgac acgccgaccc aaaattccag caccagcagc
     3541 atgatgcacg cgccgaatat ggatacgacg gatgtcgcgg caatcggcag gtgttcttca
     3601 aaaaagctga caaactcacg cgacagggca acccgcgcag ttaccgccga atgcgggact
     3661 tgccgctgcc gctgttccaa cacaaccggc acggcgattt cggtataaat ccgcgtaaac
     3721 gtgcgcgtat cggcaatccg ccgcgccgca ccgaccagcc acatcaaaag cacaaccaaa
     3781 gcgtacagca acgcctgcca cgcctgcccc gcaatcacgg cattaatcgc ccagccgcca
     3841 aacaccggat acaccagcat caaaaggttt tccagtccga ccagaaaaaa tgtgccaatc
     3901 aatcgcttgc ggtggttttg gctatgtgtt tcaacatttt ccacatattt gcccccctat
     3961 tttttatttt ccgagattgt tttttccatc acttcagcca gtgcatccaa atcggcaaac
     4021 agccgggttg tgcgcttgtc gccgaatgtg gcaaatactt t
//
LOCUS       DAWEWP010000161         4022 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWEWP010000161 DAWEWP010000000
VERSION     DAWEWP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.161"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(214..285)
                     /locus_tag="WGN53_001789"
     tRNA            complement(214..285)
                     /locus_tag="WGN53_001789"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Arg,seq:cct)
     gene            complement(305..751)
                     /locus_tag="WGN53_001790"
     CDS             complement(305..751)
                     /locus_tag="WGN53_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ0140742.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(756..1730)
                     /gene="ispB"
                     /locus_tag="WGN53_001791"
     CDS             complement(756..1730)
                     /gene="ispB"
                     /locus_tag="WGN53_001791"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ0140743.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDGKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVDSDSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1746..1934)
                     /locus_tag="WGN53_001792"
     CDS             complement(1746..1934)
                     /locus_tag="WGN53_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140744.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLMEA"
     gene            1954..2262
                     /gene="rplU"
                     /locus_tag="WGN53_001793"
     CDS             1954..2262
                     /gene="rplU"
                     /locus_tag="WGN53_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ0140745.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2287..2559
                     /gene="rpmA"
                     /locus_tag="WGN53_001794"
     CDS             2287..2559
                     /gene="rpmA"
                     /locus_tag="WGN53_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ0140746.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIARQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            2794..3978
                     /gene="ubiM"
                     /locus_tag="WGN53_001795"
     CDS             2794..3978
                     /gene="ubiM"
                     /locus_tag="WGN53_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ0140747.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVS
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSNKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT         1151 a         1111 c          873 g          887 t
ORIGIN      
        1 aatttaatcc actatatcat aaaacaacag atttccaagc cggaacatct tttacgaagc
       61 ctgaaaatca tttcatgatt ctaccgtcct aaaggtcggt ttttcaagca ggaagaaaaa
      121 gttttcagat ggcaaaaaag ccctccaaca ctgaaaggcc ttatatcgga aacttcccgc
      181 aacacgggaa acagacaaat gcaaatcgtc aaacctcgcc aacaggaatc gaacctgtat
      241 tttacgctta ggaggcatac gttctatccg ttgaactatg gcgagccgaa atgaaaagga
      301 gattttaacc ctttccgacg acaaaagaca agatgccggc cgcaatcagc ggcccgacag
      361 ggataccgcc cataaatgcc accccgataa ccgtcccgat taacagccct gtaattaaaa
      421 caggctgctg tcccatcaaa ggcacgccgc gtcccgccag ccaagccacg aaaataccga
      481 taaaaacggc ggatatcatt ttaaaattca aaaattcggc aacgggagga acctgcgcct
      541 ttcctgaaac caacggactc aaaaccccta tggtcaaaag aatgataccg agattcaacc
      601 catgcttctc gaccaacggg acaaactgta ccaatgccgt ctgctgcatc agcagcaata
      661 tggttgccga gatggtaatc gaattgttgt tgctgaccac ccccaacaga atcagcgtaa
      721 ccagaaacag gggaacaaaa ctgaaattca tcgcctcaag acaccctgac caaagattcc
      781 ttcgccagct gaatcatggc atccttcact tcgctgtcgg gcagggcatc caaggcggca
      841 acggcacaat cgactgcttt gcgcgcctcg cctattgaat atgccaaagc atccgaacgg
      901 acgacataat cgtggatttt ctcaaaatag ctgcgatctg cattttccaa agcagtacgc
      961 acatcgttcg caacctgttc ggaaccctga cgcatcagat aaatcaaagg caaagtcggt
     1021 tttccttccg ccaaatcgtc gccgacgttt ttgccggttt cttcggtttc gccagaatag
     1081 tccagcacat cgtcaataat ttggaatgcc gtaccgacat acataccgta gtctttcaac
     1141 gcccgttcgt gttcggggga agccttgccc aaaattgcgc cgacttgagc ggcagcttca
     1201 aacaattttg ccgttttata ttggatgact tggatatatt gttcttcggt aatgtccgta
     1261 ttgccgatgt tcatcagctg catgacttcg ccctcggcaa taatgttggt tgcatccgcc
     1321 atcacttcca aaacgcgcat actgtccgag tcaaccatca gttgaaaggc gcgcgtgtat
     1381 aaaaagtcgc caaccaacac tgccgccgca ttgccgaaca gattgtttgc cgttgcccgt
     1441 ccacggcgca aatcgctttc atcgacgaca tcgtcgtgca gcagcgtcga agtgtggata
     1501 aattcgacca tcgccgccag cgaatacagt ttcccgtcat cataaccgac cgccttaccc
     1561 gccaaaatcg tcataatcgg acgcaggcgt ttgccgcccg cgctgatgat atatgtaccg
     1621 atttgcgaaa tcagtgcgac atcggattgc accgcacggt tgatgacttc attgactttg
     1681 gcaaggtctt caggcagatg tcgctggaaa tagggcagat tttcgagcat ggcagactct
     1741 caggtttacg cttccatcaa acggcagata ccgcttgaaa gctgattcaa atagcgggca
     1801 attatatcag caaagccatc agggtacatc ttttcagacg gcatatggtt atccgaaccg
     1861 cgcaaacttt gacaaaacag gcaaataaca atacaatgcc gagttccgca caatggtgtg
     1921 cggatattaa ccataaccct tatggagttg agtatgtacg cggtcgtaaa aaccggcggc
     1981 aaacagtata aagtttccgt cggcgaaaaa ttgaaagtag aacagatacc agccgaactc
     2041 gacagccaaa tcgaactgac cgaagttttg atgattgctg acggcgaatc tgtaaaagtt
     2101 ggcgcaccct ttatcgaagg tgcaaaagta acggctaaag tagtggcaca cggtcgtggc
     2161 gaaaaagtcc gcatcttcaa aatgcgccgc cgcaaacact accaaaaacg ccaaggccac
     2221 cgccaaaatt tcacccaaat cgaaatcgtg gcaatcgcct aaccttaaaa cttaggagca
     2281 ttacaaatgg caagtaaaaa agcaggcggc agcacccgca acggtcgcga ttcagaagcc
     2341 aaacgcttgg gcgttaaagc ctacggcaac gagctgattc ccgcaggttc catcatcgca
     2401 cgccaacgcg gtaccaaatt ccacgcaggc gacaacgtag gcatgggcaa agatcacact
     2461 ttgttcgcca aagttgacgg ttatgttgaa ttcaaaacca aaggtgcgct gaaccgtaaa
     2521 actgtcagca tccgtcctta caccggttct gaagaataat ccaaccgata cctgaagccg
     2581 catcttttca cgatgcggct ttttacatac cccacacccg atttgataca cattaagctg
     2641 aaagtaaaaa tccgcataca tcctcccctg catatttctt caacagcggg ttttgatata
     2701 atcgcctatc tgttacagat agttcaaacg gcattcaaac ccttacaaat gccgtctgaa
     2761 atcatcggca tccgtctatg caaaggatat tttatgagtt tacacagcga catcctcgtc
     2821 gtcggcgcag gcccggcggg tttaagtttt gcagccgaac ttgccggcag cggtttgaaa
     2881 gttaccctga tcgaacgcag cccactgact gttctgcaaa atcctccata tgacggacgc
     2941 gaaatcgccc tgacccattt ttcccgcgaa atcatgcagc gtttgggtat gtgggacaaa
     3001 attcccgaaa acgaaatcta ccctttgcgc gatgccaaag tgctgaacgg acgttccgac
     3061 taccagctcc actttcccca accgactgaa gcgcgcggcg aacctgccga ctgtttgggc
     3121 tatctgattt ccaaccacaa tatccgccgc gccgcctatg aagtcgtatc acaactcgac
     3181 aatgtcagca ttctgaccga tacggttgtc aaagaagtca aaacatctga caacgaagca
     3241 caagtcattt tggaaaacgg caaaatcctg actgcacgcc tgcttttggc ggcagacagc
     3301 cgcttctcgc aaacccgtcg acaactcggt atctcctccg atatgcacga ttacagccga
     3361 accatgttcg tctgccgtat gaaacatacc ctttccaacc aacataccgc ttacgaatgc
     3421 ttccattacg gacgcaccat cgcactgctg ccgctggaag agcacctgac caataccgtc
     3481 attaccgtcg ataccgataa aatcaacagc gttcaaaacc tttcgcccga agaattggcg
     3541 gcaagcgtaa aagaacaact caagggtagg cttggcgaca tggaactggt cagcagcatc
     3601 caccattacc cccttgtcag tatgattgcc aaacgtttct acggcaaacg cagcgcgctg
     3661 atcggcgatg cggcggtcgg tatgcaccct gtaacggcac acggcttcaa cttgggtctt
     3721 tccagcgcag atattctggc aaaactgata ctcgaagccg aacaacgcgg tcaggacatc
     3781 ggcgcatctt ccttgttgga aaaatacagc aacaaacata tgctgcacgc ccatccgctt
     3841 tatcacggaa ccaatatgat gctgaaactg tttaccaacg aaaccgcgcc ggcaaaactg
     3901 ctgcgcggtt tggtattgcg tgccggcaac aacttcccgc cgctgaaaaa gctgattacc
     3961 aaacaactga ccggttgaaa actgccgaaa tgccgtctga aacgtttcag acggcatttt
     4021 tg
//
LOCUS       DAWEWP010000162         3925 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWEWP010000162 DAWEWP010000000
VERSION     DAWEWP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.162"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..331)
                     /locus_tag="WGN53_001796"
     CDS             complement(<1..331)
                     /locus_tag="WGN53_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0140748.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVKEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQ"
     gene            complement(312..593)
                     /locus_tag="WGN53_001797"
     CDS             complement(312..593)
                     /locus_tag="WGN53_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ0140749.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(602..1396)
                     /locus_tag="WGN53_001798"
     CDS             complement(602..1396)
                     /locus_tag="WGN53_001798"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ0140750.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1780..3114
                     /gene="gdhA"
                     /locus_tag="WGN53_001799"
     CDS             1780..3114
                     /gene="gdhA"
                     /locus_tag="WGN53_001799"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ0140751.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3256..3630
                     /locus_tag="WGN53_001800"
     CDS             3256..3630
                     /locus_tag="WGN53_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140752.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3674..>3925
                     /locus_tag="WGN53_001801"
     CDS             3674..>3925
                     /locus_tag="WGN53_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ0140753.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQ"
BASE COUNT         1068 a         1014 c          924 g          919 t
ORIGIN      
        1 cttgcgcgat gccgttcaag gacaaaccca tacccttttc ggcaaaacgg atttcacggt
       61 cgtcaaacga gactttgccg aagccgaccc gtttcagggc ttccttcacg ctgttttgag
      121 gaggcggcgt ttccgcatcg ggacgggcgg caaaataatc ggcatacagt ttccacaacg
      181 cctgcactgt cggatcgaac gcgccgtccg tcagcgcgtg aatatcgcgg cacaggctca
      241 acagttccaa aaaatccgcc gacggcgaag tcagataacc gtccctgttc aggcggctga
      301 tcaggctgtc ttcacggtaa aggctgaaca attttttcca aacgcgccac ttccgccaaa
      361 accttgttga ccaaatccgc cgcctgtctg tcgtccacac cgaacaggcg cagctccgca
      421 ccggaaccca gcgcgacacc tttccagaag aacacatttt cattgcgttt ttcatcttta
      481 ttgcgttttt catcatcggc ggcaaaagga ttcggcagga aagaaaccgc cgcgcccgcc
      541 gccgcaacgg cggcaactgt caaaaaacgc ctgcgcccga aatgcctgcc cataccgcct
      601 cctaaaccga cactgccgcc ttgatgtgcg gatgggggtc gtaaccttcc aactcgaaat
      661 cttcaaactt gaaggaaaac aaatctttga cttcaggatt gattttcatc actggcaagg
      721 cgcgcggttc gcgttccaac tgcaatgcgg cctgctcgaa atggttgcgg tacaaatgcg
      781 cgtcgccaaa cgtatggaca aactcgcccg cctccaatcc gcacacttgc gccatcatca
      841 tggtcaacaa tgcgtagctg gcaatattaa acggcacacc aaggaaaata tctgcactac
      901 gctggtaaag ctggcaggac agtttgccgt cggcaacgta aaactgaaac agcgcgtggc
      961 agggcggcaa ggccatttcg tcgaccaaag ccggattcca cgccgataca atcaggcggc
     1021 gcgagtcggg gtttttctta atttgttcca ccacattggc gatttggtcg atatgcctgc
     1081 cgtcgggcgc gggccagtta cgccactggt agccgtaaac cgggcccaag tcgccgtttt
     1141 cgtccgccca ctcgtcccaa atggaaacat tgttgtcctt taggtatttg atattggtat
     1201 cgcctttgag aaaccaaagc agctcgtgga taatcgaacg cagatgcagc tttttggtcg
     1261 tcagcagcgg aaaacctttg cccaagtcaa aacgcatctg ataaccgaat acggagcgcg
     1321 tgcccgtacc ggtgcggtct gatttgtccg taccgttgtc gaggacgtgg cgcatcaagt
     1381 ccaaataggc tttcatagca gtctttcatc aaattaaacg gcgcatattg taacatttcc
     1441 ggataatgcc caaaacacgg atacagacag gcaggattgt tggcaatttc agtccttttc
     1501 cacagtaaaa cccggtggga aaacaaaatt accttgattg gaatcaaaaa atctagttta
     1561 attacttaga ataaaatttc aataatattt ttatttacga aattaattta tgattattga
     1621 ttaattatcg gcaacaaaca tcaaacatcg aaaacatgga aaaaataatg tcaacaattt
     1681 ttgccaaatc gggcttggca tcagaaaaaa ataggtttat attcccacct acaaatttgt
     1741 tttcccatta gtacactatc aaccaaaagg agtatccgaa tgactgacct gaacaccctg
     1801 tttgccaacc tcaaacaacg caatcccaat caggagccgt tccatcaggc ggttgaagaa
     1861 gtcttcatga gtctcgatcc gtttttggca aaaaatccga aatacaccca gcaaagcctg
     1921 ctggaacgca tcgtcgaacc cgaacgcgtc gtcatgttcc gcgtaacctg gcaggacgat
     1981 aaagggcaag tccaagtcaa ccggggctac cgcgtgcaaa tgagttccgc catcggtcct
     2041 tacaaaggcg gcctgcgctt ccatccgacc gtcgatttgg gcgtattgaa attcctcgct
     2101 tttgaacaag tgttcaaaaa cgccttgacc accctgccta tgggcggcgg caaaggcggt
     2161 tccgacttcg accccaaagg caaatccgat gccgaagtaa tgcgcttctg ccaagccttt
     2221 atgaccgaac tctaccgcca catcggcgcg gacaccgacg ttccggccgg cgacatcggc
     2281 gtaggcggac gcgaaatcgg ctatctgttc ggacaataca aaaaaatccg caacgagttt
     2341 tcttccgtcc tgaccggcaa aggtttggaa tggggcggca gcctcatccg tcccgaagcg
     2401 accggctacg gctgcgtcta tttcgcccaa gcgatgctgc aaacccgcaa cgatagtttt
     2461 gaaggcaaac gcgtcctgat ttccggttcc ggcaacgtgg cgcaatacgc cgccgaaaaa
     2521 gccatccaac tgggcgcgaa agtgctgacc gtttccgact ccaacggctt cgtcctcttc
     2581 cccgacagcg gtatgaccga agcgcaactc gccgccttga tcgaattgaa agaagtccgc
     2641 cgcgaacgcg ttgccaccta cgccaaagag caaggtctgc aatactttga aaaccaaaaa
     2701 ccgtggggcg tcgccgccga aatcgccctg ccctgcgcga cccagaacga attggacgaa
     2761 gaagccgcca aaaccctgtt ggcaaacggc tgctacgtcg ttgccgaagg tgcgaatatg
     2821 ccgtcgactt tgggcgcggt cgagcaattt atcaaagccg gcatcctcta cgccccggga
     2881 aaagcctcca atgccggcgg cgtagcaact tcaggtttgg aaatgagcca aaacgccatc
     2941 cgcctgtctt ggactcgtga agaagtcgac caacgcctgt tcggcatcat gcaaagcatc
     3001 cacgaatcct gtctgaaata cggcaaagtc ggcgacacag taaactacgt caatggtgcg
     3061 aacattgccg gtttcgtcaa agttgccgat gcgatgctgg cgcaaggctt ctaagcaaac
     3121 gccgccgctc cgcaaacaaa atgccgtccg aaccgcaaat gctgttcaga cggcatttca
     3181 ttatccgccc gttcgaatcg ggtgatacta tttgtatcca gaattgttgg atagtttggg
     3241 agtggagagg aagaaatgat tatggaccaa tactatatgg agttaaagaa taagttatct
     3301 aacagaccaa tcctactgga taatactaat gattttcttt ttgttttagt aaatacagtg
     3361 aaagctatga tagaaaatac tgataaaagt cagctttcag agttagataa aattttagac
     3421 ggtgtcacaa gtcaagaatt gaaacttgcc tatgattttt gtcaagggaa attcggtcaa
     3481 gcaggttttt cttatcgtag gcacccgaat tatttttatt tgtccagttt aattgcgact
     3541 tttccagaat ttgagttgtc taaagcagac cgagattacc tcaaaggaat cataaatttt
     3601 gataactacc ttttatatga gttagattag tctttaatca cctcaaaacc ctatttattc
     3661 aaaaggagaa aaaatgattg agctgaaatt agtagatgag agcagttttc aggcagtgct
     3721 ggatttgaaa atatctgaag ctgatgaacg agcacgtttt gtggctccaa atgtacgctc
     3781 tttagctgac gcatggctct accgggaaaa tgaagatgtg tttccgaggg caatctattg
     3841 ggataagcaa gtggttggct ttctcctgct ggaaatagac aaggatgaag cagaatactt
     3901 tatctggcgg ataatgattg gtcag
//
LOCUS       DAWEWP010000163         3921 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWEWP010000163 DAWEWP010000000
VERSION     DAWEWP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.163"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(499..1539)
                     /gene="queA"
                     /locus_tag="WGN53_001802"
     CDS             complement(499..1539)
                     /gene="queA"
                     /locus_tag="WGN53_001802"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ0140754.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVTVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGWNEGVVR"
     gene            1656..1913
                     /locus_tag="WGN53_001803"
     CDS             1656..1913
                     /locus_tag="WGN53_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ0140755.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPKHRQPLKEQKWICAN"
     gene            1895..2350
                     /locus_tag="WGN53_001804"
     CDS             1895..2350
                     /locus_tag="WGN53_001804"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ0140756.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKVGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2462..3823
                     /gene="accC"
                     /locus_tag="WGN53_001805"
     CDS             2462..3823
                     /gene="accC"
                     /locus_tag="WGN53_001805"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ0140757.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIEAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFNDPGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
BASE COUNT          966 a         1176 c          951 g          828 t
ORIGIN      
        1 acaaagccaa cgagtttttg ggtatgaccc gcctgccgac attcttatgt aacgatgtgg
       61 ttaaaaatcc gcaagtggaa aaatacttgg cagattatca ggcacacttg gaaaaagtgt
      121 tcggctaaaa atttatctta taaacaaaca aaggcagcct gaaagattga atggtctgca
      181 cccctaaggt tggactaacc aaccgactaa ggtgcagatt attttttgtt gctttttcag
      241 cttttcgttg ggttagatat tcttgcccac tgttttcagg cagccttgaa tacaaaaaat
      301 ggcgtatgta atatgtttat acgaccaaaa cggaatgaat tttaacgtat tgcgcgtcat
      361 caacaatgac tgagtttctc gcctctcgcg cctgaatcta tgtcatatac agttcctttc
      421 attcaaatca acaaaagaat gccgtccgaa cgtccgttca gacggcactt gtcttcccgc
      481 aatagacttg gggctgttct aacgtaccac cccttcgttc caccccaaaa ccatcgcatc
      541 gccgtagctg aagaaacggt attcgcgttc gaccgcataa cgatacgcgg cgcggatatg
      601 acccataccc gaaaacgcgc tgacaagcat cagcagcgtc gatttcggca aatgaaaatt
      661 ggtaaccagc ctgtcgacaa cattaaaacg gtagcccggc gtgatgaaaa tatcggtgtc
      721 gccctgcccc gctttcagac gacccgtcgc acgcgcggca gattcgaggg cgcgcatgga
      781 agtcgtgccg actgcccaga ctttgttccc ccgggctttt gccgcctcaa cggcggcggc
      841 ggtttcagac ggcacttcaa accattcgct gtgcatttta tgctcttcga ttttgtccac
      901 acgcacgggt tggaatgtcc ccgcaccgac gtgtagggtt acttctgcgg ttactgcgcc
      961 tttgtctttc aggcggcgca aaagttcttc cgtaaaatgc agacccgccg tcggcgcggc
     1021 gaccgcgccc tgatatttgg cataaacggt ttgataacgg ctgtcgtcat ccgcatcggc
     1081 ggcgcgttcg atataaggcg gcaggggcag gtgtccgttc tgttccaaaa gttcgtaaac
     1141 ggtttgaccg ccttcaaaac gcaggcagaa cagttcgccc tcacgcccga ccgtcacggc
     1201 gcggatgccg ccttcaaaca ccagccccat accgggcttg ggcgatttgg acgaacggat
     1261 gtgcgccagt gcggtatggt tgtccaacac gcgctcaatc agggcttcga tcctgccgcc
     1321 gctgtctttc tgcccaaaca gccgcgcctt catgactttg gtgttgttga acaccaaaac
     1381 gtcgcccgcc tcaacataat ccggcaaatc gccgaacacc cggtcttgca gcggcatatc
     1441 gggcaacgca accaaaaggc ggctgctgcc gcgcacttcg ggcggatgct gggcaatcag
     1501 cttttcgggc agggtaaaat caaaatctga aatatccatt tttacactct cgttcgggca
     1561 agccgccatt atacgcactt tagccctttt tcagacggca tctttgtccg aaaaaccaac
     1621 agattagaat aaacactctt aacccggggc atcttgtgcg caaaatcaaa cttcctgcac
     1681 atttccccca aaaaccgccg ttttttgata ttttactgga catttaccga caacttcggg
     1741 aaaataaaca cattctcacg gtcgttttcc accacaggaa aaccgtatcc gaacaccatt
     1801 ccgcccggtt tgcgccgttg ccgcaagccg gctgttttct gaaaaaccaa cgcaacaacc
     1861 cgccgaaaca ccggcagcct ttaaaggaac agaaatggat ttgcgcaaat taaaaaaact
     1921 gattgatttg gttgaagaat cgggtatcgc cgaaatcgaa gtaaccgaag gcgaggaaaa
     1981 agtccgcatc acccgaacca tcgccgccgc acccgtttac gccgcgcccg tacctgccgc
     2041 cgcgccggcc gtaacgcctg ccgccgcacc cgttgcggca tccgcgcctg ccgccgcacc
     2101 tgccgcccgc gatttgtccg acgcgcaaaa atcacctatg gtcggcacgt tctaccgcgc
     2161 acccggcccg aatgccgcgc cttttgtcga agtcggccaa caggttaaag tcggcgacac
     2221 gctgtgcatc atcgaagcga tgaagctgat gaacgaaatc gaagccgaaa aatccggcac
     2281 ggtcaaagaa attttggtcg aaaacggtac gcccgtcgaa ttcggcgaac cgctcttcat
     2341 catcggataa tcctgttttc agacggcata aacttccgat gccgtctgaa atgctttccc
     2401 ccttcagcgt tcccgcaccc ttttttacgg acgggttgcc ggaaccgcag gaaaggtcat
     2461 catgctgaaa aaagttttaa tcgccaaccg aggcgaaatc gcattacgcg tactccgtgc
     2521 ctgccgcgaa atgggcattg ccaccgtcgc cgtgcattcc gaggccgaca aagacagcct
     2581 gcacgtcaaa ctcgccgacg aatccgtgtg catcggccct gccgcttccg cgcaaagcta
     2641 cctcaacgtc cccgccatta tcgccgccgc cgaagtaagc tgcgcggacg ctgtccaccc
     2701 gggctacggt ttccttgccg aaaacgccga tttcgccgaa caggtcgagc agtccggctt
     2761 tacctttatc ggcccgaaac ccgacaccat ccgcctgatg ggcgacaaag tatccgccaa
     2821 acacgccatg atcgaagcgg gcgtgccttg tgttcccggc tctgacggcg cattgcccga
     2881 cgacggcgaa gaaatcctca aaatcgccga taaagtcggt tatcctgtca ttatcaaagc
     2941 ctctggcggc ggcggcggtc gcggtatgcg cgtggtcgag aaaaaagaag acctcctcca
     3001 atctgtcgaa atgaccaagg cagaagcagg cgcggcattc ggcaacccga tggtttacat
     3061 ggaacgctat ttgcaacgtc cgcgccacgt cgaaatccaa gtgattgccg acgaacacgg
     3121 caacgccgtc taccttgccg aacgcgactg ttcgctacaa cgccgccacc aaaaagtcat
     3181 cgaggaagca ccggctccgt tcatcactga aaaagaacgc gccaaaatcg gcaacgcctg
     3241 tgccgacgcc tgcaaacgca tcggctatcg tggcgcaggt acgtttgaat tcctgtacga
     3301 agacggcgaa tttttcttta tcgagatgaa tacgcgcgtt caggttgagc atccggttac
     3361 cgagctcatc accggcgtgg acattgtgca ggagcaactc cgcatcgcat ccggcctgcc
     3421 gctgcaatac aagcagaaag acatcaaaat cgaaggccac gcgtttgagt gccgtatcaa
     3481 cgccgaagac ccatacaact tcatccccag cccgggcccg attgaaagct gccacctgcc
     3541 cggcggcttc ggtatccgcg tcgacagcca catttaccaa ggctaccgca tcccgcctta
     3601 ctacgacagc ctgatcggca aaatctgcgt acacggcaaa acgcgtgaac aggcaatggc
     3661 gaaaatgcgc gtcgcactcg ccgagctggc ggtaaccggc atcaaaacca atacgccgct
     3721 tcaccgcgac ttgttcaacg acccaggttt ccaaaaaggc ggcgtcagca tccactattt
     3781 ggaacactgg ctggaagatc gcaaagccaa acaggacaaa taagccgccg ccgatatgcc
     3841 gtctgaaaca gtttgtccgt tttcagacgg catttccctt gccccgcgcc gtctgaaacc
     3901 gatttcgata tagtggatta a
//
LOCUS       DAWEWP010000164         3919 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWEWP010000164 DAWEWP010000000
VERSION     DAWEWP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.164"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            128..2713
                     /gene="acnB"
                     /locus_tag="WGN53_001806"
     CDS             128..2713
                     /gene="acnB"
                     /locus_tag="WGN53_001806"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ0140758.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHTL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFTSRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIADGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            2855..3850
                     /locus_tag="WGN53_001807"
     CDS             2855..3850
                     /locus_tag="WGN53_001807"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ0140759.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDG
                     IEYRGFAQETVEELTKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         1003 a         1140 c          991 g          785 t
ORIGIN      
        1 attacctgtc ataacagttc catttttgtt aaaactagtc tatgatagcg gtacaaatat
       61 tgtttacaat atttaacgca aatcatttgc aacccgacaa aagaaaaaca gaaaaaggaa
      121 caaagagatg ttagaagcct atcgtaaagc cgccgccgag cgcgccgccc tcggcattcc
      181 cgccctccct ttgaacgcgc agcaaaccgc cgatttggtt gagctgctga aaagcccgcc
      241 cgcaggcgaa ggcgagttct tggtcgaact gcttgcccac cgtgttccgc ccggtgtgga
      301 cgatgccgcc aaagtcaaag cctcattcct ggctgccgtt gccgaaggca gcgcgtccag
      361 cccgctgatc tcccccgaat atgcgaccga actcttaggt acgatgctcg gcggttacaa
      421 tatccacacc ttaatcgaac tcttggacga cgacaaactc gcgcccattg ccgccaaagg
      481 cttgaaacat acgcttctga tgttcgattc cttccacgac gttcaggaaa aagccgaaaa
      541 aggcaacaaa tacgcgcaag aagttttgca atcttgggca gatgccgaat ggtttacctc
      601 acgcgccaaa gttcccgaaa aaatcaccgt taccgttttc aaagttgacg gcgaaaccaa
      661 tacagacgac ctctcccccg cgcccgacgc gtggagtcgt cccgatattc cgctgcacgc
      721 gctggccatg ctgaaaaacc cgcgcgacgg catcacgccc gacaaaccgg gcgaagtcgg
      781 tccgattaaa ttgttggaag aactcaaagc caaaggccat ccggttgctt acgtcggcga
      841 cgtggtcggt actggttctt cacgcaaatc cgcgaccaac tccgtcattt ggcataccgg
      901 cgaagacatt ccgttcgtgc cgaacaaacg cttcggcggc gtgtgcttgg gcggcaaaat
      961 cgcgccgatt ttcttcaata cccaagaaga ctccggcgcg ctgccgattg aagtcgatgt
     1021 ttccgctcta aaaatgggcg atgtcgtcga tatcctgcct tatgaaggca aaatcgtgaa
     1081 aaacggcgag actgttgccg agtttgaatt gaaatcacaa gtattgctgg acgaagtgca
     1141 agccggcggc cgtatcaacc tgattatcgg ccgaggtctg accgccaaag cgcgcgaagc
     1201 cctgaaactg cctgcctcta ctgcattccg cctgccgcaa gcgcctgccg aaagcaaagc
     1261 cggtttcacc ttggcgcaaa aaatggtcgg ccgcgcctgc ggtcttcccg aaggacaagg
     1321 cgtgcgtccg ggtacttact gcgaaccgcg tatgacgacg gtcggctcgc aagacacgac
     1381 cggcccgatg acccgcgacg agttgaaaga cttggcttgt ttgggcttct ccgccgatat
     1441 ggtgatgcag tctttctgcc acaccgccgc ctatccgaaa cctgtcgatg taaaaaccca
     1501 taaagaactg cccgccttta tttccacccg tggcggcgtg tccctgcgtc cgggcgacgg
     1561 cgtcatccac tcgtggctca accgcctgct gctgcccgat accgtcggca ccggcggcga
     1621 cagccacacc cgtttcccca tcggtatttc cttccccgcc ggctctggct tggttgcctt
     1681 tgccgccgca acaggcgtaa tgccgctcga tatgcccgag tctgtattgg tacgcttcag
     1741 cggcaaactg caaccgggtg taaccctgcg cgatttggtg aacgccattc cgctgtacgc
     1801 aatcaaacaa ggtttgctga ccgttgccaa agccggtaag aaaaacatct tctccggccg
     1861 catcctcgaa atcgaaggcc tgcctgattt gaaagtggaa caagcctttg aattgaccga
     1921 cgcatccgcc gaacgctccg ccgccggctg taccgtgaag ctcaacaaag agccgattat
     1981 cgagtacatg aaatccaacg tcgtgttgat gaaaaacatg attgccgacg gctatcaaga
     2041 cccgcgtact ttggaacgcc gcatcaaagc catggaaaaa tggctggcaa atcccgagtt
     2101 gctcgaagcg gataaagatg ccgaatacgc cgccgtgatt gaaatcaaca tggacgacat
     2161 caaagagccg attatcgcct gcccgaacga cccggacgac gtgtgcttca tgtccgaacg
     2221 ctccggcacc aaaatcgacg aagtattcat cggttcgtgt atgaccaaca tcggccactt
     2281 ccgcgccgcc tccaaacttt tggaaggcaa ggcagacacc cccgtccgcc tgtggattgc
     2341 gccgccgacc aaaatggacg cgaaacaatt gtccgacgaa ggacactacg gcgtactcgg
     2401 acgtgccggc gcgcgtatgg aaatgccggg ttgctcctta tgtatgggta atcaggcgca
     2461 agtacgcgaa ggtgcgaccg ttatgtccac ctccacccgc aactttccga accgtttggg
     2521 taaaaacacc tttgtttacc tcggttcggc ggaattggca gcgatttgct ccaaactggg
     2581 taaaatcccg accgttgaag aatatcaagc caatatcggc atcatcaacg aacagggcga
     2641 taaaatctac cgctatatga acttcaacga aatcgacagc tacaacgaag tagccgagac
     2701 cgtgaacgtt taatccccgt catccgtatg aagtaaggga ttgaccgcaa tgccgtctga
     2761 acaaccttca gacggcattg caacattccg ctaacccttc tttccgcaaa cgctgcaaat
     2821 acggcgttca cgcccccaca taaaggaaac gacagtgaac ctgaaaaacc gccattttct
     2881 gaaactttta gacttcacgc cagaagaaat caccgcctac ctcgaccttg ccgccgaatt
     2941 gaaagccgcc aaaaaagcag ggcgcgagat tcagcggatg aaagggaaaa acatcgccct
     3001 gatttttgaa aaaacctcta ctcggacgcg ctgcgcgttt gaagtcgccg cgcgcgatca
     3061 aggcgcggga gtgacttatt tagagccgtc cgccagccaa atcgggcata aggaaagcat
     3121 caaagacacc gcccgcgtgt tgggcaggat gtacgacggc atcgaatatc gcggcttcgc
     3181 tcaggaaact gtcgaagaat tgacgaaata cgcgggcgta cccgtgttca acgggctgac
     3241 caacgagttc catcccacac aaatgcttgc cgacgcactg actatgcgcg aacacagcgg
     3301 caaacctttg aaccaaaccg cgtttgccta cgtcggcgac gcgcgttaca acatgggcaa
     3361 ttccctgctg attttagggg caaaattggg gatggacgtg cgtatcggcg caccgcaaag
     3421 cctgtggccg tctgaaggca ttattgccgc cgcacacgcc gccgccaaag aaaccggcgc
     3481 aaaaattacc ctgaccgaaa acgcgcatga agccgtgaag aatgttgatt ttattcatac
     3541 cgatgtgtgg gtcagcatgg gcgagccgaa agaagtctgg caggaacgca tcgatttgct
     3601 gaaagattac cgcgttacgc ccgaactgat ggcggcatcg ggcaatccgc aagtcaaatt
     3661 catgcactgc ctgcccgcct tccacaaccg cgaaaccaaa gtcggcgaat ggatttacga
     3721 aaccttcggg ctgaacggtg tggaagttac agaagaagta ttcgaaagcc ccgccagcat
     3781 cgtgttcgat caggcggaaa accgtatgca cacgattaaa gcggtaatgg tcgcggcttt
     3841 gggcgactga cagaactgtg cctgtttaaa ttcatccgca acacagatac cgtctgaaca
     3901 cgatgttcag acggtatcc
//
LOCUS       DAWEWP010000165         3898 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWEWP010000165 DAWEWP010000000
VERSION     DAWEWP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.165"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            362..2143
                     /locus_tag="WGN53_001808"
     CDS             362..2143
                     /locus_tag="WGN53_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ0140760.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDSALQKGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAENWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAQGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENGRCALLDRHLCRLKTSAQALNLPLPDGCENQIKQYIARLPDGAFRIKALLASDG
                     ISLSRAVLNRLTDKQRVIISPAVLPAQNYLRRFKTTCRALFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
     gene            complement(2202..3719)
                     /locus_tag="WGN53_001809"
     CDS             complement(2202..3719)
                     /locus_tag="WGN53_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0140761.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAEKLDLPAPVLENVGRFRKKAEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGS
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
BASE COUNT          932 a         1278 c          871 g          817 t
ORIGIN      
        1 caacgaagcc gaagacagta cagatagtac ggaaccgatt cacttggtgc ttcagcacct
       61 gagagaatcg ttctctttga gctaaagcga ggcaacgctg tactggtttt tgttaatcca
      121 ctatacttgg agctggtctt gcttttcgcc taattctacg ttttcaaacg gttgcagctg
      181 gtggtctgtc ataaaggtct ccttattgta tttcaggttg gaaatcggaa tttgttttca
      241 caattttaca ccttcgcccc ccactttctc tacataaaat tacattttgc cgatatttgc
      301 cgaatcgtct gaaaatatgt gtaaaaagag cgtataatca aaacatttgc cccgaattgc
      361 catgccttat ttcgccctgt ttgacgatgc cgtaagcggc cgcgcaaaac gctatcaaaa
      421 tcatgtggaa agccgttttt tccgtcccga agaactcgat gctttggaca gcgcgctgca
      481 aaagggctgg caaaaagggc tgcattcggt gttgtttgca gactacggat tcggtttgcc
      541 gctgacgggg gtcgagtccg aacgcggcgg caaccttgcc ctgcactggt ttgccgactg
      601 cgccgacacc gatgccgaaa actggcttgc ccgacattca gacggcctcc ccgccggcat
      661 ttccacgccg caatcctccg tatccgaagc cgattacctc gaccatatcc gccaaatcca
      721 cgaagccatc cggcgcggcg acacctatca aatcaactac accacccgcc tgcacctgca
      781 agcctacggc aatcccgtca gcctctaccg ccgcctgcgc cagcccgtcc cctatgccgt
      841 tttgtcccac ctgcccgatg cacaagggca atccgcgtgg acgctgtgtt tctcgcccga
      901 actcttcctc aaaatcggtt cggacggcac catcagcacc gaaccgatga aaggcaccgc
      961 gccgattttg ggcgacggac aagacgaacg ccgcgccgcc gagttgcaag cagacccgaa
     1021 aaaccgcgcc gaaaacgtga tgattgtcga tttgctgcgt aacgacctcg gcaaaatcgc
     1081 ccaaaccggc acagtatgcg tacccgaacc gtttaaagta tcgcgtttcg gcagcgtttg
     1141 gcagatgacc agcaccatcc aagcccaagc cttgccgcac acctcgttcg ccgacatcct
     1201 ccgcgccgcc ttcccctgcg gcagcatcac cggcgcgccc aaaaaaatga gtatgcagat
     1261 tatcgaatcg ctcgaagccg aagcgcgcgg actttatacg ggcagcatcg gctatttgaa
     1321 cccgtgttcc ggcggcttgg ggtttgaagg cacgttcaac gtcgttatcc gcaccttgtc
     1381 gctcacgccg ctttcagacg gcatttatca aggcgtgtac ggtgtcggtt ccggcatcgt
     1441 catcgacagc gaccctgccg ccgaatatcg ggaatgcggc tggaaagccc gtttcctcaa
     1501 cgaattgcgc cccgacttcg gcatttttga aaccctgcgc gtggaaaacg gacgctgcgc
     1561 cctgctcgac cgccacctat gccgtctgaa aacctccgcc caagccctca acctgcccct
     1621 gcccgacggt tgcgaaaatc aaatcaaaca atacatcgca cgcttgcccg acggcgcgtt
     1681 ccgcatcaaa gccctgctcg cttcagacgg catcagcctg tcccgcgccg ttttaaaccg
     1741 tctgaccgac aaacaacgcg tcatcatttc gcccgccgtc ctgcccgcac aaaactacct
     1801 gcgccgcttc aaaaccacct gccgcgccct cttcgaccaa gcgtggcaaa ccgccgaaac
     1861 acaaggcgcg ttcgacagcc tgtttttcaa ttcagacggc atcctgctcg aaggcggcag
     1921 aagcaacgtg ttcgtcaaac atcgcggaca atggctcacg ccctccttag atttagacat
     1981 tttaaacggc ataatgcgcc aagccgtatt ggacgaaccg caaaaatatt tgcaaacaaa
     2041 tcaagtaatc gaaacacaca tcacacaaaa aacactgcaa gaagccgaag aaatccgcct
     2101 ctccaacgcc ttgcgcggcg tatttgccgc cgcccttgcc tgaacgcgca aaaatgccgt
     2161 ccgaacctgt ttccaaagtt cggacggcat tatcccacca ttcaaaaccg ccaatccgcc
     2221 gacacaaaca cctcgctgtt gcggcgtttc gcatacggca cattactttc cgtcctgccg
     2281 aaacgataat tcaacgccgg cacgatacct ttgtacgaca acttgtcgtg gctcaaagcc
     2341 agcgagacat tccattcgcg gttgcgttgc gcctctgtcg agaaagccgc aatgccctta
     2401 tagttgcggc gggcataaga cgcagaaacc cgactgttca aaccgcccaa ctgccgccac
     2461 tcctgcgccc aaccggcata aacaccgttg cgccggtagg cggcattatt gaccgcgccg
     2521 cccaccgttt cgcgtttcgg cacaaaccgc acaaactgcc agccgccgaa cacagttgcc
     2581 gattcgccca aacgttttgc cgacgaaaca taaaacccgt cctgcctgcc gttattgtat
     2641 tccgccctat cctgttcgcg gtagcgttgg cggtaatgtt ccagcgcgac cgaaaattgc
     2701 catcccgggt ttgggcggta agtatgggac aactgcacgc cgactccgtg cgccagcata
     2761 tacggcggca ggcggcggtt gtttacccgt tttgttttcg catcaaagcc gtcgctgccc
     2821 gacaactgca cctgataaaa cggcaaaatc cccgccgtct gccgtgcatt tttatactgc
     2881 caacccaaat acgctctgcc gaacccgtcg tcataagctg attttttact gaaataatag
     2941 ctcgtgccgc cgatattgga acggaacaac aaataatgat tatctgccaa cgccgtcagt
     3001 ttttccgcct cgatttcata attcaagcct gccgcccgct ccgcccggct gacactgcat
     3061 atctgactgc ctccgttttg ccggcaatac tgcggcgcgg cattattggc atttctattg
     3121 accgccgggc tgataccgcc cgaaaaacgc cagcccgtca gcccctccgc ttttttccga
     3181 aaacgcccca cattttccaa aaccggcgcc ggcaaatcca atttttccgc ctcggcaaaa
     3241 tgcctttctg ccgacttcag ccggaaatcg tcaaactccg ccgccgccaa atccagcaaa
     3301 atccgctcgt ctgccgcatt ttccccgtgc agttcccgat accgcgccac cgcctccgcc
     3361 ggccttcccg ccaatttcgc cagcaaagcc cgcgccctgc cgtacaaaac cgcgtcataa
     3421 tccggcagct tggcatacaa atccgccaac gaagcgatta aatccgcctg attgccgttg
     3481 agcgcgtcgc gcaaactatg ttccaacatt ttcgggtgcg ccaacaaaaa atccccgtca
     3541 accacgcgcg gggcatcatt ttcaactttc caatccgatt ccgcccactt atccgacacc
     3601 gaccgctgca cctgcaacaa tgccttgtca tccaaaatcg cgggcgcatc cgccccatag
     3661 gcggcagaaa cacctgccgc acaccaaaca accaaaaaac cgtatctgaa atacaacata
     3721 ccctgtcatt tacctttctg gcaaacacgc cgccgaagca cgtcaaacca tccgaaaaac
     3781 aggcagaaac ccgtgaaaac cggctttgcc gtctgaaagc gggcaggcaa aaaaccgccg
     3841 cccgattttc aaagggcgga tttcacattt atagtggatt aacaaaaatc aggacaag
//
LOCUS       DAWEWP010000166         3888 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWEWP010000166 DAWEWP010000000
VERSION     DAWEWP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.166"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..2121
                     /locus_tag="WGN53_001810"
     CDS             <1..2121
                     /locus_tag="WGN53_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0140762.1"
                     /translation="GKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFGG
                     ESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTASVQVGSAQLIKGASTAVNGMNPE
                     GAVSGSVNIETKKAADEGNRKIGLGRFSNNRAQGTFDLGQRFGENKAFGVRANGKLRH
                     GDTPRHGYSEDNKEFAVNADYRGETLRVAFDSIYAKRKTNGGRARMQDIQNAGGRLFG
                     APDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVCL
                     NATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLSARGEFETGPVTHN
                     WSTAFDRVIRQRKTINGSGNGNSKIEVKANENIANQLASFTADYPNSWENSANLDANI
                     KVNSLALSDTLGFVDNKYRLTLGGRFQAVEYTNKKKSQSGDAKRFSPMLMAAWVPQPD
                     LVVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKRP
                     GYWRGHTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANLL
                     NNTLRPTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLNG
                     NVSYFGKSYQDTQKQYAFPSYTLVDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQR
                     GQYDRSFAVVGMPRTYWLKAELDF"
     gene            2245..3795
                     /locus_tag="WGN53_001811"
     CDS             2245..3795
                     /locus_tag="WGN53_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ0140763.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEA
                     GMVSEPQQ"
BASE COUNT         1100 a          955 c          980 g          853 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcgccgatt accgtcgtca actacgacga acaagccctc
       61 aacaacaccg aagcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gagaaagcaa cacgctgact ggactgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggtttggca ggtatgtacg gcacgcaagg cacggccagc
      241 gtgcaagtcg gctccgcaca actgattaaa ggcgcgtcca ccgccgtaaa cggcatgaac
      301 cctgaaggtg cggtatccgg ttccgtcaat atcgaaacca aaaaagccgc cgatgaaggc
      361 aaccgcaaaa tcggtttggg ccggtttagc aacaaccgcg cccaaggtac gttcgatttg
      421 ggtcaacgct tcggcgaaaa caaagcattc ggcgtacgcg ccaacggcaa actgcgccac
      481 ggcgacactc cgcgccacgg ttacagcgaa gacaacaaag aatttgcggt aaatgccgac
      541 tatcgcggcg aaacactgcg cgtagcgttc gattccatct acgcaaaacg caaaaccaac
      601 ggcggtcgcg cgcgtatgca ggacattcaa aacgccggcg gacgcttgtt tggcgcgccc
      661 gatggtaaaa ccaacctgct gccttcttgg aactggcaaa ataccgccgg ccaaaccaat
      721 atgcttacgt ttgaatggga tgcgtttgat aatgcacaga ttacaggcgg tatcggctac
      781 aacaaggcac gctattacgg cacgctgatt tccccgaccg tatgtttgaa tgctaccagc
      841 acttgtaccg atatgcaaaa cacaaacgcc agcggcaaag taacggcacg caactatgac
      901 tacaataccg gtactgcgcg cctgaccgac caatacttcc gcactttaag catgaacctg
      961 tctgcgcgag gcgaatttga aaccggcccg gtaacacata actggagtac ggctttcgac
     1021 cgcgttatcc gccagcggaa aaccataaac ggcagcggga acggcaacag caagatagaa
     1081 gtgaaggcaa acgaaaatat tgcgaatcaa ttggcatcgt ttacagccga ctatcccaat
     1141 tcttgggaga actctgccaa cttggatgcc aatatcaaag tcaacagcct ggctttgtcc
     1201 gacaccttgg gcttcgtgga taacaaatac cgcctgactt tgggcggacg tttccaagcg
     1261 gttgaataca ccaataagaa aaaatcacag agcggcgatg ccaaacgctt cagcccgatg
     1321 ctgatggcgg cgtgggtgcc gcaacctgat ttggttgtgt acggcaacta tatggaagac
     1381 ttggagcctg ccgatatcaa aaccgatgat tccggcgaaa cgacaatggc gaaaccgcgt
     1441 gtcagccgtc agtttgaagt gggcgtgcgt aaaaactggg gcaatttcgt aaccacgtta
     1501 aacgcgttcc aaatcaaacg cccgggctac tggcgcggtc atacaactac caagacaaca
     1561 aaaggggtaa caacaacgct gacctatggc aacaattccg atttcgcccg ctataaggcg
     1621 caaggcggcg cggccggcga cgagcaaggt atggaacgca atcgcggtat tgagttcaac
     1681 acctacgcga acttgctcaa caatactttg cgtccgactt tgggtctgat gtatctgcaa
     1741 tcgaccgtga aagagtatcc aaattcacgc gatatgctgg ttaacggcgt acaagtcgcc
     1801 aatccgcgcg taattgccaa agcaggtttg gaatgggata caccgtttgc caaaggattg
     1861 actttaaacg gtaatgtttc gtatttcggc aagtcttacc aagacacgca aaaacaatac
     1921 gctttcccgt cttacacttt ggttgacgta ggcgcgcgct acaaaaccaa gctcggcaaa
     1981 aatacgttga ccgtcagcag ctcggtggaa aacctgttca acaaaaacta ctggcaggta
     2041 cagcgcggcc aatacgaccg cagcttcgcc gtcgtcggta tgccgcgtac ttactggttg
     2101 aaagcggaat tggatttctg atttctccgc atccggaaaa tgccgtccga acaggtttca
     2161 gacggcattt cttttaaaga aatatggcgg gatatgctta aataatgcta ttctagcgtc
     2221 tttgaaacct gcttgtttga agtgatgaaa aaacctttga tttcggttgc ggcagcattg
     2281 ctcggcgttg ctttgggtac accttattat ttgggtgtca aagccgaaga aagcctgacg
     2341 cagcagcaaa aaatattgca ggaaacgggc ttcttgaccg tcgaatcgca ccaatatgag
     2401 cgcggctggt ttacctctat ggaaacgacg gtcatccgtc tgaaacccga gttgctgaat
     2461 aatgcccgaa aatacctgcc ggataacctg aaaacagtgt tggaacagcc ggttacgctg
     2521 gttaaccata tcacgcacgg cccttttgcc ggcggatttg gcacgcaggc gcacattgaa
     2581 accgagttca aatacgcgcc tgaaacggaa aaagttttgg aacgcttttt tgggaaacaa
     2641 gctccggttt cccttgccaa taccgtttat tttaacggca gcggtaaaat ggaagtcagt
     2701 gttcccgcct tcgattatga agagctgtcg ggcatcaggc tgcattggga aggattgacg
     2761 ggaaaaacgg tttatcaaaa aggtttcaaa agctaccgca acagctatga tgcgcccttg
     2821 ttcaaaatca agctggcaga caaaggcgat gccgcgtttg aaaaagcgca tttcgattcg
     2881 gaaacttcag acggtatcaa tccgcttgct ttgggcagca gcaatctgac cttggaaaaa
     2941 ttctccctag aatggaaaga gggtgtcgat tacaacgtca agttaaacga actggtcaat
     3001 cttgttaccg atttgcagat tggcgcgttt atcaatccca acggcagcat cgcaccttcc
     3061 aaaatcgaag tcggcaaact ggctttttca accaagaccg gggaatcagg ctcatttatc
     3121 aacagtgaag ggcagttccg ttttgacacg ctggtgtacg gcaatgaaaa atacggcccg
     3181 ctggacatcc atatcgccgc cgaacatctc gatgcttctg ccttaaccgt attgaaacgt
     3241 aagtttgcac aaatttccgc caaaaaaatg accgaggaac aaatccgcaa tgatttgatt
     3301 gccgccgtca aaggcgaggc ttccggactg ttcaccaaca atcccgtatt ggacattaaa
     3361 acttttcgtt tcacacagcc gtcgggaaaa atcgatgtgg gtggaaaaat catgtttaaa
     3421 gacatgaaga aggaagattt gaaccaattg ggtttgatgc tgaagaaaac cgaggcagac
     3481 atcagaatga gtattcctca aaaaatgttg gaagacttgg cggtcagtca ggcaggcaat
     3541 attttcagcg tcaatgccga agatgaggcg gaaggcaggg caagtcttga cgacatcaac
     3601 gagaccttgc gcctgatggt ggacagtacg gttcagagta tggcaaggga aaaatatctg
     3661 actttgaacg gcgaccagat tgatactgcc atttctctga aaaacaatca gttgaaattg
     3721 aacggtaaaa cgttgcaaaa cgaaccggag ccggattttg atgaagccgg tatggtttca
     3781 gagccgcagc agtaatgggt caaatgccgt ctgaaatgtt caggcggcat tttgttgtat
     3841 tggaaactgc cgttttcaac cattccttta gataagccgt gtcggatt
//
LOCUS       DAWEWP010000167         3863 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWEWP010000167 DAWEWP010000000
VERSION     DAWEWP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.167"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WGN53_001812"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WGN53_001812"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ0140764.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WGN53_001813"
     CDS             1439..1924
                     /locus_tag="WGN53_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ0140765.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGN53_001814"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGN53_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ0140766.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          788 a          944 c         1076 g         1055 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ccgtcagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgccga tgtcaaacct
      661 gaggacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggcgcg
      721 ataccgcgtt gggtgttgtt aagtgcggct ttgcccgaat tggatgaggt atggctggaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcattgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgt gttaccggtt tatatttaat ccgtttcggt ttcgcgcctt cttcgccgag
     2041 tcggcgtttc ttatcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgcttacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgccta
     3781 tccctttgaa attatacaga caaaaaaaat aataatgata ggagatcgcc gcaccggcaa
     3841 ccatttcgga ttttccaaag caa
//
LOCUS       DAWEWP010000168         3854 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWEWP010000168 DAWEWP010000000
VERSION     DAWEWP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.168"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(83..277)
                     /locus_tag="WGN53_001815"
     CDS             complement(83..277)
                     /locus_tag="WGN53_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ0140767.1"
                     /translation="MKHKLSSWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(267..2348)
                     /locus_tag="WGN53_001816"
     CDS             complement(267..2348)
                     /locus_tag="WGN53_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ0140768.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DSKQPEAQSEA"
     gene            2824..3204
                     /locus_tag="WGN53_001817"
     CDS             2824..3204
                     /locus_tag="WGN53_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ0140769.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGRRGYMPPVQNQAGPNDF
                     RAFSCENGLSVRVRRLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3337..3830)
                     /locus_tag="WGN53_001818"
                     /pseudo
     CDS             complement(3337..3830)
                     /locus_tag="WGN53_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
BASE COUNT          905 a          958 c         1091 g          900 t
ORIGIN      
        1 cttgtcctga tttttgttaa tccactatat tataaaaatt ccgtctgaag cggcgttcag
       61 acggaatttg tttcaagcag gcttaacagc agcgtccgcc gtttgcgccg cagcggcgtt
      121 tgcggcaata gtcttgaaac tgcagcttgg tcatcacagg ggcgttggga ttatgtttgc
      181 gctgctgtgc aacgtagttt tcataatcgg gcacgcctgc catcaagttt gctgtcagct
      241 tgatggtttt ccaccaagac gagagcttat gcttcacttt gtgcctccgg ctgtttgctg
      301 tcgcggtaca ccgccgggat ttctttggcg gtcggccagc cgactttgcg tgctttgagg
      361 gcggtacgca aaccgtacgc ggcgacaatc acgacaaccg acaagaagag gatggtcaga
      421 ccggcattaa tcttgtcgtt gaagatgatt tgcgccattt cgccgatgtc tttggcaggc
      481 gcaaggactt cgtttttagc caatgcgtcg ctgtatttgc cggcgtgggc aaggaagctg
      541 atgcgcgggt cgctgtggaa cagtttttgc aggccggcgt agcaggttac gaacagtacg
      601 ccgacggcgg gaacgagtac cacccaaaca taacggtcgc gtttcatctt aatcagcacc
      661 acggcgcaca taatcaaggc cacgcctgcc agcatttggt tggcgatgcc gaacaaaggc
      721 cagagcgagt tgatgccgcc cagcgggtcg gttacgccgg tgtagaggaa gtagccccac
      781 aatgccacgg cgaagaaggt cgcaatcagg ttggcgggga tggagtcggt gttgccgaaa
      841 ggtttgtaga agatgctgcc caagtcttga atcataaaac gtgcgacgcg cgtaccggca
      901 tcgacggcgg tcaggatgaa caaggcttca aacaataggg cgaagtgata ccagaacgcc
      961 atcatcgcct cgcccggaat caggcggctc ataatgtgcg ccataccgac tgcgagggtt
     1021 ggcgcaccgc ctgcacggga aaggatggtg ttttcgccca cttccttagc ggtatgcaac
     1081 agggttgccg catcgacagg gaattgcagc ttggtggtaa tcacttcggc ggcggtgttg
     1141 gcatccgtac cgatcagggc ggcagggctg ttcatggcga agtacacgcc gggatcaagc
     1201 gatgcggcgg cggcaagcgc cataatggct acgaaacttt ccatcaacat accgccgtaa
     1261 ccaatcatac ggacgtgggt ttcgttttcc agcattttcg gcgtggtgcc ggaggaaatc
     1321 agcgcgtgga agcccgaaac cgcaccgcag gcgatggtga tgaagaggaa cgggaacaat
     1381 gcgcctgaga ataccggacc cgaaccgtcg ataaagtggg ttacggcagg catttgcaaa
     1441 gtgggattga cgatgacgat gcccaaagcc aaggccgcaa tcgtaccgat tttcaggaag
     1501 gtggagagat agtcgcgcgg agtgagcagc aaccatacgg gcaatacgga ggcgacaaag
     1561 ccgtaaatca taatcgccca agtgagctgg atgccgtcaa ggtcgaacca atgtccgatg
     1621 gaacttttcg ccacatcttc gccgtaaatt accgccagca tcagcaaaat aaagccgacg
     1681 atggaaatct cgccgatttt gcccggacgg atatagcgcg tgtaaatacc cataaacagg
     1741 gcaatcggca tagtggcggc gatggtaaac gtaccccaag ggctgtgaac aagtgctttt
     1801 acgacaatca acgccaacac cgccataatg atgaccataa tcatcaaaat accgatggag
     1861 gcaatcacgc cggggacagt gccgagttcc tgtttcacaa tatcgcccaa agacttaccg
     1921 tcacggcgca tagaaacaaa cagtaccatc atatcctgta ccgcgccggc aaaaaccacg
     1981 ccgaagataa tccacaaagt accgggcagg taacccattt gcgcagccag tacagggccg
     2041 accaaagggc ccgcgccggc aatcgcggca aagtggtgtc cgaacaatac gcccttgtgc
     2101 gtcggaacgt agtccaagcc gtcgttgtgg cgttctgccg gagtcaggcg gttaggatcg
     2161 agccgcatta cgcggttggc gatgtagagg ctgtaaaaac ggtaggcgat gcagtaaacg
     2221 gatatggcgg cggtaaccat ccataccgcg ctgacctgtt cgcctcggct gagagccaga
     2281 gtggtaaagg atgctaagcc gaccagtacc actatgcccc aaatgaggaa ggttttgagt
     2341 gatttcatcg aataaatcct tatctcacac tgtcggaata tgcctgaacg gcgggaaacg
     2401 gatgaccgcc tgccggcacg ggcacaaacc ggagtctgaa tgattgacga agtatgaaac
     2461 agtgcgcttg tcgggatttg tgccttatgc ccgcatcaaa cagtgcaggg atgcgggcgc
     2521 acaaaacgtt aaacgccgaa taggatttta acgcaaatta gcacactgat agcggttttt
     2581 acttggaatt tggaaaaaat ttacattcct tcgggcggac aggcgggttt ggatggcatc
     2641 gtcaggcaaa aggcggcgcc ggaagacggg taaagaaggg gcgcgcaatc cggattattg
     2701 attcatcgcg ggtaaattcc ggttatcggg cttgtgtgtt tgcgcgtccg tttatagtat
     2761 ggcgttgccg cagcttggaa tcagggcggt tgtttcatat cttattttta ttggggagct
     2821 tttatgaata tcaggttttt cgcgctgacc gtaccggttt tgtctttggc ggcctgtgcc
     2881 gtgccggagg cgtatgatga cggcagacgc gggtatatgc cgcctgttca aaaccaagcc
     2941 ggcccgaacg attttcgggc gttttcatgc gagaacggtt tgtctgtgcg cgtccgccgt
     3001 ttggacggtg gcaaaatcgc gttgcggctg gacggcaggc gtgccgtcct ctcttccgac
     3061 gttgccgcat ccggcgaacg ctataccgcc gaacacggtt tgtttggaaa cggaaccgag
     3121 tggcaccaga aaggcggcga agcctttttc ggctttaccg atgcctacgg caattcggtc
     3181 gaaacttcct gccgcgcccg ttaaacgggg cagcctgttt gaaccgccgt ccggattttt
     3241 cgggcggttt tttgtgtcgg atttgttttg gctgtcggct ggtttcggcg gttttcgggc
     3301 gtggcggatt atttttcgtt tcgttccggg tttatcctat cggcagcggc tcaatgccaa
     3361 ctttaaacct gctccgattt cttcagggct gttatccaat gataaaatga catcgtctgc
     3421 atcaatggca tcccacgctt ccagcttgac atggcggctc gggctgattt tcaggcagcc
     3481 gttgtgcagc caaatatccc cgctcatcat gtttttaaat agggcgcgtc tggttttata
     3541 gcccaagttc ccgcatagtt tggcaaccca atcctcatag cgttgccgaa atttttcggt
     3601 atcaaaaaaa tcttggtctt ccgaactgtc ataaacgaaa gtcctgctgt ttgccaacgc
     3661 ttgcaagacc gtcgtgccta aagtttcatt gtcggtatcc aatggcagga tatgggggga
     3721 tataggtggt ctattgccgt taaccccaaa cctgaacatg tttgaacaat cagagcttct
     3781 tcattagcat aaaaaactgc ccaataattt tgatcctgtt taaaaattat tatttgatct
     3841 ccgtaatttt gatt
//
LOCUS       DAWEWP010000169         3852 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWEWP010000169 DAWEWP010000000
VERSION     DAWEWP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.169"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(267..2699)
                     /gene="hupB"
                     /locus_tag="WGN53_001819"
     CDS             complement(267..2699)
                     /gene="hupB"
                     /locus_tag="WGN53_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ0140770.1"
                     /translation="MHIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTDTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYAPKILASNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPELKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDNPNKPTYAPLSDGTALVSSP
                     VWQNQNRTAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(2730..3757)
                     /locus_tag="WGN53_001820"
                     /pseudo
     CDS             complement(2730..3757)
                     /locus_tag="WGN53_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein"
BASE COUNT          705 a          893 c         1206 g         1048 t
ORIGIN      
        1 ggtgcaggtt tctgtgcgga tagcttcgtc attcccgcgc aggcgggaat ccaggttcgt
       61 tgagtttcag tcatttccga taaattcttg cagctttgcg tttctagatt cccacttccg
      121 tgggaatgac ggagagtcgc gggaatgacg gagagttgcg ggattgggct tggtacaagc
      181 ctgaaagggg gcggcggtgc gaccgtaaaa aatgccgtct gaaacccaaa tcatcgggtt
      241 tcagacggca ttggcacatc aggagcttag aacttcgctt cgatggtgaa attgtagctc
      301 ctgcccggcg aggtaaagcg ttggatgccg gcgtgggttt tgttgtcaac gcggttgacc
      361 gtgccgaact cgcggatgct gcgtaaagat tcccaagtgt agtactgctt gttggtaatg
      421 ttgtacgcgg cggcgcggag cgtaacctgt ttgccgatgt tgaggtaggc ggaaaggtcg
      481 aacagcgtat aggccttgct gtgtttggca taaggccacg ggttgttcaa gtcgtcgttg
      541 ctgtggacgg tgtcggacgg ctttttggcg gcggtgcgcg tggcgtaggc gttgatgccc
      601 caacgtttgg aaggagcgtc gtagcccagg ctgtaaaccg ccgtccacgg cgaaagcgcg
      661 ttgatgggcg tttctttgcc gttgttttgc gttgccttgc ccttgatgta gctgacgttg
      721 aggccggtgt gcagcccttt gggcagcccg atgctgtcga ggttccaagt gccgttaaac
      781 tctatgcctt ttacccaggc ggcggtgcgg ttttggtttt gccaaacggg cgagctgacc
      841 aatgccgtac cgtctgaaag cggggcgtag gtgggcttgt tagggttgtc tgacgaaacg
      901 cccatgtagg tcagttcgat aaagtcgcgg tatttggttt tgaagcccga gagcttgaag
      961 ttgcccgctt tgccgctgcc cgccaggccc aattcccagt ttttggcttt ttcggctttc
     1021 agttcggggt tggctttcag gtagaaatcg gggtgtggga acagcagcca ggtttcgtcc
     1081 gaagtcggcg cgcggaagcc ggtgctgtat tttgccaaca agtgcagatg cttggtaaac
     1141 cgccagtcga accccgtgcc gtagctgaag cccgcgtgcg cgcgttccga acccaaatgg
     1201 ggaatctgcc cccggatggc ggtggtgtat ttcggatcgt ctttcgcgct gctgctgttt
     1261 ttgtcgtagc gtatgcccgc attcaggcgg aagcggtcgt tgccgcccaa gtgaaacgca
     1321 ttgttccaat aggcaaattt atatttcgac cggttttcga tcagcattgt gattttggct
     1381 tggttggaag cgaggatttt gggggcgtac agttttgcga aatagctgta atccgagttg
     1441 gcattgtccc ctctgccgcc gcccaagccg tattgcgccg cccaaacggc tttggaaaag
     1501 tcgagttgtt tttcaaaatc ggcagtccat tgcgcggtgt tttggcggat atgccggaac
     1561 gaatggtaaa cctcgccgtt gatgccgcgc aaatcgtaat ttttcgggat gtcccaagtc
     1621 caagtgttca tatcgatgcg ctgcttgtcg tagcgcagct tgaggctgtc ccacgggccg
     1681 tgttccaatt cgtttttgta ttcgacaccg gagcggcggc ggtagctcac gtcttggcgg
     1741 tggcggtagt cgcccgttgc ggcagacgtg gtcgtatccg tccacaaatt agacagctcg
     1801 ttggtaaaac ggtcggtgcg cgagtcttca aaaatccagc cgatacggtt ttgatcgttg
     1861 aagttgtagc ccagcttgaa cagggtgctt ttgtttaccc attcctgtgg gtcgggttgg
     1921 gagcgcgcaa cccctgtggc tacataggtc aggtacttgc tgggtccgcc tgtatcggtc
     1981 gggttataga catatccgtc gtttttaatt tcgacatcgc cctcagtcga gcggtttttg
     2041 gtttctttgc cgaagcggcg ggtataaacc aataaggcat ccaaaccaaa gagcctgccg
     2101 gcggcggtga tgctgctgaa tttttggctg tttttgccga cgctgccgcc ctttatcccc
     2161 aaatggtagg gcttgtcttc ggaaacataa tcgcttgcgg atttggtttg gtaattgact
     2221 gcgccgccca atgcgccgct gccggatttg agcgagtccg cgcctttggt gatggttact
     2281 tcggaaaagt tttccggctc ggaagtgttg cggttggcgt tgaagttgcc gtacgcgccg
     2341 aacaattctt ggaaagcttc ggaagagcgg ctttccgcct gcgccagccc gtcaacgttg
     2401 atggcgacgc ggtctttgtc cacgccgcgt atggtaaagc cgttagaacc cgccctgccg
     2461 ccttcgacga cggaaatgcc ggggtcgtag cgcaccaggt cgtgttcgtc gttgaccaag
     2521 agcttgtcta aggttttgcg gcggattttt tcttcgccga gtttttgggt tttgtgcgtg
     2581 ccggtaacgg tgatttcgtt cagcgtttgg gcggactgcg gcgcggggtc tgccgcaaag
     2641 gcgggaaacg cttgggcgat ggcggcggca gccaatacgg gtttgaaggg gatgtgcata
     2701 agaaaacctt tttaaaagcg gattaattat tattttgtga gatgggtggt ctctcgatcg
     2761 gtggtgcttt caagttcttt tttataactc acgccgccga atacggtatc gagggagcgg
     2821 tcgccgtcaa aggtgatttt gccgcctatg ctggtgtcgc tgtcatagct gctgttgaat
     2881 ttgccaaaaa acttgccttc caatttgccg gttttgccgc cggaggtggc atcgccggaa
     2941 aaggataatc cgtcaatctg tgcttcttta atttccacat ccggcccgta atcgctgttg
     3001 ccgatgattt tgcctgccag tttgttactg ttgaaattgg cggtgataaa ggaaaggacg
     3061 ggggtgttgg taggattgaa cgtataacca ctgtttttcg gcggcgtata agaggaagta
     3121 acaaattgcc cgtctttgac gcggatgccc caaacctgat aagtgatttt gcccttgccg
     3181 gcggtttcgg gcgtgtcgtc gctccaggag tatgccggtt tcggcgtttt gccgacgggg
     3241 aagccgccgg cgaagaggtc gatttcgccg ttgggaccta tccacgcccc gaatttcata
     3301 taatcttgtt tggtgatttc cgcatacgaa gtagccccgg attcgctgca acagataaag
     3361 atgcggctgc cgtcggcaag ccgctgccct gcgtggcggt cgggaatttt gaaatcgggg
     3421 gaggagtagc tgacggttat ggggtcggcg taggatgaaa gggcgtattg cgcgccgccg
     3481 ggtgtttgga agatgaatga tgtttctttg gttttgtagc cggggaaagg ttctttttct
     3541 acttcccgct gctgaatgga aataccggat gtgcttcctg tgccggcggt gttgatgttt
     3601 tcgattttgc tattgtcgtc tgataacttt acctcgccgg tgggcagcgg ggtggcggtg
     3661 gggatggaca cggggatgtg cggttcggct accccccccc tgcacaggcg gcaagggcgg
     3721 cggcaagcgg cagtaaggac agggctttgt atttcatcgc atactcaatc ttgatgatgt
     3781 gttaataata gttttgatta tcagtttaaa tgttatatga tgtcaactgg ttttgtgggt
     3841 cggtgcaaaa at
//
LOCUS       DAWEWP010000170         3851 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWEWP010000170 DAWEWP010000000
VERSION     DAWEWP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.170"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(88..906)
                     /locus_tag="WGN53_001821"
     CDS             complement(88..906)
                     /locus_tag="WGN53_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0140771.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTGLSDRLPLSVLTGSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(973..2223)
                     /locus_tag="WGN53_001822"
     CDS             complement(973..2223)
                     /locus_tag="WGN53_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0140772.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTTALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRNIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="WGN53_001823"
     CDS             complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="WGN53_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ0140773.1"
                     /translation="MSDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            2871..3683
                     /locus_tag="WGN53_001824"
     CDS             2871..3683
                     /locus_tag="WGN53_001824"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ0140774.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          847 a          966 c         1136 g          902 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcgggaaa tatccatatc
      121 aggctgggga ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggag aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatgcagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaaag gattttctat tttgggagct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttagataa aggggtgtag tctgtcccgc ttatggtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttcgg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggctcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactat atacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gcccgttaaa acagataaag gaagccgatc ggacaatccg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacta
      961 ccgccttttc aatcaacccc gcttcaatac ggatgtattg atgtagcgtt ggacacccga
     1021 ggcaatggat tgggcgcact gccggcggaa ggattcgctg cccagcaact tctcttcagc
     1081 agggttggac aggaaggcgg tttcgaccag gatagacggc atatcgggtg cgcgcaaaac
     1141 ggcgaaattg gcttcgtcca ccctgccttt gtgcaagtgg ttgagcctgc ccaattcttc
     1201 aagcaccagt ttgccgagtt tgcggctgtc gcgcagcgtg gcggtttggg tcatatcgag
     1261 cagggcggta tcgacgttgc ggttgccgct ggtcggtacg ccgccgaccg cgtcggcatt
     1321 gttttgcgtc tgttccaaga atttggcggc ggagctggtc gcgcctttgg tgttcagcat
     1381 atagaccccc gttccgcgcg cggaggggct ggtgaaagcg tcggcgtgga tggagacgaa
     1441 tacgtccgcc cgccgtgctc gccctttggc gacacgcacg cccaatggga tgaacacgtc
     1501 ttcgttgcgc gtcataaaga cgttgtaacc caaggcttcc aattgttttt tggtttcgcg
     1561 ggcgatggag aggacgacgt gtttttcctg caaaccgccc gggctgacgg cgccggggtc
     1621 ttcgccgccg tgtccggggt cgatcataat gacgggtctg cgcccgtttt tgccgcgttc
     1681 ggtttgtgcg ggagtgtttt gagcgaggtc ggcttcaggg gagccgcgac gggttttgtt
     1741 caggctgccg ttaagcagcg ccatcatcgg atcgtcggga tccatccctt gcggatagag
     1801 atcgacgacg aggcggtttt taaagccgcc gacggggggg agggaaaaaa cttgcgcgta
     1861 ggaaggctgt ttcaaatcga tgacgaggcg gacggtgtcg ggcgtgttct gaccggcgcg
     1921 tatattgcgg ataaaggggt cgtccgccat aaccttctga gacagtccgt gcaatacggt
     1981 attgatgttc gcgttttgta tgtcgacgac caacctgccc gggttgtcga gcgtgaagtg
     2041 ttggtatttg agcgcggttg tgctttccag cgtcaggcgg gtgtaggtgt gcgacggcca
     2101 tatccgtacg gcggtaaact gcggggcgcg taccgttttg gcaacggcgg atgcgatggg
     2161 gcttagggcg aacagtgtgc cggcggtgcg gcggatgatt tgtcttcgtg tcagtttggt
     2221 catagcggca ggctttcgcg tcctcgttcg gtatgggcgg tcagcaggca ttttctgccg
     2281 ccgccgtcgt gtgtcaatgt tgcggtgatg tcggcgggcg gggtaaattc gcctccctgt
     2341 tgcggccatt cgatcaggca gacgctgttt gcggcaaaca gttcgtcaag ccccgcgtct
     2401 tcccattctt cggggaacga gaagcggtag aggtcgaaat ggtgcagggt gaagcgttcc
     2461 agcggataag attcgacgat ggcgtaggtc gggcttttga ctgcgccctg atgacccaat
     2521 ccgcgcagga tgccgcgtgt cagcgtggtt ttgcccgcac ccaaatcccc ttcgagataa
     2581 atgaccagcg gtgcgtttaa acgggaagac cacgccgcac ccaaatcgag tgtggcggct
     2641 tcgtcggcaa ggaatcggga aacgggagga aaatcagaca taggggcttt acaactttta
     2701 tgtggcggat cggaggggca ggcggtcggc aaaccgtgcg cccggcggca gcattgccgt
     2761 acattataac agccaaatcc gcatcctgct ttcagacggc aacggctgtc aagaaaaagc
     2821 ggcgcgtgta caatacgcgg attgtatgtt taggacggat tggaaaaaga atggaaaata
     2881 tcggcaggca gcgacccatc ggcgtttttg actcgggaat cggcggtttg accaatgtgc
     2941 gagcgctgat ggaacggctg ccgatggaga acatcattta tttcggcgac acggcgcgcg
     3001 tgccttacgg gacgaaatct aaggcgacca tcgaaaattt ctcgatgcag attgtcgatt
     3061 ttttattgga acacgatgtc aaggcgatgg ttatcgcgtg caatacgatt gcggcggtgg
     3121 cggggcagaa aatccgtcaa aaaaccggca atatgcccgt tttggacgtg atttccgccg
     3181 gcgcgaaagc cgcgctggca acgacgcgca acaataaaat cggcattatc gccaccaata
     3241 cgacagtcaa cagcaacgcc tatgcgcgcg ccatccacag gaacaacccc gatacgctcg
     3301 tccgcacgca ggccgcgccg ctgctcgtcc ctttggtgga ggaaggttgg ttggaacatg
     3361 aagttacccg cctgaccgta tgcgaatacc tcaaaccatt gcttgcggac ggcatcgata
     3421 cgctggtgtt gggctgcacg cactttccct tgctcaaacc cttaatcggc agggaggcgc
     3481 acaatgtcgc actggtcgat tccgcgatta cgactgccga agaaaccgca cgcgtccttg
     3541 cgcaggaggg attactcaat accgacaaca acaatcccga ctaccgtttt tatgtcagcg
     3601 atattccttt gaaattcaga accatcggcg agcgttttct gggcaggacg atggagcaga
     3661 ttgaaatggt gtctttgggt tagcggcagg tacggacgga aaccgtcccc gtccgcgcca
     3721 tccgcatacg cacccgcaac gccctgccgt attaaacgcc ccgtcgccgc aaatgccgtc
     3781 tgaaaggctt cagacggcat tttttacaca attcccaccg tttcccatcc ttctcgatac
     3841 actgtaatct c
//
LOCUS       DAWEWP010000171         3802 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWEWP010000171 DAWEWP010000000
VERSION     DAWEWP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.171"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            354..683
                     /locus_tag="WGN53_001825"
     CDS             354..683
                     /locus_tag="WGN53_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0140775.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGTSAPDFNAGG
                     TGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPESAY
                     RRLYKPK"
     gene            697..3558
                     /locus_tag="WGN53_001826"
     CDS             697..3558
                     /locus_tag="WGN53_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEZ0140776.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGGGKDIKASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLS
                     APYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIG
                     AVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKG
                     GSQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIV
                     YLADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAG
                     YLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRG
                     NAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMP
                     GIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLD
                     HNGTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGRSTW
                     SENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGT
                     LMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGL
                     AGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVA
                     GLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1029 a         1031 c         1009 g          733 t
ORIGIN      
        1 atagtacgga accgattcac ttgttaaaga atcgttctct ttgagctaag gcgaggcaac
       61 gccgtactgg tttttgttca tccactataa ctagggaaat tcaaattaag ttagaattat
      121 ccctatgaga aaaagccgtc taagccggta taaacaaaat aaactcattg agctatttgt
      181 cgaaagttca aatttccatt ttaaaacaat tagtaaaatc gagtttatcc tagttgtcca
      241 agacaacccc tataataata taattcaaaa tataaaaatg ggttacatct aaacattacg
      301 gaatttttat tccctcgcct gaattctatt gtcagattca aggagacctc atcatgcgaa
      361 cgaccccaac cttccctaca aaaactttca aaccggctgc catggcgtta gctgttgcaa
      421 caacactttc tgcctgctta ggcggcggcg gcggcacttc tgcgcccgac ttcaatgcag
      481 gcggcaccgg tatcggcagc aacagcagag caacaacagc gaaatcagca gcagtatctt
      541 acgccggtat caagaacgaa atgtgcaaag acagaagcat gctctgtgcc ggtcgggatg
      601 acgttgcggt tacagacagg gatgccaaaa tcaatgcccc cccccccgaa tctgcatacc
      661 ggagacttta caaacccaaa tgacgcatac aagaatttga tcaacctcaa acctgcaatt
      721 gaagcaggct atacaggacg cggggtagag gtaggtatcg tcgatacagg cgaatccgtc
      781 ggcagcatat cctttcccga actgtatggc agaaaagaac acggctataa cgaaaattac
      841 aaaaactata cggcgtatat gcggaaggaa gcgcctgaag acggaggcgg taaagacatt
      901 aaagcttctt tcgacgatga ggccgttata gagactgaag caaagccgac ggatatccgc
      961 cacgtaaaag aaatcggaca catcgatgtg gtctcccata ttattggcgg gcgttccgtg
     1021 gacggcagac ctgcaggcgg tattgcgccc gatgcgacgc tacacataat gaatacgcat
     1081 gatggaacca agaacgaaat aatgtctgca gccatccgca atgcatgggt caagctgggc
     1141 gaacgtggcg tgcgcatcgt caataacagt tttggaacaa catcgagggc aggcactgcc
     1201 gaccatttcc aaatagccaa ttcggaggag cagtaccgcc aagcgttgct cgcctattcc
     1261 ggcggtgata aaacagacga gggtatccgc ctgatgcaac agagcgatta cggcaacttg
     1321 tcctaccaca tccgtaataa aaacatgctt ttcatttttt cggcaagcaa tgacgcacaa
     1381 gctcagccca acacactgac cctattgcca ttttatgaaa aagatgctca aaaaggcatt
     1441 atcacagtcg caggcgtaga ccgcagtgga gaaaagttca atggctccaa ccattgcgga
     1501 attactgcca tgtggtgcct atcggcaccc tatgaagcaa gcgtccgttt cacccgtaca
     1561 aacccgattc aaattgccgg aacatccttt tccgcaccca tcgtaaccgg cacggcggct
     1621 ctgctgctgc agaaataccc gtggatgagc aacgacaacc tgcgtaccac gctgctgaca
     1681 acggctcagg acatcggtgc agtcggcgtg gacagcaagt tcggctgggg actgctggat
     1741 gcgggtaagg ccatgaacgg acccgcgtcc tttccgttcg gcgactttac cgccgatacg
     1801 aaaggtacat ccgatattgc ctactccttc cgtaacgaca tttcaggcac gggcggcctg
     1861 atcaaaaaag gcggcagcca actgcaactg cacggcaaca acacctatac gggcaaaacc
     1921 attatcgaag gcggttcgct ggtgttgtac ggcaacaaca aatcggatat gcgcgtcgaa
     1981 accaaaggtg cgctgattta taacggggcg gcatccggcg gtagcctgaa cagcgacggc
     2041 attgtctatc tggcagatac cgaccgatcc ggcgcaaacg aaaccgtgca catcaaaggc
     2101 gatctgcagc tgggcggcga aggtacgctg tacacacgtt tgggcaaact gctgaaagtg
     2161 gacggtacgg cgatgaccgg cggcaagctg tacatgtcgg cacgcggcaa aggggcaggc
     2221 tatctcaacc gtaccggaca acgtgttccc ttcctgagtg ccgccaaaat cgggcgggat
     2281 tattctttct tcacaaacat cgaaaccgac ggtggtctgc tggcttccct cgacagcgtc
     2341 gaaaaaacag cgggcagtga aggcgacacg ctgtcctatt atgtccgtcg cggcaatgcg
     2401 gcacggactg cttcggcagc ggcacattcc gcgcccgccg gtctgaaaca cgccgtagaa
     2461 cagggcggca gcaatctgga aaacctgatg gtcgaactgg atgcctccga atcatccgca
     2521 acacccgaga cggttgaaac tgcggccgcc gaccgcacag atatgccggg catccgcccc
     2581 tacggcgcaa ctttccgcgc agcggcagcc gtacagcatg cgaatgccgc cgacggtgta
     2641 cgcatcttca acagtctcgc cgctaccgtc tatgccgaca gtaccgccgc ccatgccgat
     2701 atgcagggac gccgcctgaa agccgtatcg gacgggttgg accacaacgg cacgggtctg
     2761 cgcgtcatcg cgcaaaccca acaggacggt ggaacgtggg aacagggcgg tgttgaaggc
     2821 aaaatgcgcg gcagtaccca aaccgtcggc attgccgcga aaaccggcga aaatacgaca
     2881 gcagccgcca cactgggcat gggacgcagc acatggagcg aaaacagtgc aaatgcaaaa
     2941 accgacagca ttagtctgtt tgcaggcata cggcacgatg cgggcgatat cggctatctc
     3001 aaaggcctgt tctcctacgg acgctacaaa aacagcatca gccgcagcac cggtgcggac
     3061 gaacatgcgg aaggcagcgt caacggcacg ctgatgcagc tgggcgcact gggcggtgtc
     3121 aacgttccgt ttgccgcaac gggagatttg acggtcgaag gcggtctgcg ctacgacctg
     3181 ctcaaacagg atgcattcgc cgaaaaaggc agtgctttgg gctggagcgg caacagcctc
     3241 actgaaggca cactggtcgg actcgcgggt ctgaagctgt cgcaaccctt gagcgataaa
     3301 gccgtcctgt ttgcaacggc gggcgtggaa cgcgacctga acggacgcga ctacacggta
     3361 acgggcggct ttaccggcgc gactgcagca accggcaaga cgggggcacg caatatgccg
     3421 cacacccgcc tggttgccgg tctgggcgcg gatgtcgaat tcggcaacgg ctggaacggc
     3481 ttggcacgtt acagctacgc cggttccaaa cagtacggca accacagcgg acgagtcggc
     3541 gtaggctacc ggttctgacg gacaggaagc agacagccgc aaagatcacg gtctttgcgg
     3601 ctgtttctta tgaaaagaaa accctattcc aattgcctgc ttctattgtt tcaagacttc
     3661 ttccaaagat tcggcattaa tcagatgtat agcggattaa caaaaatcag gacaaggcgg
     3721 cgaagccgca gacagtacaa atagtacgga accgattcac tcggtgcttg agcaccttag
     3781 agaatcgttt ctttgagcta ag
//
LOCUS       DAWEWP010000172         3781 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWEWP010000172 DAWEWP010000000
VERSION     DAWEWP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.172"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..187)
                     /locus_tag="WGN53_001827"
     CDS             complement(<1..187)
                     /locus_tag="WGN53_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0140777.1"
                     /translation="MKPDTLQSYGSTTRFLHWTIAACYLFMFATVIAWNIDENLKFLI
                     NSHKAVGILLLILSVWRV"
     gene            457..867
                     /locus_tag="WGN53_001828"
     CDS             457..867
                     /locus_tag="WGN53_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0140778.1"
                     /translation="MNHIARQFAILLGEGLKPLGIAPAQFPILLELWQKDGLSQQELV
                     ERADLKQATIANTLARMERDGLITREPNPDDARSRLIMLTEQARALQQQSTEIAQAIN
                     QTALSDLSANEQKLFLEMAAKIVARQQEMITGEK"
     gene            complement(982..1533)
                     /locus_tag="WGN53_001829"
                     /pseudo
     CDS             complement(982..1533)
                     /locus_tag="WGN53_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520560.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(1574..2233)
                     /gene="ung"
                     /locus_tag="WGN53_001830"
     CDS             complement(1574..2233)
                     /gene="ung"
                     /locus_tag="WGN53_001830"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ0140779.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            2314..2721
                     /locus_tag="WGN53_001831"
     CDS             2314..2721
                     /locus_tag="WGN53_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ0140780.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIANP"
     gene            2736..3683
                     /locus_tag="WGN53_001832"
     CDS             2736..3683
                     /locus_tag="WGN53_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ0140781.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
BASE COUNT          929 a          937 c          971 g          944 t
ORIGIN      
        1 ctacgcgcca aacagacaat atcagcagca ggattccgac tgctttgtgc gaattgatta
       61 gaaatttcag gttttcgtcg atattccacg caatgacggt agcgaacata aacaggtagc
      121 aagctgctat tgtccaatgc aggaagcggg ttgttgagcc gtagctttgc aaagtgtcgg
      181 gtttcatgat gctttctctt gataaagggt tagtcggatt tcggtatggc ttacagatag
      241 gaaaaccttc tttaaagtat tgaagttcat aagtgttctt gaacatttaa gataggtcgt
      301 ctgaaagtgt ttagatagca agctattaag ctgcaataag tatagcgtgc tatgtattaa
      361 gatagcaagc tatatttgtg atataatccg caacaatttg atttgaaaga aaacaaagtg
      421 gacgaagttt tcaaaactga atcggcaggc tatttgatga accacattgc ccgccaattt
      481 gccattttat tgggcgaagg tttaaagcct ttgggcatcg cccccgcgca atttccgata
      541 ttgctggaac tgtggcaaaa agacggtttg agccagcagg aattggtgga acgcgccgat
      601 ttgaagcagg caacgattgc caacactttg gcgcggatgg agcgcgacgg actgattacg
      661 cgcgaaccga atcccgacga tgcgcgcagc cgcctgatta tgctgacgga gcaagctcgc
      721 gccttgcagc agcagtccac ggaaattgcc caagccatca accaaacggc tttgtcggat
      781 ttgtcggcaa acgaacaaaa gttgtttttg gaaatggcgg caaaaattgt ggcgcgacag
      841 caagagatga ttacgggcga gaagtgaggg ggccgatggc gcaacttgga attttaaagt
      901 gcaggctgct tttgaggtcg tctgaaagca gcctgcgctt tgaaaacaag gtgcaggctg
      961 cttagggtaa tgtgtcgttt atcaatccag tttcaggatt tctccgtctt ccggaacgtt
     1021 cacacggctt tcaatgcctt gaccgcgtat aaatttacgc atatcggtgc ggctgacggc
     1081 ggtatgattc acggtatcca tgtgtacggt aatgcctgaa atcagcgcgt agcccgtgtt
     1141 cataatcaga taatcgggtt tgtagcggtt caatgctttg tttacatctg ccgtccacac
     1201 agtatcaccc ataatataag cggttttgtg tccgctgctt tggaatacta cacccattgc
     1261 atcacctaag atttcggcta gctgcgggtt ggcatacatg gcttctgtac catgtacgcc
     1321 gccggttttg ctgatggtta cgccgttgaa gacagtgctg ccgttcaaca cgcgtacatc
     1381 agtaaatcct tggctgcgga tttttgccgc gtcggcttgg tgctgcacat atacgggcag
     1441 gtttttcgga atggaacgtg cggcggtttc gccccaatgg tcttcgtggg tatgggtaac
     1501 gataactgca tccacaccgt agattgactg gttccataag tgcagtttaa gcaacttggc
     1561 attcagacgg cattcacagc ttccagttta tcggatcgat accgtgccgg ctcaaatagc
     1621 tgtttgcctg tgaaaaatgg cggcagccga aaaaaccgcg atatgccgac agaggagacg
     1681 gatgcggtgc ggtcaatatc aaatgatttt gactgtctat cagcctccct ttttgttgtg
     1741 catacccacc ccacaacatg aagacaaggt gcttgcggtg tgtcgcaagc tgcctgatga
     1801 cggtatcggt aaagcgttcc cagcctaaaa gggcgtgcga atgcgcctgt cctgcacgca
     1861 ccgtcaaaac cgtgttcaga agcaatacgc cctgctccgc ccacgctgtc agacagccgt
     1921 gcgcgggaat ggaaaagcct tcgatgtcgg tttccaactc cttgtagata ttgagtaaag
     1981 acggcggtat gcggataccc tgccggacgg aaaatgccaa accgtgcgcc tgccctgccc
     2041 cgtgatacgg atcttgtccg agaatgacgg ctttgacccg gtcgaacgct gtcaggcgga
     2101 atgcgttgaa cacatccgcc gccggcggat agatgatttg tcccgacaaa cgttcctgcc
     2161 tgactgcatt taaaatttcc tgaaaatacg gctgctgttt ttcgccgccg agtgcatcgt
     2221 gccaagtgtc catatctgcc ctatcctttc aaattcggct atactgctga tattgcgtta
     2281 ttttgtcaaa cgacgcgctt tgtggggaaa tgtatgactg tatggtttgt tgccgctgtt
     2341 gccgtcttaa tcatcgaatt attgacggga acggtttatc ttttggttgt cagcgcggct
     2401 ttggcgggtt cgggcattgc ttacgggctg accggcagta cgcctgccgc cgtcttgacc
     2461 gccgctctgc tttccgcgct gggtatttgg ttcgtacacg ccaaaaccgc cgtgggaaaa
     2521 gttgaaacgg attcatatca ggatttggat gccggacaat atgtcgaaat cctccgacac
     2581 acaggcggca accgttacga agttttttat cgcggtacgc actggcaggc tcaaaatacg
     2641 gggcaagaag agcttgaacc aggaactcgc gccctaatcg tccgcaagga aggcaacctt
     2701 cttatcatcg caaaccctta acactcggag gaattatgga atttttcatt atcttgttgg
     2761 tagccgttgc cgttttcggc ttcaaatcct ttgtcgtcat cccacagcag gaagtccacg
     2821 ttgtcgaaag gcttggacgt ttccatcgcg ccttgacggc cggtttgaat attttgattc
     2881 cctttatcga ccgcgtcgcc taccgtcatt cgctgaaaga aatcccttta gacgtaccca
     2941 gccaggtctg catcacgcgc gacaacaccc agctgacggt tgacggcatc atctatttcc
     3001 aagtaaccga tcccaaactc gcctcatacg gttcgagcaa ctacattatg gcgattaccc
     3061 agcttgccca aacgacgctg cgttccgtta tcgggcgtat ggagttggac aaaacgtttg
     3121 aagaacgcga cgaaatcaac agcaccgtcg tctccgccct cgatgaagcc gccggagctt
     3181 ggggcgtgaa ggttttgcgt tatgagatta aagacttggt tccgccgcaa gaaatccttc
     3241 gctcaatgca ggcgcaaatt actgccgaac gcgaaaaacg cgcccgtatc gccgaatccg
     3301 aaggtcgtaa aatcgaacaa atcaaccttg ccagtggtca gcgcgaagcc gaaatccaac
     3361 aatccgaagg cgaggctcag gctgcggtca atgcgtcaaa tgccgaaaaa atcgcccgca
     3421 tcaaccgcgc caaaggtgaa gcggaatcct tgcgccttgt tgccgaagcc aatgccgaag
     3481 ccatccgtca aattgccgcc gcccttcaaa cccaaggcgg cgcggatgcg gtcaatctga
     3541 agattgcgga acaatacgtc gctgcgttca acaatcttgc caaagaaagc aatacgctga
     3601 ttatgcccgc caatgttgcc gacatcggca gcctgatttc tgccggtatg aaaattatcg
     3661 acagcagcaa aaccgccaaa taagccgtga tgaaaatgcc gtctgaagct ctgttttcgg
     3721 ggttcagacg gcatttttat ttaaacccgg gcaaggttgt attccgcttc aaattatagt
     3781 g
//
LOCUS       DAWEWP010000173         3719 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWEWP010000173 DAWEWP010000000
VERSION     DAWEWP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.173"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..179)
                     /locus_tag="WGN53_001833"
     CDS             complement(<1..179)
                     /locus_tag="WGN53_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0140782.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGINKNTAAYYFHR
                     LRLLIYQNSPHLEMF"
     gene            440..1912
                     /gene="pyk"
                     /locus_tag="WGN53_001834"
     CDS             440..1912
                     /gene="pyk"
                     /locus_tag="WGN53_001834"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0140783.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     LLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            2220..3620
                     /locus_tag="WGN53_001835"
     CDS             2220..3620
                     /locus_tag="WGN53_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0140784.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          973 a         1096 c          924 g          726 t
ORIGIN      
        1 tcaaacattt ccaaatgcgg actgttttga taaataagta atcgtaaacg atgaaaataa
       61 taggctgcgg tatttttatt aatgcctact aactctgctg ccgttcttgc agttacacct
      121 gcgacaaaca gttcaatgag tttattttgt ttataccggc ttagacgact ttttctcata
      181 gggataattc taacttaatt tgaatttccc tagttatcta ggacagtccc aaaaataaat
      241 ttagcctaag tcataaaacc gtgtaatttt gcaccaaaaa aggcggtttg caggtaaaat
      301 atcaaactat taatttaaat catattttta cagaatattc cgccgcgttc atcaaatgga
      361 catcaaaccg gttccaaatt tctgtaattt tgtaacaaaa taccgcaaaa cacccgattg
      421 agaccaaaag gactttcata tgaaccagac aagccgcgat ctgacccgca tcagccacaa
      481 caccaaaatc gtcgccaccc ttgggccggg cagcaacaac gtcgaactgt tggaagatat
      541 gatccgcgtc ggcggcctga acgtcgtccg cttcaacttc agccacggca cgcccgaatt
      601 ccatcaggaa aacgcccgca tcgtgcgcga agcggcaaaa cgcgccggac aggaaatcgc
      661 catcattgcc gacctgcaag ggccgaaaat ccgcgtgggc aaaatcgccg gcggcggcat
      721 cgaattgaac aaaggcgaaa cgctgctact ggatgccgcg cttgaaggcg aaggcacgcg
      781 cgaggcggtc ggtttggact accgcgacct gcccgatgac gttgccgcag gcgatgtctt
      841 gtggctggac gacggcctgc tgaccctgac cgtggaatcc gtcgaaggca gcaggattat
      901 cacaagggtg gaaaacagcc acgtcctgaa aagcaacaag ggcatcaaca aacgcggcgg
      961 cggtctgtcc gcaggcgcgt tgaccgaaaa agacttccgc gacctgaaaa ccgcgattgc
     1021 catcggttgc gactacctcg ccatcagctt tgtgaaatcc gccgaagatt tgcatatcgc
     1081 gcgcgccaaa gtcgaagaag aaatgaaagg cagcacggcc gtgcgccccg gtttggtttc
     1141 caaaatcgaa cgcgtagaag cgattgaaaa cttggacgaa atcatcctcg ccggcgacgg
     1201 cattatggtt gcgcgcggcg acttggcggt cgaagtcgga cacgccgccg tccccgccct
     1261 gcaaaaacgg atgatccgcc gcgcccgcga gttgcgccgc ttcagcatta cggcgacgca
     1321 aatgatggaa tcgatgatta ccaaccccgt accgacccgc gcggaagtca gcgatgtggc
     1381 aaacgcggta ttggacggta ccgatgcggt gatgtgttcc gccgaaaccg ccgtcggcgc
     1441 gtatccgttt gaaaccgtca gccaaatggc aattatctgc gcggctgcgg aaaaagagca
     1501 ggattcgctc aacggcgttg ccgaacaggc ggagtatccc gaagcggtca gcaccaacct
     1561 ggcggttgcc ggcggcgcgg tcagcgtggc gcgcgcggtt cacgccaaag cgattgtcgc
     1621 cctgaccgaa agcggttcga ccgcctttga aatcagccgc cacaacatca ccctgccgat
     1681 tttcgcgctg accccgagcg tttccgccca acgccgtatg gcgatgtacc gcggcgtgcg
     1741 cccgctgatt ttggcaacca gtaccgacca cgacacggcg ttgaacgaag tcgaaaccat
     1801 gctggtggaa cacaacatcc tgcattccgg cgaccagtac atcatcacca gcggttcgca
     1861 aatgcgcgaa tccggttcga ccaacacact ggaagtgctg cgcgtcaaat aatccgccct
     1921 gagtggaaaa tgccgtctga agccgatgcc cgaggcttca gacggcattt tttgcggcgg
     1981 cgcggcggtt cgggcaaact gaaaaaattg tgcaaatccg gcaacatcgg ataaaattga
     2041 gtacctatac taaagcgaaa caaggcattt ccgactgcct tttttatagt ggattaaatt
     2101 taaaccagta cggcgttgcc ttgccgtact atctgtactg tctgcggctt cgtcgccttg
     2161 tcctgatttt tgttaatcca ctatatttat ccaccgtccg cctttttacg gaaaccgaaa
     2221 tgaccccttc cgcactgaaa aaaaccgtcc tactgctcgg cactgccttt gccgccgcat
     2281 ccgcacaagc ctccggctac cacttcggca cacagtcggt caacgcgcaa agcacggcaa
     2341 atgccgccgc cgcagaagcc gccgacgcat cgaccatctt ctacaaccct gccggcctga
     2401 ccaaactcga cagcagccag atttccgtca acgccaacat cgtgctgccc agcattcatt
     2461 atgaggcgga ttccgccacc gactttaccg ggcttcccgt ccaaggttcg aaaagcggca
     2521 aaatcaccaa aaccacggtc gcgccccaca tctacggcgc atacaaagtc aacgacaatc
     2581 tgaccgtggg cttgggcgtg tacgtcccct tcggctctgc caccgaatac gaaaaagatt
     2641 ccgtgttgcg ccacaacatc aacaaactcg gtctgaccag catcgccgtc gaacctgtcg
     2701 ccgcatggaa actcaacgac cgccattcct tcggcgcagg catcatcgcc caacatactt
     2761 ccgccgaact gcgcaaatat gccgactggg ggattaagag taaagcagag atattgacgg
     2821 caaaaccgcc caaacctaac ggtgtagccg aagctgcaaa aattcaggcc gacggacacg
     2881 ccgatgtcaa aggcagcgat tggggcttcg gctaccaact ggcgtggatg tgggacatca
     2941 acgaccgcgc gcgcgtgggc gtgaactacc gttccaaagt ctcgcacacg ctcaaaggcg
     3001 atgccgaatg ggcggcagac ggcgcggcgg cgaaagcaat gtggagtacg atgcttgcag
     3061 caaacggcta cacggcgaat gaaaaagccc gcgttaaaat cgttacgcct gagtctttgt
     3121 ccgtacacgg tatgtacaaa gtgtccgata aagccgacct gttcggcgac gtaacttgga
     3181 cgcgccacag ccgcttcgat aaggcggaac tggtttttga aaaagaaaaa accatcgtca
     3241 acggcaaatc cgaccgcacc accatcaccc ccaactggcg caacacctac aaagtcggct
     3301 tcggcggttc ttatcaaatc agcgaaccgc tgcaactgcg cgccggcatc gcttttgaca
     3361 aatcgcccgt ccgcaacgcc gactaccgca tgaacagcct gcccgacggc aaccgcatct
     3421 ggttctccgc cggcatgaaa taccatatcg gcaaaaacca cgtcgtcgat gccgcctaca
     3481 cccacatcca catcaacgac accagctacc gcacggcgaa ggcaagcggc aacgatgtgg
     3541 acagcaaagg cgcgtcttcc gcacgtttca aaaaccacgc cgacatcatc ggcctgcaat
     3601 acacctacaa attcaaataa acggcccgcc gtttgattgt agaaatgccg tctgaaacag
     3661 atttccgttt cagacggcat tgttttaggc gcgatttata gtggattaac aaaaatcag
//
LOCUS       DAWEWP010000174         3711 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWEWP010000174 DAWEWP010000000
VERSION     DAWEWP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.174"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(99..1016)
                     /gene="thrB"
                     /locus_tag="WGN53_001836"
     CDS             complement(99..1016)
                     /gene="thrB"
                     /locus_tag="WGN53_001836"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ0140785.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLVAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1053..1781)
                     /gene="ubiG"
                     /locus_tag="WGN53_001837"
     CDS             complement(1053..1781)
                     /gene="ubiG"
                     /locus_tag="WGN53_001837"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ0140786.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1933..3174
                     /locus_tag="WGN53_001838"
     CDS             1933..3174
                     /locus_tag="WGN53_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ0140787.1"
                     /translation="MPTKTPSLFGCAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGNALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWMGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3232..>3711
                     /locus_tag="WGN53_001839"
     CDS             3232..>3711
                     /locus_tag="WGN53_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0140788.1"
                     /translation="MELKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQHRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMRRELVGLYGVPPERIQ
                     "
BASE COUNT          880 a         1176 c          855 g          800 t
ORIGIN      
        1 gaaaataccc ggctatcgtc tcattttcgt tttaatcagc cataaaaatg ccgtctgaaa
       61 ccctttcaga cggcatttct gtcaaacgcc ggacgcactc aatccaaact caacagcagg
      121 ttgcggaacg cgttcgggtc tttgataaac gtcatctcgc ccgcctgcgg aaaatgaaaa
      181 tccaacaggc gcgacaccca aaaacggatg cagccggcgc gttgggcggt cggaaaatac
      241 gccttttctt cggcactcaa ggggcgcacg ccctcataac cgccgataaa cgcctttttc
      301 aacgcctcat ccaacttatt gtccgccgtc cttgcccaat cgttgaccgc aatcgccaag
      361 tcatacataa aattgccccg gcaggcataa taaaaatcga taaagcccga cacctgaccg
      421 ccgtcaagca acacattgtc tttaaacaaa tcggcgtgga tgatgcccga aggcagatga
      481 ttgccgagat tgtccttcag cgcatcgatt tcggaacaca gcagcgcagc atcgtcttgc
      541 gacaggacgg gcagcagccg ggcgcacgcc tccgtccacc acgcatcgta acgcgggttt
      601 tccatttcca aagggaaatc ggcggcgaca aggtgcattt tggccaacat cgcaccggta
      661 tggaaacact gctccgccgt cggcagcgca gtatccgaac ctttcaggca ggcaaccaag
      721 caggcgggct tgcccgccaa aacggaatca agccggccgt ctttgcgcgc aaccggcgcg
      781 gcaaccgcca cgcccttcat actcaaatgc cggttaagct ccagaaaaaa cggcagctct
      841 tcctgtttca acacttcaaa cacggtcagc acataacgtc ccgaagtcgt cgtcagaaaa
      901 taattgctgt tggtaatccc ctgcgcgatg ccctgcaggg aaacaaattc ccccaaatcg
      961 taaccgctca ggaagccgcg catttcatca tcggaaacac tggtatagac agacatatca
     1021 actcacttgc tcaaaaacgg gcaaacccgc cgtcagaacg ccggacggca ggcaaacata
     1081 taattcacat cggtcgaatc gcacagggca taagtttgcg acaacacatg gtaagtcata
     1141 cccttcgtat ccgccacatc caagcctgcc tgacggcaca ttcgcgccag ctcggcaggt
     1201 gcgatgaatt ttttccagtc gtgcgtgcct ttggggacaa acttcaacag atattccgcc
     1261 gccacaatca gatgcaggta cgatttcggg tttttattga tggtagaaaa aaacaccatg
     1321 ccgtccggtt tgaccagatt ggcacaagca cgcacgatgg cggcgggatc ggggacgtgt
     1381 tccatcattt ccatgcacgt taccacatcg aacgagtgcg gttccgcctc ggcaaggtct
     1441 tccacgcgga tacattcgta ttcgatatcg gcgacattgt tcaaagccgc gtgcaggcgg
     1501 gcggtttcca gcgactgctc cgccatgtcg atacccttta caaacgccgc gccgcgccgc
     1561 gccatgcttt ccgccaggat gccgccgccg cagcccacat ccaaaacccg tttgccgcac
     1621 aaatccgcgt gtccgtcgat ataatccagc cgcagcggat tgatgtcgtg caaggttttg
     1681 aactcgcccg acttgtccca ccatttgtcg gcaatccggc tgaatttggc gatttccccc
     1741 tcatcgacat tatatttttt gtcggacatt ttccctccca tctgacgaac cgcccactcc
     1801 aaaacccaag attatactat ggaacgcaat gccgtctgaa agccttttcg ggcggcgcgg
     1861 cgcaaatacc ttacaaatcc ttacacttta cggcataatg gcggctcgct ttttctggca
     1921 gaaagacaaa atatgcccac caaaacccct tcactgttcg gctgcgcgat gattatcgcc
     1981 ggcacggtca tcggcgcagg tatgctcgcc aacccgaccg ccacatccgg cgtatggttt
     2041 accggctcgc tggccgtgtt gctgtacacc tggttttcca tgctctccag cggcctgatg
     2101 attttggaag tcaacaccca ctacccccac ggcgcgagct tcgacaccat ggttaaagac
     2161 ctgctcggac gcggctggaa catcatcaac ggtatcgccg tcgccttcgt tttatacctg
     2221 cttacttacg cttatatctt cgtcggcggc gacctgaccg ccaaaggcat cggcaacgcc
     2281 ttgggcggca atgtttcgct taccgtcgga caactcgtct tcttcggcat cctcgccttt
     2341 tgcgtatggg catccgcacg cttggtcgac cgattcacca gcgtcctcat cggcggcatg
     2401 gtattaacct ttatttgggc aaccggcggc ctgattgccg atgccaaaat gcctgttttg
     2461 tttgacaccc aagcccctgc cggcaccggc tactggattt acgccgccac cgccctgccc
     2521 gtctgcctcg cttccttcgg cttccacggc aacgtttcca gcctgctcaa atactttaaa
     2581 ggcgacgcgc ccaaagtggc taaatccatc tggatgggca cactgattgc gctggtaatt
     2641 tacgtcctct ggcaaaccgc catccaaggc aacctgccgc gcaacgagtt cgcccccgtg
     2701 attgccgccg aagggcaagt ctccgtcctc atcgaaaccc tgtccaaatt cgcccaaacc
     2761 ggcaatatgg acaaaatatt gtccctgttt tcctatatgg cgatcgccac ctcgttttta
     2821 ggcgtaacgc tcggactctt cgactacatc gcagacatct tcaaatggaa cgacagcgtg
     2881 tccggccgca ccaaaaccgc cgcgctgacc ttcctgccgc ccctgatttc ctgcctgctc
     2941 ttccccaccg gcttcgttac cgccatcggc tacgtcggcc tggcggcaac cgtctggaca
     3001 ggcatcatcc ccgccatgct gctctaccgt tcgcgcaaaa aattcggcgc aggcaaaacc
     3061 tataaagttt acggcggctt gtggctgatg gtttgggtct tccttttcgg catcgtcaac
     3121 atcgccgcac aggtattgag ccaaatggaa ctcgtccccg tatttaaagg ataaaggcaa
     3181 aatgccgtct gaagccctgt tcagacagcc ttacgacatt cggaaaatca tatggaactc
     3241 aaaatagaca ttgcaaccaa caacttcaaa cacggcggcg gcacggaacg ctacacattg
     3301 gatttggtaa agggtctgaa cagacaaaac atcacaccgg ccgtttatgc gacgaaattt
     3361 gatcacggca ttcctgaata cgccatgatc gaaccccatc ttgtcgatca acaccggacg
     3421 ctgaaaaaac tacgctcatt cctcttttca agtcggctcg ctcaaaccag aaaaaacagt
     3481 gccgccaaac tgattgcctg ccaccacgcc gattacgccg acctcctcat ctgcggcggc
     3541 acacacttgg gctacctgca ccatatggcg caaaaaccga acctgctcga ccgtctcgcc
     3601 atacgccgca accgcagcaa ctacgccacc gccaaactga ttatggcgca ttcccatatg
     3661 atgcggcgcg aactggtcgg actgtacggc gttccccctg aaagaatcca a
//
LOCUS       DAWEWP010000175         3526 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWEWP010000175 DAWEWP010000000
VERSION     DAWEWP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.175"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..302)
                     /locus_tag="WGN53_001840"
     CDS             complement(<1..302)
                     /locus_tag="WGN53_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0140789.1"
                     /translation="MSTFFQQTAQAMIAKHIDCFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPD"
     gene            complement(526..2658)
                     /locus_tag="WGN53_001841"
     CDS             complement(526..2658)
                     /locus_tag="WGN53_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ0140790.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
BASE COUNT          997 a          927 c          824 g          778 t
ORIGIN      
        1 taatcaggga tgctcagttc gtcaaagcgg caaaacaggt tgaagtcgat gcgggtgatg
       61 aggctgtgtt cgagttcggg atcggagagg ctgtgccatt gtccgagcag gacggctttg
      121 aacatggaca acagcgggta ggcaggacgg ccgcggtggt ctctgaggta acgggttctt
      181 tgacggttca ggtattgttc gatcggctgc caatcaatta cctggtccaa cttcaatagt
      241 gggaagcagt cgatgtgctt ggcaatcatg gcttgcgcgg tttgttgaaa gaaggtgctc
      301 atggaaaatc ccctaaatgt cttggtggga atttagggga ttttggggaa ttttgcaaag
      361 gtctctagat gagtgaaaaa gaagtgcagg ctgcctaaaa agacagaaaa agtctttccg
      421 gcagcctgca ctttggtttc atttcagtca gtaaacccag taaacgacgg tctgaaaacg
      481 cagaacgtta cgaaaaaagc agcctacacg cccatccccc gccttctacc cgttctgtaa
      541 atcatacaga tagcggtaat atccgttcgg cttcgccagc aattcctgct gtgttcccgc
      601 ttccacaatc ctgcctttat ccatggcaat gatccggtgt gccgttttaa cagtggacag
      661 acggtgggcg ataatcagca ccgtccggtt ggcgcaaatg gcctgcatgt tctgcataat
      721 cgctcgttca ctttcataat ccagcgcgct ggtggcttca tcaaaaatca gaatgcgcgg
      781 attggtgatt aacgcgcggg caatcgcaat acgctgccgc tgtccgcccg acaagccggc
      841 cccttgttcg cccaccacgg tgccgtagcc ttccggcagc tccataataa actcgtgtgc
      901 gcccgccagt ttggctgctt cgataatgcg ttccagcggc atacccgtat ccgtcagcgc
      961 gatattgtcg cgtatgctgc ggttgagcag cacattctcc tgcaagacca cgccgacctg
     1021 ccgccgcagc caggcaggag cggccaaagc caaatcgttg ccgtccacca acacccgtcc
     1081 ctgctccggt acatacagac gctgcaccaa tttggtgagt gtggatttgc ccgaccccga
     1141 acgtcccaca atccccagca cttcccccgc ccgaatccgc aggttcaaat cctgcaaaat
     1201 cagcctgccg tccgccttat agcggaaatc gacatgttcg aacgtaatct ccccccggat
     1261 atcgggcaaa gccaaatgcg aagacgcatt ctcggtcggc gcattcagaa tatcccccaa
     1321 acgcgccacc gaaatcccca cctgctggaa atcctgccac aactgcgcca aacggataac
     1381 aggcgccgcc acctgtcccg agagcatatt aaacgcaatc agctgcccca ccgtcagctt
     1441 gccctcaatt accagccgtg cgccaatcca caacgtcgcc accgtcacca gcttctgaat
     1501 cagctgcacc ccctgctggc cgaccaccgc caacttcgtt acccgaaatc ccgaagccac
     1561 ataagccgcc aactgattgt cccaacgctg cgtcatctgc ggctccaccg ccatcgcctt
     1621 taccgtaccc accgcagtga tgctttctac taaaaacgac tggttgtctg cattgcgcgc
     1681 gaacttatcg ttcagacgcg tccgcagtat cggactgata aatgccgacc aaaacgcata
     1741 ggcaggcaac gaagccaata ccacccaagt cagagtggag ctgtaatacc acatcaccgc
     1801 cagaaagata aacgaaaacg ccaaatccaa caccgaagtc agcgcctgac cggtcaagaa
     1861 attgcgaatc tgctccaatt cccgcacccg agccaccgta tcacccactc gtctgtgctc
     1921 gaaataggat aaaggcaggg aaagcagatg ccggaacaaa cgcgcgccca attccacatc
     1981 aatacgtgaa gtcgtatgtg caaacagata cgtccgcaaa ccgcccaaca caatctcaaa
     2041 cagcgacacc accaacaaag ccaccgacac cacatccaaa gtagagaatc cccgatgtac
     2101 cagcaccttg tccatcacca cttggaaaaa cagaggcgta atcagcgcaa acagctgcaa
     2161 caccaccgac accaccaata cttcaaaaaa caaccggcgg tatttgatta ccgccggaat
     2221 aaaccaggta aagtcaaact ttgccaaact gcccaatacc gaagcgcggg aagcaaccaa
     2281 tatcagtttg cccgaatatc tgttagaaaa ttcggcaaaa gacaataccg cagacttatt
     2341 cgtaaccaaa tcctgtatca aaaattgggc atgctcaccc tcaccgtctg ttttggccaa
     2401 aatgaaatgg ttgccgtcat cacaccatac caatgcgggt aaagtcgcca tagccaaacg
     2461 tttaataggc tggcggacta cctttgcctt caatcccaaa gatttggcgg ctaacagcca
     2521 ttgcgtttca tttaaatcgc tctgtgcgga agtacaaaat tcatgctgta tatcggcagg
     2581 attggcggca atgccgtggt aatgggcgag gatgatgagg gcggaaaggg cggggagcgg
     2641 tgcggatacg atagacataa ataaaatata gttagattgg atgtggataa cggctggctg
     2701 gaaaaggaat atattaagta gaaagaaata tataaataaa acagcagaac gcattgtaag
     2761 gatatatatg ggaattgtaa agagaaagta tggaaaagtt ctcgtttcag gaaggtaaaa
     2821 cggcttagga atcgagttag atgaggatgc ctcgcacctc tcgtgcctcc tgcataccgt
     2881 taaggcacag ggttaaggtg caggctgctc cgaactctgt tgcggtcggg taatgttatt
     2941 ttttgtgttt caggcagcct gaaatatctg tatatttttg ttttaaatag attttaaaga
     3001 ttgataactg ttcttgacga tttttcaaga aaggagtaaa tttcaagaaa ggagtaaagt
     3061 gacttattat caatgacaag caacgcgcga agtgacaagg aaaactatct acttaaattc
     3121 taaggaggct tcgaatatca taaaccaatc agaaacatag agataaaaat tatgtacaaa
     3181 tataatcctc ttatacaatt tattgcacag ttgattatgt cttatggagc aagcgtaggg
     3241 tgggcacttg ctgccccacg cgtttcatat ttcaaggcag cctgaaaccg tgtgggcata
     3301 aatgcctacc ctacatccca aaaaacaagc gcagcctgcg tgtgtagggt gcgaactttc
     3361 ggcaggtaga cacgcggttt tatagtttca agctgagttg ggatgcttaa gataaagtac
     3421 aaaaccttaa aaaataaggg ggctgtacta gattagccct aaattccaca ccaatcccgc
     3481 aaaattttta gctgtcggga cggtgtgccg aagttaaacc gaaatt
//
LOCUS       DAWEWP010000176         3497 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWEWP010000176 DAWEWP010000000
VERSION     DAWEWP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.176"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..2846
                     /locus_tag="WGN53_001842"
     rRNA            <1..2846
                     /locus_tag="WGN53_001842"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2943..3056
                     /gene="rrf"
                     /locus_tag="WGN53_001843"
     rRNA            2943..3056
                     /gene="rrf"
                     /locus_tag="WGN53_001843"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            3092..>3497
                     /locus_tag="WGN53_001844"
     CDS             3092..>3497
                     /locus_tag="WGN53_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ0140791.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQAS"
BASE COUNT          931 a          768 c         1046 g          752 t
ORIGIN      
        1 cgacgaagga cgtgtaagcc tgcgaaaagc gcgggggagc tggcaataaa gctatgatcc
       61 cgcgatgtcc gaatggggaa acccactgca ttctgtgcag tatcctaagt tgaatacata
      121 gacttagaga agcgaacccg gagaactgaa ccatctaagt acccggagga aaagaaatca
      181 accgagattc cgcaagtagt ggcgagcgaa cgcggaggag cctgtacgta ataactgtcg
      241 agatagaaga acaagctggg aagcttgacc atagtgggtg acagtcccgt attcgaaatc
      301 tcaacagcgg tactaagcgt acgaaaagta gggcgggaca cgtgaaatcc tgtctgaata
      361 tggggggacc atcctccaag gctaaatact catcatcgac cgatagtgaa ccagtaccgt
      421 gagggaaagg cgaaaagaac cccgggaggg gagtgaaaca gaacctgaaa cctgatgcat
      481 acaaacagtg ggagcgccct agaggtgtga ctgcgtacct tttgtataat gggtcaacga
      541 cttacattca gtagcgagct taaccgaata ggggaggcgt agggaaaccg agtcttaata
      601 gggcgatgag ttgctgggtg tagacccgaa accgagtgat ctatccatgg ccaggttgaa
      661 ggtgccgtaa caggtactgg aggaccgaac ccacgcatgt tgcaaaatgc ggggatgagc
      721 tgtggatagg ggtgaaaggc taaacaaact cggagatagc tggttctccc cgaaaactat
      781 ttaggtagtg cctcgagcaa gacactgatg ggggtaaagc actgttatgg ctagggggtt
      841 attgcaactt accaacccat ggcaaactca gaataccatc aagtggttcc tcgggagaca
      901 gacagcgggt gctaacgtcc gttgtcaaga gggaaacaac ccagaccgcc agctaaggtc
      961 cccaatgata gattaagtgg taaacgaagt gggaaggccc agacagccag gatgttggct
     1021 tagaagcagc catcatttaa agaaagcgta atagctcact ggtcgagtcg tcctgcgcgg
     1081 aagatgtaac ggggctcaaa tctataaccg aagctgcgga tgccggttta tcggcatggt
     1141 aggggagcgt tctgtaggcc gatgaaggtg cattgtaaag tgtgctggag gtatcagaag
     1201 tgcgaatgtt gacatgagta gcgataaagc gggtgaaaag cccgctcgcc gaaagcccaa
     1261 ggtttcctgc gcaacgttca tcggcgtagg gtgagtcggc ccctaaggcg aggcagaaat
     1321 gcgtagtcga tgggaaacag gttaatattc ctgtacttga ttcaaatgcg atgtggggac
     1381 ggagaaggtt aggttggcaa gctgttggaa tagcttgttt aagccggtag gtggaagact
     1441 taggcaaatc cgggtcttct taacaccgag aagtgacgac gagtgtctac ggacacgaag
     1501 caaccgatac cacgcttcca ggaaaagcca ctaagcttca gtttgaatcg aaccgtaccg
     1561 caaaccgaca caggtgggca ggatgagaat tctaaggcgc ttgagagaac tcaggagaag
     1621 gaactcggca aattgatacc gtaacttcgg gagaaggtat gccctctaag gttaaggact
     1681 tgctccgtaa gccccggagg gtcgcagaga ataggtggct gcgactgttt attaaaaaca
     1741 cagcactctg ctaacacgaa agtggacgta tagggtgtga cgcctgcccg gtgctggaag
     1801 gttaattgaa gatgtgagag catcggatcg aagccccagt aaacggcggc cgtaactata
     1861 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aatggcgtaa
     1921 cgatggccac actgtctcct cctgagactc agcgaagttg aagtggttgt gaagatgcaa
     1981 tctacccgct gctagacgga aagaccccgt gaacctttac tgtagctttg cattggactt
     2041 tgaagtcact tgtgtaggat aggtgggagg cttagaagca gagacgccag tctctgcgga
     2101 gccgtccttg aaataccacc ctggtgtctt tgaggttcta acccagaccc gtcatccggg
     2161 tcggggaccg tgcatggtag gcagtttgac tggggcggtc tcctcccaaa gcgtaacgga
     2221 ggagttcgaa ggttacctag gtccggtcgg aaatcggact gatagtgcaa tggcaaaagg
     2281 tagcttaact gcgagaccga caagtcgagc aggtgcgaaa gcaggacata gtgatccggt
     2341 ggttctgtat ggaagggcca tcgctcaacg gataaaaggt actccgggga taacaggctg
     2401 attccgccca agagttcata tcgacggcgg agtttggcac ctcgatgtcg gctcatcaca
     2461 tcctggggct gtagtcggtc ccaagggtat ggctgttcgc catttaaagt ggtacgtgag
     2521 ctgggtttaa aacgtcgtga gacagtttgg tccctatctg cagtgggcgt tggaagtttg
     2581 acgggggctg ctcctagtac gagaggaccg gagtggacga acctctggtg taccggttgt
     2641 aacgccagtt gcatagccgg gtagctaagt tcggaagaga taagcgctga aagcatctaa
     2701 gcgcgaaact cgcctgaaga tgagacttcc cttgcggttt aaccgcacta aagagtcgtt
     2761 cgagaccagg acgttgatag gtggggtgtg gaagcgcggt aacgcgtgaa gctaacccat
     2821 actaattgct cgtgaggctt gactctatca tttgaagaac ttcaagagat aaaagcttac
     2881 tgactgattc agtcattacc gaatatattg attaaggctt taccgatttg taacagttta
     2941 agtttggcgg ccatagcgag ttggtcccac gccttcccat cccgaacagg accgtgaaac
     3001 gactcagcgc cgatgatagt gtggttcttc catgcgaaag taggtcactg ccaaacaccc
     3061 tttcagaaaa cccccgatta ttcgggggtt tttgctttgc ccggaaaaaa tgtcggggat
     3121 ggcgggacgg catccgtacg gcgtccggtc gggtttgcgg aggaacggct tgaaactttg
     3181 ggatattcat tttagaatga cccgttttat cgtcgcaaga tgcggtttat tgtttgcaac
     3241 ccttaaagga aaaaccatga agaaaatgtt cgtgctgttc tgtatgctgt tctcctgcgc
     3301 cttctccctt gcggcggtaa acatcaatgc ggcttcgcag caggagttgg aggcgctgcc
     3361 gggcataggc cctgcgaagg cgaaggccat tgcggaatac cgtgcgcaaa acggtgcgtt
     3421 caagtctgtg gacgatttga ccaaggtaaa gggcatcggc cctgcggtgc tggcgaagct
     3481 gaaggatcag gcttctg
//
LOCUS       DAWEWP010000177         3427 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWEWP010000177 DAWEWP010000000
VERSION     DAWEWP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.177"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            314..3079
                     /locus_tag="WGN53_001845"
     CDS             314..3079
                     /locus_tag="WGN53_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0140792.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQQYFVQTGGLKFNSNSGKWER
                     DLQRQFWKTSYYKQYKDPQKLKKYIEDQDKDWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
     gene            3122..3364
                     /locus_tag="WGN53_001846"
     CDS             3122..3364
                     /locus_tag="WGN53_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140793.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESRHSMLRQFIEND"
BASE COUNT          974 a          846 c          828 g          779 t
ORIGIN      
        1 gcctgcgcgg gaatgacggc tgcagatgcc cgacggtctt tatagcggat taataaaaat
       61 caggacaagg cggcgagcca cagacagtac aaacagtacg gaaccgattc acttggtgct
      121 tcagcacctt agagaatcgt tctctttgag ctaaagcgag acaacgctgt actggttttt
      181 gttaatccac tataaatatc caattgaaat cttcagacgg tatatcaaat ttacactttt
      241 ttaatgttta tgccgcctga aaaaaatgct agtatatttc ctaattgtct gactgtttat
      301 tgttgaggaa aatatgagat cttctttccg gttgaagccg atttgttttt accttatggg
      361 tgttatgcta tatcatcata gttatgccga agatgcaggg cgcgcgggca gcgaggcgca
      421 gatacaggtt ttggaagatg tgcacgtcaa ggcgaagcgc gtaccgaaag acaaaaaagt
      481 gtttaccgat gcgcgtgccg tatcgacccg tcaggatata ttcaaatcca gcgaaaacct
      541 cgacaacatc gtacgcagca tccccggtgc gtttacacag caagataaaa gctcgggcat
      601 tgtgtctttg aatattcgcg gcgacagcgg gttcgggcgg gtcaatacga tggtggacgg
      661 catcacgcag accttttatt cgacttctac cgatgcgggc agggcaggcg gttcatctca
      721 attcggtgca tctgtcgaca gcaattttat tgccggactg gatgtcgtca aaggcagctt
      781 cagcggctcg gcaggcatca acagccttgc cggttcggcg aatctgcgga ctttaggcgt
      841 ggatgacgtc gttcagggca ataataccta cggcctgctg ctaaaaggtc tgaccggcac
      901 caattcaacc aaaggtaatg cgatggcggc gataggtgcg cgcaaatggc tggaaagcgg
      961 agcatctgtc ggtgtgcttt acgggcacag caggcgcagc gtggcgcaaa attaccgcgt
     1021 gggcggcggc gggcagcaca tcggaaattt tggcgcggaa tatctggaac ggcgcaagca
     1081 gcaatatttt gtacaaacag gcgggttgaa attcaattcc aacagcggaa aatgggaacg
     1141 cgatttgcaa aggcaattct ggaaaaccag ttactataaa caatacaaag acccccagaa
     1201 actgaaaaaa tacatcgaag accaagataa agactggcgg gaaaacctgg cgccgcaata
     1261 cgacatcacc cccatcgatc cgtccagcct gaagcagcag tcggcaggca atctgtttaa
     1321 attggaatac gacggcgtat tcaataaata cacggcgcaa tttcgcgatt taaacaccaa
     1381 aatcggcagc cgcaaaatca tcaaccgcaa ttatcaattc aattacggtt tgtctttgaa
     1441 ctcgtatacc aacctcaatc tgaccgcagc ctacaattcg ggcaggcaga aatatccgaa
     1501 agggtcgaag tttacaggct gggggctttt aaaggatttt gaaacctaca acaacgcaaa
     1561 aatcctcgac ctcaacaaca ccgccacctt ccggctgccc cgcgaaaccg agttgcaaac
     1621 cactttgggc ttcaattatt tccacaacga atacggcaaa aaccgctttc ctgaagaatt
     1681 ggggctgttt ttcgacggtc ctgatcagga caacgggctt tattcctatt tggggcggtt
     1741 taagggcgat aaagggctgc tgccccaaaa atcaaccatt gtccaaccgg ccggcagcca
     1801 atatttcaac acgttctact tcgatgccgc gctcaaaaaa gacatttacc gcttaaacta
     1861 cagcaccaat accgtcggct accgtttcgg cggcgaatat acgggctatt acagctcgga
     1921 aggcgaattt aagcgggcat tcggagaaaa ctcgccgaca tacaagaaac attgcaaccc
     1981 gagctgcggg ctttatgaac ccgtattgaa aaaatacggc aaaaagcgcg ccaacaacca
     2041 ttcggtcagc attagtgcgg acttcggcga ttatttcatg ccgttcgcca gctattcgcg
     2101 cacacaccgt atgcccaaca tccaagaaat gtatttttcc caaatcggcg actccggcgt
     2161 tcacaccgcc ttaaaaccag agcgcgcaaa cacttggcaa tttggcttca atacctataa
     2221 aaaaggattg ttaaaacaag atgatacatt aggattaaaa ctggtcggct accgcagccg
     2281 catcgacaac tacatccaca acgtttacgg gaaatggtgg gatttgaacg gggatattcc
     2341 gagctgggtc agcagcaccg ggcttgccta caccatccaa catcgcaatt tcaaagacaa
     2401 agtgcacaaa cacggttttg agttggagct gaattacgat tatgggcgtt ttttcaccaa
     2461 cctttcttac gcctatcaaa aaagcacgca accgaccaac ttcagcgatg cgagcgaatc
     2521 gcccaacaat gcgtccaaag aagaccaact caaacaaggt tatgggttga gcagggtttc
     2581 cgccctgccg cgagattacg gacgtttgga agtcggtacg cgctggttgg gcaacaaact
     2641 gactttgggc ggcgcgatgc gctatttcgg caagagcatc cgcgcgacgg ctgaagaacg
     2701 ctatatcgac ggcaccaacg ggggaaatac cagcaatttc cggcaactgg gcaagcgttc
     2761 catcaaacaa accgaaactc ttgcccgcca gcctttgatt tttgattttt acgccgctta
     2821 cgagccgaag aaaaacctta ttttccgcgc cgaagtcaaa aatctgttcg acaggcgtta
     2881 tatcgatccg ctcgatgcgg gcaatgatgc ggcaacgcag cgttattaca gctcgttcga
     2941 cccgaaagac aaggacgaag acgtaacgtg taatgctgat ggcttgtgca acggcaaata
     3001 cggcggcaca agcaaaagcg tattgaccaa ttttgcacgc ggacgcacct ttttgatgac
     3061 gatgagctac aagttttaaa ggcagcccgc gttttgtgga aaaccgcaaa aaaaagccaa
     3121 aatgccgtct gaaagccctt cagacggcat ttgtttcccc aaacgcatca tcctgccgca
     3181 agcctatgcc aatccgtttt atcgcatcgg caactcaaag aaaaatccat ttcattccca
     3241 cgcagggaag ccggtttttg atttcggtta tttttggttg tttcgggtaa tttatgagtc
     3301 gtcattcccg caaaagcggg aatctagaca ttcaatgcta aggcaattta tcgaaaatga
     3361 ctgaaactca aaaaactgga ttcccacttt cgtgggaatg acgcggtgca ggtttccgta
     3421 tggatgg
//
LOCUS       DAWEWP010000178         3400 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWEWP010000178 DAWEWP010000000
VERSION     DAWEWP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.178"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(214..1278)
                     /locus_tag="WGN53_001847"
     CDS             complement(214..1278)
                     /locus_tag="WGN53_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0140794.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRENRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIIH
                     NGIDTDRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTENIASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAAH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="WGN53_001848"
     CDS             complement(1279..2037)
                     /locus_tag="WGN53_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0140795.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFQRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YATYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="WGN53_001849"
     tRNA            complement(2112..2187)
                     /locus_tag="WGN53_001849"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..3400)
                     /locus_tag="WGN53_001850"
                     /pseudo
     CDS             complement(2241..3400)
                     /locus_tag="WGN53_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
BASE COUNT          770 a          825 c          903 g          902 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc agtttatatt gcatccaata atttgtcggc
      241 ggtatggttg atggtaaaac gggcggcgac gcattgatga gctgccgttg ccagtgcgtt
      301 ttttgcttcg gggtttaaga cgaggcgttc gatttcgtcc gccaaagatt caggcgtaag
      361 cctatccagc agaatccccg attgatgatg ttcgacaatt tccttttgcg cccccaaagt
      421 attggaaatc accgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caatgttttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatcaggat gtccgtgccc tgccaatttg agtctgattt gaggatattt
      661 ccgatgcaat atcacacagg cttcaattag gttttccaat cctttttccg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaccg
      781 atcggtatcg ataccgttat gaataatccg gtatttttct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tctgtgtttt tctgtatcag
      901 gcggtggtaa aaatcggttt tgttggcgac gacattatgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggttttccc ggataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat tgtgcagccc cgaagcccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccacc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccagatttg tacagataat atagtttgac
     1321 atatttaatc atcgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 aagaaaccct ttgttcagaa tataaatttt gaaaaaaccc caaatcggac ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg agatggaagt
     1501 atatttgttg aacttgttga aatattgttc ccagttgtcg tacgtataat ggtacataaa
     1561 atgtttcaga cggcgtttgg ggtagggggt ttgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgtct ttcttcggca tcaaacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ctcgacaaaa taggtggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgttgga agacttttgc acccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcgtcga taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcccgaatg tggtttgctt cgtttttggc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg attaagacgc agttgcttcc cccgaaggca
     2281 aacgatgagc tggcggcaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gcaggttggg gtcgttctgc ccgtcccaaa gccggggcgg aagttttcct
     2401 tcgggattgc tttgccggtt ggcaatgccc cacgcgaacg cggcttcgat tgcgtccgcc
     2461 gcgcccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcgcggct ttccatattg tcgttgtggt gcgtcccggt gccgtgcaga
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggca
     2641 aggattgcgc cttacgcgtc ggggcggggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattttc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgggaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagcccg aaaaagatgc cgatattggc ggcggcggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacatgcc ggtcagcaac
     3121 aattcgccta catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atgcgtccgt cagtaatgct ttcgtctccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacacgc cccatacttc tgagctgctt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatt ttcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgtgcc aatccactat attttcgcaa gacgcgtttt
//
LOCUS       DAWEWP010000179         3355 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWEWP010000179 DAWEWP010000000
VERSION     DAWEWP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.179"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            187..1062
                     /locus_tag="WGN53_001851"
     CDS             187..1062
                     /locus_tag="WGN53_001851"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ0140796.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADFTLS
                     VVPYYNKPSQEGIYQHFKTIAEATSIPMIIYNVPGRTVVSMTNDTILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARKLNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            1071..2267
                     /gene="bamC"
                     /locus_tag="WGN53_001852"
     CDS             1071..2267
                     /gene="bamC"
                     /locus_tag="WGN53_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ0140797.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTLIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGSLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKNPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2324..3256)
                     /gene="pip"
                     /locus_tag="WGN53_001853"
     CDS             complement(2324..3256)
                     /gene="pip"
                     /locus_tag="WGN53_001853"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ0140798.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIQHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDIFPRLL"
BASE COUNT          881 a         1123 c          744 g          607 t
ORIGIN      
        1 ccgacggcac cggcataaac cggcgaatat aataaaatga tttcaaacag gaaaaatcaa
       61 tttccaccat acctttcggc gcaaacgccc cacatctgca acccgccgga tttcacacct
      121 gcggtaaagt aggctacaat cagaagcatt accgccccct acaccacaac tgataaaagg
      181 ttctgtatgt tacaaggtag cctggttgcc ctgattaccc cgatgaatca agacggcagc
      241 atccattacg aacaactccg cgatttgatc gactggcaca ttgaaaacgg cacggacggc
      301 atcgttgccg tcggcacgac aggcgaatcc gccaccctct ccgtcgaaga acacaccgcc
      361 gtcatcgaag ccgtcgtcaa acacgttgcc aaacgcgtcc ccgtcatcgc cggcacaggg
      421 gcaaacaaca ccgtcgaagc catcgccctt tcccaagccg ccgaaaaagc cggtgcagac
      481 ttcactctct ccgtcgtccc ctattacaac aagccctccc aagaaggcat ttaccaacat
      541 ttcaaaacca tcgccgaagc cacttcgatt ccgatgatta tctacaacgt gcccggccgt
      601 accgtcgtca gcatgactaa cgacactatc ctgcgccttg ccgaaatccc caacatcgtc
      661 ggcgtgaaag aagccagcgg caacatcggc agcaacatcg aactcatcaa ccgcgcgccc
      721 gaaggcttcg tcgtcctttc cggcgacgac cataccgccc tgccgttcat gctgtgcggc
      781 ggacacggcg tgattaccgt agccgccaac gccgcaccca agctctttgc cgatatgtgc
      841 cgcgccgcgc tgcaaggcga cattgccctc gcacgcaagc tcaatgaccg gctgattccg
      901 atttacgaca ccatgttctg cgaacccagc cccgccgcac cgaaatgggc ggtatccgcc
      961 ctgggcagat gcgaaccgca cgtccgcctg ccgctcgttc cgctgacgga aggcggacag
     1021 gcaaaagtcc gcgccgccct gaaagcctca ggacaactct aaatataact atgccgtctg
     1081 aaccgttcgg acggcataac gcaacaaaca ctttaatatc catcacacag gatgacacga
     1141 tgacccttat caaacccgtc attgccgcgc tcgcactcat cgggcttgcc gcctgctccg
     1201 gcagcaaaac cgaacagccc aagctcgact accaaagccg gtcgcaccgc ctgatcaaac
     1261 ttgaagtccc acccgatttg aacaaccccg accaaggcag cctctaccgc ctgcctgccg
     1321 gttcgggagc cgtccgcgcc agcgatttgg aaaaacgccg cacacccgcc gtccaacagc
     1381 ctgccgatgc cgaagtattg aaaagcgtca aaggtgtccg cctcgagcgc gacggcagcc
     1441 aacgttggct tgtcgttgac ggcaaatccc ctgccgaaat ctggccgctt ctgaaagcct
     1501 tttggcagga aaacggcttc gacatcaaat ccgaagaacc cgccatcgga caaatggaaa
     1561 ccgagtgggc ggaaaaccgt gccaaaatcc cccaagacag cttgcgccgc ctattcgaca
     1621 cagtcggttt gggcggcatc tactccaccg gcgagcgcga caaattcatc gtccgtatcg
     1681 aacagggcaa aaacggcgtt tccgacatct tcttcgccca caaagccatg aaagaagtgt
     1741 acggcggcaa agacaaagac acgaccgtat ggcagccctc cccgtccgat cccaacctcg
     1801 aagccgcttt cctgacgcgc tttatgcaat atttgggcgt tgacggacag caggcggaaa
     1861 acgcatcggc aaaaaacccg accctgcccg ccgccaacga aatggcgcgt atcgaaggca
     1921 aaagcctgat tgtctttggc gactacggca gaaactggcg gcgcaccgcg ctcgccctcg
     1981 accgcatcgg gctgaccgtc gtcggtcaaa acaccgaacg ccacgctttc ctggttcaaa
     2041 aagccccgaa cgaaagcaat gcagttaccg aacaaaaacc cggcctgttc aaacgcctgc
     2101 tgggcaaagg caaagcggag aaacctgccg aacagccgga actgattgtc tatgccgagc
     2161 ctgtcgccaa cggctcgcgc atcgtcctgc tcaacaaaga cggcagcgca tatgccggca
     2221 aagacgcatc cgcattattg ggcaaactcc attccgaact gcgttaaacc attcaaaaca
     2281 aaagcagctt ccaaacagaa gctgcttttt tatgcggaac tttttacaac aaccgaggaa
     2341 aaatatcctc aaccgcctga accaacgcat ccgccaaagg cggatcgaac gcacgatgcc
     2401 ctgcctgaac caccctcaat tccgcttccg gaaaggcttt cgacagcgcc cacgcactct
     2461 gcatcggcgt acacaaatca taccgcccct gcacgataat ggtcgggata tgctgtattt
     2521 tgccgatatt gttcaaaatc gccctatcgc cctgcaacca gccgccgttg acaaaataat
     2581 ggttttccaa acgcgcgatt gccagcgagg cataagcatc ttcatccact tcctccggct
     2641 cgaaacggat cagatagctt tcccaatccg cccaagcctt tgcggcagac aggcacactt
     2701 cttcatcttg atgaaacagc aacccgtgat acgcctcaat cagccggttc cgccgatttt
     2761 cagcaatcgg cgcgacaaat ttttgccatt gttccggata aatccggctc acaccgcccg
     2821 cctcgttcag ccacgccgtt tcagacggcc tgcacaaaaa tatcccgcgc aacaccaatc
     2881 cctttacccg ttccggatgg gtttgggcat aagccagcga caaagtactg ccccacgaac
     2941 cgccgaacac cagccatttc ccgataccca gcatttcacg gactttttca atatccgcca
     3001 ccaaatccca agtcgtattg tcttccgcac aagcatacgg gcgcgaacgt ccgcaaccgc
     3061 gctggtcaat aatgacgatg cggaacacat cgggattgaa aaaaccccga cattcaggcg
     3121 aagcccccgc ccccggtccg ccgtgtaaaa aaatgaccgg cacaccgtcg ggattgcccg
     3181 actcttccca ataaatttga tgaatttcag acacctgcaa gtatccgcta tgaaaaggct
     3241 gttttatctc atacatcatc ttctcccata ttgcctctga caatccgaac attcccacac
     3301 gcaacatcac gcacgaaaca caagccgccc cgaaaggcaa attccgaaaa atcgc
//
LOCUS       DAWEWP010000180         3343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWEWP010000180 DAWEWP010000000
VERSION     DAWEWP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.180"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            60..689
                     /locus_tag="WGN53_001854"
     CDS             60..689
                     /locus_tag="WGN53_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ0140799.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPYE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALGLSPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            864..3242
                     /gene="hmbR"
                     /locus_tag="WGN53_001855"
     CDS             864..3242
                     /gene="hmbR"
                     /locus_tag="WGN53_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HEZ0140800.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVFAADEAATETTPVKAEVKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIDIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLPERQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGKRGYPVEGAGSGANIRGSARGIPDPSQHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLLASYWREADDVNRRRNTNLFYEWTPESDRLSMVKADVDYQKTKV
                     SAVNYKGSFPIEDSSTLTRNYNQKDLDEIYNRSMDTRFKRITLRLDSHPLQLGGGRHR
                     LSFKTFASRRDFENLNRDDYYFSGRVVRTTSSIQHPVKTTNYGFSLSDQIQWNDVFSS
                     RAGIRYDHTKMTPQELNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWRVGYDITS
                     GYRVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGTLDANLYQSNYR
                     NFLSEEQKLTTSGDVSCTQMNYYYGMCSNPYSEKLEWQMQNIDKARIRGIELTGRLNV
                     DKVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLT
                     YLGAKKVKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPVKNLTLRAG
                     VYNVFNRKYTTWDSLRGLYSYSTTNSVDRDGKGLDRYRAPSRNYAVSLEWKF"
BASE COUNT          898 a          876 c          828 g          741 t
ORIGIN      
        1 gggcaagtgc ggattttgcc cgccccgcat ccttatccct taccgacaaa ggaaaactta
       61 tgagtgaaac cgaaaatcaa gccctgacgt ttgccaaacg tctgaaggcg gataccacgg
      121 cggttcacga cagcgtggat aacctcgtta tgtctgtcca accgtttgtc agcaaagaaa
      181 actacatcaa atttttgaaa ctccaatccg ttttccacaa ggccgtcgac cacatctata
      241 aagatgccga attaaacaaa gccattcccg agctggaata catggcgcga tacgatgccg
      301 taacgcaaga cttgaaagat ttgggcgaag agccttacaa attcgacaaa gaattgccat
      361 acgaaactgg caacaaagca atcggctggc tttattgcgc cgaaggatcc aatttgggcg
      421 cggcgttttt gttcaaacac gcccaaaaac tcgattacaa cggcgaacac ggcgcgcgcc
      481 acctcgctcc gcatcccgac gggcgcggca aacactggcg cgccttcgtc gagcatctga
      541 acgcattggg cttaagcccc gaagccgaag ccgaagccat ccaaggcgcg cgcgaagcct
      601 ttgcattcta caaagtcgtg ttgcgcgaaa ccttcggctt ggcagccgat gccgaagccc
      661 ccgaaggtat gatgccgcac aggcactaaa aaataatcga accaaataaa caaggtctcg
      721 gcatagctgt ttgcagggac ctttaattac acggcgcggc tttgtttaca tggattactg
      781 tcttattaaa tattaatgat tatcataaaa tctattattc gctaaccgat ggatgaacaa
      841 tccatacatc ttgagttgat aatatgaaac cattacaaat gctccctatc gccgcgctgg
      901 tcggcagtat tttcggcaat ccggtctttg cggcagatga agctgcaact gaaaccacac
      961 ccgttaaggc agaggtaaaa gcagtgcgcg ttaaaggcca gcgcaatgcg cctgcggctg
     1021 tggaacgcgt caaccttaac cgtatcaaac aagaaatgat acgcgacaac aaagacttgg
     1081 tgcgctattc caccgatgtc ggcttgagcg acagcggccg ccatcaaaaa ggctttgctg
     1141 ttcgcggcgt ggaaggcaac cgtgtcggcg tgagcataga cggcgtaaac ctgcctgatt
     1201 ccgaagaaaa ctcgctgtac gcccgttatg gcaacttcaa cagctcgcgt ctgtctatcg
     1261 accccgaact cgtgcgcaac atcgacatcg taaaaggggc ggactctttc aataccggca
     1321 gcggcgcctt gggcggcggt gtgaattacc aaaccctgca aggacgtgac ttactgttgc
     1381 ctgaacggca gttcggcgtg atgatgaaaa acggttacag cacgcgtaac cgtgaatgga
     1441 caaataccct cggtttcggc gtgagcaacg accgcgtgga tgccgctttg ctgtattcgc
     1501 aacggcgcgg ccatgaaact gaaagcgcag gcaagcgtgg ttatccggta gagggtgctg
     1561 gtagcggagc gaatatccgt ggttctgcgc gcggtattcc tgatccgtcc caacacaaat
     1621 accacagctt cttgggtaag attgcttatc aaatcaacga caaccaccgc atcggcgcat
     1681 cgctcaacgg tcagcagggg cataattaca cggttgaaga gtcttacaac ctgcttgctt
     1741 cttattggcg tgaagctgac gatgtcaaca gacggcgtaa caccaacctc ttttacgaat
     1801 ggacgccgga atccgaccgg ttgtctatgg taaaagcgga tgtcgattat caaaaaacca
     1861 aagtatctgc ggtcaactac aaaggttcgt tcccgataga ggattcttcc accttgacac
     1921 gtaactacaa tcaaaaggac ttggatgaaa tctacaaccg cagtatggat acccgcttca
     1981 aacgcattac cctgcgtttg gacagccatc cgttgcaact cggggggggg cgacaccgcc
     2041 tgtcgtttaa aactttcgcc agccgccgtg attttgaaaa cctaaaccgc gacgattatt
     2101 acttcagcgg ccgtgttgtt cgaaccacca gcagtatcca gcatccggtg aaaaccacca
     2161 actacggttt ctcactgtct gaccaaattc aatggaacga cgtgttcagt agccgcgcag
     2221 gtatccgtta cgatcatacc aaaatgacgc ctcaggaatt gaatgccgag tgtcatgctt
     2281 gtgacaaaac accgcctgca gccaacactt ataaaggctg gagcggtttt gtcggcttgg
     2341 cggcgcaact gaatcaggct tggcgtgtcg gttacgacat tacttccggc taccgtgtcc
     2401 ccaatgcgtc cgaagtgtat ttcacttaca accacggttc gggtaattgg ctgcccaatc
     2461 ccaacctgaa agccgagcgc agcaccaccc acaccctctc tctgcaaggc cgcagcgaaa
     2521 aaggtacttt ggatgccaac ctgtatcaaa gcaattaccg caatttcctg tctgaagagc
     2581 agaagctgac caccagcggc gatgtcagct gtactcagat gaattactac tacggtatgt
     2641 gtagcaatcc ttattccgaa aaactggaat ggcagatgca aaatatcgac aaggccagaa
     2701 tccgcggtat cgagctgacg ggccgtctga atgtggacaa agtagcgtct tttgttcctg
     2761 agggctggaa actgttcggc tcgctgggtt atgcgaaaag caaactgtcg ggcgacaaca
     2821 gcctgctgtc cacccagccg ttgaaagtga ttgccggtat cgactatgaa agtccgagcg
     2881 aaaaatgggg cgtgttctcc cgcctgacct atctgggcgc gaaaaaggtc aaagacgcgc
     2941 aatacaccgt ttatgaaaac aagggctggg gtacgccttt gcagaaaaag gtaaaagatt
     3001 acccgtggct gaacaagtcg gcttatgtgt tcgatatgta cggcttctac aaaccggtga
     3061 aaaacctgac tttgcgtgca ggcgtatata atgtgttcaa ccgcaaatac accacttggg
     3121 attccctgcg cggcctgtat agctacagca ccaccaactc ggtcgaccgc gatggcaaag
     3181 gcttagaccg ctaccgcgcc ccaagccgta attacgccgt atcgctggaa tggaagtttt
     3241 aatctggtat tattgaatta atcgccttgt tgaaaattaa agccgtccga attgtgttca
     3301 agaactcatt cggacggttt tttaccgtaa atctgtgtga tgg
//
LOCUS       DAWEWP010000181         3330 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWEWP010000181 DAWEWP010000000
VERSION     DAWEWP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.181"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            238..1197
                     /locus_tag="WGN53_001856"
     CDS             238..1197
                     /locus_tag="WGN53_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140801.1"
                     /translation="MYQNYDIVILSLGNGLLTRFFRNSSKLNLTLPIVITLFPGVIFG
                     DSESIITRLGANILLFNNYYDYQIGNKFKERYNLSCTNILYGYPILRKKHHCSDGNKV
                     YFIDQVKIPDGKKERLLILKKLIALANRYPHKDLVLLLRVHQNDITVHEDKYSYAELA
                     KNMKLPDNFKIERKRTQDALQEMGYCLSFSSTMLFEAESCGIPIGVISDLGVDKKYAN
                     THFKDSGVLVNLDTVDLDSPNTINPTWAELYTNPDSLSSKEFNQLLEVTSTQPPSFDN
                     YFIAVNDVQPVSMVLLRKFKKLIYHPKQFFVDSKLYKLIKFTK"
     gene            1245..2108
                     /gene="galU"
                     /locus_tag="WGN53_001857"
     CDS             1245..2108
                     /gene="galU"
                     /locus_tag="WGN53_001857"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0140802.1"
                     /translation="MQKIRKAVFPVAGMGARFLPATKASAKEMLPIVDKPLIQYAAEE
                     AVAAGCTELIFITGRNKRNIEDHFDKAYELETELEMREKHKLLGYVQKILPDHVSCIF
                     IRQAEALGLGHAVLCARPAVGNEPFAVILADDLIDAPQGALKQMVDIYNQSGNSILGV
                     ETVDPSQTGSYGIVEVEQLKSYQRVTNIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGGEIQLTDGIARLLDHEFVLAHAFEGKRYDCGSKLGYLEATVAYGLKHPELK
                     DDFKELLKQFV"
     gene            2191..3207
                     /gene="galE"
                     /locus_tag="WGN53_001858"
     CDS             2191..3207
                     /gene="galE"
                     /locus_tag="WGN53_001858"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0140803.1"
                     /translation="MNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPRL
                     KTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPIKYYDNNVS
                     GSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERIL
                     TDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAVF
                     GDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRAF
                     EAASGLTIPYEVKPRRAGDLACFYADPAYTKTQTGWETKRDLAQMMEDSWRWVSNNPN
                     GYDD"
BASE COUNT          994 a          622 c          708 g         1006 t
ORIGIN      
        1 aacttaatat ctaatattta aaatacaatg aataaaaaac gagtcctatt aattgctagt
       61 tatgattctt ttttgaatac tggatatgct gttgcaaaag aaattaacta ttcaacaatc
      121 gatatttata tccaccaaac aaaaaaaaat attctttcgg aatcacaatt aagtttaaat
      181 gggctttctt taaaagaaat caattttttt catattgatg attatttcga caaattaatg
      241 taccagaatt atgatattgt tattctatca ttaggtaatg gtttactgac gcgttttttt
      301 cggaatagta gcaaacttaa tttaacatta cctatagtaa ttactctatt tccaggggta
      361 atttttggtg attcagaaag tattataact aggttaggtg ctaatatttt attgttcaat
      421 aattattatg attaccaaat aggcaataag ttcaaagaac gttacaactt atcgtgtaca
      481 aatattttat atggttatcc tatcctacga aaaaaacacc attgctctga tggcaataaa
      541 gtttatttca ttgatcaagt aaaaattcca gatggaaaaa aagaaagatt attaattcta
      601 aaaaaattaa tagcactggc taatcgctat ccacataaag atttagtact attattacgt
      661 gtccatcaaa atgatatcac agtacatgaa gataaatact cttatgctga attagcaaaa
      721 aatatgaaat tgcctgataa ttttaaaata gagagaaaac ggacacaaga tgccttacaa
      781 gaaatgggat attgtttatc attctcttca acaatgttat ttgaagctga aagctgcgga
      841 attcctattg gtgtcattag tgatttaggg gttgacaaga aatatgccaa tacacatttc
      901 aaagatagtg gtgttttggt taatttagat acggtagact tggactctcc aaataccatt
      961 aatccaactt gggctgaact atatactaat ccagatagcc tctcttcaaa agagtttaac
     1021 caattattag aggttacttc tactcaacca cctagttttg ataattattt tattgcagtt
     1081 aatgacgttc agcctgtatc tatggttctg ttacgtaaat ttaaaaagct tatatatcat
     1141 cctaagcaat tctttgttga ttcaaaacta tataaattaa ttaaatttac taaataattt
     1201 ttactatggg ttgccttatt tatttcaaag gagtgacata aaaaatgcaa aaaattcgta
     1261 aagctgtatt ccctgtagct ggcatgggag cccgattctt acctgccacg aaagccagcg
     1321 ctaaagaaat gctgccaatc gtggacaaac cgttaattca atatgctgcg gaagaagccg
     1381 ttgccgcggg ttgtactgaa ttgattttta tcaccggccg taataagcgt aatattgaag
     1441 accacttcga taaagcctat gagttggaaa ctgaactgga aatgcgtgaa aagcataaat
     1501 tgcttggcta tgtgcaaaaa attctgcctg atcatgtcag ctgtattttc attcgtcagg
     1561 cagaagcttt gggcttgggt catgcggtat tatgcgcccg tcctgctgtc ggtaatgagc
     1621 cttttgccgt gattttggcc gatgacttga ttgatgcacc acaaggcgca ctcaagcaga
     1681 tggtagatat ttacaatcaa agcggcaaca gcattttagg ggtagaaaca gtcgatcctt
     1741 cgcaaaccgg ttcatacggc attgtcgaag tggaacagct gaaaagctat cagcgtgtaa
     1801 ccaatattgt ggaaaaacca aagcctgaag atgcgccgtc aaatttagcg gtagtcggcc
     1861 gttatatttt gacaccgcgt atttttgatt tgctgactaa tctacctcgt ggtgcaggtg
     1921 gtgaaatcca gctaactgat ggtattgccc gcctacttga ccatgaattt gtcttggcgc
     1981 atgcgtttga aggtaagcgt tatgattgtg gcagtaagtt gggctatctg gaagccacgg
     2041 tagcttatgg cttgaagcat cccgaattaa aagatgattt caaagaatta ttgaagcaat
     2101 ttgtataatt ttaaggccgt ctgaaaaaga aaaggcatca tgttttttac actttcaggc
     2161 ggccttttaa acataaagaa ggaaaacgtg atgaacattc tcgttaccgg cggcaccggt
     2221 tttatcggct cgcacaccgt tgtttctttg ctgaaaagcg gtcatcaagt cgtgattttg
     2281 gataacctat gcaattccag catcaatatc ctgccacgct tgaaaacgat taccggccaa
     2341 gagattccgt tttatcaagg cgatatccgc gatcgtgaga ttttgcgccg tatttttgcg
     2401 gaaaaccgca ttgattcggt gattcatttt gccggcttga aagcggtggg tgaaagtgtg
     2461 gccgagccga taaaatatta tgataataat gtttccggca gcttggtgtt ggcggaagaa
     2521 atggcgcgtg cgggcgtgtt tagcattgtg ttcagttctt cggcgacggt ttatggcgat
     2581 ccgggcaaag tgccttatac cgaggatatg ccaccgggcg acaccaccag cccttacggc
     2641 gcatcgaaat cgatggttga gcgcattctc actgacattc aaaaagccga tccgcgctgg
     2701 agcatgattt tgttgcgtta tttcaatccg attggcgcgc atgaaagcgg tttgattggc
     2761 gagcagccaa acggtatccc aaataatttg ttgccttata tctgccaagt ggcggcaggc
     2821 aaactgccgc aattagcggt attcggtgat gactacccta cccctgatgg cacgggtatg
     2881 cgcgactata ttcatgtgat ggatttggca gaaggccatg tcgcggctat gcaggcgaaa
     2941 agcaatgtag caggcacgca tttgttgaac ttaggttccg gccgcgcttc ttcggtgttg
     3001 gaaatcatcc gcgcatttga agcagcttcg ggtttgacga ttccgtatga agtcaaaccg
     3061 cgccgtgccg gtgatttggc gtgcttctat gccgaccctg cctacaccaa aacgcagacc
     3121 ggttgggaaa ccaagcgtga tttggcgcag atgatggaag actcatggcg ttgggtcagc
     3181 aacaacccaa atggctacga cgattaagtt gacctgatac aggccgtctg aaagagatgt
     3241 tttcagacgg cctctttatc tgaaaaacac acattctgtc tgctataatc tgtttatatt
     3301 ttttggctat cctctgaaat ttatgagaaa
//
LOCUS       DAWEWP010000182         3288 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWEWP010000182 DAWEWP010000000
VERSION     DAWEWP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.182"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(96..320)
                     /locus_tag="WGN53_001859"
     CDS             complement(96..320)
                     /locus_tag="WGN53_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140804.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLIIESEKNGRY"
     gene            423..2573
                     /gene="dinG"
                     /locus_tag="WGN53_001860"
     CDS             423..2573
                     /gene="dinG"
                     /locus_tag="WGN53_001860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ0140805.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQNNAQQNLLGFEAPEVLWD
                     SKPKPEELKLLHDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSSGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAVAVRSLLKHVIGLNDALS
                     AARREKEQDGALLDRLTGDFGIFIARIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPGEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRVKTQRYGQQLLAGLPPFKRIG"
     gene            2609..3223
                     /locus_tag="WGN53_001861"
     CDS             2609..3223
                     /locus_tag="WGN53_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ0140806.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGAPVAGAASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIATAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT          824 a         1081 c          807 g          576 t
ORIGIN      
        1 gcacatcctg tcgccggtat tccctaagcg gtacggcaac ctaaaacaaa tgccgtctga
       61 acttccgttt cagacggcat ttttatacgg actactcaat atcttccgtt tttttcactt
      121 tcaataatca gatatgccca aatcaccgcc acccaagccg acaccgttcc catcgccggc
      181 aaaaaatgct gccaatccgt catcggcggc acagggcaac ggccgcattc gatttccgcg
      241 ccgcccaaca accggtacag caacatatcc gcaatccaaa gcaacaacgg gtatttcaaa
      301 cgctgcaacc aaaaaaccaa ccacgtccgc acgattcacc tcacccccca ttccgacaac
      361 gatattgtac cgcttccgtg caatgttaaa ataagcccac ttctaccgcc gtacaaaacg
      421 ccatgctcac cgatttagaa aaaaacgcca tccgcgacca ttaccaaaac atcggcaaaa
      481 acctgcccgg tttccgtccg cgcgcttcgc agcgggaaat gattgcggcg gttgccaacg
      541 ctttttcgcg gacgttggcg cgcgaagaag gcggcgagcc gcccaagcgc gagggcgaga
      601 gcatcgccgt aatcgaaggg ccgaccggcg tgggcaaatc gcttgcctac ctgctggcgg
      661 gcggcatcat ggcgcagacg cgcggcaagc ggctgattgt gagcagcgca acggttgcct
      721 tacaggagca gttggttgac cgcgacctgc cgtttctggt cgaaaaaagc ggtttggaac
      781 tgaccttcgc gcttgccaaa gggcgcggcc gctatctctg cccttacaaa ctctaccaac
      841 tgacgcaaaa caacgcccag caaaacctgc ttggttttga agcaccggaa gtattgtggg
      901 acagcaaacc caagcccgaa gaattgaagc tgctgcacga cattgccgac gaattttccg
      961 cccgacggtt caacggcgac cgcgacactt ggccggaaaa aatcgatgac gcgatttggc
     1021 tcaaagttac caacgaccgc cacggctgcc tgaaaaccgc ctgtcccaac cgtccggaat
     1081 gtccgtttta cctggcacgc gatgtcttgg aaaccgtcga tgtcgtcgtt gccaaccacg
     1141 accttctgct tgccgacatc agtatgggcg gcggcgtgat tctgcccgcg cccgaaaaca
     1201 gtttctattg catagacgaa gcgcaccacc tgcccaaaaa agccctcagc cgttttgccg
     1261 ccgaacattc gtggaatatt gccgtttgga cgctggaaaa actgccgcag ctgaccggca
     1321 agattgccgc gcttaccgat aaagccgaac ttgccaacct tgccgatgaa gccgccgcat
     1381 ccttgctcga cagcctgcac gaatggcagt tccatttggc ggaagagccg tctttgagct
     1441 cggggccgtc tgaaaacgac aggcgaacca acagcgaacc gacttggctg tgggaagacg
     1501 gcaaaatccc cgaaggcctc gaaaccaccg tttccaacac cgccgttgct gtgcgcagcc
     1561 tgctcaaaca cgttatcggg ctgaacgatg cgctttctgc cgcacgccgc gaaaaagaac
     1621 aggacggcgc gctcctcgac cgcctgaccg gcgatttcgg catcttcatc gcccgtatcg
     1681 aacaaatcag cgcggtttgg gatttgctct ccaccgtccc cctcgaaggc gaagagccgc
     1741 tggcaaaatg gataacccgc cgcgccgacg acaaaaacga ctacattttc aacgccagcc
     1801 ccatcagcag cgcgtcccac ctcgccaaca gcctgtggcg gcgcgcggca ggcgcggtat
     1861 tgacctccgc caccctgcaa tccttgggca actttaacct gatgctgcgc caaaccgggc
     1921 tgcaatggct gcccgagacc accaccctcg ccctcaaaag cccctttgac tttgaaaaac
     1981 agggcgaact ctacatcccc cccatatacg ccagccccaa agaccccgaa gcccacaccg
     2041 ccgccatcat cgagtggctg cccaagctca tttcgcccgg cgaagccatc ggcacgctcg
     2101 tcttgttttc ctcgcgcaaa caaatgcagg atgtcgccct gcgcctgccc ggagactacc
     2161 tgccgctctt gctcgtacaa ggcgaattac ccaaagccgt tctcctgcaa aaacaccacc
     2221 gggccataga agaaggcaaa gccagcatca tcttcggact cgacagcttt gccgaaggac
     2281 tcgacctgcc cggcaccgcc tgcgtgcaag tcatcatcgc caaacttccc ttcgccatgc
     2341 ccgacaaccc catcgaaaaa acccaaaacc gctggataga acagcgcggc ggcaaccctt
     2401 tcatcgaaat caccgtcccc gaagccggca tcaaactcat ccaggccgtc ggccgcctca
     2461 tccgcaccga acaagactac ggccgcgtaa ccatcctcga caaccgcgtc aaaacacagc
     2521 ggtacggcca acaattatta gccggcctgc caccgtttaa aaggataggg taaacctacc
     2581 gccctgcccg caacagaaag gaaaaatcat gaacttcacc cgactgctca accaagtctt
     2641 aagcacggtt caaaaaaaag gcaacacctt ctccgacagc ccgctcaatt cattcggcgg
     2701 aggcgcgccg gttgccggtg ccgcgtccat gctgctgaac ggtaaaaacc gcaaaaccat
     2761 taccaaaatc ggttcgaccg ccgctttggg ctacctcgcc taccggggct atcagatgtg
     2821 gcagcaaaac aaagggcggg caaccgtaac acaaagcgat ttccaacctg ccggagaaac
     2881 tgaagaaaca tacagccgca ccgtattgcg caccatgata gccgccgccg cttcagacgg
     2941 catgatagac gaagccgaac gccggactat cgaacaggaa agcggcacag accccgaaac
     3001 tgccgcatgg ctcgccgccg aataccgcct gcccgcaagc atcggggaca tcgccaccgc
     3061 cgtcggcaac gatgaggcgt tggcggcgga ggcctatctg gcggcaaggt tggtctgtgc
     3121 cgatttgtcg cggaaagaaa ccgtcttcct cgcccgcctg tcgcaggctt tgaaactgga
     3181 tgacaatctg gtggagagtt tggaaaggca attggggttt tgatgacaca gccagggagg
     3241 gaaaatcatc aaaaaacggt tattccgacc ccggcctcaa aacggcaa
//
LOCUS       DAWEWP010000183         3263 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWEWP010000183 DAWEWP010000000
VERSION     DAWEWP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.183"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(150..296)
                     /locus_tag="WGN53_001862"
     CDS             complement(150..296)
                     /locus_tag="WGN53_001862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140807.1"
                     /translation="MMRNGGNLSGITENERKSGQGGEAADSTDRTEPIHLVLEHLRES
                     FSLS"
     gene            1058..3199
                     /locus_tag="WGN53_001863"
     CDS             1058..3199
                     /locus_tag="WGN53_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0140808.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNAKVVLDTITVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNDNGGKT
                     VPYSALDKRSYLAKIGTSFGDDDHRIVLSHMKDQHRGIRTVREEFAVSEKNSRITIKR
                     QAPAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSQFKIEDKTGATPAEIQK
                     NRADEATAHDYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHDGKTVSSS
                     NLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARNT
                     EIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIKNHGYELGASY
                     RTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFKNPNLEIGW
                     RGRYVQKATGSILAAGQKNRDKQLENVVRKGFGVNDVFANWKPLGKDTLNVNLSVNNV
                     FNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          870 a          922 c          745 g          726 t
ORIGIN      
        1 caaaagtggg aatctagaaa tgaaaagcag caggaattta tcggaaatga ccgaaactga
       61 acggactgga ttcccgcttt cgcgggaatg acggcgacag ggttgctgtt atagtggatg
      121 aacaaaaacc agtacgtcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
      181 tcaagcacca agtgaatcgg ttccgtccta tccgtactgt ctgcggcttc gccgccttgt
      241 cctgatttcc gttcgttttc ggttattccc gataaattac cgccgtttct catcatttct
      301 ttaacccttc gtcattcccg cgcaggcggg aatctagttt ttttgagttc cagttgtttc
      361 tgataaattc ttgcagcttt gagttcctag attcccactt ttgcgggaag gacgcggaaa
      421 ggttgctttt cgtcattccc gcgcaggctt cgtcattccc gcgcaggcgg gaatccagtc
      481 tgttcggttt cggttttttc cgatagattc ctgccgcgtt aggggttcta gattcccgct
      541 ttcgcgggaa ggacgcggaa aggttgcttt tcgtcattcc cgcgcaggcc tcgtcattcc
      601 cgcacaggct tcgtcattcc cgcgcaggcg ggaatccagt ctgttcggtt tcagtcattt
      661 ccgatagatt cctgccgcgt taggggttct agattcccgc tttcgcggga atgacgcgga
      721 aaggttgctt ttcgtcattc ccgcatttgt taatccacta tatttccgcc gttttttata
      781 gtggactaac aaaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtctg
      841 cggcttcgtc gccttatcct gatttttgtt agtccactat acatttccga caaaacctgt
      901 caacaaaaaa caacacttcg caaataaaaa cgataatcag ctttacacaa atcccccctc
      961 tcgttaatat aaacaaaaat aattattatt tttcttatcc tgccaaacct taacggtttg
     1021 gatttacttc ccttcataca ctcaaaagga cgaacaaatg aacgccccgt ttttccgcct
     1081 cagcctgctc tcgcttaccc tggcggcagg ttttgcccat gcggcagaaa ataatgccaa
     1141 ggtcgtactg gataccatta ccgtaaaagg cgaccgccaa ggcagcaaaa tccgtaccaa
     1201 catcgttacg cttcaacaaa aagacgaaag caccgcaacc gatatgcgcg aactcttaaa
     1261 agaagagccc tccatcgatt tcggcggcgg caacggcacg tcccaattcc tgacgctgcg
     1321 cggcatgggt cagaactctg tcgacatcaa ggtggacaac gcctattccg acagccaaat
     1381 cctttaccac caaggcagat ttattgtcga tcccgctttg gttaaagtcg tttccgtaca
     1441 aaaaggcgcg ggttccgcct ctgccggtat cggcgcgacc aacggcgcga ttatcgccaa
     1501 aaccgtcgat gcccaagacc tgctcaaagg cttggataaa aactggggcg tgcgcctcaa
     1561 cagcggcttt gccagcaacg aaggcgtaag ctacggcgca agcgtattcg gaaaagaggg
     1621 caacttcgac ggtttgttct cttacaaccg caacgatgaa aaagattacg aagccggcaa
     1681 aggtttccgc aatgacaacg gcggcaaaac cgtaccgtac agcgcgctgg acaaacgcag
     1741 ctacctcgcc aaaatcggaa caagcttcgg cgacgacgac caccgcatcg tattgagcca
     1801 catgaaagac caacaccgag gcatccgcac tgtgcgtgaa gagtttgccg tcagcgaaaa
     1861 aaattcacgg ataactatta aacgccaagc ccctgcttac cgcgaaacca ctcaatccaa
     1921 caccaacttg gcgtacaccg gcaaagattt gggctttgtc gaaaaactgg atgccaacgc
     1981 ctatgtgttg gaaaagaaac gctattccgc cgatgacaaa gataacggct acgcaggcaa
     2041 tgtaaaaggc cctaaccata cccgaatcac cactcgaggt atgaatttca acttcgacag
     2101 ccgccttgcc gaacaaaccc tgttgaaata cggtatcaac taccgccatc aggaaatcaa
     2161 accgcaagcg tttttgaatt cgcaatttaa aattgaagat aaaactggtg caaccccggc
     2221 agaaatacaa aaaaaccgtg cagatgaagc cactgctcac gactacaaac tgtccaaccc
     2281 aaccaaaacc gataccggcg cgtatatcga agccattcac gagattgacg gctttaccct
     2341 gaccggcggg ctgcgttacg accgcttcaa ggtgaaaacc catgacggca aaaccgtttc
     2401 aagcagcaac cttaacccga gtttcggtgt gatttggcag ccgcacgaac actggagctt
     2461 cagcgcgagc cacaactacg ccagccgcag cccgcgcctg tatgacgcgc tgcaaaccca
     2521 cggtaaacgc ggcatcatct cgattgccga cggcacgaaa gccgaacgcg cgcgcaatac
     2581 cgaaatcggc ttcaactaca acgacggcac gtttgccgcc aacggcagct acttctggca
     2641 gaccatcaaa gacgcgcttg ccaatccgca aaaccgccac gactctgtcg ccgtccgtga
     2701 agccgtcaac gccggctaca tcaaaaacca cggttacgaa ttgggcgcgt cttaccgcac
     2761 cggcggtctg actgccaaag tcggcgtaag ccacagcaaa ccgcgctttt acgatacgca
     2821 caaagacaag ctgttgagcg cgaatcctga atttggcgca caagtcggcc gcacttggac
     2881 agcctccctt gcctaccgct tcaaaaaccc gaatctggaa atcggctggc gcggccgtta
     2941 tgttcaaaaa gctacgggtt cgatattggc agcaggtcaa aaaaaccgcg acaaacaatt
     3001 ggaaaacgtt gtacgcaaag gtttcggtgt gaacgatgtc ttcgccaact ggaaaccgct
     3061 gggcaaagac acgctcaatg tcaatctttc agtcaacaac gtgtttaaca cgttctacta
     3121 tccgcacagc caacgctgga ccaataccct gccgggcgtg ggacgtgatg tacgcttggg
     3181 cgtgaactac aagttctaaa acgcacatcc cgaaaaaatg ccgtctgaaa gcctttcaga
     3241 cggcatctgt tctgataatt tga
//
LOCUS       DAWEWP010000184         3244 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWEWP010000184 DAWEWP010000000
VERSION     DAWEWP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.184"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..1490)
                     /locus_tag="WGN53_001864"
     CDS             complement(<1..1490)
                     /locus_tag="WGN53_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0140809.1"
                     /translation="MTSANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSIGRDNKITNTASLLASLDNIFLNLRNVSRDIRE
                     TGKFKPNDIQQAIGDIFIAAGDGLQYIKQQTEAMAQSKFLPTKLKTGLNDVLNSRMLK
                     SSTVLQHELNYLGFKIKDYGNERLGESIMNIDDFTPSKIANFFADPDTYSNVLEEVSR
                     FIYSLVPDDANPWKGGEDYIGRGISEWGELLEKWYKQDFLPYLEKEWDQFPKFEDWLP
                     EFPEWAREWLKLDPKRSGKYHVYDPLALDLDGDGIETVAAKGFAGALFDHRNQGIRTA
                     TGWVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNII
                     NAADAAFQTLRVWQDLNQDGISQANELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQ
                     GSYTKTDGTTAKMGDLLLAADNLHSRFKDKVELTAEQAKAANLAGIG"
     gene            complement(2280..2873)
                     /locus_tag="WGN53_001865"
     CDS             complement(2280..2873)
                     /locus_tag="WGN53_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEZ0140810.1"
                     /translation="MDGDGIETVATKGFAGALFDHRNQGIRTATGWVSADDGLLVRDL
                     NGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQSLRVWQD
                     LNQDGISQANELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKMGD
                     LLLAADNLHSRFTNKMLSISHVRENTISPFVLGCLNK"
     gene            complement(2764..3244)
                     /locus_tag="WGN53_001866"
                     /pseudo
     CDS             complement(2764..3244)
                     /locus_tag="WGN53_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248708.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          944 a          654 c          597 g         1049 t
ORIGIN      
        1 ccgatgcccg caagattggc ggcttttgcc tgttcggcag tgagttccac tttgtctttg
       61 aagcggctgt gcagattgtc ggctgctaaa agtaaatccc ccatttttgc ggttgtaccg
      121 tctgttttgg tatagctgcc ttgctgagcc aaagtgttac cgttaccgag atttttattt
      181 acatctttat aggcgagatc caaagattgg atacccaatt cttcaagggt acgcaattca
      241 ttagcttggg aaatgccgtc ctggttgaga tcctgccata cacgcagggt ttggaatgcg
      301 gcgtctgccg cgttgatgat gttgtcgccg tttgaatcca attcggccaa agctgcatag
      361 ccgtgtttgg caaaagaacc gtctgccagt ttggtgttgt cgccgaagag ttccgcgccg
      421 ttgtcgatga tgccgttgcc gttcaaatcg cggacgagta aaccgtcatc ggcagaaacc
      481 caaccggtgg cggtgcggat gccttgattg cggtggtcga acaatgcacc tgcaaagcct
      541 ttggcagcaa cggtttctat accgtcgccg tctagatcta gggcgagggg gtcgtagaca
      601 tgatatttgc ctgaacgttt gggatctaat ttcaaccact ctcttgccca ttcagggaat
      661 tcaggcagcc aatcttcaaa tttcggaaat tggtcccatt ctttttcaag ataagggaga
      721 aaatcttgtt tataccattt ttccagtaac tctccccatt cacttatccc tcgtccaata
      781 taatcttcgc cccctttcca agggtttgca tcatcaggaa ctaaggaata tataaaccta
      841 gatacttctt ctaatacatt gctgtatgta tcaggatccg caaaaaagtt tgctatctta
      901 cttggtgtaa aatcatctat attcattata gattcgccaa gcctctcgtt tccataatcc
      961 tttattttaa atcccaaata attcaattca tgctgtaaaa cagtagagga ttttagcatt
     1021 ctagaattaa ggacatcatt taaaccagtt tttaatttag ttggtaagaa tttgctttga
     1081 gccatcgcct ctgtttgttg ttttatatat tgtaatccat caccagcagc aatgaatata
     1141 tcaccaattg cttgttgaat atcattaggt ttaaattttc ctgtttctcg tatatctcga
     1201 gatacatttc ttaaatttaa aaaaatgtta tcgagggatg caagtaaaga agcagtattt
     1261 gttattttgt tatctcttcc aatactgctt aaaaagctat ctgcagcttg gatagcatta
     1321 ttaacagtaa tttccatttt atttctattg ttatccacat tatagattaa atcagataag
     1381 gattgaatag aaccaataaa tgaatcccaa gctttatatc ccaagagtgg tgaatagggt
     1441 gttaatttca catctccaaa accgttaata ttaaaatttg cagatgtcat atctgctttc
     1501 ctttcaagta aattaaaaaa ataacgtgag gaattaaaat taagaataaa ttaattatcg
     1561 actcaatata atatgaatca attagctctc ttatggtaaa tataagaggt aaaaatatta
     1621 aaaataaact aataaagttc agaaaaatag taaatggaat attttcatat ggaaaaattc
     1681 tacaaaatac aagtaatatt ccatgacata ttagcaaatg agtaaaattt attataaaat
     1741 atataaatat ttccccatcg taagatatat tcaaataaag acatataaac aatatgaagt
     1801 atataaaata tattttttgg catctaaaaa ataaactttt agcaatcata tataaatagc
     1861 ttgtagaata aaaaacgaaa attattaatt atcgtaggtg tgcatttatt ccaacacggt
     1921 ttcatgcagc ttaaaaacca aaaattcttt ctccctttat cctttcttaa aaatagcgtt
     1981 ttcaaattct gcaacgacag gtttcattca agtaggactg ccgtgattgc atcaattgct
     2041 tcgcggcatg gcatccatat cacacaacag gacatttatt ctcgggtaag tccaccttca
     2101 tcctcttccg ccagcaggaa cagttacagg ctacctaaaa tatgctctgt gctcggatag
     2161 taaatatttc ctttctcact attgtttgtc atttaatcga cagtctactc tttctctaaa
     2221 atttcgtcaa taatagttaa atacttttat ttattttaaa taatgatata catttatggt
     2281 tatttgttta gacagcctaa aacaaacggg gaaatcgtgt tttcccgaac atggctaatg
     2341 gatagcattt tgttcgtgaa gcggctgtgc agattgtcgg ctgctaaaag taaatccccc
     2401 atttttgcgg ttgtaccgtc tgttttggta tagctgcctt gctgagccaa agtgttaccg
     2461 ttaccgagat ttttatttac atctttatag gcgagatcca aagattggat acccaattct
     2521 tcaagggtac gcaattcatt agcttgggaa atgccgtcct ggttgagatc ctgccataca
     2581 cgcagggatt ggaatgcggc gtctgccgcg ttgatgatgt tgtcgccgtt tgaatccaat
     2641 tcggccaaag ctgcatagcc gtgtttggca aaagaaccgt ctgccagttt ggtgttgtcg
     2701 ccgaagagtt ccgcgccgtt gtcgatgatg ccgttgccgt tcaaatcgcg cacaagcaga
     2761 ccgtcatcgg cagaaaccca accggtggcg gtgcggatgc cttgattgcg gtggtcgaac
     2821 aatgcacctg caaagccttt ggtggcaacg gtttctatac cgtctccgtc caaatctagg
     2881 gcgagggggt cgtagacatg atatttgcca ttgcgttttt tagtttcttt aacccaagga
     2941 gcgaaacatt ggtgaccatc atcgatccag ccttccggat ttgggaacag atctctaaat
     3001 tgcttgatgt tattattaac gatctcaaat attccagtcc actctctttt tgcaacactt
     3061 ttatataaat cattgattat cccattaccc caacctccaa ttttttgcca accatctatt
     3121 gtatattcaa cacctaactt tgttacatcc attatcacag attgtaaaaa agtaaaatgc
     3181 ttctctttaa aagatccatc aaatccttta tctaaagcag atattaactg ctttctatta
     3241 tccg
//
LOCUS       DAWEWP010000185         3206 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWEWP010000185 DAWEWP010000000
VERSION     DAWEWP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.185"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(93..1193)
                     /locus_tag="WGN53_001867"
     CDS             complement(93..1193)
                     /locus_tag="WGN53_001867"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ0140811.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADTRQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1518..3092
                     /locus_tag="WGN53_001868"
     CDS             1518..3092
                     /locus_tag="WGN53_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ0140812.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          662 a          802 c          941 g          801 t
ORIGIN      
        1 gtttaagtgt aaatggattt taagggtttg taaacaaaag gggcgaaaat gccgtctgag
       61 cggcggaaat ggctttcaga cggtatttgc gctcaataat aatatcccgc gcccagaata
      121 cacggtttgg atgcgccggt tgctttgtgc gggctgccgg gaatgcggtt aatccaacac
      181 gccgccaacc acgcaaatgc ggctgcctcc acccattgag gatcgaggtt cagttcggcg
      241 gtgctgtgca gggaaacgcg tgtgccgaaa cattctgcca aatccgccat taaaacagga
      301 ttgcggatgc cgccgccgca aatatacatt tgacgggtat ctgccgctgc gtgtgagacg
      361 gcgtcgaaaa cggtttgcgc ggtaaaacgg gaaagcgtcc gcaatacgtc gtatcggttt
      421 tcgccgccgt caaggtaggt ttcgagccaa tttagggcaa acagttcgcg ccccgtgctt
      481 ttggggtggg gtcgtgcgaa atacgggtgg gcgagcagcc tgtcgagcag ttgcggcaat
      541 atgttgcctt gtgccgcctt tgcaccgttt ttgtcgtaag gaagctgcca gtgtgcctgc
      601 gtccacgcgt ccatcagcat attgcccggc cctgtgtcga agccgaaggc gggtgcgtcg
      661 ggggggagta cgctgatgtt ggcaatcccg ccgatgttca gtaccgcgcg tgtttccctg
      721 ttgtcgcgga acagggcttc gtgaaaggcg gggacgagtg gcgcgccttg tccgccggcc
      781 gcaaggtcgc ggctgcggaa gtcgccgacg gtaaaaatcc gcgtccgttc cgccagcagc
      841 ggcaaatcgg caagctgtat gctgtaaccg tgttccggcg cgtgtcggac ggtttgcccg
      901 tggcagccga gggcggtaat gtcggatggt gcgaggtttt gactgcacag cagttcggcg
      961 gcggtttgcg cgtacaggcg gctgagttct tgcgacaaaa tcctgctgcg gtgcagttcg
     1021 tctgcgcctg tgtcctgcaa atccagcaat tggcggcgca gcctgtcggg atagggggta
     1081 aaggcgtgcc cttccgcgcc cagccatttg ccgccgtcca tccgtatcag tacggcatcc
     1141 gccccgtcca tgctggttcc cgacatgatg ccgatgtaaa gctgtgtttc catcatcact
     1201 cccaaactgg tgcaaaacgc cattttaacg tgtattgacg ctcgtatacc gatttgccgc
     1261 cgcagtgtaa ataaagtgta aataaatgtt tcaagacgga tggaaaaata ttataatgcg
     1321 cccgcaacat ccagtagtag aagtgtcata caaaccgttt ccggcagcag ttttgcattc
     1381 ggtcaggttt gggggtattt ggatgcggtt cagaaggaag gatgcgtctg ccatatcccg
     1441 aaacggcagt tcgaccggag gcagcagtac agtgtcggca acactcatga tttccaccac
     1501 attaaaggaa gattgccatg gctcaaatcc aaatgagcgc aaatgttaaa accatcaacg
     1561 ccgtctttgc cgccatgctg gtaggtacag tcggctattt tatttattgg ggcttgggtt
     1621 atacccatta caattacgcc gccttattca ttattgccac gatgttcggc gtgtttatgg
     1681 cgttcaacat cggcggcaac gatgttgcca attctttcgg caccagcgtc ggtgcgggta
     1741 cgctgaccat cccgcaggct ttgctgattg cggcggtatt tgaggtcagc ggcgcggtca
     1801 tcgcgggcgg cgaggtaacc aataccatac gcaaaggcat cgtcgatttg aagggtgttg
     1861 atttcgaacc catacagttt gtgtttatta tgatgtccgc gcttttggcg gcggcgttgt
     1921 ggctgttgtt tgcctcgaaa aaagggcttc cggtatctac cacccattcc attatcggcg
     1981 gcattgtcgg cagcgcggta tgtatggcgg taatgaacga tgccgcatcg ggcgatttga
     2041 tacgttgggg caagctgggc ggtattggtg tttcttgggt attgtcgccc gtgttgggcg
     2101 gcgcggtgtc ctatttcctg ttttcgcgcg tcaagaaaaa cgtcttagat tacaacgctt
     2161 gggcggaagg cacgctcaag ggcatcaagc aggaaaaaaa ggcctataaa gaacggcacc
     2221 gcctgttttt cgagggtttg tccgaagccg aaaaagtcga gtacgccacc aaaatggcgc
     2281 acgacgcgca aatttacgac gaacccgaat tcgatccgca agagctgcaa tcggagtatt
     2341 accgcggtct ttatgcgttc gacaaccgta aaaacaatgt cgattcctac aaagcactgc
     2401 attcttggat tccctttatc gcttcgttcg gcgcgatgat gatttccgct atgctgattt
     2461 tcaagggctt gaaaaacctg catttgggga tgagcaacgt caacagcttc ctgaccatct
     2521 ttatgatagg cgcggcggtg tggatgggga cgtttgtgtt tgccaaaagc ctcaagcgta
     2581 aagacttggg caaatcgacc tttcagatgt tttcatggat gcaggtcttt accgcctgcg
     2641 gcttcgcatt cagccacggg gcgaacgata tcgccaacgc catcggtccg tttgccgcga
     2701 ttatggatgt tttgcgtacc aacagcgttg ccgcgcaaaa tgtcgtcccc ccgattgcga
     2761 tgctgacttt cggcatcgcg ctgattgtcg gtttgtggtt tgtcggtaaa gaggtgatta
     2821 aaaccgtcgg tacgagtttg gcggaaatgc accccgcctc gggttttacc gccgaactgt
     2881 ccgccgcatc cgtcgtgatg ggcgcgtcgc tgatggggct gcccgtgtcc agtacgcata
     2941 tcttggtcgg cgcggtactc ggcatcggtc tggtcaaccg caatgccaac tggaaactga
     3001 tgaagcccat cggtttggcg tgggtcatta ccctgcctgc cgccgccgta ttgtcggttg
     3061 tctgctactt ggttttacag gcagtattct gattgtaaaa tactgatgcc gtctgaaccc
     3121 gtgttcagac ggcattttgt tgatggaatg tgcgggcttg tgccttatgc acaatctgtt
     3181 ctgtcgggat atgccgtttg gtatag
//
LOCUS       DAWEWP010000186         3158 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWEWP010000186 DAWEWP010000000
VERSION     DAWEWP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.186"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(127..999)
                     /gene="hpnD"
                     /locus_tag="WGN53_001869"
     CDS             complement(127..999)
                     /gene="hpnD"
                     /locus_tag="WGN53_001869"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ0140813.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLPQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGEMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLQYADKMGLALQLT
                     NIIRDVGEDARNGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            1170..3032
                     /gene="hscA"
                     /locus_tag="WGN53_001870"
     CDS             1170..3032
                     /gene="hscA"
                     /locus_tag="WGN53_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ0140814.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
BASE COUNT          730 a          949 c          837 g          642 t
ORIGIN      
        1 atttcaaagc gcgaacgcca acgggattgc gcggttacga tgcagacttt cagacgatgg
       61 ttgaaacccc gtccggcggg gctgtgggaa tcgggtttgc ctgacgggcg gcgggcggta
      121 tgcgccttat gcccgttccg gcgtgccggg gcgcggtttg aatccgaata accaggtttt
      181 cagggcaatg cgttttttgc gcggcgaagg gagggcgatt ttgtatttga ggacgttttg
      241 cgcgccgtct cggtcgattt cgtttaaaag cgcgtaataa accgccgcca tgaccagtcc
      301 gactttttgg gcttttttat cggcatcagg cagcagcgat acggcttcgc ggtaggtttc
      361 gcgggcgcgt ttgatttgga acgccatcaa ttcggcaaaa ttgcccgtcg ggctgcattg
      421 caaaatcacg cttgcgggta cgtcaaaccg ctgcatttcc tccattggca ggtaaatcct
      481 gccgttgcgc gcatcttcgc cgacatcgcg gatgatgttg gtcagttgca gcgcaagtcc
      541 cattttgtcg gcgtattgca gcgttttgcc gtctgaaaac cccaaaatcc gcgcaatcag
      601 gcagccgacc acgcctgcaa cacggcggca gtacagtttc aattcttcaa aactgccgta
      661 acgcgcccga accaaatcca tctgcatccc gtcgattaag gcttccaatt catatttcgg
      721 cagcttgaag gtttccttaa cctgccgcaa agcctgattg acggggtgtt ccggcatctc
      781 gccaccgaac accttgtcca aatcgccgcg ccaccagttc aatgttgcct gtgcaacatc
      841 ggggttggaa cattcgtcaa ccacatcgtc caattcgcgg caaaaggcat acagaaccgt
      901 taccgcatcc cgtttttcct gcggcaggaa acggaagccc gacaaaaaac tggagcggct
      961 ttcttctgct ttttggcggc aatagtcgag tcctttcacg atttatattc ctaatgatgg
     1021 gcgggaaagg cggattttat cggcatttgg cggcagaggg caatttcggc ggcacggccc
     1081 aatccttagc ggtttgctca actatcggcg caaattctgt taaaatgccg ccttccttcc
     1141 tttacacacc gcaccgacag gcagaattta tggctctttt gcagatttca gaaccgggta
     1201 tgtccgccgc cccgcaccgg caccgtttgg cggcaggcat cgatttgggt acgaccaaca
     1261 gcttggttgc caccgtccgc agcggcagtg cggcctgcct gcccgatgcc gacgggcgcg
     1321 ttaccctgcc ttccgtcgtc cgctatctgg aaaacggcgg cattgaagtc ggcaaaaccg
     1381 ccctgtccgc ccaaaaaacc gacccgctga acaccgtcag ctccgccaaa cgccttatcg
     1441 ggcggacgct tgccgatctg catcaaaata cgcattacct gccttaccgt ttcggcgaca
     1501 atcaacgcgt tatcgaactg catacgcggc agggggtgaa aacgcctgtc gaagtgtcgg
     1561 cggaaatcct caaaaccctt aaatcgcgcg ccgaagaaac cttgggcggc gatttggtcg
     1621 gcgtggtgat taccgttccc gcctatttcg acgacgcgca acgccaagcc accaaagatg
     1681 ccgcgcgtct ggcgggtttg aacgtcttac gcctgcttaa cgaacccacc gccgccgcaa
     1741 tcgcctacgg gctggacaac gcctcggaag gcacttttgt tgtgtacgac ttagggggcg
     1801 gcacattcga cgtatccgta ttgcaactga ccaaaggact gtttgaagtc aaagccaccg
     1861 gcggcaacag cgcgttgggc ggcgacgatt tcgaccaccg cctgttctgc cgcctgctcg
     1921 aacaaaacgg actctcccaa ctcaacgaac aagacagcca actcctgctc tcgctcgtcc
     1981 gcgccgccaa agaacaatta accacgcaaa ccgaagcgcg cattcaggcg acgctttcag
     2041 acggcatggc aatcgacaca agcatcagtc gcgccgagtt ccacaacctg acgcagcatt
     2101 tggtgatgaa aacgctcgaa ccggtcacac aggcgttgaa agatgccggt gtcggtaaaa
     2161 acgaagtcaa aggcgtgatt atggtcggcg gttcgacccg tatgctgcac gtccaacagg
     2221 cagtcgccac cttcttcgga caaaccccgc tgaacaacct caatcccgac gaagtcgtcg
     2281 cgctcggcgc cgccatacag gcaaacgtcc tcgcaggcaa caaaaccgac ggcgaatggc
     2341 tgctgctgga cgttacacct ttgtcgctcg gtttggaaac ctacggcggc ttggcggaaa
     2401 aaatcatccc gcgcaattcc accatcccca ccgcgcgcgc gcaggacttt accaccttca
     2461 aagacggtca aaccgcgatg acgatacacg tcgtgcaagg cgagcgcgag ctcgtttctg
     2521 actgccgcag ccttgccaaa ttcaccctgc gcggcattcc accgatggcg gcgggtgcgg
     2581 cgcgtatccg cgtaaccttc caaatcgatg cggacgggct gctgtccgtt tccgcccaag
     2641 aacaaagcac cggcgtacag gcgcaaatcg aagtcaaacc ttcctacggc ttggacgacg
     2701 gcgcgattac ccgaatgctt aaagacagta tggacaacgc cgccgaagat atggcggcac
     2761 gcgcccgcgc cgaagccgta gtcgaagccg aaagcctgac cgatgccgtc aacgccgccc
     2821 tcgagttgga cagcgattta ctggatgcca aagaattgca acagatccgg caaggcatcg
     2881 ccgatttgca aggccgtctg aaagacggaa aagccgaaga catccgctcc gccgtcgcca
     2941 aactcagccg cagcaccgac aacttcgccg ccaaacgcat gaaccgcaat atccaacgcg
     3001 cattgacagg ccagagtgtc gataatattt gatacttaaa cggtttcaga cggcatagat
     3061 ataacctgat gccgtctgaa ggctcgaaaa cacttgaaaa acatcgatat ggaaaagtca
     3121 ggcattgtct attcgatgaa aaccgtcatc aagggcgt
//
LOCUS       DAWEWP010000187         3101 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWEWP010000187 DAWEWP010000000
VERSION     DAWEWP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.187"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..211)
                     /locus_tag="WGN53_001871"
     CDS             complement(<1..211)
                     /locus_tag="WGN53_001871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140815.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQ"
     gene            271..894
                     /locus_tag="WGN53_001872"
     CDS             271..894
                     /locus_tag="WGN53_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0140816.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1005..2387
                     /locus_tag="WGN53_001873"
     CDS             1005..2387
                     /locus_tag="WGN53_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0140817.1"
                     /translation="MARDNRIQMFPHEWRTSTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLIAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2391..2915
                     /locus_tag="WGN53_001874"
     CDS             2391..2915
                     /locus_tag="WGN53_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ0140818.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          623 a          839 c          937 g          702 t
ORIGIN      
        1 cctgctgccg cccgaaaagc gtgatgcgct gatggtgctg ctgcataatc cggacgacgg
       61 ataaccggtg aaaacggaaa tgcccgggcg gtgttgcctg tgcctgtttg gacaagcgtt
      121 cttgagggcg gtagaattga ggtttgcccg aaaagggcag ggcgatatgc cgcgcttgga
      181 ttttgcgcgc ggtttttcgc gttggaagca atcattgtct gtttgaatat ttaatgccgt
      241 ctgaaacgtc cggacgcgcc cccgttccct atgaaaaaac cgaccgatac cctacccgtt
      301 aatctgcaac gccgccgcct gttgtgtgcc gccggtgcgt tgttgctcag tcctctggcg
      361 cacgccggcg cgcaacgtga ggaaacgctt gccgacgatg tggcttccgt gatgaggagt
      421 tctgtcggca gcgtcaatcc gccgaggctg gtgtttgaca atccgaaaga gggcgaacgt
      481 tggttgtctg ccatgtcggc acgtttggca aggttcgtcc ccgaggagga ggagcggcgc
      541 aggctgctgg tcaatatcca gtacgaaagc agccgggccg gtttggatac gcagattgtg
      601 ttggggctga ttgaggtgga aagcgcgttc cgccagtatg caatcagcgg tgtcggcgcg
      661 cgcggcctga tgcaggttat gccgttttgg aaaaactaca tcggcaaacc ggcgcacaac
      721 ctgttcgaca tccgcaccaa cctgcgttac ggctgtacca tcctgcgcca ttaccggaat
      781 cttgaaaaag gcaacatcgt ccgcgcgctt gcccgcttta acggcagctt gggcagcaat
      841 aaatatccga acgccgtttt gggcgcgtgg cgcaaccgct ggcagtggcg ttgattttga
      901 acccgcgccg caaccgaaat acggcgaatc ctgtataatc cgaaaatctg ttcactggaa
      961 gttcagacgg cattgcaact gttgatgccg tctgaaaaaa tatgatggca agagacaacc
     1021 gcatccaaat gtttccgcac gaatggcgca ccagtacgac gctttccggc gtgtacgcgc
     1081 tgcgtatgct gggtatgttc ctcgtgctgc ccgtattggc ggtgtatgcc gcctcgctgc
     1141 ccggcgcgga aggcaacaaa acgctggtcg ggctggcaat gggcatttac gggctgacac
     1201 aggctctgct gcaactgcct ttgggcatcg cttccgacaa gttcgggcgc aagaaaacca
     1261 tttatgtcgg gctgattgtg tttgcagcgg gcagcttcct tgccgccgcc gccgatacgc
     1321 tgcccatgct gatcgccgca cgcgccatac agggtgcggg agcggttagt gcggcggtta
     1381 ccgcgctgct ggcggatttg acgcgcgacg gcgtacgtac ccgcgcaatg gcgatgatcg
     1441 gtttgagtat cggtctgacg ttttcggtca gcctcgtcgt tgcccctgtg attgccgacg
     1501 cggtgggcgt acgcggcttg tttctgctga ccggtatttt gaccgttgcc agtatcggcg
     1561 tggtggcgtg gatgactccc gacccggagg tttccaagct gcacgaagat acgcaggcgc
     1621 agccttcgcg cataggcgaa gttttgaaaa accgtaggct gctgacgctc gatttcggca
     1681 ttttcgccct gcacgccgcg caaatggcat tgtttaccgc gctgcctttc gcgatgaccc
     1741 agctcggttt ggaaaaaata cagcattgga aagtctatct gccctcgacc attacgggct
     1801 tggtggtgat ggttccgctg attatcgtcg gcgagacgcg caacaagctc aaacaggttt
     1861 ttgttttggg tatcgtctgt attgcggcgg cgcagctcgg tttgctgtcc ggtatgcgct
     1921 cggtaggctt gattaccgct tatttggttg tttactttat cggttttaat gtgttggaag
     1981 cgagcctgcc gtctatggtt tccaaaatcg cgccgtccga cctgaagggt acggcgatgg
     2041 gcgtgtacaa cacgatgcag tcgctcggac tgtttgccgg cggcgcggca ggcggtttgc
     2101 tgtttcaaaa atacggcttt tccggcgtgt ttgccttttg cagtatattg atgctgctgt
     2161 ggctggtaat tgccgtttta tcgcctgcgc ccaagcccgt caaaaacctc agttaccctg
     2221 tcggcggcgt gtggcagggc aatcaggaag ggttataccg cgccttgtcg gagcttgagg
     2281 gtgtggaaga catcggtttc agtttcgacg ggcagaccgt ctatctcaaa gtgttgcaga
     2341 agggtttcga tcaggctgcc gctgaaaaaa tcatcacagg agtttaaaaa atgtcattga
     2401 acaaagtcat cctcatcggc cgcctcggac gcgatcccga agtgcgctat atgcccaacg
     2461 gcgaggcggt ttgtaatttc agcgtcgcca ccagcgaaac ttggaacgac cgcaacggcc
     2521 aacgtgtaga gcgtactgag tggcacaaca tcaccatgta ccgcaaactg gcggaaattg
     2581 ccgggcaata cctcaaaaaa ggcgggctgg tttatctgga aggcagaatc caaagccgca
     2641 aataccaagg caaagacggc atcgaacgca ccgcttacga tattgtcgcc aacgaaatga
     2701 aaatgttggg cgggcgcaat gaaaacagcg gcggtgcgcc ttacgaggaa ggttacggtc
     2761 agagtcagga ggcttaccaa cgccccgcgc agcaaagccg gcagcccgcc tccgacgcgc
     2821 cgtcccatcc ccaagaagca ccagccgcgc cgcgccgcca acccgtgcct gccgccgccc
     2881 cggtcgagga cattgacgac gatatcccgt tctgaatttt acggccggac atcctcgcgg
     2941 agggaaatca taaaggacgg agaaacctta aaccttacgg ggcagggttt ctcttttttg
     3001 catttgggcc ggcttgttgc cgccctttgc gttgggcgga acgttgcgat tgcgccgttc
     3061 gggcgttttg aggcagtgat gcctgccgca cgtccccgct g
//
LOCUS       DAWEWP010000188         3097 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWEWP010000188 DAWEWP010000000
VERSION     DAWEWP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.188"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(311..787)
                     /gene="greA"
                     /locus_tag="WGN53_001875"
     CDS             complement(311..787)
                     /gene="greA"
                     /locus_tag="WGN53_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ0140819.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1182..2483
                     /gene="aroA"
                     /locus_tag="WGN53_001876"
     CDS             1182..2483
                     /gene="aroA"
                     /locus_tag="WGN53_001876"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ0140820.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGKRQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     ENYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPKTLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETTE"
     gene            complement(2526..3059)
                     /locus_tag="WGN53_001877"
     CDS             complement(2526..3059)
                     /locus_tag="WGN53_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0140821.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          648 a          829 c          841 g          779 t
ORIGIN      
        1 attttaggtt tctgattttg gttttctgtt tttgagggaa tgacgggatg caggttttct
       61 taaccctgcg tcctagattc ccgcttttgc gggaatgacg gcgacagggt tgctgttata
      121 gtggatgaac aaaaaccagt acggggttgt ctcgccttag ctcaaagaga acgattctct
      181 aaggtgctga agcaccaagt gaatcggttt cgtactatct gtactgtctg cggcttcgtc
      241 gccttgtcct gatttttgtt aatccactat aatttcctgc gtgtgtcggg tgtatcgaaa
      301 tcaagccgaa tcaaatatat cggacttcga taatgtcgta ttcgcgcacg ccgcctgggg
      361 cttggacttc cgccgtatcg ccctcttcct tgccgattaa ggcgcgggca atcggtgagc
      421 cgacatagat tttgccctgt ttgatgtcgg cttcgtcttc gccgacaatt tgataggtaa
      481 cgtgttcttc cgtttccaaa tcttccagcg taaccgtcgt accgaacacg attttgcctt
      541 cggcgtggat ttcggtcgga ttgatgatgt gggcaacgga aagtttgtgt tccagctcgg
      601 aaatgcggcc ctcgataaag ccttggcgtt ctttggcggc ttcgtattcg gcgttttcgg
      661 acaaatcgcc gtgcgaacgg gcttcggcaa tcgcttcgat cacttcggga cgcgccacgc
      721 ttttgagctg ctgcaattcc tgtttcagca attccgcacc gcgtacggtc agggggattt
      781 tttgcatcgg tcttttttct ccatattccg gcacaccgtt ttgcggcagc aagcataccg
      841 cgtaccgtct tgttttgtgc gtccggatat taaaataaaa atacaagccg cccggaaaat
      901 cggcggcttg tctgtcgttg aacagcggct attctaccaa attctatgaa attggcaatc
      961 gtgccgtgcc gccgacaaac gcgccatgtc cgcaacaaaa gctgaaaata tgccgtcaaa
     1021 gaaattttag aaacaaaaaa tttaaaaata atcaattttc ggcataaaaa accacattta
     1081 cggactttaa aaccgaaaat gccaagcctg agatttttca tacagcattt gcaccagtat
     1141 aatgcaggct gtttttatct ttataatatt gacgttttgc catgaccgaa tccgtccgcc
     1201 tccccgtcgc ccgtctcaaa ccttccaccg tcgccctgcc cggctccaaa agcatcagca
     1261 accgcaccct gctgcttgcc gccttgtccg acaatgcttg cgaaatccat tccctgctca
     1321 aatccgacga taccgaccgt atgctcgaag cactcgataa actcggcgtt caaatcgaat
     1381 atcttgccga agaccgtctg aaagtgcacg gcacaggcgg acgcttcccc aaccgcactg
     1441 ccgatttgtt tttgggcaac gcgggcacgg cgttccgtcc gctgactgcc gcacttgccg
     1501 ttttgggcgg cgattatcat ctgcacggcg tgcctcgtat gcacgaacgc cccatcggcg
     1561 atttggccga tgcgctgcgg attgccggcg ccgatgtcga atatctcggc aaggaacact
     1621 atccgccgct tcatatcggc aaacgtcaag acaacggcga acgcgtgatt ccgattaaag
     1681 gcaatgtgtc cagccagttt ctgaccgccc ttttaatggc gttgccgctg accgggcagg
     1741 cgtttgaaat ccgtatggtc ggcgaattga tttccaagcc ctatatcgac attactttaa
     1801 aactgatggc gcaattcggc gtacaggttg ccaatgaaaa ctaccgcgtc ttcaaaattc
     1861 ccgccgatgc gcactaccac gcgcccgaac acttgcacgt cgaaggcgat gcctccagcg
     1921 cgtcctactt cctcgcagcc ggtttgattg ctgccacgcc cgtgcgcgtg accggcatcg
     1981 gcgcaaacag catacagggc gatgtcgcct ttgcccgcga gctggaaaaa atcggggcgg
     2041 acgtggtttg gggcgaaaac ttcgtcgaag tttcacgccc gaaggaacgt gccgtccaat
     2101 cctttgattt ggatgcgaac catatccccg atgccgccat gaccctcgcc atcgtcgcgc
     2161 ttgctacagg gcaaacctgc acgctgcgca acatcggttc gtggcgcgtc aaagaaaccg
     2221 accgcatcgc cgcgatggca aacgagttgc gcaaactcgg ggcaaaagtc gtcgaagaag
     2281 ccgaagcaat tcacatcacc ccgcccaaaa cgctgacacc cgacgccgtc atcgacacgt
     2341 acgacgacca ccgcatggcg atgtgtttct cgctggtttc gctgttgggc gtacccgtcg
     2401 tcatcaacga tccgaaatgc acccataaaa ccttcccgac ttatttcgac gtgttctctt
     2461 cactgaccga aacaacggaa taaggcggca ttttgccgcg attccggcgc ggcgcggcgg
     2521 gcggctcatt ctgtaaagaa agtatgtgcg ccgaggtagt ttttggcgta aaacggtgtg
     2581 gagagttttt cggttttgat ggttttgccg ctgctggggg catggatgaa ttcgccgttg
     2641 ccgatataga gtccgacgtg tgagtagcgg tgtgtgccgc cggtgttgaa gaatacgagg
     2701 tcgccggcct taaggcggct gtcggggatt ttgcggcttg ccgccgccat gtcgcgggcg
     2761 gtgcgcggca gcttgacgtt gagggcgttt ttgtaaacga attgaatcat gccgctgcaa
     2821 tcgaagccgg ttgcggtgct gctgccgccc catttgtagg gcgtgccgat gagtccgagg
     2881 ctgtggagca tgagttcctg cgagccttgt gtgcggtcga tgtggctgat gcggacggct
     2941 tggatttgcc ggactgtctg tttgggtttc ggttggcggt gtttgccgga ggtcgtgccg
     3001 catgaggcga ggagcagtgc gctgagacag aggaaaaggg ttttgtcggg gggaaacatg
     3061 gtttttcctt tgcgggttcg gatatccgtc tgaaggt
//
LOCUS       DAWEWP010000189         3076 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWEWP010000189 DAWEWP010000000
VERSION     DAWEWP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.189"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..258)
                     /locus_tag="WGN53_001878"
     CDS             complement(<1..258)
                     /locus_tag="WGN53_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140822.1"
                     /translation="MNMENYFSNLNLDIRTHKLGTFTDQKVTPDVLCAVAECIIEYIE
                     NIGEIFSINDIRYSDYAEYIATAVFKKPSIENAGSEYNKFFS"
     gene            complement(270..2858)
                     /gene="dcm"
                     /locus_tag="WGN53_001879"
     CDS             complement(270..2858)
                     /gene="dcm"
                     /locus_tag="WGN53_001879"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0140823.1"
                     /translation="MKNQYSKSLHGVFYLAFYDKIHAFFAEQLFQLIINMNKKPTYIS
                     LFSSAGVGCYGFKTNGFECVATNELLEKRLKIQKYNQKCRFDSGYIAGDITLPETQAK
                     LFAEIRRWNTEIDVVIATPPCQGMSVANHKKNNEMARNSLVVESIKIVREVQPKFFIF
                     ENVKAFLKTPCTDTDYQEKPIEEAIWSNLGGHYNILSNVLNFKDYGANSSRTRTLVIG
                     VRKDIHHITPYDIFPKQTPPKTLRTLLADLPPLMQMGEISDDDIYHSYREFDRRMLPW
                     IAQLKEGESAFQNTDSARIPHQIKNGEIIYNQNKNGDKYARWFLDREGPCIHTRNDIL
                     ASQNTIHPTENRVFSVRELMRMMSIPPSFKWTAQDEKQLNALSFDEKKTFLKKEELNI
                     RHCIGESVPTAVLANVAQNIQAALQQSELSMTEIQNLIKRENLSLADNLYRFIEQHFD
                     AYPFSRLLQIAEYANAARSETAAYFTRIDIAFGLVNALPDFKKKKNLRILEPSVGIGN
                     FIPLLAKKYADKEKVIFDLVDIDSNSLNLLSLLLKKLNLGSKFEFNIIHQDFLTATLP
                     ENYDLVIGNPPYGKVKASDKNLALYRKNATNKDTQNIFSFFIDKALTLSKTVALVVPK
                     SVINAPEFQITRQQLITANINRIIDYGEKAFKGVKIETIAFIADQTDKDNQHITIESH
                     IQETLIQNCKNYFFSDNFPYWLIYRNSEFDQIAQKLEFNVFQSFRDRQITKAHTQNSG
                     NVRVLKSRNIGNNEIIDIEGYDSYVDSVAPFSVGKFINRKNVVMIPNLTYKPRAAFLP
                     ENSIADGSVALLTMKKHEKILTQRDLAYYATPEFEQFYRVARNYGSRSMNIDNHSVFF
                     FGLLKD"
BASE COUNT          899 a          566 c          606 g         1005 t
ORIGIN      
        1 tgaaaaaaat ttattgtatt cgctaccggc attttcaatg gatggttttt taaatacggc
       61 ggtggcgata tattcggcat aatctgaata acgaatatcg ttaatgctaa atatttctcc
      121 aatattttca atatattcga tgatacattc tgctacggca cataatacat ccggggttac
      181 tttttgatcg gtaaatgtgc cgagtttatg tgttctgatg tctaaattta agttagaaaa
      241 ataattttcc atattcattt tttactcctc taatctttta gcaatccgaa aaaaaacaca
      301 gaatgattgt ctatattcat tgaacgagaa ccataattac gggcaacacg gtaaaactgc
      361 tcaaattcgg gagttgcata gtatgcgaga tctcgttggg ttaggatttt ttcgtgtttt
      421 ttcattgtca gtagggcaac cgagccatca gcaatactgt tttcaggcaa aaatgctgcc
      481 cgaggtttgt aagtcagatt gggaatcatc acgacatttt tacggttaat aaatttgccg
      541 acagaaaaag gggcaactga atccacataa ctatcataac cctcaatatc aataatctcg
      601 ttgttaccga tattacggga ttttaaaacc cgaacattgc cactattctg tgtatgcgct
      661 ttggtaattt ggcgatctcg gaatgattga aatacattaa attccaattt ttgtgcaatt
      721 tgatcaaatt cactattacg ataaatcaac caatagggaa aattatcaga aaaaaaatag
      781 tttttacagt tttgtatcaa cgtttcttgg atatggcttt ctatcgtaat atgttgattg
      841 tctttgtcgg tttgatcggc aataaaggca atcgtctcta tttttacgcc tttaaatgct
      901 ttctcgccgt aatcaataat gcggttgata ttggctgtga taagttgttg tcgggttatt
      961 tgaaactcgg gggcgttaat cacacttttg ggaacaacca aagccacggt tttactgagc
     1021 gtcagagcct tatcaataaa aaatgaaaaa atattttgcg tatctttatt ggtagcattt
     1081 ttacggtata gtgccaaatt tttatcactt gccttaactt tgccgtatgg tggattgccg
     1141 atgactagat catagttttc aggcagcgtt gcggttaaaa aatcctgatg aataatattg
     1201 aactcaaatt tactgcctaa atttaatttt ttcagcagca atgacaggag atttaaactg
     1261 tttgagtcaa tatccaccaa atcaaaaata actttttctt tgtctgcata tttttttgcc
     1321 agtagtggaa tgaaattacc aatgccgaca gagggttcta aaatccgtaa gttttttttc
     1381 tttttgaaat caggaagagc attaaccagt ccgaaagcaa tatcaatacg ggtaaaataa
     1441 gcagccgttt cgctacgggc agcattagcg tattcagcaa tttgcaaaag ccgactgaat
     1501 ggatatgcgt caaaatgttg ctcaataaat cggtagagat tatcggcaag ggataaattt
     1561 tctcgtttga ttaaattttg aatttccgtc attgacagtt cggactgttg caaggctgcc
     1621 tgaatgtttt gtgcgacatt ggccaaaaca gcggttggca cagattcacc gatacaatgg
     1681 cggatattca gctcttcttt tttcaaaaag gtttttttct catcaaatga cagtgcatta
     1741 agctgttttt catcttgtgc cgtccattta aatgatggcg gaattgacat catacgcatg
     1801 agttcacgca cggaaaatac acggttttca gtgggatgga tggtgttttg gctggctaaa
     1861 atgtcattac gggtatggat gcacggaccc tcacgatcta aaaaccaacg ggcgtatttg
     1921 tctccatttt tgttttgatt gtagataatt tcaccatttt taatttgatg gggaatgcgt
     1981 gcagaatcag tattttggaa agctgactcg ccttctttaa gctgtgcaat ccacggcaac
     2041 atacggcgat caaattcacg atagctatgg taaatatcat catcgctaat ttcgcccatt
     2101 tgcattaaag gcggcagatc agccagcaat gtacgcaagg tttttggcgg tgtttgcttg
     2161 gggaaaatat cgtacggcgt aatgtgatga atatcttttc gcacgccaat caccagcgtt
     2221 ctggtacgac tggaatttgc accataatct ttaaaattta gtacattgga tagaatgttg
     2281 taatgtccgc ccaaatttga ccaaatggct tcctcaatcg gcttttcttg ataatcggta
     2341 tcggtgcagg gtgtttttaa aaaggctttg acgttttcaa aaataaaaaa cttgggctga
     2401 acttcccgaa cgattttgat tgattccacg accaaagaat ttcttgccat ttcattattt
     2461 tttttatggt tggcaaccga cattccttgg caaggcggtg tggcaatcac aacatcaatt
     2521 tcagtattcc agcgtcgaat ttcggcaaac aatttggctt gggtttcagg cagggtaata
     2581 tcgccggcaa tataacctga atcaaagcgg catttttgat tatatttttg gatttttagg
     2641 cgtttttcta aaagttcgtt ggttgctacg cattcaaaac cattggtttt aaaaccatag
     2701 caaccaacgc ctgcactgct aaataaactg atgtaggtgg gttttttgtt catatttatg
     2761 atgagttgaa acaactgctc tgcaaaaaat gcgtgtattt tatcatagaa ggcaaggtaa
     2821 aacacgccat gcaatgattt actatattga tttttcatca aaaaagtgct gtttgaacag
     2881 atatacagcg gtgttttttg caatgcgttt caaacttgag gtacgttttt aatagaaaca
     2941 ccgtctgaac gccaacctga gacctttgca aaattcccac aaatccccca attcccacca
     3001 agacatttag gggatttctc atgagcactt tcttccggca aaccgcccaa gccatgattg
     3061 ccaaacacat cgaccg
//
LOCUS       DAWEWP010000190         3022 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWEWP010000190 DAWEWP010000000
VERSION     DAWEWP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.190"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            68..847
                     /locus_tag="WGN53_001880"
     CDS             68..847
                     /locus_tag="WGN53_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0140824.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALTEHPLVGEYGHSFRFLPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPDMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(1057..2469)
                     /locus_tag="WGN53_001881"
     CDS             complement(1057..2469)
                     /locus_tag="WGN53_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0140825.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVVGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRVGIGFSSPVLCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPAAAGILSDGYTEDEPCPVYVSDRCDKACV
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            2560..2636
                     /locus_tag="WGN53_001882"
     tRNA            2560..2636
                     /locus_tag="WGN53_001882"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2594..2596,aa:Arg,seq:acg)
     gene            2654..2728
                     /locus_tag="WGN53_001883"
     tRNA            2654..2728
                     /locus_tag="WGN53_001883"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2687..2689,aa:Glu,seq:ttc)
BASE COUNT          827 a          911 c          570 g          714 t
ORIGIN      
        1 accgtcaatg cggaaaaaaa cacccaaatt ggctataatc ccgacaaaca cactcaagga
       61 caacaacatg gcagcctcgc ccgaagcaaa attcactgaa gaaaagattt tgtgggtcaa
      121 acaccacacg ccgaaactca tcactttcgc catcagccgt cccgaatcct accgttttaa
      181 agcgggacag ttctcccgac tcggtttcta cgaaggggaa ggctttattt ggcgtgccta
      241 ctccatcgtt tccgcagaat atgccgacac gctcgaatat tttgccgtac tcatccaaga
      301 cggccccatg tctgcccgtt tcgccaaaat gcaacagggc gacaccatcc tgctcgataa
      361 aaatgccacc ggcttcctgc ttcccgaacg cttccccgac ggcaaggatt tggtgatgct
      421 ctgcaccggc tccggcatcg cccccttcct ttccattctc gaacaacctg aaatccggca
      481 gcgtttcgat accgtcaacc tgatacattc cgtatctttt cccgaagaat tgattttcaa
      541 cgaccggctc gccgcattga ccgaacatcc cctggtaggc gaatacggac actcgttccg
      601 tttcctccct gttaccaccc gtgccgccaa cccctcgggc ttaagcggaa aacgcattcc
      661 ggaactctta aaaaacaaca gcatcgaaca ggcgctgcat accaagttca ccccggaatc
      721 cacacggttt atgatttgcg gtaacccgga catggtcaaa gacactttcc aaacgctgct
      781 tgacatgggt tacgccatgc accgcaaccg cattcccggt caaatcatga tggaaaacgg
      841 cttctaaaaa ccaccctgct tgtccgatgc cttcggatgg acggtcaaac cgacacggca
      901 cgaaaaccgc gtcggcaaaa atgccgtctg aaaagttcag acggcatctt cggacacatt
      961 acctgcaaac ggtaacacat tccaacccaa accgattagg caatcagtac gaacgactgt
     1021 ttacatactt gccggctttc accaaccggt atcgatttaa ccgatttcct taatattttt
     1081 cctgtccgtt ttaaacttcg ccttaaacgc atccggtaaa tctttatcga aataccaaag
     1141 cccgtcatcc atttctaatg cgccgcccat tccatgcaga acaacttttt cccccgttaa
     1201 aggatcggtt tcggtaattt ccacctcatg catttttccc caatccaaga caggcagctt
     1261 gtgtgcaaac gccaaaacct gttcgtcaga gcggacgcac gccttatcac atcggtctga
     1321 aacatatacg ggacaaggct catcctctgt ataaccgtcc gacaggatac cggctgcggc
     1381 aggacacaat ccgtatgttt ccgcccacga cgacaaagcc cgtctcgctg cctttttatt
     1441 ggcaaataat ccggtaggcg gattatccgt cacaccgttt ttcaaagccg ctgttttcgc
     1501 attcaacatg ccgtctgaac ctttttcaaa cctgacggtc gtaaatgttt taagcagatt
     1561 tttggcagat acataacaac ccgaatgata acgtccgacc aattccgctt taatcttata
     1621 tgcatgcagg ctgcccaatg caggaaaaaa acggacttcc tccgtattgc accaatcaaa
     1681 cggggctttt ccggaatcca ataaagccga aacctcgcta tatacccgtt caaacgtacc
     1741 gatgtaattt acttttcctc cgccgtcgaa acaggccagc acccccatac cgtcaggcaa
     1801 accgtacaac tgttccctca accgttcggg cagcgcggca ggcaacggtt tcggattcat
     1861 caaacggaaa cactgcctga tccatgcctc gaccccgtgt tccgacagac tgtattccaa
     1921 ataatcgcac aatgccgata catccgccat cgcacgatgc ctgtcttcca caacaatccc
     1981 caacctttcg atgatactgt ccaggctgtg cttgtaaaat tgcggataca gacaccggga
     2041 cagctgcaca ctgcacaaaa caggcgatga aaatccgata cccacacgat gaaactcatg
     2101 ctttaaaaac gtatagtcga aacggctgtt atgtgcaacc agcacacaac ccttcaatac
     2161 cgaaaacaac tcgccggcaa tctctgcaaa aacaggcgca tcggcaacca tgccgtctga
     2221 aatccccgtc agccccacca caaactgcgg aatcggtttt tgaggattaa ccaaccactc
     2281 atgcctcacc acccttccct gctcaaactt gaccaaagcc acttcggtta ccctgtcttc
     2341 atacagattg ccgcccgtcg attccaaatc aaccacggca acaggcattc caaaccgtaa
     2401 aaataccttt tccagcaagg gccagcgaga agcaacaatc attttattct ctttaaattc
     2461 aaacaacaaa ccaatatttt acacttttaa ggcatttcat ccaacaaaac aattgacaga
     2521 atccgatgat taccctaaaa ttcgaatctt tcttgcagcg cacccgtagc tcagttggat
     2581 agagtatctg gctacgaacc agagggtcgg gcgttcgaat cgctccgggt gcgccagtaa
     2641 gaaaatacaa tatgcgccca tcgtctagcg gttaggacat cgccctttca cggcggtaac
     2701 cggggttcga ttccccgtgg gcgtgccaaa ttctaaatcc ccgagattat cgctcgggga
     2761 ttttttattg tctcagcaac tcgttaccat atctttacct acccccttca tcagaatctc
     2821 agacgtaatc gaatcatatt caaacctttg ccgtgcaaac cgatatccca taaccggatg
     2881 cggtgtccgt ccaacatttt acccgattga aacgcctgat atattgcacc ccatcaacgt
     2941 ggcattactt ttcttaacaa tcccctttga cagcaactga ctagggcttt tttatgccat
     3001 catcaaattt atagtggatt aa
//
LOCUS       DAWEWP010000191         2967 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWEWP010000191 DAWEWP010000000
VERSION     DAWEWP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.191"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            121..1665
                     /gene="purF"
                     /locus_tag="WGN53_001884"
     CDS             121..1665
                     /gene="purF"
                     /locus_tag="WGN53_001884"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ0140826.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIVTAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANERSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            1765..2256
                     /gene="greB"
                     /locus_tag="WGN53_001885"
     CDS             1765..2256
                     /gene="greB"
                     /locus_tag="WGN53_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ0140827.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            2315..2941
                     /locus_tag="WGN53_001886"
     CDS             2315..2941
                     /locus_tag="WGN53_001886"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ0140828.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT          713 a          908 c          775 g          571 t
ORIGIN      
        1 tattctccat aaacccacct atctgtcctt cgctcaaata ttgtcaacaa ttcaggcaaa
       61 aaaccaccgt ttgctttata attccgtttt acccttaaac ctcgaatcgg agaagacgat
      121 atgtgcggcg tattaggttt ggtcagtcat gaacccgtga accagcttct gtacgacggt
      181 ttgcagatgt tgcagcacag gggacaggat gcggcgggca ttgtaacggc cgaaggcggt
      241 accttccata tgcacaaagg caaagggatg gtgcgcgaag tgttccgcac gcgcaatatg
      301 cgtgatttga ccggcaacgc cggcatcgcc cacgtccgtt atcccacggc gggcaacgcc
      361 ggcagcagcg cggaggcgca gcctttctac gtcagctcgc cgttcggcat cgttttggcg
      421 cacaacggca acctcaccaa cactgccgaa ctttatgaaa acgtgtgcaa caaacaccta
      481 cgccacgtca acaccagctc cgattccgaa gtcttgctca acgtattcgc gcacgaattg
      541 cgccgcgaag tctctaaaaa cgccgacccg caccggctca atgccgacaa tattttcaac
      601 gccgttgccg aagtccaccg cctggtgcgc ggcgcatacg gcgtggttgc catgattgcg
      661 ggctacggta tgcttgcctt ccgcgaccct tacggcatcc gcccgctggt attgggttcg
      721 caaaccgaca gcgaaggcag aaaatcctat gccgtcgcct ccgaatccgt cgccttcaac
      781 gcgcttacct acgatttgga acgcgacatc cggccgggcg aagcggtatt tgtcggcttt
      841 gacggcacaa tgattgcccg ccaatgcagc gaccgtgcca aactcagccc ctgccttttt
      901 gaatatgttt attttgcccg ccccgactct gtgattgacg gcgtatccgt ttaccaatcg
      961 cgcttggata tgggcgtgtc gctggcggaa aaaatcaaac gcgagctgcc cgtggacggc
     1021 atcgacgttg tgatgcccat ccccgacacc agccgcccca gcgcgatgga gcttgccgtc
     1081 catctcgaca aaccctaccg cgagggtttg attaaaaacc gctatatcgg ccgcaccttt
     1141 attatgcccg gacaggcgac gcgcaaaaaa tccgtgcgcc aaaaactcag cccgatggaa
     1201 accgaatttg caggcaaaag cgtgttgctg gtggacgact ccatcgtgcg cgggacgacc
     1261 agccgcgaaa tcgtcgaaat ggtacgcgcg gcgggcgcgc gcaaagtcta tatcgcctcc
     1321 gccgcgcccg aagtgcgcta tcccaatgtg tacggcattg atatgcccac gcgcgaagag
     1381 ttgattgcca acgagcgcag cgcggcggaa atcgccgccg aaatcggcgc agacggcatc
     1441 gtgttccaaa atttgagcga tttggaagcc gtcgtcaaag cactcaatcc gaaaatcgaa
     1501 tccttcgatt cgtcctgttt caacggcatt tatcagaccg gcgacatcga tgatgcctac
     1561 ctcgaccgcc tgtctgccga gaaatccggc tgcggcggtc tgaaaatcca cccgagcagg
     1621 atggaacaca gcatcagcat cagcgatgcg ggcgacgaag aataaggctt cccaaaccgc
     1681 gatgccgtct gaaatccgaa cgccgggttt tcagacggca tttcccatat cggcatcaca
     1741 tttcccgttt catccgcaca caatatgagt accgaaacca aaaactacat tacgcccgcc
     1801 ggatggcagg cattgaaaga cgagctttat caactggtca acaaagaacg ccccgaaatc
     1861 gtccaaatcg tcaactgggc ggcaggcaac ggcgaccgca gcgaaaacgg cgactatctt
     1921 tacggcaaac gccgtatgcg cgaaatcgac cgccgcatcc gtttcctgac caagcgtttg
     1981 gagtccgccg tcgtcgtcga tcccgaattg cgcgaagcga ccgaccaagt atttttcggc
     2041 gcgaccgtcg gcctgttgcg cggcgacggg cgcgagcaaa ccgtcaaaat cgtcggcatc
     2101 gatgaaatcg ataccgcgca aaacaaaatt tcttggattt cgccgttggc gcgttgcctg
     2161 attaaagcgc gcgaaggcga cgaagtggtt ttgaacacgc cggaagggcg tgaggaaatt
     2221 gaaatccttt ctgtcgaata catcaaaatc gactgatcgg gaatgccgtc tgaaacggtg
     2281 caaacgcatc gtttcccaaa taaaggaata ccgaatgagg aaaatccgca ccaaaatctg
     2341 cggcatcacc acaccggaag acgcatcggc cgccgtagcg gcaggtgcag atgccgtcgg
     2401 gctggtcttt ttccaaggca gcagccgggc cgtcgatatt gcccgcgcca aaaaaatcac
     2461 cgccgcactg ccgccgtttg tcagcgttgt cgcccttttc gtcaacgaaa gcgcgcaaaa
     2521 catccgccgc atccttgccg aagtgccgat acacatcatc caattccacg gcgacgaaga
     2581 cgacacattc tgccgccagt tcgaccgccc ctatatcaag gccattcgtg ttcagacggc
     2641 atcagacatc cgaaacgccg ccgaccgctt ccccgacgct caggcactgc tgttcgatgc
     2701 ctaccaccct tcggaatacg gcggcaccgg acaccgcttc gactggacgc tgctggcgga
     2761 atattcgggc aaaccgtggg tgcttgccgg cgggctgacc cctgaaaacg tcggcgaagc
     2821 cgtccgcatc accggagcgg aagcggtcga tgtatccggc ggcgtggaag cgtctaaagg
     2881 caaaaaagac ccagccaaag tcgccgcctt tatcgcaacc gccaaccgcc tatcccgtta
     2941 aagcaacaaa aattgccgcc ggaatga
//
LOCUS       DAWEWP010000192         2938 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWEWP010000192 DAWEWP010000000
VERSION     DAWEWP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.192"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(127..1437)
                     /locus_tag="WGN53_001887"
     CDS             complement(127..1437)
                     /locus_tag="WGN53_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ0140829.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYKQMRRFVAENSSDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            1749..2171
                     /locus_tag="WGN53_001888"
     CDS             1749..2171
                     /locus_tag="WGN53_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140830.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            2257..2754
                     /locus_tag="WGN53_001889"
     CDS             2257..2754
                     /locus_tag="WGN53_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HEZ0140831.1"
                     /translation="MNVKVHKDTHHFGIHNLNNTDVHLTAEDSDNVIEIKGELADLMP
                     TGNVESTSFLRGIETAFKSNTVLTGNNNKISIATEGKGLGLGVNGLQSNSPYSGRTTG
                     IFINNQSSDLTQPSLDGISTIKLKAVKGNNEVDLNVKNHASVKGIIASHSAKATLEAE
                     GDNIV"
     gene            2859..2938
                     /locus_tag="WGN53_001890"
                     /pseudo
     CDS             2859..2938
                     /locus_tag="WGN53_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          815 a          705 c          697 g          721 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagcc
      241 gtcgggcata tagctttcgc cggcggcgta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcattaa cttggtcggg gcgttgcgaa gatggacagg
      361 cacttcgtcg ctgctgtttt ctgcaacaaa tcggcgcatt tgtttatacg ctttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaaggta caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt ggaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcggtta agtttttcag
      781 acgacgtgta tcggcggcgc gtaaaagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaagggttg
      961 caaaacatac acctgagcgc ggctcaacag cgcgggattg acttcaaacg acggattttc
     1021 cgtcgtcgca ccaataaagg ttagcaaacc gctttcgaca tgcggcaaaa acgcgtcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc gtccctgctg
     1141 caaagcgatt tcggctttat cgattgcctc gcggatgtcc ttcacgccgg aaaatacggc
     1201 ggaaacaggc aaaaactggg cgttgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccgcctt ctaccgccac
     1321 gcgcaaaggt ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcatcggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gattcaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aagagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 gtgaaaactt tagaaaatta gaacttatta ccactggtaa aaatgataat ggaaatttaa
     1921 cggtaaatgg aatttacggc atccaatctt ttgcagactc tcaattggga tatacgcctg
     1981 aattattggt aaaaagcaat aatgatattc tatttaaaaa taatctccaa tctgttgacg
     2041 tgaaacccca ttccggaaat atccatttgg atgccaaagg aaaaattcaa tttaatttga
     2101 atgttcccga tgttgatggg gtacaagatg tagccagtgg aaaaaaatac cgacaaacaa
     2161 tagtgcctta gtgcggtatg tcaacactat cagccccccg atgaaaatga caaaggcagc
     2221 atatcctttc attcaaaaac aggcaatttg ttcaatatga acgttaaagt tcataaagac
     2281 acacaccatt tcggtattca caacctcaac aataccgatg ttcatctgac tgccgaagac
     2341 agcgataatg tgattgagat taaaggggaa ctcgccgacc ttatgccgac aggaaatgtt
     2401 gagagtacaa gttttttaag gggcattgaa acggctttta aatccaatac cgtattaacg
     2461 gggaataaca ataaaatatc catcgccact gagggcaagg gcttgggatt gggcgtaaat
     2521 ggtctccagt ccaactcccc ctattccgga cgcacgacgg gcattttcat caataatcaa
     2581 tcaagcgacc ttacccaacc ttcccttgat ggcatcagca ctatcaagct caaagccgtc
     2641 aagggaaata atgaagttga tttaaatgta aaaaaccacg cctccgtaaa aggcattatc
     2701 gcgtcgcatt ctgcaaaagc aacgcttgaa gccgaagggg acaatattgt ctaggttaaa
     2761 aatccggaca ccaatacggc tttaatcgat gctttgaaaa aacaataccc caatgcttcg
     2821 aaagaactat acagggtggg ggttcaatcc acgactggaa cagtacgcat ttcgccaaac
     2881 ggcaaaatcg cattaacgga attgaaaact tttggaattg tacaaaacgg catttgcg
//
LOCUS       DAWEWP010000193         2896 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWEWP010000193 DAWEWP010000000
VERSION     DAWEWP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.193"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(227..1861)
                     /gene="groL"
                     /locus_tag="WGN53_001891"
     CDS             complement(227..1861)
                     /gene="groL"
                     /locus_tag="WGN53_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ0140832.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLEDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGMGGMM"
     gene            complement(1954..2244)
                     /locus_tag="WGN53_001892"
     CDS             complement(1954..2244)
                     /locus_tag="WGN53_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ0140833.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            2472..2896
                     /locus_tag="WGN53_001893"
                     /pseudo
     CDS             2472..2896
                     /locus_tag="WGN53_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          601 a          751 c          765 g          779 t
ORIGIN      
        1 ttcgcgggaa tgacggcgga aaggttgctg tttttccgat aaattcctgc cgctcttcgt
       61 ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca aaaaataaca atgcaaagaa
      121 cgatgttggc aattttaagg tttgcgcctt agccgcactt attcgcaaaa aaaccgcacg
      181 gcgttgaccg tgcggcttgt tgtctgaagg cttcagacgg cattgcttac atcatgccgc
      241 ccataccacc catgccgccc atatcaggca tagccggttt gtcttcaggg atttcagcaa
      301 tcatgcagtc tgtggtcagc atcaggccgg cgatagacgc ggcgtgttgc agcgcggaac
      361 gggttacttt ggcggggtcg agtacgccca tttcgatcat gtcgccgtat tcgccgctgc
      421 cagcgttgta accgtagttg cctttgcctt ccaacacttt gttcacaacc acgctaggtt
      481 cgccgcctgc gttggcaacg atttggcgca gcggagactc aacggcgcgc aagacgattt
      541 gtacgcctgc gtcttggtcg gcattgccgg tgtgcaggtt ttccaaagcg gcacgggcgc
      601 gcaacagggc tacgccgccg cctgcgacca cgccttcttc aacggctgcg cgggtagcgt
      661 gcagcgcgtc ttccacgcgg tcttttttct ctttcatttc gacttcggta gcggcgccga
      721 ctttgatcac tgccacgccg cctgccaatt tagccacgcg ctcttgcagt ttttctttgt
      781 cgtaatcgct ggttgcggtt tcgatttgtt ggcggatttc ggcaacacgc gcttcgattt
      841 gggctgcgtc gccaaagccg tcgatgatgg tggtgttttc tttaccgatt tcgatgcgtt
      901 tggcttgacc caagtcttcc aaagtcgctt tttccagaga caggccgact tcttcggaaa
      961 tcaccacgcc gccggtcagg atagcgatgt cttgcagcat cgctttgcgg cggtcgccga
     1021 agcccggagc tttgacggca acggttttca ggatgcctcg gatgttgttc accaccaaag
     1081 tcgccaaggc ttcgccttct acgtcttcag cgataatcaa cagcggacgg ctggctttcg
     1141 ccacttgttc caaaacaggc agcaggtcgc ggatgttgct gatttttttg tcaaacagca
     1201 atacaaacgg attgtccaaa gcagcgattt gtttttcagc atcgttgatg aagtaagggg
     1261 acaggtagcc gcggtcgaac tgcatacctt caaccacgtc cagctcgttt tccaaagatt
     1321 tgccgtcttc aacggtaatc acgccttctt tgccgacttt ttccatcgct tcggcgataa
     1381 tcgcgccgac ttgttcgtca gagttggcgg aaatcgagcc gacttgggcg atttctttgg
     1441 aagtatcgca aggtttggcg atgtttttca gctcgtcaac caaagcggcg acggctttgt
     1501 cgataccgcg tttcaggtcg gtcggattca tacctgcggt aacatatttc atgccttcgg
     1561 caacgatgga ttgtgccagt acggtggcgg tagtcgtacc gtcgcccgcc acgtcgttgg
     1621 ttttggacgc aacttctttc accatttgcg cgcccatatt ttcaaacttg tctttcagtt
     1681 cgatttcttt ggcgacggtt acgccgtctt tggtgatgtg cgggccgccg aaagcgcggt
     1741 caaccactac gttgcggcct ttgggaccca gggttacgcg cacggcgttt gccaaaatgt
     1801 tcacgccgtt taccattttt tggcggactt cattgccgaa ctgtacgtct tttgctgcca
     1861 tttcaattct ccaaaaatca ttaaaactgt ctgataaaac cgtttatgcc gtctgaaggc
     1921 ggtttgccgt ttcagacggc atcgtgtcca tatttatttt tcaacgatgc cgaagatgtc
     1981 ttcttcgcgc atcaccaaca gctcttcgcc gtcggctttg acggtttggc cgctgtattt
     2041 gccgaagatg attttgtcgc cgactttgac atccagcgga cggcgcgcgc cgtctttacc
     2101 gattttgccc gcgcccacgg cgatgacttc gcccatatcg ggtttttcgg cggccgcacc
     2161 cggcaaaacg atacccgatg cggttttttc ttcagcttcc aagcgtttga cgacgacgcg
     2221 gtcgtgtaaa ggacggatgg tcatatttat gctccgataa aatggtttga aaacaatcat
     2281 ctgcccgaac ggttcggaca gattgaagtg gaaaccgggc tgccgcctgc cggccgcccg
     2341 tatgaatcgg caaaattagg gtgtgtgcgg gcaaattcaa gtgaggcgga aaaaatttat
     2401 ttccgccgtt ttttatagtg gactaaattt aaggggctgt actagattag cagatatgtt
     2461 accctcgaaa tatgaagata acgcactgca aatgaaagaa aaaagtacag aaagaactgc
     2521 tccgtttttt tgtactcgaa gttaccgccc gttctgccgc cgatattttg ggtatccatc
     2581 ccaattcggc agcactgttc taccgtaaaa tccgcatggt tatcaaccat catttggcct
     2641 tggctgccga tgaggttttt gagggctctg tcgagccgga cgaaagcgat ttcggcggac
     2701 ggcgtaaagg cagacgtggt cgcggtgcgg caggaaaagt ggttgtcttc ggcattctga
     2761 aacgcaacgg acgggtctat accgttgtgg tggataatgc caagtctgaa acgttactcc
     2821 ctgtcatcaa aaagaaaatc atgccggaca gtattgttta taccgatagt ctgagcagct
     2881 gcgacaagtt ggacgt
//
LOCUS       DAWEWP010000194         2869 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWEWP010000194 DAWEWP010000000
VERSION     DAWEWP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.194"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..388
                     /locus_tag="WGN53_001894"
     CDS             <1..388
                     /locus_tag="WGN53_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140834.1"
                     /translation="LKQYACDGIKFYGYIPYAEMMSVAKGCDISVNAIHSYAMQSITN
                     KLSDYMALQKPILNSQVNDEVAEVLTLLPHANYRSSDVDSFVKAAKDILKRKDDPVQS
                     EEIVRRFKRDVAYQKIVNLIERLAHE"
     gene            381..998
                     /locus_tag="WGN53_001895"
     CDS             381..998
                     /locus_tag="WGN53_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046551259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEZ0140835.1"
                     /translation="MNKFFKRLFDIVASASGLIILSPVFLILVYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMIKFRSMRDAIDKDGNPLPDSERLTDFGKKLRATSLDELPELWNVLK
                     GEMSLVGPRPLLMHYLPLYNNFQNRRHEMKPGVTGWAQVNGRNAISWDEKFAHDVWYI
                     DHFSFWLDIKILFLTVKKVFVKEGISAEGEATMPYFTGNKTDEKK"
     gene            985..1959
                     /locus_tag="WGN53_001896"
     CDS             985..1959
                     /locus_tag="WGN53_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ0140836.1"
                     /translation="MKKNNILILSAGRRVELVQDFQTEAARFSDGIGVFATDLNPHMS
                     SACHVADRAFSVPRIDAAEYIDSILELAQQHDIGLIVPTIDTELLKLAEARDRFEAEG
                     IHIVISDAKLITLCCDKRLTAGLFAQYSIRSPEIYERDRLVFPCFAKPYDGSRAIGAK
                     KINTPADLTVDITEDPKMMFAQYIDIENTFSEFTVDMYYDRQGRLKCAIPRKRLEVRS
                     GEISKGVTCKNSLYQTLLEKMSVLQGARGCITAQFFYNEETGEFYGVEINPRFGGGFP
                     LTYAAGGNYPSRLMREYLGDEEIPFSDDWENNLIMLRYDAKVLVHEND"
     gene            1946..2632
                     /locus_tag="WGN53_001897"
     CDS             1946..2632
                     /locus_tag="WGN53_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0140837.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDSKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSFQ
                     DLVPIIDSNT"
BASE COUNT          746 a          802 c          658 g          663 t
ORIGIN      
        1 gctcaaacaa tatgcctgcg acggcatcaa gttttacggc tacattcctt acgccgaaat
       61 gatgtcggtc gccaaaggct gcgacatttc cgtcaacgcc atccactctt acgccatgca
      121 gtcgattacc aacaaactgt ccgactatat ggctttgcaa aaaccgattc tgaacagcca
      181 ggtcaacgac gaagttgccg aagtcctcac cctgctgccg catgcaaact accgttccag
      241 cgatgtggac agcttcgtta aagccgccaa agatattttg aagcgcaaag acgatcctgt
      301 tcagtccgaa gaaatcgtcc gccgcttcaa gcgcgacgtt gcctatcaaa aaatcgtcaa
      361 cctgattgaa agattagctc atgaataaat ttttcaaacg cctgtttgac attgttgcct
      421 ccgcctcagg gctgattatc ctgtcgcctg tgtttttgat tttggtgtac ctcatccgca
      481 aaaacttagg ctcgcccgtg ttcttcatcc aagagcggcc gggcaaggat ggcaagccgt
      541 ttaaaatgat taaattccgc tccatgcgcg atgcaatcga taaagacggc aacccgctac
      601 cggacagcga acgcctgacc gatttcggca aaaaattacg tgccaccagc ttggacgaac
      661 ttcctgaatt atggaatgtc ttaaaaggcg agatgagtct ggttggccct cgtccgctgc
      721 tgatgcacta cctgccgttg tacaacaact tccaaaaccg ccgccacgaa atgaaaccgg
      781 gcgtaaccgg ttgggcgcaa gtcaatggcc gcaacgctat ttcatgggat gaaaagttcg
      841 cccacgatgt ttggtacatc gaccacttca gcttctggct ggatattaaa attttgttcc
      901 tgacggttaa aaaagtcttc gtcaaagaag gcatctcagc cgaaggagaa gccactatgc
      961 cttatttcac gggtaataaa actgatgaaa aaaaataaca tactgattct gtcggcaggc
     1021 cgccgcgtcg aattggtgca ggacttccaa accgaagccg cacgtttttc agacggcatc
     1081 ggcgtttttg ccactgacct caacccacac atgtcttctg cctgccatgt tgccgaccgc
     1141 gctttcagcg tgccgcgtat cgatgcagcc gaatacattg acagcattct cgagctggcc
     1201 caacaacacg acatcggtct gattgtgcca accatcgata ccgagctgct caaactggcc
     1261 gaagcacgtg accgttttga agccgaagga atacatattg tcatttccga tgcaaagctg
     1321 attaccctgt gctgcgacaa gcgcctgacc gcaggcctgt ttgcacaata cagtatccgt
     1381 tcgcctgaaa tttacgaacg ggaccgcctg gttttcccat gttttgccaa gccttatgac
     1441 ggcagccgcg ccatcggtgc gaaaaaaatc aacacaccgg ctgatttgac tgtcgacatt
     1501 actgaagacc cgaaaatgat gttcgcccaa tacatcgata tcgagaatac tttctccgaa
     1561 tttaccgtcg atatgtatta cgaccgtcaa ggccgtctga aatgcgccat cccgcgaaaa
     1621 cgcttggaag tgcgctccgg cgaaatcagc aaaggcgtta cctgcaaaaa cagcctctac
     1681 caaacgctgc tggaaaaaat gtcggtattg cagggcgcgc gcggctgtat cacggcgcag
     1741 ttcttctaca acgaagaaac cggcgaattc tacggcgtgg aaatcaaccc ccgcttcggc
     1801 ggtggcttcc cgctgacgta tgccgcaggc ggcaattatc cgagccggtt gatgcgcgaa
     1861 tacctcggcg acgaagaaat cccgttttca gacgactggg aaaacaacct cattatgttg
     1921 cgctacgatg ccaaggtctt ggttcatgaa aacgattaat cctgaaacca ccgtcatcgt
     1981 cttcgacttg gacgataccc tgtattcgga atacgaatac aagctctccg gtatccgttc
     2041 ggtcatcgat accgttgccg cactgtatcc cgattggaat tcagacaacc tatggcgcaa
     2101 catcgacccc gacggcaaag attggctgga caaattgtgc cgccactgtg gttttaatga
     2161 atcggaaaaa caggttcttt tgtggcaata ccggctgcac cgcccgacgc tgactccgta
     2221 tgctccgccg gatttcctgt ccgaactgac cgcgcccttc gccgcacgcg cgctgattac
     2281 cgacggcaga agcctgaccc aacgcctgaa gctggaagcc ttgggtctgt cttccctgtt
     2341 tgacgacatc ctcatctctg aagcctgctc ttcggaaaag cccgacagca aacgcttccg
     2401 ccatcttcaa gacaaatatg ccgacaaggc aggctgcttc atctatatcg gcgacaacat
     2461 ttccaaagac tttatcgcgc cgaacacttt gggctggata accataggat tgcttccctc
     2521 cggacagaac atccaccgcc accagcctga aagttttaac acagaatatc atcccgactt
     2581 ttgggttaac tcctttcaag atttagttcc tataattgat tcaaatactt aaccattaca
     2641 taatcaaaca tgcactcaca atattaaaag gactatttca tgacaaaata ttattcatgg
     2701 ggcgtaaacg gctttccgcc ctacgttttc gacaccattt ggaaacgatg gaagttgaat
     2761 gggcgcgggg gcggttatcg tgtgcgacat cccggacggc atgaccgtcg cgggcaatcc
     2821 ggcaaagccg ctgccgcgca aaaaccccaa gaccgggacg gcataagcg
//
LOCUS       DAWEWP010000195         2827 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWEWP010000195 DAWEWP010000000
VERSION     DAWEWP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.195"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1..525
                     /locus_tag="WGN53_001898"
                     /pseudo
     CDS             1..525
                     /locus_tag="WGN53_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(572..1981)
                     /gene="dacB"
                     /locus_tag="WGN53_001899"
     CDS             complement(572..1981)
                     /gene="dacB"
                     /locus_tag="WGN53_001899"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ0140838.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDIADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            2146..2712
                     /locus_tag="WGN53_001900"
     CDS             2146..2712
                     /locus_tag="WGN53_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0140839.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          688 a          658 c          771 g          710 t
ORIGIN      
        1 ttacgattac ttatttatca aaacagccca catttagaaa tgtttgatgg cgaagtagaa
       61 gcagatgaaa gttattttgg cggacaacgc aaaggcaaac gtggtcgcgg tgctgccggt
      121 aaagtcgccg tattcggtct tttgaagcga aatggtaagg tttatacggt tacagtaccg
      181 aatactcaaa ccgctacttt atttcctatt atccgtgaac aagtgaaacc tgacagcatt
      241 ttttgtagct atgatgtatt agatgtgcgc gaatttagcc attttagctt cgctgaaact
      301 tcgttttcgt atcaatcaca gcacacattt tgccgaacga caaaaccata ttaatggaat
      361 tgagaacttt tggaatcaag caaaacgtca tttacgcaag tttaacggca ttcccaaagc
      421 gcattttgag ctgtatttaa aggagtgcga atggcgtttt aacaacagtg agataaaagt
      481 tcttgttcca tcttaaaaca attagtaaaa ccgagtttat cctagttatc taggacagcc
      541 ccttaaattt aatccactat attttcctgt ttcaggctcg gcgttctttg cacatcagtt
      601 tcgcatccag ccagccgtcg ccgccggaga tgatgttttt ggcaacgaag ttgtccaagt
      661 cgggcagcag ggaaacggcg cggccgctgt tgatgatgac gaccaccgcc atcggtttgt
      721 cgcccagcca ataacctgca agggcgcgga cattgttgag cgtgccggtt tttaagcgca
      781 acagcccgcc gctttgtttg aagcggttgc gtaaagttcc gtctgtgccg gcgatgggca
      841 gcgtgtcgat gaaatcttgt gcaaacgggc tgaaataagc cgtttccaac atttgcgcca
      901 tcattctcgc cgttaccctt tcttttctgg acagacctga accgttttcc aaaaccaaat
      961 ccgcaatatc gatgcccgac acggcaagtt cgcgccggac ggcagacgcc gcctgttcgg
     1021 aaacggcggg cagtttgccg tcgccgccga gtttgaggaa gacggaacgc gcaattagat
     1081 tgtccgaacg cttgttcatg tccgtcaaaa tttccttcat cggctttgag tgtgcaacgg
     1141 caagcgtctg cgcgccttcc ggcgtgtcgg atatgccgat gccgtctgaa atccgtccgc
     1201 cgccgagcag ccagtggttg gtaaaacttt gccggatcag ttcgtcaagc gcgaacatcc
     1261 ggacaccgac aggcttgccc aaacagctct cgggaatatt gccgcgcaat ttcagcgtat
     1321 tgtccgaaaa agatgcacgc atcagttttt tgatcgaagg gcaggcagct tgggaggcgg
     1381 taattttcaa gttgttttgg gcgaaaatat gcggcaaagg cggatcggtg aggatgtcgg
     1441 tactgtcggc ggcattgcgt tcggcgcgca ccataaccat accggcagac agcatagttg
     1501 gattgggggg cgtcataaac ggcgaaccgc tgtcggcttc gaaatcgtcg gggctgccga
     1561 cttcgcccca caggctgtgg tcgagcatca ggtgtcccgt gatattgcgt atgccttgtt
     1621 cgcgcaactg gcgttggacg gcaagcaggt tttcctgatt gaaaacgggg tcgccgctgc
     1681 ccgcccaata caggtttccg tcaagcgtgc cgtcgtttac cgtaccgttg cttttaaact
     1741 cggtcgccca gcggtaattg ctgccgaagg ttttgaaggc ggcaaacgcg gtaacgagtt
     1801 tcattgtgga ggcggggttg acggggacat ccgagcggtg gtcaatgatg acttttccgc
     1861 tgtcaagctc ttggacatat acggcgattt cgttttgcgg aatgcgacct gtatcgagcg
     1921 cgtgtgcggc gagggaggcg agaagcagca gcagggaggc cgctgttttg gggaaattca
     1981 taggtaaatc tgttacataa aaaaagcaat tataaggcaa agccggataa gtgggaacgc
     2041 tgtggcggcg ggccggcttg tttgcagggg aaatcacata tataataatc gctatcatta
     2101 tgaaatgatt gagacgcaca aacttaatat tcaggaggaa tgattatggc taagaaaatc
     2161 agtattttgg tgggcagcct gcgccgtgct tcttttgcgc gcaaagtggc attgaatgcg
     2221 gcggagatgt tccccgaagg ctggcaggcg gaaatcgtcg aaatcggaca tctgccgctt
     2281 tacaatttcg attatgacga ccctgcggtg gaagatgtgc cgctgcccga aagctacacg
     2341 gttttccgcg aaacgattaa ggcttcggac ggcattttgt tcgttacgtc cgaaaataac
     2401 cgcaccattc ccgcctgttt gaaaaatgcg gtggacatcg gctcgaaacc gaatgccgac
     2461 gtggcgtgga aaaacaaacc ggccggcatc atcagccatt ccgtcggcaa gatgggcggt
     2521 tacagttcgc aaaaaaacct gcgccttgcg ctgtcttatt tcgatatgcc cgtaaccgga
     2581 cagccggaag tgtttttggg caattcgccg acactgtttg atgaaaacgg caaattgatc
     2641 gactcggcaa gggattttgt tcagtcatat attaatcagt ttgtcggttt gattgaaaga
     2701 aatgccaaat aaacaaacaa gtcgaaaggc cgcaaaaccg gttcgggttt tgcggctttt
     2761 ttatatgggc tgggcagcgt caaggtttgc cgtttgccgg ataatgcgcg gcaaatgacc
     2821 ggcgtgt
//
LOCUS       DAWEWP010000196         2627 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWEWP010000196 DAWEWP010000000
VERSION     DAWEWP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.196"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            121..750
                     /locus_tag="WGN53_001901"
     CDS             121..750
                     /locus_tag="WGN53_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ0140840.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPILLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            896..2557
                     /locus_tag="WGN53_001902"
     CDS             896..2557
                     /locus_tag="WGN53_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ0140841.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGHADFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT          514 a          667 c          684 g          762 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt taatccacta tattttgacg gggttgaagt
       61 tgacatctac aaggtttaat gtttgaatac atttaaaaat atatttgaat gagatgtttt
      121 atgattcaaa atgtcgttac ttcaataatc ctgtattctg ggacagccgt agacttactt
      181 attatcctaa tgttattttt tgccaaaaga aaaagtagaa aagacatcat taacatctat
      241 ttaggacaat ttctaggctc tgttagtctg atattgctaa gtttactttt tgcatttgtc
      301 ttagattata ttcctagtaa agagatttta ggtttgctcg gcttgattcc aattctccta
      361 ggcatcaaag ttttgctttt aggagattct gatggagagg ctattgccaa agagggtttg
      421 cgcaaagata ataaaaacct gatttttcta gtcgctatga ttacttttgc aagttgtggt
      481 gctgacaata ttggtgtctt tgtcccatat tttactacct taaatttagc gaatttgata
      541 gtggctttac ttacctttct agtcatgatt tatctcttgg ttttttctgc ccaaaaatta
      601 gcacaagtcc cttctgttgg agaaactttg gaaaaatata gcagatggtt tgttgctgtt
      661 gtttatttgg gattgggggt atatatcctg attgaaaaca acagttttga tatgctatgg
      721 actgtgtcgg gccaggaaaa aatattatga aaaagatagt atctgcaaag actgccatgt
      781 gcagtctttt ttgttgccgg tctttttgtg tctgatgccg tctgaagcag tctctgcacg
      841 acctttgtgc gaatatttgc tacacttggc aatccgtcca ttttttctca cgcttatgct
      901 gacctacact ccgcccgatg cccgcccgcc cgccaaaacc cacgaaaagc cgtggctgct
      961 gctgttgatg gcgtttgcct ggttgtggcc cggcgtgttt tcccacgatt tgtggaatcc
     1021 tgccgaacct gccgtctata ccgccgtcga agcactggca ggcagcccca cccccttggt
     1081 tgcccatctg ttcgggcatg ccgatttcgg cataccaccc gtgtatcttt gggttgccgc
     1141 cgcgttcaaa catttgctgt cgccgtgggc agccgacccg tatgatgccg cacgctttgc
     1201 aggcgtgttt ttcgccgttg tcgggctgac ttcctgcggc tttgctggct tcaacttttt
     1261 gggcagacac cacgggcgca gcgtcgtcct gattctcatc ggctgtatcg ggctgattcc
     1321 gaccgtacac tttctcaacc ccgctgccgc cgcctttgcc gccgccggac tggtgctgca
     1381 cggttattct ttggctcgcc ggcgcgtgat tgccgcctct tttctgctcg gtacgggctg
     1441 gacgctgatg tcgttggcag cagcttatcc ggcagcattt gccctgatgc tgcccttgcc
     1501 cgtactgatg tttttccgtc cgtggcaaag caggcgtttg atgttgacgg cagtcgcctc
     1561 acttgccttt gccctgccgc ttatgaccgt ttacccgctg ctcttggcaa aaacgcagcc
     1621 cgcgctgttc gcgcaatggc tcgacgatca cgttttcggt acgttcggcg gcgtgcggca
     1681 cgttcagacg gcattcagtt tgttttacta tctgaaaaac ctgctttggt ttgcactgcc
     1741 cgcgctgccg ctggcggttt ggacggtttg ccgcacgcgc ctgttttcga ccgactgggg
     1801 gattttgggc gtcgtctgga tgcttgccgt tttggtgctg cttgccgtca atccgcagcg
     1861 ttttcaggat aacctcgtct ggctgcttcc gccgcttgcc ctgttcggcg cggcgcaact
     1921 ggacagcctg aggcgcggcg cggcggcgtt tgtcaactgg ttcggcatta tggcgttcgg
     1981 actgtttgcc gtgttcctgt ggacgggctt tttcgccatg aattacggct ggcccgccaa
     2041 gcttgccgaa cgcgccgcct acttcagccc gtattacgtt cccgacatcg atcccattcc
     2101 gatggcggtt gccgtactgt tcacaccctt gtggctgtgg gcgattaccc gcaaaaacat
     2161 acgcggcagg caggcggtta ccaactgggc ggcaggcgtt accctgacct gggctttgct
     2221 gatgacgctg ttcctgccgt ggctggacgc ggcgaaaagc cacgcgccgg tcgtccggag
     2281 tatggaggca tcgctttccc cggaattaaa acgggagctt tcagacggca tcgagtgtat
     2341 cgacataggc ggcggcgacc tgcacacgcg gattgtttgg acgcagtacg gcacattgcc
     2401 gcaccgcgtc ggcgatgtac aatgccgcta ccgtatcgtc cgcttgcccc aaaatgcgga
     2461 tgcgtcgcaa ggctggcaga cggtttggca gggtgcgcgc ccgcgcaaca aagacagtaa
     2521 gttcgcactg atacggaaaa tcggggaaaa tatataaaaa acaacagatt gagccgaatt
     2581 tctggattaa gtgccggaaa tcgcgtataa ttgcgcgatt aaacctt
//
LOCUS       DAWEWP010000197         2504 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWEWP010000197 DAWEWP010000000
VERSION     DAWEWP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.197"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(13..819)
                     /locus_tag="WGN53_001903"
     CDS             complement(13..819)
                     /locus_tag="WGN53_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ0140842.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="WGN53_001904"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="WGN53_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ0140843.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          693 a          630 c          652 g          529 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttac cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcgaaga atgtgccgct
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 aagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccgt ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccgc gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtctgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccgttt cgggcaagcc ttgacggatt tgccgatatg ctgtccggca cacaagccgc
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggccaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt cgtcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagtg gctggcagcc tgacgccgga tcaggcattt
     1261 atcggcgtgg tcaacaaagc cctgaccgaa ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttg cgccgcccgc cgtcgtgttg atggcaggtt tgcagggcgc gggcaagacg
     1381 acgaccgccg gcaaactcgc ccgcctgctg aaaaacgatc agaagaaaaa ggttttggtc
     1441 gtatctgccg acgtttaccg ccctgccgcg attgaacaac tgcgcctgtt ggccgaacag
     1501 gtcggcgtgg actttttccc atccgatacc aaccaaaaac cggttgaaat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgttttga tggtcgatac cgccggccgt
     1621 ctggcaatcg atgaagagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccgga gtcgtattga ccaagatgga cggcgactcg
     1801 cgcggcggtg cggcattgtc cgtacgccat gtaaccggca aaccgattaa atttatcggt
     1861 gtcggcgaaa aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cagccgtatc
     1921 ctcggcatgg gcgacgtatt gaccctgatt gaagacgttc aaaaaggtat tgatgaagaa
     1981 gccgccgcca aaatggcgaa aaagctgcaa aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccagcaaat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat atcgaaacaa atccccgaag gtacggctga aaaagcgatg
     2161 ggtaaagtag aagccatcat caactcgatg acccctaaag aacgcgccaa ccctgccctg
     2221 ctcaaagcca gccgcaaacg ccgtattgca atgggtgcgg gcacgaccgt acaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacaaatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaagg gaatgaaagg aatgttcccg
     2401 ggtttgtaag ccgatttgac cgaaaacgcc gtctaaaatt tcagacggcg tttttgtttt
     2461 atattctgat ttatagtgga ttaacaaaaa tcaggacaag gcgg
//
LOCUS       DAWEWP010000198         2503 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWEWP010000198 DAWEWP010000000
VERSION     DAWEWP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.198"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            168..791
                     /gene="lolA"
                     /locus_tag="WGN53_001905"
     CDS             168..791
                     /gene="lolA"
                     /locus_tag="WGN53_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ0140844.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTKPYRQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1064..2203
                     /locus_tag="WGN53_001906"
     CDS             1064..2203
                     /locus_tag="WGN53_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEZ0140845.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEQETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2336..>2503
                     /locus_tag="WGN53_001907"
     CDS             2336..>2503
                     /locus_tag="WGN53_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0140846.1"
                     /translation="MNLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKI"
BASE COUNT          682 a          731 c          619 g          471 t
ORIGIN      
        1 gcggcacaca ttaaagggca gcgtggcgcg cctgcctttt ccggtgggca aaaaatcggc
       61 cctcggaaaa cgctgtttgt aaaatgcaaa ccgcccgtaa cgccgcccgt atgattgttt
      121 tgccgcgccg atactttacg ccacactcat cccgacaagg aaaaataatg atgaaaccgc
      181 acaacctgtt ccaattcctc gccgtttgct ccctgaccgt cgccgtcgct tccgcacagg
      241 cgggcgcggt agacgcgctt aagcaattca acaacgatgc cgacggtatc agcggcagct
      301 tcacccaaac cgtccaaagc aaaaagaaaa cacaaaccgc gcacggcacg ttcaaaatcc
      361 tgcgaccggg ccttttcaaa tgggaataca ccaaacctta caggcaaacc atcgtcggcg
      421 acggtcaaac cgtttggctc tacgatgttg atctggcaca agtgaccaag tcgtcccaag
      481 accaggccat aggcggcagc cccgccgcca tcctgtcgaa caaaaccgcc ctcgaaagca
      541 gctacacgct gaaagaggac ggttcgtcca acggcatcga ttatgtgctg gcaacgccca
      601 aacgcaacaa cgccggctac caatacatcc gcatcggctt caaaggcggc aacctcgccg
      661 ccatgcagct taaagacagc ttcggcaacc aaacctccat cagtttcggc ggtttgaata
      721 ccaatcccca actctcgcgc ggcgcgttca agtttacccc gcccaaaggc gtggacgtgt
      781 tgagcaactg atgccgtccg ccccgatgcc gtctgaaagc cgccgaggct tcagacggca
      841 ttttcacgca ggcggaacaa tgtcccgcat taccgcccga tcgggcatcg gaacggcaaa
      901 cccgtgaaaa ttaacggttg cgccccggtt gtttttgccg tttaatgcaa accttgctgc
      961 atcaagggcc aagaaagccg accggccgcc cccacagctt ccgatgcagg cggccaggcc
     1021 gtccctgcaa tgttttttat ttttgaacga aaggtcgaaa accatgaaaa aaacactggt
     1081 ggcggcggca atcctgagcc tcgccttgac tgcgtgcggc ggcggaagcg ataccgccgc
     1141 ccaaaccccc tccgccaagc ccgaagccga acaatcgggc aaactcaata tctacaactg
     1201 gtcggattat gtcgatcccg aaaccgtcgc cgcctttgaa caagaaaccg gcatcaagac
     1261 gcgttccgat tattacgaca gcaacgaaac actggaggca aaagtcctga ccggcaaatc
     1321 cggctacgac ctgaccgcgc cgtccatcgc caacgtcggc cggcaaatca aagcgggcgc
     1381 gtatcagaaa atcgacaagg cgcaaatccc ccattacggc aacatcgata aagatttgct
     1441 gaaaatgatg gaagccgtcg atccgggcaa cgaatacgcc gtcccctatt tctggggcat
     1501 caacaccttg gcaatcaaca cgcggcaggt gcaaaaggca ttgggtacgg acaagctgcc
     1561 cgaaaacgaa tgggatttgg tgttcaaccc cgaatacacc gccaaactca aatcctgcgg
     1621 catcagctat ttcgacagcg caatcgaaca gattcccttg gcgttgcact atttgggcaa
     1681 agaccccaac agtgaaaatc ccgaagacat caaagccgcc gtcgatatga tgaaagccgt
     1741 ccgaggcgac gtgaaacgct tcagctcttc cggctatatc gacgatatgg cggcgggcaa
     1801 cctgtgtgcc gccatcggtt acggcggcga tttgaacatt gccaaaaccc gtgccgaaga
     1861 agccgcaaac ggcgtggaaa tcaaagtatt gaccccgaaa accggcgtgg gcgtgtgggt
     1921 ggattccttt atgattccgc gcgacgcgca aaacgtcgcc aacgcccacc gctatatcga
     1981 ctacacgctc cggcccgagg tggcggcgaa aaacggcagc ttcgttacct acgcgcccgc
     2041 cagccgtccc gcgcgcgagc tgatggatga aaaatacacc tccgacgcat cgattttccc
     2101 gaacaaagaa ctgatggaaa aaagtttcat cgtatcgccc aaatccgcag aatccgccaa
     2161 actgggcgtg aagctgtggc aagggctcaa ggccggaaaa taaccgcaac ccctgccgtc
     2221 tgaaaccttt cgggcggcag gaaacggcgc gtccgttatc aaacaggggg gcgtttcccc
     2281 tcctgccggt tatgattggg ttaagattaa aatgatttag taaaatgaga aagatatgaa
     2341 tttaagtatc gtagttccca tttataatgt cgaaagttat ttggaagcgt gtttaagttc
     2401 catagaacct atattaagta atgaaaatgt cgaacttatc cttgtgaatg acgggtcaaa
     2461 agacggaagt gaagatatat gttataaata tatagataaa ata
//
LOCUS       DAWEWP010000199         2468 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWEWP010000199 DAWEWP010000000
VERSION     DAWEWP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.199"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(212..325)
                     /locus_tag="WGN53_001908"
                     /pseudo
     CDS             complement(212..325)
                     /locus_tag="WGN53_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            465..540
                     /locus_tag="WGN53_001909"
     tRNA            465..540
                     /locus_tag="WGN53_001909"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:499..501,aa:Glu,seq:ttc)
     gene            925..1090
                     /locus_tag="WGN53_001910"
                     /pseudo
     CDS             925..1090
                     /locus_tag="WGN53_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
     gene            complement(1140..1946)
                     /gene="thiD"
                     /locus_tag="WGN53_001911"
     CDS             complement(1140..1946)
                     /gene="thiD"
                     /locus_tag="WGN53_001911"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ0140847.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCKAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          595 a          636 c          623 g          614 t
ORIGIN      
        1 aagttgcact tcaaatgcga atccgccatt atctgaaagt ttgaggttct agattcccgc
       61 tttcgcggga atgacgaaaa gtggcgggaa tgacgaagtt tcagacggca tcgcccgcct
      121 gttttgatat ggcggcaccc ccccccgaca aaaaaaaaca atccggaacg catctgaccg
      181 ttccggcttg ttttcaggcg aatccgccgc atcagaacat actgcgcacg cccatattga
      241 cctgccaagt ctggcgcatc gtgtgcatcg aagacctttg cgcctcaaaa taaagctgcc
      301 ttccgttgtc ggcattacca cgcaaaaaaa tgaattgctt gatattccaa tgttttttat
      361 atgtttttat attgtgatgc gatcagacaa acgcccccct gacatttgtt tagacggcat
      421 cgtattgcta aatttctata agtatgtata atgtccgttt ccacgcgccc atcgtctaga
      481 ggcctaggac actgcccttt cacggcggca accggggttc gaatccccgt gggcgtgcca
      541 attcaaaaac ctgcttgttt caagcaggtt tttttattat gaatcgtcat tcccgcaccc
      601 ttcgtcattc ccgcgaaagc gggaatctgg agcgcaggat taaagaaacc gttttatccg
      661 ataagcttcc gtgccgacag gtctggattc ccgcctgcgc gggaaggacg gcagagggtg
      721 gacgatgccg tctaaagcct gacaaggcat ttgatgccgt ctgaaacttc gtcattcccg
      781 cacccttcgt cattcccgca aaagcgggaa tctagagcgt agggttgaag aaaccgtttt
      841 atccaataag tttccgtgcc gacaggtctg gattcccact ttcgtgggaa tgacgagtct
      901 tttataacct gagacctttg caaaatgcct ttccctttta aatttaaaga ggggggagct
      961 gaaggattat tatcgggatt gggagctggt cgaatataaa gaagaattgg gcgcgatgca
     1021 cgccaaagac gaaaacggca atccgatacg gtttaaattt gtaaccatgc tggcgaaaaa
     1081 gcctgaataa tcctcaaaaa tgccgtctga acattttgtt tcagacggca tttttacgtc
     1141 tagtcccgat acgcccaatg attgaccggg ccgtgtcccg cgccgatttc caaagggttt
     1201 gagattgccg ccgtaatgta ggacttggca gtccgtacgg ctttgcaaac gtccgagcct
     1261 tttgccaact cggcggtgat gcaggcggaa aacgtgcagc ccgtgccgtg cgtgtgggcg
     1321 gttggaaagc gcgggctgtc caattccagc gtttcatttt gcgtaaacag ccaatccgtg
     1381 cagcgtccgc ttgtgctgcc gttcaaatgt ccgcctttga taacgacatt tttgacaccg
     1441 taatcaagca ggatttttgc cgcacgttcc gcatctttac ggttttcgat atgcacgccg
     1501 gtcagggctt ccgcctcggg caggttgggg gtcaatacat ccgtatcggg aagcagcagg
     1561 cgcgtcagtg ccgcaacggc ggaatcctgc aacagcggcg caccgccttt ggcaatcatc
     1621 acagggtcga gtacgcgcct gccgaagtcg cagtgtttca gcttgtcggc aacgcattcg
     1681 atgatttccg ccgtgccgag cattcctatt ttgtaggcgc ggatgtcaaa gtcttccctg
     1741 attgcttgga tttgtgcggt gatggtttcg gtcgggacga gatgaaccgc cgacacgccc
     1801 aaggtatttt gcgcggtaac ggcggtgatg acgcacgttc cgaacacgcc gcgcatctga
     1861 aacgttttca aatccgcctg aatgcccgca ccgccgcccg aatccgaacc ggcgatggta
     1921 agcgtttgca caaaagagct tttcatttgt tcctccttaa tattgtgtcc gataaaaagg
     1981 gctaatgcct gtaaaaaagc acggcgatgc cgtctgaaaa ccgcagcgcg gggtttgtgg
     2041 tgcattatat gccgtattgc aggacaaggc acagggcagg gcggcggggt gcatcgtagc
     2101 aactgtattt ttgcccgacg gggtgaaaaa tacagttgct acacccactg ataatgaaaa
     2161 aagagagcaa tgccgtctga accgcgtgtt gcggcgagca taaaccggca cgctttattc
     2221 gtcattccca caaaaacaga aacctaaaat tccgtcattc ctacgaaagc gggaatccag
     2281 tgtgttcggt ttcagttgtt tttgggtttc gggtgatttc caaatcgaaa cctttgcaaa
     2341 attccccaaa atcccctaaa ttcccaccaa gacatttagg ggatgttcca tgagcacctt
     2401 ctttcaacaa accgcacaag ccatgattgc caaacacatc gaccgtttcc cgctattgaa
     2461 gttggacc
//
LOCUS       DAWEWP010000200         2457 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWEWP010000200 DAWEWP010000000
VERSION     DAWEWP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.200"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(122..1573)
                     /locus_tag="WGN53_001912"
     CDS             complement(122..1573)
                     /locus_tag="WGN53_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEZ0140848.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLNPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1596..2216)
                     /locus_tag="WGN53_001913"
     CDS             complement(1596..2216)
                     /locus_tag="WGN53_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0140849.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
BASE COUNT          770 a          581 c          572 g          534 t
ORIGIN      
        1 ataggaataa gaattcggga caaaatgttt aaaccatttt gtcccgactg ctgtgatgcg
       61 gtttttttga actagcagga cgttacctaa aacctgcgtt gcctgaaacc ctttaagacg
      121 gttaaatccc tctgccgtat ttgtattcta caatcatatt atcgtcggta ataacttccg
      181 ccccagccga aggttccgtc atctgaatca gcatacgaga gacaaccttt tgtgctgcag
      241 catccacggt gctgctgtca aatacgtgcc tgccgctttc cggccaaatc aaccgggaga
      301 gacgttgctt gagcagttct ttattaggga aaactaccgg ggttgccgag ccgactacca
      361 tatgcccata gcggtatgca tagggaatac tgtgtacggc ggtagcaaaa gcatgcgggc
      421 tgtgcgtggt attaaacatt acaataccat ccgggttaag gtggctttgc acctgtttta
      481 aaaattccgc actcaacagg ttggtggaat aggcacgcca gtaccaagtc gtattcatca
      541 aaatcaggtc gaatttttca tcaggatggc gacgcagcca tttcctaccg tcatccaata
      601 caatttcaac acgtttgtcc tgcaaaagcg gggcgatttg cggctcgtcc gcgataaggc
      661 tacggtatgc cggattgatt tccgcaacga tcatcgactg catttccgga atggcagaca
      721 agacgcgcgc ccacgaacct gtactcaatc caacgacgaa aatgcggcgt atgccagact
      781 tcagggaggg tagcagatag gcacgttcga tgccgttgac actattgaat acatcggtat
      841 tgtatgcgcc gtcgtataca ttcgccccat aaacaacctt atcaccatct ctatggtaaa
      901 ccgcaacaat gccgtgtttg ttttcaatca gcctatccgg acggtcagca atattttgaa
      961 agacagaatc cggcagtagg aacatgagga tgccgaacat tagggaaact gctaccgaca
     1021 ctgcattcag tcggagactt ttttggaaca gtgtacaaaa caaagggaca gcagcagaaa
     1081 tcaaacagat gagcaggtaa atctgttggg tggacaagaa atcaagtatc acaaagccga
     1141 taaggaccgg acccaatgca ctgccggcaa cgttggcgaa ataaacattg gaaacctgtc
     1201 gtccggattt gttgccatcc gtacccacat ggtgtacgag cgggaaaatc aaccctctga
     1261 cgacggcaga cagggtaatg aagataccgg cgtggtggac gaagccggaa aaacccgtca
     1321 acaaccacgc agcacccaaa atcaaaaagt cggcaatacc cgcccacaag aagcactgcc
     1381 cgataaaggg aatatcaaca aagcggctgc ggcaaatccg tttgccaaaa tacgcgccga
     1441 cggcgatacc ggtcagaaaa caggcaaggg taaatgaaaa tgcctgaggc acggactgtg
     1501 ctgcgaacga aaacatcctc acccacaaga cttctatacc caagctcaat aagccgctaa
     1561 ggaaagaaag catataaatc aaactagtat tcggtttagt gttcactata tccatccttt
     1621 ctgtaacgca gccatactga agcagcaatc agaaggttaa agcaggctgt cagcgcaatg
     1681 gtttgggaga gggtaaaaaa gacgtagaaa aattcggcgg cggcaagcga tccgagtgcc
     1741 gcacccaaag tgttgaaaaa atataaggta ccgatagact cgccaacatt atgtattttc
     1801 cggttaaaaa aacaggtcag caagggcaag gtcgcgccca tcataaaggt aggaagcagc
     1861 aataagagga aattggcagc agcgatgatg ggcaaatcag cctcaactaa aagatgcccc
     1921 aagccggaaa tcagacccct gcttaccaaa ccgaacagac cgatggatac ttcagcgatg
     1981 caaaacaggg ggatgatact tgaaggaaaa cggtcagcaa tgcgtccacc gaaatacgca
     2041 cctacaccca agccgaccat aaatacagaa ataatgacag taatcgaact caaatcgata
     2101 cctatgtgac tgaatagaag cctctgccag ctgacctggt aaatcagggc gcagaatcca
     2161 gaggcgaaaa acaccagcga caatcctttc agggttttac ttgcggtcga attcatattt
     2221 tctattttca aataggcgga actataaatg aaatgtatca atgtgtaaac atttgtatta
     2281 accgtaggaa atcaccgtat tgctcaaact gcaggccggg aaaataaact cgtaagcccg
     2341 caaacggcgt atcactgttc aaactgctgt gcggcttcac ggtgttataa aaataatggg
     2401 gctgtcctgg acaactaaga taaactcgat tttactaatt gttttaaaat ggaaatt
//
LOCUS       DAWEWP010000201         2440 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWEWP010000201 DAWEWP010000000
VERSION     DAWEWP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.201"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(425..2305)
                     /locus_tag="WGN53_001914"
     CDS             complement(425..2305)
                     /locus_tag="WGN53_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ0140850.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFSAEAIAEEYFGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKISAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAVKPGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDSE
                     FNTGEPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAIAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSIVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENDLPGLFRVQPAGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLFQQSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          497 a          589 c          722 g          632 t
ORIGIN      
        1 atttccacct gcgcgggaat gacgattcag aagttgcccg aaacctaaaa aactgaaacc
       61 gaacgagccg gatttccgct ttcgcgggaa tgacggcgta taagttcccg tgcggacaga
      121 cctatattcc cacctgcgcg ggaatgacga ttcagaagtt acacgaaact caaaaaaaac
      181 gaaaccgaac gaaccggatt cccgctttta cgggaatgac ggcgcataag ttcccgtgcg
      241 gacagaccta gattcccacc tgcgtgggaa tgacgggatt ttagattgcg ggtatttatc
      301 gggaacggcg gcttggaagt tcattgaaac ggaaaaacaa cggaaaccca aaaaaccgga
      361 ttcccgactg tgggaatgat gagattcagg tttctgtttt tgccggagtt tgccgtatcg
      421 ggcttcagac ggcattgcct gccgttgtac ccgcgggtgc gactgccttg atgtagttga
      481 gcgagacaaa ctgcttctcg gcatccaatt cggtgatttt gaacaatgcc tgtgatttgg
      541 gcagcgcgtc aaacggaata ccggtcgcgc gcgttaccag cggcaggcct tcaatgcgga
      601 cgaggtcttc tttgaggatg gtggccgtca gctcgctcgt gccttgctgt tgcagataga
      661 cgaggctcca gtaggcttcc atttggcgtt ggaagtcggc gtaggctgta taggcggcat
      721 caaagtcgcg cagcgcggcg aagagttcgg catcgctttg ttggaaaagc ggctcggcgg
      781 tgtcgtctat caggctgatc agttgctttt ggttgatgta gtcggcggcg cggcgcagcg
      841 gcgaggtaaa ccagccgtaa tgctgcacgc ccattccgat gtgcggctcg gatttggtgc
      901 tcatacgcac tttgcctgcc ggttgcacgc ggaaaaggcc gggcaggtcg ttttcatcca
      961 acatttgcgc ccaagtgctg ttggcaagaa tcatcatctc gctgaccagc gtatcgatgg
     1021 gagaaccgcg ttcgcggcgg acgatggata cgttgccttc ctcatccaat tcgatgctgt
     1081 aatcgtattg cggcgcacgg tcgggctcgt atttgccgcg tgctttttgc agggcgatgg
     1141 cgaattgata gaaccaaatc aggtcttgat ggtgggcgaa catcatttcg cccgcttcgt
     1201 ccaagcccgc ttcggcgttg aaatgcggtt cgatggcttg gatacgcagg ttttcggaga
     1261 tgttgaccgc ttcgattttg caggtcggtt cgccggtgtt gaactcgctg tccacatcga
     1321 aataaatgct gacggcaggg cggtgtgcgc ctgcatcaag gctgaacgcg gcaatccagt
     1381 tttcgggcag catcgtgatt ttgccgccgg ggaaataaac cgtgctcaag cgttccatga
     1441 tgtttttttc cattttgtcg cccggtttaa cggcaagtga cggcgcggcg atgtggatgc
     1501 cgacacgctt cgtgccgttg tccaagtcgg tcaggcttaa agcgtcgtcc acttcggtgg
     1561 tcgattcgtc gtcaatggaa aaggcggtaa cgtcggcctt gggcaggtcg ggcatttcgg
     1621 gaagggcaag gtcggggaag cctgtgcctt taggaaagta tttgatttca aacccgtctt
     1681 gcaggtattg cggaatggac gtaatgccac ccgttttttt cgccaattcg taggcggaga
     1741 ttttcagtgc gtcggcggct ttggtaaagg ctttgtaggt cagcgactgt ttgtcgggcg
     1801 cgtgcaggat ggttttcaaa tccgccgcga tttcagacgg catctcgccc cgttttaagg
     1861 cttccgccca agcgtcgatt tgcgcgtctt gctgtttttt gcgttcgatg gcggcaagcg
     1921 cttgtttcaa agtttcttcg ggcgcggctt tgaacacgcc tttggctttt ttgtagaaat
     1981 acatcggcgc ggcgtaaagc gcaatcaagg tcgccgccag ctcggttttg gtcggcgcat
     2041 gaccgaaata ttcttcggcg atggcttcgg cggaaaattc ttcttcgccg catacttccc
     2101 acaataaatc ggtgtcgatg tccgccgcct gtgcctgcgc gttttccaaa aacgccgcca
     2161 tatcgccgtc aaactcggca aagacattgt ttgccttcac tttggtgcgc ttgccgtgtt
     2221 gggtatcgac ttggtaggta gcgtcgtttt tttggatgat ggcggcgatt ttgaattggc
     2281 cggactcttc gtaaaaaata ttcatttttc ggatttttct gtggaaactc aagcgggcga
     2341 ttttagcaga ttaccgaaaa tgccgtctga aaaaaggttg ggagagggtt ggcgcgtctt
     2401 tgcggtgctt gcgttatagt ggattaacaa aaaccagtac
//
LOCUS       DAWEWP010000202         2413 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWEWP010000202 DAWEWP010000000
VERSION     DAWEWP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.202"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(4..624)
                     /locus_tag="WGN53_001915"
     CDS             complement(4..624)
                     /locus_tag="WGN53_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0140851.1"
                     /translation="MTTHHVELRNVTKRFGAQKAVNQVDLVLKAGESVGLAGYNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            complement(682..1914)
                     /locus_tag="WGN53_001916"
                     /pseudo
     CDS             complement(682..1914)
                     /locus_tag="WGN53_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            complement(1917..2342)
                     /locus_tag="WGN53_001917"
                     /pseudo
     CDS             complement(1917..2342)
                     /locus_tag="WGN53_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
BASE COUNT          521 a          626 c          661 g          605 t
ORIGIN      
        1 aatttaatcc acgataatgc ggtcggcgtg tccgtccaac tcggcaaggg cgtgggtgct
       61 gagcaatacg gtcgcgccgc gcccgttgag ttcgcgcacg acttcgtaaa acatttgtcg
      121 tgatgcaggg tcgaggccgg ttgtcggttc gtcaaacagc aggactttgg gctcgcccag
      181 cagggcttgt gccaaggcaa ggcgttggcg catcccttta gaataagtgc cgacgcggcg
      241 gtgtgccgcc tgtgaaatgc cgacgcgctc aagcagcccc cggttctgcg tgagcggctg
      301 ttttttaagt ttggcataaa aatccagcgt ttcgatgccg gtcagcgaag ggtgcagcgc
      361 aacggtttcg ggcaggtagc cgatttggct gcgaagccgc gcccccgctt tgctgccggt
      421 acgttcgccc aaaagcatca cttcgccttc ggtcggggta atcagcccga gtatcagctt
      481 cataatggtg gacttgcccg cgccgttgta tccggcaagc ccgacgcttt ctcctgcctt
      541 caaaaccaaa tcgacttggt tgacggcttt ttgcgccccg aaccgtttgg tgacattcct
      601 caattcgaca tgatgtgtgg tcatggttgt tccttttgtg ttttcagacg gcatttggat
      661 ccggatgccg tctgaagcag actagttcaa agaaccgttt tccgccctgc cccattccga
      721 ctgccgtgtt tcggcttctt tgagcaactc gtccttcatt gcctgatagc gggtttgaat
      781 tttggggaca taaggcttca tcagcggttt gctgtccacc acgccgccgg gcagaacggc
      841 gggaaactgc gcctgcgccc atttgacgat gctgattgcg agactgttca tcaagaggcg
      901 caatacgggc gcgcgccaga tgatttggtc gatgatgccg ttgggccggt acgcgctgtc
      961 gccgaagccg tcgccgttca aatcgaacgc actgttgtcg ctccaatagt tgccgtgtcc
     1021 gccctcgctc cagtcgagaa agcgcgtgct gacgtatttg acctggcttt cgttgttgat
     1081 aaaggaattg tcgtgcaggg acgtgccttc gatggcggcg gtaaagtgta tgccgatttg
     1141 gcagttttca aaatgattgg cggacagttt atcgtagttg gcattgtagg caaaaacgca
     1201 cttgcccgct ttgttgataa tgttgtcgtg aatatcggaa tagttgacat agttgagcat
     1261 gatgccttgg tcgcggctgc cgacggcgat attgtcaaac actttgagcc gctcggaaaa
     1321 catcagcaca tagcccatat tgttgcccac ggaaatattg ccgctgattt cgctgtcgtt
     1381 ggtgtacata tagtggacgg cgaaacgcaa atcgctgaag cggttgtttt tataggtgtt
     1441 gtgcgtgctg gtattggaaa aaatgccgtc gcggcctttg gaaatatcgt tgccgacgac
     1501 ctgcgcgccg ggcgcgttcc aaacggtaac gccgttgcct cgctcgttca cgcgcaaagt
     1561 cgcatcgcca acaattttgt tctcgcgcac catcgcatcg gcagaaccat gcagatatac
     1621 gccgaccgaa ttatccaaaa tattgttgtg ttcaatcagg gcgcgcgggg cggtttcttc
     1681 gagataaata ccggcatcca ttgcaggcag gctcataccg gaacgggtaa cggtcaggtt
     1741 gcggagcgtt acgtccggcg catgcacagc tatcgtacgt ccgctcctgt ctccttcgat
     1801 ggttgcagaa cggtcagcag gcccttcaat cgtaatcggt ttgtcgatat aaagtttggt
     1861 ttgatatacg ccggaagcca gtttgagcgt atcgcccgca tgggcgcggg caaatgcggg
     1921 ataccgatca gcataatcgg ttcgtgatga tcgaccagtt tcgctccggc tatcgtgccg
     1981 tcatttgcca aagccatcag cgtatcgatc ggtttgttcg aataaccgcg cgtattgacc
     2041 gcatcggtcg tgatatagac caagcccaac tgctcatcgc ctttgtaaac gcgggcaacc
     2101 ataggcttgc cttccggctt gccgtaacgg tccgcacccg gaaaaatttc cgaaggctgt
     2161 attttcgcca gaaaatcagg cagacgctcc gcataagcgg gcagtgccgc cgtcaaaaag
     2221 acaaaggcgg taaaaaacac tgttatccgc gcccgaaacg cggacatacg tttgattgaa
     2281 aaacaggaca tgactatccc cgatataccg atactcttta atattgatat tatatgaatc
     2341 atctatcggc gattttttga tgcaaattaa atttacaaat cttaaaacca atccgcatac
     2401 gggataatat agt
//
LOCUS       DAWEWP010000203         2391 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWEWP010000203 DAWEWP010000000
VERSION     DAWEWP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.203"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..332
                     /locus_tag="WGN53_001918"
     CDS             <1..332
                     /locus_tag="WGN53_001918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140852.1"
                     /translation="NPHHRHSRAGGNPKTQRNRNLSEKTETPPTVIPAKAGIQQHNAA
                     GIYRKKQKPPPTVIPAKAGIQQPKNHRNLSAKTETPTAVIPAKAGIQTRRHGNLPNKT
                     VSLDSTS"
     gene            477..2246
                     /locus_tag="WGN53_001919"
     CDS             477..2246
                     /locus_tag="WGN53_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ0140853.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQANATDTDEDEELESVVRSALVLQFMIDKEGNGEIESTGDIGWSIYYDDHNTLHG
                     ATVTLKAGDNLKIKQSGKDFTYSLKKELKDLTSVETEKLSFGANGNKVNITSDTKGLN
                     FAKETAGTNGDPTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLNAGW
                     NIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGAKTS
                     VIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTGQAD
                     KFETVTSGTKVTFASGNGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGWNLDSK
                     AVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSVSLG
                     AGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLNNRI
                     DNVDGNARAGIAQAIATAGLAQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDTGNWV
                     IKGTASGNSRGHFGTSASVGYQW"
BASE COUNT          732 a          598 c          585 g          476 t
ORIGIN      
        1 gaaaccctca ccaccgtcat tcccgcgcag gcgggaatcc aaaaacgcaa cgcaacagga
       61 atttatcaga aaaaacagaa accccaccga ccgtcattcc cgcaaaagcg ggaatccagc
      121 aacacaacgc agcaggaatt tatcggaaaa aacagaaacc cccaccgacc gtcattcccg
      181 caaaagcggg aatccagcaa ccgaaaaacc acaggaatct atcagcaaaa acagaaaccc
      241 ccaccgccgt cattcccgca aaagcgggaa tccagacccg tcggcacgga aacttaccga
      301 ataaaacagt ttccttagat tccacgtcct agattcccgc cttcgcggga atgacgagat
      361 tttaagttgg gggaatttat cagaaaaccc ccaaccccca aaaaccgggc ggatgccgca
      421 ccatccgccc ccaaaccccg atttaaccat tcaaacaaac caaaagaaaa aacaaaatga
      481 acaaaatata ccgcatcatt tggaatagtg ccctcaatgc ctgggtagtc gtatccgagc
      541 tcacacgcaa ccacaccaaa cgcgcctccg caaccgtggc gaccgccgta ttggcgacac
      601 tgctgtccgc aacggttcag gcgaatgcta ccgataccga tgaagatgaa gagttagaat
      661 ccgtagtacg ctctgctctg gtgttgcaat tcatgatcga taaagaaggc aatggagaaa
      721 tcgaatctac aggagatata ggttggagta tatattacga cgatcacaac actctacacg
      781 gcgcaaccgt taccctcaaa gccggcgaca acctgaaaat caaacaaagc ggcaaagact
      841 tcacctactc gctgaaaaaa gagctgaaag acctgaccag tgttgaaact gaaaaattat
      901 cgtttggcgc aaacggtaat aaagtcaaca tcacaagcga caccaaaggc ttgaattttg
      961 cgaaagaaac ggctgggacg aacggcgacc ccacggttca tctgaacggt atcggttcga
     1021 ctttgaccga tacgcttgcg ggttcttctg cttctcacgt tgatgcgggt aaccaaagta
     1081 cacattacac tcgtgcagca agtattaagg atgtgttgaa tgcgggttgg aatattaagg
     1141 gtgttaaaac tggctcaaca actggtcaat cagaaaatgt cgatttcgtc cgcacttacg
     1201 acacagtcga gttcttgagc gcagatacga aaacaacgac tgttaatgtg gaaagcaaag
     1261 acaacggcaa gagaaccgaa gttaaaatcg gtgcgaagac ttctgttatt aaagaaaaag
     1321 acggtaagtt ggttactggt aaaggcaaag gcgagaatgg ttcttctaca gacgaaggcg
     1381 aaggcttagt gactgcaaaa gaagtgattg atgcagtaaa caaggctggt tggagaatga
     1441 aaacaacaac cgctaatggt caaacaggtc aagctgacaa gtttgaaacc gttacatcag
     1501 gcacaaaagt aacctttgct agtggtaatg gtacaactgc gactgtaagt aaagatgatc
     1561 aaggcaacat cactgttaag tatgatgtaa atgtcggcga tgccctaaac gtcaatcagc
     1621 tgcaaaacag cggttggaat ttggattcca aagcggttgc aggttcttcg ggcaaagtca
     1681 tcagcggcaa tgtttcgccg agcaagggaa agatggatga aaccgtcaac attaatgccg
     1741 gcaacaacat cgagattacc cgcaacggca aaaatatcga catcgccact tcgatgaccc
     1801 cgcaattttc cagcgtttcg ctcggcgcgg gggcggatgc gcccacttta agcgtggatg
     1861 acgagggcgc gttgaatgtc ggcagcaagg atgccaacaa acccgtccgc attaccaatg
     1921 tcgccccggg cgttaaagag ggggatgtta caaacgtcgc acaacttaaa ggtgtggcgc
     1981 aaaacttgaa caaccgcatc gacaatgtgg acggcaacgc gcgcgcgggt atcgcccaag
     2041 cgattgcaac cgcaggtttg gctcaggcct atttgcccgg caagagtatg atggcgatcg
     2101 gcggcggtac ttatcgcggc gaagccggtt acgccatcgg ctactcgagc atttctgaca
     2161 ctgggaattg ggttatcaag ggcacggctt ccggcaattc gcgcggtcat ttcggtactt
     2221 ccgcatctgt cggttatcag tggtaagggc tttttcgcct gtctgctgtt ggggcaggcg
     2281 gaaggtttga agggaagggt ggcgatttgc cgcctgagac ctttgcaaaa ttccccaaaa
     2341 tcccctaaat tcccaccaag acatttaggg gattttccat gagcaccttc t
//
LOCUS       DAWEWP010000204         2384 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWEWP010000204 DAWEWP010000000
VERSION     DAWEWP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.204"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            27..572
                     /locus_tag="WGN53_001920"
     CDS             27..572
                     /locus_tag="WGN53_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0140854.1"
                     /translation="MGKTVTLTSGHSNTDPGAVNGSDREADLAQDMCNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            569..775
                     /locus_tag="WGN53_001921"
     CDS             569..775
                     /locus_tag="WGN53_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140855.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            805..942
                     /locus_tag="WGN53_001922"
     CDS             805..942
                     /locus_tag="WGN53_001922"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140856.1"
                     /translation="MIGLWSAKSREASEQADLAAFEFAEGELANYQEMLKRHLQTKSV
                     E"
     gene            945..1181
                     /locus_tag="WGN53_001923"
     CDS             945..1181
                     /locus_tag="WGN53_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140857.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            1153..1572
                     /locus_tag="WGN53_001924"
     CDS             1153..1572
                     /locus_tag="WGN53_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140858.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
     gene            1469..1774
                     /locus_tag="WGN53_001925"
     CDS             1469..1774
                     /locus_tag="WGN53_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140859.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(1819..1989)
                     /locus_tag="WGN53_001926"
     CDS             complement(1819..1989)
                     /locus_tag="WGN53_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ0140860.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            2002..2220
                     /locus_tag="WGN53_001927"
     CDS             2002..2220
                     /locus_tag="WGN53_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140861.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            2217..>2384
                     /locus_tag="WGN53_001928"
     CDS             2217..>2384
                     /locus_tag="WGN53_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140862.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQT"
BASE COUNT          618 a          545 c          689 g          532 t
ORIGIN      
        1 caatcaatat tttttggaga tgatgaatgg gcaaaaccgt aaccttaacc tctggacaca
       61 gcaacaccga cccgggtgcg gtcaacggca gcgaccgtga ggcggacttg gcgcaggata
      121 tgtgcaatat cgtggctgct attttgcgcg atgactacgg tttgactgtt aaaaccgacg
      181 gcacgggcaa aggcaatatg ccgctgcgtg aagcggtcaa gctgattcgc ggctcggatg
      241 tggcgattga gtttcacacc aacgctgccg tcagcaaagc ggcgacaggc atcgaagcct
      301 tgagtaccgt taaaaacaaa cgctggtgtc aggtgttgag caaagccgtt gccaagaaaa
      361 ccggctggaa actgcgcggc gaagacggct ttaaacccga caatgcgggc cagcattcgc
      421 gcctggctta tgcacaagcc ggcggcattg tgtttgagcc ttttttcatc agcaacgaca
      481 ctgatttggc cttgtttaag acgactaaat ggggcatctg ccgcgcgatt gcggacgcga
      541 ttgcgatgga attgggggcg gcaagagtat gaatattatt ggtaaattga aagaagctgc
      601 ttcctatttt cttacaaaat tgattggaga aaatcctagt aatgagcagg taaaccgcgc
      661 acttatacag atgccaaatg ttcgtccgat acacacctat ccacgcccaa atttaagaaa
      721 ctcaggcgtg gcagccgcga aacgcgcggc gcgcaaacgc aagaatcgtc gttaatcatg
      781 ggacaggttg cgttttacga aaagatgatt gggctgtggt cggccaaaag ccgtgaggca
      841 agcgaacagg cagacttggc ggcgtttgaa tttgcggagg gcgaactggc caattatcag
      901 gaaatgctga aacggcacct gcaaaccaaa agtgtggaat agcaatgcgt attttggata
      961 tttttaaaaa cccagcgaca ggcaatgtgt cgcactcgaa actgtgggca aacgttgcct
     1021 gcgcggcggg gacggttaag tttgtgatgc tgcccgaccc gtcggcggag atttgggcgg
     1081 tgtatttggg cattgtcggc ggctatgcgg tggcgcgttc gttggtcagc gtcaaacgtc
     1141 aggaggtcga gaatgaatct cgtgaaactg ctggcgaata actggcaacc gattgccatc
     1201 atcgcgcttg tcggcacgct cttggcggtg tcgcaccatc aaggctacaa gtcggctttt
     1261 gcgaagcagc aggcggtcat tgacaagatg gaaaaagaca aagcgcaagc cctgctgttg
     1321 tcggctcaaa actacgcccg cgagctggaa caggcgcgtg cggaagctaa aaaatatgaa
     1381 gtcaaggcgc acgccgtcgg catggctttg gcgaaaaaac aggcggaagt cagccgtctg
     1441 aaaacggaaa ataaaaagga aatcgaaaat gtccttactc aagaccgtaa aaatgcaggc
     1501 ggcggttgta ttgacggctt tggccatcac ggcttgcagc tctacaagcg cgccctcggc
     1561 tacggaaatt aaggttgtcg aaaaggcggt catgccgaca ccgcctgccg cattgatggt
     1621 cgcgccggtg cgcccgaatc cgccgaaaga cggcaagacg gccacgctgt tggaacacgc
     1681 cgccgagttt ggcggctatg ttgccgaact ggaaaatcaa aatcaggctt ggcgcgactg
     1741 ggcgggcaat cactcccgca aagtcggaaa ctgacaaaaa agcccgcgta gggcgcgggc
     1801 tgagggtgaa agcggatttt atacctcttt tacaggggta gcggcggtag tgcttttcag
     1861 caaatcgact gcgtgctgac agttttgctt gctggtgtag ccttcgccct gagcgatgat
     1921 ttcatggttg gctgctttca aacgccaacg gtattcgcct tttgcgtctt tatagatttc
     1981 aaaatacata aggtttctcc tatgaatgag tacacgtttt cttaccgctt taacggcaag
     2041 tcctggtcat tgagcatttg ggcggacaac cctgaagaag ccagggcgaa atttcgggct
     2101 gcacgagaaa atgcgcacta tgacggcgaa gttgtagcaa aggtttatac atttgtaaat
     2161 atttcgtggg ttaagaaatt gtacaagcgg acaaaatatt taatgggtat caaagaatga
     2221 cctaccgtga attagttgaa cgtcagttgg ctgtgcgcca tgccgatttg gaattgggct
     2281 taagccgcgc acgcgaacaa gagccgtttg tcattcatgt ttccgatctg ttggataagg
     2341 caggcattga gtacgcggta cgcatggata aggattttca gacg
//
LOCUS       DAWEWP010000205         2374 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWEWP010000205 DAWEWP010000000
VERSION     DAWEWP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.205"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            42..1250
                     /locus_tag="WGN53_001929"
     CDS             42..1250
                     /locus_tag="WGN53_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0140863.1"
                     /translation="MSEAEYFSHFISNGNGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNHGDMEIPLDILINQGSYFSGAFPICQKFKNMPNPERFDYRKKV
                     ANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKGLEPEKYSLAGLK
                     ESLKFGFIHEQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLD
                     RMYDLHANQN"
     gene            1271..1582
                     /locus_tag="WGN53_001930"
     CDS             1271..1582
                     /locus_tag="WGN53_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140864.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            1586..1786
                     /locus_tag="WGN53_001931"
     CDS             1586..1786
                     /locus_tag="WGN53_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140865.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            2013..2243
                     /locus_tag="WGN53_001932"
     CDS             2013..2243
                     /locus_tag="WGN53_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140866.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDASAIGTQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVR"
BASE COUNT          758 a          397 c          554 g          665 t
ORIGIN      
        1 ggggagcgaa ataaaacaaa tccctaaagg tactgaacaa aatgagtgaa gcagaatatt
       61 tttcccattt catatctaac ggcaatggga agttattaga aattccgcaa cgtaggggca
      121 ggcaggacgg cgtttttata gattggttgt cattcacact gcacgaagat tccttgctga
      181 aagtttccgg atgcccctta gtttccgatg ccgaatatat gtttgtttta agcaaaaaat
      241 tggaggaaat attagggttt ggcatcacga gcagatgcaa atcgaagggc aataaatttt
      301 acgattcgat gtttaggttg ggatcggaag aagttgacta cggcgaagtc cattacggag
      361 gtcagcgaaa tacggtttta atcgaattga aaggtgtagg ttgcaacatt gcaaatccag
      421 gttgggaatt gaggcttaag cagtttttgg aagattcatt gaggccgagg ataacgcggg
      481 tagatttggc acttgatttt tttgatgggg agtacacgcc ggaacaggca cttttggatc
      541 acgataacgg ttttttcgat aacagtaaca tgaggccgaa atctgaaatg gttggaacgg
      601 cttggcggag agaggacggg agcggcaaga cattttatgt aggtcgcaag aaaaattctc
      661 gttttgtgcg tgtttatgag aaaggcaggc agctaggcga taaagaaagc aagtgggtaa
      721 ggttcgaaat tcagtttaat catggagata tggaaatacc tttggatatt ctgataaatc
      781 aaggttctta cttttcaggc gctttcccga tttgtcagaa atttaaaaat atgccgaatc
      841 cggaaaggtt cgattaccgt aaaaaagtgg ctaatttaac ttttcagcat aaattgagat
      901 acgcaaaaaa cgcggtcggc aaactgatta atttcatgtt tgatatgggt tttgatagtg
      961 atgaaattgt cagatatctg aaggcagatt tggggtatcc caaagggcta gaacctgaaa
     1021 aatattcgtt ggccggattg aaggaatctt tgaaattcgg ctttatccac gaacaaccgg
     1081 atgtagattt agaggttgaa ttggaagaac tcggaattat caaatttaag caatcagata
     1141 aattcgatcc ggataaaagg cttttcgatc cacatcacga tgtagaaagt gagaggcaat
     1201 atcagcttta tctcgacaga atgtatgatc ttcatgcaaa tcaaaattaa cctaaaaagg
     1261 aaaaattaat atgtttaatc aaactcaaac tgtaacttat cccgcaactt ttttaggagc
     1321 taaaaaattc aaaggcgaaa ttgatggctc taatatcgac acttgttccg tattggttgc
     1381 aacacctttg ccggcacagt cgggaaatgc tgttggattc acggcagcac aaatgaagtt
     1441 cggggacagt aagaatttct caaaattaga gaatctcaaa tacccgtgcg aagttatggt
     1501 aacggttgaa atgacttcga caggtaaggg catggttcct tcattaattg attttcaggt
     1561 ggcagaaaag ccgaaaggtt gatttatgaa atttgaagaa cgtttcatag ttcaagactt
     1621 ggaaacgcat gactttattt atcccgatcc tttcggtgat gtggggttta ctcaaaatat
     1681 taaatcagca ggtcaatttg aaagctacga agatgcgttg aattcaggca taaatgaaat
     1741 aggcggagga ttccagatat ttcagttctt cgtaaaatcg gaataaaaga aaaacaggct
     1801 cggcgggcgg tctgtcaacc tttcacaaag cccgcaaaaa ggattaaaaa tgaataaagc
     1861 aactttaatt atcggatttt ttgtttgcct cattcttttc attttctcaa ttctctattt
     1921 cataaattaa aaacaaaatt cgggagcagg cttcgcccgg atttaaacct aagaagccgt
     1981 attactttaa atatcaaaaa aggaaaaaaa cgatgaacat cgttaaaaaa tacgctgtaa
     2041 aagcagcctt ggcagccggt atcttcacac cggccattgt tatggcagat acctttgatg
     2101 catccgcgat tggtacgcaa gtagcgaatg taatcatggg tttcgtgtca atggtttccg
     2161 ccgtgggtat ggcggccatt accgtgattc ttgcaatcca aggcttcaaa atggcttgga
     2221 gcatgattaa atctgtcaga taaacagagt gaagaaaaag gggcgtataa atgggctatc
     2281 gtgtcggcat aaattgtttt gatacaagat tgcaggcaga cgactattta ttgtcgtccc
     2341 ttcctcctac tgttacccag gacggaaaaa tcat
//
LOCUS       DAWEWP010000206         2338 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWEWP010000206 DAWEWP010000000
VERSION     DAWEWP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.206"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            43..2256
                     /locus_tag="WGN53_001933"
     CDS             43..2256
                     /locus_tag="WGN53_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0140867.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNVPDNPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGS
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPHVLASLGDVKGVLSVTRL"
BASE COUNT          599 a          755 c          564 g          420 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac gaaagaccca tcatgaccgc catcagcccg
       61 attcaagaca cgcaaagcgc gactctgcaa gaattgcgcg aatggttcga cagctactgc
      121 gccgcgctgc cggacaacga taaaaaactt gtcttagccg cccgttcgct ggcggaagca
      181 cattaccccg ccgatgccgt cacgccgtat ggcgagccgt tgtccgacca cttcctcggc
      241 gcggcacaaa tggtcgacga actcgacctg ctgcccgatg ccgtcgccgc caccctgctt
      301 gccgacatcg gacgctacgt ccccgattgg aatctattgg tttccgaacg ctgcaacagc
      361 accgtcgccg agctggtcaa aggtgtggac gaagtgcaga aactcaccca cttcgcccgg
      421 gtggacagcc tcgccacgcc agaagaacgc gcccagcagg cagaaactat gcggaaaatg
      481 ctgctggcga tggttaccga catccgcgtc gtgttaatca aactggcgat gcgtacgcgc
      541 accctgcaat tcttaagcaa cgttcccgac aatcctgaaa agcgcgccgt tgctaaagaa
      601 accctcgaca tttttgcccc actcgccaac cgcttgggcg tgtggcaact caaatggcag
      661 ctcgaagatt tgggcttccg tcatcaagag ccggaaaaat accgcgaaat cgccctgctt
      721 ttggacgaaa aacgcaccga gcgcctcgaa tacatcgaaa acttcctcaa tatcctgcgt
      781 acggaactca aaaaatacaa tatccacttt gaagtcgccg gccgcccgaa acacatctac
      841 tccatttaca aaaaaatggt gaagaaaaaa ctcagcttcg acggcctctt tgacatccgc
      901 gccgtgcgga ttctggttga taccgtcccc gagtgttaca ccacgctggg cattgtccac
      961 agcctttggc agcctatccc cggcgagttc gacgactaca tcgccaaccc gaaaggcaac
     1021 ggctataaaa gtttgcacac cgtcatcgtc ggtcccgaag acaaaggtgt ggaagtgcaa
     1081 atccgcacct tcgatatgca ccaattcaac gaattcggtg tcgccgccca ctggcgttac
     1141 aaagaaggcg gcaaaggcga ttccgcctac gagcaaaaaa tcgcctggtt gcgccaactt
     1201 ttggactggc gcgaaaatat ggcggaaagc ggcaaggaag acctcgccgc cgccttcaaa
     1261 accgagcttt tcaacgacac gatttatgtc ctgaccccgc acggcaaagt cctctccctg
     1321 cccacgggcg cgacccccat cgacttcgcc tacgccctgc acagcagcat cggcgaccgt
     1381 tgccgcggtt cgaaagtcga agggcagatt gtgccgctgt ccaccccgct cgaaaacgga
     1441 cagcgcgtcg aaatcattac cgccaaagaa gggcatcctt ccgtcaactg gctttacgaa
     1501 ggctgggtca aatccaacaa ggcaatcggc aaaatccgcg cctacatccg ccagcaaaac
     1561 gccgacaccg tgcgcgaaga aggccgcgtc caactcgaca aacagcttgc caaactcacg
     1621 cccaaaccca acctgcaaga gcttgccgaa aatctcggct acaaaaagcc agaagacctc
     1681 tacaccgccg tcggacaagg cgaaatttcc aaccgcgcca tccaaaaagc ctgcggcacg
     1741 ctgaacgaac cgccgcccgt acccgtcagc gaaaccacca tcgtcaaaca gtccaaaatc
     1801 aaaaaaggcg gcaaaaacgg cgtgctcatc gacggcgaag acggtctgat gaccacgctt
     1861 gccaaatgct gcaaacccgc gccgcccgac gacattgtcg gcttcgttac ccgcgatcgc
     1921 ggcatttcgg tacaccgcaa aacctgcccc tctttccgac acctcgccga acacgcgccc
     1981 gaaaaagtac tggacgcaag ttgggcggcg ttgcaggaag gacaagtgtt cgccgtcgat
     2041 atcgaaatcc gcgcccaaga ccgctccggg cttttgcgcg acgtatccga cgcgctcgcc
     2101 cgccacaaac tcaacgttac cgccgtgcaa acccagtccc gcgacttgga agccagtatg
     2161 cggttcacgc tcgaagtcaa acaggtcaac gacctcccgc acgtcctcgc cagcctcggc
     2221 gacgtcaaag gcgtattgag cgttacccgg ctttaaatac aaaaatgccg tctgaaagcc
     2281 gtataacgct tcagacggca ttttgattga cggggtttgc tattttttgt tgcatagt
//
LOCUS       DAWEWP010000207         2336 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWEWP010000207 DAWEWP010000000
VERSION     DAWEWP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.207"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            30..1247
                     /locus_tag="WGN53_001934"
     CDS             30..1247
                     /locus_tag="WGN53_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0140868.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTAIAIIGLANIAGCVF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLKMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            complement(1450..1590)
                     /locus_tag="WGN53_001935"
     CDS             complement(1450..1590)
                     /locus_tag="WGN53_001935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140869.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1603..>2336
                     /locus_tag="WGN53_001936"
     CDS             1603..>2336
                     /locus_tag="WGN53_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0140870.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSR"
BASE COUNT          547 a          724 c          540 g          525 t
ORIGIN      
        1 gcaaacttaa ccgactcaaa atacacatta tgacgcacac cgcatcgaaa acacccaaat
       61 tttggctgac catcaccgcc gccgcattca tcctgctgat taccatcggc atgaggatga
      121 cgctcggact gttcgtccaa cccgtcgtca acaccaccga attgagcatt gcccaattca
      181 gcctcatcat cgccgttttc caactgatgt ggggcatatc gcaaccattg tccggcgcgc
      241 ttgccgaccg tttcggcgcg ttcagggtat taagcggcgg cgccgtcctg ctcgtctgcg
      301 cctgcctgat tgcccccaac atcccgactt attgggggct gatgattgcc gtcgggctgc
      361 tgctcgcatt cggcaccggt tccggcggtt tctccatcat tatggggcag gtcgccgccc
      421 aagtccccgc acacaaacgc ggcttggcat ccggactggt caacgccggc ggttcggcag
      481 gacaattcct gttcgcaccg ctggttcaag gactcgtcgt cctgcccgaa gtcggctgga
      541 cgggcacatt ttacgtttgg ggcgcaatcg ccctgctgat tctgcctgtc tcatggtggc
      601 ttgccggcgg caacaacgcc gcccacaccc aacacgccca agccacacac ggacaaagcc
      661 tcggagaagc agtcaaaacc gccttcaaaa ccccaagcta catcctgctg cacctaagct
      721 tttttgcctg cggcttccac atcgcctttc tcgtaaccca cctacccacg gaagtcgccc
      781 tgtgcggact gcccgccacc gtcgcatcca cagccatcgc catcatcgga ctggcaaaca
      841 tcgccggttg tgtgttctca ggctggtgca caagccgcgt tcggggcaaa tacgtcctgt
      901 tcggactgta cgcctcgcgc gccgcgatga tactcatcta cattttttcg cccaagaccg
      961 acctcaactt ctacattttc gccgccgcac tcggatttac ctggctcgcc accgtcacgc
     1021 cgaccgcctc cattaccggc aaactcttcg gcacacgcta cctcgccacc ctgttcggac
     1081 tgaagatgct cagccaccaa atcggcggat tcctcggctc gtacatcggc ggcatcgtga
     1141 ttacacaatt cggcgactac ggctggatgt ggtacgccga cgcactgctg gcgggaaccg
     1201 ccgccctcct caacctcccc atccgcgaac cccgaaccgc cgcatgaaag aatgccgtct
     1261 gaaaaccctc agacggcatt ttccaaacac cccgcccgtt tccggactgc acgacacgca
     1321 aatcagcctt atcaaaccgc aaacaaccgc cgcgattaaa ccgccattca aataagtccg
     1381 ttttcccttc gggtgtaaaa tcatcacaaa acgcgatact gtatatcgcc gggcgttatg
     1441 cagcggattt caggataaaa tgccgtctga agccgcccct gcccgggcgg gcgaagcgtc
     1501 ggatgcggtg cgcttggcaa aggtttgccc gccgcgcccc gtatccggca tttcatcaat
     1561 gcagaccgca tttccaatcc tataattcaa ggtgtccgcc ttatgaaaat cgtttttatc
     1621 acaacagtcg catccagcat ttacggtttc cgcgcccccg tcattaaaaa attaatcggc
     1681 aaaaaccatc aggtgtatgc ctttgtatcg gagttttccg ataatgaatt ggatattatc
     1741 agggaaatgg gggttacacc cgttacctac cgttcaaacc gcagcgggct gaacccgttt
     1801 tccgatataa aatccacctt cctcatattt aaagaactca aaaaaatatc gccggatttg
     1861 gttttccctt atttcgcaaa acccgtgatt ttcggcactt ttgccgcaaa actggcaggc
     1921 gtgcccagaa tcgtcgggat gctggaaggt ttgggattcg catttacccc gcaaccggaa
     1981 ggcataccgt taaaaacaaa aatcataaag gggattttga ttgccttgta ccgcattgcc
     2041 ctgccgatgt tggaaagcct gattgtatta aaccccgacg acaaagacga actgacggac
     2101 aaatacggca tcaaaataaa aaacatccat attttgggcg gaatcggtct ggatttgcgg
     2161 caatatcctt attccgaggc ggatattccc gatgaaaaag aacccgtaaa attcctcttt
     2221 atcggcagat ttctgaaaga aaaggggatt gatgatttta ttcgggcggc ggaacaggtt
     2281 aaggacaaat accccgatac ggtttttacc gctttgggcg caatcgacaa atcacg
//
LOCUS       DAWEWP010000208         2330 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWEWP010000208 DAWEWP010000000
VERSION     DAWEWP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.208"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..410)
                     /locus_tag="WGN53_001937"
     CDS             complement(<1..410)
                     /locus_tag="WGN53_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0140871.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAVLFY
                     RKIRTVINHHLALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNRRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSS"
     gene            1016..1831
                     /locus_tag="WGN53_001938"
     CDS             1016..1831
                     /locus_tag="WGN53_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0140872.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNNRINQTEED
                     SYSTSVGSCINGFTVQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSNKK"
     gene            1848..>2330
                     /locus_tag="WGN53_001939"
     CDS             1848..>2330
                     /locus_tag="WGN53_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140873.1"
                     /translation="MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADNAA
                     AIVGKDTNLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINKNNGR
                     LPNTSEIERSYYKAVTYHGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     V"
BASE COUNT          746 a          420 c          482 g          682 t
ORIGIN      
        1 tagctgctca gactatcggt ataaacaata ctatccggca tgattttctt tttgatgaca
       61 gggagtaacg tttcagactt ggcattatcc accacaacgg tatagacccg tctgttgcgt
      121 ttcagaatgc cgaaaacaac cacttttcct gctgcaccgc gaccacgtct gcctttacgc
      181 cgtccgccga aatcgctttc gtccggctcg acagggccct caaaaacctc atcggcagcc
      241 aaggccaaat gatggttgat aaccgtgcgg attttacggt agaacagtac tgccgaattg
      301 ggatggatac ccaaaatatc ggcggcagaa cgggcggtaa cttccagcac aaaaaaacgg
      361 agcagttctt tctgtacttt tttctttaat ttgcagtgtg ttatcttcat atttcgaggg
      421 taacatatct gctaatctag tacagcccca aaatatacca aaaacagcaa aacaaattgt
      481 aaggatacgt ataggctttg taaaggtaga ttgtgaaaaa agcagttttt taaacgaatg
      541 aaacggcttc gggctgaaat atatgctgat gccctgtcct tcccgtatat cttgtgtgtt
      601 gtcaaagtgc aggctgcttt gaaattggca ttacagtcga tgaaccacca ttttgcttta
      661 tttcagcggg cttgagatgt gtataagaat attgttttga ataaatttaa agaaaatgat
      721 aatcgttatt gacgattttt aaaggaaagc gtagagtgcc aattctatga agcaatacgg
      781 taagtaacaa tgaaaatatc tactgcttgg gtatagagca tatttcacaa cccgtaacta
      841 ttcttgcgga aacagagaaa aaagtttctc ttctatcttg gataaatata tttaccctca
      901 gtttagttaa gtattggaat ttatacctaa gtagtaaaag ttagtaaatt atttttaact
      961 aaagagttag tatctaccat aatatattct ttaactaatt tctaggcttg aaattatgag
     1021 accatatgct actaccattt atcaactttt tattttgttt attgggagtg tttttactat
     1081 gacctcatgt gaacctgtga atgaaaagac agatcaaaaa gcagtaagtg cgcaacaggc
     1141 taaagaacaa accagtttca acaatcccga gccaatgaca ggatttgaac atacggttac
     1201 atttgatttt cagggcacca aaatggttat cccctatggc tatcttgcac ggtatacgca
     1261 agacaatgcc acaaaatggc tttctgacac gcccgggcag gatgcttact ccattaattt
     1321 gatagagatt agcgtctatt acaaaaaaac cgaccaaggc tgggttcttg agccatacaa
     1381 ccagcaaaac aaagcacact ttatccaatt tctacgcgat ggtttggata gcgtggacga
     1441 tattgttatc cgaaaagatg cgtgtagttt aagtacgact atgggagaaa gattgcttac
     1501 ttacggggtt aaaaaaatgc catctgccta tcctgaatat gaagcttatg aagataaaag
     1561 acatattcct gaaaatccat attttcatga attttactat attaaaaaag gagaaaatcc
     1621 ggcgattatt actcatcgga ataatcgaat aaaccaaact gaagaagata gttatagcac
     1681 tagcgtaggt tcctgtatta acggtttcac ggtacagtat tacccgttta ttcgggaaaa
     1741 gcagcagctc acacagcagg agttggtagg ttatcaccaa caagtagagc aattggtaca
     1801 gagttttgta aacaattcaa ataaaaaata atttaaagga tcttattatg aatgagggtg
     1861 aagttgtttt aacaccagaa caaatccaaa ccttacgtgg ttatgcttcc cgtggcgata
     1921 cctatggcgg ttggcgttat ttggctaatt tgggcgaccg ttatgcggat aatgctgctg
     1981 caattgtcgg taaggataca aacttaaatg gtttgaattt atggatgaaa aaaggggtgg
     2041 aaaacctatg ggatgatacg gtcggtaaaa agacccgttt agagaaattt gatcgggttg
     2101 cattgcaaca tttcagccaa tatgtagatc taattaataa aaataatggt agattaccta
     2161 acactagtga aattgagaga agttactata aagccgttac ctatcatggc gtttcttcta
     2221 gtgcagctat tgatttagtt attaatcgtt cacttccgga tatggcggat ggttattggg
     2281 cattaggttt ggggatagaa gccgaacgta tccacaatga gcaagcagta
//
LOCUS       DAWEWP010000209         2248 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWEWP010000209 DAWEWP010000000
VERSION     DAWEWP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.209"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            295..807
                     /locus_tag="WGN53_001940"
     CDS             295..807
                     /locus_tag="WGN53_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ0140874.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPEILLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            887..1966
                     /gene="aroB"
                     /locus_tag="WGN53_001941"
     CDS             887..1966
                     /gene="aroB"
                     /locus_tag="WGN53_001941"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ0140875.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          555 a          700 c          543 g          450 t
ORIGIN      
        1 ttgataaaca tattatcttc aatatattca attggataat tgtttaccta agcaaagata
       61 attgcctttt cctgacaaat aagtgaaatc aacggattgt caaaacacag cctgaaataa
      121 aaaacctccc tgatttcttt tatttgtcct taaaatcaga aaggttcggg atggtcgggt
      181 tatttttcca aacgtaccgc cgccctgccg atttcgtata aaattccgcc gtaacccgac
      241 aagcccgaac cctgtcgccc cgaaaggcgg ggtgtcaaac attaaggaat tgtgatgaaa
      301 aactttaacg gcaaactcat cctcatcgga ctgatgggcg cgggcaaaac cacgctgggc
      361 cggcaaatgg cgcagcggct ggattaccgt ttttacgaca gcgatcacga aatcgccgca
      421 gccgccggcg ttcccatccc caccatattt gaaatggaag gcgaacaggg attccgttcg
      481 cgcgaaaccg ccatactcaa aaagctggtt atcctgcccc atatcgtcct gtccaccggc
      541 ggcggcgcgg tgttaaaaga agaaaaccgc gcccttatcc gcaaaagcgg cacggtcgtc
      601 tatctgcacg ccccgcccga aatcctgctc gaacgcacgc gctgcgacaa cagccgtcct
      661 ttgctgcaag ttgccgatcc tttggcgaaa ttacgtgaac tctacgccgc acgcgacccc
      721 gtttaccgcc aaaccgccga ctttaccgta gaatcggcaa actgccggga aaccgtgcaa
      781 accctgctca aacgcttatc ccgataaacc ggcatatgcg ccgcgcccag aaaaccaaac
      841 cgcgccccgc ccggcgggcc ggcggttcaa actttaagga acaacaatga aaacactgac
      901 cgtacacacc ccgtcccaca gctaccccat ctttatcggc aacggactgc tgccgcaggc
      961 aggaagcctg ctcaaaccgc atttgggcaa acgcgccgcc atcatcgcca acgaaaccgt
     1021 cgccccgctc tacctcggca cgcttcagac ggcattggat gcggcaggcg tatcccattt
     1081 cagcatcatc ctgcccgacg gcgaggcgca caaaaactgg cagacgctca acctcatctt
     1141 tgacgggctg atgcaaaacc gcgccgaacg caaaaccaca ttaatcgcac tgggcggcgg
     1201 cgtgatcggc gacatggtcg gctttgccgc tgccacctac cagcgcggcg caccgttcgt
     1261 tcaaataccg accacactgt tgagtcaggt cgactcatcg gtgggcggca aaaccgccat
     1321 caaccacccg ctcggcaaaa atatgattgg cgcgttttac cagccgcagg cggtgcttgc
     1381 cgacttggac acgctgcaca ccctgcccgc ccgcgaattg tccgccggta tggcggaagt
     1441 catcaaatac ggcgcgctcg gcgacatcgg cttttttgaa tggctggaac agcatatgcc
     1501 cgaactgatg acgctcgatc gggaaaaact cgcccaagcc gtgtaccgct gctgccaaat
     1561 gaaggcagac atcgtcgccc aagacgaaac cgaacagggc atacgcgcat ggctcaacct
     1621 cggacacacc ttcggacacg ccattgaaac cgagatgggt tacggcactt ggctgcatgg
     1681 agaagccatc gccgccggct gcgtgttggc ggcgcgtttg tccgaacaac tgggcaaaac
     1741 ctccgccgca gataccgcgc ggctcgccgc cctgctcgaa gccgccggac tgccgtccgc
     1801 gccacccgtg tttgcctttg aaaaatggct ggaacacatg agccacgata aaaaagtcag
     1861 cggcggcatc atgcgcttta tcggtctgaa ccggctgggc gaagccaaca tcaccgaaat
     1921 taccgacacg gacatcctcc gccgcaccct gcaaccgtat ctctgatttc ctctgccgat
     1981 gtgctgccgc gcgggtttga cgcacgatga tgattttcca tcatctttct ccgcaaaagc
     2041 gggaatccag tccgttcggt ttcggtcgtt tccgataagt tcccgttgct tttcatttct
     2101 agattcccac tttcgcagga atgacggtgg aaaggttttt gttgtttcgg agaagtttct
     2161 gcaaccctag aatctcgtta tttccacaaa aaacagaaac ccgaaacagc aacttaaaac
     2221 ctcgtcattc ccgcaaaagc gggaatcc
//
LOCUS       DAWEWP010000210         2238 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWEWP010000210 DAWEWP010000000
VERSION     DAWEWP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.210"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..953
                     /locus_tag="WGN53_001942"
     CDS             <1..953
                     /locus_tag="WGN53_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0140876.1"
                     /translation="SGGRFYERCWLMGSEDVLYGRVHFGGQNNTILFELTGTGCGVAK
                     EGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRNKGMFTCHHVKPKGEC
                     LGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGDKTSTWTRFEIEFKAKDI
                     VIPFEVLQNPGEYFGGAYPICERFAQKATRIHAVKEDKVISADRYLEWVKKQFGRAAN
                     GLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKAQAIHEQPAFKPYKDPYY
                     MYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
     gene            998..1288
                     /locus_tag="WGN53_001943"
     CDS             998..1288
                     /locus_tag="WGN53_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140877.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1293..1490
                     /locus_tag="WGN53_001944"
     CDS             1293..1490
                     /locus_tag="WGN53_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140878.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            1498..1782
                     /locus_tag="WGN53_001945"
     CDS             1498..1782
                     /locus_tag="WGN53_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140879.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            1789..2067
                     /locus_tag="WGN53_001946"
     CDS             1789..2067
                     /locus_tag="WGN53_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140880.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSLKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
BASE COUNT          673 a          519 c          511 g          535 t
ORIGIN      
        1 gctcgggcgg tcgtttctat gagcgctgtt ggttgatggg ttcggaagac gtcctatacg
       61 gtcgcgtcca ttttggcgga caaaacaata ccatcctttt cgaactgacc ggcaccggtt
      121 gcggcgtcgc aaaagaaggc tgggaatcac gacttttcgc attcctgact aatgcaatcc
      181 gcccaaaaat cacacgcgtt gacatcgcaa aagacttttt caacggcgaa tacagcccga
      241 accaagcccg tgaagaccga aataaaggta tgtttacctg tcatcacgtc aaaccaaaag
      301 gcgaatgttt ggggtcagat tgggaagaag acgatgaagc caaaatgacc aaaggcaaga
      361 cctatggtat cggctcccgt gaatcgtcca aatatgtccg cgtctatgaa aaaggcaagc
      421 agttgggcga taaaacaagc acatggacgc gctttgaaat tgaattcaaa gcaaaagaca
      481 tcgttatccc tttcgaagtt ttgcagaatc cgggcgaata tttcggcggc gcatatccga
      541 tttgcgaacg attcgcccaa aaggcaacgc gcatacacgc ggttaaggaa gataaggtca
      601 tttcagccga ccgctacctt gaatgggtaa aaaaacagtt cggacgtgcg gcaaacggtc
      661 tgaaattcat ttttcccgaa ttggacaaag ccaaactgtt tgaactgatt gagccgagtc
      721 atcacaagct gcccaagtct ttggctcccg aagcctacga ctgcgccttt ttgaaagctc
      781 aagccattca tgaacagccc gcattcaaac cgtacaaaga cccttactat atgtacgaat
      841 attacgagaa tcttgaaaaa cagcttgaac agcaaaaaca cgtcaacaat gaagaaagct
      901 ataacaactt catttacgac aaattcgcaa gactaccgat ttcatgggct taaagtgtct
      961 gcccgaaaga cgtttaatca cacaaggaaa ccaaaaaatg aacatccaac ttcaaggcca
     1021 catcgtcggc gttaaaaaat tcaacggaca aatcgaaggc aagagcttcg actattgccg
     1081 cctgattgtc gccacaccct tagacagctc ccaaggcaac gcattgggca gctctactac
     1141 tgaatacgat ttcggcggct ctgccaattt cgagcagttc cgaaacgccc aatttcccat
     1201 cgaagcaaac ctgaacgtgg aaatcgttac tacgggcaaa acccaaaaac tgaaagtcat
     1261 cggttttcaa ctcgtgaaga aaggctgatt gaatgcagaa agtctatgtt gtccagtccg
     1321 tatcaacagg ggactttctg tatctctctc ctgaaacggg cgacatcgga cataccaaat
     1381 taatcaccaa tgccgattat ttctacgact tcgaagaagc gattaacgca ggtttggaag
     1441 aaatcggcaa ccaatacgaa tttgtcgtat tcggattttt gaaagactga ttttcggatg
     1501 ttcggcggtc gtctgaaaaa cgctccatcc attaccgcca aacacttttt gaaggaaaat
     1561 atcatgaaat ttattaacac ctgccgtaaa tacggcgcaa aactggctgt tgtaacagct
     1621 gctcccctgg ctttggccgc acatgcaaat gcaacgttgc ccgatacggc aaaaaacgct
     1681 ttggaagccg caaaagcgga cggtatggaa gccggttgga ttgtcgttgg cgttttcgcc
     1741 gcgctttttg tattttccat cgttaagaga gtgatgaagt aagacggcat gtactaccaa
     1801 gtcggaaata aatgtcttga gaagcaccag gctgaaaacc tttatttcag cttggtagta
     1861 ccaagaatca aagaaaacgg acagattgtc aggccggaat ataacggcag cctgtggaag
     1921 atgtcggacg gtcagccgct aaggctttta ttggcggaat gcagtctgaa agacaacctg
     1981 caaagcggcc ttgaaacagg ctggatagta ttcggcatcc tcgcgtccgt ttactttgtt
     2041 tccctgctga aaaaggtttt gaaatgatgg atttttattt ttatctcggc gtttccgtac
     2101 ccgtattaat cggggcggtt ctgtttaaga attgagcgca tgaagttatg gtgtcaaaat
     2161 caggctttca aaacaatcat tgaaaggcag aaccatgaac aagccgttta tcactcaggc
     2221 gcagttggca ctttataa
//
LOCUS       DAWEWP010000211         2169 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWEWP010000211 DAWEWP010000000
VERSION     DAWEWP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.211"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            99..1910
                     /gene="typA"
                     /locus_tag="WGN53_001947"
     CDS             99..1910
                     /gene="typA"
                     /locus_tag="WGN53_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ0140881.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKHTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          543 a          630 c          569 g          427 t
ORIGIN      
        1 gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc gtggtataat
       61 gcgcagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg
      121 ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg ctgcgccaat
      181 ccggcacatt ccgcgccaac cagcaggttg acgagcgcgt gatggacagc aacgacctcg
      241 aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac gaaggctacc
      301 acatcaatat cgtcgatacg ccgggacacg ccgacttcgg cggcgaagta gagcgcgttt
      361 tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa
      421 cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc gtcatcaata
      481 aaatcgacaa gccgtctgca cgtccgagct gggttatcga ccaaactttc gagctgttcg
      541 acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttacgct tcaggtttga
      601 gcggctttgc caagctggaa gaaaccgacg agagcagcga tatgcgcccg ctgttcgaca
      661 ccatcttaaa acacacgccc gcaccgagcg gcagcgcgga cgagccgctg caactgcaaa
      721 tttcccaact cgactacgac aactacaccg gccgcctcgg catcggtcgt atcttgaacg
      781 gacgcatcaa acccggccaa accgttgccg tcatgaacca cgagcagcaa atcgcccaag
      841 gccgcatcaa ccagcttttg ggtttcaaag gcttggaacg cgtaccgctt gaagaagccg
      901 aagccggcga catcgtgatt atttccggta tcgaagacat cggcatcggc gtaaccatca
      961 ccgacaaaga caaccccaaa ggcctgccga tgttgagcgt ggacgaaccg acgctgacga
     1021 tggactttat ggtcaacacc agcccgctgg cgggtacgga aggcaaattc gtaaccagcc
     1081 gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag
     1141 acaccgccga tgccgacgtg ttccgcgtat ccggacgcgg cgagctgcac ctgaccattt
     1201 tgttggaaaa tatgcgccgc gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt
     1261 accgcgacat cgacggtcaa aaatgcgaac cttatgaaaa cctgaccgtg gacgtacccg
     1321 acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa ctgactaata
     1381 tggaaagcga cggcaacgga cgcacccgcc tcgaatacca tattccagcg cgcggcttga
     1441 tcggtttcca aggcgaattt atgaccctga cgcgcggggt cgggctgatg agccacgtgt
     1501 tcgacgatta cgcgcccgtc aaacccgata tgcccggccg ccacaacggc gtgctggtgt
     1561 cccaagagca gggcgaggca gtcgcttacg ccttgtggaa tctggaagac cgcggccgta
     1621 tgttcgtatc gcccaacgac aaaatctacg aaggtatgat tatcggcatc cacagtcgcg
     1681 acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc cgtgccagcg
     1741 gtaccgacga agccgttcgc ctgaccacgc cgattaagct gacgctggaa ggtgcggtcg
     1801 agtttatcga cgatgatgag ctggtagaaa tcacgccgca atccatccgc ctgcgcaagc
     1861 gttacctgag cgaattggaa cgccgccgcc atttcaaaaa gctagattga tgtttactgg
     1921 atttaatgtt taaatgatac tcgatgccgt ctgaaaaagt tcagacggca ttttttattc
     1981 ggacgggctt tgcggcttct tgaagctgtt tcagacggcg tttttcctac ccaatcaaga
     2041 aactgccgcc atttttccag cggcatatcg ccccgtcgcg tttcggtatc gggttcggct
     2101 tcccggcagc atgtcgaaca atgccgtttg aaggaatacg ccttttgagt ccttacaagc
     2161 ttaggaaga
//
LOCUS       DAWEWP010000212         2125 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWEWP010000212 DAWEWP010000000
VERSION     DAWEWP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.212"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            123..1508
                     /locus_tag="WGN53_001948"
     CDS             123..1508
                     /locus_tag="WGN53_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ0140882.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFSIV"
     gene            1528..2046
                     /locus_tag="WGN53_001949"
     CDS             1528..2046
                     /locus_tag="WGN53_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ0140883.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          446 a          645 c          572 g          462 t
ORIGIN      
        1 gttcaaataa acaggaatat aacttcagac aaacaactta ccgcccgatt tgtgctatcg
       61 ttttcgcaca acttaaaaaa cctgacaatt ttgtactttt attacagaga aaggctttac
      121 aaatggacgg ctggacacag acgctgtccg cgcaaaccct gttgggcatt tcggcggcgg
      181 caatcatcct cattctgatt ttaatcgtca aattccgcat ccacgcgctg ctgacactgg
      241 tcatcgtcag cctgctgacg gctttggcaa ccggtttgcc cacaggcagc attgtcaacg
      301 acgtactggt caaaaacttc ggcggcacgc tcggcggcgt ggcgcttctg gtcggcctgg
      361 gcgcaatgct cggacgtttg gtcgaaacat ccggcggcgc acagtcgctg gcggacgcgc
      421 tgatccggat gttcggcgaa aaacgcgcac cgttcgcgct gggcgttgcc tcgctgattt
      481 tcggcttccc gattttcttc gatgccggac taatcgtcat gctgcccatc gtgttcgcca
      541 ccgcacggcg catgaaacag gacgtactgc ccttcgcgct tgcctccatc ggcgcatttt
      601 ccgtcatgca cgtcttcctg ccgccccatc cgggcccgat tgccgcttcc gaattttacg
      661 gcgcgaacat cggccaagtt ttgattttgg gtctgccgac cgccttcatc acatggtatt
      721 tcagcggcta tatgctcggc aaagtgttgg ggcgcaccat ccatgttccc gttcccgaac
      781 tgctcagcgg cggcacgcaa gacaacgacc tgccgaaaga acctgccaaa gcaggaacgg
      841 tcgtcgccat catgctgatt cccatgctgc tgattttcct gaataccggc gtatcggccc
      901 tcatcagcga aaaactcgta agtgcggacg aaacctgggt tcagacggca aaaataatcg
      961 gttcgacacc gatcgccctt ctgatttccg tattggtcgc actgtttgtc ttgggacgca
     1021 aacgcggcga aagcggcagc gcgttggaaa aaaccgtgga cggcgcactc gcccccgtct
     1081 gttccgtgat tctgattacc ggcgcgggcg gtatgttcgg cggcgttttg cgcgcttccg
     1141 gcatcggcaa ggcactcgcc gacagcatgg cggatttggg cattcccgtc cttttgggct
     1201 gtttccttgt cgccttggca ctgcgtatcg cgcaaggttc ggcaaccgtc gccctgacca
     1261 ccgctgccgc gctgatggct cctgccgttg ccgccgccgg ctttaccgac tggcagctcg
     1321 cctgtatcgt attggcaacg gcggcaggtt cggtcggttg cagccacttc aacgactccg
     1381 gcttctggct ggtcggccgc ctcttggaca tggacgtacc gaccacgctg aaaacctgga
     1441 cggtcaacca aaccctcatc gcactcatcg gctttgcctt gtccgcactg ctgttctcca
     1501 tcgtctgaca gacggaaagg atagtaaatg actacgcatt ttgtcgttat gggcgtatgc
     1561 ggctgcggca agaccaccgc cgcgctgtcc ctgcagaaac acctcggtca atgtccctat
     1621 gccgaaggcg acgagttcca cacccaagcc aaccgcgaca agatgggcgc gggtattccg
     1681 ctgaccgatg aagaccgcta tccgtggttg ggcaatctgc gcgactggat gacgcaacag
     1741 gcgcaaaacg gtgcgaacca caccatcgta acctgttccg ccctcaaacg cggctaccgc
     1801 gacattctgc gcggagccga aggcaaagct gccttcatcc acctcagtcc gccgcaagac
     1861 atcaacctcg agcgcatgat gtcgcgcaaa ggacattata tgaaagcagg gatgctcgat
     1921 tcgcaactgg aaatcctcga ggaactgggc gaaggcgaat acggggtcaa aatcgccaac
     1981 cccggcacgc ccgaagcggt cgaagccgat attctgaact gggttgcctc ggaaaacctg
     2041 ctttgaagca atatgccgtc tgaagcccga cacaggatgg gtttcagacg gcataaacat
     2101 cgggaacaga atggattaca ttgat
//
LOCUS       DAWEWP010000213         1951 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWEWP010000213 DAWEWP010000000
VERSION     DAWEWP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.213"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(498..1580)
                     /locus_tag="WGN53_001950"
     CDS             complement(498..1580)
                     /locus_tag="WGN53_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0140884.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVEVSRVKDAGTYKA
                     QGGKSKTATQIADFGSKIGFKGQEDLGNGMKAIWQLEQKASIAGTNSGWGNRQSFIGL
                     KGGFGTVRAGNLNTVLKDSGDNVNAWESGSNTEDVLGLGTIGRVESREISVRYDSPVF
                     AGFSGSVQYVPRDNANDVDKYKHTKSSRESYHAGLKYENAGFFGQYAGSFAKYADLNT
                     DAERVAVNTANAHPVKDYQVHRVVAGYDANDLYVSVAGQYEAAKNNEVGSTKGKKHEQ
                     TQVAATAAYRFGNVTPRVSYAHGFKAKVNGVKDANYQYDQVIVGADYDFSKRTSALVS
                     AGWLKQGKGAGKVEQTASMVGLRHKF"
BASE COUNT          464 a          431 c          498 g          558 t
ORIGIN      
        1 ttagcttgtg ggtatttacc ggaaaaaaca gaaaccgctc cgccgtcatt cccgcgcagg
       61 cgggaatcta ggtctgtcgg cacggaaact tatcaggtaa aacggtttct tgagattttg
      121 cgtcctggat tcccactttc gtgggaatga cgggatgcag gttcgtggga atgacggaat
      181 gtaggttcat gggaatgacg cggtgcaggt ttccgtacgg atgaatttgt cattcccgcg
      241 caggcgggaa tctaggtctg tcggtgcgga aacttatcgg gtaaaacggt ttcttgagat
      301 tttgcgttct agattcccac tttcgtggga atgacggaat gtaggttcgt gggaatgacg
      361 gcggagaagt ttctgctttc ccgataaatg cccactctcg tgggaatgaa ggctgccgca
      421 ttaaatagca aacgaatcat ttcaaataaa aaagcctgtc tgacgacagg ctttttgttg
      481 ataccaatct tttcagatta gaatttgtga cgcagaccaa ccatgctggc agtttgttcg
      541 acttttcccg cgcctttacc ttgtttcaac caaccggcag aaaccagagc ggaagtgcgt
      601 ttggagaagt cgtagtcggc accaacgata acttggtcgt attggtaatt tgcgtctttc
      661 acgccattca ctttagcttt gaagccgtgg gcgtaagaaa cgcgaggcgt tacgttgcca
      721 aaacggtaag cggcagtagc ggcaacttga gtttgctcgt gttttttacc cttggtagaa
      781 ccaacctcgt tgtttttagc agcttcatac tgaccggcaa cagaaacgta caggtcattg
      841 gcatcgtaac cggcaactac gcggtgtact tggtaatcct taacaggatg ggcatttgca
      901 gtatttactg caacacgttc tgcatcagtg ttcaaatcag catatttggc aaaagaacct
      961 gcgtattgac cgaagaaacc ggcattttcg tatttcagac cggcgtggta agactcacgg
     1021 ctggacttcg tatgtttgta tttatccaca tcattcgcat tatcgcgcgg aacgtattgt
     1081 acgctgccgc tgaagcctgc aaatacggga gagtcgtagc gtacggagat ttcacggctt
     1141 tctacacgac cgatagtacc cagtcccagt acatcttcgg tgttagaacc agattcccat
     1201 gcattgacgt tgtcgccgct gtctttcaat acggtgttca gattaccggc gcggacggta
     1261 ccgaagccgc ctttcaagcc gatgaaggac tggcggttac cccagccgct gttagtgccg
     1321 gcgatggagg ctttttgttc caactgccaa atggctttca tgccgttgcc gaggtcttct
     1381 tgacctttga aaccgatttt agaaccgaag tcggcaattt gggttgcagt tttagatttt
     1441 ccgccttgag ctttatatgt accagcatct tttacgcgag aaacttctac gcctgcttta
     1501 attgtaccgt acaaggtaac gtcagccatt gctgcaacag gcagggcagc cagtgtcagg
     1561 gcaatcaggg attttttcat tgctgtattc cttttcatag ttaagaaatt taagccagcc
     1621 gggctttcca agccgcttag ctttgcattt accgccgacg ttttcggcga ttccatgtct
     1681 gaaactatag atgttcctgt ctgaaaaaac aaacttctgc ctgtttttgt gtggaattcg
     1741 atgtgtattt tgaagggcgg atagagatat tgtgggtatt ttttgtttta taacatatgg
     1801 ttatttaaat tttttaagat ttgcattttt acaacactta ctcgggaggg tattggaggg
     1861 cattgcaaac cgggggttat aaagacggca aaaaacgcgc acgtggtttc tttcggaacg
     1921 ggcggatgca aaacccgacc ctgcggcagg a
//
LOCUS       DAWEWP010000214         1947 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWEWP010000214 DAWEWP010000000
VERSION     DAWEWP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.214"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            247..1704
                     /locus_tag="WGN53_001951"
     CDS             247..1704
                     /locus_tag="WGN53_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0140885.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPIVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFIPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKCYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          439 a          579 c          443 g          486 t
ORIGIN      
        1 aaaccgccct atcggataag cccttaacag aaaaggcttt acccgcgccg tatcggaaca
       61 catcctctaa aatacaatcc gttgaattga aaaaaatata aaaacatccg cccgcgaaaa
      121 acggcagcgc gtcgtttgac aaagaatgaa aatatcggtt aaaaaccgat tttcatacaa
      181 aaaacaccgc tgccgtccgc atccgtttca gacggtattg agagaaaatc ttttaggaga
      241 acctttatgt cccggcatcc cgcccccacc ggagaaaaaa cattcttcgg ccaccccttc
      301 cagctttcca ccctcttcca tatcgaattg tgggaacgtt tttcatttta cggaatgcag
      361 ggcatcctgc tgatttacct ctactacacc gccgacaaag gcggcttggg catagacaaa
      421 accctcgccg gcggcattgt cggcgcatac agcggcagcg tgtacctgtc caccattttg
      481 ggggcgtggt ttgccgaccg agtatggggt gcggaaaaaa ccctcttcct ctcgggcatc
      541 gtcgtgatgc tcggacacat cgtccttgcc gccgccccgg gcctgtacgg ccttttaatc
      601 gggctgatat tcatcgcatt gggcagcggc ggcgtgaaat ctacggccag ttctatggtg
      661 ggcgcattat acgaacagga cgaaatgcgc ccgctgcgcg atgcgggatt ttccattttc
      721 tacatcgcca tcaatatcgg cggcttccta ggcccgctgc tgaccggcct gctgcaggaa
      781 aacatcggtt tccattatgg tttcggcgcg gcggcggtcg gtatggcatt cggcttgtgg
      841 cgttattcct tgggacgtaa aaacctgccc catcccatcg tcccccatcc gctttcaaaa
      901 ggacagggca aaactgcggc cgccgtcggc attgctctca tcgccgcact tgcaaccgcc
      961 atcaaaaccg ggcttgtcaa cctcgacaat ttctccggca tcctcttatc taccgtcatc
     1021 cttgccgtca ttgcctattt tgcccgcctg ctgactaatc cccgcgtcag ttccgacaac
     1081 aaacggcaca tcatcgccta catcccgctt ttcctgacca tctgtatgtt ttgggccgtc
     1141 tggtttcaga tttacaccgt agcaaccgtc tatttcgacg aaaccgtcaa ccgcaccatc
     1201 ggttcgttta ccgtgcccgt cgcctggaaa gattctatgc aaagcctgtg ggtcatcctg
     1261 ttttccggac ttatggcggc aatgtggaca aaaatggggc gcaaacagcc caaaaccccg
     1321 ctgaaattcg ctatggcggt atttgttacc ggcgcgtcgt ttttgggatt catccccttt
     1381 atttccgccg gcacgccgat gcctattgcg gttttcgcac tgattgtcct tgccatcacg
     1441 ataggcgaac tgatgatttc cccgattgcg ctgtccatct ccaccaaaat cgcaccgcct
     1501 ttattcaaaa cccaaatggt cgcccttaat ttccttgcct tttcattagg cttcactttg
     1561 ggcggcgtat tgtttgaaaa atgctatcag gcgggcgacg aaatcggctt ctatcggctg
     1621 ctgttctaca tcggcgcagc cacaggcttc ctgctgctcc tgctcgtccc caaattgaac
     1681 aaaatgctcg aaggcacaga ctaagtcccg ccccgatgcc gtctgaaccc ttcagacggc
     1741 atttttccgc ataatgaaac caaaccgttt ccacccgaca ggacaggctc cccccaaccg
     1801 gaaggcagcc tgccgattgt catttaaata tctgactgat tccgtctgtt tgcaagggaa
     1861 atagccgttt atttccgctt gggcggaaac cggttctatt gagtaaaagg cattttatcc
     1921 gactaaatta gtgctgcatc aacggaa
//
LOCUS       DAWEWP010000215         1944 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWEWP010000215 DAWEWP010000000
VERSION     DAWEWP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.215"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(42..365)
                     /locus_tag="WGN53_001952"
     CDS             complement(42..365)
                     /locus_tag="WGN53_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0140886.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKARLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(353..592)
                     /locus_tag="WGN53_001953"
     CDS             complement(353..592)
                     /locus_tag="WGN53_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ0140887.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(715..1512)
                     /gene="truA"
                     /locus_tag="WGN53_001954"
     CDS             complement(715..1512)
                     /gene="truA"
                     /locus_tag="WGN53_001954"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ0140888.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESVPVRSPLLKNRAGWTHLKLDIGPMRRATALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          436 a          460 c          551 g          497 t
ORIGIN      
        1 ttaatccact ataatgaaca caatcaattc agactattca atcaggcaaa catctcctgc
       61 aatactgcaa acagtttttc agccgtactg ttgtctaaat ttccaagatg cttgaccaat
      121 ctggctttat ccacagccct aatctgttcg ggcaaaagca aaccgtcttt atcctgaaag
      181 cggacattga cgcggaacgg ggcaggacgg cttccgctcg tcataggaac gatcagcaca
      241 gtcttgagat agttgtgtat ttcaggagga gatactacga cacaaggacg tgtctttttg
      301 atttcgcttc ctacggtcgg gtctaaggag accagataga ttccgccgcg tactaccata
      361 tccattcttt atccgcttcg ttttcaattt cggaaaaaaa atgctcctgc tcggtttcga
      421 caagcattgc ggcagcttct gcccatcccc tgcgaacggt aggacagctt aaaatgatat
      481 tgcccttttc aactgtaaca gccaagctgt ctactgcccc tatttgaccc aataatgatt
      541 tgggcagaat cacgccttgc gagtttccca ttttgcgtat gttgagaatc atatacgtac
      601 catattcacc agtttatgta ataacaatgt tagtaccttg atgaggtagt gtcaacattg
      661 aaaaagattg cctgacagtt tgtccgattt caaaatctcc gcgacaagaa tgttttaaag
      721 ccattcgggg atttgggggc ggatgatgcc gtatgcctcg ggatagtcga cgccggtcag
      781 gtaaagtccg tcgggcatga aggtcggcgg ggctttgagg cggctgcgtt cttgaatcag
      841 tgcggcgaag ccttcgacgc tgagtctgcc gctgccgaca tagacgagcg cgcccatgat
      901 gttgcgtacc atatggtgca aaaaggcgtt gccgtgcaaa tcgaggcgga cgagtcctga
      961 gctttgggta aggtcggcgc ggtagatggt tttgacgggg gattttgcct ggcattcggc
     1021 ggcgcggaag ctggagaagt cttgttcgcc gatcaataag gcggtagccc ggcgcatcgg
     1081 cccgatgtcg agtttgaggt gtgtccagcc tgccctgttt ttgagcaggg gggaacggac
     1141 ggggacggat tcgagcaggt agcggtaatg ccgtccgtat gcgtcgaagc gggcgtgaaa
     1201 ttcgggggcg acctgtcggg cgtgcaaaac ggcaatgcct tcgggcaggt gggcatttac
     1261 gccgcgcacc catgcctgtt ggggacgggc ggcagttgtg tcgaagtgga cgacttgggc
     1321 ggtggcatgc acgccggtgt cggtcctgcc ggcaacggtg gtggaaaccg cttcccctgc
     1381 tatttgggcg agcgcggttt ccaatgccgc ctgaacggtc ggtacgccgt cagcctgttt
     1441 ctgccagccg taaaagcggc tgccatcata ggatagggtt attgcccagc gttgtttttg
     1501 tgcggtatcc atcggatttg ggattcggat aaatgttcag actgcattgt atcgcagatt
     1561 ttgcagggaa acggcaaatg cccggggcgc gcggcgttgt ttggggagtt ggggggggga
     1621 tgcagttgct acgaatcgct atcctgtgaa ttcaccctgt caggagtgcc cgaatcgtca
     1681 ttcccgcgca gtcgggaatc taggacgtaa aatctaaaga aaccatttta tagtggatta
     1741 acaaaaacca gtacggcgtt gcctcgcctt agctcaaaga gaatgattct ctaaggtgct
     1801 gaagcacgag tgaatcggtt ccgtattatt tgtactgtct gcggcttcgt cgccttgtcc
     1861 tgatttttgt taatccacta tatccgataa gtttccgcac cgacaaaact agattcccac
     1921 tttcgtggga atgacgggat gcag
//
LOCUS       DAWEWP010000216         1831 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWEWP010000216 DAWEWP010000000
VERSION     DAWEWP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.216"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            54..1223
                     /gene="metZ"
                     /locus_tag="WGN53_001955"
     CDS             54..1223
                     /gene="metZ"
                     /locus_tag="WGN53_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ0140889.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVANLEALAELAHGIGAILVVDNSLLSPIGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1309..1515
                     /locus_tag="WGN53_001956"
     CDS             1309..1515
                     /locus_tag="WGN53_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ0140890.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT          440 a          488 c          474 g          429 t
ORIGIN      
        1 taaacaacgg gcgcacgctc cgttgccgaa cggataaaac cgaggaatta ccgatgagca
       61 aaaaactcca tccgcaaacg ctcgccatac gcggcggcaa agaacaaacc gaataccgcg
      121 aacacaatca ggccttgttc ctgaccagca gcttcatgtg ggacaacgcc caacacgccg
      181 ccgatttgtt ttcaaaaaaa atcaaaggct tcacttatac ccgtaccgcc aacccgacca
      241 cagccgcctt tgaaaaacgc atcgccgctt tggaaggtgc ggaacgcgcg gttgccactt
      301 cgaccggcat gtccgcgatt caggcggcgt ttttcacctt tttgcaggcg ggcgaccatg
      361 tggtttccag ccgcagcctg ttcggcacga ccgtcggctt tatcaataac atcgttacca
      421 aattcggcat cgaagtgagc cacgtgtcgc cgactgatat aaacgaatgg aaagccgccg
      481 tcaaagccaa taccaaactg ctgtttttgg aaacgccgtc caaccccttg ggcgaagtgg
      541 ccaacttgga ggcattggcg gaattggcgc acggcatcgg cgcgattttg gtggtggaca
      601 acagcctgct gtcgcccatc ggctctcagc ctttgaagca cggcgcagat atttccgttt
      661 cttccgcaac caaagccata gacggacacg gacgcgtgat gggcggcgtg ttggcgggtt
      721 cggaagaact gatggcgcag gccgccgttt actgcaactc ttgcgggctg gcgatgtcgc
      781 cgtttaacgc gtggcagttg ttgagcggcg tggaaaccct gtcgctgcgt atggaaaaac
      841 agttcgacaa cgccctgaaa atcgcacaat ggctgcaagc gcagccgcag gttcaagccg
      901 tgtactacac cggattgccc gaccatccgc aggcggcgct tatccgcaaa cagcaaaacg
      961 gcggcggcat cgtcatcggc tttgaagtcg ccgaccaagc agccgcgtgg aaagtcgtgg
     1021 acggcgtgga actcttttcc cgtaccgcca acctcggcga cgtgcgctcg accatcaccc
     1081 atccgtggac aaccacgcac ggcaggatgc agcccgaaga aaaactcgcc gccaacatcc
     1141 gccccggatt ggtgcgcctg tcggtcggtt tggaatatgt tggcgatttg attgacgatt
     1201 tgaaacaggc actcgcacgc tgattgcgtg tttcagacgg cattggacag cagatgccgt
     1261 ctgaaaccct tatcgcgtat ggcatacaca tcaacaatag gaaaaaatat ggatgaagcg
     1321 gtttttgccg attgggcgtt gaaaatctgc ctgaccggtt tgattatttt tttgggtttt
     1381 atcgtttgga atttgggcaa ggagtccaaa gcgggcaaat tcggcatcgc cattttgttt
     1441 ttggttttgg gcttgggcgt gttcggtttt gtatttaaag aactgctaat caagtttttg
     1501 gtattgccca aataggctgc cgcctgtcaa aatgctgtct gaaatcataa aaagtaatct
     1561 gcaccctaaa agtttgattt aaccgccaac tgattaaggt gcggattttt atgacaaaat
     1621 aaaaatcatc tttccgcagt cattcccacg gacgcgaaag tctgtctttt tgaatcccgg
     1681 ataattttac atcgtcattc ccgcgcaggc gggaatctag tctgttcggt ttcggtcatt
     1741 tacgataaat tcctattgct tttcatttcc tgattcccac ttccgtggga atgacggcgg
     1801 agcggataaa ttctcgcaat ctaaaacctc g
//
LOCUS       DAWEWP010000217         1828 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWEWP010000217 DAWEWP010000000
VERSION     DAWEWP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.217"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..728)
                     /gene="rfbA"
                     /locus_tag="WGN53_001957"
     CDS             complement(<1..728)
                     /gene="rfbA"
                     /locus_tag="WGN53_001957"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0140891.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQIKPSARGELEITTLNQMYLDDGSLNVQLLGRG
                     FAWLDTGTHASLLEAASFVETIEN"
     gene            complement(791..>1828)
                     /gene="rfbB"
                     /locus_tag="WGN53_001958"
     CDS             complement(791..>1828)
                     /gene="rfbB"
                     /locus_tag="WGN53_001958"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0140892.1"
                     /translation="GFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESLTDIADNPRYA
                     FEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAA
                     RAYWQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPYSASKVSSDHL
                     VRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLF
                     VEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYED
                     LITFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQWYLDNKTWWQNVLNG
                     SYRLERLGTGK"
BASE COUNT          411 a          445 c          527 g          445 t
ORIGIN      
        1 atgttttcaa tggtttcgac aaacgaggct gcttccaaca ggctggcgtg tgtgccggta
       61 tcaagccatg cgaaaccgcg accgagcaat tggacattga gcgaaccatc gtccaaatac
      121 atttgattca aggtggtaat ttccaattcg ccgcgtgcgg acggtttgat ttgtttggcg
      181 aactcgacgg cacggttgtc gtagaagtac aagccggtta ccgcccaatc ggatttgggc
      241 cgttgcggtt tttcttcgat ggaaacggcg cggaagtttt cgttaaattc aaccacgccg
      301 aaacgttcgg ggtttttgac ctgataagca aacacggttg cgccgtgcgt ttgcgctgcc
      361 gcctgtttca atgtttgcgt aaacgactga ccgtaaaaaa tattgtcgcc caaaaccaag
      421 caaacattgt cgttgccgat aaattcttcg ccgatgataa atgcctgtgc caagccgtcc
      481 ggactgggtt gcacggcata actgatggaa atgccgaaat cgctgccgtc gccaagcagg
      541 cgtttgaaag aggcgttgtc ttcaggcgcg gtaatcacca aaatatcgcg gattcccgcc
      601 agcatcaaaa ccgacaaggg gtaataaatc atcggtttgt cgtacacggg caggagctgt
      661 ttggatacgc cgcgcgtgat ggggtagagg cgcgtgccgc tgccgcctgc cagtatgatg
      721 cctttcatct ttgttttctt cctttgcgat gggttttcag acggcattgc gtcgggatgc
      781 cgtctgaaaa ctattttcca gtacctaaac gttccaaacg atagctgccg ttcaatacat
      841 tttgccacca ggttttgttg tccagatacc attgcacggt tttgcggagg ccggactcga
      901 aggtttccaa aggcagccag cccaaatccc gcctgatttt ggctgcgtcg acggcgtagc
      961 gtacgtcatg gccggggcgg tcttgtacga aagtaatcaa atcttcataa cgcgccacac
     1021 cggccggttt ttcgggagcg agttcttcca gcagggcgca gatggttttg acgacttcaa
     1081 tattggcttt ttcattgtgg ccgccgatat tgtaggtttc gccgacaaca ccttcggtaa
     1141 caacctgata cagtgcgcgc gcgtggtctt cgacaaacag ccagtcgcgg atttgcatac
     1201 cgtcgccgta cacaggcagc ggtttgccgt caagcgcgtt cagaatcatc aaaggaatga
     1261 gtttttccgg aaaatggtaa ggaccgtagt tgttggagca gttggttaca atggtcggca
     1321 agccgtaagt acgcaaccac gcgcggacga ggtggtcgct ggacacttta gaggcagagt
     1381 aggggctgga cggcgcgtag ggcgcggttt cggtaaacaa atcgtccgtg ccgcctaaat
     1441 cgccatagac ttcatcggtg gaaatatggt ggaaacggaa ggcttcgtgc tgttcagacg
     1501 gcatttgttg ccagtaggcg cgggctgctt caagcagatt gaatgtgccg acgatattgg
     1561 tttggataaa ctcgcctgcc gaaccgatag agcggtcgac atggctttcc gccgccaagt
     1621 gcatcacggc atcgggccgg tgttgtgcga atacgcggtc gagttcggcg cggtcgcaaa
     1681 tgtccacttg ctcgaaagca tagcggggat tatcggcaat gtcggtcagc gattcgagat
     1741 tgcccgcgta ggtcagtttg tcgaggttga cgacggaatc ttgggtgttt cggataatat
     1801 gacggacaac ggcagaaccg ataaagcc
//
LOCUS       DAWEWP010000218         1800 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWEWP010000218 DAWEWP010000000
VERSION     DAWEWP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.218"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            97..525
                     /locus_tag="WGN53_001959"
     CDS             97..525
                     /locus_tag="WGN53_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0140893.1"
                     /translation="MGNFAKVSGYTYNKSRYRNAAEVNAERLAKNSSADPYNFSNFTP
                     VHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNIRQGGYGLIDGFVRYELGKHAKLS
                     LIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            669..1727
                     /gene="dinB"
                     /locus_tag="WGN53_001960"
     CDS             669..1727
                     /gene="dinB"
                     /locus_tag="WGN53_001960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ0140894.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLIARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT          427 a          485 c          495 g          393 t
ORIGIN      
        1 cagtcgatgt gcttggcaat catggcttgg gcggtttgcc ggaagaaggt gctcatgaga
       61 aatcccctaa atgtcttggt ggaaatttag gggattttgg ggaattttgc aaaggtctca
      121 gggtatacct acaataagag caggtatagg aatgcagccg aagtcaacgc cgaacgcctt
      181 gccaaaaatt ccagtgcaga cccgtacaac ttcagcaatt tcacacccgt gcacatattc
      241 cgtttcggaa cgagcttcca tataccgaat acggggctga ccgtcggcgg cggcgtgtcc
      301 gcacaaagcg gcacaagcag cctgtacaac atccgtcagg gcggctacgg gctgatagac
      361 ggtttcgtcc gttacgaatt gggcaaacac gccaaattga gcctcatcgg tacgaactta
      421 aacggacgca cttattttga gaacaactac aaccgtacgc gcggcgcaaa caacttctat
      481 ggagagccgc gcactgtcag catgaaactg gattggcagt tttaattgaa aaccgtagtt
      541 ttgcggatgc cgtctgaaac agggtatgtt tcagacggca tttttatgga ggcaggccta
      601 tcggggcgta tatttggaat ttgcccgatg ccggcagtag aatatccctt ttatcctcag
      661 aagagccgat gtcttcacgc aaaattatcc acatcgacat ggacgcattc tacgcatcgg
      721 tagagctgcg cgaacagccg catttgaaag ggcggccggt ggtcgtcgcg tgggagggcg
      781 cgcgttcggt gatttgcgcc gcatcgtatg aggcacggca gttcgggctg cattccgcga
      841 tgtcggtggc aacggcgaaa aggctgtgtc cgcaagcggt gtatgtgccg ccgcatttcg
      901 atttgtaccg tcaggtttcc gcgcagattc acgccgtatt caggcgttat accgatttaa
      961 tcgaaccctt gtcgctggac gaagcctatc ttgacgttac ccgtaatttc aaaaacatcc
     1021 cttacgccag cgaagttgcc aaagaaatcc gtgccgccat ttttgcggaa acaggtttga
     1081 ctgcatccgc aggcatcgcg ccgaacaaat ttctggcgaa aatcgcgtcg gactggcgca
     1141 agccgaacgg gcagtttgtg ttgccgccgc acaaagtcat ggcatttttg gaaaccctgc
     1201 ctttgggcaa aatccccggc gtgggcaagg taacgctgaa aaaaatgcag tcgctgggta
     1261 tgcggacggc gggcgacttg cgccgtttcg agcgcggcga actcttaaac catttcggac
     1321 gctacggata ccgcctctat gatttggtgc gcggtacgga tgaacgcccc gtcaaagccg
     1381 aacgcgaacg cctccaaatc tccacagaaa tcaccctgcc tgaagacctg ccgctcgagc
     1441 aggctgccgg acacctgccc catcttgctg aagacctgtg gcggcaaatc acgcgcaaaa
     1501 acgtcgaagc ccaaagcgta acgctcaagc tgaagaccta cgatttccgc atcatcacgc
     1561 gcacactgac ttattcctcc gtattgcccg actgcgcagc tctgctgcag gctgcgcaaa
     1621 tgttgatagc gcgcgtcccg ccgcagacgg aagacgcgtt ccgccttatc ggtatcggcg
     1681 tggggcatct tgtgccgaaa aaccagcagc aggatttgtg ggcgtaaacc gctttacgcg
     1741 cgccgtccaa aaaacatgcc gtctgaacgg ttttcagacg gcatggctgc tttatagtgg
//
LOCUS       DAWEWP010000219         1793 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWEWP010000219 DAWEWP010000000
VERSION     DAWEWP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.219"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            52..1560
                     /gene="nadB"
                     /locus_tag="WGN53_001961"
     CDS             52..1560
                     /gene="nadB"
                     /locus_tag="WGN53_001961"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ0140895.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASHH
                     AQGGIAAAWSREDDIEKHVADTLEAGAGLCNEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NYDGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLDCRTQETHRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISHQPAAFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQTVPSKRSSENTSAEADIFSDGIQNTFSRPALQAFNQRHLGILRNDTGLRR
                     AIAQLRLWKQNQTEPHTVSEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT          432 a          603 c          445 g          313 t
ORIGIN      
        1 aatttaatcc actataccgt ttcagacgac ctctcaaaca aggacaacac catgcaaacc
       61 gattgcgacg tattgattgc cggaaacggg ctggcggcac tgacgctcgc cctgtcgctg
      121 cctgaatcgt tccgcatcgt cattttgtgc aaaaaccggc tggacgacac cgccagccat
      181 catgcgcaag gcgggattgc ggcggcgtgg tcgagagagg acgacatcga aaaacacgtt
      241 gccgatactt tggaagcggg cgcaggtttg tgcaacgaag ccgccgtccg caccatcctg
      301 tcgcagggca aaccggcaat cgaatggctg ctggcgcagg gcgtggcgtt cgaccgaaat
      361 tatgacggtc tgcacctgac acgcgaaggc gggcacacct gccgccgaat cgcccacgtc
      421 gccgactaca cgggcgaagc cgtcatgcag agcctgattg cccaaatacg ccgccgcccg
      481 aacatccgcg tttgcgagcg gcagatggcg ttggacattc aaaccgaatc aggcgcggcg
      541 tgcggactga ccgtcctcga ctgccgaacg caagaaaccc accgcatccg cgcccgccat
      601 accgtactcg caggcggcgg cttggggcag atttacgccg ccaccaccac gccgcccgaa
      661 tgcacgggcg acgccatcgc tatggcgata cgcgcaggct gcgcagtcga aaacctcgaa
      721 tttatccaat tccaccctac aggcttggca aggccgtctg aaaacggccg caccttcctg
      781 atttccgaag ccgtgcgcgg cgaaggcggc atcctgacca accaagcggg cgaacggttt
      841 atgccgcatt acgaccgccg cgccgaactc gcaccgcgcg acatcgttgc ccgcgccatt
      901 gccgccgaaa ccgccaaaca aacgcaagac ttcgtctcgc tcgacatcag ccatcaaccc
      961 gcagcgttcg tccgccagca ttttccgtcc atccatcgac actgtttatc ccaatgcggc
     1021 ttggacatca cgcgccaagc catccccgtc cgccccgtgc aacactacac ctgcggcggc
     1081 atccaaaccg accccagcgg cagaacttcc ctgccgcagc tctacgcctt aggcgaaacc
     1141 gcctgcacag ggttgcacgg agccaaccgc ctcgccagca actccctact cgaatgcgtc
     1201 gttaccgcca ggcttgccgc ccaaaccatc gcggatggac aagcgttcca aaccgtaccg
     1261 tccaaaaggt cgtctgaaaa cacctccgcc gaagcagaca tcttttcaga cggcatccaa
     1321 aacacattca gccgccccgc cctgcaagcg ttcaaccaac gccatctcgg catcctccgc
     1381 aacgataccg gcctgcgccg cgccatcgcc caactgcggc tttggaagca aaaccaaacc
     1441 gaaccgcaca ccgtgtccga atacgaaaac cgcaacctgc tcgaatgcag cctcgccgtc
     1501 gcccaagccg catacaggcg gagacagaac atcggcgcgc attttaatag tgattgttaa
     1561 ggttggctgg ttagaaaaaa tcgttcaaat gaattttaat agcatggtta agagattggc
     1621 attcagtttt tagggttgcg taagttgggt tatcaatctg ttctaaaagc agaaaggccg
     1681 agacctttgc aaaattcccc aaaatcccct aaattcccac caagccattt aggggatttt
     1741 ccatgagcac cttcttccgg caaaccgcac aagccatgat tgccaaacac atc
//
LOCUS       DAWEWP010000220         1776 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWEWP010000220 DAWEWP010000000
VERSION     DAWEWP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.220"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..765
                     /gene="mafB"
                     /locus_tag="WGN53_001962"
     CDS             <1..765
                     /gene="mafB"
                     /locus_tag="WGN53_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0140896.1"
                     /translation="ALGIGDILYGTRYAIDKAAMRNIAPLPAEGKFAVIGGLGSVAGF
                     EKNTREAVDRWIQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDFSAAYNT
                     RTTRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPNHINVLSGNSIQHILYGDEAGGG
                     HLFPGKPGKTTFPQHWSASKITHEISDIVTSPKTQWYAQTGTGGKYIAKGRPARWVSY
                     ETRDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK"
     gene            780..1010
                     /locus_tag="WGN53_001963"
     CDS             780..1010
                     /locus_tag="WGN53_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0140897.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            1159..>1776
                     /locus_tag="WGN53_001964"
     CDS             1159..>1776
                     /locus_tag="WGN53_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HEZ0140898.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLYSESGKTQTVRNTVV
                     KPKPLKAYNLTVADWHTYFVKGNRAETEGVWVHNECPPRKTPSTPIYGNDSEAYAAAK
                     ELGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPEKL"
BASE COUNT          615 a          377 c          387 g          397 t
ORIGIN      
        1 gccttgggca taggcgacat actgtacgga acgcgctatg ccatagacaa agccgcaatg
       61 cgcaacatcg cccccttgcc cgccgagggc aaattcgccg tcatcggcgg cttgggcagc
      121 gtggcgggct ttgaaaaaaa tacgcgtgaa gccgttgacc ggtggataca ggaaaacccc
      181 aatgccgccg aaaccgtcga agccctggtc aacgtcctgc cgtttgccaa agtcaaaaac
      241 ctgacaaagg cggcaaaacc ggggaaggct gcggttagcg gggatttttc tgctgcatac
      301 aatacaagaa caactagaaa agttactaca gaaacagagg ggttaaatag aatcagacag
      361 aaccagaaaa atagtaatat acatgagaaa aattatggaa gagataatcc taatcatatt
      421 aatgttttat ctggaaattc tatacaacat atactgtatg gagatgaagc aggaggtggg
      481 catctttttc ctggcaaacc tggtaagaca acattccccc aacattggtc agccagtaaa
      541 ataactcatg aaattagtga tatcgttaca tccccaaaaa cgcaatggta tgcacagact
      601 ggaacaggcg gcaaatatat tgctaaagga agaccagcca ggtgggtatc atatgaaacg
      661 agagatggaa ttcgtatcag aacagtttat gaacctgcaa caggaaaagt ggtaactgca
      721 ttccccgata gaacctctaa tcccaaatat aaccctgtaa aataatgagt atttgaaaga
      781 tgaaaacatt agacgaacga ataaaaaatt taggaaaatc tcttgaagat agaattgacg
      841 ctaatctaat tgatgccgca ctagagtaca ttactttttc agaacgtttg ctagcttttg
      901 aaacgctatg tgattatata gaagatttca atatccagct tacggaaaaa gaatcccaag
      961 aaatctcttt tattaataag gaatttggaa ttgaaagtac atcagattaa taaatattag
     1021 gtcggtttaa aaacccaaca atcaaaaagt cgtctgaaac tgtgtttaat ttacagacga
     1081 cctttccttc atttgaagca ggatattgag aactgagttc ttcaaaaatc ctacacctgc
     1141 tccttccacg gcagcacctt ggtcaaaacg gcagacggct acaaagccat tgcccatatt
     1201 caagccggag accgcgtcct ttccaaggac gaggcaagcg gagaaacggg atacaaaccc
     1261 gttaccgccc gatacggcaa tccctatcaa gaaaccgttt acattgaaat ttcagacggc
     1321 atcggcaaca gtcaaaccct gatttccaac cgcatccatc cgttttattc ggacggcaaa
     1381 tggattaagg cggaagattt aaaagcggga agcaggctgt attccgaaag cggcaaaacc
     1441 caaacagtcc gcaacaccgt tgtcaaacca aaaccgctca aagcctacaa tctgaccgtt
     1501 gccgattggc atacttactt tgtcaagggc aatcgggcgg aaacggaagg ggtttgggtt
     1561 cataatgagt gtccgccaag aaaaacacca tctaccccca tatatgggaa tgattcagaa
     1621 gcatatgcag cagcaaagga attgggatat agaaaaataa aagaaagaac gagaaatgat
     1681 gctgctattt ttaaaaaagg aaagagttat atatcacgcg atgtagattc acataatgga
     1741 ggtgcttgga aagaggcatc ttcacccgaa aaactg
//
LOCUS       DAWEWP010000221         1747 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWEWP010000221 DAWEWP010000000
VERSION     DAWEWP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.221"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            35..922
                     /gene="prmA"
                     /locus_tag="WGN53_001965"
     CDS             35..922
                     /gene="prmA"
                     /locus_tag="WGN53_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ0140899.1"
                     /translation="MPYQQITINVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            940..1503
                     /gene="orn"
                     /locus_tag="WGN53_001966"
     CDS             940..1503
                     /gene="orn"
                     /locus_tag="WGN53_001966"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ0140900.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESLHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPSVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
BASE COUNT          468 a          538 c          433 g          308 t
ORIGIN      
        1 aattcaatcc actatatttc caagaaagcc cgttatgccc taccaacaaa tcaccatcaa
       61 cgtcaacgat gccgtcgccg aacgcctcgc cgacgcgctg atggaacacg gcgcactctc
      121 cgccgccatc gaagatgcct acgccggcac gcaaaacgaa caggcgattt tcggcgaacc
      181 cggtatgccc gccgaacaaa tctggcagca gagcaaagtc atcgccctgt tcggcgaaca
      241 cgacgaagcc gccgccatca tccaaatcgc cgcacaagaa tgcgggttaa aagacttggc
      301 atacaccggc gaaaccatcg aagaccaaga ctgggtgcgt ctcacgcaat cgcaattcga
      361 ccccatccga atttccgacc gcctgtggat taccccctct tggcacgaag cccccgaagg
      421 cactgccgtc aacctacgcc tcgatcccgg cctcgccttc ggcactggca gccacccaac
      481 cacgcgcctc tgcctcaaat ggctggatac gcaactcaaa aacggcgaaa gcgtcctcga
      541 ctacggctgc ggttcgggca tcctgaccat cgctgccctc aaactcggcg caggttctgc
      601 cgtcggcgtc gatattgacg aacaagccgt ccgcgccggc aaggacaacg ccgaacaaaa
      661 caacgtcgat gcacaattct tcctgcccga cggcctgcct caagggcaat tcgacgtagt
      721 tgtcgccaac atcctcgcca accctttacg tatgctcggc gaaatgctcg ccgcccgcac
      781 caaacaaggc ggccgcatcg tgttatccgg tttgttggac gaacaagccg aagaactcgg
      841 cggcatttac agccaatggt tcgatctcga cccggcggaa accgaagaag gctgggcgcg
      901 cttgagcggg acaaaacgct gaaacggaaa ggaaacaccg tgcaggacaa aaacaacctc
      961 tgctggctcg atatggaaat gacggggctt aatcccgaaa ccgaccgcat tatcgaagtc
     1021 gcgatgatta ttaccgactc ggatttgaat gtattggcgc aatccgaagt ttacgccgtc
     1081 caccaaagcg acgagctgct cgacaatatg gacgaatgga acaccgccac acacggtagg
     1141 acggggctga cacagcgcgt gcgcgaatcg ttgcataccg aagccgaagt cgaacagaaa
     1201 ctgctggact ttatgtcgga atgggtaccc ggacgcgcca cgccgatgtg cggcaactcc
     1261 atccaccaag accggcgttt tatggtcaaa tatatgccga aactggaaaa ctacttccac
     1321 taccgcaacc tcgacgtttc cacgctcaaa gagcttgcca aacgctggaa tccgtccgtt
     1381 gccaaaagcg tcgtcaaacg cggttcgcac aaggcattgg acgacatttt ggagagcatc
     1441 gaagaaatgc gccactaccg cgaacacttt ctgatttccg ccccgagagc cgaagcgcaa
     1501 taagaaacaa aaaatgccgt ctgaagcgca aatcctgcat ttcagacggc atttttacag
     1561 cagattgaaa tcaaaaatat acacgcccgt cattcccgcg caggcgggaa tccggaaggt
     1621 cgggcctgcc gttattttca atcattacag aaactgaaag gtctggattc ccgcctgcgc
     1681 gggaatgacg ggcgtgtgca ttcttatagt ggattaacaa aaatcaggac aaggcgacga
     1741 agccgca
//
LOCUS       DAWEWP010000222         1746 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWEWP010000222 DAWEWP010000000
VERSION     DAWEWP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.222"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            97..1470
                     /locus_tag="WGN53_001967"
     CDS             97..1470
                     /locus_tag="WGN53_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0140901.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1650..>1746
                     /locus_tag="WGN53_001968"
     CDS             1650..>1746
                     /locus_tag="WGN53_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEZ0140902.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYSGLN"
BASE COUNT          447 a          565 c          412 g          322 t
ORIGIN      
        1 gccgccttgt cctgattttt gttaatccac tatatttccc cgtcctatcg gtttcccgtt
       61 tcagacgaca taaggtctga aagaaagact acaattatga gtaatccatt ttcctcttta
      121 ggtttgggta cggaactcgt ttccgcactg accgcgcaag gttacgaaaa cccgacgccc
      181 atccaagccg ccgccattcc caaagcactc gccggtcatg atttgctagc cgccgcgcaa
      241 accggcacag gcaaaaccgc cgcctttatg ctgcccagtc tggaacgcct caaacgttac
      301 gccaccgcca gcacctcgcc cgcgatgcac cccgtgcgta tgctcgtcct tacccccacg
      361 cgcgaacttg ccgaccaaat cgaccaaaac gtgcagtcct acatcaaaaa cctgccgctt
      421 cgccacaccg tcttgttcgg cggtatgaat atggacaaac agaccgccga tttgcgtgcc
      481 ggctgcgaaa tcgtcgtcgc caccgtcgga cggctgctcg accacgtcaa acaaaaaaac
      541 atcagtttga acaaagtcga aatcgtcgtt ctggacgaag cagaccgcat gttggatatg
      601 gggtttatcg acgacatccg caaaatcatg cagatgctgc ccaaacaacg ccaaaccctg
      661 ctcttttccg ctaccttctc cgcaccgata cgcaaactgg cgcaagactt catgaacgcg
      721 cccgaaaccg tcgaagtcgc cgcgcaaaac accaccaacg ccaacgtcga acaacacatc
      781 atcgccgtcg ataccattca gaagcgcaac ctgctcgaac ggctgattgt cgatttgcat
      841 atgaaccagg tcatcgtgtt ctgcaaaacc aaacaaagcg tcgaccgcgt aacgcgcgaa
      901 ctggtgcgcc gcaacctgtc cgcacaggcg atacacggcg accgttccca acaaagccgg
      961 ctcgaaacac tcaacgcctt caaagacggc agcctgcgcg tcctcgtcgc caccgacatc
     1021 gccgcgcgcg ggctggacat tgccgaactg cccttcgtca tcaattacga aatgcccgcc
     1081 cagcccgaag actacgtcca ccgcatcggg cgcacggggc gcgcgggcgc ggacggcgtg
     1141 gcgatttccc tgatggacga atccgaacag aaaatgtttg aatccattaa agagctgacc
     1201 ggcaacaagc tgctcatcga gcgcatcgag ggcttcgagc cgcaatggtg ggaacagggc
     1261 ggcgcaaaac cggaaaaaac cgaaatgcgc gaaccgagac aacgcaaccg ctacgaatcc
     1321 gccagggctc agcgcgaaaa aaatacccga ccggaaacca cggcaaacga tgcgggcgcg
     1381 gcttgcggaa aaattgccgg acgcagccgc cgaagccgtc gggaacaccg gacgtgcgcc
     1441 ctgctccaac cgcgttacgg cgtaaaataa ccctgaaaat caaatgccgt ctgaacattt
     1501 cccgtttcag acggcatttt tcaaaccgga ctgacgcatt gggagcaacc gcccgcaccg
     1561 gataaatttc ttccgcaaac agtttcagac ggcatttgct gcctgtacaa tatcatttcc
     1621 ttttccactc aaaccatcag gatttcccaa tgtccaccca attcgatgtc gccgtaatcg
     1681 gcgcaggtcc ggcaggctcg gtcgcatccg ccctgctccg caaaaaaggt tatagtggat
     1741 taaatt
//
LOCUS       DAWEWP010000223         1735 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWEWP010000223 DAWEWP010000000
VERSION     DAWEWP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.223"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            356..1660
                     /locus_tag="WGN53_001969"
     CDS             356..1660
                     /locus_tag="WGN53_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ0140903.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          356 a          433 c          513 g          433 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cggcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggga tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggagcgcatt gggtgtgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggttat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taaac
//
LOCUS       DAWEWP010000224         1732 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWEWP010000224 DAWEWP010000000
VERSION     DAWEWP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.224"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(111..767)
                     /locus_tag="WGN53_001970"
     CDS             complement(111..767)
                     /locus_tag="WGN53_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ0140904.1"
                     /translation="MKNKSKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALVAAAILLLS
                     "
     gene            832..1722
                     /locus_tag="WGN53_001971"
     CDS             832..1722
                     /locus_tag="WGN53_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ0140905.1"
                     /translation="MFPVFHLSGESRRRMLQAASRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMRGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWMVGCGLGTDKAAVGTLAGILAEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGVWLHGAAADVIKESAGIAAGL
                     LAGEISPAARWLRNRITESM"
BASE COUNT          384 a          413 c          575 g          360 t
ORIGIN      
        1 ttgggacgga aaaacggcct gaagccgttt cgggcattca gaccgtttgc gcggagaggg
       61 gatgccgtcc gaagggcgaa aagggcttca gacggcattg atgtcgggtt tcaggacagg
      121 agcaggatgg cggctgcgac aagcgcggca accgataatg cggcggcaag cgcggttttg
      181 ccggcaaagc ggattgaggt tttgccttcg atgtatttga agccggttat catcgggagg
      241 acgaggtttt tctttttgaa cacgcggtat gcggcgacgg cggcgatatg aatggcggaa
      301 aaaacggcga gcagcttgaa aaagttgagg tggattttcc gcataaggct gcccgtatgc
      361 tcggaaacca aatggttgag gtagccgttg gtgctgaagg tgttttcatc ggcggcaaaa
      421 agccctgtgc cgacttgaaa tgagacggcg gccaaaagcg cgacgaccat cagtgcgccc
      481 aaggggttgt gtccgggttg gacgtgttcg ggaataccgt ttttcatata ctctctgata
      541 cccgaccatc cgcggacgaa acgggagaaa cgggcggtat cgctgcccca aatgccccag
      601 cagaggcgga atacgagcag gaaaaggata aacagcccga cgcgcgtgtg ccattgcagc
      661 atatcgccgc cggttttcgc gctataccac ataaagggta gggatgcggc aagcagccag
      721 tggaaaaggc gggtggggaa gtcccagact ttgcttttgt ttttcataat cggtttccgg
      781 cggtagaatc ggtttgtttt cgagccttaa ttttaaccga ttggagggga aatgtttccc
      841 gttttccatc tttcaggcga aagccgccgc cggatgcttc aggcggcatc gcgttttccc
      901 tatgttttca aagcccgtgc ggaagattcg cacaaaggga ctttcggcac gctcgccgta
      961 gtcggcggat cggcagggat gagaggcgcg cccgtattgg cggcatcggc ggcgatgtat
     1021 ctcggctgcg gcaaggtgtg ggcgggtttc aatcaggatg cgcttccgtt tgccgtcatt
     1081 gccggttttc ccgaaattat gttggacacg gcagacggtt tgaccaagcg tcaaggcata
     1141 aacgcttgga tggtaggttg cggcctgggt acggataaag cggcggtcgg aacgcttgcc
     1201 ggaattttgg cggaacacac ggacaagccc gtccttttgg atgcggacgc gctgaacata
     1261 ttatcaaccg atgccgaaac ccgaaatctg gcgcgcgggt gtaaaaacct gattttaacg
     1321 ccacaccccg ccgaagccgc gcgcctgctt ggaacgacgg ttgcacaggt tcaggcggat
     1381 cggacggcgg cagtgaggaa gataagggca attttcggcg caaccgtggt tttaaagggg
     1441 cacaaaacat tggttgcctc acccgatacg gaaatctatg tcaacgaaag cggcaacgcg
     1501 ggattggcaa cggcgggcag tggcgacgta ttgggcggca tcatcggcag tctgctcgca
     1561 cagggcgtgc cggtttttga agccgcctgc gcgggcgtgt ggctgcacgg cgcggcggcg
     1621 gatgtcataa aagaatcggc aggcattgcg gcggggctgt tggctggcga aatctccccg
     1681 gcggcaaggt ggctgcgcaa ccggataact gaaagtatgt aagaagatat ag
//
LOCUS       DAWEWP010000225         1724 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWEWP010000225 DAWEWP010000000
VERSION     DAWEWP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.225"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..365
                     /locus_tag="WGN53_001972"
     CDS             <1..365
                     /locus_tag="WGN53_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0140906.1"
                     /translation="EYDENSFIGKWYDDGVWDDEEYWKLENDLIEVRKKYPYPMDIPR
                     DIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFRVMLRYIFTDLDVEDW
                     KFFYFPIQNSKGRLRRKV"
     gene            387..764
                     /locus_tag="WGN53_001973"
     CDS             387..764
                     /locus_tag="WGN53_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0140907.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRYVVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            800..1285
                     /locus_tag="WGN53_001974"
     CDS             800..1285
                     /locus_tag="WGN53_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HEZ0140908.1"
                     /translation="MRLSDGIGNSQTLISNRIHSFYSGGKWIKTEDLKAGIRLLSESG
                     KTQTVRNIVVKPKPLKAYNLTVADWHTYFVKGNRAETEGVWVHNACPPKRTGSSKNEK
                     HGDDGRSQISAESRIAELKNKIIPGMPKNERLKIEQKIKNITKNANRKAKGEEHGRRG
                     R"
     gene            1266..1721
                     /locus_tag="WGN53_001975"
     CDS             1266..1721
                     /locus_tag="WGN53_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140909.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVEKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
BASE COUNT          598 a          275 c          359 g          492 t
ORIGIN      
        1 aggagtatga cgaaaattca tttattggca aatggtatga tgacggggtg tgggatgatg
       61 aagaatattg gaaattggag aatgatttaa ttgaggttag aaaaaaatat ccttatccga
      121 tggatatacc aagggacatc gtgattggaa tcggtaccat tattgatttt ttaatggttc
      181 aaaattggaa actttttgaa attaaagctt ctccttggtt gcctaaaagt gtcaaaatta
      241 atgagcgtta tgaaaggttc agggtaatgc tccgttatat ttttactgat ctagatgtag
      301 aggactggaa atttttttac tttccaatac aaaatagtaa aggtagattg agacgaaaag
      361 tttaattata ttggagatac attattatgt gtgagttcaa ggatattata agaaacgttc
      421 cttattttga ggggtatgac gaaaattcat ttattggcaa atggtatgat gacggggtgt
      481 gggatgatga agaatattgg aagttggaga atgatttaat cgaggttaga aaaaaatatc
      541 cttatccgat ggacatacca agatatgttg tcattggaat cggtaccatt attgatttct
      601 taatggttcc aaattggaaa ctttttgaaa ttaaagcttc cccttggcta cctgatagcg
      661 tgggaattaa cgaacgttat gaaaggctta aaacaatgct ccgttatatt tttaccgaga
      721 aagacatagt caacgtgcga tttgattatt acaacaaaaa atagcaaaac ccggcaatca
      781 aaatgccgtc tgaagcgata tccggctttc agacggcatc ggcaacagcc aaaccctgat
      841 ttccaaccgc atccactcgt tttattcggg cggcaaatgg attaagacgg aagatttgaa
      901 agcgggaatc aggctgttat ccgaaagcgg caaaacccaa accgtccgca acatcgttgt
      961 caaaccaaaa ccgctcaaag cctacaatct gaccgttgcc gattggcata cctactttgt
     1021 caaaggcaat cgggcggaaa cggaaggagt ttgggttcat aatgcgtgtc cgcccaaaag
     1081 aacaggaagc tccaagaatg aaaaacatgg agacgacggt cgaagtcaaa tatcagcgga
     1141 atcaagaatt gctgaattaa aaaataaaat tattcccgga atgcccaaaa atgaacgctt
     1201 aaagattgag caaaaaatca aaaatattac aaaaaatgcc aatcgaaaag caaaaggaga
     1261 agagcatggt cgacgcggtc gttaaaactc ccgaattttt accttttact tctgtaggaa
     1321 tattccgttt tggtgctgat ataacacagt acaaaaacat actggaaact tttatgtatg
     1381 aacctcctga tgagtttgga acagaatatt atgaatctcc cgactcaaat ttactcattt
     1441 ccgtcgaaaa aaacaaaatt atttcgatat tttgttatca ggagctatat tttatgggcg
     1501 ttaatataat aggtctgaat tttgaagatt ttaaacagct tttccatcac cctaaatcat
     1561 acggtgttga taaatattat ttatcaaatg aatcttatcc gacatatgtc tatgagtttg
     1621 atgaaatagg agtacaagct tgggaagcaa aagggaaaat tgttactatc attgccgggg
     1681 gcaaagacaa ttattcgaca gaaccttact acgatgaata acat
//
LOCUS       DAWEWP010000226         1723 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWEWP010000226 DAWEWP010000000
VERSION     DAWEWP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.226"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WGN53_001976"
     CDS             complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WGN53_001976"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ0140910.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAMREAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1394..1567)
                     /locus_tag="WGN53_001977"
     CDS             complement(1394..1567)
                     /locus_tag="WGN53_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0140911.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
BASE COUNT          384 a          485 c          471 g          383 t
ORIGIN      
        1 caatctgcca aacagatcga acagagaaac tctgtccgtc aaaacatcat gcagccatcg
       61 ccttgaacac ttcaaccgca accgcaaccg tttcgtcaat cagctcgggc gtatgcgcgg
      121 cagaaacgaa acctgcttca taagcggacg ggccgaaggc aatgccgcgg tcgagcatac
      181 cgtggaagaa gcgtttgaaa gcgtcaatat tggaacgcgc catatcggca taatttcgcg
      241 gcgcgtgtgc ggcgaaatac agaccgaaca taccgcccac gctgtcggcg gtgaactcga
      301 tgcccgccgc atccgctgcc gtccgaaaac cttgaaccaa ctgttcggta cgcgccgtca
      361 ggttttcata aaacccttcg cggcggatga tttccagcgt tttcaaaccg gcggcgacgg
      421 caatcgggtt gcccgacaag gtgcctgcct gatacacgcc gcccagcggg gaaatacatt
      481 ccataatgtc tttgcgtccg ccgaacgcgg caagtggcat accgccgccg atgactttgc
      541 ccatcgtggt caggtcgggc gtgatgccgt gcagcgattg tgcgccgccg agcgcgacgc
      601 ggaaaccggt catcacttcg tcgtaaatca acactgcgcc gtgtttttcg gtcaattcgc
      661 gcagggcttt gacgaaggct tcagacggcc taaccagatt catattgccg acaaaaggct
      721 ccaaaatcac acaggcaatg tcattgccgc tttgagcaaa ggcttcttcg agttgggcga
      781 tgttgttgta ttcgaggact aaagtgtgtt tggtaaagtc ggcaggcaca ccggcggaag
      841 agggattacc gaaggtgagc agaccgctgc ctgctttcac caacaggctg tcggaatggc
      901 cgtggtagca gccttcaaat ttgatgattt tgtcacggcc ggtaaagccg cgtgccagac
      961 ggatggcggt catggtggct tcggtaccgg agctgacaag gcgcagccgt tcgacggacg
     1021 gcatgatttt ggcgatttct tcggcaatga cgatttcgcc ttcggtaggc gcgccgaacg
     1081 acaaaccgcc caaggcagct tcgcgcatgg cttcgatgac ttcggggtgc gcgtgtccga
     1141 caatcgcggg cccccacgaa ccgacatagt cggtgtagcg cgtgccgttt tcgtcccaaa
     1201 catacgcgcc ttcggctttt ttgatgaagc gcggcacgcc gccgacgctg ccgaatgcgc
     1261 ggacggggga attcacgccg ccggggatga tggctttggc gcggtcgaat aaaatttcgt
     1321 tacggttcat atttatcctc aaatgccgtc tgaacggcag gtttcgggct tagaagcaga
     1381 aagtcccatt ttatcatttt tcaggttgcg acaaggattt gcctgcttct ttgcggatga
     1441 cgccaaccgc atcccggatg acggaacgct cgtctttttc cactttatgt gtaaagcggt
     1501 agtcccggac aactccttcc cagtcgtaat ccacacacca ctcccaatgc cggcgttctg
     1561 atttcatata aatgaaattg gtcggcaaaa aattataaat cggcaggctg acttcatgat
     1621 aggcataaca accgaaaggg ttgcgcttcc cgaaacgtgc ctctacctcc gcccgagtcg
     1681 ttttgccttt aacaaccgtt tgtgcgattc cctcttccgt ctg
//
LOCUS       DAWEWP010000227         1722 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWEWP010000227 DAWEWP010000000
VERSION     DAWEWP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.227"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(96..758)
                     /locus_tag="WGN53_001978"
     CDS             complement(96..758)
                     /locus_tag="WGN53_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0140912.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPPKSMDEIRNQ
                     ASHGAAGLLKLGTGITPDHPDYARWRTEYLDEYDSRYAQDTTLFGGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLAPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(771..1598)
                     /locus_tag="WGN53_001979"
     CDS             complement(771..1598)
                     /locus_tag="WGN53_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ0140913.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNTPP
                     LDFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT          336 a          448 c          527 g          411 t
ORIGIN      
        1 cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgacgta ctggtttaga
       61 tttaatccac tatatgtttg tggggcggat atttttcagg gctgtatttt gtccagacat
      121 tcgagcagat cgagcggcgt gcggatgtgg aaatccgcct gccatgagcc ggtatcgtct
      181 tcgggagcga tgtagcccca ttcggcgagg acggttttca tgccggcgtt gcggcctgct
      241 tggatgtcgc gttcggcatc gccgacgtag agtgtgtgtt gcgggtcggc gtggatttgt
      301 ccgcacgcat acagcatggg tttgatgctg ggcttgggtt cgccgcaggt gtcgccgctg
      361 acgacgacgg cgggtgggat gatgaagccg agtttggggg cgagtttgtc ggtgaagcgc
      421 atgggtttgt tggtgatgat gccccatttg atgccgcgtc tgtcgagttc ggcgatgagt
      481 tcgtttacgc cgccgaagag ggtggtgtct tgggcgtagc ggctgtcgta ttcgtcaagg
      541 tattcggtgc gccatcgggc atagtcgggg tggtcggggg tgatgcctgt gccgagcttg
      601 agcagtcctg ctgcgccgtg gctggcttgg ttgcggattt cgtccatgct tttgggcggc
      661 agtccgtggc gggcgagcag ggtattgagt gcgccgccga ggtctagggc ggtgtcggca
      721 agcgtgccgt cgaggtcgaa gagtacggcg tgtatcatgt atgttccttt tcagacggta
      781 tttttgttct gtttcaaacc ctgctcgaaa tcttccagca tatccaattc aaagcggctg
      841 aagcccgctt tttcgcgcgc ttcgatgttc acatagccgc ggaagataaa catatcgtaa
      901 cgggcaatca gactgcggaa cagggtaatc ggttccaaac cgcgttcgcg gcagaggtgt
      961 tgataccaat ggttgccgat ggcgacatga cccacttcgt cgcggtaaat gatgtccaat
     1021 acgccgcagg tttccgaatc gccgcgctgc gccaccttcg cgcgtatccc cggcgtaacg
     1081 tccagcccgc gcgcttccaa cacgcgcggt accaacgcca tacgcaacag cgggtcgtag
     1141 gcggttttgt acgccatatc ccacaaatgg ttgtgcgcct cgaaactgcc gtaatcgaag
     1201 ccgaaagcgc gcaatctgtc gcgcatcagg cggaaatggt acacctcttc cttcgccact
     1261 ttcacccagt cgcggacaaa ctgaaacggc agcgtgcgga aacggtatgc cgcgtccaaa
     1321 gctagattga cggcgttgaa ttcgatatgc gcaatcgcgt gcagcatcgc cgcatagcct
     1381 tctgccgtgt tcattttgcg cggggtcagt tgtgacggcg cgaccaaaac cggcttgtcc
     1441 ggccgccctg cgcgggggaa atccagcggc ggcgtgtttg tttcagcacc gtccatgttc
     1501 tgaacggcgg caaacgcctc atccgtcagc cgtccttttt catctgggtc gcccgaaagc
     1561 agggcgcgtt ccagcaaagc ataaatatcg agtttcatct caagtccgcc gtgttcggaa
     1621 aacaaatatt atagcgtttt aaaaaaacaa gatgaggcat ataatctccg cgattcggca
     1681 ttccgcgccc aaaccgtcaa atatagtgga ttaacaaaaa cc
//
LOCUS       DAWEWP010000228         1646 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWEWP010000228 DAWEWP010000000
VERSION     DAWEWP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.228"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            295..1452
                     /locus_tag="WGN53_001980"
     CDS             295..1452
                     /locus_tag="WGN53_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0140914.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     QNIQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGATQWG
                     NRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMPVSVR
                     YDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQQTPKSQPTLVPAVVGKPGSDVYYAGL
                     NYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTGGYEE
                     GGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKGE
                     NTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          413 a          393 c          423 g          417 t
ORIGIN      
        1 tgccgcgtgt gtttttttat ggcgttttaa aaagccggga ctgcatccgg gcagcagcgc
       61 atcggcccgc acgaggtctg cgcttgaatt gtgttgtaga aacacaacgg tttttgaaaa
      121 aataagctat tgttttatat caaaatataa tcatttttaa aataaaggtt gcggcattta
      181 tcagatattt gttctgaaaa atggtttttt gcggaggggg gggataattg aagacgtatc
      241 gggtgtttgc ccgatgtttt taggttttta tcaaatttac aaaaggaagc cgatatgcga
      301 aaaaaactta ccgccctcgt attgtccgca ctgccgcttg cggccgttgc cgatgtcagc
      361 ctgtacggcg aaatcaaagc cggcgtggaa ggcaggaaca tccagctgca gttgaccgaa
      421 ccgctccaaa atattcaacc tcaggttact aagcgcaaaa gccgcatcag gacgaaaatc
      481 agcgatttcg gctcgtttat cggctttaag gggagcgagg atttgggcga agggctgaag
      541 gctgtttggc agcttgagca agacgtatcc gttgccggcg gcggcgcgac ccagtggggc
      601 aacagggaat cctttatcgg cttggcaggc gaattcggca cgctgcgcgc cggtcgcgtt
      661 gcgaatcagt ttgacgatgc cagccaagcc attgatcctt gggacagcaa taatgatgtg
      721 gcttcgcaat tgggtatttt caaacgccac gacgatatgc cggtttccgt acgctacgat
      781 tctccggaat tttccggttt cagcggcagc gtccaattcg ttccggccca aaacagcaaa
      841 tccgcctata caccggctca ttttgttcag cagactccta aaagtcagcc tactctcgtt
      901 ccggctgttg tcggcaagcc ggggtcggat gtgtattatg ccggtctgaa ttacaaaaat
      961 ggcggttttg ccgggaacta tgcctttaaa tacgcgaaac acgccaatgt gggccgtgat
     1021 gcttttgagt tgttcttgct cggcagcggg agtgatgaag ccaaaggtac cgatcccttg
     1081 aaaaaccatc aggtacaccg cctgacgggc ggctatgagg aaggcggctt gaatctcgcc
     1141 ttggcggctc agttggattt gtctgaaaat ggcgacaaaa ccaaaaacag tacgaccgaa
     1201 attgccgcca ctgcttccta ccgcttcggt aatgcagttc cacgcatcag ctatgcccat
     1261 ggtttcgact ttatcgaacg cggtaaaaaa ggcgaaaata ccagctacga tcaaatcatc
     1321 gccggcgttg attatgattt ttccaaacgc acttccgcca tcgtgtctgg cgcttggctg
     1381 aaacgcaata ccggcatcgg caactacact caaattaatg ccgcctccgt cggtttgcgc
     1441 cacaaattct aaatagcggg gcggtgaagc ggatagcttt gtttttgacg gcttgccttc
     1501 attctttgat tgcaatctga ctgccaatct gcttcagccc caaacaaaaa tccggatacg
     1561 gaagaaaaac ggcaataaag acagcaaata ccgtctgaaa gattttcaga cggtatttcg
     1621 cattttcggc ttggtttgca catata
//
LOCUS       DAWEWP010000229         1586 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWEWP010000229 DAWEWP010000000
VERSION     DAWEWP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.229"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            254..625
                     /locus_tag="WGN53_001981"
     CDS             254..625
                     /locus_tag="WGN53_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140915.1"
                     /translation="MNITLGRNHQINCDEKDLYKFIGYLANHPNDVNLVFEKNSVQGA
                     WGDEGRIQFFSSKAQNIFVPLGFKFTAGVGNIAYRLNCNELFEMLSQLDFVSGGKQNL
                     STIKANIPSQFHAEFDAGANM"
     gene            complement(689..1579)
                     /locus_tag="WGN53_001982"
     CDS             complement(689..1579)
                     /locus_tag="WGN53_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0140916.1"
                     /translation="MLSYCGVLSEEKFSRYYRYGVQNNKILQYIANRERNALNFIQAF
                     SEKLLKDSGIYPKFADFFAQPNKNTFESMKTAFTDLVIQNTPKNTEVEVRRIFTKIIN
                     PLAYKHNTFGTRKGSISNTPITLDELYYNRLNWRDKGKEKSLTRKEAQTLFADSANAA
                     NLNYLVNKATKFVKTLHKTSEVQRFDPTEANQAHHIFMASEFPDLASLPENLICLTPN
                     QHFNLAHPSNKTTVIDKHYQRICLMAKLDSIEQDNRANTGNYDYHEFIHVLNTGFNTD
                     QFDVSMSYETLKHRILMFDF"
BASE COUNT          488 a          238 c          314 g          546 t
ORIGIN      
        1 aattaggcgg tatttggggc gaaaacagcc gaaaacctgt gttggggttt cggctgtcag
       61 cgaaaaggtg aagagaggta ctttgcaaag gtctcaacct gaatatgtca accaaatcag
      121 cgacaagctc ggagaatttt ttggccggac gttgaaatag gagtttcaat tcttttaatt
      181 cagcaataga tactgataaa atgaaaatct tgaggaatat atcattaaaa ttaatttgtt
      241 aaggagataa ttaatgaata ttacattagg tcgaaatcat caaatcaatt gtgatgaaaa
      301 ggacctttac aagtttatcg gctatttggc aaatcatcct aatgatgtaa atttagtctt
      361 tgagaaaaat agtgtgcaag gtgcttgggg tgatgaggga cgtatacagt ttttctcaag
      421 taaagcacaa aatatttttg tgccactagg atttaaattt acggcaggcg taggtaatat
      481 tgcatatagg ctaaattgta atgagctttt tgaaatgcta tctcagttag attttgtttc
      541 tggtggaaaa caaaatcttt caacaataaa agcaaatatt ccgtctcaat ttcatgcaga
      601 atttgatgct ggtgcgaata tgtaaattct aatttaaaac acgaatccgc ttcatgagat
      661 tcgtgttttt tatttttaaa atatttattt aaaaatcaaa cattaaaata cgatgcttca
      721 atgtttcgta actcatagaa acatcaaact gatctgtatt aaatcctgta ttgagtacat
      781 gaataaattc atggtaatcg taatttcctg tattagcacg gttatcttgc tcaatactgt
      841 ccaatttcgc catcaggcaa atacgctggt aatgtttgtc aataacggtg gttttattgc
      901 ttgggtgtgc taaattgaaa tgctggtttg gtgtcaggca aatcagattt tcaggcagcg
      961 atgctaaatc aggaaattcg cttgccataa aaatatgatg tgcctgattg gcttctgtgg
     1021 gatcaaaacg ttgaacttcg ctggttttgt gtagcgtttt gacaaatttt gtggctttat
     1081 taactaaata attcaaattt gcggcatttg cactatcggc aaatagagtt tgagcttctt
     1141 tgcgagtgag cgatttttct ttgcctttat ctcgccagtt taggcgattg taataaagct
     1201 cgtctaatgt aatgggggta ttggaaatac tcccttttct tgtaccaaaa gtattatgtt
     1261 tataagccaa tggattaatg attttggtaa aaatacggcg aacttctact tcggtatttt
     1321 tgggtgtgtt ttggataaca agatcggtaa acgccgtttt catactttca aacgtatttt
     1381 tattaggctg ggcaaaaaaa tcggcaaatt ttggataaat accactgtct tttaataatt
     1441 tttcactaaa tgcttggatg aaatttaggg catttctttc acgattggca atatattgca
     1501 ggattttatt attctgtacg ccgtaccgat agtatctaga aaacttttct tcgcttaata
     1561 cgccacaata gctcagcatt ttaatc
//
LOCUS       DAWEWP010000230         1573 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWEWP010000230 DAWEWP010000000
VERSION     DAWEWP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.230"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            40..138
                     /locus_tag="WGN53_001983"
     CDS             40..138
                     /locus_tag="WGN53_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ0140917.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(209..1528)
                     /locus_tag="WGN53_001984"
                     /pseudo
     CDS             complement(209..1528)
                     /locus_tag="WGN53_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239425.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
BASE COUNT          288 a          429 c          507 g          349 t
ORIGIN      
        1 cttgccggat gctgcaaaca acgccggttt gcattcctga tggcggtggt tttcttagac
       61 gaacgcccga acacgcagga atggataggc ttggggctgg ttacggcggg cgtgttgacg
      121 ctggcactga aacggtaaag ccgcaagaaa taaatgaaat gccgtctaaa aaactgtttt
      181 cagacggcat tttcgtttct gtccatcctc agcactcgac cacgcgcacg gatacgggga
      241 cggctttttt ccggagcgtg gcggcgagtc cggcaagcga ggtttctttg taggttgcct
      301 tcatatcccg gccggtttgc agcatggttc ggatgacttc gtcgagcgag acttttttgt
      361 ccgtgccgtc ttccaaaagc gcgagcgtgc cgagtttgag ggctttttcg gcggcgatgc
      421 cgttgcgctc gatgcagggg atttgcacca gtccgccgac ggggtcgcaa gtcagcccca
      481 aatggtgttc catcgccatt tcggcggcgt tttccacttg tttgggcgtg ccgccgatga
      541 cttcggcgta tgcgcccgcc gccatcgaac acgctacgcc gacttcgccc tgacagccga
      601 catccgcacc ggaaatggag gcgttggtct tgtagaggat gccgattgcg cctgcggtga
      661 gcaggaagtt ttcgacgcgt tcctgtgtgg cgtgcggatt gaacttgcgg aaatagtgca
      721 atacggcggg aatgatgcct gccgcgccgt tggtcggtgc ggtaacgacg cgtccgccgg
      781 cggcgttttc ttcgttgacc gccatggcgt acaccatcgg ccagagctgg gtgttgacga
      841 tttcggtttc gcgcaggact ttgagcttgg cggcaagctg cggggcgcgg cggcggacgt
      901 tcaatcagcc gggcagttcg ccgtccgcac ccaagccgcg tttgatgcag ccttccataa
      961 cctcggcaac ggcagcggcg cggcggcgga tttcggcttc gccgcatccg gcaagcgcgg
     1021 cttcgtttgc caacacgact tcggagatgt cgagccggtt cagacggcat cgggcaagca
     1081 gttcggcgca actggtatag ggatagggaa cggctttttc cgtttccgcc tgccggtcaa
     1141 aatcttcttc ggtaacgaca aagccgccgc cgaccgaata ataaacctgt tcattcaata
     1201 ccgtgccgtc tgaagcatag gcggtaaaac gcaggctgtt ggggtgtttg ggcagcactt
     1261 gattgccgag tatgttcagg tcgcggtcgg ggatgaagcg gatttcttgc ccgttgagcc
     1321 ggaggatgtg ctgcgtgcgg atgcgttcga ggcgttcggg aatgccggca agcgggatgt
     1381 cgtgcggcag gctgccttcc aaaccgagca tcagcgcgtc aaatgtaccg tgtccgtatc
     1441 cggtcagtgc gagcgagccg taaatgtcga tgacgatgcg aacagcctgt gcatccaaac
     1501 ctgccgcaaa ggcggcggct gccttcatcg ggccgaccgt atgcgaactg gaaggcccga
     1561 taccgatttt gaa
//
LOCUS       DAWEWP010000231         1531 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWEWP010000231 DAWEWP010000000
VERSION     DAWEWP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.231"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..435
                     /locus_tag="WGN53_001985"
     CDS             <1..435
                     /locus_tag="WGN53_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0140918.1"
                     /translation="IIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVAKID
                     AIRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKEL
                     SDIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            465..1343
                     /locus_tag="WGN53_001986"
     CDS             465..1343
                     /locus_tag="WGN53_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0140919.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEALDLPEGSVILGVVLEVKKRF
                     MSAAQARRRVDSDYRP"
     gene            1347..>1531
                     /locus_tag="WGN53_001987"
     CDS             1347..>1531
                     /locus_tag="WGN53_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ0140920.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLSALSEWVGQLET"
BASE COUNT          404 a          395 c          411 g          321 t
ORIGIN      
        1 attatcgaaa acagggcgga ctattaccgc ctgcttttag gcgtaaccga acggcaggac
       61 tgggaaagct ggataatcta catcttagac ggcgtagccg acaccgccga ttggacggta
      121 gcgaaaatag atgcgatacg ccgcctgttt gagcagacac gtcaacacat acggacacac
      181 gcacaaggaa tctacacgca cgaactggta aatcttctgt ttgagcagcc atatacacgc
      241 attgccaacc tagaagcggc agggatagcc aaacggcaga cggcctctaa gtacctgaaa
      301 gagctttcag acataggtgt gctgcaagaa atcgccatcg gcagggacaa actattcatt
      361 catccgcgcc taatggaact attgcgggga gagggcaaca gctttacctc attccaatcc
      421 ctcgttaaag catagccaaa ataatcaata atccggaggt caatatggca agaaggtcaa
      481 aaacatttga agaagctgct gctgaggttg aggaacgttt cggtcatcgt ggcattaagt
      541 tggtcgagtt tgagggtaca gccaagccgt gtgtaatcaa ctgccctaaa catggaaacc
      601 aaacctgttc gaggtactcc aatatgttca taggaagtag ctggggttgc ccctcttgtg
      661 gtaatgagca agctgcaaaa gccggtatag cgacccttag gaagaatcac atagcgttag
      721 aaatgctgaa acaggctgta acaggtatga ccaagcaaga gcgcatcacg acgcaagcct
      781 acaatgagat gaccaaatcc gtggcaggtt caaacagcat agtccttaac gatgtccaag
      841 gcgatacgac catcaacaac catcatacgc atacgcacaa ccacagcgat gccgacggca
      901 aagcactgtc gatgaggctc acaccacgcc ccctgttgtc agaccgtcag gcggcggctt
      961 tcgcccgtac aggcaaactc acaggcagtt tcgacttgtt tgcttcggtg gtcgccccct
     1021 cgcagtacac gtttgccgtt gccatgcccg acacgtccat gtcgccggtt atcgaaaagg
     1081 gggatttgct ggtggtcgag ccgcgtatgc gccctgcgga cgaagacatc gtactgattg
     1141 aactgtccga caagcggctg gtcgtcgcgc accttgttat cgatattgcg ggcaggatgc
     1201 tgatttatca gacgggcagg ccgtctgaag ccctcgacct gcccgaaggc agcgtgattt
     1261 taggtgtggt gctggaggtc aaaaaacggt ttatgtccgc cgcacaggca agaaggcgtg
     1321 ttgattcgga ttaccgcccc tgatgtgtgg acggttggta cgatttccgc ttccaaaacg
     1381 tcgtgtacgc gcccgaccgc agcccggaaa tcagccgtat gctttcttcg attttggcag
     1441 gctacgcgtg ggataccgaa aacccgttcg tggcgaaatc cgaacaacgc ctgtcggcct
     1501 tgtcagaatg ggtcggtcag ttggaaaccg a
//
LOCUS       DAWEWP010000232         1528 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWEWP010000232 DAWEWP010000000
VERSION     DAWEWP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.232"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            180..509
                     /locus_tag="WGN53_001988"
     CDS             180..509
                     /locus_tag="WGN53_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0140921.1"
                     /translation="MGSLIIEDLQESFGKEAVKGKEITVHYTGWLEDGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            538..900
                     /locus_tag="WGN53_001989"
     CDS             538..900
                     /locus_tag="WGN53_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0140922.1"
                     /translation="MASSNVNKEIKDKKLSLWAKRQDGSVKWFCGQPVTRNDTAKDDT
                     VAADTDTAKKIDTKHLPSTCRDDSSTGCIETPRADFKHFQKISRYRVLPESRHMAEKL
                     RHSRAGGNLGLSARKLIG"
     gene            947..1234
                     /locus_tag="WGN53_001990"
     CDS             947..1234
                     /locus_tag="WGN53_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0140923.1"
                     /translation="MTGFNDAAGVASASDIKGKYVEKVEVKNGVVTAEMKSSGVNKEI
                     QGKKLSLWAKRQDGSVKWFCGQPVARNDKADTDKIDTKHLPSTCRDAASAD"
BASE COUNT          443 a          410 c          373 g          302 t
ORIGIN      
        1 aaatttaatc cactatattt ccatgaaaaa gcatggaacc tttacgaaaa aaataaaaca
       61 gtcaaacaaa cacagccgtt tcagctgcac cggagcagct gacgtttgag cggcatttga
      121 cttcgccgga cggtatgtta cccttgccgt tttaaccaac aagcaacgaa aggacaaata
      181 tgggcagcct gattattgaa gatttgcagg aaagcttcgg aaaagaagca gttaaaggca
      241 aagagattac cgtgcattac acaggttggc tggaagacgg caccaaattc gactccagcc
      301 tcgaccgccg ccagccgctg accatcacgc tcggcgtcgg acaagtcatc aaaggctggg
      361 acgaaggctt cggcggaatg aaggagggcg gcaaacgcaa gctgaccatc ccttcggaaa
      421 tgggctacgg cgcacacggc gccggcggcg tgattccccc gcacgccact ttgatatttg
      481 aagtcgagct gctgaaagtg tacgaataaa gctgcctggg caataccgtc tgacataatg
      541 gcttcaagca acgtaaacaa agaaatcaaa gacaaaaaac tctccctgtg ggccaagcgt
      601 caagacggtt cggtaaaatg gttctgcgga cagccggtta cgcgcaacga caccgccaaa
      661 gacgacaccg tcgccgccga caccgacacc gccaagaaga tcgacaccaa gcacctgccg
      721 tcaacctgcc gcgatgattc atctaccggt tgcatagaaa caccacgcgc cgacttcaaa
      781 cacttccaaa aaatcagccg ttaccgagta ttgcccgaat cacggcacat ggccgaaaaa
      841 cttcgtcatt cccgcgcagg cgggaatcta ggtctgtcgg cacggaaact tatcgggtaa
      901 aacggtttct tgagattttg cgttctagat tcccactttc gtgggaatga cgggatttaa
      961 tgatgccgcc ggcgtggcat ccgcctccga catcaaaggc aaatatgttg agaaagttga
     1021 agtcaaaaac ggcgtcgtta ccgccgaaat gaaatcaagc ggcgtaaaca aagaaatcca
     1081 aggcaaaaaa ctctccctgt gggccaagcg tcaagacggt tcggtaaaat ggttctgcgg
     1141 acagccggtt gcgcgcaacg acaaagccga caccgacaaa atcgacacca agcacctgcc
     1201 gtcaacctgc cgcgacgcag catctgccga ttaaggcaaa ttataccata aattttaaat
     1261 aaatcaagcg gtaaatgatt ttccacggcc actcggatta atccgggtgg cttccttttt
     1321 aaaggtttgc aaggcaagcg gggtcgtccg ttttggtgga aataatatat cgattgcgct
     1381 tcaaggctct gcgcgtacct cattgccgcc cgtttaaaca cggggtttat ctgacaggcg
     1441 cgtaatccgc cctctcattt accgaacaag cgaactggat tcccgcctac tcgggaatga
     1501 cgaagctatc cgtacggaaa cctgcacc
//
LOCUS       DAWEWP010000233         1516 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWEWP010000233 DAWEWP010000000
VERSION     DAWEWP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.233"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..128
                     /locus_tag="WGN53_001991"
     CDS             <1..128
                     /locus_tag="WGN53_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ0140924.1"
                     /translation="SKPEKRASDGIFACNSNADGQNQGASDTVIGHLVRVNLGLT"
     gene            complement(145..675)
                     /locus_tag="WGN53_001992"
     CDS             complement(145..675)
                     /locus_tag="WGN53_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ0140925.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(716..>1516)
                     /locus_tag="WGN53_001993"
     CDS             complement(716..>1516)
                     /locus_tag="WGN53_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0140926.1"
                     /translation="RTPNIKYIYQDNQGLSEARNTGIKNSNGKYIVFIDSDDFINCQV
                     LLDFLSKDDIDMPDVVFLNAVKYDKGSVSYFGEDYQPEKILNQSKVEVLKGLCRFRKF
                     PGSAWNKIIKRELIIKEKLFFEKGIYSEDVEWSMRLFNAATTFSYLDGCYYYYRQGRE
                     DSITGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIACD
                     ADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
BASE COUNT          419 a          371 c          283 g          443 t
ORIGIN      
        1 cgtctaaacc cgaaaaaagg gcttcagacg gcatttttgc ctgcaactca aacgcagacg
       61 gtcaaaatca aggcgcatcg gataccgtta tcgggcatct cgtccgcgtg aatttggggc
      121 tgacgtagga acgccgatgt gatttcacat cccgtactgt ttcgacagct tcacataatg
      181 cgcggcggaa tatttcaaaa aggctttttc ctcatcggtc agcacgcgca cttgtctgac
      241 cggcgaaccg acataaaggt agccgcccgc caaacgtttg cgcggcggaa cgaggctgcc
      301 cgcgccgatc atcacttcgt cctcaatcac ggcatcgtcc agaaccgtcg tccccatgcc
      361 gaccaggacg cggttgccga tacggcagcc gtgcagcatc actttgtgcc ccacggtaac
      421 gtcttcgccg ataaccagcg gcgacccttc gggtttggcg gcggttttgt gggaaacgtg
      481 caggacgctg ccgtcctgta tattgctgcg cgcgccgacg gtgatgctgt tcacatcgcc
      541 gcgcaacacg gcgcacggcc acacggaaac gtcttcggca agcgacactt cgccgatgac
      601 gacgcacgct tcgtctatca tacagctttc gtggatttcg ggcgtgcggt tttggaaagt
      661 tcggattgcg ttcatttttc ctccttcggt aagatgtata gtgttaaagg atttattaaa
      721 tattccccct gattgctttt aaaatcctgc ctgtaatatc aaccccgaat aatgtgatta
      781 tcgggaatat cagcttatat atcaatttat tggactttaa cagcataaac cttaaatgat
      841 acgcccttct ttttatatca gcatcacacg ctatattttt actcgtcatg ataaaaagca
      901 aaacgagata ttcgtaggaa agaaaagaat aaagataact tgatatatcc ctattaaatt
      961 ccatttccgc atttttctcc aaaatatata ataatgactt tatacttttt tccgaaacag
     1021 ttcccgtaat agaatcttct cttccctgcc gataatagta ataacaaccg tccaaataag
     1081 aaaaagttgt tgccgcatta aataatctca ttgaccattc gacatcttca gaataaattc
     1141 ccttttcaaa aaacagtttt tctttaataa tcaattctct ttttataatc ttattccacg
     1201 ccgaacccgg aaattttcta aatcggcata atcctttcaa aacttcgact ttggattgat
     1261 tgagtatttt ttcaggctga taatcttcgc caaaatatga aacacttccc ttatcatatt
     1321 taactgcatt taaaaaaacc acatccggca tatcaatatc atctttacta agaaaatcca
     1381 gcaagacctg acaattaata aaatcatccg aatcaataaa gactatatat tttccgtttg
     1441 aattttttat tccggtattt ctcgcctctg acaatccttg gttatcctga tatatatatt
     1501 tgatatttgg tgtccg
//
LOCUS       DAWEWP010000234         1486 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWEWP010000234 DAWEWP010000000
VERSION     DAWEWP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.234"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            27..1364
                     /locus_tag="WGN53_001994"
     CDS             27..1364
                     /locus_tag="WGN53_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0140927.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGQNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGIWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
BASE COUNT          276 a          355 c          402 g          453 t
ORIGIN      
        1 tatcttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtgct tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
      121 atacggcagg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
      181 tggtcgccct gcccgtccag cttgccgagt tttatatcgg gcgcacaggc ggtcaaaacg
      241 ccgtcgattc cttcagggct ctgcgtccga gtacgcaatg gctctgggtc gggcgcatgg
      301 gcgtttccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattaa
      361 attatgtcgt ccacagtttc acgggggcgg ttcataccgg cgcggacttt gaagccttgt
      421 tcggcgcgac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtctg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggc caggcgtttt ttgccttgag catcggcgtt tccgctatga
      721 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg ccggcagtgt
      901 ttatgaagat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcgttt tcgatgttgg aaacggtcat tgcctcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa cacacttggc ttatcggcac ggctattttc attgtcggca
     1081 tcccgtccgc gctgtctttc ggcatctggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactatgtt atttccgccg tcattatgcc tgtcggcgcg ttgggcgttg
     1201 ccgtatttac cgcctggatt caggacaagc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac cttgcgctac ctcgccccga
     1321 ttgccattat tattgttttc gtcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 tcaaagtata gtggattaac aaaaatcagg acaaggcgac gaagcc
//
LOCUS       DAWEWP010000235         1425 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWEWP010000235 DAWEWP010000000
VERSION     DAWEWP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.235"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..372
                     /locus_tag="WGN53_001995"
     CDS             <1..372
                     /locus_tag="WGN53_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ0140928.1"
                     /translation="RLRRLVLIFVNPLYIIATTVPAQQAFLDAAELMQWSIETEGLGL
                     NVISHKILGKDHAQVEFEAYFQDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQP
                     CICGSGKKFKACCGKYLQPVA"
     gene            375..556
                     /locus_tag="WGN53_001996"
                     /pseudo
     CDS             375..556
                     /locus_tag="WGN53_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
     gene            complement(590..1384)
                     /locus_tag="WGN53_001997"
     CDS             complement(590..1384)
                     /locus_tag="WGN53_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ0140929.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDEFWSGQHVFKRSVIAALEPNLFERWVRWIRENQ"
BASE COUNT          382 a          425 c          300 g          318 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat acatcatcgc tactaccgtt
       61 ccggcgcaac aggcattcct cgatgccgcc gaactgatgc aatggagtat agaaaccgaa
      121 gggctgggct tgaacgtcat ctcgcacaag atactcggca aagaccacgc ccaagtcgaa
      181 tttgaagcct acttccaaga cggacaacac cgatccgcac atcacgaact gtccggcttc
      241 gtcaacatcg gcggacaatg gtatttcatc gatccgactg ttccgcatcc tgcgatgaaa
      301 caaccctgca tttgcggatc gggcaaaaaa ttcaaagcct gctgcggcaa atatctgcaa
      361 cctgtcgcat aaaaatgccg tctgaacgtt cagacggcat tttcaacgcg caaaaaaaac
      421 cattcatacc aagggtaagt atgaatggtc aatacattgc gggaaaacgt cttacttgct
      481 gcactgccga aaagggagaa acgacagtgt taaccagcaa aagcattgta cctttattaa
      541 tgttaaaaaa cattaatttt gaaaatatat taacgaatat atctggaacc tactgatttt
      601 cccttatcca cctgacccag cgttcaaaca aattaggttc gagtgcagca ataaccgaac
      661 gtttgaacac atgctgtccg ctccagaatt catcgccttc caatgtcgtc agcctgccgc
      721 ccgcctcctc gaaaatcaac gcacccgcag cataatccca cagcttctgc ccgccgtgaa
      781 cataaacatc ataacgcccg caagccagat aacaccagtc cagcgtgctg ctgcccatac
      841 tccgtatcgt tccaaaaggc gcgagcgtac tcatgcggct ggaaagtttg cccgaacgca
      901 gatatttgat ttccacgccc gcaatcgcct cattgagttt tttatccacg agacgcaaag
      961 gcaggcgcgt cccgtttaaa aacgccccct gcccccgttc ggcataaaaa cattcgccgc
     1021 tgaccggatt gtagattacg cccaactcgg cacgcccgtt gcggacaaac gccaccgaca
     1081 ccgcaaaatg cggcagcccg ttgacaaaat tatttgtccc gtctatcgga tcgacaatcc
     1141 acagcccctt ttcccccgaa tattgttccc acaaagccga ctgttcctgc cgcgacattt
     1201 cctcgcccaa catcgggcaa tcgatcagaa gcggcaacgc ggcggcaaaa gccgtctgcg
     1261 cggcaatgtc cgcctcgctc aacatcgaac cgtcttcctt gcggtgggat ggcgtattca
     1321 aaaaacgcgg cataatttcg gtttgcgcga tatggcgcac gactttctgc aaacggtgta
     1381 acacttccta ctgtcctcat attttgaact tgcggcgcgc gaacg
//
LOCUS       DAWEWP010000236         1402 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWEWP010000236 DAWEWP010000000
VERSION     DAWEWP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.236"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>1402
                     /locus_tag="WGN53_001998"
     CDS             <1..>1402
                     /locus_tag="WGN53_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0140930.1"
                     /translation="PGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQTAKNVDWKQVQ
                     LAYDKWDYKQEGLTGAGAAIVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAAT
                     TTVSTAAAMQTAALASLYSQAAVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGAL
                     NQMGADIAQLNSKVRTELFSSTGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKD
                     NLGNAALGALVNSFQGEAASKIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGA
                     AVGEIVAESILGGRNPATLSDAEKHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVV
                     NNALNFDSTPTNAKKHQPQKPDKTALEKIIQSIMPAHAAGAMTNPQDKDAAIWISNIR
                     NGITGPIVITSYGVYAAGWTAPLIGTAGKAAISTCMANPSGCTVMVTQAAEAGAGIAT
                     GAVTVGNAWEAPVGALS"
BASE COUNT          398 a          339 c          382 g          283 t
ORIGIN      
        1 ctcccggcgg ctatatcgtc gacattccga aaggcaatct gaaaaccgaa atcgaaaagc
       61 tggccaaaca gcccgaatac gcctacctga aacagcttca gacggccaag aacgtcgatt
      121 ggaaacaggt gcagctggcc tacgacaaat gggactataa acaggaaggc ttgaccggag
      181 ccggtgcagc gattgtggtg attattgtaa ccgctctaac ttatggatac ggagcggctg
      241 cagcgggtag cgtaactgcc gcaggaagta gtacagccgc agctgcaaca acgacagcgg
      301 cagcaactac cactgtttct actgcagctg ccatgcaaac cgcagcttta gcctccttgt
      361 atagccaagc agctgtagcc atcatcaata ataaaggcga tgtaggcaaa gcattaaaag
      421 atctcggcac cagtgatacg gtcaagcaga ttgttacttc cgcactgacg gcgggtgcat
      481 taaaccagat gggagcagat attgcccaat tgaacagcaa ggtaagaacc gaactgttca
      541 gcagtacggg caatcaaacc attgccaacc ttggaggcag attggccacc aacctcagca
      601 atgcaggtat ctcagctggt atcaataccg ccgttaacgg cggcagcttg aaagacaact
      661 taggcaatgc cgcattggga gcattggtta atagcttcca aggggaagcc gccagcaaaa
      721 tcaaaacaac cttcagcgac gattatgttg ccaaacagtt cgcccacgct ttggcgggtt
      781 gtgttagcgg actggtacag ggaaaatgta aagatggggc aattggcgca gcagttgggg
      841 aaatcgtagc tgaatccata cttggcggca gaaaccctgc tacactcagc gatgcagaaa
      901 agcataaagt tatcagttac tcgaagatta ttgcgggcag cgtggcggca ctcaacggcg
      961 gcgatgtgaa tactgcggcg aatgcggctg aggtagctgt ggtgaataat gctttgaatt
     1021 ttgacagcac ccctaccaat gcgaaaaagc atcaaccgca gaagcccgac aaaaccgcac
     1081 tggaaaaaat catccaaagt attatgcctg cacatgcggc aggtgcgatg actaatccgc
     1141 aggataagga tgctgccatt tggataagca atatccgtaa tggcatcaca ggcccgattg
     1201 tgattaccag ctatggagtt tatgcagcag gttggacagc tccgctgatc ggtacagctg
     1261 gtaaagcagc tatcagcacc tgtatggcta atccttctgg ttgtactgtc atggttacgc
     1321 aggctgctga agcgggcgcg ggaatcgcca cgggtgcggt aacggtaggc aacgcttggg
     1381 aagctccagt aggagcgttg tc
//
LOCUS       DAWEWP010000237         1380 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWEWP010000237 DAWEWP010000000
VERSION     DAWEWP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.237"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(73..996)
                     /locus_tag="WGN53_001999"
     CDS             complement(73..996)
                     /locus_tag="WGN53_001999"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ0140931.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPAFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          370 a          328 c          341 g          341 t
ORIGIN      
        1 ttttggtttt ctgtttttgt aggaatgatg aaaatttaag ttttaggaat ttaccggaaa
       61 aaacagaaag cgttatccac aatttctgat gttcagctcg tgaaatgcgt cgggcaaatc
      121 atcgctgtcg gcaaattcca cccggtcgta agccgtttcg tctgccaaaa ccgcgcgcaa
      181 gagtgcgttg ttgatggcgt gtcccgattt gtagccttca aacgcgccga caatcggatg
      241 tccgacgata tacaaatcac cgatggcatc aaggattttg tggcgcacaa actcatcggg
      301 atagcgcaag ccttcaggat tcaggacatc cgtgtcgtca atcacgatgg cgttgttcaa
      361 attgccgccc aaacccagat tgtgggcgcg catcatttcc acttcgtgca taaagccgaa
      421 ggtgcgcgcg cgcgcgattt cgtcgatgta ggatttgccg gcgaaatcga tttcaaaagt
      481 gggcgagctg cggttgaaag ccggatggtc gaattcgatg gtcagcgtta ccttaaagcc
      541 gtcatacggc gtaaagcgca cccatttgcc cgcttctttg atttcgacag gcttgaggat
      601 tttcaaaaaa cgcttttgcg ccttttgatc gaccacgccc gcatcttgca aaaggtaaat
      661 aaacggcagg ctggagccgt ccataatcgg gatttcgggt gcgttcagct caatcagcgc
      721 gttgtcgata ccgtaggcgg acagcgcgga cataatgtgt tcgatcgtgc cgacgcgcac
      781 gcctttgtcg gtaacgatgg tggaggaaag gcgggtatcg ttgatcaaat aaggggtcag
      841 cttgatttgt tcgcccatct cgccgtccaa atcggtacgg cggaaggaaa tcccgctgtt
      901 ttcaggcgcg gggtgcaggg tcagtgcgac ccgttcgccg gaatgcaggc cgactccggt
      961 aacgctgatg gatttcgcca aagttctttg cagcataaac cgcttcctta tcaatggggt
     1021 aagttgggga atgatacgat aaaaccggaa aaacaagcta tgtttttcca tagtatttgc
     1081 caatgtatcc gttttcaata cgtaagctgc ataaaaatgt atagtggatt aacaaaaatc
     1141 aagacaaggc gacgaacccg cctccctcct gaaaaacgca aaaaatgccg tccgaaaacc
     1201 tttcggacgg cattttcgcg tgaaccgtca ttcccacaag gacataaaac caaaacagaa
     1261 aaccaaaaac agaaacctaa aattcgtcat tcccacgaaa gtgggaatct agttcgttga
     1321 gtttcagtta tttagaataa attttgaaac ttaaatcccg tcattcccac gaacctacat
//
LOCUS       DAWEWP010000238         1346 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWEWP010000238 DAWEWP010000000
VERSION     DAWEWP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.238"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            57..782
                     /locus_tag="WGN53_002000"
     CDS             57..782
                     /locus_tag="WGN53_002000"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ0140932.1"
                     /translation="MPRFHLPENLSIGQTVNLPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAGAEILHEETADAESPLNITLIQSISSGERMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          373 a          408 c          309 g          256 t
ORIGIN      
        1 gtccgtttcc atcacgccgc tgcgacagat tataaccgtc cgaaccgcca aaaactatgc
       61 cccgattcca cctgcccgaa aacctttcca tcggacaaac cgtcaacctg cccgacaaca
      121 tcgtccgcca cctcaacgtc ctgcgcgtcc gccccaacga aaacatcacc ctcttcgacg
      181 gcaaaggcaa ggcacacgcc gcacggctga ccgttttgga aaaacgccgc gccggggcgg
      241 aaatcctgca cgaagaaacc gccgatgccg agtccccgct gaacatcacg ctcatccaat
      301 ccatatcttc cggcgaacgt atggacttta ccctgcaaaa aagcgtcgaa ctcggcgtaa
      361 ccgccataca gcccgtcatc agcgaacgct gcatcgtccg cctcgacggg gaacgcgccg
      421 ccaaacgcct cgcacgctgg caggaaatcg tcatctccgc gtgcgaacaa agcggcagga
      481 acaccgttcc ccccgtactg cccatcatcg gctaccgtga agcactcgac aaaatgccgt
      541 ctgaaagcac caagctgatt atgagcatca accgcgcccg caaactcggc gacatccgcc
      601 acccgtctgg cgcaatcgtc tttatggttg ggcccgaagg cggctggaca gaacaggaag
      661 aacaacaggc atttgaagct ggctttcagg cggttacact cggcaaacgg attttacgca
      721 cagaaaccgc cccactcgcc gccctcgccg ccatgcagac gctttggggc gatttcgcat
      781 aaacagaaat gccgtctgaa acccgttcag acggcatttt gcagccgatt aagatagtag
      841 gttcaaataa gatttcccgt gtcgtcattc ccgcgaaagc gggaatctag aaacgaaaaa
      901 ctacagggat ttatccgaaa caacaaccct ctccgccgtc attcccgcaa aagcgggaat
      961 ctagaaacga aaaactacag ggatttatcc gaaacaacaa accctctccg ccatcattcc
     1021 cgcaaaagcg ggaatctaga aatttaatgt tgcggtgatt tatcggaaat gactgaaact
     1081 caacgaaccg gattcccact ttcgcgggaa tgacgaagtg gaagttaccc gaaacccaaa
     1141 acaagcgaaa ccgaacggac tggattcccg cctgcgcggg aatgacgaga ttttaggttt
     1201 ctatttttgg ttttctgttc tcgcgggaat aacggaattt taagttttag gaatttgtcg
     1261 gaaaaacaga aatcccccgc cgtcattccc gcaaaagcgg gaatctagaa atttaacgtt
     1321 gcggtgattt atcggaaatg actgaa
//
LOCUS       DAWEWP010000239         1327 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWEWP010000239 DAWEWP010000000
VERSION     DAWEWP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.239"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1..1327
                     /locus_tag="WGN53_002001"
                     /pseudo
     CDS             1..1327
                     /locus_tag="WGN53_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
BASE COUNT          433 a          265 c          310 g          319 t
ORIGIN      
        1 gggataatag aaagtagtta atatctgctt tagataaagg atttgatgga tcttttaaag
       61 agaagcattt tactttttta caatctgtga tgatggatct aacaaagtta ggtgttgaat
      121 atacaataga tggttggcaa aaaattggag gttggggtaa tgggataatc aatgatttat
      181 ataaaagtgt tgtaaaaaga gagtggactg gaatatttga gatcgttaat aataacatca
      241 agcaaggaaa tgaagctttt aaaaatgaaa tcaatagctt ggttcatgat atgaaagctg
      301 ctggcaagga atttggagat gacttaaata cacagtggaa taatctcact caggctgccg
      361 aaataatcta taatgacata gtagacaata ctagtcaagg aatagaaaaa ggtgtcaaag
      421 ccattaaaga attgtctgaa aaaatgaaaa atgctgcttc cgatttggct gacggttcag
      481 cagagaaagc taaacaagta gtggaagatt tggctcaagc cgccaaagaa gcatacgaaa
      541 atgccaaatc cacagccgag aaggctgctc aagcagctcg agaatttttt aagggcttgc
      601 ccagttttaa agatctggcc gaaaaattta gagatctgtt cccaaatccg gaaggctgga
      661 tcgatgatgg tcaccaatgt ttcgctcctt gggttaaaga aactaaaaaa cgcaatggca
      721 aatatcatgt ctacgacccc cttgccctag atttggacgg cgacggcata gaaaccgttg
      781 ccaccaaagg cttttcaggc agcctatttg accacaaccg cgatggcatc cgcaccgcca
      841 ccggttgggt ttctgccgat gacggtctgc ttgtgcgcga tttgaacggc aacggcatca
      901 tcgacaacgg tgcggaactc ttcggcgaca acaccaaact ggcagacggc tcttttgcca
      961 aacacggcta cgcggctttg gccgaattgg attcaaacgg cgacaacatc atcaacgcgg
     1021 cagacgccgc attccaaacc ctgcgtgtat ggcaggatct caaccaggac ggcatttccc
     1081 aagctaatga attgcgtacc cttgaagaat tgggtattca atctttggat ctcgcctata
     1141 aagatgtaaa taaaaatctc ggtaacggta acactttggc tcagcaaggt agctatacca
     1201 aaacaaacgg tacaaccgca aaaatggggg atttactttt agcagccgac aacctgcaca
     1261 gccgttttaa agataaagtg gaactcactg ccgaacaggc aaaagccgcc aatcttgcgg
     1321 gcattgg
//
LOCUS       DAWEWP010000240         1290 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWEWP010000240 DAWEWP010000000
VERSION     DAWEWP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.240"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            166..1278
                     /gene="nadA"
                     /locus_tag="WGN53_002002"
     CDS             166..1278
                     /gene="nadA"
                     /locus_tag="WGN53_002002"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ0140933.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEADTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYICRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
BASE COUNT          299 a          333 c          387 g          271 t
ORIGIN      
        1 accaaagcgg tttgtcggaa cgaagcgggt cggttttacg gtatcgcaaa cctgttttca
       61 gacgaccttg cttattatac tcaatttgag cataatataa aaatatttca aaatataaac
      121 ggcaaatctt tatgccgtct gaaaaaccag ccataaggaa gtctcatgca aaccgccgcc
      181 cgccgctcgt tcgattacga tatgcccctc attcaaacgc cgacttccgc ctgccaaatc
      241 cgtcaggcgt gggcgaaggt tgccgacacg cccgaccgcg aaacggcagg ccgtctgaaa
      301 gacgaaatca aggctttgct gaaggagaaa aacgcggtct tggtggcgca ttattatgtt
      361 gatccgctga ttcaggattt ggctttggaa acgggcggat gcgtgggcga ttcgctggaa
      421 atggcgcgct ttggtgcgga acacgaagcc gatacattag tggtggcggg cgtgcgtttc
      481 atgggcgaga gcgcgaaaat cctctgccct gaaaaaacag tgctgatgcc tgatttggag
      541 gcggaatgtt ctttggattt gggttgcccg gaagaggcgt tttcggcgtt ttgcgaccaa
      601 caccccgacc gcacggtggt ggtgtacgcc aacacttccg ccgccgtgaa agcgcgtgcc
      661 gactgggtgg taacgtcttc agtggcgttg gaaatcgtgt cgtatctgaa atcgcgcggc
      721 gagaagctga tttggggacc cgaccgccac ctcggcgact acatctgccg cgaaacgggc
      781 gcggatatgc tgttgtggca gggttcgtgc atcgtccaca acgaatttaa agggcaggag
      841 ctggcggcgt tgaaagcgga acatcccgac gcggtggtgc tggttcatcc tgaatcgccg
      901 caaagcgtca tcgaactggg cgacgtggtc ggctcgacca gcaaactgct caaggccgcc
      961 gtatcgcgtc ctgaaaaaaa attcatcgtg gcgaccgatt tgggcatcct gcacgaaatg
     1021 caaaagcagg cgcccgacaa agaatttatc gccgcgccga cggcgggcaa cggtggaagc
     1081 tgtaaaagct gcgcgttctg cccgtggatg gcgatgaatt cgctgggcgg catcaaatac
     1141 gccctgacaa gcggacgtaa cgaaatcctg ttggacagga agctgggcga agccgccaaa
     1201 ctgcctttgc agcgtatgct cgacttcgcg gcaggactca aacgcgggga tgtgttcaac
     1261 ggcatgggtc ccgcctgatt tgccgtccat
//
LOCUS       DAWEWP010000241         1272 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWEWP010000241 DAWEWP010000000
VERSION     DAWEWP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.241"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..890
                     /locus_tag="WGN53_002003"
     CDS             <1..890
                     /locus_tag="WGN53_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0140934.1"
                     /translation="TAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVSEAKA
                     NLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNPALKNLSP
                     AQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
BASE COUNT          350 a          373 c          325 g          224 t
ORIGIN      
        1 ccacggcgca aaaagaagcc gcacccgcac aacagcaggg cggtatccgc ttttcagacg
       61 gcattgccgc cgttgccgac aacgaagtca tcacgcgccg ccggcttgcc gaagccgttt
      121 ccgaagccaa agccaacctg cccaaagacg cgcagataag cgaatccgag ctgtcccgac
      181 aggtgctgat gcagcttgtc aaccaatccc tgattgtaca ggcgggcaaa cgccgcaaca
      241 ttcaagcaag cgaagcggaa atcgatgccg tcgtcgcaaa aaatcccgcc ctcaaaaacc
      301 tcagccccgc ccaacgccgc gattttgccg acaacatcat tgccgaaaaa gtccgccagc
      361 aggcagtgat gcagaacagc cgcgtgagcg aagctgaaat cgatgccttc ctcgagcagg
      421 cgcaaaaaca aggcatcacc ctgcccgaag gcgcaccgtt gcgccaatac cgcgcccaac
      481 acatcctgat taaagctgac agcgaaaacg ccgccgtcgg cgcggaaagc accatccgca
      541 aaatctacgg agaggcccgc agcggcacag acttttccag cctggcgcgc caatattcgc
      601 aagacgcgag cgcgggcaac ggcggagatt tgggctggtt tgccgacggc gtgatggttc
      661 ccgcctttga agaagccgtc cacgcgctca aacccggaca ggtcggcgcg cccgtccgca
      721 cccaattcgg ctggcatatc atcaaattga acgaagtgcg cgatgccggc acacctcagg
      781 aacgtatccg caattccgtg cggcaataca tcttccaaca aaaagccgaa caggcaaccg
      841 tcaacctgtt gcgtgacctg cattccggcg cgtatgtcga catccgctaa ggcggtttga
      901 agcaaaaagc cataccgatc ggcaaaaatc cgggcggtat ggctttttgg atttcgagtt
      961 acttttacac cgtcattcat cattcccgcg aaagcgggaa tctagaaacg aaaagtaaca
     1021 ggaatttatc gggaatggct ggaatttaaa ggtctggatt cccgctttcg cgggaatgac
     1081 gggattttgg gttgtggtaa tttatcggaa aaacaaaaaa acctatgccg tcattcccga
     1141 gcaggcggga atccggttat ttaaaactgc agaaatttat ccgaagcaac aacaatcttt
     1201 ccatcgtcat tcccgcgtag gcgggaatct agcaacgaaa agtaacagga atttatcggg
     1261 aaaacagaaa cc
//
LOCUS       DAWEWP010000242         1254 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWEWP010000242 DAWEWP010000000
VERSION     DAWEWP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.242"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            237..1190
                     /locus_tag="WGN53_002004"
     CDS             237..1190
                     /locus_tag="WGN53_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0140935.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          200 a          273 c          432 g          349 t
ORIGIN      
        1 caaaccggat gattcgggct aaataattcc tgattctttg gatatagcca acggtatgcc
       61 tgccgatgcc gtcagaaagg gcttgatggg cgtttgcggt tcgggttaat atttgtgctg
      121 tatctgttaa ttccggttag taaaatgggg cggggacgat gagtggccgt cggtcggttt
      181 tgcacgggcg gtaataatgt ccggcgcaag gtgcgccgtt tcgggagggg tgtgtgatgg
      241 gttttctgat tggtttgagc cgcaacctga cgcggtttac ggctttggtc attttgcttg
      301 cgtcggcggt tgcgtttatc ggaccggctt cgtttgcgtg ggttaagggc gatgttcagg
      361 tcttggtgct gggcatcatt atgctgggta tggggatgac tttgggcagg gaggactacc
      421 ggattttggc gcagcgtcct ttggatgtgt ttatcggtgc ggcggcgcaa tataccgtga
      481 tgccgttgct cgccatcggt attgccaagc tgttcgacct ttcgccgggg ctgactttgg
      541 ggctggtgtt ggtggggacg tgtccgggcg gcgtgtcgtc caacattatg agtttccttg
      601 ccaaaggcga tgtggcgttt tcagtcggta tgacgacggt gtctacggtg ctggctccgg
      661 ccgttacgcc tttgtggatg acctgcctgg tcggtcagac ggtggatatg gacggttggg
      721 ggatgttccg gtttatgctg ctggtcacgc ttttgccggt ggtgctgggt tcggcggcaa
      781 atatcctgct gcacggaaaa cattggtttg aaaatgtccg tgccgttatg ccgggcgtgg
      841 cggtattggc atttgcctgc attgtcggcg gcgtggcttc ggttcacgga caccgttttg
      901 ccgaatcggc gttggtaatg gtgttggcga ttgcggtaca caatatcgcc ggctatgtgt
      961 caggctatta cgccgccgcg ctgtttggta tggatacggc aaaaaaacgc accttggcaa
     1021 tcgaagtcgg cgtgcagaat gccggtttgg caacaggatt gagcgcgaaa tttttcccag
     1081 gcaaccccga atcggcggtt gcggcggcgg tcgcctgcgt gtggcattcg gtttcgggga
     1141 cggtattggg caacttgttc gctctgtggg acaagcgtcg ggacgcttag tcgggcgtgt
     1201 ccgaaccgtc ccacgatata tagtggatta actttaaacc ggtacggcgt tgcc
//
LOCUS       DAWEWP010000243         1234 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWEWP010000243 DAWEWP010000000
VERSION     DAWEWP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.243"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(172..609)
                     /gene="rnhA"
                     /locus_tag="WGN53_002005"
     CDS             complement(172..609)
                     /gene="rnhA"
                     /locus_tag="WGN53_002005"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ0140936.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDTLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            722..1111
                     /locus_tag="WGN53_002006"
                     /pseudo
     CDS             722..1111
                     /locus_tag="WGN53_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
BASE COUNT          232 a          303 c          373 g          326 t
ORIGIN      
        1 ctggattccc gcctgcgcgg gaatgacggt ggagcggttt cagttgtttc taaataaatt
       61 tttgcagcgt tgggttttta gattcccgcc tgcgcgggaa tgacggtgga ggacgatgcc
      121 gtctgaatcc cattagcggt ttcagacggc attttcttgt cggtgcgaca gtcaggaaaa
      181 ctgcgctgcg ccacggtttg ccaaatcgtc ggcgcgttcg ttttccgcgt gtcccgcgtg
      241 tcctttaacc caagtccaac tgacttggtg ctgcccgacc agagtgtcga gttctttcca
      301 taaatcgtcg tttttgacgg gctgtttggc ggcggttttc cagccgttgc gcttccaacc
      361 gtgtatccag ttttccatgc cgtttttgac gtattgcgag tcggtgcaga tgatgacggt
      421 gcagcggcgt ttgagcgatt tcagtccttc gatgacggcg gtcagctcca tgcggttgtt
      481 ggtggtttgc gcttcgccgc cgaaaagttc tttttcgtgg ctgccgtagc gcatcaacac
      541 gccccagccg cccgcgccgg gattgccttt gcacgcgccg tcggtgtaaa ggtaaacggg
      601 tgtgtccata ttgtgccttt gtgtggtgtc cggcgtgatt ataagcggtt tgcgcgccgt
      661 gccgtctgaa agattgcggc ggattgaaat aggcatataa tgtgcaactt tatttttgaa
      721 tatgaaagag cggatagtgg gtcaaagcgg cgagttgttt tgtttcgggc aaatgcccgt
      781 atggaaggcg gaaaacctgc cggaagtttt gttgtcgggc tattcgtccg aggaagggga
      841 atgggtctgc ctgaatgtgt tgcagggcga tgtcgaagtc cgcacgcagg acggggcggc
      901 ggaggtttgg tcggcagaaa gcggcgattg cgtgtttgcg ccgcagcagg tgttttcggt
      961 caaactgaaa acggacgatg ccgaaatccg tttgtcgctg tattgcgcgg cggcagacta
     1021 ttttcacaaa aaatacggga tgagtgccac gcattctgcg gtcgcggcgg cacgggatac
     1081 cgtaccggcg ggcagggcgt tggatatggg ttgagacctt tgcaaaattc ctttccctcc
     1141 cgacagccga aacccaaaca caggttttca gctgttttcg ccccaaatac cgcctaattt
     1201 tacccaaata cccccttaat cctgcccaga tacc
//
LOCUS       DAWEWP010000244         1233 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWEWP010000244 DAWEWP010000000
VERSION     DAWEWP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.244"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            41..373
                     /locus_tag="WGN53_002007"
     CDS             41..373
                     /locus_tag="WGN53_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ0140937.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            705..1214
                     /locus_tag="WGN53_002008"
     CDS             705..1214
                     /locus_tag="WGN53_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ0140938.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          328 a          233 c          295 g          377 t
ORIGIN      
        1 gccttgtttt gacggaaaac catcatataa aggaacactt atgcagattt tatcttttca
       61 gccggacatt gcggaacgta tgctggaagg tacggaaggc gagtcggtca acgaaaacgc
      121 acaattcgtc cgtacggaca acggctattg gattgcgtgg catgaaggcg tagcggcact
      181 gcttgcgccc gatacgccgc cgggcattcc ctgtttttgg gtggaaggcg cggaaagcct
      241 tgaagagttg tgcgccatgg tggaacgcgg cgagtttgac gaagtggaag agtttgacgg
      301 cgatgacgac gaatggctcg aaacggcaca gggttgcggg caccacggcg acgcttgcgc
      361 ctgcggacat taaaggcatt gcaggcttgc cgcaaggggg gcaaggcttt gccgtttttt
      421 aaataatcgg tggattgcat ctgtttgcaa tgggctgaac gcttcccttt gtattttatt
      481 gaataaaaaa acttatcttg gtattataat taaggcagca tcaattattt tgggatggca
      541 ataaacgcaa agcattgatt tgcgccgatt gcagacttat tatagcaggt tgcggcgcgg
      601 acttaatggg aattgtttat agagcatgat ggtctgaaac atcattaatt aaaaatacca
      661 ttaatccgat ttatatttat ttcaatttca atggaaaaac atcaatgaca atgattttaa
      721 gcattttaag cctgtttttt atcatcagac tgttattttt agccgtctct attaaacatg
      781 aaaaagcctt gattgccaaa ggggcaaaac aatacggaaa aaccaattcc acgctgcttg
      841 cggcagttca tacgctttat tatttggcgt gttttgtttg ggtatggctt tctgacactg
      901 cttttaatgg catatccttg attggtacgc tgacggtgat ggcttcgttt gtgatattgt
      961 cattgattat taagcagttg ggggagattt ggacggttaa aatctatatt ttaccaaatc
     1021 atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca ccccaattat tttttaaaca
     1081 tcatacccga actgattggc atcgccttat tatgtcaagc gtggtatgtt ttattgattg
     1141 gcctgcccat ttatttgctg gtcttattta agcgtatccg acaagaagaa caggcgatgg
     1201 caacactttt ttaacccgtt tcatcaatta tag
//
LOCUS       DAWEWP010000245         1202 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWEWP010000245 DAWEWP010000000
VERSION     DAWEWP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.245"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            388..543
                     /locus_tag="WGN53_002009"
     CDS             388..543
                     /locus_tag="WGN53_002009"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ0140939.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(633..1082)
                     /locus_tag="WGN53_002010"
     CDS             complement(633..1082)
                     /locus_tag="WGN53_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0140940.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYIEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT          400 a          262 c          178 g          362 t
ORIGIN      
        1 ttatgtctaa aggtgcaaac atgccttaaa ttaccatagt ggattaaatt taaatcagga
       61 caaggcgagg caacgctgta ctggtttaaa tttaatccac tatatattgc ataggcaaaa
      121 tacaagccta taacgaattg gaaacaaaat gccgtctgaa aacatcttca gacggcatta
      181 taaaatctgt tcaccttttc agatgagtaa tgtacaccct tatacaattt ttgctactat
      241 gccccataaa tccacggcta aagatatcct tattatgtcc tatgatttat cgaaacgact
      301 tgtaatcggc ttagcatcaa gtgccctatt cgacttatcc gaatcggata atatatttag
      361 aatggaaggg gcagaaacct ataggcaata tcagagagaa aaacaaaacc atcccctaaa
      421 aaaggcgttg tctttccatt tattaaaaaa cttctgtcaa tcaatgaaat aaacccaaac
      481 gacccaacga ttgggtttat tcttttatcc agaaacaatc cagatacaga ttacgagtca
      541 taactatagg cttaatatta cacgattttc attccatcaa ggcggaaaac cgcacaaata
      601 ctgaaacact atcgatcgat ttgtaaacaa gcctacttaa gtaacttgca gtccttatca
      661 tttcctttaa aataatccag cccgtcacta cacgaactgg cggacttctt gcaaataaag
      721 gttactagat tttcattcat cttaataata aaaggatttt tatctttatc tatggctacc
      781 gccttcaaca tgaatttact gtctaaggcc ccgcgcgcga ttccattcaa acggatacaa
      841 aagccttctg cctctttaat cggcaaactt ggccatttgg tagatgtttg tttaaatctc
      901 ccattctgca gataaaactt ttccataaaa tgtgcatttt ctaacaaggc tgcccgcact
      961 gcatttatct ttgctttctc aatataattg cgatagctcg gataaacaat taaagcaagt
     1021 acagacaata tcaagaccac tgatattaat tcaaccagcg taaacccccg attatcagtc
     1081 attactttac ttccaataag aacagattat tcaacatatt tctttgaaca gacttactat
     1141 cccattcaac agtatgcata tttcccactc tattttttag cggccggtat agccggtttg
     1201 gc
//
LOCUS       DAWEWP010000246         1151 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWEWP010000246 DAWEWP010000000
VERSION     DAWEWP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.246"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..429
                     /locus_tag="WGN53_002011"
     CDS             <1..429
                     /locus_tag="WGN53_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0140941.1"
                     /translation="AIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQ
                     PVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTV
                     DVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            436..945
                     /locus_tag="WGN53_002012"
     CDS             436..945
                     /locus_tag="WGN53_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140942.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
BASE COUNT          400 a          199 c          197 g          355 t
ORIGIN      
        1 gctataccaa cccagacagt taaagaactt gatggcttac tacaagaatc aaaaaatata
       61 ggtgctgtaa atacacgaat taatatagcg aatagtacta ctcgatatac accaatgaga
      121 caaacgggac aaccggtatc tgctggcttt gagcatgttc ttgaggggca cttccatagg
      181 cctattgcga ataaccgttc agtttttacc atctccccaa atgaattgaa ggttatactt
      241 caaagtaata aagtagtttc ttctcccgta tcgatgactc ctgatggcca atatatgcgg
      301 actgtcgatg taggaaaagt tattggtact acttctatta aagaaggtgg acaacccaca
      361 actacaatta aagtatttac agataagtca ggaaatttga ttactacata cccagtaaaa
      421 ggaaactaac taaatatgag taactttgaa aaaaaatata ttttagaatt aaatgatgct
      481 ttaagccatt taaatcataa ctctacctca tttgatttat tgaaagtttt gatttcatgg
      541 ttatcaaacg atattgtcat tgataaattt aaaattttag gttatgactt tagtaaatat
      601 atcgaaatga atcccgatga ctatccggtt gaaaaatcta tattgaatag agaggaaatt
      661 atttatctca aaaacaatat ttatcgtaaa atatcatcag gaaattttaa atttcaatac
      721 tttgtacaat atattagaga tattttagaa tatttattta ttgaacatat tgaaagagtc
      781 tgtccttact gcgaatgggg tgaaatgcaa aaattagaag aacaaaatac gcatgaaacg
      841 gtgtatctct gtactcaatg tggatgtgct ttttataacg ataattcaca atttttatta
      901 aaaacccctt taaccattcc aatgaaacgt gatgaattta aataaacaag ccgtagcctg
      961 catgaaccct aaaatccacg tgtagcgtgt gtgcgccagc acgcatgcgt tccatgattt
     1021 acggctcaat gccgtctgaa aagctcacaa tttttcagac ggcatttgtt atgcaagtaa
     1081 atattcagat tccctgtatg ctgtacagac gcgggagtgt taagcccccc ttgtttgaag
     1141 ctccgcggct c
//
LOCUS       DAWEWP010000247         1144 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWEWP010000247 DAWEWP010000000
VERSION     DAWEWP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.247"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            32..>1144
                     /gene="tuf"
                     /locus_tag="WGN53_002013"
     CDS             32..>1144
                     /gene="tuf"
                     /locus_tag="WGN53_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0140943.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMV
                     MPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGV"
BASE COUNT          296 a          328 c          300 g          220 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgcccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gccaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatt gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 aaaaatcttc gaactggctg ccgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaaccg ttcctgctgc ctatcgaaga cgtgttctcc atttccggcc gcggtacagt
      661 agtaaccggc cgtgtagagc gcggtatcat ccacgttggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa acccaaaaaa ccacctgtac cggcgttgaa atgttccgca aactgctgga
      781 cgaaggtcag gcgggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agagcgtggt caggtattgg ccaaaccggg tactatcact cctcacacca agttcaaagc
      901 agaagtgtac gtactgagca aagaagaggg cggccgccat accccgttct tcgccaacta
      961 ccgtccccaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaatgccgg gtgagaacgt aaccattacc gtagaactga ttgcgcctat
     1081 cgctatggaa gaaggcctgc gctttgcgat tcgcgaaggc ggccgtaccg tgggtgccgg
     1141 cgtg
//
LOCUS       DAWEWP010000248         1096 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWEWP010000248 DAWEWP010000000
VERSION     DAWEWP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.248"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            5..946
                     /gene="mafA"
                     /locus_tag="WGN53_002014"
     CDS             5..946
                     /gene="mafA"
                     /locus_tag="WGN53_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0140944.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPETAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            950..>1096
                     /locus_tag="WGN53_002015"
     CDS             950..>1096
                     /locus_tag="WGN53_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140945.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGG"
BASE COUNT          268 a          421 c          242 g          165 t
ORIGIN      
        1 tgccatgaaa accctgcttc tcctcatccc cctcgtccta accgcctgcg gcacactgac
       61 cggcataccc gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac tcgtcgccgc
      121 atcgtcccgc gccgccgtca aagaaatgga tttgtccgcc ctgaaaggac gcaaagccgc
      181 cctttacgtc tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg gacgctactc
      241 tatcgacgca ctgatacgcg gcggctacca caacaacccc gaaagtgcca cccaatacag
      301 ctaccccgcc tacgacacta ccgccaccac caaatccgac gcgctctcca gcgtaaccac
      361 ttccacatcg cttttgaacg cccccgccgc cgccctgacg aaaaacagcg gacgcaaagg
      421 cgaacgctcc gccggactgt ccgtcaacgg cacgggcgac taccgcaacg aaaccctgct
      481 cgccaatccc cgcgacgttt ccttcctgac caacctcatc caaaccgtct tctacctgcg
      541 cggcatcgaa gtcgtaccgc ccgaatacgc cgacaccgac gtattcgtaa ccgtcgacgt
      601 attcggcacc gtccgcagcc gtaccgaact gcacctctac aacgccgaaa cccttaaagc
      661 ccaaaccaag ctcgaatatt tcgccgtcga ccgcgacagc cggaaactgc tgattgcccc
      721 tgaaaccgcc gcctacgaat cccaatacca agaacaatac gccctctgga tgggacctta
      781 cagcgtcggc aaaaccgtca aagcctcaga ccgcctgatg gtcgatttct ccgacatcac
      841 cccctacggc gacacaaccg cccaaaaccg tcccgacttc aaacaaaaca acggtaaaaa
      901 ccccgatgtc ggcaacgaag tcatccgccg ccgcaaagga ggataaaccg tgaaaccgct
      961 gcgcagactg acaaacctcc ttgccgcctg cgccgtagcg gccgccgcct tcggacagcc
     1021 cgccctcgcg gcggacttgg cgcaagatcc gttcattacc gataacgccc aacggcagca
     1081 ctacgaaccc ggcggc
//
LOCUS       DAWEWP010000249         1084 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWEWP010000249 DAWEWP010000000
VERSION     DAWEWP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.249"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(90..641)
                     /locus_tag="WGN53_002016"
     CDS             complement(90..641)
                     /locus_tag="WGN53_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140946.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDLEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFQ
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(695..1084)
                     /locus_tag="WGN53_002017"
                     /pseudo
     CDS             complement(695..1084)
                     /locus_tag="WGN53_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          318 a          343 c          201 g          222 t
ORIGIN      
        1 aaagaaaccg ccattagcac tacacccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgccg gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggt agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatctgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccaaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaactttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaataaggg gctgtcctag ataactagga caaacttgat tttactaatt
      721 gttttaaaat ggaacaagaa cttttatctc actgttgtta aaacgtcgtt cgcactcctt
      781 taaatacagc tcaaaatgcg ctttgggaat gccgttaaac ttgcgtaaat gacgttttgc
      841 ctggttccaa aagttctcaa ttccattaat atggttttgt cgttcagcaa aatgtgtgct
      901 gtgattgata cgaaaacgaa gtttcagcga agctaaaatg gctaaattcg cgcacatcta
      961 atacatcata gctacgataa caatccgtat aaacaatact gtcaggtttc acttgttcac
     1021 ggataatagg aaataaagta gcggtttgag tattcggtac tgtaaccgta taaaccttac
     1081 catt
//
LOCUS       DAWEWP010000250         1070 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWEWP010000250 DAWEWP010000000
VERSION     DAWEWP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.250"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(122..550)
                     /locus_tag="WGN53_002018"
     CDS             complement(122..550)
                     /locus_tag="WGN53_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ0140947.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(531..965)
                     /locus_tag="WGN53_002019"
     CDS             complement(531..965)
                     /locus_tag="WGN53_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ0140948.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          278 a          318 c          191 g          283 t
ORIGIN      
        1 tttgaaaacc ccattaaacc gtctttaacc catcgtcccg aacggtagat gtttcacccc
       61 tgcaaaccaa aaaaaagacc gtctgaatac agacggccaa agcctggtca cacatcactg
      121 cctaaaataa agctgcctgc tgtgccgcag gtcggctcat ttcattgata atcgtatatc
      181 ccgttcgtga agagatgccg tatttagggc atagcttcgt catcgccata agcccgctct
      241 ccttatcaat atcgcgcaat ttgacaaact cctgataaaa cctatggttt ctcaactgta
      301 ttaacgcctt gccgcaccgt gggacataca attcctcgcc accatatacc tgcaacagct
      361 catgtgtttt cacttcgccg atggcttcga ccaaaattgc caaacgctcg gtgtccacct
      421 tgcccttacc aaatttaaac cgcgccccgc caatcgcctt gaccagctgt tccgtcgctg
      481 ccagtccgat gacatccaca atgtccaaca cggtatccgg caataaatgt tcaacttttt
      541 cgaaccccat catccccacc cgctttttcc gttttcctgt tttccgcaat ctgcaacgca
      601 gcaaccagtt tatgtagctg cgtatcgtct aaatattcga ccttatcctt accaaacatc
      661 cgccgcgcca ttgcgtgtgc atagttccaa tgtttgccgc cgacggtcag cagggcttcg
      721 actttgtcca gcattgccgc tgatgatgtc cgacgcagat gcggtttgcc gtgtgggtta
      781 ccttttgctt taggcttaaa tccgtgcgac cgcatatcag cgacaacaga ctcaagttca
      841 gaaacatcca tatccgcaca cgaccgctta tccgtcacac gctccaacac cgcgcgatag
      901 gtaccgtcat ccaagcccag ctccttttga gcaatcttaa ttttcgcaat caacgcccgg
      961 cgcattatct ctctaataca acatatagta taaattagcg cattttatac aataaaatac
     1021 aatatatatt attaagccga tgtttttttg cgaaacggac agacataaaa
//
LOCUS       DAWEWP010000251         1032 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWEWP010000251 DAWEWP010000000
VERSION     DAWEWP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.251"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(299..685)
                     /locus_tag="WGN53_002020"
     CDS             complement(299..685)
                     /locus_tag="WGN53_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ0140949.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGTSARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(682..927)
                     /locus_tag="WGN53_002021"
     CDS             complement(682..927)
                     /locus_tag="WGN53_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ0140950.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMGNTDISRILEDDNETAALFEESAL"
BASE COUNT          278 a          243 c          185 g          326 t
ORIGIN      
        1 cagccatata tattaagtac ctgctcttta tgcgcttttt taggaaagga agatcaaacg
       61 gttcaatagc gaagtcttgc ccaaatttga tggacaggat atagaataca tgcgtgcata
      121 ccacaccctg ccaaagttgg gagatgcact taaggttgca cttgtggtcg ccaactatgc
      181 ctatacacgc ggatttcccg cgtttatagg ccgcgacgga agtctggctt ttaagcgaag
      241 acaataaccg aaaagaccgc ctcggcggtc tttcttgtgt acacagtttt tgtatttgtc
      301 agcttgatgc attgacaact ctaattccat attgcggaat atattcatcg acagtcatca
      361 gttcaaagcc ttccgcttgg gtttgtgcaa tcaacatcct atcgaaaggg tctttgtgta
      421 tttccggaag gcttccagcc tgttttgcat gaaacagacc tataggcagc atttcaaaat
      481 cctcttcttg aagcacatca aaaaactctt ccggtaattt caacaacccc ttgttctgct
      541 tgatggaaat ttcccaaata cttgctgcac tgacaaagat cgcatttctc ggattttcta
      601 tcagtttgcg tgcagatgtc cccagtttct tgtcatccaa caaccaccac agcaaagcat
      661 gggtatcaag cagaatcttt ctcacagagc cgactcctca aataaagctg ccgtttcatt
      721 gtcatcctca agaatacgtg aaatatccgt atttcccata tgactgaatt ttttcaacct
      781 tcctgcattt cgtgccggtt tttcaatacc ggttagttgg acgcaaggct tacctgcttt
      841 cgcaataata acgatttccc ctgcttctgc tctttgaatc aattgactca aattggtttt
      901 tgcctgatga atatttgctt gaaacataac acttctcatg attagctaac ttaactaata
      961 tacatcatta ccaagatttt gggaatctca ttacatatat ttgattatat ccgccgtttt
     1021 attcacacct tg
//
LOCUS       DAWEWP010000252          987 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWEWP010000252 DAWEWP010000000
VERSION     DAWEWP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.252"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(112..744)
                     /gene="mtrR"
                     /locus_tag="WGN53_002022"
     CDS             complement(112..744)
                     /gene="mtrR"
                     /locus_tag="WGN53_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ0140951.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          233 a          215 c          256 g          283 t
ORIGIN      
        1 ctatgttatt gcaaaggtct caatatgtat ttttagaaga aggctttgtg tcattttgat
       61 gccgtctgaa agggtttgtt cgtttcagac ggcattgtta ccaaggcttg attatttccg
      121 gcgcaggtcg ggatggtttt ccaagttgtc catcattatc ccgatgatgc gcggggcggt
      181 tttgcccaaa tcgaaacgtt cgcaggaaga gaaccaccgc caaatcagcc cgtccaaggt
      241 tgatttgatg aagatgaccg ctgtttcctt gtccaaatcg tcagccaaat cctgattttc
      301 caccgcttcg gtcaaaacgg cggtaatttt ctcgcgccag attgcctgat gcttgcgggc
      361 aatggcgata acggcggcgt tttgttccgt gtgttcgcat tttaaaaaca ggatgttgtg
      421 gaatttgtag tagatgtcgt tgctttgcag ccgctcgaaa aagtgcagca gcgtgtggcg
      481 gaataccgcc caagaccctc cttcggcatc ttcggcatct tgcgcgatgc agttttcgat
      541 gtcgtcgcag atacgttgga acagcgcgtc gaacaagtct tccttatttt tgaaatgcca
      601 atagagcgcg ccgcgcgtta cgccggcggc ttgggcgatt tcgttgagcg aggtgcgcgc
      661 aatccctttg cggtaaaagg tttccaaggc ggcaagcatc aggtgttctt tggttttcaa
      721 ggcttcggtt ttggtttttc tcataatggc gttttcgttt cgggtcggtt tgatgagggc
      781 ggattataaa aaagactttg taaccatgca atcgtgtatg tataatgaaa cccatgaaat
      841 tgagactaca tctcaacttt gaaaacaggc ataacctgct ttgcaacccg ttcgaacatt
      901 cgggtatcaa agcaggcggt ttttatatcc tgaaatatag tggattaaca aaaatcagga
      961 caaggcgacg aagccgcaga cagtaca
//
LOCUS       DAWEWP010000253          981 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWEWP010000253 DAWEWP010000000
VERSION     DAWEWP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.253"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..937
                     /gene="odhB"
                     /locus_tag="WGN53_002023"
     CDS             <1..937
                     /gene="odhB"
                     /locus_tag="WGN53_002023"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ0140952.1"
                     /translation="AAEAPAAAPAEAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNAL
                     QGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAGARPEERVPMSRLRARVAERLLASQ
                     QENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGVKLGFMSFFVKAAVAALKKYPVVNA
                     SVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDADQMSIADIEQAIVDYAKKAKDGKIA
                     IEDLTGGTFSITNGGTFGSMMSTPIINPPQSAILGMHATKERAVVENGQVVVRPMMYL
                     ALSYDHRIIDGREAVLTLVAIKDALEDPARLLLDL"
BASE COUNT          237 a          276 c          247 g          221 t
ORIGIN      
        1 cgctgctgaa gcacctgccg ccgctcctgc cgaagctgcc ccggccgccg ctcctgctgc
       61 tgcacaaaac aacgccgcta tgcctgccgc cgccaaactg gctgcagaga gcggtgttga
      121 cgtgaacgca ttgcaaggtt ccggccgtga cggtcgcgta ttgaaagaag acgtacaaaa
      181 tgccgcagcc aaacctgccg cagccgctgc tcctgccgtt gcacttcctg ccggcgcacg
      241 tcctgaagaa cgcgtaccga tgagccgcct gcgtgcccgt gttgcagaac gcctcttggc
      301 ttctcaacaa gaaaacgcca ttctgactac attcaacgaa gtcaacatga aaccaatcat
      361 ggacttgcgt gcgaagtaca aagaaaaatt cgagaaagaa cacggcgtga aactgggctt
      421 tatgtccttc ttcgttaaag ccgctgttgc agccctgaaa aaatacccgg ttgtgaatgc
      481 ttctgttgac ggcaaagaca ttgtgtacca cggctacttc gacatcggta tcgcgattgg
      541 cagcccacgc ggtttggttg tgccaatcct gcgcgatgcc gaccaaatga gcattgccga
      601 catcgaacaa gcaattgttg attacgcgaa aaaagccaaa gacggcaaaa tcgctatcga
      661 agatctgacc ggcggtacat tcagtattac caacggcggt actttcggtt ctatgatgtc
      721 caccccgatc atcaatccgc ctcaatctgc gattttgggt atgcacgcca ctaaagagcg
      781 cgctgtggtt gaaaacggcc aagttgttgt ccgtccaatg atgtatctgg ctctgtctta
      841 cgaccaccgt atcattgacg gccgcgaagc tgtattgacc ttggtagcca ttaaagacgc
      901 gttggaagac ccagcccgcc tgttgttgga tctgtaatcg tttcagacgg ccttttattt
      961 gttaatgaaa aggccgtctg a
//
LOCUS       DAWEWP010000254          958 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWEWP010000254 DAWEWP010000000
VERSION     DAWEWP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.254"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(431..506)
                     /locus_tag="WGN53_002024"
     tRNA            complement(431..506)
                     /locus_tag="WGN53_002024"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(471..473),aa:Ala,seq:tgc)
     gene            complement(512..588)
                     /locus_tag="WGN53_002025"
     tRNA            complement(512..588)
                     /locus_tag="WGN53_002025"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(552..554),aa:Ile,seq:gat)
     gene            complement(689..>958)
                     /locus_tag="WGN53_002026"
     rRNA            complement(689..>958)
                     /locus_tag="WGN53_002026"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          215 a          238 c          195 g          310 t
ORIGIN      
        1 cctgatgcac ttattcactt gactctatca tttcaagaac ttctttgact ttgcctaaca
       61 ttccgttgac tagaacatca gacttgaatt tcctactttg ataaagctta ctgctttgtt
      121 gtgtcttaat cctgcctttt gtgtttcagg attaagtcga tacaatcatc acccaaatac
      181 tgtgtttgtt ttcttttctc ttgtgagcga ttttttatcc tttgcaaaga ataaaaaatc
      241 aaaacaaacg ctttgtcttt gtttgttgat ttcggctttc caatttgtta aagatcgatg
      301 cgttcgatat tgctatctac tgtgcaaatc aaaacgagct gattattata tcagcatttt
      361 gttcttggtc aagtgtgatg tcgccctgaa tggattctgt tccattcttc cgttttgatt
      421 tgtacagtat tggtggaggc aaacgggatc gaaccgatga ccccctgctt gcaaagcagg
      481 tgctctacca actgagctat gcccccgttc ttggtgggtc tgggaggact tgaacctccg
      541 accccacgct tatcaagcgt gtgctctaac cagctgagct acaaacccgg attctcttct
      601 taagcgaatc ttgccttcac tcaagcttct tccgcatctt ttcagtttac cgataagtgt
      661 gaatgcctaa agcctcttct ttctctagaa aggaggtgat ccagccgcag gttcccctac
      721 ggctaccttg ttacgacttc accccagtca tgaagcatac cgtggtaagc ggactccttg
      781 cggttaccct acctacttct ggtatccccc actcccatgg tgtgacgggc ggtgtgtaca
      841 agacccggga acgtattcac cgcagtatgc tgacctgcga ttactagcga ttccgacttc
      901 atgcactcga gttgcagagt gcaatccgga ctacgatcgg ttttgtgaga ttggctcc
//
LOCUS       DAWEWP010000255          943 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWEWP010000255 DAWEWP010000000
VERSION     DAWEWP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.255"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..938
                     /locus_tag="WGN53_002027"
     CDS             <1..938
                     /locus_tag="WGN53_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
                     /protein_id="HEZ0140953.1"
                     /translation="RYHIQYLSRHCTVTEIAKQLNRHKSTISREIRRHRTQGQQYSAE
                     KAQRQSRTIKQRKRQPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHHQITLHHSTIY
                     RYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVGIENRPAIVDQKSRIGD
                     WEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAEDTARAAVRALKAHKDRVHTIT
                     MDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQTDFRNISDR
                     EIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH"
BASE COUNT          303 a          288 c          194 g          158 t
ORIGIN      
        1 aacgatacca catccaatac ctgtcccgcc actgcaccgt caccgaaatc gccaaacagc
       61 tgaaccgcca caaaagcacc atcagccgcg aaatcagacg gcaccgcacc caagggcagc
      121 aatacagcgc cgaaaaagcc cagcggcaaa gccggactat caaacagcgt aagcgacaac
      181 cctataagct cgattcgcag ctgattcagc acatcgacac ccttatccgc cgcaaactca
      241 gtcccgaaca agtatgcgcc tacctgtgca aacaccacca gatcacgctc caccacagca
      301 ccatttaccg ctaccttcgc caagacaaaa gcaacggcag cacgttgtgg caacatctca
      361 gaatatgcag caaaccctac cgcaaacgct acggcagcac atggaccaga ggcaaagtcc
      421 ccaaccgtgt cggcatagaa aaccgacccg ctatcgtcga ccagaaatcc cgtatcggcg
      481 attgggaagc cgacaccatt gtcggcaaag gacagaaaag cgcattattg accttggtcg
      541 aacgcgttac ccgctacacc atcatctgca aattggatag cctcaaagcc gaagacactg
      601 cccgggcagc tgttagggca ttaaaggcac ataaagacag ggtgcacacc attaccatgg
      661 ataacggcaa agagttctac caacacacca aaataaccaa agcattgaaa gcggagactt
      721 atttttgtcg cccttaccat tcttgggaga aagggctgaa tgagaacacc aacggactca
      781 tccggcaata cttccccaaa caaaccgatt tccgtaacat cagtgatcgg gagatacgca
      841 gggttcaaga tgagttgaac caccgaccaa gaaaaacact tggctacgaa acgccaagtg
      901 ttttattctt gaatctgttc caaccactaa tacactagtg ttg
//
LOCUS       DAWEWP010000256          925 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWEWP010000256 DAWEWP010000000
VERSION     DAWEWP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.256"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(398..473)
                     /locus_tag="WGN53_002028"
     tRNA            complement(398..473)
                     /locus_tag="WGN53_002028"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(438..440),aa:Ala,seq:tgc)
     gene            complement(479..555)
                     /locus_tag="WGN53_002029"
     tRNA            complement(479..555)
                     /locus_tag="WGN53_002029"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(519..521),aa:Ile,seq:gat)
     gene            complement(656..>925)
                     /locus_tag="WGN53_002030"
     rRNA            complement(656..>925)
                     /locus_tag="WGN53_002030"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          232 a          228 c          182 g          283 t
ORIGIN      
        1 cctgatgcac ttattcactt gactctatca tttcaagaac ttctttgact tcgtttacct
       61 acctgttgac taagtaaaag caaacttgaa attcctactt tgataaagct tactgctttg
      121 ttgtgtctta atcctgcctt ttgtgtttca ggattaagtc gatacaatca tcacccaaat
      181 actgtgtttg ttttcttttc tctcgcgaga gatttttacc ctttgcaaaa ggtaaaaatc
      241 aaaacaaact cattgtcttt gtttgttgat ttcggctttc caatttgtta aagatcgatg
      301 cgtcgttatt ttactccgca aatcaaaata agttgctata aaatataaaa gtacaaataa
      361 aataaaagca acaggattac tttgatttgc aaagttttgg tggaggcaaa cgggatcgaa
      421 ccgatgaccc cctgcttgca aagcaggtgc tctaccaact gagctatgcc cccgttattg
      481 gtgggtctgg gaggacttga acctccgacc ccacgcttat caagcgtgtg ctctaaccag
      541 ctgagctaca aacccggatt ctcttcttaa gcgaaccttg ccttcactca agcttcttcc
      601 gcatcttttc agtttaccga taagtgtgaa tgcctaaagc ctcttctttc tctagaaagg
      661 aggtgatcca gccgcaggtt cccctacggc taccttgtta cgacttcacc ccagtcatga
      721 agcataccgt ggtaagcgga ccccttgcgg ttatcctacc tacttctggt atcccccact
      781 cccatggtgt gacgggcggt gtgtacaaga cccgggaacg tattcaccgc agtatgctga
      841 cctgcgatta ctagcgattc cgacttcatg cactcgagtt gcagagtgca atccggacta
      901 cgatcggttt tgtgagattg gctcc
//
LOCUS       DAWEWP010000257          905 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWEWP010000257 DAWEWP010000000
VERSION     DAWEWP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.257"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..215
                     /locus_tag="WGN53_002031"
     CDS             <1..215
                     /locus_tag="WGN53_002031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140954.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQGN
                     KPLGAEELADLKPLYKDLKNNIGNIV"
     gene            546..>905
                     /locus_tag="WGN53_002032"
     CDS             546..>905
                     /locus_tag="WGN53_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140955.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT          282 a          147 c          211 g          265 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacaca tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg aggagctggc ggatttgaaa ccactttaca
      181 aagacttaaa gaataatatt ggaaatattg tatgaacaaa aaattaaact atatttttat
      241 gttggactgt ttagggttgg tgatattgtt tacttgtata atagctactt ttgaaagaga
      301 ttatggattt aaaattttta ctaattctaa gaggcctgaa ttttattatt ggattggaat
      361 gttttattat ggaattattt cttgctggtt tgattatcaa ttaatttcaa caaaggcgaa
      421 ttcgtataaa agaaaagtta aacaatataa aattttttca gtaatatttt cagttttgat
      481 atttatttct actatagtaa aactttaaat tttggagcaa aaatttatga gcgattcaat
      541 tgaatatgta ttgggaacgc ggtctgcaca tgtataaggc aagtgctgtc gtgccaacgg
      601 gatatgtacg ggttgggaat accgcgccgc tggtcggcga agacacgcaa cggtatgcct
      661 ctttttgggg cgacggctac gacgtgtacc gtcagttgag atggcagcag atacccgaaa
      721 aacagagaaa ggcattcaaa aaagccgcca aaagcaaaaa gaccgtgatg tttgccggac
      781 gggaatacgg catatccaaa cagaatttga gcgatgtttg ggatgatttt gaagacgcga
      841 tggaactgaa ggcgtttccc tgcctgtctt cgctgtttct gaccaagtgg cataaaaatc
      901 tatat
//
LOCUS       DAWEWP010000258          895 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWEWP010000258 DAWEWP010000000
VERSION     DAWEWP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.258"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(152..850)
                     /locus_tag="WGN53_002033"
     CDS             complement(152..850)
                     /locus_tag="WGN53_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0140956.1"
                     /translation="MQADLAYAAERITHNYPEPTGADKDKISTVSDYFRNIRTHSIHP
                     RVSVGYDFGGWRIAADYASYRKWNNNKYSANTKELQRNNNSGNWQELKTENQENGSFH
                     AASSLGLSAIYDFKLNDKFDKFKPYIGARVAYGHVKHQVHSVRKETTTTFIKPGAGAT
                     KPGKIILDSPPKPAYHESHSISSVGLGVIAGVGFDITSNLTLDAGYRYHNWGRLENTR
                     FKTHEVSLGVRYRF"
BASE COUNT          183 a          200 c          251 g          261 t
ORIGIN      
        1 agattgcggt gttgtcggga atgacgggtt tcaagattac ggtgttgtcg ggaatgacgg
       61 tttgggtatt tccacgcccg ccccgtgtct gtaaacggca agtgcatcaa aaatgccgtc
      121 tgaaggttca gacggcatcg gtatcgggga atcagaagcg gtagcgcacg cccaatgaga
      181 cttcgtgggt tttgaagcgg gtgttttcca agcgtcccca gttgtggtaa cggtagccag
      241 catctaaagt cagattggac gtgatgtcga aaccgacacc ggcgatgaca ccaagaccca
      301 cgctgctgat gctgtggctt tcgtgataag caggtttggg gggcgaatct aatataatct
      361 tgcctggctt tgtggcgcct gcccctggtt taatgaaagt agtcgtggtt tcttttctca
      421 ccgaatgaac ctgatgttta acgtgtccgt aggcgacgcg cgcaccgata tatggtttga
      481 atttatcgaa tttatcgttg agtttgaaat cgtaaatggc ggataagccg agagaagaag
      541 cggcgtggaa gctgccgttt tcctgatttt ccgtcttcag ttcttgccag ttgccactgt
      601 tattgtttct ttgcaactct tttgtgttgg cggaatattt attgttgttc cactttctgt
      661 aactggcata atctgccgct attctccagc cgccgaaatc gtagccgacc gacacccggg
      721 ggtggatgga atgcgtacga atgtttctga aataatcgct tactgtgctt attttgtctt
      781 tgtctgcacc ggttggttcc ggataattgt gggtaatgcg ttcggcggca taggctaaat
      841 ccgcctgcac ataatacggg ctgcggctgc tgtcttcact tgccgcctgc gctgc
//
LOCUS       DAWEWP010000259          870 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWEWP010000259 DAWEWP010000000
VERSION     DAWEWP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.259"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..346)
                     /locus_tag="WGN53_002034"
     CDS             complement(<1..346)
                     /locus_tag="WGN53_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0140957.1"
                     /translation="MFLILGRNFLKIILCFSFFVPKFALASVNVPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLVR"
BASE COUNT          332 a          189 c          111 g          238 t
ORIGIN      
        1 agcggacgag tttgccgacc cccgccaata cgccggcgcg ggaaactgat gaagatacaa
       61 ccgaactaac tttacccgta cttgatgctc cagtagatac atgcctaact tttaaatctt
      121 gagcttctga agttaaatat cttcccgatt ctctatcaaa tacacctttc caaattcttc
      181 ttcttttgtc atctgaacct cgaataccta aatatctgcc atcatcataa acttcaaccc
      241 tatcaaattt acccggaaca tttactgatg ccaatgcaaa tttaggtaca aaaaaactaa
      301 agcataggat aatctttaaa aaatttcgcc ctaggataag aaacataaca ttttgcttca
      361 tctaatttaa ttaataatgt agaagaatca gggaattgaa tttctataaa atctctatca
      421 taacgatgaa agaaatttct aaaaatatca aatgaaaacg aagatttaaa aacccctttt
      481 ctaatcaatt ccctataata aattaacgct tgtggtaaat ctctataaat atgtgaatca
      541 gtttgaaatt taactaaatg atattcagaa ataaacataa ttcggacctc ttaaaatcac
      601 tctaaaaatc cgattctacc aaccgttacc aaaacagcca agcaacagac aaagccaaga
      661 agaaacctag aagaaaccaa aaatcaataa ccatcaatca gtaccaacct tgcctatgtc
      721 ttttaaaaaa ttaatcaaaa gcctgaagcc gtaaatgact acgaacagaa ttaaaaccgt
      781 cgaaccgaca taagaaccct gtttgatctg ctcaaaattg gaacatttcg gataggacaa
      841 cattaccggc tttccgttca agacccattt
//
LOCUS       DAWEWP010000260          867 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWEWP010000260 DAWEWP010000000
VERSION     DAWEWP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.260"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(48..827)
                     /locus_tag="WGN53_002035"
     CDS             complement(48..827)
                     /locus_tag="WGN53_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ0140958.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          200 a          288 c          173 g          206 t
ORIGIN      
        1 ttcactatac cgatacatcc gaatatgcca gaccgtctaa acggtattca tacctcccaa
       61 tcctgcacgc gcttcaaatc gttttgcgcc aaagtatccg cgtggcggtt acggctctga
      121 tattccctgt ctttcatgat gctgcttgcc acataattca aatgtgcctt tgccgcctcc
      181 gaagcctcgc ccggccgacg gttcgatatc gcctcataca gcacgcggtg ctgcgccatc
      241 agtttcggac gcggatcttc ttcctgattc agataaataa ggctgctgcg cgtctgccgg
      301 tacagcattt tcaacaaacc gcccgacaaa tggctgaaca acaaattgtg cgccgcatcc
      361 gcaatcgtct gatggaagct gacatcggct tcgctctgat gttccagatt gccgctttcg
      421 cacgcctcct caaacttttc aagccaaaac ccaatccgct tcaaatcggc atccgtccgg
      481 cgttctgccg ccaatgccgc catacagccc tcgatgtggc agctgaaatc aaaaacatcc
      541 tgttcccaat tggaatgctt gcccaaaagc tcctgccaac tttgcaaaaa atcctgctgc
      601 ggcttgaccg aaacataata accgtctccc tgcctcgctt ccaaaacctg acgggcgacc
      661 aaaacattca atgccgacct gaccgacggg cgggacacgc cgaactcttc cgccaaaacg
      721 cgttcgggcg gaatcttgcc cccttccgcg taaacccctt ccacgatgcg gccttccaat
      781 accgacaata cctgatcgct gattttctga ggccttacca gtttcatcac tcctccttta
      841 taaagattcc ctgccgaacc cttccga
//
LOCUS       DAWEWP010000261          848 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWEWP010000261 DAWEWP010000000
VERSION     DAWEWP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.261"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1..400)
                     /locus_tag="WGN53_002036"
                     /pseudo
     CDS             complement(1..400)
                     /locus_tag="WGN53_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573927.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(521..832)
                     /gene="rpsJ"
                     /locus_tag="WGN53_002037"
     CDS             complement(521..832)
                     /gene="rpsJ"
                     /locus_tag="WGN53_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ0140959.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLSAGVDVEIKV
                     Q"
BASE COUNT          198 a          192 c          214 g          244 t
ORIGIN      
        1 ggccgctttc aacaggttca aacacatcgc tttcaggtgg ctttgcgcac tcactttgag
       61 cagcccgaaa taggctgccc gcgcatagcg gaatttacgg tgcagcgtac cgaagctttg
      121 ttcgaccaca taacggggct tcgacaaata tcggttgcgt ttggtttgcg cttccgacag
      181 cggacggttg cggcaggctt tgcgcataat gccgtccagc aactgatgtt ctttcagatg
      241 ttgccggttt tccgcactgt catagccttt atcggcatag atggtcgtac ctttgctcga
      301 tcggctgcca atcaatcact tgatccaact tcaatagcgg gaaacggtcg atgtgtttgg
      361 caatcatggc ttgtgcggtt tgttgaaaga aggtgctcat gagaaatccc ctaaatgtct
      421 tggtgggaat ttaggagatt ttggggaatt ttgcaaaggt ctcggcataa cataaaaaat
      481 ccccaagcat tgattgcttg gggattttca atagtccgaa ttattggact ttgatttcta
      541 catcaacacc ggccgacaaa tccagcttca tcagcgcatc ggtagtttta tcggtccaat
      601 ccacgatgtc catcaggcgc aagtgggtgc ggatttccaa ttgttcacgg gaagttttgt
      661 tcacgtgcgg agaacgcaaa atgttgaaac gctcgatttt ggtcggcaaa ggaatcgggc
      721 cttttacaac ggcaccggta cgttttgcag tttcaacgat ttcttgtgca gaacggtcaa
      781 tcagggcgta atcataagct ttcaggcgga tacggatttt ttggtttgcc atttatcaat
      841 atccttca
//
LOCUS       DAWEWP010000262          843 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWEWP010000262 DAWEWP010000000
VERSION     DAWEWP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.262"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..247)
                     /locus_tag="WGN53_002038"
     CDS             complement(<1..247)
                     /locus_tag="WGN53_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0140960.1"
                     /translation="MCCSLSFAEPARIDDRIIKFRPSKSKFFESTGYRKINNEFSKFT
                     EAANVEHIPTGAKARINAKITASVSRAAVLSGVGKLVR"
     gene            complement(231..551)
                     /locus_tag="WGN53_002039"
     CDS             complement(231..551)
                     /locus_tag="WGN53_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0140961.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
BASE COUNT          268 a          173 c          142 g          260 t
ORIGIN      
        1 ggcggacaag tttgccgact cctgacaaga cggcggcgcg ggatacgctg gcggttatct
       61 ttgcgttgat tcgggctttt gcgcccgtgg ggatatgttc gacatttgcc gcttctgtga
      121 atttagaaaa ttcattattg atttttctat atcctgtaga ttcaaaaaac tttgatttag
      181 atggcctaaa ttttattatt cgatcatcta ttcttgctgg ttctgcaaaa ctaagagaac
      241 aacacattaa aatcggtgtt gctatcagaa aattccgagt aaataaattc atgcgaaatt
      301 tttccatttt cttctgactt cctaataaaa atattagact catcagaaaa ataaattttc
      361 caaatattat gcgttaccct cttatttaaa aaataagaga aacattctat tacgtcgtat
      421 tcttttacat ttcttacaaa ctcttcaaat tccttagacg caattaatgc catcgactgc
      481 ccaaaatact tgctggacgg ctgatattta taaagtgcca actgcgcctg cgtgataaac
      541 ggcttgttca tgtttctgtc tttcaaaggt tgttttgaaa gcctgatttt aaaacacgtc
      601 atataaatat caaagcgaca gacaaagcca ggaaaaatcc gagcaaaaac caaaaatcga
      661 caaacatcat cacgccccta ctttgcctat gtcttttaag aaattaatca gcagcctgaa
      721 gccgtaaata acgacaaaca gaattaaaac catagacccg agataagctc cggatttaac
      781 ttgttcgtaa ttcgaacatt tcggataaga cagcgtgacc ggctttccgt tcaaaatcca
      841 ttt
//
LOCUS       DAWEWP010000263          804 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWEWP010000263 DAWEWP010000000
VERSION     DAWEWP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.263"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            371..655
                     /locus_tag="WGN53_002040"
     CDS             371..655
                     /locus_tag="WGN53_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0140962.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          239 a          150 c          206 g          209 t
ORIGIN      
        1 aaaacagcaa ccgctccgcc gtcattcccg cgcaggcggg aatccagaac gtaaaatcta
       61 aagaaaccgt gttgtaacgg cagaccgatg ccgtcattcc cgcgtaggcg ggaatctaga
      121 ccttgggata acggcaatat tcaaagttta taaaagaccc gttattcccg cgcaggcggg
      181 aatctagacc ttagaacaac agtaatattc aaaggttagc tgaagcttta gagattctag
      241 attcccactt tcgtgggaat gacgaagagt tgcgggaatg atggaaagct atgggaataa
      301 cgaaaggtta aagtaatcac gggatggtgt tcgcgggaat ataaatttaa ataattcaaa
      361 agggtattat atgcagcctg cggtttatat tttagcaagc caacgtaatg gcacgttata
      421 cattggcgtt acatctgatt tggtgcaacg tatttaccaa catagggaac atttgattga
      481 gggatttaca tcacggtaca acgttactat gctggtttgg tatgaactgc atcctacgat
      541 ggagagcgca attactcggg aaaaacagtt gaagaaatgg aacagggctt ggaaattgca
      601 actgattgaa gaaaataatg tttcttggca ggatttatgg tttgatatta tttagcccgt
      661 cattcccgcg caggcgggaa tctagaacca tcggcacgga aacttatcgg gtaaacggtt
      721 tcttgagatt ccgcgtccta gattcccact ttcgtgggaa tgacgggatt ttaggttcgc
      781 gggaatgacg ggatttgaga ttgc
//
LOCUS       DAWEWP010000264          789 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWEWP010000264 DAWEWP010000000
VERSION     DAWEWP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.264"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..234
                     /locus_tag="WGN53_002041"
     CDS             <1..234
                     /locus_tag="WGN53_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0140963.1"
                     /translation="LIAQKEFEEFVNNVKEYDILESFSYFLNKRVAHNIWKIYFSDES
                     VIFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
     gene            176..616
                     /locus_tag="WGN53_002042"
     CDS             176..616
                     /locus_tag="WGN53_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140964.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLERSQIKFQSSNNLKSS
                     GFKLDSSSKSFAKFIEAANFEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKF
                     STRAVPYVGTALLAHDVYETFKEDIQARGYQYDPETDKFAKVSG"
BASE COUNT          248 a          141 c          180 g          220 t
ORIGIN      
        1 ttaattgctc agaaagaatt tgaagaattt gttaataacg taaaagagta cgatatttta
       61 gagagtttct cttatttctt aaataagaga gtcgcacata atatttggaa gatttatttt
      121 tctgatgaat ctgttatttt tataagaaaa tcagaagaga acggaaaaac tgttcatgaa
      181 ttcgtttatc aagagtacac cgatagttct gattttaatt ctatgttcga gtaattctta
      241 ttctgaacct gtaagattag aaagatcaca aattaaattt caatcaagta ataatttaaa
      301 atcaagtggt tttaaattag attcaagttc taagagtttt gccaaattca tagaagcggc
      361 aaattttgag cacatcccta cgggcgcaaa agcccgaatc aacgccaaaa taaccgccag
      421 cgtttcccgc gccggcgtat tggcgggggt cggcaaactt gcccgcttag gcgcgaaatt
      481 cagcacaagg gcggttccct atgtcggaac agccctttta gcccacgacg tatacgaaac
      541 tttcaaagaa gacatacagg cacgaggcta ccaatacgac cccgaaaccg acaaatttgc
      601 aaaggtctca ggctaagtgc gcctgttgcc gcctaaaagg taacccggat gcctgattat
      661 cgggtatccg gggaggatta agggggtatt tgggtagaat tagggagtga ttggtagcgg
      721 aaatagacga aaacctgtgt ttgggtttcg gctgtcggga gggaaaggaa ttttgcaaag
      781 gtctctttt
//
LOCUS       DAWEWP010000265          752 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWEWP010000265 DAWEWP010000000
VERSION     DAWEWP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.265"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            82..>752
                     /locus_tag="WGN53_002043"
     CDS             82..>752
                     /locus_tag="WGN53_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0140965.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCITARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLP"
BASE COUNT          230 a          202 c          161 g          159 t
ORIGIN      
        1 aatttttaaa catatatcct tgatatatac acggcgtaaa catatactgg aaacatcttt
       61 aaattttccg aaattttaaa tatgagcaac tggaaacccg atattcccta taacgattta
      121 ccacccctgc cgccaaaaca ggatattgaa agcaaaacca tcctgaaacg ttgtataacc
      181 gcccgtgcat cccttgcccg tttaaagcag gcggcagaac tgataccgaa tcaagccatg
      241 ctgattaaca cccttcctgt tatggaagcc cgtgcaagtt cggaaattga aaacatcgta
      301 accacaacgg ataagctgtt tcaatccctg caaatggata cggaacggca agaccctgcc
      361 acgaaagaag ccctgcaata ccgcaccgcc ctgtttgcag gctatgaatc actgacgagc
      421 cgccctttat gcacacaaac cgccatcatg gtctgcaacg ccatcaagca cccctacgaa
      481 atggccatcc gcaaaacagg cggcacagcc ctaaaaggag gcaacagcgg aaatgttgtc
      541 tataccccgc ccgaaggaga agaaaccata cgcggcaagc tggcaaattg ggagcggttt
      601 attcacgaaa gcggcgattt agacccgctt atcatcatgg cggcggcaca ttaccaattt
      661 gaagccatcc atccgtttac ggacggcaac gggcggacgg ggcgcatatt gaacagcctg
      721 ctattgattg aaaaagggct tttggatttg cc
//
LOCUS       DAWEWP010000266          724 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWEWP010000266 DAWEWP010000000
VERSION     DAWEWP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.266"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(127..>724)
                     /locus_tag="WGN53_002044"
     CDS             complement(127..>724)
                     /locus_tag="WGN53_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0140966.1"
                     /translation="LSIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPV
                     QSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGV
                     DIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQV
                     KRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          161 a          205 c          196 g          162 t
ORIGIN      
        1 aaacacagtc aaacaaattt atatgcccca tcccttccga ataatttgaa aacacagccg
       61 ccaaaaacaa aaatgccgtc tgaaaacctt tcagacggca tttccaattt gatttcaggc
      121 agaaagtcag aacgcgatat agctgttcgg gttaaccggt ttgccgtttt gccgcacctc
      181 gaaatgaagc tgcgttctgg aagcgtcggt attgcccatc aaagcgacct gctgcccgcg
      241 tttgacctgc tggccttcgc cgaccagcaa tttttggttg tgcccgtatg cggtcaggaa
      301 ggaagaatta tgctggatga tgaccagatt gccgtatccc ctcaaaccgg aacctgcata
      361 aaccactttg ccgtcagccg ccgccaaaac gggttgtccc gcatttcctg caatatcgac
      421 acccttgttg ttgccgccga aatcggcaac cactttacct tgcgtcggac gctgccaaac
      481 aatgccgccg accgaacgcg ttccggaagg cgaagcggca ggagattgcg gggcgggcgc
      541 gggaaccgct ttattttccg cagcgggcgc ggcaggttgc ggcgcggact gcacaggcgg
      601 ttgcgcggcg ggtttcacag gggtttgcac ggcagccggt acggcgggcc tgctttctac
      661 ggctgaggtt ttcggtgcgg catatcctgc cggtttgact ttaacaatct gaccgatgct
      721 caac
//
LOCUS       DAWEWP010000267          721 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWEWP010000267 DAWEWP010000000
VERSION     DAWEWP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.267"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(305..>721)
                     /locus_tag="WGN53_002045"
     CDS             complement(305..>721)
                     /locus_tag="WGN53_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0140967.1"
                     /translation="IIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKID
                     AIRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKEL
                     SDIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSF"
BASE COUNT          162 a          164 c          171 g          224 t
ORIGIN      
        1 aatagttacc ttttcttatt taaaaatagc tttctcaaat tccatgaacg cctcaatacg
       61 atatgcagat gctctatcga aattaagttt caacattttg tttattaaac attttatttt
      121 agccattttt caatataccc ccaaatatac ccccaatttg cacaagtcaa aattttaggc
      181 aggggttttg ttgctcccat gatacgcggg cggatgccgg cttttcgcgg gggaaataca
      241 aggggtctcg gttcgggtgt caaaatccct gtttcgtgtt agtcatgtgg gggggaagag
      301 ggggttagaa tgaagtaaag ctgttgccct ctccccgcaa tagttccatt aggcgcggat
      361 gaatgaatag tttgtccctg ccgatgacga tttcttgcag cacacctatg tctgaaagct
      421 ctttcaggta cttagaggcc gtctgccgtt tggctatccc tgccgcttct aggttggcaa
      481 tgcgtgtata tggctgctca aacagaagat ttaccagttc gtgcgtgtag attccttgtg
      541 cgtgtgtccg tatgtgttgc cgtgtctgct cgaacaggcg gcgtatcgca tctattttcg
      601 ataccgtcca atcggcggtg tcggctacgc cgtctaagat gtagattatc cagctttccc
      661 aatcctgccg ttcggttacg cctaaaagca ggcggtaata gtccgccctg ttttcgatga
      721 t
//
LOCUS       DAWEWP010000268          704 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWEWP010000268 DAWEWP010000000
VERSION     DAWEWP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.268"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            27..704
                     /locus_tag="WGN53_002046"
     CDS             27..704
                     /locus_tag="WGN53_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HEZ0140968.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
BASE COUNT          218 a          136 c          156 g          194 t
ORIGIN      
        1 atcaaaaaac ctgataagct cctaatatga aactaaaaaa taagtatcaa aagttcagca
       61 aaatttcaga gcaaaagttt agagaaataa tccgctgttt cgctcttgat ttgaccgctt
      121 ccgatactgc taaaatgacc ggcattagtg tacgcggtat caatcctatt ttcctgaaaa
      181 tcagacacag gattgctgct ctgtgcgaac aaagctcacc actgtccggt gtggtcgaat
      241 tagatgaatc ttactttggt gcgcgacgta tcaggggtaa gcgcggtcgc ggagcgtcag
      301 gtaaaaccat cgtatttggc atactgaaac gcaatggcgt ggtctatacg gaaatcgttc
      361 ccgacgcttc gaaagcctca ctaatcaagg tcatacgtgg tcacatatct gctgacagtg
      421 aaatcaatac tgacggctgg aaagcatatg acggtcttgt cgatatgggc tatgagaagc
      481 attaccgtgt ccatcatggt tccaatgagt ttgctcgagg aaagcaacat atcaatggta
      541 ttgaatcttt ttggagttat actaaggggc gtttggctaa atttcatggt atttccaaag
      601 agatgtttta tctgcatctc aaagaaacgg agtttaggtt taaccaccgg catgaagatt
      661 taggtaaaat attaatgaag atgcttcgaa ataacccaat ttaa
//
LOCUS       DAWEWP010000269          652 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWEWP010000269 DAWEWP010000000
VERSION     DAWEWP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.269"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WGN53_002047"
     CDS             complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WGN53_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ0140969.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          195 a          211 c          116 g          130 t
ORIGIN      
        1 tcaaaccgga aaaatgccgt ctgaatcttc agacggcatt ccattcatat ttattcgtct
       61 tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt aaggctcttc aaccgtatat
      121 tccccctcga taatatcgtc atcgcgggaa aagccctctt ttctgcccga ttggttcatg
      181 ttgaaaaaat tttccgcacc tcctgcctgc aacactgcac ctcccttaaa cggcagcagc
      241 agcaataccg ccaacaccga ggatacgaat cccggactca tcagacacac cgccgccacc
      301 gtataacgga taggccacaa catctgataa acggataccc tcccgccgct tctcattgcc
      361 gcgcccgcca ataaaagacc ggacagcccc gtatgcctga gcatcagcac accggcggca
      421 aaacctgccg ccatcaaaaa caacgtccag ccgccgccca accaatcggc aacccacaca
      481 atcgacataa tctccaaaaa cagcagcacc aaaaaaccga taccgaaaaa tctcattgac
      541 cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac aagactccaa gcgacctgcc
      601 tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa tgtgaaatca cg
//
LOCUS       DAWEWP010000270          607 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWEWP010000270 DAWEWP010000000
VERSION     DAWEWP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.270"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..555
                     /locus_tag="WGN53_002048"
     CDS             <1..555
                     /locus_tag="WGN53_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0140970.1"
                     /translation="VSVGYDFGGWRIAADYARYRKWNNNKYSVNTKNVQKNDNGNRQD
                     LKTENQENGTFHAVSSLGLSAIYDFKLNDKFDKFKPYIGVRVAYGHVRHSIDSTKKTT
                     NVVTVAGAANTAPTIYYAPETQNAYHESHSIRRLGLGVIAGVGFDITPKLTLDTGYRY
                     HNWGRLENTRFKTHEVSLGMRYRF"
BASE COUNT          179 a          170 c          128 g          130 t
ORIGIN      
        1 gtgtcggtcg gctacgactt tggcggctgg agaatagcgg cagattatgc ccgttacaga
       61 aaatggaaca acaataaata ttccgtcaac acaaaaaatg tgcaaaaaaa cgacaatggc
      121 aacaggcaag acctgaagac ggaaaatcag gaaaacggta cattccacgc cgtttcttct
      181 ctcggcttat ccgccattta cgatttcaaa ctcaacgata aattcgataa attcaaaccc
      241 tatatcggtg tgcgcgtcgc ctacggacac gtcagacaca gcatcgattc gaccaaaaaa
      301 acaacaaatg ttgttaccgt cgccggtgct gctaacacag cacctacgat ttattatgca
      361 ccagagacgc aaaacgccta tcacgaaagc cacagcatcc gccgcttggg tcttggtgtc
      421 atcgccggtg tcggtttcga catcacgccc aagctgaccc tggacaccgg ataccgttac
      481 cacaactggg gacgcttgga aaacacccgc ttcaaaaccc acgaagtctc attgggcatg
      541 cgctaccgct tctgattccc cgataccgat gccgtctgaa ccttcagacg gcatttttta
      601 cacaatt
//
LOCUS       DAWEWP010000271          599 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWEWP010000271 DAWEWP010000000
VERSION     DAWEWP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.271"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..150
                     /locus_tag="WGN53_002049"
     CDS             <1..150
                     /locus_tag="WGN53_002049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140971.1"
                     /translation="SIRQGKPEEEFSRKMAGCLTDLENCLEKYLEQFGPVSESIEACT
                     VKLQE"
     gene            172..465
                     /locus_tag="WGN53_002050"
     CDS             172..465
                     /locus_tag="WGN53_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140972.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPDCFDMKAVWQMFNRIPR"
BASE COUNT          158 a          150 c          160 g          131 t
ORIGIN      
        1 tccattcggc agggaaagcc tgaagaagaa ttttcccgta agatggccgg ctgcctgacc
       61 gacttggaaa actgtttgga aaaatacctc gaacagttcg ggccggtatc ggaaagtatc
      121 gaagcctgta ccgtcaaact gcaggagtaa ccgtcgttct tcaacaggct gatgaaaacc
      181 aacgacaagc tcaataggca gattgacgtg ttgcagaaac agtcggcagc catccacaac
      241 gaagcctata tcgaaatgaa cacgctgctt taccgccacc gcgaagtggt ttccgtccac
      301 aaccgcaagg cggattatgc ggaaaagggc aaagagcgga ttgcgctgtt cccgcgcggc
      361 ttgaacggca tcaccaagct gcctgccgcc gtcctgttgc ccgagcgtcc cgattgtttt
      421 gatatgaagg cagtttggca aatgtttaat agaataccga ggtaggcgta tcggctgccg
      481 gataaaaatg ccgtctgaaa cggaagcgtt cagacggcat tgttgcagtg gaacaaatca
      541 aaatgcaccc cgtcattcct gtctgtacgg tgatagcggc agtgttttcc tgcttcgtt
//
LOCUS       DAWEWP010000272          598 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWEWP010000272 DAWEWP010000000
VERSION     DAWEWP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.272"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            308..>598
                     /locus_tag="WGN53_002051"
     CDS             308..>598
                     /locus_tag="WGN53_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0140973.1"
                     /translation="MQVFLTIPGYDVEAEIEKFVWMDAVIWQMPSWWMHEPWTVKKYI
                     DEVLTAGHGKLYQSDGRHSVNPTEGYGTGGLLQGKKHMLSLTWNAPIEAFTRE"
BASE COUNT          174 a          149 c          147 g          128 t
ORIGIN      
        1 actcattagt tacctaaaac ttaaaaagca gaaaccttta cgccgtcatt cccacgaaag
       61 tgggaatccg ggaacttaac gttacagtga tttatcggaa acggctgaaa ccgaatgaat
      121 tggattcccg cctgcgcggg aatgacgaat ccatccgtac ggaaacctgc accacgtcat
      181 tcccactaac ctacattccg tcattcccac gaaagtggga atccaggacg aaaaatctcc
      241 agaaaccgtt ttacccgata agtttccgca ccgacaggtc tggattcccg cctgcgcggg
      301 aatgacgatg caggtatttc tgacgattcc cggctatgat gttgaggcag aaatcgaaaa
      361 attcgtttgg atggatgctg tgatttggca gatgccgagc tggtggatgc acgagccttg
      421 gacagtgaaa aaatacatag acgaagtatt aaccgctgga cacggcaaac tctaccaaag
      481 cgacggcaga cacagcgtca atccgactga gggctacggc acaggcggct tgttgcaagg
      541 caaaaaacat atgctttcac tgacttggaa tgcgccgatt gaggcgttta cccgcgaa
//
LOCUS       DAWEWP010000273          596 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWEWP010000273 DAWEWP010000000
VERSION     DAWEWP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.273"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..379)
                     /locus_tag="WGN53_002052"
     CDS             complement(<1..379)
                     /locus_tag="WGN53_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140974.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKF"
     gene            complement(363..>596)
                     /locus_tag="WGN53_002053"
     CDS             complement(363..>596)
                     /locus_tag="WGN53_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0140975.1"
                     /translation="VIAQSEFVEFAKINKSENVIDCFSFFWNRRIKHDIWLISFSDNS
                     EMVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
BASE COUNT          166 a          118 c          111 g          201 t
ORIGIN      
        1 caaatttgtc ggtttcggtg tcgtattggt agcctcgtgc ctgtatgtct tctttgaaag
       61 tttcgtatac gtcatgggct aaaagggctg ttccgacata gggaaccgcc cttgtgctga
      121 atttcgcgcc taagcgggca agtttgccgc cccccgccaa tacgccggcg cgggaaatgc
      181 tggcggttat tttggcgttg attcgggctt ttgcgcccgt agggatgtgt gttaaatcta
      241 ccgtttttat taaatcagat gaataagttt tactattttt aggtacaaac ttatgaattt
      301 tcgcaccttg tccggtatca actgaaacag tttcagatat ttttactgca tttgcattcg
      361 cttcaaacga atacatcatc aaaattgcaa ttatcgacaa tttcgcaaaa ttcaaatttg
      421 tatattttat gaccatcttt cagggattct ttaattacca tttctgaatt atcagaaaat
      481 gagattagcc aaatatcatg tttaattctt ctattccaga aaaaagagaa acaatcaata
      541 acattttcag acttattaat cttcgcaaat tcaacaaatt cagattgcgc tataac
//
LOCUS       DAWEWP010000274          573 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWEWP010000274 DAWEWP010000000
VERSION     DAWEWP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.274"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1..146)
                     /locus_tag="WGN53_002054"
                     /pseudo
     CDS             complement(1..146)
                     /locus_tag="WGN53_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
     gene            complement(169..354)
                     /locus_tag="WGN53_002055"
     CDS             complement(169..354)
                     /locus_tag="WGN53_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140976.1"
                     /translation="MGVCLDACCSAAGIARQIGKGGEIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPRMLGKS"
BASE COUNT          142 a          155 c          145 g          131 t
ORIGIN      
        1 tccactatat tttgatgcag acgtgtaccg atgtagcccg gctgggtttt caggaaatcg
       61 cgtgatttct gccaagcagc caaagtttcg gcttctttgc cctgcggcac ttcaaaggtg
      121 ttgatgagta cagcactgtt ctgcatgatt taaatttaat ccactatatt aagattttcc
      181 gagcattcga ggcagcacgg attcgccttt gatgtggtgg taggcaacca ttccaatgtg
      241 tcccaacgac aaaaacagca atgaaaatgc cagcgtgccg tgaatttctc cgcccttgcc
      301 gatttgacgg gcaatgcctg cggcggaaca gcaagcatca aggcatacgc ccaatgctca
      361 atttggtgac ggcggtgttg gcggggcggt ttttacgttg gcggattccc cacccttgta
      421 tatcggaact cctgtatcat agcaacaaac cgcccgaccg ccacccgcgc ccaccaaaag
      481 cagacaaccg ttgcgtagct cagggagcgg cagggtaacc catcgacaca accggacagt
      541 tgccggacaa cacaaccgaa tgcaaggcag gtt
//
LOCUS       DAWEWP010000275          530 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWEWP010000275 DAWEWP010000000
VERSION     DAWEWP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.275"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          124 a          151 c          127 g          128 t
ORIGIN      
        1 cgccgttttc accgaatccg ccccttttca caatccagtc ctacgcaact gaaccgtcat
       61 tcccgcgcag gcgggaatcc ggacttgtcg gcacggaaac ttatcgggta caacggtttc
      121 ttgagattcc gcatcctaga ttcccgcctg cgcgggaatg acggcgcata agtttctgtg
      181 ttgatttgag tctatgggaa cggtgtagga ttggtggaat cgttggaatc ggtgggctga
      241 agcccaccct ataactcacc ttttacgaat cctcctacac cctatacaac accttgaaaa
      301 tccaccttgt cgggaatgcc cgaaccgtca gaaatacccg aatcgtcatt cccgcgcagg
      361 cgggaatcca gacctgtcgg cacagaaact tatcgggcaa aacggtttct tgagattccg
      421 catcctagat tcccgcctgc gcgggaatga cggctgcaga tgcccaacgg tctttattcc
      481 tccatctcta agatttacag cgatacacgg gtagtttaag gaatgtccga
//
LOCUS       DAWEWP010000276          520 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWEWP010000276 DAWEWP010000000
VERSION     DAWEWP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.276"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1..520
                     /locus_tag="WGN53_002056"
                     /pseudo
     CDS             1..520
                     /locus_tag="WGN53_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          165 a          137 c          127 g           91 t
ORIGIN      
        1 aactggctgg cgcaagacga caccctgtcc gaactgttgg aactgatcaa ccgccaactg
       61 accgaaaaag gtttaaaaat agagaaagca tccgctgccg ttgttgacgc caccattatt
      121 cagaccgccg gcagcacaca gcgtcaggct atagaagtcg atgaagaagg acaaatcaac
      181 ggccaaacca caccgagtaa ggacaaagat gcccgttgga tcaagaaaaa cggcctctac
      241 aaactcggtt acaaacaaca tacccgtacc gatgcggaag gctatatcga gaaactgcac
      301 atcacccccg ccaatgccca tgagtgcaaa cacctgtcgc cgttgttaga agggatagct
      361 gaaagtacga ccgtctatgc cgacaaaggc tatgacagtg cggaaaaccg gcaacatctg
      421 aaagaacatc agttgttgga cggcattatg cgcgggcagc ctatttcgga ctgctcaaag
      481 tgagtgcgca aagccacctg aaagcgatgt gtttgaacct
//
LOCUS       DAWEWP010000277          487 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWEWP010000277 DAWEWP010000000
VERSION     DAWEWP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.277"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..106)
                     /locus_tag="WGN53_002057"
     CDS             complement(<1..106)
                     /locus_tag="WGN53_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0140977.1"
                     /translation="MRKSRLSQYKQNKLIELFVTGVTARTAAELVGVNK"
     gene            173..355
                     /locus_tag="WGN53_002058"
                     /pseudo
     CDS             173..355
                     /locus_tag="WGN53_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          147 a          103 c           87 g          150 t
ORIGIN      
        1 ttttattaac gcctactaac tctgctgccg ttcttgcagt tacacctgtg acaaacagtt
       61 caatgagttt attttgttta tactggctta gacgactttt tctcataggg ataattctaa
      121 cttaatttga atttccctag ttatctagga cagcccctta aaaaatatcc ctttgaaaga
      181 ttttgatgaa gtttgtgaaa agtgggagaa atcctttaaa gaaaatggaa aagaaattga
      241 atataatcaa aaatattcaa gaatttatgg tgaagttcgt ggagctggct ttaatggcga
      301 tatcggagaa aagctacatc gttgtgccgt gatggaatgg gctgaaaatg agaccgagac
      361 ctttgcaaaa ttcctttccc tcccgacagc cgaaacccaa acacaggttt tcagctgttt
      421 tcagctgttt tcgccccaaa tacctcctaa ttttacccaa ataccccctt aatcctcccc
      481 ggacacc
//
LOCUS       DAWEWP010000278          477 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWEWP010000278 DAWEWP010000000
VERSION     DAWEWP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.278"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          132 a          110 c          102 g          133 t
ORIGIN      
        1 gcgggaatga cggcggagag ggtttgttgt ttcggataaa tccctgtagt ttttcgtttc
       61 tagattcccg cttttgcggg aatgacggcg gggggatttc tgtttttccg acaaattcct
      121 aaaacttaaa attccgttat tcccgcgaaa acagaaaacc aaaaatagaa acctaaaatc
      181 tcgtcattcc cgcaaaaaca gaaaaccaaa aaacagcaac ctaaaattcg tcattcccgc
      241 gcaggcggga atccagtccg ttcggtttcg cttgttttaa gtttcgggca acttccactt
      301 cgtcattccc gtgaaagtgg gaatccagtc cgttgagttt cagtcatttc cgataaatca
      361 ccgcaacgtt aaatttctag attcccgctt ttgcgggaat gacggcgggg gggatttctg
      421 tttttccgac aaattcctaa aacttaaaat tccgttattc ccgcgagaac agaaaac
//
LOCUS       DAWEWP010000279          452 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWEWP010000279 DAWEWP010000000
VERSION     DAWEWP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.279"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1..452
                     /locus_tag="WGN53_002059"
                     /pseudo
     CDS             1..452
                     /locus_tag="WGN53_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          145 a          119 c          111 g           77 t
ORIGIN      
        1 ataccctgtc cgaattgctg gaactgatta actgccaact gaccgaaaaa ggcttaaaag
       61 tagagaaagc attgacgcca ccattattca gaccgctggc agcaaacagc gtcaggccat
      121 agaagttgac gaagaaggac aaatcagcgg ccaaaccaca ccgagtaaag acagcgatgc
      181 ccgttggata aagaaaaacg gcctctacaa actcggttac aaacaacata cccgtaccga
      241 tgtggaaggc tatatcgaga aactgcacat tacccccgcc aatgcccatg agtgcaaaca
      301 cctgccgcct ttgctggaag ggatagctga aggtacgacc gtctatgccg acaaaggcta
      361 tgacagtgcg gaaaaccggc aacatctgga agagcgtcag ttgttagacg gcattatgcg
      421 caaagcctgc cgcaaccgtc cgctgtcgga ag
//
LOCUS       DAWEWP010000280          450 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWEWP010000280 DAWEWP010000000
VERSION     DAWEWP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.280"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          134 a          111 c           92 g          113 t
ORIGIN      
        1 taaagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca
       61 ctatatccgt ttgcgcccgc caagccgtgt tgcacagatg cgacacggct tttttctttc
      121 tgttgtcaga atgtaaattt tcgtcaaaaa acatcaaaaa tcaccccccc ccgaatttgc
      181 gtaaaataaa gtgtaatccc tttgatcata agggaatgtt gacaattaga tcgattctcc
      241 taggctgact ttcgcaataa atggggttgt caataattgt gtatggtcgt ttgccgtccg
      301 gcggccgtca ttcccgcgaa cgcaggaatc cagcaacaca atgcagcagg actttatcgg
      361 aaaaaaccga aaccccaccg actgtcattc ccgcaaaagc tggaatccaa aaacgcaacg
      421 caacaggaat ttatcggaaa aaacagaacc
//
LOCUS       DAWEWP010000281          422 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.281, whole genome shotgun sequence.
ACCESSION   DAWEWP010000281 DAWEWP010000000
VERSION     DAWEWP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.281"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>422
                     /locus_tag="WGN53_002060"
     CDS             <1..>422
                     /locus_tag="WGN53_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140978.1"
                     /translation="TNDTYDHLAKNIYQNLLFQTRLQPYLNQISFKMENDTFTLDFSG
                     LVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKV
                     LGQETVALLAKTSGTQADDILQNVGFGHNKNVSLYGNDG"
BASE COUNT          138 a           76 c           91 g          117 t
ORIGIN      
        1 accaacgata catacgacca tctcgccaaa aacatctacc aaaacctgtt gttccaaacc
       61 cgtttgcagc catatttgaa tcaaatcagt ttcaaaatgg aaaatgatac gttcactttg
      121 gattttagtg gtcttgttca agcatttaac catgtcaaag aaactaatcc gcaaaaagct
      181 tttgtggatt tggccgagat gcttgcatat ggcgaacttc gttcttggta tgaaggccga
      241 agactaatgg ccgattatgt ggaggaggca aaaaaagcag gtaaatttga agattaccag
      301 aaagtgttgg gtcaggagac cgttgcatta ttagctaaaa catcgggtac gcaagcagat
      361 gatatcctgc aaaatgtagg ctttggtcat aataaaaatg tttctttata tggtaatgac
      421 gg
//
LOCUS       DAWEWP010000282          420 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.282, whole genome shotgun sequence.
ACCESSION   DAWEWP010000282 DAWEWP010000000
VERSION     DAWEWP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.282"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..353)
                     /locus_tag="WGN53_002061"
     CDS             complement(<1..353)
                     /locus_tag="WGN53_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140979.1"
                     /translation="MSANLVQPLNFSDGISLHDCYCTEIKVEGRNVCFDFEDGFVVPD
                     NNPNNQAGKHLKTDFSRVVLTHKNENGEDDYLVDIFEDIVFLGKRLFTVRKFLDFSEL
                     LEMVNTQGYFLEFLY"
     gene            352..>420
                     /locus_tag="WGN53_002062"
     CDS             352..>420
                     /locus_tag="WGN53_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0140980.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAP"
BASE COUNT          157 a           87 c           66 g          110 t
ORIGIN      
        1 aaatacagaa actcaagaaa ataaccttgt gtattaacca tctcaagcaa ttcagaaaaa
       61 tcaagaaatt ttctgaccgt aaacaaacgt ttccctaaaa aaacgatgtc ttcaaaaata
      121 tcgaccaaat aatcatcttc gccgttttca tttttatggg ttaaaacaac acgggaaaaa
      181 tctgttttca gatgcttgcc cgcttgattg ttcggattat tgtccggaac gacaaaaccg
      241 tcctcaaaat caaagcagac gttgcgtcct tctaccttta tctctgtgca ataacaatca
      301 tgcagagaaa taccatccga aaaatttagg ggctgtacta gattagcaga tatgttaccc
      361 tcgaaatatg aagataacgc actgcaaatt aaagaaaaaa gtacagaaag aactgctccg
//
LOCUS       DAWEWP010000283          413 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.283, whole genome shotgun sequence.
ACCESSION   DAWEWP010000283 DAWEWP010000000
VERSION     DAWEWP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.283"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          152 a          114 c           83 g           64 t
ORIGIN      
        1 attcccgcaa aagcgggaat ccaaaaacgc aacgcaacag gaatccatcg gaaaaaaccg
       61 aaaccccacc gaccgtcatt cccgcaaaag cgggaatcca gacccgtcgg cacggaaact
      121 taccggataa aacagtttcc ttagattcca cgtcctagat tcccgccttc gcgggaatga
      181 cgagatttta gattatggga atttatcagg aatgattgaa tccatagaaa aaccacagga
      241 atctatcaga aaaaacagaa accccccacc gcgtcattcc cgcgcaggcg ggaatccaaa
      301 aacgcaacgc aacaggaatt tatcggaaaa aacagaaacc tcaccgccgt cattcccgca
      361 aaagcgggaa tctagaaaca caacgcggca ggactttatc agaaaaaaca gaa
//
LOCUS       DAWEWP010000284          385 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.284, whole genome shotgun sequence.
ACCESSION   DAWEWP010000284 DAWEWP010000000
VERSION     DAWEWP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.284"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            100..306
                     /locus_tag="WGN53_002063"
     CDS             100..306
                     /locus_tag="WGN53_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140981.1"
                     /translation="MFDGAKVVRRVEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          123 a           64 c           89 g          109 t
ORIGIN      
        1 gttaacagat tatttttgca aaggtctcag attaggcggt gtgccgtaat cgtacgaatg
       61 ccgattcaac ctaagcagac atcagtattt aggaagtgga tgtttgatgg agcaaaggtt
      121 gtacgaaggg tggaaggcaa cctgtgggtg tttggtatgg tcgcgcttga aaaaacgtgt
      181 tttaagggac aaatgccgtc tgaaaatcgg tttcagacgg cattttctgt ttatttaaag
      241 caaacaggaa aaggcagcaa tattctgcag tcttcctatt cacacaagcg ttttatagtt
      301 aattaaaaac aaaatagtac aatactcaac tttgaaggtc taaccatggc atactctgcg
      361 gacttaagaa acaaagcttt aaact
//
LOCUS       DAWEWP010000285          377 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.285, whole genome shotgun sequence.
ACCESSION   DAWEWP010000285 DAWEWP010000000
VERSION     DAWEWP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.285"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>377
                     /locus_tag="WGN53_002064"
     rRNA            <1..>377
                     /locus_tag="WGN53_002064"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           93 a           89 c          113 g           82 t
ORIGIN      
        1 aattgaccgc ctggggagta cggtcgcaag attaaaactc aaaggaattg acggggaccc
       61 gcacaagcgg tggatgatgt ggattaattc gatgcaacgc gaagaacctt acctggtctt
      121 gacatgtacg gaatcctcca gagacggagg agtgccttcg ggagccgtaa cacaggtgct
      181 gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac
      241 ccttgtcatt agttgccatc attcagttgg gcactctaat gagactgccg gtgacaagcc
      301 ggaggaaggt ggggatgacg tcaagtcctc atggccctta tgaccagggc ttcacacgtc
      361 atacaatggt cggtaca
//
LOCUS       DAWEWP010000286          373 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.286, whole genome shotgun sequence.
ACCESSION   DAWEWP010000286 DAWEWP010000000
VERSION     DAWEWP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.286"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1..373
                     /locus_tag="WGN53_002065"
                     /pseudo
     CDS             1..373
                     /locus_tag="WGN53_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          117 a          111 c           76 g           69 t
ORIGIN      
        1 aactcgaaca cagcctcatt acccgcatcg atttcaacct gttttgccgt tttgacgaac
       61 tgagcatccc tgattacagc accttatgcc gctaccgcaa ttggctggcg caagacgaca
      121 ccctgtccga attgctcaaa ctgatcaacc gccaactgac cgaaaaaggt ttaaaaatag
      181 agaaagcatc cgctgccgtc gttgacgcca ccattattca gaccgccggc agcaaacagc
      241 gtcaggccat agaagttgac gaataaggac aaatcaacgg ccaaaccaca ccgagtaagg
      301 acagcgatgc ccgttggatc aagaaaaacg gcctctacaa actcggttac aaacaacata
      361 cccgtaccga tgc
//
LOCUS       DAWEWP010000287          366 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.287, whole genome shotgun sequence.
ACCESSION   DAWEWP010000287 DAWEWP010000000
VERSION     DAWEWP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.287"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          123 a           87 c           59 g           97 t
ORIGIN      
        1 gtcttttcta aaatatgcag atattcgaat atcgaatttg ccttatgatt acggttttct
       61 gtcttatctg ccttaaaacg ccggtattcc gtttgaacca aattatattt gccgaaacac
      121 tcaaaaatct cccttacctg cccgacagac atcaagcctt cgttgttgta gctgagaaaa
      181 atatattgaa agtccgcatc cctaatcaaa gcctcaaata ccggaaccac atccgatttt
      241 gagcaagtcc gcgctaaaac acatcgtaca gcgcaaaacc ggatgggaat ggcaaaatcc
      301 ggaaacaaac atttttcaaa gcatacaaac aaaaaaatac caaccggagg ttggtattcc
      361 ttaact
//
LOCUS       DAWEWP010000288          364 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.288, whole genome shotgun sequence.
ACCESSION   DAWEWP010000288 DAWEWP010000000
VERSION     DAWEWP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.288"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..283)
                     /locus_tag="WGN53_002066"
     CDS             complement(<1..283)
                     /locus_tag="WGN53_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0140982.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPS"
BASE COUNT           97 a           91 c          108 g           68 t
ORIGIN      
        1 aagaaggaac gtatgtaccc gaaacggcag gtgcagacgg cggaacataa gcaggaggcg
       61 tataaaccgg cgcgctttgc accggcggca cataaggcgc atcgccggca ggagccgggc
      121 tgtacggcgt tgctccatag gggttgttgt aaactgccga agacggcgcg tcctgcatac
      181 ctgaattgcc tgcaatgaca ggagcaggct gttgggtggc gcaaccgccc aacagtgcgg
      241 caaaggaggc gcaagccgcc aaaagtgtcg tttgtttcaa cataagataa ccttcatgtt
      301 ccgatatata gcctgaatgc ggtatatcat aataaaaatg cgcgttcttc tcaagcgcaa
      361 agcc
//
LOCUS       DAWEWP010000289          361 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.289, whole genome shotgun sequence.
ACCESSION   DAWEWP010000289 DAWEWP010000000
VERSION     DAWEWP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.289"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           90 a           66 c           79 g          126 t
ORIGIN      
        1 ctctatcaaa ggggcgcatt acttttctta acattcccct ttgacagcca agtgaaaggg
       61 gcttttttat gtcagtagca aatgtaatat tttcttgttc ctattggaga atatttaaaa
      121 aatcagatta ttgcgtttta tgtttctatc agttcaggca tggtgaaccg cataaactcg
      181 ctgaacaaga aaatttttca aagctttatc aggcgttcga ttatatagat tcggttggct
      241 cgaattttcc ggtaattatc acaacagacg gttgtggtct ttcttcttga tctttaacag
      301 tttgtcagga ttgggctttc ggtcgttgac cgttggacgc gctttagcgc ggcagacggg
      361 a
//
LOCUS       DAWEWP010000290          360 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.290, whole genome shotgun sequence.
ACCESSION   DAWEWP010000290 DAWEWP010000000
VERSION     DAWEWP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.290"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            32..>360
                     /locus_tag="WGN53_002067"
     CDS             32..>360
                     /locus_tag="WGN53_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0140983.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSD"
BASE COUNT          112 a           97 c           86 g           65 t
ORIGIN      
        1 cgcacaaatt tagtcaatat caagaccaat tatgaaccaa ctcgaccaac ttggcacccg
       61 tatcaacctg atttgcaatg tcttcgacaa atggatcggg cagcaggatc tgaattacaa
      121 cctctttgcc gtactttata ccctggcaac cgaaggcagc cgcacgcaaa agcatatcgg
      181 cgaaaagtgg agcctgccca aacagaccgt ttcaggcgta tgcaaaaccc ttgccggaca
      241 agggttgatt gaatggcagg aaagcgaaca ggaccggcgc gaacggttgc tgtcgctgac
      301 cgaaaaaggc aaagcccatg ccgcaccttt aacagaaagc gcgcaggaat tcagcgacaa
//
LOCUS       DAWEWP010000291          348 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.291, whole genome shotgun sequence.
ACCESSION   DAWEWP010000291 DAWEWP010000000
VERSION     DAWEWP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.291"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           82 a           81 c          107 g           78 t
ORIGIN      
        1 gcaaagccat ctgaaggcga tgtgtttgaa cctgttgaaa gccgccaaca ggctaagtgc
       61 gcctgttgcc gcctaaaagg cagcacggat gcctgattat cgggtatccg gggaggatta
      121 agggggtatt ggggtaaaat taggaggtat ttggggcgga aatagctgaa aacctgtgtt
      181 tgggtttcgg ctgtcgggaa ggaaaggaat tttgcaaagg tctcgggcta tgccggctgc
      241 tgccgcccat tgcaccttcg ccgaatcctg ccgcccaccg ccgaagccct catgcgttcg
      301 cggtacagcg cgtatgtgct actatagtgg attaacaaaa accagtac
//
LOCUS       DAWEWP010000292          347 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.292, whole genome shotgun sequence.
ACCESSION   DAWEWP010000292 DAWEWP010000000
VERSION     DAWEWP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.292"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          122 a           85 c           62 g           78 t
ORIGIN      
        1 actcattagt tacctaaaac ttaaaaaaca gaaaccttta cgccgtcatt cccacgaaag
       61 tgggaatcta gaacccaaat gctaaggcga tttatcggaa acggctgaaa ccgaacgaat
      121 tggattcccg ccttcgcagg aatgacaact cattagttac ctaaaactta aaaaacagaa
      181 acctttacac cgtcattccc acgaaagtgg gaatctagaa cccaaatgct aaggcgattt
      241 atcggaaacg gctgaaaccg aatgaattgg attcccgcct gtgcgggaat gacgacttat
      301 tagttaccta acacttaaaa aacagaaacc tttccgcgtc attccca
//
LOCUS       DAWEWP010000293          332 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.293, whole genome shotgun sequence.
ACCESSION   DAWEWP010000293 DAWEWP010000000
VERSION     DAWEWP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.293"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           96 a           73 c           71 g           92 t
ORIGIN      
        1 tttttgaatt tcgggcaacg cttgaatctt cattccgcgc aggcggaaat tatcggtgcg
       61 gtacggcaac ttttttcgat atgaaaagac cgtcattcct gtaaaaacaa aaaatcaaaa
      121 acagaaaatt gaaattcgtc attcccgcgc aggcgggaat ctaggacgta aaatctatag
      181 tggattaaca aaaaccagta cagcgttgcc ttgacttagc tcgaagagaa cgattctcta
      241 aggtgctgaa gcaccgagtg agtcggttcc gcactatccg tactgtctgc ggcttcgtcg
      301 ctttctcctg atttttgtta atccactata aa
//
LOCUS       DAWEWP010000294          326 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.294, whole genome shotgun sequence.
ACCESSION   DAWEWP010000294 DAWEWP010000000
VERSION     DAWEWP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.294"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           90 a           55 c           73 g          108 t
ORIGIN      
        1 tttctgtttt tgattttctg ttttcgtagg aatgacggtt taaaagttac ccgaaacctc
       61 aaaaaaaagg ctgtgtttta atatagtgtt gatagacgta cttggcttcc atgtattcaa
      121 tcgtggaaat ctatatcttc gtcctcgccg aaatagtcta tgcccgatat acaattttga
      181 tacacaaact tggaaatatg ggtatcgtcg ccggagcgat agaatgcgga cagttatata
      241 tatacggttt ttttagggaa gcgggcagat gaagggcgga ttttgaatta accccatgca
      301 aattgacttt tgcaggcggt tttacc
//
LOCUS       DAWEWP010000295          322 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.295, whole genome shotgun sequence.
ACCESSION   DAWEWP010000295 DAWEWP010000000
VERSION     DAWEWP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.295"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           82 a           81 c           70 g           89 t
ORIGIN      
        1 ttggattccc gcttgcgcgg gaatgacggt agagcggttt cagttgctcc cgataaatgc
       61 cgcaatctca aatcccgtca ttccctcaaa aacagaaaaa caaaatcaga aacctaaaat
      121 cccgtcattc ccgcagctct tcgtcattcc cgtgaaagcg ggaatctaga ccttttaaat
      181 tttcacacgg cttttgaata ttgccgttac ccgaaggtct ggattcccgc ctgcacggga
      241 atgacggcag agcggtttca gttgtttcta aataaatttt tgcagcgttg gatttttaga
      301 ttcccgcttt cgcgggaatg ac
//
LOCUS       DAWEWP010000296          316 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.296, whole genome shotgun sequence.
ACCESSION   DAWEWP010000296 DAWEWP010000000
VERSION     DAWEWP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.296"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           87 a           85 c           64 g           80 t
ORIGIN      
        1 tcagatttcc gtgtttcgcg tttccggcaa ttcaagattg gcaaaacacg tcagcctgct
       61 tcgcaaggca ggtttttaaa actttatcag gcgttcgggc atatcggttc gaccggtccg
      121 catttccggc aatcaacact gccgacggtt gccgcatttc tttttgatat acaatcacac
      181 tttcaaaaaa catacgcagg caacaaaatc atcctccccc gattggcaaa cattccggca
      241 aattaaaacc ccggcggatt cgcatttgaa gtgcaacttt ccctaacaga aaaaggccag
      301 tatgcggtag catacg
//
LOCUS       DAWEWP010000297          314 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.297, whole genome shotgun sequence.
ACCESSION   DAWEWP010000297 DAWEWP010000000
VERSION     DAWEWP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.297"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            28..>314
                     /locus_tag="WGN53_002068"
     CDS             28..>314
                     /locus_tag="WGN53_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ0140984.1"
                     /translation="MQADLAYAAERITHDYPKATGANNTSTVSDYFRNIRAHSIHPRV
                     SVGYDFGDWRIAADYASYRKWNNNKYSVNTKELENKLNNKKDLKTENQENGT"
BASE COUNT          109 a           74 c           74 g           57 t
ORIGIN      
        1 gaagacagcg ggcacggccc gtattatgtg caggcggatt tagcttatgc cgccgaacgt
       61 attacccacg attatccgaa agcaaccggt gcaaacaaca caagcacagt aagcgattat
      121 ttcagaaaca tccgtgcgca ttccatccac ccccgggtgt cggtcggcta cgatttcggc
      181 gactggagaa tagcggcaga ttatgccagt tacagaaaat ggaacaacaa taaatattcc
      241 gtcaacacaa aagagttgga aaacaagctt aacaataaga aagacctgaa gacggaaaat
      301 caggaaaacg gtac
//
LOCUS       DAWEWP010000298          306 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.298, whole genome shotgun sequence.
ACCESSION   DAWEWP010000298 DAWEWP010000000
VERSION     DAWEWP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.298"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1..86)
                     /locus_tag="WGN53_002069"
                     /pseudo
     CDS             complement(1..86)
                     /locus_tag="WGN53_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT           79 a           51 c          106 g           70 t
ORIGIN      
        1 gctaaaaaat aggggaacct gtaaaggaag gggcatcggc cgccgtcggt gcttttttgt
       61 ttggaaggga aatggctgga aaaagacctg cgctatatcg gggaatgaat cgacgcaaag
      121 aaaatgccgt ctgaacatgc gttcgggcgg cgttttgttg gggggtatcg gagcggaacg
      181 tctgaaaaag ggtttcaggc ggtctttggg cgtgtggtga cagtcgaaaa cgtgataagg
      241 ctacctgaaa agtttgggag attttcaggt agcctttggt attgggcgca acagacgcag
      301 gtacag
//
LOCUS       DAWEWP010000299          298 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.299, whole genome shotgun sequence.
ACCESSION   DAWEWP010000299 DAWEWP010000000
VERSION     DAWEWP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.299"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>298
                     /locus_tag="WGN53_002070"
     CDS             <1..>298
                     /locus_tag="WGN53_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0140985.1"
                     /translation="AAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETDSNWGK
                     KSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSDKAKAAIDAARDRIAVLD"
BASE COUNT           88 a           73 c           68 g           69 t
ORIGIN      
        1 gctgccgcat tgtccggcga tttggccaat atgctgaaag cttattctgc cgccgaaact
       61 aaagaagcac agttggcatt gttagataat ttgattcaca aatgggcgga aaccgattcg
      121 aactggggca aaaaatcgcc aatgcgactt tcaaccgatt ggacgcaaac ggctaatgaa
      181 ggtattgcac tgacaccatc ccaagtagca caactaaaaa agaacgcttt agtttccctt
      241 tctgataaag ctaaagcagc tattgacgcc gcccgcgacc gcattgccgt gcttgatg
//
LOCUS       DAWEWP010000300          289 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.300, whole genome shotgun sequence.
ACCESSION   DAWEWP010000300 DAWEWP010000000
VERSION     DAWEWP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.300"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           67 a           67 c           76 g           79 t
ORIGIN      
        1 gggattttag cttgtgggta tttaccggaa aaaacagaaa ccgttccgcc gtcattcccg
       61 cgcaggcggg aatctaggtc tgtcggcacg gaaacttatc gggtaaaacg gtttcttgag
      121 attttacgtt ctagattccc actttcgtgg gaatgatggc agagcggatt ctgttgctcc
      181 cgataaatgc cgcaacctca aatcccgtca ttcccgcgca ggcgggaatc taggtctgtc
      241 ggcacagaaa cttatcgggt aaaacggttt cttgagattt tacgttctg
//
LOCUS       DAWEWP010000301          279 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.301, whole genome shotgun sequence.
ACCESSION   DAWEWP010000301 DAWEWP010000000
VERSION     DAWEWP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.301"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           93 a           65 c           67 g           54 t
ORIGIN      
        1 tggatggatt cgtcgttccc gcgaaagcgg gaatccggaa ccagaaacca caaaaattta
       61 tcgggaaaac cgaaactcaa aaacaacgga aaccgaacgg acaggattcc cgcctgcgcg
      121 ggaatgacgg ctcatacatc acccaaaaaa ttgaaaccaa acagatggga ttccctcttg
      181 cgtgaggctg acagatgccg tctgaaagac tttcagacgg catagctttt tctctttgaa
      241 tttatagtgg attaacaaaa atcaggacaa ggcggcgag
//
LOCUS       DAWEWP010000302          275 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.302, whole genome shotgun sequence.
ACCESSION   DAWEWP010000302 DAWEWP010000000
VERSION     DAWEWP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.302"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           63 a           62 c           56 g           94 t
ORIGIN      
        1 tctaaaaaat ttatgctatt aaatcagtaa tttctgatga attttgaaaa cttaatcccg
       61 tcattcccgc gcaagcggga atccggctcg ttcggtttcg cttgttttaa gtttcgggta
      121 acttccactt cgtcattccc gcacaggcgg gaatctggtt cgttcggtct cgcttgtttt
      181 aagtttcggg taacttccac ttcgtcattc ccgcgcaggc gggaatccaa tgcgttgagt
      241 ttcagctatt tagaataaat tttgaaactc taatc
//
LOCUS       DAWEWP010000303          265 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.303, whole genome shotgun sequence.
ACCESSION   DAWEWP010000303 DAWEWP010000000
VERSION     DAWEWP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.303"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..158
                     /locus_tag="WGN53_002071"
     CDS             <1..158
                     /locus_tag="WGN53_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0140986.1"
                     /translation="VEQSFGTLHRKFRYARVAYFGLIKVSAQSHLKAMCLNLLKAANR
                     LSAPAAA"
BASE COUNT           61 a           58 c           87 g           59 t
ORIGIN      
        1 tggtcgaaca gagcttcggt acgctgcacc gtaaattccg ctatgcccgg gtagcctatt
       61 tcgggctgat taaagtgagt gcgcaaagcc acctgaaggc gatgtgtttg aacctgttga
      121 aagcggccaa caggctaagt gcgcccgctg ccgcctaaaa agcggcccgg atgcctgatt
      181 atcgggtgtc cgggcaggat taagggggtg tttgggtaaa attaggcggt atttggggcg
      241 aaaacagctg aaaacctgtg ttggg
//
LOCUS       DAWEWP010000304          263 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.304, whole genome shotgun sequence.
ACCESSION   DAWEWP010000304 DAWEWP010000000
VERSION     DAWEWP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.304"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           79 a           61 c           49 g           74 t
ORIGIN      
        1 acggcggggg gatttctgtt tttccgataa attcctaaaa cttaaaattc cgttatttcc
       61 gcgagaacag aaaaccaaaa acagaaacct aaaatctcgt cattcccacg caggcgggaa
      121 tccagtctgt tgagtttcag tcatttccga taaatcaccg caacattaaa tttccagatt
      181 cccgcctgcg cgggaatgac ggtgggggga tttctgtttt tccgacaaat tcctaaaact
      241 taaaatttca tcatttctac gaa
//
LOCUS       DAWEWP010000305          263 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.305, whole genome shotgun sequence.
ACCESSION   DAWEWP010000305 DAWEWP010000000
VERSION     DAWEWP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.305"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            31..201
                     /locus_tag="WGN53_002072"
     CDS             31..201
                     /locus_tag="WGN53_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ0140987.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT           72 a           76 c           71 g           44 t
ORIGIN      
        1 atccgctata caaaaaacaa ggaaacacaa atggcgcaat atatgtgcgg cccctgcggc
       61 tggatttacg atgaagaaca cggcgacccc gaacacggca tcccccctgg gacaaagttt
      121 gaagacatcc ccgacgactg gaaatgcccc gaatgcggcg tgggcaaaga agatttctac
      181 ctgttggatt tcgtgatata gcggcagccg cacccttagg gaaaaccccg tcatccggcg
      241 ttccgcctgc ggcggcaggg aac
//
LOCUS       DAWEWP010000306          255 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2161204-rid20301733.denovo.306, whole genome shotgun sequence.
ACCESSION   DAWEWP010000306 DAWEWP010000000
VERSION     DAWEWP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2161204
            Sequence Read Archive: ERR369537
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995280.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995280.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 18:00:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,072
            CDSs (total)                      :: 2,013
            Genes (coding)                    :: 1,934
            CDSs (with protein)               :: 1,934
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 31 of 79
            Pseudo Genes (incomplete)         :: 36 of 79
            Pseudo Genes (internal stop)      :: 18 of 79
            Pseudo Genes (multiple problems)  :: 5 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2161204-rid20301733.denovo.306"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           64 a           65 c           62 g           64 t
ORIGIN      
        1 tgtcggtgcg gaaacttatc ggataacacg gtttccccaa ccctgcatcc tggattccca
       61 ctttcgtggg aatgacggcg gagcggtttc tgctttttcc aataaatgcc ccccaaacta
      121 aaatccgtca ttcccgcgca ggcgggaatc tagacattca atgctaaggc actttatcgg
      181 gaatgactga aactcaaaaa actggattcc cactgtcgtg ggaatgacgt ggtgcaggtt
      241 tccgtacgaa tggat
//
